1.ApplicationNumber: US-64518703-A
1.PublishNumber: US-2004191222-A1
2.Date Publish: 20040930
3.Inventor: EMINI EMILIO A.
SHIVER JOHN W.
BETT ANDREW J.
CASIMIRO DANILO R.
KASLOW DAVID C.
CHASTAIN MICHAEL

4.Inventor Harmonized: EMINI EMILIO A(US)
SHIVER JOHN W(US)
BETT ANDREW J(US)
CASIMIRO DANILO R(US)
KASLOW DAVID C(US)
CHASTAIN MICHAEL(US)

5.Country: US
6.Claims:
(en)Adenoviral serotypes differ in their natural tropism. The various serotypes of adenovirus have been found to differ in at least their capsid proteins (e.g., penton-base and hexon proteins), proteins responsible for cell binding (e.g, fiber proteins), and proteins involved in adenovirus replication. This difference in tropism and capsid proteins among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins. The present invention bypasses such requirement for capsid protein modification as it presents a recombinant, replication-defective adenovirus of serotype 34, a rare adenoviral serotype, and methods for generating the alternative, recombinant adenovirus. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided.

7.Description:
(en)CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. provisional application serial No. 60/458,825, filed on Mar. 28, 2003.



BACKGROUND OF THE INVENTION
[0002] Adenoviruses are nonenveloped, icosahedral viruses that have been identified in several avian and mammalian hosts; Home et al., 1959 J. Mol. Biol . 1:84-86; Horwitz, 1990 In Virology , eds. B. N. Fields and D. M. Knipe, pps. 1679-1721. The first human adenoviruses (Ads) were isolated over four decades ago. Since then, over 100 distinct adenoviral serotypes have been isolated which infect various mammalian species, 51 of which are of human origin; Straus, 1984, In The Adenoviruses , ed. H. Ginsberg, pps. 451-498, New York:Plenus Press; Hierholzer et al., 1988 J. Infect. Dis . 158:804-813; Schnurr and Dondero, 1993, Intervirology; 36:79-83; Jong et al., 1999 J Clin Microbiol ., 37:3940-5. The human serotypes have been categorized into six subgenera (A-F) based on a number of biological, chemical, immunological and structural criteria which include hemagglutination properties of rat and rhesus monkey erythrocytes, DNA homology, restriction enzyme cleavage patterns, percentage G+C content and oncogenicity; Straus, supra; Horwitz, supra.
[0003] The adenovirus genome is very well characterized. It consists of a linear double-stranded DNA molecule of approximately 36,000 base pairs, and despite the existence of several distinct serotypes, there is some general conservation in the overall organization of the adenoviral genome with specific functions being similarly positioned.
[0004] Adenovirus has been a very attractive target for delivery of exogenous genes. The biology of adenoviruses is very well understood. Adenovirus has not been found to be associated with severe human pathology in immuno-competent individuals. The virus is extremely efficient in introducing its DNA into the host cell and is able to infect a wide variety of cells. Furthermore, the virus can be produced at high virus titers in large quantities. In addition, the virus can be rendered replication defective by deletion of the essential early-region 1 (E1) of the viral genome; Brody et al, 1994 Ann N Y Acad Sci., 716:90-101.
[0005] Replication-defective adenovirus vectors have been used extensively as gene transfer vectors for vaccine and gene therapy purposes. These vectors are propagated in cell lines that provide E1 gene products in trans. Supplementation of the essential E1 gene products in trans is very effective when the vectors are from the same or a very similar serotype. E1-deleted group C serotypes (Ad1, Ad2, Ad5 and Ad6), for instance, grow well in 293 or PER.C6 cells which contain and express the Ad5 E1 region. However, the Ad5 E1 sequences in 293 or PER.C6 cells do not fully complement the replication of all serotypes other than group C. This is perhaps due to the inability of the Ad5 (group C) E1B 55K gene product to functionally interact with the E4 gene product(s) of the non-group C serotypes. Although the interaction is conserved within members of the same subgroup, it has not been found to be well conserved between subgroups. In order to successfully and efficiently rescue recombinant adenovirus of alternative, non-group C serotypes, a cell line expressing the E1 region of the serotype of interest would have to be generated. Alternatively, available Ad5E1-expressing cell lines could be modified to express Ad5E4 (or Orf6) in addition to Ad5E1. These additional, sometimes tedious and daunting tasks, impeded the production of recombinant, non-group C adenoviral vectors.
[0006] An efficient means for the propagation and rescue of alternative serotypes in an Ad5 E1-expressing cell line (such as PER.C6 or 293) was disclosed in pending U.S. provisional application (Serial No. 60/405,182, filed Aug. 22, 2002). This method involves the incorporation of a critical E4 region into the adenovirus to be propagated. The critical E4 region is native to a virus of the same or highly similar serotype as that of the E1 gene product(s), particularly the E1B 55K region, of the complementing cell line, and comprises, in the least, nucleic acid encoding E4 Orf6.
[0007] Presently, two well-characterized adenovirus serotypes from subgroup C, Ad5 and Ad2, are the most widely used gene delivery vectors. There is a need to develop alternate Ad serotypes as gene transfer vectors since neutralizing antibodies in the general population may limit primary dosing or redosing with the same serotype. The prevalence of neutralizing antibody can vary from serotype to serotype. Neutralizing antibodies to some serotypes such as Ad5 are common, while antibodies to others are relatively rare. Alternate serotypes, furthermore, possess alternate tropisms which may lead to the elicitation of superior immune responses when used for vaccine or gene therapy purposes.
[0008] Adenovirus serotype 34, a subgroup B adenovirus, was originally isolated in 1972 and established as a recognized reference strain in 1975 (J. C. Hierholzer et al., 1975 J. Clin. Microbiol. 1:366-376). Its antigenic relationship to 46 other human adenoviruses determined in reference horse antisera has been discussed; J. C. Hierholzer et al., 1991 Arch. Virol. 121:179-197. Partial sequence information is available for Ad34. There have been several disclosures relating to Ad34 hexon sequences. The complete sequence of Ad34 hexon with some 5′ and 3′ flanking sequence (3358 bp) was deposited in GenBank (Accession No. AB052911) by Mukouyama. A partial sequence of Ad34 hexon (1449 bp) was disclosed in Takeuchi et al., 1999 J. Clin. Microbiol. 37:3392-3394, and GenBank (Accession No. AB018426). A partial sequence of Ad34 hexon (253 bp) was disclosed in Allard et al, 2001 J. Clin. Microbiol. 39: 498-505 , and deposited in GenBank (Accession No. AF161573). Perera and Cardosa deposited two partial sequences of Ad34 hexon (571bp and 301 bp) with GenBank (Accession Nos. AJ272610 and AJ250786). Sequence for the Ad34 fiber gene was deposited by Arun, Mukouyama and Inada with GenBank (Accession No. AB073168). The sequence of the virus associated RNA region (VA RNA1 & 2) for Ad34 (162 bp) was disclosed by Kidd et al., 1995 Virology 207:32-45, and GenBank (Accession No. U10677). Moreover, the sequence of the virus associated RNA region for Ad34 and partial sequence for the pre-terminal protein and 52/55K proteins (354 bp) was disclosed in Ma & Matthews, 1996 J. Virol. 70: 5083-99, and GenBank (Accession No. U52571). Adhikary, Mukouyama and Inada disclosed the sequence of the Ad34 genes for L4 100kDa, L4pVIII, E3 12.3kDa, E3 14.9kDa, E3 gp18.5kDa, E3 20.3kDa, E3 20.5kDa, E3 10.2kDa, E3 15.2kDal, E3 15.2kDa2, and partial fiber sequence (4828 bp) and deposited the sequence with GenBank (Accession No. AB079724). The sequence of the right end of the viral genome (1038 bp) was disclosed in Chen & Horwitz, 1990 Virology 179:567-75, and GenBank (Accession No. M62712).
[0009] The fields of vaccines and gene therapy would greatly benefit from additional knowledge concerning alternative adenoviral serotypes, particularly those serotypes such as Ad34 which are not well represented in the human population. Of particular interest are recombinant adenoviral vectors based on alternative adenoviral serotypes, and means of obtaining such recombinant adenoviral vectors. This need in the art is met with the disclosure of the present application related to recombinant adenoviral vectors based on adenoviral serotype 34.
SUMMARY OF THE INVENTION
[0010] The present invention relates to recombinant, replication-deficient adenovirus vectors of serotype 34, a rare adenoviral serotype, and methods for generating the recombinant adenovirus based on the alternative serotype. Additionally, means of employing the recombinant adenovirus for the delivery and expression of exogenous genes are provided. The invention, thus, encompasses recombinant, replication-defective adenoviral vectors of serotype 34 which comprise one or more transgenes operatively linked to regulatory sequences which promote effective expression of the respective transgene(s). Host administration of such recombinant adenovirus serotype 34 vectors, whether administered alone or in a combined modality and/or prime boost regimen, results in the efficient expression of the incorporated transgene and effectively induces an immune response capable of specifically recognizing the particular antigen administered (e.g., HIV). Furthermore, the recombinant virus should evade pre-existing immunity to adenovirus serotypes which are more commonly encountered in the human population (e.g., Ad5 and Ad2). The disclosed methods, thus, present an enhanced means for inducing an immune response against a particular antigen of interest (e.g., HIV). Accordingly, the resultant immune response should offer a prophylactic advantage to previously uninfected individuals and/or provide a therapeutic effect by reducing viral load levels within an infected individual, thus prolonging the asymptomatic phase of infection.



BRIEF DESCRIPTION OF THE DRAWINGS
[0011]FIG. 1 illustrates the homologous recombination scheme utilized to recover pAd34ΔE1ΔE4Ad5Orf6.
[0012]FIG. 2 illustrates the homologous recombination scheme utilized to recover pMRKAd34ΔE1ΔE4Ad5Orf6.
[0013]FIGS. 3A-1 to 3 A- 9 illustrate a nucleic acid sequence for wild-type adenovirus serotype 34 (SEQ ID NO: 1). The ATCC product number for Ad34 is VR-716.
[0014]FIG. 4 illustrates the time course of SEAP expression using MRKAd5 and Ad34 vectors in rhesus macaques. Data represent cohort geometric means.
[0015]FIG. 5 illustrates, in tabular format, T cell responses induced using MRKAd5 and Ad34 vectors expressing HIV-1 gag. Data are expressed in numbers of spot-forming cells per million PBMC (SFC/10{circumflex over ( )}6 PBMC). “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.
[0016]FIG. 6 illustrates, in tabular format, the levels of CD4+ and CD8+ Gag-specific T cells in Ad34-immunized macaques at week 12. “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.
[0017]FIG. 7 illustrates the nucleic acid sequence (SEQ ID NO: 3) of the optimized human HIV-1 gag open reading frame.
[0018]FIG. 8 illustrates the nucleic acid sequence encoding the gag expression cassette (SEQ ID NO: 4). The various regions of the figure are as follows: (1) a first underlined segment of nucleic acid sequence encoding the immediate early gene promoter region from human cytomegalovirus; (2) a first segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; (3) a region in caps which contains the coding sequence of HIV-1 gag; (4) a second segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; and (5) a second underlined segment, this segment containing nucleic acid sequence encoding a bovine growth hormone polyadenylation signal sequence.
[0019]FIG. 9 illustrates the nucleic acid sequence encoding the SEAP expression cassette (SEQ ID NO: 5). The various regions of the figure are as follows: (1) a first underlined segment of nucleic acid sequence encoding the immediate early gene promoter region from human cytomegalovirus; (2) a first segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; (3) a region in caps which contains the coding sequence of the human placental SEAP gene; (4) a second segment of lowercase letters which is not underlined, which segment of DNA contains a convenient restriction enzyme site; and (5) a second underlined segment, this segment containing nucleic acid sequence encoding a bovine growth hormone polyadenylation signal sequence.
[0020]FIGS. 10A-1 to 10 A- 47 illustrate the nucleotide sequence of the pMRKAd5HIV-1 gag vector (SEQ ID NO: 6 [coding] and SEQ ID NO: 7 [non-coding]).
[0021]FIGS. 11A-1 to 11 A- 10 illustrate a nucleic acid sequence for wild-type adenovirus serotype 35 (SEQ ID NO: 13). The ATCC product number for Ad35 is VR-718.
[0022]FIG. 12 illustrates, in tabular format, T cell responses induced using a heterologous Ad34 prime/Ad35 boost regimen in macaques. “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.
[0023]FIG. 13 illustrates, in tabular format, the levels of CD4+ and CD8+ Gag-specific T cells in Ad34 primed/Ad35 boosted macaques at week 28. “a” refers to a 20-mer peptide pool with 10-aa overlap and encompassing the entire HIV-1 CAM1 gag.


DETAILED DESCRIPTION OF THE INVENTION
[0024] Rare adenoviral serotypes possess an inherent advantage over the more commonly exploited adenoviral serotypes (for instance, adenoviral serotypes 2 and 5) since preexisting immunity is unlikely to limit their efficient delivery and expression of exogenous genes to their target site. Different adenoviral serotypes also exhibit distinct tropisms by reason of their varying capsid structure and, thus, present the potential for targeting different tissues and possibly leading to the elicitation of superior immune responses when used for vaccine or gene therapy purposes. These rare adenoviral serotypes when rendered replication-defective, however, can be difficult to propagate and rescue in currently available adenoviral propagation cell lines.
[0025] Applicants have recently managed to successfully rescue and propagate one such rare, replication-defective alternative serotype, adenovirus serotype 34, a subgroup B adenovirus, and herein demonstrate the effective functioning of the adenovirus in the delivery and expression of exogenous transgenes.
[0026] Accordingly, the present invention relates to a recombinant adenoviral vector of serotype 34 suitable for use in gene therapy or vaccination protocols. The nucleic acid sequence for wild-type adenovirus serotype 34 (SEQ ID NO: 1) is illustrated in FIGS. 3A-1 to 3 A- 9 , although any functional homologue or different strain of adenovirus serotype 34 can be utilized in accordance with the methods of the present invention, as one of ordinary skill in the art will appreciate. Ad34 sequence has been noted to differ in a few regions. The following sites are just a sampling of sequence variation that can be found in Ad34: (1) around base pair 10640 of SEQ ID NO: 1, a series of thirteen (“13”) rather than twelve (“12”) “T”s follow the sequence gtgagtccta (SEQ ID NO: 8); (2) around base pair 15372 of SEQ ID NO: 1, a series of fifteen (“15”) or seventeen (“17”) rather than sixteen (“16”) “A”s follow the sequence ccgcactttct (SEQ ID NO: 9); (3) around base pair 17325 of SEQ ID NO: 1, a series of thirteen (“13”) rather than twelve (“12”) “A”s follow the sequence attgacattgg (SEQ ID NO: 10); and (4) around base pair 25717 of SEQ ID NO: 1, the sequence cagtctggagga (SEQ ID NO: 11) following the sequence ggagga (SEQ ID NO: 12) is deleted. Adenovirus serotypes have been distinguished through a number of art-appreciated biological, chemical, immunological and structural criteria which include hemagglutination properties of rat and rhesus monkey erythrocytes, DNA homology, restriction enzyme cleavage patterns, percentage G+C content and oncogenicity; Straus, supra; Horwitz, supra. A given serotype can be identified by a number of methods including restriction mapping of viral DNA; analyzing the mobility of viral DNA; analyzing the mobility of virion polypeptides on SDS-polyacrylamide gels following electrophoresis; comparison of sequence information to known sequence particularly from capsid genes (e.g., hexon) which contain sequences that define a serotype; and comparing a sequence with reference sera for a particular serotype available from the ATCC. Classification of adenovirus serotypes by SDS-PAGE has been discussed in Wadell et al., 1980 Ann. N. Y Acad. Sci. 354:16-42. Classification of adenovirus serotypes by restriction mapping has been discussed in Wadell et al., Current Topics in Microbiology and Immunology 110: 191-220. Adenovirus serotype 34, a subgroup B adenovirus, was originally isolated in 1972 and was established as a recognized reference strain in 1975 (J. C. Hierholzer et al., 1975 J. Clin. Microbiol. 1:366-376). Its antigenic relationship to 46 other human adenoviruses determined in reference horse antisera has been discussed in the art; J. C. Hierholzer et al., 1991 Arch. Virol. 121:179-197.
[0027] Adenovirus serotype 34 vectors in accordance with the present invention are at least partially deleted in E1 and devoid (or essentially devoid) of E1 activity, rendering the vector incapable of replication in the intended host. Preferably, the E1 region is completely deleted or inactivated. The adenoviruses may contain additional deletions in E3, and other early regions, albeit in situations where E2 and/or E4 is deleted, E2 and/or E4 complementing cell lines may be required to generate recombinant, replication-defective adenoviral vectors.
[0028] Adenoviral vectors of use in the methods of the present invention can be constructed using well known techniques, such as those reviewed in Hitt et al., 1997 “Human Adenovirus Vectors for Gene Transfer into Mammalian Cells” Advances in Pharmacology 40:137-206, which is hereby incorporated by reference. Often, a plasmid or shuttle vector containing the heterologous nucleic acid of interest is generated which comprises sequence homologous to the specific adenovirus of interest. The shuttle vector and viral DNA or second plasmid containing the cloned viral DNA are then co-transfected into a host cell where homologous recombination occurs and results in the incorporation of the heterologous nucleic acid into the viral nucleic acid. Preferred shuttle vectors and cloned viral genomes contain adenoviral and plasmid portions. For shuttle vectors used in the construction of replication-defective vectors, the adenoviral portion typically contains non-functional or deleted E1 and E3 regions and the gene expression cassette, flanked by convenient restriction sites. The plasmid portion of the shuttle vector typically contains an antibiotic resistance marker under the transcriptional control of a prokaryotic promoter. Ampicillin resistance genes, neomycin resistance genes and other pharmaceutically acceptable antibiotic resistance markers may be used. To aid in the high level production of the nucleic acid by fermentation in prokaryotic organisms, it is advantageous for the shuttle vector to contain a prokaryotic origin of replication and be of high copy number. A number of commercially available prokaryotic cloning vectors provide these benefits. Non-essential DNA sequences are, preferably removed. It is also preferable that the vectors not be able to replicate in eukaryotic cells. This minimizes the risk of integration of nucleic acid vaccine sequences into the recipients' genome. Tissue-specific promoters or enhancers may be used whenever it is desirable to limit expression of the nucleic acid to a particular tissue type.
[0029] Homologous recombination of the shuttle vector and wild-type adenovirus 34 viral DNA (Ad34 backbone vector) results in the generation of adenoviral pre-plasmids (see, for instance, pAd34ΔE1ΔE4Ad5Orf6, pMRKAd34ΔE1ΔE4Ad5Orf6, pAd34ΔE1gagΔE4Ad5Orf6, and pAd34ΔE1SEAPΔE4Ad5Orf6). Upon linearization, the pre-plasmids are capable of replication in PER.C6® cells or alternative E1-complementing cell lines. Infected cells and media can then be harvested once viral replication is complete.
[0030] A packaging cell will generally be needed in order to produce sufficient amount of adenovirus. The packaging cell should contain elements which are necessary for the production of the specific adenovirus of interest. It is preferable that the packaging cell and the vector not contain overlapping elements which could lead to replication competent virus by recombination. Specific examples of cells which are suitable for the propagation of recombinant Ad34 E1-deleted vectors express the early region 1 (E1) of adenovirus 34 or another group B serotype. Alternatively, propagation cell lines can be used which express adenoviral E1 and E4 regions (particularly, E4 open reading frame 6 (“ORF6”)) which are derived from the same serotype but different subgroup than Ad34 (e.g., Ad5 E1 and E4); see, e.g., Abrahamsen et al., 1997 J. Virol. 8946-8951, and U.S. Pat. No. 5,849,561. Additionally, a cell line could be used that expresses E1B from Ad34 in addition to (1) E1A or (2) E1A and E1B from a serotype of a different subgroup. In copending U.S. provisional application serial No. 60/405,182, filed Aug. 22, 2002, a strategy was disclosed for the efficient propagation and rescue of alternative adenoviral serotypes. The method is based on incorporating, into the genome of the adenovirus vector, an E4 region (or portion thereof including E4 ORF6) of the same or highly similar serotype as that of the E1 gene product(s), particularly E1B, being expressed by the complementing cell line. Examples 1-4 demonstrate the viability of such a method through the incorporation of an Ad5E4 region and its propagation in PER.C6 cells (which cells express Ad5E1). The wildtype adenovirus serotype 5 sequence is known and described in the art; see Chroboczek et al., 1992 J. Virol. 186:280, which is hereby incorporated by reference. Placement of the E4 region or ORF6-containing portion is not critical. The critical step is making sure that either a promoter is supplied or the gene is strategically placed so that it runs off a promoter native to the vector (e.g., such as the E4 promoter). The native E4 region of the vector can be replaced, deleted or left intact. This method is, thus, suitable for use in the propagation and rescue of the adenoviral vectors of the present invention.
[0031] Typically, propagation cells are human cells derived from the retina or kidney, although any cell line capable of expressing the appropriate E1 and/or E4 region(s) can be utilized in the present invention. Embryonal cells such as amniocytes have been shown to be particularly suited for the generation of E1 complementing cell lines. Several cell lines are available. These include but are not limited to the known cell lines PER.C6 (ECACC deposit number 96022940), 911, 293, and E1 A549.
[0032] The present invention encompasses methods for producing a recombinant, replication-defective adenovirus of serotype 34 in an adenoviral E1-complementing cell line, comprising transfecting a recombinant, replication-defective adenoviral vector of serotype 34 in an adenoviral E1-complementing cell and allowing for the production of viral particles. The viral particles so produced form another aspect of the present invention. Host cells comprising the recombinant, replication-defective adenoviral serotype 34 vectors of the present invention form yet another aspect of the present invention; host cells being defined as a population of cells not including a transgenic human being. Recombinant, replication-defective adenovirus harvested in accordance with the methods of the present invention are encompassed herein as well. This harvested material may be purified, formulated and stored prior to host administration.
[0033] Adenoviral vectors in accordance with the present invention are very well suited to effectuate expression of desired proteins, especially in situations where an individual's immune response effectively prevents administration or readministration via the more commonly employed adenoviral serotypes. Accordingly, specific embodiments of the present invention are recombinant, replication-defective adenoviral vectors of serotype 34 which comprise a heterologous nucleic acid of interest. The nucleic acid of interest can be a gene, or a functional part of a gene. The nucleic acid can be DNA and/or RNA, can be double or single stranded, and can exist in the form of an expression cassette. The nucleic acid can be inserted in an E1 parallel (transcribed 5′ to 3′) or anti-parallel (transcribed in a 3′ to 5′ direction relative to the vector backbone) orientation. The nucleic acid can be codon-optimized for expression in the desired host (e.g., a mammalian host). The heterologous nucleic acid can be in the form of an expression cassette. A gene expression cassette will typically contain (a) nucleic acid encoding a protein or antigen of interest; (b) a heterologous promoter operatively linked to the nucleic acid encoding the protein; and (c) a transcription termination signal.
[0034] In specific embodiments, the heterologous promoter is recognized by an eukaryotic RNA polymerase. One example of a promoter suitable for use in the present invention is the immediate early human cytomegalovirus promoter (Chapman et al., 1991 Nucl. Acids Res. 19:3979-3986). Further examples of promoters that can be used in the present invention are the strong immunoglobulin promoter, the EF1 alpha promoter, the murine CMV promoter, the Rous Sarcoma Virus promoter, the SV40 early/late promoters and the beta actin promoter, albeit those of skill in the art can appreciate that any promoter capable of effecting expression in the intended host can be used in accordance with the methods of the present invention. The promoter may comprise a regulatable sequence such as the Tet operator sequence. Sequences such as these that offer the potential for regulation of transcription and expression are useful in instances where repression of gene transcription is sought. The adenoviral gene expression cassette may comprise a transcription termination sequence; specific embodiments of which are the bovine growth hormone termination/polyadenylation signal (bGHpA) or the short synthetic polyA signal (SPA) of 50 nucleotides in length defined as follows: AATAAAAGATCTTTATTTTCATTAGATCTGTGTGTT-GGTTTTTTGTGTG (SEQ ID NO:2). A leader or signal peptide may also be incorporated into the transgene. In specific embodiments, the leader is derived from the tissue-specific plasminogen activator protein, tPA.
[0035] Heterologous nucleic acids of interest are genes (or their functional counterparts) which encode immunogenic and/or therapeutic proteins. Preferred therapeutic proteins are those which elicit some measurable therapeutic benefit in the individual host upon administration. Preferred immunogenic proteins are any proteins which are capable of eliciting an immune response in an individual. Applicants have exemplified the delivery of a representative immunogenic protein (HIV gag) in the present specification in non-human primates (rhesus macaques), albeit any gene encoding a therapeutic or immunogenic protein can be used in accordance with the methods disclosed herein. The adenovirus serotype 34 vectors were found to induce significant levels of gag-specific T cells; FIG. 5. Moreover, the results indicated that immunization with the disclosed vectors was able to elicit both HIV-specific CD4+ and CD8+ T cells; FIG. 6.
[0036] An aspect of the present invention, therefore, relates to adenovirus serotype 34-based vectors carrying an HIV transgene. In these embodiments, nucleic acid encoding any HIV antigen may be utilized (specific examples of which include gag, pol, nef, gp160, gp41, gp120, tat, and rev, including derivatives of the aforementioned genes). The embodiments exemplified herein employ nucleic acid encoding a codon-optimized p55 gag antigen; see FIG. 7 (SEQ ID NO: 3). Codon-optimized HIV-1 env genes are disclosed in PCT International Applications PCT/US97/02294 and PCT/US97/10517, published Aug. 28, 1997 (WO 97/31115) and Dec. 24, 1997, respectively. Codon-optimized HIV-1 pol genes are disclosed in U.S. application Ser. No. 09/745,221, filed Dec. 21, 2000 and PCT International Application PCT/US00/34724, also filed Dec. 21, 2000. Codon-optimized HIV-1 nef genes are disclosed in U.S. application Ser. No. 09/738,782, filed Dec. 15, 2000 and PCT International Application PCT/US00/34162, also filed Dec. 15, 2000.
[0037] In this specific embodiment of a recombinant, replication-defective Ad34 vector comprising an HIV-1 gene, the gene may be derived from HIV-1 strain CAM-1; Myers et al, eds. “Human Retroviruses and AIDS: 1995, IIA3-IIA19, which is hereby incorporated by reference. This gene closely resembles the consensus amino acid sequence for the lade B (North American/European) sequence. HIV gene sequence(s) may be based on various clades of HIV-1; specific examples of which are Clades B and C. Sequences for genes of many HIV strains are publicly available from GenBank and primary, field isolates of HIV are available from the National Institute of Allergy and Infectious Diseases (NIAID) which has contracted with Quality Biological (Gaithersburg, Md.) to make these strains available. Strains are also available from the World Health Organization (WHO), Geneva Switzerland. It is well within the purview of the skilled artisan to choose an appropriate nucleotide sequence which encodes a specific HIV antigen, or immunologically relevant portion or modification thereof. “Immunologically relevant” as defined herein means (1) with regard to a viral antigen, that the protein is capable, upon administration, of eliciting a measurable immune response within an individual sufficient to retard the propagation and/or spread of the virus and/or to reduce the viral load present within the individual; or (2) with regards to a nucleotide sequence, that the sequence is capable of encoding for a protein capable of the above.
[0038] The present invention encompasses methods for (1) effectuating a therapeutic response in an individual and (2) generating an immune response (including a cellular-mediated immune response) comprising administering to an individual a recombinant adenovirus serotype 34 vector in accordance with the present invention. One aspect of the present invention are methods for generating an enhanced immune response against one or more antigens (bacterial, viral (e.g., HIV), or other (e.g., cancer)) which comprise the administration of a recombinant adenovirus serotype 34 vehicle expressing the antigen of interest. Administration of recombinant Ad34 vectors in this manner provides for improved cellular-mediated immune responses, particularly where there is pre-existing immunity in a given host to the more well-represented adenovirus serotypes (e.g., Ad2 and Ad5). An effect of the improved vaccine administration methods should be a lower transmission rate to (or occurrence rate in) previously uninfected individuals (i.e., prophylactic applications) and/or a reduction in the levels of virus/bacteria/foreign agent within an infected individual (i.e., therapeutic applications). As relates to HIV indications, an effect of the improved vaccine administration methods should be a lower transmission rate to previously uninfected individuals (i.e., prophylactic applications) and/or a reduction in the levels of viral loads within an infected individual (i.e., therapeutic applications) so as to prolong the asymptomatic phase of HIV infection. Administration, intracellular delivery and expression of the recombinant Ad34 vectors elicits a host CTL and Th response.
[0039] Accordingly, the present invention relates to methodology regarding administration of the recombinant Ad34 viral vectors (or immunogenic compositions thereof, herein termed vaccines) to provide effective immunoprophylaxis, to prevent establishment of an infection following exposure to the viral (for instance, HIV), bacterial or other agent, or as a post-infection therapeutic vaccine to mitigate infection to result in the establishment of a lower virus/bacteria/other load with beneficial long term consequences.
[0040] The recombinant adenovirus serotype 34 vectors of the present invention may be administered alone, or as part of a prime/boost administration regimen. A priming dose(s) of at least one antigen (e.g., an HIV antigen) is first delivered with a recombinant adenoviral vector. This dose effectively primes the immune response so that, upon subsequent identification of the antigen(s) in the circulating immune system, the immune response is capable of immediately recognizing and responding to the antigen(s) within the host. The priming dose(s) is then followed with a boosting dose comprising a recombinant adenoviral vector containing at least one gene encoding the antigen. A mixed modality prime and boost inoculation scheme will result in an enhanced immune response, particularly where there is pre-existing anti-vector immunity. Prime-boost administrations typically involve priming the subject (by viral vector, plasmid, protein, etc.) at least one time, allowing a predetermined length of time to pass, and then boosting (by viral vector, plasmid, protein, etc.). Multiple primings, typically 1-4, are usually employed, although more may be used. The length of time between priming and boost may typically vary from about four months to a year, albeit other time frames may be used as one of ordinary skill in the art will appreciate.
[0041] In addition to a single protein or antigen of interest being delivered by the recombinant, replication-defective adenovirus serotype 34 vectors of the present invention, two or more proteins or antigens can be delivered either via separate vehicles or delivered via the same vehicle. Multiple genes/functional equivalents may be ligated into a proper shuttle plasmid for generation of a pre-adenoviral plasmid comprising multiple open reading frames. Open reading frames for the multiple genes/functional equivalents can be operatively linked to distinct promoters and transcription termination sequences. In other embodiments, the open reading frames may be operatively linked to a single promoter, with the open reading frames operatively linked by an internal ribosome entry sequence (IRES; as disclosed in WO 95/24485), or suitable alternative allowing for transcription of the multiple open reading frames to run off of a single promoter. In certain embodiments, the open reading frames may be fused together by stepwise PCR or suitable alternative methodology for fusing together two open reading frames. Due consideration must be given, however, to the effective packaging limitations of the viral vehicle. Adenovirus type 5, for instance, has been shown to exhibit an upper cloning capacity limit of approximately 105% of the wildtype Ad5 sequence.
[0042] Prime-boost regimens can employ different adenoviral serotypes. One example of such a protocol would be a priming dose(s) comprising a recombinant adenoviral vector of a first serotype followed by a boosting dose comprising a recombinant adenoviral vector of a second and different serotype; see, for instance, Example 6 and FIGS. 12 and 13. Therein, a cohort of monkeys was given two doses of an Ad34-based HIV gag vector at weeks 0 and 4, and boosted at week 24 with an Ad35-based HIV gag vector. Administration of the Ad35-based vector resulted in about a 3-fold enhancement in T cell responses when compared to the levels at the time of the booster. In an alternative embodiment, the priming dose can comprise a mixture of separate adenoviral vehicles each comprising a gene encoding for a different protein/antigen. In such a case, the boosting dose would also comprise a mixture of vectors each comprising a gene encoding for a separate protein/antigen, provided that the boosting dose(s) administers recombinant viral vectors comprising genetic material encoding for the same or similar set of antigens that were delivered in the priming dose(s). These multiple gene/vector administration modalities can further be combined. It is further within the scope of the present invention to embark on combined modality regimes which include multiple but distinct components from a specific antigen.
[0043] Compositions, including vaccine compositions, comprising the adenoviral vectors of the present invention are an important aspect of the present invention. These compositions can be administered to mammalian hosts, preferably human hosts, in either a prophylactic or therapeutic setting. Potential hosts/vaccinees include but are not limited to primates and especially humans and non-human primates, and include any non-human mammal of commercial or domestic veterinary importance. Compositions comprising recombinant adenoviral serotype 34 vectors may be administered alone or in combination with other viral- or non-viral-based DNA/protein vaccines. They also may be administered as part of a broader treatment regimen. The present invention encompasses those situations as well where the disclosed recombinant adenoviral serotype 34 vectors are administered in conjunction with other therapies; for example, HAART therapy (in the case of a recombinant HIV vector).
[0044] Compositions comprising the recombinant viral vectors may contain physiologically acceptable components, such as buffer, normal saline or phosphate buffered saline, sucrose, other salts and polysorbate. In certain embodiments, the formulation has: 2.5-10 mM TRIS buffer, preferably about 5 mM TRIS buffer; 25-100 mM NaCl, preferably about 75 mM NaCl; 2.5-10% sucrose, preferably about 5% sucrose; 0.01-2 mM MgCl 2 ; and 0.001%-0.01% polysorbate 80 (plant derived). The pH should range from about 7.0-9.0, preferably about 8.0. One skilled in the art will appreciate that other conventional vaccine excipients may also be used in the formulation. In specific embodiments, the formulation contains 5mM TRIS, 75 mM NaCl, 5% sucrose, 1 mM MgCl 2 , 0.005% polysorbate 80 at pH 8.0. This has a pH and divalent cation composition which is near the optimum for Ad5 and Ad6 stability and minimizes the potential for adsorption of virus to a glass surface. It does not cause tissue irritation upon intramuscular injection. It is preferably frozen until use.
[0045] The amount of viral particles in the vaccine composition to be introduced into a vaccine recipient will depend on the strength of the transcriptional and translational promoters used and on the immunogenicity of the expressed gene product. In general, an immunologically or prophylactically effective dose of 1×10 7 to 1×10 12 particles and preferably about 1×10 10 to 1×10 11 particles is administered directly into muscle tissue. Subcutaneous injection, intradermal introduction, impression through the skin, and other modes of administration such as intraperitoneal, intravenous, or inhalation delivery are also contemplated. Parenteral administration, such as intravenous, intramuscular, subcutaneous or other means of administration of interleukin-12 protein, concurrently with or subsequent to parenteral introduction of the vaccine compositions of this invention is also advantageous.
[0046] The following non-limiting Examples are presented to better illustrate the workings of the invention.
EXAMPLE 1
[0047] Construction of pAd34ΔE1ΔE4Ad5Orf6
[0048] To generate an E1-Ad34 based vector that can propagate in existing group C/Ad5 E1 complementing cell lines (293, PER.C6), Ad5 Orf6 was inserted in place of the native E4 region. To construct the Ad34 pre-Adenovirus plasmid, advantage was taken of the sequence homology between Ad34 and Ad35. Cotransformation of BJ 5183 bacteria with purified wild-type Ad34 viral DNA and the appropriately constructed Ad35 ITR cassette resulted in the circularization of the viral genome by homologous recombination. The construction of the pre-Ad plasmid based on Ad34, is outlined below:
[0049] To construct pAd34ΔE1ΔE4Ad5Orf6 (An Ad34 pre-Ad plasmid containing an E1 deletion and an E4 deletion substituted with Ad5 Orf6), we utilized an Ad35 ITR cassette. We anticipated that sequence homology between Ad34 and Ad35 would allow homologous recombination to occur. The Ad35 ITR cassette was constructed containing sequences from the right (bp 31599 to 31913 and bp 34419 to 34793) and left (bp 4 to 456 and bp 3403 to 3886) end of the Ad35 genome (see FIGS. 11A-1 to 11 A- 10 ) separated by plasmid sequences containing a bacterial origin of replication and an ampicillin resistance gene. The four segments were generated by PCR and cloned sequentially into pNEB193, generating pNEBAd35-4. Next the Ad5 Orf6 open reading frame was generated by PCR and cloned between Ad35 bp 31913 and 34419 generating pNEBAd35-4Ad5Orf6 (the ITR cassette). PNEB193 is a commonly used commercially available cloning plasmid (New England Biolabs cat# N3051S) containing a bacterial origin of replication, ampicillin resistance gene and a multiple cloning site into which the PCR products were introduced. The ITR cassette contains a deletion of E1 sequences from Ad35 bp 457 to 3402 with a unique Swa I restriction site located in the deletion and an E4 deletion from Ad35 bp 31914 to 34418 into which Ad5 Orf6 was introduced in an E4 parallel orientation. In this construct Ad5Orf6 expression is driven by the Ad35 E4 promoter. The Ad35 sequences (bp 31599 to 31913 and bp 3403 to 3886) in the ITR cassette provided regions of homology with the purified Ad34 viral DNA in which bacterial recombination could occur following cotransformation into BJ 5183 bacteria (FIG. 1). The ITR cassette was also designed to contain unique restriction enzyme sites (PmeI) located at the end of the viral ITR's so that digestion would release the recombinant Ad34 genome from the plasmid sequences. Potential clones were screened by restriction analysis and one clone was selected as pAd34ΔE1ΔE4Ad5Orf6.
EXAMPLE 2
[0050] Rescue of pAd34ΔE1ΔE4Ad5Orf6 into Virus
[0051] In order to determine if pre-adenovirus plasmid pAd34ΔE1ΔE4Ad5Orf6 could be rescued into virus and propagated in a group C E1 complementing cell line, the plasmid was digested with Pme I and transfected into T-25 flasks of PER.C6 cells using the calcium phosphate co-precipitation technique (Cell Phect Transfection Kit, Amersham Pharmacia Biotech Inc). PmeI digestion releases the viral genome from plasmid sequences allowing viral replication to occur after cell entry. Viral cytopathic effect (CPE), indicating that virus replication and amplification was occurring was observed following transfection. When CPE was complete, approximately 7-10 days post transfection, the infected cells and media were harvested, freeze/thawed three times and the cell debris pelleted by centrifugation. Approximately 1 ml of the cell lysate was used to infect a T-225 flask of PER.C6 cells at 80-90% confluence. Once CPE was reached, infected cells and media were harvested, freeze/thawed three times and the cell debris pelleted by centrifugation. Clarified cell lysates were then used to infect 2-layer NUNC cell factories of PER.C6 cells. Following complete CPE, the virus was purified by ultracentrifugation on CsCl density gradients. In order to verify the genetic structure of the rescued viruses, viral DNA was extracted using pronase treatment followed by phenol chloroform extraction and ethanol precipitation. Viral DNA was then digested with HindIII and treated with Klenow fragment to end-label the restriction fragments with P33-dATP. The end-labeled restriction fragments were then size-fractionated by gel electrophoresis and visualized by autoradiography. The digestion products were compared with the digestion products of the corresponding pre-Adenovirus plasmid (that had been digested with Pme1/HindIII prior to labeling) from which they were derived. The expected sizes were observed, indicating that the viruses had been successfully rescued.
EXAMPLE 3
[0052] Insertion of an Expression Cassette into pAd34ΔE1ΔE4Ad5Orf6
[0053] In order to introduce a gag or SEAP expression cassette (see FIGS. 8 and 9, respectively) into the E1 region of pAd34ΔE1ΔE4Ad5Orf6 bacterial recombination was again used. A gag expression cassette consisting of the following: 1) the immediate early gene promoter from human cytomegalovirus, 2) the coding sequence of the human immunodeficiency virus type 1 (HIV-1) gag (strain CAM-1; 1526 bp) gene, and 3) the bovine growth hormone polyadenylation signal sequence, was cloned into the E1 deletion in Ad35 shuttle plasmid, pNEBAd35-2 (a precursor to the Ad35 ITR cassettes described above), generating pNEBAd35CMVgagBGHpA. pNEBAd35-2 contains Ad35 sequences from the left end of the genome (bp 4 to 456 and bp 3403 to 3886) with a unique SwaI site between bp 456 and 3403 at the position of the deletion. The gag expression cassette was obtained from a previously constructed shuttle plasmid by EcoRI digestion. Following the digestion the desired fragment was gel purified, treated with Klenow to obtain blunt ends and cloned into the SwaI site in pNEBAd35-2. This cloning step resulted in the gag expression cassette being inserted into the E1 deletion between bp 456 and 3403 in the E1 parallel orientation. The shuttle vector containing the gag transgene was digested to generate a DNA fragment consisting of the gag expression cassette flanked by Ad35 bp 4 to 456 and bp 3403 to 3886 and the fragment was purified after electrophoresis on an agarose gel. Cotransformation of BJ 5183 bacteria with the shuttle vector fragment and pAd34ΔE1ΔE4Ad5Orf6 linearized in the E1 region by digestion with Swa I, resulted in the generation of the Ad34 gag-containing pre-Adenovirus plasmid pAd34ΔE1ΔE4Ad5Orf6 by homologous recombination. Potential clones were screened by restriction analysis.
[0054] A similar strategy was used to generate Ad34 pre-Ad plasmids containing a SEAP expression cassette. In this case a SEAP expression cassette consisting of: 1) the immediate early gene promoter from human cytomegalovirus, 2) the coding sequence of the human placental SEAP gene, and 3) the bovine growth hormone polyadenylation signal sequence was cloned into the E1 deletion in Ad35 shuttle plasmid, pNEBAd35-2, generating pNEBAd35CMVSEAPBGHpA. The SEAP expression cassette was obtained from a previously constructed shuttle plasmid by EcoRI digestion. Following the digestion the desired fragment was gel purified, treated with Klenow to obtain blunt ends and cloned into the SwaI site in pNEBAd35-2. The transgene was then recombined into the pAd34ΔE1ΔE4Ad5Orf6, generating pAd34ΔE1ΔE4Ad5Orf6 as described above for the gag transgene.
[0055] All pre-Ad plasmids were rescued into virus and expanded to prepare CsCl purified stocks as described above.
EXAMPLE 4
[0056] Construction of pMRKAd34ΔE1ΔE4Ad5Orf6
[0057] To construct an Ad34 pre-Ad plasmid that was composed entirely of Ad34 sequences, an Ad34 ITR cassette was generated. The Ad34 ITR cassette was constructed containing sequences from the right (bp 31584 to 31895 and bp 34409 to 34772) and left (bp 4 to 456 and bp 3402 to 3885) end of the Ad34 genome (see FIGS. 3A-1 to 3 A- 9 ) separated by plasmid sequences containing a bacterial origin of replication and an ampicillin resistance gene. These four segments were generated by PCR and cloned sequentially into pNEB193, generating pNEBAd34-4. Next the Ad5 Orf6 open reading frame was generated by PCR and cloned between Ad34 bp 31895 and 34409 generating pNEBAd34-4Ad5Orf6 (the ITR cassette). PNEB193 is a commonly used commercially available cloning plasmid (New England Biolabs cat# N3051S) containing a bacterial origin of replication, ampicillin resistance gene and a multiple cloning site into which the PCR products were introduced. The ITR cassette contains a deletion of E1 sequences from Ad34 bp 457 to 3401 with a unique Swa I restriction site located in the deletion and an E4 deletion from Ad34 bp 31896 to 34408 into which Ad5 Orf6 was introduced in an E4 parallel orientation. In this construct Ad5Orf6 expression is driven by the Ad34 E4 promoter. The Ad34 sequences (bp 31584 to 31895 and bp 3402 to 3885) in the ITR cassette provided regions of homology with the purified Ad34 viral DNA in which bacterial recombination could occur following cotransformation into BJ 5183 bacteria (FIG. 2). The ITR cassette was also designed to contain unique restriction enzyme sites (PmeI) located at the end of the viral ITR's so that digestion would release the recombinant Ad34 genome from the plasmid sequences. Potential clones were screened by restriction analysis and one clone was selected as pMRKAd34ΔE1ΔE4Ad5Orf6.
EXAMPLE 5
[0058] In Vivo Studies
[0059] A. Immunization
[0060] Cohorts of 3 rhesus macaques were given single intramuscular injections of one of the two vectors: (1) 10{circumflex over ( )}11 vp MRKAd5-SEAP (in MRKAd vector backbone of FIGS. 10A-1 to 10 A- 45 disclosed in PCT/US01/28861, published Mar. 21, 2002); and (2) 10{circumflex over ( )}11 vp Ad34ΔE1SEAPΔE4Ad5Orf6. Rhesus macaques were between 3-10 kg in weight. In all cases, the total dose of each vaccine was suspended in 1 mL of buffer. The macaques were anesthetized (ketamine/xylazine) and the vaccines were delivered i.m. in 0.5-mL aliquots into both deltoid muscles using tuberculin syringes (Becton-Dickinson, Franklin Lakes, N.J.). Peripheral blood mononuclear cells (PBMC) were prepared from blood samples collected at several time points during the immunization regimen. All animal care and treatment were in accordance with standards approved by the Institutional Animal Care and Use Committee according to the principles set forth in the Guidefor Care and Use of Laboratory Animals , Institute of Laboratory Animal Resources, National Research Council.
[0061] B. SEAP Assay
[0062] Serum samples were analyzed for circulating human secreted alkaline phosphatase (SEAP) levels using TROPIX phospha-light chemiluminescent kit (Applied Biosystems Inc). Duplicate 5 μL aliquots of each serum were mixed with 45 μL of kit-supplied dilution buffer in a 96-well white DYNEX plate. Serially diluted solutions of a human placental alkaline phosphatase (Catalog no. M5905, Sigma, St. Louis, Mo.) in 10% naive monkey serum served to provide the standard curve. Endogenous SEAP activity in the samples was inactivated by heating the well for 30 minutes at 65° C. Enzymatic SEAP activities in the samples were determined following the procedures described in the kit. Chemiluminescence readings (in relative light units) were recorded using DYNEX luminometer. RLU readings were converted to ng/mL SEAP using a log-log regression analyses.
[0063] C. ELISPOT Assay
[0064] The IFN-γ ELISPOT assays for rhesus macaques were conducted following a previously described protocol (Allen et al., 2001 J. Virol. 75(2):738-749), with some modifications. For antigen-specific stimulation, a peptide pool was prepared from 20-aa peptides that encompass the entire HIV-1 gag sequence with 10-aa overlaps (Synpep Corp., Dublin, Calif.). To each well, 50 μL of 2-4×10 5 peripheral blood mononuclear cells (PBMCs) were added; the cells were counted using Beckman Coulter Z2 particle analyzer with a lower size cut-off set at 80 femtoliters (“fL”). Either 50 μL of media or the gag peptide pool at 8 μg/mL concentration per peptide were added to the PBMC. The samples were incubated at 37° C., 5% CO 2 for 20-24 hrs. Spots were developed accordingly and the plates were processed using custom-built imager and automatic counting subroutine based on the ImagePro platform (Silver Spring, Md.); the counts were normalized to 10 6 cell input.
[0065] D. Intracellular Cytokine Staining (ICS)
[0066] To 1 ml of 2×10 6 PBMC/mL in complete RPMI media (in 17×100 mm round bottom polypropylene tubes (Sarstedt, Newton, N.C.)), anti-hCD28 (clone L293, Becton-Dickinson) and anti-hCD49d (clone L25, Becton-Dickinson) monoclonal antibodies were added to a final concentration of 1 μg/mL. For gag-specific stimulation, 10 μL of the peptide pool (at 0.4 mg/mL per peptide) were added. The tubes were incubated at 37° C. for 1 hr., after which 20 μL of 5 mg/mL of brefeldin A (Sigma) were added. The cells were incubated for 16 hr at 37° C., 5% CO 2 , 90% humidity. 4 mL cold PBS/2% FBS were added to each tube and the cells were pelleted for 10 min at 1200 rpm. The cells were re-suspended in PBS/2% FBS and stained (30 min, 4° C.) for surface markers using several fluorescent-tagged mAbs: 20 μL per tube anti-hCD3-APC, clone FN-18 (Biosource); 20 μL anti-hCD8-PerCP, clone SK1 (Becton Dickinson); and 20 μL anti-hCD4-PE, clone SK3 (Becton Dickinson). Sample handling from this stage was conducted in the dark. The cells were washed and incubated in 750 μL 1×FACS Perm buffer (Becton Dickinson) for 10 min at room temperature. The cells were pelleted and re-suspended in PBS/2% FBS and 0.1 μg of FITC-anti-hIFN-γ, clone MD-1 (Biosource) was added. After 30 min incubation, the cells were washed and re-suspended in PBS. Samples were analyzed using all four color channels of the Becton Dickinson FACSCalibur instrument. To analyze the data, the low side- and forward-scatter lymphocyte population was initially gated; a common fluorescence cut-off for cytokine-positive events was used for both CD4 + and CD8 + populations, and for both mock and gag-peptide reaction tubes of a sample.
[0067] E. Results
[0068] Expression: Serum samples prior to and after the injection were analyzed for circulating SEAP activities and the results are shown in FIG. 4. Results indicate that the peak levels of SEAP protein produced by the alternative adenovirus serotype were lower than but were within 3-fold of that of MRKAd5 at the same high dose level of 10{circumflex over ( )}11 vp (FIG. 4). The levels of SEAP in the serum dropped dramatically after day 10 and were close to background as early as day 15. These observations strongly indicate that the Ad34-based vector is efficient in expressing a transgene following intramuscular administration in a primate.
[0069] Immunogenicity: Vaccine-induced T cell responses against HIV-1 gag were quantified using IFN-gamma ELISPOT assay against a pool of 20-aa peptides that encompassed the entire protein sequence. The results are shown in FIG. 5; they are expressed as the number of spot-forming cells (SFC) per million peripheral blood mononuclear cells (PBMCs) that responded to the peptide pool or the mock (no peptide) control.
[0070] Immunization with gag-expressing Ad34 vector induced detectable levels of circulating gag-specific T cells immediately after a single dose of the vector. The responses improved following a second dose given at wk 4. Overall, the responses to the Ad34-based vector were slightly lower than those induced by the same dose of MRKAd5-gag. The results strongly indicate the Ad34-based vector can prime effectively for HIV-specific T cell responses.
[0071] IFN-γ ICS analyses of the PBMC from the Ad34-immunized animals revealed that the vector can induce detectable levels of both CD4 + and CD8 + HIV-specific T cells (FIG. 6).
EXAMPLE 6
[0072] Heterologous Immunization
[0073] Cohorts of 3 monkeys were immunized (at wks 0, 4) with 10{circumflex over ( )}11 vp Ad34ΔE1gagΔE4Ad5Orf6 followed by a booster at week 24 with 10{circumflex over ( )}10 vp Ad35ΔE1gagΔE4Ad5Orf6. Vaccine-induced T cell responses against HIV-1 gag were quantified using IFN-gamma ELISPOT assay against a pool of 20-aa peptides that encompassed the entire protein sequence. The results are shown in FIG. 12; they are expressed as the number of spot-forming cells (SFC) per million peripheral blood mononuclear cells (PBMCs) that responded to the peptide pool or the mock (no peptide) control.
[0074] Immunization with gag-expressing Ad34 vector induced detectable levels of circulating gag-specific T cells that decreased to between 94-139 SFC/10{circumflex over ( )}6 PBMC at the time of the boost. Heterologous immunization with an Ad35-based HIV vector resulted in as much as a 3-fold increase in T cell responses.
[0075] IFN- 65 ICS analyses of the PBMCs from the Ad34 primed/Ad35 boosted animals at week 28 revealed that the vector can induce detectable levels of both CD4 + and CD8 + HIV-specific T cells (FIG. 13).



1


13


1
34775
DNA
adenovirus serotype 34

1

catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtgatttta 60

aaaattgtgg ggtgtgtggt gattggctgt ggggttaacg gctaaacggg gcggcgcggc 120

cgtgggaaaa tgacgttttg tgggggtgga gtttttttgc aagttgtcgc gggaaatgtg 180

acgcataaaa aggctttttt tctcacggaa ctactgactt ttcccacggt atttaacagg 240

aaatgaggta gttttgaccg gatgcaagtg aaaattgctg atttgcgcgc gaaaactgaa 300

tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360

ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420

ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctacggtatt 480

tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540

tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600

aatttctgct gagactggaa atgaaatact ggagcttgtg gtgcacgccc tgatgggaga 660

cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720

agaggtagag ggatcggagg attctaatga ggaagctgtg aatggctttt ttaccgattc 780

tatgctttta gctgctaatg aaggattaga attagatccg cctttggaca ctttcgatac 840

tccaggggtg attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgggttccgt 900

ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960

aaaggagcag tctatgcaga ctgcagcggg tgagggagtg aaggctgcca gtgttggttt 1020

tcagttggat tgcccggagc ttcctggaca tggctgtaag tcttgtgaat ttcacaggaa 1080

aaatactgga gtaaaggaac tgttatgttc gctttgttat atgagagcgc actgccactt 1140

tatttacagt aagtgtgttt aagttaaaat ttaaaggaat atgctgtttt tcacatgtat 1200

attgagtggg agttttgtgc ttcttattat aggtcctgtg tctgatgctg atgagtcacc 1260

atctcctgat tctactacct cacctcctga gattcaagca cctgttcctg tggacgtgcg 1320

caagcccatt cctgtgaagc ttaagcctgg gaaacgtcca gcagtggaaa aacttgagga 1380

cttgttacag ggtggggacg gacctttgga cttgagtaca cggaaacggc caagacaata 1440

agtgttccat atccgtgttt acttaaggtg acgtcaatat ttgtgtgaga gtgcaatgta 1500

ataaaaatat gttaactgtt cactggtttt tattgctttt tgggcgggga ctcaggtata 1560

taagtagaag cagacctgta tggttagctc ataggagctg gctttcatcc atggaggttt 1620

gggccatttt ggaagacctt agaaagacta ggcaactgtt agaggacgct tcggacggag 1680

tctccggttt ttggagattc tggttcgcta gtgaattagc tagggtagtt tttaggataa 1740

aacaggacta taaagaagaa tttgaaaagt tgttggtaga ttgcccagga ctttttgaag 1800

ctcttaattt gggccatcaa gttcacttta aagaaaaagt tttatcagtt ttagactttt 1860

caaccccagg tagaactgcc gctgctgtgg cttttcttac ttttatatta gataaatgga 1920

tcccgcagac tcatttcagc aggggatacg ttttggattt cgtagccaca gcattgtgga 1980

gaacatggaa ggttcgcaag atgaggacaa tcttaggtta ctggccagtg cagcctttgg 2040

gtgtagcggg aatcctgagg catccaccgg tcatgccagc ggttctggag gaggaacagc 2100

aagaggacaa cccgagagcc ggcctggacc ctccagtgga ggaggcggag tagctgactt 2160

gtctcctgaa ctgcaacggg tgcttactgg atctacgtcc actggacggg ataggggcgt 2220

taagagggag agggcatcta gtggtactga tgctagatct gagttggctt taagtttaat 2280

gagtcgcaga cgtcctgaaa ccatttggtg gcatgaggtc cagaaagagg gaagggatga 2340

agtttctgta ttgcaggaga aatattcact ggaacaggtg aaaacatgtt ggttggagcc 2400

tgaggatgat tgggaggtgg ccattaaaaa ttatgccaag atagctttga ggcctgataa 2460

acagtataag attactagac ggattaatat ccggaatgct tgttacatat ctggaaatgg 2520

ggctgaggtg gtaatagata ctcaagacaa ggcagttatt agatgctgca tgatggatat 2580

gtggcctgga gtagtcggta tggaagcagt aacttttgta aatgttaagt ttaggggaga 2640

tggttataat ggaatagtgt ttatggccaa taccaaactt atattgcatg gttgtagctt 2700

ttttggtttc aacaatacct gtgtagatgc ctggggacag gttagtgtac ggggatgtag 2760

tttctatgcg tgttggattg ccacagctgg cagaaccaag agtcaattgt ctctgaagaa 2820

atgcatattc caaagatgta acctgggcat tctgaatgaa ggcgaagcaa gggtccgcca 2880

ctgcgcttct acagatactg gatgttttat tttaattaag ggcaatgcca gcgtaaagca 2940

taacatgatt tgcggtgctt ccgatgagag gccttatcaa atgctcactt gtgccggtgg 3000

gcattgtaat atgctggcta ctgtgcatat tgtttcccat caacgcaaaa aatggcctgt 3060

ttttgatcac aatgtgttga ccaagtgtac catgcatgca ggtgggcgta gaggaatgtt 3120

tatgccttac cagtgtaaca tgaatcatgt gaaagtgttg ttggaaccag atgccttttc 3180

cagaatgagc ctaacaggaa tctttgacat gaacatgcaa atctggaaga tcctgaggta 3240

tgatgatacg agatcgaggg tgcgcgcatg cgaatgcgga ggcaagcatg ccaggttcca 3300

gccggtgtgt gtagatgtga ctgaagatct gagaccggat catttggtta ttgcccgcac 3360

tggagcagag ttcggatcca gtggagaaga aactgactaa ggtgagtatt gggaaaactt 3420

ggggtggggt tttcagatgg acagattgag taaaaatttg ttttttctgt ctttcagctg 3480

tcatgagtgg aaacgcttct tttaaggggg gagtcttcag cccttatctg acagggcgtc 3540

tcccatcctg ggcaggagtt cgtcagaatg ttatgggatc tactgtggat ggaagacccg 3600

tccaacccgc caattcttca acgctgacct atgctacttt aagttcttca cctttggacg 3660

cagctgcagc cgccgccgcc gcctctgttg ccgctaacac tgtgcttgga atgggttact 3720

atggaagtat cgtggctaat tccacttcct ctaataaccc ttctaccctg actcaggaca 3780

agttacttgt ccttttggcc cagctggagg ctttgaccca acgtctgggt gaactttatc 3840

agcaggtggc cgagttgcga gtacaaactg agtctgctgt cggcacggca aagtctaaat 3900

aaaaaaaaat tccacaatca atgaataaat aaacgagctt gttgttgatt taaaatcaag 3960

tgtttttatt tcatttttcg cgcacggtat gccctagacc accgatctcg atcattgaga 4020

acacggtgga ttttttccag aatcctatag aggtgggatt gaatgtttag atacatgggc 4080

attaggccat ctttggggtg gagatagctc cattgaaggg attcatgctc cggggtagtg 4140

ttgtaaatca cccagtcata acaaggtcgc agtgcatggt gttgcacaat atcttttaga 4200

agtaggctga ttgccacaga taagcccttg gtgtaggtgt ttacaaaccg gttgagctgg 4260

gaggggtgca ttcggggtga aattatgtgc attttggatt ggatttttaa gttggcaata 4320

ttgccgccaa gatctcgtct tgggttcatg ttatgaagga ccaccaagac ggtgtatccg 4380

gtacatttag gaaatttatc gtgtagcttg gatggaaaag cgtggaaaaa tttggagaca 4440

cccttgtgtc ctccgagatt ttccatgcac tcatccatga taatagcaat ggggccgtgg 4500

gcagcagcgc gggcaaacac gttccgtggg tctgacacat catagttatg ttcctgagtt 4560

aaatcatcat aagccatttt aatgaatttg gggcggagag tacccgattg gggtatgaat 4620

gttccttcgg gccccggagc atagttcccc tcacagattt gcatttccca agctttcagt 4680

tccgatggtg gaatcatgtc cacctggggg gctatgaaga acaccgtttc tggggcgggg 4740

gtgattagtt gggatgatag caagtttctg agcaattgag atttgccaca tccggtgggg 4800

ccataaatga ttccgattac aggttgcagg tggtagttta gggaacggca actgccgtct 4860

tctcgaagca agggggccac ctcgttcatc atttccctta catgcatatt ttcccgcacc 4920

aaatccatta ggaggcgctc tcctcctagt gatagaagtt cttgtagtga ggaaaagttt 4980

ttcagcggtt ttagaccgtc agccatgggc attttggaga gagtttgctg caaaagttct 5040

agtctgttcc acagttcagt gatgtgttct atggcatctc gatccagcag acctcctcgt 5100

ttcgcgggtt tggacggctc ctggagtagg gtatgagacg atgggcgtcc agcgctgcca 5160

gggttcggtc cttccagggt ctcagtgttc gagtcagggt tgtttccgtc acagtgaagg 5220

ggtgtgcgcc tgcttgggcg cttgccaggg tgcgcttcag actcattctg ctggtggaga 5280

acttctgtcg cttggcgccc tgtatgtcgg ccaagtagca gtttaccatg agttcgtagt 5340

tgagcgcctc ggctgcgtgg cctttggcgc ggagcttacc tttggaagtt ttcttgcata 5400

ccgggcagta taggcatttc agcgcataca gcttgggcgc aaggaaaatg gattctgggg 5460

agtatgcatc tgcgccgcag gaggcgcaaa cagtttcaca ttccaccagc caggttaaat 5520

ccggttcatt ggggtcaaaa acaagttttc cgccatattt tttgatgcgt ttcttacctt 5580

tggtctccat gagttcgtgt cctcgttgag tgacaaacag gctgtccgta tccccgtaga 5640

ctgattttac aggcctcttc tccagtggag tgcctcggtc ttcttcgtac aggaactctg 5700

accactctga tacaaaggcg cgcgtccagg ccagcacaaa ggaggctatg tgggaggggt 5760

agcgatcgtt gtcaaccagg gggtccacct tttccaaagt atgcaaacac atgtcaccct 5820

cttcaacatc caggaatgtg attggcttgt aggtgtattt cacgtgacct ggggtccccg 5880

ctgggggggt ataaaagggg gcggttcttt gctcttcctc actgtcttcc ggatcgctgt 5940

ccaggaacgt cagctgttgg ggtaggtatt ccctctcgaa ggcgggcatg acctctgcac 6000

tcaggttgtc agtttctaag aacgaggagg atttgatatt gacagtgccg gttgagatgc 6060

ctttcatgag gttttcgtcc atttggtcag aaaacacaat ttttttattg tcaagtttgg 6120

tggcaaatga tccatacagg gcgttggata aaagtttggc aatggatcgc atggtttggt 6180

tcttttcctt gtccgcgcgc tctttggcag cgatgttgag ttggacatac tcgcgtgcta 6240

ggcacttcca ttcggggaag atagttgtca attcatctgg cacgattctc acttgccacc 6300

ctcgattatg caaggtaatt aaatccacac tggtggccac ctcgcctcga aggggttcgt 6360

tggtccaaca gagcctacct cctttcctag aacagaaagg gggaagtggg tctagcataa 6420

gttcatcggg agggtctgca tccatggtaa agattcccgg aagtaaatcc ttatcaaaat 6480

agctgatggg agtggggtca tctaaggcca tttgccattc tcgagctgcc agtgcacgct 6540

catatgggtt aaggggactg ccccagggca tgggatgggt gagtgcagag gcatacatgc 6600

cacagatgtc atagacgtag atgggatcct caaagatgcc tatataggtt ggatagcatc 6660

gcccccctct gatacttgct cgcacatagt catatagttc atgtgatggc gctagcaacc 6720

ccggacccaa gttggtgcga ttgggttttt ctgttctgta gacaatctgg cgaaagatgg 6780

cgtgagaatt ggaagagatg gtgggtcttt gaaaaatgtt gaaatgggca tgaggtagac 6840

ctacagagtc tctgacaaag tgggcataag attcttgaag cttggttacc agttcggcgg 6900

tgacaagtac gtctagggcg cagtagtcaa gtgtttcttg aatgatgtca taacctggtt 6960

ggtttttctt ttcccacagt tcgcggttga gaaggtattc ttcgcgatcc ttccagtact 7020

cttctagcgg aaacccgtct ttgtctgcac ggtaagatcc tagcatgtag aactgattaa 7080

ctgccttgta agggcagcag cccttctcta cgggtagaga gtatgcttga gcagcttttc 7140

gcagcgaagc gtgagtaagg gcgaaggtgt ctctgaccat gactttgaga aattggtatt 7200

tgaagtccat gtcgtcacag gctccctgtt cccagagttg gaagtctacc cgtttcttgt 7260

aggcggggtt gggcaaagcg aaagtaacat cgttgaagag aatcttaccg gctctgggca 7320

taaaattgcg agtgatgcgg aaaggctgtg gtacttccgc tcgattgttg atcacctggg 7380

cagctaggac gatctcgtcg aaaccgttga tgttgtgtcc tacgatgtat aattctatga 7440

aacgcggcgt gcctttgacg tgaggtagct tattgagctc atcaaaggtt aggtctgtag 7500

ggtcagataa ggcgtagtgt tcgagagccc attcgtgcag gtgaggattt gcatgtagga 7560

atgatgacca aagatccacc gccagtgctg tttgtaactg gtcccgatac tgacgaaaat 7620

gctggccaat tgccattttt tctggagtga cacagtagaa ggttctgggg tcttgttgcc 7680

atcgatccca ctttagttta atggctagat cgtgggccat gttgacgaga cgctcttctc 7740

ctgagagttt catgaccagc atgaaaggaa ctagttgttt gccaaaggac cccatccagg 7800

tgtaagtttc cacatcgtag gtcaggaaga gtctttctgt gcgaggatga gagccgatcg 7860

ggaagaactg gatttcctgc caccagttgg aggattggct gttgatgtga tggaagtaga 7920

agtttctgcg gcgcgccgag cattcgtgtt tgtgcttgta cagacggccg cagtagtcgc 7980

agcgttgcac gggttgtatc tcgtgaatga gctgtacctg gcttcccttg acgagaaatt 8040

tcagtgggaa gccgaggcct ggcgattgta tctcgtgctc ttctatattc gctgtatcgg 8100

cctgttcatc ttctgtttcg gtggtggtca tgctgacgag cccccgcggg aggcaagtcc 8160

agacctcggc gcgggagggg cggagctgaa ggaccagagc gcgcaggctg gagctgtcca 8220

gagtcctgag acgctgcgga ctcaggttag taggtaggga cagaagatta acttgcatga 8280

tcttttccag ggcgtgcggg aggttcagat ggtacttgat ttccacaggt tcgtttgtag 8340

agatgtcaat ggcttgcagg gttccgtgtc ctttgggcgc cactaccgta cctttgtttt 8400

ttcttttgat cggtggtggc tctcttgctt cttgcatgct cagaagcgat gacggggacg 8460

cgcgccgggc ggaagcggtt gttccggacc cggaggcatg gctggtagtg gcacgtcggc 8520

gccgcgcacg ggcaggttct ggtactgcgc tctgagaaga cttgcgtgcg ccaccacgcg 8580

tcgattgacg tcttgtatct gacgtctctg ggtgaaagct accggccccg tgagcttgaa 8640

cctgaaagag agttcaacag aatcaatttc ggtatcgtta acggcagctt gtctcagtat 8700

ttcttgtacg tcaccagagt tgtcctggta ggcgatctcc gccatgaact gctcgatttc 8760

ttcctcctga agatctccgc gacccgctct ctcgacggtg gccgcgaggt cattggagat 8820

acggcccatg agttgggaga atgcagtcat gcccgcctcg ttccagacgc ggctgtaaac 8880

cacggccccc tcggagtctc ttgcgcgcat caccacctga gcgaggttaa gctccacgtg 8940

tctggtgaag accgcatagt tgcataggcg ctgaaaaagg tagttgagtg tggtggcaat 9000

gtgttcggcg acgaagaaat acatgatcca tcgtctcagc ggcatttcgc tgacatcgcc 9060

cagagcttcc aagcgctcca tggcctcgta gaagtccacg gcaaaattaa aaaactggga 9120

gtttcgcgcg gacacggtca attcctcctc gagaagacgg atgagttcgg ctatggtggc 9180

ccgtacttcg cgttcgaagg ctcccgggat ctcttcttcc tcttctatct cttcttccac 9240

taacatctct tcttcgtctt caggcggggg cggagggggc acacggcgac gtcgacggcg 9300

cacgggcaaa cggtcgatga atcgttcaat gacctctccg cggcggcggc gcatggtttc 9360

agtgacggcg cggccgttct cgcgcggtcg cagagtaaaa acaccgccgc gcatctcctt 9420

aaagtggtga ctgggaggtt ctccgtttgg gagggagagg gcgctgatta tacattttat 9480

taattggccc gtagggactg cgcgcagaga tctgatcgtg tcaagatcca cgggatctga 9540

aaacctttcg acgaaagcgt ctaaccagtc acagtcacaa ggtaggctga gtacggcttc 9600

ttgtgggcgg gggtggttat gtgttcggtc tgggtcttct gtttcttctt catctcggga 9660

aggtgagacg atgctgctgg tgatgaaatt aaagtaggca gttctaagac ggcggatggt 9720

ggcgaggagc accaggtctt tgggtccggc ttgctggata cgcaggcgat tggccattcc 9780

ccaagcatta tcctgacatc tagcaagatc tttgtagtag tcttgcatga gccgttctac 9840

gggcacttct tcctcacccg ttctgccatg catacgtgtg agtccaaacc cgcgcattgg 9900

ttgtaccagt gccaagtcag ctacgactct ttcggcgagg atggcttgct gtacttgggt 9960

gagggtggct tgaaagtcat caaaatccac aaagcggtgg taagccccgg tattaatggt 10020

gtaagcacag ttggccatga ctgaccagtt aactgtctgg tgaccagggc gcacgagctc 10080

ggtgtattta aggcgcgaat aggcgcgggt gtcaaagatg taatcgttgc aggtgcgcac 10140

cagatactgg taacctataa gaaaatgcgg cggtggttgg cggtagagag gccatcgttc 10200

tgtagctgga gcgccggggg cgaggtcttc caacataagg cggtgatagc cgtagatgta 10260

cctggacatc caggtgattc ctgcggcggt agtagaagcc cgaggaaact cgcgtacgcg 10320

gttccaaatg ttgcgtagcg gcatgaagta gttcattgta ggcacggttt gaccagtgag 10380

gcgcgcgcag tcattgatgc tctatagaca cggagaaaat gaaagcgttc agcgactcga 10440

ctccgtagcc tggaggaacg tgaacgggtt gggtcgcggt gtaccccggt tcgagacttg 10500

tactcgagcc ggccggagcc gcggctaacg tggtattggc actcccgtct cgacccagcc 10560

tacaaaaatc caggatacgg aatcgagtcg ttttgctggt tgccgaatgg cagggaagtg 10620

agtcctattt tttttttttg ccgctcagat gcatcccgtg ctgcgacaga tgcgtcccca 10680

acaacagccc ccctcgcagc agcagcaacc acaaaaggct gtccctgcaa ctactgcaac 10740

tgccgctgtg agcggtgcgg gacagcccgc ctatgatctg gacttggaag agggcgaagg 10800

actggcacgt ctaggtgcgc cttcgcccga gcggcatccg cgagttcaac tgaaaaaaga 10860

ttctcgcgag gcgtatgtgc cccaacagaa cctatttaga gacagaagcg gcgaggagcc 10920

ggaggagatg cgagcttccc gctttaacgc gggtcgtgag ctgcgtcacg gtttggacag 10980

aagacgagtg ttgcgggacg aggatttcga agttgatgaa gtgacaggga tcagtcctgc 11040

cagggcacac gtggctgcag ccaaccttgt atcggcttac gaacagacag taaaggaaga 11100

gcgtaatttc caaaagtctt ttaataatca tgtgcgaacc ctcattgccc gcgaagaagt 11160

cacccttggt ttgatgcatt tgtgggattt gatggaagct atcattcaga accctactag 11220

caaacctctg accgcacagc tgtttctggt ggtgcaacac agcagagaca atgaggcttt 11280

cagagaggcg ctgctcaaca tcaccgaacc cgaggggaga tggttgtatg atcttatcaa 11340

cattctacag agtatcatag tgcaggagcg gagcctgggc ctggccgaga aggtggctgc 11400

catcaattac tcggttttga gcttgggaaa gtattacgct cgcaagatct acaagactcc 11460

atacgttccc atagacaagg aggtgaagat agatgggttc tacatgcgca tgacgctgaa 11520

ggtgttgacc ctgagcgatg atcttggggt gtaccgcaat gacagaatgc atcgcgcggt 11580

gagcgccagc aggaggcgcg agttaagcga cagggaactg atgcacagtt tgcaaagagc 11640

tctaactgga gctggaaccg agggtgagaa ttactttgat atgggagctg acttgcagtg 11700

gcagcctagt cgcagggctc tgaacgccgc gacggcagga tgtgagcttc cttacataga 11760

agaggcggat gaaggcgagg aggaagaggg cgagtacttg gaagactgat ggcacaaccc 11820

gtgttttttg ctagatggaa cagcaagcac cggatcccgc aatgcgggcg gcgctgcaga 11880

gccagccgtc cggcattaac tcctcggacg attggaccca ggccatgcaa cgtatcatgg 11940

cgttgacgac tcgcaacccc gaagccttta gacagcaacc ccaggccaac cgtctatcgg 12000

ccatcatgga agctgtagtg ccttcccgct ctaatcccac tcatgagaag gtcctggcca 12060

tcgtgaacgc gttggtggag aacaaagcta ttcgtccaga tgaggccgga ctggtataca 12120

acgctctctt agaacgcgtg gctcgctaca acagtagcaa tgtgcaaacc aatttggacc 12180

gtatgataac agatgtacgc gaagccgtgt ctcagcgcga aaggttccag cgcgatgcca 12240

acctgggttc gctggtggcg ttaaatgctt tcttgagtac tcagcctgct aatgtgccgc 12300

gtggtcaaca ggattatact aactttttaa gtgctttgag actgatggta tcagaagtac 12360

ctcagagcga agtatatcag tccggtcctg attacttctt tcagactagc agacagggct 12420

tgcagacggt aaatctgagc caagctttta aaaaccttaa aggtttgtgg ggagtgcatg 12480

ccccggtagg agaaagagca accgtgtcta gcttgttaac tccgaactcc cgcctattat 12540

tactgttggt agctcctttc accgacagcg gtagcatcga ccgtaattcc tatttgggtt 12600

acctactaaa cctgtatcgc gaagccatag ggcaaagtca ggtggacgag cagacctatc 12660

aagaaattac ccaagtcagt cgcgctttgg gacaggaaga cactggcagt ttggaagcca 12720

ctctgaactt cttgcttacc aatcggtctc aaaagatccc tcctcaatat gctcttactg 12780

cggaggagga gaggatcctt agatatgtgc agcagagcgt gggattgttt ctgatgcaag 12840

agggggcaac tccgactgca gcactggaca tgacagcgcg aaatatggag cccagcatgt 12900

atgccagtaa ccgacctttc attaacaaac tgctggacta cttgcacaga gctgccgcta 12960

tgaactctga ttatttcacc aatgccatct taaacccgca ctggctgccc ccacctggtt 13020

tctacacggg cgaatatgac atgcccgacc ctaatgacgg atttctgtgg gacgacgtgg 13080

acagcgatgt tttttcacct ctttctgatc atcgcacgtg gaaaaaggaa ggcggcgata 13140

gaatgcattc ttctgcatcg ctgtccgggg tcattggtgc taccgcggct gagcccgagt 13200

ctgcaagtcc ttttcctagt ctaccctttt ctctacacag tgtacgtagc agcgaagtgg 13260

gtagaataag tcgcccgagt ttaatgggcg aagaggagta cctaaacgat tccttgctca 13320

gaccggcaag agaaaaaaat ttcccaaaca atggaataga aagtttggtg gataaaatga 13380

gtagatggaa gacttatgct caggatcaca gagacgagcc tgggatcatg gggactacaa 13440

gtagagcgag ccgtagacgc cagcgccatg acagacagag gggtcttgtg tgggacgatg 13500

aggattcggc cgatgatagc agcgtattgg acttgggtgg gagaggaagg ggcaacccgt 13560

ttgctcattt gcgccctcgc ttgggtggta tgttgtaaaa aaaaataaaa aagaaaaaac 13620

tcaccaaggc catggcgacg agcgtacgtt cgttcttctt tattatctgt gtctagtata 13680

atgaggcgag tcgtgctagg cggagcggtg gtgtatccgg agggtcctcc tccttcgtac 13740

gagagcgtga tgcagcagca gcaggcgacg gcggtgatgc aatccccact ggaggctccc 13800

tttgtgcctc cgcgatacct ggcacctacg gagggcagaa acagcattcg ttactcggaa 13860

ctggcacctc agtacgatac caccaggttg tatctggtgg acaacaagtc ggcggacatt 13920

gcttctctga actatcagaa tgaccacagc aacttcttga ccacggtggt gcaaaacaat 13980

gactttaccc ctacggaagc cagcacccag accattaact ttgatgaacg atcgcggtgg 14040

ggcggtcagc taaaaaccat catgcatact aacatgccca acgtgaacga gtatatgttt 14100

agtaacaagt tcaaagcgcg tgtgatggtg tccagaaaac ctcctgaggg tgttagagta 14160

gacgataatt atgatcataa gcaagatatt ctaaaatacg agtggttcga gtttactttg 14220

ccagaaggca acttttcggt cactatgact atcgacttga tgaacaatgc catcatagac 14280

aattacttga aagtgggcag acagaatgga gtgttggaaa gtgacattgg tgttaagttc 14340

gacactagga acttcaagtt gggatgggat ccagaaacta agttgatcat gcctggggtt 14400

tacacctatg aggccttcca tcctgacatc gtattgctgc ctggctgcgg agtggacttt 14460

accgaaagcc gtctgagcaa ccttcttggc attagaaaga aacacccatt ccaagagggt 14520

tttaagatct tgtatgagga tttagaagga ggaaatattc cagccctttt ggatgtagat 14580

gcttatgaga acagcaagaa agatcaaaaa gccaaaatag aagctgctgc agaagctaaa 14640

gcaaacatag ttgccaacga tccggtaagg gtggctaacg ctagtgaaat caggggagac 14700

agttttgccg caacatccgt tccgactaaa gaatcattat tggatgatgt gtctcaaaac 14760

atagagttaa aactcactat taagcctgtg gaaaaagatg gcaaaaacag aagttacaat 14820

gtgttggaag ataaaatcaa cacggcctat cgcagttggt acctttcgta caattatggc 14880

gaccccgaaa aaggagtgcg ttcctggaca ttgctcacca cctcagatgt cacctgcgga 14940

gcggagcagg tctactggtc gcttccagac atgatgcagg atcctgtcac tttccgctcc 15000

actagacaag tcagtaacta ccctgtggtg ggtgcagagc ttatgcccgt cttttcaaag 15060

agcttctaca acgaacaagc tgtgtactcc cagcagctcc gccagtccac ctcgcttacg 15120

cacgtcttca accgctttcc tgagaaccag attttaatcc gtccgccggc gcccacaatt 15180

accaccgtca gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gttgcgcagc 15240

agtatccggg gagtccaacg tgtgaccgtt actgacgcca gacgccgcac ctgtccctac 15300

gtgtacaagg cactgggcat agtcgcaccg cgcgtccttt caagccgcac tttctaaaaa 15360

aaaaaaaaaa atgtccgttc ttatctcgcc cagtaataac accggttggg gtctgcgcgc 15420

tcccagcaag atgtacggag gcgcacgcaa acgttctacc caacatcccg tgcgtgttcg 15480

cgggcatttt cgcgctccat ggggtgccct caagggccgc actcgcgttc gaaccaccgt 15540

cgatgatgta atcgatcagg tggttgccga cgcccgtaat tatactccta ctgcgcctac 15600

atctactgtg gacgcagtta ttgacagtgt agtggctgac gctcgcaact atgctcgacg 15660

taagagccgg cgaaggcgca ttgccagacg tcaccgagct accactgcca tgcgagcagc 15720

aagagctctg ctacgaagag ctagacgcgt ggggcgaaga gccatgctta gggcggccag 15780

acgtgcagct tcgggcgcca gcgccggcag gtcccgcagg caagcagccg ctgtcgcagc 15840

ggcgactatt gccgacatgg cccaatcgcg aagaggcaat gtatactggg tgcgtgacgc 15900

tgccaccggt caacgtgtac ccgtgcgcac ccgtccccct cgcacttaga agatactgag 15960

cagtctccga tgttgtgtcc cagcggcgag gatgtccaag cgcaaataca aggaagaaat 16020

gctgcaggtt atcgcacctg aagtctacgg ccaaccgttg aaggatgaaa aaaaaccccg 16080

caaaatcaag cgggtaaaaa aggacaaaaa agaagaggaa gatggcgatg atgggctggc 16140

ggagtttgtg cgcgagtttg ccccacggcg acgcgtgcaa tggcgtgggc gcaaagttcg 16200

acatgtgttg agacctggaa cttcggtggt ctttacaccc ggcgagcgtt caagcgctac 16260

ttttaagcgt tcctatgatg aggtgtacgg ggatgatgat attcttgagc aggcagctga 16320

ccgattaggc gagtttgctt atggcaagcg tagtagaata aatcccaagg atgaaacagt 16380

gtccataccc ttggatcatg gaaatcccac ccctagtctt aaaccggtca ctttgcagca 16440

agtgttaccc gtaactccgc gaacaggtgt taaacgcgaa ggtgaagatt tgtatcccac 16500

tatgcaactg atggtgccca aacgccagaa gttggaggac gttttggaga aagtaaaagt 16560

ggatccagat attcaacctg aggttaaagt gagacccatt aagcaggtag cgcctggtct 16620

gggagtacaa actgtagaca ttaaaattcc cactgaaagt atggaagtgc aaactgaacc 16680

cgcaaagcct actgccacct ccactgaagt gcaaacggac ccatggatgc ccatgcctat 16740

tacaactgac gccgtcggtc ccactcgaag atcccgacga aagtacggtc cagcaagtct 16800

gttgatgccc aactatgtcg tacacccatc tattattcct actcctggtt accgaggcac 16860

tcgctactat cgcagccgaa acagtacttc ccgccgtcgc cgcaagacac ctgcaaatcg 16920

cagtcgtcgc cgtagacgca caagcaaacc gattcccggc gccctggtgc ggcaagtgta 16980

ccgcaatggt agtgcggaac ctttgacact gccgcgtgcg cgttaccatc ctagtatcat 17040

cacttaatca atgttgccgc tgcctccttg cagatatggc cctcacttgt cgccttcgcg 17100

ttcccatcac tggttaccga ggaagaaact cgcgccgtag aagagggatg ttggggcgcg 17160

gaatgcgacg ctacaggcga cggcgtgcta tccgcaagca attgcggggt ggttttttgc 17220

cagccttaat tccaattatc gctgctgcga ttggcgcaat accaggcata gcttccgtgg 17280

cggttcaggc ctcgcaacga cattgacatt ggaaaaaaaa aaaacgtata aataaaaaat 17340

acaatggact ctgacactcc tggtactgtg actatgtttt cttagagatg gaagacatca 17400

atttttcatc cttggctccg cgacacggca cgaagccgta catgggcacc tggagcgaca 17460

tcggcacgag ccaactgaac gggggcgcct tcaattggag cagtatctgg agcgggctta 17520

aaaattttgg ctcaaccata aaaacatacg ggaacaaagc ttggaacagc agtacaggac 17580

aggcgcttag aaataaactt aaagaccaga acttccaaca aaaagtagtc gatgggatag 17640

cttccggtat caatggagtg gtagatttgg ctaaccaggc tgtgcagaaa aagataaaca 17700

gtcgtttgga cccgccgcca gcaaccccag gtgaaatgca agtggaggaa gaaattcctc 17760

cgccagaaaa acgaggcgac aagcgtccgc gtcccgattt ggaagagacg ctggtgacgc 17820

gcgtagatga accgccttct tatgaggaag caacgaagct tggaatgccc accactagac 17880

cgatagcccc tatggccacc ggggtgatga aaccttctca gttgcatcga cccgtcacct 17940

tggatttgcc ccctcctcct gctgctactg ctgtacccgc ttctaagcct gtcgctgccc 18000

cgaaaccagt cgccgtagcc aggtcacgtc ccgggggcgc tcctcgtcca aatgcacact 18060

ggcaaaatac tctgaacagc atcgtgggtc taggcgtgca aagtgtaaaa cgccgtcgct 18120

gcttttaatt aaatatggag tagcgcttaa cttgcctatc tgtgtatatg tgtcattaca 18180

cgccgtcaca gcatcagagg aaaaaaggaa gaggtcgtgc gtcgacgctg agttactttc 18240

aagatggcca ccccatcgat gctgccccaa tgggcataca tgcacatcgc cggacaggat 18300

gcttcggagt acctgagtcc gggtctggtg cagttcgccc gcgccacaga cacctacttc 18360

aatctgggaa ataagtttag aaatcctacc gtagcgccga cccacgatgt gaccaccgat 18420

cgtagccagc ggctcatgtt gcgcttcgtg cccgttgacc gggaggacaa tacatactct 18480

tacaaagtgc ggtacaccct ggccgtgggc gacaacagag tgctggatat ggccagcacg 18540

ttctttgaca ttaggggcgt gttggacaga ggtcccagtt ttaaacccta ttctggtacg 18600

gcttacaact ccctggctcc taaaggcgct ccaaatgcat ctcagtggtt ggataaggga 18660

gttacaagca ctggcctagt ggacgacggc aatactgatg atggggaaga agccaaaaaa 18720

gcaacataca cttttggtaa tgctccagta aaagccgagg ctgaaatcac aaaagacgga 18780

ttgccggtgg gcttggaagt ttcaactgaa ggtcctaaac caatctatgc tgataagctt 18840

tatcagccag aacctcaagt gggagacgaa acttggactg acctagacgg aaaaaccgaa 18900

gagtatggag ggagggttct taaacctgaa actaaaatga aaccctgcta cggatctttt 18960

gctaaaccta ctaatattaa aggaggtcag gcaaaggtaa aaccaaaaga agacgatggc 19020

actaacaaca tcgagtatga cattgacatg aacttctttg acttaagatc acaaagatca 19080

gaactcaaac ctaaaattgt aatgtatgca gaaaatgtgg acctggaatg tccagatact 19140

catgttgtgt acaaacctgg agtttcagat gctagttctg agaccaatct tggacaacag 19200

tctatgccca acagacccaa ctacattggc ttcagagata acttcatcgg acttatgtac 19260

tataacagta ctggcaacat gggggtactg gctggccaag cgtctcagtt gaatgcagtg 19320

gttgacttgc aggacagaaa cacagaactg tcttaccaac tcttgcttga ctctctgggc 19380

gacagaacca gatactttag catgtggaat caggctgtgg acagttatga tcctgatgta 19440

cgtgttattg aaaatcatgg tgtggaagat gaacttccca actattgttt tccgttggat 19500

ggtgtcggtc cgcgaacaga tagttacaag gagattaagc caaatggaga ccaatctact 19560

tggacaaatg tagacccaac tggcagcagt gaacttgcta agggaaatcc atttgccatg 19620

gaaattaacc ttcaagccaa tctatggcga agtttccttt attccaatgt ggctctatat 19680

ctcccagact cgtacaaata caccccgtcc aatgtcactc ttccagaaaa caaaaacacc 19740

tacgactaca tgaacgggcg ggtggtgccg ccatctctag tagacaccta tgtgaacatt 19800

ggtgccaggt ggtctctgga tgccatggac aatgtcaacc cattcaacca ccaccgtaac 19860

gctggcttgc gttaccgatc catgcttctg ggtaacggac gttatgtgcc tttccacata 19920

caagtgcctc aaaaattctt cgctgttaaa aacctgctgc ttctcccagg ctcctacact 19980

tatgagtgga actttaggaa ggatgtaaac atggttctac agagttccct cggtaacgac 20040

ctacgggtag atggcgccag catcagtttt acgagcatca acctctatgc tacttttttc 20100

cccatggctc acaacaccgc ttccaccctt gaagccatgc tgcggaatga caccaatgat 20160

cagtcattca acgactacct atctgcagct aacatgctct accccattcc tgccaatgca 20220

accaatattc ccatttccat tccttctcgc aactgggcgg ctttcagagg ctggtcattt 20280

accagactga aaaccaaaga aactccctct ttggggtctg gatttgaccc ctacttcgtc 20340

tattctggtt ctattcccta cctggatggt accttctacc tgaaccacac ttttaagaag 20400

gtttccatca tgtttgactc ttcagtgagc tggcctggaa atgacaggtt actatctcct 20460

aacgaatttg aaataaagcg cactgtggat ggcgaaggct acaacgtagc ccaatgcaac 20520

atgaccaaag actggttctt ggtacagatg ctcgccaact acaacatcgg ctatcagggc 20580

ttctacattc cagaaggata caaagatcgc atgtattcat ttttcagaaa cttccagccc 20640

atgagcaggc aggtggttga tgaggtcaat tacaaagact tcaaggccgt cgccataccc 20700

taccaacaca acaactctgg ctttgtgggt tacatggctc cgaccatgcg tcaaggtcaa 20760

ccctatcccg ctaactatcc ctatccactc attggaacaa ctgccgtaaa tagtgttacg 20820

cagaaaaagt tcttgtgtga cagaaccatg tggcgcatac cgttctcaag caacttcatg 20880

tctatgggag cccttacaga cttgggacag aacatgctct atgccaactc agctcatgct 20940

ctggacatga cctttgaggt ggatcccatg gatgagccca ccctgcttta tcttctcttc 21000

gaagttttcg acgtggtcag agtgcatcag ccacaccgcg gcatcatcga ggcagtctac 21060

ctgcgtacac cgttctcggc cggtaacgct accacgtaag aagcttcttg cttcttgcaa 21120

acagcagctg caaccatggc ctgcggatcc caaaacggct ccagcgagca agagctcaga 21180

gccattgtcc aagacctggg ttgcggacca tattttttgg gaacctttga taagcgcttc 21240

ccggggttca tggcccccga taagctcgcc tgtgccattg taaatacggc cggacgtgag 21300

acggggggag agcactggtt ggctttcggt tggaacccac gttctaacac ctgctacctt 21360

tttgatcctt ttggattctc ggatgatcgt ctcaaacaga tttaccagtt tgaatatgag 21420

ggtctcctgc gccgcagcgc tcttgctacc aaggaccggt gtattacgct ggaaaaatct 21480

acccagaccg tgcagggccc ccgttctgcc gcctgcggac ttttctgctg catgttcctt 21540

catgcctttg tgcactggcc tgaccgtccc atggacggaa accccaccat gaaattgcta 21600

actggagtgc caaacaacat gcttcattct cctaaagtcc agcccaccct gtgtgacaat 21660

caaaaagcac tctaccattt tctcaatacc cattcgcctt attttcgctc tcatcgtaca 21720

cacatcgaaa gggccactgc gttcgaccgt atggatgtgc aataatgatt catgtaaaca 21780

acgtgttcaa taaacagcac tttatttttt acatgtatcg aggctctgga ttacttattt 21840

atttacaagt cgaatgggtt ctgacgagaa tcagaatgac ccgcaggcag tgatacgttg 21900

cggaactgat acttgggttg ccacttgaat tcgggaatca ccaacttggg aaccggtata 21960

tcgggcagga tgtcactcca cagctttctg gtcagctgca aagctcccag caggtcagga 22020

gccgaaatct tgaaatcaca attaggacca gtgctctgag cgcgagagtt gcggtacacc 22080

ggattgcagc actgaaacac catcagcgac ggatgtctta cgcttgccag cacggtggga 22140

tctgcaatca tgcccacatc cagatcttca gcattggcaa tgctgaacgg ggtcatcttg 22200

caggtctgcc tacccatggc gggcacccaa ttaggcttgt ggttacaatc gcagtgcagg 22260

gggatcagta tcatcttggc ctgatcctgt ctgattcctg gatacacggc tctcatgaaa 22320

gcatcatatt gcttgaaagc ctgctgggct ttactaccct cggtataaaa catcccgcag 22380

gacctgctcg aaaactggtt agctgcgcag ccggcatcat tcacacagca gcgggcgtca 22440

ttgttggcta tttgcaccac acttctgccc cagcggtttt gggtgatttt ggttcgctcg 22500

ggattctcct tcaaggctcg ttgtccgttc tcgctggcca catccatctc gataatctgc 22560

tccttctgaa tcataatatt gccatgcaag cacttcagct tgccctcata atcattgcag 22620

ccatgaggcc acaacgcaca gcctgtacat tcccaattat ggtgggcgat ctgagaaaaa 22680

gaatgtatca ttccctgcag aaatcttccc atcatcgtgc tcagtgtctt gtgactagtg 22740

aaagttaact ggatgcctcg gtgctcctcg ttcacgtact ggtgacagat gcgcttgtat 22800

tgttcgtgct gctcaggcat tagtttaaaa gaggttctaa gttcgttatc cagcctgtac 22860

ttctccatca gcagacacat cacttccatg cctttctccc aagcagacac caggggcaag 22920

ctaatcggat tcttaacagt gcaggcagca gctcctttag ccagagggtc atctttggcg 22980

atcttctcaa tgcttctttt gccatccttc tcaacgatgc gcacgggcgg gtagctgaaa 23040

cccactgcta caagttgcgc ctcttctctt tcttcttcgc tgtcttgact gatgtcttgc 23100

atggggacat gtttggtctt ccttggcttc tttttcgggg gtatcggagg aggaggactg 23160

tcgctccgtt ccggagacag ggaggattgt gacgtttcgc tcaccattac caactgactg 23220

tcggtagaag aacctgaccc cacacggcga caggtgtttc tcttcggggg cagaggtgga 23280

ggcgattgcg aagggctgcg gtccgacctg gaaggcggat gactggcaga accccttccg 23340

cgttcggggg tgtgctccct gtggcggtcg cttaactgat ttccttcgcg gctggccatt 23400

gtgttctcct aggcagagaa acaacagaca tggaaactca gccattgctg tcaacatcgc 23460

cacgagtgcc atcacatctc gtcctcagcg acgaggaaaa ggagcagagc ttaagcattc 23520

caccgcccag tcctgccacc acctctaccc tagaagataa ggaggtcgac gcatctcatg 23580

acatgcagaa taaaaaagcg aaagagtctg agccagacat cgaacaagac ccgggctatg 23640

tgacaccggt ggaacacgag gaagagttga aacgctttct agagagagag gatgaaaact 23700

gcccaaaaca gcaagcggat aactatcacc aagatgctgg aaatagggat cagaacaccg 23760

actacctcat agggcttgac ggggaagacg cgctccttaa acatctagca agacagtcac 23820

tcatagtcaa ggatgcatta ttggacagaa ctgaagtgcc catcagtgtc gaagagctca 23880

gccgcgccta cgagcttaac ctattttcac ctcgtactcc ccccaaacgt cagccaaacg 23940

gcacctgcga gccaaatcct cgcttaaact tttatccagc ttttgctgtg ccagaagtac 24000

tggctaccta tcacatcttt tttaaaaatc aaaaaattcc agtctcctgc cgcgctaatc 24060

gcacccgcgc cgatgcccta ctcaatctgg gacctggttc acgcttacct gatatagctt 24120

ccttggaaga ggttccaaag atcttcgagg gtctgggcaa taatgagact cgggccgcaa 24180

atgctctgca aaagggagaa aatggcatgg atgagcatca cagcgttctg gtggaattgg 24240

aaggcgataa tgccagactc gcagtactca agcgaagcgt cgaggtcaca cactttgcat 24300

accccgctgt caacctgccc cctaaagtca tgacggccgt catggaccag ttactcatta 24360

agcgcgcaag tcccctttca gaagacatgc atgacccaga tgcctgtgat gagggtaaac 24420

cagtggtcag tgatgagcag ctaacccgat ggctgggcac cgactctccc cgggatttgg 24480

aagagcgtcg caagcttatg atggccgtgg tgctggttac cgtagaacta gagtgtcttc 24540

ggcgtttctt taccgattca gaaaccttgc gcaaactcga agagaatctg cactacactt 24600

ttagacacgg ctttgtgcgg caggcatgca agatatctaa cgtggaactc accaacctgg 24660

tttcctacat gggtattctg catgagaatc gcctaggaca aagcgtgctg cacagcaccc 24720

ttaaggggga agcccgccgt gattacatcc gcgattgtgt ttatctctac ctgtgccaca 24780

cgtggcaaac cggcatgggt gtatggcagc aatgtttaga agaacagaac ctgaaagagc 24840

taaacaagct cttacagaaa tctcttaagg ttctgtggac agggttcgac gagcgcaccg 24900

tcgcttccga cctggcagac ctcatcttcc cagagcgtct cagggttact ttgcgaaacg 24960

gactgcctga ctttatgagc cagagcatgc ttaacaattt tcgctctttc atcctggaac 25020

gctccggtat cctgcccgcc acctgctgcg cactgccctc cgactttgtg cctctcacct 25080

accgcgaatg ccccccgccg ctatggagtc actgctacct gttccgtctg gccaactacc 25140

tctcctacca ctcggatgtg atcgaggatg tgagcggaga cggcttgctg gagtgtcact 25200

gccgctgcaa tctgtgcacg ccccaccggt ccctagcttg caacccccag ttgatgagcg 25260

aaacccagat aataggcacc tttgaattgc aaggccccag cagccaaggc gatgggtctt 25320

ctcctgggca aagtttaaaa ctgaccccgg gactgtggac ctccgcctac ttgcgcaagt 25380

ttgccccgga agattaccac ccctatgaaa tcaagttcta tgaggaccaa tcacagcctc 25440

cgaaagccga actttcggcc tgcgtcatca cccagggggc aattctggcc caattgcaag 25500

ccatccaaaa atcccgccaa gaatttctac tgaaaaaggg taagggggtc taccttgacc 25560

cccagaccgg cgaggaactc aacacaaggt tccctcagga tgtcccaacg acgagaaagc 25620

aagaagttga aggtgcagcc gccgccccca gaagatatgg aggaagattg ggacagtcag 25680

gcagaggaag cggaggagga ggacagtctg gaggacagtc tggaggaaga cagtttggag 25740

gaggaaaacg aggaggcaga ggaggtggaa gaagtaaccg ccgacaaaca gttatcctcg 25800

gctgcggaga caagcaacag cgctaccatc tccgctccga gtcgaggaac ccggcggcgt 25860

cccagcagta gatgggacga gaccggacgc ttcccgaacc caaccagcgc ttccaagacc 25920

ggtaagaagg atcggcaggg atacaagtcc tggcgggggc ataagaatgc catcatctcc 25980

tgcttgcatg agtgcggggg caacatatcc ttcacgcggc gctacttgct attccaccat 26040

ggggtgaact ttccgcgcaa tgttttgcat tactaccgtc acctccacag cccctactat 26100

agccagcaaa tcccggcagt ctcgacagat aaagacagcg gcggcgacct ccaacagaaa 26160

accagcagcg gcagttagaa aatacacaac aagtgcagca acaggaggat taaagattac 26220

agccaacgag ccagcgcaaa cccgagagtt aagaaatcgg atctttccaa ccctgtatgc 26280

catcttccag cagagtcggg gccaagagca ggaactgaaa ataaaaaacc gatctctgcg 26340

ttcgctcacc agaagttgtt tgtatcacaa gagcgaagat caacttcagc gcactctcga 26400

ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt aggcagcgac 26460

cgcgcttatt caaaaaaggc gggaattaca tcatcctcga catgagtaaa gaaattccca 26520

cgccttacat gtggagttat cagccccaaa tgggattggc ggcaggcgcc tcccaggact 26580

actccacccg catgaattgg ctcagcgccg ggccttctat gatttctcga gttaatgata 26640

tacgcgccta ccgaaaccaa atacttttgg aacagtcagc tcttaccacc acgccccgcc 26700

aacaccttaa tcccagaaat tggcccgccg ccctagtgta ccaggaaagt cccgctccca 26760

ccactgtatt acttcctcga gacgcccagg ccgaagtcca aatgactaat gcaggtgcgc 26820

agttagcggg cggctccacc ctatgtcgtc acaggcctcg gcataatata aaacgcctga 26880

tgatcagagg ccgaggtatc cagctcaacg acgagtcggt gagctctccg cttggtctac 26940

gaccagacgg aatctttcag attgccggct gcgggagatc ttccttcacc cctcgtcagg 27000

ctgttctgac tttggaaagt tcgtcttcgc aaccccgctc gggcggaatc gggaccgttc 27060

aatttgtgga ggagtttact ccctctgtct acttcaaccc cttctccgga tctcctgggc 27120

actacccgga cgagttcata ccgaacttcg acgcgattag cgagtcagtg gacggctacg 27180

attgatgtct ggtgacgcgg ctgagctatc tcggctgcga catctagacc actgccgccg 27240

ctttcgctgc tttgcccggg aactcattga gttcatctac ttcgaactcc ccaaggatca 27300

ccctcaaggt ccggcccacg gagtgcggat tactatcgaa ggcaaaatac actctcgcct 27360

gcaacgaatt ttctcccagc ggcccgtgct gatcgagcga gaccagggaa acaccacggt 27420

ttccatctac tgcatttgta atcaccccgg attgcatgaa agcctttgct gtcttatgtg 27480

tactgagttt aataaaaact gaattaagac tctcctacgg actgccgctt cttcaacccg 27540

gattttacaa ccagaagaac gaaacttttc ctgtcgtcca ggactctgtt aacttcacct 27600

ttcctactca caaactagaa gctcaacgac tacaccgctt ttccagaagc attttcccta 27660

ctaatactac tttcaaaacc ggaggtgagc tccaaggtct tcctacagaa aacccttggg 27720

tggaagcggg ccttgtagtg ctaggaattc ttgcgggtgg gcttgtgatt attctttgct 27780

acctatacac accttgcttc actttcctag tggtgttgtg gtattggttt aaaaaatggg 27840

gcccatacta gtcttgcttg ttttactttc gcttttggaa ccgggttctg ccaattacga 27900

tccatgtcta gacttcgacc cagaaaactg cacacttact tttgcacccg acacaagccg 27960

catctgtgga gttcttatta agtgcggatg ggaatgcagg tccgttgaaa ttacacacaa 28020

taacaaaacc tggaacaata ccttatccac cacatgggag ccaggagttc ccgagtggta 28080

cactgtctct gtccgaggtc ctgacggttc catccgcatt agtaacaaca ctttcatttt 28140

ttctgaaatg tgcgatctgg ccatgttcat gagcaaacag tattctctat ggcctcctag 28200

caaggacaac atcgtaacgt tctccattgc ttattgcttg tgcgcttgcc ttcttactgc 28260

tttactgtgc gtatgcatac acctgcttgt aaccactcgc atcaaaaacg ccaataacaa 28320

agaaaaaatg ccttaacctc tttctgttta cagacatggc ttctcttaca tctctcatat 28380

ttgtcagcat tgtcactgcc gctcacggac aaacagtcgt ctctatccct ctaggacata 28440

attacactct cataggaccc ccaatcactt cagaggtcat ctggaccaaa ctgggaagcg 28500

ttgattactt tgatataatc tgcaacaaaa caaaaccaat aatagtaact tgcaacatac 28560

aaaatcttac attgattaat gttagcaaag tttacagcgg ttactattat ggttatgaca 28620

gatacagtag tcaatataga aattacttgg ttcgtgttac ccagttaaaa accacgaaaa 28680

tgccaaatat ggcaaagatt cgatccgatg acaattctct agaaactttt acatctccca 28740

ccacacccga cgaaaaaaac atcccagatt caatgattgc aattgttgca gcggtggcag 28800

tggtgatggc actaataata atatgcatgc ttttatatgc ttgtcgctac aaaaagtttc 28860

atcctaaaaa acaagatctc ctactaaggc ttaacattta atttcttttt atacagccat 28920

ggtttccact accacattcc ttatgcttac tagtcttgca actctgactt ctgctcgctc 28980

acacctcact gtaactatag gctcaaactg cacactaaaa ggacctcaag gtggtcatgt 29040

cttttggtgg agaatatatg acaatggatg gtttacaaaa ccatgtgacc aacctggtag 29100

atttttctgc aacggcagag acctaaccat tatcaacgtg acagcaaatg acaaaggctt 29160

ctattatgga accgactata aaagtagttt agattataac attattgtac tgccatctac 29220

cactccagca ccccgcacaa ctactttctc tagcagcagt gtcgctaaca atacaatttc 29280

caatccaacc tttgccgcgc ttttaaaacg cactgtgaat aattctacaa cttcacatac 29340

aacaatttcc acttcaacaa tcagcattat cgctgcagtg acaattggaa tatctattct 29400

tgtttttacc ataacctact acgcctgctg ctatagaaaa gacaaacata aaggtgatcc 29460

attacttaga tttgatattt aatttgttct tttttttttt atttacagta tggtgaacac 29520

caatcatggt acctagaaat ttcttcttca ccatactcat ttgtgcattt aatgtttgcg 29580

ctactttcac agcagtagcc acagcaaccc cagactgtat aggagcattt gcttcctatg 29640

cactttttgc ttttgttact tgcatctgcg tatgtagcat agtctgcctg gttattaatt 29700

ttttccaact tctagactgg atccttgtgc gaattgccta cctgcgccac catcccgaat 29760

accgcaacca aaatatcgcg gcacttctta gactcatcta aaaccatgca ggctatacta 29820

ccaatatttt tgcttctatt gcttccctac gctgtctcaa ccccagctgc ctatagtact 29880

ccaccagaac accttagaaa atgcaaattc caacaaccgt ggtcatttct tgcttgctat 29940

cgagaaaaat cagaaattcc cccaaattta ataatgattg ctggaataat taatataatc 30000

tgttgcacca taatttcatt tttgatatac cccctatttg attttggctg gaatgctccc 30060

aatgcacatg atcatccaca agacccagag gaacacattc ccctacaaaa catgcaacat 30120

ccaatagcgc taatagatta cgaaagtgaa ccacaacccc cactactccc tgctattagt 30180

tacttcaacc taaccggcgg agatgactga aacactcacc acctccaatt ccgccgagga 30240

tctgctcgat atggacggcc gcgtctcaga acagcgactt gcccaactac gcatccgcca 30300

gcagcaggaa cgcgcggcca aagagctcag agatgtcatc caaattcacc aatgcaaaaa 30360

aggcatattc tgtttggtaa aacaagccaa gatatcctac gagatcaccg ctactgacca 30420

tcgcctctct tacgaacttg gcccccaacg acaaaaattt acctgcatgg tgggaatcaa 30480

ccccatagtt atcacccagc aaagtggaga tactaagggt tgcattcact gctcctgcga 30540

ttccatcgag tgcacctaca ccctgctgaa gaccctatgc ggcctaagag acctgctacc 30600

aatgaattaa aaaatgatta ataaaaaatc acttacttga aatcagcaat aaggtctctg 30660

ttgaaatttt ctcccagcag cacctcactt ccctcttccc aactctggta ttctaaaccc 30720

cgttcagcgg catactttct ccatacttta aaggggatgt caaattttag ctcctctcct 30780

gtacccacaa tcttcatgtc tttcttccca gatgaccaag agagtccggc tcagtgactc 30840

cttcaaccct gtctacccct atgaagatga aagcacctcc caacacccct ttataaaccc 30900

agggtttatt tccccaaatg gcttcacaca aagcccagac ggagttctta ctttaaaatg 30960

tttaacccca ctaacaacca caggcggatc tctacagcta aaagtgggag ggggacttac 31020

agtggatgac actgatggta ccttacaaga aaacatacgt gctacagcac ccattactaa 31080

aaataatcac tctgtagaac tatccattgg aaatggatta gaaactcaaa acaataaact 31140

atgtgccaaa ttgggaaatg ggttaaaatt taacaacggt gacatttgta taaaggatag 31200

tattaacacc ttatggactg gaataaaccc tccacctaac tgtcaaattg tggaaaacac 31260

taatacaaat gatggcaaac ttactttagt attagtaaaa aacggagggc ttgttaatgg 31320

ctacgtgtct ctagttggtg tatcagacac tgtgaaccaa atgttcacac aaaagacagc 31380

aaacatccaa ttaagattat attttgactc ttctggaaat ctattaactg atgaatcaga 31440

cttaaaaatt ccacttaaaa ataaatcttc tacagcgacc agtgaaactg tagccagcag 31500

caaagccttt atgccaagta ctacagctta tcccttcaac accactacta gggatagtga 31560

aaactacatt catggaatat gttactacat gactagttat gatagaagtc tatttccctt 31620

gaacatttct ataatgctaa acagccgtat gatttcttcc aatgttgcct atgccataca 31680

atttgaatgg aatctaaatg caagtgaatc tccagaaagc aacatagcta cgctgaccac 31740

atcccccttt ttcttttctt acattacaga agacgacaac taaaataaag tttaagtgtt 31800

tttatttaaa atcacaaaat tcgagtagtt attttgcctc caccttccca tttgacagaa 31860

tacaccaatc tctccccacg cacagcttta aacatttgga taccattaga gatagacatt 31920

gttttagatt ccacattcca aacagtttca gagcgagcca atctggggtc agtgatagat 31980

aaaaatccat cgcgatagtc ttttaaagcg ctttcacagt ccaactgctg cggatgcgaa 32040

tccggagtct ggatcacggt catctggaag aagaacgatg ggaatcataa tccgaaaacg 32100

gtatcggacg attgtgtctc atcaaaccca caagcagccg ctgtctgcgt cgctccgtgc 32160

aactgctgtt tatgggatca gggtccacag tgtcctgaag catgatttta atagccctta 32220

acatcaactt tctggtgcga tgcgcgcagc aacgcattct gatttcactc aaatctttgc 32280

agtaggtaca acacattatt acaatattgt ttaataaacc ataattaaaa gcgctccagc 32340

caaaactcat atctgatata atcgcccctg catgaccatc ataccaaagt ttaatataaa 32400

ttaaatgacg ttccctcaaa aacacactac ccacatacat gatctctttt ggcatgtgca 32460

tattaacaat ctgtctgtac catggacaac gttggttaat catgcaaccc aatataacct 32520

tccggaacca cactgccaac accgctcccc cagccatgca ttgaagtgaa ccctgctgat 32580

tacaatgaca atgaagaacc caattctctc gaccgtgaat cacttgagaa tgaaaaatat 32640

ctatagtggc acaacataga cataaatgca tgcatcttct cataattttt aactcctcag 32700

gatttagaaa catatcccag ggaataggaa gctcttgcag aacagtaaag ctggcagaac 32760

aaggaagacc acgaacacaa cttacactat gcatagtcat agtatcacaa tctggcaaca 32820

gcgggtggtc ttcagtcata gaagctcggg tttcattttc ctcacaacgt ggtaactggg 32880

ctctggtgta agggtgatgt ctggcgcatg atgtcgagcg tgcgcgcaac cttgtcataa 32940

tggagttgct tcctgacatt ctcgtatttt gtatagcaaa acgcggccct ggcagaacac 33000

actcttcttc gccttctatc ctgccgctta gcgtgttccg tgtgatagtt caagtacagc 33060

cacactctta agttggtcaa aagaatgctg gcttcagttg taatcaaaac tccatcgcat 33120

ctaattgttc tgaggaaatc atccacggta gcatatgcaa atcccaacca agcaatgcaa 33180

ctggattgcg tttcaagcag gagaggagag ggaagagacg gaagaaccat gttaattttt 33240

attccaaacg atctcgcagt acttcaaatt gtagatcgcg cagatggcat ctctcgcccc 33300

cactgtgttg gtgaaaaagc acagctaaat caaaagaaat gcgattttca aggtgctcaa 33360

cggtggcttc caacaaagcc tccacgcgca catccaagaa caaaagaata ccaaaagaag 33420

gagcattttc taactcctca atcatcatat tacattcctg caccattccc agataatttt 33480

cagctttcca gccttgaatt attcgtgtca gttcttgtgg taaatccaat ccacacatta 33540

caaacaggtc ccggagggcg ccctccacca ccattcttaa acacaccctc ataatgacaa 33600

aatatcttgc tcctgtgtca cctgtagcga attgagaatg gcaacatcaa ttgacatgcc 33660

cttggctcta agttcttctt taagttctag ttgtaaaaac tctctcatat tatcaccaaa 33720

ctgcttagcc agaagccccc cgggaacaag agcaggggac gctacagtgc agtacaagcg 33780

cagacctccc caattggctc cagcaaaaac aagattggaa taagcatatt gggaaccgcc 33840

agtaatatca tcgaagttgc tggaaatata atcaggcaga gtttcttgta aaaattgaat 33900

aaaagaaaaa tttgccaaaa aaacattcaa aacctctggg atgcaaatgc aataggttac 33960

cgcgctgcgc tccaacattg ttagttttga attagtctgc aaaaataaaa aaaaaaacaa 34020

gcgtcatatc atagtagcct gacgaacagg tggataaatc agtctttcca tcacaagaca 34080

agccacaggg tctccagctc gaccctcgta aaacctgtca tggtgattaa acaacagcac 34140

cgaaagttcc tcgcggtgac cagcatgaat aattcttgat gaagcataca atccagacat 34200

gttagcatca gttaacgaga aaaaacagcc aacatagcct ttgggtataa ttatgcttaa 34260

tcgtaagtat agcaaagcca cccctcgcgg atacaaagta aaaggcacag gagaataaaa 34320

aatataatta tttctctgct gctgttcagg caacgtcgcc cccggtccct ctaaatacac 34380

atacaaagcc tcatcagcca tggcttacca gacaaagtac agcgggcacg cacaagctct 34440

aaagtcactc tccaacctct ccacaatata tatacacaag ccctaaactg acgtaatggg 34500

agtaaagtgt aaaaaatccc gccaaaccca acacacaccc cgaaactgcg tcaccaggga 34560

aaagtacagt ttcacttccg caatcccaac aagcgtcact tcctctttct cacggtacgt 34620

cacatcccat taacttgcaa cgtcattttc ccacggccgc gccgccccgt ttagccgtta 34680

accccacagc caatcaccac acaccccaca atttttaaaa tcacctcatt tacatattgg 34740

caccattcca tctataaggt atattattga tgatg 34775



2
49
DNA
Artificial Sequence

synthetic polyA signal


2

aataaaagat ctttattttc attagatctg tgtgttggtt ttttgtgtg 49



3
1521
DNA
Artificial Sequence

optimized human HIV-1 gag open reading frame


3

atgggtgcta gggcttctgt gctgtctggt ggtgagctgg acaagtggga gaagatcagg 60

ctgaggcctg gtggcaagaa gaagtacaag ctaaagcaca ttgtgtgggc ctccagggag 120

ctggagaggt ttgctgtgaa ccctggcctg ctggagacct ctgaggggtg caggcagatc 180

ctgggccagc tccagccctc cctgcaaaca ggctctgagg agctgaggtc cctgtacaac 240

acagtggcta ccctgtactg tgtgcaccag aagattgatg tgaaggacac caaggaggcc 300

ctggagaaga ttgaggagga gcagaacaag tccaagaaga aggcccagca ggctgctgct 360

ggcacaggca actccagcca ggtgtcccag aactacccca ttgtgcagaa cctccagggc 420

cagatggtgc accaggccat ctccccccgg accctgaatg cctgggtgaa ggtggtggag 480

gagaaggcct tctcccctga ggtgatcccc atgttctctg ccctgtctga gggtgccacc 540

ccccaggacc tgaacaccat gctgaacaca gtggggggcc atcaggctgc catgcagatg 600

ctgaaggaga ccatcaatga ggaggctgct gagtgggaca ggctgcatcc tgtgcacgct 660

ggccccattg cccccggcca gatgagggag cccaggggct ctgacattgc tggcaccacc 720

tccaccctcc aggagcagat tggctggatg accaacaacc cccccatccc tgtgggggaa 780

atctacaaga ggtggatcat cctgggcctg aacaagattg tgaggatgta ctcccccacc 840

tccatcctgg acatcaggca gggccccaag gagcccttca gggactatgt ggacaggttc 900

tacaagaccc tgagggctga gcaggcctcc caggaggtga agaactggat gacagagacc 960

ctgctggtgc agaatgccaa ccctgactgc aagaccatcc tgaaggccct gggccctgct 1020

gccaccctgg aggagatgat gacagcctgc cagggggtgg ggggccctgg tcacaaggcc 1080

agggtgctgg ctgaggccat gtcccaggtg accaactccg ccaccatcat gatgcagagg 1140

ggcaacttca ggaaccagag gaagacagtg aagtgcttca actgtggcaa ggtgggccac 1200

attgccaaga actgtagggc ccccaggaag aagggctgct ggaagtgtgg caaggagggc 1260

caccagatga aggactgcaa tgagaggcag gccaacttcc tgggcaaaat ctggccctcc 1320

cacaagggca ggcctggcaa cttcctccag tccaggcctg agcccacagc ccctcccgag 1380

gagtccttca ggtttgggga ggagaagacc acccccagcc agaagcagga gcccattgac 1440

aaggagctgt accccctggc ctccctgagg tccctgtttg gcaacgaccc ctcctcccag 1500

taaaataaag cccgggcaga t 1521



4
2550
DNA
Artificial Sequence

gag expression cassette


4

ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca 60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca 120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 660

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 720

caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 780

gccaagagtg agatctacca tgggtgctag ggcttctgtg ctgtctggtg gtgagctgga 840

caagtgggag aagatcaggc tgaggcctgg tggcaagaag aagtacaagc taaagcacat 900

tgtgtgggcc tccagggagc tggagaggtt tgctgtgaac cctggcctgc tggagacctc 960

tgaggggtgc aggcagatcc tgggccagct ccagccctcc ctgcaaacag gctctgagga 1020

gctgaggtcc ctgtacaaca cagtggctac cctgtactgt gtgcaccaga agattgatgt 1080

gaaggacacc aaggaggccc tggagaagat tgaggaggag cagaacaagt ccaagaagaa 1140

ggcccagcag gctgctgctg gcacaggcaa ctccagccag gtgtcccaga actaccccat 1200

tgtgcagaac ctccagggcc agatggtgca ccaggccatc tccccccgga ccctgaatgc 1260

ctgggtgaag gtggtggagg agaaggcctt ctcccctgag gtgatcccca tgttctctgc 1320

cctgtctgag ggtgccaccc cccaggacct gaacaccatg ctgaacacag tggggggcca 1380

tcaggctgcc atgcagatgc tgaaggagac catcaatgag gaggctgctg agtgggacag 1440

gctgcatcct gtgcacgctg gccccattgc ccccggccag atgagggagc ccaggggctc 1500

tgacattgct ggcaccacct ccaccctcca ggagcagatt ggctggatga ccaacaaccc 1560

ccccatccct gtgggggaaa tctacaagag gtggatcatc ctgggcctga acaagattgt 1620

gaggatgtac tcccccacct ccatcctgga catcaggcag ggccccaagg agcccttcag 1680

ggactatgtg gacaggttct acaagaccct gagggctgag caggcctccc aggaggtgaa 1740

gaactggatg acagagaccc tgctggtgca gaatgccaac cctgactgca agaccatcct 1800

gaaggccctg ggccctgctg ccaccctgga ggagatgatg acagcctgcc agggggtggg 1860

gggccctggt cacaaggcca gggtgctggc tgaggccatg tcccaggtga ccaactccgc 1920

caccatcatg atgcagaggg gcaacttcag gaaccagagg aagacagtga agtgcttcaa 1980

ctgtggcaag gtgggccaca ttgccaagaa ctgtagggcc cccaggaaga agggctgctg 2040

gaagtgtggc aaggagggcc accagatgaa ggactgcaat gagaggcagg ccaacttcct 2100

gggcaaaatc tggccctccc acaagggcag gcctggcaac ttcctccagt ccaggcctga 2160

gcccacagcc cctcccgagg agtccttcag gtttggggag gagaagacca cccccagcca 2220

gaagcaggag cccattgaca aggagctgta ccccctggcc tccctgaggt ccctgtttgg 2280

caacgacccc tcctcccagt aaaataaagc ccgggcagat ctgatctgct gtgccttcta 2340

gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 2400

ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 2460

attctattct ggggggtggg gtggggcagc acagcaaggg ggaggattgg gaagacaata 2520

gcaggcatgc tggggatgcg gtgggctcta 2550



5
2662
DNA
Artificial Sequence

SEAP expression cassette


5

ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca 60

ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca 120

ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 180

ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 240

acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 300

ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 360

aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 420

tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 480

gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 540

gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 600

ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 660

ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 720

caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 780

gccaagagtg agatctaagt aagcttcctg catgctgctg ctgctgctgc tgctgggcct 840

gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa 900

ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc 960

caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag 1020

gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg 1080

cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg 1140

agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag 1200

tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat 1260

gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca 1320

cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga 1380

cgtgcctgcc tccgcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa 1440

catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc 1500

agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct 1560

ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct 1620

catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga 1680

catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga 1740

ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg 1800

tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat 1860

gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct 1920

cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc 1980

catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata 2040

cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga 2100

gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc 2160

aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca 2220

ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc 2280

ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt aacccgtggt 2340

ccccgcgttg cttcctctgc tggccgggac atcaggtggc ccccgctgaa ttggaatcga 2400

tcagaattca gtcgacgata tctgatcacg atctgatctg ctgtgccttc tagttgccag 2460

ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 2520

gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 2580

ctggggggtg gggtggggca gcacagcaag ggggaggatt gggaagacaa tagcaggcat 2640

gctggggatg cggtgggctc ta 2662



6
37474
DNA
Artificial Sequence

pMRKAd5HIV-1 gag, coding


6

ttcttaatta acatcatcaa taatatacct tattttggat tgaagccaat atgataatga 60

gggggtggag tttgtgacgt ggcgcggggc gtgggaacgg ggcgggtgac gtagtagtgt 120

ggcggaagtg tgatgttgca agtgtggcgg aacacatgta agcgacggat gtggcaaaag 180

tgacgttttt ggtgtgcgcc ggtgtacaca ggaagtgaca attttcgcgc ggttttaggc 240

ggatgttgta gtaaatttgg gcgtaaccga gtaagatttg gccattttcg cgggaaaact 300

gaataagagg aagtgaaatc tgaataattt tgtgttactc atagcgcgta atatttgtct 360

agggccgcgg ggactttgac cgtttacgtg gagactcgcc caggtgtttt tctcaggtgt 420

tttccgcgtt ccgggtcaaa gttggcgttt tattattata ggcggccgcg atccattgca 480

tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc 540

atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca 600

tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc 660

gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat 720

agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt 780

acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 840

cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta 900

cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg 960

atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt 1020

gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac 1080

gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa 1140

ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga 1200

ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc gtgccaagag 1260

tgagatctac catgggtgct agggcttctg tgctgtctgg tggtgagctg gacaagtggg 1320

agaagatcag gctgaggcct ggtggcaaga agaagtacaa gctaaagcac attgtgtggg 1380

cctccaggga gctggagagg tttgctgtga accctggcct gctggagacc tctgaggggt 1440

gcaggcagat cctgggccag ctccagccct ccctgcaaac aggctctgag gagctgaggt 1500

ccctgtacaa cacagtggct accctgtact gtgtgcacca gaagattgat gtgaaggaca 1560

ccaaggaggc cctggagaag attgaggagg agcagaacaa gtccaagaag aaggcccagc 1620

aggctgctgc tggcacaggc aactccagcc aggtgtccca gaactacccc attgtgcaga 1680

acctccaggg ccagatggtg caccaggcca tctccccccg gaccctgaat gcctgggtga 1740

aggtggtgga ggagaaggcc ttctcccctg aggtgatccc catgttctct gccctgtctg 1800

agggtgccac cccccaggac ctgaacacca tgctgaacac agtggggggc catcaggctg 1860

ccatgcagat gctgaaggag accatcaatg aggaggctgc tgagtgggac aggctgcatc 1920

ctgtgcacgc tggccccatt gcccccggcc agatgaggga gcccaggggc tctgacattg 1980

ctggcaccac ctccaccctc caggagcaga ttggctggat gaccaacaac ccccccatcc 2040

ctgtggggga aatctacaag aggtggatca tcctgggcct gaacaagatt gtgaggatgt 2100

actcccccac ctccatcctg gacatcaggc agggccccaa ggagcccttc agggactatg 2160

tggacaggtt ctacaagacc ctgagggctg agcaggcctc ccaggaggtg aagaactgga 2220

tgacagagac cctgctggtg cagaatgcca accctgactg caagaccatc ctgaaggccc 2280

tgggccctgc tgccaccctg gaggagatga tgacagcctg ccagggggtg gggggccctg 2340

gtcacaaggc cagggtgctg gctgaggcca tgtcccaggt gaccaactcc gccaccatca 2400

tgatgcagag gggcaacttc aggaaccaga ggaagacagt gaagtgcttc aactgtggca 2460

aggtgggcca cattgccaag aactgtaggg cccccaggaa gaagggctgc tggaagtgtg 2520

gcaaggaggg ccaccagatg aaggactgca atgagaggca ggccaacttc ctgggcaaaa 2580

tctggccctc ccacaagggc aggcctggca acttcctcca gtccaggcct gagcccacag 2640

cccctcccga ggagtccttc aggtttgggg aggagaagac cacccccagc cagaagcagg 2700

agcccattga caaggagctg taccccctgg cctccctgag gtccctgttt ggcaacgacc 2760

cctcctccca gtaaaataaa gcccgggcag atctgctgtg ccttctagtt gccagccatc 2820

tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 2880

ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 2940

gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 3000

ggatgcggtg ggctctatgg ccgatcggcg cgccgtactg aaatgtgtgg gcgtggctta 3060

agggtgggaa agaatatata aggtgggggt cttatgtagt tttgtatctg ttttgcagca 3120

gccgccgccg ccatgagcac caactcgttt gatggaagca ttgtgagctc atatttgaca 3180

acgcgcatgc ccccatgggc cggggtgcgt cagaatgtga tgggctccag cattgatggt 3240

cgccccgtcc tgcccgcaaa ctctactacc ttgacctacg agaccgtgtc tggaacgccg 3300

ttggagactg cagcctccgc cgccgcttca gccgctgcag ccaccgcccg cgggattgtg 3360

actgactttg ctttcctgag cccgcttgca aacagtgcag cttcccgttc atccgcccgc 3420

gatgacaagt tgacggctct tttggcacaa ttggattctt tgacccggga acttaatgtc 3480

gtttctcagc agctgttgga tctgcgccag caggtttctg ccctgaaggc ttcctcccct 3540

cccaatgcgg tttaaaacat aaataaaaaa ccagactctg tttggatttg gatcaagcaa 3600

gtgtcttgct gtctttattt aggggttttg cgcgcgcggt aggcccggga ccagcggtct 3660

cggtcgttga gggtcctgtg tattttttcc aggacgtggt aaaggtgact ctggatgttc 3720

agatacatgg gcataagccc gtctctgggg tggaggtagc accactgcag agcttcatgc 3780

tgcggggtgg tgttgtagat gatccagtcg tagcaggagc gctgggcgtg gtgcctaaaa 3840

atgtctttca gtagcaagct gattgccagg ggcaggccct tggtgtaagt gtttacaaag 3900

cggttaagct gggatgggtg catacgtggg gatatgagat gcatcttgga ctgtattttt 3960

aggttggcta tgttcccagc catatccctc cggggattca tgttgtgcag aaccaccagc 4020

acagtgtatc cggtgcactt gggaaatttg tcatgtagct tagaaggaaa tgcgtggaag 4080

aacttggaga cgcccttgtg acctccaaga ttttccatgc attcgtccat aatgatggca 4140

atgggcccac gggcggcggc ctgggcgaag atatttctgg gatcactaac gtcatagttg 4200

tgttccagga tgagatcgtc ataggccatt tttacaaagc gcgggcggag ggtgccagac 4260

tgcggtataa tggttccatc cggcccaggg gcgtagttac cctcacagat ttgcatttcc 4320

cacgctttga gttcagatgg ggggatcatg tctacctgcg gggcgatgaa gaaaacggtt 4380

tccggggtag gggagatcag ctgggaagaa agcaggttcc tgagcagctg cgacttaccg 4440

cagccggtgg gcccgtaaat cacacctatt accggctgca actggtagtt aagagagctg 4500

cagctgccgt catccctgag caggggggcc acttcgttaa gcatgtccct gactcgcatg 4560

ttttccctga ccaaatccgc cagaaggcgc tcgccgccca gcgatagcag ttcttgcaag 4620

gaagcaaagt ttttcaacgg tttgagaccg tccgccgtag gcatgctttt gagcgtttga 4680

ccaagcagtt ccaggcggtc ccacagctcg gtcacctgct ctacggcatc tcgatccagc 4740

atatctcctc gtttcgcggg ttggggcggc tttcgctgta cggcagtagt cggtgctcgt 4800

ccagacgggc cagggtcatg tctttccacg ggcgcagggt cctcgtcagc gtagtctggg 4860

tcacggtgaa ggggtgcgct ccgggctgcg cgctggccag ggtgcgcttg aggctggtcc 4920

tgctggtgct gaagcgctgc cggtcttcgc cctgcgcgtc ggccaggtag catttgacca 4980

tggtgtcata gtccagcccc tccgcggcgt ggcccttggc gcgcagcttg cccttggagg 5040

aggcgccgca cgaggggcag tgcagacttt tgagggcgta gagcttgggc gcgagaaata 5100

ccgattccgg ggagtaggca tccgcgccgc aggccccgca gacggtctcg cattccacga 5160

gccaggtgag ctctggccgt tcggggtcaa aaaccaggtt tcccccatgc tttttgatgc 5220

gtttcttacc tctggtttcc atgagccggt gtccacgctc ggtgacgaaa aggctgtccg 5280

tgtccccgta tacagacttg agaggcctgt cctcgagcgg tgttccgcgg tcctcctcgt 5340

atagaaactc ggaccactct gagacaaagg ctcgcgtcca ggccagcacg aaggaggcta 5400

agtgggaggg gtagcggtcg ttgtccacta gggggtccac tcgctccagg gtgtgaagac 5460

acatgtcgcc ctcttcggca tcaaggaagg tgattggttt gtaggtgtag gccacgtgac 5520

cgggtgttcc tgaagggggg ctataaaagg gggtgggggc gcgttcgtcc tcactctctt 5580

ccgcatcgct gtctgcgagg gccagctgtt ggggtgagta ctccctctga aaagcgggca 5640

tgacttctgc gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc 5700

ccgcggtgat gcctttgagg gtggccgcat ccatctggtc agaaaagaca atctttttgt 5760

tgtcaagctt ggtggcaaac gacccgtaga gggcgttgga cagcaacttg gcgatggagc 5820

gcagggtttg gtttttgtcg cgatcggcgc gctccttggc cgcgatgttt agctgcacgt 5880

attcgcgcgc aacgcaccgc cattcgggaa agacggtggt gcgctcgtcg ggcaccaggt 5940

gcacgcgcca accgcggttg tgcagggtga caaggtcaac gctggtggct acctctccgc 6000

gtaggcgctc gttggtccag cagaggcggc cgcccttgcg cgagcagaat ggcggtaggg 6060

ggtctagctg cgtctcgtcc ggggggtctg cgtccacggt aaagaccccg ggcagcaggc 6120

gcgcgtcgaa gtagtctatc ttgcatcctt gcaagtctag cgcctgctgc catgcgcggg 6180

cggcaagcgc gcgctcgtat gggttgagtg ggggacccca tggcatgggg tgggtgagcg 6240

cggaggcgta catgccgcaa atgtcgtaaa cgtagagggg ctctctgagt attccaagat 6300

atgtagggta gcatcttcca ccgcggatgc tggcgcgcac gtaatcgtat agttcgtgcg 6360

agggagcgag gaggtcggga ccgaggttgc tacgggcggg ctgctctgct cggaagacta 6420

tctgcctgaa gatggcatgt gagttggatg atatggttgg acgctggaag acgttgaagc 6480

tggcgtctgt gagacctacc gcgtcacgca cgaaggaggc gtaggagtcg cgcagcttgt 6540

tgaccagctc ggcggtgacc tgcacgtcta gggcgcagta gtccagggtt tccttgatga 6600

tgtcatactt atcctgtccc ttttttttcc acagctcgcg gttgaggaca aactcttcgc 6660

ggtctttcca gtactcttgg atcggaaacc cgtcggcctc cgaacggtaa gagcctagca 6720

tgtagaactg gttgacggcc tggtaggcgc agcatccctt ttctacgggt agcgcgtatg 6780

cctgcgcggc cttccggagc gaggtgtggg tgagcgcaaa ggtgtccctg accatgactt 6840

tgaggtactg gtatttgaag tcagtgtcgt cgcatccgcc ctgctcccag agcaaaaagt 6900

ccgtgcgctt tttggaacgc ggatttggca gggcgaaggt gacatcgttg aagagtatct 6960

ttcccgcgcg aggcataaag ttgcgtgtga tgcggaaggg tcccggcacc tcggaacggt 7020

tgttaattac ctgggcggcg agcacgatct cgtcaaagcc gttgatgttg tggcccacaa 7080

tgtaaagttc caagaagcgc gggatgccct tgatggaagg caatttttta agttcctcgt 7140

aggtgagctc ttcaggggag ctgagcccgt gctctgaaag ggcccagtct gcaagatgag 7200

ggttggaagc gacgaatgag ctccacaggt cacgggccat tagcatttgc aggtggtcgc 7260

gaaaggtcct aaactggcga cctatggcca ttttttctgg ggtgatgcag tagaaggtaa 7320

gcgggtcttg ttcccagcgg tcccatccaa ggttcgcggc taggtctcgc gcggcagtca 7380

ctagaggctc atctccgccg aacttcatga ccagcatgaa gggcacgagc tgcttcccaa 7440

aggcccccat ccaagtatag gtctctacat cgtaggtgac aaagagacgc tcggtgcgag 7500

gatgcgagcc gatcgggaag aactggatct cccgccacca attggaggag tggctattga 7560

tgtggtgaaa gtagaagtcc ctgcgacggg ccgaacactc gtgctggctt ttgtaaaaac 7620

gtgcgcagta ctggcagcgg tgcacgggct gtacatcctg cacgaggttg acctgacgac 7680

cgcgcacaag gaagcagagt gggaatttga gcccctcgcc tggcgggttt ggctggtggt 7740

cttctacttc ggctgcttgt ccttgaccgt ctggctgctc gaggggagtt acggtggatc 7800

ggaccaccac gccgcgcgag cccaaagtcc agatgtccgc gcgcggcggt cggagcttga 7860

tgacaacatc gcgcagatgg gagctgtcca tggtctggag ctcccgcggc gtcaggtcag 7920

gcgggagctc ctgcaggttt acctcgcata gacgggtcag ggcgcgggct agatccaggt 7980

gatacctaat ttccaggggc tggttggtgg cggcgtcgat ggcttgcaag aggccgcatc 8040

cccgcggcgc gactacggta ccgcgcggcg ggcggtgggc cgcgggggtg tccttggatg 8100

atgcatctaa aagcggtgac gcgggcgagc ccccggaggt agggggggct ccggacccgc 8160

cgggagaggg ggcaggggca cgtcggcgcc gcgcgcgggc aggagctggt gctgcgcgcg 8220

taggttgctg gcgaacgcga cgacgcggcg gttgatctcc tgaatctggc gcctctgcgt 8280

gaagacgacg ggcccggtga gcttgaacct gaaagagagt tcgacagaat caatttcggt 8340

gtcgttgacg gcggcctggc gcaaaatctc ctgcacgtct cctgagttgt cttgataggc 8400

gatctcggcc atgaactgct cgatctcttc ctcctggaga tctccgcgtc cggctcgctc 8460

cacggtggcg gcgaggtcgt tggaaatgcg ggccatgagc tgcgagaagg cgttgaggcc 8520

tccctcgttc cagacgcggc tgtagaccac gcccccttcg gcatcgcggg cgcgcatgac 8580

cacctgcgcg agattgagct ccacgtgccg ggcgaagacg gcgtagtttc gcaggcgctg 8640

aaagaggtag ttgagggtgg tggcggtgtg ttctgccacg aagaagtaca taacccagcg 8700

tcgcaacgtg gattcgttga tatcccccaa ggcctcaagg cgctccatgg cctcgtagaa 8760

gtccacggcg aagttgaaaa actgggagtt gcgcgccgac acggttaact cctcctccag 8820

aagacggatg agctcggcga cagtgtcgcg cacctcgcgc tcaaaggcta caggggcctc 8880

ttcttcttct tcaatctcct cttccataag ggcctcccct tcttcttctt ctggcggcgg 8940

tgggggaggg gggacacggc ggcgacgacg gcgcaccggg aggcggtcga caaagcgctc 9000

gatcatctcc ccgcggcgac ggcgcatggt ctcggtgacg gcgcggccgt tctcgcgggg 9060

gcgcagttgg aagacgccgc ccgtcatgtc ccggttatgg gttggcgggg ggctgccatg 9120

cggcagggat acggcgctaa cgatgcatct caacaattgt tgtgtaggta ctccgccgcc 9180

gagggacctg agcgagtccg catcgaccgg atcggaaaac ctctcgagaa aggcgtctaa 9240

ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc ggcagcgggc ggcggtcggg 9300

gttgtttctg gcggaggtgc tgctgatgat gtaattaaag taggcggtct tgagacggcg 9360

gatggtcgac agaagcacca tgtccttggg tccggcctgc tgaatgcgca ggcggtcggc 9420

catgccccag gcttcgtttt gacatcggcg caggtctttg tagtagtctt gcatgagcct 9480

ttctaccggc acttcttctt ctccttcctc ttgtcctgca tctcttgcat ctatcgctgc 9540

ggcggcggcg gagtttggcc gtaggtggcg ccctcttcct cccatgcgtg tgaccccgaa 9600

gcccctcatc ggctgaagca gggctaggtc ggcgacaacg cgctcggcta atatggcctg 9660

ctgcacctgc gtgagggtag actggaagtc atccatgtcc acaaagcggt ggtatgcgcc 9720

cgtgttgatg gtgtaagtgc agttggccat aacggaccag ttaacggtct ggtgacccgg 9780

ctgcgagagc tcggtgtacc tgagacgcga gtaagccctc gagtcaaata cgtagtcgtt 9840

gcaagtccgc accaggtact ggtatcccac caaaaagtgc ggcggcggct ggcggtagag 9900

gggccagcgt agggtggccg gggctccggg ggcgagatct tccaacataa ggcgatgata 9960

tccgtagatg tacctggaca tccaggtgat gccggcggcg gtggtggagg cgcgcggaaa 10020

gtcgcggacg cggttccaga tgttgcgcag cggcaaaaag tgctccatgg tcgggacgct 10080

ctggccggtc aggcgcgcgc aatcgttgac gctctagacc gtgcaaaagg agagcctgta 10140

agcgggcact cttccgtggt ctggtggata aattcgcaag ggtatcatgg cggacgaccg 10200

gggttcgagc cccgtatccg gccgtccgcc gtgatccatg cggttaccgc ccgcgtgtcg 10260

aacccaggtg tgcgacgtca gacaacgggg gagtgctcct tttggcttcc ttccaggcgc 10320

ggcggctgct gcgctagctt ttttggccac tggccgcgcg cagcgtaagc ggttaggctg 10380

gaaagcgaaa gcattaagtg gctcgctccc tgtagccgga gggttatttt ccaagggttg 10440

agtcgcggga cccccggttc gagtctcgga ccggccggac tgcggcgaac gggggtttgc 10500

ctccccgtca tgcaagaccc cgcttgcaaa ttcctccgga aacagggacg agcccctttt 10560

ttgcttttcc cagatgcatc cggtgctgcg gcagatgcgc ccccctcctc agcagcggca 10620

agagcaagag cagcggcaga catgcagggc accctcccct cctcctaccg cgtcaggagg 10680

ggcgacatcc gcggttgacg cggcagcaga tggtgattac gaacccccgc ggcgccgggc 10740

ccggcactac ctggacttgg aggagggcga gggcctggcg cggctaggag cgccctctcc 10800

tgagcggcac ccaagggtgc agctgaagcg tgatacgcgt gaggcgtacg tgccgcggca 10860

gaacctgttt cgcgaccgcg agggagagga gcccgaggag atgcgggatc gaaagttcca 10920

cgcagggcgc gagctgcggc atggcctgaa tcgcgagcgg ttgctgcgcg aggaggactt 10980

tgagcccgac gcgcgaaccg ggattagtcc cgcgcgcgca cacgtggcgg ccgccgacct 11040

ggtaaccgca tacgagcaga cggtgaacca ggagattaac tttcaaaaaa gctttaacaa 11100

ccacgtgcgt acgcttgtgg cgcgcgagga ggtggctata ggactgatgc atctgtggga 11160

ctttgtaagc gcgctggagc aaaacccaaa tagcaagccg ctcatggcgc agctgttcct 11220

tatagtgcag cacagcaggg acaacgaggc attcagggat gcgctgctaa acatagtaga 11280

gcccgagggc cgctggctgc tcgatttgat aaacatcctg cagagcatag tggtgcagga 11340

gcgcagcttg agcctggctg acaaggtggc cgccatcaac tattccatgc ttagcctggg 11400

caagttttac gcccgcaaga tataccatac cccttacgtt cccatagaca aggaggtaaa 11460

gatcgagggg ttctacatgc gcatggcgct gaaggtgctt accttgagcg acgacctggg 11520

cgtttatcgc aacgagcgca tccacaaggc cgtgagcgtg agccggcggc gcgagctcag 11580

cgaccgcgag ctgatgcaca gcctgcaaag ggccctggct ggcacgggca gcggcgatag 11640

agaggccgag tcctactttg acgcgggcgc tgacctgcgc tgggccccaa gccgacgcgc 11700

cctggaggca gctggggccg gacctgggct ggcggtggca cccgcgcgcg ctggcaacgt 11760

cggcggcgtg gaggaatatg acgaggacga tgagtacgag ccagaggacg gcgagtacta 11820

agcggtgatg tttctgatca gatgatgcaa gacgcaacgg acccggcggt gcgggcggcg 11880

ctgcagagcc agccgtccgg ccttaactcc acggacgact ggcgccaggt catggaccgc 11940

atcatgtcgc tgactgcgcg caatcctgac gcgttccggc agcagccgca ggccaaccgg 12000

ctctccgcaa ttctggaagc ggtggtcccg gcgcgcgcaa accccacgca cgagaaggtg 12060

ctggcgatcg taaacgcgct ggccgaaaac agggccatcc ggcccgacga ggccggcctg 12120

gtctacgacg cgctgcttca gcgcgtggct cgttacaaca gcggcaacgt gcagaccaac 12180

ctggaccggc tggtggggga tgtgcgcgag gccgtggcgc agcgtgagcg cgcgcagcag 12240

cagggcaacc tgggctccat ggttgcacta aacgccttcc tgagtacaca gcccgccaac 12300

gtgccgcggg gacaggagga ctacaccaac tttgtgagcg cactgcggct aatggtgact 12360

gagacaccgc aaagtgaggt gtaccagtct gggccagact attttttcca gaccagtaga 12420

caaggcctgc agaccgtaaa cctgagccag gctttcaaaa acttgcaggg gctgtggggg 12480

gtgcgggctc ccacaggcga ccgcgcgacc gtgtctagct tgctgacgcc caactcgcgc 12540

ctgttgctgc tgctaatagc gcccttcacg gacagtggca gcgtgtcccg ggacacatac 12600

ctaggtcact tgctgacact gtaccgcgag gccataggtc aggcgcatgt ggacgagcat 12660

actttccagg agattacaag tgtcagccgc gcgctggggc aggaggacac gggcagcctg 12720

gaggcaaccc taaactacct gctgaccaac cggcggcaga agatcccctc gttgcacagt 12780

ttaaacagcg aggaggagcg cattttgcgc tacgtgcagc agagcgtgag ccttaacctg 12840

atgcgcgacg gggtaacgcc cagcgtggcg ctggacatga ccgcgcgcaa catggaaccg 12900

ggcatgtatg cctcaaaccg gccgtttatc aaccgcctaa tggactactt gcatcgcgcg 12960

gccgccgtga accccgagta tttcaccaat gccatcttga acccgcactg gctaccgccc 13020

cctggtttct acaccggggg attcgaggtg cccgagggta acgatggatt cctctgggac 13080

gacatagacg acagcgtgtt ttccccgcaa ccgcagaccc tgctagagtt gcaacagcgc 13140

gagcaggcag aggcggcgct gcgaaaggaa agcttccgca ggccaagcag cttgtccgat 13200

ctaggcgctg cggccccgcg gtcagatgct agtagcccat ttccaagctt gatagggtct 13260

cttaccagca ctcgcaccac ccgcccgcgc ctgctgggcg aggaggagta cctaaacaac 13320

tcgctgctgc agccgcagcg cgaaaaaaac ctgcctccgg catttcccaa caacgggata 13380

gagagcctag tggacaagat gagtagatgg aagacgtacg cgcaggagca cagggacgtg 13440

ccaggcccgc gcccgcccac ccgtcgtcaa aggcacgacc gtcagcgggg tctggtgtgg 13500

gaggacgatg actcggcaga cgacagcagc gtcctggatt tgggagggag tggcaacccg 13560

tttgcgcacc ttcgccccag gctggggaga atgttttaaa aaaaaaaaaa gcatgatgca 13620

aaataaaaaa ctcaccaagg ccatggcacc gagcgttggt tttcttgtat tccccttagt 13680

atgcggcgcg cggcgatgta tgaggaaggt cctcctccct cctacgagag tgtggtgagc 13740

gcggcgccag tggcggcggc gctgggttct cccttcgatg ctcccctgga cccgccgttt 13800

gtgcctccgc ggtacctgcg gcctaccggg gggagaaaca gcatccgtta ctctgagttg 13860

gcacccctat tcgacaccac ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca 13920

tccctgaact accagaacga ccacagcaac tttctgacca cggtcattca aaacaatgac 13980

tacagcccgg gggaggcaag cacacagacc atcaatcttg acgaccggtc gcactggggc 14040

ggcgacctga aaaccatcct gcataccaac atgccaaatg tgaacgagtt catgtttacc 14100

aataagttta aggcgcgggt gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag 14160

ctgaaatacg agtgggtgga gttcacgctg cccgagggca actactccga gaccatgacc 14220

atagacctta tgaacaacgc gatcgtggag cactacttga aagtgggcag acagaacggg 14280

gttctggaaa gcgacatcgg ggtaaagttt gacacccgca acttcagact ggggtttgac 14340

cccgtcactg gtcttgtcat gcctggggta tatacaaacg aagccttcca tccagacatc 14400

attttgctgc caggatgcgg ggtggacttc acccacagcc gcctgagcaa cttgttgggc 14460

atccgcaagc ggcaaccctt ccaggagggc tttaggatca cctacgatga tctggagggt 14520

ggtaacattc ccgcactgtt ggatgtggac gcctaccagg cgagcttgaa agatgacacc 14580

gaacagggcg ggggtggcgc aggcggcagc aacagcagtg gcagcggcgc ggaagagaac 14640

tccaacgcgg cagccgcggc aatgcagccg gtggaggaca tgaacgatca tgccattcgc 14700

ggcgacacct ttgccacacg ggctgaggag aagcgcgctg aggccgaagc agcggccgaa 14760

gctgccgccc ccgctgcgca acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa 14820

cccctgacag aggacagcaa gaaacgcagt tacaacctaa taagcaatga cagcaccttc 14880

acccagtacc gcagctggta ccttgcatac aactacggcg accctcagac cggaatccgc 14940

tcatggaccc tgctttgcac tcctgacgta acctgcggct cggagcaggt ctactggtcg 15000

ttgccagaca tgatgcaaga ccccgtgacc ttccgctcca cgcgccagat cagcaacttt 15060

ccggtggtgg gcgccgagct gttgcccgtg cactccaaga gcttctacaa cgaccaggcc 15120

gtctactccc aactcatccg ccagtttacc tctctgaccc acgtgttcaa tcgctttccc 15180

gagaaccaga ttttggcgcg cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt 15240

cctgctctca cagatcacgg gacgctaccg ctgcgcaaca gcatcggagg agtccagcga 15300

gtgaccatta ctgacgccag acgccgcacc tgcccctacg tttacaaggc cctgggcata 15360

gtctcgccgc gcgtcctatc gagccgcact ttttgagcaa gcatgtccat ccttatatcg 15420

cccagcaata acacaggctg gggcctgcgc ttcccaagca agatgtttgg cggggccaag 15480

aagcgctccg accaacaccc agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg 15540

cacaaacgcg gccgcactgg gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag 15600

gaggcgcgca actacacgcc cacgccgcca ccagtgtcca cagtggacgc ggccattcag 15660

accgtggtgc gcggagcccg gcgctatgct aaaatgaaga gacggcggag gcgcgtagca 15720

cgtcgccacc gccgccgacc cggcactgcc gcccaacgcg cggcggcggc cctgcttaac 15780

cgcgcacgtc gcaccggccg acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt 15840

attgtcactg tgccccccag gtccaggcga cgagcggccg ccgcagcagc cgcggccatt 15900

agtgctatga ctcagggtcg caggggcaac gtgtattggg tgcgcgactc ggttagcggc 15960

ctgcgcgtgc ccgtgcgcac ccgccccccg cgcaactaga ttgcaagaaa aaactactta 16020

gactcgtact gttgtatgta tccagcggcg gcggcgcgca acgaagctat gtccaagcgc 16080

aaaatcaaag aagagatgct ccaggtcatc gcgccggaga tctatggccc cccgaagaag 16140

gaagagcagg attacaagcc ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat 16200

gatgatgaac ttgacgacga ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta 16260

cagtggaaag gtcgacgcgt aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg 16320

cccggtgagc gctccacccg cacctacaag cgcgtgtatg atgaggtgta cggcgacgag 16380

gacctgcttg agcaggccaa cgagcgcctc ggggagtttg cctacggaaa gcggcataag 16440

gacatgctgg cgttgccgct ggacgagggc aacccaacac ctagcctaaa gcccgtaaca 16500

ctgcagcagg tgctgcccgc gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag 16560

tctggtgact tggcacccac cgtgcagctg atggtaccca agcgccagcg actggaagat 16620

gtcttggaaa aaatgaccgt ggaacctggg ctggagcccg aggtccgcgt gcggccaatc 16680

aagcaggtgg cgccgggact gggcgtgcag accgtggacg ttcagatacc cactaccagt 16740

agcaccagta ttgccaccgc cacagagggc atggagacac aaacgtcccc ggttgcctca 16800

gcggtggcgg atgccgcggt gcaggcggtc gctgcggccg cgtccaagac ctctacggag 16860

gtgcaaacgg acccgtggat gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg 16920

aagtacggcg ccgccagcgc gctactgccc gaatatgccc tacatccttc cattgcgcct 16980

acccccggct atcgtggcta cacctaccgc cccagaagac gagcaactac ccgacgccga 17040

accaccactg gaacccgccg ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc 17100

gtgcgcaggg tggctcgcga aggaggcagg accctggtgc tgccaacagc gcgctaccac 17160

cccagcatcg tttaaaagcc ggtctttgtg gttcttgcag atatggccct cacctgccgc 17220

ctccgtttcc cggtgccggg attccgagga agaatgcacc gtaggagggg catggccggc 17280

cacggcctga cgggcggcat gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt 17340

cgcatgcgcg gcggtatcct gcccctcctt attccactga tcgccgcggc gattggcgcc 17400

gtgcccggaa ttgcatccgt ggccttgcag gcgcagagac actgattaaa aacaagttgc 17460

atgtggaaaa atcaaaataa aaagtctgga ctctcacgct cgcttggtcc tgtaactatt 17520

ttgtagaatg gaagacatca actttgcgtc tctggccccg cgacacggct cgcgcccgtt 17580

catgggaaac tggcaagata tcggcaccag caatatgagc ggtggcgcct tcagctgggg 17640

ctcgctgtgg agcggcatta aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc 17700

ctggaacagc agcacaggcc agatgctgag ggataagttg aaagagcaaa atttccaaca 17760

aaaggtggta gatggcctgg cctctggcat tagcggggtg gtggacctgg ccaaccaggc 17820

agtgcaaaat aagattaaca gtaagcttga tccccgccct cccgtagagg agcctccacc 17880

ggccgtggag acagtgtctc cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga 17940

agaaactctg gtgacgcaaa tagacgagcc tccctcgtac gaggaggcac taaagcaagg 18000

cctgcccacc acccgtccca tcgcgcccat ggctaccgga gtgctgggcc agcacacacc 18060

cgtaacgctg gacctgcctc cccccgccga cacccagcag aaacctgtgc tgccaggccc 18120

gaccgccgtt gttgtaaccc gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc 18180

gcgatcgttg cggcccgtag ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg 18240

tctgggggtg caatccctga agcgccgacg atgcttctga tagctaacgt gtcgtatgtg 18300

tgtcatgtat gcgtccatgt cgccgccaga ggagctgctg agccgccgcg cgcccgcttt 18360

ccaagatggc taccccttcg atgatgccgc agtggtctta catgcacatc tcgggccagg 18420

acgcctcgga gtacctgagc cccgggctgg tgcagtttgc ccgcgccacc gagacgtact 18480

tcagcctgaa taacaagttt agaaacccca cggtggcgcc tacgcacgac gtgaccacag 18540

accggtccca gcgtttgacg ctgcggttca tccctgtgga ccgtgaggat actgcgtact 18600

cgtacaaggc gcggttcacc ctagctgtgg gtgataaccg tgtgctggac atggcttcca 18660

cgtactttga catccgcggc gtgctggaca ggggccctac ttttaagccc tactctggca 18720

ctgcctacaa cgccctggct cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg 18780

ctactgctct tgaaataaac ctagaagaag aggacgatga caacgaagac gaagtagacg 18840

agcaagctga gcagcaaaaa actcacgtat ttgggcaggc gccttattct ggtataaata 18900

ttacaaagga gggtattcaa ataggtgtcg aaggtcaaac acctaaatat gccgataaaa 18960

catttcaacc tgaacctcaa ataggagaat ctcagtggta cgaaacagaa attaatcatg 19020

cagctgggag agtcctaaaa aagactaccc caatgaaacc atgttacggt tcatatgcaa 19080

aacccacaaa tgaaaatgga gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag 19140

aaagtcaagt ggaaatgcaa tttttctcaa ctactgaggc agccgcaggc aatggtgata 19200

acttgactcc taaagtggta ttgtacagtg aagatgtaga tatagaaacc ccagacactc 19260

atatttctta catgcccact attaaggaag gtaactcacg agaactaatg ggccaacaat 19320

ctatgcccaa caggcctaat tacattgctt ttagggacaa ttttattggt ctaatgtatt 19380

acaacagcac gggtaatatg ggtgttctgg cgggccaagc atcgcagttg aatgctgttg 19440

tagatttgca agacagaaac acagagcttt cataccagct tttgcttgat tccattggtg 19500

atagaaccag gtacttttct atgtggaatc aggctgttga cagctatgat ccagatgtta 19560

gaattattga aaatcatgga actgaagatg aacttccaaa ttactgcttt ccactgggag 19620

gtgtgattaa tacagagact cttaccaagg taaaacctaa aacaggtcag gaaaatggat 19680

gggaaaaaga tgctacagaa ttttcagata aaaatgaaat aagagttgga aataattttg 19740

ccatggaaat caatctaaat gccaacctgt ggagaaattt cctgtactcc aacatagcgc 19800

tgtatttgcc cgacaagcta aagtacagtc cttccaacgt aaaaatttct gataacccaa 19860

acacctacga ctacatgaac aagcgagtgg tggctcccgg gctagtggac tgctacatta 19920

accttggagc acgctggtcc cttgactata tggacaacgt caacccattt aaccaccacc 19980

gcaatgctgg cctgcgctac cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc 20040

acatccaggt gcctcagaag ttctttgcca ttaaaaacct ccttctcctg ccgggctcat 20100

acacctacga gtggaacttc aggaaggatg ttaacatggt tctgcagagc tccctaggaa 20160

atgacctaag ggttgacgga gccagcatta agtttgatag catttgcctt tacgccacct 20220

tcttccccat ggcccacaac accgcctcca cgcttgaggc catgcttaga aacgacacca 20280

acgaccagtc ctttaacgac tatctctccg ccgccaacat gctctaccct atacccgcca 20340

acgctaccaa cgtgcccata tccatcccct cccgcaactg ggcggctttc cgcggctggg 20400

ccttcacgcg ccttaagact aaggaaaccc catcactggg ctcgggctac gacccttatt 20460

acacctactc tggctctata ccctacctag atggaacctt ttacctcaac cacaccttta 20520

agaaggtggc cattaccttt gactcttctg tcagctggcc tggcaatgac cgcctgctta 20580

cccccaacga gtttgaaatt aagcgctcag ttgacgggga gggttacaac gttgcccagt 20640

gtaacatgac caaagactgg ttcctggtac aaatgctagc taactataac attggctacc 20700

agggcttcta tatcccagag agctacaagg accgcatgta ctccttcttt agaaacttcc 20760

agcccatgag ccgtcaggtg gtggatgata ctaaatacaa ggactaccaa caggtgggca 20820

tcctacacca acacaacaac tctggatttg ttggctacct tgcccccacc atgcgcgaag 20880

gacaggccta ccctgctaac ttcccctatc cgcttatagg caagaccgca gttgacagca 20940

ttacccagaa aaagtttctt tgcgatcgca ccctttggcg catcccattc tccagtaact 21000

ttatgtccat gggcgcactc acagacctgg gccaaaacct tctctacgcc aactccgccc 21060

acgcgctaga catgactttt gaggtggatc ccatggacga gcccaccctt ctttatgttt 21120

tgtttgaagt ctttgacgtg gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg 21180

tgtacctgcg cacgcccttc tcggccggca acgccacaac ataaagaagc aagcaacatc 21240

aacaacagct gccgccatgg gctccagtga gcaggaactg aaagccattg tcaaagatct 21300

tggttgtggg ccatattttt tgggcaccta tgacaagcgc tttccaggct ttgtttctcc 21360

acacaagctc gcctgcgcca tagtcaatac ggccggtcgc gagactgggg gcgtacactg 21420

gatggccttt gcctggaacc cgcactcaaa aacatgctac ctctttgagc cctttggctt 21480

ttctgaccag cgactcaagc aggtttacca gtttgagtac gagtcactcc tgcgccgtag 21540

cgccattgct tcttcccccg accgctgtat aacgctggaa aagtccaccc aaagcgtaca 21600

ggggcccaac tcggccgcct gtggactatt ctgctgcatg tttctccacg cctttgccaa 21660

ctggccccaa actcccatgg atcacaaccc caccatgaac cttattaccg gggtacccaa 21720

ctccatgctc aacagtcccc aggtacagcc caccctgcgt cgcaaccagg aacagctcta 21780

cagcttcctg gagcgccact cgccctactt ccgcagccac agtgcgcaga ttaggagcgc 21840

cacttctttt tgtcacttga aaaacatgta aaaataatgt actagagaca ctttcaataa 21900

aggcaaatgc ttttatttgt acactctcgg gtgattattt acccccaccc ttgccgtctg 21960

cgccgtttaa aaatcaaagg ggttctgccg cgcatcgcta tgcgccactg gcagggacac 22020

gttgcgatac tggtgtttag tgctccactt aaactcaggc acaaccatcc gcggcagctc 22080

ggtgaagttt tcactccaca ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc 22140

cgatatcttg aagtcgcagt tggggcctcc gccctgcgcg cgcgagttgc gatacacagg 22200

gttgcagcac tggaacacta tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc 22260

ggagatcaga tccgcgtcca ggtcctccgc gttgctcagg gcgaacggag tcaactttgg 22320

tagctgcctt cccaaaaagg gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg 22380

catcaaaagg tgaccgtgcc cggtctgggc gttaggatac agcgcctgca taaaagcctt 22440

gatctgctta aaagccacct gagcctttgc gccttcagag aagaacatgc cgcaagactt 22500

gccggaaaac tgattggccg gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt 22560

ggagatctgc accacatttc ggccccaccg gttcttcacg atcttggcct tgctagactg 22620

ctccttcagc gcgcgctgcc cgttttcgct cgtcacatcc atttcaatca cgtgctcctt 22680

atttatcata atgcttccgt gtagacactt aagctcgcct tcgatctcag cgcagcggtg 22740

cagccacaac gcgcagcccg tgggctcgtg atgcttgtag gtcacctctg caaacgactg 22800

caggtacgcc tgcaggaatc gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt 22860

cagctgcaac ccgcggtgct cctcgttcag ccaggtcttg catacggccg ccagagcttc 22920

cacttggtca ggcagtagtt tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc 22980

catcagcgcg cgcgcagcct ccatgccctt ctcccacgca gacacgatcg gcacactcag 23040

cgggttcatc accgtaattt cactttccgc ttcgctgggc tcttcctctt cctcttgcgt 23100

ccgcatacca cgcgccactg ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc 23160

tttgccatgc ttgattagca ccggtgggtt gctgaaaccc accatttgta gcgccacatc 23220

ttctctttct tcctcgctgt ccacgattac ctctggtgat ggcgggcgct cgggcttggg 23280

agaagggcgc ttctttttct tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg 23340

ccgcgggctg ggtgtgcgcg gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga 23400

ctcgatacgc cgcctcatcc gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga 23460

cggggacgac acgtcctcca tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg 23520

ggtggtttcg cgctgctcct cttcccgact ggccatttcc ttctcctata ggcagaaaaa 23580

gatcatggag tcagtcgaga agaaggacag cctaaccgcc ccctctgagt tcgccaccac 23640

cgcctccacc gatgccgcca acgcgcctac caccttcccc gtcgaggcac ccccgcttga 23700

ggaggaggaa gtgattatcg agcaggaccc aggttttgta agcgaagacg acgaggaccg 23760

ctcagtacca acagaggata aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca 23820

agtcgggcgg ggggacgaaa ggcatggcga ctacctagat gtgggagacg acgtgctgtt 23880

gaagcatctg cagcgccagt gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt 23940

gcccctcgcc atagcggatg tcagccttgc ctacgaacgc cacctattct caccgcgcgt 24000

accccccaaa cgccaagaaa acggcacatg cgagcccaac ccgcgcctca acttctaccc 24060

cgtatttgcc gtgccagagg tgcttgccac ctatcacatc tttttccaaa actgcaagat 24120

acccctatcc tgccgtgcca accgcagccg agcggacaag cagctggcct tgcggcaggg 24180

cgctgtcata cctgatatcg cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg 24240

acgcgacgag aagcgcgcgg caaacgctct gcaacaggaa aacagcgaaa atgaaagtca 24300

ctctggagtg ttggtggaac tcgagggtga caacgcgcgc ctagccgtac taaaacgcag 24360

catcgaggtc acccactttg cctacccggc acttaaccta ccccccaagg tcatgagcac 24420

agtcatgagt gagctgatcg tgcgccgtgc gcagcccctg gagagggatg caaatttgca 24480

agaacaaaca gaggagggcc tacccgcagt tggcgacgag cagctagcgc gctggcttca 24540

aacgcgcgag cctgccgact tggaggagcg acgcaaacta atgatggccg cagtgctcgt 24600

taccgtggag cttgagtgca tgcagcggtt ctttgctgac ccggagatgc agcgcaagct 24660

agaggaaaca ttgcactaca cctttcgaca gggctacgta cgccaggcct gcaagatctc 24720

caacgtggag ctctgcaacc tggtctccta ccttggaatt ttgcacgaaa accgccttgg 24780

gcaaaacgtg cttcattcca cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg 24840

cgtttactta tttctatgct acacctggca gacggccatg ggcgtttggc agcagtgctt 24900

ggaggagtgc aacctcaagg agctgcagaa actgctaaag caaaacttga aggacctatg 24960

gacggccttc aacgagcgct ccgtggccgc gcacctggcg gacatcattt tccccgaacg 25020

cctgcttaaa accctgcaac agggtctgcc agacttcacc agtcaaagca tgttgcagaa 25080

ctttaggaac tttatcctag agcgctcagg aatcttgccc gccacctgct gtgcacttcc 25140

tagcgacttt gtgcccatta agtaccgcga atgccctccg ccgctttggg gccactgcta 25200

ccttctgcag ctagccaact accttgccta ccactctgac ataatggaag acgtgagcgg 25260

tgacggtcta ctggagtgtc actgtcgctg caacctatgc accccgcacc gctccctggt 25320

ttgcaattcg cagctgctta acgaaagtca aattatcggt acctttgagc tgcagggtcc 25380

ctcgcctgac gaaaagtccg cggctccggg gttgaaactc actccggggc tgtggacgtc 25440

ggcttacctt cgcaaatttg tacctgagga ctaccacgcc cacgagatta ggttctacga 25500

agaccaatcc cgcccgccta atgcggagct taccgcctgc gtcattaccc agggccacat 25560

tcttggccaa ttgcaagcca tcaacaaagc ccgccaagag tttctgctac gaaagggacg 25620

gggggtttac ttggaccccc agtccggcga ggagctcaac ccaatccccc cgccgccgca 25680

gccctatcag cagcagccgc gggcccttgc ttcccaggat ggcacccaaa aagaagctgc 25740

agctgccgcc gccacccacg gacgaggagg aatactggga cagtcaggca gaggaggttt 25800

tggacgagga ggaggaggac atgatggaag actgggagag cctagacgag gaagcttccg 25860

aggtcgaaga ggtgtcagac gaaacaccgt caccctcggt cgcattcccc tcgccggcgc 25920

cccagaaatc ggcaaccggt tccagcatgg ctacaacctc cgctcctcag gcgccgccgg 25980

cactgcccgt tcgccgaccc aaccgtagat gggacaccac tggaaccagg gccggtaagt 26040

ccaagcagcc gccgccgtta gcccaagagc aacaacagcg ccaaggctac cgctcatggc 26100

gcgggcacaa gaacgccata gttgcttgct tgcaagactg tgggggcaac atctccttcg 26160

cccgccgctt tcttctctac catcacggcg tggccttccc ccgtaacatc ctgcattact 26220

accgtcatct ctacagccca tactgcaccg gcggcagcgg cagcaacagc agcggccaca 26280

cagaagcaaa ggcgaccgga tagcaagact ctgacaaagc ccaagaaatc cacagcggcg 26340

gcagcagcag gaggaggagc gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag 26400

cttagaaaca ggatttttcc cactctgtat gctatatttc aacagagcag gggccaagaa 26460

caagagctga aaataaaaaa caggtctctg cgatccctca cccgcagctg cctgtatcac 26520

aaaagcgaag atcagcttcg gcgcacgctg gaagacgcgg aggctctctt cagtaaatac 26580

tgcgcgctga ctcttaagga ctagtttcgc gccctttctc aaatttaagc gcgaaaacta 26640

cgtcatctcc agcggccaca cccggcgcca gcacctgttg tcagcgccat tatgagcaag 26700

gaaattccca cgccctacat gtggagttac cagccacaaa tgggacttgc ggctggagct 26760

gcccaagact actcaacccg aataaactac atgagcgcgg gaccccacat gatatcccgg 26820

gtcaacggaa tacgcgccca ccgaaaccga attctcctgg aacaggcggc tattaccacc 26880

acacctcgta ataaccttaa tccccgtagt tggcccgctg ccctggtgta ccaggaaagt 26940

cccgctccca ccactgtggt acttcccaga gacgcccagg ccgaagttca gatgactaac 27000

tcaggggcgc agcttgcggg cggctttcgt cacagggtgc ggtcgcccgg gcagggtata 27060

actcacctga caatcagagg gcgaggtatt cagctcaacg acgagtcggt gagctcctcg 27120

cttggtctcc gtccggacgg gacatttcag atcggcggcg ccggccgctc ttcattcacg 27180

cctcgtcagg caatcctaac tctgcagacc tcgtcctctg agccgcgctc tggaggcatt 27240

ggaactctgc aatttattga ggagtttgtg ccatcggtct actttaaccc cttctcggga 27300

cctcccggcc actatccgga tcaatttatt cctaactttg acgcggtaaa ggactcggcg 27360

gacggctacg actgaatgtt aagtggagag gcagagcaac tgcgcctgaa acacctggtc 27420

cactgtcgcc gccacaagtg ctttgcccgc gactccggtg agttttgcta ctttgaattg 27480

cccgaggatc atatcgaggg cccggcgcac ggcgtccggc ttaccgccca gggagagctt 27540

gcccgtagcc tgattcggga gtttacccag cgccccctgc tagttgagcg ggacagggga 27600

ccctgtgttc tcactgtgat ttgcaactgt cctaaccctg gattacatca agatctttgt 27660

tgccatctct gtgctgagta taataaatac agaaattaaa atatactggg gctcctatcg 27720

ccatcctgta aacgccaccg tcttcacccg cccaagcaaa ccaaggcgaa ccttacctgg 27780

tacttttaac atctctccct ctgtgattta caacagtttc aacccagacg gagtgagtct 27840

acgagagaac ctctccgagc tcagctactc catcagaaaa aacaccaccc tccttacctg 27900

ccgggaacgt acgagtgcgt caccggccgc tgcaccacac ctaccgcctg accgtaaacc 27960

agactttttc cggacagacc tcaataactc tgtttaccag aacaggaggt gagcttagaa 28020

aacccttagg gtattaggcc aaaggcgcag ctactgtggg gtttatgaac aattcaagca 28080

actctacggg ctattctaat tcaggtttct ctagaatcgg ggttggggtt attctctgtc 28140

ttgtgattct ctttattctt atactaacgc ttctctgcct aaggctcgcc gcctgctgtg 28200

tgcacatttg catttattgt cagcttttta aacgctgggg tcgccaccca agatgattag 28260

gtacataatc ctaggtttac tcacccttgc gtcagcccac ggtaccaccc aaaaggtgga 28320

ttttaaggag ccagcctgta atgttacatt cgcagctgaa gctaatgagt gcaccactct 28380

tataaaatgc accacagaac atgaaaagct gcttattcgc cacaaaaaca aaattggcaa 28440

gtatgctgtt tatgctattt ggcagccagg tgacactaca gagtataatg ttacagtttt 28500

ccagggtaaa agtcataaaa cttttatgta tacttttcca ttttatgaaa tgtgcgacat 28560

taccatgtac atgagcaaac agtataagtt gtggccccca caaaattgtg tggaaaacac 28620

tggcactttc tgctgcactg ctatgctaat tacagtgctc gctttggtct gtaccctact 28680

ctatattaaa tacaaaagca gacgcagctt tattgaggaa aagaaaatgc cttaatttac 28740

taagttacaa agctaatgtc accactaact gctttactcg ctgcttgcaa aacaaattca 28800

aaaagttagc attataatta gaataggatt taaacccccc ggtcatttcc tgctcaatac 28860

cattcccctg aacaattgac tctatgtggg atatgctcca gcgctacaac cttgaagtca 28920

ggcttcctgg atgtcagcat ctgactttgg ccagcacctg tcccgcggat ttgttccagt 28980

ccaactacag cgacccaccc taacagagat gaccaacaca accaacgcgg ccgccgctac 29040

cggacttaca tctaccacaa atacacccca agtttctgcc tttgtcaata actgggataa 29100

cttgggcatg tggtggttct ccatagcgct tatgtttgta tgccttatta ttatgtggct 29160

catctgctgc ctaaagcgca aacgcgcccg accacccatc tatagtccca tcattgtgct 29220

acacccaaac aatgatggaa tccatagatt ggacggactg aaacacatgt tcttttctct 29280

tacagtatga ttaaatgaga catgattcct cgagttttta tattactgac ccttgttgcg 29340

cttttttgtg cgtgctccac attggctgcg gtttctcaca tcgaagtaga ctgcattcca 29400

gccttcacag tctatttgct ttacggattt gtcaccctca cgctcatctg cagcctcatc 29460

actgtggtca tcgcctttat ccagtgcatt gactgggtct gtgtgcgctt tgcatatctc 29520

agacaccatc cccagtacag ggacaggact atagctgagc ttcttagaat tctttaatta 29580

tgaaatttac tgtgactttt ctgctgatta tttgcaccct atctgcgttt tgttccccga 29640

cctccaagcc tcaaagacat atatcatgca gattcactcg tatatggaat attccaagtt 29700

gctacaatga aaaaagcgat ctttccgaag cctggttata tgcaatcatc tctgttatgg 29760

tgttctgcag taccatctta gccctagcta tatatcccta ccttgacatt ggctggaacg 29820

caatagatgc catgaaccac ccaactttcc ccgcgcccgc tatgcttcca ctgcaacaag 29880

ttgttgccgg cggctttgtc ccagccaatc agcctcgccc accttctccc acccccactg 29940

aaatcagcta ctttaatcta acaggaggag atgactgaca ccctagatct agaaatggac 30000

ggaattatta cagagcagcg cctgctagaa agacgcaggg cagcggccga gcaacagcgc 30060

atgaatcaag agctccaaga catggttaac ttgcaccagt gcaaaagggg tatcttttgt 30120

ctcgtaaagc aggccaaagt cacctacgac agtaatacca ccggacaccg ccttagctac 30180

aagttgccaa ccaagcgtca gaaattggtg gtcatggtgg gagaaaagcc cattaccata 30240

actcagcact cggtagaaac cgaaggctgc attcactcac cttgtcaagg acctgaggat 30300

ctctgcaccc ttattaagac cctgtgcggt ctcaaagatc ttattccctt taactaataa 30360

aaaaaaataa taaagcatca cttacttaaa atcagttagc aaatttctgt ccagtttatt 30420

cagcagcacc tccttgccct cctcccagct ctggtattgc agcttcctcc tggctgcaaa 30480

ctttctccac aatctaaatg gaatgtcagt ttcctcctgt tcctgtccat ccgcacccac 30540

tatcttcatg ttgttgcaga tgaagcgcgc aagaccgtct gaagatacct tcaaccccgt 30600

gtatccatat gacacggaaa ccggtcctcc aactgtgcct tttcttactc ctccctttgt 30660

atcccccaat gggtttcaag agagtccccc tggggtactc tctttgcgcc tatccgaacc 30720

tctagttacc tccaatggca tgcttgcgct caaaatgggc aacggcctct ctctggacga 30780

ggccggcaac cttacctccc aaaatgtaac cactgtgagc ccacctctca aaaaaaccaa 30840

gtcaaacata aacctggaaa tatctgcacc cctcacagtt acctcagaag ccctaactgt 30900

ggctgccgcc gcacctctaa tggtcgcggg caacacactc accatgcaat cacaggcccc 30960

gctaaccgtg cacgactcca aacttagcat tgccacccaa ggacccctca cagtgtcaga 31020

aggaaagcta gccctgcaaa catcaggccc cctcaccacc accgatagca gtacccttac 31080

tatcactgcc tcaccccctt taactactgc cactggtagc ttgggcattg acttgaaaga 31140

gcccatttat acacaaaatg gaaaactagg actaaagtac ggggctcctt tgcatgtaac 31200

agacgaccta aacactttga ccgtagcaac tggtccaggt gtgactatta ataatacttc 31260

cttgcaaact aaagttactg gagccttggg ttttgattca caaggcaata tgcaacttaa 31320

tgtagcagga ggactaagga ttgattctca aaacagacgc cttatacttg atgttagtta 31380

tccgtttgat gctcaaaacc aactaaatct aagactagga cagggccctc tttttataaa 31440

ctcagcccac aacttggata ttaactacaa caaaggcctt tacttgttta cagcttcaaa 31500

caattccaaa aagcttgagg ttaacctaag cactgccaag gggttgatgt ttgacgctac 31560

agccatagcc attaatgcag gagatgggct tgaatttggt tcacctaatg caccaaacac 31620

aaatcccctc aaaacaaaaa ttggccatgg cctagaattt gattcaaaca aggctatggt 31680

tcctaaacta ggaactggcc ttagttttga cagcacaggt gccattacag taggaaacaa 31740

aaataatgat aagctaactt tgtggaccac accagctcca tctcctaact gtagactaaa 31800

tgcagagaaa gatgctaaac tcactttggt cttaacaaaa tgtggcagtc aaatacttgc 31860

tacagtttca gttttggctg ttaaaggcag tttggctcca atatctggaa cagttcaaag 31920

tgctcatctt attataagat ttgacgaaaa tggagtgcta ctaaacaatt ccttcctgga 31980

cccagaatat tggaacttta gaaatggaga tcttactgaa ggcacagcct atacaaacgc 32040

tgttggattt atgcctaacc tatcagctta tccaaaatct cacggtaaaa ctgccaaaag 32100

taacattgtc agtcaagttt acttaaacgg agacaaaact aaacctgtaa cactaaccat 32160

tacactaaac ggtacacagg aaacaggaga cacaactcca agtgcatact ctatgtcatt 32220

ttcatgggac tggtctggcc acaactacat taatgaaata tttgccacat cctcttacac 32280

tttttcatac attgcccaag aataaagaat cgtttgtgtt atgtttcaac gtgtttattt 32340

ttcaattgca gaaaatttca agtcattttt cattcagtag tatagcccca ccaccacata 32400

gcttatacag atcaccgtac cttaatcaaa ctcacagaac cctagtattc aacctgccac 32460

ctccctccca acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca 32520

tatcatgggt aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca 32580

aacgctcatc agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt 32640

ccagctgctg agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag 32700

aagtccacgc ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct 32760

gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg 32820

cagtggtctc ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg 32880

cacagcagcg caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa 32940

tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag 33000

aacccacgtg gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca 33060

cgctggacat aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata 33120

taaacctctg attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct 33180

gcccgccggc tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg 33240

actcgtaacc atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca 33300

cgtgcataca cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa 33360

caacccattc ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca 33420

cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag 33480

cgcgggtttc tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca 33540

accgagatcg tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc 33600

ctgaagcaaa accaggtgcg ggcgtgacaa acagatctgc gtctccggtc tcgccgctta 33660

gatcgctctg tgtagtagtt gtagtatatc cactctctca aagcatccag gcgccccctg 33720

gcttcgggtt ctatgtaaac tccttcatgc gccgctgccc tgataacatc caccaccgca 33780

gaataagcca cacccagcca acctacacat tcgttctgcg agtcacacac gggaggagcg 33840

ggaagagctg gaagaaccat gttttttttt ttattccaaa agattatcca aaacctcaaa 33900

atgaagatct attaagtgaa cgcgctcccc tccggtggcg tggtcaaact ctacagccaa 33960

agaacagata atggcatttg taagatgttg cacaatggct tccaaaaggc aaacggccct 34020

cacgtccaag tggacgtaaa ggctaaaccc ttcagggtga atctcctcta taaacattcc 34080

agcaccttca accatgccca aataattctc atctcgccac cttctcaata tatctctaag 34140

caaatcccga atattaagtc cggccattgt aaaaatctgc tccagagcgc cctccacctt 34200

cagcctcaag cagcgaatca tgattgcaaa aattcaggtt cctcacagac ctgtataaga 34260

ttcaaaagcg gaacattaac aaaaataccg cgatcccgta ggtcccttcg cagggccagc 34320

tgaacataat cgtgcaggtc tgcacggacc agcgcggcca cttccccgcc aggaaccatg 34380

acaaaagaac ccacactgat tatgacacgc atactcggag ctatgctaac cagcgtagcc 34440

ccgatgtaag cttgttgcat gggcggcgat ataaaatgca aggtgctgct caaaaaatca 34500

ggcaaagcct cgcgcaaaaa agaaagcaca tcgtagtcat gctcatgcag ataaaggcag 34560

gtaagctccg gaaccaccac agaaaaagac accatttttc tctcaaacat gtctgcgggt 34620

ttctgcataa acacaaaata aaataacaaa aaaacattta aacattagaa gcctgtctta 34680

caacaggaaa aacaaccctt ataagcataa gacggactac ggccatgccg gcgtgaccgt 34740

aaaaaaactg gtcaccgtga ttaaaaagca ccaccgacag ctcctcggtc atgtccggag 34800

tcataatgta agactcggta aacacatcag gttgattcac atcggtcagt gctaaaaagc 34860

gaccgaaata gcccggggga atacataccc gcaggcgtag agacaacatt acagccccca 34920

taggaggtat aacaaaatta ataggagaga aaaacacata aacacctgaa aaaccctcct 34980

gcctaggcaa aatagcaccc tcccgctcca gaacaacata cagcgcttcc acagcggcag 35040

ccataacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac cactcgacac 35100

ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga gtatatatag 35160

gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa 35220

cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt 35280

tcccacgtta cgtcacttcc cattttaaga aaactacaat tcccaacaca tacaagttac 35340

tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg tcacaaactc 35400

caccccctca ttatcatatt ggcttcaatc caaaataagg tatattattg atgatgttaa 35460

ttaagaattc ggatctgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc 35520

ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga 35580

ccatcaggga cagcttcaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 35640

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 35700

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 35760

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 35820

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 35880

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 35940

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 36000

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 36060

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 36120

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 36180

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 36240

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 36300

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatc aatctaaagt 36360

atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 36420

gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 36480

atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 36540

ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 36600

cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 36660

agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 36720

cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 36780

tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 36840

agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 36900

gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 36960

gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 37020

ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 37080

tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 37140

tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 37200

gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 37260

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 37320

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 37380

gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 37440

tttcgtcttc aagaattgga tccgaattct taat 37474



7
37474
DNA
Artificial Sequence

pMRKAd5HIV-1 gag, non-coding


7

aagaattaat tgtagtagtt attatatgga ataaaaccta acttcggtta tactattact 60

cccccacctc aaacactgca ccgcgccccg cacccttgcc ccgcccactg catcatcaca 120

ccgccttcac actacaacgt tcacaccgcc ttgtgtacat tcgctgccta caccgttttc 180

actgcaaaaa ccacacgcgg ccacatgtgt ccttcactgt taaaagcgcg ccaaaatccg 240

cctacaacat catttaaacc cgcattggct cattctaaac cggtaaaagc gcccttttga 300

cttattctcc ttcactttag acttattaaa acacaatgag tatcgcgcat tataaacaga 360

tcccggcgcc cctgaaactg gcaaatgcac ctctgagcgg gtccacaaaa agagtccaca 420

aaaggcgcaa ggcccagttt caaccgcaaa ataataatat ccgccggcgc taggtaacgt 480

atgcaacata ggtatagtat tatacatgta aatataaccg agtacaggtt gtaatggcgg 540

tacaactgta actaataact gatcaataat tatcattagt taatgcccca gtaatcaagt 600

atcgggtata tacctcaagg cgcaatgtat tgaatgccat ttaccgggcg gaccgactgg 660

cgggttgctg ggggcgggta actgcagtta ttactgcata caagggtatc attgcggtta 720

tccctgaaag gtaactgcag ttacccacct cataaatgcc atttgacggg tgaaccgtca 780

tgtagttcac atagtatacg gttcatgcgg gggataactg cagttactgc catttaccgg 840

gcggaccgta atacgggtca tgtactggaa taccctgaaa ggatgaaccg tcatgtagat 900

gcataatcag tagcgataat ggtaccacta cgccaaaacc gtcatgtagt tacccgcacc 960

tatcgccaaa ctgagtgccc ctaaaggttc agaggtgggg taactgcagt taccctcaaa 1020

caaaaccgtg gttttagttg ccctgaaagg ttttacagca ttgttgaggc ggggtaactg 1080

cgtttacccg ccatccgcac atgccaccct ccagatatat tcgtctcgag caaatcactt 1140

ggcagtctag cggacctctg cggtaggtgc gacaaaactg gaggtatctt ctgtggccct 1200

ggctaggtcg gaggcgccgg cccttgccac gtaaccttgc gcctaagggg cacggttctc 1260

actctagatg gtacccacga tcccgaagac acgacagacc accactcgac ctgttcaccc 1320

tcttctagtc cgactccgga ccaccgttct tcttcatgtt cgatttcgtg taacacaccc 1380

ggaggtccct cgacctctcc aaacgacact tgggaccgga cgacctctgg agactcccca 1440

cgtccgtcta ggacccggtc gaggtcggga gggacgtttg tccgagactc ctcgactcca 1500

gggacatgtt gtgtcaccga tgggacatga cacacgtggt cttctaacta cacttcctgt 1560

ggttcctccg ggacctcttc taactcctcc tcgtcttgtt caggttcttc ttccgggtcg 1620

tccgacgacg accgtgtccg ttgaggtcgg tccacagggt cttgatgggg taacacgtct 1680

tggaggtccc ggtctaccac gtggtccggt agaggggggc ctgggactta cggacccact 1740

tccaccacct cctcttccgg aagaggggac tccactaggg gtacaagaga cgggacagac 1800

tcccacggtg gggggtcctg gacttgtggt acgacttgtg tcaccccccg gtagtccgac 1860

ggtacgtcta cgacttcctc tggtagttac tcctccgacg actcaccctg tccgacgtag 1920

gacacgtgcg accggggtaa cgggggccgg tctactccct cgggtccccg agactgtaac 1980

gaccgtggtg gaggtgggag gtcctcgtct aaccgaccta ctggttgttg ggggggtagg 2040

gacaccccct ttagatgttc tccacctagt aggacccgga cttgttctaa cactcctaca 2100

tgagggggtg gaggtaggac ctgtagtccg tcccggggtt cctcgggaag tccctgatac 2160

acctgtccaa gatgttctgg gactcccgac tcgtccggag ggtcctccac ttcttgacct 2220

actgtctctg ggacgaccac gtcttacggt tgggactgac gttctggtag gacttccggg 2280

acccgggacg acggtgggac ctcctctact actgtcggac ggtcccccac cccccgggac 2340

cagtgttccg gtcccacgac cgactccggt acagggtcca ctggttgagg cggtggtagt 2400

actacgtctc cccgttgaag tccttggtct ccttctgtca cttcacgaag ttgacaccgt 2460

tccacccggt gtaacggttc ttgacatccc gggggtcctt cttcccgacg accttcacac 2520

cgttcctccc ggtggtctac ttcctgacgt tactctccgt ccggttgaag gacccgtttt 2580

agaccgggag ggtgttcccg tccggaccgt tgaaggaggt caggtccgga ctcgggtgtc 2640

ggggagggct cctcaggaag tccaaacccc tcctcttctg gtgggggtcg gtcttcgtcc 2700

tcgggtaact gttcctcgac atgggggacc ggagggactc cagggacaaa ccgttgctgg 2760

ggaggagggt cattttattt cgggcccgtc tagacgacac ggaagatcaa cggtcggtag 2820

acaacaaacg gggagggggc acggaaggaa ctgggacctt ccacggtgag ggtgacagga 2880

aaggattatt ttactccttt aacgtagcgt aacagactca tccacagtaa gataagaccc 2940

cccaccccac cccgtcctgt cgttccccct cctaaccctt ctgttatcgt ccgtacgacc 3000

cctacgccac ccgagatacc ggctagccgc gcggcatgac tttacacacc cgcaccgaat 3060

tcccaccctt tcttatatat tccaccccca gaatacatca aaacatagac aaaacgtcgt 3120

cggcggcggc ggtactcgtg gttgagcaaa ctaccttcgt aacactcgag tataaactgt 3180

tgcgcgtacg ggggtacccg gccccacgca gtcttacact acccgaggtc gtaactacca 3240

gcggggcagg acgggcgttt gagatgatgg aactggatgc tctggcacag accttgcggc 3300

aacctctgac gtcggaggcg gcggcgaagt cggcgacgtc ggtggcgggc gccctaacac 3360

tgactgaaac gaaaggactc gggcgaacgt ttgtcacgtc gaagggcaag taggcgggcg 3420

ctactgttca actgccgaga aaaccgtgtt aacctaagaa actgggccct tgaattacag 3480

caaagagtcg tcgacaacct agacgcggtc gtccaaagac gggacttccg aaggagggga 3540

gggttacgcc aaattttgta tttatttttt ggtctgagac aaacctaaac ctagttcgtt 3600

cacagaacga cagaaataaa tccccaaaac gcgcgcgcca tccgggccct ggtcgccaga 3660

gccagcaact cccaggacac ataaaaaagg tcctgcacca tttccactga gacctacaag 3720

tctatgtacc cgtattcggg cagagacccc acctccatcg tggtgacgtc tcgaagtacg 3780

acgccccacc acaacatcta ctaggtcagc atcgtcctcg cgacccgcac cacggatttt 3840

tacagaaagt catcgttcga ctaacggtcc ccgtccggga accacattca caaatgtttc 3900

gccaattcga ccctacccac gtatgcaccc ctatactcta cgtagaacct gacataaaaa 3960

tccaaccgat acaagggtcg gtatagggag gcccctaagt acaacacgtc ttggtggtcg 4020

tgtcacatag gccacgtgaa ccctttaaac agtacatcga atcttccttt acgcaccttc 4080

ttgaacctct gcgggaacac tggaggttct aaaaggtacg taagcaggta ttactaccgt 4140

tacccgggtg cccgccgccg gacccgcttc tataaagacc ctagtgattg cagtatcaac 4200

acaaggtcct actctagcag tatccggtaa aaatgtttcg cgcccgcctc ccacggtctg 4260

acgccatatt accaaggtag gccgggtccc cgcatcaatg ggagtgtcta aacgtaaagg 4320

gtgcgaaact caagtctacc cccctagtac agatggacgc cccgctactt cttttgccaa 4380

aggccccatc ccctctagtc gacccttctt tcgtccaagg actcgtcgac gctgaatggc 4440

gtcggccacc cgggcattta gtgtggataa tggccgacgt tgaccatcaa ttctctcgac 4500

gtcgacggca gtagggactc gtccccccgg tgaagcaatt cgtacaggga ctgagcgtac 4560

aaaagggact ggtttaggcg gtcttccgcg agcggcgggt cgctatcgtc aagaacgttc 4620

cttcgtttca aaaagttgcc aaactctggc aggcggcatc cgtacgaaaa ctcgcaaact 4680

ggttcgtcaa ggtccgccag ggtgtcgagc cagtggacga gatgccgtag agctaggtcg 4740

tatagaggag caaagcgccc aaccccgccg aaagcgacat gccgtcatca gccacgagca 4800

ggtctgcccg gtcccagtac agaaaggtgc ccgcgtccca ggagcagtcg catcagaccc 4860

agtgccactt ccccacgcga ggcccgacgc gcgaccggtc ccacgcgaac tccgaccagg 4920

acgaccacga cttcgcgacg gccagaagcg ggacgcgcag ccggtccatc gtaaactggt 4980

accacagtat caggtcgggg aggcgccgca ccgggaaccg cgcgtcgaac gggaacctcc 5040

tccgcggcgt gctccccgtc acgtctgaaa actcccgcat ctcgaacccg cgctctttat 5100

ggctaaggcc cctcatccgt aggcgcggcg tccggggcgt ctgccagagc gtaaggtgct 5160

cggtccactc gagaccggca agccccagtt tttggtccaa agggggtacg aaaaactacg 5220

caaagaatgg agaccaaagg tactcggcca caggtgcgag ccactgcttt tccgacaggc 5280

acaggggcat atgtctgaac tctccggaca ggagctcgcc acaaggcgcc aggaggagca 5340

tatctttgag cctggtgaga ctctgtttcc gagcgcaggt ccggtcgtgc ttcctccgat 5400

tcaccctccc catcgccagc aacaggtgat cccccaggtg agcgaggtcc cacacttctg 5460

tgtacagcgg gagaagccgt agttccttcc actaaccaaa catccacatc cggtgcactg 5520

gcccacaagg acttcccccc gatattttcc cccacccccg cgcaagcagg agtgagagaa 5580

ggcgtagcga cagacgctcc cggtcgacaa ccccactcat gagggagact tttcgcccgt 5640

actgaagacg cgattctaac agtcaaaggt ttttgctcct cctaaactat aagtggaccg 5700

ggcgccacta cggaaactcc caccggcgta ggtagaccag tcttttctgt tagaaaaaca 5760

acagttcgaa ccaccgtttg ctgggcatct cccgcaacct gtcgttgaac cgctacctcg 5820

cgtcccaaac caaaaacagc gctagccgcg cgaggaaccg gcgctacaaa tcgacgtgca 5880

taagcgcgcg ttgcgtggcg gtaagccctt tctgccacca cgcgagcagc ccgtggtcca 5940

cgtgcgcggt tggcgccaac acgtcccact gttccagttg cgaccaccga tggagaggcg 6000

catccgcgag caaccaggtc gtctccgccg gcgggaacgc gctcgtctta ccgccatccc 6060

ccagatcgac gcagagcagg ccccccagac gcaggtgcca tttctggggc ccgtcgtccg 6120

cgcgcagctt catcagatag aacgtaggaa cgttcagatc gcggacgacg gtacgcgccc 6180

gccgttcgcg cgcgagcata cccaactcac cccctggggt accgtacccc acccactcgc 6240

gcctccgcat gtacggcgtt tacagcattt gcatctcccc gagagactca taaggttcta 6300

tacatcccat cgtagaaggt ggcgcctacg accgcgcgtg cattagcata tcaagcacgc 6360

tccctcgctc ctccagccct ggctccaacg atgcccgccc gacgagacga gccttctgat 6420

agacggactt ctaccgtaca ctcaacctac tataccaacc tgcgaccttc tgcaacttcg 6480

accgcagaca ctctggatgg cgcagtgcgt gcttcctccg catcctcagc gcgtcgaaca 6540

actggtcgag ccgccactgg acgtgcagat cccgcgtcat caggtcccaa aggaactact 6600

acagtatgaa taggacaggg aaaaaaaagg tgtcgagcgc caactcctgt ttgagaagcg 6660

ccagaaaggt catgagaacc tagcctttgg gcagccggag gcttgccatt ctcggatcgt 6720

acatcttgac caactgccgg accatccgcg tcgtagggaa aagatgccca tcgcgcatac 6780

ggacgcgccg gaaggcctcg ctccacaccc actcgcgttt ccacagggac tggtactgaa 6840

actccatgac cataaacttc agtcacagca gcgtaggcgg gacgagggtc tcgtttttca 6900

ggcacgcgaa aaaccttgcg cctaaaccgt cccgcttcca ctgtagcaac ttctcataga 6960

aagggcgcgc tccgtatttc aacgcacact acgccttccc agggccgtgg agccttgcca 7020

acaattaatg gacccgccgc tcgtgctaga gcagtttcgg caactacaac accgggtgtt 7080

acatttcaag gttcttcgcg ccctacggga actaccttcc gttaaaaaat tcaaggagca 7140

tccactcgag aagtcccctc gactcgggca cgagactttc ccgggtcaga cgttctactc 7200

ccaaccttcg ctgcttactc gaggtgtcca gtgcccggta atcgtaaacg tccaccagcg 7260

ctttccagga tttgaccgct ggataccggt aaaaaagacc ccactacgtc atcttccatt 7320

cgcccagaac aagggtcgcc agggtaggtt ccaagcgccg atccagagcg cgccgtcagt 7380

gatctccgag tagaggcggc ttgaagtact ggtcgtactt cccgtgctcg acgaagggtt 7440

tccgggggta ggttcatatc cagagatgta gcatccactg tttctctgcg agccacgctc 7500

ctacgctcgg ctagcccttc ttgacctaga gggcggtggt taacctcctc accgataact 7560

acaccacttt catcttcagg gacgctgccc ggcttgtgag cacgaccgaa aacatttttg 7620

cacgcgtcat gaccgtcgcc acgtgcccga catgtaggac gtgctccaac tggactgctg 7680

gcgcgtgttc cttcgtctca cccttaaact cggggagcgg accgcccaaa ccgaccacca 7740

gaagatgaag ccgacgaaca ggaactggca gaccgacgag ctcccctcaa tgccacctag 7800

cctggtggtg cggcgcgctc gggtttcagg tctacaggcg cgcgccgcca gcctcgaact 7860

actgttgtag cgcgtctacc ctcgacaggt accagacctc gagggcgccg cagtccagtc 7920

cgccctcgag gacgtccaaa tggagcgtat ctgcccagtc ccgcgcccga tctaggtcca 7980

ctatggatta aaggtccccg accaaccacc gccgcagcta ccgaacgttc tccggcgtag 8040

gggcgccgcg ctgatgccat ggcgcgccgc ccgccacccg gcgcccccac aggaacctac 8100

tacgtagatt ttcgccactg cgcccgctcg ggggcctcca tcccccccga ggcctgggcg 8160

gccctctccc ccgtccccgt gcagccgcgg cgcgcgcccg tcctcgacca cgacgcgcgc 8220

atccaacgac cgcttgcgct gctgcgccgc caactagagg acttagaccg cggagacgca 8280

cttctgctgc ccgggccact cgaacttgga ctttctctca agctgtctta gttaaagcca 8340

cagcaactgc cgccggaccg cgttttagag gacgtgcaga ggactcaaca gaactatccg 8400

ctagagccgg tacttgacga gctagagaag gaggacctct agaggcgcag gccgagcgag 8460

gtgccaccgc cgctccagca acctttacgc ccggtactcg acgctcttcc gcaactccgg 8520

agggagcaag gtctgcgccg acatctggtg cgggggaagc cgtagcgccc gcgcgtactg 8580

gtggacgcgc tctaactcga ggtgcacggc ccgcttctgc cgcatcaaag cgtccgcgac 8640

tttctccatc aactcccacc accgccacac aagacggtgc ttcttcatgt attgggtcgc 8700

agcgttgcac ctaagcaact atagggggtt ccggagttcc gcgaggtacc ggagcatctt 8760

caggtgccgc ttcaactttt tgaccctcaa cgcgcggctg tgccaattga ggaggaggtc 8820

ttctgcctac tcgagccgct gtcacagcgc gtggagcgcg agtttccgat gtccccggag 8880

aagaagaaga agttagagga gaaggtattc ccggagggga agaagaagaa gaccgccgcc 8940

accccctccc ccctgtgccg ccgctgctgc cgcgtggccc tccgccagct gtttcgcgag 9000

ctagtagagg ggcgccgctg ccgcgtacca gagccactgc cgcgccggca agagcgcccc 9060

cgcgtcaacc ttctgcggcg ggcagtacag ggccaatacc caaccgcccc ccgacggtac 9120

gccgtcccta tgccgcgatt gctacgtaga gttgttaaca acacatccat gaggcggcgg 9180

ctccctggac tcgctcaggc gtagctggcc tagccttttg gagagctctt tccgcagatt 9240

ggtcagtgtc agcgttccat ccgactcgtg gcaccgcccg ccgtcgcccg ccgccagccc 9300

caacaaagac cgcctccacg acgactacta cattaatttc atccgccaga actctgccgc 9360

ctaccagctg tcttcgtggt acaggaaccc aggccggacg acttacgcgt ccgccagccg 9420

gtacggggtc cgaagcaaaa ctgtagccgc gtccagaaac atcatcagaa cgtactcgga 9480

aagatggccg tgaagaagaa gaggaaggag aacaggacgt agagaacgta gatagcgacg 9540

ccgccgccgc ctcaaaccgg catccaccgc gggagaagga gggtacgcac actggggctt 9600

cggggagtag ccgacttcgt cccgatccag ccgctgttgc gcgagccgat tataccggac 9660

gacgtggacg cactcccatc tgaccttcag taggtacagg tgtttcgcca ccatacgcgg 9720

gcacaactac cacattcacg tcaaccggta ttgcctggtc aattgccaga ccactgggcc 9780

gacgctctcg agccacatgg actctgcgct cattcgggag ctcagtttat gcatcagcaa 9840

cgttcaggcg tggtccatga ccatagggtg gtttttcacg ccgccgccga ccgccatctc 9900

cccggtcgca tcccaccggc cccgaggccc ccgctctaga aggttgtatt ccgctactat 9960

aggcatctac atggacctgt aggtccacta cggccgccgc caccacctcc gcgcgccttt 10020

cagcgcctgc gccaaggtct acaacgcgtc gccgtttttc acgaggtacc agccctgcga 10080

gaccggccag tccgcgcgcg ttagcaactg cgagatctgg cacgttttcc tctcggacat 10140

tcgcccgtga gaaggcacca gaccacctat ttaagcgttc ccatagtacc gcctgctggc 10200

cccaagctcg gggcataggc cggcaggcgg cactaggtac gccaatggcg ggcgcacagc 10260

ttgggtccac acgctgcagt ctgttgcccc ctcacgagga aaaccgaagg aaggtccgcg 10320

ccgccgacga cgcgatcgaa aaaaccggtg accggcgcgc gtcgcattcg ccaatccgac 10380

ctttcgcttt cgtaattcac cgagcgaggg acatcggcct cccaataaaa ggttcccaac 10440

tcagcgccct gggggccaag ctcagagcct ggccggcctg acgccgcttg cccccaaacg 10500

gaggggcagt acgttctggg gcgaacgttt aaggaggcct ttgtccctgc tcggggaaaa 10560

aacgaaaagg gtctacgtag gccacgacgc cgtctacgcg gggggaggag tcgtcgccgt 10620

tctcgttctc gtcgccgtct gtacgtcccg tgggagggga ggaggatggc gcagtcctcc 10680

ccgctgtagg cgccaactgc gccgtcgtct accactaatg cttgggggcg ccgcggcccg 10740

ggccgtgatg gacctgaacc tcctcccgct cccggaccgc gccgatcctc gcgggagagg 10800

actcgccgtg ggttcccacg tcgacttcgc actatgcgca ctccgcatgc acggcgccgt 10860

cttggacaaa gcgctggcgc tccctctcct cgggctcctc tacgccctag ctttcaaggt 10920

gcgtcccgcg ctcgacgccg taccggactt agcgctcgcc aacgacgcgc tcctcctgaa 10980

actcgggctg cgcgcttggc cctaatcagg gcgcgcgcgt gtgcaccgcc ggcggctgga 11040

ccattggcgt atgctcgtct gccacttggt cctctaattg aaagtttttt cgaaattgtt 11100

ggtgcacgca tgcgaacacc gcgcgctcct ccaccgatat cctgactacg tagacaccct 11160

gaaacattcg cgcgacctcg ttttgggttt atcgttcggc gagtaccgcg tcgacaagga 11220

atatcacgtc gtgtcgtccc tgttgctccg taagtcccta cgcgacgatt tgtatcatct 11280

cgggctcccg gcgaccgacg agctaaacta tttgtaggac gtctcgtatc accacgtcct 11340

cgcgtcgaac tcggaccgac tgttccaccg gcggtagttg ataaggtacg aatcggaccc 11400

gttcaaaatg cgggcgttct atatggtatg gggaatgcaa gggtatctgt tcctccattt 11460

ctagctcccc aagatgtacg cgtaccgcga cttccacgaa tggaactcgc tgctggaccc 11520

gcaaatagcg ttgctcgcgt aggtgttccg gcactcgcac tcggccgccg cgctcgagtc 11580

gctggcgctc gactacgtgt cggacgtttc ccgggaccga ccgtgcccgt cgccgctatc 11640

tctccggctc aggatgaaac tgcgcccgcg actggacgcg acccggggtt cggctgcgcg 11700

ggacctccgt cgaccccggc ctggacccga ccgccaccgt gggcgcgcgc gaccgttgca 11760

gccgccgcac ctccttatac tgctcctgct actcatgctc ggtctcctgc cgctcatgat 11820

tcgccactac aaagactagt ctactacgtt ctgcgttgcc tgggccgcca cgcccgccgc 11880

gacgtctcgg tcggcaggcc ggaattgagg tgcctgctga ccgcggtcca gtacctggcg 11940

tagtacagcg actgacgcgc gttaggactg cgcaaggccg tcgtcggcgt ccggttggcc 12000

gagaggcgtt aagaccttcg ccaccagggc cgcgcgcgtt tggggtgcgt gctcttccac 12060

gaccgctagc atttgcgcga ccggcttttg tcccggtagg ccgggctgct ccggccggac 12120

cagatgctgc gcgacgaagt cgcgcaccga gcaatgttgt cgccgttgca cgtctggttg 12180

gacctggccg accaccccct acacgcgctc cggcaccgcg tcgcactcgc gcgcgtcgtc 12240

gtcccgttgg acccgaggta ccaacgtgat ttgcggaagg actcatgtgt cgggcggttg 12300

cacggcgccc ctgtcctcct gatgtggttg aaacactcgc gtgacgccga ttaccactga 12360

ctctgtggcg tttcactcca catggtcaga cccggtctga taaaaaaggt ctggtcatct 12420

gttccggacg tctggcattt ggactcggtc cgaaagtttt tgaacgtccc cgacaccccc 12480

cacgcccgag ggtgtccgct ggcgcgctgg cacagatcga acgactgcgg gttgagcgcg 12540

gacaacgacg acgattatcg cgggaagtgc ctgtcaccgt cgcacagggc cctgtgtatg 12600

gatccagtga acgactgtga catggcgctc cggtatccag tccgcgtaca cctgctcgta 12660

tgaaaggtcc tctaatgttc acagtcggcg cgcgaccccg tcctcctgtg cccgtcggac 12720

ctccgttggg atttgatgga cgactggttg gccgccgtct tctaggggag caacgtgtca 12780

aatttgtcgc tcctcctcgc gtaaaacgcg atgcacgtcg tctcgcactc ggaattggac 12840

tacgcgctgc cccattgcgg gtcgcaccgc gacctgtact ggcgcgcgtt gtaccttggc 12900

ccgtacatac ggagtttggc cggcaaatag ttggcggatt acctgatgaa cgtagcgcgc 12960

cggcggcact tggggctcat aaagtggtta cggtagaact tgggcgtgac cgatggcggg 13020

ggaccaaaga tgtggccccc taagctccac gggctcccat tgctacctaa ggagaccctg 13080

ctgtatctgc tgtcgcacaa aaggggcgtt ggcgtctggg acgatctcaa cgttgtcgcg 13140

ctcgtccgtc tccgccgcga cgctttcctt tcgaaggcgt ccggttcgtc gaacaggcta 13200

gatccgcgac gccggggcgc cagtctacga tcatcgggta aaggttcgaa ctatcccaga 13260

gaatggtcgt gagcgtggtg ggcgggcgcg gacgacccgc tcctcctcat ggatttgttg 13320

agcgacgacg tcggcgtcgc gctttttttg gacggaggcc gtaaagggtt gttgccctat 13380

ctctcggatc acctgttcta ctcatctacc ttctgcatgc gcgtcctcgt gtccctgcac 13440

ggtccgggcg cgggcgggtg ggcagcagtt tccgtgctgg cagtcgcccc agaccacacc 13500

ctcctgctac tgagccgtct gctgtcgtcg caggacctaa accctccctc accgttgggc 13560

aaacgcgtgg aagcggggtc cgacccctct tacaaaattt tttttttttt cgtactacgt 13620

tttatttttt gagtggttcc ggtaccgtgg ctcgcaacca aaagaacata aggggaatca 13680

tacgccgcgc gccgctacat actccttcca ggaggaggga ggatgctctc acaccactcg 13740

cgccgcggtc accgccgccg cgacccaaga gggaagctac gaggggacct gggcggcaaa 13800

cacggaggcg ccatggacgc cggatggccc ccctctttgt cgtaggcaat gagactcaac 13860

cgtggggata agctgtggtg ggcacacatg gaccacctgt tgttcagttg cctacaccgt 13920

agggacttga tggtcttgct ggtgtcgttg aaagactggt gccagtaagt tttgttactg 13980

atgtcgggcc ccctccgttc gtgtgtctgg tagttagaac tgctggccag cgtgaccccg 14040

ccgctggact tttggtagga cgtatggttg tacggtttac acttgctcaa gtacaaatgg 14100

ttattcaaat tccgcgccca ctaccacagc gcgaacggat gattcctgtt agtccacctc 14160

gactttatgc tcacccacct caagtgcgac gggctcccgt tgatgaggct ctggtactgg 14220

tatctggaat acttgttgcg ctagcacctc gtgatgaact ttcacccgtc tgtcttgccc 14280

caagaccttt cgctgtagcc ccatttcaaa ctgtgggcgt tgaagtctga ccccaaactg 14340

gggcagtgac cagaacagta cggaccccat atatgtttgc ttcggaaggt aggtctgtag 14400

taaaacgacg gtcctacgcc ccacctgaag tgggtgtcgg cggactcgtt gaacaacccg 14460

taggcgttcg ccgttgggaa ggtcctcccg aaatcctagt ggatgctact agacctccca 14520

ccattgtaag ggcgtgacaa cctacacctg cggatggtcc gctcgaactt tctactgtgg 14580

cttgtcccgc ccccaccgcg tccgccgtcg ttgtcgtcac cgtcgccgcg ccttctcttg 14640

aggttgcgcc gtcggcgccg ttacgtcggc cacctcctgt acttgctagt acggtaagcg 14700

ccgctgtgga aacggtgtgc ccgactcctc ttcgcgcgac tccggcttcg tcgccggctt 14760

cgacggcggg ggcgacgcgt tgggctccag ctcttcggag tcttctttgg ccactagttt 14820

ggggactgtc tcctgtcgtt ctttgcgtca atgttggatt attcgttact gtcgtggaag 14880

tgggtcatgg cgtcgaccat ggaacgtatg ttgatgccgc tgggagtctg gccttaggcg 14940

agtacctggg acgaaacgtg aggactgcat tggacgccga gcctcgtcca gatgaccagc 15000

aacggtctgt actacgttct ggggcactgg aaggcgaggt gcgcggtcta gtcgttgaaa 15060

ggccaccacc cgcggctcga caacgggcac gtgaggttct cgaagatgtt gctggtccgg 15120

cagatgaggg ttgagtaggc ggtcaaatgg agagactggg tgcacaagtt agcgaaaggg 15180

ctcttggtct aaaaccgcgc gggcggtcgg gggtggtagt ggtggcagtc acttttgcaa 15240

ggacgagagt gtctagtgcc ctgcgatggc gacgcgttgt cgtagcctcc tcaggtcgct 15300

cactggtaat gactgcggtc tgcggcgtgg acggggatgc aaatgttccg ggacccgtat 15360

cagagcggcg cgcaggatag ctcggcgtga aaaactcgtt cgtacaggta ggaatatagc 15420

gggtcgttat tgtgtccgac cccggacgcg aagggttcgt tctacaaacc gccccggttc 15480

ttcgcgaggc tggttgtggg tcacgcgcac gcgcccgtga tggcgcgcgg gaccccgcgc 15540

gtgtttgcgc cggcgtgacc cgcgtggtgg cagctactgc ggtagctgcg ccaccacctc 15600

ctccgcgcgt tgatgtgcgg gtgcggcggt ggtcacaggt gtcacctgcg ccggtaagtc 15660

tggcaccacg cgcctcgggc cgcgatacga ttttacttct ctgccgcctc cgcgcatcgt 15720

gcagcggtgg cggcggctgg gccgtgacgg cgggttgcgc gccgccgccg ggacgaattg 15780

gcgcgtgcag cgtggccggc tgcccgccgg tacgcccggc gagcttccga ccggcgccca 15840

taacagtgac acggggggtc caggtccgct gctcgccggc ggcgtcgtcg gcgccggtaa 15900

tcacgatact gagtcccagc gtccccgttg cacataaccc acgcgctgag ccaatcgccg 15960

gacgcgcacg ggcacgcgtg ggcggggggc gcgttgatct aacgttcttt tttgatgaat 16020

ctgagcatga caacatacat aggtcgccgc cgccgcgcgt tgcttcgata caggttcgcg 16080

ttttagtttc ttctctacga ggtccagtag cgcggcctct agataccggg gggcttcttc 16140

cttctcgtcc taatgttcgg ggctttcgat ttcgcccagt ttttcttttt ctttctacta 16200

ctactacttg aactgctgct ccaccttgac gacgtgcgat ggcgcgggtc cgctgcccat 16260

gtcacctttc cagctgcgca ttttgcacaa aacgctgggc cgtggtggca tcagaaatgc 16320

gggccactcg cgaggtgggc gtggatgttc gcgcacatac tactccacat gccgctgctc 16380

ctggacgaac tcgtccggtt gctcgcggag cccctcaaac ggatgccttt cgccgtattc 16440

ctgtacgacc gcaacggcga cctgctcccg ttgggttgtg gatcggattt cgggcattgt 16500

gacgtcgtcc acgacgggcg cgaacgtggc aggcttcttt tcgcgccgga tttcgcgctc 16560

agaccactga accgtgggtg gcacgtcgac taccatgggt tcgcggtcgc tgaccttcta 16620

cagaaccttt tttactggca ccttggaccc gacctcgggc tccaggcgca cgccggttag 16680

ttcgtccacc gcggccctga cccgcacgtc tggcacctgc aagtctatgg gtgatggtca 16740

tcgtggtcat aacggtggcg gtgtctcccg tacctctgtg tttgcagggg ccaacggagt 16800

cgccaccgcc tacggcgcca cgtccgccag cgacgccggc gcaggttctg gagatgcctc 16860

cacgtttgcc tgggcaccta caaagcgcaa agtcgggggg ccgcgggcgc ggcaagctcc 16920

ttcatgccgc ggcggtcgcg cgatgacggg cttatacggg atgtaggaag gtaacgcgga 16980

tgggggccga tagcaccgat gtggatggcg gggtcttctg ctcgttgatg ggctgcggct 17040

tggtggtgac cttgggcggc ggcggcagcg gcagcggtcg ggcacgaccg gggctaaagg 17100

cacgcgtccc accgagcgct tcctccgtcc tgggaccacg acggttgtcg cgcgatggtg 17160

gggtcgtagc aaattttcgg ccagaaacac caagaacgtc tataccggga gtggacggcg 17220

gaggcaaagg gccacggccc taaggctcct tcttacgtgg catcctcccc gtaccggccg 17280

gtgccggact gcccgccgta cgcagcacgc gtggtggccg ccgccgcgcg cagcgtggca 17340

gcgtacgcgc cgccatagga cggggaggaa taaggtgact agcggcgccg ctaaccgcgg 17400

cacgggcctt aacgtaggca ccggaacgtc cgcgtctctg tgactaattt ttgttcaacg 17460

tacacctttt tagttttatt tttcagacct gagagtgcga gcgaaccagg acattgataa 17520

aacatcttac cttctgtagt tgaaacgcag agaccggggc gctgtgccga gcgcgggcaa 17580

gtaccctttg accgttctat agccgtggtc gttatactcg ccaccgcgga agtcgacccc 17640

gagcgacacc tcgccgtaat ttttaaagcc aaggtggcaa ttcttgatac cgtcgttccg 17700

gaccttgtcg tcgtgtccgg tctacgactc cctattcaac tttctcgttt taaaggttgt 17760

tttccaccat ctaccggacc ggagaccgta atcgccccac cacctggacc ggttggtccg 17820

tcacgtttta ttctaattgt cattcgaact aggggcggga gggcatctcc tcggaggtgg 17880

ccggcacctc tgtcacagag gtctccccgc accgcttttc gcaggcgcgg ggctgtccct 17940

tctttgagac cactgcgttt atctgctcgg agggagcatg ctcctccgtg atttcgttcc 18000

ggacgggtgg tgggcagggt agcgcgggta ccgatggcct cacgacccgg tcgtgtgtgg 18060

gcattgcgac ctggacggag gggggcggct gtgggtcgtc tttggacacg acggtccggg 18120

ctggcggcaa caacattggg caggatcggc gcgcagggac gcggcgcggc ggtcgccagg 18180

cgctagcaac gccgggcatc ggtcaccgtt gaccgtttcg tgtgacttgt cgtagcaccc 18240

agacccccac gttagggact tcgcggctgc tacgaagact atcgattgca cagcatacac 18300

acagtacata cgcaggtaca gcggcggtct cctcgacgac tcggcggcgc gcgggcgaaa 18360

ggttctaccg atggggaagc tactacggcg tcaccagaat gtacgtgtag agcccggtcc 18420

tgcggagcct catggactcg gggcccgacc acgtcaaacg ggcgcggtgg ctctgcatga 18480

agtcggactt attgttcaaa tctttggggt gccaccgcgg atgcgtgctg cactggtgtc 18540

tggccagggt cgcaaactgc gacgccaagt agggacacct ggcactccta tgacgcatga 18600

gcatgttccg cgccaagtgg gatcgacacc cactattggc acacgacctg taccgaaggt 18660

gcatgaaact gtaggcgccg cacgacctgt ccccgggatg aaaattcggg atgagaccgt 18720

gacggatgtt gcgggaccga gggttcccac ggggtttagg aacgcttacc ctacttcgac 18780

gatgacgaga actttatttg gatcttcttc tcctgctact gttgcttctg cttcatctgc 18840

tcgttcgact cgtcgttttt tgagtgcata aacccgtccg cggaataaga ccatatttat 18900

aatgtttcct cccataagtt tatccacagc ttccagtttg tggatttata cggctatttt 18960

gtaaagttgg acttggagtt tatcctctta gagtcaccat gctttgtctt taattagtac 19020

gtcgaccctc tcaggatttt ttctgatggg gttactttgg tacaatgcca agtatacgtt 19080

ttgggtgttt acttttacct cccgttccgt aagaacattt cgttgtttta cctttcgatc 19140

tttcagttca cctttacgtt aaaaagagtt gatgactccg tcggcgtccg ttaccactat 19200

tgaactgagg atttcaccat aacatgtcac ttctacatct atatctttgg ggtctgtgag 19260

tataaagaat gtacgggtga taattccttc cattgagtgc tcttgattac ccggttgtta 19320

gatacgggtt gtccggatta atgtaacgaa aatccctgtt aaaataacca gattacataa 19380

tgttgtcgtg cccattatac ccacaagacc gcccggttcg tagcgtcaac ttacgacaac 19440

atctaaacgt tctgtctttg tgtctcgaaa gtatggtcga aaacgaacta aggtaaccac 19500

tatcttggtc catgaaaaga tacaccttag tccgacaact gtcgatacta ggtctacaat 19560

cttaataact tttagtacct tgacttctac ttgaaggttt aatgacgaaa ggtgaccctc 19620

cacactaatt atgtctctga gaatggttcc attttggatt ttgtccagtc cttttaccta 19680

ccctttttct acgatgtctt aaaagtctat ttttacttta ttctcaacct ttattaaaac 19740

ggtaccttta gttagattta cggttggaca cctctttaaa ggacatgagg ttgtatcgcg 19800

acataaacgg gctgttcgat ttcatgtcag gaaggttgca tttttaaaga ctattgggtt 19860

tgtggatgct gatgtacttg ttcgctcacc accgagggcc cgatcacctg acgatgtaat 19920

tggaacctcg tgcgaccagg gaactgatat acctgttgca gttgggtaaa ttggtggtgg 19980

cgttacgacc ggacgcgatg gcgagttaca acgacccgtt accagcgata cacgggaagg 20040

tgtaggtcca cggagtcttc aagaaacggt aatttttgga ggaagaggac ggcccgagta 20100

tgtggatgct caccttgaag tccttcctac aattgtacca agacgtctcg agggatcctt 20160

tactggattc ccaactgcct cggtcgtaat tcaaactatc gtaaacggaa atgcggtgga 20220

agaaggggta ccgggtgttg tggcggaggt gcgaactccg gtacgaatct ttgctgtggt 20280

tgctggtcag gaaattgctg atagagaggc ggcggttgta cgagatggga tatgggcggt 20340

tgcgatggtt gcacgggtat aggtagggga gggcgttgac ccgccgaaag gcgccgaccc 20400

ggaagtgcgc ggaattctga ttcctttggg gtagtgaccc gagcccgatg ctgggaataa 20460

tgtggatgag accgagatat gggatggatc taccttggaa aatggagttg gtgtggaaat 20520

tcttccaccg gtaatggaaa ctgagaagac agtcgaccgg accgttactg gcggacgaat 20580

gggggttgct caaactttaa ttcgcgagtc aactgcccct cccaatgttg caacgggtca 20640

cattgtactg gtttctgacc aaggaccatg tttacgatcg attgatattg taaccgatgg 20700

tcccgaagat atagggtctc tcgatgttcc tggcgtacat gaggaagaaa tctttgaagg 20760

tcgggtactc ggcagtccac cacctactat gatttatgtt cctgatggtt gtccacccgt 20820

aggatgtggt tgtgttgttg agacctaaac aaccgatgga acgggggtgg tacgcgcttc 20880

ctgtccggat gggacgattg aaggggatag gcgaatatcc gttctggcgt caactgtcgt 20940

aatgggtctt tttcaaagaa acgctagcgt gggaaaccgc gtagggtaag aggtcattga 21000

aatacaggta cccgcgtgag tgtctggacc cggttttgga agagatgcgg ttgaggcggg 21060

tgcgcgatct gtactgaaaa ctccacctag ggtacctgct cgggtgggaa gaaatacaaa 21120

acaaacttca gaaactgcac caggcacacg tggtcggcgt ggcgccgcag tagctttggc 21180

acatggacgc gtgcgggaag agccggccgt tgcggtgttg tatttcttcg ttcgttgtag 21240

ttgttgtcga cggcggtacc cgaggtcact cgtccttgac tttcggtaac agtttctaga 21300

accaacaccc ggtataaaaa acccgtggat actgttcgcg aaaggtccga aacaaagagg 21360

tgtgttcgag cggacgcggt atcagttatg ccggccagcg ctctgacccc cgcatgtgac 21420

ctaccggaaa cggaccttgg gcgtgagttt ttgtacgatg gagaaactcg ggaaaccgaa 21480

aagactggtc gctgagttcg tccaaatggt caaactcatg ctcagtgagg acgcggcatc 21540

gcggtaacga agaagggggc tggcgacata ttgcgacctt ttcaggtggg tttcgcatgt 21600

ccccgggttg agccggcgga cacctgataa gacgacgtac aaagaggtgc ggaaacggtt 21660

gaccggggtt tgagggtacc tagtgttggg gtggtacttg gaataatggc cccatgggtt 21720

gaggtacgag ttgtcagggg tccatgtcgg gtgggacgca gcgttggtcc ttgtcgagat 21780

gtcgaaggac ctcgcggtga gcgggatgaa ggcgtcggtg tcacgcgtct aatcctcgcg 21840

gtgaagaaaa acagtgaact ttttgtacat ttttattaca tgatctctgt gaaagttatt 21900

tccgtttacg aaaataaaca tgtgagagcc cactaataaa tgggggtggg aacggcagac 21960

gcggcaaatt tttagtttcc ccaagacggc gcgtagcgat acgcggtgac cgtccctgtg 22020

caacgctatg accacaaatc acgaggtgaa tttgagtccg tgttggtagg cgccgtcgag 22080

ccacttcaaa agtgaggtgt ccgacgcgtg gtagtggttg cgcaaatcgt ccagcccgcg 22140

gctatagaac ttcagcgtca accccggagg cgggacgcgc gcgctcaacg ctatgtgtcc 22200

caacgtcgtg accttgtgat agtcgcggcc caccacgtgc gaccggtcgt gcgagaacag 22260

cctctagtct aggcgcaggt ccaggaggcg caacgagtcc cgcttgcctc agttgaaacc 22320

atcgacggaa gggtttttcc cgcgcacggg tccgaaactc aacgtgagcg tggcatcacc 22380

gtagttttcc actggcacgg gccagacccg caatcctatg tcgcggacgt attttcggaa 22440

ctagacgaat tttcggtgga ctcggaaacg cggaagtctc ttcttgtacg gcgttctgaa 22500

cggccttttg actaaccggc ctgtccggcg cagcacgtgc gtcgtggaac gcagccacaa 22560

cctctagacg tggtgtaaag ccggggtggc caagaagtgc tagaaccgga acgatctgac 22620

gaggaagtcg cgcgcgacgg gcaaaagcga gcagtgtagg taaagttagt gcacgaggaa 22680

taaatagtat tacgaaggca catctgtgaa ttcgagcgga agctagagtc gcgtcgccac 22740

gtcggtgttg cgcgtcgggc acccgagcac tacgaacatc cagtggagac gtttgctgac 22800

gtccatgcgg acgtccttag cggggtagta gcagtgtttc cagaacaacg accacttcca 22860

gtcgacgttg ggcgccacga ggagcaagtc ggtccagaac gtatgccggc ggtctcgaag 22920

gtgaaccagt ccgtcatcaa acttcaagcg gaaatctagc aataggtgca ccatgaacag 22980

gtagtcgcgc gcgcgtcgga ggtacgggaa gagggtgcgt ctgtgctagc cgtgtgagtc 23040

gcccaagtag tggcattaaa gtgaaaggcg aagcgacccg agaaggagaa ggagaacgca 23100

ggcgtatggt gcgcggtgac ccagcagaag taagtcggcg gcgtgacacg cgaatggagg 23160

aaacggtacg aactaatcgt ggccacccaa cgactttggg tggtaaacat cgcggtgtag 23220

aagagaaaga aggagcgaca ggtgctaatg gagaccacta ccgcccgcga gcccgaaccc 23280

tcttcccgcg aagaaaaaga agaacccgcg ttaccggttt aggcggcggc tccagctacc 23340

ggcgcccgac ccacacgcgc cgtggtcgcg cagaacacta ctcagaagga gcaggagcct 23400

gagctatgcg gcggagtagg cgaaaaaacc cccgcgggcc cctccgccgc cgctgcccct 23460

gcccctgctg tgcaggaggt accaaccccc tgcagcgcgg cgtggcgcag gcgcgagccc 23520

ccaccaaagc gcgacgagga gaagggctga ccggtaaagg aagaggatat ccgtcttttt 23580

ctagtacctc agtcagctct tcttcctgtc ggattggcgg gggagactca agcggtggtg 23640

gcggaggtgg ctacggcggt tgcgcggatg gtggaagggg cagctccgtg ggggcgaact 23700

cctcctcctt cactaatagc tcgtcctggg tccaaaacat tcgcttctgc tgctcctggc 23760

gagtcatggt tgtctcctat ttttcgttct ggtcctgttg cgtctccgtt tgctccttgt 23820

tcagcccgcc cccctgcttt ccgtaccgct gatggatcta caccctctgc tgcacgacaa 23880

cttcgtagac gtcgcggtca cgcggtaata gacgctgcgc aacgttctcg cgtcgctaca 23940

cggggagcgg tatcgcctac agtcggaacg gatgcttgcg gtggataaga gtggcgcgca 24000

tggggggttt gcggttcttt tgccgtgtac gctcgggttg ggcgcggagt tgaagatggg 24060

gcataaacgg cacggtctcc acgaacggtg gatagtgtag aaaaaggttt tgacgttcta 24120

tggggatagg acggcacggt tggcgtcggc tcgcctgttc gtcgaccgga acgccgtccc 24180

gcgacagtat ggactatagc ggagcgagtt gcttcacggt ttttagaaac tcccagaacc 24240

tgcgctgctc ttcgcgcgcc gtttgcgaga cgttgtcctt ttgtcgcttt tactttcagt 24300

gagacctcac aaccaccttg agctcccact gttgcgcgcg gatcggcatg attttgcgtc 24360

gtagctccag tgggtgaaac ggatgggccg tgaattggat ggggggttcc agtactcgtg 24420

tcagtactca ctcgactagc acgcggcacg cgtcggggac ctctccctac gtttaaacgt 24480

tcttgtttgt ctcctcccgg atgggcgtca accgctgctc gtcgatcgcg cgaccgaagt 24540

ttgcgcgctc ggacggctga acctcctcgc tgcgtttgat tactaccggc gtcacgagca 24600

atggcacctc gaactcacgt acgtcgccaa gaaacgactg ggcctctacg tcgcgttcga 24660

tctcctttgt aacgtgatgt ggaaagctgt cccgatgcat gcggtccgga cgttctagag 24720

gttgcacctc gagacgttgg accagaggat ggaaccttaa aacgtgcttt tggcggaacc 24780

cgttttgcac gaagtaaggt gcgagttccc gctccgcgcg gcgctgatgc aggcgctgac 24840

gcaaatgaat aaagatacga tgtggaccgt ctgccggtac ccgcaaaccg tcgtcacgaa 24900

cctcctcacg ttggagttcc tcgacgtctt tgacgatttc gttttgaact tcctggatac 24960

ctgccggaag ttgctcgcga ggcaccggcg cgtggaccgc ctgtagtaaa aggggcttgc 25020

ggacgaattt tgggacgttg tcccagacgg tctgaagtgg tcagtttcgt acaacgtctt 25080

gaaatccttg aaataggatc tcgcgagtcc ttagaacggg cggtggacga cacgtgaagg 25140

atcgctgaaa cacgggtaat tcatggcgct tacgggaggc ggcgaaaccc cggtgacgat 25200

ggaagacgtc gatcggttga tggaacggat ggtgagactg tattaccttc tgcactcgcc 25260

actgccagat gacctcacag tgacagcgac gttggatacg tggggcgtgg cgagggacca 25320

aacgttaagc gtcgacgaat tgctttcagt ttaatagcca tggaaactcg acgtcccagg 25380

gagcggactg cttttcaggc gccgaggccc caactttgag tgaggccccg acacctgcag 25440

ccgaatggaa gcgtttaaac atggactcct gatggtgcgg gtgctctaat ccaagatgct 25500

tctggttagg gcgggcggat tacgcctcga atggcggacg cagtaatggg tcccggtgta 25560

agaaccggtt aacgttcggt agttgtttcg ggcggttctc aaagacgatg ctttccctgc 25620

cccccaaatg aacctggggg tcaggccgct cctcgagttg ggttaggggg gcggcggcgt 25680

cgggatagtc gtcgtcggcg cccgggaacg aagggtccta ccgtgggttt ttcttcgacg 25740

tcgacggcgg cggtgggtgc ctgctcctcc ttatgaccct gtcagtccgt ctcctccaaa 25800

acctgctcct cctcctcctg tactaccttc tgaccctctc ggatctgctc cttcgaaggc 25860

tccagcttct ccacagtctg ctttgtggca gtgggagcca gcgtaagggg agcggccgcg 25920

gggtctttag ccgttggcca aggtcgtacc gatgttggag gcgaggagtc cgcggcggcc 25980

gtgacgggca agcggctggg ttggcatcta ccctgtggtg accttggtcc cggccattca 26040

ggttcgtcgg cggcggcaat cgggttctcg ttgttgtcgc ggttccgatg gcgagtaccg 26100

cgcccgtgtt cttgcggtat caacgaacga acgttctgac acccccgttg tagaggaagc 26160

gggcggcgaa agaagagatg gtagtgccgc accggaaggg ggcattgtag gacgtaatga 26220

tggcagtaga gatgtcgggt atgacgtggc cgccgtcgcc gtcgttgtcg tcgccggtgt 26280

gtcttcgttt ccgctggcct atcgttctga gactgtttcg ggttctttag gtgtcgccgc 26340

cgtcgtcgtc ctcctcctcg cgacgcagac cgcgggttgc ttgggcatag ctgggcgctc 26400

gaatctttgt cctaaaaagg gtgagacata cgatataaag ttgtctcgtc cccggttctt 26460

gttctcgact tttatttttt gtccagagac gctagggagt gggcgtcgac ggacatagtg 26520

ttttcgcttc tagtcgaagc cgcgtgcgac cttctgcgcc tccgagagaa gtcatttatg 26580

acgcgcgact gagaattcct gatcaaagcg cgggaaagag tttaaattcg cgcttttgat 26640

gcagtagagg tcgccggtgt gggccgcggt cgtggacaac agtcgcggta atactcgttc 26700

ctttaagggt gcgggatgta cacctcaatg gtcggtgttt accctgaacg ccgacctcga 26760

cgggttctga tgagttgggc ttatttgatg tactcgcgcc ctggggtgta ctatagggcc 26820

cagttgcctt atgcgcgggt ggctttggct taagaggacc ttgtccgccg ataatggtgg 26880

tgtggagcat tattggaatt aggggcatca accgggcgac gggaccacat ggtcctttca 26940

gggcgagggt ggtgacacca tgaagggtct ctgcgggtcc ggcttcaagt ctactgattg 27000

agtccccgcg tcgaacgccc gccgaaagca gtgtcccacg ccagcgggcc cgtcccatat 27060

tgagtggact gttagtctcc cgctccataa gtcgagttgc tgctcagcca ctcgaggagc 27120

gaaccagagg caggcctgcc ctgtaaagtc tagccgccgc ggccggcgag aagtaagtgc 27180

ggagcagtcc gttaggattg agacgtctgg agcaggagac tcggcgcgag acctccgtaa 27240

ccttgagacg ttaaataact cctcaaacac ggtagccaga tgaaattggg gaagagccct 27300

ggagggccgg tgataggcct agttaaataa ggattgaaac tgcgccattt cctgagccgc 27360

ctgccgatgc tgacttacaa ttcacctctc cgtctcgttg acgcggactt tgtggaccag 27420

gtgacagcgg cggtgttcac gaaacgggcg ctgaggccac tcaaaacgat gaaacttaac 27480

gggctcctag tatagctccc gggccgcgtg ccgcaggccg aatggcgggt ccctctcgaa 27540

cgggcatcgg actaagccct caaatgggtc gcgggggacg atcaactcgc cctgtcccct 27600

gggacacaag agtgacacta aacgttgaca ggattgggac ctaatgtagt tctagaaaca 27660

acggtagaga cacgactcat attatttatg tctttaattt tatatgaccc cgaggatagc 27720

ggtaggacat ttgcggtggc agaagtgggc gggttcgttt ggttccgctt ggaatggacc 27780

atgaaaattg tagagaggga gacactaaat gttgtcaaag ttgggtctgc ctcactcaga 27840

tgctctcttg gagaggctcg agtcgatgag gtagtctttt ttgtggtggg aggaatggac 27900

ggcccttgca tgctcacgca gtggccggcg acgtggtgtg gatggcggac tggcatttgg 27960

tctgaaaaag gcctgtctgg agttattgag acaaatggtc ttgtcctcca ctcgaatctt 28020

ttgggaatcc cataatccgg tttccgcgtc gatgacaccc caaatacttg ttaagttcgt 28080

tgagatgccc gataagatta agtccaaaga gatcttagcc ccaaccccaa taagagacag 28140

aacactaaga gaaataagaa tatgattgcg aagagacgga ttccgagcgg cggacgacac 28200

acgtgtaaac gtaaataaca gtcgaaaaat ttgcgacccc agcggtgggt tctactaatc 28260

catgtattag gatccaaatg agtgggaacg cagtcgggtg ccatggtggg ttttccacct 28320

aaaattcctc ggtcggacat tacaatgtaa gcgtcgactt cgattactca cgtggtgaga 28380

atattttacg tggtgtcttg tacttttcga cgaataagcg gtgtttttgt tttaaccgtt 28440

catacgacaa atacgataaa ccgtcggtcc actgtgatgt ctcatattac aatgtcaaaa 28500

ggtcccattt tcagtatttt gaaaatacat atgaaaaggt aaaatacttt acacgctgta 28560

atggtacatg tactcgtttg tcatattcaa caccgggggt gttttaacac accttttgtg 28620

accgtgaaag acgacgtgac gatacgatta atgtcacgag cgaaaccaga catgggatga 28680

gatataattt atgttttcgt ctgcgtcgaa ataactcctt ttcttttacg gaattaaatg 28740

attcaatgtt tcgattacag tggtgattga cgaaatgagc gacgaacgtt ttgtttaagt 28800

ttttcaatcg taatattaat cttatcctaa atttgggggg ccagtaaagg acgagttatg 28860

gtaaggggac ttgttaactg agatacaccc tatacgaggt cgcgatgttg gaacttcagt 28920

ccgaaggacc tacagtcgta gactgaaacc ggtcgtggac agggcgccta aacaaggtca 28980

ggttgatgtc gctgggtggg attgtctcta ctggttgtgt tggttgcgcc ggcggcgatg 29040

gcctgaatgt agatggtgtt tatgtggggt tcaaagacgg aaacagttat tgaccctatt 29100

gaacccgtac accaccaaga ggtatcgcga atacaaacat acggaataat aatacaccga 29160

gtagacgacg gatttcgcgt ttgcgcgggc tggtgggtag atatcagggt agtaacacga 29220

tgtgggtttg ttactacctt aggtatctaa cctgcctgac tttgtgtaca agaaaagaga 29280

atgtcatact aatttactct gtactaagga gctcaaaaat ataatgactg ggaacaacgc 29340

gaaaaaacac gcacgaggtg taaccgacgc caaagagtgt agcttcatct gacgtaaggt 29400

cggaagtgtc agataaacga aatgcctaaa cagtgggagt gcgagtagac gtcggagtag 29460

tgacaccagt agcggaaata ggtcacgtaa ctgacccaga cacacgcgaa acgtatagag 29520

tctgtggtag gggtcatgtc cctgtcctga tatcgactcg aagaatctta agaaattaat 29580

actttaaatg acactgaaaa gacgactaat aaacgtggga tagacgcaaa acaaggggct 29640

ggaggttcgg agtttctgta tatagtacgt ctaagtgagc atatacctta taaggttcaa 29700

cgatgttact tttttcgcta gaaaggcttc ggaccaatat acgttagtag agacaatacc 29760

acaagacgtc atggtagaat cgggatcgat atatagggat ggaactgtaa ccgaccttgc 29820

gttatctacg gtacttggtg ggttgaaagg ggcgcgggcg atacgaaggt gacgttgttc 29880

aacaacggcc gccgaaacag ggtcggttag tcggagcggg tggaagaggg tgggggtgac 29940

tttagtcgat gaaattagat tgtcctcctc tactgactgt gggatctaga tctttacctg 30000

ccttaataat gtctcgtcgc ggacgatctt tctgcgtccc gtcgccggct cgttgtcgcg 30060

tacttagttc tcgaggttct gtaccaattg aacgtggtca cgttttcccc atagaaaaca 30120

gagcatttcg tccggtttca gtggatgctg tcattatggt ggcctgtggc ggaatcgatg 30180

ttcaacggtt ggttcgcagt ctttaaccac cagtaccacc ctcttttcgg gtaatggtat 30240

tgagtcgtga gccatctttg gcttccgacg taagtgagtg gaacagttcc tggactccta 30300

gagacgtggg aataattctg ggacacgcca gagtttctag aataagggaa attgattatt 30360

tttttttatt atttcgtagt gaatgaattt tagtcaatcg tttaaagaca ggtcaaataa 30420

gtcgtcgtgg aggaacggga ggagggtcga gaccataacg tcgaaggagg accgacgttt 30480

gaaagaggtg ttagatttac cttacagtca aaggaggaca aggacaggta ggcgtgggtg 30540

atagaagtac aacaacgtct acttcgcgcg ttctggcaga cttctatgga agttggggca 30600

cataggtata ctgtgccttt ggccaggagg ttgacacgga aaagaatgag gagggaaaca 30660

tagggggtta cccaaagttc tctcaggggg accccatgag agaaacgcgg ataggcttgg 30720

agatcaatgg aggttaccgt acgaacgcga gttttacccg ttgccggaga gagacctgct 30780

ccggccgttg gaatggaggg ttttacattg gtgacactcg ggtggagagt ttttttggtt 30840

cagtttgtat ttggaccttt atagacgtgg ggagtgtcaa tggagtcttc gggattgaca 30900

ccgacggcgg cgtggagatt accagcgccc gttgtgtgag tggtacgtta gtgtccgggg 30960

cgattggcac gtgctgaggt ttgaatcgta acggtgggtt cctggggagt gtcacagtct 31020

tcctttcgat cgggacgttt gtagtccggg ggagtggtgg tggctatcgt catgggaatg 31080

atagtgacgg agtgggggaa attgatgacg gtgaccatcg aacccgtaac tgaactttct 31140

cgggtaaata tgtgttttac cttttgatcc tgatttcatg ccccgaggaa acgtacattg 31200

tctgctggat ttgtgaaact ggcatcgttg accaggtcca cactgataat tattatgaag 31260

gaacgtttga tttcaatgac ctcggaaccc aaaactaagt gttccgttat acgttgaatt 31320

acatcgtcct cctgattcct aactaagagt tttgtctgcg gaatatgaac tacaatcaat 31380

aggcaaacta cgagttttgg ttgatttaga ttctgatcct gtcccgggag aaaaatattt 31440

gagtcgggtg ttgaacctat aattgatgtt gtttccggaa atgaacaaat gtcgaagttt 31500

gttaaggttt ttcgaactcc aattggattc gtgacggttc cccaactaca aactgcgatg 31560

tcggtatcgg taattacgtc ctctacccga acttaaacca agtggattac gtggtttgtg 31620

tttaggggag ttttgttttt aaccggtacc ggatcttaaa ctaagtttgt tccgatacca 31680

aggatttgat ccttgaccgg aatcaaaact gtcgtgtcca cggtaatgtc atcctttgtt 31740

tttattacta ttcgattgaa acacctggtg tggtcgaggt agaggattga catctgattt 31800

acgtctcttt ctacgatttg agtgaaacca gaattgtttt acaccgtcag tttatgaacg 31860

atgtcaaagt caaaaccgac aatttccgtc aaaccgaggt tatagacctt gtcaagtttc 31920

acgagtagaa taatattcta aactgctttt acctcacgat gatttgttaa ggaaggacct 31980

gggtcttata accttgaaat ctttacctct agaatgactt ccgtgtcgga tatgtttgcg 32040

acaacctaaa tacggattgg atagtcgaat aggttttaga gtgccatttt gacggttttc 32100

attgtaacag tcagttcaaa tgaatttgcc tctgttttga tttggacatt gtgattggta 32160

atgtgatttg ccatgtgtcc tttgtcctct gtgttgaggt tcacgtatga gatacagtaa 32220

aagtaccctg accagaccgg tgttgatgta attactttat aaacggtgta ggagaatgtg 32280

aaaaagtatg taacgggttc ttatttctta gcaaacacaa tacaaagttg cacaaataaa 32340

aagttaacgt cttttaaagt tcagtaaaaa gtaagtcatc atatcggggt ggtggtgtat 32400

cgaatatgtc tagtggcatg gaattagttt gagtgtcttg ggatcataag ttggacggtg 32460

gagggagggt tgtgtgtctc atgtgtcagg aaagaggggc cgaccggaat ttttcgtagt 32520

atagtaccca ttgtctgtat aagaatccac aatataaggt gtgccaaagg acagctcggt 32580

ttgcgagtag tcactataat tatttgaggg gcccgtcgag tgaattcaag tacagcgaca 32640

ggtcgacgac tcggtgtccg acgacaggtt gaacgccaac gaattgcccg ccgcttcctc 32700

ttcaggtgcg gatgtacccc catctcagta ttagcacgta gtcctatccc gccaccacga 32760

cgtcgtcgcg cgcttatttg acgacggcgg cggcgaggca ggacgtcctt atgttgtacc 32820

gtcaccagag gagtcgctac taagcgtggc gggcgtcgta ttccgcggaa caggaggccc 32880

gtgtcgtcgc gtgggactag agtgaattta gtcgtgtcat tgacgtcgtg tcgtggtgtt 32940

ataacaagtt ttagggtgtc acgttccgcg acataggttt cgagtaccgc ccctggtgtc 33000

ttgggtgcac cggtagtatg gtgttcgcgt ccatctaatt caccgctggg gagtatttgt 33060

gcgacctgta tttgtaatgg agaaaaccgt acaacattaa gtggtggagg gccatggtat 33120

atttggagac taatttgtac cgcggtaggt ggtggtagga tttggtcgac cggttttgga 33180

cgggcggccg atatgtgacg tcccttggcc ctgaccttgt tactgtcacc tctcgggtcc 33240

tgagcattgg tacctagtag tacgagcagt actatagtta caaccgtgtt gtgtccgtgt 33300

gcacgtatgt gaaggagtcc taatgttcga ggagggcgca atcttggtat agggtccctt 33360

gttgggtaag gacttagtcg catttagggt gtgacgtccc ttctggagcg tgcattgagt 33420

gcaacacgta acagtttcac aatgtaagcc cgtcgtcgcc tactaggagg tcataccatc 33480

gcgcccaaag acagagtttt cctccatctg ctagggatga catgcctcac gcggctctgt 33540

tggctctagc acaaccagca tcacagtacg gtttaccttg cggcctgcat cagtataaag 33600

gacttcgttt tggtccacgc ccgcactgtt tgtctagacg cagaggccag agcggcgaat 33660

ctagcgagac acatcatcaa catcatatag gtgagagagt ttcgtaggtc cgcgggggac 33720

cgaagcccaa gatacatttg aggaagtacg cggcgacggg actattgtag gtggtggcgt 33780

cttattcggt gtgggtcggt tggatgtgta agcaagacgc tcagtgtgtg ccctcctcgc 33840

ccttctcgac cttcttggta caaaaaaaaa aataaggttt tctaataggt tttggagttt 33900

tacttctaga taattcactt gcgcgagggg aggccaccgc accagtttga gatgtcggtt 33960

tcttgtctat taccgtaaac attctacaac gtgttaccga aggttttccg tttgccggga 34020

gtgcaggttc acctgcattt ccgatttggg aagtcccact tagaggagat atttgtaagg 34080

tcgtggaagt tggtacgggt ttattaagag tagagcggtg gaagagttat atagagattc 34140

gtttagggct tataattcag gccggtaaca tttttagacg aggtctcgcg ggaggtggaa 34200

gtcggagttc gtcgcttagt actaacgttt ttaagtccaa ggagtgtctg gacatattct 34260

aagttttcgc cttgtaattg tttttatggc gctagggcat ccagggaagc gtcccggtcg 34320

acttgtatta gcacgtccag acgtgcctgg tcgcgccggt gaaggggcgg tccttggtac 34380

tgttttcttg ggtgtgacta atactgtgcg tatgagcctc gatacgattg gtcgcatcgg 34440

ggctacattc gaacaacgta cccgccgcta tattttacgt tccacgacga gttttttagt 34500

ccgtttcgga gcgcgttttt tctttcgtgt agcatcagta cgagtacgtc tatttccgtc 34560

cattcgaggc cttggtggtg tctttttctg tggtaaaaag agagtttgta cagacgccca 34620

aagacgtatt tgtgttttat tttattgttt ttttgtaaat ttgtaatctt cggacagaat 34680

gttgtccttt ttgttgggaa tattcgtatt ctgcctgatg ccggtacggc cgcactggca 34740

tttttttgac cagtggcact aatttttcgt ggtggctgtc gaggagccag tacaggcctc 34800

agtattacat tctgagccat ttgtgtagtc caactaagtg tagccagtca cgatttttcg 34860

ctggctttat cgggccccct tatgtatggg cgtccgcatc tctgttgtaa tgtcgggggt 34920

atcctccata ttgttttaat tatcctctct ttttgtgtat ttgtggactt tttgggagga 34980

cggatccgtt ttatcgtggg agggcgaggt cttgttgtat gtcgcgaagg tgtcgccgtc 35040

ggtattgtca gtcggaatgg tcattttttc ttttggataa tttttttgtg gtgagctgtg 35100

ccgtggtcga gttagtcagt gtcacatttt ttcccggttc acgtctcgct catatatatc 35160

ctgatttttt actgcattgc caatttcagg tgttttttgt gggtcttttg gcgtgcgctt 35220

ggatgcgggt ctttgctttc ggttttttgg gtgttgaagg agtttagcag tgaaggcaaa 35280

agggtgcaat gcagtgaagg gtaaaattct tttgatgtta agggttgtgt atgttcaatg 35340

aggcgggatt ttggatgcag tgggcggggc aagggtgcgg ggcgcggtgc agtgtttgag 35400

gtgggggagt aatagtataa ccgaagttag gttttattcc atataataac tactacaatt 35460

aattcttaag cctagacgct gcgctccgac ctaccggaag gggtaatact aagaagagcg 35520

aaggccgccg tagccctacg ggcgcaacgt ccggtacgac aggtccgtcc atctactgct 35580

ggtagtccct gtcgaagttc cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga 35640

ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg cgagttcagt 35700

ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac cttcgaggga 35760

gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga aagagggaag 35820

cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc acatccagca 35880

agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga cgcggaatag 35940

gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg 36000

gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca agaacttcac 36060

caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag acgacttcgg 36120

tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt ggcgaccatc 36180

gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta gagttcttct 36240

aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg caattcccta 36300

aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttag ttagatttca 36360

tatatactca tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt 36420

cgctagacag ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc 36480

tatgccctcc cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt 36540

ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca 36600

ggacgttgaa ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca 36660

tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt 36720

gcgagcagca aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt 36780

actagggggt acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct 36840

tcattcaacc ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga 36900

cagtacggta ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact 36960

cttatcacat acgccgctgg ctcaacgaga acgggccgca gttgtgccct attatggcgc 37020

ggtgtatcgt cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag 37080

agttcctaga atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact 37140

agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta 37200

cggcgttttt tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa 37260

gttataataa cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca 37320

taaatctttt tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg 37380

cagattcttt ggtaataata gtactgtaat tggatatttt tatccgcata gtgctccggg 37440

aaagcagaag ttcttaacct aggcttaaga atta 37474



8
10
DNA
Artificial Sequence

adenovirus serotype 34


8

gtgagtccta 10



9
11
DNA
Artificial Sequence

adenovirus serotype 34


9

ccgcactttc t 11



10
11
DNA
Artificial Sequence

adenovirus serotype 34


10

attgacattg g 11



11
12
DNA
Artificial Sequence

adenovirus serotype 34


11

cagtctggag ga 12



12
6
DNA
Artificial Sequence

adenovirus serotype 34


12

ggagga 6



13
34796
DNA
adenovirus serotype 35

13

catcatcaat aatatacctt atagatggaa tggtgccaat atgtaaatga ggtgatttta 60

aaaagtgtgg gccgtgtggt gattggctgt ggggttaacg gttaaaaggg gcggcgcggc 120

cgtgggaaaa tgacgtttta tgggggtgga gtttttttgc aagttgtcgc gggaaatgtt 180

acgcataaaa aggcttcttt tctcacggaa ctacttagtt ttcccacggt atttaacagg 240

aaatgaggta gttttgaccg gatgcaagtg aaaattgctg attttcgcgc gaaaactgaa 300

tgaggaagtg tttttctgaa taatgtggta tttatggcag ggtggagtat ttgttcaggg 360

ccaggtagac tttgacccat tacgtggagg tttcgattac cgtgtttttt acctgaattt 420

ccgcgtaccg tgtcaaagtc ttctgttttt acgtaggtgt cagctgatcg ctagggtatt 480

tatacctcag ggtttgtgtc aagaggccac tcttgagtgc cagcgagaag agttttctcc 540

tctgcgccgg cagtttaata ataaaaaaat gagagatttg cgatttctgc ctcaggaaat 600

aatctctgct gagactggaa atgaaatatt ggagcttgtg gtgcacgccc tgatgggaga 660

cgatccggag ccacctgtgc agctttttga gcctcctacg cttcaggaac tgtatgattt 720

agaggtagag ggatcggagg attctaatga ggaagctgtg aatggctttt ttaccgattc 780

tatgctttta gctgctaatg aaggattaga attagatccg cctttggaca ctttcaatac 840

tccaggggtg attgtggaaa gcggtacagg tgtaagaaaa ttacctgatt tgagttccgt 900

ggactgtgat ttgcactgct atgaagacgg gtttcctccg agtgatgagg aggaccatga 960

aaaggagcag tccatgcaga ctgcagcggg tgagggagtg aaggctgcca atgttggttt 1020

tcagttggat tgcccggagc ttctggacat ggctgtaagt cttgtgaatt tcacaggaaa 1080

aatactggag taaaggaact gttatgttcg cttttgttat atgaaaaccc actgccactt 1140

tatttacagt aaagtgtgtt taagttaaaa tttaaaggaa tatgctgttt ttcacatgta 1200

tattgagtgt gagttttgtg cttcttatta taagtcctgt gtctgatgct gatgaatcac 1260

catctcctga ttctactacc tcacctcctg atattcaagc acctgttcct gtggacgtgc 1320

gcaagcccat tcctgtgaag cttaagcctg ggaaacgtcc agcagtggag aaacttgagg 1380

acttgttaca gggtggggac ggacctttgg acttgagtac acggaaacgt ccaagacaat 1440

aagtgttcca tatccgtgtt tacttaaggt gacgtcaata tttgtgtgag agtgcaatgt 1500

aataaaaata tgttaactgt tcactggttt ttattgcttt ttgggcgggg actcaggtat 1560

ataagtagaa gcagacctgt gtggttagct cataggagct ggctttcatc catggaggtt 1620

tgggccattt tggaagacct taggaagact aggcaactgt tagagagcgc ttcggacgga 1680

gtctccggtt tttggagatt ctggttcgct agtgaattag ctagggtagt ttttaggata 1740

aaacaggact ataaacaaga atttgaaaag ttgttggtag attgcccagg actttttgaa 1800

gctcttaatt tgggccatca ggttcacttt aaagaaaaag ttttatcagt tttagacttt 1860

tcaaccccag gtagaactgc tgctgctgtg gcttttctta cttttatatt agataaatgg 1920

atcccgcaga ctcatttcag caggggatac gttttggatt tcatagccac agcattgtgg 1980

agaacatgga aggttcgcaa gatgaggaca atcttaggtt actggccagt gcagcctttg 2040

ggtgtagcgg gaatcctgag gcatccaccg gtcatgccag cggttctgga ggaggaacag 2100

caagaggaca acccgagagc cggcctggac cctccagtgg aggaggcgga gtagctgact 2160

tgtctcctga actgcaacgg gtgcttactg gatctacgtc cactggacgg gataggggcg 2220

ttaagaggga gagggcatcc agtggtactg atgctagatc tgagttggct ttaagtttaa 2280

tgagtcgcag acgtcctgaa accatttggt ggcatgaggt tcagaaagag ggaagggatg 2340

aagtttctgt attgcaggag aaatattcac tggaacaggt gaaaacatgt tggttggagc 2400

cagaggatga ttgggaggtg gccattaaaa attatgccaa gatagctttg aggcctgata 2460

aacagtataa gatcagtaga cggattaata tccggaatgc ttgttacata tctggaaatg 2520

gggctgaggt ggtaatagat actcaagaca agacagttat tagatgctgc atgatggata 2580

tgtggcctgg agtagtcggt atggaagcag tcacttttgt aaatgttaag tttaggggag 2640

atggttataa tggaatagtg tttatggcca ataccaaact tatattgcat ggttgtagct 2700

tttttggttt caacaatacc tgtgtagatg cctggggaca ggttagtgta cgggggtgta 2760

gtttctatgc gtgttggatt gccacagctg gcagaaccaa gagtcaattg tctctgaaga 2820

aatgcatatt ccaaagatgt aacctgggca ttctgaatga aggcgaagca agggtccgtc 2880

actgcgcttc tacagatact ggatgtttta ttttaattaa gggaaatgcc agcgtaaagc 2940

ataacatgat ttgtggtgct tccgatgaga ggccttatca aatgctcact tgtgctggtg 3000

ggcattgtaa tatgctggct actgtgcata ttgtttccca tcaacgcaaa aaatggcctg 3060

tttttgatca caatgtgttg accaagtgca ccatgcatgc aggtgggcgt agaggaatgt 3120

ttatgcctta ccagtgtaac atgaatcatg tgaaagtgtt gttggaacca gatgcctttt 3180

ccagaatgag cctaacagga atctttgaca tgaacacgca aatctggaag atcctgaggt 3240

atgatgatac gagatcgagg gtgcgcgcat gcgaatgcgg aggcaagcat gccaggttcc 3300

agccggtgtg tgtagatgtg accgaagatc tcagaccgga tcatttggtt attgcccgca 3360

ctggagcaga gttcggatcc agtggagaag aaactgacta aggtgagtat tgggaaaact 3420

ttggggtggg attttcagat ggacagattg agtaaaaatt tgttttttct gtcttgcagc 3480

tgacatgagt ggaaatgctt cttttaaggg gggagtcttc agcccttatc tgacagggcg 3540

tctcccatcc tgggcaggag ttcgtcagaa tgttatggga tctactgtgg atggaagacc 3600

cgttcaaccc gccaattctt caacgctgac ctatgctact ttaagttctt cacctttgga 3660

cgcagctgca gccgctgccg ccgcctctgt cgccgctaac actgtgcttg gaatgggtta 3720

ctatggaagc atcgtggcta attccacttc ctctaataac ccttctacac tgactcagga 3780

caagttactt gtccttttgg cccagctgga ggctttgacc caacgtctgg gtgaactttc 3840

tcagcaggtg gccgagttgc gagtacaaac tgagtctgct gtcggcacgg caaagtctaa 3900

ataaaaaaaa ttccagaatc aatgaataaa taaacgagct tgttgttgat ttaaaatcaa 3960

gtgtttttat ttcatttttc gcgcacggta tgccctggac caccgatctc gatcattgag 4020

aactcggtgg attttttcca gaatcctata gaggtgggat tgaatgttta gatacatggg 4080

cattaggccg tctttggggt ggagatagct ccattgaagg gattcatgct ccggggtagt 4140

gttgtaaatc acccagtcat aacaaggtcg cagtgcatgg tgttgcacaa tatcttttag 4200

aagtaggctg attgccacag ataagccctt ggtgtaggtg tttacaaacc ggttgagctg 4260

ggaggggtgc attcgaggtg aaattatgtg cattttggat tggattttta agttggcaat 4320

attgccgcca agatcccgtc ttgggttcat gttatgaagg actaccaaga cggtgtatcc 4380

ggtacattta ggaaatttat cgtgcagctt ggatggaaaa gcgtggaaaa atttggagac 4440

acccttgtgt cctccgagat tttccatgca ctcatccatg ataatagcaa tggggccgtg 4500

ggcagcggcg cgggcaaaca cgttccgtgg gtctgacaca tcatagttat gttcctgagt 4560

taaatcatca taagccattt taatgaattt ggggcggagc gtaccagatt ggggtatgaa 4620

tgttccttcg ggccccggag catagttccc ctcacagatt tgcatttccc aagctttcag 4680

ttctgagggt ggaatcatgt ccacctgggg ggctatgaag aacaccgttt cgggggcggg 4740

ggtgattagt tgggatgata gcaagtttct gagcaattga gatttgccac atccggtggg 4800

gccataaata attccgatta caggttgcag gtggtagttt agggaacggc aactgccgtc 4860

ttctcgaagc aagggggcca cctcgttcat catttccctt acatgcatat tttcccgcac 4920

caaatccatt aggaggcgct ctcctcctag tgatagaagt tcttgtagtg aggaaaagtt 4980

tttcagcggt tttagaccgt cagccatggg cattttggaa agagtttgct gcaaaagttc 5040

tagtctgttc cacagttcag tgatgtgttc tatggcatct cgatccagca gacctcctcg 5100

tttcgcgggt ttggacggct cctggagtag ggtatgagac gatgggcgtc cagcgctgcc 5160

agggttcggt ccttccaggg tctcagtgtt cgagtcaggg ttgtttccgt cacagtgaag 5220

gggtgtgcgc ctgcttgggc gcttgccagg gtgcgcttca gactcattct gctggtggag 5280

aacttctgtc gcttggcgcc ctgtatgtcg gccaagtagc agtttaccat gagttcgtag 5340

ttgagcgcct cggctgcgtg gcctttggcg cggagcttac ctttggaagt tttcttgcat 5400

accgggcagt ataggcattt cagcgcatac agcttgggcg caaggaaaat ggattctggg 5460

gagtatgcat ccgcgccgca ggaggcgcaa acagtttcac attccaccag ccaggttaaa 5520

tccggttcat tggggtcaaa aacaagtttt ccgccatatt ttttgatgcg tttcttacct 5580

ttggtctcca taagttcgtg tcctcgttga gtgacaaaca ggctgtccgt atctccgtag 5640

actgatttta caggcctctt ctccagtgga gtgcctcggt cttcttcgta caggaactct 5700

gaccactctg atacaaaggc gcgcgtccag gccagcacaa aggaggctat gtgggagggg 5760

tagcgatcgt tgtcaaccag ggggtccacc ttttccaaag tatgcaaaca catgtcaccc 5820

tcttcaacat ccaggaatgt gattggcttg taggtgtatt tcacgtgacc tggggtcccc 5880

gctggggggg tataaaaggg ggcggttctt tgctcttcct cactgtcttc cggatcgctg 5940

tccaggaacg tcagctgttg gggtaggtat tccctctcga aggcgggcat gacctctgca 6000

ctcaggttgt cagtttctaa gaacgaggag gatttgatat tgacagtgcc ggttgagatg 6060

cctttcatga ggttttcgtc catttggtca gaaaacacaa tttttttatt gtcaagtttg 6120

gtggcaaatg atccatacag ggcgttggat aaaagtttgg caatggatcg catggtttgg 6180

ttcttttcct tgtccgcgcg ctctttggcg gcgatgttga gttggacata ctcgcgtgcc 6240

aggcacttcc attcggggaa gatagttgtt aattcatctg gcacgattct cacttgccac 6300

cctcgattat gcaaggtaat taaatccaca ctggtggcca cctcgcctcg aaggggttca 6360

ttggtccaac agagcctacc tcctttccta gaacagaaag ggggaagtgg gtctagcata 6420

agttcatcgg gagggtctgc atccatggta aagattcccg gaagtaaatc cttatcaaaa 6480

tagctgatgg gagtggggtc atctaaggcc atttgccatt ctcgagctgc cagtgcgcgc 6540

tcatatgggt taaggggact gccccagggc atgggatggg tgagagcaga ggcatacatg 6600

ccacagatgt catagacgta gatgggatcc tcaaagatgc ctatgtaggt tggatagcat 6660

cgcccccctc tgatacttgc tcgcacatag tcatatagtt catgtgatgg cgctagcagc 6720

cccggaccca agttggtgcg attgggtttt tctgttctgt agacgatctg gcgaaagatg 6780

gcgtgagaat tggaagagat ggtgggtctt tgaaaaatgt tgaaatgggc atgaggtaga 6840

cctacagagt ctctgacaaa gtgggcataa gattcttgaa gcttggttac cagttcggcg 6900

gtgacaagta cgtctagggc gcagtagtca agtgtttctt gaatgatgtc ataacctggt 6960

tggtttttct tttcccacag ttcgcggttg agaaggtatt cttcgcgatc cttccagtac 7020

tcttctagcg gaaacccgtc tttgtctgca cggtaagatc ctagcatgta gaactgatta 7080

actgccttgt aagggcagca gcccttctct acgggtagag agtatgcttg agcagctttt 7140

cgtagcgaag cgtgagtaag ggcaaaggtg tctctgacca tgactttgag aaattggtat 7200

ttgaagtcca tgtcgtcaca ggctccctgt tcccagagtt ggaagtctac ccgtttcttg 7260

taggcggggt tgggcaaagc gaaagtaaca tcattgaaga gaatcttacc ggctctgggc 7320

ataaaattgc gagtgatgcg gaaaggctgt ggtacttccg ctcgattgtt gatcacctgg 7380

gcagctagga cgatttcgtc gaaaccgttg atgttgtgtc ctacgatgta taattctatg 7440

aaacgcggcg tgcctctgac gtgaggtagc ttactgagct catcaaaggt taggtctgtg 7500

gggtcagata aggcgtagtg ttcgagagcc cattcgtgca ggtgaggatt tgcatgtagg 7560

aatgatgacc aaagatctac cgccagtgct gtttgtaact ggtcccgata ctgacgaaaa 7620

tgccggccaa ttgccatttt ttctggagtg acacagtaga aggttctggg gtcttgttgc 7680

catcgatccc acttgagttt aatggctaga tcgtgggcca tgttgacgag acgctcttct 7740

cctgagagtt tcatgaccag catgaaagga actagttgtt tgccaaagga tcccatccag 7800

gtgtaagttt ccacatcgta ggtcaggaag agtctttctg tgcgaggatg agagccgatc 7860

gggaagaact ggatttcctg ccaccagttg gaggattggc tgttgatgtg atggaagtag 7920

aagtttctgc ggcgcgccga gcattcgtgt ttgtgcttgt acagacggcc gcagtagtcg 7980

cagcgttgca cgggttgtat ctcgtgaatg agctgtacct ggcttccctt gacgagaaat 8040

ttcagtggga agccgaggcc tggcgattgt atctcgtgct cttctatatt cgctgtatcg 8100

gcctgttcat cttctgtttc gatggtggtc atgctgacga gcccccgcgg gaggcaagtc 8160

cagacctcgg cgcgggaggg gcggagctga aggacgagag cgcgcaggct ggagctgtcc 8220

agagtcctga gacgctgcgg actcaggtta gtaggtaggg acagaagatt aacttgcatg 8280

atcttttcca gggcgtgcgg gaggttcaga tggtacttga tttccacagg ttcgtttgta 8340

gagacgtcaa tggcttgcag ggttccgtgt cctttgggcg ccactaccgt acctttgttt 8400

tttcttttga tcggtggtgg ctctcttgct tcttgcatgc tcagaagcgg tgacggggac 8460

gcgcgccggg cggcagcggt tgttccggac ccgggggcat ggctggtagt ggcacgtcgg 8520

cgccgcgcac gggcaggttc tggtattgcg ctctgagaag acttgcgtgc gccaccacgc 8580

gtcgattgac gtcttgtatc tgacgtctct gggtgaaagc taccggcccc gtgagcttga 8640

acctgaaaga gagttcaaca gaatcaattt cggtatcgtt aacggcagct tgtctcagta 8700

tttcttgtac gtcaccagag ttgtcctggt aggcgatctc cgccatgaac tgctcgattt 8760

cttcctcctg aagatctccg cgacccgctc tttcgacggt ggccgcgagg tcattggaga 8820

tacggcccat gagttgggag aatgcattca tgcccgcctc gttccagacg cggctgtaaa 8880

ccacggcccc ctcggagtct cttgcgcgca tcaccacctg agcgaggtta agctccacgt 8940

gtctggtgaa gaccgcatag ttgcataggc gctgaaaaag gtagttgagt gtggtggcaa 9000

tgtgttcggc gacgaagaaa tacatgatcc atcgtctcag cggcatttcg ctaacatcgc 9060

ccagagcttc caagcgctcc atggcctcgt agaagtccac ggcaaaatta aaaaactggg 9120

agtttcgcgc ggacacggtc aattcctcct cgagaagacg gatgagttcg gctatggtgg 9180

cccgtacttc gcgttcgaag gctcccggga tctcttcttc ctcttctatc tcttcttcca 9240

ctaacatctc ttcttcgtct tcaggcgggg gcggaggggg cacgcggcga cgtcgacggc 9300

gcacgggcaa acggtcgatg aatcgttcaa tgacctctcc gcggcggcgg cgcatggttt 9360

cagtgacggc gcggccgttc tcgcgcggtc gcagagtaaa aacaccgccg cgcatctcct 9420

taaagtggtg actgggaggt tctccgtttg ggagggagag ggcgctgatt atacatttta 9480

ttaattggcc cgtagggact gcgcgcagag atctgatcgt gtcaagatcc acgggatctg 9540

aaaacctttc gacgaaagcg tctaaccagt cacagtcaca aggtaggctg agtacggctt 9600

cttgtgggcg ggggtggtta tgtgttcggt ctgggtcttc tgtttcttct tcatctcggg 9660

aaggtgagac gatgctgctg gtgatgaaat taaagtaggc agttctaaga cggcggatgg 9720

tggcgaggag caccaggtct ttgggtccgg cttgctggat acgcaggcga ttggccattc 9780

cccaagcatt atcctgacat ctagcaagat ctttgtagta gtcttgcatg agccgttcta 9840

cgggcacttc ttcctcaccc gttctgccat gcatacgtgt gagtccaaat ccgcgcattg 9900

gttgtaccag tgccaagtca gctacgactc tttcggcgag gatggcttgc tgtacttggg 9960

taagggtggc ttgaaagtca tcaaaatcca caaagcggtg gtaagcccct gtattaatgg 10020

tgtaagcaca gttggccatg actgaccagt taactgtctg gtgaccaggg cgcacgagct 10080

cggtgtattt aaggcgcgaa taggcgcggg tgtcaaagat gtaatcgttg caggtgcgca 10140

ccagatactg gtaccctata agaaaatgcg gcggtggttg gcggtagaga ggccatcgtt 10200

ctgtagctgg agcgccaggg gcgaggtctt ccaacataag gcggtgatag ccgtagatgt 10260

acctggacat ccaggtgatt cctgcggcgg tagtagaagc ccgaggaaac tcgcgtacgc 10320

ggttccaaat gttgcgtagc ggcatgaagt agttcattgt aggcacggtt tgaccagtga 10380

ggcgcgcgca gtcattgatg ctctatagac acggagaaaa tgaaagcgtt cagcgactcg 10440

actccgtagc ctggaggaac gtgaacgggt tgggtcgcgg tgtaccccgg ttcgagactt 10500

gtactcgagc cggccggagc cgcggctaac gtggtattgg cactcccgtc tcgacccagc 10560

ctacaaaaat ccaggatacg gaatcgagtc gttttgctgg tttccgaatg gcagggaagt 10620

gagtcctatt tttttttttt ttttgccgct cagatgcatc ccgtgctgcg acagatgcgc 10680

ccccaacaac agcccccctc gcagcagcag cagcagcagc aaccacaaaa ggctgtccct 10740

gcaactactg caactgccgc cgtgagcggt gcgggacagc ccgcctatga tctggacttg 10800

gaagagggcg aaggactggc acgtctaggt gcgccttcgc ccgagcggca tccgcgagtt 10860

caactgaaaa aagattctcg cgaggcgtat gtgccccaac agaacctatt tagagacaga 10920

agcggcgagg agccggagga gatgcgagct tcccgcttta acgcgggtcg tgagctgcgt 10980

cacggtttgg accgaagacg agtgttgcga gacgaggatt tcgaagttga tgaagtgaca 11040

gggatcagtc ctgccagggc acacgtggct gcagccaacc ttgtatcggc ttacgagcag 11100

acagtaaagg aagagcgtaa cttccaaaag tcttttaata atcatgtgcg aaccctgatt 11160

gcccgcgaag aagttaccct tggtttgatg catttgtggg atttgatgga agctatcatt 11220

cagaacccta ctagcaaacc tctgaccgcc cagctgtttc tggtggtgca acacagcaga 11280

gacaatgagg ctttcagaga ggcgctgctg aacatcaccg aacccgaggg gagatggttg 11340

tatgatctta tcaacattct acagagtatc atagtgcagg agcggagcct gggcctggcc 11400

gagaaggtag ctgccatcaa ttactcggtt ttgagcttgg gaaaatatta cgctcgcaaa 11460

atctacaaga ctccatacgt tcccatagac aaggaggtga agatagatgg gttctacatg 11520

cgcatgacgc tcaaggtctt gaccctgagc gatgatcttg gggtgtatcg caatgacaga 11580

atgcatcgcg cggttagcgc cagcaggagg cgcgagttaa gcgacaggga actgatgcac 11640

agtttgcaaa gagctctgac tggagctgga accgagggtg agaattactt cgacatggga 11700

gctgacttgc agtggcagcc tagtcgcagg gctctgagcg ccgcgacggc aggatgtgag 11760

cttccttaca tagaagaggc ggatgaaggc gaggaggaag agggcgagta cttggaagac 11820

tgatggcaca acccgtgttt tttgctagat ggaacagcaa gcaccggatc ccgcaatgcg 11880

ggcggcgctg cagagccagc cgtccggcat taactcctcg gacgattgga cccaggccat 11940

gcaacgtatc atggcgttga cgactcgcaa ccccgaagcc tttagacagc aaccccaggc 12000

caaccgtcta tcggccatca tggaagctgt agtgccttcc cgatctaatc ccactcatga 12060

gaaggtcctg gccatcgtga acgcgttggt ggagaacaaa gctattcgtc cagatgaggc 12120

cggactggta tacaacgctc tcttagaacg cgtggctcgc tacaacagta gcaatgtgca 12180

aaccaatttg gaccgtatga taacagatgt acgcgaagcc gtgtctcagc gcgaaaggtt 12240

ccagcgtgat gccaacctgg gttcgctggt ggcgttaaat gctttcttga gtactcagcc 12300

tgctaatgtg ccgcgtggtc aacaggatta tactaacttt ttaagtgctt tgagactgat 12360

ggtatcagaa gtacctcaga gcgaagtgta tcagtccggt cctgattact tctttcagac 12420

tagcagacag ggcttgcaga cggtaaatct gagccaagct tttaaaaacc ttaaaggttt 12480

gtggggagtg catgccccgg taggagaaag agcaaccgtg tctagcttgt taactccgaa 12540

ctcccgcctg ttattactgt tggtagctcc tttcaccgac agcggtagca tcgaccgtaa 12600

ttcctatttg ggttacctac taaacctgta tcgcgaagcc atagggcaaa gtcaggtgga 12660

cgagcagacc tatcaagaaa ttacccaagt cagtcgcgct ttgggacagg aagacactgg 12720

cagtttggaa gccactctga acttcttgct taccaatcgg tctcaaaaga tccctcctca 12780

atatgctctt actgcggagg aggagaggat ccttagatat gtgcagcaga gcgtgggatt 12840

gtttctgatg caagaggggg caactccgac tgcagcactg gacatgacag cgcgaaatat 12900

ggagcccagc atgtatgcca gtaaccgacc tttcattaac aaactgctgg actacttgca 12960

cagagctgcc gctatgaact ctgattattt caccaatgcc atcttaaacc cgcactggct 13020

gcccccacct ggtttctaca cgggcgaata tgacatgccc gaccctaatg acggatttct 13080

gtgggacgac gtggacagcg atgttttttc acctctttct gatcatcgca cgtggaaaaa 13140

ggaaggcggt gatagaatgc attcttctgc atcgctgtcc ggggtcatgg gtgctaccgc 13200

ggctgagccc gagtctgcaa gtccttttcc tagtctaccc ttttctctac acagtgtacg 13260

tagcagcgaa gtgggtagaa taagtcgccc gagtttaatg ggcgaagagg agtacctaaa 13320

cgattccttg ctcagaccgg caagagaaaa aaatttccca aacaatggaa tagaaagttt 13380

ggtggataaa atgagtagat ggaagactta tgctcaggat cacagagacg agcctgggat 13440

catggggact acaagtagag cgagccgtag acgccagcgc catgacagac agaggggtct 13500

tgtgtgggac gatgaggatt cggccgatga tagcagcgtg ttggacttgg gtgggagagg 13560

aaggggcaac ccgtttgctc atttgcgccc tcgcttgggt ggtatgttgt gaaaaaaaat 13620

aaaaaagaaa aactcaccaa ggccatggcg acgagcgtac gttcgttctt ctttattatc 13680

tgtgtctagt ataatgaggc gagtcgtgct aggcggagcg gtggtgtatc cggagggtcc 13740

tcctccttcg tacgagagcg tgatgcagca gcagcaggcg acggcggtga tgcaatcccc 13800

actggaggct ccctttgtgc ctccgcgata cctggcacct acggagggca gaaacagcat 13860

tcgttactcg gaactggcac ctcagtacga taccaccagg ttgtatctgg tggacaacaa 13920

gtcggcggac attgcttctc tgaactatca gaatgaccac agcaacttct tgaccacggt 13980

ggtgcagaac aatgacttta cccctacgga agccagcacc cagaccatta actttgatga 14040

acgatcgcgg tggggcggtc agctaaagac catcatgcat actaacatgc caaacgtgaa 14100

cgagtatatg tttagtaaca agttcaaagc gcgtgtgatg gtgtccagaa aacctcccga 14160

cggtgctgca gttggggata cttatgatca caagcaggat attttggaat atgagtggtt 14220

cgagtttact ttgccagaag gcaacttttc agttactatg actattgatt tgatgaacaa 14280

tgccatcata gataattact tgaaagtggg tagacagaat ggagtgcttg aaagtgacat 14340

tggtgttaag ttcgacacca ggaacttcaa gctgggatgg gatcccgaaa ccaagttgat 14400

catgcctgga gtgtatacgt atgaagcctt ccatcctgac attgtcttac tgcctggctg 14460

cggagtggat tttaccgaga gtcgtttgag caaccttctt ggtatcagaa aaaaacagcc 14520

atttcaagag ggttttaaga ttttgtatga agatttagaa ggtggtaata ttccggccct 14580

cttggatgta gatgcctatg agaacagtaa gaaagaacaa aaagccaaaa tagaagctgc 14640

tacagctgct gcagaagcta aggcaaacat agttgccagc gactctacaa gggttgctaa 14700

cgctggagag gtcagaggag acaattttgc gccaacacct gttccgactg cagaatcatt 14760

attggccgat gtgtctgatg gaacggacgt gaaactcact attcaacctg tagaaaaaga 14820

tagtaagaat agaagctata atgtgttgga agacaaaatc aacacagcct atcgcagttg 14880

gtatctttcg tacaattatg gcgatcccga aaaaggagtg cgttcctgga cattgctcac 14940

cacctcagat gtcacctgcg gagcagagca ggtttactgg tcgcttccag acatgatgaa 15000

ggatcctgtc actttccgct ccactagaca agtcagtaac taccctgtgg tgggtgcaga 15060

gcttatgccc gtcttctcaa agagcttcta caacgaacaa gctgtgtact cccagcagct 15120

ccgccagtcc acctcgctta cgcacgtctt caaccgcttt cctgagaacc agattttaat 15180

ccgtccgccg gcgcccacca ttaccaccgt cagtgaaaac gttcctgctc tcacagatca 15240

cgggaccctg ccgttgcgca gcagtatccg gggagtccaa cgtgtgaccg ttactgacgc 15300

cagacgccgc acctgtccct acgtgtacaa ggcactgggc atagtcgcac cgcgcgtcct 15360

ttcaagccgc actttctaaa aaaaaaatgt ccattcttat ctcgcccagt aataacaccg 15420

gttggggtct gcgcgctcca agcaagatgt acggaggcgc acgcaaacgt tctacccaac 15480

atcccgtgcg tgttcgcgga cattttcgcg ctccatgggg tgccctcaag ggccgcactc 15540

gcgttcgaac caccgtcgat gatgtaatcg atcaggtggt tgccgacgcc cgtaattata 15600

ctcctactgc gcctacatct actgtggatg cagttattga cagtgtagtg gctgacgctc 15660

gcaactatgc tcgacgtaag agccggcgaa ggcgcattgc cagacgccac cgagctacca 15720

ctgccatgcg agccgcaaga gctctgctac gaagagctag acgcgtgggg cgaagagcca 15780

tgcttagggc ggccagacgt gcagcttcgg gcgccagcgc cggcaggtcc cgcaggcaag 15840

cagccgctgt cgcagcggcg actattgccg acatggccca atcgcgaaga ggcaatgtat 15900

actgggtgcg tgacgctgcc accggtcaac gtgtacccgt gcgcacccgt ccccctcgca 15960

cttagaagat actgagcagt ctccgatgtt gtgtcccagc ggcgaggatg tccaagcgca 16020

aatacaagga agaaatgctg caggttatcg cacctgaagt ctacggccaa ccgttgaagg 16080

atgaaaaaaa accccgcaaa atcaagcggg ttaaaaagga caaaaaagaa gaggaagatg 16140

gcgatgatgg gctggcggag tttgtgcgcg agtttgcccc acggcgacgc gtgcaatggc 16200

gtgggcgcaa agttcgacat gtgttgagac ctggaacttc ggtggtcttt acacccggcg 16260

agcgttcaag cgctactttt aagcgttcct atgatgaggt gtacggggat gatgatattc 16320

ttgagcaggc ggctgaccga ttaggcgagt ttgcttatgg caagcgtagt agaataactt 16380

ccaaggatga gacagtgtca atacccttgg atcatggaaa tcccacccct agtcttaaac 16440

cggtcacttt gcagcaagtg ttacccgtaa ctccgcgaac aggtgttaaa cgcgaaggtg 16500

aagatttgta tcccactatg caactgatgg tacccaaacg ccagaagttg gaggacgttt 16560

tggagaaagt aaaagtggat ccagatattc aacctgaggt taaagtgaga cccattaagc 16620

aggtagcgcc tggtctgggg gtacaaactg tagacattaa gattcccact gaaagtatgg 16680

aagtgcaaac tgaacccgca aagcctactg ccacctccac tgaagtgcaa acggatccat 16740

ggatgcccat gcctattaca actgacgccg ccggtcccac tcgaagatcc cgacgaaagt 16800

acggtccagc aagtctgttg atgcccaatt atgttgtaca cccatctatt attcctactc 16860

ctggttaccg aggcactcgc tactatcgca gccgaaacag tacctcccgc cgtcgccgca 16920

agacacctgc aaatcgcagt cgtcgccgta gacgcacaag caaaccgact cccggcgccc 16980

tggtgcggca agtgtaccgc aatggtagtg cggaaccttt gacactgccg cgtgcgcgtt 17040

accatccgag tatcatcact taatcaatgt tgccgctgcc tccttgcaga tatggccctc 17100

acttgtcgcc ttcgcgttcc catcactggt taccgaggaa gaaactcgcg ccgtagaaga 17160

gggatgttgg gacgcggaat gcgacgctac aggcgacggc gtgctatccg caagcaattg 17220

cggggtggtt ttttaccagc cttaattcca attatcgctg ctgcaattgg cgcgatacca 17280

ggcatagctt ccgtggcggt tcaggcctcg caacgacatt gacattggaa aaaaaacgta 17340

taaataaaaa aaaatacaat ggactctgac actcctggtc ctgtgactat gttttcttag 17400

agatggaaga catcaatttt tcatccttgg ctccgcgaca cggcacgaag ccgtacatgg 17460

gcacctggag cgacatcggc acgagccaac tgaacggggg cgccttcaat tggagcagta 17520

tctggagcgg gcttaaaaat tttggctcaa ccataaaaac atacgggaac aaagcttgga 17580

acagcagtac aggacaggcg cttagaaata aacttaaaga ccagaacttc caacaaaaag 17640

tagtcgatgg gatagcttcc ggcatcaatg gagtggtaga tttggctaac caggctgtgc 17700

agaaaaagat aaacagtcgt ttggacccgc cgccagcaac cccaggtgaa atgcaagtgg 17760

aggaagaaat tcctccgcca gaaaaacgag gcgacaagcg tccgcgtccc gatttggaag 17820

agacgctggt gacgcgcgta gatgaaccgc cttcttatga ggaagcaacg aagcttggaa 17880

tgcccaccac tagaccgata gccccaatgg ccaccggggt gatgaaacct tctcagttgc 17940

atcgacccgt caccttggat ttgccccctc cccctgctgc tactgctgta cccgcttcta 18000

agcctgtcgc tgccccgaaa ccagtcgccg tagccaggtc acgtcccggg ggcgctcctc 18060

gtccaaatgc gcactggcaa aatactctga acagcatcgt gggtctaggc gtgcaaagtg 18120

taaaacgccg tcgctgcttt taattaaata tggagtagcg cttaacttgc ctatctgtgt 18180

atatgtgtca ttacacgccg tcacagcagc agaggaaaaa aggaagaggt cgtgcgtcga 18240

cgctgagtta ctttcaagat ggccacccca tcgatgctgc cccaatgggc atacatgcac 18300

atcgccggac aggatgcttc ggagtacctg agtccgggtc tggtgcagtt cgcccgcgcc 18360

acagacacct acttcaatct gggaaataag tttagaaatc ccaccgtagc gccgacccac 18420

gatgtgacca ccgaccgtag ccagcggctc atgttgcgct tcgtgcccgt tgaccgggag 18480

gacaatacat actcttacaa agtgcggtac accctggccg tgggcgacaa cagagtgctg 18540

gatatggcca gcacgttctt tgacattagg ggcgtgttgg acagaggtcc cagtttcaaa 18600

ccctattctg gtacggctta caactctctg gctcctaaag gcgctccaaa tgcatctcaa 18660

tggattgcaa aaggcgtacc aactgcagca gccgcaggca atggtgaaga agaacatgaa 18720

acagaggaga aaactgctac ttacactttt gccaatgctc ctgtaaaagc cgaggctcaa 18780

attacaaaag agggcttacc aataggtttg gagatttcag ctgaaaacga atctaaaccc 18840

atctatgcag ataaacttta tcagccagaa cctcaagtgg gagatgaaac ttggactgac 18900

ctagacggaa aaaccgaaga gtatggaggc agggctctaa agcctactac taacatgaaa 18960

ccctgttacg ggtcctatgc gaagcctact aatttaaaag gtggtcaggc aaaaccgaaa 19020

aactcggaac cgtcgagtga aaaaattgaa tatgatattg acatggaatt ttttgataac 19080

tcatcgcaaa gaacaaactt cagtcctaaa attgtcatgt atgcagaaaa tgtaggtttg 19140

gaaacgccag acactcatgt agtgtacaaa cctggaacag aagacacaag ttccgaagct 19200

aatttgggac aacagtctat gcccaacaga cccaactaca ttggcttcag agataacttt 19260

attggactca tgtactataa cagtactggt aacatggggg tgctggctgg tcaagcgtct 19320

cagttaaatg cagtggttga cttgcaggac agaaacacag aactttctta ccaactcttg 19380

cttgactctc tgggcgacag aaccagatac tttagcatgt ggaatcaggc tgtggacagt 19440

tatgatcctg atgtacgtgt tattgaaaat catggtgtgg aagatgaact tcccaactat 19500

tgttttccac tggacggcat aggtgttcca acaaccagtt acaaatcaat agttccaaat 19560

ggagaagata ataataattg gaaagaacct gaagtaaatg gaacaagtga gatcggacag 19620

ggtaatttgt ttgccatgga aattaacctt caagccaatc tatggcgaag tttcctttat 19680

tccaatgtgg ctctgtatct cccagactcg tacaaataca ccccgtccaa tgtcactctt 19740

ccagaaaaca aaaacaccta cgactacatg aacgggcggg tggtgccgcc atctctagta 19800

gacacctatg tgaacattgg tgccaggtgg tctctggatg ccatggacaa tgtcaaccca 19860

ttcaaccacc accgtaacgc tggcttgcgt taccgatcta tgcttctggg taacggacgt 19920

tatgtgcctt tccacataca agtgcctcaa aaattcttcg ctgttaaaaa cctgctgctt 19980

ctcccaggct cctacactta tgagtggaac tttaggaagg atgtgaacat ggttctacag 20040

agttccctcg gtaacgacct gcgggtagat ggcgccagca tcagtttcac gagcatcaac 20100

ctctatgcta cttttttccc catggctcac aacaccgctt ccacccttga agccatgctg 20160

cggaatgaca ccaatgatca gtcattcaac gactacctat ctgcagctaa catgctctac 20220

cccattcctg ccaatgcaac caatattccc atttccattc cttctcgcaa ctgggcggct 20280

ttcagaggct ggtcatttac cagactgaaa accaaagaaa ctccctcttt ggggtctgga 20340

tttgacccct actttgtcta ttctggttct attccctacc tggatggtac cttctacctg 20400

aaccacactt ttaagaaggt ttccatcatg tttgactctt cagtgagctg gcctggaaat 20460

gacaggttac tatctcctaa cgaatttgaa ataaagcgca ctgtggatgg cgaaggctac 20520

aacgtagccc aatgcaacat gaccaaagac tggttcttgg tacagatgct cgccaactac 20580

aacatcggct atcagggctt ctacattcca gaaggataca aagatcgcat gtattcattt 20640

ttcagaaact tccagcccat gagcaggcag gtggttgatg aggtcaatta caaagacttc 20700

aaggccgtcg ccatacccta ccaacacaac aactctggct ttgtgggtta catggctccg 20760

accatgcgcc aaggtcaacc ctatcccgct aactatccct atccactcat tggaacaact 20820

gccgtaaata gtgttacgca gaaaaagttc ttgtgtgaca gaaccatgtg gcgcataccg 20880

ttctcgagca acttcatgtc tatgggggcc cttacagact tgggacagaa tatgctctat 20940

gccaactcag ctcatgctct ggacatgacc tttgaggtgg atcccatgga tgagcccacc 21000

ctgctttatc ttctcttcga agttttcgac gtggtcagag tgcatcagcc acaccgcggc 21060

atcatcgagg cagtctacct gcgtacaccg ttctcggccg gtaacgctac cacgtaagaa 21120

gcttcttgct tcttgcaaat agcagctgca accatggcct gcggatccca aaacggctcc 21180

agcgagcaag agctcagagc cattgtccaa gacctgggtt gcggacccta ttttttggga 21240

acctacgata agcgcttccc ggggttcatg gcccccgata agctcgcctg tgccattgta 21300

aatacggccg gacgtgagac ggggggagag cactggttgg ctttcggttg gaacccacgt 21360

tctaacacct gctacctttt tgatcctttt ggattctcgg atgatcgtct caaacagatt 21420

taccagtttg aatatgaggg tctcctgcgc cgcagcgctc ttgctaccaa ggaccgctgt 21480

attacgctgg aaaaatctac ccagaccgtg cagggccccc gttctgccgc ctgcggactt 21540

ttctgctgca tgttccttca cgcctttgtg cactggcctg accgtcccat ggacggaaac 21600

cccaccatga aattgctaac tggagtgcca aacaacatgc ttcattctcc taaagtccag 21660

cccaccctgt gtgacaatca aaaagcactc taccattttc ttaataccca ttcgccttat 21720

tttcgctctc atcgtacaca catcgaaagg gccactgcgt tcgaccgtat ggatgttcaa 21780

taatgactca tgtaaacaac gtgttcaata aacatcactt tattttttta catgtatcaa 21840

ggctctggat tacttattta tttacaagtc gaatgggttc tgacgagaat cagaatgacc 21900

cgcaggcagt gatacgttgc ggaactgata cttgggttgc cacttgaatt cgggaatcac 21960

caacttggga accggtatat cgggcaggat gtcactccac agctttctgg tcagctgcaa 22020

agctccaagc aggtcaggag ccgaaatctt gaaatcacaa ttaggaccag tgctctgagc 22080

gcgagagttg cggtacaccg gattgcagca ctgaaacacc atcagcgacg gatgtctcac 22140

gcttgccagc acggtgggat ctgcaatcat gcccacatcc agatcttcag cattggcaat 22200

gctgaacggg gtcatcttgc aggtctgcct acccatggcg ggcacccaat taggcttgtg 22260

gttgcaatcg cagtgcaggg ggatcagtat catcttggcc tgatcctgtc tgattcctgg 22320

atacacggct ctcatgaaag catcatattg cttgaaagcc tgctgggctt tactaccctc 22380

ggtataaaac atcccgcagg acctgctcga aaactggtta gctgcacagc cggcatcatt 22440

cacacagcag cgggcgtcat tgttggctat ttgcaccaca cttctgcccc agcggttttg 22500

ggtgattttg gttcgctcgg gattctcctt taaggctcgt tgtccgttct cgctggccac 22560

atccatctcg ataatctgct ccttctgaat cataatattg ccatgcaggc acttcagctt 22620

gccctcataa tcattgcagc catgaggcca caacgcacag cctgtacatt cccaattatg 22680

gtgggcgatc tgagaaaaag aatgtatcat tccctgcaga aatcttccca tcatcgtgct 22740

cagtgtcttg tgactagtga aagttaactg gatgcctcgg tgctcttcgt ttacgtactg 22800

gtgacagatg cgcttgtatt gttcgtgttg ctcaggcatt agtttaaaac aggttctaag 22860

ttcgttatcc agcctgtact tctccatcag cagacacatc acttccatgc ctttctccca 22920

agcagacacc aggggcaagc taatcggatt cttaacagtg caggcagcag ctcctttagc 22980

cagagggtca tctttagcga tcttctcaat gcttcttttg ccatccttct caacgatgcg 23040

cacgggcggg tagctgaaac ccactgctac aagttgcgcc tcttctcttt cttcttcgct 23100

gtcttgactg atgtcttgca tggggatatg tttggtcttc cttggcttct ttttgggggg 23160

tatcggagga ggaggactgt cgctccgttc cggagacagg gaggattgtg acgtttcgct 23220

caccattacc aactgactgt cggtagaaga acctgacccc acacggcgac aggtgttttt 23280

cttcgggggc agaggtggag gcgattgcga agggctgcgg tccgacctgg aaggcggatg 23340

actggcagaa ccccttccgc gttcgggggt gtgctccctg tggcggtcgc ttaactgatt 23400

tccttcgcgg ctggccattg tgttctccta ggcagagaaa caacagacat ggaaactcag 23460

ccattgctgt caacatcgcc acgagtgcca tcacatctcg tcctcagcga cgaggaaaag 23520

gagcagagct taagcattcc accgcccagt cctgccacca cctctaccct agaagataag 23580

gaggtcgacg catctcatga catgcagaat aaaaaagcga aagagtctga gacagacatc 23640

gagcaagacc cgggctatgt gacaccggtg gaacacgagg aagagttgaa acgctttcta 23700

gagagagagg atgaaaactg cccaaaacag cgagcagata actatcacca agatgctgga 23760

aatagggatc agaacaccga ctacctcata gggcttgacg gggaagacgc gctccttaaa 23820

catctagcaa gacagtcgct catagtcaag gatgcattat tggacagaac tgaagtgccc 23880

atcagtgtgg aagagctcag ctgcgcctac gagcttaacc ttttttcacc tcgtactccc 23940

cccaaacgtc agccaaacgg cacctgcgag ccaaatcctc gcttaaactt ttatccagct 24000

tttgctgtgc cagaagtact ggctacctat cacatctttt ttaaaaatca aaaaattcca 24060

gtctcctgcc gcgctaatcg cacccgcgcc gatgccctac tcaatctggg acctggttca 24120

cgcttacctg atatagcttc cttggaagag gttccaaaga tcttcgaggg tctgggcaat 24180

aatgagactc gggccgcaaa tgctctgcaa aagggagaaa atggcatgga tgagcatcac 24240

agcgttctgg tggaattgga aggcgataat gccagactcg cagtactcaa gcgaagcgtc 24300

gaggtcacac acttcgcata tcccgctgtc aacctgcccc ctaaagtcat gacggcggtc 24360

atggaccagt tactcattaa gcgcgcaagt cccctttcag aagacatgca tgacccagat 24420

gcctgtgatg agggtaaacc agtggtcagt gatgagcagc taacccgatg gctgggcacc 24480

gactctcccc gggatttgga agagcgtcgc aagcttatga tggccgtggt gctggttacc 24540

gtagaactag agtgtctccg acgtttcttt accgattcag aaaccttgcg caaactcgaa 24600

gagaatctgc actacacttt tagacacggc tttgtgcggc aggcatgcaa gatatctaac 24660

gtggaactca ccaacctggt ttcctacatg ggtattctgc atgagaatcg cctaggacaa 24720

agcgtgctgc acagcaccct taagggggaa gcccgccgtg attacatccg cgattgtgtc 24780

tatctctacc tgtgccacac gtggcaaacc ggcatgggtg tatggcagca atgtttagaa 24840

gaacagaact tgaaagagct tgacaagctc ttacagaaat ctcttaaggt tctgtggaca 24900

gggttcgacg agcgcaccgt cgcttccgac ctggcagacc tcatcttccc agagcgtctc 24960

agggttactt tgcgaaacgg attgcctgac tttatgagcc agagcatgct taacaatttt 25020

cgctctttca tcctggaacg ctccggtatc ctgcccgcca cctgctgcgc actgccctcc 25080

gactttgtgc ctctcaccta ccgcgagtgc cccccgccgc tatggagtca ctgctacctg 25140

ttccgtctgg ccaactatct ctcctaccac tcggatgtga tcgaggatgt gagcggagac 25200

ggcttgctgg agtgccactg ccgctgcaat ctgtgcacgc cccaccggtc cctagcttgc 25260

aacccccagt tgatgagcga aacccagata ataggcacct ttgaattgca aggccccagc 25320

agccaaggcg atgggtcttc tcctgggcaa agtttaaaac tgaccccggg actgtggacc 25380

tccgcctact tgcgcaagtt tgctccggaa gattaccacc cctatgaaat caagttctat 25440

gaggaccaat cacagcctcc aaaggccgaa ctttcggctt gcgtcatcac ccagggggca 25500

attctggccc aattgcaagc catccaaaaa tcccgccaag aatttctact gaaaaagggt 25560

aagggggtct accttgaccc ccagaccggc gaggaactca acacaaggtt ccctcaggat 25620

gtcccaacga cgagaaaaca agaagttgaa ggtgcagccg ccgcccccag aagatatgga 25680

ggaagattgg gacagtcagg cagaggaggc ggaggaggac agtctggagg acagtctgga 25740

ggaagacagt ttggaggagg aaaacgagga ggcagaggag gtggaagaag taaccgccga 25800

caaacagtta tcctcggctg cggagacaag caacagcgct accatctccg ctccgagtcg 25860

aggaacccgg cggcgtccca gcagtagatg ggacgagacc ggacgcttcc cgaacccaac 25920

cagcgcttcc aagaccggta agaaggatcg gcagggatac aagtcctggc gggggcataa 25980

gaatgccatc atctcctgct tgcatgagtg cgggggcaac atatccttca cgcggcgcta 26040

cttgctattc caccatgggg tgaactttcc gcgcaatgtt ttgcattact accgtcacct 26100

ccacagcccc tactatagcc agcaaatccc gacagtctcg acagataaag acagcggcgg 26160

cgacctccaa cagaaaacca gcagcggcag ttagaaaata cacaacaagt gcagcaacag 26220

gaggattaaa gattacagcc aacgagccag cgcaaacccg agagttaaga aatcggatct 26280

ttccaaccct gtatgccatc ttccagcaga gtcggggtca agagcaggaa ctgaaaataa 26340

aaaaccgatc tctgcgttcg ctcaccagaa gttgtttgta tcacaagagc gaagatcaac 26400

ttcagcgcac tctcgaggac gccgaggctc tcttcaacaa gtactgcgcg ctgactctta 26460

aagagtaggc agcgaccgcg cttattcaaa aaaggcggga attacatcat cctcgacatg 26520

agtaaagaaa ttcccacgcc ttacatgtgg agttatcaac cccaaatggg attggcagca 26580

ggcgcctccc aggactactc cacccgcatg aattggctca gcgccgggcc ttctatgatt 26640

tctcgagtta atgatatacg cgcctaccga aaccaaatac ttttggaaca gtcagctctt 26700

accaccacgc cccgccaaca ccttaatccc agaaattggc ccgccgccct agtgtaccag 26760

gaaagtcccg ctcccaccac tgtattactt cctcgagacg cccaggccga agtccaaatg 26820

actaatgcag gtgcgcagtt agctggcggc tccaccctat gtcgtcacag gcctcggcat 26880

aatataaaac gcctgatgat cagaggccga ggtatccagc tcaacgacga gtcggtgagc 26940

tctccgcttg gtctacgacc agacggaatc tttcagattg ccggctgcgg gagatcttcc 27000

ttcacccctc gtcaggctgt tctgactttg gaaagttcgt cttcgcaacc ccgctcgggc 27060

ggaatcggga ccgttcaatt tgtagaggag tttactccct ctgtctactt caaccccttc 27120

tccggatctc ctgggcacta cccggacgag ttcataccga acttcgacgc gattagcgag 27180

tcagtggacg gctacgattg atgtctggtg acgcggctga gctatctcgg ctgcgacatc 27240

tagaccactg ccgccgcttt cgctgctttg cccgggaact tattgagttc atctacttcg 27300

aactccccaa ggatcaccct caaggtccgg cccacggagt gcggattact atcgaaggca 27360

aaatagactc tcgcctgcaa cgaattttct cccagcggcc cgtgctgatc gagcgagacc 27420

agggaaacac cacggtttcc atctactgca tttgtaatca ccccggattg catgaaagcc 27480

tttgctgtct tatgtgtact gagtttaata aaaactgaat taagactctc ctacggactg 27540

ccgcttcttc aacccggatt ttacaaccag aagaacaaaa cttttcctgt cgtccaggac 27600

tctgttaact tcacctttcc tactcacaaa ctagaagctc aacgactaca ccgcttttcc 27660

agaagcattt tccctactaa tactactttc aaaaccggag gtgagctcca cggtctccct 27720

acagaaaacc cttgggtgga agcgggcctt gtagtactag gaattcttgc gggtgggctt 27780

gtgattattc tttgctacct atacacacct tgcttcactt tcctagtggt gttgtggtat 27840

tggtttaaaa aatggggccc atactagtct tgcttgtttt actttcgctt ttggaaccgg 27900

gttctgccaa ttacgatcca tgtctagact ttgacccaga aaactgcaca cttacttttg 27960

cacccgacac aagccgcatc tgtggagttc ttattaagtg cggatgggaa tgcaggtccg 28020

ttgaaattac acacaataac aaaacctgga acaatacctt atccaccaca tgggagccag 28080

gagttcccga gtggtacact gtctctgtcc gaggtcctga cggttccatc cgcattagta 28140

acaacacttt cattttttct gaaatgtgcg atctggccat gttcatgagc aaacagtatt 28200

ctctatggcc tcctagcaag gacaacatcg taacgttctc cattgcttat tgcttgtgcg 28260

cttgccttct tactgcttta ctgtgcgtat gcatacacct gcttgtaacc actcgcatca 28320

aaaacgccaa taacaaagaa aaaatgcctt aacctctttc tgtttacaga catggcttct 28380

cttacatctc tcatatttgt cagcattgtc actgccgctc acggacaaac agtcgtctct 28440

atcccactag gacataatta cactctcata ggacccccaa tcacttcaga ggtcatctgg 28500

accaaactgg gaagcgttga ttactttgat ataatctgta acaaaacaaa accaataata 28560

gtaacttgca acatacaaaa tcttacattg attaatgtta gcaaagttta cagcggttac 28620

tattatggtt atgacagata cagtagtcaa tatagaaatt acttggttcg tgttacccag 28680

ttgaaaacca cgaaaatgcc aaatatggca aagattcgat ccgatgacaa ttctctagaa 28740

acttttacat ctcccaccac acccgacgaa aaaaacatcc cagattcaat gattgcaatt 28800

gttgcagcgg tggcagtggt gatggcacta ataataatat gcatgctttt atatgcttgt 28860

cgctacaaaa agtttcatcc taaaaaacaa gatctcctac taaggcttaa catttaattt 28920

ctttttatac agccatggtt tccactacca cattccttat gcttactagt ctcgcaactc 28980

tgacttctgc tcgctcacac ctcactgtaa ctataggctc aaactgcaca ctaaaaggac 29040

ctcaaggtgg tcatgtcttt tggtggagaa tatatgacaa tggatggttt acaaaaccat 29100

gtgaccaacc tggtagattt ttctgcaacg gcagagacct aaccattatc aacgtgacag 29160

caaatgacaa aggcttctat tatggaaccg actataaaag tagtttagat tataacatta 29220

ttgtactgcc atctaccact ccagcacccc gcacaactac tttctctagc agcagtgtcg 29280

ctaacaatac aatttccaat ccaacctttg ccgcgctttt aaaacgcact gtgaataatt 29340

ctacaacttc acatacaaca atttccactt caacaatcag catcatcgct gcagtgacaa 29400

ttggaatatc tattcttgtt tttaccataa cctactacgc ctgctgctat agaaaagaca 29460

aacataaagg tgatccatta cttagatttg atatttaatt tgttcttttt ttttatttac 29520

agtatggtga acaccaatca tggtacctag aaatttcttc ttcaccatac tcatctgtgc 29580

ttttaatgtt tgcgctactt tcacagcagt agccacagca accccagact gtataggagc 29640

atttgcttcc tatgcacttt ttgcttttgt tacttgcatc tgcgtatgta gcatagtctg 29700

cctggttatt aattttttcc aacttctaga ctggatcctt gtgcgaattg cctacctgcg 29760

ccaccatccc gaataccgca accaaaatat cgcggcactt cttagactca tctaaaacca 29820

tgcaggctat actaccaata tttttgcttc tattgcttcc ctacgctgtc tcaaccccag 29880

ctgcctatag tactccacca gaacacctta gaaaatgcaa attccaacaa ccgtggtcat 29940

ttcttgcttg ctatcgagaa aaatcagaaa tccccccaaa tttaataatg attgctggaa 30000

taattaatat aatctgttgc accataattt catttttgat atacccccta tttgattttg 30060

gctggaatgc tcccaatgca catgatcatc cacaagaccc agaggaacac attcccccac 30120

aaaacatgca acatccaata gcgctaatag attacgaaag tgaaccacaa cccccactac 30180

tccctgctat tagttacttc aacctaaccg gcggagatga ctgaaacact caccacctcc 30240

aattccgccg aggatctgct cgatatggac ggccgcgtct cagaacaacg acttgcccaa 30300

ctacgcatcc gccagcagca ggaacgcgtg gccaaagagc tcagagatgt catccaaatt 30360

caccaatgca aaaaaggcat attctgtttg gtaaaacaag ccaagatatc ctacgagatc 30420

accgctactg accatcgcct ctcttacgaa cttggccccc aacgacaaaa atttacctgc 30480

atggtgggaa tcaaccccat agttatcacc caacaaagtg gagatactaa gggttgcatt 30540

cactgctcct gcgattccat cgagtgcacc tacaccctgc tgaagaccct atgcggccta 30600

agagacctgc taccaatgaa ttaaaaaaaa atgattaata aaaaatcact tacttgaaat 30660

cagcaataag gtctctgttg aaattttctc ccagcagcac ctcacttccc tcttcccaac 30720

tctggtattc taaaccccgt tcagcggcat actttctcca tactttaaag gggatgtcaa 30780

attttagctc ctctcctgta cccacaatct tcatgtcttt cttcccagat gaccaagaga 30840

gtccggctca gtgactcctt caaccctgtc tacccctatg aagatgaaag cacctcccaa 30900

caccccttta taaacccagg gtttatttcc ccaaatggct tcacacaaag cccagacgga 30960

gttcttactt taaaatgttt aaccccacta acaaccacag gcggatctct acagctaaaa 31020

gtgggagggg gacttacagt ggatgacact gatggtacct tacaagaaaa catacgtgct 31080

acagcaccca ttactaaaaa taatcactct gtagaactat ccattggaaa tggattagaa 31140

actcaaaaca ataaactatg tgccaaattg ggaaatgggt taaaatttaa caacggtgac 31200

atttgtataa aggatagtat taacacctta tggactggaa taaaccctcc acctaactgt 31260

caaattgtgg aaaacactaa tacaaatgat ggcaaactta ctttagtatt agtaaaaaat 31320

ggagggcttg ttaatggcta cgtgtctcta gttggtgtat cagacactgt gaaccaaatg 31380

ttcacacaaa agacagcaaa catccaatta agattatatt ttgactcttc tggaaatcta 31440

ttaactgagg aatcagactt aaaaattcca cttaaaaata aatcttctac agcgaccagt 31500

gaaactgtag ccagcagcaa agcctttatg ccaagtacta cagcttatcc cttcaacacc 31560

actactaggg atagtgaaaa ctacattcat ggaatatgtt actacatgac tagttatgat 31620

agaagtctat ttcccttgaa catttctata atgctaaaca gccgtatgat ttcttccaat 31680

gttgcctatg ccatacaatt tgaatggaat ctaaatgcaa gtgaatctcc agaaagcaac 31740

atagctacgc tgaccacatc cccctttttc ttttcttaca ttacagaaga cgacaactaa 31800

aataaagttt aagtgttttt atttaaaatc acaaaattcg agtagttatt ttgcctccac 31860

cttcccattt gacagaatac accaatctct ccccacgcac agctttaaac atttggatac 31920

cattagagat agacattgtt ttagattcca cattccaaac agtttcagag cgagccaatc 31980

tggggtcagt gatagataaa aatccatcgc gatagtcttt taaagcgctt tcacagtcca 32040

actgctgcgg atgcgactcc ggagtttgga tcacggtcat ctggaagaag aacgatggga 32100

atcataatcc gaaaacggta tcggacgatt gtgtctcatc aaacccacaa gcagccgctg 32160

tctgcgtcgc tccgtgcgac tgctgtttat gggatcaggg tccacagttt cctgaagcat 32220

gattttaata gcccttaaca tcaactttct ggtgcgatgc gcgcagcaac gcattctgat 32280

ttcactcaaa tctttgcagt aggtacaaca cattattaca atattgttta ataaaccata 32340

attaaaagcg ctccagccaa aactcatatc tgatataatc gcccctgcat gaccatcata 32400

ccaaagttta atataaatta aatgacgttc cctcaaaaac acactaccca catacatgat 32460

ctcttttggc atgtgcatat taacaatctg tctgtaccat ggacaacgtt ggttaatcat 32520

gcaacccaat ataaccttcc ggaaccacac tgccaacacc gctcccccag ccatgcattg 32580

aagtgaaccc tgctgattac aatgacaatg aagaacccaa ttctctcgac cgtgaatcac 32640

ttgagaatga aaaatatcta tagtggcaca acatagacat aaatgcatgc atcttctcat 32700

aatttttaac tcctcaggat ttagaaacat atcccaggga ataggaagct cttgcagaac 32760

agtaaagctg gcagaacaag gaagaccacg aacacaactt acactatgca tagtcatagt 32820

atcacaatct ggcaacagcg ggtggtcttc agtcatagaa gctcgggttt cattttcctc 32880

acaacgtggt aactgggctc tggtgtaagg gtgatgtctg gcgcatgatg tcgagcgtgc 32940

gcgcaacctt gtcataatgg agttgcttcc tgacattctc gtattttgta tagcaaaacg 33000

cggccctggc agaacacact cttcttcgcc ttctatcctg ccgcttagcg tgttccgtgt 33060

gatagttcaa gtacagccac actcttaagt tggtcaaaag aatgctggct tcagttgtaa 33120

tcaaaactcc atcgcatcta attgttctga ggaaatcatc cacggtagca tatgcaaatc 33180

ccaaccaagc aatgcaactg gattgcgttt caagcaggag aggagaggga agagacggaa 33240

gaaccatgtt aatttttatt ccaaacgatc tcgcagtact tcaaattgta gatcgcgcag 33300

atggcatctc tcgcccccac tgtgttggtg aaaaagcaca gctaaatcaa aagaaatgcg 33360

attttcaagg tgctcaacgg tggcttccaa caaagcctcc acgcgcacat ccaagaacaa 33420

aagaatacca aaagaaggag cattttctaa ctcctcaatc atcatattac attcctgcac 33480

cattcccaga taattttcag ctttccagcc ttgaattatt cgtgtcagtt cttgtggtaa 33540

atccaatcca cacattacaa acaggtcccg gagggcgccc tccaccacca ttcttaaaca 33600

caccctcata atgacaaaat atcttgctcc tgtgtcacct gtagcgaatt gagaatggca 33660

acatcaattg acatgccctt ggctctaagt tcttctttaa gttctagttg taaaaactct 33720

ctcatattat caccaaactg cttagccaga agccccccgg gaacaagagc aggggacgct 33780

acagtgcagt acaagcgcag acctccccaa ttggctccag caaaaacaag attggaataa 33840

gcatattggg aaccaccagt aatatcatcg aagttgctgg aaatataatc aggcagagtt 33900

tcttgtagaa attgaataaa agaaaaattt gccaaaaaaa cattcaaaac ctctgggatg 33960

caaatgcaat aggttaccgc gctgcgctcc aacattgtta gttttgaatt agtctgcaaa 34020

aataaaaaaa aaacaagcgt catatcatag tagcctgacg aacaggtgga taaatcagtc 34080

tttccatcac aagacaagcc acagggtctc cagctcgacc ctcgtaaaac ctgtcatcgt 34140

gattaaacaa cagcaccgaa agttcctcgc ggtgaccagc atgaataagt cttgatgaag 34200

catacaatcc agacatgtta gcatcagtta aggagaaaaa acagccaaca tagcctttgg 34260

gtataattat gcttaatcgt aagtatagca aagccacccc tcgcggatac aaagtaaaag 34320

gcacaggaga ataaaaaata taattatttc tctgctgctg tttaggcaac gtcgcccccg 34380

gtccctctaa atacacatac aaagcctcat cagccatggc ttaccagaga aagtacagcg 34440

ggcacacaaa ccacaagctc taaagtcact ctccaacctc tccacaatat atatacacaa 34500

gccctaaact gacgtaatgg gactaaagtg taaaaaatcc cgccaaaccc aacacacacc 34560

ccgaaactgc gtcaccaggg aaaagtacag tttcacttcc gcaatcccaa caagcgtcac 34620

ttcctctttc tcacggtacg tcacatccca ttaacttaca acgtcatttt cccacggccg 34680

cgccgcccct tttaaccgtt aaccccacag ccaatcacca cacggcccac actttttaaa 34740

atcacctcat ttacatattg gcaccattcc atctataagg tatattattg atgatg 34796