(en)The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

1.ApplicationNumber: US-18037502-A
1.PublishNumber: US-2003126638-A1
2.Date Publish: 20030703
3.Inventor: ALLEN WILLIAM B.
CAHOON REBECCA E.
FAMODU OMOLAYO O.
HARVELL LESLIE T.
HELENTJARIS TIMOTHY G.
LI CHANGJIANG
LOWE KEITH S.
OLIVEIRA IGOR CUNHA
SHEN BO
TARCZYNSKI MITCHELL C.

4.Inventor Harmonized: ALLEN WILLIAM B(US)
CAHOON REBECCA E(US)
FAMODU OMOLAYO O(US)
HARVELL LESLIE T(US)
HELENTJARIS TIMOTHY G(US)
LI CHANGJIANG(US)
LOWE KEITH S(US)
OLIVEIRA IGOR CUNHA(US)
SHEN BO(US)
TARCZYNSKI MITCHELL C(US)

5.Country: US
6.Claims:
(en)The preparation and use of nucleic acid fragments useful in altering the oil phenotype in plants are disclosed. Recombinant DNA construct incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic plants having altered lipid profiles. Methods for altering the oil phenotype in plants using such nucleic acid fragments also are disclosed.

7.Description:
(en)[0001] This application claims the priority benefit of U.S. Provisional Application 60/301,913 filed Jun. 29, 2001, the disclosure of which is hereby incorporated by reference in its entirety.



FIELD OF THE INVENTION
[0002] The present invention is in the field of plant breeding and genetics and, in particular, relates to the alteration of oil phenotype in plants through the controlled expression of selective genes.
BACKGROUND OF THE INVENTION
[0003] Plant lipids have a variety of industrial and nutritional uses and are central to plant membrane function and climatic adaptation. These lipids represent a vast array of chemical structures, and these structures determine the physiological and industrial properties of the lipid. Many of these structures result either directly or indirectly from metabolic processes that alter the degree of unsaturation of the lipid. Different metabolic regimes in different plants produce these altered lipids, and either domestication of exotic plant species or modification of agronomically adapted species is usually required to produce economically large amounts of the desired lipid.
[0004] There are serious limitations to using mutagenesis to alter fatty acid composition. Screens will rarely uncover mutations that a) result in a dominant (“gain-of-function”) phenotype, b) are in genes that are essential for plant growth, and c) are in an enzyme that is not rate-limiting and that is encoded by more than one gene. In cases where desired phenotypes are available in mutant corn lines, their introgression into elite lines by traditional breeding techniques is slow and expensive, since the desired oil compositions are likely the result of several recessive genes.
[0005] Recent molecular and cellular biology techniques offer the potential for overcoming some of the limitations of the mutagenesis approach, including the need for extensive breeding. Some of the particularly useful technologies are seed-specific expression of foreign genes in transgenic plants [see Goldberg et al (1989) Cell 56:149-160], and the use of antisense RNA to inhibit plant target genes in a dominant and tissue-specific manner [see van der Krol et al (1988) Gene 72:45-50]. Other advances include the transfer of foreign genes into elite commercial varieties of commercial oilcrops, such as soybean [Chee et al (1989) Plant Physiol. 91:1212-1218; Christou et al (1989) Proc. Natl. Acad. Sci. U.S.A. 86:7500-7504; Hinchee et al (1988) Bio/Technology 6:915-922; EPO publication 0 301 749 A2], rapeseed [De Block et al (1989) Plant Physiol. 91:694-701], and sunflower [Everett et al(1987) Bio/Technology 5:1201-1204], and the use of genes as restriction fragment length polymorphism (RFLP) markers in a breeding program, which makes introgression of recessive traits into elite lines rapid and less expensive [Tanksley et al (1989) Bio/Technology 7:257-264]. However, application of each of these technologies requires identification and isolation of commercially-important genes.
[0006] The regulation of transcription of most eukaryotic genes is coordinated through sequence-specific binding of proteins to the promoter region located upstream of the gene. Many of these protein-binding sequences have been conserved during evolution and are found in a wide variety of organisms. One such feature is the “CCAAT” sequence element. (Edwards et al, 1998, Plant Physiol. 117:1015-1022). CCAAT boxes are a feature of gene promoters in many eukaryotes including several plant gene promoters.
[0007] HAP proteins constitute a large family of transcription factors first identified in yeast. They combine to from a heteromeric protein complex that activates transcription by binding to CCAAT boxes in eukaryotic promoters. The orthologous Hap proteins display a high degree of evolutionary conservation in their functional domains in all species studied to date (Li et al, 1991).
[0008] WO 00/28058 published on May 18, 2000 describes Hap3-type CCAAT-box binding transcriptional activator polynucleotides and polypeptides, especially, the leafy cotyledon 1 transcriptional activator (LEC1) polynucleotides and polypeptides.
[0009] WO 99/67405 describes leafy cotyledons 1 genes and their uses.
[0010] The human, murine and plant homologues of CCAAT-binding proteins have been isolated and characterized based on their sequence similarity with their yeast counterparts (Li et al, 1991). This high degree of sequence homology translates remarkably into functional interchangeability among orthologue proteins of different species (Sinha et al, 1995). Unlike yeast, multiple forms of each HAP homolog have been identified in plants (Edwards et al, 1998).
[0011] Molecular and genetic analysis revealed HAP members to be involved in the control of diverse and critical biological processes ranging from development and cell cycle regulation to metabolic control and homeostasis (Lotan et al, 1998; Lopez et al, 1996). In yeast, HAPs are involved in the transcriptional control of metabolic relevant processes such as the regulation of catabolic derepression of cyc1 and other genes involved in respiration (Becker et al., 1991).
[0012] In mammalian systems, several reports describe HAPs as direct or indirect regulators of several important genes involved in lipid biosynthesis such as fatty acid synthase (Roder et al, 1997), farnesyl diphosphate (FPP) synthase (Jackson et al, 1995; Ericsson et al, 1996), glycerol-3-phosphate acyltransferase (GPA, Jackson et al, 1997), acetyl-CoA carboxylase (ACC, Lopez et al, 1996) and 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (Jackson et al, 1995), among others.
[0013] In addition, other CCAAT-binding transcription factors have also been reported to be involved in different aspects of the control of lipid biosynthesis and adipocyte growth and differentiation in mammalian systems (see McKnight et al, 1989).
[0014] It appears that the currently available evidence to date points to a family of proteins of the CCAAT-binding transcription factors as important modulators of metabolism and lipid biosynthesis in mammalian systems. Such a determination has not been made for plant systems.
SUMMARY OF THE INVENTION
[0015] This invention concerns an isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
[0016] (a) a nucleic acid sequence encoding a fifth polypeptide having Hap2-like transcription factor activity, the fifth polypeptide having at least 70% identity based on the Clustal method of alignment when compared to a sixth polypeptide selected from the group consisting of SEQ ID NOs: 2, 4, 5, 6,10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38,40, 42, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, or 208, 210, 212, 214, or 216;
[0017] (b) a nucleic acid sequence encoding a seventh polypeptide having Hap5-like transcription factor activity, the seventh polypeptide having at least 80% identity based on the Clustal method of alignment when compared to an eighth polypeptide selected from the group consisting of SEQ ID NOs: 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, or 221;
[0018] (c) a nucleic acid sequence encoding a seventeenth polypeptide having Hap3/Lec1-like activity, the seventeenth polypeptide having at least 70% identity based on the Clustal method of alignment when compared to a eighteenth polypeptide selected from the group consisting of SEQ ID NOs: 130, 132, 134, or 136.
[0019] Also of interest are the complements of such nucleotide fragment as well as the use of such fragments or a part thereof in antisense inhibition or co-suppression in a transformed plant.
[0020] In a second embodiment, this invention concerns recombinant DNA constructs comprising such fragments, plants comprising such recombinant DNA constructs in their genome, seeds obtained from such plants and oil obtained from these seeds.
[0021] In a third embodiment, this invention concerns a method for altering oil phenotype in a plant which comprises: (a) transforming a plant with a recombinant DNA construct the invention, (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0022] In a fourth embodiment, this invention concerns a method for altering oil phenotype in a plant which comprises:
[0023] (a) transforming a plant with a recombinant DNA construct comprising isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
[0024] (i) a nucleic acid sequence encoding a plant Hap3/Lec1 transcription factor having at least 60% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of even SEQ ID NOs: from 130 to 148, and SEQ ID NOs: 195 and 196;
[0025] (ii) the complement of the nucleic acid sequence of (i);
[0026] (iii) the sequence of (i) or (ii) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
[0027] (iv) a nucleic acid sequence encoding a plant Lec1-related CCAAT binding transcription factor having at least 60% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of even SEQ ID NOs: from 150 to 178, and SEQ ID NOs: 197 to 202;
[0028] (v) the complement of the nucleic acid sequence of (iv);
[0029] (vi) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
[0030] wherein said nucleic acid sequence is operably linked to at least one regulatory sequence;
[0031] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0032] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0033] In a fifth embodiment, this invention concerns a method for altering oil phenotype in a plant which comprises:
[0034] (a) transforming a plant with a recombinant DNA construct comprising an isolated nucleic acid fragment operably linked to at least one regulatory sequence wherein said fragment has a nucleic acid sequence encoding a polypeptide having a sequence identity of at least 60% based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of even SEQ ID NOs: from 2 to 178, and 206 to 214, and SEQ ID NOs: 179 to 202, 216 to 219, 221, and 222;
[0035] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0036] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0037] In a sixth embodiment, this invention concerns method of mapping genetic variations related to altered oil phenotypes in a plant comprising:
[0038] (a) crossing two plant varieties; and
[0039] (b) evaluating genetic variations with respect to nucleic acid sequences set forth in any one of the odd SEQ ID NOs: from 1 to 177, or 207 to 215, or SEQ ID NO: 220 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.
[0040] In a seventh embodiment, this invention concerns a method of molecular breeding to obtain altered oil phenotypes in a plant comprising:
[0041] (a) crossing two plant varieties; and
[0042] (b) evaluating genetic variations with respect to nucleic acid sequences set forth in any one of the odd SEQ ID NOs: from 1 to 177, or 207 to 215, or SEQ ID NO: 220 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.
[0043] In an eighth embodiment, this invention concerns a method for altering oil phenotype in a plant which comprises:
[0044] (a) transforming a plant with a recombinant DNA construct comprising isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
[0045] (i) a nucleic acid sequence encoding a plant Hap3/Lec1 transcription factor having at least 70% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of SEQ ID NOs: 130 to 148, and SEQ ID NOs: 195, 196, and 206;
[0046] (ii) the complement of the nucleic acid sequence of (iv);
[0047] (iii) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
[0048] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0049] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0050] In a ninth embodiment, this invention concerns a method to isolate nucleic acid fragments associated with altering oil phenotype in a plant which comprises:
[0051] (a) comparing even SEQ ID NOs: from 2 to 178, and 206 to 214, and SEQ ID NOs: 179 to 202, 216 to 219, 221, and 222 with other polypeptide sequences for the purpose of identifying polypeptides associated with altering oil phenotype in a plant;
[0052] (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);
[0053] (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b); and
[0054] (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering oil phenotype by sequence dependent protocols.



BRIEF DESCRIPTION OF THE FIGURES AND SEQUENCE LISTINGS
[0055] The invention can be more fully understood from the following detailed description and the accompanying drawings and Sequence Listing which form a part of this application.
[0056]FIG. 1 shows the fatty acid composition of maize somatic embryos over-expressing Hap3/Lec1 (solid bars, “Hap3/Lec1”) compared to control embryos (striped bars, “con”). A ubiquitin promoter was used to drive Hap3/Lec1 expression in maize embryogenic callus. More than ten different events were analyzed by GC for fatty acid content-composition and compared to controls transformed with the selectable marker (BAR gene) plasmid alone. The somatic embryos over-expressing Lec1 contain elevated fatty acid contents averaging 119% over control oil levels.
[0057]FIG. 2 shows the fatty acid composition of maize embryos transformed with additional copies of Hap3/Lec1 (solid bars, “+transgene”) compared to control embryos (cross-hatched bars, “−transgene”). An oleosin promoter was used to direct the expression of a transgenic copy of Hap3/Lec 1. More than twenty events producing segregating T1 seed were analyzed by NMR for embryo oil content. Six to twelve embryos were analyzed for each of five different events. Some embryos within each event contained elevated oil content. The same embryos from these five events were analyzed by PCR to determine the presence or absence of the Lec1 construct. Embryos with high oil were always found to contain the Lec1 construct (darkly shaded bars), whereas embryos with normal levels of oil were typically found not to contain the Lec1 construct (cross-hatched bars). These data demonstrate the presence of the Lec1 gene does lead to increased oil in the embryo. It is believed that embryos containing sharply higher levels of oil were homozygous for the Lec1 construct, as these events were segregating 1:2:1. The oil concentration in the embryos containing the Lec1 construct greatly surpassed any increase previously achieved through enzymatic modification of the fatty acid biosynthetic pathway, with some embryos containing an average increase of 56% in embryo oil content.


[0058] Table 1 lists the polypeptides that are described herein, the designation of the cDNA clones that comprise the nucleic acid fragments encoding polypeptides representing all or a substantial portion of these polypeptides (for the corresponding SEQ ID NO: identifier as used in the attached Sequence Listing see Table 3). The sequence descriptions and Sequence Listing attached hereto comply with the rules governing nucleotide and/or amino acid sequence disclosures in patent applications as set forth in 37 C.F.R. §1.821-1.825.
TABLE 1 Genes Involved in Alteration of Oil Traits in Plants Gene Name Clone Plant Hap2a transcription factor ncs.pk0013.c4 Catalpa [ Catalpa speciosa ] Hap2c-like transcription etr1c.pk006.f9 cattail [ Typha latifolia ] factor Hap2a transcription factor vmb1na.pk015.d18:fis grape [Vitis sp.] Hap2a transcription factor vpl1c.pk008.o5:fis grape [Vitis sp.] Hap2c-like transcription vdb1c.pk001.m5:fis grape [Vitis sp.] factor Hap2 transcription factor cho1c.pk004.b19:fis maize [ Zea mays ] Hap2 transcription factor p0015.cdpgu90r:fis maize [ Zea mays ] Hap2a transcription factor cta1n.pk0010.f3:fis maize [ Zea mays ] Hap2a-like transcription cco1n.pk0014.d4:fis maize [ Zea mays ] factor Hap2a-like transcription cco1n.pk086.d20:fis maize [ Zea mays ] factor Hap2b transcription factor p0126.cnlau71r:fis maize [ Zea mays ] Hap2b-like transcription p0104.cabav52r maize [ Zea mays ] factor Hap2c transcription factor cho1c.pk007.l21:fis maize [ Zea mays ] Hap2c-like transcription contig of: maize [ Zea mays ] factor cca.pk0026.d6 cen3n.pk0061.e10:fis cen3n.pk0135.c2 cho1c.pk001.n24 p0092.chwae40r Hap2c-like transcription cpf1c.pk006.e3:fis maize [ Zea mays ] factor Hap2c-like transcription contig of: maize [ Zea mays ] factor cr1n.pk0080.g6 p0003.cgpge51r Hap2c-like transcription p0015.cpdfm55r:fis maize [ Zea mays ] factor Hap2c-like transcription p0083.cldct11r:fis maize [ Zea mays ] factor Hap2c-like transcription p0083.cldeu68r:fis maize [ Zea mays ] factor Hap2a transcription factor pps1c.pk001.h3:fis prickly poppy [ Argemone mexicana ] Hap2c-like transcription pps1c.pk007.j21:fis prickly poppy factor [ Argemone mexicana ] Hap2 transcription factor rr1.pk0030.f7:fis rice [ Oryza sativa ] Hap2a transcription factor r1s72.pk0023.c8:fis rice [ Oryza sativa ] Hap2a-like transcription rca1n.pk002.c15 rice [ Oryza sativa ] factor Hap2a-like transcription rds3c.pk001.g9 rice [ Oryza sativa ] factor Hap2b transcription factor rca1n.pk002.j3:fis rice [ Oryza sativa ] Hap2c-like transcription rca1n.pk029.n22:fis rice [ Oryza sativa ] factor Hap2c-like transcription rl0n.pk131.j17 rice [ Oryza sativa ] factor Hap2a transcription factor sdp3c.pk018.b9:fis soybean [ Glycine max ] Hap2a transcription factor sfl1.pk0102.h8 soybean [ Glycine max ] Hap2a transcription factor srr3c.pk001.l10:fis soybean [ Glycine max ] Hap2a-like transcription sdp2c.pk003.o5:fis soybean [ Glycine factor max ] Hap2b transcription factor sif1c.pk001.m16:fis soybean [ Glycine max ] Hap2c-like transcription src1c.pk003.o16:fis soybean [ Glycine factor max ] Hap2c-like transcription src3c.pk012.m6:fis soybean [ Glycine factor max ] Hap2c-like transcription hss1c.pk011.h10:fis sunflower [Helianthus factor sp.] Hap2 transcription factor wr1.pk0094.f2:fis wheat-common [ Triticum aestivum ] Hap2a-like transcription wre1n.pk0143.h2:fis wheat-common factor [ Triticum aestivum ] Hap2b transcription factor wds1f.pk002.p21:fis wheat-common [ Triticum aestivum ] Hap2c transcription factor contig of: wheat-common wdi1c.pk002.b10 [ Triticum aestivum ] wr1.pk0153.c7:fis Hap2c-like transcription wre1n.pk0066.e4:fis wheat-common factor [ Triticum aestivum ] Hap2c-like transcription ncs.pk0013.c4:fis catalpa [ Catalpa factor speciosa ] Hap2c-like transcription p0117.chc1n94r:fis maize [ Zea mays ] factor Hap2c-like transcription rdi2c.pk011.f19:fis rice [ Oryza sativa ] factor Hap2c-like transcription sfl1.pk0101.g7:fis soybean [ Glycine factor max ] Hap2c-like transcription wdi1c.pk002.b10:fis wheat-common factor [ Triticum aestivum ] Hap5c-like transcription ect1c.pk001.k17:fis Canna [ Canna edulis ] factor Hap5a-like transcription vrr1c.pk004.o20:fis grape [Vitis sp.] factor Hap5a-like transcription clm1f.pk001.k17:fis maize [ Zea mays ] factor Hap5b-like transcription cde1n.pk003.a5:fis maize [ Zea mays ] factor Hap5b-like transcription cen3n.pk0164.a10:fis maize [ Zea mays ] factor Hap5b-like transcription p0118.chsbc77r maize [ Zea mays ] factor Hap5c-like transcription cco1n.pk055.o18:fis maize [ Zea mays ] factor Hap5c-like transcription cho1c.pk001.l23:fis maize [ Zea mays ] factor Hap5c-like transcription cse1c.pk001.h6:fis maize [ Zea mays ] factor Hap5a-like transcription rlm3n.pk005.d20:fis rice [ Oryza sativa ] factor Hap5b-like transcription rr1.pk0003.a3:fis rice [ Oryza sativa ] factor Hap5b-like transcription rr1.pk0039.d4:fis rice [ Oryza sativa ] factor Hap5c-like transcription rca1n.pk021.b20:fis rice [ Oryza sativa ] factor Hap5a-like transcription sdp2c.pk029k17:fis soybean [ Glycine factor max ] Hap5a-like transcription sdp2c.pk044.e5:fis soybean [ Glycine factor max ] Hap5b-like transcription sgs4c.pk004.j2 soybean [ Glycine factor max ] Hap5b-like transcription src3c.pk002.h4:fis soybean [ Glycine factor max ] Hap5b-like transcription src3c.pk009.b15:fis soybean [ Glycine factor max ] Hap5b-like transcription src3c.pk019.d4:fis soybean [ Glycine factor max ] Hap5c-like transcription sls1c.pk032.j4:fis soybean [ Glycine factor max ] Hap5 transcription factor wdk2c.pk009.e4:fis wheat-common [ Triticum aestivum ] Hap5a-like transcription contig of: wheat-common factor w1m96.pk036.j11 [ Triticum aestivum ] w1m96.pk060.d5:fis Hap5c-like transcription wle1n.pk0076.h7:fis wheat-common factor [ Triticum aestivum ] Hap5c-like transcription sgs4c.pk004.j2:fis soybean [ Glycine factor max ] Lec1-embryonic type eas1c.pk003.e16 amaranth [ Amaranthus retroflexus ] Lec1-embryonic type fds1n.pk008.m14 balsam pear [ Momordica charantia ] Lec1-embryonic type p0015.cdpgp75rb:fis maize [ Zea mays ] Lec1-embryonic type p0083.clder12r:fis maize [ Zea mays ] Lec1-embryonic type pps1c.pk002.l19 prickly poppy [ Argemone mexicana ] Lec1-embryonic type Contig of: soybean [ Glycine scb1c.pk004.j10 max ] se1.pk0042.d8:fis Lec1-embryonic type se2.11d12:fis soybean [ Glycine max ] Lec1-embryonic type ses2w.pk0015.a4:fis soybean [ Glycine max ] Lec1-embryonic type vs1n.pk013.m13:fis vernonia [ Vernonia mespilifolia ] Lec1-embryonic type wdk3c.pk023.h15:fis wheat-common [ Triticum aestivum ] Lec1-related CCAAT binding ect1c.pk007.p18:fis Canna [ Canna edulis ] protein Lec1-related CCAAT binding fds.pk0003.h5:fis balsam pear protein [ Momordica charantia ] Lec1-related CCAAT binding eef1c.pk004.c8:fis eucalyptus protein [ Eucalyptus grandis ] Lec1-related CCAAT binding cbn10.pk0005.e6:fis maize [ Zea mays ] protein Lec1-related CCAAT binding p0006.cbysa51r:fis maize [ Zea mays ] protein Lec1-related CCAAT binding rl0n.pk0061.c8:fis rice [ Oryza sativa ] protein Lec1-related CCAAT binding rsl1n.pk002.g10:fis rice [ Oryza sativa ] protein Lec1-related CCAAT binding ses4d.pk0037.e3:fis soybean [ Glycine protein max ] Lec1-related CCAAT binding src2c.pk003.i13:fis soybean [ Glycine protein max ] Lec1-related CCAAT binding src2c.pk011.m12:fis soybean [ Glycine protein max ] Lec1-related CCAAT binding src2c.pk025.b3:fis soybean [ Glycine protein max ] Lec1-related CCAAT binding src3c.pk028.j21:fis soybean [ Glycine protein max ] Lec1-related CCAAT binding wkm1c.pk0002.d7:fis wheat-common protein [ Triticum aestivum ] Lec1-related CCAAT binding wlk8.pk0001.e10:fis wheat-common protein [ Triticum aestivum ] Lec1-related CCAAT binding w1m96.pk037.k9:fis wheat-common protein [ Triticum aestivum ]

[0059] The Sequence Listing contains the one letter code for nucleotide sequence characters and the three letter codes for amino acids as defined in conformity with the IUPAC-IUBMB standards described in Nucleic Acids Res. 13:3021-3030 (1985) and in the Biochemical J. 219 (No. 2):345-373 (1984) which are herein incorporated by reference. The symbols and format used for nucleotide and amino acid sequence data comply with the rules set forth in 37 C.F.R. §1.822.
DETAILED DESCRIPTION OF THE INVENTION
[0060] All patents, patent applications and publications which are referred to herein are incorporated by reference in their entirety.
[0061] As used herein, an “isolated nucleic acid fragment” is a polymer of RNA or DNA that is single- or double-stranded, optionally containing synthetic, non-natural or altered nucleotide bases. An isolated nucleic acid fragment in the form of a polymer of DNA may be comprised of one or more segments of cDNA, genomic DNA or synthetic DNA. Nucleotides (usually found in their 5′-monophosphate form) are referred to by their single letter designation as follows: “A” for adenylate or deoxyadenylate (for RNA or DNA, respectively), “C” for cytidylate or deoxycytidylate, “G” for guanylate or deoxyguanylate, “U” for uridylate, “T” for deoxythymidylate, “R” for purines (A or G), “Y” for pyrimidines (C or T), “K” for g or T, “H” for A or C or T, “I” for inosine, and “N” for any nucleotide.
[0062] The terms “subfragment that is functionally equivalent” and “functionally equivalent subfragment” are used interchangeably herein. These terms refer to a portion or subsequence of an isolated nucleic acid fragment in which the ability to alter gene expression or produce a certain phenotype is retained whether or not the fragment or subfragment encodes an active enzyme. For example, the fragment or subfragment can be used in the design of recombinant DNA constructs to produce the desired phenotype in a transformed plant. Recombinant DNA constructs can be designed for use in co-suppression or antisense by linking a nucleic acid fragment or subfragment thereof, whether or not it encodes an active enzyme, in the appropriate orientation relative to a plant promoter sequence.
[0063] The terms “homology”, “homologous”, “substantially similar” and “corresponding substantially” are used interchangeably herein. They refer to nucleic acid fragments wherein changes in one or more nucleotide bases does not affect the ability of the nucleic acid fragment to mediate gene expression or produce a certain phenotype. These terms also refer to modifications of the nucleic acid fragments of the instant invention such as deletion or insertion of one or more nucleotides that do not substantially alter the functional properties of the resulting nucleic acid fragment relative to the initial, unmodified fragment. It is therefore understood, as those skilled in the art will appreciate, that the invention encompasses more than the specific exemplary sequences.
[0064] Moreover, the skilled artisan recognizes that substantially similar nucleic acid sequences encompassed by this invention are also defined by their ability to hybridize, under moderately stringent conditions (for example, 0.5×SSC, 0.1% SDS, 60° C.) with the sequences exemplified herein, or to any portion of the nucleotide sequences reported herein and which are functionally equivalent to the promoter of the invention. Stringency conditions can be adjusted to screen for moderately similar fragments, such as homologous sequences from distantly related organisms, to highly similar fragments, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes determine stringency conditions. One set of preferred conditions involves a series of washes starting with 6×SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2×SSC, 0.5% SDS at 45° C. for 30 min, and then repeated twice with 0.2×SSC, 0.5% SDS at 50° C. for 30 min. A more preferred set of stringent conditions involves the use of higher temperatures in which the washes are identical to those above except for the temperature of the final two 30 min washes in 0.2×SSC, 0.5% SDS was increased to 60° C. Another preferred set of highly stringent conditions involves the use of two final washes in 0.1×SSC, 0.1% SDS at 65° C.
[0065] With respect to the degree of substantial similarity between the target (endogenous) mRNA and the RNA region in the construct having homology to the target mRNA, such sequences should be at least 25 nucleotides in length, preferably at least 50 nucleotides in length, more preferably at least 100 nucleotides in length, again more preferably at least 200 nucleotides in length, and most preferably at least 300 nucleotides in length; and should be at least 80% identical, preferably at least 85% identical, more preferably at least 90% identical, and most preferably at least 95% identical.
[0066] Sequence alignments and percent similarity calculations may be determined using a variety of comparison methods designed to detect homologous sequences including, but not limited to, the Megalign program of the LASARGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences are performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments and calculation of percent identity of protein sequences using the Clustal method are KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. For nucleic acids these parameters are KTUPLE=2, GAP PENALTY=5, WINDOW=4 and DIAGONALS SAVED=4.
[0067] A “substantial portion” of an amino acid or nucleotide sequence comprises an amino acid or a nucleotide sequence that is sufficient to afford putative identification of the protein or gene that the amino acid or nucleotide sequence comprises. Amino acid and nucleotide sequences can be evaluated either manually by one skilled in the art, or by using computer-based sequence comparison and identification tools that employ algorithms such as BLAST (Basic Local Alignment Search Tool; Altschul et al (1993) J. Mol. Biol. 215:403-410; see also www.ncbi.nlm.nih.gov/BLAST/). In general, a sequence of ten or more contiguous amino acids or thirty or more contiguous nucleotides is necessary in order to putatively identify a polypeptide or nucleic acid sequence as homologous to a known protein or gene. Moreover, with respect to nucleotide sequences, gene-specific oligonucleotide probes comprising 30 or more contiguous nucleotides may be used in sequence-dependent methods of gene identification (e.g., Southern hybridization) and isolation (e.g., in situ hybridization of bacterial colonies or bacteriophage plaques). In addition, short oligonucleotides of 12 or more nucleotides may be used as amplification primers in PCR in order to obtain a particular nucleic acid fragment comprising the primers. Accordingly, a “substantial portion” of a nucleotide sequence comprises a nucleotide sequence that will afford specific identification and/or isolation of a nucleic acid fragment comprising the sequence. The instant specification teaches amino acid and nucleotide sequences encoding polypeptides that comprise one or more particular plant proteins. The skilled artisan, having the benefit of the sequences as reported herein, may now use all or a substantial portion of the disclosed sequences for purposes known to those skilled in this art. Accordingly, the instant invention comprises the complete sequences as reported in the accompanying Sequence Listing, as well as substantial portions of those sequences as defined above.
[0068] “Codon degeneracy” refers to divergence in the genetic code permitting variation of the nucleotide sequence without effecting the amino acid sequence of an encoded polypeptide. Accordingly, the instant invention relates to any nucleic acid fragment comprising a nucleotide sequence that encodes all or a substantial portion of the amino acid sequences set forth herein. The skilled artisan is well aware of the “codon-bias” exhibited by a specific host cell in usage of nucleotide codons to specify a given amino acid. Therefore, when synthesizing a nucleic acid fragment for improved expression in a host cell, it is desirable to design the nucleic acid fragment such that its frequency of codon usage approaches the frequency of preferred codon usage of the host cell
[0069] “Synthetic nucleic acid fragments” can be assembled from oligonucleotide building blocks that are chemically synthesized using procedures known to those skilled in the art. These building blocks are ligated and annealed to form larger nucleic acid fragments which may then be enzymatically assembled to construct the entire desired nucleic acid fragment. “Chemically synthesized”, as related to a nucleic acid fragment, means that the component nucleotides were assembled in vitro. Manual chemical synthesis of nucleic acid fragments may be accomplished using well established procedures, or automated chemical synthesis can be performed using one of a number of commercially available machines. Accordingly, the nucleic acid fragments can be tailored for optimal gene expression based on optimization of the nucleotide sequence to reflect the codon bias of the host cell. The skilled artisan appreciates the likelihood of successful gene expression if codon usage is biased towards those codons favored by the host. Determination of preferred codons can be based on a survey of genes derived from the host cell where sequence information is available.
[0070] “Gene” refers to a nucleic acid fragment that expresses a specific protein, including regulatory sequences preceding (5′ non-coding sequences) and following (3′ non-coding sequences) the coding sequence. “Native gene” refers to a gene as found in nature with its own regulatory sequences. The term “recombinant DNA construct” and “recombinant DNA construct” are used interchangeably herein. A recombinant DNA construct comprises an artificial combination of nucleic acid fragments, e.g., regulatory and coding sequences that are not found together in nature. For example, a recombinant DNA construct may comprise regulatory sequences and coding sequences that are derived from different sources, or regulatory sequences and coding sequences derived from the same source, but arranged in a manner different than that found in nature. A “foreign” gene refers to a gene not normally found in the host organism, but that is introduced into the host organism by gene transfer. Foreign genes can comprise native genes inserted into a non-native organism, or recombinant DNA constructs. A “transgene” is a gene that has been introduced into the genome by a transformation procedure.
[0071] “Coding sequence” refers to a DNA sequence that codes for a specific amino acid sequence. “Regulatory sequences” refer to nucleotide sequences located upstream (5′ non-coding sequences), within, or downstream (3′ non-coding sequences) of a coding sequence, and which influence the transcription, RNA processing or stability, or translation of the associated coding sequence. Regulatory sequences may include, but are not limited to, promoters, translation leader sequences, introns, and polyadenylation recognition sequences.
[0072] “Promoter” refers to a DNA sequence capable of controlling the expression of a coding sequence or functional RNA. The promoter sequence consists of proximal and more distal upstream elements, the latter elements often referred to as enhancers. Accordingly, an “enhancer” is a DNA sequence which can stimulate promoter activity and may be an innate element of the promoter or a heterologous element inserted to enhance the level or tissue-specificity of a promoter. Promoters may be derived in their entirety from a native gene, or be composed of different elements derived from different promoters found in nature, or even comprise synthetic DNA segments. It is understood by those skilled in the art that different promoters may direct the expression of a gene in different tissues or cell types, or at different stages of development, or in response to different environmental conditions. Promoters which cause a gene to be expressed in most cell types at most times are commonly referred to as “constitutive promoters”. New promoters of various types useful in plant cells are constantly being discovered; numerous examples may be found in the compilation by Okamuro and Goldberg, (1989) Biochemistry of Plants 15:1-82. It is further recognized that since in most cases the exact boundaries of regulatory sequences have not been completely defined, DNA fragments of some variation may have identical promoter activity.
[0073] An “intron” is an intervening sequence in a gene that does not encode a portion of the protein sequence. Thus, such sequences are transcribed into RNA but are then excised and are not translated. The term is also used for the excised RNA sequences. An “exon” is a portion of the sequence of a gene that is transcribed and is found in the mature messenger RNA derived from the gene, but is not necessarily a part of the sequence that encodes the final gene product.
[0074] The “translation leader sequence” refers to a DNA sequence located between the promoter sequence of a gene and the coding sequence. The translation leader sequence is present in the fully processed mRNA upstream of the translation start sequence. The translation leader sequence may affect processing of the primary transcript to mRNA, mRNA stability or translation efficiency. Examples of translation leader sequences have been described (Turner, R. and Foster, G. D. (1995) Molecular Biotechnology 3:225).
[0075] The “3′ non-coding sequences” refer to DNA sequences located downstream of a coding sequence and include polyadenylation recognition sequences and other sequences encoding regulatory signals capable of affecting mRNA processing or gene expression. The polyadenylation signal is usually characterized by affecting the addition of polyadenylic acid tracts to the 3′ end of the mRNA precursor. The use of different 3′ non-coding sequences is exemplified by Ingelbrecht et al, (1989) Plant Cell 1:671-680.
[0076] “RNA transcript” refers to the product resulting from RNA polymerase-catalyzed transcription of a DNA sequence. When the RNA transcript is a perfect complementary copy of the DNA sequence, it is referred to as the primary transcript or it may be a RNA sequence derived from post-transcriptional processing of the primary transcript and is referred to as the mature RNA. “Messenger RNA (mRNA)” refers to the RNA that is without introns and that can be translated into protein by the cell. “cDNA” refers to a DNA that is complementary to and synthesized from a mRNA template using the enzyme reverse transcriptase. The cDNA can be single-stranded or converted into the double-stranded form using the Klenow fragment of DNA polymerase I. “Sense” RNA refers to RNA transcript that includes the mRNA and can be translated into protein within a cell or in vitro. “Antisense RNA” refers to an RNA transcript that is complementary to all or part of a target primary transcript or mRNA and that blocks the expression of a target gene (U.S. Pat. No. 5,107,065). The complementarity of an antisense RNA may be with any part of the specific gene transcript, i.e., at the 5′ non-coding sequence, 3′ non-coding sequence, introns, or the coding sequence. “Functional RNA” refers to antisense RNA, ribozyme RNA, or other RNA that may not be translated but yet has an effect on cellular processes. The terms “complement” and “reverse complement” are used interchangeably herein with respect to mRNA transcripts, and are meant to define the antisense RNA of the message.
[0077] The term “endogenous RNA” refers to any RNA which is encoded by any nucleic acid sequence present in the genome of the host prior to transformation with the recombinant construct of the present invention, whether naturally-occurring or non-naturally occurring, i.e., introduced by recombinant means, mutagenesis, etc.
[0078] The term “non-naturally occurring” means artificial, not consistent with what is normally found in nature.
[0079] The term “operably linked” refers to the association of nucleic acid sequences on a single nucleic acid fragment so that the function of one is regulated by the other. For example, a promoter is operably linked with a coding sequence when it is capable of regulating the expression of that coding sequence (i.e., that the coding sequence is under the transcriptional control of the promoter). Coding sequences can be operably linked to regulatory sequences in a sense or antisense orientation. In another example, the complementary RNA regions of the invention can be operably linked, either directly or indirectly, 5′ to the target mRNA, or 3′ to the target mRNA, or within the target mRNA, or a first complementary region is 5′ and its complement is 3′ to the target mRNA.
[0080] The term “expression”, as used herein, refers to the production of a functional end-product. Expression of a gene involves transcription of the gene and translation of the mRNA into a precursor or mature protein. “Antisense inhibition” refers to the production of antisense RNA transcripts capable of suppressing the expression of the target protein. “Co-suppression” refers to the production of sense RNA transcripts capable of suppressing the expression of identical or substantially similar foreign or endogenous genes (U.S. Pat. No. 5,231,020).
[0081] “Mature” protein refers to a post-translationally processed polypeptide; i.e., one from which any pre- or propeptides present in the primary translation product have been removed. “Precursor” protein refers to the primary product of translation of mRNA; i.e., with pre- and propeptides still present. Pre- and propeptides may be but are not limited to intracellular localization signals.
[0082] “Stable transformation” refers to the transfer of a nucleic acid fragment into a genome of a host organism, including both nuclear and organellar genomes, resulting in genetically stable inheritance. In contrast, “transient transformation” refers to the transfer of a nucleic acid fragment into the nucleus, or DNA-containing organelle, of a host organism resulting in gene expression without integration or stable inheritance. Host organisms containing the transformed nucleic acid fragments are referred to as “transgenic” organisms. The preferred method of cell transformation of rice, corn and other monocots is the use of particle-accelerated or “gene gun” transformation technology (Klein et al, (1987) Nature (London) 327:70-73; U.S. Pat. No. 4,945,050), or an Agrobacterium-mediated method using an appropriate Ti plasmid containing the transgene (Ishida Y. et al, 1996, Nature Biotech. 14:745-750). The term “transformation” as used herein refers to both stable transformation and transient transformation.
[0083] Standard recombinant DNA and molecular cloning techniques used herein are well known in the art and are described more fully in Sambrook, J., Fritsch, E. F. and Maniatis, T. Molecular Cloning: A Laboratory Manual; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, 1989 (hereinafter “Sambrook”).
[0084] The term “recombinant” means, for example, that a nucleic acid sequence is made by an artificial combination of two otherwise separated segments of sequence, e.g., by chemical synthesis or by the manipulation of isolated nucleic acids by genetic engineering techniques. A “recombinant DNA construct” comprises an isolated polynucleotide operably linked to at least one regulatory sequence. The term also embraces an isolated polynucleotide comprising a region encoding all or part of a functional RNA and at least one of the naturally occurring regulatory sequences directing expression in the source (e.g., organism) from which the polynucleotide was isolated, such as, but not limited to, an isolated polynucleotide comprising a nucleotide sequence encoding a herbicide resistant target gene and the corresponding promoter and 3′ end sequences directing expression in the source from which sequences were isolated.
[0085] A “transgene” is a recombinant DNA construct that has been introduced into the genome by a transformation procedure.
[0086] As used herein, “contig” refers to a nucleotide sequence that is assembled from two or more constituent nucleotide sequences that share common or overlapping regions of sequence homology. For example, the nucleotide sequences of two or more nucleic acid fragments can be compared and aligned in order to identify common or overlapping sequences. Where common or overlapping sequences exist between two or more nucleic acid fragments, the sequences (and thus their corresponding nucleic acid fragments) can be assembled into a single contiguous nucleotide sequence.
[0087] “PCR” or “Polymerase Chain Reaction” is a technique for the synthesis of large quantities of specific DNA segments, consists of a series of repetitive cycles (Perkin Elmer Cetus Instruments, Norwalk, Conn.). Typically, the double stranded DNA is heat denatured, the two primers complementary to the 3′ boundaries of the target segment are annealed at low temperature and then extended at an intermediate temperature. One set of these three consecutive steps is referred to as a cycle.
[0088] The terms “recombinant construct”, “expression construct”, “recombinant expression construct”, “recombinant DNA construct” and “recombinant DNA construct” are used interchangeably herein. Such construct may be itself or may be used in conjunction with a vector. If a vector is used then the choice of vector is dependent upon the method that will be used to transform host plants as is well known to those skilled in the art. For example, a plasmid vector can be used. The skilled artisan is well aware of the genetic elements that must be present on the vector in order to successfully transform, select and propagate host cells comprising any of the isolated nucleic acid fragments of the invention. The skilled artisan will also recognize that different independent transformation events will result in different levels and patterns of expression (Jones et al, (1985) EMBO J. 4:2411-2418; De Almeida et al, (1989) Mol. Gen. Genetics 218:78-86), and thus that multiple events must be screened in order to obtain lines displaying the desired expression level and pattern. Such screening may be accomplished by Southern analysis of DNA, Northern analysis of mRNA expression, Western analysis of protein expression, or phenotypic analysis.
[0089] Co-suppression constructs in plants previously have been designed by focusing on overexpression of a nucleic acid sequence having homology to an endogenous mRNA, in the sense orientation, which results in the reduction of all RNA having homology to the overexpressed sequence (see Vaucheret et al (1998) Plant J 16:651-659; and Gura (2000) Nature 404:804-808). The overall efficiency of this phenomenon is low, and the extent of the RNA reduction is widely variable. Recent work has described the use of “hairpin” structures that incorporate all, or part, of an mRNA encoding sequence in a complementary orientation that results in a potential “stem-loop” structure for the expressed RNA (PCT Publication WO 99/53050 published on Oct. 21, 1999). This increases the frequency of co-suppression in the recovered transgenic plants. Another variation describes the use of plant viral sequences to direct the suppression, or “silencing”, of proximal mRNA encoding sequences (PCT Publication WO 98/36083 published on Aug. 20, 1998). Both of these co-suppressing phenomena have not been elucidated mechanistically, although recent genetic evidence has begun to unravel this complex situation (Elmayan et al (1998) Plant Cell 10:1747-1757).
[0090] Alternatively, a recombinant DNA construct designed to express antisense RNA for all or part of the instant nucleic acid fragment can be constructed by linking the gene or gene fragment in reverse orientation to plant promoter sequences. Either the co-suppression or antisense recombinant DNA constructs could be introduced into plants via transformation wherein expression of the corresponding endogenous genes are reduced or eliminated.
[0091] Molecular genetic solutions to the generation of plants with altered gene expression have a decided advantage over more traditional plant breeding approaches. Changes in plant phenotypes can be produced by specifically inhibiting expression of one or more genes by antisense inhibition or cosuppression (U.S. Pat. Nos. 5,190,931, 5,107,065 and 5,283,323). An antisense or cosuppression construct would act as a dominant negative regulator of gene activity. While conventional mutations can yield negative regulation of gene activity these effects are most likely recessive. The dominant negative regulation available with a transgenic approach may be advantageous from a breeding perspective. In addition, the ability to restrict the expression of a specific phenotype to the reproductive tissues of the plant by the use of tissue specific promoters may confer agronomic advantages relative to conventional mutations which may have an effect in all tissues in which a mutant gene is ordinarily expressed.
[0092] The person skilled in the art will know that special considerations are associated with the use of antisense or cosuppression technologies in order to reduce expression of particular genes. For example, the proper level of expression of sense or antisense genes may require the use of different recombinant DNA constructs utilizing different regulatory elements known to the skilled artisan. Once transgenic plants are obtained by one of the methods described above, it will be necessary to screen individual transgenics for those that most effectively display the desired phenotype. Accordingly, the skilled artisan will develop methods for screening large numbers of transformants. The nature of these screens will generally be chosen on practical grounds. For example, one can screen by looking for changes in gene expression by using antibodies specific for the protein encoded by the gene being suppressed, or one could establish assays that specifically measure enzyme activity. A preferred method will be one which allows large numbers of samples to be processed rapidly, since it will be expected that a large number of transformants will be negative for the desired phenotype.
[0093] Loss of function mutant phenotypes may be identified for the instant cDNA clones either by targeted gene disruption protocols or by identifying specific mutants for these genes contained in a maize population carrying mutations in all possible genes (Ballinger and Benzer (1989) Proc. Natl. Acad. Sci USA 86:9402-9406; Koes et al (1995) Proc. Natl. Acad. Sci USA 92:8149-8153; Bensen et al (1995) Plant Cell 7:75-84). The latter approach may be accomplished in two ways. First, short segments of the instant nucleic acid fragments may be used in polymerase chain reaction protocols in conjunction with a mutation tag sequence primer on DNAs prepared from a population of plants in which Mutator transposons or some other mutation-causing DNA element has been introduced (see Bensen, supra). The amplification of a specific DNA fragment with these primers indicates the insertion of the mutation tag element in or near the plant gene encoding the instant polypeptides. Alternatively, the instant nucleic acid fragment may be used as a hybridization probe against PCR amplification products generated from the mutation population using the mutation tag sequence primer in conjunction with an arbitrary genomic site primer, such as that for a restriction enzyme site-anchored synthetic adaptor. With either method, a plant containing a mutation in the endogenous gene encoding the instant polypeptides can be identified and obtained. This mutant plant can then be used to determine or confirm the natural function of the instant polypeptides disclosed herein.
[0094] The terms Hap3, Lec1, and Hap3/Lec1 are used interchangeably herein and refer to a class of transcription factors. The Hap3/Lec1 class is part of a broader family that includes other transcription factors such as Hap5, Hap2, and Lec1-CCAAT. The terms Hap3-like, Lec1-like, Hap3/Lec1-like, Hap5-like, Hap2-like, Lec1-CCAAT-like, etc. refer to any transcription factors that share sequence identity as disclosed herein and/or functionality with the nucleotide sequences and the corresponding amino acid sequences encoded by such nucleotide sequences disclosed in the present invention.
[0095] Surprisingly and unexpectedly, it has been found that there are a variety of regulatory/structural nucleic acid fragments, which heretofore have not been associated with altering oil phenotype in plants, that appear to be useful in altering oil phenotype in plants. In addition to the CCAAT-binding transcription factors, other proteins which heretofore have not been associated with altering oil phenotype in plants, have been identified. The nucleic acids identified encode a diverse class of regulatory and structural polypeptides whose expression correlates with altered oil phenotypes in plants. Altering the expression of these polypeptides would be expected to have an effect in altering oil accumulation in plants.
[0096] Other protein classes identified herein include:
[0097] a Hap2 transcription factor;
[0098] a Hap5 transcription factor;
[0099] a Hap3/Lec1 or Lec 1- CCAAT binding transcription factor.
[0100] They can be characterized as an isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
[0101] (a) a nucleic acid sequence encoding a fifth polypeptide having Hap2-like transcription factor activity, the fifth polypeptide having at least 70% identity based on the Clustal method of alignment when compared to a sixth polypeptide selected from the group consisting of SEQ ID NOs: 2, 4, 5, 6, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 46, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, 80, 82, or 208, 210, 212, 214, or 216; or
[0102] (b) a nucleic acid sequence encoding a seventh polypeptide having Hap5-like transcription factor activity, the seventh polypeptide having at least 80% identity based on the Clustal method of alignment when compared to an eighth polypeptide selected from the group consisting of SEQ ID NOs: 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 110, 112, 114, 116, 118, 120, 122, 124, 126, 128, or 221; or
[0103] (c) a nucleic acid sequence encoding a seventeenth polypeptide having Hap3/Lec1-like activity, the seventeenth polypeptide having at least 70% identity based on the Clustal method of alignment when compared to a eighteenth polypeptide selected from the group consisting of SEQ ID NOs: 130, 132, 134, or 136.
[0104] It is understood by one skilled in the art that other percent identity ranges may be useful in the above mentioned characterization. Useful percent identities would include, but not be limited to, 45%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% and all integer percentages from 45 to 100%.
[0105] The complement of the nucleotide fragments of this inventions are encompassed within the scope of this invention.
[0106] Those skilled in the art with also appreciate that the nucleotide fragment of this invention and/or the complement thereof can be used in whole or in part in antisense inhibition or co-suppression of a transformed plant.
[0107] In a more preferred embodiment, the first polypeptide mentioned above is as follows with respect to each part, the first polypeptide in
[0108] part (a) is a Hap2 transcription factor;
[0109] part (b) is a Hap5 transcription factor;
[0110] part (c) is a Hap3/Lec1 or Lec 1- CCAAT binding transcription factor
[0111] Lec1 homologs may be further identified by using conserved sequence motifs. The following amino acid sequence (given in single letter code, with “x” representing any amino acid). Under lined amino acids are those that are conserved in Lec1 but not found in Lec1-related proteins.
[0112] REQDxx M PxANVxRIMRxxLPxxAKIS D DAKEx I QECVSExISFxTxEA N x R Cxxxx RKTxxxE
[0113] In a further embodiment, this invention encompasses recombinant DNA construct comprising any of the isolated nucleic acid fragments of the invention or complement thereof operably linked to at least one regulatory sequence. It is also understood that recombinant DNA constructs comprising such fragments or complements thereof or parts of either can be used in antisense inhibition or suppression of a transformed plant.
[0114] Also within the scope of this invention is a plant comprising in its genome a recombinant DNA construct as described herein. Recombinant DNA constructs designed for plant expression such as those described herein can be introduced into a plant cell in a number of art-recognized ways. Those skilled in the art will appreciate that the choice of method might depend on the type of plant (i.e, monocot or dicot) and/or organelle (i.e., nucleus, chloroplast, mitochondria) targeted for transformation. Suitable methods for transforming plant cells include microinjection, electroporation, Agrobacterium mediated transformation, direct gene transfer and particle-accelerated or “gene gun” transformation technology as is discussed above.
[0115] Examples of plants which can be transformed include, but are not limited to, corn, soybean, wheat, rice, canola, Brassica, sorghum, sunflower, and coconut.
[0116] The regeneration, development and cultivation of plants from single plant protoplast transformants or from various transformed explants is well known in the art (Weissbach and Weissbach, In, Methods for Plant Molecular Biology, (Eds.), Academic Press, Inc., San Diego, Calif. (1988)). This regeneration and growth process typically includes the steps of selection of transformed cells, culturing those individualized cells through the usual stages of embryonic development through the rooted plantlet stage. Transgenic embryos and seeds are similarly regenerated. The resulting transgenic rooted shoots are thereafter planted in an appropriate plant growth medium such as soil.
[0117] The development or regeneration of plants containing the foreign, exogenous gene that encodes a protein of interest is well known in the art. Preferably, the regenerated plants are self-pollinated to provide homozygous transgenic plants. Otherwise, pollen obtained from the regenerated plants is crossed to seed-grown plants of agronomically important lines. Conversely, pollen from plants of these important lines is used to pollinate regenerated plants. A transgenic plant of the present invention containing a desired polypeptide is cultivated using methods well known to one skilled in the art.
[0118] There are a variety of methods for the regeneration of plants from plant tissue. The particular method of regeneration will depend on the starting plant tissue and the particular plant species to be regenerated. Methods for transforming dicots, primarily by use of Agrobacterium tumefaciens, and obtaining transgenic plants have been published for cotton (U.S. Pat. Nos. 5,004,863, 5,159,135, 5,518,908); soybean (U.S. Pat. Nos. 5,569,834 5,416,011, McCabe et. al., BiolTechnology 6:923 (1988), Christou et al., Plant Physiol. 87:671-674 (1988)); Brassica (U.S. Pat. No. 5,463,174); peanut (Cheng et al., Plant Cell Rep. 15:653-657 (1996), McKently et al., Plant Cell Rep. 14:699-703 (1995)); papaya; and pea (Grant et al., Plant Cell Rep. 15:254-258, (1995)).
[0119] Transformation of monocotyledons using electroporation, particle bombardment, and Agrobacterium have also been reported. Transformation and plant regeneration have been achieved in asparagus (Bytebier et al., Proc. Natl. Acad. Sci. (USA) 84:5354, (1987)); barley (Wan and Lemaux, Plant Physiol 104:37 (1994)); Zea mays (Rhodes et al., Science 240:204 (1988), Gordon-Kamm et al., Plant Cell 2:603-618 (1990), Fromm et al., BiolTechnology 8:833 (1990), Koziel et al., BiolTechnology 11: 194, (1993), Armstrong et al., Crop Science 35:550-557 (1995)); oat (Somers et al., BiolTechnology 10: 15 89 (1992)); orchard grass (Horn et al., Plant Cell Rep. 7:469 (1988)); rice (Toriyama et al., TheorAppl. Genet. 205:34, (1986); Part et al., Plant Mol. Biol. 32:1135-1148, (1996); Abedinia et al., Aust. J. Plant Physiol. 24:133-141 (1997); Zhang and Wu, Theor. Appl. Genet. 76:835 (1988); Zhang et al. Plant Cell Rep. 7:379, (1988); Battraw and Hall, Plant Sci. 86:191-202 (1992); Christou et al., Bio/Technology 9:957 (1991)); rye (De la Pena et al., Nature 325:274 (1987)); sugarcane (Bower and Birch, Plant J. 2:409 (1992)); tall fescue (Wang et al., BiolTechnology 10:691 (1992)), and wheat (Vasil et al., Bio/Technology 10:667 (1992); U.S. Pat. No. 5,631,152).
[0120] Assays for gene expression based on the transient expression of cloned nucleic acid constructs have been developed by introducing the nucleic acid molecules into plant cells by polyethylene glycol treatment, electroporation, or particle bombardment (Marcotte et al., Nature 335:454-457 (1988); Marcotte et al., Plant Cell 1:523-532 (1989); McCarty et al., Cell 66:895-905 (1991); Hattori et al., Genes Dev. 6:609-618 (1992); Goff et al., EMBO J. 9:2517-2522 (1990)).
[0121] Transient expression systems may be used to functionally dissect gene constructs (see generally, Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Press (1995)). It is understood that any of the nucleic acid molecules of the present invention can be introduced into a plant cell in a permanent or transient manner in combination with other genetic elements such as vectors, promoters, enhancers etc.
[0122] In addition to the above discussed procedures, practitioners are familiar with the standard resource materials which describe specific conditions and procedures for the construction, manipulation and isolation of macromolecules (e.g., DNA molecules, plasmids, etc.), generation of recombinant organisms and the screening and isolating of clones, (see for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press (1989); Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Press (1995); Birren et al., Genome Analysis: Detecting Genes, 1, Cold Spring Harbor, N.Y. (1998); Birren et al., Genome Analysis: Analyzing DNA, 2, Cold Spring Harbor, N.Y. (1998); Plant Molecular Biology: A Laboratory Manual, eds. Clark, Springer, N.Y. (1997)).
[0123] Seeds obtained from such plants and oil obtained from these seeds constitute another aspect of the present invention.
[0124] In an even further aspect, the invention concerns a method for altering oil phenotype in a plant which comprises:
[0125] (a) transforming a plant with a recombinant DNA construct of the invention;
[0126] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0127] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0128] In a more specific embodiment, the invention concerns a method for altering oil phenotype in a plant which comprises:
[0129] (a) transforming a plant with a recombinant DNA construct comprising isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
[0130] (i) a nucleic acid sequence encoding a plant Hap3/Lec1 transcription factor having at least 60% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of even SEQ ID NOs: from 130 to 148, and SEQ ID NOs: 195 and 196;
[0131] (ii) the complement of the nucleic acid sequence of (i);
[0132] (iii) the sequence of (i) or (ii) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
[0133] (iv) a nucleic acid sequence encoding a plant Lec -related CCAAT binding transcription factor having at least 60% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of even SEQ ID NOs: from 150 to 178, and SEQ ID NOs: 197 to 202;
[0134] (v) the complement of the nucleic acid sequence of (vii);
[0135] (vi) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
[0136] wherein said nucleic acid sequence is operably linked to at least one regulatory sequence;
[0137] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0138] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0139] It is understood by one skilled in the art that other percent identity ranges may be useful in the above mentioned method. Useful percent identities would include, but not be limited to, 45%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% and all integer percentages from 45 to 100%.
[0140] In an even further aspect, this invention concerns a method to isolate nucleic acid fragments associated with altering oil phenotype in a plant which comprises:
[0141] (a) comparing even SEQ ID NOs: from 2 to 178, and 206 to 214, and SEQ ID NOs: 179 to 202, 216 to 219, 221, and 222 with other polypeptide sequences fort he purpose of identifying polypeptides associated with altering oil phenotype in a plant;
[0142] (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);
[0143] (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b); and
[0144] (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering oil phenotype by sequence dependent protocols.
[0145] In a most preferred aspect, this invention concerns a method for altering oil phenotype in a plant which comprises:
[0146] (a) transforming a plant with a recombinant DNA construct comprising an isolated nucleic acid fragment operably linked to at least one regulatory sequence wherein said fragment has a nucleic acid sequence encoding a polypeptide having a sequence identity of at least 60% based on the Clustal method of alignment when compared to a polypeptide selected from the group consisting of even;
[0147] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0148] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0149] It is understood by one skilled in the art that other percent identity ranges may be useful in the above mentioned method. Useful percent identities would include, but not be limited to, 45%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95% and all integer percentages from 45 to 100%.
[0150] In another aspect, this invention also concerns a method of mapping genetic variations related to altered oil phenotypes in a plant comprising:
[0151] (a) crossing two plant varieties; and
[0152] (b) evaluating genetic variations with respect to nucleic acid sequences set forth in any one of the odd SEQ ID NOs: from 1 to 177, or 207 to 215, or SEQ ID NO: 220 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.
[0153] In another embodiment, this invention concerns a method of molecular breeding to obtain altered oil phenotypes in a plant comprising:
[0154] (a) crossing two plant varieties; and
[0155] (b) evaluating genetic variations with respect to nucleic acid sequences set forth in any one of the odd SEQ ID NOs: from 1 to 177, or 207 to 215, or SEQ ID NO: 220 in progeny plants resulting from the cross of step (a) wherein the evaluation is made using a method selected from the group consisting of: RFLP analysis, SNP analysis, and PCR-based analysis.
[0156] The genetic variability at a particular locus (gene) due to even minor base changes can alter the pattern of restriction enzyme digestion fragments that can be generated. Pathogenic alterations to the genotype can be due to deletions or insertions within the gene being analyzed or even single nucleotide substitutions that can create or delete a restriction enzyme recognition site. RFLP analysis takes advantage of this and utilizes Southern blotting with a probe corresponding to the gene of interest.
[0157] Thus, if a polymorphism (i.e., a commonly occurring variation in a gene or segment of DNA; also, the existence of several forms of a gene (alleles) in the same species) creates or destroys a restriction endonuclease cleavage site, or if it results in the loss or insertion of DNA (e.g., a variable nucleotide tandem repeat (VNTR) polymorphism), it will alter the size or profile of the DNA fragments that are generated by digestion with that restriction endonuclease. As such, individuals that possess a variant sequence can be distinguished from those having the original sequence by restriction fragment analysis. Polymorphisms that can be identified in this manner are termed “restriction fragment length polymorphisms: (“RFLPs”). RFLPs have been widely used in human and plant genetic analyses (Glassberg, UK Patent Application 2135774; Skolnick et al, Cytogen. Cell Genet. 32:58-67 (1982); Botstein et al, Ann. J. Hum. Genet. 32:314-331 (1980); Fischer et al (PCT Application WO 90/13668; Uhlen, PCT Appliction WO 90/11369).
[0158] A central attribute of “single nucleotide polymorphisms” or “SNPs” is that the site of the polymorphism is at a single nucleotide. SNPs have certain reported advantages over RFLPs or VNTRs. First, SNPs are more stable than other classes of polymorphisms. Their spontaneous mutation rate is approximately 10 −9 (Kornberg, DNA Replication, W. H. Freeman & Co., San Francisco, 1980), approximately, 1,000 times less frequent than VNTRs (U.S. Pat. No. 5,679,524). Second, SNPs occur at greater frequency, and with greater uniformity than RFLPs and VNTRs. As SNPs result from sequence variation, new polymorphisms can be identified by sequencing random genomic or cDNA molecules. SNPs can also result from deletions, point mutations and insertions. Any single base alteration, whatever the cause, can be a SNP. The greater frequency of SNPs means that they can be more readily identified than the other classes of polymorphisms.
[0159] SNPs can be characterized using any of a variety of methods. Such methods include the direct or indirect sequencing of the site, the use of restriction enzymes where the respective alleles of the site create or destroy a restriction site, the use of allele-specific hybridization probes, the use of antibodies that are specific for the proteins encoded by the different alleles of the polymorphism or by other biochemical interpretation. SNPs can be sequenced by a number of methods. Two basic methods may be sued for DNA sequencing, the chain termination method of Sanger et al, Proc. Natl. Acad. Sci. (U.S.A.) 74:5463-5467 (1977), and the chemical degradation method of Maxam and Gilbert, Proc. Natl.,Acad. Sci. (U.S.A.) 74: 560-564 (1977).
[0160] Polymerase chain reaction (“PCR”) is a powerful technique used to amplify DNA millions of fold, by repeated replication of a template, in a short period of time. (Mullis et al, Cold Spring Harbor Symp. Quant. Biol. 51:263-273 (1986); Erlich et al, European Patent Application 50,424; European Patent Application 84,796; European Patent Application 258,017, European Patent Application 237,362; Mullis, European Patent Application 201,184, Mullis et al U.S. Pat. No. 4,683,202; Erlich, U.S. Pat. No. 4,582,788; and Saiki et al, U.S. Pat. No. 4,683,194). The process utilizes sets of specific in vitro synthesized oligonucleotides to prime DNA synthesis. The design of the primers is dependent upon the sequences of DNA that are desired to be analyzed. The technique is carried out through many cycles (usually 20-50) of melting the template at high temperature, allowing the primers to anneal to complementary sequences within the template and then replicating the template with DNA polymerase.
[0161] The products of PCR reactions are analyzed by separation in agarose gels followed by ethidium bromide staining and visualization with UV transillumination. Alternatively, radioactive dNTPs can be added to the PCR in order to incorporate label into the products. In this case the products of PCR are visualized by exposure of the gel to x-ray film. The added advantage of radiolabeling PCR products is that the levels of individual amplification products can be quantitated.
[0162] Furthermore, single point mutations can be detected by modified PCR techniques such as the ligase chain reaction (“LCR”) and PCR-single strand conformational polymorphisms (“PCR-SSCP”) analysis. The PCR technique can also be sued to identify the level of expression of genes in extremely small samples of material, e.g., tissues or cells from a body. The technique is termed reverse transcription-PCR (“RT-PCR”).
[0163] In another embodiment, this invention concerns a method for altering oil phenotype in a plant which comprises:
[0164] (a) transforming a plant with a recombinant DNA construct comprising isolated nucleotide fragment comprising a nucleic acid sequence selected from the group consisting of:
[0165] (i) a nucleic acid sequence encoding a plant Hap3/Lec1 transcription factor having at least 70% identity based on the Clustal method of alignment when compared to a second polypeptide selected from the group consisting of SEQ ID NOs: 130 to 148, and SEQ ID NOs: 195 and 196;
[0166] (ii) the complement of the nucleic acid sequence of (iv);
[0167] (iii) the sequence of (iv) or (v) or a part thereof which is useful in antisense inhibition or co-suppression in a transformed plant;
[0168] (b) growing the transformed plant under conditions suitable for expression of the recombinant DNA construct; and
[0169] (c) selecting those transformed plants whose oil phenotype has been altered compared to the oil phenotype of an untransformed plant.
[0170] It is understood by one skilled in the art that other percent identity ranges may be useful in the above mentioned method. Useful percent identities would include, but not be limited to, 45%, 50%, 55%, 60%, 65%, 75%, 80%, 85%, 90%, 95% and all integer percentages from 45 to 100%.
[0171] In another aspect this invention concerns a method to isolate nucleic acid fragments associated with altering oil phenotype in a plant which comprises:
[0172] (a) comparing SEQ ID NOs: 130 to 148, and SEQ ID NOs: 195, 196, and 206 with other polypeptide sequences for the purpose of identifying polypeptides associated with altering oil phenotype in a plant;
[0173] (b) identifying the conserved sequences(s) or 4 or more amino acids obtained in step (a);
[0174] (c) making region-specific nucleotide probe(s) or oligomer(s) based on the conserved sequences identified in step (b); and
[0175] (d) using the nucleotide probe(s) or oligomer(s) of step (c) to isolate sequences associated with altering oil phenotype by sequence dependent protocols.
EXAMPLES
[0176] The present invention is further defined in the following Examples, in which parts and percentages are by weight and degrees are Celsius, unless otherwise stated. The disclosure of each reference set forth herein is incorporated herein by reference in its entirety.
Example 1
[0177] Composition of cDNA Libraries; Isolation and Sequencing of cDNA Clones
[0178] cDNA libraries representing mRNAs from various plant tissues were prepared. The characteristics of the libraries are described below.
TABLE 2 cDNA Libraries from Various Plants Library Tissue Clone cbn10 Corn Developing Kernel (Embryo and cbn10.pk0005.e6:fis Endosperm); 10 Days After Pollination cbn10.pk0064.e6 cc71se-a Corn Callus Type II Tissue, Somatic Embryo cc71se-a.pk0002.e11:fis Formed cc71se-b Corn Callus Type II Tissue, Somatic Embryo cc71se-b.pk0018.e4:fis Formed cca Corn Callus Type II Tissue, Undifferentiated, cca.pk0026.d6 Highly Transformable ccase-b Corn Callus Type II Tissue, Somatic Embryo ccase-b.pk0003.b9:fis Formed, Highly Transformable cco1n.pk062.j7 cco1n.pk086.d20:fis cco1n Corn Cob of 67 Day Old Plants Grown in cco1n.pk0014.d4:fis Green House* cco1n.pk055.o18 cco1n.pk089.g17 cco1n.pk068.f18:fis cde1c Corn ( Zea Mays , B73) developing embryo cde1c.pk003.o22:fis 20 DAP ced1n Corn ( Zea mays , B73) developing embryo cde1n.pk003.a5 20 DAP normalized cde1n.pk001.n24:fis cdo1c Corn ( Zea mays L.) ovary, 5 days after cdolc.pk001.c1:fis silking (includes pedicel and glumes) ceb3 Corn Embryo 20 Days After Pollination ceb3.pk0012.a7 ceb5 Corn Embryo 30 Days After Pollination ceb5.pk0081.b4 cen3n.pk0164.a10 cen3n Corn Endosperm 20 Days After Pollination* cen3n.pk0044.b8:fis cen3n.pk0112.e10:fis cho1c.pk003.p17:fis cho1c.pk003.n23 cho1c Corn ( Zea mays L., Alexho Synthetic High Oil) cho1c.pk004.b19:f15 embryo 20 DAP cho1c.pk007.l21:fis cho1c.pk001.l23:fis cho1c.pk009.g10 clm1f Corn ( Zea mays , B73) leaf at V6-VT (full length) clm1f.pk001.k17 clm1f.pk002.o13:fis cpd1c Corn ( Zea mays L.) pooled BMS treated with cpd1c.pk011.15:fis chemicals related to protein kinases cpd1c.pk008.e21 cpf1c Corn ( Zea mays L.) pooled BMS treated with cpf1c.pk006.e3:fis chemicals related to protein synthesis cpj1c Corn ( Zea mays L.) pooled BMS treated with cpj1c.pk005.m20:fis chemicals related to membrane ionic force cr1n Corn Root From 7 Day Old Seedlings* cr1n.pk0080.g6 cse1c Corn ( Zea mays L.) seedling at V2 stage treated cse1c.pk001.h6 with Ethylene collected at 6 hr, 23 hr, 72 hr cta1n Corn Tassel* cta1n.pk0070.f3:fis cta1n.pk0074.h11 ctn1c Corn ( Zea mays L., B73) night harvested tassel ctn1c.pk002.o4 ect1c Canna edulis Tubers ect1c.pk001.k17:fis ect1c.pk007.p18:fis eef1c Eucalyptus tereticornis flower buds from adult eef1c.pk004.c8:fis tree etr1c Cattail ( Typha latifolia ) root etr1c.pk006.f9 fds Momordica charantia Developing Seed fds.pk0003.h5:fis hss1c Scierotinia infected sunflower plants hss1c.pk011.h10:fis ncs Catalpa speciosa Developing Seed ncs.pk0013.c4 p0006 Young shoot p0006.cbysa51r:fis p0015 13 DAP embryo p0015.cdpgu90r:fis p0015.cdpfm55r:fis p0016 Tassel shoTassel shoots, pooled, 0.1-1.4 cm p0016.ctsbf56rb p0026 Regenerating callus 5 days after auxin removal p0026.ccrab39r Hi-II callus 223a, 1129e p0027 GS3 shoot cultures that were transformed with p0027.cgsag51r PHP5869 and were maintained on 273T shoot multiplication medium since 3/17/94 (sample received on 5/29/96 for RNA prep). The original transformation was done on 11/6/93 p0031 CM45 shoot culture. It was initiated on 2/28/96 p0031.ccmau15r:fis from seed derived meristems. The culture was p0031.ccmbc81r maintained on 273N medium. p0032 Regenerating callus, 10 and 14 days after auxin p0032.crcac77r:fis removal. Hi-II callus 223a, 1129e 10 days. Hi-II callus 223a, 1129e 14 days p0037 corn Root Worm infested V5 roots p0037.crwbs90r:fis p0083.cldct11r:fis p0083 7 DAP whole kernels p0083.cldeu68r:fis p0083.clder12r p0086 P0067 screened 1; 11 DAP pericarp p0086.cbsaa24r p0118 Night harvested, pooled stem tissue from the p0118.chsbc77r 4-5 internodes subtending the tassel; V8-V12 p0118.chsbh89r stages, Screened 1 p0125 Anther: Prophase I sceened 1 p0125.czaab60rb:f15 p0126 Night harvested leaf tissue; V8-V10 p0126.cnlau71r:fis p0134 Hi-II callus 223a, 1129e, 10 days hi-II callus p0134.carah47r 233a, 1129e, 14 days pps1c Prickly poppy developing seeds pps1c.pk001.h3:fis pps1c.pk007.j21:fis rbm5c Rice ( Oryza sativa , Cypress) bran 10 days after rbm5c.pk001.a19 milling rca1c Rice Nipponbare Callus. rca1c.pk007.b22:fis rca1n.pk029.n22 rca1n.pk002.j3 rca1n Rice ( Oryza sativa L., Nipponbare) callus rca1n.pk021.b20:fis normalized. rca1n.pk004.j14:fis rca1n.pk026.m9 rca1n.pk008.o5:fis r10n.pk096.h23 r10n.pk0061.c8:fis r10n Rice 15 Day Old Leaf* r10n.pk131.j17 r10n.pk0015.a4:fis rlm3n Rice ( Oryza Sativa , YM) leaf mixture (rsr9) rlm3n.pk005.d20:fis normalized at 45 C. for 24 hrs using 20 fold excess of driver rlr2 Rice ( Oryza sativa L.) leaf (15 DAG) 2 hrs after rlr2.pk0012.d2 infection of strain 4360-R-62 (AVR2-YAMO); Resistant rlr24 Rice Leaf 15 Days After Germination, 24 Hours rlr24.pk0032.e10 After Infection of Strain Magnaporthe grisea 4360-R-62 (AVR2-YAMO); Resistant rls6 Rice Leaf 15 Days After Germination, 6 Hours rls6.pk0033.a9:fis After Infection of Strain Magnaporthe grisea 4360-R-67 (AVR2-YAMO); Susceptible rls72 Rice Leaf 15 Days After Germination, 72 Hours rls72.pk0023.c8:fis After Infection of Strain Magnaporthe grisea 4360-R-67 (AVR2-YAMO); Susceptible rr1 Rice Root of Two Week Old Developing rr1.pk0039.d4:fis rr1.pk0003.a3:fis rr1.pk097.f22:fis rr1.pk0047.g12:fis rsl1n.pk002.g10:fis rsl1n.pk002.j2:fis rsl1n Rice ( Oryza sativa , YM) 15 day old rsl1n.pk006.n24:fis normalized rsl1n.pk013.g2 scb1c Soybean ( Glycine max L., 2872) Embryogenic scb1c.pk004.n19:fis suspension culture subjected to 4 bombardments and collected 12 hrs later. sde4c Soybean Developing Embryo (9-11 mm) sde4c.pk0001.a2:fis sdp2c.pk003.o5:fis sdp2c Soybean ( Glycine max L.) developing pods sdp2c.pk023.n6:fis 6-7 mm sdp2c.pk029.k17:fis sdp2c.pk044.e5:fis sdp3c.pk018.b9:fis sdp3c Soybean Developing Pods (8-9 mm) sdp3c.pk019.n1:fis spd4c Soybean ( Glycine max L.) developing pods sdp4c.pk009.e3s 10-12 mm dp4c.pk016.e10 sdr1f Soybean ( Glycine max , Wye) 10 day old root sdr1f.pk001.p7 sds1f Soybean ( Glycine max , Wye) 11 day old sds1f.pk001.f7:fis seedling full length library using trehalose se1 Soybean Embryo, 6 to 10 Days After Flowering se1.pk0042.d8:fis se2 Soybean Embryo, 13 Days After Flowering se2.11d12:fis ses2w Soybean Embryogenic Suspension 2 Weeks ses2w.pk0015.a4:fis After Subculture ses2w.pk0035.a9:fis ses2w.pk0012.d10:fis ses4d.pk0037.e3:fis ses4d Soybean Embryogenic Suspension 4 Days After ses4d.pk0044.c12 Subculture ses4d.pk0006.a12 ses4d.pk0006.a12:fis ses4d.pk0043.d10:fis sfl1.pk0102.h8 sf11.pk131.j19 sfl1 Soybean Immature Flower sfl1.pk135.g3 sfl1.pk0029.h10:fis sgc5c Soybean ( Glycine max L., Wye) germanating sgc5c.pk001.h16 cotyledon (¾ yellow; 15-24 DAG) sgs1c Soybean Seeds 4 Hours After Germination sgs1c.pk004.f19:fis sgs4c Soybean ( Glycine max L.) seeds 2 days after sgs4c.pk004.j2 germination. sgs4c.pk006.g6 sgs4c.pk006.n21 sic1c Soybean ( Glycine max ) pooled tissue of root, sic1c.pk003.o13:fis stem, and leaf with iron chlorosis conditions sic1c.pk003.o18:fis sif1c Soybean ( Glycine max ) pooled tissue of basal sif1c.pk001.m16:fis stem and root infected with fusarium sls1c Soybean ( Glycine max L., S1990) infected with sls1c.pk010.l1:fis Sclerotinia sclerotiorum mycelium . sls1c.pk032.j4 sls1c Soybean ( Glycine max L., S1990) infected with sls1c.pk010.l1:fis Sclerotinia sclerotiorum mycelium . sls1c.pk020.h24 sls2c Soybean ( Glycine max L., Manta) infected with sls2c.pk007.c23:fis Sclerotinia sclerotiorum mycelium . sr1 Soybean Root sr1.pk0041.a11:fis sr1.pk0049.c2 srb Scarlett runner bean (R. Goldberg) srb.08g04 src1c Soybean 8 Day Old Root Infected With Cyst src1c.pk003.o16:fis Nematode src2c.pk025.b3:fis src2c Soybean ( Glycine max L., 437654) 8 day old src2c.pk011.m12:fis root inoculated with eggs of cyst Nematode src2c.pk009.g9:fis (Race 1) for 4 days. src2c.pk003.i13:fis src3c.pk018.d10:fis sr3c.pk011.g22 src3c Soybean 8 Day Old Root Infected With Cyst src3c.pk012.n16:fis Nematode src3c.pk019.d4:fis src3c.pk009.b15 src3c.pk028.j21:fis srr1c Soybean 8-Day-Old Root srr1c.pk001.i24:fis srr3c Soybean 8-Day-Old Root srr3c.pk001.l10:fis tlw1c Tobacco ( Nicotiana benthamiana ) Leaves tlw1c.pk006.o16 Wounded by Abrasion and Harvested After 1.5 Hour. vdb1c Grape (Vitis sp.) developing bud vdb1c.pk001.m5:fis vmb1na Grape (Vitis sp.) midstage berries normalized vmb1na.pk015.d18:fis vpl1c Grape (Vitis sp.) In vitro plantlets vpl1c.pk008.o5:fis vrr1c Grape (Vitis sp.) resistant roots vrr1c.pk004.o20:fis vs1n Vernonia Seed* vs1n.pk013.m13:fis wdelf Wheat ( Triticum aestivum , Hi Line) developing wde1f.pk003.h2:fis endosperm 2-7 DPA wdk2c Wheat Developing Kernel, 7 Days After Anthesis. wdk2c.pk009.e4 wdk2c Wheat Developing Kernel, 7 Days After Anthesis. wdk2c.pk018.c16:fis wdk3c Wheat Developing Kernel, 14 Days After wdk3c.pk023.h15:fis Anthesis. wdk5c Wheat Developing Kernel, 30 Days After wdk5c.pk006.m13 Anthesis wdk9n Wheat ( Triticum aestivu , Spring Wheat) kernels wdk9n.pk001.k5 3, 7, 14 and 21 days after anthesis wdr1f Wheat ( Triticum aestivum ) developing root (full wdr1f.pk003.b21:fis length) wds1f Wheat developing seedling full length wds1f.pk002.p21:fis wia1c Wheat ( Triticum aestivum , Hi Line) immature wia1c.pk001.d20:fis anthers wkm1c Wheat Kernel malted 55 Hours at 22 Degrees wkm1c.pk0002.d7:fis Celsius wl1n Wheat Leaf From 7 Day Old Seedling* wl1n.pk0114.f9 wle1n Wheat Leaf From 7 Day Old Etiolated Seedling* wle1n.pk0076.h7:fis wlk8 Wheat Seedlings 8 Hours After Treatment With wlk8.pk0001.e10:fis Fungicide** wlm96.pk060.d5 wlm96.pk037.k9:fis wlm96 Wheat Seedlings 96 Hours After Inoculation With wlm96.pk035.j11:fis Erysiphe graminis f. sp tritici wlm96.pk0007.e4:fis wr1 Wheat Root From 7 Day Old Seedling wr1.pk0094.f2:fis wr1.pk0153.c7:fis wr1.pk148.f7:fis wre1n Wheat Root From 7 Day Old Etiolated wre1n.pk0066.e4:fis Seedling* wre1n.pk0143.h2:fis

[0179] cDNA libraries may be prepared by any one of many methods available. For example, the cDNAs may be introduced into plasmid vectors by first preparing the cDNA libraries in Uni-ZAP™ XR vectors according to the manufacturer's protocol (Stratagene Cloning Systems, La Jolla, Calif.). The Uni-ZAP™ XR libraries are converted into plasmid libraries according to the protocol provided by Stratagene. Upon conversion, cDNA inserts will be contained in the plasmid vector pBluescript. In addition, the cDNAs may be introduced directly into precut Bluescript II SK(+) vectors (Stratagene) using T4 DNA ligase (New England Biolabs), followed by transfection into DH10B cells according to the manufacturer's protocol (GIBCO BRL Products). Once the cDNA inserts are in plasmid vectors, plasmid DNAs are prepared from randomly picked bacterial colonies containing recombinant pBluescript plasmids, or the insert cDNA sequences are amplified via polymerase chain reaction using primers specific for vector sequences flanking the inserted cDNA sequences. Amplified insert DNAs or plasmid DNAs are sequenced in dye-primer sequencing reactions to generate partial cDNA sequences (expressed sequence tags or “ESTs”; see Adams et al, (1991) Science 252:1651-1656). The resulting ESTs are analyzed using a Perkin Elmer Model 377 fluorescent sequencer.
[0180] Full-insert sequence (FIS) data is generated utilizing a modified transposition protocol. Clones identified for FIS are recovered from archived glycerol stocks as single colonies, and plasmid DNAs are isolated via alkaline lysis. Isolated DNA templates are reacted with vector primed M13 forward and reverse oligonucleotides in a PCR-based sequencing reaction and loaded onto automated sequencers. Confirmation of clone identification is performed by sequence alignment to the original EST sequence from which the FIS request is made.
[0181] Confirmed templates are transposed via the Primer Island transposition kit (PE Applied Biosystems, Foster City, Calif.) which is based upon the Saccharomyces cerevisiae Ty1 transposable element (Devine and Boeke (1994) Nucleic Acids Res. 22:3765-3772). The in vitro transposition system places unique binding sites randomly throughout a population of large DNA molecules. The transposed DNA is then used to transform DH10B electro-competent cells (Gibco BRL/Life Technologies, Rockville, Md.) via electroporation. The transposable element contains an additional selectable marker (named DHFR; Fling and Richards (1983) Nucleic Acids Res. 11:5147-5158), allowing for dual selection on agar plates of only those subclones containing the integrated transposon. Multiple subclones are randomly selected from each transposition reaction, plasmid DNAs are prepared via alkaline lysis, and templates are sequenced (ABI Prism dye-terminator ReadyReaction mix) outward from the transposition event site, utilizing unique primers specific to the binding sites within the transposon.
[0182] Sequence data is collected (ABI Prism Collections) and assembled using Phred/Phrap (P. Green, University of Washington, Seattle). Phrep/Phrap is a public domain software program which re-reads the ABI sequence data, re-calls the bases, assigns quality values, and writes the base calls and quality values into editable output files. The Phrap sequence assembly program uses these quality values to increase the accuracy of the assembled sequence contigs. Assemblies are viewed by the Consed sequence editor (D. Gordon, University of Washington, Seattle).
[0183] In some of the clones the cDNA fragment corresponds to a portion of the 3′-terminus of the gene and does not cover the entire open reading frame. In order to obtain the upstream information one of two different protocols are used. The first of these methods results in the production of a fragment of DNA containing a portion of the desired gene sequence while the second method results in the production of a fragment containing the entire open reading frame. Both of these methods use two rounds of PCR amplification to obtain fragments from one or more libraries. The libraries some times are chosen based on previous knowledge that the specific gene should be found in a certain tissue and some times are randomly-chosen. Reactions to obtain the same gene may be performed on several libraries in parallel or on a pool of libraries. Library pools are normally prepared using from 3 to 5 different libraries and normalized to a uniform dilution. In the first round of amplification both methods use a vector-specific (forward) primer corresponding to a portion of the vector located at the 5′-terminus of the clone coupled with a gene-specific (reverse) primer. The first method uses a sequence that is complementary to a portion of the already known gene sequence while the second method uses a gene-specific primer complementary to a portion of the 3′-untranslated region (also referred to as UTR). In the second round of amplification a nested set of primers is used for both methods. The resulting DNA fragment is ligated into a pBluescript vector using a commercial kit and following the manufacturer's protocol. This kit is selected from many available from several vendors including Invitrogen (Carlsbad, Calif.), Promega Biotech (Madison, Wis.), and Gibco-BRL (Gaithersburg, Md.). The plasmid DNA is isolated by alkaline lysis method and submitted for sequencing and assembly using Phred/Phrap, as above.
Example 2
[0184] Identification of cDNA Clones
[0185] cDNA clones encoding proteins involved in altering plant oil traits were identified by gene profiling (see Example 7) and by conducting BLAST (Basic Local Alignment Search Tool; Altschul et al (1993) J. Mol. Biol. 215:403-410; see also www.ncbi.nlm.nih.gov/BLAST/) searches for similarity to sequences contained in the BLAST “nr” database (comprising all non-redundant GenBank CDS translations, sequences derived from the 3-dimensional structure Brookhaven Protein Data Bank, the last major release of the SWISS-PROT protein sequence database, EMBL, and DDBJ databases). The cDNA sequences obtained in Example 1 were analyzed for similarity to all publicly available DNA sequences contained in the “nr” database using the BLASTN algorithm provided by the National Center for Biotechnology Information (NCBI). The DNA sequences were translated in all reading frames and compared for similarity to all publicly available protein sequences contained in the “nr” database using the BLASTX algorithm (Gish and States (1993) Nat. Genet. 3:266-272) provided by the NCBI. For convenience, the P-value (probability) of observing a match of a cDNA sequence to a sequence contained in the searched databases merely by chance as calculated by BLAST are reported herein as “pLog” values, which represent the negative of the logarithm of the reported P-value. Accordingly, the greater the pLog value, the greater the likelihood that the cDNA sequence and the BLAST “hit” represent homologous proteins.
[0186] ESTs submitted for analysis are compared to the genbank database as described above. ESTs that contain sequences more 5- or 3-prime can be found by using the BLASTn algorithm (Altschul et al (1997) Nucleic Acids Res. 25:3389-3402.) against the DuPont proprietary database comparing nucleotide sequences that share common or overlapping regions of sequence homology. Where common or overlapping sequences exist between two or more nucleic acid fragments, the sequences can be assembled into a single contiguous nucleotide sequence, thus extending the original fragment in either the 5 or 3 prime direction. Once the most 5-prime EST is identified, its complete sequence can be determined by Full Insert Sequencing as described in Example 1. Homologous genes belonging to different species can be found by comparing the amino acid sequence of a known gene (from either a proprietary source or a public database) against an EST database using the tBLASTn algorithm. The tBLASTn algorithm searches an amino acid query against a nucleotide database that is translated in all 6 reading frames. This search allows for differences in nucleotide codon usage between different species, and for codon degeneracy.
Example 3
[0187] Characterization of cDNA Clones Encoding Proteins Involved in Altering Oil
Phenotypes
[0188] The BLASTX search using the EST sequences from clones listed in Table 3 revealed similarity of the polypeptides encoded by the cDNAs to Hap2 homologs, Hap5 homologs, and Lec1 transcription factors from various species including Arabidopsis thaliana, rice ( Oryza sativa ), corn ( Zea mays ), soybean ( Glycine max ), cucmber ( Cucumis sativus ), Sordaria ( Sordaria macrospora ), sesame ( Sesamum indicum ), grape (Vitis sp.), Brassica ( Brassica napus ), and tobacco ( Nicotiana tabacum ). Shown in Table 3 are the BLAST results for individual ESTs (“EST”), the sequences of the entire cDNA inserts comprising the indicated cDNA clones (“FIS”), the sequences of contigs assembled from two or more ESTs (“Contig”), sequences of contigs assembled from an FIS and one or more ESTs (“Contig*”), or sequences encoding an entire protein derived from an FIS, a contig, or an FIS and PCR (“CGS”):
TABLE 3 BLAST Results for Sequences Encoding Polypeptides Homologous to Proteins Involved in Altering Oil Phenotypes SEQ ID NO. Gene Name Clone Homolog Genbank # pLOG 2 Hap2a ncs.pk0013.c4 No hits — 4 Hap2c etr1c.pk006.f9 No hits — 6 Hap2a vmb1na.pk015.d18 Arabidopsis 11282597 8.1 8 Hap2a vpl1c.pk008.o5:fis Grape 7141243 91.2 10 Hap2c vdb1c.pk001.m5:fis Rice 7489565 38.0 12 Hap2c cho1c.pk004.b19:fis Rice 7489565 94.3 14 Hap2c p0015.cdpgu90r:fis Rice 7489565 96.2 16 Hap2a cta1n.pk0070.f3:fis Rice 7489565 38.1 18 Hap2a cco1n.pk0014.d4:fis Arabidopsis 6634774 37.2 20 Hap2a cco1n.pk086.d20:fis Arabidopsis 6634774 36.3 22 Hap2b p0126.cnlau71r:fis Rice 7489565 23.7 24 Hap2b p0104.cabav52r Rice 7489565 16.7 26 Hap2b cho1c.pk007.l21:fis Rice 7489565 35.0 contig of: cca.pk0026.d6 28 Hap2c cen3n.pk0061.e10:fis Rice 7489565 43.5 cen3n.pk0135.c2 cho1c.pk001.n24 p0092.chwae40r 30 Hap2c cpf1c.pk006.e3:fis Rice 7489565 44.0 contig of: Rice 7489565 32 Hap2c cr1n.pk0080.g6 35.0 p0003.cgpge51r 34 Hap2c p0015.cdpfm55r:fis Arabidopsis 4587559 26.4 36 Hap2 p0083.cldct11r:fis Rice 7489565 91.4 38 Hap2 p0083.cldeu68r:fis Rice 7489565 14.2 40 Hap2a pps1c.pk001.h3:fis Arabidopsis 9293997 45.5 42 Hap2c pps1c.pk007.j21:fis Arabidopsis 5903072 53.7 44 Hap2 rr1.pk0030.f7:fis Rice 7489565 identical 46 Hap2a r1s72.pk0023.c8:fis Arabidopsis 9293997 36.5 48 Hap2a rca1n.pk002.c15 Grape 7141243 7.7 50 Hap2a rds3c.pk001.g9 Rice 7489565 18.2 52 Hap2b rca1n.pk002.j3:fis Rice 7489565 26.0 54 Hap2c rca1n.pk029.n22:fis Arabidopsis 8778470 29.2 56 Hap2b r10n.pk131.j17 Rice 7489565 10.5 58 Hap2a sdp3c.pk018.b9:fiS Arabidopsis 2398521 74.5 60 Hap2a sfl1.pk0102.h8 Grape 7141243 36.7 62 Hap2a srr3c.pk001.l10:fis Brassica 1586551 48.7 64 Hap2a sdp2c.pk003.o5:fiS Arabidopsis 6634774 53.0 66 Hap2b sif1c.pk001.m16:fis Arabidopsis 6714441 180.0 68 Hap2c src1c.pk003.o16:fis Rice 7489565 33.5 70 Hap2c src3c.pk012.m6:fis Rice 7489565 31.5 72 Hap2a hss1c.pk011.h10:fis Arabidopsis 9293997 48.7 74 Hap2c wr1.pk0094.f2:fis Rice 7489565 92.7 76 Hap2a wre1n.pk0143.h2:fis Arabidopsis 6634774 35.0 78 Hap2b wds1f.pk002.p21:fis Arabidopsis 6714441 26.5 contig of: 80 Hap2b wdi1c.pk002.b10 Rice 7489565 38.5 wr1.pk0153.c7:fis 82 Hap2c wre1n.pk0066.e4:fis Rice 7489565 42.7 84 Hap5c ect1c.pk001.k17:fis Rice 5257260 57.0 86 Hap5a vrr1c.pk004.o20:fis Arabidopsis 6523090 93.0 88 Hap5a clm1f.pk001.k17:fis Arabidopsis 6523090 66.7 90 Hap5b cde1n.pk003.a5:fis Arabidopsis 3776575 57.0 92 Hap5b cen3n.pk0164.a10:fis Arabidopsis 3776575 57.0 94 Hapsb p0118.chsbc77r Arabidopsis 3776575 58.5 96 Hap5c cco1n.pk055.o18 Rice 5257260 41.0 98 Hap5c cho1c.pk001.l23:fis Rice 5257260 82.0 100 Hap5c cse1c.pk001.h6:fis Rice 5257260 86.4 102 Hap5a rlm3n.pk005.d20:fis Arabidopsis 6523090 66.7 104 Hap5b rr1.pk0003.a3:fis Arabidopsis 6289057 58.5 106 Hap5b rr1.pk0039.d4:fis Arabidopsis 3776575 57.2 108 Hap5c rca1n.pk021.b20:fis Rice 5257260 74.0 110 Hap5a sdp2c.pk029.k17:fis Arabidopsis 6523090 90.5 112 Hap5a sdp2c.pk044.e5:fis Arabidopsis 6523090 92.4 114 Hap5b sgs4c.pk004.j2 Arabidopsis 3776575 18.5 116 Hap5b src3c.pk002.h4:fis Arabidopsis 6289057 61.1 118 Hap5b src3c.pk009.b15:fis Arabidopsis 6289057 61.5 120 Hap5b src3c.pk019.d4:fis Arabidopsis 6056368 51.5 122 Hap5c sls1c.pk032.j4:fis Arabidopsis 6289057 74.5 124 Hap5 wdk2c.pk009.e4:fis Rice 5257260 20.0 Contig of: 126 Hap5a w1m96.pk036.j11 Arabidopsis 9758288 19.7 w1m96.pk060.d5:fis 128 Hap5c wle1n.pk0076.h7:fis Rice 5257260 82.0 130 Lec1 eas1c.pk003.e16 Arabidopsis 9758795 49.2 132 Lec1 fds1n.pk008.m14 Arabidopsis 9758795 46.1 134 Lec1 p0015.cdpg75rb:fis Arabidopsis 9758795 45.4 136 Lec1 p0083.clder12r:fis Arabidopsis 6552738 35.2 138 Lec1 pps1c.pk002.l19 Arabidopsis 9758795 45.2 Contig of: 140 Lec1 scb1c.pk004.j10 Arabidopsis 9758795 47.4 se1.pk0042.d8:fis 142 Lec1 se2.11d12:fis Arabidopsis 9758795 52.2 144 Lec1 ses2w.pk0015.a4:fis Arabidopsis 9758795 43.7 146 Lec1 vs1n.pk013.m13:fis Arabidopsis 9758795 53.1 148 Lec1 wdk3c.pk023.h15:fis Arabidopsis 9758795 36.7 150 Lec1-CCAAT ect1c.pk007.p18:fis Zea mays 22380 44.7 152 Lec1-CCAAT fds.pk0003.h5:fis Arabidopsis 6729485 57.7 154 Lec1-CCAAT eef1c.pk004.c8:fis Zea mays 22380 61.7 156 Lec1-CCAAT cbn10.pk0005.e6:fis Zea mays 22380 72.2 158 Lec1-CCAAT p0006.cbysa51r:fis Arabidopsis 2244810 55.5 160 Lec1-CCAAT rl0n.pk0061.c8:fis Zea mays 22380 46.5 162 Lec1-CCAAT rsl1n.pk002.g10:fis Zea mays 22380 68.7 164 Lec1-CCAAT ses4d.pk0037.e3:fis Arabidopsis 2398529 49.0 166 Lec1-CCAAT src2c.pk003.i13:fis Arabidopsis 3738293 41.1 168 Lec1-CCAAT src2c.pk011.m12:fis Arabidopsis 6729485 62.0 170 Lec1-CCAAT src2c.pk025.b3:fis Zea mays 22380 45.5 172 LecI-CCAAT src3c.pk028.j21:fis Zea mays 22380 54.3 174 Lec1-CCAAT wkm1c.pk0002.d7:fis Zea mays 22380 79.5 176 Lec1-CCAAT wlk8.pk0001.e10:fis Arabidopsis 2398529 52.7 178 LecI-CCAAT wlm96.pk037.k9:fis Zea mays 22380 73.5 206 Lec1 rice genome seq Oryza sativa 7378310 180 208 Hap2 ncs.pk0013.c4:fis Arabidopsis 9293997 46.7 210 Hap2 p0117.chcln94r:fis Oryza sativa 1489565 26.0 212 Hap2 rdi2c.pk011.f19:fis Oryza sativa 1489565 45.0 214 Hap2 sfl1.pk0101.g7:fis Vitis sp. 7141243 38.4 216 Hap2 wdi1c.pk002.b10:fis Oryza sativa 1489565 40.3 221 Hap5 sgs4c.pk004.j2:fis Arabidopsis 15223482 69.0

[0189] The sequence of the entire cDNA insert in the clones listed in Table 3 was determined. Further sequencing and searching of the DuPont proprietary database allowed the identification of other corn, rice, soybean and/or wheat clones encoding polypetides involved in altering oil phenotypes. The BLASTX search using the sequences from clones listed in Table 4 revealed similarity of the polypeptides encoded by the various cDNAs from plant and fungal species (noted by their NCBI General Identifier No. in Tables 3 and 4). Shown in Table 4 are the BLAST results for individual ESTs (“EST”), the sequences of the entire cDNA inserts comprising the indicated cDNA clones (“FIS”), sequences of contigs assembled from two or more ESTs (“Contig”), sequences of contigs assembled from an FIS and one or more ESTs (“Contig*”), or sequences encoding the entire protein derived from an FIS, a contig, or an FIS and PCR (“CGS”):
TABLE 4 Percent Identity of Amino Acid Sequences Deduced From the Nucleotide Sequences of cDNA Clones Encoding Polypeptides Homologous to Polypeptides Involved in Altering Plant Oil Phenotypes SEQ ID NO. Accession No. (SEQ ID NO) Percent Identity 2 1586551 (187) 23.4% 4 7489565 (181) 27.4% 6 11282597 (179) 22.1% 10 7489565 (181) 36.1% 12 7489565 (181) 67.2% 14 7489565 (181) 70.6% 16 7489565 (181) 33.2% 18 6634774 (182) 40.1% 20 6634774 (182) 39.1% 22 7489565 (181) 28.2% 24 7489565 (181) 53.2% 26 7489565 (181) 34.0% 28 7489565 (181) 39.5% 30 7489565 (181) 39.5% 32 7489565 (181) 35.5% 34 4587559 (202) 54.1% 36 7489565 (181) 67.2% 38 7489565 (181) 29.0% 40 9293997 (217) 31.5% 42 5903072 (184) 35.3% 46 5903072 (184) 33.7% 48 7141243 (180) 34.5% 50 7489565 (181) 35.7% 52 7489565 (181) 27.2% 54 8778470 (185) 40.5% 56 7489565 (181) 22.1% 58 2398521 (186) 49.1% 60 7141243 (180) 40.9% 62 1586551 (187) 37.8% 64 6634774 (182) 49.2% 66 6714441 (188) 32.5% 68 7489565 (181) 32.4% 70 7489565 (181) 31.1% 72 9293997 (217) 40.6% 74 7489565 (181) 68.5% 76 6634774 (182) 36.5% 78 6714441 (188) 23.7% 80 7489565 (181) 34.5% 82 7489565 (181) 37.4% 84 5257260 (189) 62.9% 86 6523090 (190) 77.7% 88 6523090 (190) 53.8% 90 3776575 (191) 50.7% 92 3776575 (191) 51.6% 94 3776575 (191) 60.0% 96 5257260 (189) 62.7% 98 5257260 (189) 75.0% 100 5257260 (189) 77.5% 102 6523090 (190) 53.8% 104 6289057 (192) 60.6% 106 3776575 (191) 52.1% 108 5257260 (189) 77.9% 110 6523090 (190) 70.3% 112 6523090 (190) 70.7% 114 3776575 (191) 35.7% 116 6289057 (192) 53.2% 118 6289057 (192) 52.8% 120 6056368 (193) 73.0% 122 6289057 (192) 57.1% 124 5257260 (189) 27.3% 126 9758288 (194) 46.3% 128 5257260 (189) 74.9% 130 9758795 (196) 49.0% 132 9758795 (196) 49.7% 134 9758795 (196) 49.8% 136 6552738 (195) 38.9% 208 9293997 (217) 34.9% 210 7489565 (218) 28.6% 212 7489565 (218) 35.7% 214 7141243 (219) 42.3% 216 7489565 (218) 34.9% 221 15223482 (222) 64.8%

[0190] Sequence alignments and percent identity calculations were performed using the Megalign program of the LASERGENE bioinformatics computing suite (DNASTAR Inc., Madison, Wis.). Multiple alignment of the sequences was performed using the Clustal method of alignment (Higgins and Sharp (1989) CABIOS. 5:151-153) with the default parameters (GAP PENALTY=10, GAP LENGTH PENALTY=10). Default parameters for pairwise alignments using the Clustal method were KTUPLE 1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5. Sequence alignments and BLAST scores and probabilities indicate that the nucleic acid fragments comprising the instant cDNA clones encode a substantial portion of cDNAs to receptor protein kinases, MEK3 homologs, Hap2 homologs, LIP 15 homologs, calcium EF-hand proteins, ATP citrate lyase, glucose metabolism proteins such as SNF1 homologs, Lec1 transcription factors, and seed developmentally regulated transcription factors such as CKC (Aintegumenta-like) homologs.
Example 4
[0191] Expression of Recombinant DNA Constructs in Monocot Cells
[0192] A recombinant DNA construct comprising a cDNA encoding the instant polypeptides in sense orientation with respect to the maize 27 kD zein promoter that is located 5′ to the cDNA fragment, and the 10 kD zein 3′ end that is located 3′ to the cDNA fragment, can be constructed. The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites (Ncol or Smal) can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the digested vector pML103 as described below. Amplification is then performed in a standard PCR. The amplified DNA is then digested with restriction enzymes Ncol and SmaI and fractionated on an agarose gel. The appropriate band can be isolated from the gel and combined with a 4.9 kb NcoI-SmaI fragment of the plasmid pML103. Plasmid pML103 has been deposited under the terms of the Budapest Treaty at ATCC (American Type Culture Collection, 10801 University Blvd., Manassas, Va. 20110-2209), and bears accession number ATCC 97366. The DNA segment from pML103 contains a 1.05 kb SaII-NcoI promoter fragment of the maize 27 kD zein gene and a 0.96 kb SmaI-SaII fragment from the 3′ end of the maize 10 kD zein gene in the vector pGem9Zf(+) (Promega). Vector and insert DNA can be ligated at 15° C. overnight, essentially as described (Maniatis). The ligated DNA may then be used to transform E. coli XL 1-Blue (Epicurian Coli XL-1 Blue™; Stratagene). Bacterial transformants can be screened by restriction enzyme digestion of plasmid DNA and limited nucleotide sequence analysis using the dideoxy chain termination method (Sequenase™ DNA Sequencing Kit; U.S. Biochemical). The resulting plasmid construct would comprise a recombinant DNA construct encoding, in the 5′ to 3′ direction, the maize 27 kD zein promoter, a cDNA fragment encoding the instant polypeptides, and the 10 kD zein 3′ region.
[0193] The recombinant DNA construct described above can then be introduced into corn cells by the following procedure. Immature corn embryos can be dissected from developing caryopses derived from crosses of the inbred corn lines H99 and LH132. The embryos are isolated 10 to 11 days after pollination when they are 1.0 to 1.5 mm long. The embryos are then placed with the axis-side facing down and in contact with agarose-solidified N6 medium (Chu et al (1975) Sci. Sin. Peking 18:659-668). The embryos are kept in the dark at 27° C. Friable embryogenic callus consisting of undifferentiated masses of cells with somatic proembryoids and embryoids borne on suspensor structures proliferates from the scutellum of these immature embryos. The embryogenic callus isolated from the primary explant can be cultured on N6 medium and sub-cultured on this medium every 2 to 3 weeks.
[0194] The plasmid, p35S/Ac (obtained from Dr. Peter Eckes, Hoechst Ag, Frankfurt, Germany) may be used in transformation experiments in order to provide for a selectable marker. This plasmid contains the Pat gene (see European Patent Publication 0 242 236) which encodes phosphinothricin acetyl transferase (PAT). The enzyme PAT confers resistance to herbicidal glutamine synthetase inhibitors such as phosphinothricin. The pat gene in p35S/Ac is under the control of the 35S promoter from Cauliflower Mosaic Virus (Odell et al (1985) Nature 313:810-812) and the 3′ region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens.
[0195] The particle bombardment method (Klein et al (1987) Nature 327:70-73) may be used to transfer genes to the callus culture cells. According to this method, gold particles (1 μm in diameter) are coated with DNA using the following technique. Ten μg of plasmid DNAs are added to 50 μL of a suspension of gold particles (60 mg per ml). Calcium chloride (50 μL of a 2.5 M solution) and spermidine free base (20 μL of a 1.0 M solution) are added to the particles. The suspension is vortexed during the addition of these solutions. After 10 minutes, the tubes are briefly centrifuged (5 sec at 15,000 rpm) and the supernatant removed. The particles are resuspended in 200 μL of absolute ethanol, centrifuged again and the supernatant removed. The ethanol rinse is performed again and the particles resuspended in a final volume of 30 μL of ethanol. An aliquot (5 μL) of the DNA-coated gold particles can be placed in the center of a Kapton™ flying disc (Bio-Rad Labs). The particles are then accelerated into the corn tissue with a Biolistic™ PDS-1000/He (Bio-Rad Instruments, Hercules Calif.), using a helium pressure of 1000 psi, a gap distance of 0.5 cm and a flying distance of 1.0 cm.
[0196] For bombardment, the embryogenic tissue is placed on filter paper over agarose-solidified N6 medium. The tissue is arranged as a thin lawn and covered a circular area of about 5 cm in diameter. The petri dish containing the tissue can be placed in the chamber of the PDS-1000/He approximately 8 cm from the stopping screen. The air in the chamber is then evacuated to a vacuum of 28 inches of Hg. The macrocarrier is accelerated with a helium shock wave using a rupture membrane that bursts when the He pressure in the shock tube reaches 1000 psi.
[0197] Seven days after bombardment the tissue can be transferred to N6 medium that contains bialophos (5 mg per liter) and lacks casein or proline. The tissue continues to grow slowly on this medium. After an additional 2 weeks the tissue can be transferred to fresh N6 medium containing bialophos. After 6 weeks, areas of about 1 cm in diameter of actively growing callus can be identified on some of the plates containing the bialophos-supplemented medium. These calli may continue to grow when sub-cultured on the selective medium.
[0198] Plants can be regenerated from the transgenic callus by first transferring clusters of tissue to N6 medium supplemented with 0.2 mg per liter of 2,4-D. After two weeks the tissue can be transferred to regeneration medium (Fromm et al (1990) Bio/Technology 8:833-839).
Example 5
[0199] Expression of Recombinant DNA Constructs in Dicot Cells
[0200] A seed-specific expression cassette composed of the promoter and transcription terminator from the gene encoding the β subunit of the seed storage protein phaseolin from the bean Phaseolus vulgaris (Doyle et al (1986) J. Biol. Chem. 261:9228-9238) can be used for expression of the instant polypeptides in transformed soybean. The phaseolin cassette includes about 500 nucleotides upstream (5′) from the translation initiation codon and about 1650 nucleotides downstream (3′) from the translation stop codon of phaseolin. Between the 5′ and 3′ regions are the unique restriction endonuclease sites Nco I (which includes the ATG translation initiation codon), Sma I, Kpn I and Xba I. The entire cassette is flanked by Hind III sites.
[0201] The cDNA fragment of this gene may be generated by polymerase chain reaction (PCR) of the cDNA clone using appropriate oligonucleotide primers. Cloning sites can be incorporated into the oligonucleotides to provide proper orientation of the DNA fragment when inserted into the expression vector. Amplification is then performed as described above, and the isolated fragment is inserted into a pUC18 vector carrying the seed expression cassette.
[0202] Soybean embryos may then be transformed with the expression vector comprising sequences encoding the instant polypeptides. To induce somatic embryos, cotyledons, 3-5 mm in length dissected from surface sterilized, immature seeds of the soybean cultivar A2872, can be cultured in the light or dark at 26° C. on an appropriate agar medium for 6-10 weeks. Somatic embryos which produce secondary embryos are then excised and placed into a suitable liquid medium. After repeated selection for clusters of somatic embryos which multiplied as early, globular staged embryos, the suspensions are maintained as described below.
[0203] Soybean embryogenic suspension cultures can be maintained in 35 mL liquid media on a rotary shaker, 150 rpm, at 26° C. with florescent lights on a 16:8 hour day/night schedule. Cultures are subcultured every two weeks by inoculating approximately 35 mg of tissue into 35 mL of liquid medium.
[0204] Soybean embryogenic suspension cultures may then be transformed by the method of particle gun bombardment (Klein et al (1987) Nature (London) 327:70-73, U.S. Pat. No. 4,945,050). A DuPont Biolistic™ PDS1000/HE instrument (helium retrofit) can be used for these transformations.
[0205] A selectable marker gene which can be used to facilitate soybean transformation is a recombinant DNA construct composed of the 35S promoter from Cauliflower Mosaic Virus (Odell et al (1985) Nature 313:810-812), the hygromycin phosphotransferase gene from plasmid pJR225 (from E. coli; Gritz et al(1983) Gene 25:179-188) and the 3′ region of the nopaline synthase gene from the T-DNA of the Ti plasmid of Agrobacterium tumefaciens. The seed expression cassette comprising the phaseolin 5′ region, the fragment encoding the instant polypeptides and the phaseolin 3′ region can be isolated as a restriction fragment. This fragment can then be inserted into a unique restriction site of the vector carrying the marker gene.
[0206] To 50 μL of a 60 mg/mL 1 μm gold particle suspension is added (in order): 5 μL DNA (1 μg/μL), 20 μL spermidine (0.1 M), and 50 μL CaCl 2 (2.5 M). The particle preparation is then agitated for three minutes, spun in a microfuge for 10 seconds and the supernatant removed. The DNA-coated particles are then washed once in 400 μL 70% ethanol and resuspended in 40 μL of anhydrous ethanol. The DNA/particle suspension can be sonicated three times for one second each. Five μL of the DNA-coated gold particles are then loaded on each macro carrier disk.
[0207] Approximately 300-400 mg of a two-week-old suspension culture is placed in an empty 60×15 mm petri dish and the residual liquid removed from the tissue with a pipette. For each transformation experiment, approximately 5-10 plates of tissue are normally bombarded. Membrane rupture pressure is set at 1100 psi and the chamber is evacuated to a vacuum of 28 inches mercury. The tissue is placed approximately 3.5 inches away from the retaining screen and bombarded three times. Following bombardment, the tissue can be divided in half and placed back into liquid and cultured as described above.
[0208] Five to seven days post bombardment, the liquid media may be exchanged with fresh media, and eleven to twelve days post bombardment with fresh media containing 50 mg/mL hygromycin. This selective media can be refreshed weekly. Seven to eight weeks post bombardment, green, transformed tissue may be observed growing from untransformed, necrotic embryogenic clusters. Isolated green tissue is removed and inoculated into individual flasks to generate new, clonally propagated, transformed embryogenic suspension cultures. Each new line may be treated as an independent transformation event. These suspensions can then be subcultured and maintained as clusters of immature embryos or regenerated into whole plants by maturation and germination of individual somatic embryos.
Example 6
[0209] Expression Vector for Plant Transformation by Particle Gun Bombardment.
[0210] A seed specific gene expression cassette was used for making recombinant DNA constructs for expression of candidate genes in corn. The expression cassette is composed of the 0.9 kb oleosin promoter, the intron 1 of the maize shrunken 1 gene and adjacent exon (Vasil et al, 1989, Plant Physiol 91: 1575-1579; Mascarenhas et al, 1990, Plant Mol Biol 15:913-920) and 3′ transcription termination region from the nopaline synthase (Nos) gene. In between the exon adjacent to the shrunken 1 gene and the nopaline synthase (Nos) gene are unique restriction endonuclease sites MfeI and XmaI. This vector has been designated pBN256 (REF. Jennie Shen's patent). pMUT256 refers to a pBN256 plasmid in which a EcoRI site has been removed by site directed mutagenesis. A modified version of pMUT256, designated pMUT256e was modified by additon of a synthetic multiple cloning site. The synthetic polylinker was generated by annealing of oligos (5′-acagtacagtacagtacagtacagt-3′) and (5′-actgtactgtactgtacgtgactgt-3′) [SEQ ID NOs: 430 and 431, respectively] and subsequent subcloning into the pMut256 open with MfeI and XmaI. Additional expression cassettes/vectors will be described in reference to specific examples where they have been used (see below).
Example 7
[0211] Isolation and Cloning of Candidate Genes into Embryo-specific Plant Expression Vectors.
[0212] HAP3/LEC1 (Heme-Activated Protein 3/Leafy Cotyledon 1):
[0213] A full length clone (p0015.cdpgp75rb, SEQ ID NOs: 263) for the corn homolog of the HAP3/Lec1 gene was obtained from Dupont/Pioneer EST Database. The ORF of maize HAP3/Lec1 (a 1 kb SaII/HpaI fragment, PCT Application No. WO 00/28058, published on May 18, 2000) was moved into an expression cassette containing a maize oleosin promoter (a 0.9 kb BamHI/XhoI fragment, PCT Application No. WO 99/64579, published on Dec. 16, 1999) and a polyadenylation sequence from the Agrobacterium nopaline synthase gene. This expression cassette was then subcloned adjacent to a 35S::Bar expression cassette (Sidorenko et al (2000) Plant J 22:471-482). The resulting expression cassettes flanked by T-DNA border sequences were then mobilized into the Agrobacterium “super-binary” vector (Komari, 1990) using electroporation. Additional constructs were made to confer expression patterns different from those obtained with the oleosin promoter. A ubiquitin promoter (UBI, Christensen et al (1992) Plant Mol Biol 18:675-680), a lipid transfer protein (LTP) promoter (U.S. Pat. No. 5,525,716), and a gamma zein promoter (GZP) (Boronat et al (1986) Plant Science 47: 95-102) were each fused to Lec1 as described above for the oleosin promoter. The two transcription units, LTP-Lec1 and GZP-Lec1, were combined into one expression construct next to the 35S:Bar expression construct and flanked by T-DNA border sequences (as described above).
[0214] HAP2 (Heme-Activated Protein 2):
[0215] A full length clone (cho1c.pk006.b14, a 30 nucleotide shorter cDNA than cho1c.pk004.b19:fis, shown in SEQ ID NO: 27) for the corn homolog of the HAP2 gene was obtained from Dupont/Pioneer EST Database. The ApoI/ApaI 1.1 kb fragment of cho1c.pk006.b14 was isolated and subcloned into pMUT256e opened by digestion with EcoRI/ApaI. One clone was selected for corn transformation by restriction digestion analysis for correct insert size. Subcloning artifacts were excluded by 5′ and 3′ sequence of the vector-insert boundaries.
[0216] HAP5 (Heme-Activated Protein 5):
[0217] A full length clone (cho1c.pk001.I23, shown in SEQ ID NO: 113) for the corn homolog of HAP5 gene was obtained from Dupont/Pioneer EST Database. The EcoRI/ApaI 1.1 kb fragment of cho1c.pk001.I23 was isolated and subcloned into pMUT256e opened by digestion with EcoRI/ApaI. One clone was selected for corn transformation after restriction digestion analysis for correct insert size. Subcloning artifacts were excluded by 5′ and 3′ sequence of the vector-insert boundaries.
Example 8
[0218] Transformation of Immature Embryos BY Particle Bombardment and Regeneration of Corn Plants
[0219] Immature maize embryos from greenhouse donor plants are bombarded with a plasmid containing the gene of the invention operably linked to a weak promoter, such as the nos promoter, or an inducible promoter, such as ln2, plus a plasmid containing the selectable marker gene PAT (Wohileben et al (1988) Gene 70:25-37) that confers resistance to the herbicide Bialaphos. Transformation is performed as follows. The ears are surface sterilized in 30% Chloral bleach plus 0.5% Micro detergent for 20 minutes, and rinsed two times with sterile water. The immature embryos are excised and placed embryo axis side down (scutellum side up), 25 embryos per plate. These are cultured on 560 L medium 4 days prior to bombardment in the dark. Medium 560 L is an N6-based medium containing Eriksson's vitamins, thiamine, sucrose, 2,4-D, and silver nitrate. The day of bombardment, the embryos are transferred to 560 Y medium for 4 hours and are arranged within the 2.5-cm target zone. Medium 560Y is a high osmoticum medium (560 L with high sucrose concentration). A plasmid vector comprising the gene of the invention operably linked to the selected promoter is constructed. This plasmid DNA plus plasmid DNA containing a PAT selectable marker is precipitated onto 1.1 μm (average diameter) tungsten pellets using a CaCl 2 precipitation procedure as follows: 100 μl prepared tungsten particles in water, 10 μl (1 μg) DNA in TrisEDTA buffer (1 μg total), 100 μl 2.5 M CaCl 2 , 10 μl 0.1 M spermidine. Each reagent is added sequentially to the tungsten particle suspension, while maintained on the multitube vortexer. The final mixture is sonicated briefly and allowed to incubate under constant vortexing for 10 minutes. After the precipitation period, the tubes are centrifuged briefly, liquid removed, washed with 500 ml 100% ethanol, and centrifuged for 30 seconds. Again the liquid is removed, and 105 μl 100% ethanol is added to the final tungsten particle pellet. For particle gun bombardment, the tungsten/DNA particles are briefly sonicated and 10 μl spotted onto the center of each macrocarrier and allowed to dry about 2 minutes before bombardment. The sample plates are bombarded at level #4 in particle gun #HE34-1 or #HE34-2. All samples receive a single shot at 650 PSI, with a total of ten aliquots taken from each tube of prepared particles/DNA. Following bombardment, the embryos are kept on 560Y medium, an N6 based medium, for 2 days, then transferred to 560R selection medium, an N6 based medium containing 3 mg/liter Bialaphos, and subcultured every 2 weeks. After approximately 10 weeks of selection, selection-resistant callus clones are sampled for PCR and activity of the gene of interest. Positive lines are transferred to 288J medium, an N6 based medium with lower sucrose and hormone levels, to initiate plant regeneration. Following somatic embryo maturation (2-4 weeks), well-developed somatic embryos are transferred to medium for germination and transferred to the lighted culture room. Approximately 7-10 days later, developing plantlets are transferred to medium in tubes for 7-10 days until plantlets are well established. Plants are then transferred to inserts in flats (equivalent to 2.5″ pot) containing potting soil and grown for 1 week in a growth chamber, subsequently grown an additional 1-2 weeks in the greenhouse, then transferred to classic 600 pots (1.6 gallon) and grown to maturity. Plants are monitored for expression of the gene of interest.
Example 9
[0220] Transformation of Callus and Regeneration of Corn Plants—Particle Gun.
[0221] Type II Callus Isolation and Maintenance.
[0222] After 10-21 days, type II callus is initiated from the scutellum and appears as a friable, embryogenic outgrowth of rapidly dividing cells. Callus is subcultured every 5-10 days and maintained on N6 medium supplemented with 1 mg/L 2,4-D (CM). These cultures are used in transformation experiments from 5 to 12 weeks after initiation.
[0223] Preparation of Callus for Transformation.
[0224] Proembryogenic type II callus is transferred to #4 Whatman filter paper on CM media. The CM plates with callus is wrapped with parafilm and incubated in the dark Conviron growth chamber (45% humidity, 27-28° C.) for two days before bombardment. Prior to bombardment, the osmotic plates are left partially ajar for thirty minutes in the laminar flow hood to allow moisture on the tissue to dissipate.
[0225] Gold Particle Preparation
[0226] Sixty mg of 0.6 micron gold is weighed out in a siliconized eppendorf tube (Axgen Microtubes—1.7 ml clear tube). The tube is left stationary for 15 minutes and spun down. The pellet is rinsed with sterile water three more times. Subsequently, one ml of sterile water is added to the gold pellet and vortexed for 10 minutes. The gold particles are divided into 50 ul aliquots.
[0227] DNA/Gold Preparation
[0228] Fifty μL of 0.6 micron gold in sterile dd H2O. A 2:1 molar ratio of trait gene:bar gene (usually ˜5-10 ug in total DNA) is added and vortexed. Subsequently, fifty μL of 2.5 M CaCl 2 is added quickly into the suspension and vortexed followed by the addition of 20 μL of 0.1 M spermidine and vortexed and spun down. The pellet is rinsed 3× in 100% ethanol. The pellet is gently resuspended by tapping the side of the eppendorf tube several times. The DNA prep is stored in the 20° C. freezer.
[0229] Loading of the Macrocarrier
[0230] The DNA/gold prep is thawed and sonicated (2 strokes) in the Branson 200 Ultrasonic cleaner prior to the addition to macrocarriers. The suspension is mixed well by pipetting in and out. Immediately, 6 μl of DNA/gold suspension is dispensed quickly to the center of each macrocarrier. Once the DNA prep is dried onto the macrocarrier, the PDS-100/He Gun is used to bombard the maize callus cells with the DNA-coated gold particles.
[0231] Particle Gun Parameters.
[0232] Plates containing callus are the bombarded with the PDS-1000/He Gun using the following parameters: 1) DNA precipitated onto 0.6 μM Gold particles; 2) 8 cm distance from stopping screen; 3) 27-29 inches Hg vacuum; 4) 1050-1100 PSI He pressure.
[0233] Selection of Transgenic Callus Lines.
[0234] After 3-4 days of incubation in the dark chamber the callus is transferred (3-4 mm clumps) onto media containing 3-5 ppm bialaphos (SM3 or SM5). The SM plates are incubated in the dark at 27° C. for ˜7-14 days. Thereafter, all callus is transferred onto SM (5 ppm bialaphos) keeping track of unique lines as above. Each clump may be split into several pieces at this transfer.
[0235] Regeneration of Transgenic Maize Plants.
[0236] Callus events are isolated onto fresh SM medium, sampled for PCR (polymerase chain reaction) and placed on first-stage regeneration media (RM31). After 10-14 days, the proembryogenic callus are transferred onto fresh RM3 plates and placed in the light chamber at 26° C. Plantlets approximately 2-3 cm are removed and transfer to RM4 media tubs. After 1-2 weeks plants from RM4 are potted to a maximum of two plantlets per pot. The pots are then placed in the Conviron growth chamber (photolight=20 hours, humidity=65%, temperature=24° C.) and watered with Roots2 solution. Plants (˜20 cm tall) are tested for expression of the bar gene by performing a 2% basta swipe test.
Example 10
[0237] Analysis of Fatty Acid Content and Composition by Gas Chromatography (GC)
[0238] Fatty acid (FA) determination was done from a total of 300-400 mg of tissue lyophilized for 24 hours. The tissue was then ground using a FastPrep mill (Biol101) at 4.5 speed and 20 seconds in the presence of 0.5 ml of 2.5% Sulfuric Acid+97.5% Methanol and Heptadecanoic acid (17:0, stock 10 mg/ml in Tuloene) as an external standard. Thereafter, another 0.5 ml 2.5% Sulfuric Acid +97.5% Methanol was used to wash each tube and incubate in 95° C. for 1 hour for transesterification. The tubes were removed from the water bath and allowed to cool down to RT. FAs were extracted in one volume of heptane:H 2 O (1:1) and cleared by centrifugation. The supernatant (50 ul) containing the fatty acid methyl esters were loaded into a Hewlett Packard 6890 gas chromatograph fitted with a 30 m×0.32 mm Omegawax column and the separated peaks were analyzed and characterized.
Example 11
[0239] Lec 1 Over-Expression Leads to Altered Fatty Acid Accumulation in Maize Somatic Embryos
[0240] The ubiquitin promoter (Christensen et al (1992) Plant Mol Biol 18:675-89) was used to drive Hap3/Lec1 expression (outlined in Example 7) in maize embryogenic callus to test what phenotype would arise from over-expression of Lec1 in somatic embryos. Transformation of the construct into maize embryogenic callus and generation of somatic embryos is outlined in Example 9.
[0241] More than ten different events were analysed by GC for fatty acid content/composition and compared to controls transformed with the selectable marker (BAR gene) plasmid alone. A pool of three embryos each from XX different events showed that the somatic embryos overexpressing Lec1 contain elevated fatty acid content (average 119% increase over control) with no significant alteration in fatty acid composition when compared to the control somatic embryos (FIG. 1).
Example 12
[0242] Nuclear Magnetic Resonance (NMR) ANALYSIS
[0243] Seed are imbibed in distilled water for 12-24 hours at 4° C. The embryo is dissected away and stored in a 48 well plate. The samples are lyophilized over-night in a Virtis 24×48 lyophilizer. The NMR (Process Control Technologies—PCT (Ft. Collins, Colo.) is set up as per the manufacturer's instructions. The NMR is calibrated using a series of 5 mm NMR tubes containing precisely measured amounts of corn oil (Mazola). The calibration standards are 3, 6, 9, 12, 15, 18, 21, 27, 33, and 40 mg of oil.
Example 13
[0244] Lec 1 Over-Expression Leads to Altered Oil Accumulation in Maize Kernels
[0245] The Hap3/Lec1 expression construct with the oleosin promoter (outlined in Example 7) was introduced into maize to test what phenotype would arise from seed specific over-expression. Transformation of the construct into maize was accomplished using Agrobacterium tumefaciens as follows.
[0246] Freshly isolated immature embryos of maize, about 10 days after pollination (DAP), are incubated with the Agrobacterium. The preferred genotype for transformation is the highly transformable genotype Hi-II (Armstrong, C. L., 1991, Development and Availability of Germplasm with High Type II Culture Formation Response, Maize Genetics Cooperation Newsletter, 65:92-93). An F 1 hybrid created by crossing with an Hi-II with an elite inbred may also be used. After Agrobacterium treatment of immature embryos, the embryos are cultured on medium containing toxic levels of herbicide. Only those cells which receive the herbicide-resistance gene, and the linked gene(s), grow on selective medium. Transgenic events so selected are propagated and regenerated to whole plants, produce seed, and transmit transgenes to progeny.
[0247] The engineered Agrobacterium tumefaciens LBA4404 is constructed as per U.S. Pat. No. 5,591,616 to contain the linked gene(s) and the selectable marker gene. Typically either BAR (D'Halluin et al (1992) Methods Enzymol. 216:415-426) or PAT (Wohileben et al (1988) Gene 70:25-37) may be used.
[0248] To use the engineered vector in plant transformation, a master plate of single bacterial colonies is first prepared by inoculating the bacteria on minimal AB medium and then incubating the bacteria plate inverted at 28° C. in darkness for about 3 days. A working plate is then prepared by selecting a single colony from the plate of minimal A medium and streaking it across a plate of YP medium. The YP-medium bacterial plate is then incubated inverted at 28° C. in darkness for 1-2 days.
[0249] Agrobacterium for plant transfection and co-cultivation is prepared 1 day prior to transformation. Into 30 ml of minimal A medium in a flask is placed 50 μg/ml spectinomycin (or appropriate bacterial antibiotic depending on marker in co-integrate), 100 μM acetosyringone, and about a ⅛ loopful of Agrobacterium from a 1 to 2-day-old working plate. The Agrobacterium is then grown at 28° C. at 200 rpm in darkness overnight (about 14 hours). In mid-log phase, the Agrobacterium is harvested and resuspended at 3 to 5×10 8 CFU/ml in 561 Q medium+100 μM acetosyringone using standard microbial techniques and standard curves.
[0250] Immature Embryo Preparation
[0251] Nine to ten days after controlled pollination of a corn plant, developing immature embryos are opaque and 1-1.5 mm long and are the appropriate size for Agro-infection. The husked ears are sterilized in 50% commercial bleach and 1 drop Tween for 30 minutes, and then rinsed twice with sterile water. The immature embryos are aseptically removed from the caryopsis and placed into 2 ml of sterile holding solution comprising of 561Q+100 μM acetosyringone.
[0252] Agrobacterium Infection and Co-cultivation of Embryos
[0253] Holding solution is decanted from excised immature embryos and replaced with prepared Agrobacterium. Following gentle mixing and incubation for about 5 minutes, the Agrobacterium is decanted from the immature embryos. Immature embryos are then moved to a plate of 562P medium, scutellum surface upwards, and incubated at 20° C. for 3 days in darkness followed by incubation at 28° C. for 3 days in darkness on medium 562P+100 mg/ml carbenecillin (see U.S. Pat. No. 5,981,840).
[0254] Selection of Transgenic Events
[0255] Following incubation, the immature embryos are transferred to 563O medium for selection of events. The transforming DNA possesses a herbicide-resistance gene, in this example the PAT gene, which confers resistance to bialaphos. At 10- to 14-day intervals, embryos are transferred to 5630 medium. Actively growing putative transgenic embryogenic tissue is visible in 6-8 weeks.
[0256] Regeneration of T 0 Plants
[0257] Transgenic embryogenic tissue is transferred to 288W medium and incubated at 28° C. in darkness until somatic embryos matured, or about 10 to 18 days. Individual matured somatic embryos with well-defined scutellum and coleoptile are transferred to 272 embryo germination medium and incubated at 28° C. in the light. After shoots and roots emerge, individual plants are potted in soil and hardened-off using typical horticultural methods.
[0258] Confirmation of Transformation
[0259] Putative transgenic events are subjected to analysis to confirm their transgenic nature. Events are tested for the presence of Lec1 by PCR amplification. Additionally, T 0 plants are painted with bialaphos herbicide. The subsequent lack of a herbicide-injury lesion indicates the presence and action of the herbicide resistance gene. The plants are monitored and scored for altered Lec1 expression and/or phenotype such as increased organic sulfur compounds.
[0260] Media Recipes
[0261] Medium 561 Q contains the following ingredients: 950.000 ml of D-I Water, Filtered; 4.000 g of Chu (N6) Basal Salts (Sigma C-1416); 1.000 ml of Eriksson's Vitamin Mix (1000+Sigma-1511); 1.250 ml of Thiamine.HCL.4 mg/ml; 3.000 ml of 2, 4-D 0.5 mg/ml (No. 2A); 0.690 g of L-proline; 68.500 g of Sucrose; and 36.000 g of Glucose. Directions are: dissolve ingredients in polished deionized water in sequence; adjust pH to 5.2 w/KOH; Q.S. to volume with polished deionized water after adjusting pH; and filter sterilize (do not autoclave).
[0262] Medium 562 P contains the following ingredients: 950.000 ml of D-I Water, Filtered; 4.000 g of Chu (N6) Basal Salts (Sigma C-1416); 1.000 ml of Eriksson's Vitamin Mix (1000×Sigma-1511); 1.250 ml of Thiamine.HCL.4 mg/ml; 4.000 ml of 2, 4-D 0.5 mg/ml; 0.690 g of L-proline; 30.000 g of Sucrose; 3.000 g of Gelrite, which is added after Q.S. to volume; 0.425 ml of Silver Nitrate 2 mg/ml #; and 1.000 ml of Aceto Syringone 100 mM #. Directions are: dissolve ingredients in polished deionized water in sequence; adjust pH to 5.8 w/KOH; Q.S. to volume with polished deionized water after adjusting pH; and sterilize and cool to 60° C. Ingredients designated with a # are added after sterilizing and cooling to temperature.
[0263] Medium 563 O contains the following ingredients: 950.000 ml of D-I Water, Filtered; 4.000 g of Chu (N6) Basal Salts (Sigma C-1416); 1.000 ml of Eriksson's Vitamin Mix (1000×Sigma-1511); 1.250 ml of Thiamine.HCL.4 mg/ml; 30.000 g of Sucrose; 3.000 ml of 2, 4-D 0.5 mg/ml (No. 2A); 0.690 g of L-proline; 0.500 g of Mes Buffer; 8.000 g of Agar (Sigma A-7049, Purified), which is added after Q.S. to volume; 0.425 ml of Silver Nitrate 2 mg/ml #; 3.000 ml of Bialaphos 1 mg/ml #; and 2.000 ml of Agribio Carbenicillin 50 mg/ml #. Directions are: dissolve ingredients in polished deionized water in sequence; adjust to pH 5.8 w/koh; Q.S. to volume with polished deionized water after adjusting pH; sterilize and cool to 60° C. Ingredients designated with a # are added after sterilizing and cooling to temperature.
[0264] Medium 288 W contains the following ingredients: 950.000 ml of D-I H 2 O; 4.300 g of MS Salts; 0.100 g of Myo-Inositol; 5.000 ml of MS Vitamins Stock Solution (No. 36J); 1.000 ml of Zeatin.5 mg/ml; 60.000 g of Sucrose; 8.000 g of Agar (Sigma A-7049, Purified), which is added after Q.S. to volume; 2.000 ml of IAA 0.5 mg/ml #; 1.000 ml of 0.1 Mm ABA #; 3.000 ml of Bialaphos 1 mg/ml #; and 2.000 ml of Agribio Carbenicillin 50 mg/ml #. Directions are: dissolve ingredients in polished deionized water in sequence; adjust to pH 5.6; Q.S. to volume with polished deionized water after adjusting pH; sterilize and cool to 60° C. Add 3.5 g/L of Gelrite for cell biology. Ingredients designated with a # are added after sterilizing and cooling to temperature.
[0265] Medium 272 contains the following ingredients: 950.000 ml of deionized water; 4.300 g of MS Salts; 0.100 g of Myo-Inositol; 5.000 of MS Vitamins Stock Solution; 40.000 g of Sucrose; and 1.500 g of Gelrite, which is added after Q.S. to volume. Directions are: dissolve ingredients in polished deionized water in sequence; adjust to pH 5.6; Q.S. to volume with polished deionized water after adjusting pH; and sterilize and cool to 60° C.
[0266] Medium minimal A contains the following ingredients: 950.000 ml of deionized water; 10.500 g of potassium phosphate dibasic K2HPO4; 4.500 g of potassium phosphate monobasic KH2PO4; 1.000 g of ammonium sulfate; 0.500 g of sodium citrate dihydrate; 10.000 ml of sucrose 20% solution #; and 1.000 ml of 1 M magnesium sulfate #. Directions are: dissolve ingredients in polished deionized water in sequence; Q.S. to volume with deionized water; sterilize and cool to 60° C. Ingredients designated with a # are added after sterilizing and cooling to temperature.
[0267] Medium minimal AB contains the following ingredients: 850.000 ml of deionized water; 50.000 ml of stock solution 800A; 9 g of Phytagar which is added after Q.S. to volume; 50.000 ml of stock solution 800B #; 5.000 g of glucose #; and 2.000 ml of spectinomycin 50/mg/ml stock #. Directions are: dissolve ingredients in polished deionized water in sequence; Q.S. to volume with polished deionized water less 100 ml per liter; sterilize and cool to 60° C. Ingredients designated with a # are added after sterilizing and cooling to temperature. Stock solution 800A contains the following ingredients: 950.000 ml of deionized water; 60.000 g of potassium phosphate dibasic K2HPO4; and 20.000 g of sodium phos. monobasic, hydrous. Directions are: dissolve ingredients in polished deionized water in sequence; adjust pH to 7.0 with potassium hydroxide; Q.S. to volume with polished deionized water after adjusting pH; and sterilize and cool to 60° C. Stock solution 800B contains the following ingredients: 950.000 ml of deionized water; 20.000 g of ammonium chloride; 6.000 g of magnesium sulfate 7-H 2 O, MgSO 4 , 7 H 2 O; 3.000 g of potassium chloride; 0.200 g of calcium chloride (anhydrate); and 0.050 g of ferrous sulfate 7-hydrate. Directions are: dissolve ingredients in polished deionized water in sequence; Q.S. to volume with polished deionized water; and sterilize and cool to 60° C.
[0268] Medium minimal YP contains the following ingredients: 950.000 ml of deionized water; 5.000 g of yeast extract (Difco); 10.000 g of peptone (Difco); 5.000 g of sodium chloride; 15.000 g of bacto-agar, which is added after Q.S. to volume; and 1.000 ml of spectinomycin 50 mg/ml stock #. Directions are: dissolve ingredients in polished deionized water in sequence; adjust pH to 6.8 with potassium hydroxide; Q.S. to volume with polished deionized water after adjusting pH; sterilize and cool to 60° C. Ingredients designated with a # are added after sterilizing and cooling to temperature.
[0269] More than twenty events producing segregating T1 seed were analyzed by NMR for embryo oil content (see Example 12). Six to twelve embryos analyzed for each of five different events showed that some embryos within each event contained elevated oil content. These results are shown in FIG. 2. The same embryos from these five events were analyzed by PCR to determine the presence or absence of the Lec1 construct. Embryos with high oil are always found to contain the Lec1 construct (darkly shaded bars), whereas embryos with normal levels of oil were typically found not to contain the Lec1 construct (cross-hatched bars). These data demonstrate the presence of the Lec1 gene does lead to increased oil in the embryo. It is believed that embryos containing sharply higher levels of oil were homozygous for the Lec1 construct, as these events were segregating 1:2:1. For these events, the oil concentration in the embryos containing the Lec1 construct greatly surpassed any increase previously achieved through enzymatic modification of the fatty acid biosynthetic pathway, with some embryos containing an average increase of 56% in embryo oil content (FIG. 2, Event 277267). Plants derived from seed that contained high oil exhibit some phenotypic changes in growth and development. There is an accumulation of additional leaves during early growth and development phase, and strong leaf curling throughout plant growth and development.
Example 14
[0270] Additional Promoters Coupled to Lec1 Also Result in Altered Maize Kernel Oil Accumulation
[0271] Other types of seed-specific promoters, the lipid transfer protein promoter and the gamma zein promoter, were also tested for their ability to alter oil accumulation in maize kernels when expressing Lec1. Transformation and analysis of these constructs was essentially the same as protocols outlined in Example 13. More than twenty events producing segregating T1 seed are analyzed by NMR for embryo oil content (see Example 12). Six to twelve embryos were analyzed for each event. Events containing embryos with high oil content were analyzed further. The same embryos from these events are analyzed by PCR to determine the presence or absence of the Lec1 construct. As with the oleosin promoter containing construct, all embryos with high oil contents are found to contain the Lec1 construct, whereas embryos with lower or normal oil contents are typically found not to contain the Lec1 construct. Like the events containing Lec1 and the oleosin promoter, the oil concentration in the embryo for these events also greatly surpass any increase previously achieved through enzymatic modification, with some embryos containing an average increase of more than 50% in embryo oil content.
[0272] Surprisingly, plants derived from seed containing high oil using this construct do not show the abnormal phenotype found for plants expressing Lec1 under the control of the oleosin promoter. It is believed that these data demonstrate that high oil can be achieved in the embryo without negative agronomic effects when the appropriate expression is employed.



1


222


1
638
DNA
Catalpa speciosa

unsure
(402)
n = A, C, G, or T


1

gtgctcttta aaattcacaa gtacatctga cctctacatc aacacacatt gactctaaat 60

tctctctcta aattctgtca acccccaaat tctagggttt tgttttaatt gtcatcagat 120

ttcgccttaa caggacacat tggttgattt ctttgggaga aattagggga gcatgcaatc 180

caagtcccag agcggcaacc aaggagaatc caacctttat aatgttccta actccaaagt 240

aaatccggat tcttggtgga ataatactgg gatataatcc ttttcctcaa caatgatggg 300

gtgggaaatg catcaagatt catcatccct agaacaatct gtgggatgga caagtcgcag 360

tctaaaggtg gtataaatga ggaagatgat gatactacca anacgatcac aaagttagta 420

cacctccggc tgccaagata gaaactatag gcaggagggc cgagctccag caagctccac 480

ctaccaatac atccaaagaa acaatgggat cgttaatcan ggccanagtt gagctgggng 540

gnatcagtag ctgggggnca aancctaaga tcatatacgg nggaagatgg aactaaggca 600

gcatggtcnc ccaattaang anagcacann anggtgga 638



2
77
PRT
Catalpa speciosa

UNSURE
(35)
Xaa = any amino acid


2

Met Gln Ser Lys Ser Gln Ser Gly Asn Gln Gly Glu Ser Asn Leu Tyr
1 5 10 15

Asn Val Pro Asn Ser Lys Val Asn Pro Asp Ser Trp Trp Asn Asn Thr
20 25 30

Gly Ile Xaa Ser Phe Ser Ser Thr Met Met Gly Gly Asn Ala Ser Arg
35 40 45

Phe Ile Ile Pro Arg Thr Ile Cys Gly Met Asp Lys Ser Gln Ser Lys
50 55 60

Gly Gly Ile Asn Glu Glu Asp Asp Asp Thr Thr Xaa Thr
65 70 75



3
441
DNA
Typha latifolia

unsure
(378)
n = A, C, G, or T


3

atttaggaga gagcttgagg tcgagaggag cagcagagga ggaaggaggc aggagaagca 60

aagggtttcg agaaagggga catgctcccc ttataaggac atggaaacca gaaagcaact 120

aggtcatcca ttgctgaagc aagactcatt ttcaaatgtc aactaatctg ttccaccaag 180

aagcatcggt aatgggtgaa gaccacctta gtgagaagca tacttcaaca caatctggga 240

atgctggtag ttatggaaat ataagggatg gttatccaaa atcagtatta tccttggcaa 300

atccagaagc tgcctttgta cctccgaaac ttgattgtag ccagtctttt acttgcatgc 360

catacccttt tgctgatnca tgctttggtg gtgtcatggc tgcatatggt tcgcnatgcc 420

tttattcaac aacaaatggt g 441



4
95
PRT
Typha latifolia

UNSURE
(75)
Xaa = any amino acid


4

Met Ser Thr Asn Leu Phe His Gln Glu Ala Ser Val Met Gly Glu Asp
1 5 10 15

His Leu Ser Glu Lys His Thr Ser Thr Gln Ser Gly Asn Ala Gly Ser
20 25 30

Tyr Gly Asn Ile Arg Asp Gly Tyr Pro Lys Ser Val Leu Ser Leu Ala
35 40 45

Asn Pro Glu Ala Ala Phe Val Pro Pro Lys Leu Asp Cys Ser Gln Ser
50 55 60

Phe Thr Cys Met Pro Tyr Pro Phe Ala Asp Xaa Cys Phe Gly Gly Val
65 70 75 80

Met Ala Ala Tyr Gly Ser Xaa Cys Leu Tyr Ser Thr Thr Asn Gly
85 90 95



5
849
DNA
Vitis sp.

5

ctgaggttgc agagacacca tggattccca ccaacggcca tgatttcctt ccaaactcct 60

accttttagg gtttattcct ctgctctcat cccacattag atttggggct aggggatttt 120

tgtttttctt ggtggaaaag aataatgccg actaaaccca aaattgagga tcggcggata 180

gaacctggtg gtaagagcaa tccgtcatca acagtctact cccaaccttg gtggcatggt 240

gttgggaaca atgccatctc cccagctgcc ttgggtggaa gcccatcaaa atcaacttca 300

gttgaacacc ttaacagtca tatcacgagc aatggtttcc aattacaagc taatggcagg 360

ctggatgatg gaactacctt taataaagga acacaaccta cggtagccct gcaatctgat 420

ggaaggaatg gacaggaaca ccagcacctc aatcctactg cttcctcaac actgccaatt 480

atgagtgaac atcttgaacc aaattcccaa atggaacttg ttggtcactc aattgtgttg 540

acatcatatc cgtatcaaga tccacataat gtggggatta tgacttctta tgggccacag 600

gctatggtat gcaaagaagt tggttgcatt tctgtgtgtt gtggtaacat tactgttggt 660

ggcactacca cttctgaaag tgatgcctca accttgaaaa ctagattctc ctgtactagg 720

gcctgcccct cttatagggg aggtcagcca ctgtagtgaa taatctgttt cataagaaaa 780

tcatcagttt ttatgtgaag gttccttctt ctagatttgg tctcgcccaa gaaaaaaaaa 840

aaaaaaaaa 849



6
154
PRT
Vitis sp.

6

Met Pro Thr Lys Pro Lys Ile Glu Asp Arg Arg Ile Glu Pro Gly Gly
1 5 10 15

Lys Ser Asn Pro Ser Ser Thr Val Tyr Ser Gln Pro Trp Trp His Gly
20 25 30

Val Gly Asn Asn Ala Ile Ser Pro Ala Ala Leu Gly Gly Ser Pro Ser
35 40 45

Lys Ser Thr Ser Val Glu His Leu Asn Ser His Ile Thr Ser Asn Gly
50 55 60

Phe Gln Leu Gln Ala Asn Gly Arg Leu Asp Asp Gly Thr Thr Phe Asn
65 70 75 80

Lys Gly Thr Gln Pro Thr Val Ala Leu Gln Ser Asp Gly Arg Asn Gly
85 90 95

Gln Glu His Gln His Leu Asn Pro Thr Ala Ser Ser Thr Leu Pro Ile
100 105 110

Met Ser Glu His Leu Glu Pro Asn Ser Gln Met Glu Leu Val Gly His
115 120 125

Ser Ile Val Leu Thr Ser Tyr Pro Tyr Gln Asp Pro His Asn Val Gly
130 135 140

Ile Met Thr Ser Tyr Gly Pro Gln Ala Met
145 150



7
1334
DNA
Vitis sp.

7

ctcatttgaa aatccgtaga ccgaaccatg gacttcgtat ccatcattct tctctctcca 60

tagctcctca attctagggt ttctctcact cttcttcctc tctgaatgga agctgtggac 120

aagaacaaaa gcatcctcag caagctgtat caatgatgcc tatgactatg gctgaatacc 180

accttgcacc accttcccag ctggaacttg ttggccactc aattgcgtgt gcatcatatc 240

catattctga accttattac acgggagtca ttcctgctta tggacctcag ggtttggtac 300

aatctcaatt tcttggtgtg aatgtggcta gaatggcttt gcctattgaa atggcagagg 360

aacctgttta tgtgaatgca aaacagtatc atgggattct gaggcgaaga caatcacggg 420

cgaaggccga gctggaaaaa aaactgataa aagttaggaa gccatatctt catgaatcaa 480

ggcaccagca tgctatgaga agggcaagag gatgtggagg ccgttttctc aacacaaaga 540

agcttgattc taatgcatcg tatgacatgc ctgacaaggg ctctgatcca gatgtaaacc 600

tttcaacacg acccatcagc tcatcagtct ctgaatctct gccctccaat tcttcccgaa 660

atgaggattc ccccaccagt catctagatg caagaggtcc ctctgtgcag gaattgcaca 720

ataggcaaac agcctcccat ggaaatggca acagctgtta tccacacaac cagggatttc 780

agttgtcgac ataccattcc cttaaagatg atcgcgtgga agaaggagac cacgcagggc 840

ggcagcatga gagaattctg gtgaataggg ccccccacag ggccctaacc atcaaatgaa 900

accttcgttg ctaagggatg aagggtcttt ccagcattgc tctgatctat tgcagatggc 960

atcagcttcc atgtgggctt gagggtgtca cagaagtggg ctagttcaaa tacaaaaata 1020

agtgaggagc atccttctgt gacttctact caagtatctg gtaacggatc cggatggcag 1080

cattgcaggg caaagctgga agcattaccc caaccaatca gagggggggg ggacccctgg 1140

cctatgtgtt gtattttcag gcaaatcatt cttggcttgt atttttcata ttcctgtgtt 1200

tgttggaccg ggggggaaag acagagagat tgggaatcgt ctaatttcac tcattacctt 1260

tttggaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1320

aaaaaaaaaa aaaa 1334



8
261
PRT
Vitis sp.

8

Cys Gly Gln Glu Gln Lys His Pro Gln Gln Ala Val Ser Met Met Pro
1 5 10 15

Met Thr Met Ala Glu Tyr His Leu Ala Pro Pro Ser Gln Leu Glu Leu
20 25 30

Val Gly His Ser Ile Ala Cys Ala Ser Tyr Pro Tyr Ser Glu Pro Tyr
35 40 45

Tyr Thr Gly Val Ile Pro Ala Tyr Gly Pro Gln Gly Leu Val Gln Ser
50 55 60

Gln Phe Leu Gly Val Asn Val Ala Arg Met Ala Leu Pro Ile Glu Met
65 70 75 80

Ala Glu Glu Pro Val Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu
85 90 95

Arg Arg Arg Gln Ser Arg Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile
100 105 110

Lys Val Arg Lys Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met
115 120 125

Arg Arg Ala Arg Gly Cys Gly Gly Arg Phe Leu Asn Thr Lys Lys Leu
130 135 140

Asp Ser Asn Ala Ser Tyr Asp Met Pro Asp Lys Gly Ser Asp Pro Asp
145 150 155 160

Val Asn Leu Ser Thr Arg Pro Ile Ser Ser Ser Val Ser Glu Ser Leu
165 170 175

Pro Ser Asn Ser Ser Arg Asn Glu Asp Ser Pro Thr Ser His Leu Asp
180 185 190

Ala Arg Gly Pro Ser Val Gln Glu Leu His Asn Arg Gln Thr Ala Ser
195 200 205

His Gly Asn Gly Asn Ser Cys Tyr Pro His Asn Gln Gly Phe Gln Leu
210 215 220

Ser Thr Tyr His Ser Leu Lys Asp Asp Arg Val Glu Glu Gly Asp His
225 230 235 240

Ala Gly Arg Gln His Glu Arg Ile Leu Val Asn Arg Ala Pro His Arg
245 250 255

Ala Leu Thr Ile Lys
260



9
987
DNA
Vitis sp.

9

gcacgaggga aggtcaaagt caaatgaagc cagttttctt tatggctaat ccagatgttg 60

tcttcaatcc ttcacaagtt gactatggcc attctgtgac tcatgttgca tatccttatg 120

ctgatcctta ccatgggggg ttagtggctg catatggtcc acatgctgtt attcagcccc 180

agctggtggg gatagcacct accagagtcc cactgccctt tgatattgca gaggatggac 240

ctatttttgt caatgcaaaa cagtatcatg gaattctcag gaggaggcag tcacgagcaa 300

agatggaggc ccagaacaaa cttgtcaaag cccgaaagcc atatctgcac gagtctcggc 360

atcttcatgc cctaaatagg gttagaggat ctggtggacg cttcctcagc acgaaaaagc 420

tccaagaacc ggactcaact tccaatgctg gctgtcatag tgtatctggc tctggtcatt 480

ttcaccagaa gggagacaca actgagcagc cggagcacag gttctcaggc atgtctcccc 540

acatgggtgg agccatgcaa ggtggtggcg gtgggactta tgggcaatgg agtcctgctc 600

ctggttgtcc ggtgagaagt cgataggaac aagatcgatg gagtcactgg tctgggcaat 660

tcatccttgg ctttgttact ttcgtttcat gcgtgttaag aagataaaca catcaaactt 720

catggtgtag tagaaatact ctgcctttcc catttccaaa tgcatacatt ttggctctgt 780

aaacatggtt gagaagaggc tatgcttgaa actctctgtt tgtgaaccat tgttttgttt 840

tttcaagaca atgtgagata ttggttcacc ggtattttgt ttgttgctta cagaaagcaa 900

accctgcctt ttgtgcttaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 960

aaaaaaaaaa aaaaaaaaaa aaaaaaa 987



10
205
PRT
Vitis sp.

10

Glu Gly Gln Ser Gln Met Lys Pro Val Phe Phe Met Ala Asn Pro Asp
1 5 10 15

Val Val Phe Asn Pro Ser Gln Val Asp Tyr Gly His Ser Val Thr His
20 25 30

Val Ala Tyr Pro Tyr Ala Asp Pro Tyr His Gly Gly Leu Val Ala Ala
35 40 45

Tyr Gly Pro His Ala Val Ile Gln Pro Gln Leu Val Gly Ile Ala Pro
50 55 60

Thr Arg Val Pro Leu Pro Phe Asp Ile Ala Glu Asp Gly Pro Ile Phe
65 70 75 80

Val Asn Ala Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln Ser Arg
85 90 95

Ala Lys Met Glu Ala Gln Asn Lys Leu Val Lys Ala Arg Lys Pro Tyr
100 105 110

Leu His Glu Ser Arg His Leu His Ala Leu Asn Arg Val Arg Gly Ser
115 120 125

Gly Gly Arg Phe Leu Ser Thr Lys Lys Leu Gln Glu Pro Asp Ser Thr
130 135 140

Ser Asn Ala Gly Cys His Ser Val Ser Gly Ser Gly His Phe His Gln
145 150 155 160

Lys Gly Asp Thr Thr Glu Gln Pro Glu His Arg Phe Ser Gly Met Ser
165 170 175

Pro His Met Gly Gly Ala Met Gln Gly Gly Gly Gly Gly Thr Tyr Gly
180 185 190

Gln Trp Ser Pro Ala Pro Gly Cys Pro Val Arg Ser Arg
195 200 205



11
1256
DNA
Zea mays

11

gcacgagctc tgtctgtgtg cgagcgcaag agaaagggag tcagagagag agggaggaga 60

ccttgcagag gagcgaagca agcaaggtgg gaaagaggca gcaagggcgg cgggctgccg 120

gaaggggaac atgctccctc ctcatctcac agtacgaact gaaaaacaag agtaaagaat 180

ttccgtgaga tgagacagaa tggcgcggtg atgattcagt ttggccatca gatgcctgat 240

tacgactccc cggctaccca gtcaaccagt gagacgagcc atcaagaagc gtctggaatg 300

agcgaaggga gcctcaacga gcataataat gaccattcag gcaaccttga tgggtactcg 360

aagagtgacg aaaacaagat gatgtcagcg ttatccctgg gcaatccgga aacagcttac 420

gcacataatc cgaagcctga ccgtactcag tccttcgcca tatcataccc atatgccgat 480

ccatactacg gtggcgcggt ggcagcagct tatggcccgc atgctatcat gcaccctcag 540

ctggttggca tggttccgtc ctctcgagtg ccactgccga tcgagccagc cgctgaagag 600

cccatctatg tcaacgcgaa gcagtaccac gctattctcc ggaggagaca gctccgtgca 660

aagctagagg cggaaaacaa gctcgtgaaa agccgcaagc cgtacctcca cgagtctcgg 720

cacctgcacg cgatgaagag agctcgggga acaggcgggc ggttcctgaa cacgaagcag 780

cagccggagt cccccggcag cggcggctcc tcggacgcgc aacgcgtgcc cgcgaccgcg 840

agcggcggcc tgttcacgaa gcatgagcac agcctgccgc ccggcggtcg ccaccactat 900

cacgcgagag ggggcggtga gtagggagcc ccgacactgg caactcatcc ttggcttatc 960

agcgattcga ctcggctctc gctcgtctga aactgaactc tctgcaacta ctgtaactgt 1020

aactaaactg ggtgtgcccg gattggcggt cgttctgttc tactactact agtaccttag 1080

tacctgctac gcgtcgttgg gtctggacta gagagccgtg ctggttcttt gatgaacttg 1140

gctggacttg aggtgttgac tagcgcgaaa ctgagttcca tgtaaacttt tgcttcaaga 1200

ccgatgactg gcggcataat aagtagcagt aataaccaaa aaaaaaaaaa aaaaaa 1256



12
244
PRT
Zea mays

12

Met Arg Gln Asn Gly Ala Val Met Ile Gln Phe Gly His Gln Met Pro
1 5 10 15

Asp Tyr Asp Ser Pro Ala Thr Gln Ser Thr Ser Glu Thr Ser His Gln
20 25 30

Glu Ala Ser Gly Met Ser Glu Gly Ser Leu Asn Glu His Asn Asn Asp
35 40 45

His Ser Gly Asn Leu Asp Gly Tyr Ser Lys Ser Asp Glu Asn Lys Met
50 55 60

Met Ser Ala Leu Ser Leu Gly Asn Pro Glu Thr Ala Tyr Ala His Asn
65 70 75 80

Pro Lys Pro Asp Arg Thr Gln Ser Phe Ala Ile Ser Tyr Pro Tyr Ala
85 90 95

Asp Pro Tyr Tyr Gly Gly Ala Val Ala Ala Ala Tyr Gly Pro His Ala
100 105 110

Ile Met His Pro Gln Leu Val Gly Met Val Pro Ser Ser Arg Val Pro
115 120 125

Leu Pro Ile Glu Pro Ala Ala Glu Glu Pro Ile Tyr Val Asn Ala Lys
130 135 140

Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu Glu
145 150 155 160

Ala Glu Asn Lys Leu Val Lys Ser Arg Lys Pro Tyr Leu His Glu Ser
165 170 175

Arg His Leu His Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg Phe
180 185 190

Leu Asn Thr Lys Gln Gln Pro Glu Ser Pro Gly Ser Gly Gly Ser Ser
195 200 205

Asp Ala Gln Arg Val Pro Ala Thr Ala Ser Gly Gly Leu Phe Thr Lys
210 215 220

His Glu His Ser Leu Pro Pro Gly Gly Arg His His Tyr His Ala Arg
225 230 235 240

Gly Gly Gly Glu



13
1203
DNA
Zea mays

13

ccacgcgtcc ggcaagagaa agggagtcag agagagagag agagggagga gaccttgcag 60

aggagcgaag caagcaaggt gggaaagagg cagcagcaag ggcggcgggc tgccggaagg 120

ggaacatgct ccctcctcat ctcacagaga atggcgcggt gatgattcag tttggccatc 180

agatgcctga ttacgactcc ccggctaccc agtcaaccag tgagacgagc catcaagaag 240

cgtctggaat gagcgaaggg agcctcaacg agcataataa tgaccattca ggcaaccttg 300

atgggtactc gaagagtgac gaaaacaaga tgatgtcagc gttatccctg ggcaatccgg 360

aaacagctta cgcacataat ccgaagcctg accgtactca gtccttcgcc atatcatacc 420

catatgccga tccatactac ggtggcgcgg tggcagcagc ttatggcccg catgctatca 480

tgcaccctca gctggttggc atggttccgt cctctcgagt gccactgccg atcgagccag 540

ccgctgaaga gcccatctat gtcaacgcga agcagtacca cgctattctc cggaggagac 600

agctccgtgc aaagctagag gcggaaaaca agctcgtgaa aagccgcaag ccgtacctcc 660

acgagtctcg gcacctgcac gcgatgaaga gagctcgggg aacaggcggg cggttcctga 720

acacgaagca gcagccggag tcccccggca gcggcggctc ctcggacgcg caacgcgtgc 780

ccgcgaccgc gagcggcggc ctgttcacga agcatgagca cagcctgccg cccggcggtc 840

gccaccacta tcacgcgaga gggggcggtg agtagggagc cccgacactg gcaactcatc 900

cttggcttat cagcgattcg actcggctct ccctcgtctg aaactgaact ctctgcaact 960

actgtaactg taactaaact gggtgtgccc ggattggcgg tcgttctgtt ctactactag 1020

tacctgctac gcgtcgttgg gttgggtctg gactagagag cgtgctggtt ctttgatgaa 1080

cttggctgga cttgagggtg ttgactagcg cgaagctgag ttccatgtaa aacttttgct 1140

tcaagaccga tgactggcgg cataataagt agcagtaata accaaaaaaa aaaaaaaaaa 1200

aag 1203



14
288
PRT
Zea mays

14

Pro Ala Arg Glu Arg Glu Ser Glu Arg Glu Arg Glu Gly Gly Asp Leu
1 5 10 15

Ala Glu Glu Arg Ser Lys Gln Gly Gly Lys Glu Ala Ala Ala Arg Ala
20 25 30

Ala Gly Cys Arg Lys Gly Asn Met Leu Pro Pro His Leu Thr Glu Asn
35 40 45

Gly Ala Val Met Ile Gln Phe Gly His Gln Met Pro Asp Tyr Asp Ser
50 55 60

Pro Ala Thr Gln Ser Thr Ser Glu Thr Ser His Gln Glu Ala Ser Gly
65 70 75 80

Met Ser Glu Gly Ser Leu Asn Glu His Asn Asn Asp His Ser Gly Asn
85 90 95

Leu Asp Gly Tyr Ser Lys Ser Asp Glu Asn Lys Met Met Ser Ala Leu
100 105 110

Ser Leu Gly Asn Pro Glu Thr Ala Tyr Ala His Asn Pro Lys Pro Asp
115 120 125

Arg Thr Gln Ser Phe Ala Ile Ser Tyr Pro Tyr Ala Asp Pro Tyr Tyr
130 135 140

Gly Gly Ala Val Ala Ala Ala Tyr Gly Pro His Ala Ile Met His Pro
145 150 155 160

Gln Leu Val Gly Met Val Pro Ser Ser Arg Val Pro Leu Pro Ile Glu
165 170 175

Pro Ala Ala Glu Glu Pro Ile Tyr Val Asn Ala Lys Gln Tyr His Ala
180 185 190

Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu Glu Ala Glu Asn Lys
195 200 205

Leu Val Lys Ser Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu His
210 215 220

Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg Phe Leu Asn Thr Lys
225 230 235 240

Gln Gln Pro Glu Ser Pro Gly Ser Gly Gly Ser Ser Asp Ala Gln Arg
245 250 255

Val Pro Ala Thr Ala Ser Gly Gly Leu Phe Thr Lys His Glu His Ser
260 265 270

Leu Pro Pro Gly Gly Arg His His Tyr His Ala Arg Gly Gly Gly Glu
275 280 285



15
1301
DNA
Zea mays

15

gcacgagcca gtgcgacggc cacggcctga gcggcgctgc cagcaaggcg gctagtatga 60

gcagcatgga gtcgcggccg ggccgaacga acctggtgga gcccataggg cacggcgccg 120

cgctgccgtc cggcggccag gcagtgcagc cgtggtggac gagctccggg gctgtgctcg 180

gtgcagtctc gccagccgtc gtggcggtgg cgcccgggag cgggacgggg attagcctgt 240

cgagcagccc ggcaggtggt agtggtggtg gcggcgcggc taaaggagcc gcgagtgacg 300

agagcagcga ggattcacgg agatctgggg aaccaaaaga tggaagcgct agtcaagaaa 360

agaaccatgc cacatcgcag atacccgctc tggcgccaga gtatttggca ccatactcgc 420

agctggaact gaaccaatca attgcttctg cagcatatca gtacccagat ccttactatg 480

caggcatggt tgctccctat ggaagtcatg ctgtggctca ttttcagcta cctggactaa 540

ctcaatctcg aatgccatta cctcttgaag tatccgagga gcctgtttat gtaaatgcca 600

agcagtacca tggtatctta agacgacggc agtcccgtgc taaggctgaa cttgagaaaa 660

aggtggtcaa agccagaaag ccataccttc acgagtctcg tcatcagcac gcgatgagga 720

gggcaagagg aaacggggga cgcttcctga acacaaagaa aagtgacagt ggtgctccca 780

atggaggcga aaacgccgag catctccatg tccctcccga cttactacag ctacgacaga 840

acgaggcttg aagtagcggt atggctctgg catccttgaa cagcagttcc tgtccacggg 900

cgtaggcatt cgagaccgga ttcatatagc tctccacagc atacgcgcag ccatctctgc 960

ggtaacgcac gttctcctga acgagctttg tagcgagata ggtatgcaag tgcaatctgg 1020

gcgcaggaat ccatcatcaa gtgcccaatg cccatggggt aggtacgctg tttcaggcaa 1080

ttcattcttg gctttcacgt tccacccttg tgtaactggt gtgttgtaaa tgtgtggaaa 1140

actaagcttg tgctctgtat cgggccgttc agcggaactg caaaacgcct gtataattaa 1200

gatcgaactt tggattaact cggtaatgct ttgtctggtt ttcttttaaa aaaaaaaaaa 1260

aaaaaaaaaa aaaaaaaaaa aacaaaaaaa aaaaaaaaaa a 1301



16
264
PRT
Zea mays

16

Met Ser Ser Met Glu Ser Arg Pro Gly Arg Thr Asn Leu Val Glu Pro
1 5 10 15

Ile Gly His Gly Ala Ala Leu Pro Ser Gly Gly Gln Ala Val Gln Pro
20 25 30

Trp Trp Thr Ser Ser Gly Ala Val Leu Gly Ala Val Ser Pro Ala Val
35 40 45

Val Ala Val Ala Pro Gly Ser Gly Thr Gly Ile Ser Leu Ser Ser Ser
50 55 60

Pro Ala Gly Gly Ser Gly Gly Gly Gly Ala Ala Lys Gly Ala Ala Ser
65 70 75 80

Asp Glu Ser Ser Glu Asp Ser Arg Arg Ser Gly Glu Pro Lys Asp Gly
85 90 95

Ser Ala Ser Gln Glu Lys Asn His Ala Thr Ser Gln Ile Pro Ala Leu
100 105 110

Ala Pro Glu Tyr Leu Ala Pro Tyr Ser Gln Leu Glu Leu Asn Gln Ser
115 120 125

Ile Ala Ser Ala Ala Tyr Gln Tyr Pro Asp Pro Tyr Tyr Ala Gly Met
130 135 140

Val Ala Pro Tyr Gly Ser His Ala Val Ala His Phe Gln Leu Pro Gly
145 150 155 160

Leu Thr Gln Ser Arg Met Pro Leu Pro Leu Glu Val Ser Glu Glu Pro
165 170 175

Val Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln
180 185 190

Ser Arg Ala Lys Ala Glu Leu Glu Lys Lys Val Val Lys Ala Arg Lys
195 200 205

Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg Ala Arg
210 215 220

Gly Asn Gly Gly Arg Phe Leu Asn Thr Lys Lys Ser Asp Ser Gly Ala
225 230 235 240

Pro Asn Gly Gly Glu Asn Ala Glu His Leu His Val Pro Pro Asp Leu
245 250 255

Leu Gln Leu Arg Gln Asn Glu Ala
260



17
1258
DNA
Zea mays

17

gcacgaggcc acgccgccgg ccacgcccca gacgaccccg cccgccgccg ccgcctcccg 60

ctccctccgc gcgcagccct cgtccggccg cccgggtccg agcgcgctcg ctcctcctcc 120

ccacgtcgga cagtttaagt gtggcttcat tgcatgagta gttgcagtta gcgtggcttt 180

tctccgtgct tgctcctggt cgtgctttgc cttgcaaagg aaggaatcat gacatctgtt 240

gttcacagtg tttcaggtga ccacagggct gaggatcaaa atcaacagaa gaagcaagct 300

gaacctgggg accagcaaga agccccagtt actagttcag atagccaacc aacagtaggc 360

acaccatcaa cagattatgt ggcaccctat gcccctcatg acatgagcca tgcaatgggt 420

caatacgctt atccaaatat tgacccatac tatggaagcc tttatgcagc agcttacggt 480

ggacagccat tgatgcatcc accgttagtt ggaatgcatc cggctggctt acctttgcct 540

accgatgcaa ttgaagagcc tgtgtatgta aatgcaaagc aatacaatgc catattaaga 600

cggcgtcaat ctcgggctaa agctgaatca gaacgaaagc ttatcaaggg gcgtaagccc 660

tatctccatg agtcacgtca tcagcatgcc ttgaaaaggg ccaggggagc tggaggtcgg 720

tttctcaact caaagtcaga tgacaaggaa gagaactccg actcgagtca caaagagaat 780

cagaacggag ttgcgcccca caggagcggc caaccgtcaa cccctccgtc tcccaacggt 840

gcatcgtcag ctaatcaggg caggcagtcg tgaatgatgg atgattcaaa actcacagct 900

gaagagattt cagcccctga gctagatatg gcagcagttt tgtacagaaa acgctagcaa 960

catggtgtcg gtcggtcggt cggttgttgt aggacatgtt ccatagaaaa agcatagacg 1020

agtctacagg ttttggagcc ttggtttggt cctctgtgta ttcacctttc tgtacaatct 1080

tagtagcgtt gtgtaccttc ccctggaagg aaggatagct tcagttagcg cttcagaaag 1140

tcaagtgtgt agcatattgg cttattgttt gctttgcttg gacaatggag atttgggagt 1200

ggagttcata accctgctga ataaatactc ttagctggct aaaaaaaaaa aaaaaaaa 1258



18
214
PRT
Zea mays

18

Met Thr Ser Val Val His Ser Val Ser Gly Asp His Arg Ala Glu Asp
1 5 10 15

Gln Asn Gln Gln Lys Lys Gln Ala Glu Pro Gly Asp Gln Gln Glu Ala
20 25 30

Pro Val Thr Ser Ser Asp Ser Gln Pro Thr Val Gly Thr Pro Ser Thr
35 40 45

Asp Tyr Val Ala Pro Tyr Ala Pro His Asp Met Ser His Ala Met Gly
50 55 60

Gln Tyr Ala Tyr Pro Asn Ile Asp Pro Tyr Tyr Gly Ser Leu Tyr Ala
65 70 75 80

Ala Ala Tyr Gly Gly Gln Pro Leu Met His Pro Pro Leu Val Gly Met
85 90 95

His Pro Ala Gly Leu Pro Leu Pro Thr Asp Ala Ile Glu Glu Pro Val
100 105 110

Tyr Val Asn Ala Lys Gln Tyr Asn Ala Ile Leu Arg Arg Arg Gln Ser
115 120 125

Arg Ala Lys Ala Glu Ser Glu Arg Lys Leu Ile Lys Gly Arg Lys Pro
130 135 140

Tyr Leu His Glu Ser Arg His Gln His Ala Leu Lys Arg Ala Arg Gly
145 150 155 160

Ala Gly Gly Arg Phe Leu Asn Ser Lys Ser Asp Asp Lys Glu Glu Asn
165 170 175

Ser Asp Ser Ser His Lys Glu Asn Gln Asn Gly Val Ala Pro His Arg
180 185 190

Ser Gly Gln Pro Ser Thr Pro Pro Ser Pro Asn Gly Ala Ser Ser Ala
195 200 205

Asn Gln Gly Arg Gln Ser
210



19
1170
DNA
Zea mays

19

gcacgagcca cgccgtcggc cacgccccga cgaccaacac ctgctccctc cgccgccgcc 60

cgtgtcctcc cgctccgtcc gcgcgccgcc ctcatacctc caagcgcggt tggatctgct 120

ctgggtccaa gtccgctcga tcctcctctc gtcggaaact ttatgtgtgc cttcatccac 180

gaagagctga agatatcaca tgactagttg cagttagtgt ggcttttctc cctgcttggt 240

cctgattgtg tgctttgcct tgcaaaggaa ggaatcatga cctctgttgt tcagagcgtt 300

tcaggtgacc acagggctga ggatcaaagt catcagaaga agcaaactga acctggggac 360

cagcaagaag ccccagttac tagttcagat agccaaccaa cagtgggcac accatcaaca 420

gattatgtgg caccctatgc ccctcatgac atgagccatg caatgggtca atatgcttat 480

ccaaatattg atccatacta tggaagtctt tatgcggcgg cttatggtgg acatccattg 540

atgcatccaa cattagtcgg aatgcatccg gctggcttac ctttgcctac cgatgcaatt 600

gaagagccag tgtatgtaaa tgcaaagcaa tacaatgcca tattaagacg gcgtcaatct 660

cgggctaaag ctgaatcaga acggaagctt gtcaagggcc gcaagcccta tctccatgag 720

tcacggcatc agcatgcctt gaaaagggcc aggggagctg gaggtcggtt tctcaattcg 780

aagtcagatg acaaggaaga gaactccgac tcaagtcaaa aagagattca gaacggagtt 840

gcgccccaaa agggtggcca accgtcaacc cctccgtctc ccaacggtgc gtcgtcagct 900

tatcaggcgc ctagtcgtga atgatgattc ggaactcaca actgaagaga ttttagtccc 960

tgacgctagt tgtggcagca gctttgtaca gtaagtgcta gcgggcagca gcgaaatggt 1020

gtcatagaaa aacgttgacg agtcagacag gttttggagt cttggttttt tttcctctgt 1080

ttattttacc tgtctgcaat tttagtagct ttgtgtccct tcccctggat agttttttgg 1140

tcagcgctta agaaaaaaaa aaaaaaaaaa 1170



20
215
PRT
Zea mays

20

Met Thr Ser Val Val Gln Ser Val Ser Gly Asp His Arg Ala Glu Asp
1 5 10 15

Gln Ser His Gln Lys Lys Gln Thr Glu Pro Gly Asp Gln Gln Glu Ala
20 25 30

Pro Val Thr Ser Ser Asp Ser Gln Pro Thr Val Gly Thr Pro Ser Thr
35 40 45

Asp Tyr Val Ala Pro Tyr Ala Pro His Asp Met Ser His Ala Met Gly
50 55 60

Gln Tyr Ala Tyr Pro Asn Ile Asp Pro Tyr Tyr Gly Ser Leu Tyr Ala
65 70 75 80

Ala Ala Tyr Gly Gly His Pro Leu Met His Pro Thr Leu Val Gly Met
85 90 95

His Pro Ala Gly Leu Pro Leu Pro Thr Asp Ala Ile Glu Glu Pro Val
100 105 110

Tyr Val Asn Ala Lys Gln Tyr Asn Ala Ile Leu Arg Arg Arg Gln Ser
115 120 125

Arg Ala Lys Ala Glu Ser Glu Arg Lys Leu Val Lys Gly Arg Lys Pro
130 135 140

Tyr Leu His Glu Ser Arg His Gln His Ala Leu Lys Arg Ala Arg Gly
145 150 155 160

Ala Gly Gly Arg Phe Leu Asn Ser Lys Ser Asp Asp Lys Glu Glu Asn
165 170 175

Ser Asp Ser Ser Gln Lys Glu Ile Gln Asn Gly Val Ala Pro Gln Lys
180 185 190

Gly Gly Gln Pro Ser Thr Pro Pro Ser Pro Asn Gly Ala Ser Ser Ala
195 200 205

Tyr Gln Ala Pro Ser Arg Glu
210 215



21
1892
DNA
Zea mays

21

ccacgcgtcc gcccgctggg gctgggctac ctcgttcgct tcgctgcctc tgcctactcc 60

tctctcccct ctttctccgc tcatgtgctg gtccatcgtc tgcctcctcg gtttgtcctg 120

aatccttgga cagacgcaca caggctcagc tcaggcggtt gctggatcct ttggcgttcc 180

ccatccggcc aagaatcctg caagagcctg cttggagttg gagccggcca aacctgctgc 240

cgtcgacgtc tcgggcgagg cagccttgag catcagtctc cttgacgagg caagcaggcc 300

atgatgagct tcaagggaca cgaggggttc ggtcaggtgt ccggagccgg gatgagccag 360

gcctcccatg gcgccgcgcc tgccggagcc ccgctgccgt ggtgggctgg ggcccagctg 420

ctgtccggcg agccggcgcc cctgtccccg gaggaggcgc cccgggacac ccagttccag 480

gtcgtgccgg gggcctctca gggcacgccg gatccagcgc cgcccaaggg agggacacct 540

aaggtcctca agttctctgt gttccaaggg aatttggagt cgggtggtaa aggagagaaa 600

accccaaaga actctaccgc tgtcgttctg cagtcgccat tcgcggaata caatggtcgt 660

ttcgagatcg gtctcggtca atctatgctg gtcccttcca gttattcttg tgctgaccag 720

tgctatggca tgcttacgac ttatggaatg agatccatgt ctggtgggag aatgctgttg 780

ccactaattg cgccagccga tgcacccgtt tatgtgaacc cgaaacagta cgaaggcatc 840

ctccgtcgtc gccgtgctcg cgctaaggcg gagagcgaga acaggctcac caaaggcaga 900

aagccttatc tccatgagtc gcgccacctc cacgcgatgc gccgggtgag aggctccggc 960

gggcgcttcc tcaacacgaa taaaggaggg cacggcacgg acgttgctgc aaacgggggc 1020

agcaagatgg cggcggcggc ggcaccatcc cgtctcgcca tgccccctag cgctgagcct 1080

ccatggctgt cagggctcag cgacggcagc aacccgtgct gccactcccg gagtagtgtc 1140

tccagcttgt ccgggtccta cgtggcgagc atctacggtg gcttggagca gcacctccgg 1200

gcgccgccct tcttcacccc gctgccgccc gtcatggacg gcgaccacgg cggccccacg 1260

gccgccacca tctcctcctt caagtgggcg gccagcgacg gctgctgcga gctcctcagg 1320

gcgtgaaccg aggagggagg ggatggctac tcagacgaac ggccttctcc ccgatggctg 1380

gttgtctgta ggcaaatcat tcttggctgt tctgcattgg ggtgcgacct acacatcatc 1440

cgcctaccgt acctacccca cccgtgtccc tgaaattcca gggtgcttgg gttacttaca 1500

ggggtcttgt gtggtgatgt ggctccccca tatgcatttg ctgtaacata gcgtacccaa 1560

accactgttg cttggtactt ctcgctatca ctgcctcatc agtatggatt ctgcatttct 1620

gcgttgtcac agtgtatgaa taattgaggc gtcagacttc agggttgctc cagttcttgg 1680

agataggtct gggtttgttt gaagcttgcc tggaggtctg aaactttgtg tttggtgaag 1740

atgctacgtt attgcagttt gaatctgtaa gtttgggatc agcattcagt tgttgcatcg 1800

tctgtgctct ggtgccgagg tgttcgttct gaatatttga ttcaattcaa aatcttcagc 1860

taagttacta ctgggacaaa aaaaaaaaaa aa 1892



22
341
PRT
Zea mays

22

Met Met Ser Phe Lys Gly His Glu Gly Phe Gly Gln Val Ser Gly Ala
1 5 10 15

Gly Met Ser Gln Ala Ser His Gly Ala Ala Pro Ala Gly Ala Pro Leu
20 25 30

Pro Trp Trp Ala Gly Ala Gln Leu Leu Ser Gly Glu Pro Ala Pro Leu
35 40 45

Ser Pro Glu Glu Ala Pro Arg Asp Thr Gln Phe Gln Val Val Pro Gly
50 55 60

Ala Ser Gln Gly Thr Pro Asp Pro Ala Pro Pro Lys Gly Gly Thr Pro
65 70 75 80

Lys Val Leu Lys Phe Ser Val Phe Gln Gly Asn Leu Glu Ser Gly Gly
85 90 95

Lys Gly Glu Lys Thr Pro Lys Asn Ser Thr Ala Val Val Leu Gln Ser
100 105 110

Pro Phe Ala Glu Tyr Asn Gly Arg Phe Glu Ile Gly Leu Gly Gln Ser
115 120 125

Met Leu Val Pro Ser Ser Tyr Ser Cys Ala Asp Gln Cys Tyr Gly Met
130 135 140

Leu Thr Thr Tyr Gly Met Arg Ser Met Ser Gly Gly Arg Met Leu Leu
145 150 155 160

Pro Leu Ile Ala Pro Ala Asp Ala Pro Val Tyr Val Asn Pro Lys Gln
165 170 175

Tyr Glu Gly Ile Leu Arg Arg Arg Arg Ala Arg Ala Lys Ala Glu Ser
180 185 190

Glu Asn Arg Leu Thr Lys Gly Arg Lys Pro Tyr Leu His Glu Ser Arg
195 200 205

His Leu His Ala Met Arg Arg Val Arg Gly Ser Gly Gly Arg Phe Leu
210 215 220

Asn Thr Asn Lys Gly Gly His Gly Thr Asp Val Ala Ala Asn Gly Gly
225 230 235 240

Ser Lys Met Ala Ala Ala Ala Ala Pro Ser Arg Leu Ala Met Pro Pro
245 250 255

Ser Ala Glu Pro Pro Trp Leu Ser Gly Leu Ser Asp Gly Ser Asn Pro
260 265 270

Cys Cys His Ser Arg Ser Ser Val Ser Ser Leu Ser Gly Ser Tyr Val
275 280 285

Ala Ser Ile Tyr Gly Gly Leu Glu Gln His Leu Arg Ala Pro Pro Phe
290 295 300

Phe Thr Pro Leu Pro Pro Val Met Asp Gly Asp His Gly Gly Pro Thr
305 310 315 320

Ala Ala Thr Ile Ser Ser Phe Lys Trp Ala Ala Ser Asp Gly Cys Cys
325 330 335

Glu Leu Leu Arg Ala
340



23
323
DNA
Zea mays

unsure
(201)
n = A, C, G, or T


23

acgccatcat gcgtcggcgc tgtgcccgtg ccaaagcaga gagggaaaat aggctggtca 60

aaggcaggaa gccatatctc catgagtcac gccatcagca tgcactgcgt cgcccgcgag 120

gctctggcgg acgcttcctg aacacaaaga aagaatccag cgggaaggat gctggtggtg 180

gcagcaaggc aatgtttcaa ncaaccccct catgcgccag gtggcgttct cccaagctcc 240

aaanatccac cagtccagac ctgggccaac cccgancanc gttttccacc tgttcnggtt 300

tccaaagttt tttcaacctn ttt 323



24
77
PRT
Zea mays

UNSURE
(67)
Xaa = any amino acid


24

Ala Ile Met Arg Arg Arg Cys Ala Arg Ala Lys Ala Glu Arg Glu Asn
1 5 10 15

Arg Leu Val Lys Gly Arg Lys Pro Tyr Leu His Glu Ser Arg His Gln
20 25 30

His Ala Leu Arg Arg Pro Arg Gly Ser Gly Gly Arg Phe Leu Asn Thr
35 40 45

Lys Lys Glu Ser Ser Gly Lys Asp Ala Gly Gly Gly Ser Lys Ala Met
50 55 60

Phe Gln Xaa Thr Pro Ser Cys Ala Arg Trp Arg Ser Pro
65 70 75



25
1195
DNA
Zea mays

25

gcaccagacc agaggaaggg acggcgggga ggtggcaagg cgcagagagc aggttcgctt 60

ggcggacgca ccgagggagg cgtgtgggag ccatgcttct tccgtcttcg tcttcgtctt 120

ccgcttccgc ttccgcttcc aaaggtaact cctttgggaa aaccgttaac gatcatctga 180

ggtcaacttt gagttttgat aacaagcaac ctccatttgc aagtcaaaac tttgactacg 240

gtcaaacaat agcttgcatt tcatacccgt acaatcattc tggctcagga gatgtctggg 300

cagcctatga gtcacgcacc agcgctgcca ctgtgttccg ttcccaaatt gctggtgggg 360

gtacatccac aagaattccc ttgcctttgg aattagcaga gaatgaaccc atatatgtga 420

atcccaaaca atatcacggg atacttcgca gaagacagtt acgtgccaag ttagaggctc 480

agaacaagct agtcagagcc cgaaagcctt accttcatga gtctaggcat cttcatgcaa 540

tgaagagggc acgaggttcc ggtggacgat tcctcaacac taagcagctc cagcagtctc 600

acactgccct caccaggtcc accaccacaa gtggcacaag ctcctcaggc tcaactcatc 660

tgcggcttgg tggtggcgca gccgcagctg gagatcgatc tgtgctggca cccaaaacaa 720

tggcctcaca agacagtagc aagaaggccg tttcttcagc cctcgccttc actgcgactc 780

caatgctgcg cagagatgac ggcttcttgc agcacccaag ccatcttttc agtttttctg 840

gtcattttgg gcaggcaagc gcgcaagctg gcgtccataa tggaagtcag catagggttc 900

cagttatgag atgaccggtt tgcgaaccat agctggtgat ccaggcgtct agggtcaact 960

tcgctgtggt gtcttagtct ctcaggcaat tcatccttgg cttaatttct ggctttttat 1020

tagaaggtac caaaatgtgt tccataccgt tgtggccaca gagcccataa accagggggt 1080

ttgatggttg gcactcctac ccaaactatt gtcttgttgc agtggtgttt gttagaataa 1140

accttgacta ttattctgta caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 1195



26
273
PRT
Zea mays

26

Met Leu Leu Pro Ser Ser Ser Ser Ser Ser Ala Ser Ala Ser Ala Ser
1 5 10 15

Lys Gly Asn Ser Phe Gly Lys Thr Val Asn Asp His Leu Arg Ser Thr
20 25 30

Leu Ser Phe Asp Asn Lys Gln Pro Pro Phe Ala Ser Gln Asn Phe Asp
35 40 45

Tyr Gly Gln Thr Ile Ala Cys Ile Ser Tyr Pro Tyr Asn His Ser Gly
50 55 60

Ser Gly Asp Val Trp Ala Ala Tyr Glu Ser Arg Thr Ser Ala Ala Thr
65 70 75 80

Val Phe Arg Ser Gln Ile Ala Gly Gly Gly Thr Ser Thr Arg Ile Pro
85 90 95

Leu Pro Leu Glu Leu Ala Glu Asn Glu Pro Ile Tyr Val Asn Pro Lys
100 105 110

Gln Tyr His Gly Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu Glu
115 120 125

Ala Gln Asn Lys Leu Val Arg Ala Arg Lys Pro Tyr Leu His Glu Ser
130 135 140

Arg His Leu His Ala Met Lys Arg Ala Arg Gly Ser Gly Gly Arg Phe
145 150 155 160

Leu Asn Thr Lys Gln Leu Gln Gln Ser His Thr Ala Leu Thr Arg Ser
165 170 175

Thr Thr Thr Ser Gly Thr Ser Ser Ser Gly Ser Thr His Leu Arg Leu
180 185 190

Gly Gly Gly Ala Ala Ala Ala Gly Asp Arg Ser Val Leu Ala Pro Lys
195 200 205

Thr Met Ala Ser Gln Asp Ser Ser Lys Lys Ala Val Ser Ser Ala Leu
210 215 220

Ala Phe Thr Ala Thr Pro Met Leu Arg Arg Asp Asp Gly Phe Leu Gln
225 230 235 240

His Pro Ser His Leu Phe Ser Phe Ser Gly His Phe Gly Gln Ala Ser
245 250 255

Ala Gln Ala Gly Val His Asn Gly Ser Gln His Arg Val Pro Val Met
260 265 270

Arg



27
1376
DNA
Zea mays

27

tctctatcta tctatacggt tcaagggact gaagaaggta gagagagaaa ctcgaagggg 60

agaggacaga agagggagat acaggttaat ttttaggtac cagatcatct gatttctcag 120

aagcaaaatg ttgtttggag ctcagtgaca ccatcttgta atgcctgtga ttttacggga 180

aatggaggat cattctgtcc atcccatgtc taagtctaac catggctcct tgtcaggaaa 240

tggttatgag atgaaacatt caggccataa agtttgcgat agggattcat catcggagtc 300

tgatcggtct caccaagaag catcagcagc aagtgaaagc agtccaaatg aacacacatc 360

aactcaatca gacaatgatg aagatcatgg gaaagataat caggacacaa tgaagccagt 420

attgtccttg gggaaggaag gctctgcctt tttggcccca aaattacatt acagcccatc 480

ttttgcttgt attccttata ctgctgatgc ttattatagt gcggttgggg tcttgacagg 540

atatcctcca catgccattg tccatcccca gcaaaatgat acaacgaaca ctccgggtat 600

gttacctgtg gaacctgcag aagaaccaat atatgttaat gcaaaacaat accatgcaat 660

ccttaggagg aggcaaacac gtgctaaatt ggaggcccag aacaagatgg tgaaaaatcg 720

gaagccatat cttcatgagt cccgacatcg tcatgccatg aaacgggctc gtggatcagg 780

aggacggttc ctcaacacaa agcagctcca ggagcagaac cagcagtatc aggcatcgag 840

tggttcattg tgctcaaaga tcattgccaa cagcataatc tcccaaagtg gccccacctg 900

cacgccctct tctggcactg caggtgcttc aacagccggc caggaccgca gctgcttgcc 960

ctcagttggc ttccgcccca cgacaaactt cagtgaccaa ggtcgaggag gcttgaagct 1020

ggccgtgatc ggcatgcagc agcgtgtttc caccataagg tgaagagaag tgggcacaac 1080

accattccca ggcacactgc ctgtggcaac tcatccttgg ctcttggaac tttgaatatg 1140

caatcgacat gtagcttgag atcctcagaa taaaccaaac cttcagttat atgcaagcct 1200

tttttgaggt tgctgttgct gtacctgaga actgtggtta ggttatgagt ttgttcctca 1260

aaactgaccc atacatgaca tgctaccttg tgctgagttt ctgagacaaa gccatcgaaa 1320

catgatcttg tggttcagta aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 1376



28
300
PRT
Zea mays

28

Met Pro Val Ile Leu Arg Glu Met Glu Asp His Ser Val His Pro Met
1 5 10 15

Ser Lys Ser Asn His Gly Ser Leu Ser Gly Asn Gly Tyr Glu Met Lys
20 25 30

His Ser Gly His Lys Val Cys Asp Arg Asp Ser Ser Ser Glu Ser Asp
35 40 45

Arg Ser His Gln Glu Ala Ser Ala Ala Ser Glu Ser Ser Pro Asn Glu
50 55 60

His Thr Ser Thr Gln Ser Asp Asn Asp Glu Asp His Gly Lys Asp Asn
65 70 75 80

Gln Asp Thr Met Lys Pro Val Leu Ser Leu Gly Lys Glu Gly Ser Ala
85 90 95

Phe Leu Ala Pro Lys Leu His Tyr Ser Pro Ser Phe Ala Cys Ile Pro
100 105 110

Tyr Thr Ala Asp Ala Tyr Tyr Ser Ala Val Gly Val Leu Thr Gly Tyr
115 120 125

Pro Pro His Ala Ile Val His Pro Gln Gln Asn Asp Thr Thr Asn Thr
130 135 140

Pro Gly Met Leu Pro Val Glu Pro Ala Glu Glu Pro Ile Tyr Val Asn
145 150 155 160

Ala Lys Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Thr Arg Ala Lys
165 170 175

Leu Glu Ala Gln Asn Lys Met Val Lys Asn Arg Lys Pro Tyr Leu His
180 185 190

Glu Ser Arg His Arg His Ala Met Lys Arg Ala Arg Gly Ser Gly Gly
195 200 205

Arg Phe Leu Asn Thr Lys Gln Leu Gln Glu Gln Asn Gln Gln Tyr Gln
210 215 220

Ala Ser Ser Gly Ser Leu Cys Ser Lys Ile Ile Ala Asn Ser Ile Ile
225 230 235 240

Ser Gln Ser Gly Pro Thr Cys Thr Pro Ser Ser Gly Thr Ala Gly Ala
245 250 255

Ser Thr Ala Gly Gln Asp Arg Ser Cys Leu Pro Ser Val Gly Phe Arg
260 265 270

Pro Thr Thr Asn Phe Ser Asp Gln Gly Arg Gly Gly Leu Lys Leu Ala
275 280 285

Val Ile Gly Met Gln Gln Arg Val Ser Thr Ile Arg
290 295 300



29
1492
DNA
Zea mays

29

gcacgagctc acttgcttcg acgtatttct caatctatct atacggttca agggaccgaa 60

gaaggtagag agagaaactt gaaggggaga ggaaggagat acaggttcat gttcatttag 120

gtgtcagttc atctgatttc tcagaagcaa aatgttgttt ggagctcagt gacaccatct 180

tgtaatgcat gtgcctttta cgggaaatgg aggatcattc tgtccatcca aagtctaagt 240

ctaaccatgg ttccttgtca ggaaatggtt atgagatgaa aaatccaggc catgaagttt 300

gtgataggga ttcatcatca gagtctgatc gatctcaccc agaagcatca gcagtgagtg 360

aaagcagtct agatgaacac acatcaactc aatcagacaa tgatgaagat catgggaagg 420

ataatcagga cacattgaag ccagtattgt ccttggggaa ggaagggtct gcctttttgg 480

ccccaaaaat agattacaac ccgtcttttc cttatattcc ttatactgct gacgcttact 540

atggtggcgt tggggtcttg acaggatatg ctccgcatgc cattgtccat ccccagcaaa 600

atgatacaac aaatagtccg gttatgttgc ctgcggaacc tgcagaagaa gaaccaatat 660

atgtcaatgc aaaacaatac catgcaatcc ttaggaggag gcagacacgt gctaaactgg 720

aggcgcagaa caagatggtg aaaggtcgga agccatacct tcatgagtct cgacaccgtc 780

atgccatgaa gcgggcccgt ggctcaggag ggcggttcct caacacaaag cagcagctcc 840

aggagcagaa ccagcggtac caggcgtcga gtggttcaat gtgctcaaag accattggca 900

acagcgtaat ctcccaaagt ggccccattt gcacgccctc ttctgacgct gcaggtgctt 960

cagcagccag ccaggaccgc ggctgcttgc cctcggttgg cttccgcccc acagccaact 1020

tcagtgagca aggtggaggc ggctcgaagc tggtcatgaa cggcatgcag cagcgtgttt 1080

ccaccataag gtgaagagaa gtgggcacga caccattccc aggcgcgcac tgcctgtggc 1140

aactcatcct tggcttttga aactatggat atgcaatgga catgtagctt cgagttcctc 1200

agaataacca aacgtgaaga atatgcaaag tccttttgag atttgctgta gctgaaagaa 1260

ctgtggttag gttgagtttc ttcctggaga ctgatccata catgacatgc tacctcgtgc 1320

tgagtttctg aggtgaagcc atcgaaacat gaccgtgtgg ttcagtaaaa aaaaaaaaaa 1380

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1440

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 1492



30
301
PRT
Zea mays

30

Met Cys Leu Leu Arg Glu Met Glu Asp His Ser Val His Pro Lys Ser
1 5 10 15

Lys Ser Asn His Gly Ser Leu Ser Gly Asn Gly Tyr Glu Met Lys Asn
20 25 30

Pro Gly His Glu Val Cys Asp Arg Asp Ser Ser Ser Glu Ser Asp Arg
35 40 45

Ser His Pro Glu Ala Ser Ala Val Ser Glu Ser Ser Leu Asp Glu His
50 55 60

Thr Ser Thr Gln Ser Asp Asn Asp Glu Asp His Gly Lys Asp Asn Gln
65 70 75 80

Asp Thr Leu Lys Pro Val Leu Ser Leu Gly Lys Glu Gly Ser Ala Phe
85 90 95

Leu Ala Pro Lys Ile Asp Tyr Asn Pro Ser Phe Pro Tyr Ile Pro Tyr
100 105 110

Thr Ala Asp Ala Tyr Tyr Gly Gly Val Gly Val Leu Thr Gly Tyr Ala
115 120 125

Pro His Ala Ile Val His Pro Gln Gln Asn Asp Thr Thr Asn Ser Pro
130 135 140

Val Met Leu Pro Ala Glu Pro Ala Glu Glu Glu Pro Ile Tyr Val Asn
145 150 155 160

Ala Lys Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Thr Arg Ala Lys
165 170 175

Leu Glu Ala Gln Asn Lys Met Val Lys Gly Arg Lys Pro Tyr Leu His
180 185 190

Glu Ser Arg His Arg His Ala Met Lys Arg Ala Arg Gly Ser Gly Gly
195 200 205

Arg Phe Leu Asn Thr Lys Gln Gln Leu Gln Glu Gln Asn Gln Arg Tyr
210 215 220

Gln Ala Ser Ser Gly Ser Met Cys Ser Lys Thr Ile Gly Asn Ser Val
225 230 235 240

Ile Ser Gln Ser Gly Pro Ile Cys Thr Pro Ser Ser Asp Ala Ala Gly
245 250 255

Ala Ser Ala Ala Ser Gln Asp Arg Gly Cys Leu Pro Ser Val Gly Phe
260 265 270

Arg Pro Thr Ala Asn Phe Ser Glu Gln Gly Gly Gly Gly Ser Lys Leu
275 280 285

Val Met Asn Gly Met Gln Gln Arg Val Ser Thr Ile Arg
290 295 300



31
725
DNA
Zea mays

unsure
(546)
n = A, C, G, or T


31

gcagcaaaca ctagggtacc attgccagtt gggcctgcag cagaggaacc catatttgtc 60

aatgcaaagc aatacaatgc tatcctccgg aggaggcaaa aacgcgcaaa actggaggcc 120

caaaataaac tggtgaaagg tcggaagcca tatctccatg aatctcggca tcgtcatgca 180

atgaagcgag tccgtggacc agggcgtttc ctcaacaaaa aggagctcca ggagcagcag 240

ctgaaggcac tgccttcact tcagactcca acaggtgggg tcagcaaaat ggcctttggc 300

aggaacctat gccctgaaag cagcacatct cactcgcctt cgacgagctc tacaatctcg 360

agtgcttcaa actggagtgg cacgctagct catcaagagc acgttagctt cgcatctgct 420

aataaattcc tccccagcat gaacttccac gcggagaatg gagtgaaaag atggccatca 480

atggcgtccg ccaccacacc cctgtcctga gtgaacaacc ttcaactgtg ggggtgctgt 540

gctggnacca tcantgggcg cgctccgtgt gcccgtggca attcatcttg gcttatgatg 600

tatcttatag ttaatttgct ttcactttca tatggnactt gtctcagatt aaactcgtga 660

tatttattgc nactgggatg actggaaata atctcangtt tcttaccaaa aaaaaaaaaa 720

aaaaa 725



32
169
PRT
Zea mays

32

Ala Ala Asn Thr Arg Val Pro Leu Pro Val Gly Pro Ala Ala Glu Glu
1 5 10 15

Pro Ile Phe Val Asn Ala Lys Gln Tyr Asn Ala Ile Leu Arg Arg Arg
20 25 30

Gln Lys Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Val Lys Gly Arg
35 40 45

Lys Pro Tyr Leu His Glu Ser Arg His Arg His Ala Met Lys Arg Val
50 55 60

Arg Gly Pro Gly Arg Phe Leu Asn Lys Lys Glu Leu Gln Glu Gln Gln
65 70 75 80

Leu Lys Ala Leu Pro Ser Leu Gln Thr Pro Thr Gly Gly Val Ser Lys
85 90 95

Met Ala Phe Gly Arg Asn Leu Cys Pro Glu Ser Ser Thr Ser His Ser
100 105 110

Pro Ser Thr Ser Ser Thr Ile Ser Ser Ala Ser Asn Trp Ser Gly Thr
115 120 125

Leu Ala His Gln Glu His Val Ser Phe Ala Ser Ala Asn Lys Phe Leu
130 135 140

Pro Ser Met Asn Phe His Ala Glu Asn Gly Val Lys Arg Trp Pro Ser
145 150 155 160

Met Ala Ser Ala Thr Thr Pro Leu Ser
165



33
831
DNA
Zea mays

33

ccacgcgtcc gcatatatgt gaatcccaaa caatatcacg ggatacttcg cagaagacag 60

ttacgtgcca agctagaggc tcagaacaag ctagtcagag cccgaaagtc ttaccttcat 120

gagtctaggc atcttcatgc aatgaagagg gcacgaggtt ccggtggacg attcctcaac 180

actaagcagc tccagcagtc tcacacagcc ctcaccaggt ccaccaccac aagtggcaca 240

agctcctcag gctcaactca tctgcggctt ggtggtggcg cagccgcagc tggagatcga 300

tctgtgctgg cacccaaaac aatggcctca caagacagta gcaagaaggc cgtttcttca 360

gccctcgcct tcactgcgac tccaatgctg cgcagagatg acggcttctt gcagcaccca 420

agccatcttt tcagtttttc tggtcatttt gggcaggcaa gcgcgcaagc tggcgtccat 480

aatggaagtc agcatagggt tccagttatg agatgaccgg tttgcgaacc atagctggtg 540

atccaggcgt ctagggtcaa cttcgctgtg gtgtcttagt ctctcaggca attcatcctt 600

ggcttaattt ctggcttttt attagaaggt accaaaatgt gttccatacc gttgtggcca 660

cagagcccat aaaccagggg gtttgatggt tggcactcct acccaaacta ttgttgcagt 720

ggtgtttgtt agaataaacc ttgactatta ttctgtacaa tttgccttta tcttgtactg 780

ccaattattg tgtagtggtc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa g 831



34
98
PRT
Zea mays

34

Ile Tyr Val Asn Pro Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln
1 5 10 15

Leu Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Val Arg Ala Arg Lys
20 25 30

Ser Tyr Leu His Glu Ser Arg His Leu His Ala Met Lys Arg Ala Arg
35 40 45

Gly Ser Gly Gly Arg Phe Leu Asn Thr Lys Gln Leu Gln Gln Ser His
50 55 60

Thr Ala Leu Thr Arg Ser Thr Thr Thr Ser Gly Thr Ser Ser Ser Gly
65 70 75 80

Ser Thr His Leu Arg Leu Gly Gly Gly Ala Ala Ala Ala Gly Asp Arg
85 90 95

Ser Val



35
1307
DNA
Zea mays

35

ccacgcgtcc gctgtctgtg tgcgagcgca agagaaaggg agtcagagag agagagagag 60

ggaggagacc ttgcagagga gcgaagcaag caaggtggga aagaggcagc agcaagggcg 120

gcgggctgcc ggaaggggaa catgctccct cctcatctca cagtacgaac tgaaaaacaa 180

gagtaaagaa tttccgtgag atgagacaga atggcgcggt gatgattcag tttggccatc 240

agatgcctga ttacgactcc ccggctaccc agtcaaccag tgagacgagc catcaagaag 300

cgtctggaat gagcgaaggg agcctcaacg agcataataa tgaccattca ggcaaccttg 360

atgggtactc gaagagtgac gaaaacaaga tgatgtcagc gttatccctg ggcaatccgg 420

aaacagctta cgcacataat ccgaagcctg accgtactca gtccttcgcc atatcatacc 480

catatgccga tccatactac ggtggcgcgg tggcagcagc ttatggcccg catgctatca 540

tgcaccctca gctggttggc atggttccgt cctctcgagt gccactgccg atcgagccag 600

ccgctgaaga gcccatctat gtcaacgcga agcagtacca cgctattctc cggaggagac 660

agctccgtgc aaagctagag gcggaaaaca agctcgtgaa aagccgcaag ccgtacctcc 720

acgagtctcg gcacctgcac gcgatgaaga gagctcgggg aacaggcggg cggttcctga 780

acacgaagca gcagccggag tcccccggca gcggcggctc ctcggacgcg caacgcgtgc 840

ccgcgaccgc gagcggcggc ctgttcacga agcatgagca cagcctgccg cccggcggtc 900

gccaccacta tcacgcgaga gggggcggtg agtagggagc cccgacactg gcaactcatc 960

cttggcttat cagcgattcg actcggctct ccctcgtctg aaactgaact ctctgcaact 1020

actgtaactg taactaaact gggtgtgccc ggattggcgg tcgttctgtt ctactactag 1080

tacctgctac gcgtcgttgg gttgggtctg gactagagag cgtgctggtt ctttgatgaa 1140

cttggctgga cttgagggtg ttgactagcg cgaagctgag ttccatgtaa aacttttgct 1200

tcaagaccga tgactggcgg cataataagt agcagtaata cccaaaaaaa aaaaaaaaaa 1260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaag 1307



36
244
PRT
Zea mays

36

Met Arg Gln Asn Gly Ala Val Met Ile Gln Phe Gly His Gln Met Pro
1 5 10 15

Asp Tyr Asp Ser Pro Ala Thr Gln Ser Thr Ser Glu Thr Ser His Gln
20 25 30

Glu Ala Ser Gly Met Ser Glu Gly Ser Leu Asn Glu His Asn Asn Asp
35 40 45

His Ser Gly Asn Leu Asp Gly Tyr Ser Lys Ser Asp Glu Asn Lys Met
50 55 60

Met Ser Ala Leu Ser Leu Gly Asn Pro Glu Thr Ala Tyr Ala His Asn
65 70 75 80

Pro Lys Pro Asp Arg Thr Gln Ser Phe Ala Ile Ser Tyr Pro Tyr Ala
85 90 95

Asp Pro Tyr Tyr Gly Gly Ala Val Ala Ala Ala Tyr Gly Pro His Ala
100 105 110

Ile Met His Pro Gln Leu Val Gly Met Val Pro Ser Ser Arg Val Pro
115 120 125

Leu Pro Ile Glu Pro Ala Ala Glu Glu Pro Ile Tyr Val Asn Ala Lys
130 135 140

Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu Glu
145 150 155 160

Ala Glu Asn Lys Leu Val Lys Ser Arg Lys Pro Tyr Leu His Glu Ser
165 170 175

Arg His Leu His Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg Phe
180 185 190

Leu Asn Thr Lys Gln Gln Pro Glu Ser Pro Gly Ser Gly Gly Ser Ser
195 200 205

Asp Ala Gln Arg Val Pro Ala Thr Ala Ser Gly Gly Leu Phe Thr Lys
210 215 220

His Glu His Ser Leu Pro Pro Gly Gly Arg His His Tyr His Ala Arg
225 230 235 240

Gly Gly Gly Glu



37
816
DNA
Zea mays

37

ccacgcgtcc gcgcagaaca agatggtgaa aggccggaag ccataccttc atgagtctcg 60

acaccgtcat gccatgaagc gggcccgtgg ctcaggaggg cggttcctca acacaaagca 120

gcagccccag gagcagaacc agcagtacca ggcgtcgagt ggttcaatgt gctcaaagac 180

cattggcaac agcgtaatct cccaaagtgg ccccatttgc acgccctctt ctgacgctgc 240

aggtgcttca gcagccagcc aggaccgcgg ctgcttgccc tcggtgggct tccgccccac 300

agccaacttc agtgagcaag gtggaggcgg ctcgaagctg gtcgtgaacg gcatgcagca 360

gcgtgtttcc accataaggt gaagagaagt gggcacgaca ccattcccag gcgcgcactg 420

cctgtggcaa ctcatccttg gcttttgaaa ctatggatat gcaatggaca tgtagcttcg 480

agttcctcag aataaccaaa cgtgaagaat atgcaaagtc cttttgagat ttgctgtagc 540

tgaaagaact gtggttaggt tatgagtttc ttcctggaga ctgatccata catgacatgc 600

tacctcgtgc tgagtttctg aggtgaagcc atcgaaacat gaccgtgtgg ttcagtaccc 660

ttgctgcctt cagtgtctga taagctagct ctccagtttg cagtttctct gaattccagc 720

atgtctagtc tctgcttatc ttttgcatgt aacgtgatgg tgacttagca tacacatcta 780

ttcatccatc tatgttctca aaaaaaaaaa aaaaag 816



38
78
PRT
Zea mays

38

His Ala Ser Ala Gln Asn Lys Met Val Lys Gly Arg Lys Pro Tyr Leu
1 5 10 15

His Glu Ser Arg His Arg His Ala Met Lys Arg Ala Arg Gly Ser Gly
20 25 30

Gly Arg Phe Leu Asn Thr Lys Gln Gln Pro Gln Glu Gln Asn Gln Gln
35 40 45

Tyr Gln Ala Ser Ser Gly Ser Met Cys Ser Lys Thr Ile Gly Asn Ser
50 55 60

Val Ile Ser Gln Ser Gly Pro Ile Cys Thr Pro Ser Ser Asp
65 70 75



39
1630
DNA
Argemone mexicana

39

gcacgagtgc agacaagagt agattttatg aaatcgatgg ctctaaaatc tctaaaaagt 60

gagtgttcta gggtttattc ttttactgtt ctcaataaca attggatagg agattgattg 120

tttttgaagt aatttgaacc atgcactcga ttcctgggaa tgtgaatgca acagaatcgg 180

acgtgcaacg tactccgcaa tcaactattt gttctcaacc ttggtggtgt ggtactgtgt 240

ataacactgg ttcgtcagct gagttgggag aaagcacaat aaaatcgtct tcaatggaac 300

agccagacgg tggaatgggt attgatacca gagaatcaca tggtgatggt ggtcctaatg 360

agggggatgg tattacgaga aagatgcaca ccaccatggc ctcccaatct gggccagatg 420

gaaactatgg acatgaacat gggaatctgc agcatgctgc atctgcaatg ccccaaacta 480

gtggtgaata cgtcataccg cgtccacagt ttgagcttgt tggtcactca gttgcatgtg 540

caacgtaccc gtattctgat atgtattata ctggaatgat ggctgctttg ggaactcagg 600

ctcaggtaca tcctcattta tttggtgtac aacacaccag aatgccttta cctcttgaaa 660

tggctgaaga gcctgtctat gtaaatgcga agcaatatca tggaattctg agacgaaggc 720

agtcgcgtgc aaaggctgag ctagaaagga aactgattaa atctagaaag ccgtaccttc 780

atgaatctcg gcaccaacat gctatgagaa gggcaagggg ttgtggaggc cgttttctca 840

acacaaaaaa actcgaaaac gggtcatcta agcatacaac tgagaacagc atggcttctg 900

attgtaatgg taaccggaac tccccaagtg gtcaacaaga aatagaaggt tccaacgtgc 960

aggaatcaca ttcctacttt aacagcaatg ataaaagctg ctaccaacat aatcagggtc 1020

tgcagttatc aagtttccat ccattatctg gtgagagagg agaggaagga gactgttcag 1080

gcctgcagcg aggaagcatc tcggtgaacc aggcccagaa cagggccctc accatccagt 1140

gaacctctga gtaggggaat agggtttctc catcgtcagt atcccgtttg ctgttactgc 1200

tctgggactt caaataccat gtaagcaacg gaaagcagca atggcgctga agggatggac 1260

gcaaaccaga aacggattcc ccccaaggta attggtgttt ctcaggcaat tcattcttgg 1320

cttggttctt gtgtttgatg gggaaagagg agtgtaggtt ctatttggtt ctgtggtgtc 1380

cttacaactt ctctactctt tccctcttgt ttttttttta tcccttgttg tacaaaggaa 1440

atgatagtgg ctgttttaga atctaagtag tgagaagaaa ccaaaccaaa cccttttttc 1500

ttcaaaattt cgtgaaacat tgttttaact ctgtagacat caaaattttc taggcatgta 1560

aaatattcgt cttttttttt ttccatgaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1620

aaaaaaaaaa 1630



40
333
PRT
Argemone mexicana

40

Met His Ser Ile Pro Gly Asn Val Asn Ala Thr Glu Ser Asp Val Gln
1 5 10 15

Arg Thr Pro Gln Ser Thr Ile Cys Ser Gln Pro Trp Trp Cys Gly Thr
20 25 30

Val Tyr Asn Thr Gly Ser Ser Ala Glu Leu Gly Glu Ser Thr Ile Lys
35 40 45

Ser Ser Ser Met Glu Gln Pro Asp Gly Gly Met Gly Ile Asp Thr Arg
50 55 60

Glu Ser His Gly Asp Gly Gly Pro Asn Glu Gly Asp Gly Ile Thr Arg
65 70 75 80

Lys Met His Thr Thr Met Ala Ser Gln Ser Gly Pro Asp Gly Asn Tyr
85 90 95

Gly His Glu His Gly Asn Leu Gln His Ala Ala Ser Ala Met Pro Gln
100 105 110

Thr Ser Gly Glu Tyr Val Ile Pro Arg Pro Gln Phe Glu Leu Val Gly
115 120 125

His Ser Val Ala Cys Ala Thr Tyr Pro Tyr Ser Asp Met Tyr Tyr Thr
130 135 140

Gly Met Met Ala Ala Leu Gly Thr Gln Ala Gln Val His Pro His Leu
145 150 155 160

Phe Gly Val Gln His Thr Arg Met Pro Leu Pro Leu Glu Met Ala Glu
165 170 175

Glu Pro Val Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu Arg Arg
180 185 190

Arg Gln Ser Arg Ala Lys Ala Glu Leu Glu Arg Lys Leu Ile Lys Ser
195 200 205

Arg Lys Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg
210 215 220

Ala Arg Gly Cys Gly Gly Arg Phe Leu Asn Thr Lys Lys Leu Glu Asn
225 230 235 240

Gly Ser Ser Lys His Thr Thr Glu Asn Ser Met Ala Ser Asp Cys Asn
245 250 255

Gly Asn Arg Asn Ser Pro Ser Gly Gln Gln Glu Ile Glu Gly Ser Asn
260 265 270

Val Gln Glu Ser His Ser Tyr Phe Asn Ser Asn Asp Lys Ser Cys Tyr
275 280 285

Gln His Asn Gln Gly Leu Gln Leu Ser Ser Phe His Pro Leu Ser Gly
290 295 300

Glu Arg Gly Glu Glu Gly Asp Cys Ser Gly Leu Gln Arg Gly Ser Ile
305 310 315 320

Ser Val Asn Gln Ala Gln Asn Arg Ala Leu Thr Ile Gln
325 330



41
1565
DNA
Argemone mexicana

41

caagaaagaa aagagagaag aaagaaaatt ttttgaaggt gggtttgaac agaggagaca 60

tgaccagatc tatcccaaca tctcttctcc ttatttctct cactttacca aatcccaaag 120

taaattcact ccagaagcgc gtaatatagg ttttcaaaaa cagttctgag gattttagat 180

tgttttcatc ttggtttgga atttacatag tgaagttaag tgaacaagaa tgcaagacaa 240

gtcaatttca catagtgttg ttagttgtcc aatttggtgg acttctactg gatcccaagt 300

tccacagagt tgtttatcaa agagtttaag cgtaaccttc gactcttctc gtcaagattg 360

cggtagtttg aagcagctag gttttcaact tcaagatcag gattcatcct cgactcaatc 420

aactggtcag tcgcatcatg aagtgggaaa tatgtctgga agcaacccta ctgggcaatg 480

catttcagct cagtgcgaaa aagttactta cgggaaacaa ggagatgttc aaacgaaatc 540

aattctatca cttggagctc cagaagttgt tctccctcaa caagttgatt ataaccacca 600

ctcagtggct cgtataccct atcattacgt tgatccgtat tacggtggca taatggcgtc 660

ttatggacca caggctatta ttcacccaca aatgatgggt ataacacctg cacgagtccc 720

attgcctctt gatcttgcag aaaatgagcc catgtatgtt aatgcaaaac agtaccgagc 780

aattcttaga cggaggcagt cccgtgctaa gcttgaggct caaaataaac ttatcaaaga 840

tcgcaagcct tatctacatg aatctcggca tcttcatgca ttgaagaggg ctaggggatc 900

tggtggacgt tttctcaaca cgaagcagct gcaagagttg aaacaaaaca actctaatgg 960

ccaaaatacc tccgagtcag cttatctaca gttgggagga aatctatctg aatcagaatt 1020

tggcaacggt ggcggtgctt ccaccacatc ctgctctgac atcactacag cctcaaacag 1080

cgaccacatt ttccgtcaac agaatctcag gtttgcgggt tacactcaca tgggtgggac 1140

catgcaagat ggaggtggag ggggcattat gagtaacggg tctcaccacc gtgttcccgt 1200

tacacagtaa aaaacatggg gagaaaaaca actttgtcag ccttttcgat tttggtgtga 1260

agaatggtgt gtactctcag ggtggaactg gagaactggc tggcttgtgt tgtttaccca 1320

tgggcaaatc atccttggct ttgttacctt ttatttatca ctatactttt tatatgatgt 1380

ttcttgctat atatgttttg ttgattttaa cttccataga tggacaatga tgaatttctg 1440

atactggatt gtccttgaaa ctcttcgctt ttattatata ttttgcgaaa aaaaaaaaaa 1500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1560

aaaaa 1565



42
326
PRT
Argemone mexicana

42

Met Gln Asp Lys Ser Ile Ser His Ser Val Val Ser Cys Pro Ile Trp
1 5 10 15

Trp Thr Ser Thr Gly Ser Gln Val Pro Gln Ser Cys Leu Ser Lys Ser
20 25 30

Leu Ser Val Thr Phe Asp Ser Ser Arg Gln Asp Cys Gly Ser Leu Lys
35 40 45

Gln Leu Gly Phe Gln Leu Gln Asp Gln Asp Ser Ser Ser Thr Gln Ser
50 55 60

Thr Gly Gln Ser His His Glu Val Gly Asn Met Ser Gly Ser Asn Pro
65 70 75 80

Thr Gly Gln Cys Ile Ser Ala Gln Cys Glu Lys Val Thr Tyr Gly Lys
85 90 95

Gln Gly Asp Val Gln Thr Lys Ser Ile Leu Ser Leu Gly Ala Pro Glu
100 105 110

Val Val Leu Pro Gln Gln Val Asp Tyr Asn His His Ser Val Ala Arg
115 120 125

Ile Pro Tyr His Tyr Val Asp Pro Tyr Tyr Gly Gly Ile Met Ala Ser
130 135 140

Tyr Gly Pro Gln Ala Ile Ile His Pro Gln Met Met Gly Ile Thr Pro
145 150 155 160

Ala Arg Val Pro Leu Pro Leu Asp Leu Ala Glu Asn Glu Pro Met Tyr
165 170 175

Val Asn Ala Lys Gln Tyr Arg Ala Ile Leu Arg Arg Arg Gln Ser Arg
180 185 190

Ala Lys Leu Glu Ala Gln Asn Lys Leu Ile Lys Asp Arg Lys Pro Tyr
195 200 205

Leu His Glu Ser Arg His Leu His Ala Leu Lys Arg Ala Arg Gly Ser
210 215 220

Gly Gly Arg Phe Leu Asn Thr Lys Gln Leu Gln Glu Leu Lys Gln Asn
225 230 235 240

Asn Ser Asn Gly Gln Asn Thr Ser Glu Ser Ala Tyr Leu Gln Leu Gly
245 250 255

Gly Asn Leu Ser Glu Ser Glu Phe Gly Asn Gly Gly Gly Ala Ser Thr
260 265 270

Thr Ser Cys Ser Asp Ile Thr Thr Ala Ser Asn Ser Asp His Ile Phe
275 280 285

Arg Gln Gln Asn Leu Arg Phe Ala Gly Tyr Thr His Met Gly Gly Thr
290 295 300

Met Gln Asp Gly Gly Gly Gly Gly Ile Met Ser Asn Gly Ser His His
305 310 315 320

Arg Val Pro Val Thr Gln
325



43
1187
DNA
Oryza sativa

43

gcacgaggca gaggagagaa gcaaggtgag aagtgaggag gcagcaaggg aggaggtttg 60

ccggagaggg gacatgctcc ctcctcatct cacagaaaat ggcacagtaa tgattcagtt 120

tggtcataaa atgcctgact acgagtcatc agctacccaa tcaactagtg gatctcctcg 180

tgaagtgtct ggaatgagcg aaggaagcct caatgagcag aatgatcaat ctggtaatct 240

tgatggttac acgaagagtg atgaaggtaa gatgatgtca gctttatctc tgggcaaatc 300

agaaactgtg tatgcacatt cggaacctga ccgtagccaa ccctttggca tatcatatcc 360

atatgctgat tcgttctatg gtggtgctgt agcgacttat ggcacacatg ctattatgca 420

tccccagatt gtgggcgtga tgtcatcctc ccgagtcccg ctaccaatag aaccagccac 480

cgaagagcct atttatgtaa atgcaaagca ataccatgcg attctccgaa ggagacagct 540

ccgtgcaaag ttagaggctg aaaacaagct ggtgaaaaac cgcaagccgt acctccatga 600

atcccggcat caacacgcga tgaagagagc tcggggaaca ggggggagat tcctcaacac 660

aaagcagcag cctgaagctt cagatggtgg caccccaagg ctcgtctctg caaacggcgt 720

tgtgttctca aagcacgagc acagcttgtc gtccagtgat ctccatcatc gtcgtgtgaa 780

agagggcgct tgagatcctc gccgtttctg tcatggcaaa tcatccttgg cttatgtgtg 840

gtgcccagca aaaaaaaatc tgactgaacc tgtgtgtaaa ctgatgggta tgggtgggtt 900

ttgtgcaact gtaactaggg tgcttgacat ctgtgtctgt tgttcctctg cctccttagt 960

ttggagacgg tgcagctgca gctggtacca gtaatctgat catgctagac ttgtgacaag 1020

gacaaaacta gcaccccgtt atgtttcctg gcttctgaat ttggtggtca ttcagtaagc 1080

aagcactcga cgtcagcggg agggggttgc ttcgattgat ctagttcttt cgcgataaac 1140

ttatttaatt ttgaacaaag gttggtttca aaaaaaaaaa aaaaaaa 1187



44
239
PRT
Oryza sativa

44

Met Leu Pro Pro His Leu Thr Glu Asn Gly Thr Val Met Ile Gln Phe
1 5 10 15

Gly His Lys Met Pro Asp Tyr Glu Ser Ser Ala Thr Gln Ser Thr Ser
20 25 30

Gly Ser Pro Arg Glu Val Ser Gly Met Ser Glu Gly Ser Leu Asn Glu
35 40 45

Gln Asn Asp Gln Ser Gly Asn Leu Asp Gly Tyr Thr Lys Ser Asp Glu
50 55 60

Gly Lys Met Met Ser Ala Leu Ser Leu Gly Lys Ser Glu Thr Val Tyr
65 70 75 80

Ala His Ser Glu Pro Asp Arg Ser Gln Pro Phe Gly Ile Ser Tyr Pro
85 90 95

Tyr Ala Asp Ser Phe Tyr Gly Gly Ala Val Ala Thr Tyr Gly Thr His
100 105 110

Ala Ile Met His Pro Gln Ile Val Gly Val Met Ser Ser Ser Arg Val
115 120 125

Pro Leu Pro Ile Glu Pro Ala Thr Glu Glu Pro Ile Tyr Val Asn Ala
130 135 140

Lys Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu
145 150 155 160

Glu Ala Glu Asn Lys Leu Val Lys Asn Arg Lys Pro Tyr Leu His Glu
165 170 175

Ser Arg His Gln His Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg
180 185 190

Phe Leu Asn Thr Lys Gln Gln Pro Glu Ala Ser Asp Gly Gly Thr Pro
195 200 205

Arg Leu Val Ser Ala Asn Gly Val Val Phe Ser Lys His Glu His Ser
210 215 220

Leu Ser Ser Ser Asp Leu His His Arg Arg Val Lys Glu Gly Ala
225 230 235



45
1442
DNA
Oryza sativa

45

gcacgagtac agcgctccgc attagggctc gcctctcgtt ggctagagcg cgagagccag 60

tagccgcagc tgcagcaagc agcagcagca gcgaagagcc tgagccccag aggaggcgtg 120

caccgcctcc gattggccgg cctctcggag agagagagag agagagagat cgatcgagtc 180

ctattggccg ccgcctccgc gccctggctg ctcactggtg agcgagcatg gagtcgaggc 240

cggggggaac caacctcgtg gagccgaggg ggcagggcgc gctgccgtcc ggcataccga 300

tccagcagcc gtggtggacg acctccgccg gggtcggggc ggtgtcgccc gccgtcgtgg 360

cgccggggag cggtgcgggg atcagcctgt cgggcaggga tggcggcggc gacgacgcgg 420

cagaggagag cagcgatgac tcacgaagat caggggagac caaagatgga agcactgatc 480

aagaaaagca tcatgcaaca tcgcagatga ctgctttggc atcagactat ttaacaccat 540

tttcacagct ggaactaaac caaccaattg cttcggcagc ataccagtac cctgactctt 600

actatatggg catggttggt ccctatggac ctcaagctat gtccgcacag actcatttcc 660

agctacctgg attaactcac tctcgtatgc cgttgcctct tgaaatatct gaggagcctg 720

tttatgtaaa tgctaagcaa tatcatggaa ttttaagacg gaggcagtca cgtgcgaagg 780

ctgaacttga gaaaaaagtt gttaaatcaa gaaagcccta tcttcatgag tctcgtcatc 840

aacatgctat gcgaagggca agaggaacgg gtggacgctt cctgaacaca aagaaaaatg 900

aagatggtgc tcccagtgag aaagccgaac caaacaaagg agagcagaac tccgggtatc 960

gccggatccc tcctgactta cagctcctac agaaggaaac atgaagtagc ggctcgaaac 1020

ctagaacagt ggcttctgtc caccggcatt cactcttgag gtgattcttg ctccagaatt 1080

gtgctccatc tttcaaatga tcttcatcga gcaaagtaat tatatgtaca ttcctctgaa 1140

tgatctatgc accaattgtt gatcctggca gggtaataat ctggatgtat tgagtccatc 1200

acagtgcgaa tgtcacgggt agatctgctg ttttcaggca attcattctt ggctttctat 1260

cccacccgtt gttgttgcaa gttaagctag cagtacttgt ctcagtgtcc gtgagacgtt 1320

tgtgtaagat taggttaaac tagaagttgt aatgctgtat taagtgtttg tatttctaat 1380

atgaaccgta acaaggccag agcagaactc gttatacata caaaaaaaaa aaaaaaaaaa 1440

aa 1442



46
258
PRT
Oryza sativa

46

Met Glu Ser Arg Pro Gly Gly Thr Asn Leu Val Glu Pro Arg Gly Gln
1 5 10 15

Gly Ala Leu Pro Ser Gly Ile Pro Ile Gln Gln Pro Trp Trp Thr Thr
20 25 30

Ser Ala Gly Val Gly Ala Val Ser Pro Ala Val Val Ala Pro Gly Ser
35 40 45

Gly Ala Gly Ile Ser Leu Ser Gly Arg Asp Gly Gly Gly Asp Asp Ala
50 55 60

Ala Glu Glu Ser Ser Asp Asp Ser Arg Arg Ser Gly Glu Thr Lys Asp
65 70 75 80

Gly Ser Thr Asp Gln Glu Lys His His Ala Thr Ser Gln Met Thr Ala
85 90 95

Leu Ala Ser Asp Tyr Leu Thr Pro Phe Ser Gln Leu Glu Leu Asn Gln
100 105 110

Pro Ile Ala Ser Ala Ala Tyr Gln Tyr Pro Asp Ser Tyr Tyr Met Gly
115 120 125

Met Val Gly Pro Tyr Gly Pro Gln Ala Met Ser Ala Gln Thr His Phe
130 135 140

Gln Leu Pro Gly Leu Thr His Ser Arg Met Pro Leu Pro Leu Glu Ile
145 150 155 160

Ser Glu Glu Pro Val Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu
165 170 175

Arg Arg Arg Gln Ser Arg Ala Lys Ala Glu Leu Glu Lys Lys Val Val
180 185 190

Lys Ser Arg Lys Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met
195 200 205

Arg Arg Ala Arg Gly Thr Gly Gly Arg Phe Leu Asn Thr Lys Lys Asn
210 215 220

Glu Asp Gly Ala Pro Ser Glu Lys Ala Glu Pro Asn Lys Gly Glu Gln
225 230 235 240

Asn Ser Gly Tyr Arg Arg Ile Pro Pro Asp Leu Gln Leu Leu Gln Lys
245 250 255

Glu Thr



47
423
DNA
Oryza sativa

unsure
(223)
n = A, C, G, or T


47

cattggttct aatcttgaca gagtttaagg ttggcacttt ctgtcagaag ttaagttagg 60

acttccacaa aattatacca tctctgggtg ttcttatagg tgtttctcac tatcaggaat 120

gtacagttct tgcagctgtc aagttctttg tacctatgtt tctgtatctt ctaaagattt 180

tgattcgtct gcactgtgca gccatatctc catgagtcac ggnatcaaca tgccctgaaa 240

agggctaggg gagctggagg ccgatttctt aattcaaaat cggatgacaa ggaaagagca 300

ttctgattcc aagttccaag agataaacan gatggagttg cacccccgtg ataatgggca 360

aacgtctanc tctccgtctt caaaggggng gatcatcagc tnaacaaaat aaagaagtca 420

aaa 423



48
34
PRT
Oryza sativa

UNSURE
(9)
Xaa = any amino acid


48

Gln Pro Tyr Leu His Glu Ser Arg Xaa Gln His Ala Leu Lys Arg Ala
1 5 10 15

Arg Gly Ala Gly Gly Arg Phe Leu Asn Ser Lys Ser Asp Asp Lys Glu
20 25 30

Arg Ala



49
479
DNA
Oryza sativa

49

ctcttctcat ctcatctccc tctcctctcc tctcgccgtc gccgtcgccg tcgccgccgc 60

tcgccgccgg cggggataga gttcgccggg atcgcctcgc cgggagagtt ccctcaccat 120

cccgcacctc cgctcgcctg gcctcttcct cccggaagtg tggtgtgctg caagctcctg 180

tctctcctac aaggtttcaa aaccaaaata tgcctgaagc acacggaaag ctggggtgat 240

taacgtctgt ttcttttgac tacaatcatc ctgattctgc ttctgtctgc aaaaacaacc 300

aagccatgac gtctgtagtt catgatgttt caggcaacca tggagctgat gagcggcaaa 360

aacagcaaag gcaaggtgaa cctgaggacc aagcaagaag cctcagttac tagtacagat 420

agccatacaa tggtaagcaa caccttcaac agattatgcg acaacctatg cccatcacg 479



50
35
PRT
Oryza sativa

50

Met Thr Ser Val Val His Asp Val Ser Gly Asn His Gly Ala Asp Glu
1 5 10 15

Arg Gln Lys Gln Gln Arg Gln Gly Glu Pro Glu Asp Gln Ala Arg Ser
20 25 30

Leu Ser Tyr
35



51
1107
DNA
Oryza sativa

51

gcacgagcaa ttatccttgt attgaccaat gctatggtct tatgaccacc tacgcgatga 60

aatcaatgag tggcgggcga atgctactgc cgctgaacgc gccagccgat gcgccgatct 120

atgtcaacgc gaagcagtac gaaggcatcc tccgccgtcg ccgtgcccgc gccaaggccc 180

agagggagaa caggctggtc aaaggcagga agccctacct ccacgagtcg cgccaccgcc 240

acgccatgcg ccgggccaga ggctccggcg gccgcttcct caacaccaag aaagaagcca 300

ccgccgccgg atgcggcggc agcagcaaga cgcccctcgc gtccctcgtc agccccgccg 360

acgtagccca tcgtccaggc tccggcggcc gcgcgtccag cctctccggc tccgacgtgt 420

cgtcgccggg aggcgtcatg tacgaccacc accgccacga cgacgccgac gcggcggacc 480

actacaacag catcgaccac cacctccgca cgccgttctt caccccgctc ccgatcatca 540

tggacagcgg cggcggcggc ggcgaccacg cctcacactc cgccgccgcc gtcgccgccc 600

ccttcaggtg ggcgacggcg gccggcgacg gctgctgcga gctcctcaag gcgtgacagc 660

cttgaggcgg ggatctccag gcgtgcccag agctgctgct gatcgatcac catcagcttt 720

ggctgcctgt aggcaaatca ttcttggctc tttacttgca ttggggttct tgcaagcaac 780

tctcctcgtc acctaccaaa actgtccctg aaacttctct agtgctgggg tctcgatcag 840

ggatgatgat gtgatggagg agaggcttac ccatatgcct gtaaattatg gttagtgttc 900

tgattaagca actagtagta cttggtaatt actggctatg aattagtagt atggactctg 960

gtgtcaggtt gctctttgtc tgaataaact ggagtcgttt gaagctttgc aaaaaaaaaa 1020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1080

aaaaaaaaaa aaaaaaaaaa aaaaaaa 1107



52
217
PRT
Oryza sativa

52

Thr Ser Asn Tyr Pro Cys Ile Asp Gln Cys Tyr Gly Leu Met Thr Thr
1 5 10 15

Tyr Ala Met Lys Ser Met Ser Gly Gly Arg Met Leu Leu Pro Leu Asn
20 25 30

Ala Pro Ala Asp Ala Pro Ile Tyr Val Asn Ala Lys Gln Tyr Glu Gly
35 40 45

Ile Leu Arg Arg Arg Arg Ala Arg Ala Lys Ala Gln Arg Glu Asn Arg
50 55 60

Leu Val Lys Gly Arg Lys Pro Tyr Leu His Glu Ser Arg His Arg His
65 70 75 80

Ala Met Arg Arg Ala Arg Gly Ser Gly Gly Arg Phe Leu Asn Thr Lys
85 90 95

Lys Glu Ala Thr Ala Ala Gly Cys Gly Gly Ser Ser Lys Thr Pro Leu
100 105 110

Ala Ser Leu Val Ser Pro Ala Asp Val Ala His Arg Pro Gly Ser Gly
115 120 125

Gly Arg Ala Ser Ser Leu Ser Gly Ser Asp Val Ser Ser Pro Gly Gly
130 135 140

Val Met Tyr Asp His His Arg His Asp Asp Ala Asp Ala Ala Asp His
145 150 155 160

Tyr Asn Ser Ile Asp His His Leu Arg Thr Pro Phe Phe Thr Pro Leu
165 170 175

Pro Ile Ile Met Asp Ser Gly Gly Gly Gly Gly Asp His Ala Ser His
180 185 190

Ser Ala Ala Ala Val Ala Ala Pro Phe Arg Trp Ala Thr Ala Ala Gly
195 200 205

Asp Gly Cys Cys Glu Leu Leu Lys Ala
210 215



53
977
DNA
Oryza sativa

53

gcacgaggca aactctagga tgccattgcc tgttgatcct tctgtagaag agcccatatt 60

tgtcaatgca aagcaataca atgcgatcct tagaagaagg caaacgcgtg caaaattgga 120

ggcccaaaat aaggcggtga aaggtcggaa gccttacctc catgaatctc gacatcatca 180

tgctatgaag cgagcccgtg gatcaggtgg tcggttcctt accaaaaagg agctgctgga 240

acagcagcag cagcagcagc agcagaagcc accaccggca tcagctcagt ctccaacagg 300

tagagccaga acgagcggcg gtgccgttgt ccttggcaag aacctgtgcc cagagaacag 360

cacatcctgc tcgccatcga caccgacagg ctccgagatc tccagcatct catttggggg 420

cggcatgctg gctcaccaag agcacatcag cttcgcatcc gctgatcgcc accccacaat 480

gaaccagaac caccgtgtcc ccgtcatgag gtgaaaacct cgggatcgcg ggacacgggc 540

ggttctggtt taccctcact ggcgcactcc ggtgtgcccg tggcaattca tccttggctt 600

atgaagtatc tacctgataa tagtctgctg tcagtttata tgcaatgcaa cctctgtcag 660

ataaactctt atagtttgtt ttattgtaag ctatgactga acgaactgtc gagcagatgg 720

ctaatttgta tgttgtgggt acagaaatcc tgaagctttt gatgtaccta attgcctttt 780

gcttatactc ttggtgtata cccattacca agttgcctta aaaaccctcc aattatgtaa 840

tcagtcatgg ttttatagaa ccttgccaca tgtaatcaat cacctgtttt tgtaaattga 900

tctataaacg ctataggctg ctgtgttatc tgcatttaaa aaaaaaaaaa aaaaaaaaaa 960

aaaaaaaaaa aaaaaaa 977



54
168
PRT
Oryza sativa

54

Ala Asn Ser Arg Met Pro Leu Pro Val Asp Pro Ser Val Glu Glu Pro
1 5 10 15

Ile Phe Val Asn Ala Lys Gln Tyr Asn Ala Ile Leu Arg Arg Arg Gln
20 25 30

Thr Arg Ala Lys Leu Glu Ala Gln Asn Lys Ala Val Lys Gly Arg Lys
35 40 45

Pro Tyr Leu His Glu Ser Arg His His His Ala Met Lys Arg Ala Arg
50 55 60

Gly Ser Gly Gly Arg Phe Leu Thr Lys Lys Glu Leu Leu Glu Gln Gln
65 70 75 80

Gln Gln Gln Gln Gln Gln Lys Pro Pro Pro Ala Ser Ala Gln Ser Pro
85 90 95

Thr Gly Arg Ala Arg Thr Ser Gly Gly Ala Val Val Leu Gly Lys Asn
100 105 110

Leu Cys Pro Glu Asn Ser Thr Ser Cys Ser Pro Ser Thr Pro Thr Gly
115 120 125

Ser Glu Ile Ser Ser Ile Ser Phe Gly Gly Gly Met Leu Ala His Gln
130 135 140

Glu His Ile Ser Phe Ala Ser Ala Asp Arg His Pro Thr Met Asn Gln
145 150 155 160

Asn His Arg Val Pro Val Met Arg
165



55
465
DNA
Oryza sativa

unsure
(280)
n = A, C, G, or T


55

cttacagcag ccttaccttc acgaatctcg gcatcgccat gcaatgaaga gggctagggg 60

cactggtggg cgattcctga ataccaagca gctccagctg cagcaacagt ctcacactac 120

ctccaccaag accaccacag acagccaaaa ttcttcaggt tcaagtcatc tacggctagg 180

tggtggcgca atcggagatc aaactccatt tccgttcaaa gcaatggatt cacaagctaa 240

catcaagaga gctgcagctt ctgcttccac cttcactgtn acttctgcgg gacaaaaaga 300

cgacgccttc ttcgaccgcc atggncaaca tctcaataac ttctccggnc attttggnca 360

agcaagcnca caaaggggng tcggnaagca tgcataaccg gtcaaaagca agagggttcc 420

tgctnatgnn gatganatga aagagcagct tggaaatcna acant 465



56
131
PRT
Oryza sativa

UNSURE
(123)
Xaa = any amino acid


56

Leu Gln Gln Pro Tyr Leu His Glu Ser Arg His Arg His Ala Met Lys
1 5 10 15

Arg Ala Arg Gly Thr Gly Gly Arg Phe Leu Asn Thr Lys Gln Leu Gln
20 25 30

Leu Gln Gln Gln Ser His Thr Thr Ser Thr Lys Thr Thr Thr Asp Ser
35 40 45

Gln Asn Ser Ser Gly Ser Ser His Leu Arg Leu Gly Gly Gly Ala Ile
50 55 60

Gly Asp Gln Thr Pro Phe Pro Phe Lys Ala Met Asp Ser Gln Ala Asn
65 70 75 80

Ile Lys Arg Ala Ala Ala Ser Ala Ser Thr Phe Thr Val Thr Ser Ala
85 90 95

Gly Gln Lys Asp Asp Ala Phe Phe Asp Arg His Gly Gln His Leu Asn
100 105 110

Asn Phe Ser Gly His Phe Gly Gln Ala Ser Xaa Gln Arg Gly Val Gly
115 120 125

Lys His Ala
130



57
1482
DNA
Glycine max

57

tttctgttct tctctgggga tctgaagaca tgcagtccaa gtctgaaact gcaaatcgac 60

tgagatcaga tcctcattcc tttcaacctg gcagtgttta ttctgagcct tggtggcgtg 120

gtattgggta caatcctgtg gcccaaacaa tggctggggc aaatgcatcc aattcatcgt 180

ctcttgaatg ccctaatggt gattctgaat ccaatgaaga aggtcaatct ttgtccaata 240

gcgggatgaa tgaggaagat gatgatgcca ctaaggattc acagcctgct gttcctaatg 300

gaacaggaaa ttatgggcaa gaacagcaag ggatgcagca tactgcatca tctgcaccct 360

ccatgcgtga agaatgcctt actcagacac cacagctgga acttgtcggt cattcaattg 420

catgtgctac aaatccttat caggatccgt attatggggg catgatggca gcttatggtc 480

accaacagtt gggatatgct ccttttatag gaatgcctca tgccagaatg cctttgcccc 540

ttgagatggc tcaagaacct gtgtatgtga atgccaaaca gtaccaagga attctgaggc 600

gaagacaggc tcgtgctaaa gcagagcttg aaaggaagct cataaaatct agaaagccat 660

atcttcatga atctaggcat cagcatgcta tgagaagggc aaggggtact ggaggacgat 720

ttgcaaagaa aactgacggt gagggctcaa accactcagg caaggaaaag gataatggta 780

ctgattctgt cctatcatca caatcaatta gttcatctgg ttctgaacct ttacattctg 840

actctgccga aacctggaat tctcctaaca tgcaacaaga tgcaagagca tcaaaagtgc 900

acaacaggtt caaagcaccc tgttaccaaa atggcagtgg ctcctaccat aatcataatg 960

gattgcaatc ttcagtgtac cattcatcct caggtgaaag actggaggaa agggattgtt 1020

cgggtcagca actgaaccac aattgatggg gggttagagg ccgaggttgg tttgtatcca 1080

agtgacatat ttggtgaata ccttggttat ctgtaaacac tcttggcaat atatatgcca 1140

agcggcaaat cattcttggc tttgttcttg tgtttgtggt gttaatgata ctatgggggg 1200

ggtggggggg gggggaatga ttggtatttg agatttctgt tgaagtcagt caatcaatcc 1260

ttcgttcttt tctcattttt gcattttgta aagttttata gtggttagga tggtcacttc 1320

agaagattat ggagtatggt gagaaacaaa ctcttgatgt gccaacactc gtttgactgg 1380

tttatctttg tgtagttcaa ccggttgtta atgttaacat aagacatcat aggataatga 1440

acatgctgtt agttacatta catcaaaaaa aaaaaaaaaa aa 1482



58
338
PRT
Glycine max

58

Met Gln Ser Lys Ser Glu Thr Ala Asn Arg Leu Arg Ser Asp Pro His
1 5 10 15

Ser Phe Gln Pro Gly Ser Val Tyr Ser Glu Pro Trp Trp Arg Gly Ile
20 25 30

Gly Tyr Asn Pro Val Ala Gln Thr Met Ala Gly Ala Asn Ala Ser Asn
35 40 45

Ser Ser Ser Leu Glu Cys Pro Asn Gly Asp Ser Glu Ser Asn Glu Glu
50 55 60

Gly Gln Ser Leu Ser Asn Ser Gly Met Asn Glu Glu Asp Asp Asp Ala
65 70 75 80

Thr Lys Asp Ser Gln Pro Ala Val Pro Asn Gly Thr Gly Asn Tyr Gly
85 90 95

Gln Glu Gln Gln Gly Met Gln His Thr Ala Ser Ser Ala Pro Ser Met
100 105 110

Arg Glu Glu Cys Leu Thr Gln Thr Pro Gln Leu Glu Leu Val Gly His
115 120 125

Ser Ile Ala Cys Ala Thr Asn Pro Tyr Gln Asp Pro Tyr Tyr Gly Gly
130 135 140

Met Met Ala Ala Tyr Gly His Gln Gln Leu Gly Tyr Ala Pro Phe Ile
145 150 155 160

Gly Met Pro His Ala Arg Met Pro Leu Pro Leu Glu Met Ala Gln Glu
165 170 175

Pro Val Tyr Val Asn Ala Lys Gln Tyr Gln Gly Ile Leu Arg Arg Arg
180 185 190

Gln Ala Arg Ala Lys Ala Glu Leu Glu Arg Lys Leu Ile Lys Ser Arg
195 200 205

Lys Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg Ala
210 215 220

Arg Gly Thr Gly Gly Arg Phe Ala Lys Lys Thr Asp Gly Glu Gly Ser
225 230 235 240

Asn His Ser Gly Lys Glu Lys Asp Asn Gly Thr Asp Ser Val Leu Ser
245 250 255

Ser Gln Ser Ile Ser Ser Ser Gly Ser Glu Pro Leu His Ser Asp Ser
260 265 270

Ala Glu Thr Trp Asn Ser Pro Asn Met Gln Gln Asp Ala Arg Ala Ser
275 280 285

Lys Val His Asn Arg Phe Lys Ala Pro Cys Tyr Gln Asn Gly Ser Gly
290 295 300

Ser Tyr His Asn His Asn Gly Leu Gln Ser Ser Val Tyr His Ser Ser
305 310 315 320

Ser Gly Glu Arg Leu Glu Glu Arg Asp Cys Ser Gly Gln Gln Leu Asn
325 330 335

His Asn



59
1385
DNA
Glycine max

59

gcacgagggg attttgagtg gaggggaaaa gttgtgctaa gatgccgggg aaagctgaca 60

ctgatgattg gcgagtagag cggggtgagc agattcagtt tcagtcttcc atttactctc 120

atcatcagcc ttggtggtgt ggagtggggg aaaatgcctc taaatcatct tcagctgatc 180

agttaaatgg ttcaatcgtg aatggtatca cgcggtctga gaccaatgat aagtcaggtg 240

aaggtgttgc caaagaatac caaaacatca aacatgccgt gttgtcaacc ccatttacca 300

tggacaaaca tcttgctcca aatccccaga tggaacttgt tggtcattca gttgttttaa 360

catctcctta ttcagatgca cagcatggtc aaatcttgac tacttacggg caacaagtta 420

tgataaaccc tcaattgtac ggaatgtatc atgctagaat gcctttgcca cctgaaatgg 480

aagaggagcc tgtttatgtc aatgcaaagc agtatcatgg tattttgagg cgaagacagt 540

cacgtgctaa ggctgagctt gaaaagaaag taatcaaaaa caggaagcca tacctccatg 600

aatcccgtca ccttcatgcc atgagaaggg ctagaggcaa tggtggtcgc tttctcaaca 660

aaaagaagct cgaaaattac aattctgatg ccacttcaga cattgggcaa aatactggtg 720

caaacccctc aacaaactca cctaacactc aacatttgtt caccaacaat gagaatctag 780

gctcatcaaa tgcgtcacaa gccacggttc aggacatgca cagagtggag agtttcaata 840

ttggttacca taatggaaat ggtcttgcag aactgtacca ttcacaagca aatggaaaaa 900

aggagggaaa ctgctttggt aaagagaggg accctaataa tggggctttc aaatgacact 960

tcgcccagcc atacagcaac agttaggtga agatgaaggg tttttatctc atccaacttg 1020

tgatgctgta ttgaaggcaa ttcattcttg gcttagttaa gtggtgagac cagtgacatg 1080

gagtacactc tgccttgttt ggtctctccc cttgcatttg tttctcttta caagtccata 1140

tgtaaaaatg gataacggaa agaaaaagaa aaatcacttt tgtttgagaa cttttttaag 1200

tttgttttta actgtgtgaa ggtttcataa aattgtggac tgacttgtgt gacatatgct 1260

ccacaaaacc ttaaaacttt cgtctatttt gtccaaaaaa aaaaaaaaaa aaaaaaaaaa 1320

aaaaaaaaaa aaagggaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1380

aaaaa 1385



60
304
PRT
Glycine max

60

Met Pro Gly Lys Ala Asp Thr Asp Asp Trp Arg Val Glu Arg Gly Glu
1 5 10 15

Gln Ile Gln Phe Gln Ser Ser Ile Tyr Ser His His Gln Pro Trp Trp
20 25 30

Cys Gly Val Gly Glu Asn Ala Ser Lys Ser Ser Ser Ala Asp Gln Leu
35 40 45

Asn Gly Ser Ile Val Asn Gly Ile Thr Arg Ser Glu Thr Asn Asp Lys
50 55 60

Ser Gly Glu Gly Val Ala Lys Glu Tyr Gln Asn Ile Lys His Ala Val
65 70 75 80

Leu Ser Thr Pro Phe Thr Met Asp Lys His Leu Ala Pro Asn Pro Gln
85 90 95

Met Glu Leu Val Gly His Ser Val Val Leu Thr Ser Pro Tyr Ser Asp
100 105 110

Ala Gln His Gly Gln Ile Leu Thr Thr Tyr Gly Gln Gln Val Met Ile
115 120 125

Asn Pro Gln Leu Tyr Gly Met Tyr His Ala Arg Met Pro Leu Pro Pro
130 135 140

Glu Met Glu Glu Glu Pro Val Tyr Val Asn Ala Lys Gln Tyr His Gly
145 150 155 160

Ile Leu Arg Arg Arg Gln Ser Arg Ala Lys Ala Glu Leu Glu Lys Lys
165 170 175

Val Ile Lys Asn Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu His
180 185 190

Ala Met Arg Arg Ala Arg Gly Asn Gly Gly Arg Phe Leu Asn Lys Lys
195 200 205

Lys Leu Glu Asn Tyr Asn Ser Asp Ala Thr Ser Asp Ile Gly Gln Asn
210 215 220

Thr Gly Ala Asn Pro Ser Thr Asn Ser Pro Asn Thr Gln His Leu Phe
225 230 235 240

Thr Asn Asn Glu Asn Leu Gly Ser Ser Asn Ala Ser Gln Ala Thr Val
245 250 255

Gln Asp Met His Arg Val Glu Ser Phe Asn Ile Gly Tyr His Asn Gly
260 265 270

Asn Gly Leu Ala Glu Leu Tyr His Ser Gln Ala Asn Gly Lys Lys Glu
275 280 285

Gly Asn Cys Phe Gly Lys Glu Arg Asp Pro Asn Asn Gly Ala Phe Lys
290 295 300



61
1401
DNA
Glycine max

61

gaagtcttta tgtgacctgg gtggaatgat tctgtgtctg catgtgtgaa ttctggcaag 60

ggaactaggg atctgaagat aagatatgca atctaaatct gaaactgcaa atcaactgag 120

gtctgatcca cattccttta cacctaacaa tgcttattct gaaccctggt ggcgaggtat 180

tcagtacaat cctgtccccc aagcaatgtt aggagtgaat gcatctaatt catcttcact 240

tgaacgccct aatggtgatt cggaatccag tgaagaggat gatgatgcca ctaaagaatc 300

acaacccact gctcctaatc aatcaggaaa ttatggacag gaccaccaag cgatgcaaca 360

ttcttcatca tctgcacctt tggtacgtga tgattgcctt acacaggctc cacaagtgga 420

acttgttggc cactcaattg gatacactcc ttttatagga atgccccatg ccagaatggc 480

tttgcccctt gagatggctc aagagcctgt ttatgtgaat gccaaacaat accaaggaat 540

tctgagacga agacaggctc gtgctaaagc agagcttgaa aagaaattaa taaaagtcag 600

aaagccatat cttcatgaat cccggcatca gcatgctata agaagagcac gaggtaatgg 660

agggcgtttt gcaaagaaaa ctgaagttga ggcttcaaac cacatgaaca aggaaaagga 720

tatgggtact ggccaggtcc cattgtcacg gtcaattagt tcatctggtt ttggatcact 780

accctctgac tctgctgaga cctggaattc tcctagtgtg caacaagatg caagaggatc 840

tcaagtgcat gagagatttg aagaacgcaa ctatgcaaat gttttgcagt catcatctac 900

tttttgtttg cactcgggtg aaagagtgga ggaaggggac tgttcaggtc aacaacgggg 960

aagcatcttg tcagagcaca cctcacagag gcgtcttgct attcagtaaa ccactgcatg 1020

tgttgatgct gaggttggta tatataattg agtgaactag taggttgagt accttggcta 1080

tctatctgta aacattggca atttgcatgc atgtcaagcg gcaaatcatt cttggctggg 1140

tttcagctgt tcatgatatg gggagaagaa tgattgattg ggccatcata cttgtgttgt 1200

tgaagtctac cagtccttca ttatatcctc tttttcattt tttctgtttt tgtacagaga 1260

tagtagttag caaagtcaag ccaacggatt agaagacttg atgaaacaaa ctactgactc 1320

actttcctct ggcggcttta ttttatgtta ctcaccggtt attaatgctt aatatgagac 1380

atcatatgag agatttgctg c 1401



62
307
PRT
Glycine max

62

Met Gln Ser Lys Ser Glu Thr Ala Asn Gln Leu Arg Ser Asp Pro His
1 5 10 15

Ser Phe Thr Pro Asn Asn Ala Tyr Ser Glu Pro Trp Trp Arg Gly Ile
20 25 30

Gln Tyr Asn Pro Val Pro Gln Ala Met Leu Gly Val Asn Ala Ser Asn
35 40 45

Ser Ser Ser Leu Glu Arg Pro Asn Gly Asp Ser Glu Ser Ser Glu Glu
50 55 60

Asp Asp Asp Ala Thr Lys Glu Ser Gln Pro Thr Ala Pro Asn Gln Ser
65 70 75 80

Gly Asn Tyr Gly Gln Asp His Gln Ala Met Gln His Ser Ser Ser Ser
85 90 95

Ala Pro Leu Val Arg Asp Asp Cys Leu Thr Gln Ala Pro Gln Val Glu
100 105 110

Leu Val Gly His Ser Ile Gly Tyr Thr Pro Phe Ile Gly Met Pro His
115 120 125

Ala Arg Met Ala Leu Pro Leu Glu Met Ala Gln Glu Pro Val Tyr Val
130 135 140

Asn Ala Lys Gln Tyr Gln Gly Ile Leu Arg Arg Arg Gln Ala Arg Ala
145 150 155 160

Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys Val Arg Lys Pro Tyr Leu
165 170 175

His Glu Ser Arg His Gln His Ala Ile Arg Arg Ala Arg Gly Asn Gly
180 185 190

Gly Arg Phe Ala Lys Lys Thr Glu Val Glu Ala Ser Asn His Met Asn
195 200 205

Lys Glu Lys Asp Met Gly Thr Gly Gln Val Pro Leu Ser Arg Ser Ile
210 215 220

Ser Ser Ser Gly Phe Gly Ser Leu Pro Ser Asp Ser Ala Glu Thr Trp
225 230 235 240

Asn Ser Pro Ser Val Gln Gln Asp Ala Arg Gly Ser Gln Val His Glu
245 250 255

Arg Phe Glu Glu Arg Asn Tyr Ala Asn Val Leu Gln Ser Ser Ser Thr
260 265 270

Phe Cys Leu His Ser Gly Glu Arg Val Glu Glu Gly Asp Cys Ser Gly
275 280 285

Gln Gln Arg Gly Ser Ile Leu Ser Glu His Thr Ser Gln Arg Arg Leu
290 295 300

Ala Ile Gln
305



63
1241
DNA
Glycine max

63

gcacgaggtc ctaagttgta agaaacactc tcttctcctt tctcactatt gttctgttac 60

tgttttttgc agcaacactt cagttcaatt aacgaactac accactttct ttctcttctt 120

cgactgctct gtaaccgaaa acctcccttt cccagtttcg aatcttttgt ttctgccttt 180

ggttactgtt tttccgagcc atgctattca ttattgtcct tcgaatcgga ttgattggga 240

cactgtattg catgtaaatc aggaaatcat gacttctact catgacctct cagataatga 300

agctgatgac cagcagcagt cggaatcaca aatggagcct ttatctgcaa atggaatttc 360

ttatgcaggt attgctactc agaatgttca gtatgcaaca ccttcacagc ttggaactgg 420

gcatgctgtg gtaccgccca cttacccata tccagatcca tactacagaa gtatctttgc 480

tccctatgat gcacaaactt atcccccaca accctatggt ggaaatccaa tggtccacct 540

tcagttaatg ggaattcaac aagcaggtgt tcctttgcca actgatacag ttgaggagcc 600

tgtgtttgtc aatgcaaaac agtatcatgg tatattaaga cgcagacagt cccgtgctaa 660

agctgaatca gaaaaaaagg ctgcaaggaa tcggaagcca tacttgcatg aatctcgaca 720

tttgcatgca ctgagaagag caagaggatg tggaggtcgg tttttgaatt caaagaaaga 780

tgagaatcaa caggatgagg ttgcatcaac tgacgaatca cagtccacta tcaatctcaa 840

ttctgataaa aatgagcttg caccatcaga tagaacatcc taaaactaca gaaatggtga 900

tgctgtagat tgcagggatc tgttgtgtat atctatattg ggagatgaat ctccaaccaa 960

cagtatcctc agatatctcc ctattattca ttctgtcgta caacgccata ggtataagta 1020

taggttgtgt agtaggtatg ttaggaggtt gcaaaataaa acaagtaaaa tgtaaattga 1080

agtgattcaa ctaagtctat ccccaatgtg gtcctttctt gcctttttag gtatttttat 1140

tgtgtgggct tttctttgta ttatttggtg cctctgaggg aaagagaaga gattatccga 1200

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 1241



64
204
PRT
Glycine max

64

Met Thr Ser Thr His Asp Leu Ser Asp Asn Glu Ala Asp Asp Gln Gln
1 5 10 15

Gln Ser Glu Ser Gln Met Glu Pro Leu Ser Ala Asn Gly Ile Ser Tyr
20 25 30

Ala Gly Ile Ala Thr Gln Asn Val Gln Tyr Ala Thr Pro Ser Gln Leu
35 40 45

Gly Thr Gly His Ala Val Val Pro Pro Thr Tyr Pro Tyr Pro Asp Pro
50 55 60

Tyr Tyr Arg Ser Ile Phe Ala Pro Tyr Asp Ala Gln Thr Tyr Pro Pro
65 70 75 80

Gln Pro Tyr Gly Gly Asn Pro Met Val His Leu Gln Leu Met Gly Ile
85 90 95

Gln Gln Ala Gly Val Pro Leu Pro Thr Asp Thr Val Glu Glu Pro Val
100 105 110

Phe Val Asn Ala Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln Ser
115 120 125

Arg Ala Lys Ala Glu Ser Glu Lys Lys Ala Ala Arg Asn Arg Lys Pro
130 135 140

Tyr Leu His Glu Ser Arg His Leu His Ala Leu Arg Arg Ala Arg Gly
145 150 155 160

Cys Gly Gly Arg Phe Leu Asn Ser Lys Lys Asp Glu Asn Gln Gln Asp
165 170 175

Glu Val Ala Ser Thr Asp Glu Ser Gln Ser Thr Ile Asn Leu Asn Ser
180 185 190

Asp Lys Asn Glu Leu Ala Pro Ser Asp Arg Thr Ser
195 200



65
1716
DNA
Glycine max

65

gcacgaggta cgtaccgaca tgactccaac ctgatggggt taaacactgc ttctgcgtag 60

gattcgatgc cgctactcct tcttcagttt ctacaactga gtttcatatc tcctttctat 120

tgatgtttat gctgaagact gaataaaagt ctgagaaagc tgcttactac aaaccaacaa 180

gattaactaa gaaatcatct tttgggacga tgcaaactgt ttatcttaaa gagcacgaag 240

gaaatgcgca caattttgtg ggcacgttgt cttctgcagc ttcagcaccc tggtggagtg 300

cttttggatc tcaatctgtt catcagggag agtcttgtgg ccaagtgaaa cccttttcat 360

tggagctgcc aaactgcata gaccaacttg ctgccactaa gccactagca agaggagctg 420

accaagtgtt gggtaaaggg cacataactc agtttacaat ctttccagat gattgtaaaa 480

tgtcagatga tgcgcaaaag cttcagacaa ccatgtcact gcagtcatcg cttactgatc 540

cacagtctcg ttttgagata gggtttagtc tgcccacgat atgtgcaaaa tatccttata 600

cggatcaatt ttatggactc ttctcagctt atgcacctca aatttcggga cgtataatgc 660

tgccacttaa catgacatct gatgatgaac caatttacgt aaatgctaag cagtaccatg 720

gaatcattag acgtcggcag tcccgtgcca aagctgtact tgatcacaaa ttgactaaac 780

gtcgcaagcc ctatatgcac gaatcacgcc atctccatgc aatgcggcga ccaagaggat 840

gtgggggtcg cttcttgaac actaagaatt ctgttgacgg aaatggtaaa attggaaatg 900

aagtgcataa aactgttggt gaacaattgc agtctagtgg ctctcagagt tctgaattcc 960

ttcaatctga ggttggaact tttaattcat caaaagagac taatggaagc agtccaaata 1020

tttctggttc agaggtgact agcatgtatt cgcggggagg tcttgacagc ttttctctca 1080

atcatcttgg atctgctgtc cactcttttg cagacatgat agatggtggg cgcggtatga 1140

tcatacccac caaatgggtt gcagcagcag gtaactgctg caaccttaaa gtttgatttg 1200

caaagaatca agggtgggct tgctgtagca ttgcaccagg cccatcctcg atgaggccag 1260

atgaagaagc ttcgtttcag ttgcgtgtgc tgactgtgac aagtttcgct cggtaagatc 1320

gtcctcacat ctggtctagg caatccatcc ttggctcata ctttggcaat ccatccttgg 1380

ctcattgtaa ctgaaggcaa ctcatccttg gcttgatgta cttgcagtaa tttgtctttc 1440

tgcacaggaa tgttgttggc atggtacaaa ctaatgactt gatatcctga tgcagaagac 1500

aactatgttt ctgtctttgt gtgaaaatga aagcatgaaa ctctagttat gtgtgcttcg 1560

aataatgtct aaacgtggtg ttgtattttg tatttctgac ttcgaggaac aatgtattat 1620

agaaccttgt tctgtggtct ttgttagaaa aaataaagca ttggtgtgtt tttctccaaa 1680

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaag 1716



66
328
PRT
Glycine max

66

Met Gln Thr Val Tyr Leu Lys Glu His Glu Gly Asn Ala His Asn Phe
1 5 10 15

Val Gly Thr Leu Ser Ser Ala Ala Ser Ala Pro Trp Trp Ser Ala Phe
20 25 30

Gly Ser Gln Ser Val His Gln Gly Glu Ser Cys Gly Gln Val Lys Pro
35 40 45

Phe Ser Leu Glu Leu Pro Asn Cys Ile Asp Gln Leu Ala Ala Thr Lys
50 55 60

Pro Leu Ala Arg Gly Ala Asp Gln Val Leu Gly Lys Gly His Ile Thr
65 70 75 80

Gln Phe Thr Ile Phe Pro Asp Asp Cys Lys Met Ser Asp Asp Ala Gln
85 90 95

Lys Leu Gln Thr Thr Met Ser Leu Gln Ser Ser Leu Thr Asp Pro Gln
100 105 110

Ser Arg Phe Glu Ile Gly Phe Ser Leu Pro Thr Ile Cys Ala Lys Tyr
115 120 125

Pro Tyr Thr Asp Gln Phe Tyr Gly Leu Phe Ser Ala Tyr Ala Pro Gln
130 135 140

Ile Ser Gly Arg Ile Met Leu Pro Leu Asn Met Thr Ser Asp Asp Glu
145 150 155 160

Pro Ile Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Ile Arg Arg Arg
165 170 175

Gln Ser Arg Ala Lys Ala Val Leu Asp His Lys Leu Thr Lys Arg Arg
180 185 190

Lys Pro Tyr Met His Glu Ser Arg His Leu His Ala Met Arg Arg Pro
195 200 205

Arg Gly Cys Gly Gly Arg Phe Leu Asn Thr Lys Asn Ser Val Asp Gly
210 215 220

Asn Gly Lys Ile Gly Asn Glu Val His Lys Thr Val Gly Glu Gln Leu
225 230 235 240

Gln Ser Ser Gly Ser Gln Ser Ser Glu Phe Leu Gln Ser Glu Val Gly
245 250 255

Thr Phe Asn Ser Ser Lys Glu Thr Asn Gly Ser Ser Pro Asn Ile Ser
260 265 270

Gly Ser Glu Val Thr Ser Met Tyr Ser Arg Gly Gly Leu Asp Ser Phe
275 280 285

Ser Leu Asn His Leu Gly Ser Ala Val His Ser Phe Ala Asp Met Ile
290 295 300

Asp Gly Gly Arg Gly Met Ile Ile Pro Thr Lys Trp Val Ala Ala Ala
305 310 315 320

Gly Asn Cys Cys Asn Leu Lys Val
325



67
1103
DNA
Glycine max

67

gcacgaggaa atgaagaatt agagggagtg agaggaggaa gaagaagaag aagattccag 60

aatccagagt gagaaacatt aggcttatca gaggagacat gcccgagttg aaccgacaat 120

tctattacta ctctttgctt ctttcttcat gcctcatcaa atcccaaagg atataattga 180

aggttttggg aactaaggct gcaatattgt atacattcta ctcaaggaat ggctcatact 240

tcttatcctt gtggtgatcc ttattttggt agttcaatag ttgcttatgg aacacaggct 300

attactcaac aaatggtgcc ccagatgctg ggattagcat ccaccagaat tgcattacca 360

gttgagcttg cagaagatgg gcccatttat gtcaatgcca aacaatacca tggtatactg 420

agaaggcgac agtcacgagc aaagcttaag gctcaaaaca aactcatcaa aagtcgtaag 480

ccatatcttc atgagtctcg gcaccgccac gcattgaaaa gggttagggg aactgggggg 540

cgctttctta gtgccaaaca gcttcaacag tttaatgcag aacttgtcac cgatgcccat 600

tcaggcccgg gccctgtcaa tgtttatcaa aagaaagatg catctgaggc agaaagtcat 660

ccctcaagaa ctggaaaaaa tgcatctatc acattcacag caatctctgg cttgacaagt 720

atgtccggta acagtgtcag tttcaggcgg cctgagcaca acttcttggg gaactctcct 780

aatataggtg gatcgtcgca atgcagtggg ggactcacct ttggtggtgg agctcggcaa 840

tgtacttcag ttggccggtg agaggtggaa ccaatcaaaa tcaagttcac tggtctggca 900

aatcatcctt ggcttagtca ctttactttc tgtgtttcat gtgttgttac ggaaatgttg 960

tcttttggaa gactctgcat tagcactcag acttttgcta gtgctttccc atgtattttg 1020

aaagttgctc ttgtttctgt tgttgaactg gaccagaaag tttgtgcttg aaaatttaac 1080

tttttaaaaa aaaaaaaaaa aaa 1103



68
210
PRT
Glycine max

68

Met Ala His Thr Ser Tyr Pro Cys Gly Asp Pro Tyr Phe Gly Ser Ser
1 5 10 15

Ile Val Ala Tyr Gly Thr Gln Ala Ile Thr Gln Gln Met Val Pro Gln
20 25 30

Met Leu Gly Leu Ala Ser Thr Arg Ile Ala Leu Pro Val Glu Leu Ala
35 40 45

Glu Asp Gly Pro Ile Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu
50 55 60

Arg Arg Arg Gln Ser Arg Ala Lys Leu Lys Ala Gln Asn Lys Leu Ile
65 70 75 80

Lys Ser Arg Lys Pro Tyr Leu His Glu Ser Arg His Arg His Ala Leu
85 90 95

Lys Arg Val Arg Gly Thr Gly Gly Arg Phe Leu Ser Ala Lys Gln Leu
100 105 110

Gln Gln Phe Asn Ala Glu Leu Val Thr Asp Ala His Ser Gly Pro Gly
115 120 125

Pro Val Asn Val Tyr Gln Lys Lys Asp Ala Ser Glu Ala Glu Ser His
130 135 140

Pro Ser Arg Thr Gly Lys Asn Ala Ser Ile Thr Phe Thr Ala Ile Ser
145 150 155 160

Gly Leu Thr Ser Met Ser Gly Asn Ser Val Ser Phe Arg Arg Pro Glu
165 170 175

His Asn Phe Leu Gly Asn Ser Pro Asn Ile Gly Gly Ser Ser Gln Cys
180 185 190

Ser Gly Gly Leu Thr Phe Gly Gly Gly Ala Arg Gln Cys Thr Ser Val
195 200 205

Gly Arg
210



69
1128
DNA
Glycine max

69

gcacgagggg tttgggtttc aagagaggag acatgcttaa cttcaaccca acacttcaag 60

tacttgcttc ttcataccct taccagatcc caaaggtcac gatctaattt taagtgatta 120

gtctgatgag cattttgaag gttacatgaa gcaatttctc tttttgaatc ttcctgacac 180

cgagatcaat tgttcacaag ttgattgcaa tcactcaatg gctcattctt cttatcccta 240

cggcgatcca attcttgctt atggaccaca agctattagt catccccaaa tggtacccca 300

gatgctggga ctagcatcca ccagagtggc attaccactt gatcttgctg aagatggacc 360

gatttatgtc aacgcgaaac aataccatgg tatactgaga aggcgacagt cacgagcaaa 420

acttgaggct cagaacaaac ttatcaaaag tcgtaagcca tatcttcatg agtctcggca 480

ccgccatgct ttgaataggg ttaggggatc tgggggtcga tttctgagta ccaaacagct 540

tgcacagtct aatgcagaat ttgtcaccgg tgcacattct ggttctgacc ctaccaacat 600

atatcagaaa gaacatccat tagaggtgga aagtcattcc tcaaaagatg gagataatgc 660

atcattcata acaacctact ccgaccggcc atgtttatct ggcaacaacc tcaattttcg 720

gcagcaggag tgcatgtttc tggggaattc tgcaaacatg agtggagcac cacagtgcag 780

tgggggactc acctttggcg gagcaaagca acgcacttca gttgtccggt gagagaagaa 840

actgatcgaa accgacttca ccggtcaggc aaatcatcct tggcttagtc acttttgtct 900

gtgtcttaat gtgttcgtac taaatgatca ttttgagaga ctcttcagtc tgcattagca 960

ctaataagac ctttccaatt gctttggcat gtattttaaa gttgctattg tactggattc 1020

tgaactggat tggaatagtc tgtgcatgga actagtatgt ttgtgttagt tactgttgaa 1080

tttccttctt taaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa 1128



70
228
PRT
Glycine max

70

Met Lys Gln Phe Leu Phe Leu Asn Leu Pro Asp Thr Glu Ile Asn Cys
1 5 10 15

Ser Gln Val Asp Cys Asn His Ser Met Ala His Ser Ser Tyr Pro Tyr
20 25 30

Gly Asp Pro Ile Leu Ala Tyr Gly Pro Gln Ala Ile Ser His Pro Gln
35 40 45

Met Val Pro Gln Met Leu Gly Leu Ala Ser Thr Arg Val Ala Leu Pro
50 55 60

Leu Asp Leu Ala Glu Asp Gly Pro Ile Tyr Val Asn Ala Lys Gln Tyr
65 70 75 80

His Gly Ile Leu Arg Arg Arg Gln Ser Arg Ala Lys Leu Glu Ala Gln
85 90 95

Asn Lys Leu Ile Lys Ser Arg Lys Pro Tyr Leu His Glu Ser Arg His
100 105 110

Arg His Ala Leu Asn Arg Val Arg Gly Ser Gly Gly Arg Phe Leu Ser
115 120 125

Thr Lys Gln Leu Ala Gln Ser Asn Ala Glu Phe Val Thr Gly Ala His
130 135 140

Ser Gly Ser Asp Pro Thr Asn Ile Tyr Gln Lys Glu His Pro Leu Glu
145 150 155 160

Val Glu Ser His Ser Ser Lys Asp Gly Asp Asn Ala Ser Phe Ile Thr
165 170 175

Thr Tyr Ser Asp Arg Pro Cys Leu Ser Gly Asn Asn Leu Asn Phe Arg
180 185 190

Gln Gln Glu Cys Met Phe Leu Gly Asn Ser Ala Asn Met Ser Gly Ala
195 200 205

Pro Gln Cys Ser Gly Gly Leu Thr Phe Gly Gly Ala Lys Gln Arg Thr
210 215 220

Ser Val Val Arg
225



71
1286
DNA
Helianthus sp.

71

gcacgagctt ctagattttc tctccgattc gtcgccccaa attttagggt ttttactttt 60

cgtcctctat actcgtagat cttggtgtaa cagtattgca taagtttcat gtcctcttct 120

gccatgcgag cgaattcatc tgattcgtct cctccagaac agtcgttaga cagggaatca 180

cagtctgatg aagttcttag tgaggaagaa gatgatgcaa gcaaagaaac acaaaatgct 240

tcgtcttttc gttcagataa aagttatcag cagcagggag taccaaatat ccttccaaat 300

aatggcgaaa ccgtagggca ggtcccacaa ctagaacttg tcggtcacac tattgcctgt 360

gctccaaatc cttattgtga tccatattat ggtggaatga tggcagctta tggtcagcct 420

tttgttcatc ctcagtttct tgagcaagca aggatgcctt tgccacttga aatggcgcaa 480

gagcctgttt acgtgaatgc caaacaatac catgcgatat taaggcgaag gcaatcccgt 540

gcaaaagcag agcttgagaa gaaacttata aaagacagaa agccttatct tcatgaatca 600

cggcatcagc atgctttgag aagggtaagg ggcaccggtg gtcgttttgc aaagaaaact 660

gacgttaata agaacacaac aggttcgggt tcaggttctg ccatgtcatc atcccagtcg 720

gtgaattcaa accgggtgca ctcagaatct gccgagagct tggacacacc aaggggtgga 780

ttggtaaatt cacacaatac tcgcacgtat cttgataacg gaggttcttt aggccagcag 840

tggataaaca tttcatctaa ccaatcttca cagagggctg ttgccatgaa gtgatgtcga 900

gtgtttaaca ccctttgtgt ctatccgtgg cttctaagct ggccggcaaa tcattcttgg 960

ctcatgttaa tatgagggac aaacaggtaa atgtaccttt tggtgtcctc tttggtttta 1020

ctttcaggat ttctttcttc ggaactgatg ttatgtacaa agtttgcttt tggggataga 1080

agaattggtt gggttgggtt tgtgtgttct tttctgaatg tttggtatat ttggaggtga 1140

agcatggagt ttaagatgtg cttatgtcta tcgtctaatt gtaggggcat atagtgctcc 1200

acagcctcca gcacatgtgt aatgtcgtgg ctgttgaaaa ttggagcttc atatttactg 1260

ttttgcaaaa aaaaaaaaaa aaaaaa 1286



72
261
PRT
Helianthus sp.

72

Met Ser Ser Ser Ala Met Arg Ala Asn Ser Ser Asp Ser Ser Pro Pro
1 5 10 15

Glu Gln Ser Leu Asp Arg Glu Ser Gln Ser Asp Glu Val Leu Ser Glu
20 25 30

Glu Glu Asp Asp Ala Ser Lys Glu Thr Gln Asn Ala Ser Ser Phe Arg
35 40 45

Ser Asp Lys Ser Tyr Gln Gln Gln Gly Val Pro Asn Ile Leu Pro Asn
50 55 60

Asn Gly Glu Thr Val Gly Gln Val Pro Gln Leu Glu Leu Val Gly His
65 70 75 80

Thr Ile Ala Cys Ala Pro Asn Pro Tyr Cys Asp Pro Tyr Tyr Gly Gly
85 90 95

Met Met Ala Ala Tyr Gly Gln Pro Phe Val His Pro Gln Phe Leu Glu
100 105 110

Gln Ala Arg Met Pro Leu Pro Leu Glu Met Ala Gln Glu Pro Val Tyr
115 120 125

Val Asn Ala Lys Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Ser Arg
130 135 140

Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys Asp Arg Lys Pro Tyr
145 150 155 160

Leu His Glu Ser Arg His Gln His Ala Leu Arg Arg Val Arg Gly Thr
165 170 175

Gly Gly Arg Phe Ala Lys Lys Thr Asp Val Asn Lys Asn Thr Thr Gly
180 185 190

Ser Gly Ser Gly Ser Ala Met Ser Ser Ser Gln Ser Val Asn Ser Asn
195 200 205

Arg Val His Ser Glu Ser Ala Glu Ser Leu Asp Thr Pro Arg Gly Gly
210 215 220

Leu Val Asn Ser His Asn Thr Arg Thr Tyr Leu Asp Asn Gly Gly Ser
225 230 235 240

Leu Gly Gln Gln Trp Ile Asn Ile Ser Ser Asn Gln Ser Ser Gln Arg
245 250 255

Ala Val Ala Met Lys
260



73
1306
DNA
Triticum aestivum

73

ggagaaacgg aaacagagac agagggagag gagacttgca gaggagagga gagaagaggc 60

ggaacaaggg aggagggagg ggtcgccgga agggggacat gctccctccg catctcacat 120

ctcgcagctt gaactgagag caagagcaga agcccatgag atgagacgca agcaaaatat 180

gcaagaaaat ggcacaatca tgattcagtt tggtcagcaa gtgcctaact gcgagtcctc 240

agctagcgat tctcctcaag aagtgtccgg aatgagcgaa gggagcttta atgagcagaa 300

tgatcaatct ggtaatcgcg atggctatac gaagagtagt gatgaaggca agatgatgtc 360

ggctttgtct ctgggcaatt cagaaatggc atacacaccg ccaaaacctg accgcactca 420

tccctttgcc atatcatacc catatgctga tccttactat ggtggtgcag tggcagccta 480

tggcgcacat gctattatgc acccccagat ggtgggcatg gtaccatcct ctcgagtgcc 540

actaccgatt gaaccagctg ccgccgaaga gcccatttat gtgaatgcga agcaatacca 600

tgccattctc cgaaggagac agctccgcgc aaaattagag gctgaaaata agctggtcaa 660

aagccgtaag ccgtacctgc atgagtcccg gcaccagcac gcgatgaagc gggctcgggg 720

aacaggcggg cggttcctca acgcaaagga gaagtctgaa gcttcaggcg gcggcaatgc 780

atcagcgagg tctggccacg ccggcgttcc cccggatggc ggcatgttct cgaagcacga 840

ccacacctta ccatccggtg acttccatta ccgcgcgaga gggggcgcct agggtgggca 900

cgcagttgcc ccctggcaaa tcatccttgg cttatgtgtg tggcgaatga ccgtcaactc 960

ggtccagtga tattgtaaaa ctgaatttag agtctgtgca attgtgttac ttgggggttt 1020

ggtagacagc ccttgtgttt ggggagggga cgatgcagct gcagctgcag ccggttctct 1080

tgttgtggta ggtttgtgtg gcatggcagg tgctgctaag ctggagcctg cttgaactgt 1140

tttcctgtca ctttgttgtt tggggtaata atgaccatct tgtatgatat tagtactgac 1200

ttggagtaag taataaccat tcccggcgtg atgcatttgc gcccgtggtg gtgtttctgt 1260

tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaa 1306



74
243
PRT
Triticum aestivum

74

Met Arg Arg Lys Gln Asn Met Gln Glu Asn Gly Thr Ile Met Ile Gln
1 5 10 15

Phe Gly Gln Gln Val Pro Asn Cys Glu Ser Ser Ala Ser Asp Ser Pro
20 25 30

Gln Glu Val Ser Gly Met Ser Glu Gly Ser Phe Asn Glu Gln Asn Asp
35 40 45

Gln Ser Gly Asn Arg Asp Gly Tyr Thr Lys Ser Ser Asp Glu Gly Lys
50 55 60

Met Met Ser Ala Leu Ser Leu Gly Asn Ser Glu Met Ala Tyr Thr Pro
65 70 75 80

Pro Lys Pro Asp Arg Thr His Pro Phe Ala Ile Ser Tyr Pro Tyr Ala
85 90 95

Asp Pro Tyr Tyr Gly Gly Ala Val Ala Ala Tyr Gly Ala His Ala Ile
100 105 110

Met His Pro Gln Met Val Gly Met Val Pro Ser Ser Arg Val Pro Leu
115 120 125

Pro Ile Glu Pro Ala Ala Ala Glu Glu Pro Ile Tyr Val Asn Ala Lys
130 135 140

Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu Glu
145 150 155 160

Ala Glu Asn Lys Leu Val Lys Ser Arg Lys Pro Tyr Leu His Glu Ser
165 170 175

Arg His Gln His Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg Phe
180 185 190

Leu Asn Ala Lys Glu Lys Ser Glu Ala Ser Gly Gly Gly Asn Ala Ser
195 200 205

Ala Arg Ser Gly His Ala Gly Val Pro Pro Asp Gly Gly Met Phe Ser
210 215 220

Lys His Asp His Thr Leu Pro Ser Gly Asp Phe His Tyr Arg Ala Arg
225 230 235 240

Gly Gly Ala



75
1077
DNA
Triticum aestivum

75

gcacgaggtt ggaaagtaac aaaccatgac ttctgtcacc gacggtgttt caggtgatca 60

tagagctgat gagcagcaga agcaagctgc tgctcaaggg aaccaggaag aggccccagc 120

tactagtata ggtagtcagg caatggtggc aacaccttcc acagattatg tcacacccta 180

tggccaccag gaagcttgcc atgcaatggg tcaaattgct tacccaactg tcgatccatt 240

ctatggaagc ctttatgcag cctacggtgg acaacctatg atgcatccac caatggtcgg 300

aatgcatgca gccgcaatac cgttgcctac tgatgcaatt gaagagcctg tgtatgtgaa 360

tgcaaagcaa tataatgcca tattaaggcg gcgccaatct cgggctaaag cagagtcaga 420

aaggaagctt atcaagggcc gcaagccata tctccatgag tcgcggcatc aacatgcctt 480

gaaaagggcc aggggagccg gaggccggtt tcttaacgca aagtcagacg acaatgaaga 540

gcattctgat tccagctcca aagataagca gaatggcgtt gcaccccgca gcagtggcca 600

atcctcccaa tctcccaaag gcgcgacttc ggctgataag tcagcaaacc atgaatgaga 660

tgctagaagg tccgccggac gcgacgatcc atgccaacag ttttgtacag tatatatatg 720

ctagtgagcg agagagagtc gcgccggcgg gtgccatagg atatatccgc tctgctctat 780

agtagtgata gacttatcga cagatttttt tgcagcattg gtccgtgttt gctcggtttg 840

gtttctacat tctgtacaat gagtagtttt ttttgtggtt tttgtgttcc ggggttagcc 900

gcgggtttgg tcaggaggct tttgtagctt ataaaagaag tataattagt gctacattgt 960

tttctttggt gtggatttgg tctcttagct gtgctgcatc ctcattcgtg gtgcagaaaa 1020

taatatctgg gtatacataa taatagctct gcctgcagct ttctttgcca aaaaaaa 1077



76
210
PRT
Triticum aestivum

76

Met Thr Ser Val Thr Asp Gly Val Ser Gly Asp His Arg Ala Asp Glu
1 5 10 15

Gln Gln Lys Gln Ala Ala Ala Gln Gly Asn Gln Glu Glu Ala Pro Ala
20 25 30

Thr Ser Ile Gly Ser Gln Ala Met Val Ala Thr Pro Ser Thr Asp Tyr
35 40 45

Val Thr Pro Tyr Gly His Gln Glu Ala Cys His Ala Met Gly Gln Ile
50 55 60

Ala Tyr Pro Thr Val Asp Pro Phe Tyr Gly Ser Leu Tyr Ala Ala Tyr
65 70 75 80

Gly Gly Gln Pro Met Met His Pro Pro Met Val Gly Met His Ala Ala
85 90 95

Ala Ile Pro Leu Pro Thr Asp Ala Ile Glu Glu Pro Val Tyr Val Asn
100 105 110

Ala Lys Gln Tyr Asn Ala Ile Leu Arg Arg Arg Gln Ser Arg Ala Lys
115 120 125

Ala Glu Ser Glu Arg Lys Leu Ile Lys Gly Arg Lys Pro Tyr Leu His
130 135 140

Glu Ser Arg His Gln His Ala Leu Lys Arg Ala Arg Gly Ala Gly Gly
145 150 155 160

Arg Phe Leu Asn Ala Lys Ser Asp Asp Asn Glu Glu His Ser Asp Ser
165 170 175

Ser Ser Lys Asp Lys Gln Asn Gly Val Ala Pro Arg Ser Ser Gly Gln
180 185 190

Ser Ser Gln Ser Pro Lys Gly Ala Thr Ser Ala Asp Lys Ser Ala Asn
195 200 205

His Glu
210



77
1378
DNA
Triticum aestivum

77

gcacgaggag attcccctct ccgcggcgca gacgaccacc cgccggccgc ccctgccgtc 60

gctctgctag gcagcgatga tgagcttcaa gggccacgac ggattcgggc aggcctccaa 120

tggtggtggt ggtggtggag cctccgtgcc atggtggacg gtgtcccaga tgctgtacgg 180

ggagccgggg gccgccttgt cgtcgtcgcc ggaggcggag cctcgccggg acgcccagtt 240

ccaggtcgtg cccagagctc agggcatcct ggatccactg ccggcgccca agagcggggc 300

tcctgaggtc ctcaagttct cggtgttcca agggaatttg gagtcgggag gcaacaaagg 360

agagaagccc atggagcact ccgccaccat cgcactgcag tcgccgctcc cggaatacaa 420

cagtcgcttc gaatttggcc cgggtccttc catgatgtct tctggttatc cttcagccga 480

gcagtgctat ggcctgctta ccacttacgc gatgaaatct acgcctggtg gccgattgct 540

cttgccactg aatgcaacag ctgacgcgcc gatttacgtg aatgcgaagc agtatgaagg 600

catccttcgc cgccgccgtg ctcgtgccaa ggtggagcga gagaatcagc tggtgaaagg 660

aagaaagccg tatcttcacg aatcacgcca ccgccacgcg atgcgccggg cgaggggcac 720

gggagggcgc ttcctcaaca ccaagaagga ggggaatggc aaggacgctg gaggaggagg 780

caagagggca gagtgcgccc cgcccacgcg cttcgccacg tctccgagct ccgtcatccc 840

gagcaacccg cactcccgga gcagcatctc gagcctctcc ggctcggagg tgtcgagcat 900

gtacgaccac gacgacgtgg accactacaa cagcatcgag cacctccgga cgcccttctt 960

caccccgctg ccgatcatca tggacggcga gcacggggca tccgccccct tcaagtgggc 1020

cacggccgcc gacggctgct gtgagctcct caaggcgtga cttgaggggg gtacacgcag 1080

gcacccagat caagagccgg ccatggccgg ctctggctcc gtctggttgt ctgcaggcaa 1140

atcattcttg gctctactgc attggggtgt ccttccacgt cgcattacct cttccctgag 1200

aactccggtg ctggttctca gggatcttgt gatgatgggg ctccccatat gcctgtaaaa 1260

tagtatcgga agcactagca gtgtactacg ggtatgaact ctgtggtact atcaggtatc 1320

tgtgtcagaa ctcagaataa gtatcaaact tcagggtcta aaaaaaaaaa aaaaaaaa 1378



78
327
PRT
Triticum aestivum

78

Met Met Ser Phe Lys Gly His Asp Gly Phe Gly Gln Ala Ser Asn Gly
1 5 10 15

Gly Gly Gly Gly Gly Ala Ser Val Pro Trp Trp Thr Val Ser Gln Met
20 25 30

Leu Tyr Gly Glu Pro Gly Ala Ala Leu Ser Ser Ser Pro Glu Ala Glu
35 40 45

Pro Arg Arg Asp Ala Gln Phe Gln Val Val Pro Arg Ala Gln Gly Ile
50 55 60

Leu Asp Pro Leu Pro Ala Pro Lys Ser Gly Ala Pro Glu Val Leu Lys
65 70 75 80

Phe Ser Val Phe Gln Gly Asn Leu Glu Ser Gly Gly Asn Lys Gly Glu
85 90 95

Lys Pro Met Glu His Ser Ala Thr Ile Ala Leu Gln Ser Pro Leu Pro
100 105 110

Glu Tyr Asn Ser Arg Phe Glu Phe Gly Pro Gly Pro Ser Met Met Ser
115 120 125

Ser Gly Tyr Pro Ser Ala Glu Gln Cys Tyr Gly Leu Leu Thr Thr Tyr
130 135 140

Ala Met Lys Ser Thr Pro Gly Gly Arg Leu Leu Leu Pro Leu Asn Ala
145 150 155 160

Thr Ala Asp Ala Pro Ile Tyr Val Asn Ala Lys Gln Tyr Glu Gly Ile
165 170 175

Leu Arg Arg Arg Arg Ala Arg Ala Lys Val Glu Arg Glu Asn Gln Leu
180 185 190

Val Lys Gly Arg Lys Pro Tyr Leu His Glu Ser Arg His Arg His Ala
195 200 205

Met Arg Arg Ala Arg Gly Thr Gly Gly Arg Phe Leu Asn Thr Lys Lys
210 215 220

Glu Gly Asn Gly Lys Asp Ala Gly Gly Gly Gly Lys Arg Ala Glu Cys
225 230 235 240

Ala Pro Pro Thr Arg Phe Ala Thr Ser Pro Ser Ser Val Ile Pro Ser
245 250 255

Asn Pro His Ser Arg Ser Ser Ile Ser Ser Leu Ser Gly Ser Glu Val
260 265 270

Ser Ser Met Tyr Asp His Asp Asp Val Asp His Tyr Asn Ser Ile Glu
275 280 285

His Leu Arg Thr Pro Phe Phe Thr Pro Leu Pro Ile Ile Met Asp Gly
290 295 300

Glu His Gly Ala Ser Ala Pro Phe Lys Trp Ala Thr Ala Ala Asp Gly
305 310 315 320

Cys Cys Glu Leu Leu Lys Ala
325



79
1192
DNA
Triticum aestivum

79

gcacgaggga gtgacgcggt cgaggagggg cgtgcggggg gcagacagag agggagcgca 60

aagggacggc ggaggcaagc tagcttcccg ggggcggacg caccgagaga gggcggcggg 120

agggaggagg cgcgtgggag ccatgcttct cccctcttct tcgtcttcct cctacgatcc 180

caaaggtgac tcctttggga aatcggttga cgatcatatg aggtcaactt tgacttttgg 240

tgataagcat tctgtatatg caggtcaaaa cactgactat ggccacccaa tggcttgcat 300

ttcatacccg ttcaacgatt ctggttctgg agtttgggcg gcctatgggt cacgggctat 360

gttccagccc ctcatggcgg gcggaggggc atctgcaacg gcaagagttc cattgcccgt 420

cgaactagca gcggatgagc ccatatttgt caatcccaaa caatataatg ggattctccg 480

gcgaaggcag ctgcgcgcta agttagaggc ccagaataaa ctcaccaaaa acagaaagcc 540

ctacctccac gagtcgcgcc atcttcacgc gatgaagcgg gcaagaggtt ccgggggacg 600

tttcctcaat tccaaacagc tgaagcagca gcagcagtct ggcagtgcct gcaccaaggc 660

cattgcggat ggcgcgaatt ccctgggttc gacccatcta cggctaggca gcggcgcagc 720

cggagaccga accaactcgg tgtccaaggc gatgtcctcc caagagaaca gcaagagagt 780

cgccgccccg gctcccgcct tcaccatgat tcaagcggcg cgcaaagacg acgacttctt 840

ccaccatcac gcccaccatc tcagcttctc cggtcatttt ggccagtcaa gcgaccgata 900

tacgtaataa ggggtcctcc gcgccccggt gtggtcaggc aactcatcct tggctttatt 960

tctggcgtgt taggacttca gagatagttt atctcacagt gctttgcagc ccatagttct 1020

cggcttgatg ttcggtatgc aaatgttggt gtactggtgc gttggaacaa aagtttgatg 1080

tgttcacatg acgattggtc gcggaactca tcttgtgttc tgctcgaccc taaaaaaaaa 1140

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ac 1192



80
254
PRT
Triticum aestivum

80

Met Leu Leu Pro Ser Ser Ser Ser Ser Ser Tyr Asp Pro Lys Gly Asp
1 5 10 15

Ser Phe Gly Lys Ser Val Asp Asp His Met Arg Ser Thr Leu Thr Phe
20 25 30

Gly Asp Lys His Ser Val Tyr Ala Gly Gln Asn Thr Asp Tyr Gly His
35 40 45

Pro Met Ala Cys Ile Ser Tyr Pro Phe Asn Asp Ser Gly Ser Gly Val
50 55 60

Trp Ala Ala Tyr Gly Ser Arg Ala Met Phe Gln Pro Leu Met Ala Gly
65 70 75 80

Gly Gly Ala Ser Ala Thr Ala Arg Val Pro Leu Pro Val Glu Leu Ala
85 90 95

Ala Asp Glu Pro Ile Phe Val Asn Pro Lys Gln Tyr Asn Gly Ile Leu
100 105 110

Arg Arg Arg Gln Leu Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Thr
115 120 125

Lys Asn Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu His Ala Met
130 135 140

Lys Arg Ala Arg Gly Ser Gly Gly Arg Phe Leu Asn Ser Lys Gln Leu
145 150 155 160

Lys Gln Gln Gln Gln Ser Gly Ser Ala Cys Thr Lys Ala Ile Ala Asp
165 170 175

Gly Ala Asn Ser Leu Gly Ser Thr His Leu Arg Leu Gly Ser Gly Ala
180 185 190

Ala Gly Asp Arg Thr Asn Ser Val Ser Lys Ala Met Ser Ser Gln Glu
195 200 205

Asn Ser Lys Arg Val Ala Ala Pro Ala Pro Ala Phe Thr Met Ile Gln
210 215 220

Ala Ala Arg Lys Asp Asp Asp Phe Phe His His His Ala His His Leu
225 230 235 240

Ser Phe Ser Gly His Phe Gly Gln Ser Ser Asp Arg Tyr Thr
245 250



81
1260
DNA
Triticum aestivum

81

gcacgagaag attatctctg taaactataa gttctgacag gtcttttgct ttattagtgg 60

ctcttctctc tgatgatgtt cacatcgccg aagccccatt tacagtgagg tgaattgatg 120

cgattatatc ttcatgctaa cagtaacacc ctttttgttt cagacaatga caatgatcat 180

gggaagcccg atcagcacat ggtaaagccg cttttatctt tggggaaccc agagactgtt 240

gctcccccac caatgcttga ttgtagccaa tcatttgcat atattcctta tactgctgat 300

gcttatgctg ggatctttcc aggatatgcc tcgcacgcta ttgttcatcc ccaattgaat 360

gctgcaacaa actctcgtgt gccgctccct gttgagcctg cagcagaaga gccaatgttt 420

gttaatgcaa agcagtacca tgcaattctt aggaggaggc agatacgtgc taaattggag 480

gcccaaaata agctggtgaa agcccggaag ccataccttc atgaatctcg gcaccgccat 540

gccatgaagc gagctcgtgg aacaggaggg cggttcctca acacaaagca actcgaggag 600

cagaagcaga agcaggcttc aggtggtgca agctgtacaa aggtccttgg caagaataca 660

ctccttcaga gtagccccgc cttcgcacct tcggcatcag ctccctccaa catgtcaagc 720

ttttcaacaa ccggcatgtt ggctaatcaa gagcgcacct gcttcccctc ggttggcttc 780

cgtcccacgg ttagcttcag tgcactgaat ggcaacggga agctggcccc aaacggcatg 840

caccagcgcg cttccatgat gaggtaaagc aaagcaccct ctggtgcgct gccggtggca 900

attcatcctt ggcttatgaa gatgttccgg aaatgtggtt gcaatatcag ctggaccaag 960

acattgttat gagtcctttt gagtttcatc tagttgaaag cactggtgtg ctgatgcaga 1020

ctgaaatctt catcacattt cttttgtgtg tacttattca aataaggcac accttgatta 1080

tcccagagac cggagttggg catggttgcg aaaccatagg cctatacttc cttacctgtt 1140

gtgaatgtat ctggtaatgt acttaagaga tggttgagcc tcgagctttg atgaatgctg 1200

ttgcagttca tcaactttgc aacctggttt gcctgatttc aaaaaaaaaa aaaaaaaaaa 1260



82
249
PRT
Triticum aestivum

82

Met Arg Leu Tyr Leu His Ala Asn Ser Asn Thr Leu Phe Val Ser Asp
1 5 10 15

Asn Asp Asn Asp His Gly Lys Pro Asp Gln His Met Val Lys Pro Leu
20 25 30

Leu Ser Leu Gly Asn Pro Glu Thr Val Ala Pro Pro Pro Met Leu Asp
35 40 45

Cys Ser Gln Ser Phe Ala Tyr Ile Pro Tyr Thr Ala Asp Ala Tyr Ala
50 55 60

Gly Ile Phe Pro Gly Tyr Ala Ser His Ala Ile Val His Pro Gln Leu
65 70 75 80

Asn Ala Ala Thr Asn Ser Arg Val Pro Leu Pro Val Glu Pro Ala Ala
85 90 95

Glu Glu Pro Met Phe Val Asn Ala Lys Gln Tyr His Ala Ile Leu Arg
100 105 110

Arg Arg Gln Ile Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Val Lys
115 120 125

Ala Arg Lys Pro Tyr Leu His Glu Ser Arg His Arg His Ala Met Lys
130 135 140

Arg Ala Arg Gly Thr Gly Gly Arg Phe Leu Asn Thr Lys Gln Leu Glu
145 150 155 160

Glu Gln Lys Gln Lys Gln Ala Ser Gly Gly Ala Ser Cys Thr Lys Val
165 170 175

Leu Gly Lys Asn Thr Leu Leu Gln Ser Ser Pro Ala Phe Ala Pro Ser
180 185 190

Ala Ser Ala Pro Ser Asn Met Ser Ser Phe Ser Thr Thr Gly Met Leu
195 200 205

Ala Asn Gln Glu Arg Thr Cys Phe Pro Ser Val Gly Phe Arg Pro Thr
210 215 220

Val Ser Phe Ser Ala Leu Asn Gly Asn Gly Lys Leu Ala Pro Asn Gly
225 230 235 240

Met His Gln Arg Ala Ser Met Met Arg
245



83
887
DNA
Canna edulis

83

gcacgagatt cactcccagt tcttctcccc ggttttccgc ctctctccgc aggttttcga 60

cgtctggttt gccctaaatc agctgaatgg atcagccgcc tggccacccc gccgtccctc 120

cggtgatggg cgtcgccgct ggagtgcctt atgcaactgc cgctgccgcc ggaccctatc 180

aggcctacca gaacctctac caccagcagc aacagcagca gcagcaacaa ctccagatgt 240

tctgggccga ccagtaccgt gagatcgagc aaactaccga cttccggaac cacagcctgc 300

cgctcgcgcg gatcaagaag atcatgaagg ccgacgagga cgtgcgtatg atcgctgccg 360

aggcgcctgt ggtgttcgcc cgcgcctgcg agatgttcat cctggaactc acccaccggt 420

cgtgggctca cgccgaggag aacaagcgcc ggacactgca gaagaacgat atagccgcgg 480

ccatcagccg caccgacgtg ttcgattttc tcattgatat cgtgccaagg gaggagggga 540

aggaagatgt tgcccacgcc ctcggacccc cagctggtgg tgaccccctc gcttactatt 600

atgtccagaa gtagaagctg ctgctgtgtg agtctttaat taaatgtctc catgttctca 660

atttcataaa tgccttagtg tgattataaa catagggcat ggggtttggt ttgttacctg 720

aagtgcactg aatttaatct ctagtgaact tgctttgcat agctggtgat gtgttcttgt 780

tagtaagttt atattgtttg ggtattgtcc atctaactac atgtatgctt atggcaagca 840

tcattacatt gatatggatg ggcatttacg ctgctctcat tcgcgcc 887



84
175
PRT
Canna edulis

84

Met Asp Gln Pro Pro Gly His Pro Ala Val Pro Pro Val Met Gly Val
1 5 10 15

Ala Ala Gly Val Pro Tyr Ala Thr Ala Ala Ala Ala Gly Pro Tyr Gln
20 25 30

Ala Tyr Gln Asn Leu Tyr His Gln Gln Gln Gln Gln Gln Gln Gln Gln
35 40 45

Leu Gln Met Phe Trp Ala Asp Gln Tyr Arg Glu Ile Glu Gln Thr Thr
50 55 60

Asp Phe Arg Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile Met
65 70 75 80

Lys Ala Asp Glu Asp Val Arg Met Ile Ala Ala Glu Ala Pro Val Val
85 90 95

Phe Ala Arg Ala Cys Glu Met Phe Ile Leu Glu Leu Thr His Arg Ser
100 105 110

Trp Ala His Ala Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp
115 120 125

Ile Ala Ala Ala Ile Ser Arg Thr Asp Val Phe Asp Phe Leu Ile Asp
130 135 140

Ile Val Pro Arg Glu Glu Gly Lys Glu Asp Val Ala His Ala Leu Gly
145 150 155 160

Pro Pro Ala Gly Gly Asp Pro Leu Ala Tyr Tyr Tyr Val Gln Lys
165 170 175



85
988
DNA
Vitis sp.

85

caaaaaaaaa atcccaaaac aagcagagac accctcctcc ctcgaatcaa attacaaaga 60

aatggagaac aaccagcagg cccaatcctc cccataccca ccacagcaac cctttcacca 120

tcttctgcag cagcaacagc agcagcttca gatgttttgg tcctaccaac gccaagagat 180

cgagcaggtg aacgacttca agaaccacca actgcctctg gcccgcatca agaagattat 240

gaaggcggat gaggatgtcc ggatgatctc ggcggaggcc ccaatcctct tcgccaaggc 300

ctgcgagctc ttcattctgg agctgacgat aaggtcgtgg ttgcacgcgg aggagaacaa 360

gaggaggaca ctgcagaaga atgatatcgc cgcggcgatt actaggacgg atatatttga 420

ttttttggtg gatattgtgc cgagggatga gatcaaggac gaggggggct tggggatggt 480

agggtcgacg gccagtgggg tgccgtacta ttatccgccg atggggcagc ccgcgccggg 540

agtaatgatg ggaaggccgg cggttccggg ggtggatccg ggggtgtacg tgcagccgcc 600

gtcgcaggca tggcagtcgg tgtggcagac ggcagaggac gggtcgtacg ggagcggagg 660

gagcagtgga caggggaatc ttgatggcca aggttaagca aacgcccatt gtggatgttg 720

tggtgcttcc cggcatgatg gaaactatcg agctcgtgga cagaacttgg attttccttg 780

gctatgaatt gctctgttat tatttgtgaa aactagttgg tttttaatgt aatggcttca 840

attagaaact tgttaaaaac cgtgatttgg accagtgcag tgatatgact caactaatcc 900

tatgtgcagt tctaaatgta aggtccatgt ttttcatttt aactgaatga ttctagttat 960

ctgattaaaa aaaaaaaaaa aaaaaaaa 988



86
211
PRT
Vitis sp.

86

Met Glu Asn Asn Gln Gln Ala Gln Ser Ser Pro Tyr Pro Pro Gln Gln
1 5 10 15

Pro Phe His His Leu Leu Gln Gln Gln Gln Gln Gln Leu Gln Met Phe
20 25 30

Trp Ser Tyr Gln Arg Gln Glu Ile Glu Gln Val Asn Asp Phe Lys Asn
35 40 45

His Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu
50 55 60

Asp Val Arg Met Ile Ser Ala Glu Ala Pro Ile Leu Phe Ala Lys Ala
65 70 75 80

Cys Glu Leu Phe Ile Leu Glu Leu Thr Ile Arg Ser Trp Leu His Ala
85 90 95

Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala Ala
100 105 110

Ile Thr Arg Thr Asp Ile Phe Asp Phe Leu Val Asp Ile Val Pro Arg
115 120 125

Asp Glu Ile Lys Asp Glu Gly Gly Leu Gly Met Val Gly Ser Thr Ala
130 135 140

Ser Gly Val Pro Tyr Tyr Tyr Pro Pro Met Gly Gln Pro Ala Pro Gly
145 150 155 160

Val Met Met Gly Arg Pro Ala Val Pro Gly Val Asp Pro Gly Val Tyr
165 170 175

Val Gln Pro Pro Ser Gln Ala Trp Gln Ser Val Trp Gln Thr Ala Glu
180 185 190

Asp Gly Ser Tyr Gly Ser Gly Gly Ser Ser Gly Gln Gly Asn Leu Asp
195 200 205

Gly Gln Gly
210



87
1572
DNA
Zea mays

87

ccacgcgtcc gcataagaaa aaaaatgaag cttgccattt cgctcagggc cctgcagcgg 60

cggcagctgg cgggagagag gcttgggact gggccgcccg gccgcgagga ataaactcac 120

tcctgtcttc atacgtatcc atagccggca ggcggcagta cctgtatgtg gttttagcta 180

tacgcgacct cagttcgggc gcaagctaca accccgacca ggcgagaaga agcatcgata 240

gtgtgacgag ctaacccacc accagcaacg taatccaaat ccatggacaa ccagccgctg 300

ccctactcca caggccagcc ccctgccccc ggaggagccc cggtggcggg catgcctggc 360

gcggccggcc tcccacccgt gccgcaccac cacctgctcc agcagcagca ggcccagctg 420

caggcgttct gggcgtacca gcgccaggag gcggagcgcg cgtccgcgtc ggacttcaag 480

aaccaccagc tgcctctggc ccggatcaag aagatcatga aggccgacga ggacgtgcgc 540

atgatctccg ccgaggcgcc cgtgctgttc gccaaggcct gcgagctctt catcctcgag 600

ctcactatcc gctcctggct ccacgccgag gagaacaagc gccgcaccct gcagcgcaac 660

gacgtcgccg cggccatcgc gcgcaccgac gtcttcgatt tcctcgtcga catcgtgccc 720

cgcgaggagg ccaaggagga gcccggcagc gccctcggct tcgcggcgcc tggtaccggc 780

gtcgtcgggg ctggcgcccc gggcggggcg ccagccgccg ggatgcccta ctactatccg 840

ccgatggggc agccggcgcc gatgatgccg gcctggcatg ttccggcctg ggacccggcc 900

tggcagcaag gggcagcgga tgtcgatcag agcggcagct tcagcgagga aggacaaggg 960

tttggagcag gccatggcgg cgccgctagc ttccctcctg cgcctccgac ctccgagtga 1020

tcgatcggcg cgtctcttgg tcctggcctc ctggcttagc tacatgtgca tgatgtcaat 1080

cgttcaatgt gccatgctgt gtatactcta cagcaaacgt ggtaatggag ctgctatgca 1140

tacagaacga ataaggcgtg acgtgtgaga ccgtaagagt acgtagtact aatatgtaga 1200

tgcacgtgac gtgccaatta atcaaagatt aacatgcagt taattaatta gtcctcctac 1260

cgaggtgcct catctatatt ttttttccat ttatatatcg agttcacaca atccataaga 1320

atacaaactt cggcaaggtt taggatttgg ggaacttgag gcttggggag ttagggttcc 1380

atggctaccg gtcgtgatga cacatggggc atcaaggtag attaagggtc tgtttgtttg 1440

aacttttaga gtttttttga aaagttgttg ttgaactttt gatactgaga agccaattca 1500

acgatgttat tagttcctga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1560

aaaaaaaaaa ag 1572



88
245
PRT
Zea mays

88

Met Asp Asn Gln Pro Leu Pro Tyr Ser Thr Gly Gln Pro Pro Ala Pro
1 5 10 15

Gly Gly Ala Pro Val Ala Gly Met Pro Gly Ala Ala Gly Leu Pro Pro
20 25 30

Val Pro His His His Leu Leu Gln Gln Gln Gln Ala Gln Leu Gln Ala
35 40 45

Phe Trp Ala Tyr Gln Arg Gln Glu Ala Glu Arg Ala Ser Ala Ser Asp
50 55 60

Phe Lys Asn His Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys
65 70 75 80

Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Leu Phe
85 90 95

Ala Lys Ala Cys Glu Leu Phe Ile Leu Glu Leu Thr Ile Arg Ser Trp
100 105 110

Leu His Ala Glu Glu Asn Lys Arg Arg Thr Leu Gln Arg Asn Asp Val
115 120 125

Ala Ala Ala Ile Ala Arg Thr Asp Val Phe Asp Phe Leu Val Asp Ile
130 135 140

Val Pro Arg Glu Glu Ala Lys Glu Glu Pro Gly Ser Ala Leu Gly Phe
145 150 155 160

Ala Ala Pro Gly Thr Gly Val Val Gly Ala Gly Ala Pro Gly Gly Ala
165 170 175

Pro Ala Ala Gly Met Pro Tyr Tyr Tyr Pro Pro Met Gly Gln Pro Ala
180 185 190

Pro Met Met Pro Ala Trp His Val Pro Ala Trp Asp Pro Ala Trp Gln
195 200 205

Gln Gly Ala Ala Asp Val Asp Gln Ser Gly Ser Phe Ser Glu Glu Gly
210 215 220

Gln Gly Phe Gly Ala Gly His Gly Gly Ala Ala Ser Phe Pro Pro Ala
225 230 235 240

Pro Pro Thr Ser Glu
245



89
1164
DNA
Zea mays

89

gcacgagtct ccccccattc tccaatccgt gccctagtcg agccagccgc gaggaaggag 60

gcgtctcgcc tagcgcccgc ccgtcggccg accttctgct gcaccttcga actctggaaa 120

gatcatagat ttttgggcaa tagcaagtgg acatggaacc atcctctcag cctcagcctg 180

cgatgggtgt cgccgccggt gggtcacaag tgtatcctgc gtctgcctac ccgcctgcag 240

caacagtagc tcctcctgct gttgcatctg ctggtttaca gtcagtgcaa ccattcccag 300

ccaaccctgc ccatatgagt gctcagcacc agattgtcta ccaacaagct caacagttcc 360

accaacagct ccagcagcag caacagcagc agcttcagca gttctgggtc gaacgcatga 420

ctgaaatcga ggcaacagct gatttcagga accacaactt gccacttgcg aggataaaga 480

agatcatgaa ggccgacgaa gatgtccgca tgatctcagc cgaagctccc gtggtcttcg 540

caaaagcttg cgagatattc atactggagc tgacgctgag gtcgtggatg cacaccgagg 600

agaacaagcg ccgcaccttg cagaagaacg acattgccgc agccatcacc aggaccgaca 660

tttacgactt cttggtcgac attgttccca gggatgagat gaaggacgac ggaatcgggc 720

ttcctaggcc cgggctgcca cccatgggag ccccagctga cgcatatcca tactactaca 780

tgccacagca gcaggtgcct ggtcctggga tggtttatgg cgcccagcaa ggccacccgg 840

tgacgtatct gtggcaggat cctcaggaac agcaggagca agctcctgaa gagcagcagt 900

ctctgcatga aagggactga ggatgtcgct caagctatca cctgattttt cagagctctc 960

attttaggtt ctctaaactg caggttttcg ttggctaata tcgttgggta tcaaactgaa 1020

acaggtaggg tgtagcatca tggtagtttg atttctgctg tggtgttagt tggagggata 1080

atgattagcg gctagtggat taaagttacc cataccgttt cctttcgttc caaaaaaaaa 1140

aaaaaaaaaa aaaaaaaaaa aaaa 1164



90
255
PRT
Zea mays

90

Met Glu Pro Ser Ser Gln Pro Gln Pro Ala Met Gly Val Ala Ala Gly
1 5 10 15

Gly Ser Gln Val Tyr Pro Ala Ser Ala Tyr Pro Pro Ala Ala Thr Val
20 25 30

Ala Pro Pro Ala Val Ala Ser Ala Gly Leu Gln Ser Val Gln Pro Phe
35 40 45

Pro Ala Asn Pro Ala His Met Ser Ala Gln His Gln Ile Val Tyr Gln
50 55 60

Gln Ala Gln Gln Phe His Gln Gln Leu Gln Gln Gln Gln Gln Gln Gln
65 70 75 80

Leu Gln Gln Phe Trp Val Glu Arg Met Thr Glu Ile Glu Ala Thr Ala
85 90 95

Asp Phe Arg Asn His Asn Leu Pro Leu Ala Arg Ile Lys Lys Ile Met
100 105 110

Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Val
115 120 125

Phe Ala Lys Ala Cys Glu Ile Phe Ile Leu Glu Leu Thr Leu Arg Ser
130 135 140

Trp Met His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp
145 150 155 160

Ile Ala Ala Ala Ile Thr Arg Thr Asp Ile Tyr Asp Phe Leu Val Asp
165 170 175

Ile Val Pro Arg Asp Glu Met Lys Asp Asp Gly Ile Gly Leu Pro Arg
180 185 190

Pro Gly Leu Pro Pro Met Gly Ala Pro Ala Asp Ala Tyr Pro Tyr Tyr
195 200 205

Tyr Met Pro Gln Gln Gln Val Pro Gly Pro Gly Met Val Tyr Gly Ala
210 215 220

Gln Gln Gly His Pro Val Thr Tyr Leu Trp Gln Asp Pro Gln Glu Gln
225 230 235 240

Gln Glu Gln Ala Pro Glu Glu Gln Gln Ser Leu His Glu Arg Asp
245 250 255



91
1270
DNA
Zea mays

91

gcacgaggac gagacagaga gagaaggcca agaggcttcc tctccccatt cctcccttcc 60

gtgccctagc cgagccagcc gcgaggaagg aggcatcccg ccgtctcgcc tggcgcccgc 120

ccgtcggccg accttctgcc gcagcttcca attgtaaaaa gatcatagat ttttgtgcaa 180

gagcgagtgg atatggaacc atcccctcag cctatgggtg tcgctgccgg tgggtcacaa 240

gtgtatcctg cctctgccta tccgcctgca gcaacagtag ctcctgcttc tgttgtatct 300

gctggtttac agtcagggca gccattccca gccaatcctg gtcatatgag tgctcagcac 360

cagattgtct accaacaagc tcaacaattc caccaacagc tccagcagca acaacaacag 420

cagcttcagc agttctgggt tgaacgcatg actgaaattg aggcgacgac tgatttcaag 480

aaccacaact tgccacttgc gaggataaag aagatcatga aggccgatga agatgttcgc 540

atgatctcag ctgaagctcc tgtagtcttt gcaaaagctt gtgagatatt catactggag 600

ctgacactta ggtcgtggat gcacactgag gagaacaagc gccgcacctt gcaaaagaat 660

gacattgcag cagcgatcac taggactgac atttatgact tcttggtcga cattgttccc 720

agggatgaga tgaaggagga cggaattggg cttcctaggg ctggtctgcc acccatggga 780

gccccagctg atgcatatcc atactactac atgccacagc agcaggtgcc tggttctgga 840

atggtttatg gtgcccagca agggcaccca gtgacttatt tgtggcagga gcctcagcaa 900

cagcaggagc aagctcctga agagcagcaa tctgcatgaa agtggctgag aatattgctc 960

agaagctatc acctgattca gagttctcat tttaggttgt ccaaactgca ggttttctta 1020

gtaatatcgt tggttatcaa actgaaacag gcgattctaa gtagggtgta gcatcatggt 1080

agtttcattt ctgcttgtga tgttagttga aaggataatg attagtggct agtggattaa 1140

agttaccata ccatttcctt ctattccgaa agtttgcctc catgaggcct ctgatatgac 1200

gtgctagttg ttaatgcttc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1260

aaaaaaaaaa 1270



92
248
PRT
Zea mays

92

Met Glu Pro Ser Pro Gln Pro Met Gly Val Ala Ala Gly Gly Ser Gln
1 5 10 15

Val Tyr Pro Ala Ser Ala Tyr Pro Pro Ala Ala Thr Val Ala Pro Ala
20 25 30

Ser Val Val Ser Ala Gly Leu Gln Ser Gly Gln Pro Phe Pro Ala Asn
35 40 45

Pro Gly His Met Ser Ala Gln His Gln Ile Val Tyr Gln Gln Ala Gln
50 55 60

Gln Phe His Gln Gln Leu Gln Gln Gln Gln Gln Gln Gln Leu Gln Gln
65 70 75 80

Phe Trp Val Glu Arg Met Thr Glu Ile Glu Ala Thr Thr Asp Phe Lys
85 90 95

Asn His Asn Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp
100 105 110

Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Val Phe Ala Lys
115 120 125

Ala Cys Glu Ile Phe Ile Leu Glu Leu Thr Leu Arg Ser Trp Met His
130 135 140

Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala
145 150 155 160

Ala Ile Thr Arg Thr Asp Ile Tyr Asp Phe Leu Val Asp Ile Val Pro
165 170 175

Arg Asp Glu Met Lys Glu Asp Gly Ile Gly Leu Pro Arg Ala Gly Leu
180 185 190

Pro Pro Met Gly Ala Pro Ala Asp Ala Tyr Pro Tyr Tyr Tyr Met Pro
195 200 205

Gln Gln Gln Val Pro Gly Ser Gly Met Val Tyr Gly Ala Gln Gln Gly
210 215 220

His Pro Val Thr Tyr Leu Trp Gln Glu Pro Gln Gln Gln Gln Glu Gln
225 230 235 240

Ala Pro Glu Glu Gln Gln Ser Ala
245



93
511
DNA
Zea mays

unsure
(442)
n = A, C, G, or T


93

gactcaactc agtgctcagc accagatggt gtaccagcag gctcagcaat ttcatcaaca 60

acttcagcaa cagcaggaac aacagctcag ggagttctgg actacccaga tggatgagat 120

caagcaagca aatgacttca agatccacac cttgccactt gcaaggataa agaagataat 180

gaaggctgat gaggatgtgc ggatgatctc tgcagaagct cctgttgtgt ttgcgaaggc 240

atgcgaggta ttcatattag agctgacatt gaggtcatgg atgcacacag aggagaacaa 300

gcgccggacc ttgcagaaga acgacattgc agctgccatc accaggactg atatatatga 360

cttcttggtg gacataatcc cgagggatga aatgaaagag gaagggcttc ggacataatc 420

ccatagttgg cctgccgcct gntatggggg cntccagctt gatcatgggt cttnatccat 480

tattactatg tggccantta acangtgcca a 511



94
135
PRT
Zea mays

94

Thr Gln Leu Ser Ala Gln His Gln Met Val Tyr Gln Gln Ala Gln Gln
1 5 10 15

Phe His Gln Gln Leu Gln Gln Gln Gln Glu Gln Gln Leu Arg Glu Phe
20 25 30

Trp Thr Thr Gln Met Asp Glu Ile Lys Gln Ala Asn Asp Phe Lys Ile
35 40 45

His Thr Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu
50 55 60

Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Val Phe Ala Lys Ala
65 70 75 80

Cys Glu Val Phe Ile Leu Glu Leu Thr Leu Arg Ser Trp Met His Thr
85 90 95

Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Cys Ser Cys
100 105 110

His His Pro Gly Leu Ile Tyr Met Thr Ser Leu Val Asp Ile Ile Pro
115 120 125

Arg Asp Glu Met Lys Glu Glu
130 135



95
499
DNA
Zea mays

unsure
(278)
n = A, C, G, or T


95

ctttctcccc tgttgttgtt gatccaaaaa gccacctccc cccaacccaa tcccgtcgtc 60

actctctcac tccactgcct ccggaacacc ctagcaatgg atcccaactc cagcatccct 120

cccccggtga tgggcgcggc ggtggcgtac cctccggcgg ccggcgccgc gtactccgcc 180

gggccgtacg cgcacgcgca cgcggcgttg ggcgcgctgt acccgcctcc cccggcgccg 240

ggtcccccct cctcgcacca gggcggcgcg gcggcggngc agctgcagct gttctgggcg 300

gagcagtacc gcgagatcga ggcgacgacg gacttcaaga accacaacct gccgctgggc 360

cgcatcanga agatcatgaa ggcggacgan ngactgcgca tgatcgccgc cgaggcgccg 420

gtggtgttcg cccgcgcctg cgagatgttc ancctggagc tgaccaancg cggntgggcn 480

cacgcngagg aaaaaaaac 499



96
134
PRT
Zea mays

UNSURE
(61)
Xaa = any amino acid


96

Met Asp Pro Asn Ser Ser Ile Pro Pro Pro Val Met Gly Ala Ala Val
1 5 10 15

Ala Tyr Pro Pro Ala Ala Gly Ala Ala Tyr Ser Ala Gly Pro Tyr Ala
20 25 30

His Ala His Ala Ala Leu Gly Ala Leu Tyr Pro Pro Pro Pro Ala Pro
35 40 45

Gly Pro Pro Ser Ser His Gln Gly Gly Ala Ala Ala Xaa Gln Leu Gln
50 55 60

Leu Phe Trp Ala Glu Gln Tyr Arg Glu Ile Glu Ala Thr Thr Asp Phe
65 70 75 80

Lys Asn His Asn Leu Pro Leu Gly Arg Ile Xaa Lys Ile Met Lys Ala
85 90 95

Asp Xaa Xaa Leu Arg Met Ile Ala Ala Glu Ala Pro Val Val Phe Ala
100 105 110

Arg Ala Cys Glu Met Phe Xaa Leu Glu Leu Thr Xaa Arg Gly Trp Ala
115 120 125

His Ala Glu Glu Lys Lys
130



97
1060
DNA
Zea mays

97

gcacgagaag caccttcctc ttcctcttcc tccgcccccc aatccccctc gtctcacaac 60

cctagctgcc cccgaatcca tggatcccaa caaatccagc accccgccgc cgcctccagt 120

catgggtgcc cccgttgcct accctccgcc ggcgtaccct cccggtgtgg ccgccggcgc 180

cggcgcctac ccgccgcagc tctacgcgcc gccggctgct gccgcggccc agcaggcggc 240

ggccgcgcag cagcagcagc tgcagatatt ctgggcggag cagtaccgcg agatcgaggc 300

cactaccgac ttcaagaatc acaacctccc gctcgcccgc atcaagaaga tcatgaaagc 360

cgacgaggac gtccgcatga tcgccgccga ggctcccgtg gtgttcgccc gggcctgcga 420

gatgttcatc ctcgagctca cccatcgcgg ctgggcgcac gccgaagaga acaagcgccg 480

cacgctccag aaatccgaca ttgccgctgc catcgcccgc accgaggtat tcgacttcct 540

tgtggacatc gttccgcgcg acgacggtaa agacgctgat gcggcggccg ccgcagctgc 600

cgcggctgcc gggatcccgc gccccgccgc cggagtacca gccaccgacc ctctcgccta 660

ctactacgtg cctcagcagt aatgtatcat catcacgtta ttgttccgtc tatgtgcctg 720

agcaataatg tatcatcatt gccttattgt tccggggcag ttgtgttatt tgtgtctgtt 780

tagttgctgc tgctgttacc gcgtaatagc atatgtgtta tctgtgtctg tttagttgct 840

gctgctgttg ccgcgtaata aaacttggtc gtttacgggg ctccctcaag attaagaatt 900

gagttgtttg atggtagaat cctggtaagg ttgttgtaac tggggggcgc ctttgtttgg 960

gctggtagtg tatgcctagg cctcacttat ctgatgctgt aatgcgacaa gtattatgtg 1020

gttgtctggt aattattgtg caaaaaaaaa aaaaaaaaaa 1060



98
200
PRT
Zea mays

98

Met Asp Pro Asn Lys Ser Ser Thr Pro Pro Pro Pro Pro Val Met Gly
1 5 10 15

Ala Pro Val Ala Tyr Pro Pro Pro Ala Tyr Pro Pro Gly Val Ala Ala
20 25 30

Gly Ala Gly Ala Tyr Pro Pro Gln Leu Tyr Ala Pro Pro Ala Ala Ala
35 40 45

Ala Ala Gln Gln Ala Ala Ala Ala Gln Gln Gln Gln Leu Gln Ile Phe
50 55 60

Trp Ala Glu Gln Tyr Arg Glu Ile Glu Ala Thr Thr Asp Phe Lys Asn
65 70 75 80

His Asn Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu
85 90 95

Asp Val Arg Met Ile Ala Ala Glu Ala Pro Val Val Phe Ala Arg Ala
100 105 110

Cys Glu Met Phe Ile Leu Glu Leu Thr His Arg Gly Trp Ala His Ala
115 120 125

Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Ser Asp Ile Ala Ala Ala
130 135 140

Ile Ala Arg Thr Glu Val Phe Asp Phe Leu Val Asp Ile Val Pro Arg
145 150 155 160

Asp Asp Gly Lys Asp Ala Asp Ala Ala Ala Ala Ala Ala Ala Ala Ala
165 170 175

Ala Gly Ile Pro Arg Pro Ala Ala Gly Val Pro Ala Thr Asp Pro Leu
180 185 190

Ala Tyr Tyr Tyr Val Pro Gln Gln
195 200



99
901
DNA
Zea mays

99

gcacgagtga ccgccggaac accctaggca atggagccca aatccaccac ccctcccccg 60

ccccccgtga tgggcgcgcc catcgcgtat cctcccccgc ccggcgccgc gtaccccgcc 120

gggccgtacg tgcacgcgcc ggcggccgcg ctctaccctc ctcctcccct gccgccggcg 180

cccccctcct cgcagcaggg cgccgcggcg gcgcaccagc agcagctatt ctgggcggag 240

caataccgcg agatcgaggc caccaccgac ttcaagaacc acaacctgcc gctcgcccgc 300

atcaagaaga tcatgaaggc cgacgaggac gtgcgcatga tcgccgccga ggcgcccgtc 360

gtcttctccc gcgcctgcga gatgttcatc ctcgagctca cccaccgcgg ctgggcacac 420

gccgaggaga acaagcgccg cacgctgcag aagtccgaca tcgccgccgc cgtcgcgcgc 480

accgaggtct tcgacttcct cgtcgacatc gtgccgcggg acgaggccaa ggacgccgac 540

tccgccgcca tgggagcagc cgggatcccg caccccgccg ccggcctgcc cgccgccgat 600

cccatgggct actactacgt ccagccgcag taacgaattt gcttccttat catggtttcg 660

cttccatgca gcctttgcgg gtttttagta aactattatt attactgaga gtgccctgtt 720

gttacccatg ctctgttgtt gccacccaat aactcgatga cctgatgatc atctgatgtg 780

cctcccgttc cgtaacaagt gattccattt ctgattaaaa aaaaaaaaaa aaaaaaaaaa 840

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaccaa aaaaaaaaaa aaaaaaaaaa 900

a 901



100
200
PRT
Zea mays

100

Met Glu Pro Lys Ser Thr Thr Pro Pro Pro Pro Pro Val Met Gly Ala
1 5 10 15

Pro Ile Ala Tyr Pro Pro Pro Pro Gly Ala Ala Tyr Pro Ala Gly Pro
20 25 30

Tyr Val His Ala Pro Ala Ala Ala Leu Tyr Pro Pro Pro Pro Leu Pro
35 40 45

Pro Ala Pro Pro Ser Ser Gln Gln Gly Ala Ala Ala Ala His Gln Gln
50 55 60

Gln Leu Phe Trp Ala Glu Gln Tyr Arg Glu Ile Glu Ala Thr Thr Asp
65 70 75 80

Phe Lys Asn His Asn Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys
85 90 95

Ala Asp Glu Asp Val Arg Met Ile Ala Ala Glu Ala Pro Val Val Phe
100 105 110

Ser Arg Ala Cys Glu Met Phe Ile Leu Glu Leu Thr His Arg Gly Trp
115 120 125

Ala His Ala Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Ser Asp Ile
130 135 140

Ala Ala Ala Val Ala Arg Thr Glu Val Phe Asp Phe Leu Val Asp Ile
145 150 155 160

Val Pro Arg Asp Glu Ala Lys Asp Ala Asp Ser Ala Ala Met Gly Ala
165 170 175

Ala Gly Ile Pro His Pro Ala Ala Gly Leu Pro Ala Ala Asp Pro Met
180 185 190

Gly Tyr Tyr Tyr Val Gln Pro Gln
195 200



101
1118
DNA
Oryza sativa

101

cacacacagc tacaaatcga ctgtaattaa ggtacgtata tataggtgac aatggacaac 60

cagcagctac cctacgccgg tcagccggcg gccgcaggcg ccggagcccc ggtgccgggc 120

gtgcctggcg cgggcgggcc gccggcggtg ccgcaccacc acctgctcca gcagcagcag 180

gcgcagctgc aggcgttctg ggcgtaccag cggcaggagg cggagcgcgc gtcggcgtcg 240

gacttcaaga accaccagct gccgctggcg cggatcaaga agatcatgaa ggcggacgag 300

gacgtgcgca tgatctcggc ggaggcgccc gtgctgttcg ccaaggcgtg cgagctcttc 360

atcctggagc tcaccatccg ctcgtggctg cacgccgagg agaacaagcg ccgcaccctg 420

cagcgcaacg acgtcgccgc cgccatcgcg cgcaccgacg tgttcgactt cctcgtcgac 480

atcgtgccgc gggaggaggc caaggaggag cccggcagcg cgctcgggtt cgcggcggga 540

gggcccgccg gcgccgttgg agcggccggc cccgccgcgg ggctgccgta ctactacccg 600

ccgatggggc agccggcgcc gatgatgccg gcgtggcatg ttccggcgtg ggacccggcg 660

tggcagcaag gagcagcgcc ggatgtggac cagggcgccg ccggcagctt cagcgaggaa 720

gggcagcaag gttttgcagg ccatggcggt gcggcagcta gcttccctcc tgcacctcca 780

agctccgaat agtgatgatc catatggttc catgcatgca tcgctgaggt gctagctagc 840

tactatagct gctcaaatca aatgctcaat gtgtcggtaa ttaattaatg tggtacgtat 900

taacttaacc gatgtacgta atggacgctc aagctaatta agggatgtac aatttactaa 960

ttaatttaat ttgtaatata tagccgatta actagcaagg tgacccagta ctatttgtaa 1020

tttcttttcc cgttatgcta ctaattgtgg acgcacaaac cattaccgga acagaaatta 1080

ctactgatga attactataa aaaaaaaaaa aaaaaaaa 1118



102
246
PRT
Oryza sativa

102

Met Asp Asn Gln Gln Leu Pro Tyr Ala Gly Gln Pro Ala Ala Ala Gly
1 5 10 15

Ala Gly Ala Pro Val Pro Gly Val Pro Gly Ala Gly Gly Pro Pro Ala
20 25 30

Val Pro His His His Leu Leu Gln Gln Gln Gln Ala Gln Leu Gln Ala
35 40 45

Phe Trp Ala Tyr Gln Arg Gln Glu Ala Glu Arg Ala Ser Ala Ser Asp
50 55 60

Phe Lys Asn His Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys
65 70 75 80

Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Leu Phe
85 90 95

Ala Lys Ala Cys Glu Leu Phe Ile Leu Glu Leu Thr Ile Arg Ser Trp
100 105 110

Leu His Ala Glu Glu Asn Lys Arg Arg Thr Leu Gln Arg Asn Asp Val
115 120 125

Ala Ala Ala Ile Ala Arg Thr Asp Val Phe Asp Phe Leu Val Asp Ile
130 135 140

Val Pro Arg Glu Glu Ala Lys Glu Glu Pro Gly Ser Ala Leu Gly Phe
145 150 155 160

Ala Ala Gly Gly Pro Ala Gly Ala Val Gly Ala Ala Gly Pro Ala Ala
165 170 175

Gly Leu Pro Tyr Tyr Tyr Pro Pro Met Gly Gln Pro Ala Pro Met Met
180 185 190

Pro Ala Trp His Val Pro Ala Trp Asp Pro Ala Trp Gln Gln Gly Ala
195 200 205

Ala Pro Asp Val Asp Gln Gly Ala Ala Gly Ser Phe Ser Glu Glu Gly
210 215 220

Gln Gln Gly Phe Ala Gly His Gly Gly Ala Ala Ala Ser Phe Pro Pro
225 230 235 240

Ala Pro Pro Ser Ser Glu
245



103
1343
DNA
Oryza sativa

103

tctgacccaa gggcgaccgc gtctccctct ctctctctct ctccgccgcc gacgccgagg 60

gctccacgag agggaggtgg gcggcgcggc ccttcgccgg agggagcgct ctccgccgcc 120

gccgctcccg ctcccgccgg cgcgggagat ccgggcgtcg tctctcgggc ctttggcttt 180

ggacggacaa gagctgacat ggaaccatcc tcacagcctc agcctgtgat gggtgttgcc 240

actggtgggt cacaagcata tcctcctcct gctgctgcat atccacctca agccatggtt 300

cctggagctc ctgctgttgt tcctcctggc tcacagccat cagcaccatt ccccactaat 360

ccagctcaac tcagtgctca gcaccagcta gtctaccaac aagcccagca atttcatcag 420

cagctgcagc aacagcaaca gcagcaactc cgtgagttct gggctaacca aatggaagag 480

attgagcaaa caaccgactt caagaaccac agcttgccac tcgcaaggat aaagaagata 540

atgaaggctg atgaggatgt ccggatgatc tcggcagaag cccccgttgt cttcgcaaag 600

gcatgcgagg tattcatatt agagttaaca ttgaggtcgt ggatgcacac ggaggagaac 660

aagcgccgga ccttgcagaa gaatgacatt gcagctgcca tcaccaggac tgatatctat 720

gacttcttgg tggacatagt tcccagggat gaaatgaaag aagaagggct tgggcttccg 780

agggttggcc taccgcctaa tgtggggggc gcagcagaca catatccata ttactacgtg 840

ccagcgcagc aggggcctgg atcaggaatg atgtacggtg gacagcaagg tcacccggtg 900

acgtatgtgt ggcagcagcc tcaagagcaa caggaagagg cccctgaaga gcagcactct 960

ctgccagaaa gtagctaaag atgatacagt gaagttgtga cattgatata cattgtcctg 1020

tgaacttagg gcctctaaaa ctcagtgctc ttgtcaaaac tattcccatg attgttggct 1080

gaaacgggta atctgattag gtcttaggct ttcctaatgt tagttctgct ctgctatggc 1140

agcagtagaa aaaaaaaaga ttgtgatttg gtaggtgatt tgcaactaat gtagtaactg 1200

taccttacct ttcatcagtt tctaatccaa tactcaaaag tgctggcatg tggagaccct 1260

tgtatgaatt gagtgtttgt tcatgtcatg catcagtctg ttgcctcatt tatcagtcat 1320

catgcctcct gctttgcaaa aaa 1343



104
259
PRT
Oryza sativa

104

Met Glu Pro Ser Ser Gln Pro Gln Pro Val Met Gly Val Ala Thr Gly
1 5 10 15

Gly Ser Gln Ala Tyr Pro Pro Pro Ala Ala Ala Tyr Pro Pro Gln Ala
20 25 30

Met Val Pro Gly Ala Pro Ala Val Val Pro Pro Gly Ser Gln Pro Ser
35 40 45

Ala Pro Phe Pro Thr Asn Pro Ala Gln Leu Ser Ala Gln His Gln Leu
50 55 60

Val Tyr Gln Gln Ala Gln Gln Phe His Gln Gln Leu Gln Gln Gln Gln
65 70 75 80

Gln Gln Gln Leu Arg Glu Phe Trp Ala Asn Gln Met Glu Glu Ile Glu
85 90 95

Gln Thr Thr Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile Lys
100 105 110

Lys Ile Met Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala
115 120 125

Pro Val Val Phe Ala Lys Ala Cys Glu Val Phe Ile Leu Glu Leu Thr
130 135 140

Leu Arg Ser Trp Met His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln
145 150 155 160

Lys Asn Asp Ile Ala Ala Ala Ile Thr Arg Thr Asp Ile Tyr Asp Phe
165 170 175

Leu Val Asp Ile Val Pro Arg Asp Glu Met Lys Glu Glu Gly Leu Gly
180 185 190

Leu Pro Arg Val Gly Leu Pro Pro Asn Val Gly Gly Ala Ala Asp Thr
195 200 205

Tyr Pro Tyr Tyr Tyr Val Pro Ala Gln Gln Gly Pro Gly Ser Gly Met
210 215 220

Met Tyr Gly Gly Gln Gln Gly His Pro Val Thr Tyr Val Trp Gln Gln
225 230 235 240

Pro Gln Glu Gln Gln Glu Glu Ala Pro Glu Glu Gln His Ser Leu Pro
245 250 255

Glu Ser Ser



105
1085
DNA
Oryza sativa

105

gcacgagaag gaatctacgt tgcatgcata agacgtgttg gaaatatcat aagttttggg 60

acaagcaaga gaggacatgg agccatcatc acaacctcag ccggcaattg gtgttgttgc 120

tggtggatca caagtgtacc ctgcataccg gcctgcagca acagtgccta cagctcctgc 180

tgtcattcct gccggttcac agccagcacc gtcgttccct gccaaccctg atcaactgag 240

tgctcagcac cagctcgtct atcagcaagc ccagcaattt caccagcagc ttcagcagca 300

gcaacagcgt caactccagc agttttgggc tgaacgtctg gtcgatattg aacaaactac 360

tgacttcaag aaccacagct tgccacttgc taggataaag aagatcatga aggcagatga 420

ggacgttcgc atgatctccg cagaggctcc tgtgatcttt gcgaaagcat gtgagatatt 480

catactggag ctgaccctga gatcatggat gcacacggag gagaacaagc gccgtacctt 540

gcagaagaat gacatagcag ctgccatcac caggacggat atgtacgatt tcttggtaga 600

tatagttccc agggatgact tgaaggagga gggagttggg ctccctaggg ctggattgcc 660

gcccttgggt gtccctgctg actcatatcc gtatggctac tatgtgccac agcagcaggt 720

cccaggtgca ggaatagcgt atggtggtca gcaaggtcat ccggggtatc tgtggcagga 780

tcctcaggaa cagcaggaag agcctcctgc agagcagcaa agtgattaag aagagtaaat 840

gatccctgtg aattgtcaag aagcttacca cctgattcag aattttactt ttagccaggt 900

tgtcgtctat tctgaattta tgaataggat taggattctc tcatggtagt tgcatttctg 960

ctgtagtgga aaaggattta tgacatgaga gtatgagact aatgggtttc agttactata 1020

ccgtttcctg tcaatccaaa agttggcctt tgcgaggcca ttgatataaa aaaaaaaaaa 1080

aaaaa 1085



106
250
PRT
Oryza sativa

106

Met Glu Pro Ser Ser Gln Pro Gln Pro Ala Ile Gly Val Val Ala Gly
1 5 10 15

Gly Ser Gln Val Tyr Pro Ala Tyr Arg Pro Ala Ala Thr Val Pro Thr
20 25 30

Ala Pro Ala Val Ile Pro Ala Gly Ser Gln Pro Ala Pro Ser Phe Pro
35 40 45

Ala Asn Pro Asp Gln Leu Ser Ala Gln His Gln Leu Val Tyr Gln Gln
50 55 60

Ala Gln Gln Phe His Gln Gln Leu Gln Gln Gln Gln Gln Arg Gln Leu
65 70 75 80

Gln Gln Phe Trp Ala Glu Arg Leu Val Asp Ile Glu Gln Thr Thr Asp
85 90 95

Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys
100 105 110

Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Ile Phe
115 120 125

Ala Lys Ala Cys Glu Ile Phe Ile Leu Glu Leu Thr Leu Arg Ser Trp
130 135 140

Met His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile
145 150 155 160

Ala Ala Ala Ile Thr Arg Thr Asp Met Tyr Asp Phe Leu Val Asp Ile
165 170 175

Val Pro Arg Asp Asp Leu Lys Glu Glu Gly Val Gly Leu Pro Arg Ala
180 185 190

Gly Leu Pro Pro Leu Gly Val Pro Ala Asp Ser Tyr Pro Tyr Gly Tyr
195 200 205

Tyr Val Pro Gln Gln Gln Val Pro Gly Ala Gly Ile Ala Tyr Gly Gly
210 215 220

Gln Gln Gly His Pro Gly Tyr Leu Trp Gln Asp Pro Gln Glu Gln Gln
225 230 235 240

Glu Glu Pro Pro Ala Glu Gln Gln Ser Asp
245 250



107
893
DNA
Oryza sativa

107

gcacgagaaa gagagagctt ttccatcccc aaatcccctc ctcctcctca aaccctagct 60

aagctccgct cgcagcagcc atggatccca ccaaatccag cacgccgccg ccggtgatgg 120

gcgcgcccgt cggcttcccg cctggcgcgt accctccgcc tccccccggc ggcgcagcag 180

cagctgcaga tgttctgggc ggagcagtac cgcgagatcg aggccaccac cgacttcaag 240

aaccacaacc tccccctggc ccgcatcaag aagatcatga aggccgacga ggacgtccgc 300

atgatcgccg ccgaggcccc cgtcgtgttc gcccgcgcct gcgagatgtt catcctcgag 360

ctcacccacc gcggctgggc gcacgccgag gagaacaagc gccgtacgct gcagaagtcc 420

gacattgccg ccgccatcgc gcgcaccgag gtgttcgact tcctcgtcga catcgtgccc 480

cgcgacgacg ccaaggacgc cgacgccgcc gcggccgcgg cggcggccgg catcccccgc 540

cccgccgccg gtgtgccggc caccgatccg ctcgcctact actatgtgcc ccagcagtaa 600

tgtatctgat taaccccttt caagcctttt ctaagcgaag gatgtgttgt tgtttgttgt 660

tgctgttgct gttcttgttg ttgttgttgc cgcgtaataa gatatgttga taatttatgg 720

cttcccctga gcttaaagaa tttgagcttt tggttctaga atctgggtaa aattgttgta 780

atggggaaga ctgtatgact gtatttgtag tgcatgtctt aacttgtcgg atagtgtaat 840

ccgataatta ttatgcggtt agctggttac ctctcaaaaa aaaaaaaaaa aaa 893



108
172
PRT
Oryza sativa

108

Met Asp Pro Thr Lys Ser Ser Thr Pro Pro Pro Val Met Gly Ala Pro
1 5 10 15

Val Gly Phe Pro Pro Gly Ala Tyr Pro Pro Pro Pro Pro Ala Ala Gln
20 25 30

Gln Gln Leu Gln Met Phe Trp Ala Glu Gln Tyr Arg Glu Ile Glu Ala
35 40 45

Thr Thr Asp Phe Lys Asn His Asn Leu Pro Leu Ala Arg Ile Lys Lys
50 55 60

Ile Met Lys Ala Asp Glu Asp Val Arg Met Ile Ala Ala Glu Ala Pro
65 70 75 80

Val Val Phe Ala Arg Ala Cys Glu Met Phe Ile Leu Glu Leu Thr His
85 90 95

Arg Gly Trp Ala His Ala Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys
100 105 110

Ser Asp Ile Ala Ala Ala Ile Ala Arg Thr Glu Val Phe Asp Phe Leu
115 120 125

Val Asp Ile Val Pro Arg Asp Asp Ala Lys Asp Ala Asp Ala Ala Ala
130 135 140

Ala Ala Ala Ala Ala Gly Ile Pro Arg Pro Ala Ala Gly Val Pro Ala
145 150 155 160

Thr Asp Pro Leu Ala Tyr Tyr Tyr Val Pro Gln Gln
165 170



109
1054
DNA
Glycine max

109

gcacgagggg tctctctgtc tctctcggat catcaaaatc agaaagaatt gggggaatgg 60

agaacaacca gcaacaaggc gctcaagccc aatcgggacc gtaccccggc ggcgccggtg 120

gaagtgcagg tgcaggtgca ggtgcaggcg cggccccgtt ccagcacctg ctccagcagc 180

agcagcagca gctgcagatg ttctggtcgt accagcggca agagatcgag cacgtgaacg 240

acttcaagaa ccaccagctc cccttggccc gcatcaagaa gatcatgaag gccgacgagg 300

acgtccgcat gatctccgcc gaggccccca tcctcttcgc caaggcctgc gagctcttca 360

tcctcgagct caccatccgc tcctggctcc acgccgacga gaacaagcgc cgcaccctcc 420

agaagaacga catcgccgcc gccatcactc gcaccgacat tttcgacttc ctcgtcgaca 480

tcgtcccccg cgacgagatc aaggacgacg ccgcgctcgt cggggcaacg gccagtgggg 540

tgccttacta ctacccgccc attggccagc ctgccgggat gatgattggc cgccccgccg 600

tcgatcccgc caccggagtt tatgtccagc cgccctccca ggcctggcag tccgtctggc 660

agtccgccgc cgaggacacg ccctacggca ccggtgccca ggggaacctt gatggccaga 720

gctgagcgac aaccatgccg aaacggactg tcaggagtta tgaagattct gaacttgctt 780

ggaattttga ttgcttgcaa tttggaaatg gttttgttaa ctaaattttt atgggatgac 840

actatgaacc tgttaactcg atgaacagca tgatttaact acttctgtac aaaaatttaa 900

aactaaacaa tgatccttct gtgtgaactt gtttgatcat ctgctaatac tatttatttc 960

ctcgtaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1020

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaa 1054



110
222
PRT
Glycine max

110

Met Glu Asn Asn Gln Gln Gln Gly Ala Gln Ala Gln Ser Gly Pro Tyr
1 5 10 15

Pro Gly Gly Ala Gly Gly Ser Ala Gly Ala Gly Ala Gly Ala Gly Ala
20 25 30

Ala Pro Phe Gln His Leu Leu Gln Gln Gln Gln Gln Gln Leu Gln Met
35 40 45

Phe Trp Ser Tyr Gln Arg Gln Glu Ile Glu His Val Asn Asp Phe Lys
50 55 60

Asn His Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp
65 70 75 80

Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Ile Leu Phe Ala Lys
85 90 95

Ala Cys Glu Leu Phe Ile Leu Glu Leu Thr Ile Arg Ser Trp Leu His
100 105 110

Ala Asp Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala
115 120 125

Ala Ile Thr Arg Thr Asp Ile Phe Asp Phe Leu Val Asp Ile Val Pro
130 135 140

Arg Asp Glu Ile Lys Asp Asp Ala Ala Leu Val Gly Ala Thr Ala Ser
145 150 155 160

Gly Val Pro Tyr Tyr Tyr Pro Pro Ile Gly Gln Pro Ala Gly Met Met
165 170 175

Ile Gly Arg Pro Ala Val Asp Pro Ala Thr Gly Val Tyr Val Gln Pro
180 185 190

Pro Ser Gln Ala Trp Gln Ser Val Trp Gln Ser Ala Ala Glu Asp Thr
195 200 205

Pro Tyr Gly Thr Gly Ala Gln Gly Asn Leu Asp Gly Gln Ser
210 215 220



111
1036
DNA
Glycine max

111

gcacgagccc acacacactc tttctctctc tctctttccc tgatcatcaa aatcagaaaa 60

aattggggga atggagacca acaaccagca acaacaacaa caaggagctc aagcccaatc 120

gggaccctac cccgtcgccg gcgccggcgg cagtgcaggt gcaggtgcag gcgctcctcc 180

ccctttccag caccttctcc agcagcagca gcagcagctc cagatgttct ggtcttacca 240

gcgtcaagaa atcgagcacg tgaacgactt taagaatcac cagctccctc ttgcccgcat 300

caagaagatc atgaaggccg acgaggatgt ccgcatgatc tccgccgagg cccccatcct 360

cttcgccaag gcctgcgagc tcttcatcct cgagctcacc atccgctcct ggctccacgc 420

cgaggagaac aagcgccgca ccctccagaa gaacgacatc gccgccgcca tcacccgcac 480

cgacattttc gacttcctcg ttgatattgt cccccgcgac gagatcaagg acgacgctgc 540

tcttgtgggg gccaccgcca gtggggtgcc ttactactac ccgcccattg gacagcctgc 600

cgggatgatg attggccgcc ccgccgtcga tcccgccacc ggggtttatg tccagccgcc 660

ctcccaggca tggcagtccg tctggcagtc cgctgccgag gacgcttcct atggcaccgg 720

cggggccggt gcccagcgga gccttgatgg ccagagttga gtgacatcga tgccgatgat 780

ggacagtcag gagttatgaa gattctgaac ttgctgcaat ttagaaatgg ttttgtttac 840

taaattttta tgggatgaca ctgtgaacct gttaactcga tgaacagcat gatttaacta 900

cttttgtaca aaaatttaaa actaaacact gatccttctg tgtgaaacat gtatgatcat 960

ctgccaatac tgtttatttc ctcataagtc atgataccac tcgtatactt tgctaaaaaa 1020

aaaaaaaaaa aaaaaa 1036



112
229
PRT
Glycine max

112

Met Glu Thr Asn Asn Gln Gln Gln Gln Gln Gln Gly Ala Gln Ala Gln
1 5 10 15

Ser Gly Pro Tyr Pro Val Ala Gly Ala Gly Gly Ser Ala Gly Ala Gly
20 25 30

Ala Gly Ala Pro Pro Pro Phe Gln His Leu Leu Gln Gln Gln Gln Gln
35 40 45

Gln Leu Gln Met Phe Trp Ser Tyr Gln Arg Gln Glu Ile Glu His Val
50 55 60

Asn Asp Phe Lys Asn His Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile
65 70 75 80

Met Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Ile
85 90 95

Leu Phe Ala Lys Ala Cys Glu Leu Phe Ile Leu Glu Leu Thr Ile Arg
100 105 110

Ser Trp Leu His Ala Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn
115 120 125

Asp Ile Ala Ala Ala Ile Thr Arg Thr Asp Ile Phe Asp Phe Leu Val
130 135 140

Asp Ile Val Pro Arg Asp Glu Ile Lys Asp Asp Ala Ala Leu Val Gly
145 150 155 160

Ala Thr Ala Ser Gly Val Pro Tyr Tyr Tyr Pro Pro Ile Gly Gln Pro
165 170 175

Ala Gly Met Met Ile Gly Arg Pro Ala Val Asp Pro Ala Thr Gly Val
180 185 190

Tyr Val Gln Pro Pro Ser Gln Ala Trp Gln Ser Val Trp Gln Ser Ala
195 200 205

Ala Glu Asp Ala Ser Tyr Gly Thr Gly Gly Ala Gly Ala Gln Arg Ser
210 215 220

Leu Asp Gly Gln Ser
225



113
514
DNA
Glycine max

unsure
(424)
n = A, C, G, or T


113

tagggttttc tcctccccca ttgacccacc gtccatcgca aaggaagtcg cgcccaattt 60

ccatggtttg tagattaaat cttaaagcag taagtcatca tggataaatc agagcagact 120

cagcagcaac atcagcatgg gatgggcgtt gccacaggtg ctagccaaat ggcctattct 180

tctcactacc cgactgctcc catggtggct tctggcacgc ctgctgtagc tgttccttcc 240

ccaactcagg ctccagctgc cttctctagt tctgctcacc agcttgcata ccagcaagca 300

cagcatttcc accaccaaca gcagcaacac caacaacagc agcttcaaat gttctggtca 360

aaccaaatgc aagaaattga gcaaacaatt gactttaaaa accacagtct tcctcttgct 420

cggntaaaan agataatgaa agctgatgaa gatgtccgga tganttctgc aagaagctcc 480

aagtcaatat ttgcaaaagc atgtgnaatg gtca 514



114
126
PRT
Glycine max

UNSURE
(109)
Xaa = any amino acid


114

Met Asp Lys Ser Glu Gln Thr Gln Gln Gln His Gln His Gly Met Gly
1 5 10 15

Val Ala Thr Gly Ala Ser Gln Met Ala Tyr Ser Ser His Tyr Pro Thr
20 25 30

Ala Pro Met Val Ala Ser Gly Thr Pro Ala Val Ala Val Pro Ser Pro
35 40 45

Thr Gln Ala Pro Ala Ala Phe Ser Ser Ser Ala His Gln Leu Ala Tyr
50 55 60

Gln Gln Ala Gln His Phe His His Gln Gln Gln Gln His Gln Gln Gln
65 70 75 80

Gln Leu Gln Met Phe Trp Ser Asn Gln Met Gln Glu Ile Glu Gln Thr
85 90 95

Ile Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Xaa Lys Xaa Ile
100 105 110

Met Lys Ala Asp Glu Asp Val Arg Met Xaa Ser Ala Arg Ser
115 120 125



115
1363
DNA
Glycine max

115

ttcggcacga gttgaaacca aaccaaacca aaccaaacca aacctctctt tctcagtttc 60

tctctcttag ggttttctcc tcccccattg acccaccgtc catcgcaaag gaagtcgcgc 120

ccaatttcca tggaactgta aagagattat agtttgtaga ttaaatctta aagcagtaag 180

tcatcatgga taaatcagag cagactcagc agcaacatca gcatgggatg ggcgttgcca 240

caggtgctag ccaaatggcc tattcttctc actacccgac tgctcccatg gtggcttctg 300

gcacgcctgc tgtagctgtt ccttccccaa ctcaggctcc agctgccttc tctagttctg 360

ctcaccagct tgcataccag caagcacagc atttccacca ccaacagcag caacaccaac 420

aacagcagct tcaaatgttc tggtcaaacc aaatgcaaga aattgagcaa acaattgact 480

ttaaaaacca cagtcttcct cttgctcgga taaaaaagat aatgaaagct gatgaagatg 540

tccggatgat ttctgcagaa gctccagtca tatttgcaaa agcatgtgaa atgttcatat 600

tagagttgac gttgagatct tggatccaca cagaagagaa caagaggaga actctacaaa 660

agaatgatat agcagctgct atttcgagaa acgatgtttt tgatttcttg gttgatatta 720

tcccaagaga tgagttgaaa gaggaaggac ttggaataac caaggctact attccattgg 780

tgaattctcc agctgatatg ccatattact atgtccctcc acagcatcct gttgtaggac 840

ctcctgggat gatcatgggc aagcccgttg gtgctgagca agcaacgctg tattctacac 900

agcagcctcg acctcccatg gcgttcatgc catggcccca tacacaaccc cagcaacagc 960

agccacccca acatcaacaa acagactcat gatgaccatg caattcaatt aggtcggaaa 1020

gtagcatgca ccttatgatt attacaaatt tacttaatgc ctttaagtca gctgtagttt 1080

agtgttttgc attgaaaaat gccaaagatt gtttgaggtt tcttgcactc atttatgatt 1140

gtatgagctc ttatgctgag ttacttttgg ttgtgtttat ttgaggtact ggtgtggtag 1200

ttaaattagt ttgtagctgt ccataagtaa acagcgtagc tgcttaatta ggaggtctga 1260

aatgatgaaa tagtttgtat tgttattgca gaaggtaggt tttattcagt atttcattct 1320

attgcaatgg ctgaatttaa tgctcaaaaa aaaaaaaaaa aaa 1363



116
268
PRT
Glycine max

116

Met Asp Lys Ser Glu Gln Thr Gln Gln Gln His Gln His Gly Met Gly
1 5 10 15

Val Ala Thr Gly Ala Ser Gln Met Ala Tyr Ser Ser His Tyr Pro Thr
20 25 30

Ala Pro Met Val Ala Ser Gly Thr Pro Ala Val Ala Val Pro Ser Pro
35 40 45

Thr Gln Ala Pro Ala Ala Phe Ser Ser Ser Ala His Gln Leu Ala Tyr
50 55 60

Gln Gln Ala Gln His Phe His His Gln Gln Gln Gln His Gln Gln Gln
65 70 75 80

Gln Leu Gln Met Phe Trp Ser Asn Gln Met Gln Glu Ile Glu Gln Thr
85 90 95

Ile Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile
100 105 110

Met Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val
115 120 125

Ile Phe Ala Lys Ala Cys Glu Met Phe Ile Leu Glu Leu Thr Leu Arg
130 135 140

Ser Trp Ile His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn
145 150 155 160

Asp Ile Ala Ala Ala Ile Ser Arg Asn Asp Val Phe Asp Phe Leu Val
165 170 175

Asp Ile Ile Pro Arg Asp Glu Leu Lys Glu Glu Gly Leu Gly Ile Thr
180 185 190

Lys Ala Thr Ile Pro Leu Val Asn Ser Pro Ala Asp Met Pro Tyr Tyr
195 200 205

Tyr Val Pro Pro Gln His Pro Val Val Gly Pro Pro Gly Met Ile Met
210 215 220

Gly Lys Pro Val Gly Ala Glu Gln Ala Thr Leu Tyr Ser Thr Gln Gln
225 230 235 240

Pro Arg Pro Pro Met Ala Phe Met Pro Trp Pro His Thr Gln Pro Gln
245 250 255

Gln Gln Gln Pro Pro Gln His Gln Gln Thr Asp Ser
260 265



117
1505
DNA
Glycine max

117

gcacgagctc caccgtccat tgcaaagtct tgcgcccaat ttccatggaa ctgtaaagag 60

aggatagtta gaagattaaa tcttaaagca gtaagtcatc atggataaat cagagcagac 120

tcaacagcag cagcagcaac aacagcatgt gatgggagtt gccgcagggg ctagccaaat 180

ggcctattct tctcactacc cgactgcttc catggtggct tctggcacgc ccgctgtaac 240

tgctccttcc ccaactcagg ctccagctgc cttctctagt tctgctcacc agcttgcata 300

ccagcaagca cagcatttcc accaccaaca gcagcaacac caacaacagc agcttcaaat 360

gttctggtca aaccaaatgc aagaaattga gcaaacaatt gactttaaaa accatagcct 420

tcctcttgct cggataaaaa agataatgaa agctgatgaa gatgtccgga tgatttcagc 480

agaagctccg gtcatatttg caaaagcttg tgaaatgttc atattagagt tgacgttgcg 540

atcttggatc cacacagaag agaacaagag gagaactcta caaaagaatg atatagcagc 600

tgctatttcg agaaacgatg tttttgattt cttggttgat attattccaa gagatgagtt 660

gaaagaggaa ggacttggaa taaccaaggc tactattccg ttagtgggtt ctccagctga 720

tatgccatat tactatgtcc ctccacagca tcctgttgta ggaccacctg ggatgatcat 780

gggcaagccc attggcgctg agcaagcaac actatattct acacagcagc ctcgacctcc 840

tgtggcgttc atgccatggc ctcatacaca acccctgcaa cagcagccac cccaacatca 900

acaaacagac tcatgatgac tatgcaattc aattaggttg gaaagtagcc tgcacctttt 960

gattattaca aatttactta atgcctttca gccagctgta gtttagtgtt gtgcattgaa 1020

aaaaagcaaa agattgtttt gaggtttctt gcactcattt atgattgtat gagctcttgt 1080

gatgagttac ttttggttgt gtttactatt ggtgtagtgg ttaaattatt tggcagctgt 1140

ccataaccag agagcgtagc tgcttaatta ggaggtttga tatgatgaaa tagtttgtat 1200

tgttattgca gaaggtaggt ttaattcagt attccattct actgcaatgg ctgaatttat 1260

tgctcatctg catagtacta gttgatgttt tttcctgtga ctcgttatgt gttagagtgc 1320

gaagaagaat gagtgtgcca tatttattct tcccctgttc ttgcgccaca ctctcggaaa 1380

aacaaatgtt tccgatcatt tcaattattt ccaggaacat caatatagtg gttgatgttt 1440

aatgctgtca ctgcaaaaaa aaatatgttt tttacagttg gaaaaaaaaa aaaaaaaaaa 1500

aaaaa 1505



118
271
PRT
Glycine max

118

Met Asp Lys Ser Glu Gln Thr Gln Gln Gln Gln Gln Gln Gln Gln His
1 5 10 15

Val Met Gly Val Ala Ala Gly Ala Ser Gln Met Ala Tyr Ser Ser His
20 25 30

Tyr Pro Thr Ala Ser Met Val Ala Ser Gly Thr Pro Ala Val Thr Ala
35 40 45

Pro Ser Pro Thr Gln Ala Pro Ala Ala Phe Ser Ser Ser Ala His Gln
50 55 60

Leu Ala Tyr Gln Gln Ala Gln His Phe His His Gln Gln Gln Gln His
65 70 75 80

Gln Gln Gln Gln Leu Gln Met Phe Trp Ser Asn Gln Met Gln Glu Ile
85 90 95

Glu Gln Thr Ile Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile
100 105 110

Lys Lys Ile Met Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu
115 120 125

Ala Pro Val Ile Phe Ala Lys Ala Cys Glu Met Phe Ile Leu Glu Leu
130 135 140

Thr Leu Arg Ser Trp Ile His Thr Glu Glu Asn Lys Arg Arg Thr Leu
145 150 155 160

Gln Lys Asn Asp Ile Ala Ala Ala Ile Ser Arg Asn Asp Val Phe Asp
165 170 175

Phe Leu Val Asp Ile Ile Pro Arg Asp Glu Leu Lys Glu Glu Gly Leu
180 185 190

Gly Ile Thr Lys Ala Thr Ile Pro Leu Val Gly Ser Pro Ala Asp Met
195 200 205

Pro Tyr Tyr Tyr Val Pro Pro Gln His Pro Val Val Gly Pro Pro Gly
210 215 220

Met Ile Met Gly Lys Pro Ile Gly Ala Glu Gln Ala Thr Leu Tyr Ser
225 230 235 240

Thr Gln Gln Pro Arg Pro Pro Val Ala Phe Met Pro Trp Pro His Thr
245 250 255

Gln Pro Leu Gln Gln Gln Pro Pro Gln His Gln Gln Thr Asp Ser
260 265 270



119
730
DNA
Glycine max

119

gcacgagtga ctttaaaaac catagccttc ctcttgctcg gataaaaaag ataatgaaag 60

ctgatgaaga tgtccggatg atttcagcag aagctccggt catatttgca aaagcttgtg 120

aaatgttcat attagagttg acgttgcgat cttggatcca cacagaagag aacaagagga 180

gaactctaca aaagaatgat atagcagctg ctatttcgag aaacgatgtt tttgatttct 240

tggttgatat tattccaaga gatgagttga aagaggaagg acttggaata accaaggcta 300

ctattccgtt agtgggttct ccagctgata tgccatatta ctatgtccct ccacagcatc 360

ctgttgtagg accacctggg atgatcatgg gcaagcccat tggcgctgag caagcaacac 420

tatattctac acagcagcct cgacctcctg tggcgttcat gccatggcct catacacaac 480

ccctgcaaca gcagccaccc caacatcaac aaacagactc atgatgacta tgcaattcaa 540

ttaggttgga aagtagcctg caccttttga ttattacaaa tttacttaat gcctttcagc 600

cagctgtagt ttagtgttgt gcattgaaaa aaagcaaaag attgttttga ggtttcttgc 660

actcatttat gattgtatga gctcttgtga tgagttactt ttggttgtgt ttaaaaaaaa 720

aaaaaaaaaa 730



120
171
PRT
Glycine max

120

Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile Met
1 5 10 15

Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Ile
20 25 30

Phe Ala Lys Ala Cys Glu Met Phe Ile Leu Glu Leu Thr Leu Arg Ser
35 40 45

Trp Ile His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp
50 55 60

Ile Ala Ala Ala Ile Ser Arg Asn Asp Val Phe Asp Phe Leu Val Asp
65 70 75 80

Ile Ile Pro Arg Asp Glu Leu Lys Glu Glu Gly Leu Gly Ile Thr Lys
85 90 95

Ala Thr Ile Pro Leu Val Gly Ser Pro Ala Asp Met Pro Tyr Tyr Tyr
100 105 110

Val Pro Pro Gln His Pro Val Val Gly Pro Pro Gly Met Ile Met Gly
115 120 125

Lys Pro Ile Gly Ala Glu Gln Ala Thr Leu Tyr Ser Thr Gln Gln Pro
130 135 140

Arg Pro Pro Val Ala Phe Met Pro Trp Pro His Thr Gln Pro Leu Gln
145 150 155 160

Gln Gln Pro Pro Gln His Gln Gln Thr Asp Ser
165 170



121
1139
DNA
Glycine max

121

gcacgagaca cagcttttgt tctcgcactt cgctgtctga ggttctggat tctcagtgtt 60

tgcgaagcgc tgcatcatcc tttggggaag aatggatcat caagggcata gccagaaccc 120

atctatgggg gtggttggta gtggagctca attagcatat ggttctaacc catatcagcc 180

aggccaaata actgggccac cggggtctgt tgtgacatca gttggtacca ttcaatccac 240

acctgctgga gctcagctag gacagcatca acttgcttat cagcatattc atcagcaaca 300

acaacaccag cttcagcaac agctccaaca attttggtca aaccagtacc aagaaattga 360

gaaggttact gatttcaaga accacagtct tcccctggca aggatcaaga agattatgaa 420

ggctgacgag gatgttagga tgatatcagc cgaagcacca gtcatctttg caagggcatg 480

tgaaatgttc atattagagt taaccctgcg ttcttggaat cacactgaag agaacaaaag 540

gcgaacactt caaaaaaatg atattgctgc tgcaatcaca aggactgaca tctttgattt 600

cttggttgac attgtgcctc gtgaggactt gaaagatgaa gtgcttgcat caatcccaag 660

aggaacaatg cctgttgcag ggcctgctga tgcccttcca tattgctaca tgccgcctca 720

gcatgcgtcc caagttggag ctgctggtgt tataatgggt aagcctgtga tggacccaaa 780

catgtatgct cagcagtctc acccctacat ggcaccacaa atgtggccac agccaccaga 840

ccaacgacag tcgtccccag aacattagct gatgtgtcgt ggaaattaag ataaccaggc 900

accggaatca gttgtgaatg tcaaactgaa tggttgggaa gatccatact acattgcgag 960

cagaagctgt agctgatagt ttacatgcaa tgcagactat aaacatatgt agataatgtg 1020

ctagggaaaa cttaacctta tctttgattt agctggataa aatggtattt ttcatgttta 1080

aatttacagg tcatcagatg ataatattta tttactggtg caaaaaaaaa aaaaaaaaa 1139



122
258
PRT
Glycine max

122

Met Asp His Gln Gly His Ser Gln Asn Pro Ser Met Gly Val Val Gly
1 5 10 15

Ser Gly Ala Gln Leu Ala Tyr Gly Ser Asn Pro Tyr Gln Pro Gly Gln
20 25 30

Ile Thr Gly Pro Pro Gly Ser Val Val Thr Ser Val Gly Thr Ile Gln
35 40 45

Ser Thr Pro Ala Gly Ala Gln Leu Gly Gln His Gln Leu Ala Tyr Gln
50 55 60

His Ile His Gln Gln Gln Gln His Gln Leu Gln Gln Gln Leu Gln Gln
65 70 75 80

Phe Trp Ser Asn Gln Tyr Gln Glu Ile Glu Lys Val Thr Asp Phe Lys
85 90 95

Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp
100 105 110

Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Ile Phe Ala Arg
115 120 125

Ala Cys Glu Met Phe Ile Leu Glu Leu Thr Leu Arg Ser Trp Asn His
130 135 140

Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala
145 150 155 160

Ala Ile Thr Arg Thr Asp Ile Phe Asp Phe Leu Val Asp Ile Val Pro
165 170 175

Arg Glu Asp Leu Lys Asp Glu Val Leu Ala Ser Ile Pro Arg Gly Thr
180 185 190

Met Pro Val Ala Gly Pro Ala Asp Ala Leu Pro Tyr Cys Tyr Met Pro
195 200 205

Pro Gln His Ala Ser Gln Val Gly Ala Ala Gly Val Ile Met Gly Lys
210 215 220

Pro Val Met Asp Pro Asn Met Tyr Ala Gln Gln Ser His Pro Tyr Met
225 230 235 240

Ala Pro Gln Met Trp Pro Gln Pro Pro Asp Gln Arg Gln Ser Ser Pro
245 250 255

Glu His



123
1493
DNA
Triticum aestivum

123

ggcaccagct ctggcttcca agtctataca taatataggg accgagcttg cggttttgcc 60

aagggtgatg gggaccgagc aagggaagga aggaacggga gcgggggagg ggcgcgtgga 120

ggtgcgcacg gggccgaggc cagcgctgcc ggcgccgcag cagcgggcgg tggacgggtt 180

ctggagggag cggcaggagg agatggaggc gacggcggac ttcaacgacc gcatactgcc 240

catggcccgc ctcaagaggc tcatccgcgc cgaggaggac ggcatgatga tcgccgccga 300

cacgccggcg tacctggcca agctctgcga gctcttcgtg caggagctcg ccgtgcgcgc 360

ctgggcgtgc gcccaatccc accaccgccg catcatactg gaatcggaca tcgccgaggc 420

catcgccttc acccagtcgt acgacttcct cgccaccgtg ctcctcgagc accaacggga 480

ggcgcggctg gccggccgtg ctgctatccc gacaacggtt ccggtgacgg cggcgagggc 540

aaggctcatc accaggaagc gccacatgcc ggacccgaat cctccacggc cggtgcatgg 600

ggtgcggaga attcgtcctc gtgcgcttcc tatcccgccg ccgtcggact ttcgctacgt 660

gccggttcca tttccgttca cctcggcgcc gataggagcc gcagcgatgg cggaggggct 720

gatgattctc ccacccatca accacgcgac taccgagcgc gtgttcttcc tggacaggaa 780

cagcggcact gacttcgcag gtgaaaactc tgctgctgaa actatagcat ctccgcctcc 840

tccggcaggg cctgcaggag cagtggcgct gcccactgtc catcctgctg cttactactt 900

gtgcgcttac ccggtgacca acgacgttga ggcctttgcc gttggcaaca ctgatcctga 960

tgtcatccca ccggagattg tagtgggaga cgtcgccatc ccaccggaga ttatagaggg 1020

aaacgtcgcc gatggcaacg gcgacggcgg acagcagcag cagcagagcg aaaaccttgg 1080

tggtaatggt gagagtgtgg tggtgtcgca aagcaatggt gtgcaggaag atggtgcaga 1140

tgggatgttt ctgaaggaga tcctcatgga tgaagacctg atgtttcccg acgctgagct 1200

ttttccgttg gtgggcgctg cacctggtcc agaggatttc atcgtcgacc aagatgttct 1260

cgacgacgtc ttcgccaacc cgagcagcag cgcaagcagc gactgaaccg aaagaagatc 1320

agagcgggac gcagcatcgg ttgattcatc tatcgtctct cgacctgcta ctctatgcta 1380

gccgctatat cggttaataa atttgggaat aagtttgtgt tcgtgcgtgt gacatggact 1440

gtatggttcg ccctgaattt atcgtattgc aatatatagc cgtgattgtg tgt 1493



124
434
PRT
Triticum aestivum

124

Ala Pro Ala Leu Ala Ser Lys Ser Ile His Asn Ile Gly Thr Glu Leu
1 5 10 15

Ala Val Leu Pro Arg Val Met Gly Thr Glu Gln Gly Lys Glu Gly Thr
20 25 30

Gly Ala Gly Glu Gly Arg Val Glu Val Arg Thr Gly Pro Arg Pro Ala
35 40 45

Leu Pro Ala Pro Gln Gln Arg Ala Val Asp Gly Phe Trp Arg Glu Arg
50 55 60

Gln Glu Glu Met Glu Ala Thr Ala Asp Phe Asn Asp Arg Ile Leu Pro
65 70 75 80

Met Ala Arg Leu Lys Arg Leu Ile Arg Ala Glu Glu Asp Gly Met Met
85 90 95

Ile Ala Ala Asp Thr Pro Ala Tyr Leu Ala Lys Leu Cys Glu Leu Phe
100 105 110

Val Gln Glu Leu Ala Val Arg Ala Trp Ala Cys Ala Gln Ser His His
115 120 125

Arg Arg Ile Ile Leu Glu Ser Asp Ile Ala Glu Ala Ile Ala Phe Thr
130 135 140

Gln Ser Tyr Asp Phe Leu Ala Thr Val Leu Leu Glu His Gln Arg Glu
145 150 155 160

Ala Arg Leu Ala Gly Arg Ala Ala Ile Pro Thr Thr Val Pro Val Thr
165 170 175

Ala Ala Arg Ala Arg Leu Ile Thr Arg Lys Arg His Met Pro Asp Pro
180 185 190

Asn Pro Pro Arg Pro Val His Gly Val Arg Arg Ile Arg Pro Arg Ala
195 200 205

Leu Pro Ile Pro Pro Pro Ser Asp Phe Arg Tyr Val Pro Val Pro Phe
210 215 220

Pro Phe Thr Ser Ala Pro Ile Gly Ala Ala Ala Met Ala Glu Gly Leu
225 230 235 240

Met Ile Leu Pro Pro Ile Asn His Ala Thr Thr Glu Arg Val Phe Phe
245 250 255

Leu Asp Arg Asn Ser Gly Thr Asp Phe Ala Gly Glu Asn Ser Ala Ala
260 265 270

Glu Thr Ile Ala Ser Pro Pro Pro Pro Ala Gly Pro Ala Gly Ala Val
275 280 285

Ala Leu Pro Thr Val His Pro Ala Ala Tyr Tyr Leu Cys Ala Tyr Pro
290 295 300

Val Thr Asn Asp Val Glu Ala Phe Ala Val Gly Asn Thr Asp Pro Asp
305 310 315 320

Val Ile Pro Pro Glu Ile Val Val Gly Asp Val Ala Ile Pro Pro Glu
325 330 335

Ile Ile Glu Gly Asn Val Ala Asp Gly Asn Gly Asp Gly Gly Gln Gln
340 345 350

Gln Gln Gln Ser Glu Asn Leu Gly Gly Asn Gly Glu Ser Val Val Val
355 360 365

Ser Gln Ser Asn Gly Val Gln Glu Asp Gly Ala Asp Gly Met Phe Leu
370 375 380

Lys Glu Ile Leu Met Asp Glu Asp Leu Met Phe Pro Asp Ala Glu Leu
385 390 395 400

Phe Pro Leu Val Gly Ala Ala Pro Gly Pro Glu Asp Phe Ile Val Asp
405 410 415

Gln Asp Val Leu Asp Asp Val Phe Ala Asn Pro Ser Ser Ser Ala Ser
420 425 430

Ser Asp



125
660
DNA
Triticum aestivum

unsure
(483)
n = A, C, G, or T


125

ggcaccgagc tagcttggca atggccgcga gggcgtgtcc tgctgcttct ggttaccgtg 60

tgtgctgaag catctgacgc gcttgcgccg agcagcagga gctagccgtt catgctcttc 120

ttccctcccc ttggcatctg aagcagtaag agctcaagtt cacagagggc gttcgtccga 180

tctacaaagc ccagctgtac atcgccttag ctagcttgca gatcgcaagc tagatagtaa 240

tggagaacca ccagctgccc tacaccaccc agccgccggc aacgggcgcg gccggaggag 300

ccccggtgcc tggcgtgcct gggccaccgc cggtgccaca ccaccacctg ctccagcagc 360

agcaggccca gctgcaggcg ttctgggcgt accagcggca ggaggcggag cgcgcatcgg 420

cgtccgactt caagaaccac cagctgccgc tggctcggat caagaagatc atgaaggccg 480

acnaagacgt gcgcatgatc tccgcggagg cgcccgtgct cttcgccaag gcctgcgagc 540

tctttattct cgaagctcac cattccgctt cctggctgca cgcccgagga agaacaagcc 600

gccgcacaac ttgnagcgca aacgacgttn cccgcttgcc aatnggngcc gccacccgac 660



126
147
PRT
Triticum aestivum

UNSURE
(82)
Xaa = any amino acid


126

Met Glu Asn His Gln Leu Pro Tyr Thr Thr Gln Pro Pro Ala Thr Gly
1 5 10 15

Ala Ala Gly Gly Ala Pro Val Pro Gly Val Pro Gly Pro Pro Pro Val
20 25 30

Pro His His His Leu Leu Gln Gln Gln Gln Ala Gln Leu Gln Ala Phe
35 40 45

Trp Ala Tyr Gln Arg Gln Glu Ala Glu Arg Ala Ser Ala Ser Asp Phe
50 55 60

Lys Asn His Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala
65 70 75 80

Asp Xaa Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Leu Phe Ala
85 90 95

Lys Ala Cys Glu Leu Phe Ile Leu Glu Ala His His Ser Ala Ser Trp
100 105 110

Leu His Ala Arg Gly Arg Thr Ser Arg Arg Thr Thr Xaa Ser Ala Asn
115 120 125

Asp Val Xaa Arg Leu Pro Xaa Gly Ala Ala Thr Arg Arg Xaa Phe Glu
130 135 140

Xaa Phe Leu
145



127
1874
DNA
Triticum aestivum

127

gcacgagccc acccacaacc ctagctcccc cgaacccatg gatcccacca aatccagcac 60

cccgccgccg ccccccgtcc tgggcgcgcc cgtcggctac ccgccggggg cgtaccctcc 120

tccgccgggc gcccccgcgg ccgcctaccc gccgcagctc tacgcgccgc cgggcgccgc 180

cgccgcccag caggccgcgg cgcagcagca gcagcagctg caggtgttct gggcggagca 240

gtaccgcgag atcgaggcca ccaccgactt caagaaccac aacctcccgc tggcccggat 300

caagaagatc atgaaggccg acgaggacgt ccgcatgatc gccgccgagg cccccgtcgt 360

cttcgcccgc gcctgcgaga tgttcatcct cgagctcacc caccgcggct gggcgcacgc 420

cgaggagaac aagcgccgca cgctccagaa gtccgacatt gcggccgcca tcgcccgcac 480

cgaggtcttc gacttcctcg tggacatcgt gccccgggac gacgccaagg acgccgaggc 540

ggccgccgcc gcggccatgg ccacggcggc ggccgggatc ccgcgcccgg ccgccggcgt 600

gcctgccacc gacccgagta tggcatacta ctatgtcccc cagcagtaat gtatcatcga 660

tctaaacttg cgcatttcta atcggagaat gtgttgttgt tctgtgactg tccttggtgc 720

tgttgttgct gcggcgtaat aagatttatg ggcctcccct gagcttatga attgagctgt 780

tcggttctag tattacagta ggattgttgt aatgggggag gccgtatgat tgcttccgta 840

gtgcatgact aactggccac ccagtgtaat ctgataacta ttatctggcg cctcccatgg 900

ttactatgta tttatgttct tcacacagtc ctctttgtct ctaccacttc gaggagttct 960

tcggaaggat gggctccaag atgcttctgg tcaccgctct cttggtgggc atagcctctc 1020

agagctatgc caccaggagc cttgacggaa accacttggc tgatcagaag tacggcggcg 1080

gcggctacgg aggtggcggt gggggctccg gaggtggtgg tggctacgga ggaggtggca 1140

gcggcggcgg gggtggctat ggaggaggcg gcggcggtgg ctacggagga ggaggcggcg 1200

gttacacacc gatgccaaca ccgtcgaccc ccagccacag cggatcctgc gactactgga 1260

agggccaccc ggagaagatc atcgactgca tcggcagcct gggcagcatc ctgggctccc 1320

tcggagaggt gtgccactcc ttcttcggca gcaagatcca taccctgcag gacgcgctgt 1380

gcaacacccg gaccgactgc tacggcgacc tgctgcgcga gggcgccgcc gcctacatca 1440

acgccatcgc cgccaagaag gagaagttcg cctacaccgc ctaccaggtc aaggagtgcg 1500

tcgccgtcgg gctcacctcc gagttcgccg ccgccgcgca ggccgccatg ttgaagaagg 1560

ccaactacgc ctgccactac taggaggcta ggctaccggc cggccgcccc agctggtggt 1620

cgtcggtggc taaataagtc catatatgca tgcacgtgtc gtgcatgttt tcatgcagtt 1680

tcccggatgc gcgcgcgcgt gtcctccgct atgcctttat gtgtttgctt gccgtttgat 1740

gatgcatgcc atgccgtctc atatatacgt agtgatgctt aatgctttgc ttgcttttct 1800

tatcttcgtt ggtgatgtaa gaataatttg attgaggagt tattagtgaa agacatagta 1860

tgcaaaaaaa aaaa 1874



128
203
PRT
Triticum aestivum

128

Met Asp Pro Thr Lys Ser Ser Thr Pro Pro Pro Pro Pro Val Leu Gly
1 5 10 15

Ala Pro Val Gly Tyr Pro Pro Gly Ala Tyr Pro Pro Pro Pro Gly Ala
20 25 30

Pro Ala Ala Ala Tyr Pro Pro Gln Leu Tyr Ala Pro Pro Gly Ala Ala
35 40 45

Ala Ala Gln Gln Ala Ala Ala Gln Gln Gln Gln Gln Leu Gln Val Phe
50 55 60

Trp Ala Glu Gln Tyr Arg Glu Ile Glu Ala Thr Thr Asp Phe Lys Asn
65 70 75 80

His Asn Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu
85 90 95

Asp Val Arg Met Ile Ala Ala Glu Ala Pro Val Val Phe Ala Arg Ala
100 105 110

Cys Glu Met Phe Ile Leu Glu Leu Thr His Arg Gly Trp Ala His Ala
115 120 125

Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Ser Asp Ile Ala Ala Ala
130 135 140

Ile Ala Arg Thr Glu Val Phe Asp Phe Leu Val Asp Ile Val Pro Arg
145 150 155 160

Asp Asp Ala Lys Asp Ala Glu Ala Ala Ala Ala Ala Ala Met Ala Thr
165 170 175

Ala Ala Ala Gly Ile Pro Arg Pro Ala Ala Gly Val Pro Ala Thr Asp
180 185 190

Pro Ser Met Ala Tyr Tyr Tyr Val Pro Gln Gln
195 200



129
629
DNA
Amaranthus retroflexus

unsure
(566)..(567)..(568)
n = A, C, G, or T


129

gcacgaggat ggatcatcat catcgtggag ggttccatgg ttaccgcaaa caacatcccc 60

tttctaagtc ctcctcttct gaaatgagat tgacatcgga ggtgttaccg gctgagatga 120

atcacatacg cccaactagc aatggaaaag gagtatcaca tgacatgaac aaccatacca 180

ataaccatca tccctacaac aatagcaaca acaacaacaa tggtttcagc aacggaaata 240

gtaatcactc agcatcaacc gatcaagata acaatgagtg cactgtacgc gagcaagatc 300

gctttatgcc catcgccaat gtcattagga tcatgcgcaa gattcttcct cctcatgcca 360

aaatctccga tgatgctaag gaaactatcc aggagtgtgt atcagagtac atcagcttca 420

taacaggtga agccaacgag aggtgccaaa gggaacaacg taagaccata actgctgaag 480

atgttctttg ggcgatgagc aagttgggat tcgatgacta catcgaaccc ctcacactgt 540

acttgcatcg atacagggaa ctcgannngg aacgtggttc catccgcact tgtgagccac 600

tcctcnnnct cagtcgtgct gccatnnnn 629



130
198
PRT
Amaranthus retroflexus

UNSURE
(186)..(187)
Xaa = any amino acid


130

Met Asp His His His Arg Gly Gly Phe His Gly Tyr Arg Lys Gln His
1 5 10 15

Pro Leu Ser Lys Ser Ser Ser Ser Glu Met Arg Leu Thr Ser Glu Val
20 25 30

Leu Pro Ala Glu Met Asn His Ile Arg Pro Thr Ser Asn Gly Lys Gly
35 40 45

Val Ser His Asp Met Asn Asn His Thr Asn Asn His His Pro Tyr Asn
50 55 60

Asn Ser Asn Asn Asn Asn Asn Gly Phe Ser Asn Gly Asn Ser Asn His
65 70 75 80

Ser Ala Ser Thr Asp Gln Asp Asn Asn Glu Cys Thr Val Arg Glu Gln
85 90 95

Asp Arg Phe Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg Lys Ile
100 105 110

Leu Pro Pro His Ala Lys Ile Ser Asp Asp Ala Lys Glu Thr Ile Gln
115 120 125

Glu Cys Val Ser Glu Tyr Ile Ser Phe Ile Thr Gly Glu Ala Asn Glu
130 135 140

Arg Cys Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu Asp Val Leu
145 150 155 160

Trp Ala Met Ser Lys Leu Gly Phe Asp Asp Tyr Ile Glu Pro Leu Thr
165 170 175

Leu Tyr Leu His Arg Tyr Arg Glu Leu Xaa Xaa Glu Arg Gly Ser Ile
180 185 190

Arg Thr Cys Glu Pro Leu
195



131
625
DNA
Momordica charantia

unsure
(597)..(598)..(599)
n = A, C, G, or T


131

gcacgaggct agctagctag gtctctctac tcagttagag agagaaagaa aaagaaaaca 60

aggggaagag agagagagag gcatggaata tggaggagga ggaggagatg ggttccatag 120

ctacagaagg cagcagccaa acacaaaacc aagctctgct ttgaacatgt tgctgaccac 180

aaacaagcca tccgccaaca accaccacca ccacttaaac ggccaaaacg ccaccaccac 240

caccaactcc tctgctgctg ccgccccgac cctggccccg gccgctgctg ccaacaacaa 300

cgagcagcag tgcgtcgtgc gggagcaaga ccaatacatg ccgatcgcca acgtgatacg 360

catcatgcgg cggatcttac cctcccatgc aaagatatcc gacgatgcca aggagaccat 420

ccaagagtgt gtgtcggagt acattagctt catcaccggc gaggccaacg agcggtgcca 480

gcgagagcag cgcaagacgg tgacggcgga ggacgtcctt tgggccatgg ggaagcttgg 540

cttcgacgac tacatcgagc cactcaccgt gttcctcaac cgctaccggg agtcagnnng 600

cgatcgaatc cgaacggagn nnntc 625



132
179
PRT
Momordica charantia

UNSURE
(172)..(173)
Xaa = any amino acid


132

Met Glu Tyr Gly Gly Gly Gly Gly Asp Gly Phe His Ser Tyr Arg Arg
1 5 10 15

Gln Gln Pro Asn Thr Lys Pro Ser Ser Ala Leu Asn Met Leu Leu Thr
20 25 30

Thr Asn Lys Pro Ser Ala Asn Asn His His His His Leu Asn Gly Gln
35 40 45

Asn Ala Thr Thr Thr Thr Asn Ser Ser Ala Ala Ala Ala Pro Thr Leu
50 55 60

Ala Pro Ala Ala Ala Ala Asn Asn Asn Glu Gln Gln Cys Val Val Arg
65 70 75 80

Glu Gln Asp Gln Tyr Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg
85 90 95

Arg Ile Leu Pro Ser His Ala Lys Ile Ser Asp Asp Ala Lys Glu Thr
100 105 110

Ile Gln Glu Cys Val Ser Glu Tyr Ile Ser Phe Ile Thr Gly Glu Ala
115 120 125

Asn Glu Arg Cys Gln Arg Glu Gln Arg Lys Thr Val Thr Ala Glu Asp
130 135 140

Val Leu Trp Ala Met Gly Lys Leu Gly Phe Asp Asp Tyr Ile Glu Pro
145 150 155 160

Leu Thr Val Phe Leu Asn Arg Tyr Arg Glu Ser Xaa Xaa Asp Arg Ile
165 170 175

Arg Thr Glu



133
1173
DNA
Zea mays

133

ccacgcgtcc gccaccacac cacgagcgcg cgataaccct agctagcttc aggtagtagc 60

gagagccaat ggactccagc agcttcctcc ctgccgccgg cgcggagaat ggctcggcgg 120

cgggcggcgc caacaatggc ggcgctgctc agcagcatgc ggcgccggcg atccgcgagc 180

aggaccggct gatgccgatc gcgaacgtga tccgcatcat gcggcgcgtg ctgccggcgc 240

acgccaagat ctcggacgac gccaaggaga cgatccagga gtgcgtgtcg gagtacatca 300

gcttcatcac gggggaggcc aacgagcggt gccagcggga gcagcgcaag accatcaccg 360

ccgaggacgt gctgtgggcc atgagccgcc tcggcttcga cgactacgtc gagccgctcg 420

gcgcctacct ccaccgctac cgcgagttcg agggcgacgc gcgcggcgtc gggctcgtcc 480

cgggggccgc cccatcgcgc ggcggcgacc accacccgca ctccatgtcg ccagcggcga 540

tgctcaagtc ccgcgggcca gtctccggag ccgccatgct accgcaccac caccaccacc 600

acgacatgca gatgcacgcc gccatgtacg ggggaacggc cgtgcccccg ccggccgggc 660

ctcctcacca cggcgggttc ctcatgccac acccacaggg tagtagccac tacctgcctt 720

acgcgtacga gcccacgtac ggcggtgagc acgccatggc tgcatactat ggaggcgccg 780

cgtacgcgcc cggcaacggc gggagcggcg acggcagtgg cagtggcggc ggtggcggga 840

gcgcgtcgca cacaccgcag ggcagcggcg gcttggagca cccgcacccg ttcgcgtaca 900

agtagctagt tcgtacgtcg ttcgacttga gcaagccatc gatctgctga tctgaacgta 960

cgctgtattg tacacgcatg cacgtacgta tcggcggcta gctctcctgt ttaagttgta 1020

ctgtgattct gtcccggccg gctagcaact tagtatcttc cttcagtctc tagtttctta 1080

gcagtcgtag aagtgttcaa tgcttgccag tgtgttgttt tagggccggg gtaaaccatc 1140

cgatgagatt atttcaaaaa aaaaaaaaaa aaa 1173



134
278
PRT
Zea mays

134

Met Asp Ser Ser Ser Phe Leu Pro Ala Ala Gly Ala Glu Asn Gly Ser
1 5 10 15

Ala Ala Gly Gly Ala Asn Asn Gly Gly Ala Ala Gln Gln His Ala Ala
20 25 30

Pro Ala Ile Arg Glu Gln Asp Arg Leu Met Pro Ile Ala Asn Val Ile
35 40 45

Arg Ile Met Arg Arg Val Leu Pro Ala His Ala Lys Ile Ser Asp Asp
50 55 60

Ala Lys Glu Thr Ile Gln Glu Cys Val Ser Glu Tyr Ile Ser Phe Ile
65 70 75 80

Thr Gly Glu Ala Asn Glu Arg Cys Gln Arg Glu Gln Arg Lys Thr Ile
85 90 95

Thr Ala Glu Asp Val Leu Trp Ala Met Ser Arg Leu Gly Phe Asp Asp
100 105 110

Tyr Val Glu Pro Leu Gly Ala Tyr Leu His Arg Tyr Arg Glu Phe Glu
115 120 125

Gly Asp Ala Arg Gly Val Gly Leu Val Pro Gly Ala Ala Pro Ser Arg
130 135 140

Gly Gly Asp His His Pro His Ser Met Ser Pro Ala Ala Met Leu Lys
145 150 155 160

Ser Arg Gly Pro Val Ser Gly Ala Ala Met Leu Pro His His His His
165 170 175

His His Asp Met Gln Met His Ala Ala Met Tyr Gly Gly Thr Ala Val
180 185 190

Pro Pro Pro Ala Gly Pro Pro His His Gly Gly Phe Leu Met Pro His
195 200 205

Pro Gln Gly Ser Ser His Tyr Leu Pro Tyr Ala Tyr Glu Pro Thr Tyr
210 215 220

Gly Gly Glu His Ala Met Ala Ala Tyr Tyr Gly Gly Ala Ala Tyr Ala
225 230 235 240

Pro Gly Asn Gly Gly Ser Gly Asp Gly Ser Gly Ser Gly Gly Gly Gly
245 250 255

Gly Ser Ala Ser His Thr Pro Gln Gly Ser Gly Gly Leu Glu His Pro
260 265 270

His Pro Phe Ala Tyr Lys
275



135
1269
DNA
Zea mays

135

ccacgcgtcc gcatgaataa tccccaaaac cctaaagcca gtgctccttg caccttgcca 60

ccggagcttc ccaaagaagc agtggcgacc gacgaagcac cgccgccaat gggcaacaac 120

aacaacacgg aatcggcgac ggcgacgatg gtccgggagc aggaccggct gatgcccgtg 180

gccaacgtgt cccgcatcat gcgccaagtg ctgcctccgt acgccaagat ctccgacgac 240

gccaaggagg tgatccagga gtgcgtgtcg gagttcatca gcttcgtcac tggcgaggcg 300

aacgagcggt gccacaccga gcgccgcaag accgtcacct ccgaggacat cgtgtgggcc 360

atgagccgcc tcggcttcga cgactacgtc gcgcccctcg gcgccttcct ccagcgcatg 420

cgcgacgaca gcgaccacgg cggtgaagag cgcggcggcc ctgcagggcg tggtggctcg 480

cgccgcggct cgtcgtcctt gccgctccac tgcccgcagc agatgcacca cctgcaccca 540

gccgtctgcc ggcgtccgca ccagagcgtg tcgcctgctg caggatacgc cgtccggccc 600

gttccccgcc cgatgccagc cagtgggtac cgcatgcagg gcggagacca ccgcagcgtg 660

ggcggcgtgg ctccctgcag ctacggaggg gcgctcgtcc aggccggtgg aacccaacac 720

gttgttggat tccacgacga cgaggcaagc tcttcgagtg aaaatccgcc gccggagggg 780

cgtgccgctg gctcgaacta gcctagcttc tcagttcccc gtgtacaata agaggggcgg 840

tcgcggcgcc gcgccgcgcc cttgggttgg gccgggcgct atgctgcagt ttggtttgta 900

aactaacgag cctagggtag ctggtgcacg cgcgccacct cgccggacgt cgccgtcgtc 960

gtcggcatgg acttaaccgg cgggccctgt tgttatttct caagtttgta gccaacgcac 1020

tgttcggtgc gttccataat ttaatttacc atgttgctct cgaaatgaaa aaaaaaaaaa 1080

aaaaaagggc ggccgccctt tttttttttt tttttttttt tcctcttaag gcaaggcaac 1140

tcctgtttgt aggggaatcg ttatggttct gcttctgatt gctcctagtt cttccatcat 1200

tttcgtgttc aaagagaagg ctcccagaaa ataaaataac gattgctatg aaaaaaaaaa 1260

aaaaaaaag 1269



136
262
PRT
Zea mays

136

Met Asn Asn Pro Gln Asn Pro Lys Ala Ser Ala Pro Cys Thr Leu Pro
1 5 10 15

Pro Glu Leu Pro Lys Glu Ala Val Ala Thr Asp Glu Ala Pro Pro Pro
20 25 30

Met Gly Asn Asn Asn Asn Thr Glu Ser Ala Thr Ala Thr Met Val Arg
35 40 45

Glu Gln Asp Arg Leu Met Pro Val Ala Asn Val Ser Arg Ile Met Arg
50 55 60

Gln Val Leu Pro Pro Tyr Ala Lys Ile Ser Asp Asp Ala Lys Glu Val
65 70 75 80

Ile Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Val Thr Gly Glu Ala
85 90 95

Asn Glu Arg Cys His Thr Glu Arg Arg Lys Thr Val Thr Ser Glu Asp
100 105 110

Ile Val Trp Ala Met Ser Arg Leu Gly Phe Asp Asp Tyr Val Ala Pro
115 120 125

Leu Gly Ala Phe Leu Gln Arg Met Arg Asp Asp Ser Asp His Gly Gly
130 135 140

Glu Glu Arg Gly Gly Pro Ala Gly Arg Gly Gly Ser Arg Arg Gly Ser
145 150 155 160

Ser Ser Leu Pro Leu His Cys Pro Gln Gln Met His His Leu His Pro
165 170 175

Ala Val Cys Arg Arg Pro His Gln Ser Val Ser Pro Ala Ala Gly Tyr
180 185 190

Ala Val Arg Pro Val Pro Arg Pro Met Pro Ala Ser Gly Tyr Arg Met
195 200 205

Gln Gly Gly Asp His Arg Ser Val Gly Gly Val Ala Pro Cys Ser Tyr
210 215 220

Gly Gly Ala Leu Val Gln Ala Gly Gly Thr Gln His Val Val Gly Phe
225 230 235 240

His Asp Asp Glu Ala Ser Ser Ser Ser Glu Asn Pro Pro Pro Glu Gly
245 250 255

Arg Ala Ala Gly Ser Asn
260



137
481
DNA
Argemone mexicana

unsure
(410)
n = A, C, G, or T


137

cgagagaaag agttggtgaa gaagaagaag aagttgaaaa gagatggaac gtggtggtgg 60

tggtggtggt agtggtggtg gtttccatgg atatcagaaa ctcccaaaat caaactccgc 120

tggaatgatg ctctcggagc tatcgaataa caacaacaat attgacgtaa actctacatg 180

tactgtacga gagcaagatc gatacatgcc aattgctaat gtgatcagga tcatgcgtaa 240

ggtacttcct actcatgcca agatctctga cgatgccaaa gaaactatcc aagaatgtgt 300

ctcagaatac atcagtttca tcacaagtga agccaatgat cgttgccaac gtgaacaaag 360

aaagacaatc acagctgaag atgttttatg ggcgatgagc aaactagggn ttgatgagta 420

cattgaacct ctaactcttt accttcaacg ttatcgtgag tttgaaggtg nacgttggtc 480

a 481



138
146
PRT
Argemone mexicana

UNSURE
(123)
Xaa = any amino acid


138

Met Glu Arg Gly Gly Gly Gly Gly Gly Ser Gly Gly Gly Phe His Gly
1 5 10 15

Tyr Gln Lys Leu Pro Lys Ser Asn Ser Ala Gly Met Met Leu Ser Glu
20 25 30

Leu Ser Asn Asn Asn Asn Asn Ile Asp Val Asn Ser Thr Cys Thr Val
35 40 45

Arg Glu Gln Asp Arg Tyr Met Pro Ile Ala Asn Val Ile Arg Ile Met
50 55 60

Arg Lys Val Leu Pro Thr His Ala Lys Ile Ser Asp Asp Ala Lys Glu
65 70 75 80

Thr Ile Gln Glu Cys Val Ser Glu Tyr Ile Ser Phe Ile Thr Ser Glu
85 90 95

Ala Asn Asp Arg Cys Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu
100 105 110

Asp Val Leu Trp Ala Met Ser Lys Leu Gly Xaa Asp Glu Tyr Ile Glu
115 120 125

Pro Leu Thr Leu Tyr Leu Gln Arg Tyr Arg Glu Phe Glu Gly Xaa Arg
130 135 140

Trp Ser
145



139
1154
DNA
Glycine max

unsure
(3)
n = A, C, G, or T


139

atnacacaca cctaccttat aactatggaa actggaggct ttcatggcta ccgcaagctc 60

cccaacacaa cctctgggtt gaagctgtca gtgtcagaca tgaacatgaa catgaggcag 120

cagcaggtag catcatcaga tcagaactgc agcaaccaca gtgcagcagg agaggagaac 180

gaatgcacgg tgagggagca agacaggttc atgccaatcg ctaacgtgat acggatcatg 240

cgcaagattc tccctccaca cgcaaaaatc tccgatgatg caaaggagac aatccaagag 300

tgcgtgtcgg agtacatcag cttcatcacc ggggaggcca acgagcgttg ccagagggag 360

cagcgcaaga ccataaccgc agaggacgtg ctttgggcaa tgagtaagct tggattcgac 420

gactacatcg aaccgttaac catgtacctt caccgctacc gtgagctgga gggtgaccgc 480

acctctatga ggggtgaacc gctcgggaag aggactgtgg aatatgccac gcttgctact 540

gcttttgtgc cgccaccctt tcatcaccac aatggctact ttggtgctgc catgcccatg 600

gggacttacg ttagggaaac gccaccaaat gctgcgtcat ctcatcacca tcatggaatc 660

tccaatgctc atgaaccaaa tgctcgctcc atataaaatt aatgaagagt actgttcagt 720

aggagaacaa gacttcttgg acttgattag cttaactctc agtgattggt gttagagtac 780

tgttgttgag gatggttaat tttataatta agggctggga attggggagt tagtatatat 840

tcctaatcct aattatgtgc atctttaatt tatggaataa ctttgttttt tgttttaact 900

tctgataatt tggattttct gatgtttaat gtggttttgt ctatccctta ttaacagtgc 960

caagcttaag gttttagcca tgctccaaaa tggaatactt gtactgttat gttgttctgg 1020

tagtgatggt gatgaaacct gcaagttatg tttatgtata aagccactat tgatcaaaat 1080

tagagaaatt atcatttaat aagtatcctc ccatgttaat tttaaaaaaa aaaaaaaaaa 1140

actcgagacc ggca 1154



140
223
PRT
Glycine max

140

Met Glu Thr Gly Gly Phe His Gly Tyr Arg Lys Leu Pro Asn Thr Thr
1 5 10 15

Ser Gly Leu Lys Leu Ser Val Ser Asp Met Asn Met Asn Met Arg Gln
20 25 30

Gln Gln Val Ala Ser Ser Asp Gln Asn Cys Ser Asn His Ser Ala Ala
35 40 45

Gly Glu Glu Asn Glu Cys Thr Val Arg Glu Gln Asp Arg Phe Met Pro
50 55 60

Ile Ala Asn Val Ile Arg Ile Met Arg Lys Ile Leu Pro Pro His Ala
65 70 75 80

Lys Ile Ser Asp Asp Ala Lys Glu Thr Ile Gln Glu Cys Val Ser Glu
85 90 95

Tyr Ile Ser Phe Ile Thr Gly Glu Ala Asn Glu Arg Cys Gln Arg Glu
100 105 110

Gln Arg Lys Thr Ile Thr Ala Glu Asp Val Leu Trp Ala Met Ser Lys
115 120 125

Leu Gly Phe Asp Asp Tyr Ile Glu Pro Leu Thr Met Tyr Leu His Arg
130 135 140

Tyr Arg Glu Leu Glu Gly Asp Arg Thr Ser Met Arg Gly Glu Pro Leu
145 150 155 160

Gly Lys Arg Thr Val Glu Tyr Ala Thr Leu Ala Thr Ala Phe Val Pro
165 170 175

Pro Pro Phe His His His Asn Gly Tyr Phe Gly Ala Ala Met Pro Met
180 185 190

Gly Thr Tyr Val Arg Glu Thr Pro Pro Asn Ala Ala Ser Ser His His
195 200 205

His His Gly Ile Ser Asn Ala His Glu Pro Asn Ala Arg Ser Ile
210 215 220



141
942
DNA
Glycine max

141

gcacgagctc tcttataatc acacacacac ctaccttaat agctatggaa actggaggct 60

ttcacggcta ccgcaagctc cccaacacca ccgctgggtt gaagctgtca gtgtcagaca 120

tgaacatgag gcagcaggta gcatcatcag atcacagtgc agccacagga gaggagaacg 180

aatgcacggt gagggagcaa gacaggttca tgccaatcgc caacgtgatt aggatcatgc 240

gcaagattct ccctccacac gcaaaaatct cggacgatgc aaaagaaaca atccaagagt 300

gcgtgtctga gtacatcagc ttcatcacag gtgaggcgaa cgagcgttgc cagagggagc 360

agcggaagac cataaccgca gaggacgtgc tttgggccat gagcaagctt ggattcgacg 420

actacatcga accgttgacc atgtaccttc accgctaccg tgaacttgag ggtgaccgca 480

cctctatgag gggtgaacca ctcgggaaga ggactgtgga atacgccacg cttggtgttg 540

ctactgcttt tgtccctcca ccctatcatc accacaatgg gtactttggt gctgccatgc 600

ccatggggac ttacgttagg gaagcgccac caaatacagc ctcctcccat caccaccacc 660

accaccacca ccaccatgct cgtggaatct ccaatgctca tgaaccaaat gctcgctcca 720

tataaaatta tataattatg actaggattc agaacaagac ttgatgatga ttagcttaac 780

tctcagtaat tggtgctaga gtactactgt tgttgaggat actttatttt ataattaagg 840

gctgggaagg gagttagtat attcctaatc ctaactatgt gcatctttaa tttatgaaat 900

cactttgttt taacctttga tgaaaaaaaa aaaaaaaaaa aa 942



142
240
PRT
Glycine max

142

Thr Ser Ser Leu Ile Ile Thr His Thr Pro Thr Leu Ile Ala Met Glu
1 5 10 15

Thr Gly Gly Phe His Gly Tyr Arg Lys Leu Pro Asn Thr Thr Ala Gly
20 25 30

Leu Lys Leu Ser Val Ser Asp Met Asn Met Arg Gln Gln Val Ala Ser
35 40 45

Ser Asp His Ser Ala Ala Thr Gly Glu Glu Asn Glu Cys Thr Val Arg
50 55 60

Glu Gln Asp Arg Phe Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg
65 70 75 80

Lys Ile Leu Pro Pro His Ala Lys Ile Ser Asp Asp Ala Lys Glu Thr
85 90 95

Ile Gln Glu Cys Val Ser Glu Tyr Ile Ser Phe Ile Thr Gly Glu Ala
100 105 110

Asn Glu Arg Cys Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu Asp
115 120 125

Val Leu Trp Ala Met Ser Lys Leu Gly Phe Asp Asp Tyr Ile Glu Pro
130 135 140

Leu Thr Met Tyr Leu His Arg Tyr Arg Glu Leu Glu Gly Asp Arg Thr
145 150 155 160

Ser Met Arg Gly Glu Pro Leu Gly Lys Arg Thr Val Glu Tyr Ala Thr
165 170 175

Leu Gly Val Ala Thr Ala Phe Val Pro Pro Pro Tyr His His His Asn
180 185 190

Gly Tyr Phe Gly Ala Ala Met Pro Met Gly Thr Tyr Val Arg Glu Ala
195 200 205

Pro Pro Asn Thr Ala Ser Ser His His His His His His His His His
210 215 220

His Ala Arg Gly Ile Ser Asn Ala His Glu Pro Asn Ala Arg Ser Ile
225 230 235 240



143
796
DNA
Glycine max

143

gcacgagcaa tggcgggagt gagggaacag gaccagtaca tgccgatagc gaacgtgata 60

aggatcatgc gtcggattct gccagcgcac gcgaagatct cagacgacgc gaaggagacg 120

atccaggagt gcgtgtctga gtacatcagt ttcatcacgg cggaggcgaa cgagcggtgc 180

cagcgggagc agcggaagac ggtgaccgca gaggatgtgt tgtgggcgat ggagaagctt 240

ggctttgaca actacgctca ccctctctct ctttaccttc accgctaccg cgagagtgaa 300

ggagaacctg cttctgtcag acgcgcttct tctgcaatgg ggatcaataa taatatggtg 360

cacccacctt atattaattc tcatggcttt ggaatgtttg attttgaccc atcatcgcaa 420

gggttttaca gggacgatca taacgctgct tctggatctg gtggttttgt tgcgcctttt 480

gatccttatg ctaacatcaa acgtgatgcc ctgtgatcat gtaagaacaa caactagtgc 540

atgctgcttt ttcacttggt tagttatatt caagcacaag cacatgcagg tgcagctgca 600

actatttagc ttcatctaca aatctttttt cctctcttct tctcatgctt taattattta 660

gagacaatac ttgttattca ttgttatgct caattgctag cttctattca tcgtcgactg 720

tctgtattgt tgatgttcat tacagtaaca gataagatgg taactgcttt actacttcaa 780

aaaaaaaaaa aaaaaa 796



144
171
PRT
Glycine max

144

Ala Arg Ala Met Ala Gly Val Arg Glu Gln Asp Gln Tyr Met Pro Ile
1 5 10 15

Ala Asn Val Ile Arg Ile Met Arg Arg Ile Leu Pro Ala His Ala Lys
20 25 30

Ile Ser Asp Asp Ala Lys Glu Thr Ile Gln Glu Cys Val Ser Glu Tyr
35 40 45

Ile Ser Phe Ile Thr Ala Glu Ala Asn Glu Arg Cys Gln Arg Glu Gln
50 55 60

Arg Lys Thr Val Thr Ala Glu Asp Val Leu Trp Ala Met Glu Lys Leu
65 70 75 80

Gly Phe Asp Asn Tyr Ala His Pro Leu Ser Leu Tyr Leu His Arg Tyr
85 90 95

Arg Glu Ser Glu Gly Glu Pro Ala Ser Val Arg Arg Ala Ser Ser Ala
100 105 110

Met Gly Ile Asn Asn Asn Met Val His Pro Pro Tyr Ile Asn Ser His
115 120 125

Gly Phe Gly Met Phe Asp Phe Asp Pro Ser Ser Gln Gly Phe Tyr Arg
130 135 140

Asp Asp His Asn Ala Ala Ser Gly Ser Gly Gly Phe Val Ala Pro Phe
145 150 155 160

Asp Pro Tyr Ala Asn Ile Lys Arg Asp Ala Leu
165 170



145
905
DNA
Vernonia mespilifolia

145

gcacgagcca atttctagag agagaacgag agagaattct ctaaagagga aaaatagatg 60

gaacgtggag gaggtttcca tggctaccac aggctcccca tccaccctac atctggaatc 120

caacaatcgg atatgaagct aaagctacca gaaatgacca acaataactc gtccactgat 180

gacaatgagt gcaccgttcg agaacaggac cgcttcatgc cgatagcaaa cgtgatccgc 240

atcatgcgga agatccttcc tccacatgcc aagatctctg atgatgccaa agagacgatc 300

caagaatgtg tttcagagta cattagcttt gtcacaggcg aggcaaatga ccgctgccag 360

cgtgagcaaa ggaagaccat cacagctgaa gatgtgctct gggctatgag caaactggga 420

tttgatgatt atatcgagcc cttgactgtg tatctccatc gctacaggga gtttgatggt 480

ggcgaacgtg gatccataag gggtgagccc cttgtgaaga ggagtacttc tgatcctggt 540

cactttggga tggcttcttt tgtgcctgct tttcatatgg gtcatcataa cggcttcttt 600

ggtcctgcaa gcattggtgg tttcctgaaa gacccatcga gtgctggccc ttcgggacct 660

gcagtcgctg ggtttgagcc gtatgctcag tgtaaagagt aactgcaaaa agtaggggtt 720

gggatgagat gatgatgatg gtggtggtgg tggtggtttg ttttgttttg ttctttcttt 780

tttttttctt ctttcttttc ttggtcattg aggaacaaac ttacattggt tcactttggc 840

taggcatgta aacggttaac atgcttatca agtagtagtt ttcgatcaaa aaaaaaaaaa 900

aaaaa 905



146
214
PRT
Vernonia mespilifolia

146

Met Glu Arg Gly Gly Gly Phe His Gly Tyr His Arg Leu Pro Ile His
1 5 10 15

Pro Thr Ser Gly Ile Gln Gln Ser Asp Met Lys Leu Lys Leu Pro Glu
20 25 30

Met Thr Asn Asn Asn Ser Ser Thr Asp Asp Asn Glu Cys Thr Val Arg
35 40 45

Glu Gln Asp Arg Phe Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg
50 55 60

Lys Ile Leu Pro Pro His Ala Lys Ile Ser Asp Asp Ala Lys Glu Thr
65 70 75 80

Ile Gln Glu Cys Val Ser Glu Tyr Ile Ser Phe Val Thr Gly Glu Ala
85 90 95

Asn Asp Arg Cys Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu Asp
100 105 110

Val Leu Trp Ala Met Ser Lys Leu Gly Phe Asp Asp Tyr Ile Glu Pro
115 120 125

Leu Thr Val Tyr Leu His Arg Tyr Arg Glu Phe Asp Gly Gly Glu Arg
130 135 140

Gly Ser Ile Arg Gly Glu Pro Leu Val Lys Arg Ser Thr Ser Asp Pro
145 150 155 160

Gly His Phe Gly Met Ala Ser Phe Val Pro Ala Phe His Met Gly His
165 170 175

His Asn Gly Phe Phe Gly Pro Ala Ser Ile Gly Gly Phe Leu Lys Asp
180 185 190

Pro Ser Ser Ala Gly Pro Ser Gly Pro Ala Val Ala Gly Phe Glu Pro
195 200 205

Tyr Ala Gln Cys Lys Glu
210



147
1098
DNA
Triticum aestivum

147

gcacgagcaa gtgcgagtgc gactacctgc attgcacctt ggctagccct agacatggag 60

aacgacggcg tccccaacgg accagcggcg ccggcaccta cccaggggac gccggtggtg 120

cgggagcagg accggctgat gccgatcgcg aacgtgatcc gcatcatgcg ccgtgcgctc 180

cctgcccacg ccaagatctc cgacgacgcc aaggaggcga ttcaggaatg cgtgtccgag 240

ttcatcagct tcgtcaccgg cgaggccaac gaacggtgcc gcatgcagca ccgcaagacc 300

gtcaacgccg aagacatcgt gtgggcccta aaccgcctcg gcttcgacga ctacgtcgtg 360

cccctcagcg tcttcctgca ccgcatgcgc gaccccgagg cggggacagg tggtgccgct 420

gcaggcgaca gccgcgccgt gacgagtgcg cctccccgcg cggccccgcc cgtgatccac 480

gccgtgccgc tgcaggctca gcgcccgatg tacgcgcccc cggctccgtt gcaggttgag 540

aatcagatgc agcggcctgt gtacgctccc ccggctccgg tgcaggttca gatgcagcgg 600

ggcatctatg ggccccgggc tccagtgcac gggtacgccg tcggaatggc gcccgtgcgg 660

gccaacgtcg gcgggcagta ccaggtgttc ggcggagagg gtgtcatggc ccagcaatac 720

tacgggtacg ggtacgagga aggagcgtac ggcgcaggta gcagcaacgg aggagccgcc 780

attggcgacg aggagagctc gtccaacggc gtgccggcac cgggggaggg catgggggag 840

ccagagccag agccagcagc agaagaatcg catgacaagc ccgtccaatc tggctagtcg 900

cgtgcgcggc gcgcgttagc ttctgcgtcc tgtgtactgt aataatttgc cgtgtcgatc 960

cggccatggt ttgtgtgtgc gtagtgctta tctaatgtgg gcttgtcctc tagtaattca 1020

tgtattgctt atctaatgtg gacttgtcct ctagtaattc atgtactctt tgctgttgaa 1080

aaaaaaaaaa aaaaaaaa 1098



148
280
PRT
Triticum aestivum

148

Met Glu Asn Asp Gly Val Pro Asn Gly Pro Ala Ala Pro Ala Pro Thr
1 5 10 15

Gln Gly Thr Pro Val Val Arg Glu Gln Asp Arg Leu Met Pro Ile Ala
20 25 30

Asn Val Ile Arg Ile Met Arg Arg Ala Leu Pro Ala His Ala Lys Ile
35 40 45

Ser Asp Asp Ala Lys Glu Ala Ile Gln Glu Cys Val Ser Glu Phe Ile
50 55 60

Ser Phe Val Thr Gly Glu Ala Asn Glu Arg Cys Arg Met Gln His Arg
65 70 75 80

Lys Thr Val Asn Ala Glu Asp Ile Val Trp Ala Leu Asn Arg Leu Gly
85 90 95

Phe Asp Asp Tyr Val Val Pro Leu Ser Val Phe Leu His Arg Met Arg
100 105 110

Asp Pro Glu Ala Gly Thr Gly Gly Ala Ala Ala Gly Asp Ser Arg Ala
115 120 125

Val Thr Ser Ala Pro Pro Arg Ala Ala Pro Pro Val Ile His Ala Val
130 135 140

Pro Leu Gln Ala Gln Arg Pro Met Tyr Ala Pro Pro Ala Pro Leu Gln
145 150 155 160

Val Glu Asn Gln Met Gln Arg Pro Val Tyr Ala Pro Pro Ala Pro Val
165 170 175

Gln Val Gln Met Gln Arg Gly Ile Tyr Gly Pro Arg Ala Pro Val His
180 185 190

Gly Tyr Ala Val Gly Met Ala Pro Val Arg Ala Asn Val Gly Gly Gln
195 200 205

Tyr Gln Val Phe Gly Gly Glu Gly Val Met Ala Gln Gln Tyr Tyr Gly
210 215 220

Tyr Gly Tyr Glu Glu Gly Ala Tyr Gly Ala Gly Ser Ser Asn Gly Gly
225 230 235 240

Ala Ala Ile Gly Asp Glu Glu Ser Ser Ser Asn Gly Val Pro Ala Pro
245 250 255

Gly Glu Gly Met Gly Glu Pro Glu Pro Glu Pro Ala Ala Glu Glu Ser
260 265 270

His Asp Lys Pro Val Gln Ser Gly
275 280



149
932
DNA
Canna edulis

149

gcaccagctc aaatctccga attagggttt ctgtgccttg tctccaatgg cggaatcggg 60

ggccccgggc acgcccgaga gcggacattc cggcggcgga tctggcgcgc gggagcagga 120

ccgctgcctc cccattgcca acattgggcg gattatgagg aaggccgtac ccgagaacgg 180

caagatcgcc aaggacgcca aggaatccgt ccaggagtgc gtctccgagt tcatcagctt 240

cgtcaccagc gaggcgagcg ataagtgccg ccgcgagaaa aggaagacga tcaacggcga 300

tgatcttctg tgggctatgc ggatgcttgg cttcgaagag tacgtcgagc ctcttaagct 360

ctacttgcag ctctacagag agatggaggg aaacgtcatg gtttcacgtc ccgctgatca 420

atgatcaacc aggaaaaaga gatggagcaa ttaacaggca gcccacagat tcgttcaatg 480

gcatgtagga tggttctcaa gaaagcaaac ttttgcttac tatttcaagg tgtaggccct 540

ttgttagtgt agttaataag ttatagttgc tgcaggttat ttttgttctt atttgtactc 600

ttgtccaata ccttttcctc taagtgaaca acattcagag aatggctctt ctctaggact 660

tggacgaagg cacgaagcac tgatctgaag ttatgatcca ttcaaccatc taaaattaat 720

tttaaatttt aaattgagac aatgttttga cccttgtttc gacatttccc gacagcccta 780

ctgtaatgta aagatgactt ggatagcaaa attgttaaaa aggtacaatt cctgcaatgt 840

tttacaagtc aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 900

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 932



150
121
PRT
Canna edulis

150

Met Ala Glu Ser Gly Ala Pro Gly Thr Pro Glu Ser Gly His Ser Gly
1 5 10 15

Gly Gly Ser Gly Ala Arg Glu Gln Asp Arg Cys Leu Pro Ile Ala Asn
20 25 30

Ile Gly Arg Ile Met Arg Lys Ala Val Pro Glu Asn Gly Lys Ile Ala
35 40 45

Lys Asp Ala Lys Glu Ser Val Gln Glu Cys Val Ser Glu Phe Ile Ser
50 55 60

Phe Val Thr Ser Glu Ala Ser Asp Lys Cys Arg Arg Glu Lys Arg Lys
65 70 75 80

Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Arg Met Leu Gly Phe
85 90 95

Glu Glu Tyr Val Glu Pro Leu Lys Leu Tyr Leu Gln Leu Tyr Arg Glu
100 105 110

Met Glu Gly Asn Val Met Val Ser Arg
115 120



151
863
DNA
Momordica charantia

151

gcacgagcag gatctcgctc acatggcgga ggctccgacg agtccagccg gcggcagcca 60

cgagagcggc ggcgagcaga gccccaatac cggtggggtt cgggagcagg accgatacct 120

cccgatcgct aacattagcc ggatcatgaa gaaggccttg cccgctaatg gcaagatcgc 180

caaggacgcc aaggacaccg tccaggaatg cgtctccgaa ttcatcagct tcatcactag 240

cgaggcgagc gataagtgcc agaaggagaa gagaaagacc attaatgggg atgatttgct 300

gtgggcaatg gcgacattgg gtttcgagga ctatattgat ccgcttaagt cgtatctaac 360

taggtacaga gagttggagt gtgatgctaa gggatcttct aggggtggtg atgagtctgc 420

taaaagagat gcagttgggg ccttgcctgg ccaaaattcc cagcagtaca tgcagccggg 480

agcaatgacc tacattaaca cccaaggaca gcatttgatc attccttcaa tgcagaataa 540

tgaataggag actcctgcat tccctcttgg attgtctgaa atctgaggct ggtagaagcg 600

ttcaacacct atatagcatc tttacaatcg atttggctaa tttattatga aatgatgata 660

ttatatatat ttctggggtt tctgtgttgg ttctggattt gattttggtt tgggctttta 720

aggtgggctt cgattttatt gatgctctcg tcatctaaag ttattgtaaa tttgggacct 780

tcaatttagt atagttgctt tggtaatttg gaaactggaa aaaaaaaaaa aaaaaaaaaa 840

aaaaaaaaaa aaaaaaaaaa aaa 863



152
174
PRT
Momordica charantia

152

Met Ala Glu Ala Pro Thr Ser Pro Ala Gly Gly Ser His Glu Ser Gly
1 5 10 15

Gly Glu Gln Ser Pro Asn Thr Gly Gly Val Arg Glu Gln Asp Arg Tyr
20 25 30

Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala Leu Pro Ala
35 40 45

Asn Gly Lys Ile Ala Lys Asp Ala Lys Asp Thr Val Gln Glu Cys Val
50 55 60

Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala Ser Asp Lys Cys Gln
65 70 75 80

Lys Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met
85 90 95

Ala Thr Leu Gly Phe Glu Asp Tyr Ile Asp Pro Leu Lys Ser Tyr Leu
100 105 110

Thr Arg Tyr Arg Glu Leu Glu Cys Asp Ala Lys Gly Ser Ser Arg Gly
115 120 125

Gly Asp Glu Ser Ala Lys Arg Asp Ala Val Gly Ala Leu Pro Gly Gln
130 135 140

Asn Ser Gln Gln Tyr Met Gln Pro Gly Ala Met Thr Tyr Ile Asn Thr
145 150 155 160

Gln Gly Gln His Leu Ile Ile Pro Ser Met Gln Asn Asn Glu
165 170



153
1179
DNA
Eucalyptus grandis

153

gcaccagttt ccccccgccc ccccgatcgc cgcccctccc gccggggccg gcggcggcgg 60

ggcgtcggcg gcggcggcgg aggatgtggg gagctttctc acggaggatg aggtttcttc 120

tcttctatgt tttttttttt gcagctgctc ggcttgcctg ccctctcggg cgacgacgcg 180

atggcggagg ctccggcgag tcccggcggc ggcggcagcc acgagagcgg cgagcacagc 240

ccccggtccg gcggcgccgt ccgcgagcag gacaggtacc tccccatcgc caacatcagc 300

cgcatcatga agaaggccct ccccgccaac ggcaagatcg ccaaggacgc caaggagacc 360

gtgcaggagt gcgtctccga gttcatcagc ttcatcacca gcgaggcgag cgacaagtgc 420

cagagggaga agaggaagac gatcaacggc gacgacttgc tctggcccat ggcgacctta 480

gggtttgagg attacctcga tccgcttaag atttacctgg ccagatacag ggagatggag 540

ggggatacca aggggtcagc taaagtgggg gaagcatcta ctaaaagaga tggcgccgca 600

gttcagtcag ttcctaatgc acagattgct catcaaggtt ctttctctca cggcaccaac 660

tattcgcatt ctcaagttca ccatcctgcg cttccgatgc atggctcaga atgacatgtt 720

ccagcccttg ttgcatgaga tgaagaagtc atcacacttg ttccaggcgt ttgactcatc 780

tcggcatcaa gatattcata agatgtgctg ctgacatttt agggtggtct ctgccaattg 840

tgttcatttg gagttgtttt ccagtgggct gtatatttta gcatctgcat catatttgct 900

ttcagcctta catatgtctg gtttagattt acttgataat gtagaaaggt aagcccccct 960

gcgagtattt atcttattgt catttagatt cgacacccaa ggaggacgag aatgaagttt 1020

ctttttagct ctctgtttcg ttggagttgt cttgtgtatt cttgagttag aaacttgtga 1080

acaaattggt atgcacagtc catgtttatg tgacaatgtc gaggtctgag tgtataatcc 1140

agagtccaat tcagatcgta aaaaaaaaaa aaaaaaaaa 1179



154
177
PRT
Eucalyptus grandis

154

Met Ala Glu Ala Pro Ala Ser Pro Gly Gly Gly Gly Ser His Glu Ser
1 5 10 15

Gly Glu His Ser Pro Arg Ser Gly Gly Ala Val Arg Glu Gln Asp Arg
20 25 30

Tyr Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala Leu Pro
35 40 45

Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln Glu Cys
50 55 60

Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala Ser Asp Lys Cys
65 70 75 80

Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Pro
85 90 95

Met Ala Thr Leu Gly Phe Glu Asp Tyr Leu Asp Pro Leu Lys Ile Tyr
100 105 110

Leu Ala Arg Tyr Arg Glu Met Glu Gly Asp Thr Lys Gly Ser Ala Lys
115 120 125

Val Gly Glu Ala Ser Thr Lys Arg Asp Gly Ala Ala Val Gln Ser Val
130 135 140

Pro Asn Ala Gln Ile Ala His Gln Gly Ser Phe Ser His Gly Thr Asn
145 150 155 160

Tyr Ser His Ser Gln Val His His Pro Ala Leu Pro Met His Gly Ser
165 170 175

Glu



155
983
DNA
Zea mays

155

gcacgagccg gagcgcctcc tcttctccag cgtccgatcc ccattcccca cctctcctcc 60

ctccgccgcc agctcccgcc cccttctctc ccctcctcgc ctccccgcgc gcgcgttttt 120

ataagggttt cggcggaggc gcccggtcgc tggcgatggc cgacgacggc gggagccacg 180

agggcagcgg cggcggcgga ggcgtccggg agcaggaccg gttcctgccc atcgccaaca 240

tcagccggat catgaagaag gccgtcccgg ccaacggcaa gatcgccaag gacgctaagg 300

agaccctgca ggagtgcgtc tccgagttca tatcattcgt gaccagcgag gccagcgaca 360

aatgccagaa ggagaaacga aagacaatca acggggacga tttgctctgg gcgatggcca 420

ctttaggatt cgaggagtac gtcgagcctc tcaagattta cctacaaaag tacaaagaga 480

tggagggtga tagcaagctg tctacaaagg ctggcgaggg ctctgtaaag aaggatgcaa 540

ttagtcccca tggtggcacc agtagctcaa gtaatcagtt ggttcagcat ggagtctaca 600

accaagggat gggctatatg cagccacagt accacaatgg ggaaacctaa taaagggcta 660

atacagcagc aatttatgct agggaagtct ctgcattgct taccatgtgt attggcagaa 720

aacaggaggc acttacaaag ggtgttaatc tctgcgatgg ctgcctctca ggtgtaaatt 780

ggcttcggtt tagcgctgct tttgtccgta tatttaggat gatttgactg ttgctacttt 840

tggcaacctt ttacatttac agatatgtat tattcagcat aaatataata tagtagtcct 900

aggcctaaat aatggtgatt aacataccaa gtcttttatc aggctactcg ttttctggaa 960

caaaaaaaaa aaaaaaaaaa aaa 983



156
164
PRT
Zea mays

156

Met Ala Asp Asp Gly Gly Ser His Glu Gly Ser Gly Gly Gly Gly Gly
1 5 10 15

Val Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile
20 25 30

Met Lys Lys Ala Val Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys
35 40 45

Glu Thr Leu Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Val Thr Ser
50 55 60

Glu Ala Ser Asp Lys Cys Gln Lys Glu Lys Arg Lys Thr Ile Asn Gly
65 70 75 80

Asp Asp Leu Leu Trp Ala Met Ala Thr Leu Gly Phe Glu Glu Tyr Val
85 90 95

Glu Pro Leu Lys Ile Tyr Leu Gln Lys Tyr Lys Glu Met Glu Gly Asp
100 105 110

Ser Lys Leu Ser Thr Lys Ala Gly Glu Gly Ser Val Lys Lys Asp Ala
115 120 125

Ile Ser Pro His Gly Gly Thr Ser Ser Ser Ser Asn Gln Leu Val Gln
130 135 140

His Gly Val Tyr Asn Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His
145 150 155 160

Asn Gly Glu Thr



157
1021
DNA
Zea mays

157

ggcacgagcg ctcctgttct tctcgcatcc ccagcccagg tggtgtcccc tgtcgcgttg 60

atgcatgctc cctcggcggt ggccttgagc tgaggcggcg gagcgatgcc ggactcggac 120

aacgactccg gcgggccgag caacgccggg ggcgagctgt cgtcgccgcg ggagcaggac 180

cggttcctgc ccatcgccaa cgtgagccgg atcatgaaga aggcgctccc ggccaacgcc 240

aagatcagca aggacgccaa ggagacggtg caggagtgcg tgtccgagtt catctccttc 300

atcaccggcg aggcctccga caagtgccag cgcgagaagc gcaagaccat caacggcgac 360

gacctgctgt gggccatgac cacgctcggc ttcgaggact acgtcgagcc gctcaagcac 420

tacctgcaca agttccgcga gatcgagggc gagagggccg ccgcgtccgc cggcgcctcg 480

ggctcgcagc agcagcagca gcagggcgag ctgcccagag gcgccgccaa tgccgccggg 540

tacgccgggt acggcgcgcc tggctccggc ggcatgatga tgatgatgat ggggcagccc 600

atgtacggcg gctcgcagcc gcagcaacag ccgccgccgc ctcagccgcc acagcagcag 660

cagcaacatc aacagcatca catggcaata ggaggcagag gaggattcgg ccaacaaggc 720

ggcggcggcg gctcctcgtc gtcgtcaggg cttggccggc aagacagggc gtgagttgcg 780

acgatacgtt cagaatcaga atcgctgata ctcctacgta gaattatacc tcctacctaa 840

ttgatgacac cgcaccgcac ctcgttgtgc tgcctgtcct tgtacgttta ctaattactg 900

ctgcctgtat gtaaatcaaa atctgaggct cccatttcga aacggacggt gaactactct 960

tcccgtttcg tttcatacga gaatcgaact cgttttcaat taaaaaaaaa aaaaaaaaaa 1020

a 1021



158
222
PRT
Zea mays

158

Met Pro Asp Ser Asp Asn Asp Ser Gly Gly Pro Ser Asn Ala Gly Gly
1 5 10 15

Glu Leu Ser Ser Pro Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn
20 25 30

Val Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Ala Lys Ile Ser
35 40 45

Lys Asp Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu Phe Ile Ser
50 55 60

Phe Ile Thr Gly Glu Ala Ser Asp Lys Cys Gln Arg Glu Lys Arg Lys
65 70 75 80

Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Thr Thr Leu Gly Phe
85 90 95

Glu Asp Tyr Val Glu Pro Leu Lys His Tyr Leu His Lys Phe Arg Glu
100 105 110

Ile Glu Gly Glu Arg Ala Ala Ala Ser Ala Gly Ala Ser Gly Ser Gln
115 120 125

Gln Gln Gln Gln Gln Gly Glu Leu Pro Arg Gly Ala Ala Asn Ala Ala
130 135 140

Gly Tyr Ala Gly Tyr Gly Ala Pro Gly Ser Gly Gly Met Met Met Met
145 150 155 160

Met Met Gly Gln Pro Met Tyr Gly Gly Ser Gln Pro Gln Gln Gln Pro
165 170 175

Pro Pro Pro Gln Pro Pro Gln Gln Gln Gln Gln His Gln Gln His His
180 185 190

Met Ala Ile Gly Gly Arg Gly Gly Phe Gly Gln Gln Gly Gly Gly Gly
195 200 205

Gly Ser Ser Ser Ser Ser Gly Leu Gly Arg Gln Asp Arg Ala
210 215 220



159
1055
DNA
Oryza sativa

159

gcacgagctt acatctctct ctctcctctc ttctcttctt cctcccagac tagtcagtct 60

ctcccaagaa cacccactcc tctagtctct ctctcgagag agagaaaatt gatgattctt 120

gggatgattt tgaggcgtct gatttgctga agaggaggag gaggatgccg gactcggaca 180

acgactccgg cgggccgagc aactacgcgg gaggggagct gtcgtcgccg cgggagcagg 240

acaggttcct gccgatcgcg aacgtgagca ggatcatgaa gaaggcgctg ccggcgaacg 300

ccaagatcag caaggacgcc aaggagacgg tgcaggagtg cgtctccgag ttcatctcct 360

tcatcaccgg cgaggcctcc gacaagtgcc agcgcgagaa gcgcaagacc atcaacggcg 420

acgacctgct ctgggccatg accaccctcg gcttcgagga ctacgtcgac cccctcaagc 480

actacctcca caagttccgc gagatcgagg gcgagcgcgc cgccgcctcc accaccggcg 540

ccggcaccag cgccgcctcc accacgccgc cgcagcagca gcacaccgcc aatgccgccg 600

gcggctacgc cgggtacgcc gccccgggag ccggccccgg cggcatgatg atgatgatgg 660

ggcagcccat gtacggctcg ccgccaccgc cgccacagca gcagcagcag caacaccacc 720

acatggcaat gggaggaaga ggcggcttcg gtcatcatcc cggcggcggc ggcggcgggt 780

cgtcgtcgtc gtcggggcac ggtcggcaaa acaggggcgc ttgacatcgc tccgagacga 840

gtagcatgca ccatggtaca tatatacagt aatcagcagc tgttcatttt tctatgatta 900

ctagttgact taagcttgca aatttgctaa tctgagctcc tgagtttttt tttttggtca 960

gcaatttcaa gatggtcaga agctaaattt gtctatttgt tactgataaa ttatttgttc 1020

tctcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaa 1055



160
219
PRT
Oryza sativa

160

Met Pro Asp Ser Asp Asn Asp Ser Gly Gly Pro Ser Asn Tyr Ala Gly
1 5 10 15

Gly Glu Leu Ser Ser Pro Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala
20 25 30

Asn Val Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Ala Lys Ile
35 40 45

Ser Lys Asp Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu Phe Ile
50 55 60

Ser Phe Ile Thr Gly Glu Ala Ser Asp Lys Cys Gln Arg Glu Lys Arg
65 70 75 80

Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Thr Thr Leu Gly
85 90 95

Phe Glu Asp Tyr Val Asp Pro Leu Lys His Tyr Leu His Lys Phe Arg
100 105 110

Glu Ile Glu Gly Glu Arg Ala Ala Ala Ser Thr Thr Gly Ala Gly Thr
115 120 125

Ser Ala Ala Ser Thr Thr Pro Pro Gln Gln Gln His Thr Ala Asn Ala
130 135 140

Ala Gly Gly Tyr Ala Gly Tyr Ala Ala Pro Gly Ala Gly Pro Gly Gly
145 150 155 160

Met Met Met Met Met Gly Gln Pro Met Tyr Gly Ser Pro Pro Pro Pro
165 170 175

Pro Gln Gln Gln Gln Gln Gln His His His Met Ala Met Gly Gly Arg
180 185 190

Gly Gly Phe Gly His His Pro Gly Gly Gly Gly Gly Gly Ser Ser Ser
195 200 205

Ser Ser Gly His Gly Arg Gln Asn Arg Gly Ala
210 215



161
873
DNA
Oryza sativa

161

gtttttggag ggcggcgcgg ggatggcgga cgcggggcac gacgagagcg ggagcccgcc 60

gaggagcggc ggggtgaggg agcaggacag gttcctgccc atcgccaaca tcagccgcat 120

catgaagaag gccgtcccgg cgaacggcaa gatcgccaag gacgccaagg agaccctgca 180

ggagtgcgtc tcggagttca tctccttcgt caccagcgag gcgagcgaca aatgtcagaa 240

ggagaagcgc aagaccatca acggggaaga tctcctcttt gcgatgggta cgcttggctt 300

tgaggagtac gttgatccgt tgaagatcta tttacacaag tacagagaga tggagggtga 360

tagtaagctg tcctcaaagg ctggtgatgg ttcagtaaag aaggatacaa ttggtccgca 420

cagtggcgct agtagctcaa gtgcgcaagg gatggttggg gcttacaccc aagggatggg 480

ttatatgcaa cctcagtatc ataatgggga cacctaaaga tgaggacagt gaaaattttc 540

agtaactggt gtcctctgtg agttattatc catctgttaa ggaagaaccc acattagggc 600

catatttatt agtagaagac taaagcactt gaagggtgtt ggtttagaaa gggtgttaac 660

agttggctgt ggcgattgct tcacagatgt aaattgcttc ataagtggtt taatgcttgt 720

ttttgcctgt atattcagag caattttcac atattggtag ttctgcaatc ttttgcattc 780

ccatacatgt atcaggtggc acaaatctat tgcaagtacc ctagcattga ataatgctgg 840

ttaacatata aaaaaaaaaa aaaaaaaaaa aaa 873



162
164
PRT
Oryza sativa

162

Met Ala Asp Ala Gly His Asp Glu Ser Gly Ser Pro Pro Arg Ser Gly
1 5 10 15

Gly Val Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg
20 25 30

Ile Met Lys Lys Ala Val Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala
35 40 45

Lys Glu Thr Leu Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Val Thr
50 55 60

Ser Glu Ala Ser Asp Lys Cys Gln Lys Glu Lys Arg Lys Thr Ile Asn
65 70 75 80

Gly Glu Asp Leu Leu Phe Ala Met Gly Thr Leu Gly Phe Glu Glu Tyr
85 90 95

Val Asp Pro Leu Lys Ile Tyr Leu His Lys Tyr Arg Glu Met Glu Gly
100 105 110

Asp Ser Lys Leu Ser Ser Lys Ala Gly Asp Gly Ser Val Lys Lys Asp
115 120 125

Thr Ile Gly Pro His Ser Gly Ala Ser Ser Ser Ser Ala Gln Gly Met
130 135 140

Val Gly Ala Tyr Thr Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His
145 150 155 160

Asn Gly Asp Thr



163
799
DNA
Glycine max

163

gcacgagacg aaagcaacgg tgaagatgaa taatgagtga ggcaatccaa tggtgagaaa 60

ggagtccgtg aaagcagaga cttatcgaga aacaacggca cagaaggttc cacgtgggaa 120

gcagataaag gaatattaag cagagagatc caacggacac tgctagtgaa ggcagaagaa 180

gaagattcct ggattgattg tgaagatggc tgagtcggac aacgactcgg gaggggcgca 240

gaacgcggga aacagtggaa acttgagcga gttgtcgcct cgggaacagg accggtttct 300

ccccatagcg aacgtgagca ggatcatgaa gaaggccttg ccggcgaacg cgaagatctc 360

gaaggacgcg aaggagacgg tgcaggaatg cgtgtcggag ttcatcagct tcataacggg 420

tgaggcgtcg gacaagtgcc agagggagaa gcgcaagacc atcaacggcg acgatcttct 480

ctgggccatg acaaccctgg gattcgaaga gtacgtggag cctctgaaga tttacctcca 540

gcgcttccgc gagatggagg gagagaagac cgtggccgcc cgcgactctt ctaaggactc 600

ggcctccgcc tcctcctatc atcagggaca cgtgtacggc tcccctgcct accatcatca 660

agtgcctggg cccacttatc ctgcccctgg tagacccaga tgacgtgctc ctctattcgc 720

cactccctag actttttata ttatattatt taattaaact ctcttctcca ctcaaccttt 780

gcaaaaaaaa aaaaaaaaa 799



164
165
PRT
Glycine max

164

Met Ala Glu Ser Asp Asn Asp Ser Gly Gly Ala Gln Asn Ala Gly Asn
1 5 10 15

Ser Gly Asn Leu Ser Glu Leu Ser Pro Arg Glu Gln Asp Arg Phe Leu
20 25 30

Pro Ile Ala Asn Val Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn
35 40 45

Ala Lys Ile Ser Lys Asp Ala Lys Glu Thr Val Gln Glu Cys Val Ser
50 55 60

Glu Phe Ile Ser Phe Ile Thr Gly Glu Ala Ser Asp Lys Cys Gln Arg
65 70 75 80

Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Thr
85 90 95

Thr Leu Gly Phe Glu Glu Tyr Val Glu Pro Leu Lys Ile Tyr Leu Gln
100 105 110

Arg Phe Arg Glu Met Glu Gly Glu Lys Thr Val Ala Ala Arg Asp Ser
115 120 125

Ser Lys Asp Ser Ala Ser Ala Ser Ser Tyr His Gln Gly His Val Tyr
130 135 140

Gly Ser Pro Ala Tyr His His Gln Val Pro Gly Pro Thr Tyr Pro Ala
145 150 155 160

Pro Gly Arg Pro Arg
165



165
644
DNA
Glycine max

165

gcacgagcag tttctggggc atctcaaaat caatggaaga tattggaggc agttcctcaa 60

acgacaacaa caacaatggt ggcatcatca aggaacagga ccggttgctg ccaatagcca 120

atgttggtcg gctcatgaag cggattcttc ctcagaacgc caaaatctcg aaggaggcga 180

aggagacgat gcaggaatgt gtgtcggagt tcataagctt cgtgacgagt gaggcttcgg 240

agaagtgcag gaaggagagg aggaagacag tgaatggtga tgacatttgt tgggccttgg 300

caacactagg ctttgataac tatgctgaac caatgagaag gtacttgcat agatatagag 360

aggttgaggt agatcataat aaggtcaatc ttcaagaaaa agggaatagt cctgaagaga 420

aagacgatga attatttaaa ttgagcaata gaggggttgg gctttgacca attattatgc 480

ttatagtaga caggaactcg ttaatccatt catactcatc actgattact gattagatga 540

attagtaatt ttaaggtttt tgtgaggatg agataatata tgtaataatt ttcttgtctt 600

aattggaatt tatcgagctt agaacaaaaa aaaaaaaaaa aaaa 644



166
152
PRT
Glycine max

166

Ser Phe Trp Gly Ile Ser Lys Ser Met Glu Asp Ile Gly Gly Ser Ser
1 5 10 15

Ser Asn Asp Asn Asn Asn Asn Gly Gly Ile Ile Lys Glu Gln Asp Arg
20 25 30

Leu Leu Pro Ile Ala Asn Val Gly Arg Leu Met Lys Arg Ile Leu Pro
35 40 45

Gln Asn Ala Lys Ile Ser Lys Glu Ala Lys Glu Thr Met Gln Glu Cys
50 55 60

Val Ser Glu Phe Ile Ser Phe Val Thr Ser Glu Ala Ser Glu Lys Cys
65 70 75 80

Arg Lys Glu Arg Arg Lys Thr Val Asn Gly Asp Asp Ile Cys Trp Ala
85 90 95

Leu Ala Thr Leu Gly Phe Asp Asn Tyr Ala Glu Pro Met Arg Arg Tyr
100 105 110

Leu His Arg Tyr Arg Glu Val Glu Val Asp His Asn Lys Val Asn Leu
115 120 125

Gln Glu Lys Gly Asn Ser Pro Glu Glu Lys Asp Asp Glu Leu Phe Lys
130 135 140

Leu Ser Asn Arg Gly Val Gly Leu
145 150



167
879
DNA
Glycine max

167

gcacgagaag gaacgtgaaa gtaaaacgga cggtggcgat agaagcgtct ctcatctcca 60

tcgtctcctc actcctctct tctccagcgt tcattttttc tcgcgcccaa atacaaaatc 120

acatcacaac agggttccgg cgaccatgtc cgatgctccg gcgagtccat gcggcggcgg 180

cggcggaggc agccacgaga gcggcgagca cagtccccgc tccaatttcc gcgagcagga 240

ccgcttcctc cccatcgcca acatcagccg catcatgaag aaagcgcttc ctcccaacgg 300

gaaaatcgcc aaggacgcca aggaaaccgt gcaggaatgc gtctccgagt tcatcagctt 360

cgtcaccagc gaagcgagcg ataagtgtca gagagagaag aggaagacca tcaacggcga 420

cgatttgctt tgggctatga ccactttagg tttcgaggag tatattgatc cgctcaaggt 480

ttacctcgcc gcttacagag agattgaggg tgattcaaag ggttcggcca agggtggaga 540

tgcatctgct aagagagatg tttatcagag tcctaatggc caggttgctc atcaaggttc 600

tttctcacaa ggtgttaatt atacgaattc ttagccccag gctcaacata tgatagttcc 660

gatgcaaggc caagagtaga tattgatcct ctccttcagt gtttgacatg tgtgatctaa 720

atgccagtgg aacttttatg tcaatatgtg cccttggtat aatgaatgca ttttatgtta 780

tgtaaacact acatgcgggg atgttggttc ttgtgaccag atattattta ttaagactta 840

catttatctt tggaaaaaaa aaaaaaaaaa aaaaaaaaa 879



168
162
PRT
Glycine max

168

Met Ser Asp Ala Pro Ala Ser Pro Cys Gly Gly Gly Gly Gly Gly Ser
1 5 10 15

His Glu Ser Gly Glu His Ser Pro Arg Ser Asn Phe Arg Glu Gln Asp
20 25 30

Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala Leu
35 40 45

Pro Pro Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln Glu
50 55 60

Cys Val Ser Glu Phe Ile Ser Phe Val Thr Ser Glu Ala Ser Asp Lys
65 70 75 80

Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp
85 90 95

Ala Met Thr Thr Leu Gly Phe Glu Glu Tyr Ile Asp Pro Leu Lys Val
100 105 110

Tyr Leu Ala Ala Tyr Arg Glu Ile Glu Gly Asp Ser Lys Gly Ser Ala
115 120 125

Lys Gly Gly Asp Ala Ser Ala Lys Arg Asp Val Tyr Gln Ser Pro Asn
130 135 140

Gly Gln Val Ala His Gln Gly Ser Phe Ser Gln Gly Val Asn Tyr Thr
145 150 155 160

Asn Ser



169
771
DNA
Glycine max

169

gcacgagagt ctttagaaaa gatatccatg gctgagtccg acaacgagtc aggaggtcac 60

acggggaacg cgagcgggag caacgagttg tccggttgca gggagcaaga caggttcctc 120

ccaatagcaa acgtgagcag gatcatgaag aaggcgttgc cggcgaacgc gaagatatcg 180

aaggaggcga aggagacggt gcaggagtgc gtgtcggagt tcatcagctt cataacagga 240

gaggcttccg ataagtgcca gaaggagaag aggaagacga tcaacggcga cgatcttctc 300

tgggccatga ctaccctggg cttcgaggac tacgtggatc ctctcaagat ttacctgcac 360

aagtataggg agatggaggg ggagaaaacc gctatgatgg gaaggccaca tgagagggat 420

gagggttatg gccatggcca tggtcatgca actcctatga tgacgatgat gatggggcat 480

cagccccagc accagcacca gcaccagcac cagcaccagc accagggaca cgtgtatgga 540

tctggatcag catcttctgc aagaactaga tagcatgtgt catctgttta agcttaattg 600

attttattat gaggatgata tgatataaga tttatattcg tatatgtttg gttttagaaa 660

tacaccagct ccagcttgta attgcttgaa acttccttgt tgagagaata tagacattat 720

tgtggatggt gatgtggcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a 771



170
181
PRT
Glycine max

170

Met Ala Glu Ser Asp Asn Glu Ser Gly Gly His Thr Gly Asn Ala Ser
1 5 10 15

Gly Ser Asn Glu Leu Ser Gly Cys Arg Glu Gln Asp Arg Phe Leu Pro
20 25 30

Ile Ala Asn Val Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Ala
35 40 45

Lys Ile Ser Lys Glu Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu
50 55 60

Phe Ile Ser Phe Ile Thr Gly Glu Ala Ser Asp Lys Cys Gln Lys Glu
65 70 75 80

Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Thr Thr
85 90 95

Leu Gly Phe Glu Asp Tyr Val Asp Pro Leu Lys Ile Tyr Leu His Lys
100 105 110

Tyr Arg Glu Met Glu Gly Glu Lys Thr Ala Met Met Gly Arg Pro His
115 120 125

Glu Arg Asp Glu Gly Tyr Gly His Gly His Gly His Ala Thr Pro Met
130 135 140

Met Thr Met Met Met Gly His Gln Pro Gln His Gln His Gln His Gln
145 150 155 160

His Gln His Gln His Gln Gly His Val Tyr Gly Ser Gly Ser Ala Ser
165 170 175

Ser Ala Arg Thr Arg
180



171
848
DNA
Glycine max

171

gcgccaaata caaattcgtg tcaacccaac ccagggttcc ggcgagcatg gccgacggtc 60

cggctagccc aggcggcggc agccacgaga gcggcgacca cagccctcgc tctaacgtgc 120

gcgagcagga caggtacctc cctatcgcta acataagccg catcatgaag aaggcacttc 180

ctgccaacgg taaaatcgca aaggacgcca aagagaccgt tcaggaatgc gtctccgagt 240

tcatcagctt catcaccagc gagttatgtc agagagaaaa gagaaagact attaacggcg 300

atgatttgct ctgggcgatg gccactctcg gtttcgagga ttatatggat cctcttaaaa 360

tttacctcac tagataccga gagatggagg gtgatacgaa gggctctgcc aagggtggag 420

actcatctgc taagagagat gttcagccaa gtcctaatgc tcagcttgct catcaaggtt 480

ctttctcaca aaatgttact tacccgaatt ctcagggtcg acatatgatg gttccaatgc 540

aaggcccgga gtaggtatca agtttattat tgaccctctt gttgtaacgt atgttttcta 600

cgccagttac caagtgctca cggcatattg aatgtctttt tatgttatgt gaatactgac 660

aggagatgtt ggttcttgtg tccgtttttt tttttttaaa ttaaggtttg tatattatct 720

ttggattcga attattattt gaaagttatt attatattgt aaatcctaga gccctgttgt 780

ctgaatccat caggcggctt ggtaaagacc gagattttag gactgattgt aagcataaat 840

ccgaatat 848



172
168
PRT
Glycine max

172

Met Ala Asp Gly Pro Ala Ser Pro Gly Gly Gly Ser His Glu Ser Gly
1 5 10 15

Asp His Ser Pro Arg Ser Asn Val Arg Glu Gln Asp Arg Tyr Leu Pro
20 25 30

Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Gly
35 40 45

Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu
50 55 60

Phe Ile Ser Phe Ile Thr Ser Glu Leu Cys Gln Arg Glu Lys Arg Lys
65 70 75 80

Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Ala Thr Leu Gly Phe
85 90 95

Glu Asp Tyr Met Asp Pro Leu Lys Ile Tyr Leu Thr Arg Tyr Arg Glu
100 105 110

Met Glu Gly Asp Thr Lys Gly Ser Ala Lys Gly Gly Asp Ser Ser Ala
115 120 125

Lys Arg Asp Val Gln Pro Ser Pro Asn Ala Gln Leu Ala His Gln Gly
130 135 140

Ser Phe Ser Gln Asn Val Thr Tyr Pro Asn Ser Gln Gly Arg His Met
145 150 155 160

Met Val Pro Met Gln Gly Pro Glu
165



173
1097
DNA
Triticum aestivum

173

gcacgaggcg ccgccttctc ttctccagcg tcggatcttc ccccactcgc cgccctcacc 60

gcacctccat tcccctccac caccttccct ccctccacgc gctcctctat ataaggggga 120

gggccggatg tcggacgagg cggcgagccc cccgggcggc ggcggcggcg gaggaggcgg 180

cggcagcgac gacggcggcg gcggcggcgg cttcggcggc gtcagggagc aggacaggtt 240

cctgcccatc gccaacatca gccgcatcat gaagaaggcc atcccggcca acggcaagat 300

cgccaaggac gccaaggaga ccgtgcagga gtgcgtctcc gagttcatct ccttcatcac 360

cagcgaggcg agcgacaagt gccagaggga gaagcgcaag accatcaacg gcgacgacct 420

gctctgggcg atggccacgc tgggcttcga ggagtacatc gagcccctca aggtttatct 480

gcagaagtac agagagacgg agggtgatag taagctagct gggaagtctg gtgatgtctc 540

tgttaaaaag gatgcactgg gtcctcatgg aggagcaagt ggcacaagtg cgcaagggat 600

gggccaacaa gtagcataca atccaggaat ggtttatatg caacctcagt accataatgg 660

ggacatctca aactgaagat atggaccatc tccgagactg ctgctactct gctaggcggg 720

ttttcgtcat gtggagagca ctaagcagtt aaagaaaact cttagtaccc ccattagtct 780

cgtgttgttg ggtctgccag aactgatgct caaaggctgc ttcccagatg taaattgctt 840

tttcctgaga atagattcag ttgtggttta gcatggttgt tgttgttgtc tgtatattta 900

tgatgattag cctcgtcgtg gctgtcattc ggttccatat aatctgggta tttgggggag 960

acataactcc tccaggtgta gtttgtcctg aactagctgt atcagactct tgagaagagt 1020

tgctattagc cctccaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1080

aaaaaaaaaa aaaaaaa 1097



174
182
PRT
Triticum aestivum

174

Met Ser Asp Glu Ala Ala Ser Pro Pro Gly Gly Gly Gly Gly Gly Gly
1 5 10 15

Gly Gly Gly Ser Asp Asp Gly Gly Gly Gly Gly Gly Phe Gly Gly Val
20 25 30

Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met
35 40 45

Lys Lys Ala Ile Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu
50 55 60

Thr Val Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu
65 70 75 80

Ala Ser Asp Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp
85 90 95

Asp Leu Leu Trp Ala Met Ala Thr Leu Gly Phe Glu Glu Tyr Ile Glu
100 105 110

Pro Leu Lys Val Tyr Leu Gln Lys Tyr Arg Glu Thr Glu Gly Asp Ser
115 120 125

Lys Leu Ala Gly Lys Ser Gly Asp Val Ser Val Lys Lys Asp Ala Leu
130 135 140

Gly Pro His Gly Gly Ala Ser Gly Thr Ser Ala Gln Gly Met Gly Gln
145 150 155 160

Gln Val Ala Tyr Asn Pro Gly Met Val Tyr Met Gln Pro Gln Tyr His
165 170 175

Asn Gly Asp Ile Ser Asn
180



175
1016
DNA
Triticum aestivum

175

ctcgtgccgc aaagattgaa ttttcgtaca agtgtccttc cttccagtta acttcatgct 60

cctgcttgat caggctagag tggtttgatt gcttcttgat ttgagacaca gatcggggag 120

aggagccatg ccggagtcgg acaacgactc cggcgggccg agcaacaccg gcggggaggg 180

ggagctgtcg tcgccgcggg agcaggaccg cttcctgccc atcgccaacg tgagccgcat 240

catgaagaag gcgctcccgg ccaacgccaa gatcagcaag gacgccaagg agacggtgca 300

ggagtgcgtc tccgagttca tctccttcat caccggcgag gcctccgaca agtgccagcg 360

cgagaagcgc aagaccatca acggcgacga cctcctctgg gccatgacca ccctcggctt 420

cgaggactac gtcgaccccc tcaagcacta cctccacaag ttccgcgaga tcgagggcga 480

gagggccgcc gccacgtcga cgtcaaccgc gccgcagcac ctgcccgaca ataatgccac 540

cggttacgcc gactatggtg gcgccgctgt ccccgccccg gccccgggag gcatgatgat 600

gatggggcag cccatgtacg gctcaccgcc gccgcagcag cagcaccaac atcaggttgc 660

aatgggaggg agagcgggct ttccctatca cggaggcagc agcggtggcg gcgggtcgtc 720

ttcttcgtcg gggttcggac ggaaagaggg gtgacatctt ttcttttctt ttcgttttga 780

gctgaccaaa gtgagtgatt tcaacatatg ttcctctctt ggatgaagcc gtgacttgta 840

gcttagggaa atccattcag tacaaggagg aataattgtt cagcaaatca gttttcttct 900

ataaacagga ggaatgtata actacgagtc tacaaatcat acctgggaag ctctccatga 960

attacttgtt taacaacatg gcgagacaca ataccaatat attgatgtta aaaaaa 1016



176
208
PRT
Triticum aestivum

176

Met Pro Glu Ser Asp Asn Asp Ser Gly Gly Pro Ser Asn Thr Gly Gly
1 5 10 15

Glu Gly Glu Leu Ser Ser Pro Arg Glu Gln Asp Arg Phe Leu Pro Ile
20 25 30

Ala Asn Val Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Ala Lys
35 40 45

Ile Ser Lys Asp Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu Phe
50 55 60

Ile Ser Phe Ile Thr Gly Glu Ala Ser Asp Lys Cys Gln Arg Glu Lys
65 70 75 80

Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Thr Thr Leu
85 90 95

Gly Phe Glu Asp Tyr Val Asp Pro Leu Lys His Tyr Leu His Lys Phe
100 105 110

Arg Glu Ile Glu Gly Glu Arg Ala Ala Ala Thr Ser Thr Ser Thr Ala
115 120 125

Pro Gln His Leu Pro Asp Asn Asn Ala Thr Gly Tyr Ala Asp Tyr Gly
130 135 140

Gly Ala Ala Val Pro Ala Pro Ala Pro Gly Gly Met Met Met Met Gly
145 150 155 160

Gln Pro Met Tyr Gly Ser Pro Pro Pro Gln Gln Gln His Gln His Gln
165 170 175

Val Ala Met Gly Gly Arg Ala Gly Phe Pro Tyr His Gly Gly Ser Ser
180 185 190

Gly Gly Gly Gly Ser Ser Ser Ser Ser Gly Phe Gly Arg Lys Glu Gly
195 200 205



177
982
DNA
Triticum aestivum

177

gcacgaggca ttccccaccc ctcctcgcag cgccaaccac cgtctcctcc tcccccctcc 60

cttctctccc ctccgctcct ccccccccgc gcgcgcgttt tttataaggg tttcggggcg 120

cgggatggcc gacgacgaca gcgggagccc ccggggcggc ggcggggtca gggagcagga 180

ccgcttcctc cccatcgcca acatcagccg catcatgaag aaggccgtgc cggccaacgg 240

caagatcgcc aaggacgcca aggagaccct ccaggagtgc gtctccgagt tcatctcctt 300

cgtcaccagc gaggccagcg acaagtgcca gaaggagaag cgcaagacca tcaacgggga 360

cgatctgctc tgggccatgg ccacgctcgg attcgaggag tacgtagacc ccctcaagat 420

ctacctgcaa aagtacagag atatggaggg tgatagtaaa ttgacctcaa aatctggtga 480

aggatccgtg aagaaagata taattggtgc tcatagtggt gcgactagct caaacgccca 540

agcgatggtt cagcatggag cttacgccca agggatgggt tatatgcaac cccagtacca 600

taatggggac acctgaaact gaagatcagg caattttcgg caatgggtat tgctccatga 660

gtggttatct atctgttaag gaagccgccc caacattagg ttcatgatga tcattggctg 720

gaaactaaag cacctggaag ggtgcttaac agttggttgt gatggctgcc tccaagatgt 780

aaattgcttc cgagagaata gattcaccta ttatggttta gtgcttgttt ttatctgtac 840

attcagaata attcagccgt tggtagtttg gcaatctttt gtttcagata tttgtattag 900

gaagcataaa tatattacaa ctgggtatta acttataaaa aaaaaaaaaa aaaaaaaaaa 960

aaaaaaaaaa aaaaaaaaaa aa 982



178
163
PRT
Triticum aestivum

178

Met Ala Asp Asp Asp Ser Gly Ser Pro Arg Gly Gly Gly Gly Val Arg
1 5 10 15

Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys
20 25 30

Lys Ala Val Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr
35 40 45

Leu Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Val Thr Ser Glu Ala
50 55 60

Ser Asp Lys Cys Gln Lys Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp
65 70 75 80

Leu Leu Trp Ala Met Ala Thr Leu Gly Phe Glu Glu Tyr Val Asp Pro
85 90 95

Leu Lys Ile Tyr Leu Gln Lys Tyr Arg Asp Met Glu Gly Asp Ser Lys
100 105 110

Leu Thr Ser Lys Ser Gly Glu Gly Ser Val Lys Lys Asp Ile Ile Gly
115 120 125

Ala His Ser Gly Ala Thr Ser Ser Asn Ala Gln Ala Met Val Gln His
130 135 140

Gly Ala Tyr Ala Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His Asn
145 150 155 160

Gly Asp Thr



179
272
PRT
Arabidopsis thaliana

misc_feature

gi 11282597


179

Met Gln Ser Lys Pro Gly Arg Glu Asn Glu Glu Glu Val Asn Asn His
1 5 10 15

His Ala Val Gln Gln Pro Met Met Tyr Ala Glu Pro Trp Trp Lys Asn
20 25 30

Asn Ser Phe Gly Val Val Pro Gln Ala Arg Pro Ser Gly Ile Pro Ser
35 40 45

Asn Ser Ser Ser Leu Asp Cys Pro Asn Gly Ser Glu Ser Asn Asp Val
50 55 60

His Ser Ala Ser Glu Asp Gly Ala Leu Asn Gly Glu Asn Asp Gly Thr
65 70 75 80

Trp Lys Asp Ser Gln Ala Ala Thr Ser Ser Arg Ser Val Asp Asn His
85 90 95

Gly Met Glu Gly Asn Asp Pro Ala Leu Ser Ile Arg Asn Met His Asp
100 105 110

Gln Pro Leu Val Gln Pro Pro Glu Leu Val Gly His Tyr Ile Ala Cys
115 120 125

Val Pro Asn Pro Tyr Gln Asp Pro Tyr Tyr Gly Gly Leu Met Gly Ala
130 135 140

Tyr Gly His Gln Gln Leu Gly Phe Arg Pro Tyr Leu Gly Met Pro Arg
145 150 155 160

Glu Arg Thr Ala Leu Pro Leu Asp Met Ala Gln Glu Pro Val Tyr Val
165 170 175

Asn Ala Lys Gln Tyr Glu Gly Ile Leu Arg Arg Arg Lys Ala Arg Ala
180 185 190

Lys Ala Glu Leu Glu Arg Lys Val Ile Arg Asp Arg Lys Pro Tyr Leu
195 200 205

His Glu Ser Arg His Lys His Ala Met Arg Arg Ala Arg Ala Ser Gly
210 215 220

Gly Arg Phe Ala Lys Lys Ser Glu Val Glu Ala Gly Glu Asp Ala Gly
225 230 235 240

Gly Arg Asp Arg Glu Arg Gly Ser Ala Thr Asn Ser Ser Gly Ser Glu
245 250 255

Gln Val Glu Thr Asp Ser Asn Glu Thr Leu Asn Ser Ser Gly Ala Pro
260 265 270



180
215
PRT
Vitis riparia

misc_feature

gi 7141243


180

Met Met Pro Met Thr Met Ala Glu Tyr His Leu Ala Pro Pro Ser Gln
1 5 10 15

Leu Glu Leu Val Gly His Ser Ile Val Gln Ser Gln Phe Leu Gly Val
20 25 30

Asn Val Ala Arg Met Ala Leu Pro Ile Glu Met Ala Glu Glu Pro Val
35 40 45

Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln Ser
50 55 60

Arg Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys Val Arg Lys Pro
65 70 75 80

Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg Ala Arg Gly
85 90 95

Cys Gly Gly Arg Phe Leu Asn Thr Lys Lys Leu Asp Ser Asn Ala Ser
100 105 110

Tyr Asp Met Pro Asp Lys Gly Ser Asp Pro Asp Val Asn Leu Ser Thr
115 120 125

Arg Pro Ile Ser Ser Ser Val Ser Glu Ser Leu Pro Phe Asn Ser Ser
130 135 140

Arg Asn Glu Asp Ser Pro Thr Ser His Leu Asp Ala Arg Gly Pro Ser
145 150 155 160

Val Gln Glu Leu His Asn Arg Gln Thr Ser Ser Met Glu Met Ala Thr
165 170 175

Ser Leu Leu Ser Thr Gln Pro Gly Ile Ser Val Gly Arg Thr Tyr His
180 185 190

Ser Leu Lys Met Met Ile Gly Val Glu Arg Arg Arg Pro Arg Lys Ala
195 200 205

Ala Ser Ile Arg Glu Phe Trp
210 215



181
238
PRT
Oryza sativa

misc_feature

gi 7489565


181

Met Leu Pro Pro His Leu Thr Glu Asn Gly Thr Val Met Ile Gln Phe
1 5 10 15

Gly His Lys Met Pro Asp Tyr Glu Ser Ser Ala Thr Gln Ser Thr Ser
20 25 30

Gly Ser Pro Arg Glu Val Ser Gly Met Ser Glu Gly Ser Leu Asn Glu
35 40 45

Gln Asn Asp Gln Ser Gly Asn Leu Asp Gly Tyr Thr Lys Ser Asp Glu
50 55 60

Gly Lys Met Met Ser Ala Leu Ser Leu Gly Lys Ser Glu Thr Val Tyr
65 70 75 80

Ala His Ser Glu Pro Asp Arg Ser Gln Pro Phe Gly Ile Ser Tyr Pro
85 90 95

Tyr Ala Asp Ser Phe Tyr Gly Gly Ala Val Ala Thr Tyr Gly Thr His
100 105 110

Ala Ile Met His Pro Gln Ile Val Gly Val Met Ser Ser Ser Arg Val
115 120 125

Pro Leu Pro Ile Glu Pro Ala Thr Glu Glu Pro Ile Tyr Val Asn Ala
130 135 140

Lys Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu
145 150 155 160

Glu Ala Glu Asn Lys Leu Val Lys Asn Arg Lys Pro Tyr Leu His Glu
165 170 175

Ser Arg His Gln His Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg
180 185 190

Phe Leu Asn Thr Lys Gln Gln Pro Glu Ala Ser Asp Gly Gly Thr Pro
195 200 205

Arg Leu Val Ser Ala Asn Gly Val Val Phe Ser Lys His Glu His Ser
210 215 220

Leu Ser Ser Ser Asp Leu His His Arg Ala Lys Glu Gly Ala
225 230 235



182
197
PRT
Arabidopsis thaliana

misc_feature

gi 6634774


182

Met Thr His Thr Thr Glu Asn Thr Asn Lys Asn Arg Ala Thr Gly Arg
1 5 10 15

Asp Asn Ile Gly Ser His Glu Lys Gln Glu Gln Arg Asp Ser His Phe
20 25 30

Gln Pro Pro Ile Pro Ser Ala Arg Asn Tyr Glu Ser Ile Val Thr Ser
35 40 45

Leu Val Tyr Ser Asp Pro Gly Thr Thr Asn Ser Met Ala Pro Gly Gln
50 55 60

Tyr Pro Tyr Pro Asp Pro Tyr Tyr Arg Ser Ile Phe Ala Pro Pro Pro
65 70 75 80

Gln Pro Tyr Thr Gly Val His Leu Gln Leu Met Gly Val Gln Gln Gln
85 90 95

Gly Val Pro Leu Pro Ser Asp Ala Val Glu Glu Pro Val Phe Val Asn
100 105 110

Ala Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln Ser Arg Ala Arg
115 120 125

Leu Glu Ser Gln Asn Lys Val Ile Lys Ser Arg Lys Pro Tyr Leu His
130 135 140

Glu Ser Arg His Leu His Ala Ile Arg Arg Pro Arg Gly Cys Gly Gly
145 150 155 160

Arg Phe Leu Asn Ala Lys Lys Glu Asp Glu His His Glu Asp Ser Ser
165 170 175

His Glu Glu Lys Ser Asn Leu Ser Ala Gly Lys Ser Ala Met Ala Ala
180 185 190

Ser Ser Gly Thr Ser
195



183
298
PRT
Arabidopsis thaliana

misc_feature

gi 9293997


183

Met His Ser Lys Ser Asp Ser Gly Gly Asn Lys Val Asp Ser Glu Val
1 5 10 15

His Gly Thr Val Ser Ser Ser Ile Asn Ser Leu Asn Pro Trp His Arg
20 25 30

Ala Ala Ala Ala Cys Asn Ala Asn Ser Ser Val Glu Ala Gly Asp Lys
35 40 45

Ser Ser Lys Ser Ile Ala Leu Ala Leu Glu Ser Asn Gly Ser Lys Ser
50 55 60

Pro Ser Asn Arg Asp Asn Thr Val Asn Lys Glu Ser Gln Val Thr Thr
65 70 75 80

Ser Pro Gln Ser Ala Gly Asp Tyr Ser Asp Lys Asn Gln Glu Ser Leu
85 90 95

His His Gly Ile Thr Gln Pro Pro Pro His Pro Gln Leu Val Gly His
100 105 110

Thr Val Gly Trp Ala Ser Ser Asn Pro Tyr Gln Asp Pro Tyr Tyr Ala
115 120 125

Gly Val Met Gly Ala Tyr Gly His His Pro Leu Gly Phe Val Pro Tyr
130 135 140

Gly Gly Met Pro His Ser Arg Met Pro Leu Pro Pro Glu Met Ala Gln
145 150 155 160

Glu Pro Val Phe Val Asn Ala Lys Gln Tyr Gln Ala Ile Leu Arg Arg
165 170 175

Arg Gln Ala Arg Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys Ser
180 185 190

Arg Lys Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg
195 200 205

Pro Arg Gly Thr Gly Gly Arg Phe Ala Lys Lys Thr Asn Thr Glu Ala
210 215 220

Ser Lys Arg Lys Ala Glu Glu Lys Ser Asn Gly His Val Thr Gln Ser
225 230 235 240

Pro Ser Ser Ser Asn Ser Asp Gln Gly Glu Ala Trp Asn Gly Asp Tyr
245 250 255

Arg Thr Pro Gln Gly Asp Glu Met Gln Ser Ser Ala Tyr Lys Arg Arg
260 265 270

Glu Glu Gly Glu Cys Ser Gly Gln Gln Trp Asn Ser Leu Ser Ser Asn
275 280 285

His Pro Ser Gln Ala Arg Leu Ala Ile Lys
290 295



184
340
PRT
Arabidopsis thaliana

misc_feature

gi 5903072


184

Met Met His Gln Met Leu Asn Lys Lys Asp Ser Ala Thr His Ser Thr
1 5 10 15

Leu Pro Tyr Leu Asn Thr Ser Ile Ser Trp Gly Val Val Pro Thr Asp
20 25 30

Ser Val Ala Asn Arg Arg Gly Ser Ala Glu Ser Leu Ser Leu Lys Val
35 40 45

Asp Ser Arg Pro Gly His Ile Gln Thr Thr Lys Gln Ile Ser Phe Gln
50 55 60

Asp Gln Asp Ser Ser Ser Thr Gln Ser Thr Gly Gln Ser Tyr Thr Glu
65 70 75 80

Val Ala Ser Ser Gly Asp Asp Asn Pro Ser Arg Gln Ile Ser Phe Ser
85 90 95

Ala Lys Ser Gly Ser Glu Ile Thr Gln Arg Lys Gly Phe Ala Ser Asn
100 105 110

Pro Lys Gln Gly Ser Met Thr Gly Phe Pro Asn Ile His Phe Ala Pro
115 120 125

Ala Gln Ala Asn Phe Ser Phe His Tyr Ala Asp Pro His Tyr Gly Gly
130 135 140

Leu Leu Ala Ala Thr Tyr Leu Pro Gln Ala Pro Thr Cys Asn Pro Gln
145 150 155 160

Met Val Ser Met Ile Pro Gly Arg Val Pro Leu Pro Ala Glu Leu Thr
165 170 175

Glu Thr Asp Pro Val Phe Val Asn Ala Lys Gln Tyr His Ala Ile Met
180 185 190

Arg Arg Arg Gln Gln Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Ile
195 200 205

Arg Ala Arg Lys Pro Tyr Leu His Glu Ser Arg His Val His Ala Leu
210 215 220

Lys Arg Pro Arg Gly Ser Gly Gly Arg Phe Leu Asn Thr Lys Lys Leu
225 230 235 240

Leu Gln Glu Ser Glu Gln Ala Ala Ala Arg Glu Gln Glu Gln Asp Lys
245 250 255

Leu Gly Gln Gln Val Asn Arg Lys Thr Asn Met Ser Arg Phe Glu Ala
260 265 270

His Met Leu Gln Asn Asn Lys Asp Arg Ser Ser Thr Thr Ser Gly Ser
275 280 285

Asp Ile Thr Ser Val Ser Asp Gly Ala Asp Ile Phe Gly His Thr Glu
290 295 300

Phe Gln Phe Ser Gly Phe Pro Thr Pro Ile Asn Arg Ala Met Leu Val
305 310 315 320

His Gly Gln Ser Asn Asp Met His Gly Gly Gly Asp Met His His Phe
325 330 335

Ser Val His Ile
340



185
355
PRT
Arabidopsis thaliana

misc_feature

gi 8778470


185

Met Asp Lys Lys Val Ser Phe Thr Ser Ser Val Ala His Ser Thr Pro
1 5 10 15

Pro Tyr Leu Ser Thr Ser Ile Ser Trp Gly Leu Pro Thr Lys Ser Asn
20 25 30

Gly Val Thr Glu Ser Leu Ser Leu Lys Val Val Asp Ala Arg Pro Glu
35 40 45

Arg Leu Ile Asn Thr Lys Asn Ile Ser Phe Gln Asp Gln Asp Ser Ser
50 55 60

Ser Thr Leu Ser Ser Ala Gln Ser Ser Asn Asp Val Thr Ser Ser Gly
65 70 75 80

Asp Asp Asn Pro Ser Arg Gln Ile Ser Phe Leu Ala His Ser Asp Val
85 90 95

Cys Lys Gly Phe Glu Glu Thr Gln Arg Lys Arg Phe Ala Ile Lys Ser
100 105 110

Gly Ser Ser Thr Ala Gly Ile Ala Asp Ile His Ser Ser Pro Ser Lys
115 120 125

Val Pro Val Tyr Leu Leu Arg Val Thr Ile Ser Ser Thr Cys Asp Cys
130 135 140

Leu Leu Thr Ser Cys Val Ile Leu Trp Phe Gln Ala Asn Phe Ser Phe
145 150 155 160

His Tyr Ala Asp Pro His Phe Gly Gly Leu Met Pro Ala Ala Tyr Leu
165 170 175

Pro Gln Ala Thr Ile Trp Asn Pro Gln Met Thr Arg Val Pro Leu Pro
180 185 190

Phe Asp Leu Ile Glu Asn Glu Pro Val Phe Val Asn Ala Lys Gln Phe
195 200 205

His Ala Ile Met Arg Arg Arg Gln Gln Arg Ala Lys Leu Glu Ala Gln
210 215 220

Asn Lys Leu Ile Lys Ala Arg Lys Pro Tyr Leu His Glu Ser Arg His
225 230 235 240

Val His Ala Leu Lys Arg Pro Arg Gly Ser Gly Gly Arg Phe Leu Asn
245 250 255

Thr Lys Lys Leu Gln Glu Ser Thr Asp Pro Lys Gln Asp Met Pro Ile
260 265 270

Gln Gln Gln His Ala Thr Gly Asn Met Ser Arg Phe Val Leu Tyr Gln
275 280 285

Leu Gln Asn Ser Asn Asp Cys Asp Cys Ser Thr Thr Ser Arg Ser Asp
290 295 300

Ile Thr Ser Ala Ser Asp Ser Val Asn Leu Phe Gly His Ser Glu Phe
305 310 315 320

Leu Ile Ser Asp Cys Pro Ser Gln Thr Asn Pro Thr Met Tyr Val His
325 330 335

Gly Gln Ser Asn Asp Met His Gly Gly Arg Asn Thr His His Phe Ser
340 345 350

Val His Ile
355



186
271
PRT
Arabidopsis thaliana

misc_feature

gi 2398521


186

Met Gln Ser Lys Pro Gly Arg Glu Asn Glu Glu Glu Val Asn Asn His
1 5 10 15

His Ala Val Gln Gln Pro Met Met Tyr Ala Glu Pro Trp Trp Lys Asn
20 25 30

Asn Ser Phe Gly Val Val Pro Gln Ala Arg Pro Ser Gly Ile Pro Ser
35 40 45

Asn Ser Ser Ser Leu Asp Cys Pro Asn Gly Ser Glu Ser Asn Asp Val
50 55 60

His Ser Ala Ser Glu Asp Gly Ala Leu Asn Gly Glu Asn Asp Gly Thr
65 70 75 80

Trp Lys Asp Ser Gln Ala Ala Thr Ser Ser Arg Ser Asp Asn His Gly
85 90 95

Met Glu Gly Asn Asp Pro Ala Leu Ser Ile Arg Asn Met His Asp Gln
100 105 110

Pro Leu Val Gln Pro Pro Glu Leu Val Gly His Tyr Ile Ala Cys Val
115 120 125

Pro Asn Pro Tyr Gln Asp Pro Tyr Tyr Gly Gly Leu Met Gly Ala Tyr
130 135 140

Gly His Gln Gln Leu Gly Phe Arg Pro Tyr Leu Gly Met Pro Arg Glu
145 150 155 160

Arg Thr Ala Leu Pro Leu Asp Met Ala Gln Glu Pro Val Tyr Val Asn
165 170 175

Ala Lys Gln Tyr Glu Gly Ile Leu Arg Arg Arg Lys Ala Arg Ala Lys
180 185 190

Ala Glu Leu Glu Arg Lys Val Ile Arg Asp Arg Lys Pro Tyr Leu His
195 200 205

Glu Ser Arg His Lys His Ala Met Arg Arg Ala Arg Ala Ser Gly Gly
210 215 220

Arg Phe Ala Lys Lys Ser Glu Val Glu Ala Gly Glu Asp Ala Gly Gly
225 230 235 240

Arg Asp Arg Glu Arg Gly Ser Ala Thr Asn Ser Ser Gly Ser Glu Gln
245 250 255

Val Glu Thr Asp Ser Asn Glu Thr Leu Asn Ser Ser Gly Ala Pro
260 265 270



187
315
PRT
Brassica napus

misc_feature

gi 1586551


187

Met Ile Ser Leu Thr Val Thr Thr Pro Ser Leu Arg Met Glu Thr Glu
1 5 10 15

Asp Met His Ser Lys Ser Glu Ser Gly Asn Gln Ile Val Ser Glu Ala
20 25 30

His His His Thr Ser Ser Thr Ser Ile Asn Ser Leu Asn Pro Trp Leu
35 40 45

Arg Ala Ala Ala Ser Cys Asn Ala Asn Ser Ser Val Glu Glu Ala Gly
50 55 60

Asp Lys Ser Ile Ala Leu Glu Asn Gln Thr Asn Leu Glu Ser Ser Asn
65 70 75 80

Gly Ser Lys Ser Pro Ser Asn Arg Asp Glu Asn Gly Asn Lys Glu Ser
85 90 95

Gln Val Thr Ala Ser Pro Gln Gln Ser Ala Ala Asp Tyr Ser Glu Lys
100 105 110

Ser Gln Glu Leu Val His Pro Gly Ser Thr Pro Pro Pro His Pro Gln
115 120 125

Leu Val Ser His Thr Val Gly Trp Ala Ser Ser Asn Pro Tyr Gln Asp
130 135 140

Ser Tyr Tyr Ala Gly Met Met Gly Ala Tyr Pro Leu Thr Tyr Val Pro
145 150 155 160

His Gly Gly Met Pro His Ser Arg Met Gln Leu Pro Pro Glu Met Ala
165 170 175

Gln Glu Pro Val Tyr Val Asn Ala Lys Gln Tyr Gln Ala Ile Met Arg
180 185 190

Arg Arg Gln Ala Arg Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys
195 200 205

Ser Arg Lys Arg Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg
210 215 220

Arg Pro Arg Gly Thr Gly Gly Arg Phe Ala Lys Lys Thr Asn Thr Glu
225 230 235 240

Ala Ser Gln Gln Lys Asp Gly Glu Lys Arg Asn Ala Cys Ala Thr Gln
245 250 255

Ser Pro Thr Ser Ser His Ser Asp Gln His Glu Gly Cys Ser Asp Glu
260 265 270

Tyr Arg Thr Asn Gln Ser Asp Glu Met Gln Ser Ser Ala Tyr Lys Ile
275 280 285

Arg Glu Glu Ala Asp Cys Ser Gly Gln Gln Trp Asn Asn Ile Ser Ser
290 295 300

Asn His Pro Ser Gln Pro Leu Leu Ala Ile Lys
305 310 315



188
295
PRT
Arabidopsis thaliana

misc_feature

gi 6714441


188

Met Ala Met Gln Thr Val Arg Glu Gly Leu Phe Ser Ala Pro Gln Thr
1 5 10 15

Ser Trp Trp Thr Ala Phe Gly Ser Gln Pro Leu Ala Pro Glu Ser Leu
20 25 30

Ala Gly Asp Ser Asp Ser Phe Ala Gly Val Lys Val Gly Ser Val Gly
35 40 45

Glu Thr Gly Gln Arg Val Asp Lys Gln Ser Asn Ser Ala Thr His Leu
50 55 60

Ala Phe Ser Leu Gly Asp Val Lys Ser Pro Arg Leu Val Pro Lys Pro
65 70 75 80

His Gly Ala Thr Phe Ser Met Gln Ser Pro Cys Leu Glu Leu Gly Phe
85 90 95

Ser Gln Pro Pro Ile Tyr Thr Lys Tyr Pro Tyr Gly Glu Gln Gln Tyr
100 105 110

Tyr Gly Val Val Ser Ala Tyr Gly Ser Gln Ser Arg Val Met Leu Pro
115 120 125

Leu Asn Met Glu Thr Glu Asp Ser Thr Ile Tyr Val Asn Ser Lys Gln
130 135 140

Tyr His Gly Ile Ile Arg Arg Arg Gln Ser Arg Ala Lys Ala Ala Ala
145 150 155 160

Val Leu Asp Gln Lys Lys Leu Ser Ser Arg Cys Arg Lys Pro Tyr Met
165 170 175

His His Ser Arg His Leu His Ala Leu Arg Arg Pro Arg Gly Ser Gly
180 185 190

Gly Arg Phe Leu Asn Thr Lys Ser Gln Asn Leu Glu Asn Ser Gly Thr
195 200 205

Asn Ala Lys Lys Gly Asp Gly Ser Met Gln Ile Gln Ser Gln Pro Lys
210 215 220

Pro Gln Gln Ser Asn Ser Gln Asn Ser Glu Val Val His Pro Glu Asn
225 230 235 240

Gly Thr Met Asn Leu Ser Asn Gly Leu Asn Val Ser Gly Ser Glu Val
245 250 255

Thr Ser Met Asn Tyr Phe Leu Ser Ser Pro Val His Ser Leu Gly Gly
260 265 270

Met Val Met Pro Ser Lys Trp Ile Ala Ala Ala Ala Ala Met Asp Asn
275 280 285

Gly Cys Cys Asn Phe Lys Thr
290 295



189
205
PRT
Oryza sativa

misc_feature

gi 5257260


189

Met Glu Pro Lys Ser Thr Thr Pro Pro Pro Pro Pro Pro Pro Pro Val
1 5 10 15

Leu Gly Ala Pro Val Pro Tyr Pro Pro Ala Gly Ala Tyr Pro Pro Pro
20 25 30

Val Gly Pro Tyr Ala His Ala Pro Pro Leu Tyr Ala Pro Pro Pro Pro
35 40 45

Ala Ala Ala Ala Ala Ser Ala Ala Ala Thr Ala Ala Ser Gln Gln Ala
50 55 60

Ala Ala Ala Gln Leu Gln Asn Phe Trp Ala Glu Gln Tyr Arg Glu Ile
65 70 75 80

Glu His Thr Thr Asp Phe Lys Asn His Asn Leu Pro Leu Ala Arg Ile
85 90 95

Lys Lys Ile Met Lys Ala Asp Glu Asp Val Arg Met Ile Ala Ala Glu
100 105 110

Ala Pro Val Val Phe Ala Arg Ala Cys Glu Met Phe Ile Leu Glu Leu
115 120 125

Thr His Arg Gly Trp Ala His Ala Glu Glu Asn Lys Arg Arg Thr Leu
130 135 140

Gln Lys Ser Asp Ile Ala Ala Ala Ile Ala Arg Thr Glu Val Phe Asp
145 150 155 160

Phe Leu Val Asp Ile Val Pro Arg Asp Glu Ala Lys Asp Ala Glu Ala
165 170 175

Ala Ala Ala Val Ala Ala Gly Ile Pro His Pro Ala Ala Gly Leu Pro
180 185 190

Ala Thr Asp Pro Met Ala Tyr Tyr Tyr Val Gln Pro Gln
195 200 205



190
234
PRT
Arabidopsis thaliana

misc_feature

gi 6523090


190

Met Asp Thr Asn Asn Gln Gln Pro Pro Pro Ser Ala Ala Gly Ile Pro
1 5 10 15

Pro Pro Pro Pro Gly Thr Thr Ile Ser Ala Ala Gly Gly Gly Ala Ser
20 25 30

Tyr His His Leu Leu Gln Gln Gln Gln Gln Gln Leu Gln Leu Phe Trp
35 40 45

Thr Tyr Gln Arg Gln Glu Ile Glu Gln Val Asn Asp Phe Lys Asn His
50 55 60

Gln Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu Asp
65 70 75 80

Val Arg Met Ile Ser Ala Glu Ala Pro Ile Leu Phe Ala Lys Ala Cys
85 90 95

Glu Leu Phe Ile Leu Glu Leu Thr Ile Arg Ser Trp Leu His Ala Glu
100 105 110

Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala Ala Ile
115 120 125

Thr Arg Thr Asp Ile Phe Asp Phe Leu Val Asp Ile Val Pro Arg Asp
130 135 140

Glu Ile Lys Asp Glu Ala Ala Val Leu Gly Gly Gly Met Val Val Ala
145 150 155 160

Pro Thr Ala Ser Gly Val Pro Tyr Tyr Tyr Pro Pro Met Gly Gln Pro
165 170 175

Ala Gly Pro Gly Gly Met Met Ile Gly Arg Pro Ala Met Asp Pro Asn
180 185 190

Gly Val Tyr Val Gln Pro Pro Ser Gln Ala Trp Gln Ser Val Trp Gln
195 200 205

Thr Ser Thr Gly Thr Gly Asp Asp Val Ser Tyr Gly Ser Gly Gly Ser
210 215 220

Ser Gly Gln Gly Asn Leu Asp Gly Gln Gly
225 230



191
217
PRT
Arabidopsis thaliana

misc_feature

gi 3776575


191

Met Asp Gln Gln Gly Gln Ser Ser Ala Met Asn Tyr Gly Ser Asn Pro
1 5 10 15

Tyr Gln Thr Asn Ala Met Thr Thr Thr Pro Thr Gly Ser Asp His Pro
20 25 30

Ala Tyr His Gln Ile His Gln Gln Gln Gln Gln Gln Leu Thr Gln Gln
35 40 45

Leu Gln Ser Phe Trp Glu Thr Gln Phe Lys Glu Ile Glu Lys Thr Thr
50 55 60

Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile Met
65 70 75 80

Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val Val
85 90 95

Phe Ala Arg Ala Cys Glu Met Phe Ile Leu Glu Leu Thr Leu Arg Ser
100 105 110

Trp Asn His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp
115 120 125

Ile Ala Ala Ala Val Thr Arg Thr Asp Ile Phe Asp Phe Leu Val Asp
130 135 140

Ile Val Pro Arg Glu Asp Leu Arg Asp Glu Val Leu Gly Gly Val Gly
145 150 155 160

Ala Glu Ala Ala Thr Ala Ala Gly Tyr Pro Tyr Gly Tyr Leu Pro Pro
165 170 175

Gly Thr Ala Pro Ile Gly Asn Pro Gly Met Val Met Gly Asn Pro Gly
180 185 190

Ala Tyr Pro Pro Asn Pro Tyr Met Gly Gln Pro Met Trp Gln Gln Pro
195 200 205

Gly Pro Glu Gln Gln Asp Pro Asp Asn
210 215



192
231
PRT
Arabidopsis thaliana

misc_feature

gi 6289057


192

Met Asp Gln Gln Asp His Gly Gln Ser Gly Ala Met Asn Tyr Gly Thr
1 5 10 15

Asn Pro Tyr Gln Thr Asn Pro Met Ser Thr Thr Ala Ala Thr Val Ala
20 25 30

Gly Gly Ala Ala Gln Pro Gly Gln Leu Ala Phe His Gln Ile His Gln
35 40 45

Gln Gln Gln Gln Gln Gln Leu Ala Gln Gln Leu Gln Ala Phe Trp Glu
50 55 60

Asn Gln Phe Lys Glu Ile Glu Lys Thr Thr Asp Phe Lys Lys His Ser
65 70 75 80

Leu Pro Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu Asp Val
85 90 95

Arg Met Ile Ser Ala Glu Ala Pro Val Val Phe Ala Arg Ala Cys Glu
100 105 110

Met Phe Ile Leu Glu Leu Thr Leu Arg Ser Trp Asn His Thr Glu Glu
115 120 125

Asn Lys Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala Ala Val Thr
130 135 140

Arg Thr Asp Ile Phe Asp Phe Leu Val Asp Ile Val Pro Arg Glu Asp
145 150 155 160

Leu Arg Asp Glu Val Leu Gly Ser Ile Pro Arg Gly Thr Val Pro Glu
165 170 175

Ala Ala Ala Ala Gly Tyr Pro Tyr Gly Tyr Leu Pro Ala Gly Thr Ala
180 185 190

Pro Ile Gly Asn Pro Gly Met Val Met Gly Asn Pro Gly Gly Ala Tyr
195 200 205

Pro Pro Asn Pro Tyr Met Gly Gln Pro Met Trp Gln Gln Gln Ala Pro
210 215 220

Asp Gln Pro Asp Gln Glu Asn
225 230



193
137
PRT
Arabidopsis thaliana

misc_feature

gi 6056368


193

Met Gln Glu Ile Glu His Thr Thr Asp Phe Lys Asn His Thr Leu Pro
1 5 10 15

Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu Asp Val Arg Met
20 25 30

Ile Ser Ala Glu Ala Pro Val Ile Phe Ala Lys Ala Cys Glu Met Phe
35 40 45

Ile Leu Glu Leu Thr Leu Arg Ala Trp Ile His Thr Glu Glu Asn Lys
50 55 60

Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala Ala Ile Ser Arg Thr
65 70 75 80

Asp Val Phe Asp Phe Leu Val Asp Ile Ile Pro Arg Asp Glu Leu Lys
85 90 95

Glu Glu Gly Leu Gly Val Thr Lys Gly Thr Ile Pro Ser Val Val Gly
100 105 110

Ser Pro Pro Tyr Tyr Tyr Leu Gln Gln Gln Gly Met Met Gln His Trp
115 120 125

Pro Gln Glu Gln His Pro Asp Glu Ser
130 135



194
250
PRT
Arabidopsis thaliana

misc_feature

gi 9758288


194

Met Asp Asn Asn Asn Asn Asn Asn Asn Gln Gln Pro Pro Pro Thr Ser
1 5 10 15

Val Tyr Pro Pro Gly Ser Ala Val Thr Thr Val Ile Pro Pro Pro Pro
20 25 30

Ser Gly Ser Ala Ser Ile Val Thr Gly Gly Gly Ala Thr Tyr His His
35 40 45

Leu Leu Gln Gln Gln Gln Gln Gln Leu Gln Met Phe Trp Thr Tyr Gln
50 55 60

Arg Gln Glu Ile Glu Gln Val Asn Asp Phe Lys Asn His Gln Leu Pro
65 70 75 80

Leu Ala Arg Ile Lys Lys Ile Met Lys Ala Asp Glu Asp Val Arg Met
85 90 95

Ile Ser Ala Glu Ala Pro Ile Leu Phe Ala Lys Ala Cys Glu Leu Phe
100 105 110

Ile Leu Glu Leu Thr Ile Arg Ser Trp Leu His Ala Glu Glu Asn Lys
115 120 125

Arg Arg Thr Leu Gln Lys Asn Asp Ile Ala Ala Ala Ile Thr Arg Thr
130 135 140

Asp Ile Phe Asp Phe Leu Val Asp Ile Val Pro Arg Glu Glu Ile Lys
145 150 155 160

Glu Glu Glu Asp Ala Ala Ser Ala Leu Gly Gly Gly Gly Met Val Ala
165 170 175

Pro Ala Ala Ser Gly Val Pro Tyr Tyr Tyr Pro Pro Met Gly Gln Pro
180 185 190

Ala Val Pro Gly Gly Met Met Ile Gly Arg Pro Ala Met Asp Pro Ser
195 200 205

Gly Val Tyr Ala Gln Pro Pro Ser Gln Ala Trp Gln Ser Val Trp Gln
210 215 220

Asn Ser Ala Gly Gly Gly Asp Asp Val Ser Tyr Gly Ser Gly Gly Ser
225 230 235 240

Ser Gly His Gly Asn Leu Asp Ser Gln Gly
245 250



195
208
PRT
Arabidopsis thaliana

misc_feature

gi 6552738


195

Met Thr Ser Ser Val Val Val Ala Gly Ala Gly Asp Lys Asn Asn Gly
1 5 10 15

Ile Val Val Gln Gln Gln Pro Pro Cys Val Ala Arg Glu Gln Asp Gln
20 25 30

Tyr Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg Lys Thr Leu Pro
35 40 45

Ser His Ala Lys Ile Ser Asp Asp Ala Lys Glu Thr Ile Gln Glu Cys
50 55 60

Val Ser Glu Tyr Ile Ser Phe Val Thr Gly Glu Ala Asn Glu Arg Cys
65 70 75 80

Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu Asp Ile Leu Trp Ala
85 90 95

Met Ser Lys Leu Gly Phe Asp Asn Tyr Val Asp Pro Leu Thr Val Phe
100 105 110

Ile Asn Arg Tyr Arg Glu Ile Glu Thr Asp Arg Gly Ser Ala Leu Arg
115 120 125

Gly Glu Pro Pro Ser Leu Arg Gln Thr Tyr Gly Gly Asn Gly Ile Gly
130 135 140

Phe His Gly Pro Ser His Gly Leu Pro Pro Pro Gly Pro Tyr Gly Tyr
145 150 155 160

Gly Met Leu Asp Gln Ser Met Val Met Gly Gly Gly Arg Tyr Tyr Gln
165 170 175

Asn Gly Ser Ser Gly Gln Asp Glu Ser Ser Val Gly Gly Gly Ser Ser
180 185 190

Ser Ser Ile Asn Gly Met Pro Ala Phe Asp His Tyr Gly Gln Tyr Lys
195 200 205



196
205
PRT
Arabidopsis thaliana

misc_feature

gi 9758795


196

Met Ala Glu Gly Ser Met Arg Pro Pro Glu Phe Asn Gln Pro Asn Lys
1 5 10 15

Thr Ser Asn Gly Gly Glu Glu Glu Cys Thr Val Arg Glu Gln Asp Arg
20 25 30

Phe Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg Arg Ile Leu Pro
35 40 45

Ala His Ala Lys Ile Ser Asp Asp Ser Lys Glu Thr Ile Gln Glu Cys
50 55 60

Val Ser Glu Tyr Ile Ser Phe Ile Thr Gly Glu Ala Asn Glu Arg Cys
65 70 75 80

Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu Asp Val Leu Trp Ala
85 90 95

Met Ser Lys Leu Gly Phe Asp Asp Tyr Ile Glu Pro Leu Thr Leu Tyr
100 105 110

Leu His Arg Tyr Arg Glu Leu Glu Gly Glu Arg Gly Val Ser Cys Ser
115 120 125

Ala Gly Ser Val Ser Met Thr Asn Gly Leu Val Val Lys Arg Pro Asn
130 135 140

Gly Thr Met Thr Glu Tyr Gly Ala Tyr Gly Pro Val Pro Gly Ile His
145 150 155 160

Met Ala Gln Tyr His Tyr Arg His Gln Asn Gly Phe Val Phe Ser Gly
165 170 175

Asn Glu Pro Asn Ser Lys Met Ser Gly Ser Ser Ser Gly Ala Ser Gly
180 185 190

Ala Arg Val Glu Val Phe Pro Thr Gln Gln His Lys Tyr
195 200 205



197
178
PRT
Zea mays

misc_feature

gi 22380


197

Met Ala Glu Ala Pro Ala Ser Pro Gly Gly Gly Gly Gly Ser His Glu
1 5 10 15

Ser Gly Ser Pro Arg Gly Gly Gly Gly Gly Gly Ser Val Arg Glu Gln
20 25 30

Asp Arg Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Lys Ala
35 40 45

Ile Pro Ala Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Val Gln
50 55 60

Glu Cys Val Ser Glu Phe Ile Ser Phe Ile Thr Ser Glu Ala Ser Asp
65 70 75 80

Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu
85 90 95

Trp Ala Met Ala Thr Leu Gly Phe Glu Asp Tyr Ile Glu Pro Leu Lys
100 105 110

Val Tyr Leu Gln Lys Tyr Arg Glu Met Glu Gly Asp Ser Lys Leu Thr
115 120 125

Ala Lys Ser Ser Asp Gly Ser Ile Lys Lys Asp Ala Leu Gly His Val
130 135 140

Gly Ala Ser Ser Ser Ala Ala Glu Gly Met Gly Gln Gln Gly Ala Tyr
145 150 155 160

Asn Gln Gly Met Gly Tyr Met Gln Pro Gln Tyr His Asn Gly Asp Ile
165 170 175

Ser Asn



198
228
PRT
Arabidopsis thaliana

misc_feature

gi 6729485


198

Met Ala Glu Ser Gln Thr Gly Gly Gly Gly Gly Gly Ser His Glu Ser
1 5 10 15

Gly Gly Asp Gln Ser Pro Arg Ser Leu Asn Val Arg Glu Gln Asp Arg
20 25 30

Phe Leu Pro Ile Ala Asn Ile Ser Arg Ile Met Lys Arg Gly Leu Pro
35 40 45

Leu Asn Gly Lys Ile Ala Lys Asp Ala Lys Glu Thr Met Gln Glu Cys
50 55 60

Val Ser Glu Phe Ile Ser Phe Val Thr Ser Glu Ala Ser Asp Lys Cys
65 70 75 80

Gln Arg Glu Lys Arg Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala
85 90 95

Met Ala Thr Leu Gly Phe Glu Asp Tyr Ile Asp Pro Leu Lys Val Tyr
100 105 110

Leu Met Arg Tyr Arg Glu Met Glu Gly Asp Thr Lys Gly Ser Gly Lys
115 120 125

Gly Gly Glu Ser Ser Ala Lys Arg Asp Gly Gln Pro Ser Gln Val Ser
130 135 140

Gln Phe Ser Gln Val Pro Gln Gln Gly Ser Phe Ser Gln Gly Pro Tyr
145 150 155 160

Gly Asn Ser Gln Ser Leu Arg Phe Gly Asn Ser Ile Glu His Leu Glu
165 170 175

Val Leu Met Ser Ser Thr Arg Thr Leu Phe Ile Thr Ile Phe Arg Asp
180 185 190

Ser Thr Met Pro Val Val Ser Glu Asn Leu Ser Asp Pro Leu Ser Ile
195 200 205

Asp Met Asp Cys Glu Ala Ile Tyr His His Phe Ile Gly Leu Leu Ile
210 215 220

Leu Ser Cys Lys
225



199
161
PRT
Arabidopsis thaliana

misc_feature

gi 2244810


199

Met Ala Asp Ser Asp Asn Asp Ser Gly Gly His Lys Asp Gly Gly Asn
1 5 10 15

Ala Ser Thr Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala Asn Val Ser
20 25 30

Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Ala Lys Ile Ser Lys Asp
35 40 45

Ala Lys Glu Thr Val Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Ile
50 55 60

Thr Gly Glu Ala Ser Asp Lys Cys Gln Arg Glu Lys Arg Lys Thr Ile
65 70 75 80

Asn Gly Asp Asp Leu Leu Trp Ala Met Thr Thr Leu Gly Phe Glu Asp
85 90 95

Tyr Val Glu Pro Leu Lys Val Tyr Leu Gln Lys Tyr Arg Glu Val Glu
100 105 110

Gly Glu Lys Thr Thr Thr Ala Gly Arg Gln Gly Asp Lys Glu Gly Gly
115 120 125

Gly Gly Gly Gly Gly Ala Gly Ser Gly Ser Gly Gly Ala Pro Met Tyr
130 135 140

Gly Gly Gly Met Val Thr Thr Met Gly His Gln Phe Ser His His Phe
145 150 155 160

Ser



200
187
PRT
Arabidopsis thaliana

misc_feature

gi 2398529


200

Arg Asp Arg Asp Ser Gly Gly Gly Gln Asn Gly Asn Asn Gln Asn Gly
1 5 10 15

Gln Ser Ser Leu Ser Pro Arg Glu Gln Asp Arg Phe Leu Pro Ile Ala
20 25 30

Asn Val Ser Arg Ile Met Lys Lys Ala Leu Pro Ala Asn Ala Lys Ile
35 40 45

Ser Lys Asp Ala Lys Glu Thr Met Gln Glu Cys Val Ser Glu Phe Ile
50 55 60

Ser Phe Val Thr Gly Glu Ala Ser Asp Lys Cys Gln Lys Glu Lys Arg
65 70 75 80

Lys Thr Ile Asn Gly Asp Asp Leu Leu Trp Ala Met Thr Thr Leu Gly
85 90 95

Phe Glu Asp Tyr Val Glu Pro Leu Lys Val Tyr Leu Gln Arg Phe Arg
100 105 110

Glu Ile Glu Gly Glu Arg Thr Gly Leu Gly Arg Pro Gln Thr Gly Gly
115 120 125

Glu Val Gly Glu His Gln Arg Asp Ala Val Gly Asp Gly Gly Gly Phe
130 135 140

Tyr Gly Gly Gly Gly Gly Met Gln Tyr His Gln His His Gln Phe Leu
145 150 155 160

His Gln Gln Asn His Met Tyr Gly Ala Thr Gly Gly Gly Ser Asp Ser
165 170 175

Gly Gly Gly Ala Ala Ser Gly Arg Thr Arg Thr
180 185



201
160
PRT
Arabidopsis thaliana

misc_feature

gi 3738293


201

Met Ala Gly Asn Tyr His Ser Phe Gln Asn Pro Ile Pro Arg Tyr Gln
1 5 10 15

Asn Tyr Asn Phe Gly Ser Ser Ser Ser Asn His Gln His Glu His Asp
20 25 30

Gly Leu Val Val Val Val Glu Asp Gln Gln Gln Glu Glu Ser Met Met
35 40 45

Val Lys Glu Gln Asp Arg Leu Leu Pro Ile Ala Asn Val Gly Arg Ile
50 55 60

Met Lys Asn Ile Leu Pro Ala Asn Ala Lys Val Ser Lys Glu Ala Lys
65 70 75 80

Glu Thr Met Gln Glu Cys Val Ser Glu Phe Ile Ser Phe Val Thr Gly
85 90 95

Glu Ala Ser Asp Lys Cys His Lys Glu Lys Arg Lys Thr Val Asn Gly
100 105 110

Asp Asp Ile Cys Trp Ala Met Ala Asn Leu Gly Phe Asp Asp Tyr Ala
115 120 125

Ala Gln Leu Lys Lys Tyr Leu His Arg Tyr Arg Val Leu Glu Gly Glu
130 135 140

Lys Pro Asn His His Gly Lys Gly Gly Pro Lys Ser Ser Pro Asp Asn
145 150 155 160



202
308
PRT
Arabidopsis thaliana

misc_feature

gi 4587559


202

Met Gln Val Phe Gln Arg Lys Glu Asp Ser Ser Trp Gly Asn Ser Met
1 5 10 15

Pro Thr Thr Asn Ser Asn Ile Gln Gly Ser Glu Ser Phe Ser Leu Thr
20 25 30

Lys Asp Met Ile Met Ser Thr Thr Gln Leu Pro Ala Met Lys His Ser
35 40 45

Gly Leu Gln Leu Gln Asn Gln Asp Ser Thr Ser Ser Gln Ser Thr Glu
50 55 60

Glu Glu Ser Gly Gly Gly Glu Val Ala Ser Phe Gly Glu Tyr Lys Arg
65 70 75 80

Tyr Gly Cys Ser Ile Val Asn Asn Asn Leu Ser Gly Tyr Ile Glu Asn
85 90 95

Leu Gly Lys Pro Ile Glu Asn Tyr Thr Lys Ser Ile Thr Thr Ser Ser
100 105 110

Met Val Ser Gln Asp Ser Val Phe Pro Ala Pro Thr Ser Gly Gln Ile
115 120 125

Ser Trp Ser Leu Gln Cys Ala Glu Thr Ser His Phe Asn Gly Phe Leu
130 135 140

Ala Pro Glu Tyr Ala Ser Thr Pro Thr Ala Leu Pro His Leu Glu Met
145 150 155 160

Met Gly Leu Val Ser Ser Arg Val Pro Leu Pro His His Ile Gln Glu
165 170 175

Asn Glu Pro Ile Phe Val Asn Ala Lys Gln Tyr His Ala Ile Leu Arg
180 185 190

Arg Arg Lys His Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Ile Lys
195 200 205

Cys Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu His Ala Leu Lys
210 215 220

Arg Ala Arg Gly Ser Gly Gly Arg Phe Leu Asn Thr Lys Lys Leu Gln
225 230 235 240

Glu Ser Ser Asn Ser Leu Cys Ser Ser Gln Met Ala Asn Gly Gln Asn
245 250 255

Phe Ser Met Ser Pro His Gly Gly Gly Ser Gly Ile Gly Ser Ser Ser
260 265 270

Ile Ser Pro Ser Ser Asn Ser Asn Cys Ile Asn Met Phe Gln Asn Pro
275 280 285

Gln Phe Arg Phe Ser Gly Tyr Pro Ser Thr His His Ala Ser Ala Leu
290 295 300

Met Ser Gly Thr
305



203
25
DNA
synthetic construct

203

acagtacagt acagtacagt acagt 25



204
25
DNA
synthetic construct

204

actgtactgt actgtacgtg actgt 25



205
765
DNA
Oryza sativa

205

atggaggccg gctacccggg cgcggcggcg aacggcgctg ccgccgacgg gaacggtggc 60

gcgcagcagg cggcggccgc gccggctata cgtgagcagg accggctgat gccgatcgcg 120

aacgtgatcc gcatcatgcg ccgcgtgctc ccggcgcacg ccaagatctc ggacgacgcc 180

aaggagacga tccaggagtg cgtgtcggag tacatcagct tcatcaccgg ggaggccaac 240

gagcggtgcc agcgcgagca gcgcaagacc atcaccgccg aggacgtgct ctgggccatg 300

agccgcctcg gcttcgacga ctacgtcgag cccctcggcg tctacctcca ccgctaccgc 360

gagttcgagg gggagtcccg cggcgtcggc gtcggcgtcg gcgccgcgcg cggcgaccac 420

caccatggtc acgtcggtgg gatgctcaag tcccgcgcgc agggctccat ggtgacgcac 480

cacgacatgc agatgcacgc ggccatgtac ggtggcggcg cggtgccgcc gccgccgcat 540

cctcctccgc accaccacgc gttccaccag ctcatgccgc cgcaccacgg ccagtacgcg 600

ccgccgtacg acatgtacgg cggcgagcac gggatggcgg cgtactacgg cgggatgtac 660

gcgcccggca gcggcggcga cgggagcggc agcagcggca gcggtggcgc cggcacgccg 720

cagaccgtca acttcgagca ccagcatccg ttcggataca agtag 765



206
254
PRT
Oryza sativa

206

Met Glu Ala Gly Tyr Pro Gly Ala Ala Ala Asn Gly Ala Ala Ala Asp
1 5 10 15

Gly Asn Gly Gly Ala Gln Gln Ala Ala Ala Ala Pro Ala Ile Arg Glu
20 25 30

Gln Asp Arg Leu Met Pro Ile Ala Asn Val Ile Arg Ile Met Arg Arg
35 40 45

Val Leu Pro Ala His Ala Lys Ile Ser Asp Asp Ala Lys Glu Thr Ile
50 55 60

Gln Glu Cys Val Ser Glu Tyr Ile Ser Phe Ile Thr Gly Glu Ala Asn
65 70 75 80

Glu Arg Cys Gln Arg Glu Gln Arg Lys Thr Ile Thr Ala Glu Asp Val
85 90 95

Leu Trp Ala Met Ser Arg Leu Gly Phe Asp Asp Tyr Val Glu Pro Leu
100 105 110

Gly Val Tyr Leu His Arg Tyr Arg Glu Phe Glu Gly Glu Ser Arg Gly
115 120 125

Val Gly Val Gly Val Gly Ala Ala Arg Gly Asp His His His Gly His
130 135 140

Val Gly Gly Met Leu Lys Ser Arg Ala Gln Gly Ser Met Val Thr His
145 150 155 160

His Asp Met Gln Met His Ala Ala Met Tyr Gly Gly Gly Ala Val Pro
165 170 175

Pro Pro Pro His Pro Pro Pro His His His Ala Phe His Gln Leu Met
180 185 190

Pro Pro His His Gly Gln Tyr Ala Pro Pro Tyr Asp Met Tyr Gly Gly
195 200 205

Glu His Gly Met Ala Ala Tyr Tyr Gly Gly Met Tyr Ala Pro Gly Ser
210 215 220

Gly Gly Asp Gly Ser Gly Ser Ser Gly Ser Gly Gly Ala Gly Thr Pro
225 230 235 240

Gln Thr Val Asn Phe Glu His Gln His Pro Phe Gly Tyr Lys
245 250



207
1499
DNA
Catalpa speciosa

207

gcacgaggtg ctctttaaaa ttcacaagta catctgacct ctacatcaac acacattgac 60

tctaaattct ctctctaaat tctgtcaacc cccaaattct agggttttgt tttaattgtc 120

atcagatttc gccttaacag gacacattgg ttgatttctt tggagaaatt aggggagcat 180

gcaatccaag tcccagagcg gcaaccaagg agaatccaac ctttataatg ttcctaactc 240

caaagtaaat ccggattctt ggtggaataa tactggatat aattcctttt cctcaacaat 300

gatgggtgga aatgcatcag attcatcatc cctagaacaa tctgtggatg gacagtcgca 360

gtctaaaggt ggtataaatg aggaagatga tgatactacc aaacgatcac caagtagtac 420

acctctgctg ccagatagaa actataggca ggagggtccg agtctccagc aagctccacc 480

taccatacat ccaagaaaca atgggatcgt taatcaggcc ccacagcttg agcttggtgg 540

gcattcagta gcttgtgggt caaatcctta tgatccatat tacggaggaa tgatggcagc 600

ttatggccag ccattggttc ctcctcattt atatgatatg catcatgcaa ggatggcact 660

gcccctggag atgactcaag agcctgtata tgtgaatgcc aagcagtacc atggcattct 720

gcggaggcgg cagtctcgtg ctaaagctga gcttgaaaag aagttaataa aagttcggaa 780

gccttatctc catgagtctc gacaccaaca tgccttaagg agggcaaggg ggactggagg 840

acgatttgca aagaagtccg atgcagatac ttccaagggg actggacccg gctcatccat 900

cccatcgcag cttattagct catcacgagg ttctgagcca gtgcctgagg ctcagaattt 960

gtacaacgct gatgatggca attttagaag gcaaaccaac ttgcaggaac cggcacttca 1020

gttgggcaag acaggtgaag ggcccacttc aagtcacaag tggggaaata caacctcgaa 1080

ccatgcactt gctatgcagt aaagtcatac ttattggaag gtacaaatgc tggttacttg 1140

tttaaatctt ggctttccca agctgagcgg caattcattc ttggctgttt ctattttatc 1200

tcgtggagga ggaaggatga gagtctttgt ttcttagctt ctcttaatgt ctattgttct 1260

tcccttgtgt acaaaatgtc ttttagcatt agaggcaaag tttgagttag gacaagacaa 1320

ccgaagtttg ggtagggaaa acttggttta taacttaaga ttcttgtaaa gttccgcaag 1380

gagtcgcatg catgtgtttg ctacttacat ttgttgcact ttcgaattgt gaacccaaaa 1440

gcatcaatgg tgtttgaata gaacttttaa aagccaaaaa aaaaaaaaaa aaaaaaaaa 1499



208
307
PRT
Catalpa speciosa

208

Met Gln Ser Lys Ser Gln Ser Gly Asn Gln Gly Glu Ser Asn Leu Tyr
1 5 10 15

Asn Val Pro Asn Ser Lys Val Asn Pro Asp Ser Trp Trp Asn Asn Thr
20 25 30

Gly Tyr Asn Ser Phe Ser Ser Thr Met Met Gly Gly Asn Ala Ser Asp
35 40 45

Ser Ser Ser Leu Glu Gln Ser Val Asp Gly Gln Ser Gln Ser Lys Gly
50 55 60

Gly Ile Asn Glu Glu Asp Asp Asp Thr Thr Lys Arg Ser Pro Ser Ser
65 70 75 80

Thr Pro Leu Leu Pro Asp Arg Asn Tyr Arg Gln Glu Gly Pro Ser Leu
85 90 95

Gln Gln Ala Pro Pro Thr Ile His Pro Arg Asn Asn Gly Ile Val Asn
100 105 110

Gln Ala Pro Gln Leu Glu Leu Gly Gly His Ser Val Ala Cys Gly Ser
115 120 125

Asn Pro Tyr Asp Pro Tyr Tyr Gly Gly Met Met Ala Ala Tyr Gly Gln
130 135 140

Pro Leu Val Pro Pro His Leu Tyr Asp Met His His Ala Arg Met Ala
145 150 155 160

Leu Pro Leu Glu Met Thr Gln Glu Pro Val Tyr Val Asn Ala Lys Gln
165 170 175

Tyr His Gly Ile Leu Arg Arg Arg Gln Ser Arg Ala Lys Ala Glu Leu
180 185 190

Glu Lys Lys Leu Ile Lys Val Arg Lys Pro Tyr Leu His Glu Ser Arg
195 200 205

His Gln His Ala Leu Arg Arg Ala Arg Gly Thr Gly Gly Arg Phe Ala
210 215 220

Lys Lys Ser Asp Ala Asp Thr Ser Lys Gly Thr Gly Pro Gly Ser Ser
225 230 235 240

Ile Pro Ser Gln Leu Ile Ser Ser Ser Arg Gly Ser Glu Pro Val Pro
245 250 255

Glu Ala Gln Asn Leu Tyr Asn Ala Asp Asp Gly Asn Phe Arg Arg Gln
260 265 270

Thr Asn Leu Gln Glu Pro Ala Leu Gln Leu Gly Lys Thr Gly Glu Gly
275 280 285

Pro Thr Ser Ser His Lys Trp Gly Asn Thr Thr Ser Asn His Ala Leu
290 295 300

Ala Met Gln
305



209
1626
DNA
Zea mays

209

ccacgcgtcc gcgatcagcg tcagttacca cgacgaccga tcttgctcgc cagcgagagc 60

gacccctccc ctccctactt cccatgctga tctcggcgcg cttctcttcc tcctccccca 120

gagccgggca ctgatttccc ttggctgctg ctgctggatt ctttggtgtt ccatcaggcc 180

aaggatcccg caaagagctc cggagccaag cctgctgcag ccgtcgcgtc gggtgaggca 240

ggcttcagct tcagtctcct actcgacgag gcaagcggat cggagcgggc ctccgctccg 300

ccatgatgag cttcaagggc cacgaggggt ttggccaggt ggccgccgcc ggtgccggga 360

gccaggctgc ctcccatggt ggagcaggcc cgctgccatg gtgggcgggg ccccagctgc 420

tgttcggcga gccggcgccc ccgtcaccgg aggagacgcg ccgggacgcc cagttccagg 480

tcgtgccggg ggttcagggc acgccggatc cagcgccgcc caagacaggg acacctgagg 540

tcctcaaatt ctctgtcttt caagggaatt tggagtcggg tggtaaagga gagaaaaccc 600

caaagaactc taccactatt gctcttcagt caccgttccc agaatacaat ggccgtttcg 660

agattggtct tggtcaatct atgctggccc cttccaatta tccttgtgct gaccagtgct 720

atggcatgct tgcggcttat ggaatgagat cgatgtctgg tgggagaatg ctgttgccac 780

taaatgcgac agctgatgca cccatctatg tgaatccgaa gcagtacgaa ggcatcctcc 840

gccgtcgccg tgctcgcgcc aaggcggaga gcgagaacag gctcgccaaa ggcagaaagc 900

cctatctcca cgagtcgcgc cacctccacg cgatgcgtcg ggtaagaggc accggcgggc 960

gcttcgtcaa cacgaagaaa gaagggcgtg gcacgggcgt tgcttcgaac gggggcagca 1020

agacggctgc agcggcaccg tcgcgcctcg ccatgccccc tagcttccag agtagcgtcg 1080

ccagcctgtc tggctccgac gtgtcaaaca tgtacagcgg cggcttggag cagcaccttc 1140

gggcgccgca cttcttcacc ccgctgccac ccatcatgga ggacggcgac cacggtggtc 1200

cccccacccg catctcctcc tccttcaagt gggcagccag cgacggctgc tgcgagctcc 1260

tcaaggcgtg aaccgacgag gaggagggga tggctactca gacgaacggc cttctcgccg 1320

atggctggtc gtctgtaggc aaatcattct tggctgttcc gcattggggt gcaacctcat 1380

ccacatcatc tacctaccca gtaggccagt accccctgtt ccctgaacag tgcttgggtt 1440

acaggggtcc tcctgtgtgt gtgatgatgt ggtgtgcctc ccccacatgc atttgctgta 1500

acataatagt gtacccaaac cactgcttcg gactatcatt gtctgtctcg gtatggattc 1560

tctgttgtca cagtgtctga ataattgagg cgtcagactt caaagttaaa aaaaaaaaaa 1620

aaaaaa 1626



210
322
PRT
Zea mays

210

Met Met Ser Phe Lys Gly His Glu Gly Phe Gly Gln Val Ala Ala Ala
1 5 10 15

Gly Ala Gly Ser Gln Ala Ala Ser His Gly Gly Ala Gly Pro Leu Pro
20 25 30

Trp Trp Ala Gly Pro Gln Leu Leu Phe Gly Glu Pro Ala Pro Pro Ser
35 40 45

Pro Glu Glu Thr Arg Arg Asp Ala Gln Phe Gln Val Val Pro Gly Val
50 55 60

Gln Gly Thr Pro Asp Pro Ala Pro Pro Lys Thr Gly Thr Pro Glu Val
65 70 75 80

Leu Lys Phe Ser Val Phe Gln Gly Asn Leu Glu Ser Gly Gly Lys Gly
85 90 95

Glu Lys Thr Pro Lys Asn Ser Thr Thr Ile Ala Leu Gln Ser Pro Phe
100 105 110

Pro Glu Tyr Asn Gly Arg Phe Glu Ile Gly Leu Gly Gln Ser Met Leu
115 120 125

Ala Pro Ser Asn Tyr Pro Cys Ala Asp Gln Cys Tyr Gly Met Leu Ala
130 135 140

Ala Tyr Gly Met Arg Ser Met Ser Gly Gly Arg Met Leu Leu Pro Leu
145 150 155 160

Asn Ala Thr Ala Asp Ala Pro Ile Tyr Val Asn Pro Lys Gln Tyr Glu
165 170 175

Gly Ile Leu Arg Arg Arg Arg Ala Arg Ala Lys Ala Glu Ser Glu Asn
180 185 190

Arg Leu Ala Lys Gly Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu
195 200 205

His Ala Met Arg Arg Val Arg Gly Thr Gly Gly Arg Phe Val Asn Thr
210 215 220

Lys Lys Glu Gly Arg Gly Thr Gly Val Ala Ser Asn Gly Gly Ser Lys
225 230 235 240

Thr Ala Ala Ala Ala Pro Ser Arg Leu Ala Met Pro Pro Ser Phe Gln
245 250 255

Ser Ser Val Ala Ser Leu Ser Gly Ser Asp Val Ser Asn Met Tyr Ser
260 265 270

Gly Gly Leu Glu Gln His Leu Arg Ala Pro His Phe Phe Thr Pro Leu
275 280 285

Pro Pro Ile Met Glu Asp Gly Asp His Gly Gly Pro Pro Thr Arg Ile
290 295 300

Ser Ser Ser Phe Lys Trp Ala Ala Ser Asp Gly Cys Cys Glu Leu Leu
305 310 315 320

Lys Ala



211
1569
DNA
Oryza sativa

211

gcacgaggcg atctttcccc agagagagag agagagagag agagagtctt gattggggga 60

ggagagaggg agagagagaa agagagagga cagaaaatgt ttgtggatct tgagtaatgc 120

cttctaataa tgataatgct gttgcaagaa atggagaatc atcctgtcca atgcatggcc 180

aagaccaact atgattttct tgccaggaat aactatccaa tgaaacagtt agttcagagg 240

aactctgatg gtgactcgtc accaacaaag tctggggagt ctcaccaaga agcatctgca 300

gtaagtgaca gcagtctcaa cggacaacac acctcaccac aatcagtgtt tgtcccctca 360

gatattaaca acaatgatag ttgtggggag cgggaccatg gcactaagtc ggtattgtct 420

ttgggcaaca cagaagctgc ctttcctcct tcaaagttcg attacaacca gccttttgca 480

tgtgtttctt atccatatgg tactgatcca tattatggtg gagtattaac aggatacact 540

tcacatgcat ttgttcatcc tcaaattact ggtgctgcaa actctaggat gccattgcct 600

gttgatcctt ctgtagaaga gcccatattt gtcaatgcaa agcaatacaa tgcgatcctt 660

agaagaaggc aaacgcgtgc aaaattggag gcccaaaata aggcggtgaa aggtcggaag 720

ccttacctcc atgaatctcg acatcatcat gctatgaagc gagcccgtgg atcaggtggt 780

cggyyactta ccaaaaagga gctgctggaa cagcagcagc agcagcagca gcagaagcca 840

ccaccggcat cagctcagtc tccaacaggt agagccagaa cgagcggcgg tgccgttgtc 900

cttggcaaga acctgtgccc agagaacagc acatcctgct cgccatcgac accgacaggc 960

tccgagatct ccagcatctc atttgggggc ggcatgctgg ctcaccaaga gcacatcagc 1020

ttcgcatccg ctgatcgcca ccccacaatg aaccagaacc accgtgtccc cgtcatgagg 1080

tgaaaacctc gggatcgcgg gacacgggcg gttctggttt accctcactg gcgcactccg 1140

gtgtgcccgt ggcaattcat ccttggctta tgaagtatct acctgataat agtctgctgt 1200

cagtttatat gcaatgcaac ctctgtcaga taaactctta tagtttgttt tattgtaagc 1260

tatgactgaa cgaactgtcg agcagatggc taatttgtat gttgtgggta cagaaatcct 1320

gaagcttttg atgtacctaa ttgccttttg cttatactct tggtgtatac ccattaccaa 1380

gttgccttaa aaaccctcca attatgtaat cagtcatggt tttatagaac cttgccacat 1440

gtaatcaatc acctgttttt gtaaattgat ctataaacgc taaaaaaaaa aaaaaaaaaa 1500

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1560

aaaaaaaaa 1569



212
317
PRT
Oryza sativa

212

Met Ile Met Leu Leu Gln Glu Met Glu Asn His Pro Val Gln Cys Met
1 5 10 15

Ala Lys Thr Asn Tyr Asp Phe Leu Ala Arg Asn Asn Tyr Pro Met Lys
20 25 30

Gln Leu Val Gln Arg Asn Ser Asp Gly Asp Ser Ser Pro Thr Lys Ser
35 40 45

Gly Glu Ser His Gln Glu Ala Ser Ala Val Ser Asp Ser Ser Leu Asn
50 55 60

Gly Gln His Thr Ser Pro Gln Ser Val Phe Val Pro Ser Asp Ile Asn
65 70 75 80

Asn Asn Asp Ser Cys Gly Glu Arg Asp His Gly Thr Lys Ser Val Leu
85 90 95

Ser Leu Gly Asn Thr Glu Ala Ala Phe Pro Pro Ser Lys Phe Asp Tyr
100 105 110

Asn Gln Pro Phe Ala Cys Val Ser Tyr Pro Tyr Gly Thr Asp Pro Tyr
115 120 125

Tyr Gly Gly Val Leu Thr Gly Tyr Thr Ser His Ala Phe Val His Pro
130 135 140

Gln Ile Thr Gly Ala Ala Asn Ser Arg Met Pro Leu Pro Val Asp Pro
145 150 155 160

Ser Val Glu Glu Pro Ile Phe Val Asn Ala Lys Gln Tyr Asn Ala Ile
165 170 175

Leu Arg Arg Arg Gln Thr Arg Ala Lys Leu Glu Ala Gln Asn Lys Ala
180 185 190

Val Lys Gly Arg Lys Pro Tyr Leu His Glu Ser Arg His His His Ala
195 200 205

Met Lys Arg Ala Arg Gly Ser Gly Gly Arg Phe Leu Thr Lys Lys Glu
210 215 220

Leu Leu Glu Gln Gln Gln Gln Gln Gln Gln Gln Lys Pro Pro Pro Ala
225 230 235 240

Ser Ala Gln Ser Pro Thr Gly Arg Ala Arg Thr Ser Gly Gly Ala Val
245 250 255

Val Leu Gly Lys Asn Leu Cys Pro Glu Asn Ser Thr Ser Cys Ser Pro
260 265 270

Ser Thr Pro Thr Gly Ser Glu Ile Ser Ser Ile Ser Phe Gly Gly Gly
275 280 285

Met Leu Ala His Gln Glu His Ile Ser Phe Ala Ser Ala Asp Arg His
290 295 300

Pro Thr Met Asn Gln Asn His Arg Val Pro Val Met Arg
305 310 315



213
1375
DNA
Glycine max

213

gcacgaggag gttgcagact tagaaagaga gagagagaga gaatgggtct catctcaatg 60

caatttaggt tctgaaaacc aaagcttttc ataggaaaag ttgtgctaag atgccaggga 120

aacctgacac tgatgattgg cgtgtagagc gtggggagca gattcagttt cagtcttcca 180

tttactctca tcatcagcct tggtggcgcg gagtggggga aaatgcctcc aaatcatctt 240

cagatgatca gttaaatggt tcaatcgtga atggtatcac gcggtctgag accaatgata 300

agtcaggcgg aggtgttgcc aaagaatacc aaaacatcaa acatgccatg ttgtcaaccc 360

catttaccat ggagaaacat cttgctccaa atccccagat ggaacttgtt ggtcattcag 420

ttgttttaac atctccttat tcagatgcac agtatggtca aatcttgact acttacgggc 480

aacaagttat gataaatcct cagttgtatg gaatgcatca tgctagaatg cctttgccac 540

ttgaaatgga agaggagcct gtttatgtca atgcgaagca gtatcatggt attttgaggc 600

gaagacagtc acgtgctaag gctgagattg aaaagaaagt aatcaaaaac aggaagccat 660

acctccatga atcccgtcac cttcatgcaa tgagaagggc aagaggcaac ggtggtcgct 720

ttctcaacac aaagaagctt gaaaataaca attctaattc cacttcagac aaaggcaaca 780

atactcgtgc aaacgcctca acaaactcgc ctaacactca acttttgttc accaacaatt 840

tgaatctagg ctcatcaaat gtttcacaag ccacagttca gcacatgcac acagagcaga 900

gtttcactat aggttaccat aatggaaatg gtcttacagc actataccgt tcacaagcaa 960

atgggaaaaa ggagggaaac tgctttggta aagagaggga ccctaatggg gatttcaaat 1020

aacacttccc tcagccatac agcaagagtg aagatgaagg gctttatctc atccaacttg 1080

tgatgctgta tagaaggcaa ttcattcttg gcttagttaa gtggtgagac cagtgacatg 1140

gtgtacacta tggccttgtt tggtctctcc cttgcttttg tttctctcta caagtccata 1200

tgtaaaatgg ataacagaaa gaaaaagaaa aatcactttg gtttgagaac tttttaaagt 1260

ttatattaac tgtgttaagg ttcataaaac tgtagactga tttgtgtgac atgctccaca 1320

gaaccttaaa ttttcctcta ttttgtccta aaaaaaaaaa aaaaaaaaaa aaaaa 1375



214
303
PRT
Glycine max

214

Met Pro Gly Lys Pro Asp Thr Asp Asp Trp Arg Val Glu Arg Gly Glu
1 5 10 15

Gln Ile Gln Phe Gln Ser Ser Ile Tyr Ser His His Gln Pro Trp Trp
20 25 30

Arg Gly Val Gly Glu Asn Ala Ser Lys Ser Ser Ser Asp Asp Gln Leu
35 40 45

Asn Gly Ser Ile Val Asn Gly Ile Thr Arg Ser Glu Thr Asn Asp Lys
50 55 60

Ser Gly Gly Gly Val Ala Lys Glu Tyr Gln Asn Ile Lys His Ala Met
65 70 75 80

Leu Ser Thr Pro Phe Thr Met Glu Lys His Leu Ala Pro Asn Pro Gln
85 90 95

Met Glu Leu Val Gly His Ser Val Val Leu Thr Ser Pro Tyr Ser Asp
100 105 110

Ala Gln Tyr Gly Gln Ile Leu Thr Thr Tyr Gly Gln Gln Val Met Ile
115 120 125

Asn Pro Gln Leu Tyr Gly Met His His Ala Arg Met Pro Leu Pro Leu
130 135 140

Glu Met Glu Glu Glu Pro Val Tyr Val Asn Ala Lys Gln Tyr His Gly
145 150 155 160

Ile Leu Arg Arg Arg Gln Ser Arg Ala Lys Ala Glu Ile Glu Lys Lys
165 170 175

Val Ile Lys Asn Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu His
180 185 190

Ala Met Arg Arg Ala Arg Gly Asn Gly Gly Arg Phe Leu Asn Thr Lys
195 200 205

Lys Leu Glu Asn Asn Asn Ser Asn Ser Thr Ser Asp Lys Gly Asn Asn
210 215 220

Thr Arg Ala Asn Ala Ser Thr Asn Ser Pro Asn Thr Gln Leu Leu Phe
225 230 235 240

Thr Asn Asn Leu Asn Leu Gly Ser Ser Asn Val Ser Gln Ala Thr Val
245 250 255

Gln His Met His Thr Glu Gln Ser Phe Thr Ile Gly Tyr His Asn Gly
260 265 270

Asn Gly Leu Thr Ala Leu Tyr Arg Ser Gln Ala Asn Gly Lys Lys Glu
275 280 285

Gly Asn Cys Phe Gly Lys Glu Arg Asp Pro Asn Gly Asp Phe Lys
290 295 300



215
1192
DNA
Triticum aestivum

215

gcacgaggga gtgacgcggt cgaggagggg cgtgcggggg gcagacagag agggagcgca 60

aagggacggc ggaggcaagc tagcttcccg ggggcggacg caccgagaga gggcggcggg 120

agggaggagg cgcgtgggag ccatgcttct cccctcttct tcgtcttcct cctacgatcc 180

caaaggtgac tcctttggga aatcggttga cgatcatatg aggtcaactt tgacttttgg 240

tgataagcat tctgtatttg caagtcaaaa cactgactat ggccacccaa tggcttgcat 300

ttcataccca ttcaatgatt ctggttctgt ttgggcggcc tatgggtcac gggctatgtt 360

ccagcccctc atggcggaag gaggggcatc tgcgaccgca agagttccat tgcctgtcga 420

attagcagcg gatgagccca tatttgtcaa tcccaaacaa tataatggga ttctccggcg 480

aaggcagctg cgcgccaagt tagaggccca gaataaactc acaaaaaaca gaaagcccta 540

cctccacgag tctcgccatc ttcacgcgat gaagcgggca agaggttccg ggggacgttt 600

cctcaattcc aaacagctga agcagcagca gcagcagtct ggcagtgcat gcacgaaggc 660

cattgcggat ggcgcgaatt ccttgggttc aacccatcta cggctaggca gcggcgcagc 720

cggagaccga agcaactcgg cgtccaaggc gatgtcctcc caagagaaca gcaagagagt 780

cgccgccccg gctcccgcct tcaccatgat tcaagcggcg cgcaaagacg acgacttctt 840

ccaccatcac ggccaccatc tcagcttctc cgaccacttc ggccagtcga gcgaccggta 900

tacgtaacaa ggggtcctct gtgccccggt gtggtctggc aactcatcct tggctttatt 960

tctggcgtgt tagggtttca gagatagtgt atctcatagt actactgttg tactgctttg 1020

cacccacata gttctctgct tgatgttcgg catgcaaatg ttggtgtact ggtgcgttgg 1080

gacaaaagtt tgatgtgttt acatgacaat tggtcgcgga actcatcttg tgttctgctc 1140

gactctaatg tgtgtgctca catgtgaatt ccgtaaaaaa aaaaaaaaaa aa 1192



216
254
PRT
Triticum aestivum

216

Met Leu Leu Pro Ser Ser Ser Ser Ser Ser Tyr Asp Pro Lys Gly Asp
1 5 10 15

Ser Phe Gly Lys Ser Val Asp Asp His Met Arg Ser Thr Leu Thr Phe
20 25 30

Gly Asp Lys His Ser Val Phe Ala Ser Gln Asn Thr Asp Tyr Gly His
35 40 45

Pro Met Ala Cys Ile Ser Tyr Pro Phe Asn Asp Ser Gly Ser Val Trp
50 55 60

Ala Ala Tyr Gly Ser Arg Ala Met Phe Gln Pro Leu Met Ala Glu Gly
65 70 75 80

Gly Ala Ser Ala Thr Ala Arg Val Pro Leu Pro Val Glu Leu Ala Ala
85 90 95

Asp Glu Pro Ile Phe Val Asn Pro Lys Gln Tyr Asn Gly Ile Leu Arg
100 105 110

Arg Arg Gln Leu Arg Ala Lys Leu Glu Ala Gln Asn Lys Leu Thr Lys
115 120 125

Asn Arg Lys Pro Tyr Leu His Glu Ser Arg His Leu His Ala Met Lys
130 135 140

Arg Ala Arg Gly Ser Gly Gly Arg Phe Leu Asn Ser Lys Gln Leu Lys
145 150 155 160

Gln Gln Gln Gln Gln Ser Gly Ser Ala Cys Thr Lys Ala Ile Ala Asp
165 170 175

Gly Ala Asn Ser Leu Gly Ser Thr His Leu Arg Leu Gly Ser Gly Ala
180 185 190

Ala Gly Asp Arg Ser Asn Ser Ala Ser Lys Ala Met Ser Ser Gln Glu
195 200 205

Asn Ser Lys Arg Val Ala Ala Pro Ala Pro Ala Phe Thr Met Ile Gln
210 215 220

Ala Ala Arg Lys Asp Asp Asp Phe Phe His His His Gly His His Leu
225 230 235 240

Ser Phe Ser Asp His Phe Gly Gln Ser Ser Asp Arg Tyr Thr
245 250



217
298
PRT
Arabidopsis thaliana

misc_feature

gi 9293997


217

Met His Ser Lys Ser Asp Ser Gly Gly Asn Lys Val Asp Ser Glu Val
1 5 10 15

His Gly Thr Val Ser Ser Ser Ile Asn Ser Leu Asn Pro Trp His Arg
20 25 30

Ala Ala Ala Ala Cys Asn Ala Asn Ser Ser Val Glu Ala Gly Asp Lys
35 40 45

Ser Ser Lys Ser Ile Ala Leu Ala Leu Glu Ser Asn Gly Ser Lys Ser
50 55 60

Pro Ser Asn Arg Asp Asn Thr Val Asn Lys Glu Ser Gln Val Thr Thr
65 70 75 80

Ser Pro Gln Ser Ala Gly Asp Tyr Ser Asp Lys Asn Gln Glu Ser Leu
85 90 95

His His Gly Ile Thr Gln Pro Pro Pro His Pro Gln Leu Val Gly His
100 105 110

Thr Val Gly Trp Ala Ser Ser Asn Pro Tyr Gln Asp Pro Tyr Tyr Ala
115 120 125

Gly Val Met Gly Ala Tyr Gly His His Pro Leu Gly Phe Val Pro Tyr
130 135 140

Gly Gly Met Pro His Ser Arg Met Pro Leu Pro Pro Glu Met Ala Gln
145 150 155 160

Glu Pro Val Phe Val Asn Ala Lys Gln Tyr Gln Ala Ile Leu Arg Arg
165 170 175

Arg Gln Ala Arg Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys Ser
180 185 190

Arg Lys Pro Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg
195 200 205

Pro Arg Gly Thr Gly Gly Arg Phe Ala Lys Lys Thr Asn Thr Glu Ala
210 215 220

Ser Lys Arg Lys Ala Glu Glu Lys Ser Asn Gly His Val Thr Gln Ser
225 230 235 240

Pro Ser Ser Ser Asn Ser Asp Gln Gly Glu Ala Trp Asn Gly Asp Tyr
245 250 255

Arg Thr Pro Gln Gly Asp Glu Met Gln Ser Ser Ala Tyr Lys Arg Arg
260 265 270

Glu Glu Gly Glu Cys Ser Gly Gln Gln Trp Asn Ser Leu Ser Ser Asn
275 280 285

His Pro Ser Gln Ala Arg Leu Ala Ile Lys
290 295



218
238
PRT
Oryza sativa

misc_feature

gi 7489565


218

Met Leu Pro Pro His Leu Thr Glu Asn Gly Thr Val Met Ile Gln Phe
1 5 10 15

Gly His Lys Met Pro Asp Tyr Glu Ser Ser Ala Thr Gln Ser Thr Ser
20 25 30

Gly Ser Pro Arg Glu Val Ser Gly Met Ser Glu Gly Ser Leu Asn Glu
35 40 45

Gln Asn Asp Gln Ser Gly Asn Leu Asp Gly Tyr Thr Lys Ser Asp Glu
50 55 60

Gly Lys Met Met Ser Ala Leu Ser Leu Gly Lys Ser Glu Thr Val Tyr
65 70 75 80

Ala His Ser Glu Pro Asp Arg Ser Gln Pro Phe Gly Ile Ser Tyr Pro
85 90 95

Tyr Ala Asp Ser Phe Tyr Gly Gly Ala Val Ala Thr Tyr Gly Thr His
100 105 110

Ala Ile Met His Pro Gln Ile Val Gly Val Met Ser Ser Ser Arg Val
115 120 125

Pro Leu Pro Ile Glu Pro Ala Thr Glu Glu Pro Ile Tyr Val Asn Ala
130 135 140

Lys Gln Tyr His Ala Ile Leu Arg Arg Arg Gln Leu Arg Ala Lys Leu
145 150 155 160

Glu Ala Glu Asn Lys Leu Val Lys Asn Arg Lys Pro Tyr Leu His Glu
165 170 175

Ser Arg His Gln His Ala Met Lys Arg Ala Arg Gly Thr Gly Gly Arg
180 185 190

Phe Leu Asn Thr Lys Gln Gln Pro Glu Ala Ser Asp Gly Gly Thr Pro
195 200 205

Arg Leu Val Ser Ala Asn Gly Val Val Phe Ser Lys His Glu His Ser
210 215 220

Leu Ser Ser Ser Asp Leu His His Arg Ala Lys Glu Gly Ala
225 230 235



219
215
PRT
Vitis riparia

misc_feature

gi 7141243


219

Met Met Pro Met Thr Met Ala Glu Tyr His Leu Ala Pro Pro Ser Gln
1 5 10 15

Leu Glu Leu Val Gly His Ser Ile Val Gln Ser Gln Phe Leu Gly Val
20 25 30

Asn Val Ala Arg Met Ala Leu Pro Ile Glu Met Ala Glu Glu Pro Val
35 40 45

Tyr Val Asn Ala Lys Gln Tyr His Gly Ile Leu Arg Arg Arg Gln Ser
50 55 60

Arg Ala Lys Ala Glu Leu Glu Lys Lys Leu Ile Lys Val Arg Lys Pro
65 70 75 80

Tyr Leu His Glu Ser Arg His Gln His Ala Met Arg Arg Ala Arg Gly
85 90 95

Cys Gly Gly Arg Phe Leu Asn Thr Lys Lys Leu Asp Ser Asn Ala Ser
100 105 110

Tyr Asp Met Pro Asp Lys Gly Ser Asp Pro Asp Val Asn Leu Ser Thr
115 120 125

Arg Pro Ile Ser Ser Ser Val Ser Glu Ser Leu Pro Phe Asn Ser Ser
130 135 140

Arg Asn Glu Asp Ser Pro Thr Ser His Leu Asp Ala Arg Gly Pro Ser
145 150 155 160

Val Gln Glu Leu His Asn Arg Gln Thr Ser Ser Met Glu Met Ala Thr
165 170 175

Ser Leu Leu Ser Thr Gln Pro Gly Ile Ser Val Gly Arg Thr Tyr His
180 185 190

Ser Leu Lys Met Met Ile Gly Val Glu Arg Arg Arg Pro Arg Lys Ala
195 200 205

Ala Ser Ile Arg Glu Phe Trp
210 215



220
1329
DNA
Glycine max

220

gcacgagtag ggttttctcc tcccccattg acccaccgtc catcgcaaag gaagtcgcgc 60

ccaatttcca tggtttgtag attaaatctt aaagcagtaa gtcatcatgg ataaatcaga 120

gcagactcag cagcaacatc agcatgggat gggcgttgcc acaggtgcta gccaaatggc 180

ctattcttct cactacccga ctgctcccat ggtggcttct ggcacgcctg ctgtagctgt 240

tccttcccca actcaggctc cagctgcctt ctctagttct gctcaccagc ttgcatacca 300

gcaagcacag catttccacc accaacagca gcaacaccaa caacagcagc ttcaaatgtt 360

ctggtcaaac caaatgcaag aaattgagca aacaattgac tttaaaaacc acagtcttcc 420

tcttgctcgg ataaaaaaga taatgaaagc tgatgaagat gtccggatga tttctgcaga 480

agctccagtc atatttgcaa aagcatgtga aatgttcata ttagagttga cgttgagatc 540

ttggatccac acagaagaga acaagaggag aactctacaa aagaatgata tagcagctgc 600

tatttcgaga aacgatgttt ttgatttctt ggttgatatt atcccaagag atgagttgaa 660

agaggaagga cttggaataa ccaaggctac tattccattg gtgaattctc cagctgatat 720

gccatattac tatgtccctc cacagcatcc tgttgtagga cctcctggga tgatcatggg 780

caagcccgtt ggtgctgagc aagcaacgct gtattctaca cagcagcctc gacctcccat 840

ggcgttcatg ccatggcccc atacacaacc ccagcaacag cagccacccc aacatcaaca 900

aacagactca tgatgacaat gcaattcaat taggtcggaa agtagcatgc accttatgat 960

tattacaaat ttacttaatg cctttaagtc agctgtagtt tagtgttttg cattgaaaaa 1020

tgccaaagat tgtttgaggt ttcttgcact catttatgat tgtatgagct cttatgctga 1080

gttacttttg gttgtgttta tttgaggtac tggtgtggta gttaaattag tttgtagctg 1140

tccataagta aacagcgtag ctgcttaatt aggaggtctg aaatgatgaa atagtttgta 1200

ttgttattgc agaaggtagg ttttattcag tatttcaaaa aaaaaaaaaa aaaaaaaaaa 1260

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1320

aaaaaaaaaa 1329



221
268
PRT
Glycine max

221

Met Asp Lys Ser Glu Gln Thr Gln Gln Gln His Gln His Gly Met Gly
1 5 10 15

Val Ala Thr Gly Ala Ser Gln Met Ala Tyr Ser Ser His Tyr Pro Thr
20 25 30

Ala Pro Met Val Ala Ser Gly Thr Pro Ala Val Ala Val Pro Ser Pro
35 40 45

Thr Gln Ala Pro Ala Ala Phe Ser Ser Ser Ala His Gln Leu Ala Tyr
50 55 60

Gln Gln Ala Gln His Phe His His Gln Gln Gln Gln His Gln Gln Gln
65 70 75 80

Gln Leu Gln Met Phe Trp Ser Asn Gln Met Gln Glu Ile Glu Gln Thr
85 90 95

Ile Asp Phe Lys Asn His Ser Leu Pro Leu Ala Arg Ile Lys Lys Ile
100 105 110

Met Lys Ala Asp Glu Asp Val Arg Met Ile Ser Ala Glu Ala Pro Val
115 120 125

Ile Phe Ala Lys Ala Cys Glu Met Phe Ile Leu Glu Leu Thr Leu Arg
130 135 140

Ser Trp Ile His Thr Glu Glu Asn Lys Arg Arg Thr Leu Gln Lys Asn
145 150 155 160

Asp Ile Ala Ala Ala Ile Ser Arg Asn Asp Val Phe Asp Phe Leu Val
165 170 175

Asp Ile Ile Pro Arg Asp Glu Leu Lys Glu Glu Gly Leu Gly Ile Thr
180 185 190

Lys Ala Thr Ile Pro Leu Val Asn Ser Pro Ala Asp Met Pro Tyr Tyr
195 200 205

Tyr Val Pro Pro Gln His Pro Val Val Gly Pro Pro Gly Met Ile Met
210 215 220

Gly Lys Pro Val Gly Ala Glu Gln Ala Thr Leu Tyr Ser Thr Gln Gln
225 230 235 240

Pro Arg Pro Pro Met Ala Phe Met Pro Trp Pro His Thr Gln Pro Gln
245 250 255

Gln Gln Gln Pro Pro Gln His Gln Gln Thr Asp Ser
260 265



222
199
PRT
Arabidopsis thaliana

misc_feature

gi 15223482


222

Met Glu Gln Ser Glu Glu Gly Gln Gln Gln Gln Gln Gln Gly Val Met
1 5 10 15

Asp Tyr Val Pro Pro His Ala Tyr Gln Ser Gly Pro Val Asn Ala Ala
20 25 30

Ser His Met Ala Phe Gln Gln Ala His His Phe His His His His Gln
35 40 45

Gln Gln Gln Gln Gln Gln Leu Gln Met Phe Trp Ala Asn Gln Met Gln
50 55 60

Glu Ile Glu His Thr Thr Asp Phe Lys Asn His Thr Leu Pro Leu Ala
65 70 75 80

Arg Ile Lys Lys Ile Met Lys Ala Asp Glu Asp Val Arg Met Ile Ser
85 90 95

Ala Glu Ala Pro Val Ile Phe Ala Lys Ala Cys Glu Met Phe Ile Leu
100 105 110

Glu Leu Thr Leu Arg Ala Trp Ile His Thr Glu Glu Asn Lys Arg Arg
115 120 125

Thr Leu Gln Lys Asn Asp Ile Ala Ala Ala Ile Ser Arg Thr Asp Val
130 135 140

Phe Asp Phe Leu Val Asp Ile Ile Pro Arg Asp Glu Leu Lys Glu Glu
145 150 155 160

Gly Leu Gly Val Thr Lys Gly Thr Ile Pro Ser Val Val Gly Ser Pro
165 170 175

Pro Tyr Tyr Tyr Leu Gln Gln Gln Gly Met Met Gln His Trp Pro Gln
180 185 190

Glu Gln His Pro Asp Glu Ser
195