(en)L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.

1.ApplicationNumber: US-6245802-A
1.PublishNumber: US-2003003550-A1
2.Date Publish: 20030102
3.Inventor: NAKAMURA JUN
IZUI HIROSHI
MORIGUCHI KAYO
KAWASHIMA HIROKI
NAKAMATSU TSUYOSHI
KURAHASHI OSAMU

4.Inventor Harmonized: NAKAMURA JUN(JP)
IZUI HIROSHI(JP)
MORIGUCHI KAYO(JP)
KAWASHIMA HIROKI(JP)
NAKAMATSU TSUYOSHI(JP)
KURAHASHI OSAMU(JP)

5.Country: US
6.Claims:
(en)L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.

7.Description:
(en)BACKGROUND OF THE INVENTION
[0001] 1. Field of the Invention
[0002] The present invention relates to an L-glutamine producing bacterium belonging to coryneform bacteria and a method for producing L-glutamine. L-Glutamine is an industrially useful amino acid as an ingredient of seasonings, liver function promoting agents, amino acid transfusions, comprehensive amino acid preparations and so forth.
[0003] 2. Related Art
[0004] In order to produce L-amino acids by fermentation, methods for improving microorganisms by breeding have been used abundantly. That is, since production ability of wild strains per se for L-amino acid production is extremely low in many cases, there have been known methods of imparting auxotrophy or analogue resistance by mutation or imparting mutation for metabolic regulation and methods utilizing a combination of these. Although L-glutamine can be obtained with an appropriate yield by the aforementioned methods, it is indispensable to further improve the fermentation yield in order to industrially produce L-glutamine at a low cost.
[0005] Further, the L-glutamine fermentation also suffers from the problem of by-production of L-glutamic acid. A method for solving this problem is proposed in, for example, Japanese Patent Laid-open Publication (Kokai) No. 3-232497. Although the production of L-glutamic acid can be suppressed to a certain extent by this method, there is still by-production of L-glutamic acid and the yield of L-glutamine is insufficient.
[0006] Since such improvements of L-glutamine producing bacteria as mentioned above utilize methods of treating a host bacterium with a mutagenizing agent or the like and selecting a strain showing improved productivity for L-glutamine from bacteria randomly incorporated with mutations, they require much labor and suffer from difficulties.
SUMMARY OF THE INVENTION
[0007] An object of the present invention is to find characteristics of coryneform bacteria providing improvement of L-glutamine productivity and suppression of by-production of L-glutamic acid, and thereby provide a method for producing L-glutamine utilizing a strain having such characteristics.
[0008] The inventors of the present invention assiduously studied in order to achieve the aforementioned object. As a result, they found that a strain of coryneform bacterium of which intracellular glutamine synthetase activity was enhanced showed more excellent L-glutamine producing ability and could markedly suppress the by-production of L-glutamic acid compared with strains showing the glutamine synthetase activity comparable to that of wild strains. Further, they found that production rate of L-glutamine was improved by simultaneously enhancing glutamine synthetase activity and glutamate dehydrogenase activity. Furthermore, they successfully isolated a novel gene coding for glutamine synthetase and a novel gene coding for glutamine synthetase adenylyl transferase, and thus accomplished the present invention.
[0009] That is, the present invention provides the followings.
[0010] (1) A coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced.
[0011] (2) The bacterium according to (1), wherein the glutamine synthetase activity is enhanced by increasing expression amount of a glutamine synthetase gene.
[0012] (3) The bacterium according to (2), wherein the expression amount of the glutamine synthetase gene is increased by increasing copy number of a gene coding for glutamine synthetase or modifying an expression control sequence of the gene so that expression of the gene coding for the intracellular glutamine synthetase of the bacterium should be enhanced.
[0013] (4) The bacterium according to (1), wherein the glutamine synthetase activity is enhanced by deficiency in activity control of intracellular glutamine synthetase by adenylylation.
[0014] (5) The bacterium according to (4), wherein the activity control of intracellular glutamine synthetase by adenylylation is defected by one or more of harboring glutamine synthetase of which activity control by adenylylation is defected, decrease of glutaimine synthetase adenylyl transferase activities in the bacterial cell and decrease of PII protein activity in the bacterial cell.
[0015] (6) The bacterium according to any one of (1) to (5), wherein the bacterium has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced.
[0016] (7) The bacterium according to (6), wherein the glutamate dehydrogenase activity is enhanced by increasing expression amount of a glutamate dehydrogenase gene.
[0017] (8) The bacterium according to (7), wherein the expression amount of the glutamate dehydrogenase gene is increased by increasing copy number of the gene coding for glutamate dehydrogenase or modifying an expression control sequence of the gene so that expression of the gene coding for the intracellular glutamate dehydrogenase of the bacterium should be increased.
[0018] (9) A method for producing L-glutamine, which comprises culturing a bacterium according to any one of (1) to (8) in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.
[0019] (10) A DNA coding for a protein defined in the following (A) or (B):
[0020] (A) a protein that has the amino acid sequence of SEQ ID NO: 2,
[0021] (B) a protein that has the amino acid sequence of SEQ ID NO: 2 including substitution, deletion, insertion, addition or inversion of one or several amino acid residues and has glutamine synthetase activity.
[0022] (11) The DNA according to (10), which is a DNA defined in the following (a) or (b):
[0023] (a) a DNA containing the nucleotide sequence of the nucleotide numbers 659-1996 in the nucleotide sequence of SEQ ID NO: 1,
[0024] (b) a DNA that is hybridizable with the nucleotide sequence of the nucleotide numbers 659-1996 in the nucleotide sequence of SEQ ID NO: 1 or a probe that can be prepared from the sequence under the stringent conditions and codes for a protein having glutamine synthetase activity.
[0025] (12) A DNA coding for a protein defined in the following (C) or (D):
[0026] (C) a protein that has the amino acid sequence of SEQ ID NO: 3,
[0027] (D) a protein that has the amino acid sequence of SEQ ID NO: 3 including substitution, deletion, insertion, addition or inversion of one or several amino acid residues and has glutamine synthetase adenylyl transferase activities.
[0028] (13) The DNA according to (12), which is a DNA defined in the following (c) or (d):
[0029] (c) a DNA containing the nucleotide sequence of nucleotide numbers 2006-5200 in the nucleotide sequence of SEQ ID NO: 1,
[0030] (d) a DNA that is hybridizable with the nucleotide sequence of the nucleotide numbers 2006-5200 in the nucleotide sequence of SEQ ID NO: 1 or a probe that can be prepared from the sequence under the stringent conditions and codes for a protein having glutamine synthetase adenylyl transferase activities.
[0031] According to the present invention, the by-production of L-glutamic acid can be suppressed and the production efficiency of L-glutamine can be improved in the production of L-glutamine by fermentation utilizing coryneform bacteria. Further, the DNA of the present invention can be used for breeding of L-glutamine producing coryneform bacteria.
PREFERRED EMBODIMENTS OF THE INVENTION
[0032] Hereafter, the present invention will be explained in detail.
[0033] (1) Coryneform bacteria of the present invention
[0034] In the present invention, “coryneform bacteria” include those having hitherto been classified into the genus Brevibacterium, but united into the genus Corynebacterium at present ( Int. J. Syst. Bacteriol., 41, 255 (1981)), and include bacteria belonging to the genus Brevibacterium closely relative to the genus Corynebacterium. Examples of such coryneform bacteria are mentioned below.
[0035] Corynebacterium acetoacidophilum
[0036] Corynebacterium acetoglutamicum
[0037] Corynebacterium alkanolyticum
[0038] Corynebacterium callunae
[0039] Corynebacterium glutamicum
[0040] Corynebacterium lilium
[0041] Corynebacterium melassecola
[0042] Corynebacterium thermoaminogenes
[0043] Corynebacterium herculis
[0044] Brevibacterium divaricatum
[0045] Brevibacterium flavum
[0046] Brevibacterium immariophilum
[0047] Brevibacterium lactofermentum
[0048] Brevibacterium roseum
[0049] Brevibacterium saccharolyticum
[0050] Brevibacterium thiogenitalis
[0051] Brevibacterium ammoniagenes
[0052] Brevibacterium album
[0053] Brevibacterium cerium
[0054] Microbacterium ammoniaphilum
[0055] Specifically, the following strains can be exemplified.
[0056] Corynebacterium acetoacidophilum ATCC 13870
[0057] Corynebacterium acetoglutamicum ATCC 15806
[0058] Corynebacterium alkanolyticum ATCC 21511
[0059] Corynebacterium callunae ATCC 15991
[0060] Corynebacterium glutamicum ATCC 13020, 13032, 13060
[0061] Corynebacterium lilium ATCC 15990
[0062] Corynebacterium melassecola ATCC 17965
[0063] Corynebacterium thermoaminogenes AJ12340 (FERM BP-1539)
[0064] Corynebacterium herculis ATCC 13868
[0065] Brevibacterium divaricatum ATCC 14020
[0066] Brevibacterium flavum ATCC 13826, ATCC 14067, AJ12418 (FERM BP-2205)
[0067] Brevibacterium immariophilum ATCC 14068
[0068] Brevibacterium lactofermentum ATCC 13869
[0069] Brevibacterium roseum ATCC 13825
[0070] Brevibacterium saccharolyticum ATCC 14066
[0071] Brevibacterium thiogenitalis ATCC 19240
[0072] Brevibacterium ammoniagenes ATCC 6871, ATCC 6872
[0073] Brevibacterium album ATCC 15111
[0074] Brevibacterium cerium ATCC 15112
[0075] Microbacterium ammoniaphilum ATCC 15354
[0076] To obtain these strains, one can be provided them from, for example, the American Type Culture Collection (10801 University Boulevard, Manassas, Va. 20110-2209, United States of America). That is, each strain is assigned its registration number, and one can request provision of each strain by utilizing its registration number. The registration numbers corresponding to the strains are indicated on the catalog of the American Type Culture Collection. Further, the AJ12340 strain was deposited on Oct. 27, 1987 at the National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry (currently, the independent administrative corporation, National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary (Chuo Dai-6, 1-1 Higashi 1-Chome, Tsukuba-shi, Ibaraki-ken, Japan, postal code: 305-8566)) as an international deposit under the provisions of the Budapest Treaty, and received an accession number of FERM BP-1539. The AJ12418 strain was deposited on Jan. 5, 1989 at the National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry as an international deposit under the provisions of the Budapest Treaty and received an accession number of FERM BP-2205.
[0077] In the present invention, “L-glutamine producing ability” means an ability to accumulate L-glutamine in a medium, when the coryneform bacterium of the present invention is cultured in the medium. This L-glutamine producing ability may be possessed by the bacterium as a property of a wild strain of coryneform bacteria or may be imparted or enhanced by breeding.
[0078] For imparting or enhancing the L-glutamine producing ability by breeding, there can be used the method of isolation of 6-diazo-5-oxo-norleucine resistant strain (Japanese Patent Laid-open Publication No. 3-232497), the method of isolation of purine analogue resistant and/or methionine sulfoxide resistant strain (Japanese Patent Laid-open Publication No. 61-202694), the method of isolation of α-ketomalonic acid resistant strain (Japanese Patent Laid-open Publication No. 56-151495), the method of imparting resistance to a peptide containing glutamic acid (Japanese Patent Laid-open Publication No. 2-186994) and so forth. As specific examples of coryneform bacteria having L-glutamine producing ability, the following strains can be mentioned. Brevibacterium flavum AJ11573 (FERM P-5492, refer to Japanese Patent Laid-open Publication No. 56-151495) Brevibacterium flavum AJ12210 (FERM P-8123, refer to Japanese Patent Laid-open Publication No. 61-202694) Brevibacterium flavum AJ12212 (FERM P-8123, refer to Japanese Patent Laid-open Publication No. 61-202694) Brevibacterium flavum AJ12418 (FERM-BP2205, refer to Japanese Patent Laid-open Publication No. 2-186994) Brevibacterium flavum DH18 (FERM P-11116, refer to Japanese Patent Laid-open Publication No. 3-232497) Corynebacterium melassecola DH344 (FERM P-11117, refer to Japanese Patent Laid-open Publication No. 3-232497) Corynebacterium glutamicum AJ11574 (FERM P-5493, refer to Japanese Patent Laid-open Publication No. No. 56 151495)
[0079] The term “modified so that intracellular glutamine synthetase (henceforth also referred to as “GS”) activity should be enhanced” means that the GS activity per cell has become higher than that of a non-modified strain, for example, a wild-type coryneform bacterium. For example, there can be mentioned a case where number of GS molecules per cell increases, a case where GS specific activity per GS molecule increases and so forth. Further, as a wild-type coryneform bacterium that serves as an object for comparison, for example, the Brevibacterium flavum ATCC 14067 can be mentioned. As a result of enhancement of intracellular GS activity, there are obtained an effect that the amount of L-glutamine accumulation in a medium increases, an effect that the by-production of L-glutamic acid decreases and so forth.
[0080] Enhancement of GS activity in a coryneform bacterium cell can be attained by enhancement of expression of a gene coding for GS. Increase of the expression amount of the gene can be attained by increasing copy number of the gene coding for GS. For example, a recombinant DNA can be prepared by ligating a gene fragment coding for GS with a vector functioning in the bacterium, preferably a multi-copy type vector, and introduced into a host having L-glutamine producing ability to transform it. Alternatively, the aforementioned recombinant DNA can be introduced into a wild-type coryneform bacterium to obtain a transformant, and then the transformant can be imparted with L-glutamine producing ability.
[0081] As the GS gene, any of genes derived from coryneform bacteria and genes derived from other organisms such as bacteria belonging to the genus Escherichia can be used. Among these, genes derived from coryneform bacteria are preferred in view of ease of expression.
[0082] As the gene coding for GS of coryneform bacteria, glnA has already been elucidated ( FEMS Microbiology Letters, 81-88, 154, 1997). Therefore, a GS gene can be obtained by PCR (polymerase chain reaction; refer to White, T. J. et al., Trends Genet., 5, 185 (1989)) utilizing primers prepared based on the nucleotide sequence of the gene, for example, the primers mentioned in Sequence Listing as SEQ ID NOS: 4 and 5, and chromosomal DNA of coryneform bacterium as a template. Genes coding for GS of other microorganisms can be obtained in a similar manner.
[0083] The chromosomal DNA can be prepared from a bacterium, which is a DNA donor, by the method of Saito and Miura (refer to H. Saito and K. Miura, Biochem. Biophys. Acta, 72, 619 (1963), Text for Bioengineering Experiments, Edited by the Society for Bioscience and Bioengineering, Japan, pp.97-98, Baifukan, 1992), for example.
[0084] Incidentally, an isozyme often exists for an enzyme involved in an amino acid biosynthesis system. The inventors of the present invention successfully isolated and cloned a gene coding for an isozyme of GS of coryneform bacteria by utilizing homology with respect to the nucleotide sequence of the aforementioned g1nA gene. This gene is referred to as “g1nA2”. The process for obtaining it will be described later. g1nA2 as well as g1nA can be used for enhancement of the GS activity of coryneform bacteria.
[0085] If the GS gene amplified by the PCR method is ligated to a vector DNA autonomously replicable in a cell of Escherichia coli and/or coryneform bacteria to prepare a recombinant DNA and this is introduced into Escherichia coli , subsequent procedures become easy. Examples of the vector autonomously replicable in a cell of Escherichia coil include pUC19, pUC18, pHSG299, pHSG399, pHSG398, RSF1010, pBR322, pACYC184, pMW219 and so forth.
[0086] A vector that functions in coryneform bacteria means, for example, a plasmid that can autonomously replicate in coryneform bacteria. Specific examples thereof include the followings. pAM330 (refer to Japanese Patent Laid-open Publication No. 58-67699) pHM1519 (refer to Japanese Patent Laid-open Publication No. 58-77895)
[0087] Moreover, if a DNA fragment having an ability to make a plasmid autonomously replicable in coryneform bacteria is taken out from these vectors and inserted into the aforementioned vectors for Escherichia coli , they can be used as a so-called shuttle vector autonomously replicable in both of Escherichia coli and coryneform bacteria.
[0088] Examples of such a shuttle vector include those mentioned below. There are also indicated microorganisms that harbor each vector, and accession numbers thereof at the international depositories are shown in the parentheses, respectively.
[0089] pAJ655 Escherichia coli AJ11882 (FERM BP-136) Corynebacterium glutamicum SR8201 (ATCC 39135)
[0090] pAJ1844 Escherichia coli AJ11883 (FERM BP-137) Corynebacterium glutamicum SR8202 (ATCC 39136)
[0091] pAJ611 Escherichia coli AJ11884 (FERM BP-138)
[0092] pAJ3148 Corynebacterium glutamicum SR8203 (ATCC 39137)
[0093] pAJ440 Bacillus subtilis AJ11901 (FERM BP-140)
[0094] pHC4 Escherichia coli AJ12617 (FERM BP-3532)
[0095] These vectors can be obtained from the deposited microorganisms as follows. That is, microbial cells collected in their exponential growth phase are lysed by using lysozyme and SDS, and centrifuged at 30000×g. The supernatant obtained from the lysate is added with polyethylene glycol, fractionated and purified by cesium chloride-ethidium bromide equilibrium density gradient centrifugation.
[0096] In order to prepare a recombinant DNA by ligating a GS gene and a vector that can function in a cell of coryneform bacterium, a vector is digested with a restriction enzyme corresponding to the terminus of the gene containing the GS gene. Ligation is usually performed by using a ligase such as T4 DNA ligase.
[0097] To introduce the recombinant DNA prepared as described above into a microorganism, any known transformation methods that have hitherto been reported can be employed. For instance, employable are a method of treating recipient cells with calcium chloride so as to increase the permeability of DNA, which has been reported for Escherichia coli K-12 (Mandel, M. and Higa, A., J. Mol. Biol., 53, 159 (1970)), and a method of preparing competent cells from cells which are at the growth phase followed by introducing the DNA thereinto, which has been reported for Bacillus subtilis (Duncan, C. H., Wilson, G. A. and Young, F. E., Gene, 1, 153 (1977)). In addition to these, also employable is a method of making DNA-recipient cells into protoplasts or spheroplasts, which can easily take up recombinant DNA, followed by introducing the recombinant DNA into the cells, which is known to be applicable to Bacillus subtilis , actinomycetes and yeasts (Chang, S. and Choen, S. N., Molec. Gen. Genet., 168, 111 (1979); Bibb, M. J., Ward, J. M. and Hopwood, O. A., Nature, 274, 398 (1978); Hinnen, A., Hicks, J. B. and Fink, G. R., Proc. Natl. Sci. USA, 75, 1929 (1978)). The transformation of coryneform bacteria can also be performed by the electric pulse method (Sugimoto et al., Japanese Patent Laid-open No. 2-207791).
[0098] Increase of copy number of GS gene can also be achieved by introducing multiple copies of the GS gene into chromosomal DNA of coryneform bacteria. In order to introduce multiple copies of the GS gene into chromosomal DNA of coryneform bacteria, homologous recombination is carried out by using a sequence whose multiple copies exist in the chromosomal DNA as targets. As sequences whose multiple copies exist in the chromosomal DNA, repetitive DNA, inverted repeats existing at the end of a transposable element can be used. Also, as disclosed in Japanese Patent Laid-open No. 2-109985, it is possible to incorporate the GS gene into transposon, and allow it to be transferred to introduce multiple copies of the gene into the chromosomal DNA.
[0099] Enhancement of the GS activity can also be attained by, besides being based on the aforementioned gene amplification, replacing an expression control sequence of the GS gene on chromosomal DNA or plasmid, such as a promoter, with a stronger one. For example, lac promoter, trp promoter, trc promoter and so forth are known as strong promoters. Moreover, it is also possible to introduce nucleotide substitution for several nucleotides into a promoter region for the GS gene so that it should be modified into a stronger one, as disclosed in International Patent Publication WO00/18935. By such substitution or modification of promoter, expression of the GS gene is enhanced and thus GS activity is enhanced. Such modification of expression control sequence may be combined with the increase of copy number of the GS gene.
[0100] The substitution of expression control sequence can be performed, for example, in the same manner as the gene substitution using a temperature sensitive plasmid described later. Examples of the temperature sensitive plasmid of coryneform bacteria include p48K, pSFKT2 (refer to Japanese Patent Laid-open Publication No. 2000-262288 for the both), pHSC4 (refer to France Patent Laid-open Publication No. 2667875, 1992 and Japanese Patent Laid-open Publication No. 5-7491) and so forth. These plasmids can at least autonomously replicate at a temperature of 25° C., but cannot autonomously replicate at a temperature of 37° C. in coryneform bacteria. Although pSFKT2 was used for the substitution for the promoter sequence of the GDH gene in the example mentioned later, gene substitution can be performed in a similar manner by using pHSC4 instead of pSFKT2. Escherichia coli AJ12571 harboring pHSC4 was deposited on Oct. 11, 1990 at the National Institute of Bioscience and Human-Technology, Agency of Industrial Science and Technology, Ministry of International Trade and Industry (currently, the independent administrative corporation, National Institute of Advanced Industrial Science and Technology, International Patent Organism Depositary (Chuo Dai-6, 1-1 Higashi 1-Chome, Tsukuba-shi, Ibaraki-ken, Japan, postal code: 305-8566)), and received an accession number of FERM P-11763. Then, it was transferred to an international deposit under the provisions of the Budapest Treaty on Aug. 26, 1991, and received an accession number of FERM BP-3524.
[0101] Enhancement of the GS activity can be attained also by deficiency in regulation by the adenylylation of intracellular GS, besides based on the increase of expression amount of the GS gene described above. GS changes into an inactive form by adenylylation of a tyrosine residue in the amino acid sequence ( Proc. Natl. Acad. Sci. USA, 642-649, (58) 1967; J. Biol. Chem., 3769-3771, (243) 1968). Therefore, by defect of this adenylylation of GS, the intracellular GS activity can be enhanced. The defect of adenylylation used herein means not only substantially complete deregulation by the adenylylation but also such reduction of the adenylylation that the intracellular GS activity should be enhanced.
[0102] The adenylylation of GS is generally performed by adenylyl transferase ( Proc. Natl. Acad. Sci. USA, 1703-1710, (58) 1967). It has been suggested that, in coryneform bacteria, the 405th tyrosine residue of the glnA gene product, which is represented by the sequence of Genebank accession Y13221, is adenylylated (FEMS Microbiology Letters, 303-310, 1999 (173)). This inactivation by the adenylylation of GS can be defected by introducing a mutation into the glnA gene so that the tyrosine residue should be replaced with another amino acid residue.
[0103] Further, the inactivation of GS by the adenylylation can also be defected by reducing the activities of intracellular glutamine synthetase adenylyl transferase (ATase). Although adenylyl transferase of coryneform bacteria had been unknown, the inventors of the present invention successfully isolated a gene coding for adenylyl transferase of coryneform bacteria, glnE. The process therefor will be described later.
[0104] To reduce the intracellular ATase activity of coryneform bacteria, there can be used, for example, a method of treating the coryneform bacteria by ultraviolet irradiation or with a mutagenizing agent used for usual mutagenesis treatment such as N-methyl-N′-nitro-N-nitrosoguanidine (NTG) or a nitrous acid and selecting a mutant strain in which the ATase activity is reduced. Coryneform bacteria having reduced ATase activity can also be obtained by gene disruption, besides the mutagenesis treatment. That is, a coryneform bacterium can be transformed with a DNA containing a glnE gene modified with deletion of partial sequence of the gene coding for ATase so as not to produce ATase functioning normally (deletion type glnE gene), so that recombination between the deletion type glnE gene and the glnE gene on the chromosome should occur to disrupt the glnE gene on the chromosome. Such gene disruption by gene substitution utilizing homologous recombination has already been established, and there are methods utilizing a linear DNA, a plasmid that contains a temperature sensitive replication origin and so forth.
[0105] A glnE gene on host chromosome can be replaced with the deletion type glnE gene, for example, as follows. That is, a recombinant DNA is prepared by inserting a temperature sensitive replication origin, a mutant glnE gene and a marker gene for resistance to a drug such as chloramphenicol, and a coryneform bacterium is transformed with the recombinant DNA. Further, the transformant is cultured at a temperature at which the temperature sensitive replication origin does not function, and then the transformant strain can be cultured in a medium containing the drug to obtain a transformant strain in which the recombinant DNA is incorporated into the chromosomal DNA.
[0106] In such a strain in which recombinant DNA is incorporated into chromosomal DNA as described above, the mutant glnE gene is recombined with the glnE gene originally present on the chromosome, and the two fusion genes of the chromosomal glnE gene and the deletion type glnE gene are inserted into the chromosome so that the other portions of the recombinant DNA (vector segment, temperature sensitive replication origin and drug resistance marker) should be present between the two fusion genes. Therefore, the transformant strain expresses normal ATase, because the normal glnE gene is dominant in this state.
[0107] Then, in order to leave only the deletion type glnE gene on the chromosomal DNA, one copy of the glnE gene is eliminated together with the vector segment (including the temperature sensitive replication origin and the drug resistance marker) from the chromosomal DNA by recombination of two of the glnE genes. In this case, the normal glne gene is left on the chromosomal DNA, and the deletion type glnE gene is excised from the chromosomal DNA, or to the contrary, the deletion type glnE gene is left on the chromosomal DNA, and the normal glnE gene is excised from the chromosome DNA. In the both cases, the excised DNA may be retained in the cell as a plasmid when the cell is cultured at a temperature at which the temperature sensitive replication origin can function. Subsequently, if the cell is cultured at a temperature at which the temperature sensitive replication origin cannot function, the glnE gene on the plasmid is eliminated together with the plasmid from the cell. Then, a strain in which glnE gene is disrupted can be obtained by selecting a strain in which the deletion type glnE gene is left on the chromosome using PCR, Southern hybridization or the like.
[0108] Further, the inactivation of GS by the adenylylation can also be canceled by reducing the intracellular activity of PII protein. It is known that the PII protein is also involved in the adenylylation of GS by ATase. The PII protein is a signal transfer protein for controlling the GS activity, and it is known to be uridylylated by uridylyl transferase (UTase). The uridylylated PII protein promotes deadenylylation of GS by ATase, and the deuridylylated PII protein promotes the adenylylation of GS by ATase.
[0109] It is reported that GS is highly adenylylated in a UTase deficient strain ( J. Bacteriology, 569-577, (134) 1978). This phenotype of excessive adenylylation is suppressed by mutation of the PII protein ( J. Bacteriology, 816-822, (164) 1985). That is, the inactivation of GS by the adenylylation can also be defected by reduction of PII protein activity. The reduction of PII protein activity means reduction of the function for promoting the adenylylation by ATase. The glnB gene coding for the PII protein of coryneform bacteria has been already isolated, and it is suggested that the suppression of GS by the adenylylation of GS is defected by deletion of the gene ( FEMS Microbiology Letters, 303-310, (173) 1999).
[0110] To reduce the PII protein activity of coryneform bacteria, there can be used, for example, a method of treating the coryneform bacteria by ultraviolet irradiation or with a mutagenizing agent used for usual mutagenesis treatment such as N-methyl-N′-nitro-N-nitrosoguanidine (NTG) or a nitrous acid and selecting a mutant strain in which the activity of PII protein is reduced. Coryneform bacteria having reduced PII protein activity can also be obtained by gene disruption, besides the mutagenesis treatment. That is, a coryneform bacterium can be transformed with DNA containing a glnB gene modified with deletion of partial sequence of the gene coding for PII protein so as not to produce PII protein functioning normally (deletion type glnB gene), so that recombination between the deletion type glnB gene and the glnB gene on the chromosome should occur to disrupt the glnB gene on the chromosome. Such gene destruction by utilizing homologous recombination has already been established, and there are methods utilizing a linear DNA, a plasmid that contains a temperature sensitive replication origin and so forth.
[0111] A glnB gene on host chromosome can be replaced with the deletion type glnB gene, for example, as follows. That is, a recombinant DNA is prepared by inserting a temperature sensitive replication origin, a mutant glnB gene and a marker gene for resistance to a drug such as chloramphenicol, and a coryneform bacterium is transformed with the recombinant DNA. Further, the resultant transformant strain is cultured at a temperature at which the temperature sensitive replication origin does not function, and then the transformant strain can be cultured in a medium containing the drug to obtain a transformant strain in which the recombinant DNA is incorporated into the chromosomal DNA.
[0112] In such a strain in which recombinant DNA is incorporated into chromosomal DNA as described above, the mutant glnB gene is recombined with the glnB gene originally present on the chromosome, and the two fusion genes of the chromosomal glnB gene and the deletion type glnB gene are inserted into the chromosome so that the other portions of the recombinant DNA (vector segment, temperature sensitive replication origin and drug resistance marker) should be present between the two fusion genes. Therefore, the transformant expresses normal PII protein, because the normal glnB gene is dominant in this state.
[0113] Then, in order to leave only the deletion type glnB gene on the chromosomal DNA, one copy of the glnB gene is eliminated together with the vector segment (including the temperature sensitive replication origin and the drug resistance marker) from the chromosomal DNA by recombination of two of the glnB genes. In this case, the normal glnB gene is left on the chromosomal DNA, and the deletion type glnB gene is excised from the chromosomal DNA, or to the contrary, the deletion type glnB gene is left on the chromosomal DNA, and the normal glnB gene is excised from the chromosome DNA. In the both cases, the excised DNA may be stably retained in the cell as a plasmid when the cell is cultured at a temperature at which the temperature sensitive replication origin can function. Subsequently, if the cell is cultured at a temperature at which the temperature sensitive replication origin does not function, the glnB gene on the plasmid is eliminated together with the plasmid from the cell. Then, a strain in which glnB gene is disrupted can be obtained by selecting a strain in which the deletion type glnB gene is left on the chromosome using PCR, Southern hybridization or the like.
[0114] Elimination of the adenylylation of GS can also be attained by a combination of two or three items of such mutation of GS that should eliminate the aforementioned adenylylation, reduction of the ATase activity and reduction of the PII protein activity.
[0115] Although enhancement of the GS activity can also be realized by elimination of the adenylylation of GS by ATase, it may also be attained by a combination of it with the aforementioned means for increasing copy number of the GS gene or means for modifying an expression control sequence.
[0116] In order to efficiently produce L-glutamine by using the coryneform bacterium of the present invention, it is preferable to use a strain that has enhanced glutamate dehydrogenase (henceforth also referred to as “GDH”) activity concurrently with the enhanced GS activity.
[0117] The term “modified so that intracellular GDH activity should be enhanced” means that the GDH activity per cell has become higher than that of a non-modified strain, for example, a wild-type coryneform bacterium. For example, there can be mentioned a case where number of GDH molecules per cell increases, a case where GDH specific activity per GDH molecule increases and so forth. Further, as a wild-type coryneform bacterium that serves as an object for comparison, for example, the Brevibacterium flavum ATCC 14067 can be mentioned. As a result of enhancement of intracellular GDH activity, there are obtained an effect that culture time of a coryneform bacterium having L-glutamine producing ability is shortened.
[0118] Enhancement of the GDH activity in a coryneform bacterium cell can be attained by enhancement of expression of a gene coding for GDH. Enhancement of the expression amount of the gene can be attained by increasing copy number of the gene coding for GDH. For example, a recombinant DNA can be prepared by ligating a gene fragment coding for GDH with a vector functioning in the bacterium, preferably a multi-copy type vector, and introduced into a host having L-glutamine producing ability to transform it. Alternatively, the aforementioned recombinant DNA can be introduced into a wild-type coryneform bacterium to obtain a transformant strain, and then the obtained transformant strain can be imparted with L-glutamine producing ability.
[0119] As the gene coding for GDH, any of genes derived from coryneform bacteria and genes derived from other organisms such as bacteria belonging to the genus Escherichia can be used. Among these, genes derived from coryneform bacteria are preferred in view of ease of expression.
[0120] Nucleotide sequence of a gene coding for GDH (gdh gene) of coryneform bacteria has already been elucidated ( Molecular Microbiology, 6 (3), 317-326 (1992)). Therefore, a GDH gene can be obtained by PCR utilizing primers prepared based on the nucleotide sequence, for example, the primers mentioned in Sequence Listing as SEQ ID NOS: 12 and 13, and chromosomal DNA of coryneform bacterium as a template. Genes coding for GDH of microorganisms other than coryneform bacteria can also be obtained in a similar manner.
[0121] The gdh gene can be introduced into coryneform bacteria in a manner similar to that used for the aforementioned GS gene.
[0122] In the coryneform bacterium of the present invention, activities of enzymes other than GS and GDH catalyzing reactions of the L-glutamine biosynthesis may be enhanced. Examples of the enzymes catalyzing reactions of the L-glutamine biosynthesis include isocitrate dehydrogenase, aconitate hydratase, citrate synthase, pyruvate dehydrogenase, phosphoenolpyruvate carboxylase, pyruvate carboxylase, pyruvate kinase, phosphofructokinase and so forth.
[0123] Further, activities of enzymes that catalyze reactions branching off from the L-glutamine biosynthesis pathway and producing compounds other than L-glutamine may be reduced or eliminated. Examples of the enzymes catalyzing such reactions include isocitrate lyase, α-ketoglutarate dehydrogenase, glutamate synthase and so forth.
[0124] (2) Production of L-glutamine using microorganism of the present invention
[0125] By culturing a coryneform bacterium obtained as described above in a medium to produce and accumulate L-glutamine in the medium and correcting the L-glutamine from the medium, L-glutamine can be efficiently produced and the by-production of L-glutamic acid can be suppressed.
[0126] In order to produce L-glutamine by using the coryneform bacterium of the present invention, culture can be performed in a conventional manner using a usual medium containing a carbon source, nitrogen source and mineral salts as well as organic trace nutrients such as amino acids and vitamins, as required. Either a synthetic medium or a natural medium may be used. Any kinds of carbon source and nitrogen source may be used so long as they can be utilized by a strain to be cultured.
[0127] As the carbon source, there are used sugars such as glucose, glycerol, fructose, sucrose, maltose, mannose, galactose, starch hydrolysate and molasses, and organic acids such as acetic acid and citric acid, and alcohols such as ethanol can also be used each alone or in a combination with other carbon sources.
[0128] As the nitrogen source, there are used ammonia, ammonium salts such as ammonium sulfate, ammonium carbonate, ammonium chloride, ammonium phosphate and ammonium acetate, nitrate salts and so forth.
[0129] As the organic trace nutrients, amino acids, vitamins, fatty acids, nucleic acids, those containing those substances such as peptone, casamino acid, yeast extract and soybean protein decomposition product and so forth are used. When an auxotrophic mutant that requires an amino acid or the like for its growth is used, it is preferable to supplement the required nutrient.
[0130] As the mineral salts, phosphates, magnesium salts, calcium salts, iron salts, manganese salts and so forth are used.
[0131] The culture is performed as aeration culture, while the fermentation temperature is controlled to be 20-45° C., and pH to be 5-9. When pH falls during the culture, the medium is neutralized by addition of calcium carbonate or with an alkali such as ammonia gas. A substantial amount of L-glutamine is accumulated in the culture broth after 10 hours to 120 hours of culture in such a manner as described above.
[0132] Collection of L-glutamine from the culture broth after the culture may be performed in a conventional manner. For example, after the cells were removed from the culture broth, L-glutamine can be collected by concentrating the broth to crystallize L-glutamine.
[0133] (3) DNA coding for protein having glutamine synthetase activity (glnA2 gene) and DNA coding for protein having glutamine synthetase and adenylyl transferase activities (glnE gene) according to the present invention
[0134] The first DNA of the present invention is a gene coding for GS. The second DNA of the present invention is a gene coding for ATase. These genes can be obtained from a chromosome DNA library of Brevibacterium lactofermentum by hybridization using a partial fragment of a known glnA gene as a probe. The partial fragment of a known glnA gene can be obtained by PCR amplification using chromosome DNA of Brevibacterium lactofermentum , for example, Brevibacterium lactofermentum ATCC 13869 strain, as a template and the primers shown as SEQ ID NOS: 18 and 19.
[0135] Methods of production of genomic DNA library, hybridization, PCR, preparation of plasmid DNA, digestion and ligation of DNA, transformation and so forth for obtaining the DNA of the present invention and enhancement of the GS activity and GDH activity are described in Sambrook, J., Fritsh, E. F. and Maniatis, T., “Molecular Cloning”, Cold Spring Harbor Laboratory Press, 1.21, 1989.
[0136] The nucleotide sequences of the aforementioned primers were designed based on the nucleotide sequence of a glnA gene of Corynebacterium glutamicum (GenBank accession Y13221). By using these primers, a DNA fragment containing a region corresponding to the nucleotide numbers 1921-2282 of the glnA gene (GenBank accession Y13221) can be obtained.
[0137] Examples of nucleotide sequence of DNA fragment containing glnA2 according to the present invention, which is obtained as described above, and amino acid sequence that can be encoded by the sequence are shown as SEQ ID NO: 1. Further, only an amino acid sequence of protein having glutamine synthetase activity, which is encoded by glnA2, is shown in SEQ ID NO: 2.
[0138] Further, in the aforementioned DNA fragment, another ORF was found immediately downstream from ORF of the glnA2 gene. Based on homology comparison with respect to known sequences, that ORF was expected to be a gene (glnE) coding for a protein having glutamine synthetase adenylyl transferase activities (ATase). Only the amino acid sequence of the protein having the ATase activity is shown as SEQ ID NO: 3.
[0139] Nucleotide sequences of the DNA fragments containing glnA2 or glnE according to the present invention were clarified by the present invention. Therefore, they can be isolated from chromosomal DNA of Brevibacterium lactofermentum by the PCR method using primers produced based on the nucleotide sequences.
[0140] The first DNA of the present invention may be one coding for glutamine synthetase including substitution, deletion, insertion, addition or inversion of one or several amino acids at one or more sites, so long as the glutamine synthetase activity of the encoded protein is not defected. Although the number of “several” amino acids referred to herein differs depending on position or type of amino acid residues in the three-dimensional structure of the protein, it may be specifically 2 to 90, preferably 2 to 50, more preferably 2 to 20.
[0141] The second DNA of the present invention may be one coding for glutamine synthetase adenylyl transferase including substitution, deletion, insertion, addition or inversion of one or several amino acids at one or more sites, so long as the glutamine synthetase adenylyl transferase activities of the encoded protein are not defected. Although the number of “several” amino acids referred to herein differs depending on position or type of amino acid residues in the three-dimensional structure of the protein, it may be specifically 2 to 350, preferably 2 to 50, more preferably 2 to 20. Even in a case that the glutamine synthetase and adenylyl transferase activities are impaired, such a DNA fall within the scope of the present invention so long as it causes homologous recombination.
[0142] A DNA coding for the substantially same protein as the aforementioned GS or ATase can be obtained by, for example, modifying the nucleotide sequence of glnA2 or glnE by means of the site-directed mutagenesis method so that one or more amino acid residues at a specified site should involve substitution, deletion, insertion, addition or inversion. A DNA modified as described above may also be obtained by a conventionally known mutagenesis treatment. The mutagenesis treatment includes a method of treating a DNA before the mutagenesis treatment in vitro with hydroxylamine or the like, and a method for treating a microorganism such as an genus Escherichia harboring a DNA before the mutagenesis treatment by ultraviolet irradiation or with a mutagenizing agent used for a usual mutagenesis treatment such as N-methyl-N′-nitro-N-nitrosoguanidine (NTG) and nitrous acid.
[0143] A DNA coding for substantially the same protein as glutamine synthetase or glutamine synthetase adenylyl transferase can be obtained by expressing a DNA having such a mutation as described above in an appropriate cell, and investigating activity of an expressed product. A DNA coding for substantially the same protein as GS or ATase can also be obtained by isolating a DNA that is hybridizable with a probe having a nucleotide sequence comprising, for example, the nucleotide sequence corresponding to nucleotide numbers of 659 to 1996 or 2066 to 5200 of the nucleotide sequence shown in Sequence Listing as SEQ ID NO: 1, under the stringent conditions, and codes for a protein having the glutamine synthetase or a protein having the glutamine synthetase adenylyl transferase activity, from DNA coding for glutamine synthetase or glutamine synthetase and adenylyl transferase having a mutation or from a cell harboring it. The “stringent conditions” referred to herein is a condition under which so-called specific hybrid is formed, and non-specific hybrid is not formed. It is difficult to clearly express this condition by using any numerical value. However, for example, the stringent conditions are exemplified by a condition under which DNAs having high homology, for example, DNAs having homology of not less than 50% are hybridized with each other, but DNAs having homology lower than the above are not hybridized with each other. Alternatively, the stringent conditions are exemplified by a condition under which DNAs are hybridized with each other at a salt concentration corresponding to an ordinary condition of washing in Southern hybridization, i.e., 1×SSC, 0.1% SDS, preferably 0.1×SSC, 0.1% SDS, at 60° C.
[0144] As a probe, a partial sequence of the nucleotide sequence of SEQ ID NO: 1 can also be used. Such a probe may be prepared by PCR using oligonucleotides produced based on the nucleotide sequence of SEQ ID NO: 1 as primers, and a DNA fragment containing the nucleotide sequence of SEQ ID NO: 1 as a template. When a DNA fragment in a length of about 300 bp is used as the probe, the conditions of washing for the hybridization consist of, for example, 50° C., 2×SSC and 0.1% SDS.
[0145] Genes that are hybridizable under such conditions as described above includes those having a stop codon in the genes, and those having no activity due to mutation of active center. However, such mutation can be easily removed by ligating each gene with a commercially available activity expression vector, and measuring the glutamine synthetase or glutamine synthetase adenylyl transferase activities. The glutamine synthetase activity can be measured by, for example, the method described in Methods in Enzymology , Vol. XVIIA, 910-915, ACADEMIC PRESS (1970), and the glutamine synthetase adenylyl transferase activities can be measured by, for example, the method described in Methods in Enzymology , Vol. XVIIA, 922-923, ACADEMIC PRESS (1970). Even a DNA coding for glutamine synthetase adenylyl transferase of which activities are reduced or deleted can also be used in the present invention.
[0146] Specific examples of the DNA coding for a protein substantially the same as GS include DNA coding for a protein that has homology of preferably 80% or more, more preferably 85% or more, still more preferably 90% or more, with respect to the amino acid sequence shown as SEQ ID NO: 2 and has GS activity. Specific examples of the DNA coding for a protein substantially the same as ATase include DNA coding for a protein that has homology of preferably 65% or more, more preferably 80% or more, still more preferably 90% or more, with respect to the amino acid sequence shown as SEQ ID NO: 3 and has ATase activity.
BEST MODE FOR CARRYING OUR THE INVENTION
[0147] Hereafter, the present invention will be explained more specifically with reference to the following examples.



EXAMPLE 1
Evaluation of GS Gene-Amplified Strain
[0148] (1) Cloning of glnA gene of coryneform bacterium
[0149] The glnA sequence of Corynebacterium glutamicum had been already clarified ( FEMS Microbiology Letters, 81-88, (154) 1997). Based on the reported nucleotide sequence, the primers shown in Sequence Listing as SEQ ID NOS: 4 and 5 were synthesized, and a glnA fragment was amplified by the PCR method using chromosome DNA of Brevibacterium flavum ATCC 14067 strain as a template.
[0150] The chromosomal DNA of Brevibacterium flavum ATCC 14067 strain was prepared by using Bacterial Genome DNA Purification Kit (Advanced Genetic Technologies Corp.). PCR was performed for 30 cycles each consisting of reactions at 94° C. for 30 seconds for denaturation, at 55° C. for 15 seconds for annealing and 72° C. for 2 minutes for extension by using Pyrobest DNA Polymerase (Takara Shuzo).
[0151] The produced PCR product was purified in a conventional manner, digested with a restriction enzyme SalI, ligated with pMW219 (Nippon Gene) digested with SalI by using a ligation kit (Takara Shuzo), and used to transform competent cells of Escherichia coli JM109 (Takara Shuzo). The cells were plated on L medium containing 10 μg/ml of IPTG, 40 μg/ml of X-Gal and 25 μg/ml of kanamycin and cultured overnight. Then, the appeared white colonies were picked up and separated into single colonies to obtain transformants.
[0152] Plasmids are prepared from the transformants by the alkali method, and a plasmid in which the glnA gene was inserted into the vector was designated as pMW219GS.
[0153] (2) Construction of plasmid having glnA and replication origin of coryneform bacteria
[0154] Further, in order to construct a plasmid having the glnA gene and a replication origin of coryneform bacteria, the plasmid pHK4 (refer to Japanese Patent Laid-open Publication No. 5-7491) containing replication origin of the plasmid pHM1519 ( Agric. Biol. Chem., 48, 2901-2903 (1984)) that had been already obtained and was autonomously replicable in coryneform bacteria was digested with restriction enzymes BamHI and KpnI to obtain a gene fragment containing the replication origin. The obtained fragment was blunt-ended by using DNA Blunt-ending Kit (Takara Shuzo) and inserted into the KpnI site of pMW219GS using a KpnI linker (Takara Shuzo). This plasmid was designated as pGS.
[0155] (3) Introduction of pGS into coryneform bacterium and evaluation of culture
[0156] An L-glutamine producing bacterium, Brevibacterium flavum AJ12418 (FERM BP-2205: refer to Japanese Patent Laid-open Publication No. 2-186994), was transformed with the plasmid pGS by the electric pulse method (refer to Japanese Patent Laid-open Publication No. 2-207791) to obtain a transformant. By using the obtained transformant AJ12418/pGS, culture for L-glutamine production was performed as follows.
[0157] Cells of AJ12418/pGS strain obtained by culture on a CM2B plate medium containing 25 μg/ml of kanamycin were inoculated into a medium containing 100 g of glucose, 60 g of (NH 4 ) 2 SO 4 , 2.5 g of KH 2 PO 4 , 0.4 g of MgSO 4 .7H 2 O, 0.01 g of FeSO 4 .7H 2 O, 350 μg of VB 1 -HCl, 4 μg of biotin, 200 mg of soybean hydrolysates and 50 g of CaCO 3 in 1 L of pure water (adjusted to pH 6.8 with NaOH) and cultured at 31.5° C. with shaking until the sugar in the medium was consumed.
[0158] After the completion of the culture, the amount of accumulated L-glutamine in the culture broth was analyzed by liquid chromatography for appropriately diluted culture broth. CAPCELL PAK C18 (Shiseido) was used as a column, and the sample was eluted with an eluent containing 0.095% phosphoric acid, 3.3 mM heptanesulfonic acid and 5% acetonitrile in 1 L of distilled water. The accumulated L-glutamine amount was analyzed based on variation of absorbance at 210 nm. The results of this analysis are shown in Table 1.
TABLE 1 Culture time Strain L-Gln (g/L) L-Glu (g/L) (hr) AJ12418 38.4 0.7 70 AJ12418/pGS 45.1 0.02 82

[0159] In the pGS-introduced strain, accumulation of L-glutamine (L-Gln) was markedly improved, and by-production of L-glutamic acid (L-Glu) was considerably suppressed. From these results, it was demonstrated that enhancement of GS was effective for improvement of yield in the production of L-glutamine. The data for the enzymatic activity of GS are shown in Table 2 of Example 2.
EXAMPLE 2
Evaluation of GS Adenylylation Site-Modified Strain
[0160] (1) Construction of adenylylation site-modified plasmid
[0161] The adenylylation site of glnA gene product of coryneform bacteria had been already clarified ( FEMS Microbiology Letters, 303-310, (173) 1999). Therefore, an adenylylation site-modified strain was obtained by replacing the glnA gene on the chromosome with a glnA gene of which adenylylation site was modified. Specific procedures will be described below.
[0162] First, PCR was performed by using chromosome DNA of Brevibacterium flavum ATCC 14067 strain as a template and the synthetic DNAs shown in Sequence Listing as SEQ ID NOS: 6 and 7 as primers to obtain an amplification product for the N-terminus side of the glnA gene. Separately, in order to obtain an amplification product for the C-terminus side of the glnA gene, PCR was performed by using chromosome DNA of Brevibacterium flavum ATCC 14067 strain as a template and the synthetic DNAs shown in Sequence Listing as SEQ ID NOS: 8 and 9 as primers. Since mismatches were introduced into the sequences shown in Sequence Listing as SEQ ID NOS: 7 and 8, a mutation was introduced into the terminal portion of each of the amplification products. Then, in order to obtain a glnA gene fragment introduced with a mutation, PCR was performed by using the aforementioned gene products for N- and C-terminus sides of glnA mixed in equimolar amounts as a template and the synthetic DNAs shown in Sequence Listing as SEQ ID NOS: 10 and 11 as primers to obtain a glnA gene amplification product introduced with a mutation at the adenylylation site. The produced PCR product was purified in a conventional manner, digested with HincII and inserted into the HincII site of pHSG299 (Takara Shuzo). This plasmid was designated as pGSA.
[0163] (2) Construction of adenylylation site-modified strain and evaluation of culture
[0164] Since the above pGSA does not contain a region that enables its autonomous replication within cells of coryneform bacteria, when a coryneform bacterium is transformed with this plasmid, a strain in which the plasmid is incorporated into chromosome by homologous recombination is obtained as a transformant although it occurs at an extremely low frequency.
[0165] The L-glutamine producing bacterium, Brevibacterium flavum AJ12418, was transformed with the plasmid pGSA at a high concentration by the electric pulse method (refer to Japanese Patent Laid-open Publication No. 2-207791), and transformants were obtained by using kanamycin resistance as a marker. Then, these transformants were subcultured and strains that became kanamycin sensitive were obtained. Further, the sequences of glnA gene of the kanamycin sensitive strains were determined, and a strain in which the adenylylation site in the sequence was replaced with that region of glnA derived from pGSA was designated as QA-1. Culture for L-glutamine production was performed in the same manner as described in Example 1, (3) using AJ12418, AJ12418/pGS and QA-1 strains. The results are shown in Table 2.
TABLE 2 GS activity Culture time Strain L-Gln (g/L) (U/mg) (hr) AJ12418 39.0 0.030 70 AJ12418/pGS 46.1 0.067 81 QA-1 44.3 0.040 72

[0166] For the QA-1 strain, improvement of L-glutamine accumulation was observed compared with AJ12418.
[0167] The results for measurement of GS activity of these strains are also shown in Table 2. The GS activity was measured by adding a crude enzyme solution to a solution containing 100 mM imidazole-HCl (pH 7.0), 0.1 mM NH 4 Cl, 1 mM MnCl 2 , 1 mM phosphoenolpyruvic acid, 0.3 mM NADH, 10 U of lactate dehydrogenase, 25 U of pyruvate kinase, 1 mM ATP and 10 mM MSG and measuring variation of absorbance at 340 nm at 30° C. referring to the method described in Journal of Fermentation and Bioengineering , Vol. 70, No. 3, 182-184, 1990. For the measurement of blank, the aforementioned reaction solution not containing MSG was used. The crude enzyme solution was prepared by separating cells from the aforementioned culture broth by centrifugation, washing the cells with 100 mM imidazole-HCl (pH 7.0), sonicating the cells and removing undisrupted cells and unsoluble protein by centrifugation. Protein concentration of the crude enzyme solution was quantified with Protein Assay (Bio-Rad) by using bovine serum albumin as a standard sample.
EXAMPLE 3
Evaluation of GDH Gene-Amplified Strain
[0168] (1) Construction of gdh-amplified strain and evaluation of culture
[0169] Construction of a plasmid pGDH into which the gdh gene of coryneform bacteria was cloned was performed as follows. First, chromosome DNA of Brevibacterium lactofermentum ATCC 13869 strain was extracted, and PCR was performed by using the chromosome DNA as a template and the synthetic DNAs shown in Sequence Listing as SEQ ID NOS: 12 and 13 as primers. The obtained DNA fragment was blunt-ended and inserted into the SmaI site of pHSG399 (Takara Shuzo). This plasmid was designated as pHSG399GDH.
[0170] Then, a replication origin derived from the plasmid pHM1519 ( Agric. Biol. Chem., 48, 2901-2903 (1984)) that could autonomously replicate in coryneform bacteria was introduced into the SalI site of pHSG399GDH. Specifically, the aforementioned pHK4 was digested with restriction enzymes BamHI and KpnI to obtain a gene fragment containing the replication origin, and the obtained fragment was blunt-ended and inserted into the SalI site of pHSG399GDH by using an SalI linker (Takara Shuzo). This plasmid was designated as pGDH.
[0171] The L-glutamine producing bacterium, Brevibacterium flavum AJ12418 strain, was transformed with pGDH to obtain a transformant. Culture for L-glutamine production was performed by the method described in Example 1 using the obtained transformant AJ12418/pGDH. The results are shown in Table 3. In the GDH-enhanced strain, yield of L-glutamine decreased and by-production of L-glutamic acid increased, but culture time was considerably shortened.
TABLE 3 Culture time Strain L-Gln (g/L) L-Glu (g/L) (hr) AJ12418 38.4 0.7 70 AJ12418/pGDH 29.5 12.0 55

EXAMPLE 4
Construction and Evaluation of Strain in which GS and GDH are Enhanced Simultaneously
[0172] (1) Construction of gdh promoter-modified plasmid
[0173] Chromosomal DNA of Brevibacterium flavum ATCC 14067 strain was extracted, and PCR was performed by using the chromosomal DNA as a template and the synthetic DNAs shown in Sequence Listing as SEQ ID NOS: 14 and 15 as primers. The obtained DNA fragment was digested with restriction enzymes StuI and PvuII and inserted into the SmaI site of pHSG399. This plasmid was digested with a restriction enzyme SacI to obtain a DNA fragment containing the gdh promoter and a partial fragment of the gdh gene, and it was inserted into the SacI site of pKF19k (Takara Shuzo). This plasmid was designated as pKF19GDH.
[0174] A mutation was introduced into the promoter region by using Mutan-Super Express Km (Takara Shuzo). LA-PCR was performed by using pkF19GDH as a template, a selection primer attached to Mutan-super Express Km and a 5′-end phosphorylated synthetic DNA shown in Sequence Listing as SEQ ID NO: 16 or 17 as a primer for mutagenesis. The reaction product was purified by ethanol precipitation, and competent cells of Escherichia coli MV1184 (Takara Shuzo) were transformed with the product to obtain transformants.
[0175] Plasmids were extracted from the transformants, and sequences of the gdh promoter region were determined. Among these, those having the sequences shown in Table 4 were designated as pKF19GDH1 and pKF19GDH4. It is expected that the GDH activity can be improved by about 3 times by replacing the gdh promoter sequence with that of pKF19GDH1 type, or by about 5 times by replacing the gdh promoter sequence with that of pKF19GDH4 type, compared with gdh having a promoter of a wild-type (refer to International Patent Publication WO00/18935).
[0176] These plasmids were digested with a restriction enzyme SacI to obtain a DNA fragment containing the gdh promoter and a partial fragment of the gdh gene, and it was inserted into the SacI site of pSFKT2 (refer to Japanese Patent Laid-open Publication No. 2000-262288). These plasmids were designated as pSFKTGDH1 and pSFKTGDH4, respectively. pSFKT2 was a derivative of the plasmid pAM330 derived from the Brevibacterium lactofermentum ATCC 13869 strain, and it is a plasmid of which autonomous replication in coryneform bacteria has become temperature sensitive.
TABLE 4 Plasmid gdh promoter sequence pKF19GDH TGGTCAtatctgtgcgacgctgcCATAAT (SEQ ID NO: 20) pKF19GDH1 TGGTCAtatctgtgcgacgctgcTATAAT (SEQ ID NO: 21) pKF19GDH4 TTGCCAtatctgtgcgacgctgcTATAAT (SEQ ID NO: 22)

[0177] (2) Introduction of gdh promoter mutation into chromosome
[0178] A mutation was introduced into the gdh promoter sequence on chromosome as follows. First, the QA-1 strain was transformed with the plasmid pSFKTGDH1 or pSFKTGDH4 by the electric pulse method to obtain a transformant, respectively. After the transformation, culture was performed at 25° C. Then, these transformants were cultured at 34° C., and strains showing kanamycin resistance at 34° C. were selected. Since the aforementioned plasmids cannot autonomously replicate at 34° C., only those in which these plasmids were integrated into chromosome by homologous recombination show kanamycin resistance. Further, the strains in which these plasmids were integrated into chromosome were cultured in the absence of kanamycin, and strains that became kanamycin sensitive were selected. Among those, strains in which the same mutation as that of pSFKTGDH1 or pSFKTGDH4 was introduced into the gdh promoter region on the chromosome were designated as QB-1 and QB-4, respectively.
[0179] (3) Construction of gdh gene-amplified strain and measurement of GDH activity
[0180] The L-glutamine producing bacterium, Brevibacterium flavum QA-1 strain, was transformed with the plasmid pGDH described in Example 3, (2) to obtain a transformant. Culture for L-glutamine production was performed by the method described in Example 1 using the obtained transformant QA-1/pGDH. The GDH activity was measured by adding a crude enzyme solution to a solution containing 100 mM Tris-HCl (pH 7.5), 20 mM NH 4 Cl, 10 mM α-ketoglutaric acid and 0.25 mM NADPH and measuring change of absorbance at 340 nm referring to Mol. Microbiology, 317-326 (6) 1992. The crude enzyme solution was prepared by separating cells from the aforementioned culture broth by centrifugation, washing the cells with 100 mM Tris-HCl (pH 7.5), sonicating the cells and removing undisrupted cells by centrifugation. Protein concentration of the crude enzyme solution was quantified with Protein Assay (Bio-Rad) by using bovine serum albumin as a standard sample. The results are shown in Table 5.
[0181] As for yield of L-glutamine, the GDH promoter-modified strains, QB-1 and QB-4, showed high yield. Further, the QA-1/pGDH strain also showed higher yield than that obtained with the AJ12418 strain. The culture time of the QA-1/pGDH strain was the shortest. The by-production of L-glutamic acid was markedly improved in the QB-1 and QB-4 strains. From these results, it was demonstrated that the simultaneous enhancement of GS and GDH was effective for improvement of yield of L-glutamine and shortening of culture time.
TABLE 5 L-Gln L-Glu Culture GDH activity Strain (g/L) (g/L) time (hr) (U/mg) AJ12418 40.5 0.8 68 1.6 QA-1/PGDH 47.9 1.0 60 15.2 QB-1 50.5 0.1 65 4.2. QB-4 50.0 0.3 65 9.6

EXAMPLE 5
Acquisition of Gene Coding for Isozyme of GS
[0182] In the paper that reported acquisition of glnA of Corynebacterium glutamicum ( FEMS Microbiol. Letter, 154 (1997) 81-88), it is described that a ΔglnA-disrupted strain became to show glutamine auxotrophy and lost the GS activity, and it also reported data showing results of Southern blotting and suggesting existence of an isozyme. Further, “Amino Acid Fermentation”, Japan Science Societies Publication (Gakkai Shuppan Center), pp.232-235 describes that there are two kinds of GS for Corynebacterium glutamicum . Therefore, it was attempted to obtain a gene coding for the second GS isozyme.
[0183] (1) Preparation of probe
[0184] A gene coding for an isozyme of GS (glnA2) was obtained by colony hybridization. First, PCR was performed by using the primers shown in Sequence Listing as SEQ ID NOS: 18 and 19 and chromosomal DNA of the Brevibacterium lactofermentum ATCC 13869 strain as a template to obtain a partial fragment of the glnA gene. This DNA fragment was labeled by using DIG-High Prime DNA Labeling & Detection Starter Kit I (Boehringer Mannheim) and used as a probe.
[0185] (2) Colony hybridization
[0186] Chromosomal DNA of the Brevibacterium lactofermentum ATCC 13869 strain was extracted and partially digested with a restriction enzyme Sau3AI, and the obtained DNA fragment was inserted into the BamHI site of the vector of pHSG299 and used to transform the Escherichia coli JM109 strain. The obtained transformant was transferred to Hybond-N+ (Amersham Pharmacia Biotech), denatured, neutralized and then hybridized with the probe prepared in Example 5, (1) by using DIG-High Prime DNA Labeling & Detection Starter Kit I. At this time, a transformant that hybridized strongly and a transformant that hybridized weakly were recognized. Plasmid DNAs were prepared from these transformants and nucleotide sequences of inserts were determined. As a result, clones containing a gene showing high homology with respect to a known glutamine synthetase of coryneform bacteria could be obtained. The total nucleotide sequence of the insert of the latter was shown in Sequence Listing as SEQ ID NO: 1.
[0187] Open reading frames were deduced, and amino acid sequences deduced from the nucleotide sequences were shown in Sequence Listing as SEQ ID NOS: 2 and 3. Each of these amino acid sequences was compared with known sequences for homology. The used database was Genbank. As a result, it became clear that the amino acid sequences encoded by the both of the open reading frames were novel proteins of coryneform bacteria.
[0188] The nucleotide sequences and the amino acid sequences were analyzed by using Genetyx-Mac computer program (Software Development, Tokyo). The homology analysis was performed according to the method of Lipman and Pearson ( Science, 227, 1435-1441, 1985).
[0189] The amino acid sequence shown in Sequence Listing as SEQ ID NO: 2 showed 34.6%, 65.6% and 60% of homology with respect to already reported GS of Corynebacterium glutamicum ( FEMS Microbiology Letters, 81-88, (154) 1997), GS of Mycobacterium tuberculosis (GenBank accession Z70692) and GS of Streptomyces coelicolor (GenBank accession AL136500), respectively (Table 6), and it was found to be an isozyme of GS of coryneform bacteria.
[0190] On the other hand, the sequence shown in Sequence Listing as SEQ ID NO: 3 showed 51.9% and 33.4% of homology with respect to the already reported ATase of Mycobacterium tuberculosis (GenBank accession Z70692) and ATase of Streptomyces coelicolor (GenBank accession Y17736), respectively (Table 7), and it was found to be ATase of coryneform bacteria. Therefore, it was found that, in the nucleotide sequence shown as SEQ ID NO: 1, the open reading frame coding for the amino acid sequence shown as SEQ ID NO: 2 was glnA2, and the open reading frame coding for the amino acid sequence shown as SEQ ID NO: 3 was glnE.
TABLE 6 Gene Amino acid Strain name Number Homology Brevibacterium glnA2 446 A.A. — lactofermentum Corynebacterium glutamicum glnA 478 A.A. 34.6% Mycobacterium tuberculosis glnA2 446 A.A. 65.6% Streptomyces coelicolor glnA 453 A.A. 60.0%

[0191] TABLE 7 Gene Amino acid Strain name Number Homology Brevibacterium glnE 1045 A.A. — lactofermentum Mycobacterium tuberculosis glnE 994 A.A. 51.9% Streptomyces coelicolor glnE 784 A.A. 33.4%
EXAMPLE 6
Production of L-glutamine by ATase-Deficient Strain
[0192] Since the gene glnE coding for ATase was elucidated in the aforementioned Example 5, a glnE-deficient strain was constructed from the L-glutamine producing bacterium AJ12418. The specific procedure will be shown below.
[0193] First, PCR was performed by using chromosome DNA of Brevibacterium flavum ATCC 14067 strain as a template and the synthetic DNAs of SEQ ID NOS: 23 and 24 as primers to obtain a partial fragment of glnE gene. The produced PCR product was purified in a conventional manner, then blunt-ended and inserted into the HincII site of pHSG299 (Takara Shuzo). This plasmid was designated as pGLNE. Then, in order to delete a partial region of the glnE gene in this plasmid, pGLNE was digested with HincII and self-ligated, and the obtained plasmid was designated as pΔGLNE. This plasmid contained the 2341st to 4650th nucleotides of the nucleotide sequence shown in Sequence Listing as SEQ ID NO: 1, but it had deletion of about 300 bp from the 3343rd HincII recognition site to the 3659th HincII recognition site.
[0194] Since the above pΔGLNE does not contain a region that enables its autonomous replication within cells of coryneform bacteria, when a coryneform bacterium is transformed with this plasmid, a strain in which the plasmid is integrated into chromosome by homologous recombination may be produced as a transformant although it occurs at an extremely low frequency.
[0195] The L-glutamine producing bacterium, Brevibacterium flavum AJ12418, was transformed with the plasmid pΔGLNE at a high concentration by the electric pulse method, and transformants were obtained by using kanamycin resistance as a marker. Then, these transformants were subcultured to obtain strains that became kanamycin sensitive. Further, chromosomal DNAs of the obtained kanamycin sensitive strains were extracted, and PCR was performed by using each chromosomal DNA as a template and the synthetic DNAs shown in Sequence Listing as SEQ ID NOS: 23 and 24 as primers to obtain partial fragments of the glnE gene. A strain of which PCR product did not provide about 300 bp fragment when it was digested with HincII was determined as a glnE-disrupted strain. This strain was designated as QA-T. Culture for L-glutamine production was performed in the same manner as described in Example 1,
[0196] (3) by using AJ12418 and QA-T strains. The results are shown in Table 8.
[0197] The QA-T strain showed improvement of L-glutamine accumulation compared with the AJ12418 strain. The results of measurement of the GS activity of these strains are also shown in Table 8. It was confirmed that the GS activity was improved in the QA-T strain compared with the AJ12418 strain.
TABLE 8 GS activity Culture time Strain L-Gln (g/L) (U/mg) (hr) AJ12418 39.0 0.03 70 QA-T 45.1 0.05 75

[0198] (EXPLANATION OF SEQUENCE LISTING)
[0199] SEQ ID NO: 1: glnA2 And glnE nucleotide sequences
[0200] SEQ ID NO: 2: glnA2 amino acid sequence
[0201] SEQ ID NO: 3: glnE amino acid sequence
[0202] SEQ ID NO: 4: Primer N for glnA amplification
[0203] SEQ ID NO: 5: Primer C for glnA amplification
[0204] SEQ ID NO: 6: glnA 1st PCR primer NN
[0205] SEQ ID NO: 7: glnA 1st PCR primer NC
[0206] SEQ ID NO: 8: glnA 1st PCR primer CN
[0207] SEQ ID NO: 9: glnA 1st PCR primer CC
[0208] SEQ ID NO: 10: glnA 2nd PCR primer N
[0209] SEQ ID NO: 11: glnA 2nd PCR primer C
[0210] SEQ ID NO: 12: Primer N for gdh amplification
[0211] SEQ ID NO: 13: Primer C for gdh amplification
[0212] SEQ ID NO: 14: Primer N2 for gdb amplification
[0213] SEQ ID NO: 15: Primer C2 for gdb amplification
[0214] SEQ ID NO: 16: Primer M1 for gdh promoter mutation
[0215] SEQ ID NO: 17: Primer M4 for gdh promoter mutation
[0216] SEQ ID NO: 18: Primer N for glnA probe preparation
[0217] SEQ ID NO: 19: Primer C for glnA probe preparation
[0218] SEQ ID NO: 20: Wild-type gdh promoter sequence
[0219] SEQ ID NO: 21: Mutant type gdh promoter sequence
[0220] SEQ ID NO: 22: Mutant type gdh promoter sequence
[0221] SEQ ID NO: 23: Primer N for glnE disruption
[0222] SEQ ID NO: 24: Primer C for glnE disruption



1


25


1
5500
DNA
Brevibacterium lactofermentum

CDS
(659)..(1996)



1

gatcagtgct tcggcttctt cttcgaagtt ggtggactct gcctttttca aaagtgcggt 60

gatacgatga tgcgctttgg cctgtgccgg ggtcattggg ctggtgtctt gattgtctaa 120

ggcgtggagc tctgcgagca ttgcccagtc aggcaaggta cttagcttcg gtagctcggt 180

gagaatcttc tccagggtca tcaccggcaa gtggctagtt tcggcggcac gcgttccgtt 240

cacccacagt gtgtacatct catcggagca ggagtaagca atctcaggta gcgcgtgaaa 300

caggagtgga tcaatatcgg cggaaaactc atggcggaga tcggcgggag tccacccacg 360

aagcgcacag aaacctaggt ggctgatgat gctttcttct aaaatctgac ggtaagagtc 420

ttgtgcgtcg gtgacgttgt cggagaagtg ggagagggtc attgcggttt ccttattcgt 480

aggagagttc taatttcggt gcggttctca gtgaaccacc caagctggac acctcccacc 540

cccgtgtcat caaaaaaccg cgacatcctt gagtaactct gagaaaaact acccccgatg 600

cgagtataaa agtggcaaat gcgcagtcga tgtcccatcg ctgcgtagat tagttttc 658

atg aac agc gaa cag gaa ttt gta ctc agc gcc att gaa gaa cgc gac 706
Met Asn Ser Glu Gln Glu Phe Val Leu Ser Ala Ile Glu Glu Arg Asp
1 5 10 15

att aag ttt gtg cgt cta tgg ttc act gac att ctt gga cac ttg aag 754
Ile Lys Phe Val Arg Leu Trp Phe Thr Asp Ile Leu Gly His Leu Lys
20 25 30

tca gtg gtt gtg gct cct gca gaa cta gag tct gcg ttg gaa gaa ggc 802
Ser Val Val Val Ala Pro Ala Glu Leu Glu Ser Ala Leu Glu Glu Gly
35 40 45

atc gga ttc gat ggc tca gcc att gag ggc tac gcg cgt atc tcg gaa 850
Ile Gly Phe Asp Gly Ser Ala Ile Glu Gly Tyr Ala Arg Ile Ser Glu
50 55 60

gcg gac acc att gcc cgc cca gat cca tcg aca ttc cag gtc ctc cca 898
Ala Asp Thr Ile Ala Arg Pro Asp Pro Ser Thr Phe Gln Val Leu Pro
65 70 75 80

cta gaa gcg ggc atc tca aaa ctg cag gca gca cgc ctg ttt tgc gat 946
Leu Glu Ala Gly Ile Ser Lys Leu Gln Ala Ala Arg Leu Phe Cys Asp
85 90 95

gtc acg atg ccg gac gga cag cca tct ttt tct gac ccg cgc caa gtg 994
Val Thr Met Pro Asp Gly Gln Pro Ser Phe Ser Asp Pro Arg Gln Val
100 105 110

ctg cgc agg cag gtc caa cta gct gca gat gaa ggc ttg acc tgc atg 1042
Leu Arg Arg Gln Val Gln Leu Ala Ala Asp Glu Gly Leu Thr Cys Met
115 120 125

atc tca cca gag att gag ttc tat ttg gtg caa agc ctt cgc acc aac 1090
Ile Ser Pro Glu Ile Glu Phe Tyr Leu Val Gln Ser Leu Arg Thr Asn
130 135 140

gga ctg cca cct gtg ccc act gac aac ggc gga tat ttc gac caa gcc 1138
Gly Leu Pro Pro Val Pro Thr Asp Asn Gly Gly Tyr Phe Asp Gln Ala
145 150 155 160

aca ttc aat gag gcg ccg aat ttc cgt cga aac gcg atg gta gcg ctg 1186
Thr Phe Asn Glu Ala Pro Asn Phe Arg Arg Asn Ala Met Val Ala Leu
165 170 175

gag gaa ctc ggc atc cct gtc gag ttc tcc cac cat gaa act gca cct 1234
Glu Glu Leu Gly Ile Pro Val Glu Phe Ser His His Glu Thr Ala Pro
180 185 190

ggc cag caa gaa atc gat tta cgc cat gcg gat gcg ctc acc atg gcc 1282
Gly Gln Gln Glu Ile Asp Leu Arg His Ala Asp Ala Leu Thr Met Ala
195 200 205

gac aac atc atg acc ttc cgc tac atc atg aaa cag gtg gca agg gac 1330
Asp Asn Ile Met Thr Phe Arg Tyr Ile Met Lys Gln Val Ala Arg Asp
210 215 220

caa ggc gtt ggg gca tca ttt atg ccc aag cca ttc caa gaa cat gca 1378
Gln Gly Val Gly Ala Ser Phe Met Pro Lys Pro Phe Gln Glu His Ala
225 230 235 240

ggc tcc gcc atg cac acg cac atg tcc tta ttt gag ggc gat acc aac 1426
Gly Ser Ala Met His Thr His Met Ser Leu Phe Glu Gly Asp Thr Asn
245 250 255

gcg ttc cac gat cca gac gat tct tac atg ctg tcc aaa acc gca aaa 1474
Ala Phe His Asp Pro Asp Asp Ser Tyr Met Leu Ser Lys Thr Ala Lys
260 265 270

cag ttc atc gct gga atc ttg cat cac gct cca gaa ttc acc gct gtg 1522
Gln Phe Ile Ala Gly Ile Leu His His Ala Pro Glu Phe Thr Ala Val
275 280 285

acc aac cag tgg gtc aat tcc tac aaa cgc atc gtg tac gga aac gaa 1570
Thr Asn Gln Trp Val Asn Ser Tyr Lys Arg Ile Val Tyr Gly Asn Glu
290 295 300

gct cca act gcg gca acc tgg ggt gta tct aat cgt tct gcg ctg gtt 1618
Ala Pro Thr Ala Ala Thr Trp Gly Val Ser Asn Arg Ser Ala Leu Val
305 310 315 320

cgt gtt cct acc tac cgt ttg aat aag gag gag tcg cgc cgg gtg gag 1666
Arg Val Pro Thr Tyr Arg Leu Asn Lys Glu Glu Ser Arg Arg Val Glu
325 330 335

gtg cgt ctt cct gat acc gct tgt aac cca tat ttg gcg ttt tca gtg 1714
Val Arg Leu Pro Asp Thr Ala Cys Asn Pro Tyr Leu Ala Phe Ser Val
340 345 350

atg ctc ggc gct ggt ttg aaa ggt att aaa gaa ggt tat gag ctc gac 1762
Met Leu Gly Ala Gly Leu Lys Gly Ile Lys Glu Gly Tyr Glu Leu Asp
355 360 365

gag cca gct gag gac gat atc tcc aac ttg agc ttc cgg gaa cgt cgc 1810
Glu Pro Ala Glu Asp Asp Ile Ser Asn Leu Ser Phe Arg Glu Arg Arg
370 375 380

gcc atg ggc tac aac gat ctg cca aac agc ctt gat cag gca ctg cgc 1858
Ala Met Gly Tyr Asn Asp Leu Pro Asn Ser Leu Asp Gln Ala Leu Arg
385 390 395 400

caa atg gaa aag tca gag ctt gtt gct gac atc ctc ggt gag cac gtt 1906
Gln Met Glu Lys Ser Glu Leu Val Ala Asp Ile Leu Gly Glu His Val
405 410 415

ttt gag ttt ttc ttg cgc aat aag tgg cgt gaa tgg cgt gac tac caa 1954
Phe Glu Phe Phe Leu Arg Asn Lys Trp Arg Glu Trp Arg Asp Tyr Gln
420 425 430

gag cag atc act ccg tgg gag ctc cga aac aat ctt gat tac tagactttt 2005
Glu Gln Ile Thr Pro Trp Glu Leu Arg Asn Asn Leu Asp Tyr
435 440 445

gca ctc caa tgg aaa ccc tac ggc gac cca att gcg acc cga taa agg 2053
Ala Leu Gln Trp Lys Pro Tyr Gly Asp Pro Ile Ala Thr Arg Arg
450 455 460

agg gga gaa gct atg tca gga ccg tta aga agt gaa cgt aaa gtc gtt 2101
Arg Gly Glu Ala Met Ser Gly Pro Leu Arg Ser Glu Arg Lys Val Val
465 470 475

ggc ttt gtc aga gac cca ctg cca aaa gtt ggt tct tta tcg ctg aaa 2149
Gly Phe Val Arg Asp Pro Leu Pro Lys Val Gly Ser Leu Ser Leu Lys
480 485 490

tct gag cat gcc caa gca gat cta gag cat ttg ggt tgg cgc aat gtt 2197
Ser Glu His Ala Gln Ala Asp Leu Glu His Leu Gly Trp Arg Asn Val
495 500 505

gag tct ttg gat ttg ttg tgg ggc ttg tca ggt gca ggc gat ccc gat 2245
Glu Ser Leu Asp Leu Leu Trp Gly Leu Ser Gly Ala Gly Asp Pro Asp
510 515 520 525

gtc gcg ctg aac ctt ctt att cgg ctg tat cag gca ctt gaa gca atc 2293
Val Ala Leu Asn Leu Leu Ile Arg Leu Tyr Gln Ala Leu Glu Ala Ile
530 535 540

ggc gag gat gct cga aac gag ctt gat caa gag att cgc cag gat gaa 2341
Gly Glu Asp Ala Arg Asn Glu Leu Asp Gln Glu Ile Arg Gln Asp Glu
545 550 555

gaa cta cga gtc cgc ctt ttt gca ttg ttg ggt ggt tcc tcg gct gtc 2389
Glu Leu Arg Val Arg Leu Phe Ala Leu Leu Gly Gly Ser Ser Ala Val
560 565 570

ggt gat cac ttg gtc gcc aat cct ttg cag tgg aaa ctc tta aaa ctt 2437
Gly Asp His Leu Val Ala Asn Pro Leu Gln Trp Lys Leu Leu Lys Leu
575 580 585

gat gcg cca tcg agg gaa gag atg ttt cag gcg ctg ctg gaa tct gtg 2485
Asp Ala Pro Ser Arg Glu Glu Met Phe Gln Ala Leu Leu Glu Ser Val
590 595 600 605

aaa gct cag cct gct gtg ctt gag gtt gag gat ttc agc gat gca cac 2533
Lys Ala Gln Pro Ala Val Leu Glu Val Glu Asp Phe Ser Asp Ala His
610 615 620

aac att gcc cga gac gat ttg agc acg cct ggt ttt tac acg gct agt 2581
Asn Ile Ala Arg Asp Asp Leu Ser Thr Pro Gly Phe Tyr Thr Ala Ser
625 630 635

gtt acc ggg cct gaa gca gag cga gtc ttg aaa tgg act tat cgc acg 2629
Val Thr Gly Pro Glu Ala Glu Arg Val Leu Lys Trp Thr Tyr Arg Thr
640 645 650

ttg ctg acc cgg att gct gcg cat gat tta gcg ggt acc tat ccc acc 2677
Leu Leu Thr Arg Ile Ala Ala His Asp Leu Ala Gly Thr Tyr Pro Thr
655 660 665

gac atg cgg aga aaa ggt ggc gat cct gtt ccg ttt agc aca gtg acc 2725
Asp Met Arg Arg Lys Gly Gly Asp Pro Val Pro Phe Ser Thr Val Thr
670 675 680 685

atg cag ctc agc gac cta gct gat gct gct ttg act gct gct tta gct 2773
Met Gln Leu Ser Asp Leu Ala Asp Ala Ala Leu Thr Ala Ala Leu Ala
690 695 700

gtg gca att gcc aat gtt tat ggt gaa aag ccg gtt gat tca gct tta 2821
Val Ala Ile Ala Asn Val Tyr Gly Glu Lys Pro Val Asp Ser Ala Leu
705 710 715

tct gtc atc gcg atg ggc aaa tgt ggc gcg cag gaa ttg aac tac att 2869
Ser Val Ile Ala Met Gly Lys Cys Gly Ala Gln Glu Leu Asn Tyr Ile
720 725 730

tca gat gtg gac gtg gtg ttt gtt gca gag ccg gca aac tct aaa tca 2917
Ser Asp Val Asp Val Val Phe Val Ala Glu Pro Ala Asn Ser Lys Ser
735 740 745

aca cgc acc gca gca gag ctc att cgc atc ggt agc aac tcg ttc ttt 2965
Thr Arg Thr Ala Ala Glu Leu Ile Arg Ile Gly Ser Asn Ser Phe Phe
750 755 760 765

gag gtg gat gca gca ctt cgc cca gaa ggt aaa agt ggc gct ctt gtg 3013
Glu Val Asp Ala Ala Leu Arg Pro Glu Gly Lys Ser Gly Ala Leu Val
770 775 780

cgc tct ttg gat tcc cat atg gcg tat tac aag cgc tgg gcg gaa acc 3061
Arg Ser Leu Asp Ser His Met Ala Tyr Tyr Lys Arg Trp Ala Glu Thr
785 790 795

tgg gaa ttt cag gca ctg ctg aaa gct cgt ccc atg acg ggt gat att 3109
Trp Glu Phe Gln Ala Leu Leu Lys Ala Arg Pro Met Thr Gly Asp Ile
800 805 810

gac ctt ggg cag tcc tat gtg gat gct ctt tca ccg ttg att tgg gcg 3157
Asp Leu Gly Gln Ser Tyr Val Asp Ala Leu Ser Pro Leu Ile Trp Ala
815 820 825

gct agc cag cgg gaa tca ttt gtc aca gat gtc caa gct atg cgc cgt 3205
Ala Ser Gln Arg Glu Ser Phe Val Thr Asp Val Gln Ala Met Arg Arg
830 835 840 845

cga gtg ttg gac aat gtt ccg gaa gac ttg cgt gat cgt gag ctg aag 3253
Arg Val Leu Asp Asn Val Pro Glu Asp Leu Arg Asp Arg Glu Leu Lys
850 855 860

ctt ggt cgc ggt ggt ttg agg gat gtg gag ttt gct gtc cag ctc ctt 3301
Leu Gly Arg Gly Gly Leu Arg Asp Val Glu Phe Ala Val Gln Leu Leu
865 870 875

cag atg gtg cat ggt cgc att gat gag acg ttg cgg gtt cgg tca acg 3349
Gln Met Val His Gly Arg Ile Asp Glu Thr Leu Arg Val Arg Ser Thr
880 885 890

gta aat gct ttg cat gtg ttg gtt gat cag gga tat gtg ggt cgt gaa 3397
Val Asn Ala Leu His Val Leu Val Asp Gln Gly Tyr Val Gly Arg Glu
895 900 905

gac ggg cat aat ctc att gag tcg tat gag ttt ttg cgc ctg ttg gag 3445
Asp Gly His Asn Leu Ile Glu Ser Tyr Glu Phe Leu Arg Leu Leu Glu
910 915 920 925

cat cgc ctt caa ttg gag cgg atc aag cgc act cac ttg tta ccg aaa 3493
His Arg Leu Gln Leu Glu Arg Ile Lys Arg Thr His Leu Leu Pro Lys
930 935 940

cct gat gac cga atg aat atg cgc tgg ttg gcg cgc gct tct ggg ttt 3541
Pro Asp Asp Arg Met Asn Met Arg Trp Leu Ala Arg Ala Ser Gly Phe
945 950 955

act ggt tcg atg gag caa agt tcg gcc aaa gct atg gaa cgg cat ttg 3589
Thr Gly Ser Met Glu Gln Ser Ser Ala Lys Ala Met Glu Arg His Leu
960 965 970

cgt aag gtt cgt ttg cag att cag tcg ttg cat agt cag ctg ttt tat 3637
Arg Lys Val Arg Leu Gln Ile Gln Ser Leu His Ser Gln Leu Phe Tyr
975 980 985

cgg cca ctg ctg aac tct gtg gtc aac ttg agc gcg gat gcc atc aga 3685
Arg Pro Leu Leu Asn Ser Val Val Asn Leu Ser Ala Asp Ala Ile Arg
990 995 1000 1005

ttg tct ccg gat gct gca aag cta caa ttg ggg gca ttg gga tac 3730
Leu Ser Pro Asp Ala Ala Lys Leu Gln Leu Gly Ala Leu Gly Tyr
1010 1015 1020

ctg cat cca tca cgt gct tat gaa cac ctg act gct ctt gca tca 3775
Leu His Pro Ser Arg Ala Tyr Glu His Leu Thr Ala Leu Ala Ser
1025 1030 1035

gga gct agc cgt aaa gcc aag att cag gcg atg ttg ctg ccc acg 3820
Gly Ala Ser Arg Lys Ala Lys Ile Gln Ala Met Leu Leu Pro Thr
1040 1045 1050

ttg atg gag tgg ctg tct caa aca gct gaa cca gat gcg gga ttg 3865
Leu Met Glu Trp Leu Ser Gln Thr Ala Glu Pro Asp Ala Gly Leu
1055 1060 1065

ctg aat tac cgc aag ctt tct gat gct tcc tat gat cgc agc tgg 3910
Leu Asn Tyr Arg Lys Leu Ser Asp Ala Ser Tyr Asp Arg Ser Trp
1070 1075 1080

ttt ttg cgc atg ctg cgt gat gag ggc gta gtg ggg cag cgg ttg 3955
Phe Leu Arg Met Leu Arg Asp Glu Gly Val Val Gly Gln Arg Leu
1085 1090 1095

atg cgt att ttg gga aat tct ccc tat att tct gaa ctg att atc 4000
Met Arg Ile Leu Gly Asn Ser Pro Tyr Ile Ser Glu Leu Ile Ile
1100 1105 1110

tcc act ccg gac ttt gtg aaa cag ctg ggt gat gcg gcg tct ggt 4045
Ser Thr Pro Asp Phe Val Lys Gln Leu Gly Asp Ala Ala Ser Gly
1115 1120 1125

cct aaa ttg ctt gct act gca ccg act cag gtt gtg aaa gca atc 4090
Pro Lys Leu Leu Ala Thr Ala Pro Thr Gln Val Val Lys Ala Ile
1130 1135 1140

aag gcg acg gtg tcg cgt cat gag tca cct gat cgg gcg atc cag 4135
Lys Ala Thr Val Ser Arg His Glu Ser Pro Asp Arg Ala Ile Gln
1145 1150 1155

gct gca cga tcg ctg agg agg cag gag ctg gca cgc att gcc tct 4180
Ala Ala Arg Ser Leu Arg Arg Gln Glu Leu Ala Arg Ile Ala Ser
1160 1165 1170

gct gat ttg ctc aac atg ctc act gtt cag gaa gta tgc caa agc 4225
Ala Asp Leu Leu Asn Met Leu Thr Val Gln Glu Val Cys Gln Ser
1175 1180 1185

ttg tca cta gtc tgg gat gcg gtg ttg gat gct gcc ttg gat gcg 4270
Leu Ser Leu Val Trp Asp Ala Val Leu Asp Ala Ala Leu Asp Ala
1190 1195 1200

gaa atc cgt gct gca ctt aac gat cca cag aaa cca gat cag cct 4315
Glu Ile Arg Ala Ala Leu Asn Asp Pro Gln Lys Pro Asp Gln Pro
1205 1210 1215

ctg gcc aat att tct gtg atc ggc atg ggc cgt ttg ggt gga gca 4360
Leu Ala Asn Ile Ser Val Ile Gly Met Gly Arg Leu Gly Gly Ala
1220 1225 1230

gaa ctt gga tac ggt tct gat gcc gat gtg atg ttt gta tgc gag 4405
Glu Leu Gly Tyr Gly Ser Asp Ala Asp Val Met Phe Val Cys Glu
1235 1240 1245

ccg gta gcc ggt gtg gaa gag cat gag gcc gtc aca tgg tct att 4450
Pro Val Ala Gly Val Glu Glu His Glu Ala Val Thr Trp Ser Ile
1250 1255 1260

gcg atc tgt gat tcc atg cgg tcg agg ctt gcg cag cct tcc ggt 4495
Ala Ile Cys Asp Ser Met Arg Ser Arg Leu Ala Gln Pro Ser Gly
1265 1270 1275

gat cca cct ttg gag gtg gat ctg ggg ctg cgt cct gaa ggg aga 4540
Asp Pro Pro Leu Glu Val Asp Leu Gly Leu Arg Pro Glu Gly Arg
1280 1285 1290

tct ggt gcg att gtg cgc acc gtt gat tcc tat gtg aag tac tac 4585
Ser Gly Ala Ile Val Arg Thr Val Asp Ser Tyr Val Lys Tyr Tyr
1295 1300 1305

gaa aag tgg ggt gaa act tgg gag att cag gcg ctg ctg agg gct 4630
Glu Lys Trp Gly Glu Thr Trp Glu Ile Gln Ala Leu Leu Arg Ala
1310 1315 1320

gcg tgg gtt gct ggt gat cgt gag ctg ggc att aag ttc ttg gag 4675
Ala Trp Val Ala Gly Asp Arg Glu Leu Gly Ile Lys Phe Leu Glu
1325 1330 1335

tcg att gat cgt ttc cgc tac cca gtt gac ggg gca acg cag gcg 4720
Ser Ile Asp Arg Phe Arg Tyr Pro Val Asp Gly Ala Thr Gln Ala
1340 1345 1350

cag ctt cgt gaa gtt cgt cga att aag gcg agg gtg gat aat gag 4765
Gln Leu Arg Glu Val Arg Arg Ile Lys Ala Arg Val Asp Asn Glu
1355 1360 1365

agg ctt ccg cgc ggg gct gat cga aat acc cat acc aag ctg ggt 4810
Arg Leu Pro Arg Gly Ala Asp Arg Asn Thr His Thr Lys Leu Gly
1370 1375 1380

cgg gga gcg tta act gac atc gag tgg act gtg cag ttg ttg acc 4855
Arg Gly Ala Leu Thr Asp Ile Glu Trp Thr Val Gln Leu Leu Thr
1385 1390 1395

atg atg cat gct cat gag att ccg gag ctg cac aat acg tcg acg 4900
Met Met His Ala His Glu Ile Pro Glu Leu His Asn Thr Ser Thr
1400 1405 1410

ttg gaa gtt ctt gaa gtg ctg gaa aag cat cag att att aac cct 4945
Leu Glu Val Leu Glu Val Leu Glu Lys His Gln Ile Ile Asn Pro
1415 1420 1425

gtg cag gtg cag acg ctt cgg gaa gcg tgg ctg acg gca acg gct 4990
Val Gln Val Gln Thr Leu Arg Glu Ala Trp Leu Thr Ala Thr Ala
1430 1435 1440

gct agg aat gcg ctt gtg ctg gtc agg ggt aag aga tta gat cag 5035
Ala Arg Asn Ala Leu Val Leu Val Arg Gly Lys Arg Leu Asp Gln
1445 1450 1455

tta cct act cct ggt ccg cac ctt gcg cag gtg gct ggt gcg tct 5080
Leu Pro Thr Pro Gly Pro His Leu Ala Gln Val Ala Gly Ala Ser
1460 1465 1470

ggt tgg gat cca aat gag tac cag gag tat ttg gaa aac tat ctg 5125
Gly Trp Asp Pro Asn Glu Tyr Gln Glu Tyr Leu Glu Asn Tyr Leu
1475 1480 1485

aaa gtg acc agg aag agt cgt cag gtt gtt gat gaa gtc ttc tgg 5170
Lys Val Thr Arg Lys Ser Arg Gln Val Val Asp Glu Val Phe Trp
1490 1495 1500

ggt gtg gac tct atg gag caa cgt gag ttt taggtaggtg gtgggagccc 5220
Gly Val Asp Ser Met Glu Gln Arg Glu Phe
1505 1510

caaagttgcg gaaaattgtt ccaactaagg gactatatgt aggtgtggat aacctaagtt 5280

aatcttttgt gagcgtgagg atttctctga ggaatctaga cgcagattaa cttccgcttg 5340

gcagcgaccg ggataacacc gcggttgcgg ccacgcaggc tcacaaagga caccactatg 5400

acaagcatta ttgcaagcaa cagcgaccta tcggaggagc tgcgcaccca cactgcgcgg 5460

gcacatgaag aggccgagca ctcaacgttt atgaatgatc 5500



2
446
PRT
Brevibacterium lactofermentum

2

Met Asn Ser Glu Gln Glu Phe Val Leu Ser Ala Ile Glu Glu Arg Asp
1 5 10 15

Ile Lys Phe Val Arg Leu Trp Phe Thr Asp Ile Leu Gly His Leu Lys
20 25 30

Ser Val Val Val Ala Pro Ala Glu Leu Glu Ser Ala Leu Glu Glu Gly
35 40 45

Ile Gly Phe Asp Gly Ser Ala Ile Glu Gly Tyr Ala Arg Ile Ser Glu
50 55 60

Ala Asp Thr Ile Ala Arg Pro Asp Pro Ser Thr Phe Gln Val Leu Pro
65 70 75 80

Leu Glu Ala Gly Ile Ser Lys Leu Gln Ala Ala Arg Leu Phe Cys Asp
85 90 95

Val Thr Met Pro Asp Gly Gln Pro Ser Phe Ser Asp Pro Arg Gln Val
100 105 110

Leu Arg Arg Gln Val Gln Leu Ala Ala Asp Glu Gly Leu Thr Cys Met
115 120 125

Ile Ser Pro Glu Ile Glu Phe Tyr Leu Val Gln Ser Leu Arg Thr Asn
130 135 140

Gly Leu Pro Pro Val Pro Thr Asp Asn Gly Gly Tyr Phe Asp Gln Ala
145 150 155 160

Thr Phe Asn Glu Ala Pro Asn Phe Arg Arg Asn Ala Met Val Ala Leu
165 170 175

Glu Glu Leu Gly Ile Pro Val Glu Phe Ser His His Glu Thr Ala Pro
180 185 190

Gly Gln Gln Glu Ile Asp Leu Arg His Ala Asp Ala Leu Thr Met Ala
195 200 205

Asp Asn Ile Met Thr Phe Arg Tyr Ile Met Lys Gln Val Ala Arg Asp
210 215 220

Gln Gly Val Gly Ala Ser Phe Met Pro Lys Pro Phe Gln Glu His Ala
225 230 235 240

Gly Ser Ala Met His Thr His Met Ser Leu Phe Glu Gly Asp Thr Asn
245 250 255

Ala Phe His Asp Pro Asp Asp Ser Tyr Met Leu Ser Lys Thr Ala Lys
260 265 270

Gln Phe Ile Ala Gly Ile Leu His His Ala Pro Glu Phe Thr Ala Val
275 280 285

Thr Asn Gln Trp Val Asn Ser Tyr Lys Arg Ile Val Tyr Gly Asn Glu
290 295 300

Ala Pro Thr Ala Ala Thr Trp Gly Val Ser Asn Arg Ser Ala Leu Val
305 310 315 320

Arg Val Pro Thr Tyr Arg Leu Asn Lys Glu Glu Ser Arg Arg Val Glu
325 330 335

Val Arg Leu Pro Asp Thr Ala Cys Asn Pro Tyr Leu Ala Phe Ser Val
340 345 350

Met Leu Gly Ala Gly Leu Lys Gly Ile Lys Glu Gly Tyr Glu Leu Asp
355 360 365

Glu Pro Ala Glu Asp Asp Ile Ser Asn Leu Ser Phe Arg Glu Arg Arg
370 375 380

Ala Met Gly Tyr Asn Asp Leu Pro Asn Ser Leu Asp Gln Ala Leu Arg
385 390 395 400

Gln Met Glu Lys Ser Glu Leu Val Ala Asp Ile Leu Gly Glu His Val
405 410 415

Phe Glu Phe Phe Leu Arg Asn Lys Trp Arg Glu Trp Arg Asp Tyr Gln
420 425 430

Glu Gln Ile Thr Pro Trp Glu Leu Arg Asn Asn Leu Asp Tyr
435 440 445



3
14
PRT
Brevibacterium lactofermentum

3

Ala Leu Gln Trp Lys Pro Tyr Gly Asp Pro Ile Ala Thr Arg
1 5 10



4
1050
PRT
Brevibacterium lactofermentum

4

Arg Arg Gly Glu Ala Met Ser Gly Pro Leu Arg Ser Glu Arg Lys Val
1 5 10 15

Val Gly Phe Val Arg Asp Pro Leu Pro Lys Val Gly Ser Leu Ser Leu
20 25 30

Lys Ser Glu His Ala Gln Ala Asp Leu Glu His Leu Gly Trp Arg Asn
35 40 45

Val Glu Ser Leu Asp Leu Leu Trp Gly Leu Ser Gly Ala Gly Asp Pro
50 55 60

Asp Val Ala Leu Asn Leu Leu Ile Arg Leu Tyr Gln Ala Leu Glu Ala
65 70 75 80

Ile Gly Glu Asp Ala Arg Asn Glu Leu Asp Gln Glu Ile Arg Gln Asp
85 90 95

Glu Glu Leu Arg Val Arg Leu Phe Ala Leu Leu Gly Gly Ser Ser Ala
100 105 110

Val Gly Asp His Leu Val Ala Asn Pro Leu Gln Trp Lys Leu Leu Lys
115 120 125

Leu Asp Ala Pro Ser Arg Glu Glu Met Phe Gln Ala Leu Leu Glu Ser
130 135 140

Val Lys Ala Gln Pro Ala Val Leu Glu Val Glu Asp Phe Ser Asp Ala
145 150 155 160

His Asn Ile Ala Arg Asp Asp Leu Ser Thr Pro Gly Phe Tyr Thr Ala
165 170 175

Ser Val Thr Gly Pro Glu Ala Glu Arg Val Leu Lys Trp Thr Tyr Arg
180 185 190

Thr Leu Leu Thr Arg Ile Ala Ala His Asp Leu Ala Gly Thr Tyr Pro
195 200 205

Thr Asp Met Arg Arg Lys Gly Gly Asp Pro Val Pro Phe Ser Thr Val
210 215 220

Thr Met Gln Leu Ser Asp Leu Ala Asp Ala Ala Leu Thr Ala Ala Leu
225 230 235 240

Ala Val Ala Ile Ala Asn Val Tyr Gly Glu Lys Pro Val Asp Ser Ala
245 250 255

Leu Ser Val Ile Ala Met Gly Lys Cys Gly Ala Gln Glu Leu Asn Tyr
260 265 270

Ile Ser Asp Val Asp Val Val Phe Val Ala Glu Pro Ala Asn Ser Lys
275 280 285

Ser Thr Arg Thr Ala Ala Glu Leu Ile Arg Ile Gly Ser Asn Ser Phe
290 295 300

Phe Glu Val Asp Ala Ala Leu Arg Pro Glu Gly Lys Ser Gly Ala Leu
305 310 315 320

Val Arg Ser Leu Asp Ser His Met Ala Tyr Tyr Lys Arg Trp Ala Glu
325 330 335

Thr Trp Glu Phe Gln Ala Leu Leu Lys Ala Arg Pro Met Thr Gly Asp
340 345 350

Ile Asp Leu Gly Gln Ser Tyr Val Asp Ala Leu Ser Pro Leu Ile Trp
355 360 365

Ala Ala Ser Gln Arg Glu Ser Phe Val Thr Asp Val Gln Ala Met Arg
370 375 380

Arg Arg Val Leu Asp Asn Val Pro Glu Asp Leu Arg Asp Arg Glu Leu
385 390 395 400

Lys Leu Gly Arg Gly Gly Leu Arg Asp Val Glu Phe Ala Val Gln Leu
405 410 415

Leu Gln Met Val His Gly Arg Ile Asp Glu Thr Leu Arg Val Arg Ser
420 425 430

Thr Val Asn Ala Leu His Val Leu Val Asp Gln Gly Tyr Val Gly Arg
435 440 445

Glu Asp Gly His Asn Leu Ile Glu Ser Tyr Glu Phe Leu Arg Leu Leu
450 455 460

Glu His Arg Leu Gln Leu Glu Arg Ile Lys Arg Thr His Leu Leu Pro
465 470 475 480

Lys Pro Asp Asp Arg Met Asn Met Arg Trp Leu Ala Arg Ala Ser Gly
485 490 495

Phe Thr Gly Ser Met Glu Gln Ser Ser Ala Lys Ala Met Glu Arg His
500 505 510

Leu Arg Lys Val Arg Leu Gln Ile Gln Ser Leu His Ser Gln Leu Phe
515 520 525

Tyr Arg Pro Leu Leu Asn Ser Val Val Asn Leu Ser Ala Asp Ala Ile
530 535 540

Arg Leu Ser Pro Asp Ala Ala Lys Leu Gln Leu Gly Ala Leu Gly Tyr
545 550 555 560

Leu His Pro Ser Arg Ala Tyr Glu His Leu Thr Ala Leu Ala Ser Gly
565 570 575

Ala Ser Arg Lys Ala Lys Ile Gln Ala Met Leu Leu Pro Thr Leu Met
580 585 590

Glu Trp Leu Ser Gln Thr Ala Glu Pro Asp Ala Gly Leu Leu Asn Tyr
595 600 605

Arg Lys Leu Ser Asp Ala Ser Tyr Asp Arg Ser Trp Phe Leu Arg Met
610 615 620

Leu Arg Asp Glu Gly Val Val Gly Gln Arg Leu Met Arg Ile Leu Gly
625 630 635 640

Asn Ser Pro Tyr Ile Ser Glu Leu Ile Ile Ser Thr Pro Asp Phe Val
645 650 655

Lys Gln Leu Gly Asp Ala Ala Ser Gly Pro Lys Leu Leu Ala Thr Ala
660 665 670

Pro Thr Gln Val Val Lys Ala Ile Lys Ala Thr Val Ser Arg His Glu
675 680 685

Ser Pro Asp Arg Ala Ile Gln Ala Ala Arg Ser Leu Arg Arg Gln Glu
690 695 700

Leu Ala Arg Ile Ala Ser Ala Asp Leu Leu Asn Met Leu Thr Val Gln
705 710 715 720

Glu Val Cys Gln Ser Leu Ser Leu Val Trp Asp Ala Val Leu Asp Ala
725 730 735

Ala Leu Asp Ala Glu Ile Arg Ala Ala Leu Asn Asp Pro Gln Lys Pro
740 745 750

Asp Gln Pro Leu Ala Asn Ile Ser Val Ile Gly Met Gly Arg Leu Gly
755 760 765

Gly Ala Glu Leu Gly Tyr Gly Ser Asp Ala Asp Val Met Phe Val Cys
770 775 780

Glu Pro Val Ala Gly Val Glu Glu His Glu Ala Val Thr Trp Ser Ile
785 790 795 800

Ala Ile Cys Asp Ser Met Arg Ser Arg Leu Ala Gln Pro Ser Gly Asp
805 810 815

Pro Pro Leu Glu Val Asp Leu Gly Leu Arg Pro Glu Gly Arg Ser Gly
820 825 830

Ala Ile Val Arg Thr Val Asp Ser Tyr Val Lys Tyr Tyr Glu Lys Trp
835 840 845

Gly Glu Thr Trp Glu Ile Gln Ala Leu Leu Arg Ala Ala Trp Val Ala
850 855 860

Gly Asp Arg Glu Leu Gly Ile Lys Phe Leu Glu Ser Ile Asp Arg Phe
865 870 875 880

Arg Tyr Pro Val Asp Gly Ala Thr Gln Ala Gln Leu Arg Glu Val Arg
885 890 895

Arg Ile Lys Ala Arg Val Asp Asn Glu Arg Leu Pro Arg Gly Ala Asp
900 905 910

Arg Asn Thr His Thr Lys Leu Gly Arg Gly Ala Leu Thr Asp Ile Glu
915 920 925

Trp Thr Val Gln Leu Leu Thr Met Met His Ala His Glu Ile Pro Glu
930 935 940

Leu His Asn Thr Ser Thr Leu Glu Val Leu Glu Val Leu Glu Lys His
945 950 955 960

Gln Ile Ile Asn Pro Val Gln Val Gln Thr Leu Arg Glu Ala Trp Leu
965 970 975

Thr Ala Thr Ala Ala Arg Asn Ala Leu Val Leu Val Arg Gly Lys Arg
980 985 990

Leu Asp Gln Leu Pro Thr Pro Gly Pro His Leu Ala Gln Val Ala Gly
995 1000 1005

Ala Ser Gly Trp Asp Pro Asn Glu Tyr Gln Glu Tyr Leu Glu Asn
1010 1015 1020

Tyr Leu Lys Val Thr Arg Lys Ser Arg Gln Val Val Asp Glu Val
1025 1030 1035

Phe Trp Gly Val Asp Ser Met Glu Gln Arg Glu Phe
1040 1045 1050



5
29
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


5

ggggtcgacg gatcgacagg taatgcatt 29



6
29
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


6

ggggtcgacg gatccaccat gatggagga 29



7
22
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


7

cttcccagta gcaccatacg ac 22



8
26
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


8

ctggtggcag ttcgaagagg tccttg 26



9
26
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


9

ggacaaggac ctcttcgaac tgccag 26



10
26
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


10

cggcgagacc gtcgattggg aggagc 26



11
22
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


11

gtagcacctt acgaccaaac cg 22



12
20
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


12

ggagccggtc gacgaggagc 20



13
25
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


13

gctagcctcg ggagctctct aggag 25



14
25
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


14

gatctttccc agactctggc cacgc 25



15
17
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


15

cagttgtggc tgatccg 17



16
18
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


16

ctttcccaga ctctggcc 18



17
22
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


17

cgctgctata attgaacgtg ag 22



18
44
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


18

ctttgttgcc atatctgtgc gacgctgcta taattgaacg tgag 44



19
21
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


19

ccaccacgaa gtcggtggcg g 21



20
21
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


20

ttggagcctc gaagcctgga a 21



21
29
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


21

tggtcatatc tgtgcgacgc tgccataat 29



22
29
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


22

tggtcatatc tgtgcgacgc tgctataat 29



23
29
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


23

ttgccatatc tgtgcgacgc tgctataat 29



24
23
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


24

agacctacga gtccgccttt ttg 23



25
21
DNA
ARTIFICIAL SEQUENCE

SYNTHETIC DNA


25

cgatcaccag caacccacgc a 21