(en)Methods and compositions are provided for translational profiling and molecular phenotyping of specific tissues, cells and cell subtypes of interest. The methods provided herein facilitate the analysis of gene expression in the selected subset present within a heterogeneous sample.

1.ApplicationNumber: US-201715720658-A
1.PublishNumber: US-2018155728-A1
2.Date Publish: 20180607
3.Inventor: Heintz, Nathanial
GREENGARD, PAUL
HEIMAN, MYRIAM
SCHAEFER, ANNE
DOYLE, JOSEPH P.
DOUGHERTY, JOSEPH D.

4.Inventor Harmonized: HEINTZ NATHANIAL(US)
GREENGARD PAUL(US)
HEIMAN MYRIAM(US)
SCHAEFER ANNE(US)
DOYLE JOSEPH P(US)
DOUGHERTY JOSEPH D(US)

5.Country: US
6.Claims:
(en)Methods and compositions are provided for translational profiling and molecular phenotyping of specific tissues, cells and cell subtypes of interest. The methods provided herein facilitate the analysis of gene expression in the selected subset present within a heterogeneous sample.

7.Description:
(en)CROSS-REFERENCE
This application is a divisional of U.S. application Ser. No. 14/553,937, filed Nov. 25, 2014, and issued as U.S. Pat. No. 9,816,096 on Nov. 14, 2017, which is a continuation of U.S. application Ser. No. 12/918,761, filed Nov. 15, 2010, which is a national stage entry of International Application No. PCT/US09/01614, filed Mar. 12, 2009, which claims the benefit of U.S. Provisional Application Nos. 61/036,049 filed Mar. 12, 2008, 61/036,058 filed Mar. 12, 2008, 61/070,327, filed Mar. 21, 2008, and 61/199,108 filed Nov. 12, 2008, each of which applications is hereby incorporated by reference in its entirety.


STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
This invention was made with the support of the U.S. Government under Grant No: AG09464 awarded by the National Institute on Aging, MH074866 awarded by the National Institute of Mental Health, DA10044 and 5F32DA021487 awarded by the National Institute on Drug Abuse, 5UL1RR024143 awarded by the National Institutes of Health/National Center for Research Resources, and NS34696 awarded by the National Institute of Neurological Disorders and Stroke. The U.S. Government may have certain rights to the subject matter provided herein.


BACKGROUND OF THE INVENTION
A paradigm in the development of new diagnostics and therapies for human diseases and disorders is the characterization of the gene expression of defined cell types. The cellular complexity of many tissues poses a challenge for those seeking to characterize gene expression at this level. The enormous heterogeneity of a tissue such as the nervous system (thousands of neuronal cell types, with non-neuronal cells outnumbering neuronal cells by an order of magnitude) is a barrier to the identification and analysis of gene transcripts present in individual cell types. Cellular subtypes are highly heterogeneous and often intermixed. Gene expression studies on isolated cells have been limited by stresses introduced during cellular isolation procedures, the adaptations which occur upon the loss of tissue-intrinsic signals that control cellular physiology in vivo, and the technical challenges associated with reproducible mRNA purification from fixed tissue. A method to isolate translated mRNAs from defined cell types and subtypes without the need for cell isolation is needed in order to define previously undefined cell types, identify molecular targets for diseases and disorders, and provide a way in which to identify co-regulated gene sets for a particular biological function. The invention is described here.
INCORPORATION BY REFERENCE
All publications, patents, and patent applications mentioned in this specification are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated by reference.
SUMMARY OF THE INVENTION
The invention described herein provides methods, compositions, and kits useful in elucidating the biological properties of distinct cellular populations. The embodiments described herein comprise a translating ribosome affinity purification (TRAP) methodology, which is a generalizable method useful for the identification of molecular changes in any genetically defined cell type in response to genetic alterations, disease, environmental, pharmacological, other perturbations.
In one aspect, the invention described herein provides a method of identifying a co-regulated gene set for a function, comprising determining translational profiles for a plurality of cell types that express a gene associated with that function, comparing the translational profiles to determine what additional genes are similarly regulated, thereby identifying a co-regulated gene set involved in that function specifically. In one embodiment, the method is provided to identify a co-regulated gene set involved in myelination, and in a further embodiment the gene associated with myelination is Mbp. In one embodiment, the co-regulated gene set comprises at least two genes listed in Table 9. In a related embodiment, the gene set comprises at least two genes selected from the group consisting of Plp1, Cnp, Mog, Mal and Mobp.
In another aspect, the invention described herein provides a method for detecting one or more cell type-enriched genes not detectable by the whole-tissue microarray technique comprising assessing a translational profile for a cell type, comparing the translational profile to the results of a whole-tissue microarray, and determining the presence of one or more genes in the translational profile that are not present in the results of a whole-tissue microarray, thereby detecting one or more cell type-enriched genes that are not detectable by the whole-tissue microarray technique. In particular embodiments, greater than 20%, 30% or 40% of the genes enriched in that cell type are not detectable by the whole-tissue microarray technique. These methods can be applied to any cell type of interest including neuronal and non-neuronal cell types. In exemplary embodiments, the cell type is selected from the group consisting of striatal cell, cerebellar cell, cortical cell, hypothalamic cell, hippocampal cell, brainstem cell, and a spinal cord cell.
In another aspect, the invention described herein provides a method of identifying medium spiny neuron-enriched mRNAs, the method comprising expressing a ribosomal protein regulated by a regulatory region specific to a gene expressed in medium spiny neurons in an organism, isolating complexes comprising the ribosomal protein associated with mRNAs, identifying the mRNAs in the complexes, and comparing the mRNAs to those identified from a reference sample. In one embodiment the regulatory region used for expression is specific to a gene expressed in striatonigral neurons or striatopallidal neurons. In some embodiments the medium spiny neuron is associated with a disease or disorder such as but not limited to Parkinson's disease, addiction, attention deficit hyperactivity disorder, or Huntington's disease. In an additional embodiment, the method can be used in guiding the selection of candidate targets for the treatment of the disease or disorder, and for further screening of potential modulators of the candidate targets.
In yet another aspect, the invention described herein provides a method of identifying motor neuron-enriched mRNAs, the method comprising expressing a ribosomal protein regulated by a regulatory region specific to a gene expressed in motor neurons, isolating complexes comprising the ribosomal protein associated with mRNAs, identifying the mRNAs in the complexes, and comparing the mRNAs to those identified from a reference sample. In a specific embodiment, the regulatory region comprises regulatory sequences from a choline acetyltransferase (Chat) locus. This method can be utilized to identify mRNAS from any motor neuron, such as but not limited to brain stem motor neurons, spinal cord motor neurons, upper motor neurons and others.
In another aspect, the invention described herein provides a method for assessing the translational profile of a cerebellar cell, comprising expressing a ribosomal protein regulated by a regulatory region specific to a cerebellar gene in the cell, isolating at least one complex comprising the ribosomal protein associated with a mRNA from the cell, and identifying the mRNA from the complex whereby generating a translational profile. In one embodiment, the cerebellar cell is associated with a disease or disorder, such as ataxia.
The present invention also provides for kits comprising a recombinant vector engineered to express a nucleic acid sequence encoding a ribosomal protein and a detectable tag operably linked to an endogenous regulatory region, useful for practicing the methods described herein.



BRIEF DESCRIPTION OF THE DRAWINGS
The novel features of the invention are set forth with particularity in the appended claims A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:
FIG. 1 illustrates a CNS disease-related BACarray collection.
FIG. 2 presents the translating ribosome affinity purification (TRAP) methodology. (a) Schematic of affinity purification of eGFP tagged polysomes (originating from the target cell population) using anti GFP antibody coated beads. (b) Transmission electron micrographs of anti GFP coated magnetic beads after incubation with extracts taken from HEK293T cells transfected with an empty vector (left panel) or the eGFP L10a construct (right panel); images acquired at 50,000× magnification, inserts enlarged by a factor of 2.3×.
FIG. 3 illustrates immunoprecipitation of translated mRNAs from transfected cells.
FIG. 4 illustrates the in vivo BACarray strategy.
FIG. 5 illustrates BAC vector engineering.
FIG. 6 illustrates BACarray mouse generation.
FIG. 7 illustrates examples of drivers used to produce BACarray mice with specific expression in the cerebral cortex.
FIG. 8 illustrates examples of drivers used to produce BACarray mice with specific expression in the hypothalamus
FIG. 9 Panels 1-25 illustrates that BAC transgenesis targets eGFP-L10a to specific CNS cell populations. DAB immunohistochemistry with anti-eGFP antibody on each mouse line reveals a unique and specific pattern of expression for the eGFP-L10a transgene. Panels (10x) show the morphology and localization of cell types expressing the transgene, while inset shows location of panel, for cerebellar (1-9), spinal cord (10), striatal/basal forebrain (11-14) brainstem (15), and cortical (16-25) cell types. Dashed lines (panels 16-25) indicate corpus callosum. A key for all cell types is in FIG. 11A .
FIG. 10 illustrates that GENSTAT eGFP and BACarray cortical pyramidal cell lines have same laminar distribution. A) Graph (mean+/−SE) of the distance of eGFP+cell somas from the pial surface for the BACarray and GENSAT eGFP lines for each pyramidal cell BAC driver. The depth of the cells was consistent between both lines for each driver. B) The percentage of cells in 100 micron bins is shown as a histogram of the distribution of cell depths for each line in A. The BACarray line and eGFP line for each driver had overlapping distributions of cell depths.
FIG. 11 illustrates a summary of cell types studied and characterization of lines. A) Table of all cell populations studied with BACarray, corresponding to FIG. 9 . B) Detection of eGFP-L10a under the control of a Pcp2 BAC in Calbindin positive Purkinje cells. C) Detection of eGFP-L10a under the control of a Neurod1 BAC in granule cells with NeuN positive nuclei. D) Detection of eGFPL10a under the control of a Lypd6 BAC in Parvalbumin positive outer stellate and deep stellate (basket) neurons of the molecular layer, but not in Parvalbumin positive Purkinje cell fibers (arrow). E) Detection of eGFP-L10a under the control of a Grm2 BAC in Grm2/3 positive interneurons (Golgi cells) of the granule cell layer, but not in Grm2/3 positive glomeruli (arrow). F) eGFP-L10a detected under the control of a Grp BAC in unipolar brush cells with Grml positive brushes (arrow). G) eGFP-L10a detected under the control of a Sept4 BAC in s100 positive Bergman glia.
FIG. 12 illustrates that higher transgene expression yields better signal to noise. A) DAB immunohistochemistry for eGFP-L10a reveals differential intensity of transgene expression in two BACarray lines targeting the same cell population. B) Quality of BACarray data, as assessed by average IP/UB fold change for positive and negative controls, is better for the line with higher transgene expression.
FIG. 13 illustrates protein and mRNA purification from BACarray lines. (a) Purification of eGFP tagged L10a and co purification of untagged ribosomal protein L7 from D1 BACarray animals but not wild type littermates (D1, samples from D1 BACarray mice; WT, samples from wild type littermates; In, 1% Input; UB, 1% Unbound; IP, 6.5% Immunoaffinity purified sample). eGFP L10a signal is only present in the D1 IP lane because the IP samples were more concentrated relative to In and UB. (b) Purification of 18 S and 28 S rRNA from D1 BACarray transgenic animals (top panel) but not wild type littermates (bottom panel) as detected by Bioanalyzer PicoChips (Agilent Technologies). 28 S rRNA runs at ˜47 sec, 18 S rRNA runs at ˜43 sec, and the marker peak runs at ˜23 sec.
FIG. 14 illustrates electron microscopy of immunoprecipitated ribosome complexes.
FIG. 15 illustrates that microarray signal intensity does not increase with transcript length. A-DD: To assess whether longer mRNA's were more efficiently immunoprecipitated than shorter transcripts, transcript length (Y-axis, log base 2), was plotted versus GCRMA normalized signal intensity (X-axis, log base 2) for all 30 samples. None of the immunoprecipitated nor the unbound (whole tissue RNA) samples show positive correlations between length and signal.
FIG. 16 illustrates that BACarray can consistently purify RNA efficiently from rare and common cell types. A) Western blot for eGFP from S20 fraction, flow through (UB), and immunoprecipitate from rare (unipolar brush cells), common (Purkinje cells) and extremely common (granule cells) cell types of cerebellum. Note that even when protein is undetectable, there is sufficient good quality RNA for microarray amplification. B) Yield of RNA is consistent across replicates within each line. C) Total RNA is of good quality for all three cell types, with intact 18s and 28s bands, as well as mRNA which can be amplified and fragmented following standard protocols.
FIG. 17 illustrates that BACarray data are reproducible and cell-type specific.
FIG. 18 illustrates that BACarrays can identify known markers, and discover new ones for a variety of cell types.
FIG. 19 illustrates that BACarray clusters cells by type and provides greater sensitivity than whole tissue arrays.
FIG. 20 illustrates cell type diversity is driven by proteins on the cell surface, such as receptors and ion channels.
FIG. 21 illustrates that a comparative analysis reveals unique translational profiles for each cell type.
FIG. 22 illustrates the transcriptional sketch of a spinal motor neuron.
FIG. 23 illustrates expression of eGFP-L10a in the Chat and Purkinje cell BACarray lines. (A) Immunohistochemistry to eGFP in adult sagittal sections from the Chat BACarray line DW167. (B) Indirect immunofluorescent characterization of Chat BACarray line DW167 brain stem facial motor nucleus: eGFP staining (left panel); Chat staining (middle panel) and merge (right panel, with 20 um scale bar). (C) Immunohistochemistry to eGFP in adult sagittal sections from the Pcp2 BACarray line DR166. (D) Indirect immunofluorescent characterization of Pcp2 BACarray line DR 166 Purkinje cell neurons: eGFP staining (left panel); Calbindin-D28K staining (middle panel) and merge (right panel, with 20 um scale bar).
FIG. 24 illustrates that BACarray profiles recapitulate known cell-specific markers and reveal new ones for four distinct cell types. Scatterplots of D1, D2, Chat, and Pcp2 BACarray data compared to a reference mRNA sample reveal hundreds of genes enriched in each cell type (A-D). Lines on either side of the diagonal mark 2-fold enrichment. Axes are labeled for expression in powers of 10. Venn diagrams of the top 1,000 enriched probesets (Tables 17-20) (with expression value cut-off >100) for each cell type reveal that each cell type has a unique pattern of enriched genes (E-H).
FIG. 25 illustrates expression of eGFP L10a in D1 and D2 BACarray lines. (a) Immunohistochemistry to eGFP in adult sagittal sections from the D2 BACarray line CP101. (b) Characterization of D2 BACarray line CP101 striatal MSN cells: direct eGFP fluorescence (left panel with high magnification image insert); enkephalin immunohistochemical staining (middle panel); merge (right panel, with 20 μm scale bar). (c) Immunohistochemistry to eGFP in adult sagittal sections from the D1 BACarray line CP73. (d) Characterization of D1 BACarray line CP73 striatal MSN cells: direct eGFP fluorescence (left panel); enkephalin immunohistochemical staining (middle panel); merge (right panel).
FIG. 26 illustrates the polysome profile from D2 BACarray mouse striatal extracts.
FIG. 27 illustrates an analysis of striatal MSN IP values relative to message abundance and length. Lengths of transcripts were based on all available mouse curated RefSeq RNA sequences (ftp://ftp.ncbi.nih.gov/genomes/M musculus/RNA). Where multiple transcript variants for a single gene were available, the longest one was chosen. RefSeq lengths were plotted against D1 (a) or D2 (b) BACarray IP normalized expression values. There was no correlation observed between transcript length and IP values. Striatal expression values for all Affymetrix Genechip probe sets were obtained by total RNA arrays from wild type striatal tissue (data not shown). These values were plotted against (c) D1 BACarray or (d) D2 BACarray IP normalized values. As expected, higher expression in total striatum (no IP, wild type mice) correlates with higher D1 or D2 BACarray IP values. The few genes that show modest expression in total striatum but have low IP values include known non neuronal genes.
FIG. 28 illustrates gene expression analysis of BACarray purified mRNA. Normalized expression values from Affymetrix Mouse Genome 430 2.0 arrays are plotted for D1 and D2 BACarray samples. Middle diagonal line represents equal expression, and lines to each side represent 1.5 fold enrichment in either cell population.
FIG. 29 illustrates expression analysis of medium spiny neuron (MSN) enriched genes. Expression analysis in sagittal sections of genes which were amongst the top 100 (a) or 1,000 1,100 (b) genes identified in the study as MSN enriched, with the rank order of each gene noted below the gene name. Non redundant gene ranking was calculated using the highest ranked probeset corresponding to each gene with redundant probesets eliminated. Left panel, in situ hybridization images taken from the Allen Brain Atlas (Allen Brain Atlas [Internet]. Seattle (Wash.): Allen Institute for Brain Science. ©2006. Available from: http://www.brain map.org.) (3); right panel, in situ hybridization images taken from the Brain Gene Expression Map (BGEM) database (http://www.stjudebgem.org/) (4). Allen Brain Atlas images all correspond to adult brain; BGEM images all correspond to adult brain except for the following, for which the oldest available data were postnatal day 7 (P7): Drd2, Ppp1r1b, Dlx6, Gdnf, Bc111b, Foxg1, Limd2, Fem1b, Dyn111, Atbf1, Foxo3a, Dnalc4, Mtmr7, Dnmt3a.
FIG. 30 illustrates that functional Gpr6 receptors are found in BAC D2 striatopallidal neurons but not BAC D1 striatonigral medium spiny neurons. (a) Projection of an eGFP labeled medium spiny neuron from a BAC D2 mouse. The cell was patched with a pipette containing Alexa 594 (50 μM) for visualization and Fluo 4 (200 μM) for measuring changes in intracellular Ca2+ (right). Cells were voltage clamped at −70 mV. A puffer pipette containing sphingosine 1 phosphate (S1P, 10 μM) was positioned near a dendrite, 60 80 μm from the soma (left/cartoon). (b) High magnification images of a dendritic segment (control, left panel) show an increase in Ca2+ associated with S1P application (S1P puff, center panel) that reversed with washing (wash, right panel). The change in Ca2+ was determined by calculating the percent change in fluorescence of Fluo 4 relative to that of Alexa 594 (ΔG/R). (c) Time course showing the S1P induced increase in intracellular Ca2+ in the ROI from b (orange trace); similar recordings from BAC D1 medium spiny neurons (black trace) or thapsigargin loaded BAC D2 medium spiny neurons did not reveal any changes in dendritic Ca2+ levels with S1P application. (d) Box plot summarizing the S1P effects. Percent increase in fluorescence (ΔG/R) in BAC D2 medium spiny neurons (median=146%, range 44 to 294%, n=6); BAC D1 medium spiny neurons (median=17%, range 13 to 22%, n=4); and thapsigargin loaded BAC D2 medium spiny neurons (median=4%, range −9 to 10%, n=4).
FIG. 31 illustrates that cocaine treatment increases the frequency of small amplitude GABAergic mIPSCs in BAC D1 striatonigral neurons. (a) Representative spontaneous mIPSCs traces from BAC D1 striatonigral neurons (expressing soluble eGFP under the D1 promoter) taken from mice treated for 15 days with saline or (b) cocaine (20 mg/kg/day). (c) Bar graph summary of mean mIPSC frequency showing a increase in BAC D1 striatonigral neuron mIPSCs frequency following cocaine treatment (Mann Whitney Rank Sum Test, p<0.05, saline median=0.82 Hz, n=22; cocaine median=1.03 Hz, n=26). (d) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) increased in BAC D1 striatonigral neurons following cocaine treatment (t test, p<0.05, saline=79.4±8.2, n=22; cocaine=104.2±6.3, n=26). (e) Representative variance mean current plots from saline treated and cocaine treated BAC D1 neurons suggesting that the cocaine induced small amplitude events arise from synapses that have fewer GABAA receptors (N) per synapse but receptors with an unchanged unitary receptor conductance (g) (saline N=33, g=31 pS; cocaine N=27, g=30 pS; see FIG. S6c d for means). (f) Representative spontaneous mIPSCs traces from BAC D2 striatopallidal neurons following saline treatment for 15 days and (g) following cocaine treatment for 15 days. (h) Bar graph summary of mean mIPSC frequency in saline and cocaine treated neurons, showing no effect of treatment condition (t test, p>0.05, saline=0.72±0.05 Hz, n=12; cocaine=0.78±0.07 Hz, n=16). (i) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) was not altered by treatment condition in BAC D2 neurons (t test, p>0.05, saline=68.1±9.7, n=12; cocaine=71.7±7.5, n=16). (j) Representative variance mean current plots showing that cocaine treatment did not change in the number of receptors per synapse or the unitary receptor conductance in BAC D2 neurons (saline N=29, g=33pS; cocaine N=31, g=29pS; ).
FIG. 32 illustrates that cocaine treatment decreases the number of GABAA channels per synapse in BAC D1 striatonigral neurons. Box plot summaries showing mean mIPSCs kinetics and non stationary noise analysis measures of individual striatonigral neurons taken from saline treated control and 15 day treated cocaine BAC D1 mice. (a) Neither mean 10 90% rise times (t test, p>0.05, Saline=0.64±0.008, n=22; Cocaine=0.65±0.006, n=26) nor (b) mean decay times (ttest, p>0.05, Saline=17.3±0.5, n=22; Cocaine=17.0±0.5, n=26) were different between groups. Non stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (g) of GABAA receptors of striatonigral neurons (t test, p>0.05, Saline g=31.1±1.6, n=22; Cocaine g=30.8±1.2, n=26), and a decrease in the (d) mean number of channels per synapse in striatonigral neurons taken from cocaine treated BAC D1 mice (t test, p<0.05, Saline N=33.1±1.4, n=22; Cocaine N=29.3±1.0, n=26).
FIG. 33 illustrates that cocaine treatment does not alter GABAergic mIPSCs kinetics or GABAA receptor number per synapse or unitary receptor conductance in BAC D2 striatopallidal neurons. Box plot summaries showing mean mIPSCs kinetics and non stationary noise analysis measures of individual striatopallidal neurons taken from saline treated control and 15 day treated cocaine BAC D2. (a) Neither mean 10 90% rise times (t test, p>0.05, Saline=0.65±0.007, n=12; Cocaine=0.64±0.010, n=16) nor (b) mean decay times (t test, p>0.05, Saline=18.0±1.0, n=12; Cocaine=18.0±0.5, n=16) were different between groups. Non stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (referred to as g in the y-axis) of GABAA receptors of striatopallidal neurons (t test, p>0.05, Saline g=30.7±1.5, n=12; Cocaine g=30.3±1.2, n=16) or in the (d) mean number of channels per synapse in striatopallidal neurons taken from cocaine treated BAC D2 mice (t test, p>0.05, Saline N=33.8±2.7, n=12; Cocaine N=33.0±1.6, n=16).
FIG. 34 illustrates BACarray molecular phenotyping of cerebellar cell types in ATM KO mice.
FIG. 35 illustrates the characterization of Pcp2 and Sept4 BACarray transgenic mouse lines. (A) Expression of the eGFP L10a fusion in Purkinje cells in the cerebellum of the Pcp2 BACarray line. Immunohistochemical stain of Pcp2 BACarray brain section using anti eGFP antiserum (top panel). Double immuno fluorescence analysis (bottom panels) of calbindin (Calb1) and eGFP L10a expression in Pcp2 BACarray mice showing co labeling of Purkinje cells (merge). (B) Expression of the eGFP L10a fusion in Bergmann glia in the cerebellum of the Sept4 BACarray line. Immunohistochemical stain of Sept4 BACarray brain section using anti eGFP antiserum (top panel). Double immuno fluorescence analysis of glutamine synthetase (Glns) and eGFP L10a expression in Sept4 BACarray mice showing co labeling of Bergmann glial cells (merge).
FIG. 36 illustrates a scatter Plot analysis of BACarray data for Septin4 and Pcp2 lines. Immunoprecipitated (IP) mRNA from Septin4 (A, C) and Pcp2 (B, D) BAC transgenic lines was directly compared in scatter plot analysis to mRNA samples from total cerebellum. The analysis clearly demonstrates that thousands of genes are enriched in the immunoprecipitated samples relative to total cerebellum. Scatter plots (C) and (D) show the same experiments with known positive control genes for Bergmann glial cells and Purkinje neurons. Bergmann glial positive controls are clearly enriched in the Septin4 IP samples (C) whereas Purkinje cell positive control genes are enriched in Pcp2 IP samples (D).
FIG. 37 illustrates cell type specific differential regulation of gene expression in Atm−/− cerebellum. (A-C) Atm−/− IP data were compared to Atm+/+IP data in scatter plot analyses for the Septin4 (A), the Pcp2 (B) and the total cerebellum (C) experiments. Gene lists of both upregulated and downregulated genes were generated by filtering on expression values greater than 25, on fold change values greater than 1.5, and performing one way ANOVA, p=0.05. Scatter plots display the distribution of regulated genes in each experiment. Colors refer to the Venn Diagram analysis (D). Genes shared by Bergmann glia and Purkinje cells are colored yellow, those shared by Purkinje cells and total cerebellum are turquoise, and those shared by Bergmann glia and total cerebellum are colored magenta. Genes identified in all three conditions are colored white. (D) Venn Diagram analysis of all regulated genes. In total cerebellum, 40/69 genes are found differentially regulated exclusively in total cerebellum, in the Septin4 experiment, 94/116 genes are specifically regulated in the Septin4 data, and in the Pcp2 experiment, 62/89 regulated genes which are specific to the Pcp2 data have been identified.
FIG. 38 illustrates ventral tegmental area and substantia nigra area specific markers, as revealed by BACarrays.
FIG. 39 illustrates dopaminergic cell loss in the rodent substantia nigra with the use of 6-OHDA.
FIG. 40 illustrates a therapeutic opportunity for Parkinson's disease, by developing new strategies for stimulation of Sub-Thalamic Nucleus, by using antagonists of Gpr6.



DETAILED DESCRIPTION OF THE INVENTION
Translational Profiling and Molecular Phenotyping
The present disclosure provides for methods and compositions useful in translational profiling and molecular phenotyping of heterogeneous tissues and cell types. In one embodiment the methods can be used to define previously undefined cell types, identify molecular targets for diseases and disorders, and provide a way in which to identify co-regulated gene sets for particular biological and/or medically relevant functions.
Translational profiling is the profiling, identification, or isolation of translated mRNAs. In some embodiments such profiling is a measure of the nascent proteome. In other embodiments, this profiling allows for the identification of mRNAs being actively translated, or otherwise associated with the cellular translational machinery. Molecular phenotyping is the molecular and/or gene expression description of organs, tissues, and cell types.
The present disclosure provides for methods and compositions to practice translating ribosome affinity purification (TRAP) profiling methodology. In one embodiment these profiling methods are be utilized to further distinguish morphologically, anatomically, developmentally, or otherwise indistinguishable, cells into cellular subtypes, further defining cell populations and sub-populations. In some cases, these otherwise indistinguishable cells are intermixed. In other cases, these cells are spatially separated. In some cases, these cells are cells of the central or peripheral nervous system, for example neurons or glia, only distinguishable by their translational profiles and molecular phenotypes. In other cases these are cells outside the nervous system. In another embodiment these profiling methods are be utilized to identify translated mRNAs in single cell types with a sensitivity that is may not be achievable by techniques such as whole-tissue microarray. In yet other embodiments, these profiling methods are used to identify co-regulated gene sets for particular biological functions in cells that are spatially separated or intermixed, morphologically distinct or indistinct, in different developmental stages, associated with different tissues or regions of a tissue, or from different individuals with the same disease, disorder or state.
The methods provided herein allow for isolation of mRNAs associated with ribosomes or polysomes (clusters of ribosomes) from specific cell types, allowing for cell translational profiling and molecular phenotyping of the cell types and subtypes. In some cases, the cells are genetically targeted.
The methods described herein in allow for identifying translated mRNAs in any cell subtype of interest. The methodology involves expression of a tagged functional ribosomal protein, which enables tagging of polysomes for purification of mRNA, in specific cell populations. In some embodiments, the purification of polysomes is by affinity or immunoaffinity purification. In some embodiments, the cell subtypes are genetically targeted. In some embodiments the tagged ribosomes are expressed in transgenic animals
The translating ribosome affinity purification methodology (TRAP) for translational profiling and molecular phenotyping involves expression of a tagged ribosomal protein, which enables tagging of polysomes for affinity-based, sometimes immunoaffinity-based, purification of mRNA, in specific cell populations. In some cases this is achieved with the use of animals or transgenic animals, allowing translation profiling and molecular phenotyping from whole animals Methods provided herein allow for defining the distinguishing molecular characteristics of closely related or critical cell types. Methods also demonstrate that methods described herein can be employed to analyze physiological adaptations of specific cell types in vitro, in vivo, ex vivo, or in situ.
Molecular Tagging of Ribosomes
Embodiments of this invention provide methods for isolating cell type- or cell subtype-specific polysomal, mRNA. The methods include using molecularly tagged ribosomal proteins resulting in functional tagged ribosomes or ribosomal complexes, able to support translation, assemble into polysomal complexes. Ribosomes can be molecularly tagged and expressed in one or more cell types or cell subtypes of interest. Molecularly tagged ribosomal proteins for the purposes of this disclosure will interchangeably be referred to as a ‘fusion proteins’ or ‘tagged proteins’ or ‘molecularly tagged fusion proteins’ or ‘molecularly tagged ribosomal proteins’. In various embodiments these fusion proteins contain all or a portion of a ribosomal proteins. In certain embodiments ribosome tagging causes no disruption of native function or distribution of the tagged protein, thus resulting in a functional ribosome, functional ribosomal complex, or functional polysome. That is, although molecularly tagged, the portion that has the biological activity of the native ribosomal protein is retained and can function in an intact ribosome to carry out translation or binding of mRNA. In some embodiments, the molecularly tagged ribosomal is expressed in a organ, tissue, cell type, or cell subtype of interest, by introducing into cells, culture, slices, or into an entire organism, a nucleic acid encoding the molecularly tagged ribosomal protein under the control of regulatory elements or transcriptional units that direct expression in the cell type or cell subtype of choice. In some embodiments the ribosomal protein to be tagged can be from the same or different species as the cell that expresses the molecularly tagged protein.
In certain embodiments, the ribosome or ribosomal protein is molecularly tagged by engineering the ribosomal protein to fuse to or bind a small molecule, a protein, or peptide that is not bound by the unengineered ribosome or the ribosomal protein. In certain embodiments the nucleic acid encoding the ribosomal protein fused to the tag can be generated by routine genetic engineering methods in which a nucleotide sequence encoding the amino acid sequence for the tag sequence is engineered in frame with the nucleotide sequence encoding a ribosomal protein. This can be accomplished by any method known in the art, for example, via oligonucleotide-mediated site-directed mutagenesis or polymerase chain reaction (PCR) and other routine protocols of molecular biology (see, e.g., Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y., both of which are hereby incorporated by reference in their entireties).
In exemplary embodiments the ribosomal protein is L10a and the tag is eGFP.
1) Ribosomal Proteins
Nucleic acids encoding the tagged proteins can be produced using genetic engineering methods and cloning and expression vectors that are well known in the art. In some embodiments, a naturally occurring or synthetically produced nucleic acid or gene can be engineered to function as a ribosomal protein and support translation or bind (directly or indirectly) or associate with polysomal, translating ribosomal mRNA. Nucleic acids encoding the ribosomal protein to be molecularly tagged may be obtained using any method known in the art. Nucleic acids may be obtained, for example, by PCR using oligonucleotide primers based upon the published sequences. Other related ribosomal (for example from other species) may be obtained by low, medium or high stringency hybridization of appropriate nucleic acid libraries using the ribosomal in hand as a probe.
Exemplary ribosomal proteins for use in various embodiments of this invention are provided in
Table 1, but not limited to those listed. In an exemplary embodiment, the ribosomal protein is L10a. In some embodiments the tagged ribosomal protein is incorporated into a ribosomal complex and is associated with one or more mRNAS, but does not bind mRNA directly.





TABLE 1



Ribosomal Proteins













A52
L11
L23a
L35a
LP2 (Large P2)
S11
S24

Ke-3
L12
L24
L36
LP1 (Large P1)
S12
S25

L3
L13
L26
L36a
S2
S13
S26

L3L (L3-like)
L13a
L27
L37
S3
S14
S27

L4
L14
L27a
L37a
S3a
S15
S27a

L5
L15
L28
L38
S4
S15a
S28

L6
L17
L29
L39
S5
S16
S29

L7
L18
L30
L41
S6
S17
S30

L7a
L18a
L31
L44
S7
S18
S23

L8
L19
L32
LAMR1
S8
S19
RPLP1

L9
L21
L32-3a (3a)
LLRep3
S9
S20

L10
L22
L34
LP0 (Large P0)
S10
S21







L10a
L23
L35
Region containing





hypothetical protein FLJ23544





































In certain embodiments, a nucleic acid encoding a molecularly tagged ribosomal protein is intended for a particular expression system, in which the codon frequencies reflect the tRNA frequencies of the host cell or organism in which the protein is expressed. Codon optimization can allow for maximum protein expression by increasing the translational efficiency of a gene of interest. In other embodiments the nucleic acid encoding a molecularly tagged ribosomal protein may be a synthetic nucleic acid in which the codons have been optimized for increased expression in the host cell in which it is produced.
2) Molecular Tags
Molecular tags can be any protein (or fragment, portion, analog or derivative thereof) that is not present or accessible in the cell of interest (or the cell fraction from which the tagged ribosomes are to be isolated, or other cells that will be contacted with the reagent that binds the tag) for which there exists a reagent (such as an antibody) or method (such as optical, fluorescence or magnetic sorting) that recognizes the tag and that is accessible to solution (and thereby, the tag).
Molecular tagging with tags that are traditionally utilized as reporter genes is well known in the art (Current Protocols in Molecular biology, Section 9.6.1, “Uses of Fusion Genes in mammalian Transfection” (2004)). Molecular tagging with epitopes are also well known in the art (“epitope tagging” reviewed in Fritze C E, Anderson T R. Epitope tagging: general method for tracking recombinant proteins. Methods Enzymol. 2000; 327:3-16; Jarvik J W, Telmer C A. Epitope tagging. Annu Rev Genet. 1998; 32:601-18).
Tags can include those for which methods/reagents/devices exist that allow facile identification and isolation of the tagged protein, but do not, minimally, or negligibly inhibit or interfere with function of the tagged protein. The tag may be an intact naturally occurring or synthetically produced protein, fragment, analog or derivative thereof of any length that permits binding to the corresponding binding reagent/method. In certain embodiments, the tag is about 8, 10, 12, 15, 18 or 20 amino acids, is less than 15, 20, 25, 30, 40 or 50 amino acids, but may be 100, 150, 200, 250, 300, 400, 500, 1000 or more amino acids in length. The tag may be bound specifically by a reagent that does not otherwise bind any component of: (1) the cell of interest; or (2) a polysomal preparation of interest; or (3) whatever cellular fraction of interest is being contacted by the reagent that binds the tag.
In certain embodiments, the nucleotide sequence encoding a tag is preferably inserted in frame such that the tag is placed at the N- or C-terminus of the ribosomal protein, since these portions of proteins are often accessible to detection, affinity, immunological, or purification reagents. Alternatively, the tag can be inserted into any portion of the ribosomal protein such that when the fusion protein is incorporated into an intact ribosome, ribosomal function is not compromised and the tag is accessible to the reagent/method to be used in the detection, isolation or and/or purification. That is, tagging can still allow for a functional ribosome. In other embodiments, a ribosomal protein may be molecularly tagged with a plurality of tags.
In some embodiments, the tag is Green Fluorescent Protein (GFP). GFP can be utilized as an optical or immunoaffinity tag for a variety of applications, all well known in the art. GFP is a protein, composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish that fluoresces green when exposed to blue light (Prendergast F, Maim K (1978). “Chemical and physical properties of aequorin and the green fluorescent protein isolated from Aequorea forskålea”. Biochemistry 17 (17):3448-53; Tsien R (1998). “The green fluorescent protein”. Annu Rev Biochem 67: 509-44). The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm which is in the lower green portion of the visible spectrum. The GFP from the sea pansy ( Renilla reniformis ) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression (Phillips G (2001). “Green fluorescent protein—a bright idea for the study of bacterial protein localization”. FEMS Microbiol Lett 204 (1): 9-18.). In modified forms it has been used to make biosensors, and many animals have been created that express GFP. The GFP gene can be introduced into organisms and maintained in their genome through breeding, or local injection with a viral vector can be used to introduce the gene. To date, many bacteria, yeast and other fungal cells, plant, fly, and mammalian cells have been created using GFP as a marker.
In other embodiments, the tag is a GFP mutant, variant, analog, fragment, or derivative thereof. Different mutants of GFP have been engineered (Shaner N, Steinbach P, Tsien R (2005). “A guide to choosing fluorescent proteins”. Nat Methods 2 (12): 905-9). One improvement was a single point mutation (S65T) reported in 1995 in Nature by Roger Tsien (Heim R, Cubitt A, Tsien R (1995). “Improved green fluorescence”. Nature 373 (6516): 663-4.)). The addition of the 37° C. folding efficiency (F64L) point mutant to the scaffold yielded enhanced GFP (eGFP). eGFP has an extinction coefficient (denoted c), also known as its optical cross section of 9.13×10-21 m 2 /molecule, also quoted as 55,000 L/(mol·cm) (Shelley R. McRae, Christopher L. Brown and Gillian R Bushell (May 2005)). “Rapid purification of eGFP, EYFP, and ECFP with high yield and purity”. Protein Expression and Purification 41 (1): 121-127.). Superfolder GFP, a series of mutations that allow GFP to rapidly fold and mature even when fused to poorly folding peptides, was reported in 2006 (Pédelacq J, Cabantous S, Tran T, Terwilliger T, Waldo G (2006). “Engineering and characterization of a superfolder green fluorescent protein”. Nat Biotechnol 24 (1): 79-88). In an exemplary embodiment the tag is enhanced GFP (eGFP).
Other mutations have been made, including color mutants; in particular blue fluorescent protein (EBFP, EBFP2, Azurite, mKalamal), cyan fluorescent protein (ECFP, Cerulean, CyPet) and yellow fluorescent protein derivatives (YFP, Citrin, Venus, YPet). BFP derivatives (except mKalamal) contain the Y66H substitution. The mutation in cyan derivatives is the Y66W substitution. The red-shifted wavelength of the YFP derivatives is accomplished by the T203Y mutation (Tsien R (1998). “The green fluorescent protein”. Annu Rev Biochem 67: 509-44).
Molecular tags may include both fluorescent, magnetic, epitope, radioactive or otherwise detectable tags, by way of example, and not by limitation, those listed in Table 2.





TABLE 2



Molecular Tags







GFP, eGFP, mGFP, emerald GFP, derivatives and variants (Green

Fluorescent protein)

BFP, eBFP, eBFP2, Azurite, mKalamal, sapphire GFP, derivatives and

variants (Blue fluorescent proteins)

ECFP, Cerulean, CyPet, DFP, TFP, mTFP, derivatives and variants

(cyan and teal fluorescent proteins)

YFP, Citrine, Venus, YPet, Topaz GFP, derivatives and variants

(yellow fluorescent proteins)

RFP, mCherry, td-tomato, derivatives and variants (red fluorescent

protein)

Bgal, derivatives and variants (Bgalactosdiase)

Luc, derivatives and variants (Luciferase)

CAT, derivatives and variants (Chloramphenicol acetyltransferase)

hGH, derivatives and variants (human growth hormone)

Protein A fragments;

Myc epitopes

Btag and polyhistidine tracts

Influenza virus hemagglutinin protein, fragments, derivatives, variants

C-myc gene, fragments, derivatives, variants

Bluetongue virus VP7 protein, fragments, derivatives, variants

FLAG peptide, fragments, derivatives, variants

Strep-tag peptide, fragments, derivatives, variants

pHlourins, fragment, derivatives, variants











































References for Table 2 are provided here, and incorporated by reference herein: Evan et al., Mol. Cell Biol. 5(12):3610-3616; Wang et al., 1996, Gene 169(1):53-58; Bornhorst et al., 2000, Purification of proteins using polyhistidine affinity tags, Methods Enzymol 326:245-54; Wilson I A, Niman H L, Houghten R A, Cherenson A R, Connolly M L, Lemer R A. The structure of an antigenic determinant in a protein. Cell. 1984 July; 37(3):767-78; Evan G I, Lewis G K, Ramsay G, Bishop J M. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol. 1985 December; 5(12): 3610-6; Wang L F, Yu M, White J R, Eaton B T. BTag: a novel six-residue epitope tag for surveillance and purification of recombinant proteins. Gene. 1996 Feb. 22; 169(1):53-8; Hopp et al., U.S. Pat. No. 4,703,004, entitled “Synthesis of protein with an identification peptide” issued Oct. 27, 1987; Brizzard B L, Chubet R G, Vizard D L. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques. 1994 April; 16(4):730-5; Knappik A, Pluckthun A. An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments. Biotechniques. 1994 October; 17(4):754-761; Skerra et al., U.S. Pat. No. 5,506,121, entitled Fusion peptides with binding activity for streptavidin, issued Apr. 9, 1996; Skerra A, Schmidt T G. Applications of a peptide ligand for streptavidin: the Strep-tag. Biomol Eng. 1999 Dec. 31; 16(1-4):79-86; Skerra A, Schnudt T G. Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. Methods Enzymol. 2000; 326:271-304; Shaner N, Steinbach P, Tsien R (2005). “A guide to choosing fluorescent proteins”. Nat Methods 2 (12): 905-9. doi:10.1038/nmeth819. PMID 16299475.; Shaner N, Steinbach P, Tsien R (2005). “A guide to choosing fluorescent proteins”. Nat Methods 2 (12): 905-9. PMID 16299475; Heim R, Cubitt A, Tsien R (1995). “Improved green fluorescence”. Nature 373 (6516): 663-4. PMID 7854443; Shelley R. McRae, Christopher L. Brown and Gillian R Bushell (May 2005). “Rapid purification of eGFP, EYFP, and ECFP with high yield and purity”. Protein Expression and Purification 41 (1): 121-127; Pédelacq J, Cabantous S, Tran T, Terwilliger T, Waldo G (2006). “Engineering and characterization of a superfolder green fluorescent protein”. Nat Biotechnol 24 (1): 79-88. PMID 16369541; Miesenbock G, De Angelis D, Rothman J (1998). “Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins”. Nature 394 (6689): 192-5. PMID 9671304.
Isolation Of Ribosomes, Polysomes, mRNA
1) Isolation of Ribosomes
Various methods exist to isolate ribosomes/tagged ribosomes, particularly polysomes (ribosomal clusters bound to mRNA)/tagged polysomes, from cells, cultured cells and tissues (see, e.g., Bommer et al., 1997, Isolation and characterization of eukaryotic polysomes, in Subcellular Fractionation, Graham and Rickwood (eds.), IRL Press, Oxford, pp. 280-285; incorporated herein by reference in its entirety). Polysomes are interchangeably referred to as polyribosomes, ribosomal complexes or ribosomal clusters. In preferred embodiments, the isolated polysomes (ribosomal-mRNA complexes) contain functional ribosomes, capable of supporting translation, association with mRNA, and/or association with translation factors.
In certain embodiments, the isolation method employed has one or more of the following aspects:
a. Maintenance of ribosomal subunits on mRNA during isolation: translation arresting compounds, such as emetine or cycloheximide can be added to arrest translation, whereby reducing or preventing dissociation of mRNA from the ribosome. In preferred embodiments isolation is achieved without crosslinking and crosslinking reagents; b. Inhibition of endogenous RNAase activity: RNAase inhibitors can be added to buffers to maintain the integrity of the mRNA; c. Isolation of Polysomes: After tissue or cell homogenization, total polysomes are isolated by preparing a post-mitochondrial supernatant in the presence of at least a high concentration salt buffer, for example about 100-150 mM KCl; and d. Solubilization of rough ER-bound Polysomes under non-denaturing conditions: Detergent can also be added to release membrane-associated polysomes from endoplasmic reticulum membranes; total polysomes are usually collected by centrifugation through, for example, a sucrose cushion.

In other embodiments, variations of the above-described general method are used to isolate membrane-associated polysomes from a total pool of polysomes. This allows for further enrichment of mRNA encoding secreted or transmembrane proteins. Various methods may be used to isolate membrane-associated polysomes from cultured cells and tissue, e.g., methods that employ differential centrifugation (Hall C, Lim L. Developmental changes in the composition of polyadenylated RNA isolated from free and membrane-bound polyribosomes of the rat forebrain, analyzed by translation in vitro. Biochem J. 1981 Apr. 15; 196(1):327-36), rate-zonal centrifugation (Rademacher and Steele, 1986, Isolation of undegraded free and membrane-bound polysomal mRNA from rat brain, J. Neurochem. 47(3):953-957), isopycnic centrifugation (Mechler, 1987, Isolation of messenger RNA from membrane-bound polysomes, Methods Enzymol. 152: 241-248), and differential extraction (Bommer et al., 1997, Isolation and characterization of eukaryotic polysomes, in Subcellular Fractionation, Graham and Rickwood (eds.), IRL Press, Oxford, pp. 280-285; incorporated herein by reference in its entirety) to isolate the membrane-associated polysomes (Heintz US publication 20050009028 incorporated in its entirety).
In other embodiments, affinity methods are used to isolate or purify tagged proteins using methods well known in the art including but not limited to including chromatography, solid phase chromatography precipitation, matrices, co-immunoprecipitation, etc.
In specific embodiments, molecularly tagged functional ribosomes are provided, bound to mRNA, that can bind a reagent for the molecular tag. In other embodiments, the molecularly tagged ribosomes are bound to a specific reagent or affinity reagent that is bound, covalently or non-covalently, to a solid surface, such as a bead, a resin, or a chromatography resin, e.g., agarose, sepharose, and the like. In other embodiments, other methods are used with or in place of affinity purification. In other embodiments, specific polysomes can be isolated utilizing optical sorting, fluorescence-based sorting or magnetic-based sorting methods and devices.
In certain embodiments, polysomes are not isolated from the post-mitochondrial supernatant or even from a cell or tissue lysate before being subject to affinity purification.
FIG. 2 a presents a schematic to illustrate affinity purification of eGFP-tagged polysomes using anti-GFP antibody-coated beads.
2) Isolation of mRNA from Ribosomes
Once the tagged ribosome has been isolated, the associated mRNA complexed with the protein may be isolated using chemical, mechanical or other methods well known in the art. For example, elution of mRNA is accomplished by addition of EDTA to buffers, which disrupts polysomes and allows isolation of bound mRNA for analysis (Schutz, et al. (1977), Nucl. Acids Res. 4:71-84; Kraus and Rosenberg (1982), Proc. Natl. Acad. Sci. USA 79:4015-4019). In addition, isolated polysomes (attached or detached from isolation matrix) can be directly inputted into RNA isolation procedures using reagents such as Tri-reagent (Sigma) or Triazol (Sigma). In particular embodiments, poly A.sup.+ mRNA is preferentially isolated by virtue of its hybridization of oligodT cellulose. Methods of mRNA isolation are described, for example, in Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y., both of which are hereby incorporated by reference in their entireties.
Nucleic Acid Sequences for Cloning, Expression and Regulation of Tagged Ribosomes
1) General
Methods that are well known to those skilled in the art can be used to construct vectors containing tagged ribosomal protein coding sequences operatively associated with the appropriate transcriptional and translational control signals required for expression. These methods include, for example, in vitro recombinant DNA techniques and in vivo genetic recombination. See, for example, the techniques described in Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.; and Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y.
2) Regulatory Sequences
Certain embodiments of the invention provide vectors, cell lines, and lines of organisms that contain nucleic acid constructs that comprise the coding sequence for a tagged ribosomal protein under the control of regulatory sequences. According to the methods described herein, the tagged ribosomal proteins are selectively expressed in a particular chosen cell type, cell subtype, molecular pathway, or circuit using regulatory elements naturally occurring or engineered to recapitulate a desired expression pattern. Such expression is achieved by driving the expression of the tagged ribosomal protein using regulatory sequences or a transcriptional unit from a characterizing or endogenously regulated gene expressed in the chosen cell type, subtype, pathway, circuit and/or point in development. In certain embodiments a particular cell type is one that resides within a mixed population of cells, comprising a discernable group of cells sharing a common characteristic. This group may be morphologically, anatomically, genetically, or functionally discernible. In other embodiments, this group may be morphologically, genetically, and developmentally indistinguishable, but be genetically distinguishable. Because of its selective expression, the population of cells may be characterized or recognized based on its positive expression of an endogenously regulated characterizing gene. Some or all of the regulatory sequences/elements may be incorporated into nucleic acids (including transgenes) to regulate the expression of tagged ribosomal protein coding sequences. Examples of regulatory sequences or elements include but are not limited to enhancer sequences, insulator sequences, silencer sequences, or a combination of said sequences. In certain embodiments, a gene that is not constitutively expressed, (i.e., exhibits some spatial or temporal restriction in its expression pattern) is used as a source of a regulatory sequence. In other embodiments, a gene that is constitutively expressed is used as a source of a regulatory sequence. In other embodiments a regulatory sequence is engineered or modified to achieve desired expression characteristics and patterns.
Regulatory regions can be whole or parts or the regulatory sequences from the loci of the genes of interest. For example, to drive specific expression of a tagged ribosomal protein (for example eGFP tagged L10a) in medium spiny neurons, regulatory sequences from a medium spiny neuron-specific gene locus, for example a Drd1a or Drd2 locus, can be utilized. In another example, to drive specific expression of a tagged ribosomal protein (for example eGFP tagged L10a) in motor neurons, regulatory sequences from a motor-specific gene locus, for example choline acetyl transferase (Chat) locus, can be utilized. Similarly, to drive specific expression of a tagged ribosomal protein (for example eGFP tagged L10a) in cerebellar cells, regulatory sequences from a cerebellar cell-specific gene loci. For example loci from Pcp2, Neurod1, Grm2, Grp, Septin4, or Aldh111 can be utilized, to drive specific expression in Purkinje cells, granule cells, golgi neurons, unipolar brush cells, Bergmann glial cells, and astrocytes, respectively. An entire locus, an entire non-coding region of a locus, a portion of a locus, or a modified locus can be utilized as the regulatory sequences to drive expression of tagged ribosomal proteins in a genetically targeted, cell subtype specific manner.
The tagged ribosomal gene coding sequences may placed under the transcriptional control of some or all of the regulatory sequences from a particular locus such that the tagged ribosomal gene is expressed in substantially the same expression pattern as the endogenously regulated gene found in that locus in the transformed organism, or at least in an anatomical region or tissue of the organism (by way of example, in the brain, spinal cord, heart, skin, bones, head, limbs, blood, muscle, peripheral nervous system, etc. of an animal) containing the population of cells to be marked by expression of the tagged ribosomal protein gene coding sequences. By “substantially the same expression pattern” is meant that the tagged ribosomal protein gene coding sequences are expressed in at least 50%, 60%, 70% 80%, 85%, 90%, 95%, and about 100% of the cells shown to express the endogenous characterizing gene by in situ hybridization, PCR, other gene expression detection methods or functional methods familiar to those with skill in the art.
The regulatory sequence is, e.g., a promoter or enhancer, or any other cis or trans activating element or transcriptional unit of a characterizing gene. In some embodiments this characterizing gene is endogenous to a host cell or host organism (or is an ortholog of an endogenous gene) and is expressed in a particular select population of cells of the organism. The regulatory sequence can be derived from a human, rat, or mouse or any mammalian gene associated with specific neuronal, cellular, and metabolic pathways. In certain embodiments, a non mammalian regulatory sequence can be utilized. Cis and trans, 5′ and 3′ regulatory sequences and transcriptional units from several known pathways are amenable for cell-type specific, cell subtype-specific, developmental stage-specific, temporal-specific, tissue-specific, pathway-specific, or circuit-specific expression of transgenes. Examples of known pathways include but are not limited to the adrenergic or noradrenergic neurotransmitter pathway, the dopaminergic neurotransmitter pathway, the GABAergic neurotransmitter pathway, the glutaminergic neurotransmitter pathway, the glycinergic neurotransmitter pathway, the histaminergic neurotransmitter pathway, a neuropeptidergic neurotransmitter pathway, a serotonergic neurotransmitter pathway, a nucleotide receptor-specific pathway, ion channels, transcriptions factors, markers of undifferentiated or not fully differentiated cells, the sonic hedgehog signaling pathway, calcium binding, or a neurotrophic factor receptor (tables in US2005/0009028, herein incorporated by reference).
In certain embodiments regulatory sequences that may be used to control expression can be derived from, include but are not limited to, the following animal transcriptional control regions that exhibit tissue specificity and that have been utilized in cell culture and transgenic animals Examples of transcriptional control regions are: elastase I gene control region, enolase promoter, insulin gene control region, immunoglobulin gene control region, mouse mammary tumor virus control region, albumin gene control region, alpha-fetoprotein gene control region, alpha 1-antitrypsin gene control region, beta.-globin gene control region, myelin basic protein gene control region, myosin light chain-2 gene control region, and gonadotropic releasing hormone gene control region. In other embodiments, the gene sequence from which the regulatory sequence derives can be protein kinase C, gamma, TH-elastin, Pax7, Eph receptors, islet-1 and Sonic hedgehog.
Nucleic acids to be used for regulating expression may include all or a portion of the upstream and/or downstream and 5′ and/or 3′ regulatory sequences from the naturally occurring or modified locus of a selected gene. The regulatory sequences preferably direct expression of the tagged ribosomal protein sequences in substantially the same pattern as the endogenous characterizing gene within transgenic organism, or tissue derived therefrom. This would provide of for any and all cis- and trans-acting regulatory sequences and transcriptional units that would direct expression of the tagged ribosomal protein in an endogenous regulatory fashion. A regulatory sequence can be 1 kb, 5 kb, 10 kb, 50 kb, 100 kb, 200 kb, 250 kb, 500 kb or even 1 Mb in length.
In certain embodiments, the nucleic acids encoding the molecularly tagged ribosomal proteins may be selectively expressed in random but distinct subsets of cells, as described in Feng et al. (2000, Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP, Neuron 28(1):41-51, which is hereby incorporated by reference in its entirety). Using such methods, independently generated transgenic lines may express the nucleic acids encoding the molecularly tagged ribosomal proteins in a unique pattern, perhaps pathway-specific, or circuit-specific, and although spatially separated, would all incorporate identical regulatory elements.
3) Placement and Construction of Regulatory Sequences and Transgenes
In certain embodiments the nucleotide sequences encoding the tagged ribosomal protein product may replace all or a portion of the characterizing gene coding sequences in a genomic clone/locus of the characterizing gene, leaving only all or part of the characterizing gene regulatory non-coding sequences.
In other embodiments, the tagged ribosomal gene coding sequences are inserted into or replace transcribed coding or non-coding sequences of the genomic characterizing gene sequences, for example, into or replacing a region of an exon or of the 3′ UTR of the characterizing gene genomic sequence.
In one embodiment, the tagged ribosomal protein gene coding sequence is inserted into or replaces a portion of the 3′ untranslated region (UTR) of the characterizing gene genomic sequence. In another embodiment, the coding sequence of the characterizing gene is mutated or disrupted to abolish characterizing gene expression from the nucleic acid construct without affecting the expression of the tagged fusion protein gene. In certain embodiments, the tagged ribosomal fusion protein gene coding sequence has its own internal ribosome entry site (IRES).
In some embodiments, the tagged ribosomal protein gene coding sequences are inserted using 5′ direct fusion wherein the tagged ribosomal protein gene coding sequences are inserted in-frame adjacent to the initial ATG sequence (or adjacent the nucleotide sequence encoding the first two, three, four, five, six, seven or eight amino acids of the characterizing gene protein product) of the characterizing gene, so that translation of the inserted sequence produces a fusion protein of the first methionine (or first few amino acids) derived from the characterizing gene sequence fused to the tagged ribosomal fusion protein gene protein.
In other embodiments, a tagged ribosomal fusion protein gene is inserted into a separate cistron in the 5′ region of the characterizing gene genomic sequence and has an independent IRES sequence. In certain embodiments, an IRES is operably linked to the tagged ribosomal fusion protein gene coding sequence to direct translation of the tagged fusion protein gene. The IRES permits the creation of polycistronic mRNAs from which several proteins can be synthesized under the control of an endogenous transcriptional regulatory sequence. Such a construct is advantageous because it allows marker proteins to be produced in the same cells that express the endogenous gene (Heintz, 2000, Hum. Mol. Genet. 9(6): 937-43; Heintz et al., WO 98/59060; Heintz et al., WO 01/05962; which are incorporated herein by reference in their entireties).
In certain embodiments, exogenous translational control signals, including, for example, the ATG initiation codon, can be provided by the characterizing gene or some other heterologous gene. The initiation codon is usually in phase with the reading frame of the desired coding sequence of the tagged ribosomal fusion protein gene to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression may be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see Bittner et al., 1987, Methods in Enzymol. 153: 516-44).
The construct can also comprise one or more selectable markers that enable identification and/or selection of recombinant vectors. The selectable marker may be the tagged ribosomal fusion protein gene product itself or an additional selectable marker not necessarily tied to the expression of the characterizing gene.
In certain embodiments where a tagged ribosomal fusion protein gene is expressed conditionally, the tagged ribosomal fusion protein gene coding sequence is embedded in the genomic sequence of the characterizing gene and is inactive unless acted on by a transactivator or recombinase, whereby expression of the tagged ribosomal fusion protein gene can then be driven by the characterizing gene regulatory sequences. In such a system, expression of the transactivator gene, as regulated by the characterizing gene regulatory sequences, activates the expression of the tagged fusion protein gene.
In a specific embodiment, a nucleic acid of the invention is expressed conditionally, using any type of inducible or repressible system available for conditional expression of genes known in the art, e.g., a system inducible or repressible by tetracycline (“tet system”); interferon; estrogen, ecdysone, or other steroid inducible system; Lac operator, progesterone antagonist RU486, or rapamycin (FK506). For example, a conditionally expressible nucleic acid of the invention can be created in which the coding region for the tagged ribosomal fusion protein gene (and, optionally also the characterizing gene) is operably linked to a genetic switch, such that expression of the tagged ribosomal fusion protein gene can be further regulated. One example of this type of switch is a tetracycline-based switch.
In several embodiments, the nucleic acids of the invention comprise all or a significant portion of the genomic sequence of the characterizing gene locus, at least all or a portion of the 5′ and/or 3′ regulatory sequences of the characterizing endogenously regulated gene, at least sufficient sequence 5′ and/or 3′ of the characterizing gene coding sequence to direct expression of the tagged ribosomal fusion protein gene coding sequences in the same expression pattern (temporal and/or spatial) as the endogenous counterpart of the characterizing gene. In certain embodiments, the nucleic acid of the invention comprises at least one exon, at least two exons, at least three exons, all but one exon, or all but two exons, of the characterizing endogenously regulated gene.
4) Expression Using a Binary System
Since the level of expression of the tagged ribosomal protein within a cell may needed in the efficiency of the isolation procedure, in certain embodiments of the invention, a binary system can be used, in which the endogenous regulatory sequence such as a promoter drives expression of a protein that then activates a second expression construct. This second expression construct uses a strong regulatory sequence such as a promoter to drive expression of the tagged ribosomal fusion protein at higher levels than is possible using the endogenous regulatory region itself.
In certain embodiments, a particular population-specific gene drives expression of a molecular switch (e.g., a recombinase, a transactivator) in a population-specific manner. This switch then activates high-level expression though a second regulatory element regulating expression of the tagged ribosomal protein.
For example, the molecularly tagged ribosomal protein coding sequence may be expressed conditionally, through the activity of a molecular switch gene which is an activator or suppressor of gene expression. In this case, the second gene encodes a transactivator, e.g., tetR, a recombinase, or FLP, whose expression is regulated by the characterizing gene regulatory sequences. The gene encoding the molecularly tagged ribosomal protein is linked to a conditional element, e.g., the tet promoter, or is flanked by recombinase sites, e.g., FRT sites, and may be located any where within the genome. In such a system, expression of the molecular switch gene, as regulated by the characterizing gene regulatory sequences, activates the expression of the molecularly tagged ribosomal protein.
5) Transcriptional Regulation Systems
In certain embodiments, the tagged ribosomal fusion protein gene can be expressed conditionally by operably linking at least the coding region for the tagged ribosomal fusion protein gene to all or a portion of the regulatory sequences from the characterizing gene's genomic locus, and then operably linking the tagged ribosomal fusion protein gene coding sequences and characterizing gene sequences to an inducible or repressible transcriptional regulation system. Transactivators in these inducible or repressible transcriptional regulation systems are designed to interact specifically with sequences engineered into the vector. Such systems include those regulated by tetracycline (“tet systems”), interferon, estrogen, ecdysone, Lac operator, progesterone antagonist RU486, and rapamycin (FK506) with tet systems being particularly preferred (see, e.g., Gingrich and Roder, 1998, Annu. Rev. Neurosci. 21: 377-405; incorporated herein by reference in its entirety). These drugs or hormones (or their analogs) act on modular transactivators composed of natural or mutant ligand binding domains and intrinsic or extrinsic DNA binding and transcriptional activation domains In certain embodiments, expression of the detectable or selectable marker can be regulated by varying the concentration of the drug or hormone in medium in vitro or in the diet of the transformed organism in vivo.
The inducible or repressible genetic system can restrict the expression of the detectable or selectable marker either temporally, spatially, or both temporally and spatially.
In other embodiments, expression of the tagged ribosomal fusion protein gene is regulated by using a recombinase system that is used to turn on or off tagged ribosomal fusion protein gene expression by recombination in the appropriate region of the genome in which the marker gene is inserted. Such a recombinase system, in which a gene that encodes a recombinase can be used to turn on or off expression of the tagged ribosomal fusion protein gene (for review of temporal genetic switches and “tissue scissors” using recombinases, see Hennighausen and Furth, 1999, Nature Biotechnol. 17: 1062-63). Exclusive recombination in a selected cell type may be mediated by use of a site-specific recombinase such as Cre, FLP-wild type (wt), FLP-L or FLPe. Recombination may be effected by any art-known method, e.g., the method of Doetschman et al. (1987, Nature 330: 576-78; incorporated herein by reference in its entirety); the method of Thomas et al., (1986, Cell 44: 419-28; incorporated herein by reference in its entirety); the Cre-loxP recombination system (Stemberg and Hamilton, 1981, J. Mol. Biol. 150: 467-86; Lakso et al., 1992, Proc. Natl. Acad. Sci. USA 89: 6232-36; which are incorporated herein by reference in their entireties); the FLP recombinase system of Saccharomyces cerevisiae (O'Gorman et al., 1991, Science 251: 1351-55); the Cre-loxP-tetracycline control switch (Gossen and Bujard, 1992, Proc. Natl. Acad. Sci. USA 89: 5547-51); and ligand-regulated recombinase system (Kellendonk et al., 1999, J. Mol. Biol. 285: 175-82; incorporated herein by reference in its entirety). Preferably, the recombinase is highly active, e.g., the Cre-loxP or the FLPe system, and has enhanced thermostability (Rodriguez et al, 2000, Nature Genetics 25: 139-40; incorporated herein by reference in its entirety).
In certain embodiments, a recombinase system can be linked to a second inducible or repressible transcriptional regulation system. For example, a cell-specific Cre-loxP mediated recombination system (Gossen and Bujard, 1992, Proc. Natl. Acad. Sci. USA 89: 5547-51) can be linked to a cell-specific tetracycline-dependent time switch detailed above (Ewald et al., 1996, Science 273: 1384-1386; Furth et al. Proc. Natl. Acad. Sci. U.S.A. 91: 9302-06 (1994); St-Onge et al, 1996, Nucleic Acids Research 24(19): 3875-77; which are incorporated herein by reference in their entireties).
In one embodiment, an altered cre gene with enhanced expression in mammalian cells is used (Gorski and Jones, 1999, Nucleic Acids Research 27(9): 2059-61; incorporated herein by reference in its entirety).
In a specific embodiment, the ligand-regulated recombinase system of Kellendonk et al. (1999, J. Mol. Biol. 285: 175-82; incorporated herein by reference in its entirety) can be used. In this system, the ligand-binding domain (LBD) of a receptor, e.g., the progesterone or estrogen receptor, is fused to the Cre recombinase to increase specificity of the recombinase.
6) Sources of Nucleic Acids
Nucleic acids comprising the characterizing gene sequences and tagged ribosomal fusion protein gene coding sequences can be obtained from any available source. In most cases, all or a portion of the characterizing gene sequences and/or the tagged ribosomal fusion protein gene coding sequences are known, for example, in publicly available databases such as GenBank, UniGene and the Mouse Genome Informatic (MGI) Database to name just a few, or in private subscription databases. With a portion of the sequence in hand, hybridization probes can be designed using highly routine methods in the art (for example filter hybridization or PCR amplification) to identify clones containing the appropriate sequences for example in a library or other source of nucleic acid. If the sequence of the gene of interest from one species is known and the counterpart gene from another species is desired, it is routine in the art to design probes based upon the known sequence. The probes hybridize to nucleic acids from the species from which the sequence is desired, for example, hybridization to nucleic acids from genomic or DNA libraries from the species of interest. By way of example and not limitation, genomic clones can be identified by probing a genomic DNA library under appropriate hybridization conditions, e.g., high stringency conditions, low stringency conditions or moderate stringency conditions, depending on the relatedness of the probe to the genomic DNA being probed.
7) Vectors
Briefly, the characterizing gene genomic sequences are preferably in a vector that can accommodate lengths of sequence (for example, 10 kb's of sequence), such as cosmids, yeast artificial chromosomes (YACs) and bacterial artificial chromosomes (BACs), and encompass at least 50, 70, 80, 100, 120, 150, 200, 250, 300, 400, 500 or 1000 kb of sequence that comprises all or a portion of the characterizing gene sequence. The larger the vector insert, the more likely it is to identify a vector that contains the characterizing gene cis and trans sequences of interest. In some embodiments the vector containing the entire genomic sequence (i.e. genomic locus) for the characterizing endogenously regulated gene is present. In other embodiments the entire genomic sequence cannot be accommodated by a single vector or such a clone is not available. In these instances (or when it is not known whether the clone contains the entire genomic sequence), the vector contains at least the characterizing gene sequence with the start, i.e., the most 5′ end, of the coding sequence in the approximate middle of the vector insert containing the genomic sequences and/or has at least 20 kb, 30 kb, 40 kb, 50 kb, 60 kb, 80 kb, 100 kb or 200 kb of genomic sequence on either side of the start of the characterizing gene coding sequence. This can be determined by any method known in the art, for example, but not by way of limitation, by sequencing, restriction mapping, PCR amplification assays, etc.
Once the appropriate vector containing the regulatory region/regulatory gene sequences has been created, the tagged ribosomal fusion protein gene can be incorporated into the characterizing gene sequence by any method known in the art for manipulating DNA. In one embodiment, homologous recombination in bacteria is used for target-directed insertion of the tagged ribosomal fusion protein gene sequence into the genomic DNA encoding the characterizing endogenously regulated gene and sufficient regulatory sequences to promote expression of the gene in its endogenous expression pattern, which characterizing gene sequences have been inserted into a vector. The vector comprising the tagged ribosomal fusion protein gene and regulatory gene sequences is then introduced into the genome of a potential founder organism for generating a line of transformed organisms, using methods well known in the art, e.g., those methods described herein. Such transformed organisms are then screened for expression of the tagged ribosomal fusion protein gene coding sequences that mimics the expression of the endogenous characterizing gene. Several different constructs containing nucleic acids of the invention may be introduced into several potential founder organisms and the resulting transformed organisms are then screened for the best, (e.g., highest level) and most accurate (best mimicking expression of the endogenous characterizing gene) expression of the tagged ribosomal fusion protein gene coding sequences.
The nucleic acid construct can be used to transform a host or recipient cell or organism using well known methods, e.g., those described herein. Transformation can be either a permanent or transient genetic change. In one aspect of the invention, a vector is used for stable integration of the nucleic acid construct into the genome of the cell. In another aspect, the vector is maintained episomally.
8) Bacterial Artificial Chromosomes (BACs) and Other Vectors
The methods described herein provide transformed organisms, e.g., transgenic mice that express a tagged ribosomal protein within a chosen cell type. In some embodiments, BAC-mediated recombination (Yang, et al., 1997, Nat. Biotechnol. 15(9):859-865) is used to create the transformed organism. Such expression is achieved by using the endogenous regulatory sequences and transcriptional units of a particular gene, wherein the expression of gene is a defining characteristic of the chosen cell type (as also described in PCT/US02/04765, entitled “Collections of Transgenic Animal Lines (Living Library)” by Serafini, published as WO 02/064749 on Aug. 22, 2002, which is incorporated by reference herein in its entirety). A collection of transgenic animals expressing tagged ribosomal proteins within a set of chosen cell types is assembled, and this is often referred to as a BACarray collection.
In another embodiment, a nucleic acid of the invention is inserted into a yeast artificial chromosome (YAC) (Burke et al., 1987 Science 236: 806-12; and Peterson et al., 1997, Trends Genet. 13: 61). A collection of transgenic animals expressing tagged ribosomal proteins within a set of chosen cell types or subtypes is assembled, and this is often referred to as a YACarray collection.
In other embodiments, the nucleic acid of the invention is inserted into another vector developed for the cloning of large segments of mammalian DNA, such as a cosmid or bacteriophage P1 (Sternberg et al., 1990, Proc. Natl. Acad. Sci. USA 87: 103-07). The approximate insert size is about 30-35 kb for cosmids and 100 kb for bacteriophage P1.
In another embodiment, the nucleic acid of the invention is inserted into a P-1 derived artificial chromosome (PAC) (Mejia et al., 1997, Retrofitting vectors for Escherichia coli -based artificial chromosomes (PACs and BACs) with markers for transfection studies, Genome Res. 7(2):179-86). The maximum insert size is about 300 kb.
BACs and other vectors used in the methods described here often can accommodate, and in certain embodiments comprise, large pieces of heterologous DNA such as genomic sequences. Such vectors can contain an entire genomic locus, or at least sufficient sequences to confer endogenous regulatory expression pattern and to insulate the expression of coding sequences from the effect of regulatory sequences surrounding the site of integration of the nucleic acid in the genome to mimic better wild type expression. When entire genomic loci or portions thereof are used, few, if any, site-specific expression problems are encountered, unlike insertions of nucleic acids into smaller sequences. When insertions of nucleic acids are too large, other problems can be encountered. In some embodiments, the vector is a BAC containing genomic sequences into which a selected sequence encoding a molecular tag that has been inserted by directed homologous recombination in bacteria, e.g., by the methods of Heintz WO 98/59060; Heintz et al., WO 01/05962; Yang et al., 1997, Nature Biotechnol. 15: 859-865; Yang et al., 1999, Nature Genetics 22: 327-35; which are incorporated herein by reference in their entireties.
Using such methods, a BAC can be modified directly in a recombination-deficient E. coli host strain by homologous recombination. i.e., a cell that cannot independently support homologous recombination, e.g., Rec A.sup.-. In one embodiment, homologous recombination in bacteria is used for target-directed insertion of a sequence encoding a molecularly tagged ribosomal protein into the genomic DNA encoding sufficient regulatory sequences (termed “characterizing gene sequences”) to promote expression of the tagged ribosomal protein in the endogenous expression pattern of the characterizing gene, which sequences have been inserted into the BAC. The BAC comprising the tagged fusion protein sequence under the control/regulation of a regulatory region comprising sequences from a genomic locus of the desired characterizing gene is then recovered and introduced into the genome of a potential founder organism for a line of transformed organisms. In one aspect, the particular nucleotide sequence that has been selected to undergo homologous recombination is contained in an independent origin based cloning vector introduced into or contained within the host cell, and neither the independent origin based cloning vector alone, nor the independent origin based cloning vector in combination with the host cell, can independently support homologous recombination (e.g., is RecA). The exact method used to introduce the tagged ribosomal protein encoding sequence and to remove (or not) the RecA (or other appropriate recombination enzyme) will depend upon the nature of the BAC library used (for example, the selectable markers present on the BAC vectors) and such modifications are within the skill in the art.
Once the BAC containing the characterizing gene regulatory sequences and molecularly tagged ribosomal protein coding sequences in the desired configuration is identified, it can be isolated from the host E. coli cells using routine methods and used to make transformed organisms as described within.
Alternatively, the BAC can also be engineered or modified by “E-T cloning,” as described by Muyrers et al (1999, Nucleic Acids Res. 27(6): 1555-57, incorporated herein by reference in its entirety). Using these methods, specific DNA may be engineered into a BAC independently of the presence of suitable restriction sites. This method is based on homologous recombination mediated by the recE and recT proteins (“ET-cloning”) (Zhang et al., 1998, Nat. Genet. 20(2): 123-28; incorporated herein by reference in its entirety).
Host Cells and Organisms Engineered to Express Molecularly Tagged Ribosomes
1) Introduction of Vectors into Host Cells
In one aspect, a vector containing the nucleic acid encoding the regulatory sequences and the molecularly tagged fusion protein can be introduced transiently or stably into the genome of a host cell. In another aspect, the vector can be transiently transfected wherein it is not integrated, but is maintained as an episome. The terms “host cell” and “recombinant host cell” are used interchangeably herein.
It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.
A host cell can be any prokaryotic (e.g., bacterium such as E. coli ) or eukaryotic cell (e.g., a cell from a yeast, plant, insect (e.g., Drosophila ), amphibian, amniote, or mammal, to name but a few. In certain embodiments the host cell is a human cell, a rodent cell, a mouse cell, a rat cell, a mammalian cell, an immortalized cultured cell or primary human or rodent cell. In specific embodiments, the host cells are human or rodent embryonic stem cells, neuronal stem cells, hippocampal stem cells, hippocampal progenitor cells, or partially differentiated pluripotent cells, or tumor cells or cancer cells (particularly circulating cancer cells such as those resulting from leukemias and other blood system cancers). Methods can utilize genetically engineered host cells that contain any of the foregoing tagged ribosomal protein coding sequences, optionally operatively associated with a regulatory element (preferably from a characterizing gene, as described above) that directs the expression of the coding sequences in the host cell. Both cDNA and genomic sequences can be cloned and expressed.
In one aspect, the host cell is recombination deficient, i.e., Rec.sup.-, and used for BAC recombination. In specific embodiments the host cell may contain more than one type of ribosomal fusion, where the fusion of the different ribosomal is to the same or different tags. Alternatively, a single ribosomal may be fused to more than one tag, for example, at both is N-terminal and C-terminal ends.
A vector containing a nucleotide sequence can be introduced into the desired host cell by methods known in the art, e.g., transfection, transformation, transduction, electroporation, infection, microinjection, cell fusion, DEAE dextran, calcium phosphate precipitation, liposomes, LIPOFECTIN.™., lysosome fusion, synthetic cationic lipids, use of a gene gun or a DNA vector transporter, such that the nucleotide sequence is transmitted to offspring in the line. For various techniques for transformation or transfection of mammalian cells, see Keown et al., 1990, Methods Enzymol. 185: 527-37; Sambrook et al., 2001, Molecular Cloning, A Laboratory Manual, Third Edition, Cold Spring Harbor Laboratory Press, N.Y.
In certain embodiments, the vector is introduced into a cultured cell. In other embodiments, the vector is introduced into a proliferating cell (or population of cells), e.g., a tumor cell, a stem cell, a blood cell, a bone marrow cell, a cell derived from a tissue biopsy, etc.
2) Introduction of Vectors to Create Transgenic Animals
Particular embodiments encompass methods of introduction of the vector containing the nucleic acid, using pronuclear microinjection into the mononucleus of a mouse embryo or infection with a viral vector comprising the construct. Methods of pronuclear injection into mouse embryos are well-known in the art and described in Hogan et al. 1986, Manipulating the Mouse Embryo, Cold Spring Harbor Laboratory Press, New York, N.Y. and Wagner et al., U.S. Pat. No. 4,873,191, issued Oct. 10, 1989, herein incorporated by reference in their entireties. Viral methods of inserting nucleic acids are known in the art. Targeted delivery can utilize lentivirus, retrovirus, adenovirus, herpes simplex virus, adeno associated virus, or any other virus amenable to gene delivery.
3) Transgenic Organisms
Any model organism can be engineered to express a molecularly tagged ribosome using methods well known in the art. These can include but are not limited to non-human primates, mice, rats, sheep, dogs, cows, goats, chickens, amphibians, fish, zebrafish, ( Danio rerio ), flies ( Drosophila melanogaster ), worms ( Caenorhabditis elegans ), or yeast (or other fungi). A transgenic animal is a non-human animal in which one or more of the cells of the animal includes a nucleic acid that is non-endogenous (i.e., heterologous) and is present as an extrachromosomal element in a portion of its cell or stably integrated into its germ line DNA (i.e., in the genomic sequence of most or all of its cells). In certain embodiments a transgenic animal comprises stable changes to the germline sequence. Heterologous nucleic acid is introduced into the germ line of such a transgenic animal by genetic manipulation of, for example, embryos or embryonic stem cells of the host animal
The heterologous nucleic acid may integrate into the genome of the founder organism by random integration. If random, the integration preferably does not knock out, e.g., insert into, an endogenous gene(s) such that the endogenous gene is not expressed or is mis-expressed.
In other embodiments, the nucleic acid of the may integrate by a directed method, e.g., by directed homologous recombination (“knock-in”), Chappel, U.S. Pat. No. 5,272,071; and PCT publication No. WO 91/06667, published May 16, 1991; U.S. Pat. No. 5,464,764; Capecchi et al., issued Nov. 7, 1995; U.S. Pat. No. 5,627,059, Capecchi et al issued, May 6, 1997; U.S. Pat. No. 5,487,992, Capecchi et al., issued Jan. 30, 1996).
Transformed organisms, e.g., transgenic animals, can be generated by random integration of a vector into the genome of the organism, for example, by pronuclear injection in an animal zygote as described above. Other methods involve introducing the vector into cultured embryonic cells, for example ES cells using methods of injection or electroporation well known in the art, and then introducing the transformed cells into animal blastocysts, thereby generating a “chimeras” or “chimeric animals”, in which only a subset of cells have the altered genome. Chimeras are often used for breeding purposes in order to generate the desired transgenic animal Animals having a heterozygous alteration are generated by breeding of chimeras. Male and female heterozygotes are typically bred to generate homozygous animals
Methods for generating transgenic animals via embryo manipulation and microinjection, particularly animals such as mice, have become conventional in the art and are described, for example, in U.S. Pat. Nos. 4,736,866 and 4,870,009, U.S. Pat. No. 4,873,191, in Hogan, Manipulating the Mouse Embryo, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986) and in Wakayama et al., 1999, Proc. Natl. Acad. Sci. USA, 96:14984-89. Similar methods are used for production of other transgenic animals
A transgenic founder animal can be identified based upon the presence of the nucleic acid introduced in its genome and/or expression of mRNA in tissues or cells of the animals A transgenic founder animal can then be used to breed additional animals carrying the nucleic acid of. These transgenic animals can further be bred to other transgenic animals carrying other heterologous nucleic acids. Progeny harboring homologously recombined or integrated DNA in their germline cells can be used to breed animals in which all cells of the animal contain the homologously recombined DNA by germline transmission of the nucleic acid of interest.
Clones of the non-human transgenic animals described herein can also be produced according to the methods described in Wilmut et al., 1997, Nature 385: 810-13 and PCT Publication NOS. WO 97/07668 and WO 97/07669.
Once the transgenic mice are generated they may be bred and maintained using methods well known in the art. By way of example, the mice may be housed in an environmentally controlled facility maintained on a 10 hour dark: 14 hour light cycle. Mice are mated when they are sexually mature (6 to 8 weeks old). In certain embodiments, the transgenic founders or chimeras are mated to an unmodified animal In one embodiment, the transgenic founder or chimera is mated to C57BL/6 mice (Jackson Laboratories). In a specific embodiment where the nucleic acid is introduced into ES cells and a chimeric mouse is generated, the chimera is mated to 129/Sv mice, which have the same genotype as the embryonic stem cells. Protocols for successful creation and breeding of transgenic mice are known in the art (Manipulating the Mouse Embryo. A Laboratory Manual, 2nd edition. B. Hogan, Beddington, R., Costantini, F. and Lacy, E., eds. 1994. Cold Spring Harbor Laboratory Press: Plainview, N.Y.).
Methods to establish heterozygousity or homozygousity are well known in the art and utilize PCR and Southern blotting.
In some embodiments, the transgenic mice are so highly inbred to be genetically identical except for sexual differences. The homozygotes are tested using backcross and intercross analysis to ensure homozygosity. Homozygous lines for each integration site in founders with multiple integrations are also established. Brother/sister matings for 20 or more generations define an inbred strain. In another preferred embodiment, the transgenic lines are maintained as hemizygotes.
In an alternative embodiment, individual genetically altered mouse strains are also cryopreserved rather than propagated. Methods for freezing embryos for maintenance of founder animals and transgenic lines are known in the art. Methods for reconstituting frozen embryos and bringing the embryos to term are known in the art.
The nucleic acid encoding the molecularly tagged ribosomal protein may be introduced into the genome of a founder plant (or embryo that gives rise to the founder plant) using methods well known in the art (Newell, 2000, Plant transformation technology. Developments and applications, Mol. Biotechnol. 16(1):53-65; Kumar and Fladung, 2001, Controlling transgene integration in plants, Trends in Plant Science 6 (4): 155-159). The nucleic acid encoding the molecularly tagged ribosomal protein may be introduced into the genome of bacteria and yeast using methods described in Ausubel et al., 1989, Current Protocols in Molecular Biology, Green Publishing Associates and Wiley Interscience, N.Y., Chapters 1 and 13, respectively).
4) Screening for Expression of Tagged Ribosomal Proteins in Transgenic Organisms
Potential founder organisms for a line of transformed organisms can be screened for expression of the molecularly tagged fusion protein coding sequence by ribosomes in the population of cells characterized by expression of the endogenous characterizing gene.
In one embodiment, immunohistochemistry using an antibody specific for the molecular tag or a marker activated or repressed thereby is used to detect expression of the molecular tag. In another embodiment, other methods and devices are used to detect the tag, such as with the use of optics-based sorting or magnetics-based sorting.
Transformed organisms that exhibit appropriate expression (e.g., detectable expression having substantially the same expression pattern as the endogenous characterizing gene in a corresponding non-transgenic organism or anatomical region thereof, i.e., detectable expression in at least 50%, 60%, 70%, 80%, 90% or, preferably, 95% of the cells shown to express the endogenous gene by in situ hybridization) are selected as lines of transformed organisms.
BACarray Lines
In some embodiments, a collection of lines of transformed organisms that contain a selected subset of cells or a cell population expressing molecularly-tagged ribosomes is provided. In some aspects, when the transformed organism has been created utilizing BAC-related methods, this is referred to as a BACarray collection. The collection comprises at least 2 individual lines, preferably at least 5, 10, 25, 50, 100, 500, 1000, 1500, 2000, 2500, 5000, 10,000, 15,000, or 30,000 individual lines. Each individual line is selected for the collection based on the identity of the subset of cells in which the molecularly tagged ribosomes are expressed.
In one embodiment, a BACarray line related to neuronal diseases (neurodegenerative, neuropsychiatric, behavioral, etc) can be created. These include, but are not limited to BACarray lines specific for expression for cells involved in disease processes such as depression, obesity, anxiety, epilepsy, sleep, Parkinson's disease, ADHD, Huntington's disease, addiction, dementia, Alzheimer's disease, ALS, and the like. For example, see FIG. 1 for a representative BACarray collection specific to central nervous system diseases.
In one embodiment, a BACarray collection relating to sub-regions of a tissue, for example regions of the central nervous system can be created. These include, but are not limited to BACarray lines specific for expression in only striatal cells (for example expression in medium spiny neurons, striatonigral and/or striatopallidal neurons), in only cerebellar cells (for example expression in Purkinje neurons, astrocytes, Bergmann glia, granule cells, etc), in only hippocampal cells (for example, expression in CA1 neurons, CA2 neurons, CA3/4 neurons, glia, astrocytes, DG neurons), in only hypothalamic cells, in only spinal cord cells, in only pineal gland cells, and the like.
In another related embodiment, a BACarray collection relating to a particular biological function can be created. These include but are not limited to BACarray lines specific for functions related to myelination, excitatory neural transmission, motor function, migration, adhesion, infiltration, processing of noxious stimuli, processing of sensory stimuli, visual processing, auditory processing, olfactory processing, vestibular control, regulation of feeding and satiety, regulation of wakefulness and sleep, regulation of reward behavior (for example as how it relates to addictive behavior), and the like.
Analyses of mRNA Species
The embodiments described herein provide for translation profiling and molecular phenotyping of particular cells and/or tissues. mRNA complexed in polysomes with tagged ribosomal proteins are isolated and can be analyzed by any method known in the art. In one aspect, the translational profile of cells expressing the tagged ribosomal proteins can be analyzed by isolating the mRNA and constructing cDNA libraries or by labeling the RNA for gene expression analysis, for example by disposing the mRNA on a microarray. Embodiments of this invention utilize techniques described in US 2005/0009028, which is herein incorporated in its entirety.
In one aspect, mRNA bound by the tagged ribosomal proteins may be used to produce a cDNA library and, in fact, a collection of such cell type-specific, cell-subtype specific, tissue-specific, organism-specific, disease-specific, function-specific, cDNA libraries may be generated from different populations of isolated cells. Such cDNA libraries are useful to analyze gene expression, isolate and identify cell type-specific genes, splice variants and non-coding RNAs, as well as identify co-regulated gene sets for a particular cell related to a function or a disease state. In another aspect, such cell-type specific libraries prepared from mRNA bound by, and isolated from, the tagged ribosomal proteins from treated and untreated or transgenic or otherwise manipulated cells/animals having can be used, for example in subtractive hybridization procedures, to identify genes expressed at higher or lower levels in response to a particular treatment or in a disease state as compared to untreated animals The mRNA isolated from the tagged ribosomal proteins may also be analyzed using particular microarrays generated and analyzed by methods well known in the art. Gene expression analysis using microarray technology is well known in the art. Methods for making microarrays are taught, for example, in U.S. Pat. No. 5,700,637 by Southern, U.S. Pat. No. 5,510,270 by Fodor et al. and PCT publication WO 99/35293 by Albrecht et al., which are incorporated by reference in their entireties. By probing a microarray with various populations of mRNAs, transcribed genes in certain cell populations can be identified. Moreover, the pattern of gene expression in different cell types of cell states may be readily compared.
The mRNA bound by the tagged ribosomal proteins may be analyzed, for example by northern blot analysis, PCR, RNase protection, etc., for the presence of mRNAs encoding certain protein products and for changes in the presence or levels of these mRNAs depending on manipulation.
In yet another embodiment, specific cells or cell populations that express a potential a molecularly tagged ribosomal protein are isolated from the collection and analyzed for specific protein-protein interactions or an entire protein profile using proteomics methods known in the art, for example, chromatography, mass spectroscopy, 2D gel analysis, etc.
Other types of assays may be used to analyze the cell population expressing the molecularly tagged ribosomal protein either in vivo, in explanted or sectioned tissue or in the isolated cells, for example, to monitor the response of the cells to a certain manipulation/treatment or candidate agent (for example, a small molecule, an antibody, a hybrid antibody, an antibody fragment, a siRNA, an antisense RNA, an aptamer, a protein, or a peptide) or to compare the response of the animals, tissue or cells to expression of the target or inhibitor thereof, with animals, tissue or cells from animals not expressing the target or inhibitor thereof. The cells may be monitored, for example, but not by way of limitation, for changes in electrophysiology, physiology (for example, changes in physiological parameters of cells, such as intracellular or extracellular calcium or other ion concentration, change in pH, change in the presence or amount of second messengers, cell morphology, cell viability, indicators of apoptosis, secretion of secreted factors, cell replication, contact inhibition, etc.), morphology, etc.
In particular embodiments, the isolated mRNA is used to probe a comprehensive expression library (see, e.g., Serafini et al., U.S. Pat. No. 6,110,711, issued Aug. 29, 2000, which is incorporated by reference herein). The library may be normalized and presented in a high density array, such as a microarray.
In a particular embodiment, a subpopulation of cells expressing a molecularly tagged ribosomal protein is identified and/or gene expression analyzed using the methods of Serafini et al., WO 99/29877 entitled “Methods for defining cell types,” which is hereby incorporated by reference in its entirety.
Data from such analyses may be used to generate a database of gene expression analysis for different populations of cells in the animal or in particular tissues or anatomical regions, for example, in the brain. Using such a database together with bioinformatics tools, such as hierarchical and non-hierarchical clustering analysis and principal components analysis, cells are “fingerprinted” for particular indications from healthy and disease-model animals or tissues, co-regulated gene sets for a particular function, and the like.
Applications and Considerations
1) Sensitivity
The methods provided herein are applicable to any cell, tissue, or organism as well as any disease or disorder. The sensitivity afforded by the ability to interrogate an individual cell subtype in a complex heterogeneous tissue or organ system provides a resolution than can be greater than with other techniques such as whole-tissue microarrays and in situ hybridization In some embodiments the TRAP method of detecting one or more cell subtype-specific mRNAs/genes allows for identification of greater than 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% and even 50% of the mRNAs/genes enriched in that cell type which are not detectable by other means such as whole-tissue microarrays or in situ hybridization
2) Diseases and Disorders
The methods provided herein are applicable to any cell, tissue, or organism as well as any disease or disorder. Several disease are cited in, but not limited to those found in the ‘The Merck Manual of Diagnosis and Therapy’, often called simply ‘The Merck Manual’ (2006). The diseases and disorders can be central nervous system disorders, peripheral nervous system disorders, and non nervous system disorders.
Examples of neurodegenerative diseases/disorders include, but are not limited to: alcoholism, Alexander's disease, Alper's disease, Alzheimer's disease, Amyotrophic lateral sclerosis, Ataxia telangiectasia, Batten disease (also known as Spielmeyer-Vogt-Sjogren-Batten disease), Bovine spongiform encephalopathy (B SE), Canavan disease, Cockayne syndrome, Corticobasal degeneration, Creutzfeldt-Jakob disease, Huntington's disease, HIV-associated dementia, Kennedy's disease, Krabbe's disease, Lewy body dementia, Machado-Joseph disease (Spinocerebellar ataxia type 3), Multiple sclerosis, Multiple System Atrophy, Narcolepsy, Neuroborreliosis, Parkinson's disease, Pelizaeus-Merzbacher Disease, Pick's disease, Primary lateral sclerosis, Prion diseases, Refsum's disease, Sandhoffs disease, Schilder's disease, Subacute combined degeneration of spinal cord secondary to Pernicious Anaemia, Schizophrenia, Spielmeyer-Vogt-Sjogren-Batten disease (also known as Batten disease), Spinocerebellar ataxia (multiple types with varying characteristics), Spinal muscular atrophy, Steele-Richardson-Olszewski disease, and Tables dorsalis.
Examples of neuropsychiatric diseases/disorders include, but are not limited to: depression, bipolar disorder, mania, obsessive compulsive disease, addiction, ADHD, schizophrenia, auditory hallucinations, eating disorders, hysteria, autism spectrum disorders and personality disorders.
Examples of neurodevelopmental diseases/disorders include, but are not limited to: attention deficit hyperactivity disorder (ADHD), attention deficit disorder (ADD), schizophrenia, obsessive-compulsive disorder (OCD), mental retardation, autistic spectrum disorders (ASD), cerebral palsy, Fragile-X Syndrome, Downs Syndrome, Rett's Syndrome, Asperger's syndrome, Williams-Beuren Syndrome, childhood disintegrative disorder, articulation disorder, learning disabilities (i.e., reading or arithmetic), dyslexia, expressive language disorder and mixed receptive-expressive language disorder, verbal or performance aptitude. Diseases that can result from aberrant neurodevelopmental processes can also include, but are not limited to bi-polar disorders, anorexia, general depression, seizures, obsessive compulsive disorder (OCD), anxiety, bruixism, Angleman's syndrome, aggression, explosive outburst, self injury, post traumatic stress, conduct disorders, Tourette's disorder, stereotypic movement disorder, mood disorder, sleep apnea, restless legs syndrome, dysomnias, paranoid personality disorder, schizoid personality disorder, schizotypal personality disorder, antisocial personality disorder, borderline personality disorder, histrionic personality disorder, narcissistic personality disorder, avoidant personality disorder, dependent personality disorder, reactive attachment disorder; separation anxiety disorder; oppositional defiant disorder; dyspareunia, pyromania, kleptomania, trichotillomania, gambling, pica, neurotic disorders, alcohol-related disorders, amphetamine-related disorders, cocaine-related disorders, marijuana abuse, opioid-related disorders, phencyclidine abuse, tobacco use disorder, bulimia nervosa, delusional disorder, sexual disorders, phobias, somatization disorder, enuresis, encopresis, disorder of written expression, expressive language disorder, mental retardation, mathematics disorder, transient tic disorder, stuttering, selective mutism, Crohn's disease, ulcerative colitis, bacterial overgrowth syndrome, carbohydrate intolerance, celiac sprue, infection and infestation, intestinal lymphangiectasia, short bowel syndrome, tropical sprue, Whipple's disease, Alzheimer's disease, Parkinson's Disease, ALS, spinal muscular atrophies, and Huntington's Disease. Further examples, discussion, and information on neurodevelopmental disorders can be found, for example, through the Neurodevelopmental Disorders Branch of the National Institute of Mental Health (worldwide website address at nihm.nih.gov/dptr/b2-nd.cfm).
3) Profiling of mRNA Species
In one embodiment, the invention provides for a method to obtain a translational profile of a cell type of interest. The method comprises expressing a tagged ribosomal protein under the control of a regulatory sequence specific to a gene expressed in the cell type of interest, isolating mRNAs complexed with the ribosomal protein from the cell, and identifying the mRNAs, thereby obtaining a translational profile for the cell type of interest.
In one exemplary embodiment the cell types of interest are striatonigral and striatopallidal cells. L10a fused to eGFP under the control of a Drd1a or a Drd2 specific regulatory sequence is expressed and translational profiles are obtained. Tables 10, 13, 17, and 18 identify genes that are translationally profiled in striatonigral and striatopallidal cells.
In another exemplary embodiment the cell type of interest are cholinergic motor neurons. Ribosomal protein L10a fused to eGFP under the control of a choline acetyl transferase (chat)-specific regulatory sequence is expressed and translational profiles are obtained. Table 19 identifies genes that are translationally profiled in cholinergic motor neurons. In this embodiment, the cholinergic motor neurons are spatially separated.
In another exemplary embodiment the cell type of interest are cerebellar neurons and glia, specifically Purkinje neurons and Bergmann glia. Ribosomal protein L10a fused to eGFP under the control of either a Pcp2 or Septin4-specific regulatory sequence is expressed and translational profiles are obtained. Table 20 identifies genes that are translationally profiled in cerebellar Purkinje neurons and Bergmann glia.
In another embodiment a gene translational profile following the manipulation of a cell, tissue or organism is obtained. The method comprises manipulating a cell, tissue or organism, using the TRAP methodology to obtain a translational profile, and comparing the profiled to a reference profile from an non-manipulated cell, tissue or organism. Manipulations include but are not limited to:
a. Pharmacological: for example administration of a candidate agent such as a small molecule antagonist or an agonist; administration of a pharmacological agent to recapitulate a disease such as the administration of MPTP or OHDA to an animal model or cell culture to induce a Parkinson's disease-like state; or administration of a drug or substance of abuse, for example cocaine or alcohol; b. Genetic: for example introduction of a germline or non-germline mutation or transgene to recapitulate an animal or cellular model of a disease or disorder such as ataxia, Parkinson's disease, Alzheimer's disease, autism spectrum disorders and the like; c. Mechanical: for example surgical treatment; and/or d. Environmental: for example change of habitat, climatic change, reversal of day-nite cycles; for example induction of a chronic-mild-stress protocol, art-recognized, to recapitulate a depression-like phenotype perturbations. Examples of candidate agents are but not limited to a small molecule, an antibody, a hybrid antibody, an antibody fragment, a siRNA, an antisense RNA, an aptamer, a protein, or a peptide. In exemplary embodiments, the cell is a cell of a nervous system, such as a neuronal or glial cell, a striatal cell, a cerebellar cell, a hippocampal cell, a hypothalamic cell, a cortical cell, a dopaminergic cell, a spinal cord cell. In other embodiments the cell is a neuron such as a dopaminergic neuron or a medium spiny neuron.

In an exemplary embodiment, the effect of cocaine on the translational profile of dopaminergic striatonigral and striatopallidal cells is obtained. In related embodiments the effects of acute cocaine administration and the effects of chronic cocaine administration is obtained. In specific embodiments, the gene translation changes following acute cocaine treatment comprise those identified in Table 15. In related specific embodiments, the gene translation changes following chronic cocaine treatment comprise those identified in Table 16.
4) Disease Screening, Diagnostics, Prognostics, and Theranostics
One embodiment of this invention is to establish a translational profile and molecular phenotype for a tissue or cell type taken from a subject to be screened for, suspected of having, or presenting with a particular disease or disorder, using the methods described herein. In this aspect, markers associated with and/or indicative of a particular disease or disorder are identified. In one embodiment, in a manner similar to a biopsy, a subject's tissue can be removed for sampling. In another embodiment, a sampling of cerebrospinal fluid (CSF) will be obtained from the subject. In other embodiments, any bodily fluid can be obtained from the subject. Following the isolation of desired tissue or fluid, a molecularly tagged ribosomal protein is introduced with the regulatory elements required for a cell-type specific expression. The translational profile and molecular phenotype is established using the methods described herein. The resulting profile and phenotype is then be compared to a profile and phenotype obtained using said methods from a subject or subjects not having or presenting with the particular disease or disorder, i.e. a reference cell, tissue or subject. The results can be used for diagnostics, prognostic, or theranostic purposes in relation to a disease or disorder. The disease or disorder to be detected, diagnosed, prognosed or theranosed, can be any disease named herein, or previously undescribed diseases and disorders.
In certain embodiments, BAC-mediated expression can be utilized to drive expression of a molecularly tagged ribosomal protein in a particular cell type. Several BACarrays have been created and collected that, based on expression of the endogenously regulated gene, have restricted patterns of expression that are directly relevant to several diseases or disorders ( FIG. 1 )
5) Screening for Modulatory Agents
Another embodiment of this invention is to screen for candidate agents for modulation of a desired modulatory (antagonistic, agonistic, synergistic modulation) activity. In certain embodiments a vehicle or candidate agent is administered in a single or repeated dose to either a cell type or a subject. Following administration a translational profile is established for the cell type of interest based on the methods disclosed herein. The profile and phenotypes from those dosed with candidate agent are compared and related to the profile and phenotype from those dosed with a vehicle (i.e. reference sample). A determination is made whether a candidate agent modulates translation and phenotype of one or more mRNA isolated from the cell type of interest, thereby screening said candidate agent for translational modulation. In some embodiments the candidate agent may be a candidate therapeutic agent or drug. In other embodiments, the candidate agent may be a toxin. In some embodiments the candidate agent may be but is not limited to a small molecule, an antibody, hybrid antibody or antibody fragment, a siRNA, an antisense RNA, an aptamer, a protein therapeutic, or a peptide.
6) Therapeutic Target Screening and Selection
Another aspect of this invention is to identify candidate therapeutic targets for a disease or disorder. In certain embodiments this would entail transforming a cell or creating an organism expressing a molecularly tagged ribosomal protein and exposing the cell or organism to a pertuthation or stimulus. mRNA transcripts selectively down regulated or up regulated would be potential targets for ameliorating the pertuthation. In other embodiments, a translational profile from a cell from a subject with a disease or disorder, a cell that has been perturbed, etc will be compared to an otherwise normal or unperturbed cell. The differential translational profile will allow for identification of potential therapeutic targets.
In one embodiment, any mRNA found to be upregulated in a cell type of interest associated with a disease or disorder, for example in but not limited to a striatal, hippocampal, cortical, cerebellar, spinal cord, hypothalamic, pineal, retinal, auditory, olfactory, vestibular, or brain stem cell may represent a target for antagonism. That is, the protein encoded by the identified mRNA would be a therapeutic target for which antagonists could be developed. In one exemplary embodiment, the target of interest is Gpr6. Gpr6 represents a target for which antagonists can be screened for and developed as a therapeutic for Parkinson's disease, due to its overexpression.
In a similar manner, an mRNA found to be downregulated in a cell type of interest associated with a disease or disorder, as above, could represent a target for agonism. That is, the protein encoded by the identified mRNA would be a therapeutic target for which agonists could be developed.
7) Personalized Medicine
In one embodiment, a method is provided for assessing whether a subject in need thereof is amenable to a therapeutic agent, comprising determining a translational profile for a cell type, cell sub-type or tissue from the subject, determining if the translational profile is predictive of treatment with one or more therapeutic agents, and identifying the one or more therapeutic agents to administer to the subject.
In another embodiment, a method is provided for screening one or more therapeutic agents in a subject in need thereof comprising, administering one or more therapeutic agents to a subject, determining a translational profile for a cell type, cell sub-type or tissue from the subject, comparing the translational profile obtained to one or more reference profiles that indicate a positive or negative prognosis, and determining the treatment should continue or be modified based on the comparison. In a further embodiment, it is determined that a different treatment modality (e.g., different therapeutic agent should be administered, such as for example, a second line drug, that is shown to be amenable to the translational profile observed for a first line drug).
8) Co-Regulated Gene Sets
In one embodiment, a method to identify a co-regulated gene set for a known function or a candidate function for a novel gene is provided. The method comprises determining the translational profiles for a plurality of cell types that express a gene associated with a specific function, comparing the translational profiles to determine what additional genes are similarly regulated thereby identifying a co-regulated gene set involved in the function.
In further embodiments the function can be a cellular function or a cellular process. In other related embodiments the method is applied to determine a co-regulated gene set involved in myelination, excitatory neural transmission, motor function, cellular migration, cellular adhesion, cellular infiltration, processing of noxious stimuli, processing of sensory stimuli, visual processing, auditory processing, olfactory processing, vestibular control, regulation of feeding and satiety, regulation of wakefulness and sleep, or the regulation of reward behavior.
In one exemplary embodiment, the method is applied to determine a co-regulated gene set involved in myelination comprising determining the translation profiles for a plurality of cells that express the myelin basic protein (Mbp). In a related embodiment the gene set comprises one or more of the genes listed in Table 9.
This method can be useful in identifying gene sets for known functions or candidate functions for novel gene products, since in many cases the cohorts of co-regulated genes can include genes with well known functions
Kits
In a further aspect, the present invention provides kits. In certain embodiments the kit contains reagents for determining the presence, absence, and/or differential presence, of one or more markers indicative of a disease, disorder, and/or pharmacologic, genetic, or environmental manipulation. The disease could be, but is not limited to a neurodegenerative, neuropsychiatric, neurodevelopmental disorder in a sample from an individual suspected of having a susceptibility to such a disease or disorder. In another embodiment, the disease or disorder is a proliferative disease such as a cancer. In other embodiments the kit is utilized to identify co-regulated gene sets for a particular biological function. Biological functions are described herein.
In one embodiment, the kit contains a customized set of clones, vectors, molecular tags from which to choose, for molecular labeling and materials such as CDs, instructions for use, and other reference guides that would allow the individual to choose the correct clone/vector for the cell type, tissue, molecular pathway, or circuit of choice. In another embodiment the kit contains a recombinant vector encoding a nucleic acid sequence which encodes a ribosomal protein and a detectable tag operably linked to a regulatory region. In particular embodiments, the kit can contain a customized BACarray clone collection relevant for a particular disease or disorder.
In one embodiment a kit includes a recombinant vector engineered to express a nucleic acid sequence encoding a ribosomal protein and a detectable tag operably linked to a regulatory region containing sequences endogenous to a genomic locus of a gene of interest. In a related embodiment the ribosomal protein is fused in-frame to a detectable tag. In certain embodiments, the recombinant vector is a BAC. The gene of interested can be selected from but is not limited to one that expresses in a striatal cell, cerebellar cell, cortical cell, hypothalamic cell, hippocampal cell, brainstem cell, and a spinal cord cell. In exemplary embodiments the tag is eGFP and the ribosomal protein is L10a.
EXAMPLES
The methodology reported here provides an enabling technology for translational profiling and molecular phenotyping. Examples allow for defining the distinguishing molecular characteristics of closely related or critical cell types. Examples also demonstrate that methods described herein can be employed to analyze physiological adaptations of specific cell types in vitro, in vivo, ex vivo, or in situ.
In various embodiments, compositions and methods feature methodology, which readily and reproducibly identify translated mRNAs in any cell type or tissue of interest. This methodology involves expression of a molecularly tagged ribosomal transgene, which enables tagging of polysomes for isolation, purification and identification of mRNA, in specific populations. In some embodiments, this can be achieved using Bacterial Artificial Chromosome (BAC) transgenic mice, allowing translational profiling and molecular phenotyping from whole animals
The following examples are offered to illustrate, but not to limit the claimed invention. Some embodiments of the present invention have been shown and described herein, but it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention.
Example 1
Molecular Tagging of a Ribosomal Proteins
mRNAs translated into protein are at one point usually attached to a ribosome or a polyribosome complex (polysomes). Any tag, not limited to those named herein, fused to a ribosomal protein allows for isolation of bound mRNAS. eGFP fused to the N-terminus of the large subunit ribosomal protein L10a, hereafter eGFP-L10a, was utilized in this and ensuing examples, but is by no ways limiting to the tens, hundreds, thousands, or even more tag-protein combinations that can be utilized.
FIG. 2 :( a ) A schematic is presented to illustrate affinity purification of eGFP-tagged Polysomes (originating from the target cell population) using anti-GFP antibody-coated beads (Schematic in a). Fusions of Enhanced Green Fluorescent Protein (eGFP) with ribosomal proteins were screened for efficient incorporation into polysomes to provide a tag for all translated cellular mRNAs. ( b ): A BAC carrying the eGFP-L10a fusion protein was transfected into HEK293T cells. The eGFP-L10a fusion protein's nucleolar and cytoplasmic localization was consistent with incorporation into intact ribosomes and immuno-electron microscopy data demonstrated its presence on polysome complexes. The figure displays transmission electron micrographs of anti-GFP coated magnetic beads after incubation with extracts taken from HEK293T cells transfected with an empty vector (left panel) or the eGFP-L10a construct (right panel); images acquired at 50,000× magnification, inserts enlarged by a factor of 2.3×.
There was no gross alteration in cell physiology or growth rate was evident in eGFP-L10a transfected cells, as seen by fluorescence microscopy of eGFP-L10a expression in cells. HEK293T cells grown on coverslips were transiently transfected, grown for two days, fixed with paraformaldehyde, and mounted with media containing 4′,6-diamidino-2phenylindo (DAPI) to stain DNA.
Example 2
Isolation, Purification and Analysis of Ribosome-mRNA Complexes In Vitro
Rapid immunoaffinity purification of polysomes was achieved from HEK293T cells transiently transfected with the eGFP-L10a transgene but not mock transfected cells. HEK293T cells transfected with eGFP-L10a were homogenized in lysis buffer. The solubilized and clarified lysate was loaded onto a linear density (20-50% w/w) gradient of sucrose and centrifuged for 2 hours at 4° C. using a Beckman SW41 rotor at 40,000 r. p. m. (200,000×g). 750 μl fractions were collected as absorbance at 254 nm was monitored with an ISCO UA-6 UV detector.
Immunoaffinity purification of polysomes from transfected cell cultures (in which approximately 30% of cells expressed eGFP-L10a) gave an approximate 10% overall co-purification of untagged ribosomal proteins and ribosomal RNA, and led to the recovery of only translated mRNAs (Table 3).





TABLE 3



Total RNA yields from cultured cell BACarray purifications.

HEK293T cells were transiently transfected with eGFP (mock

transfection) or eGFP-L10a constructs. Transfection efficiency was

approximately 30%. Plates of different sizes were used to grow mock

or eGFP-L10a-transfected cells, which is reflected in different input

RNA amounts. Input and immunoprecipitated (bound) RNA were

purified and the quantity and purity of RNA were determined using a

Bioanalyzer 2100 (Agilent Technologies).









Total RNA
Total RNA
Yield


Transfection
Bound (ng)
Input (ng)
(%)











eGFP (Mock)
61
33,320
0.2


eGFP-L10a
6,117
56,630
10.8


































FIG. 3 displays immunoprecipitation of translated mRNAs from transfected cells. HEK293T cells were transiently transfected (˜30% efficiency) with eGFP (mock) or eGFP-L10a constructs and grown in medium alone (no treatment, NT) or in medium supplemented with 100 μg/ml ferric ammonium citrate [pH 7.0] (iron) for 36 hours. (a) Induction of the iron-storage protein Ferritin was seen after 36 hours of iron treatment. (b) The post-mitochondrial supernatants of untreated or iron-treated cells were loaded onto linear sucrose gradients (20-50% w/w). After velocity sedimentation, fractions (direction of sedimentation noted by arrow) were collected while UV absorbance (254 nm) was being measured. A representative trace is shown, as profiles from non-treated and iron-treated lysates looked nearly identical. Non-polysome and polysome gradient fractions were generated as indicated. To avoid Ferritin mRNAs associated with mRNPs, only heavier polysomes (with greater than 4 ribosomes) were included in the polysome fraction. (c) After iron treatment, as expected, a shift of Ferritin heavy chain mRNA (Fth1) out of non-polysome fractions into polysome fractions was observed, as determined by reverse transcription followed by quantitative PCR of total RNA purified from non-polysome and polysome gradient fractions (range of fold-change in non-polysome fraction: 0.16-0.22; range of fold-change in polysome fraction: 1.83-2.15). (d) Immunoprecipitation were performed from non-polysome or polysome gradient fractions and 0.2% of the Input (IN), 0.02% of the unbound (UB), and 1% of the bound (IP) samples were loaded onto gels for immunoblot analysis with eGFP or Rpl7 antibodies; * indicates the presence of a weak Rpl7 band upon much longer exposure. eGFP-L10a was recovered equally well from non-polysome and polysome fractions with or without iron treatment. Rpl7 was recovered equally well from untreated or iron-treated polysome fractions. Rpl7 was not present in the non-polysome fraction, presumably because, unlike the overexpressed eGFP-Rpl10a, it was all incorporated into polysomes. As expected from the lack of Rpl7 (and thus assembled ribosomes) in the non-polysome fraction, immunoprecipitation from non-polysome fractions did not pull down any RNA above background, indicating that the immunoprecipitation was specific to translated messages. (e) To determine if the translating ribosome affinity purification (TRAP) methodology could faithfully reflect the changes observed in c, a direct eGFP immunoprecipitation of post-mitochondrial supernatants (whole cell lysates, unfractionated) was performed. 0.5% of input (IN), 0.5% of the unbound fraction (UB), and 1.0% of the bound (IP) samples were loaded onto gels for immunoblot analysis with eGFP (top), Rpl10a (middle), or Rpl7 (bottom) antibodies. eGFP-L10a was recovered equally well from untreated and iron-treated samples, as was all eGFP from mock samples; * indicates the presence of a light eGFP-L10a band upon longer exposure. No endogenous Rpl10a or Rpl7 was recovered in the bound (IP) fraction of mock samples, while endogenous Rpl10a and Rpl7 were both recovered in the bound (IP) fraction of untreated and iron-treated samples. The reduced recovery of endogenous Rpl10a versus endogenous Rpl7 in the immunoprecipitation likely reflects competition between endogenous Rpl10a and eGFP-L10a for incorporation into ribosomes. (f) The iron-induced fold-change in Fth1 mRNA levels relative to Actb mRNA levels in samples immunoprecipitated (IP) from either polysomes or from whole cell lysates (range of fold-change in polysome IP samples: 2.01-2.43; range of fold-change in direct IP samples: 1.80-1.93) was similar to the change observed in the polysome gradient fraction before immunoprecipitation (c).
Methods
Standard methods for immunoblotting were used. Antibodies were used as follows: GFP detection: JL-8, Clontech (Mountain View, Calif.), 1:2,000 in 5% non-fat milk/PBST (phosphate buffered saline-0.05% Tween-20]; Rpl7 detection: NB200-308, Novus Biologicals (Littleton, Colo.), 1:2,000 in 5% IgG-free bovine serum albumin/PBS-T; Rpl10a detection: H00004736-M01, Abnova Corporation (Taipei City, Taiwan), 1:2,000 in 5% IgG-free bovine serum albumin/PBS-T; Ferritin detection: 65077, MP Biomedicals (Solon, Ohio), 1:1,500 in 0.2% I-block (Applied Biosystems, Foster City, Calif.)/PBS-T; β-Actin detection: Ab8224, Abcam (Cambridge, Mass.), 1:2,500 in 5% nonfat milk/PBS-T.
Example 3
Characterization and Analysis of BACarray Transgenic Mice
1) Overview
Comparative analysis of BACarray data can provide important mechanistic insights into complex biological systems. BACarray translational profiling permits comprehensive studies of translated mRNAs in genetically defined cell populations, and their responses to physiological perturbations. To establish the generality of this approach, BACarray translational profiles for twenty four distinct and diverse CNS cell populations are presented here. Identification of cell-specific and enriched transcripts, previously not identified in whole tissue microarray studies are provided as examples to illustrate the added value of comparative analysis of these large datasets. The BAC transgenic strategy has been applied (Heintz, 2004; Yang et al., 1997) to provide high resolution anatomical data and BAC vectors for the design of genetic studies of specific, morphologically defined cells in the CNS (Gong et al., 2003) (www.gensat.org). Heiman et al. have reported the development of the BACarray translational profiling methodology for use in discovery of the complement of proteins synthesized in any genetically defined cell population. Here, the methodology is used to generate BACarray transgenic mice for a wide variety of anatomically and genetically defined cell types in the mammalian brain. In some embodiments, such a collection can provide a resource that will allow detailed molecular phenotyping of CNS cell types at specified developmental stages, and in response to a wide variety of pharmacological, genetic or behavioral alterations. The mice and data presented confirm the generality of the BACarray approach and provide a resource for studies of the molecular bases for cellular diversity in the mammalian brain.
The generation of BACarray translational profiles of specific CNS cell types requires targeting of the eGFP-L10a ribosomal protein fusion to desired CNS cell types, affinity purification of polysomal RNAs from these cell types, and interrogation of the resultant mRNA populations using massively parallel analytical techniques. To provide a resource for comparative analysis of diverse CNS cell types, described in this examples is: the application of this strategy to generate and characterize further BACarray transgenic lines, to isolate and characterize mRNA populations from twenty four cell types targeted in these lines, to analyze these data relative to one another, and to archive and present the BACarray transgenic lines and their associated anatomic and microarray data.
2) Selection of Drivers to Target Specific CNS Cell Types
To confirm the general applicability of the translating ribosome affinity purification (TRAP) methodology, and to obtain initial data concerning the depth of information one can obtain with this approach across multiple cell types, BACs reported by the Gene Expression Nervous System Atlas (GENSAT) Project (Gong et al, 2003; S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007); both incorporated in their entirety) were chosen to specifically target a wide range of neurons and glia from different structures throughout the CNS.
3) BACarray Mice
To genetically target expression of the eGFP-L10a fusion protein to defined CNS cell populations in vivo, BAC transgenic mice were created ( FIG. 4 represents the BACarray strategy; FIGS. 5 and 6 represent engineering of BAC vectors and the creation of mice carrying BACs). To tag mRNAs in specific cell types of the mouse, cell specific cis and trans regulatory elements and known transcriptional units were utilized as described herein and in (Gong et al. Nature Vol 425, 2003). Mouse lines with specific expression in different subsets of cells in the cerebral cortex ( FIG. 7 ) or hypothalamus ( FIG. 8 ) from BAC array lines are presented by way of example. Detailed anatomic characterization of selected BACarray transgenic mouse lines is presented below.
4) Anatomic Characterization of BACarray Transgenic Mouse Lines
Detailed anatomic studies were conducted for BACarray transgenic mouse lines as displayed in FIG. 9 . For each line, transgene expression was assayed by immunohistochemistry (IHC) using an antibody against eGFP. IHC data from serial coronal sections of the whole brain were collected, scanned at high resolution and mounted for inspection. All sections were processed equivalently (Neuroscience Associates) so that apparent differences in staining intensity accurately reflect differences in transgene expression level. The figure shows thumbnail images for each of the 24 cell populations selected for microarray analysis, and high resolution data to illustrate the morphology evident from IHC analysis of eGFPL10a expression. The regions covered in this characterization include the cerebellum (panels 1-9), the spinal cord (10), the basal forebrain and corpus striatum (11-14), the brainstem (15), and cerebral cortex (16-25).
For well characterized cell types, anatomic confirmation of the cells targeted for BACarray analysis was facilitated. For example, given the well described cytoarchitecture of the cerebellum, BACarray lines for Purkinje cells (Pcp2, panel 1), granule cells (Neurod1, panel 2), Golgi neurons (Grm2, panel 3), unipolar brush cells (Grp, panel 5), Bergmann glia (Sept4, panel 8), and astrocytes (Aldh111, panel 9) were easily identified from the IHC data presented. Purkinje cells can be recognized in the eGFP-L10a IHC data by their large cell bodies and molecular layer dendrites, granule cells by their small size, dense packing, and location in the granule layer, and Bergmann glial cells by their morphology, radial projections, and close proximity to Purkinje cells. IHC analysis of eGFP-L10a fusion proteins in these well known cell types using these regulatory regions allow for identification of well known and previously unknown cell types. The expression of the eGFP-L10a transgene from each BAC driver is regionally correct, conforming both to the literature and to the expectation given the anatomic data presented in GENSTAT and for these cell types the distribution of the eGFPL10a fusion protein, while more limited than that of soluble eGFP, is sufficient to provide enough anatomic detail to unambiguously identify these well described CNS cell types.
Many BACarray lines, the eGFP-L10a fusion protein is detected in multiple structures within the brain. An example is the characterization of fusion protein IHC in cholinergic cell populations targeted in the Chat BACarray lines. In this case expression is clear in brainstem motor neurons, spinal cord motor neurons, neurons of the corpus striatum, basal forebrain projection neurons, and neurons of the medial habenula. As detailed below, BACarray translational profiles for four of these cholinergic cell populations were collected by separately dissecting the spinal cord, brainstem, corpus striatum and basal forebrain prior to affinity purification of the eGFPL10a tagged polysome populations. Since specifically expressed genes are often found in distinct cell types in physically separable brain structures, several lines offer opportunities for analysis of many cell types not included in this example. Thus, the eGFP-L10a fusion protein is abundant in hippocampal CA1 cells in the Cck BACarray line, allowing translational profiling of mRNAs expressed in this cell type (panel 25). Creation of a BACarray anatomic database allows the user to browse through serial brain sections for each of the lines presented here to determine whether a cell type of interest can be analyzed in one of the BACarray lines presented in this example.
The figure also shows that projection neurons in the cerebrum can be identified by their pyramidal shape, and broadly classified by their laminar specificity, dendritic arbor, and axonal targets. Lines which clearly label the large pyramidal cells of layers 6 (Ntsrl, panel 16), 5b (Glt25d2, panel 17), and 5a (Etv1, panel 18) have also been reproduced. Morphometric studies provide additional data indicating that the GENSAT eGFP lines and eGFP-L10a BACarray lines target similar cortical pyramidal cell populations FIG. 10 shows that BACarray cortical pyramidal cells have the same laminar distribution as eGFP GENSTAT lines. The figure shows: A) Graph (mean+/−SE) of the distance of eGFP+ cell somas from the pial surface for the BACarray and GENSAT eGFP lines for each pyramidal cell BAC driver. The depth of the cells was consistent between both lines for each driver. B) The percentage of cells in 100 micron bins is shown as a histogram of the distribution of cell depths for each line in A. The BACarray line and eGFP line for each driver had overlapping distributions of cell depths.
FIG. 11 shows that in many BACarray lines, the presumed cellular identity of the targeted lines was confirmed using double immunofluorescence for the eGFP-L10a fusion protein and well characterized cell type specific markers. In some cases, the cell type specific markers corresponded with the BAC drivers chosen for modification (Olig2, Aldh111, Grm2, Chat). In other cases, commonly used markers which have been well characterized for specific cell types were used, as seen in panel B. In most cases, these studies established that BAC drivers limited expression to well-defined cell populations. There were also several BACarray lines in which the transgene is expressed in two or more cell types. For example, the immunofluorescence (IF) analysis of the Lypd6 BACarray line revealed that eGFP-L10a is found in all Pvalb positive and NeuN negative interneurons of the cerebellar molecular layer, suggesting that this line is valuable for analyses of both stellate and basket cells. Finally, in certain lines it is apparent that the eGFP-L10a transgene is expressed in a only a subset of a particular cell type. For instance, as seen in panel B, in the Grp BACarray line the eGFP-L10a fusion protein is restricted to the subpopulation of unipolar brush cells (Nunzi et al., 2002) which are immunoreactive for Grml but not Calb2 (calretinin).
BACarray lines whose expression did not conform to readily identified cell types, were also analyzed by IF analysis to provide data concerning the broad classification of cell populations targeted. For example, in the cerebral cortex of the Cort BACarray line, Calb 1 was detected in nearly 50% of eGFP-L10a positive cells, Pvalb was found in less than 5% of these cells, and Calb2 was not detected. Characterization of the fusion protein in the cortex of Pnoc BACarray mice revealed that the majority of eGFP-L10a positive cells in the superficial layers of the cerebral cortex are multipolar and are GABA positive, although some cells in deeper layers of cortex are GABA negative and appear to have a single apical dendrite. The multipolar cells in this case are often positive for Calb2, but not Calb1 or Pvalb. Both IHC and IF studies of the cortex of the Cck BACarray line clearly demonstrate that eGFP-L10a is detected in small neurons positive for Calb1 but not Pvalb or Calb2, as well as in pyramidal cells (data not shown), consistent with previous ISH data (www.stjudebgem.org; www.brain-map.org) (Lein et al., 2007; Magdalen et al., 2006).
In addition to neuronal cell types, three BACarray lines for glial cell types were generated which were analyzed in both cerebellar and cortical tissue. These glial cell types included astrocytes, mature oligodendrocytes, and a mixed oligodendroglial line that included mature oligodendrocytes and oligodendrocyte progenitors (also called synantocytes or polydendrocytes) (Butt et al., 2005). Astrocytes were targeted using a BAC for the gene Aldh111 that has previously been described as astrocyte specific (Anthony and Heintz, 2007; Cahoy et al., 2008). This BAC drove transgene expression in both Gfap+ (reactive) and Gfap− astrocytes, as well as Bergmann glia. It did not express in Ng2+ oligodendrocyte progenitors, nor in Cnp+ myelinating oligodendrocytes. In contrast, a BAC for the O1ig2 transcription factor directed expression specifically into both the Ng2+ and Cnp+ oligodendrocyte lineage cells. Finally, a BACarray line for Cmtm5 expressed specifically, albeit weakly, in mature (Cnp+) oligodendrocytes. With these three lines, the translational profile of the three major classes of glia across the CNS can be examined.
In cases of relatively weakly expressing lines, such as Cmtm5, new drivers can be selected to more effectively target the same cell type. Studies with a mature oligodendrocyte line (Cnp JD368) have demonstrated improved RNA yield and data quality from a more strongly expressing transgene ( FIG. 12 ).
5) Methods
BAC Modification, Transgenesis, and Animal Husbandry: BACs from
Table 4 were modified as described to insert an eGFP-L10a fusion protein into the translation start site of the driver gene (Gong et al., 2002; Gong et al., 2003). Founders and subsequent generations were bred to either Swiss-Webster or c57b1/6 wildtype mice. Lines were maintained as trans-heterozygotes.





TABLE 4



List of BACs utilized for transgenesis

Included are the list of abbreviations used throughout for each gene,

and the ID for the BAC clone that was modified.





Abbreviation
Full Gene Name
BAC



Sept4
Septin 4
RP23-21 N23

Aldh111
Aldehyde Dehydrogenase 1 family,
RP23-7N19


member L1

Cmbm5
CKLF-like MARVEL transmembrane
RP24-317F19


domain containing 5

C ck
Cholecystokinin
RP23-234l17

C hat
Choline Acetyltransferase
RP23-431D9

C ort
Cortistatin
RP23-281A14

Drd2
Dopamine receptor 2
RP23-161H15

Drd1
Dopamine receptor D1A
RP23-47M2

Grp
Gastrin-Releasing Peptide
RP23-179M10

Grm 2
Glutamate Receptor, Metabotropic 2
RP23-335E12

Glt25d2
Glycosyltransferase 25 Domain-containing
RP23-160M1


2

Lypd6
LY6/PLAUR domain containing 6
RP23-14O24

NeuroD1
Neurogenic Differentiation 1
RP24-151C22

Ntsr1
Neurotensin Receptor 1
RP23-314D14

Olig2
Oligodendrocyte Transcription Factor 2
RP23-356P18

Pnoc
Prepronociceptin
RP23-264L8

Pcp2
Purkinje Cell Protein-2
RP24-186D18

Etv1
Ets1 Variant Gene 1
RP23-250K4











































Immunohistochemistry: Six to twelve week old mice were euthanized with CO2 and perfused transcardially with phosphate buffered saline (PBS) pH 7.4 followed by 4% paraformaldehyde in PBS. For diaminobenzidine tetrahydrochloride (DAB) immunohistochemistry, fixed brains were treated overnight with 20% glycerol and 2% dimethylsulfoxide to prevent freeze-artifacts. Multiple brains (up to 25 per block) were embedded in a gelatin matrix using MultiBrain™ Technology (NeuroScience Associates, Knoxville, Tenn.). After curing, the block was rapidly frozen to −70° C. by immersion in a mixture of isopentane and crushed dry ice, and mounted on a freezing stage of an AO 860 sliding microtome. The MultiBrain™ block was sectioned coronally at 40 microns. All sections were collected sequentially into the wells of a 4×6 plate filled with Antigen Preserve solution (50% PBS pH 7.0, 50% Ethylene glycol, 1% Polyvinyl Pyrrolidone).
After blocking with hydrogen peroxide and serum, the sections were incubated with a 1:75,000 solution of Goat anti-eGFP serum (Heiman et al) overnight at room temperature. Following extensive washing, the sections were incubated with biotinylated secondary antibody (Anti Goat IgG, Vector Labs, Burlingame, Calif.), washed again, and incubated with avidin-biotin-HRP (Vectastain elite ABC kit, Vector Labs, Burlingame, Calif.) according to the manufacturer's instructions. Sections were again washed and incubated with DAB and hydrogen peroxide until fully developed. Finally, developed sections were mounted on gelatinized (subbed) glass slides, air dried, dehydrated in alcohols, cleared in xylene and coverslipped. Images were acquired with a Zeiss Axioskop2 microscope with a 10× (1.5 NA) objective using an automated x,y stage (Marzhauser scan8) controlled by a PC with Zeiss KS400 software running custom macros. Wildtype brains showed no labeling.
For fluorescence immunohistochemistry, fixed brains were cryoprotected in 30% sucrose in phosphate buffered saline (PBS), frozen, cut to 40 micron serial floating sections by cryostat, and stored in PBS 0.1% Sodium Azide at 4° C. until use. Sections were blocked in PBS with 5% normal donkey serum, 0.25% triton for 30 minutes, then incubated overnight with primary antibodies (Table 5). Sections were washed in PBS, exposed to appropriate Alexa dye conjugated secondary antibodies (Molecular Probes/Invitrogen, Carlsbad, Calif.) for 90 minutes, washed, then mounted. All images were acquired with a Zeiss Inverted LSM 510 laser scanning confocal microscope, with a Z thickness of 2 microns. Z-stacks through the sections were acquired to confirm colocalization.





TABLE 5



Antibodies, product numbers, and sources used for immunohistochemistry.






Symbol
Antibody Name
Product Number
Source



EGFP
Goat Anti-EGFP
NA
(Heiman et al.)

EGFP
Chicken Anti-EGFP
AB19370
Abeam, Cambridge Ma.

NEUN
Neuronal Nuclei
MAB377
Chemicon, Temecula, Ca.

OLIG2
Oligodendrocyte transcription factor 2
AB9610
Chemicon, Temecula, Ca.

CSPG4
Ng2 Proteoglycan
AB53420
Chemicon, Temecula, Ca.

CHAT
Choline Acetyl-Transferase
AB143
Chemicon, Temecula, Ca.

GRM2/GRM3
metabotropic glutamate receptor 2&3
AB1553
Chemicon, Temecula, Ca.

GRM1
metabotropic glutamate receptor 1
AB1551
Chemicon, Temecula, Ca.

CNP1
Cnpase
MAB326R
Chemicon, Temecula, Ca.

CALB1
Calbindin
300
Swant, Bellinzona, CH

CALB2
Calretinin
6B3
Swant, Bellinzona, CH

PVALB
Parvalbumin
Pv28
Swant, Bellinzona, CH

GFAP
Glial Fibrillary acidic protein
Z0334
Dako, Denmark

S100
S100
Z0311
Dako, Denmark

GLUL
Glutamine Synthetase
G2781
Sigma-Aldrich, St Louis, Mo.

SLC18a3
Vesicular Acetylcholine Transporter
sc-7717
Santa Cruz Biotech, Santa Cruz, Ca.

ALDH1L1
10-Formyltetrahydrofolate
NA
a gift from Dr. Robert Cook


Dehydrogenase

—
GABA
A2052
Sigma-Aldrich, St Louis, Mo.







































Fixed Sections: For fixed sections, BACarray transgenic mice were deeply anesthetized with pentobarbitol or 50 mg/ml Nembutal and transcardially perfused with 10m1 of phosphate buffered saline (PBS) followed by 40 ml of 4% paraformaldehyde (PFA) in PBS. Brains were dissected and post-fixed at room temperature for exactly 1 h with 4% PFA in PBS. After fixation, brains were washed 3 times in PBS and incubated in 5% weight/volume (w/v) sucrose in PBS at 4° C. for 1 hour with gentle agitation. Brains were then incubated for 24 h in 15% w/v sucrose in PBS at 4° C. with gentle agitation and for 24 h in 30% w/v sucrose in PBS at 4° C. with gentle agitation. Brains were placed in an embedding mold filled with Neg-50 embedding medium (Richard Allan Scientific, Kalamazoo, Mich.) for 1 hour at room temperature, and were subsequently incubated on dry ice for 1 hour to freeze the embedding medium. Brains were then transferred to and stored at −80° C. until sectioned. 12 μm sagittal sections were cut, mounted on glass slides, and kept overnight at −20° C., then transferred to −80° C. until used for immunohistochemistry.
Before use sections were thawed and dried at room temperature for 20 min, washed with PBS, and incubated in 0.2% H2O2/PBS at room temperature for 30 min to quench endogenous peroxidase activity. Sections were washed with PBS, permeabilized with PBS/0.05%Tween-20, and blocked with Image-it FX signal enhancer (Invitrogen Corporation, Carlsbad, Calif.) for 30 min at room temperature. Sections were washed with PBS/0.05%Tween-20 and blocked again with 2% donkey serum/0.1% fish gelatin/PBS/0.05% Tween-20. Sections were then incubated overnight at 4° C. with primary antibodies. The next day, sections were washed with PBS/0.05%Tween-20 and incubated with anti-rabbit Superpicture HRP Polymer Detection Kit (Invitrogen Corporation, Carlsbad, Calif.) for 1 hat room temperature. Sections were washed with PBS/0.05%Tween-20 and a fluorescent HRP substrate, Tyramide-AlexaFluor 546 conjugate (from TSA kit #13, Invitrogen Corporation, Carlsbad, Calif.), was deposited on the slides. Slides were washed again with PBS/0.05% Tween-20, mounted with Prolong Gold Antifade (Invitrogen Corporation, Carlsbad, Calif.), dried overnight, and fluorescence was visualized on a Zeiss LSM510 confocal microscope (Carl Zeiss, Thornwood, N.Y.).
Fresh Frozen Sections: For all nuclear immunohistochemistry (Anti-Atrx, Anti-PML, Anti-γH2A.X), fresh frozen brain sections were used. For fresh frozen brain sections, BACarray mice were euthanized with CO2, decapitated, and their brains removed into ice-cold PBS. Brains were then embedded in Neg50, placed on dry ice for an hour, and stored until needed at −80° C. Before sectioning, frozen blocks of tissue were equilibrated at −20° C., and 16 um sections were cut on a Leica cryostat. Slides with fresh frozen brain sections were stored at −80° C. or −20° C. until further use.
Prior to use, sections were allowed to air-dry at room temperature for 15 minutes, then fixed with 1% freshly made PFA in 1× PBS for 10 minutes at room temperature. Next, slides were washed three times with 1× PBS, and permeabilized for 10 minutes at room temperature with 0.05% Triton X-100 in PBS. After permeabilization, tissue sections were blocked using either 5% horse serum or 5% goat serum in PBS for at least one hour. Sections were incubated with primary antibodies overnight in a humidified chamber at 4oC, washed three times with PBS, and incubated at room temperature for one hour with appropriate secondary antibodies. After incubation with secondary antibodies, sections were incubated for 10 minutes with the nuclear stain TO-PRO-3 (Invitrogen Corp., Carlsbad, Calif.), washed three times with PBS, and coverslipped with Aquamount mounting medium (Lerner Laboratories, Pittsburgh, Pa.). Images were collected on an upright Zeiss Axioplan LSM 510 laser scanning confocal microscope (Carl Zeiss MicroImaging, Inc., Thornwood, N.Y.) using 40×/1.2, water immersion objective, 63×/1.4 oil immersion, and 100×/1.4 oil immersion lenses.
Quantification of laminar position of cortical pyramidal cells: Anti-eGFP immunohistochemistry with DAB was performed on 20 micron sagittal sections from the Etv1 TS88, Glt25d2 DU9, and Ntsr1 TS16 BACarray lines as described (Gong et al), and images were acquired as above. Corresponding digital images of the adult sagittal sections were downloaded from gensat.org for lines expressing eGFP from the same BACs. Sections containing motor cortex (corresponding to Paxinos section 111) (Paxinos and Franklin, 2001) were imported into ImageJ (rsb.info.nih.gov/ij). The distance from the apical tip of the soma to the pial surface was measured using the ‘straight line selection’ tool. The apical tip was defined as the site at which the apical dendrite and the cell body converge. Only cells with a clearly visible apical dendrite and a uniformly stained soma were measured. At least 50 cells were measured from each image. All measurements were then converted from pixels to microns using the following scale: 1 pixel=1.33 microns.
In situ hybridization: IMAGE consortium clones containing sequences from genes of interest were purchased from Open Biosystems (Table 6). Probes were synthesized by linearization of plasmid with appropriate restriction enzyme, template purification with Qiagen PCR purification kit, followed by in vitro transcription with appropriate enzyme (T3 or T7) using DIG RNA labeling kit (Roche, Basel, CH). Labeled RNA was purified with ProbeQuant G-50 microcolumns (GE Healthcare), and assayed for quality and quantity with a Bioanalyzer, following manufacturer's instructions (Agilent Technologies, Santa Clara Calif.).





TABLE 6



List of plasmids of in situ hybridization studies:








Genbank
Open Biosystems #
Gene Name
Enzymes










PNOC





Gene ID

B230118H07Rik
BC025075
MMM1013-7513748
RIKEN cDNA B230118H07 gene
Sal I cut, T7 polymerase

Chd4
BC058578
MMM1013-9201124
chromodomain helicase DNA
Sal I cut, T7 polymerase




binding protein 4

Cidea
AA061879
EMM1002-1115733
cell death-inducing DNA
Eco RI cut, T3 polymerase




fragmentation factor, alpha




subunit-like effector A

Crabp1
BC065787
MMM1013-9202137
cellular retinoic acid binding
Eco RI cut, T3 polymerase




protein I

Ddef1
BC094581
MMM1013-98479313
development and differentiation
Eco RI cut, T3 polymerase




enhancing

Igf1
BC012409
MMM1013-65370
insulin-like growth factor 1
Sal I cut, T7 polymerase

GRM2

Gene ID

Lypd1
CA328245
EMM1002-6960067
RIKEN cDNA 2700050C12 gene
Eco RI cut, T3 polymerase

Slc6a5, GlyT2
BM941867
EMM1032-584726
solute carrier family 6
Eco RI cut, T3 polymerase




(neurotransmitter transporter,




glycine), member 5

Amn
AA023455
EMM1002-1077429
amnionless
Eco RI cut, T3 polymerase

Penk1
AA098193
EMM1002-1183311
preproenkephalin 1
Eco RI cut, T7 polymerase

Gcqr
BC031885
MMM1013-7511930
glucagon receptor
Sal I cut, T7 polymerase

Ceacam 10
BC003346
MMM1013-62963
CEA-related cell adhesion
Sal I cut, T7 polymerase




molecule 10



ISH was conducted on 11 genes enriched in either Grm2 positive granule cell layer interneurons, or Pnoc positive neurons of cortex, using plasmids ordered from Open Biosystems. Plasmids were sequenced to confirm the accuracy and orientation of the ESTs. Plasmids were linearized with either Sal I or Eco RI and Dig labeled RNA was transcribed with either T3 or T7 polymerase to create anti-sense cRNA probe.














































Adult mice were processed as above, and brains were cut on cryostat into 20 micron sections and mounted onto Fisher Superfrost Plus slides. Tissue was washed, then post fixed for 20 minutes in 4% paraformaldehyde PBS, permeabilized with 0.05% Triton X100, digested with 50 ug/ml Proteinase K in TE for 10 minutes, and acetylated for 10 minutes in 0.1M TEA with 0.25% acetic anhydride. Sections were prehybridized in Atlas hybridization chambers (Clontech, Mountain View, Calif.) in 5×SSC, 2.5× Denhardt's solution, with 500 ug/ml sheared denatured salmon sperm for 30 minutes, then hybridized overnight with labeled riboprobe. Slides were washed for 5 minutes at 65° C. with 5×SSC, then for 1 hour with 0.2×SSC at 68° C., and for 5 minutes at room temperature. Slides were washed in TBS (100 mM Tris HCl, 150 nM NaCl, pH 7.5) twice for five minutes, blocked with 10% Roche blocking solution in TBS for one hour, incubated with primary antibodies for 1 hour in the same solution (Sheep anti-Dig, alkaline phosphatase conjugated, Roche 11 093 274 910) and Goat anti-eGFP where appropriate, washed with TBS, and developed with NBT/BCIP or HNPP/Fast Red following manufacturers protocols (Roche). For eGFP/ISH double fluorescence, sections were then incubated with alexa-488 donkey anti-goat antibody (Invitrogen) for one hour before counterstaining with DAPI, washing and coverslipping. Images were acquired with Zeiss Inverted LSM 510 laser scanning confocal microscope.
Example 4
Isolation and Purification of Ribosome-mRNA Complexes In Vivo
Improved procedures for rapid extraction and immunoaffinity purification of the eGFP-tagged functional polyribosome complexes from in vivo intact brain tissue were developed and optimized (A. Alexa, J. Rahnenfuhrer, T. Lengauer, Bioinformatics 22, 1600-7 (2006)). Highly purified RNA and protein was consistently obtained from BACarray mice. Key steps of the purification protocol included rapid manual dissection and homogenization of the tissue in question, inclusion of magnesium and cycloheximide in the lysis buffer to maintain ribosomal subunits on mRNA during purification, inhibition of endogenous RNase activity, solubilization of rough endoplasmic reticulum-bound polysomes under nondenaturing conditions, use of high-affinity anti-eGFP antibodies, and the addition of high-salt washes after immunoaffinity purification to reduce background. FIG. 13 displays purification of eGFP-tagged L10a and co-purification of untagged ribosomal protein L7 from D1 BACarray animals but not wild-type littermates in (a) (D1, samples from D1 BACarray mice; WT, samples from wild-type littermates; In, 1% Input; UB, 1% Unbound; IP, 6.5% Immunoaffinity purified sample). eGFP-L10a signal is only present in the D1 IP lane because the IP samples were more concentrated relative to In and UB. In (b) purification of 18 S and 28 S rRNA from D1 BACarray transgenic animals (top panel) but not wild type littermates (bottom panel) as detected by Bioanalyzer PicoChips (Agilent Technologies) is shown. 28 S rRNA runs at ˜47 sec, 18 S rRNA runs at ˜43 sec, and the marker peak runs at ˜23 sec.
Following immunoprecipitation of ribosomal complexes, electron microscopy was utilized to visualize the ribosomes. Aliquots of anti-GFP coated magnetic beads were fixed in 2.5% glutaraldehyde/0.1M cacodylate [pH 7.4] on ice. The bead pellet was post-fixed with 1% osmium tetroxide in the same buffer on ice. After treatment with 0.5% aqueous uranyl acetate at room temperature, the specimen was dehydrated with graded alcohol (70, 90, 100%) and treated with propylene oxide before embedding in Embed 812 resin. The resin was polymerized in a 60° C. oven for 2-3 days. Silver sections were cut with a Dupont diamond knife on a Reichert-Jung UltraCut E ultramicrotome. The sections were collected on copper grids, doubly stained with saturated, aqueous uranyl acetate and lead citrate before examination with a Jeol 100cx electron microscope (JEOL, Peabody, Mass.) operated at 80 kV ( FIG. 14 ).
1) Generation of Monoclonal Antibodies
All immunoprecipitation except for the Drd1 and Drd2 lines, which used the Goat Anti-eGFP described in later examples, were done using two monoclonal anti-eGFP antibodies (clones 19C8 and 19F7) specifically generated for this purpose at the Monoclonal Antibody Core Facility at Memorial Sloan-Kettering cancer center. Mice were immunized with purified GST-eGFP fusion protein and several rounds of screening were performed to identify clones which functioned well in immunoprecipitation assays. Initially, monoclonal supernatants were tested by ELISA using 96 well plates coated with eGFP purified from transiently transfected 293T cells. Next, positive clones were screened in immunoprecipitation assays, again using the eGFP purified from transfected 293T cells. Finally, positive clones which strongly immunoprecipitated eGFP from lysates from a transgenic mouse line expressing eGFP under the BAC driver of interest were identified.
2) Immunoprecipitation of Polyribosomes
Immunoprecipitation of polyribosomes and isolation of mRNA was done as described in detail below, except goat anti-eGFP antibody was substituted with a mix of two monoclonal eGFP antibodies (19C8, 19F7). Three to six mice for each replicated sample were euthanized with CO2 and distinct brain regions (cerebellum, cortex, corpus striatum, basal forebrain, brainstem, or spinal cord) were dissected Each cell population was assayed in triplicate. RNA quality control, amplification and hybridization were done as described by Heiman et al. For consistency across cell types, 15 ngs of total RNA were amplified for each sample.
Example 5
Analysis of mRNA from BACarray Mice 1) Preparation of mRNA
For mRNA purification, mice were decapitated and the specific tissue of interest was quickly dissected from mice brains. Pooled tissue was immediately collected in ice-cold dissection buffer and homogenized in ice-cold polysome extraction buffer (10 mM HEPES [pH 7.4], 150 mM KCl, 5 mM MgCl2, 0.5 mM dithiothreitol, 100 μg/ml cycloheximide, protease inhibitors, and recombinant RNase inhibitors) using a motor-driven Teflon-glass homogenizer. Homogenates were centrifuged for 10 minutes at 2,000×g, 4° C., to pellet large cell debris, and NP-40 or IGEPAL (EMD Biosciences, San Diego, Calif.; Sigma, St. Louis, Mo.) and DHPC (Avanti Polar Lipids, Alabaster, Ala.) were added to the supernatant at a final concentration of 1% and 30 mM, respectively. After incubation on ice for 5 minutes, the clarified lysate is centrifuged for 10 minutes at 13,000×g to pellet unsolubilized material. Goat anti-GFP (custom made) coated protein G Dynal magnetic beads (Invitrogen Corporation, Carlsbad, Calif.) are added to the supernatant and the mixture was incubated at 4° C. with end-over-end rotation for 30 minutes. Beads were subsequently collected on a magnetic rack, washed three times with high-salt polysome wash buffer (10 mM HEPES [pH 7.4], 350 mM KCl, 5 mM MgCl2, 1% NP-40, 0.5 mM dithiothreitol, 100 μg/ml cycloheximide) and immediately placed in TriZol-LS reagent (Invitrogen Corporation, Carlsbad Calif.) and chloroform to extract the bound rRNA and mRNA from polysomes. After extraction, RNA was precipitated with sodium acetate and Glycoblue (Ambion, Austin, Tex.) in isopropanol overnight at −80° C., washed twice with 70% ethanol, resuspended in water, and further purified using an Rneasy Micro Kit (Qiagen, Valencia, Calif.) with in-column DNase digestion. Purified samples were analyzed using a Bioanalyzer (Agilent Technologies, Santa Clara, Calif.) in order to assess mRNA quantity and quality, as reflected by rRNA levels and integrity.
After purification of RNA samples, 1.5 ul was used for quantification using a Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, Del.). Each sample was further analyzed with a Bioanalyzer (Agilent Technologies, Santa Clara, Calif.) to ensure that the quality of each RNA sample met standard criteria and that they were comparable to each other. Specifically, all RNA samples had a 260/280 ratio (Nanodrop) of at least 1.8, and a RNA Integrity Number (Bioanalyzer) of at least 7. Moreover, the quality of the samples was visually estimated using the Bioanalyzer readout to estimate the level of potential degradation or contamination for each sample.
2) Microarray Analysis of mRNA
Once these criteria were met, a total of 15 ng of RNA from each sample was amplified, biotinylated, and fragmented with the Affymetrix two-cycle amplification kit (Affymetrix, Santa Clara, Calif.). After amplification, samples were again quantified with a Nanodrop spectrophotometer, and 20 ug of amplified RNA was fragmented following the Affymetrix protocol. Amplified and fragmented samples were analyzed with a Bioanalyzer before hybridization to Affymetrix mouse 430 2.0 microarrays. All hybridizations were done according to standard Affymetrix protocols at the Rockefeller University Genome Array Center.
Tissue handling and RNA purification for all samples was as described. Purified RNA was converted to double-stranded cDNA using the SuperScript GeneChip Expression 3′Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, Calif.) and the GeneChip T7-Oligo(dT) Primer (Affymetrix, Santa Clara, Calif.). cDNA was used for the in vitro synthesis of cRNA using the MEGAscriptT7 Kit (Ambion, Austin, Tex.). cRNA was purified using the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, Calif.). 600 ng or less of clean cRNA was used in the second-cycle cDNA synthesis reaction using the SuperScript GeneChip Expression 3′-Amplification Reagents Two-Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, Calif.) and random primers (Affymetrix, Santa Clara, Calif.). The cDNA was purified using the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, Calif.). Purified cDNA was used for the in vitro synthesis of biotin-labeled cRNA using the GeneChip IVT Labeling Kit (Affymetrix, Santa Clara, Calif.). cRNA was purified using the GeneChip Sample Cleanup Module (Affymetrix, Santa Clara, Calif.) and fragmented into 35-200 base pair fragments using a magnesium acetate buffer (Affymetrix, Santa Clara, Calif.).
As controls, Affymetrix standard spike-in controls (eukaryotic hybridization kit) were used.
For hybridization procedures and parameters, 10 micrograms of labeled cRNA were hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays (http://www.affymetrix.com/products/arrays/specific/mouse430_2.affx) for 16 h at 45° C. The GeneChips were washed and stained according to the manufacturer's recommendations (Affymetrix, Santa Clara, Calif.) using the GeneChips Fluidics Station 450 (Affymetrix, Santa Clara, Calif.). This procedure included staining the chips with phycoerythrin-streptavidin, signal amplification by a second staining with biotinylated anti-streptavidin, and a third staining with phycoerythrin-streptavidin.
For array design, Affymetrix Mouse Genome 430 2.0 arrays were used in all experiments. Information regarding the array design and features can be found at http://www.affymetrix.com.
For measurement data and specifications, Mouse Genome 430 2.0 arrays were scanned using the GeneChip Scanner 3000 (Affymetrix, Santa Clara, Calif.). Three biological replicates were performed for each experiment. GeneChip CEL files were subjected to Harshlight analysis to detect if any blemishes were present on the GeneChips (http://asterion.rockefeller.edu/Harshlight/index2.html) (M. Suarez-Farinas, M. Pellegrino, K. M. Wittkowski, M. O. Magnasco, BMC Bioinformatics 6, 294 (2005)). Only GeneChips without major blemishes were used. GeneChip CEL files were imported into Genespring GX 7.3.1 (Agilent Technologies, Santa Clara, Calif.), processed with the GC-RMA algorithm, and expression values on each chip were normalized to that chip's 50 th percentile. Data were filtered to eliminate genes with intensities in the lower range. Only genes where more than one sample had a normalized intensity larger than 16 (4 in log2 scale) were kept in the analysis. Statistical analysis to determine which genes are differentially expressed in the different conditions was carried out using the Limma package from Bioconductor project (http://www.bioconductor.org).
Microarray Normalization and Analysis
MIAME compliant raw data are available from the APNRR server and GEO. Replicate array samples were normalized with quantile normalization (GCRMA). Data were filtered to remove those probesets with low signal (<50) from analysis, as well as those probesets identified as monoclonal background (Table 7), and replicate samples were averaged.





TABLE 7



List of Probesets excluded from analysis






Probeset
IP/UB WT
Symbol
Name








1428909_at
374.4
1200015M12Rik
novel

1428720_s_at
41.55
2010309G21Rik
novel

1453238_s_at
57.87
3930401B19Rik
novel

1435640_x_at
159
C8506T
novel

1425247_a_at
37.25
Igh-4
immunoglobulin heavy chain 4 iserum lgG′)

1425324_x_at
11.34
Igh-4
immunoglobulin heavy chain 4 iserum lgG′)

1424305_at
14.27
Igj
immunoglobulin joining chain

1452417_x_at
68.89
Igk-V28
immunoglobulin kappa chain variable 8




(VE)

1427455_x_at
60.27
Igk-V28
immunoglobulin kappa chain variable 8




(VE)

1427660_x_at
58.46
Igk-V28
immunoglobulin kappa chain variable 8




(VE)

1456162_x_at
861.2
Lmna
lamin AC

1455892_x_at
6.874
Lmna
lamin AC

1450009_at
12.58
Ltf
lactotransferrin

1456456_x_at
122.6
Mela
melanoma antigen

1427797_s_at
42.54
Mnda
myeloid cell nuclear differentiation antigen

1427798_x_at
313.97
Mnda
myeloid cell nuclear differentiation antigen

1452556_at
14.87
Mnda
myeloid cell nuclear differentiation antigen

1416957
57.24
Pou2af1
Pou domain, class 2, associating factor 1

1435697_a_at
9.079
Pscdbp
pleckstrin homology, Sec7 and coiled-coil




domains, binding protein

1436727_x_at
23.84
Sptlc1
serine palmitoyltransferase, long chain base




subunit 1

1435137_s_at
53.47

novel

1427932_s_at
26.78

novel



To identify mRNAs which interact with monoclonal antibodies or protein G dynabeads in the absence of eGFP, BACarray was performed on a wildtype mouse brain and compared to unbound whole brain RNA. The excluded probesets listed here are those for genes found to be highly enriched (>6 fold) in the wildtype IP/UB as well as enriched in multiple IPs from diverse regions and cell types.

















































Each IP was then compared to the unbound samples from the same tissue to calculate a ratio of IP/UB as a measure of ‘enrichment’. UB samples generally show little or no depletion of cell-specific RNA following IP, and UB samples from several different IPs from the same tissue were averaged for each comparison. UB samples from corpus striatum (Chat line) and neostriatum (Drd1 and Drd2 lines) were normalized together. IPs were globally normalized to UBs using the Affymetrix biotinylated spike in controls, to correct for any broad biases in scanning and hybridization For each cell type, Table 9 contains the IP/UB values for all genes with fold change >2 and p<0.05 by Welch's t-Test, with Benjamin and Hochberg FDR multiple testing correction, as calculated by Genespring GX version 7.3 (Agilent).
For three cell populations, a further ‘corrected enrichment’ was calculated. For the Bergmann glial line, Sept4, which also shows low level expression in mature oligodendrocytes, the corrected enrichment is the intersection of the IP/UB analysis described above with a comparison of the Bergman Glial line with the mature oligodendrocyte line, Cmtm5, using the same fold change and statistical criteria applied above. For unipolar brush cell line Grp, which also shows expression in some Bergmann glia, the corrected enrichment is the intersection of the IP/UB enrichment described above with a comparison of Grp with the Bergman glial line, using the same criteria. Finally, a substantial proportion of the RNA in the cerebellum is generated by granule cells, and thus the UB samples are highly enriched in granule cell RNA. Therefore, to identify granule cell genes, the ‘corrected enrichment’ was calculated by comparing granule cell IP to the average of all other cerebellar cell type IPs, using the same criteria as above.
Hierarchical clustering was performed in Genespring using the ‘condition tree’ function with a smoothed correlation metric on the GCRMA normalized data for the 20% of probesets with the highest coefficient of variation.
Shannon entropy was calculated in excel from GCRMA normalized values with the following procedure: After excluding probesets with no signal >100 in at least one sample, normalized expression measurements for each data set were categorized into 5 bins by log base 10 values (1-9, 10-99, 100-999, 1000-9999, 10000-99999), and Shannon entropy was calculated for each gene across 1) just the IP samples, 2) just the UB samples, and 3) across all samples using the following formula (Schneider, 2007).
M

H =−ΣPi log 2 Pi

i=1
The 10% of probesets with the highest and lowest entropy across all samples were analyzed using the BINGO plugin for the cytoscape software, using the full mouse Gene Ontologies, a p threshold of 0.01 on hypergeometic tests with Benjamin Hochberg FDR correction for multiple testing (Ashburner et al., 2000; Maere et al., 2005). Results from this analysis are substantially similar to those obtained using the EASE online implementation of Gene Ontologies and EASE statistic (Dennis et al., 2003).
Pearson's correlation with MBP was calculated in Genespring.
Comparative analysis of all cell types, and heat maps were generated with the R statistical software. Data were normalized as above. Then, for each cell type the total probeset list was filtered to remove those probesets with signal less than 50, or IP/UB values less than the average plus two standard deviations of the IP/UB values of the relevant negative control genes, or 1, whichever was lesser. Then, with this filtered list of genes, a fold change was calculated for this cell type versus all other samples, iteratively. For each comparison, the fold changes were ranked from highest to lowest, and these ranks were averaged across comparisons for a cell type. The top one hundred probesets from this average ranking were selected for further analysis.
To assess if the ribosomal immunoprecipitation is biased towards longer transcripts, the signal intensity versus transcript length for all probesets was plotted. No positive correlation between signal intensity and length was detected for any sample ( FIG. 15 ).
Example 6
BACarray Polysome Purification, RNA Extraction and Control Microarray Experiments
1) Overview
In total, twenty four cell populations in five regions were chosen to assay with immunoprecipitation (IP) and genome wide translational profile via microarray, using the translating ribosome affinity purification (TRAP) methodology of Heiman et al (2008). For each cell type, pooled microdissected tissue from three to six transgenic mice was used. As shown in FIG. 16 , this procedure yielded the purification of eGFP-ribosomal fusion protein along with cell specific mRNAs. The yield of total RNA from the initial IP is dependent on the number of labeled cells in the tissue and the intensity of the transgene expression within each cell, with RNA recoveries ranging from tens to thousands of nanograms per IP. RNA from the unbound (UB) fraction of the immunoprecipitation was harvested to measure the genes expressed in the dissected region as a whole. IP and UB mRNA were then amplified into labeled cRNA using standard protocols.
Labeled cRNAs from the IP and UB fractions were then hybridized onto Affymetrix mouse 430 2.0 microarrays. Samples derived from multiple independent pools of mice (replicates) were assayed for each cell type. FIG. 17 presents these data and illustrates that BACarray data are highly reproducible and cell-type specific
As shown in Panel A, replicates for the same cell type gave nearly identical genome wide translational profiles, confirming the results of Heiman et al, and extending this finding to many other cell types. The average Pearson's correlation between replicates for a given cell population from independently isolated samples was above 0.98 across all cell types. To determine whether the position of integration of the BACarray construct would influence the data, results obtained from independent BACarray founder lines prepared with the same engineered BAC were also examined, as presented in D. This analysis revealed that for independent founder lines targeting identical cell populations, the variation in translational profiles between lines was low, and no more extensive than that seen for replicate samples isolated from the same BACarray founder line (Panel D). Thus, the location of the transgene insertion into the genome had little global impact on the data obtained from the translating ribosome affinity purification (TRAP) methodology. Finally, four different monoclonal antibodies and one goat polyclonal against eGFP were tested. Each antibody immunoprecipitated comparable levels of mRNA for the BACarray lines tested; similar global gene translational profiles were obtained from each antibody tested in IPs from specific BACarray lines. A small number of probesets were consistently enriched in every BACarray dataset analyzed. Since these same probesets are also enriched in immunoprecipitates from control mice with no transgene expression, it was concluded that they represent background which have systematically eliminated from further analysis.
The enrichment for each mRNA immunoprecipitated from the targeted cell type (IP) versus its expression in the tissue sample dissected for the analysis (UB) was measured. The ratio of IP/UB was calculated, thereby identifying those genes which are highly enriched in each cell type. Panel B shows scatter plots for three representative cell types of the cerebellum. Differences are evident between the genome-wide translational profiles of IP samples compared to whole tissue (UB), with each cell population displaying a unique profile of thousands of specifically enriched genes on the microarray. As in Panel C, Venn diagrams constructed from the top 1000 most enriched probesets for each cell type can be used to illustrate this point. Thus, approximately 75% of these enriched probesets are not shared between cerebellar Purkinje cells, granule cells and unipolar brush cells, and only 52 of the probesets enriched in these three cell types versus whole cerebellum are shared between them. Furthermore, the primary data collected from these experiments has been deposited in the Gene Expression Omnibus (Edgar et al., 2002).
FIG. 18 presents the accuracy of this methodology to enrich for cell-specific genes. The BACarray data for known cell-specific markers (positive controls) for each cell type and the BACarray data for genes known to be expressed exclusively in other cell types (negative controls) were examined. Panel A shows a scatter plot of IP vs. UB for spinal cord motor neurons. Probesets for known markers of motor neurons with measurable signal on the array are clearly enriched in the IP sample, whereas probesets for glial cell-specific RNAs, that should not be present in these cells, are enriched in the UB sample. To establish the generality of this finding, the enrichment in the IP or UB sample was quantified by calculating an average ratio of IP/UB for positive and negative controls for each cell type where at least three positive controls could be found in the literature. As shown in Panel B, all IPs showed a clear enrichment for appropriate known markers, (Panel B, plotted in log base 2). Even for cell types with only one known marker (Pnoc positive interneurons, and Grp expressing unipolar brush cells), probesets for these genes were consistently and highly enriched in the IP. In the IPs with the lowest relative yield of RNA, such as those for mature oligodendrocytes (Panel B), and Cort expressing interneurons, background was proportionally higher, and enrichment was less robust.
Novel cell-specific markers for rare cell types using the BACarray approach were identified. Eleven genes predicted by BACarray to be enriched in either the Grm2 expressing interneurons of the granule cell layer (Golgi cells), or the Pnoc expressing cells of the cerebral cortex were screened. Using confocal microscopy, double immunofluorescence for both eGFP-L10a fusion protein and the ISH probes were evaluated. For the nine genes where ISH gave clear results, all were clearly overlapping with eGFP-L10a.
Panel C shows that in the case of cerebellar Golgi cells, there is a great deal of overlap between eGFP-L10a expression in the BACarray line and expression of the genes chosen for this analysis. This overlap confirms the specificity of the results obtained for this and other cell types. Nonetheless, the enrichment of a particular mRNA in the IP sample cannot be used to conclude that it is exclusively expressed in the cell type labeled in the BACarray transgenic line, or that it is expressed in all cells of that type. For example, the ISH databases (www.stjudebgem.org; www.brain-map.org) clearly indicate that Penk1 is expressed in scattered cells in both the granular and molecular layers of the adult cerebellum. Furthermore, as shown in panel 1, Penk1 mRNA does not appear to be expressed exclusively in those expressing Grm2. Finally, some mRNAs enriched in the BACarray data collected from Golgi cells were not detected using the fluorescence ISH technique, perhaps reflecting limited sensitivity of ISH for low expressed genes or the need for more rigorous probe design. Thus, although a clear result for Ceacam10 expression in granule layer interneurons is not evident in either of the ISH databases (www.stjudebgem.com; www brain-map.org), in both cases one can see scattered signal in this area that may indicate expression of this mRNA in cerebellar Golgi neurons.
In order to further validate the BACarray datasets, the enrichment of a variety of mRNAs isolated from the Chat (motor neuron) and Pcp2 (Purkinje cell) BACarray transgenic lines were measured with quantitative real time PCR (qRT-PCR) (Panel D). For all of the control genes tested, this methodology confirmed the BACarray results. For genes not previously known to be expressed in a specific cell type, results from qRTPCR demonstrated that seven out of the eight mRNAs assayed were in fact cell type enriched (Panel D). Moreover, despite a negative ISH result, qRT-PCR validated the expression of Ceacam10 in the cerebellum and its enrichment in Golgi cells (Panel D). In some cases, therefore, the translating ribosome affinity purification (TRAP) methodology appears to be more sensitive than ISH.
2) Quantitative PCR (qPCR) of Purified mRNA
In some experiments, 20 ng of purified RNA was used to produce cDNA with a NuGEN WT Ovation kit (NuGEN Technologies, San Carlos, Calif.) and the resulting cDNA was purified and quantified. 10 ng of cDNA was used for each real-time gene expression assay. Applied Biosystems (Foster City, Calif.) TaqMan pre-designed gene expression assays were used, following the manufacturer's instructions and using an Applied Biosystems 7900 Sequence Detection System.
Alternatively, in some experiments, cDNA was synthesized from 20 ng of total RNA from the three replicate IP and UB samples using M-MulV reverse transcriptase (M0253L), from New England Biolabs (Ipswich, Mass.), using oligo dT23VN as a primer, then purified with the Qiagen Quick PCR cleanup, following manufacturer's instructions (Qiagen, Valencia, Calif.).
Most primer sequences (Table 8) for qRT-PCR were obtained from Primer Bank (Wang and Seed, 2003). PCR was performed using Bio-Rad iQ syber green supermix following manufacturer's protocols (Biorad, Hercules, Calif.), with 500 nm final concentration of each primer. Cycling and quantitation were performed using Biorad iQ5 multiplex real-time detection hardware. PCR was carried out for 45 cycles (94°, 30 seconds, 63°, 30 seconds, 72°, 30 seconds), followed by a melt curve. Each replicate was assayed in triplicate. Conditions yielding dimers, as demonstrated by melt curve and/or gel electrophoresis were excluded from further analysis. Primers that did not yield product in at least 2 of 3 replicates prior to 35 cycles were excluded from further analysis. Data were normalized to B-actin (Overbergh et al., 1999) with the ddCT method, via iQ5's optical system software version 2, and averaged across replicates. All qPCR products were subcloned and sequenced to confirm accuracy of PCR. Microarray data were also normalized to B-actin for comparison purposes.





TABLE 8



List of qRT-PCR primers

qRT-PCR primer sequences were mostly from PrimerBank (Wang and Seed, 2003) or literature.

All are listed in 5′ to 3′ direction.






Gene
Forward Primer
Reverse Primer
Primerbank ID or Ref



B-Actin
agagggaaatcgtgcgtgac
caatagtgatgacctggccgt
(Overbergh et al., 1999)



Gfap
gtaaagactgtggagatgcgggatggtgagg
gtgctggtgtgggtgggaactgag




Chat
ccattgtgaagcggtttggg
gccaggcggttgtttagataca
26338049a1



Slc18a3
gtgaagataggcgtgctatttgc
gactgtggaggcgaacatgac
11096330a2



Cnp1
tgcttgatgataccaaccacg
gctgggcacagtctagtcg
6753476a3



Pcp2
tgcagggcgatcggatggaggag
tgaggggtgagcaggggttgagg




Rilp
ctgatgcggcaacctcagat
ttgagcaagaacacgttggct
30185833a1



Popdc3
tgactgaacacccactctgc
actgccacccataaaacctact
31745187a1



Kcnn1
ttgaaaagcgtaaacggctca
cagagcaaaagagcagagtga
14161696a1



Foxq1
aaattggaggtgttcgtccca
tccccgtctgagcctaagg
31560693a1



Cox7a1
gctctggtccggtcttttagc
gtactgggaggtcattgtcgg
6753504a1



Tpm2
aagtcgctgatagcctcagag
ggtctggtgtatctccacgttc
50190a1



Crygs
cagacttccgctcgtacctaa
tcgccctggggtaagatgt
6753532a1



Cfi
cttggctctccacttgagttc
ggagcgatgcgtgtatttctg
6671744a1

















































Example 7
Comparative Analysis of BACarray Data Collected from Many Cell Types
Previous examples show that the BACarray data accurately reflect expression of known positive and negative controls for each cell type and that these results can be confirmed by independent experimental analysis (Heiman et al.). This example illustrates the broad properties of these cells that could be inferred from comparative analysis of this large set of microarray data. The results of this analysis are presented in FIG. 19 . A hierarchical clustering of the GCRMA normalized data from all 24 IP and 6 UB samples using the 20% of probesets with the highest coefficient of variation was performed, as presented in Panel A. This unsupervised clustering essentially recapitulates the known biology of CNS cell types. Thus, the three populations of cortical projection neurons are more similar to one another than they are to cortical interneurons, Purkinje cells, or motor neurons. Astroglial BACarray data collected from different regions of the brain are, as expected, more similar to one another and to Bergmann glia than they are to oligodendrocytes. Oligodendroglia are more similar to each other than they are to any neuronal population, etc. These findings support the concept that cells with similar gene expression patterns share similar functions, and suggest that analysis of BACarray data will allow for the identification of those gene products responsible for the distinguishing characteristics of each cell type.
The diversity of translational profiles across neuronal types nearly rivals the diversity between neurons and glia. Although related cell subtypes, such as different motor neurons or the Drd1 and Drd2 medium spiny neurons, are clearly tightly clustered, many neuronal types (e.g. Purkinje cells) are not strongly clustered with any other cell type. This suggests that comparative analysis of BACarray translational profiles obtained from highly specialized cell types may yield insights into their biochemical properties. Finally, individual cell types did not generally cluster tightly with the UB samples from their tissue of origin. In fact, profiles from UB samples derived from different brain regions were loosely clustered together relative to the data obtained from specific CNS cell types, suggesting that microarray data produced from dissected whole brain regions are less informative than BACarray analysis of individual cell types.
To examine this point in more detail, the microarray data from total cerebellum to that of the cerebellar cell types analyzed in this study were compared. As can be seen in Panel B, any single cell type has fewer probesets detectable than the whole cerebellar sample, since the whole cerebellar sample represents an aggregate of different cell types. However, comparative analysis of the sum of the probesets detectable in each of the six individual cerebellar cell types and the results obtained from whole cerebellar tissue reveals over 4000 probesets that are undetectable in the microarray from whole cerebellum. These undetectable probesets tend to represent cell-type enriched genes. In fact, for rare cell types, up to 42% of the genes enriched in that cell may not be detectable at all in whole-tissue microarray studies. For detection of genes expressed in specific cell types within complex brain regions, therefore, the translating ribosome affinity purification (TRAP) methodology can be more sensitive than microarray analysis of dissected brain regions.
The increased sensitivity of the translating ribosome affinity purification (TRAP) methodology results in identification of more mRNAs in each cell type, yielding a more complete picture of the translational profile for each cell type, more information. To assess if this increased sensitivity in fact does gives better information, the Shannon entropy was calculated for each probeset across the six whole tissue samples, and across the twenty four individual cell populations (Fuhrman et al., 2000; Shannon and Weaver, 1969). Shannon entropy is a measure of information content that describes the complexity of a signal across samples, with values ranging from 0 (low information) to 2 (high information). Data are presented in FIG. 20 . Examples of probeset with low and high information are shown in Panel B. Shannon entropy measures of the information content in these samples reveals that the average Shannon entropy in cell type specific experiments (IP' s) is over twice as high as that calculated from microarray data of whole tissue samples (t-Test, p<0.0001, average entropy across all IPs: 0.88+/−0.002, whole tissue: 0.41+/−0.003). This analysis demonstrates that microarray data collected from specific cell types using the BACarray strategy can provide better information than traditional microarray studies of dissected brain tissues.
As those genes with high entropy measures across all samples are those that vary in the most complex manner between cell types, this entropy measure was applied to assess what fundamentally determines the differences between cell types in the nervous system. As seen in Panel C, the ten percent of the probesets with highest entropy and those with the lowest entropy were classified with Gene Ontologies and then searched for functional categories that were over-represented (Ashburner et al., 2000; Maere et al., 2005). According to this analysis, cell type diversity in the nervous system is driven primarily by the expression of cell surface proteins, such as channels and receptors, and also to some extent by the specific expression of transcription factors and calcium binding proteins. Genes with less information content tend to be those that that are more ubiquitously expressed, such as ribosomal and mitochondrial proteins. This is not to say that they do not vary—their expression can range often from two to five fold across cell types—but they vary less dramatically than the tens to thousands fold changes of many receptors and channels.
Table 9 is a comparative analysis of translational profiling to identify co-regulated genes that could encode the highly specialized properties of individual cell types. This analysis is can be useful in trying to identify gene sets for known functions or candidate functions for novel gene products, since in many cases these cohorts of co-regulated genes will include genes with well known functions. To test whether the BACarray data provided can yield productive results in this sort of query, a probeset for a gene known to be involved in myelination was selected—the myelin basic protein (Mbp). Its highest correlates were examined across all IP and UB samples. In the top 35 genes correlating with Mbp expression (min correlation, 0.86), 6 genes also known to be involved in myelination were identified, including Plp1, Cnp, Mog, Mal and Mobp, and another three genes previously identified in a proteomic screen of myelin components (Table 9).





TABLE 9



Genes correlating with myelin basic protein.






Probeset
Correlation
Symbol
Name








1419646_a_at
1
Mbp
myelin basic protein

1417275_at
0.968
Mal
myelin and lymphocyte protein, T-cell




differentiation protein

1451718_at
0.964
Plp
proteolipid protein (myelin)

1440902_at
0.958
Galnt5
UDP-N-acetyl-alpha-D-




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 5

1433785_at
0.958
Mobp
myelin-associated oligodendrocytic basic




protein

1436578_at
0.955
A330104H05Rik
novel

1428792_at
0.946
Bcas1
breast carcinoma amplified sequence 1

1439506_at
0.934
C11ORF9
novel



homolog

1426960_a_at
0.931
Fa2h
fatty acid 2-hydroxylase

1433543_at
0.926
Anln
anillin, actin binding protein (scraps homolog,




Drosophila )

1448768_at
0.921
Mog
myelin oligodendrocyte glycoprotein

1418086_at
0.917
Ppp1r14a
protein phosphatase 1, regulatory (inhibitor)




subunit 14A

1434094_at
0.914
6330530A05Rik
novel

1420760_s_at
0.911
Ndrg1
N-myc downstream regulated 1

1447807_s_at
0.905
Plekhh1
pleckstrin homology domain containing,




family H (with MyTH4 domain) member 1

1425546_a_at
0.905
Trf
transferrin

1436974_at
0.904
A230069A22Rik
novel

1450241_a_at
0.904
Evi2a
ecotropic viral integration site 2a

1418472_at
0.903
Aspa
aspartoacylase (aminoacylase) 2

1452834_at
0.9
2600010E01Rik
novel

1429909_at
0.894
4833411O04Rik
novel

1453009_at
0.893
1110060I01Rik
novel

1440813_s_at
0.89
Plxnb3
plexin B3

1437171_x_at
0.888
Gsn
gelsolin

1423871_at
0.887
BC014795
novel

1434399_at
0.887
Galnt6
UDP-N-acetyl-alpha-D-




galactosamine:polypeptide




N-acetylgalactosaminyltransferase 6

1435854_at
0.885
Tmem10
transmembrane protein 10

1416371_at
0.878
Apod
apolipoprotein D

1418406_at
0.877
Pde8a
cAMP-specific cyclic nucleotide




phosphodiesterase PDE8

1416003_at
0.875
Cldn11
claudin 11

1434606_at
0.872
Erbb3
v-erb-b2 erythroblastic leukemia viral




oncogene homolog 3 (avian)

1451932_a_at
0.872
Tsrc1
thrombospondin repeat containing 1

1418980_a_at
0.871
Cnp1
cyclic nucleotide phosphodiesterase 1

1416318_at
0.87
Serpinb1a
serine (or cysteine) proteinase inhibitor, clade




B, member 1a

1424468_s_at
0.868
D330037A14Rik
novel



Correlation of genes with a Mbp probeset (1419646_a_at) identifies known and putative novel myelination genes. Four of the first eleven genes correlated with Mbp represent genes for the known myelin components Mal, Plp, Mobp, and Mog. This coexpression suggests that novel genes highly correlated with Mbp (C11orf9, A330104H05Rik, Bcas1) may also be involved in myelination. Pearson's correlation. Only the first probeset for each gene is shown for those genes with multiple probesets on the array. In bold are genes identified in an independent proteomic screen of myelin components (Vanrobaeys et al., 2005).






































































FIG. 21 shows a comparative analysis of the individual cell samples to identify only those genes most highly specific for each population. An iterative comparison was performed: one-by-one, each sample was compared to each other sample in the dataset, and for each population, probesets were sorted by their average ranking across these comparisons. Data were then combined and clustered by expression the top one hundred ranked probesets for each population in a heatmap (Panel A). This heat map readily illustrates the extent to which distinct cell types are characterized by specific cohorts of genes. For example, cerebellar Purkinje cells are clearly distinguished by a group of genes that are not seen in any other cell types (Panel A). Thus none of the top twenty five most specific probesets observed in the Purkinje cell sample are found in any of the top twenty five most specific probesets for any of the other cell types. In contrast, Drd1 and Drd2 medium spiny neurons, two closely related cell types, co-express many genes that are not found in the other cell populations analyzed, yet they also express distinct subsets of genes that differentiate them (Heiman et al). Thus, comparative analysis of BACarray data can be used to characterize CNS cell populations with unique biochemical and physiological properties, and to distinguish between closely related cell types at the molecular and biochemical level.
As shown in the tables in Panel B, the top twenty five most specific probesets in each cell type include probesets for both well known cell-specific markers and novel, previously uncharacterized genes. For example, Pcp2, the calcium binding protein Calb1, the scaffolding/synaptic protein Homer3, and the transcription factor Ebf2, all of which are known to be specifically expressed in Purkinje cells (Malgaretti et al., 1997; Shiraishi et al., 2004; Wang et al., 1997), are among the most highly ranked probesets in the Pcp2 BACarray list. Mobp, one of the most abundant components of the CNS myelin sheath (Montague et al., 2006), is prominent in the Cmtm5 myelinating oligodendrocytes' list. The expression of Tcrb in deep layer cortical neurons (Nishiyori et al., 2004) is confirmed in the Ntsr1 BACarray data. The large number of uncharacterized genes with cell specific translation identified here provide an important resource for discovery of novel biochemical pathways operating in these cell types, or for the identification of new proteins operating in well known pathways. Finally, comparative analysis can reveal discrepancies that are not apparent from anatomical studies. For example, the most specific probesets for the Etv1 line identify several genes well-known to be expressed in lymphoid cells, suggesting that in this line the eGFP-L10a transgene may also be expressed in circulating cells in the CNS vasculature. Taken together, the data shown above demonstrate two important strengths of large-scale comparative analyses of BACarray data. First, molecular relationships between cell types can be easily established with hierarchical clustering; second, groups of genes that encode the biochemical functions of specific cell types can be identified using this sort of systematic comparative approach.
Example 8
Analysis of BACarray Data Collected From Spinal Motor Neurons
Due to their involvement in a variety of serious neurological disorders and severe, acute injuries, spinal cord motor neurons (MN) are among the most well studied cell types of the CNS. As such, they provide an opportunity to evaluate the BACarray data with the depth of the knowledge available for this cell type. In particular, a wealth of anatomical and physiological data available for MN, and the comprehensive studies of transcription factors involved in their development, allowing comparison of the BACarray data presented here with the published literature. As shown in FIG. 22 , in a single BACarray experiment, most of the MN expressed molecules that have been documented in prior studies are rediscovered. To perform this analysis, BACarray results were color coded as ‘expressed,’ enriched,' or ‘not expressed,’ as described in the methods. This classification was then compared to results reported in the adult rodent literature, color coded simply as either ‘expressed’ or ‘not expressed’ or left uncolored in cases where there were no studies or conflicting data. In most cases, where microarray probesets were present and informative, BACarray results agree well with the literature. Thus, it has been reported that MNs express glutamate receptors sensitive to AMPA, kainate, and NMDA (Rekling et al., 2000). These results suggest that the specific receptor subunits mediating these responses include Gria3 and 4, Grik2 and 4, and Grin1, 3a and 3b. Inhibition in MNs should be due the actions of the Glrα2 and GlrB glycine receptor subunits and both metabotropic (Gabbr1) and ionotropic GABAergic receptors, potentially composed of Gabrα2, α5, and β3 subunits. These data predict that MNs should respond to all classic neurotransmitters, including acetylcholine, via Chrnα4/β2 and/or Chrnα7 receptors, and serotonin, via the Htr1d receptor. In disagreement with prior immunohistochemical findings (Rekling et al., 2000), expression of Drd1 and or Drd2 in MNs was not detected. Moreover, transgenic mice for Drd1 and Drd2 do not show transgene expression in MNs, nor does the Allen Brain Atlas ISH show expression in brain stem MN, supporting the BACarray results.
MNs also express a variety of newly characterized receptors and orphan receptors. For example, BACarray data has successfully identified Grin3b as a MN specific gene encoding an NMDA subunit. This receptor was recently characterized as creating a unique glycine gated channel in MNs (Chatterton et al., 2002; Nishi et al., 2001). Several other genes enriched in MNs have also been identified which potentially encode for MN specific receptors that either have not been previously characterized in MNs or are entirely unstudied. Two that are particularly interesting are the vitamin D receptor and the orphan receptor P2rx11. Future studies investigating the role of these receptors in MN behavior may explain cases of reversible muscle weakness in patients with vitamin D deficiency (Whitaker et al., 2000; Ziambaras and Dagogo-Jack, 1997), or suggest new pathways important to MN function.
Example 9
Molecular Phenotyping and Translational Profiling of Cholinergic Motor Neurons and Purkinje Neurons
To determine whether the BACarray technique could be used for the characterization of other types of neurons, cholinergic and Purkinje cell-specific BACarray lines were produced, as presented in FIG. 23 . The cholinergic cell BACarray line was produced by placement of the eGFP-L10a transgene under the control of the choline acetyltransferase (Chat) locus, which is specifically expressed in cholinergic cells in the CNS. As expected from the published Chat expression pattern in the rat CNS (Oh et al., 1992), the Chat BACarray line DW167 showed highest eGFP-L10a expression in cholinergic cells of the dorsal striatum and ventral striatum (nucleus accumbens, olfactory tubercle, and islands of Calleja), basal forebrain, brain stem, spinal cord, and medial habenula (Panel a). eGFP expression was shown to be restricted to cholinergic cells in all of these structures, including motor neurons of the brain stem (Panel b), by indirect immunofluorescence staining for Chat. One exception to this colocalization was in the pedunculopontine and laterodorsal tegmental nuclei, where only a minority of cholinergic cells were labeled with eGFP.
The Purkinje cell BACarray line DR166 was produced by placement of the eGFP-L10a transgene under the control of the Purkinje cell protein 2 (Pcp2) locus, which is specifically expressed in cerebellar Purkinje cells of the CNS (Oberdick et al., 1988). The Pcp2 BACarray line showed eGFP-L10a expression that was restricted to cells that possessed a characteristic Purkinje cell morphology (Panel c). Restriction of eGFP-L10a expression to cerebellar Purkinje cells was confirmed by indirect immunofluorescent staining of Calbindin-D28K (Panel D), which in the cerebellum is specifically expressed in Purkinje cells (Nordquist et al., 1988). Array data were collected from brain stem cholinergic motor neurons using the Chat BACarray line and from Purkinje cells using the Pcp2 BACarray line. Replicate Chat BACarray samples gave nearly identical genome-wide translational profiles (average Pearson correlation=0.982), as was the case for replicate Pcp2
BACarray samples (average Pearson correlation=0.997). To provide a measure of the enrichment of each mRNA immunoprecipitated from the targeted cell type (IP) versus its expression in a reference sample (unbounds in immunoprecipitation), the ratio of the expression in each IP sample versus the reference sample was calculated. This comparison identified those genes which were highly enriched in each cell type versus a common reference sample. As expected from the analysis of D1 and D2 combined BACarray data versus whole brain minus, differences are evident between the genome-wide translational profiles of IP samples compared to the reference sample for all the individual samples analyzed: striatonigral (D1), striatopallidal (D2), brain stem cholinergic (Chat), and Purkinje cells (Pcp2). FIG. 24 shows that enrichment of cell-specific positive-control genes and exclusion of known negative-control genes (glial genes), were evident for each comparison (panels a-d). Venn diagrams constructed from the top 1,000 enriched probesets from this analysis (Tables 17-20) confirmed that the translational profiles of striatonigral and striatopallidal cells show greater similar to each other than they do to the translational profiles of either brain stem cholinergic cells or Purkinje cells (panels e-h).
Example 10
Molecular Phenotyping and Translational Profiling of the Striatum
1) Overview
This example presents a study of the specific properties of the striatum. This example illustrates one embodiment that can uncover unexpected molecular and physiological complexity in closely related and spatially adjacent CNS cell populations. The striatum is a subcortical part of the telencephalon. It is the major input station of the basal ganglia system. In this example, transgenic mice containing molecularly tagged ribosomal proteins in the striatonigral and striatopallidal cells of the striatum were created using standard BACarray techniques. Striatonigral and striatopallidal medium spiny neurons (MSNs), are intermixed, indistinguishable in somato-dendritic morphology, and of major interest due to their role in the etiology of various neurological diseases, including Parkinson's disease, schizophrenia, attention deficit hyperactivity disorder, drug addiction, and Huntington's disease. Striatonigral MSNs send projection axons directly to the output nuclei of the basal ganglia, i.e. the substantia nigra and the internal segment of the globus pallidus (the entopeduncular nucleus in rodents), while striatopallidal MSNs send projection axons to the external segment of the globus pallidus. Striatonigral MSNs are known to preferentially express the dopamine D1 receptor (Drd1; Drd1a) and the neuropeptides substance P (Tac1) and dynorphin (Pdyn), while striatopallidal MSNs preferentially express the dopamine D2 receptor (Drd2), the adenosine A2a receptor (Adora2a) and the neuropeptide enkephalin (Penk) (S. Gong et al., Nature 425, 917-25 (2003)).
2) Production of BACarray Striatal Mice
Mice containing molecularly tagged ribosomal proteins in the striatonigral and striatopallidal cells of the striatum were created. Homologous recombination in bacteria was used to place eGFP-L10a under the control of either the D2 receptor (striatopallidal) or D1 receptor (striatonigral) loci in the appropriate BACs. Mouse lines were generated by pronuclear injection of engineered BACarray DNA contracts into fertilized oocytes.
Mouse lines were screened by immunohistochemistry for appropriate expression of the transgene, as judged by known D1 and D2 receptor expression patterns. In the Drd2 line, 75.8% colocalization of pro-enkephalin with eGFP-L10a (n=204/269) was observed and in the Drd1a line 0% colocalization was observed (n=0/325). For the Drd2 line, this number is likely an underestimate, as heat-induced epitope retrieval in citrate buffer is needed for optimal staining using pro-enkephalin antibodies. Heat-induced epitope retrieval in the colocalization experiments was not utilized, however, because both eGFP fluorescence and the ability of various GFP antibodies to recognize eGFP are substantially diminished upon use of these epitope retrieval methods. Colocalization data for eGFP-L10a (direct eGFP fluorescence) and pro-enkephalin expression (immunohistochemical staining) is presented ( FIG. 25 ): (a) Immunohistochemistry to eGFP in adult sagittal sections from the D2 BACarray line CP101. (b) Characterization of D2 BACarray line CP101 striatal MSN cells: direct eGFP fluorescence (left panel with high-magnification image insert); enkephalin immunohistochemical staining (middle panel); merge (right panel, with 20 μm scale bar). (c) Immunohistochemistry to eGFP in adult sagittal sections from the D1 BACarray line CP73. (d) Characterization of D1 BACarray line CP73 striatal MSN cells: direct eGFP fluorescence (left panel); enkephalin immunohistochemical staining (middle panel); merge (right panel).
The D2 BACarray line showed highest transgenic eGFP-L10a expression in the dorsal and ventral striatum, olfactory tubercle, and hippocampus. In addition, expression of eGFP was seen in the substantia nigra pars compacta and ventral tegmental area of this line, as expected due to D2 autoreceptor expression in dopaminergic cells (panel a). The D1 BACarray line showed highest transgenic eGFP-L10a expression in the dorsal and ventral striatum, olfactory bulb, olfactory tubercle, and cortical layers 5 and 6 (panel c). As expected for a ribosomal protein fusion, direct visualization of eGFP fluorescence revealed localization of transgenic eGFP-L10a to the nucleoli and cytoplasm (panel b). eGFP co-localization with enkephalin expression (striatopallidal cell marker) was observed in striatal cells from the D2 BACarray line but not the D1 BACarray line (panels b and d), verifying correct BAC-mediated cell-type expression.
The polysome profile from D2 BACarray mouse striatal extract is presented in FIG. 26 as follows: Top: Post-mitochondrial striatal extract (S20) was loaded onto a linear sucrose gradient (20-50% w/w). After velocity sedimentation, fractions (direction of sedimentation noted by arrow) were collected as UV absorbance (254 nm) was measured. Bottom: gradient fractions were ethanol precipitated, resuspended in SDS-PAGE loading buffer, and Rp17 and eGFP-L10a content were assayed by Western blotting. Velocity sedimentation analysis of polysome complexes isolated from striatal extracts for both lines of BACarray mice confirmed incorporation of the eGFP-L10a fusion protein into functional polysomes in vivo.
3) BACarray Profiling of Striatonigral and Striatopallidan MSNs
A plurality of differentially expressed genes can be identified which characterize striatonigral and striatopallidal MSNs, including all previously known markers. Translation profiling and molecular Phenotyping was performed with immunoaffinity-purified mRNA from adult striatonigral or striatopallidal BACarray mice. Following two rounds of in vitro transcription, biotin-labeled antisense RNA (cRNA) was used to interrogate Affymetrix GeneChip Mouse Genome 430 2.0 arrays. For each cell type, data were collected from three independent biological replicates, each prepared from a cohort of 7 animals Analysis of immunoaffinity-purified samples revealed no bias for mRNA length or abundance ( FIG. 27 ). Lengths of transcripts were based on all available mouse curated RefSeq RNA sequences (ftp://ftp.ncbi.nih.gov/genomes/M_musculus/RNA). Where multiple transcript variants for a single gene were available, the longest one was chosen. RefSeq lengths were plotted against D1 (a) or D2 (b) BACarray IP normalized expression values. There was no correlation observed between transcript length and IP values. Striatal expression values for all Affymetrix Genechip probe sets were obtained by total RNA arrays from wild-type striatal tissue (data not shown). These values were plotted against (c) D1 BACarray or (d) D2 BACarray IP normalized values. As expected, higher expression in total striatum (no IP, wild type mice) correlates with higher D1 or D2 BACarray IP values. The few genes that showed modest expression in total striatum but had low IP values include known non-neuronal genes.
Comparative analysis of these data revealed that all 8 of the well-characterized, differentially expressed MSN markers were enriched using the BACarray approach: D2 (Drd2) (36.6×), adenosine 2a receptor (Adora2a) (13.2×), and enkephalin (Penk) (7.5×) were enriched in the striatopallidal BACarray sample, while D1 (Drd1a) (3.9×), substance P (Tac1) (3.6×), and dynorphin (Pdyn) (5.6×) were enriched in the striatonigral BACarray sample ( FIG. 28 and
Table 10). Four striatopallidal-enriched mRNAs (Adk, Plxdc1, BC004044, and Hist1h2bc) and six striatonigral-enriched mRNAs (Slc35d3, Zfp521, Ebf1, Stmn2, Gnb4, and Nrxn1) were confirmed as reported in a microarray study of FACS sorted MSNs (S. Magdaleno et al., PLoS Biol 4, e86 (2006)). However, the data identified approximately 70 additional striatopallidal enriched transcripts and over 150 additional striatonigral-enriched transcripts (
Table 10). To provide an initial test of the data, quantitative PCR studies were carried out using independent biological BACarray D1 and D2 samples and a different cDNA amplification procedure (A. Alexa, J. Rahnenfuhrer, T. Lengauer, Bioinformatics 22, 1600-7 (2006)). Differential expression of Eya1, Isl1, Gng2, and Crym in striatonigral MSNs, and Gpr6, Lhx8, Gpr88, Trpc4, and Tpm2 in striatopallidal MSNs was confirmed (Table 11 and Table 12). qPCR assays used were Gapdh: Mm99999915_gl, Drd2: Mm00438541_ml, Gpr6: Mm01701705_sl, Lhx8: Mm00802919_ml, Gpr88: Mm02620353_sl, Trpc4: Mm00444284_ml, Tpm2: Mm00437172_gl, Eya1: Mm00438796_ml, Tac1: Mm00436880_ml, Isl1: Mm00627860_ml, Gng2: Mm00726459_sl, Chrm4: Mm00432514_sl, Drd1a: Mm02620146_sl, Crym: Mm01281258_ml,Actb: Hs99999903_ml, and Fth1: Hs01694011_sl. Each assay was performed in quadruplicate and fold enrichment values were derived from the comparative Ct method (following Applied Biosystems recommendations), with each target amplification compared to a Gapdh or Actb reference amplification.





TABLE 10



Genes differentially translated

between striatonigral and striatopallidal cells.








Probe
Symbol
GenBank
FCH











1457424_at
Eya1
BB760085
32.49


1424606_at
Cplx3
BC024854
19.56


1418047_at
Neurod6
NM_009717
13.05


1428393_at
Nrn1
AK003046
12.21


1417560_at
Sfxn1
BB478992
11.99


1422720_at
Isl1
BQ176915
10.10


1450723_at
Isl1
BQ176915
9.44


1420388_at
Prss12
NM_008939
9.25


1419473_a_at
Cck
NM_031161
9.11


1447863_s_at
Nr4a2
BB322941
9.00


1419200_at
Fxyd7
NM_022007
7.90


1455034_at
Nr4a2
BB703394
7.52


1417559_at
Sfxn1
BB478992
7.42


1428642_at
Slc35d3
AK018094
7.32


1451499_at
Cadps2
AF000969
6.87


1450750_a_at
Nr4a2
NM_013613
6.44


1426852_x_at
Nov
X96585
6.44


1431339_a_at
Efhd2
AK007560
6.00


1428184_at
3110035E14Rik
BB348639
5.61


1416266_at
Pdyn
AF026537
5.59


1428157_at
Gng2
AV021455
5.08


1441388_at

BB428710
5.05


1437478_s_at
Efhd2
AA409309
5.04


1423280_at
Stmn2
BM115022
4.89


1428156_at
Gng2
AV021455
4.85


1421727_at
Eya1
NM_010164
4.84


1451322_at
Cmbl
BC024580
4.82


1423281_at
Stmn2
BM115022
4.73


1448293_at
Ebf1
BB125261
4.72


1416711_at
Tbr1
NM_009322
4.70


1422256_at
Sstr2
NM_009217
4.68


1416302_at
Ebf1
BB125261
4.55


1440361_at

BB272510
4.48


1455893_at
Rspo2
BG067392
4.35


1428664_at
Vip
AK018599
4.35


1419470_at
Gnb4
BI713933
4.32


1452779_at
3110006E14Rik
AK014009
4.26


1416301_a_at
Ebf1
BB125261
4.25


1436312_at
Ikzf1
AV317621
4.20


1424737_at
Thrsp
BC009165
4.16


1448366_at
Stx1a
NM_016801
4.14


1451620_at
C1ql3
BB768838
4.11


1448889_at
Slc38a4
NM_027052
4.08


1426851_a_at
Nov
X96585
4.07


1426318_at
Serpinb1b
AF426025
4.03


1423287_at
Cbln1
AA016422
4.01


1417343_at
Fxyd6
AB032010
3.99


1427017_at
Satb2
BB104560
3.92


1419469_at
Gnb4
BI713933
3.89


1455629_at
Drd1a
BE957273
3.87


1417400_at
Rai14
NM_030690
3.74


1423286_at
Cbln1
AA016422
3.71


1456051_at
Drd1a
BB282271
3.69


1419184_a_at
Fhl2
NM_010212
3.64


1434440_at
Gnai1
BQ174580
3.64


1418789_at
Sntg2
NM_133742
3.61


1416783_at
Tac1
NM_009311
3.61


1457440_at
Sstr4
BB451927
3.59


1418451_at
Gng2
BB522409
3.57


1442724_at
AW060763
AW120464
3.55


1455758_at
Prkcc
BM215011
3.52


1430306_a_at
Atp6v1c2
AK004157
3.48


1456515_s_at
Tcfl5
AV044715
3.43


1416953_at
Ctgf
NM_010217
3.40


1441917_s_at
Tmem40
BB468188
3.35


1437091_at
Accn4
AV323885
3.30


1436786_at
Sec14l3
AV024133
3.29


1429274_at
2310010M24Rik
AK009282
3.23


1421017_at
Nrg3
NM_008734
3.23


1416503_at
Lxn
NM_016753
3.22


1417933_at
Igfbp6
NM_008344
3.18


1427510_at
Gnai1
U38501
3.16


1436862_at
Doc2a
BB543070
3.13


1452065_at
Vstm2
BB085570
3.11


1448546_at
Rassf3
BB703307
3.08


1429175_at
Tmem178
AK014196
3.08


1418417_at
Msc
NM_010827
3.08


1448812_at
Hpcal1
NM_016677
3.07


1454691_at
Nrxn1
BB336165
3.04


1450042_at
Arx
BB322201
3.04


1446498_at
Il20ra
BB551879
3.03


1457984_at
Crh
BM933756
3.03


1438729_at
BB176347
BB331017
3.03


1440108_at
Foxp2
BM964154
2.99


1434695_at
Dtl
AV270035
2.97


1439632_at

BQ174397
2.96


1417288_at
Plekha2
NM_031257
2.93


1426937_at
6330406I15Rik
AK018128
2.92


1438624_x_at
Hs3st2
BB494451
2.85


1440849_at
6330417G04Rik
AV327187
2.85


1426738_at
Dgkz
BC014860
2.84


1428781_at
Dmkn
BI452905
2.83


1439789_at
Ebf1
BQ177189
2.83


1450055_at
Vsnl1
NM_012038
2.83


1417569_at
Ncald
BG071381
2.80


1437671_x_at
Prss23
BB378796
2.79


1444661_at
Gpr26
BB247791
2.79


1427975_at
Rasl10a
AK008807
2.77


1438112_at
9430021M05Rik
AA546727
2.76


1436787_x_at
Sec14l3
AV024133
2.73


1417579_x_at
Gmppa
NM_133708
2.72


1456146_at
2210411A11Rik
BI735554
2.72


1436503_at
BC048546
BF302511
2.71


1420720_at
Nptx2
NM_016789
2.70


1455925_at
Prdm8
AV349236
2.68


1448632_at
Psmb10
NM_013640
2.67


1426329_s_at
Baalc
AF371320
2.65


1448669_at
Dkk3
AK004853
2.65


1425090_s_at
Kcnc4
BC024837
2.64


1454959_s_at
Gnai1
BQ174580
2.63


1453558_at
4930504H06Rik
AK015697
2.63


1423757_x_at
Igfbp4
BC019836
2.62


1437405_a_at
Igfbp4
BB787243
2.59


1450059_at
Fancg
BG072083
2.54


1450708_at
Scg2
NM_009129
2.52


1429269_at
BC068157
BE992549
2.51


1422537_a_at
Id2
NM_010496
2.51


1417568_at
Ncald
BG071381
2.49


1435411_at

BE950834
2.49


1424966_at
Tmem40
BC019416
2.48


1438796_at
Nr4a3
BE692107
2.48


1416776_at
Crym
NM_016669
2.47


1437406_x_at
Igfbp4
BB787243
2.45


1423202_a_at
Ncor1
U22016
2.45


1444307_at

AW491448
2.44


1439573_at
Rtn4rl2
BE992565
2.43


1417605_s_at
Camk1
NM_133926
2.42


1425784_a_at
Olfm1
D78264
2.39


1455074_at
Efcab1
AV307860
2.39


1451342_at
Spon1
BC020531
2.38


1420955_at
Vsnl1
NM_012038
2.38


1417192_at
Tomm70a
NM_138599
2.38


1452169_a_at
Dgkz
BC014860
2.37


1418304_at
Pcdh21
NM_130878
2.37


1454770_at
Cckbr
AV221910
2.37


1440918_at

AW495511
2.37


1448754_at
Rbp1
NM_011254
2.37


1428240_at
Nrxn1
AK017578
2.36


1453245_at
9130024F11Rik
BB329046
2.36


1438231_at
Foxp2
AV322952
2.36


1438232_at
Foxp2
AV322952
2.35


1417578_a_at
Gmppa
NM_133708
2.34


1428077_at
Tmem163
AK011522
2.32


1453317_a_at
Khdrbs3
AK014353
2.32


1419551_s_at
Stk39
BG919998
2.32


1421144_at
Rpgrip1
NM_023879
2.32


1421926_at
Mapk11
AV329330
2.30


1415849_s_at
Stmn1
BC010581
2.30


1424763_at
1700027N10Rik
BC019423
2.27


1459847_x_at
Gfra2
AV354240
2.27


1449314_at
Zfpm2
NM_011766
2.26


1430634_a_at
Pfkp
BB076574
2.26


1457032_at
Ak5
BB546359
2.25


1453129_a_at
Rgs12
AK004813
2.24


1447669_s_at
Gng4
AV347903
2.24


1433909_at

AW048713
2.22


1424701_at
Pcdh20
BB528056
2.21


1435959_at
Arhgap15
BM246535
2.21


1417988_at
Resp18
NM_009049
2.21


1455885_at
Gna12
AV238106
2.21


1427054_s_at
Abi3bp
BC026627
2.20


1458112_at
Adarb2
BB527550
2.20


1427115_at
Myh3
M74753
2.19


1449556_at
H2-T23
NM_010398
2.18


1435176_a_at
Id2
BF019883
2.16


1422552_at
Rprm
NM_023396
2.15


1416131_s_at
C920006C10Rik
BB188557
2.15


1436609_a_at
Lrpap1
AV309553
2.14


1451332_at
Zfp521
BC021376
2.13


1452444_at
Napb
AU067119
2.13


1457277_at
BC038925
AI314927
2.13


1434243_s_at
Tomm70a
AV233564
2.12


1436592_at

BB453760
2.12


1448249_at
Gpd1
BC019391
2.11


1417604_at
Camk1
NM_133926
2.09


1443129_at

BB363699
2.09


1452741_s_at
Gpd2
BQ175968
2.08


1417960_at
Cpeb1
NM_007755
2.08


1418726_a_at
Tnnt2
NM_011619
2.08


1442887_at
Gpr39
BQ175060
2.07


1421176_at
Rasgrp1
BB354696
2.06


1428650_at
Tns1
AK003780
2.06


1428323_at
Gpd2
BQ175968
2.06


1425287_at
Zfp189
BC021326
2.06


1452974_at
Nol8
AK017551
2.05


1417038_at
8-Sep
NM_017380
2.03


1417943_at
Gng4
NM_010317
2.03


1448326_a_at
Crabp1
NM_013496
2.03


1416407_at
Pea15
AI323543
2.03


1436821_at
Plcxd3
BB293136
2.02


1426606_at
Crtac1
BB426194
2.01


1449429_at
Fkbp1b
NM_016863
2.01


1452731_x_at
LOC554327
BM195235
2.00


1425854_x_at
Tcrb-V13
U07661
2.00


1452936_at
Crtac1
AV341285
1.99


1448660_at
Arhgdig
NM_008113
1.98


1415897_a_at
Mgst1
BI150149
1.98


1431429_a_at
Arl4a
AK006286
1.98


1416004_at
Ywhah
NM_011738
1.97


1418982_at
Cebpa
BC011118
1.97


1418271_at
Bhlhb5
NM_021560
1.96


1449403_at
Pde9a
NM_008804
1.95


1449799_s_at
Pkp2
AA516617
1.94


1454745_at
Arhgap29
BG074320
1.94


1425051_at
Isoc1
AK010892
1.94


1455324_at
Plcxd2
BQ176176
1.94


1418792_at
Sh3gl2
AF326561
1.93


1437390_x_at
Stx1a
AV339210
1.93


1416133_at
C920006C10Rik
BB188557
1.93


1455443_at
Gdap1l1
BB733286
1.93


1433664_at
Ube2q2
BM202586
1.92


1431749_a_at
Rasgrp1
AK013548
1.91


1455374_at

BB332873
1.91


1426766_at
6330403K07Rik
AK018106
1.91


1445081_at
A930041I02Rik
BB335888
1.90


1455458_x_at
Gmppa
AU044197
1.89


1436182_at

BG092481
1.88


1425484_at
Tox
BB547854
1.87


1456954_at
Kcna6
BM119753
1.84


1417434_at
Gpd2
NM_010274
1.83


1454708_at
Ablim1
BG065289
1.83


1416008_at
Satb1
AV172776
1.81


1449839_at
Casp3
BG070529
1.80


1420858_at
Pkia
AK010212
1.79


1425006_a_at
Vrk1
BC016676
0.57


1437573_at

BF018351
0.57


1456434_x_at
Hspb8
BB764222
0.56


1452879_at
Synpo2
AI848603
0.55


1436238_at
Lgi3
AI841179
0.54


1431320_a_at
Myo5a
AK002362
0.54


1448845_at
Rpp25
NM_133982
0.53


1428574_a_at
Chn2
AK006398
0.53


1416967_at
Sox2
U31967
0.53


1445461_at

BB096245
0.53


1426572_at
Me2
BM235734
0.53


1423171_at
Gpr88
BE947345
0.53


1423424_at
Zic3
BB732077
0.52


1451236_at
Rerg
BC026463
0.52


1420533_at
Gucy1a3
AK004815
0.52


1419754_at
Myo5a
NM_010864
0.52


1455265_a_at
Rgs16
BB100249
0.52


1419717_at
Sema3e
NM_011348
0.52


1436946_s_at
Gng5
AV216608
0.51


1419738_a_at
Tpm2
AK003186
0.51


1439526_at
Me2
AV375160
0.51


1458951_at
Vrk1
AV341598
0.51


1441368_at

BB102769
0.50


1455753_at
C630035N08Rik
BQ176181
0.50


1436994_a_at
Hist1h1c
BB533903
0.49


1428283_at
Cyp2s1
AK004699
0.49


1420694_a_at
Dach1
BB522228
0.49


1454821_at
B3gat1
BB424673
0.49


1452540_a_at
Hist1h2bp
M25487
0.48


1456935_at
AI606473
AI606473
0.48


1419879_s_at
Trim25
AA960166
0.48


1426215_at
Ddc
AF071068
0.48


1420534_at
Gucy1a3
AK004815
0.48


1418072_at
Hist1h2bc
NM_023422
0.47


1426573_at
Me2
BM235734
0.47


1437231_at
Slitrk6
AV246497
0.47


1456858_at
Gpr149
BB075339
0.47


1442226_at
Sema3e
AV348197
0.47


1448839_at
Ankrd47
NM_030697
0.46


1422673_at
Prkcm
NM_008858
0.46


1443365_at
Htr4
BB431008
0.46


1438841_s_at
Arg2
AV002218
0.46


1434141_at
Gucy1a3
BG072799
0.46


1448415_a_at
Sema3b
NM_009153
0.45


1439614_at
Htr4
BB308379
0.45


1436913_at
Cdc14a
BB479310
0.45


1451033_a_at
Trpc4
BB271442
0.45


1449241_at
Klhl1
NM_053105
0.45


1460327_at
Gpr88
BE947345
0.45


1448162_at
Vcam1
BB250384
0.44


1446681_at
BB086117
AV327329
0.44


1450992_a_at
Meis1
AW547821
0.44


1449577_x_at
Tpm2
AK003186
0.44


1417688_at
BC004044
NM_030565
0.44


1435138_at
Tmem28
AV016797
0.43


1457648_x_at
BC004044
BB084182
0.42


1417306_at
Tyk2
NM_018793
0.42


1424854_at
Hist1h4i
BC019757
0.41


1443827_x_at
BC004044
BB375974
0.41


1418895_at
Skap2
NM_018773
0.41


1443372_at

BB417900
0.41


1440484_at
Unc5d
BB525782
0.41


1448276_at
Tspan4
NM_053082
0.40


1425028_a_at
Tpm2
BC024358
0.39


1447623_s_at
Prkcm
AV297026
0.39


1455739_at
EG245190
BB279146
0.39


1418355_at
Nucb2
NM_016773
0.38


1419517_at
Cnih3
NM_028408
0.38


1423477_at
Zic1
BB361162
0.38


1422860_at
Nts
NM_024435
0.38


1423367_at
Wnt7a
AK004683
0.37


1435479_at

BM055476
0.36


1437156_at
Efcbp1
BB392041
0.36


1452135_at
Gpx6
AV001252
0.34


1460623_at
Skap2
BB753881
0.34


1417577_at
Trpc3
NM_019510
0.33


1439566_at
Gprin3
BB245373
0.33


1418639_at
Sftpc
NM_011359
0.33


1457142_at
Efcbp1
BB667731
0.32


1439627_at
Zic1
AV031691
0.31


1454659_at
Dctd
BG069699
0.30


1427300_at
Lhx8
D49658
0.30


1416319_at
Adk
NM_134079
0.29


1438292_x_at
Adk
BB559878
0.28


1451191_at
Crabp2
BC018397
0.26


1419577_at
A530089I17Rik
NM_133999
0.26


1440177_at
9.63E+17
BM899529
0.26


1422586_at
Ecel1
NM_021306
0.25


1433787_at
Nell1
AI841091
0.23


1422541_at
Ptprm
NM_008984
0.22


1434458_at
Fst
BB444134
0.21


1427519_at
Adora2a
BG311385
0.18


1433578_at
Slc10a4
BE824538
0.17


1421365_at
Fst
NM_008046
0.15


1424902_at
Plxdc1
AF378760
0.13


1427038_at
Penk1
M13227
0.13


1428547_at
Nt5e
AV273591
0.13


1460244_at
Upb1
NM_133995
0.11


1440148_at
Gpr6
AI852874
0.08


1460710_at
Adora2a
BB748999
0.08


1418950_at
Drd2
NM_010077
0.03


1435211_at
Ttc12
BB554351
0.00





Fold-change values (FCH) are given relative to D1 (striatonigral) cells.


Probe = Affymetrix probe identification;


Symbol = official gene symbol.




































































































































































































































































































































































TABLE 11



Real time PCR analysis of D1 enriched

messages: range from replicates.







Fold enrichment in D1


Gene
versus D2 cell type





Eya1
19.85-21.51


Tac1 (known)
10.97-11.87


Isl1
10.06-10.87


Gng2
5.91-6.43


Chrm4 (known)
5.02-5.57


Drd1a (known)
2.96-3.4


Crym
1.57-1.88



































TABLE 12



Real time PCR analysis of D2-enriched

messages: range from replicates.







Fold enrichment in D2 versus


Gene
D1 cell type





Drd2 (known)
49.79-51.55


Gpr6
12.30-12.81


Lhx8
3.57-4.06


Gpr88
1.94-2.13


Trpc4
1.93-2.25


Tpm2
1.84-1.96





























4) Large Scale Validation of BACarray Profiling of Striatonigral and Striatopallidan MSNs
Large-scale validation of the data using publicly available gene expression databases was carried out. Data were pooled from D1 and D2 BACarray experiments to represent MSN-enriched subtypes and compared to data collected from the total RNA of one whole brain (minus striatum)
Table 13). The analysis resulted in detection of several thousand translated mRNAs enriched in striatum relative to whole brain, including all previously-known striatal-enriched genes: Ppp1r1b/Darpp-32 (S. I. Walaas, P. Greengard, J Neurosci 4, 84-98 (January, 1984),) Ptpn5/Step (P. J. Lombroso, J. R. Naegele, E. Sharma, M. Lerner, J Neurosci 13, 3064-74 (July, 1993),) Arpp-19 (J. A. Girault, A. Horiuchi, E. L. Gustafson, N. L. Rosen, P. Greengard, J Neurosci 10, 1124-33 (April, 1990),) Arpp-21/RCS (C. C. Ouimet, H. C. Hemmings, Jr., P. Greengard, J Neurosci 9, 865-75 (March, 1989),) Gna1/Go1f (D. Herve et al., J Neurosci 13, 2237-48 (May, 1993),) Rhes/Rasd2 (J. D. Falk et al., J Neurosci Res 57, 782-8 (Sep. 15, 1999),) Rgs9 (S. J. Gold, Y. G. Ni, H. G. Dohlman, E J Nestler, J Neurosci 17, 8024-37 (Oct. 15, 1997),) Adcy5 (C. E. Glatt, S. H. Snyder, Nature 361, 536-8 (Feb. 11, 1993),) Gng7 (J. B. Watson et al., J Neurosci Res 39, 108-16 (Sep. 1, 1994),) Rasgrp2 (H. Kawasaki et al., Proc Natl Acad Sci U S A 95, 13278-83 (Oct. 27, 1998),) Pde1b (J. W. Polli, R. L. Kincaid, Proc Natl Acad Sci USA 89, 11079-83 (Nov. 15, 1992),) Pde10a (K. Fujishige, J. Kotera, K. Omori, Eur J Biochem 266, 1118-27 (December, 1999),) Gpr88 (K. Mizushima et al., Genomics 69, 314-21 (Nov. 1, 2000),) Rarb (W. Krezel, P. Kastner, P. Chambon, Neuroscience 89, 1291-300 (1999),) and Strn4 (F. Castets et al., J Cell Biol 134, 1051-62 (August, 1996)) as well as the transcription factors Foxp1, Foxp2 (R. J. Ferland, T. J. Cherry, P. O. Preware, E. E. Morrisey, C. A. Walsh, J Comp Neurol 460, 266-79 (May 26, 2003),) Ebf1 (S. Magdaleno et al., PLoS Biol 4, e86 (2006)), and Zfp503/Nolz (C. W. Chang et al., Proc Natl Acad Sci USA 101, 2613-8 (Feb. 24, 2004).)
Table 13). As independent confirmation of these data, mRNA expression patterns were examined for a subset of the candidate MSN-expressed genes in the GENSAT/Brain Gene Expression Map (BGEM) and Allen Brain Atlas (ABA) in situ hybridization (ISH) databases (http://www.ncbi.nlm.nih.gov/projects/gensat/; http://www.stjudebgem.org/) Only those genes for which expression data were available in both gene expression atlases were chosen. The data are presented in FIG. 29 . Of the first 100 genes appearing in the MSN-enriched dataset, 26 were present in both the BGEM and ABA public ISH atlases. Enriched striatal expression is evident for 22 of these genes (
Table 13), panel a) ISH data were available in the GENSAT/BGEM and ABA for 16 genes appearing between numbers 1,000 to 1,100 on the MSN enriched list. In this case, striatally-enriched expression is apparent for 7 of these genes ((
Table 13); panel b). Expression analysis in sagittal sections of genes which were amongst the top 100 (a) or 1,000-1,100 (b) genes identified in the study as MSN-enriched, with the rank order of each gene noted below the gene name. Non-redundant gene ranking was calculated using the highest-ranked probeset corresponding to each gene, with redundant probesets eliminated. Left panel, in situ hybridization images taken from the Allen Brain Atlas (Allen Brain Atlas [Internet]. Seattle (Wash.): Allen Institute for Brain Science. © 2006. Available from: http://www.brain-map.org.; E. S. Lein et al., Nature 445, 168-76 (Jan. 11, 2007)); right panel, in situ hybridization images taken from the Brain Gene Expression Map (BGEM) database (http://www.stjudebgem.org/) (S. Magdaleno et al., PLoS Biol 4, e 86 (April, 2006). Allen Brain Atlas images all correspond to adult brain; BGEM images all correspond to adult brain except for the following, for which the oldest available data were postnatal day 7 (P7): Drd2, Ppp1r1b, Dlx6, Gdnf, Bc111b, Foxg1, Limd2, Fem1b, Dyn111, Atbf1, Foxo3a, Dnalc4, Mtmr7, Dnmt3a.





TABLE 13



Genes differentially translated between medium spiny neurons (MSNs)

and the rest of the brain.






Probe
Symbol
GenBank
FCH








1420437_at
Indo
NM_008324
198.43

1427343_at
Rasd2
BC026377
149.45

1457132_at

BF456117
133.95

1458342_at
Tmem90a
BB313069
108.01

1418950_at
Drd2
NM_010077
85.64

1438355_at
Tmem90a
AI414870
79.94

1441368_at

BB102769
71.65

1426814_at
AU024582
BM248309
57.27

1418782_at
Rxrg
NM_009107
56.75

1423171_at
Gpr88
BE947345
52.53

1457143_at
LOC665139
BB322292
50.59

1426438_at
Ddx3y
AA210261
49.33

1419696_at
Cd4
NM_013488
45.51

1458499_at
Pde10a
AW123977
41.50

1427344_s_at
Rasd2
BC026377
34.92

1450213_at
Pde7b
NM_013875
33.08

1455190_at
Gng7
BM114283
31.37

1429285_at
Serpina9
AK009343
29.48

1454906_at
Rarb
BB266455
28.73

1443287_at
Gm1337
BB555669
28.56

1428642_at
Slc35d3
AK018094
28.48

1417680_at
Kcna5
NM_008419
28.33

1451331_at
Ppp1r1b
BC026568
28.25

1442558_at
Ptprn2
BB180191
27.93

1454867_at
Mn1
BB234631
27.26

1437750_at
Tmem158
BI133257
26.54

1457919_at
D030054H15Rik
BB446049
25.08

1427807_at
ENSMUSG00000060559
BC017159
24.97

1436566_at
Rab40b
AV364488
24.65

1456818_at
Stk32a
BB279083
24.52

1452507_at
Dlx6
AF022078
24.32

1457651_x_at
Rem2
BB270375
24.03

1428781_at
Dmkn
BI452905
23.79

1460710_at
Adora2a
BB748999
23.75

1455629_at
Drd1a
BE957273
23.51

1422079_at
Prkch
NM_008856
22.36

1435296_at
Adra2c
AV349563
21.79

1440148_at
Gpr6
AI852874
20.60

1437935_at
4930486G11Rik
BB821151
19.59

1427523_at
Six3
AI509029
19.13

1419389_at
Pde10a
BQ180352
19.09

1454581_at
5330425B07Rik
AK019908
19.03

1440166_x_at
Htr1d
BB378627
18.87

1426815_s_at
AU024582
BM248309
18.68

1419080_at
Gdnf
NM_010275
17.98

1435211_at
Ttc12
BB554351
17.93

1438022_at
Rab11fip3
BQ266518
17.69

1445539_at

BE687857
17.64

1449420_at
Pde1b
NM_008800
17.44

1427683_at
Egr2
X06746
16.79

1456640_at
Sh3rf2
AW910872
16.61

1456280_at
Clspn
BG067086
16.53

1451236_at
Rerg
BC026463
16.46

1460262_a_at
1700037H04Rik
NM_026091
16.39

1460038_at
Pou3f1
BG065255
16.23

1443694_at
Rgs20
BB794177
16.06

1417804_at
Rasgrp2
NM_011242
16.01

1451280_at
Arpp21
BB159263
15.99

1450339_a_at
Bcl11b
NM_021399
15.73

1449977_at
Egr4
NM_020596
15.61

1457424_at
Eya1
BB760085
15.60

1441000_at
EG237749
BB076832
15.52

1454043_a_at
Kcnab1
AK015412
15.48

1438841_s_at
Arg2
AV002218
15.44

1417210_at
Eif2s3y
NM_012011
15.28

1429229_s_at
4930534B04Rik
BE980134
15.28

1454762_at
Nox1
BM932447
15.26

1419390_at
Pde10a
BQ180352
15.22

1435366_at
D430042O09Rik
BB486367
15.19

1425756_at
Rab40b
AF425643
15.18

1418744_s_at
Tesc
NM_021344
14.99

1416627_at
Spint1
NM_016907
14.90

1436650_at
Filip1
AV241894
14.84

1460136_at
AW047481
AI462839
14.68

1435808_at
A230051G13Rik
BB174377
14.53

1421140_a_at
Foxp1
BG962849
14.38

1437698_at
Myo5b
AV370579
14.36

1416456_a_at
Chia
BC011134
14.31

1442166_at
Cpne5
BB273427
14.21

1456051_at
Drd1a
BB282271
13.84

1421365_at
Fst
NM_008046
13.82

1420496_at
F12
NM_021489
13.79

1436092_at

BB336256
13.75

1448327_at
Actn2
NM_033268
13.69

1429228_at
4930534B04Rik
BE980134
13.65

1419826_at
Kif17
AW492270
13.61

1436734_at
E130309F12Rik
BB523550
13.60

1421353_at
Pde7b
NM_013875
13.54

1425833_a_at
Hpca
AF326551
13.53

1454656_at
Spata13
AV271736
13.50

1455298_at
Id4
BB306828
13.39

1425363_at
B4galnt1
BC022180
13.23

1439854_at
Hrk
BQ175572
13.22

1434008_at
Scn4b
BE993937
13.11

1417812_a_at
Lamb3
NM_008484
13.10

1435221_at
Foxp1
BM220880
13.06

1429617_at
Cyld
BM119209
12.96

1418357_at
Foxg1
NM_008241
12.81

1436532_at
Dcamkl3
BB326709
12.78

1441468_at

BB326028
12.77

1447861_x_at
Mrg1
AV329643
12.66

1447766_x_at
Limd2
AV003249
12.63

1454877_at
Sertad4
BQ174721
12.63

1418847_at
Arg2
NM_009705
12.57

1422720_at
Isl1
BQ176915
12.56

1417704_a_at
Arhgap6
NM_009707
12.45

1455361_at
Dgkb
AW493391
12.28

1432073_at
1700113I22Rik
AK007198
12.27

1418743_a_at
Tesc
NM_021344
12.18

1444734_at
A330001L22Rik
BB183877
12.17

1433815_at
Jakmip1
AV290082
12.08

1452217_at
Ahnak
BE570050
12.02

1452135_at
Gpx6
AV001252
11.98

1460325_at
Pum1
BB837171
11.96

1439618_at
Pde10a
AI448308
11.94

1434521_at
Rfxdc2
BB148972
11.92

1435285_at
Mpped2
BB731805
11.92

1416266_at
Pdyn
AF026537
11.84

1422230_s_at
Cyp2a5
NM_007812
11.82

1422706_at
Tmepai
AV370981
11.81

1448468_a_at
Kcnab1
AF033003
11.74

1434917_at
Cobl
BQ173923
11.68

1418881_at
Efcbp2
NM_054095
11.68

1433743_at
Dach1
BG075820
11.67

1441733_s_at
Nup153
C88147
11.66

1442077_at
Htr1b
BB197581
11.66

1433566_at
Rasl10b
BB381618
11.54

1423117_at
Pum1
BB837171
11.50

1426439_at
Ddx3y
AA210261
11.48

1436216_s_at
2610204M08Rik
BM234799
11.46

1431751_a_at
Mpped2
AK012553
11.40

1437865_at
Spata13
AW546433
11.40

1418691_at
Rgs9
NM_011268
11.39

1442058_s_at
Psmc3ip
BE687992
11.38

1419033_at
2610018G03Rik
AW556821
11.36

1455564_at
Bcr
BQ176236
11.36

1455156_at
Strn
BG519214
11.35

1434664_at
2410129H14Rik
BI153133
11.33

1415953_s_at
Mark2
BI686265
11.31

1417129_a_at
Mrg1
U68384
11.26

1436275_at
Kcnip2
AW490636
11.23

1456515_s_at
Tcfl5
AV044715
11.15

1453222_at
4833427B12Rik
BE863648
11.04

1425870_a_at
Kcnip2
AF439339
10.91

1420666_at
Doc2b
BM117900
10.75

1459941_at
Asph
BB096900
10.73

1448913_at
Smarcd1
NM_031842
10.71

1440741_at
Htr1d
BB829587
10.68

1457038_at
Frem2
BM201912
10.66

1458406_at
AI429294
BG144063
10.63

1430686_at
4833418N02Rik
AK014719
10.62

1446877_at
C230014O12Rik
BB381622
10.52

1441928_x_at
Ell
BB139475
10.44

1416350_at
Klf16
NM_078477
10.40

1444978_at

BM223267
10.36

1417356_at
Peg3
AB003040
10.34

1431386_s_at
Mbtps1
AK002809
10.32

1421943_at
Tgfa
M92420
10.27

1435553_at
Pdzd2
AV376136
10.17

1427931_s_at
Pdxk
BG063905
10.16

1427682_a_at
Egr2
X06746
10.09

1444681_at
Erc2
BB749686
10.07

1422153_a_at
Asb11
NM_026853
10.00

1439566_at
Gprin3
BB245373
9.99

1420146_at
Tfb1m
AI429207
9.98

1449957_at
Ptprv
NM_007955
9.95

1426241_a_at
Scmh1
AB030906
9.94

1424208_at
Ptger4
BC011193
9.86

1450026_a_at
B3gnt2
AV306734
9.85

1436738_at
Pif1
AV094878
9.75

1434248_at
Prkch
BM243756
9.75

1422068_at
Pou3f1
NM_011141
9.75

1448374_at
Med28
AK005130
9.72

1456760_at
Centg3
BB342676
9.70

1422813_at
Cacng1
NM_007582
9.69

1449425_at
Wnt2
BC026373
9.64

1438799_at
Dlx6os1
AV338180
9.63

1456283_at
Neto1
AV346211
9.57

1425351_at
Srxn1
BC011325
9.57

1424576_s_at
Cyp2c44
BC025819
9.56

1423544_at
Ptpn5
BB188812
9.53

1433923_at
Krt77
AV230775
9.53

1430640_a_at
Prkar2b
BI695530
9.52

1427779_a_at
Cd4
U75219
9.51

1452966_at
9130430L19Rik
AK020296
9.46

1425130_a_at
Ptpn5
U28216
9.41

1440711_at
C630001G18Rik
BB426320
9.37

1431936_a_at
Neu2
AK009828
9.36

1434025_at
Klf5
BG069607
9.33

1441867_x_at
4930534B04Rik
AI480494
9.30

1427912_at
Cbr3
AK003232
9.27

1436002_at

BB484128
9.23

1449502_at
Dazl
NM_010021
9.21

1430612_at
1810033B17Rik
BB533148
9.19

1455242_at
Foxp1
BM220880
9.18

1441105_at

BE980314
9.16

1447017_at

BE956696
9.03

1435334_at
Ttc7
BG866904
8.97

1436998_at
Ankrd43
BB428991
8.96

1454086_a_at
Lmo2
AK013416
8.87

1419542_at
Dazl
NM_010021
8.82

1442234_at
Chst2
BB770422
8.72

1449331_a_at
Rapsn
NM_009023
8.68

1442021_at
Mppe1
BB502545
8.65

1429201_at
Cyld
AK013508
8.55

1440435_at
Ky
BB126077
8.55

1441350_at
Fgf3
AV302620
8.53

1434519_at
Ddah1
AW556888
8.53

1452298_a_at
Myo5b
AW546331
8.51

1422407_s_at
Hras1
NM_008284
8.45

1416996_at
Tbc1d8
BC005421
8.43

1428873_a_at
4121402D02Rik
AW495537
8.37

1422448_at
Tff2
NM_009363
8.36

1443036_at
Zfp804a
BG073535
8.35

1417522_at
Fbxo32
AF441120
8.32

1453799_at
9430038I01Rik
AK020460
8.31

1438224_at
Zswim5
BE572265
8.28

1430864_at
Ttll9
AK015740
8.25

1451008_at
St8sia3
BB360510
8.25

1453330_at
0610010D24Rik
AK002458
8.24

1420872_at
Gucy1b3
BF472806
8.22

1444383_at
1110014J01Rik
AV347845
8.21

1424132_at
Hras1
BC011083
8.20

1420695_at
Dach1
BB522228
8.13

1438266_at
Adamtsl5
BB764453
8.09

1456054_a_at
Pum1
BB314559
8.08

1422157_a_at
Itgb1bp1
NM_008403
8.06

1420961_a_at
Ivns1abp
NM_028582
8.05

1424899_at
Nmnat3
BC005737
7.97

1424409_at
Cldn23
BC019534
7.94

1435071_at
Zfyve1
AV327165
7.93

1437353_at
Setd1b
BB494182
7.87

1456830_at
Ppp1r2
BB542221
7.87

1445606_a_at
2900009J06Rik
BB663189
7.87

1435227_at

BM117007
7.85

1446455_at

BB520649
7.85

1443676_at
Slc4a4
AV339935
7.83

1429316_at
Rasgef1a
AK018120
7.82

1438417_at
Pwwp2
BB809239
7.82

1434996_at
Slc25a16
AV316233
7.79

1422621_at
Ranbp2
BM507707
7.79

1452176_at
Nup153
BB292874
7.78

1437460_x_at
Rin1
BB264363
7.78

1447642_x_at
Dmwd
AV354897
7.70

1417341_a_at
Ppp1r2
NM_025800
7.69

1431349_at
Hnrpab
AK013709
7.65

1438441_at
Id4
AI323288
7.64

1416242_at
Klhl13
NM_026167
7.64

1441963_at
Ubox5
BB427703
7.64

1422678_at
Dgat2
AK002443
7.60

1447359_at
Zfp575
AI326876
7.58

1450183_a_at
Sh2b3
NM_008507
7.58

1451517_at
Rhobtb2
AF420001
7.55

1437017_at
AI480653
BB046727
7.54

1455296_at
Adcy5
BE952286
7.52

1422779_at
Smpd3
NM_021491
7.49

1434531_at
Mgat5b
BB400317
7.46

1450072_at
Ash11
BG694892
7.45

1422335_at
Adra2c
NM_007418
7.45

1417062_at
Armc10
NM_026034
7.45

1426454_at
Arhgdib
AK002516
7.38

1443073_at
LOC545681
BB355954
7.38

1458407_s_at
AI429294
BG144063
7.36

1453323_at
2900079G21Rik
AK013806
7.34

1438664_at
Prkar2b
BB216074
7.27

1428839_at
Wdr53
AK005105
7.27

1442708_at

BB504418
7.26

1418467_at
Smarcd3
NM_025891
7.25

1455401_at
Camkk2
AW061083
7.24

1434732_x_at
Tomm7
AV044898
7.24

1429618_at
Cyld
BM119209
7.23

1428860_at
4930572J05Rik
AK019809
7.22

1453264_at
Marveld3
AK007346
7.22

1421142_s_at
Foxp1
BG962849
7.22

1418714_at
Dusp8
NM_008748
7.20

1452077_at
Ddx3y
AA210261
7.19

1428370_at
1500011B03Rik
AK018264
7.19

1454832_at
Phactr1
BG228702
7.19

1434153_at
Shb
BI408715
7.18

1456475_s_at
Prkar2b
BB216074
7.15

1422722_at
1700001K19Rik
NM_025488
7.14

1434495_at
Patz1
BM208058
7.14

1423532_at
Rnf44
AI850285
7.12

1437091_at
Accn4
AV323885
7.11

1418017_at
Pum2
BI689507
7.10

1423049_a_at
Tpm1
AK002271
7.10

1455080_at
Ppp1r16b
BB375209
7.10

1416239_at
Ass1
NM_007494
7.10

1428338_at
Spata2L
AK019166
7.07

1425104_at
Kctd1
BC022615
7.06

1433627_at
Sec23ip
AW546839
7.02

1455085_at
1700086L19Rik
BI526033
7.02

1416804_at
Ehbp111
NM_053252
7.00

1452729_at
Dpm3
BE446919
7.00

1419234_at
Helb
BG070273
7.00

1449261_at
Pbx2
NM_017463
6.99

1456336_at
A330102K23Rik
BB182912
6.99

1416983_s_at
Foxo1
AI462296
6.99

1442220_at

BB313387
6.97

1455961_at

AV174022
6.96

1460567_at
Rfxdc2
BB148972
6.94

1437579_at
Nek2
C77054
6.94

1452913_at
Pcp4l1
AV337888
6.93

1441223_at
3-Mar
BB260801
6.93

1435504_at
Clip4
BM217861
6.93

1434077_at
Wdr37
AV222037
6.91

1452879_at
Synpo2
AI848603
6.90

1460285_at
Itga9
NM_133721
6.90

1424796_at
1700054N08Rik
BC024705
6.89

1435125_at
BB120497
BB303627
6.87

1433962_at
6720458F09Rik
BB131965
6.86

1429098_s_at
Nhej1
AK006481
6.85

1452411_at
Lrrc1
BG966295
6.85

1422159_at
Ppef2
BC027049
6.83

1452166_a_at
Krt10
AK014360
6.83

1416981_at
Foxo1
AI462296
6.82

1437092_at
Clip4
AV042829
6.81

1430306_a_at
Atp6v1c2
AK004157
6.80

1421442_at
Flt4
NM_008029
6.77

1435649_at
Nexn
BM225804
6.75

1455683_a_at
Tbc1d8
BB451404
6.73

1434649_at
Ccm2
BI903794
6.72

1455804_x_at
Oxct1
AV213379
6.71

1423344_at
Epor
AK010968
6.69

1460555_at
6330500D04Rik
BM242294
6.68

1436799_at
Rpl23a
BB386177
6.67

1426687_at
Map3k3
BG175594
6.66

1434032_at
Centg3
BQ175381
6.65

1423530_at
Stk32c
BB320288
6.63

1443543_at

BE980616
6.63

1447100_s_at

C80506
6.62

1437842_at
Plcxd1
BB311508
6.59

1420534_at
Gucy1a3
AK004815
6.59

1455301_at
Wipf3
BG064092
6.59

1429637_at
2210419I08Rik
AK008987
6.57

1442051_at
Hist2h3c1
BE691662
6.54

1434458_at
Fst
BB444134
6.54

1435605_at
Actr3b
BB125424
6.53

1422034_a_at
Palm
NM_023128
6.53

1419742_at
1700037H04Rik
NM_026091
6.52

1438171_x_at
Mettl9
BB056666
6.52

1448807_at
Hrh3
NM_133849
6.50

1437097_at
Zbed3
BB703866
6.49

1424759_at
Arrdc4
BC017528
6.49

1438210_at
Gpr149
BB126999
6.48

1441340_at
Cep68
BF018298
6.47

1443887_at
Ankrd13c
BE132758
6.47

1422608_at
Arpp19
BE648432
6.45

1452917_at
Rfc5
AK011489
6.43

1416982_at
Foxo1
AI462296
6.43

1418894_s_at
Pbx2
NM_017463
6.42

1449158_at
Kcnk2
NM_010607
6.42

1448978_at
Ngef
NM_019867
6.41

1427196_at
Wnk4
BG074348
6.41

1424507_at
Rin1
BC011277
6.40

1441089_at
Eif2c1
BB205493
6.40

1427293_a_at
Auts2
AK012475
6.39

1457195_at
Plekhm1
BB206527
6.38

1436930_x_at
Hmbs
BB000512
6.37

1434359_at
6330500D04Rik
AV329070
6.37

1420444_at
Slc22a3
NM_011395
6.37

1417440_at
LOC675933
NM_033566
6.37

1425503_at
Gcnt2
AB037596
6.36

1438784_at
Bcl11b
BB329234
6.34

1460044_at
Onecut2
BB389395
6.34

1439576_at

BB325333
6.34

1428777_at
Spred1
AK017680
6.34

1430413_at
Tmem29
AK018228
6.34

1422293_a_at
Kctd1
NM_134112
6.33

1423488_at
Mmd
BC021914
6.33

1436645_a_at
Cnot4
BB066603
6.32

1445404_at
Kif27
BB054454
6.32

1428547_at
Nt5e
AV273591
6.32

1454752_at
Rbm24
AV307961
6.29

1452092_at
4631426J05Rik
AK019474
6.28

1447830_s_at
Rgs2
BB034265
6.27

1455447_at
D430019H16Rik
BM116882
6.26

1452904_at
1700026L06Rik
BQ175881
6.26

1444363_at

BB269445
6.26

1437839_x_at
Mrpl11
AV209741
6.25

1445385_at

BB551855
6.25

1419247_at
Rgs2
AF215668
6.24

1416886_at
C1d
NM_020558
6.21

1419378_a_at
Fxyd2
NM_052823
6.20

1450366_at
Hrk
NM_007545
6.19

1428495_at
2410003K15Rik
BF300229
6.19

1439844_at
8430426J06Rik
BE628126
6.18

1455345_at
Phf15
BI663145
6.16

1449304_at
2310061J03Rik
NM_133677
6.16

1435564_at
C230078M08Rik
BB547893
6.16

1451819_at
Zswim6
BC021311
6.16

1449141_at
Fblim1
BG070068
6.15

1448860_at
Rem2
NM_080726
6.15

1450835_a_at
Gfra4
NM_020014
6.15

1435096_at
Ric8b
BB667093
6.15

1454901_at
Ypel2
BG069663
6.15

1423270_at
Nedd4l
BB663717
6.14

1452202_at
Pde2a
BG069616
6.12

1442754_at
C030013G03Rik
BE692283
6.10

1449863_a_at
Dlx5
NM_010056
6.09

1422134_at
Fosb
NM_008036
6.09

1430222_at
9130007G19Rik
BB538672
6.09

1434648_a_at
Ccm2
BI903794
6.09

1428139_at
Tmem180
AK015988
6.08

1439947_at
Cyp11a1
C87524
6.08

1421141_a_at
Foxp1
BG962849
6.08

1415976_a_at
Carhsp1
AU080787
6.07

1433988_s_at
C230098O21Rik
BG075755
6.06

1422705_at
Tmepai
AV370981
6.06

1443334_at
D430042O09Rik
BB487579
6.06

1434909_at
Rragd
BF462770
6.04

1456930_at
Camsap1
BE989461
6.03

1416605_at
Nola2
BC024944
6.03

1417090_at
Rcn1
NM_009037
6.02

1460686_at
Cntd1
BC006866
6.02

1430190_at
1700041C02Rik
AK006668
6.02

1430616_at
4930528A17Rik
BB185833
6.01

1419248_at
Rgs2
AF215668
5.99

1417524_at
Cnih2
NM_009920
5.97

1453260_a_at
Ppp2r2a
AK010380
5.95

1443612_at
Tmem16c
AW123199
5.95

1424248_at
Arpp21
BB159263
5.93

1449843_at
St8sia2
BG071333
5.93

1420391_at
Pard3
NM_033620
5.93

1434115_at
Cdh13
BQ176681
5.92

1457052_at
AW536275
BG064867
5.92

1456838_at
BC072620
BE995608
5.92

1436538_at
Ankrd37
AV084342
5.91

1438226_at
AU022252
AV349089
5.90

1422208_a_at
Gnb5
BC016135
5.89

1452368_at
Bcr
AI853148
5.88

1416488_at
Ccng2
U95826
5.87

1435222_at
Foxp1
BM220880
5.87

1432579_at
Rshl2b
AA544511
5.85

1417658_at
Tbrg4
NM_134011
5.85

1422923_at
Fgf3
NM_008007
5.85

1421037_at
Npas2
BG070037
5.85

1431711_a_at
9030409G11Rik
AK018497
5.85

1431242_at
6330575P09Rik
BE995561
5.85

1452911_at
Spred1
AK017680
5.84

1458133_at

BB360008
5.81

1438019_at
Ippk
BM196347
5.81

1417003_at
0610012G03Rik
BC021536
5.80

1448231_at
Fkbp5
U16959
5.80

1428074_at
Tmem158
BE981853
5.80

1456401_at
Cacnb2
BB078175
5.79

1423376_a_at
Dok4
AV341904
5.77

1443365_at
Htr4
BB431008
5.76

1416087_at
Ap1s1
NM_007457
5.76

1429104_at
Limd2
AK012581
5.76

1435218_at
Rasgef1a
BI134758
5.75

1454742_at
Rasgef1b
BB003229
5.75

1437948_x_at
Eif3s6ip
BB443362
5.75

1447640_s_at
Pbx3
BB554874
5.75

1460718_s_at
Mtch1
AF192558
5.74

1453972_x_at
Pparbp
AK005207
5.74

1430520_at
Cpne8
AW548480
5.71

1418015_at
Pum2
BI689507
5.70

1455701_at
Snx26
BI965517
5.67

1436160_at
Krt26
BB150142
5.67

1458025_at
Ttc7
BB519333
5.66

1427005_at
Plk2
BM234765
5.66

1434682_at
Zfp770
AV347367
5.64

1439291_at

BB354792
5.64

1438680_at
Auts2
BB429147
5.63

1443552_at
E230008N13Rik
BM932485
5.63

1453089_at
3110079O15Rik
AA259365
5.62

1440501_at
AU041480
BG072095
5.62

1440940_at
Cacnb1
BE949510
5.62

1446840_at
Thrap1
BQ032894
5.62

1460327_at
Gpr88
BE947345
5.57

1426230_at
Sphk2
AK016616
5.55

1419130_at
Deadc1
NM_025748
5.54

1434967_at
Zswim6
BM938007
5.53

1432782_at
4933435C09Rik
AK017064
5.52

1441967_at
Pddc1
BB467791
5.51

1433468_at
6430527G18Rik
BB770958
5.51

1435741_at
Pde8b
BB312125
5.49

1437400_at
Nedd41
BB309512
5.48

1460206_at
Grasp
NM_019518
5.47

1433767_at
1110018G07Rik
AV257687
5.45

1420918_at
Sgk3
BB768208
5.45

1438189_s_at
Rai16
BB492312
5.44

1433507_a_at
Hmgn2
BE553881
5.42

1450930_at
Hpca
AK002992
5.42

1418373_at
Pgam2
NM_018870
5.40

1427278_at
Clip4
AK017914
5.40

1430013_at
Git1
BE993118
5.39

1437586_at
Cnot4
BB756908
5.38

1439821_at
Lrp2bp
BB075157
5.38

1453268_at
Thg11
AV224102
5.37

1451483_s_at
1700054N08Rik
BC024705
5.35

1436725_at
E130306D19Rik
BM226118
5.34

1425123_at
BC025816
BC025816
5.34

1442434_at
D8Ertd82e
BM195829
5.32

1444379_at
Pwwp2
BB318408
5.31

1416558_at
Melk
NM_010790
5.30

1439855_at
Tmtc1
AV341449
5.30

1427045_at
Synpo
AI849322
5.30

1450480_a_at
Gprk6
AF040749
5.30

1427015_at
C230021P08Rik
BI732921
5.30

1417829_a_at
Rab15
NM_134050
5.29

1457632_s_at
Mrg1
BB207647
5.29

1453040_at
2810402A17Rik
AK013345
5.28

1445459_at
Sstr5
BB023293
5.27

1455670_at

BQ175884
5.27

1424475_at
Camkk2
BI157430
5.26

1424020_at
Arl6ip6
BB837198
5.26

1444522_at

BM942961
5.26

1434124_x_at
2400001E08Rik
AV100071
5.26

1436100_at
Sh2d5
AV347995
5.25

1437453_s_at
Pcsk9
AV010795
5.24

1431665_a_at
Timm8b
AK004190
5.23

1420752_at
Dtx3
NM_030714
5.23

1453165_at
Mettl4
BB701076
5.22

1454256_s_at
1700113I22Rik
AK004415
5.22

1427954_at
BC048403
BC028785
5.21

1450227_at
Ankrd6
BM199504
5.21

1454309_at
2810002N01Rik
BB646622
5.20

1435941_at
Rhbdl3
BM118307
5.20

1450723_at
Isl1
BQ176915
5.19

1419006_s_at
Peli2
NM_033602
5.18

1434301_at
D330050I23Rik
BE303700
5.18

1423442_a_at
Fbxw2
BE854125
5.18

1434828_at
B430201A12Rik
BM200103
5.18

1431717_at
3526401B18Rik
AK014386
5.18

1439028_at
Ufm1
AI415161
5.17

1444615_x_at
Runx1t1
AV327778
5.17

1434775_at
Pard3
AW543460
5.17

1437980_at
9130230N09Rik
BB814947
5.16

1455923_at
Kctd8
BB352927
5.16

1455200_at
Pak6
BB818370
5.15

1416479_a_at
Tmem14c
NM_025387
5.15

1452964_at
Ttll11
AK016577
5.15

1422440_at
Cdk4
NM_009870
5.15

1420694_a_at
Dach1
BB522228
5.15

1456197_x_at

BB342782
5.14

1418592_at
Dnaja4
NM_021422
5.14

1421234_at
Tcf1
NM_009327
5.13

1425753_a_at
Ung
BC004037
5.13

1426964_at
3110003A17Rik
AK013984
5.13

1420778_at
Tas1r3
NM_031872
5.12

1433610_at
AA986860
BB522283
5.12

1422807_at
Arf5
NM_007480
5.11

1447559_at
AI834762
AI834762
5.10

1434014_at
Atg4c
BB291836
5.10

1433642_at
Arl15
BB384173
5.09

1426573_at
Me2
BM235734
5.08

1422165_at
Pou3f4
X66603
5.08

1431186_at
Dlg5
BF140264
5.08

1429422_at
4933412E12Rik
AK016788
5.08

1453309_at
9330179D12Rik
BB749938
5.08

1457512_at

BB167650
5.08

1435615_at
Zfp365
BB277790
5.08

1437378_x_at
Scarb1
BB224405
5.07

1429124_s_at
Rexo1
BQ178367
5.07

1455153_at
Zfp236
BQ177220
5.07

1430617_at
Oip5
BB238604
5.06

1427519_at
Adora2a
BG311385
5.06

1436142_at
3526401B18Rik
BM218877
5.06

1438068_at

BB251859
5.04

1429678_at
5730508B09Rik
AK017758
5.04

1455022_at
Strn
BG070684
5.03

1448684_at
Ppp1r2
NM_025800
5.03

1423639_at
Hrh2
AK020259
5.02

1451998_at
Tasp1
BC024597
5.02

1460166_at
Rit2
BB271919
5.02

1451604_a_at
Acvrl1
BC014291
5.02

1450418_a_at
Yipf4
NM_026417
5.01

1453000_at
Camsap1l1
AK005444
5.01

1438665_at
Smpd3
BF456582
5.01

1432184_a_at
2610204M08Rik
AK011884
5.01

1428095_a_at
Tmem24
AK004920
5.01

1427745_x_at
Prpmp5
X63005
5.01

1423802_at
Camkv
BC017634
5.00

1417897_at
Brms1
NM_134155
5.00

1458967_at

BM939341
5.00

1420950_at
Znrf1
BB026596
4.99

1418783_at
Trpm5
AF228681
4.99

1438613_at
Kcna4
BB131475
4.98

1433996_at
Suv39h2
BB296443
4.97

1435423_x_at
4933433P14Rik
AV281088
4.96

1417082_at
Anp32b
NM_130889
4.96

1441883_at
0610010O12Rik
AV092359
4.95

1456225_x_at
Trib3
BB508622
4.95

1434154_at
Kctd13
BQ177107
4.95

1423489_at
Mmd
BC021914
4.94

1452476_at
Cacnb2
W41214
4.94

1447844_at

AV077281
4.93

1433668_at
Pnrc1
BI410130
4.93

1416398_at
Mesdc1
BB474887
4.93

1423260_at
Id4
BB121406
4.92

1440108_at
Foxp2
BM964154
4.92

1430439_at
Mctp1
AK013379
4.91

1440426_at
Nfatc2
BB427970
4.91

1437181_at
Peli2
BM121149
4.89

1422474_at
Pde4b
BM246564
4.89

1455033_at
B430201A12Rik
BB325849
4.88

1430519_a_at
Cnot7
AK007767
4.88

1433451_at
Cdk5r1
BB177836
4.88

1423202_a_at
Ncor1
U22016
4.88

1455754_at
Lmo3
BQ043839
4.87

1425400_a_at
Cited4
BC025116
4.87

1422737_at
Ncoa3
NM_008679
4.87

1442363_at
1110012J17Rik
BB461471
4.86

1428698_at
Dpp8
BM939621
4.86

1450071_at
Ash11
BG694892
4.86

1447711_x_at
4933412E12Rik
BB265147
4.85

1450975_at
Cacng4
BB333636
4.84

1451898_a_at
Sema6c
AF363972
4.84

1426644_at
Tbc1d20
BC002196
4.83

1428872_at
4121402D02Rik
AW495537
4.82

1417393_a_at
C1qdc2
NM_026125
4.82

1453314_x_at
2610039C10Rik
AK012533
4.80

1450700_at
Cdc42ep3
BB012489
4.80

1455178_at
Rutbc1
BB496468
4.80

1424611_x_at
Trub2
BG064045
4.79

1435083_at
Ctxn1
BI155559
4.78

1439422_a_at
C1qdc2
AV048291
4.78

1437707_at
Slmo1
BB835597
4.77

1452533_at
Ryr3
X83934
4.77

1455114_at
Ccnu
BB547482
4.77

1439031_at
Jph4
BB376821
4.76

1451696_at
Zfp64
BC004695
4.76

1424201_a_at
Seh11
AW537349
4.76

1436733_at
E130309F12Rik
BB523550
4.75

1449241_at
Klhl1
NM_053105
4.75

1435435_at
Cttnbp2
BB357580
4.74

1451867_x_at
Arhgap6
AF177664
4.73

1453551_at
Polq
AK020790
4.73

1436563_at
4932441J04Rik
AV277283
4.73

1417530_a_at
Srp9
BI661964
4.72

1428357_at
2610019F03Rik
AK011462
4.72

1424117_at
BC056474
BC024937
4.72

1452063_at
2410081M15Rik
AK010736
4.72

1430729_at
Myg1
AK017955
4.71

1435763_at
Tbc1d16
BI218684
4.71

1417893_at
Sfxn3
NM_053197
4.70

1460610_at
9430057O19Rik
BG143491
4.70

1420094_at
Hnrpdl
AU015266
4.69

1426676_s_at
Tomm70a
BB225670
4.69

1431520_at
4933406J09Rik
AK016696
4.69

1425080_at
Zfp286
BE651907
4.68

1428279_a_at
Atxn7l4
AK013145
4.68

1453781_at

AK009527
4.68

1436013_at
Gsg11
AI852434
4.67

1441879_x_at
Mkrn1
AV218897
4.67

1454572_at
2810414N06Rik
AK013093
4.67

1435585_at
Tceal7
BB378019
4.67

1441051_at
Trim27
BB401251
4.67

1420580_at
4930429B21Rik
NM_026249
4.66

1420446_at
Odf3
NM_027019
4.66

1450306_at
Zp1
NM_009580
4.66

1437069_at
Osbpl8
BG969333
4.66

1423835_at
Zfp503
BB447914
4.66

1416397_at
Mesdc1
BB474887
4.65

1425154_a_at
Csf1
M21149
4.65

1426893_at
C230093N12Rik
BC023470
4.64

1426032_at
Nfatc2
AF289078
4.63

1453577_at
2610018I03Rik
BB399635
4.63

1439888_at

BB271581
4.63

1436421_s_at
Arpc51
BB403233
4.63

1432312_a_at
4931440B09Rik
AK016509
4.62

1423831_at
Prkag2
BB756794
4.62

1439246_x_at
Tnrc6a
BB822587
4.62

1420533_at
Gucy1a3
AK004815
4.61

1418169_at
Zcchc14
BB223737
4.61

1443542_at

BB767151
4.60

1428676_at
Tmprss6
AK004939
4.60

1437146_x_at
Coro7
AV025980
4.60

1428535_at
9430020K01Rik
AK004276
4.59

1418173_at
Krt25
NM_133730
4.59

1443888_at
AU023762
BB426608
4.59

1438327_at
Zfp533
BE981788
4.59

1433937_at
Trp53bp2
BB814564
4.58

1454958_at
Gsk3b
BQ175580
4.58

1420667_at
Doc2b
BM117900
4.58

1448922_at
Dusp19
NM_024438
4.57

1453008_at
2300002D11Rik
AK009004
4.57

1418375_at
Mbd6
NM_033072
4.56

1417443_at
Acot11
NM_025590
4.56

1425328_at
BC008163
AU067643
4.56

1456601_x_at
Fxyd2
AV002675
4.54

1454845_x_at
Mchr1
AW049955
4.54

1425327_at
BC008163
AU067643
4.54

1455772_at
Pgr
BB428874
4.54

1450649_at
Gng10
NM_025277
4.54

1435966_x_at
Mrpl13
BE953095
4.54

1457088_at
Pldn
BB780781
4.54

1432541_at
4930408O17Rik
AK015114
4.53

1422642_at
Cdc42ep3
BB012489
4.53

1416606_s_at
Nola2
BC024944
4.53

1423438_at
Kptn
AK009746
4.53

1420871_at
Gucy1b3
BF472806
4.52

1417372_a_at
Peli1
BC016515
4.52

1455170_at
2810001G20Rik
BB250227
4.51

1438889_at

BB183456
4.51

1460058_at

BE648595
4.51

1427674_a_at
Sez6
D29763
4.51

1440204_at
Foxg1
AW494150
4.50

1423699_at
Ncaph2
BC003900
4.50

1443372_at

BB417900
4.50

1456858_at
Gpr149
BB075339
4.49

1445605_s_at
4921533L14Rik
AI835491
4.49

1441048_at
5930430L01Rik
BB037822
4.49

1460343_at
Neurl
AF401228
4.49

1421364_at
Lrfn1
NM_030562
4.49

1435666_at
Mast3
AW553439
4.48

1434295_at
Rasgrp1
BE691356
4.48

1442562_at

BB346556
4.48

1418016_at
Pum2
BI689507
4.48

1426585_s_at
Mapk1
BM209765
4.47

1426450_at
Plcl2
BM207017
4.47

1425558_at
Klc3
BC017147
4.47

1419838_s_at
Plk4
AI385771
4.46

1417178_at
Gipc2
NM_016867
4.46

1454069_at
9030409C19Rik
AK018495
4.46

1438955_x_at
Ppif
AV209130
4.46

1458399_at
Lrrc3
AI413999
4.45

1448553_at
Myh7
NM_080728
4.45

1453782_at
3021401C12Rik
BI737125
4.45

1439552_at
Trio
BE985618
4.44

1450748_at
Smpd3
NM_021491
4.44

1422799_at
Bat2
AK019427
4.44

1436827_at
Gm944
BB053232
4.44

1440359_at
1700012H17Rik
BB427277
4.44

1427138_at
0610010D24Rik
AW556861
4.44

1454772_at
Ascc3l1
AI327392
4.43

1435624_at
9030409G11Rik
AV345336
4.42

1423186_at
Tiam2
BM228957
4.42

1455548_at
Dlgap4
BB314358
4.41

1423973_a_at
Arf3
BC024935
4.41

1421979_at
Phex
U73910
4.40

1452609_at
Cox4nb
BI133691
4.40

1419757_at
Pitpnm2
NM_011256
4.40

1455024_at
Tlk1
AV269836
4.40

1439548_at
Rap2b
BB390705
4.40

1460244_at
Upb1
NM_133995
4.40

1435319_at
Ihpk2
BB777667
4.39

1451630_at
Ttl
BC018513
4.39

1440901_at
Dgkb
BB279524
4.38

1423563_at
Prrt1
AA739053
4.38

1426097_a_at
Ccdc106
BC018462
4.38

1456755_at
Trak1
AI467545
4.38

1423641_s_at
Cnot7
BC006021
4.38

1424439_at
1810065E05Rik
BC026941
4.38

1429557_at
Mcm8
AK010365
4.37

1415999_at
Hey1
NM_010423
4.37

1439632_at

BQ174397
4.37

1457149_at
Ttc22
BB005679
4.36

1423836_at
Zfp503
BB447914
4.36

1448378_at
Fscn1
NM_007984
4.35

1421897_at
Elk1
AI385733
4.35

1437001_at
Gsk3b
BQ173949
4.35

1435441_at
Ablim2
BB336053
4.34

1449110_at
Rhob
BC018275
4.34

1429122_a_at
1700040I03Rik
AK006658
4.34

1450623_at
Gnb2
NM_010312
4.33

1427607_at
Cacna1h
AB041801
4.33

1419924_at
Fnip1
AW557298
4.33

1426978_at
Klhl2
AW682368
4.32

1421193_a_at
Pbx3
NM_016768
4.32

1438722_at
2610014I16Rik
AI854368
4.31

1420834_at
Vamp2
BG871810
4.31

1425680_a_at
Btrc
AF110396
4.31

1427930_at
Pdxk
BG063905
4.31

1418161_at
Jph3
NM_020605
4.30

1450863_a_at
Dcamkl1
BQ174703
4.30

1439957_at
Gnal
BF457720
4.29

1447530_at
F8a
BB274927
4.29

1421260_a_at
Srm
NM_009272
4.29

1441783_at
Ccni
BB749856
4.28

1428812_at
1700040L02Rik
AK006661
4.28

1452019_at
Cyyr1
AF442733
4.28

1454939_at
Phf20l1
BB268102
4.28

1420427_a_at
Dhx32
NM_133941
4.27

1455632_at
Gnb5
BE446953
4.27

1448718_at
2400001E08Rik
NM_025605
4.26

1432813_at
2900064F13Rik
AK013749
4.26

1430135_at
Dnase2a
AK018651
4.26

1435845_at

BB764479
4.26

1416508_at
Med28
AK005130
4.26

1421199_at
Dlg2
BB622767
4.26

1420887_a_at
Bcl2l1
NM_009743
4.25

1439133_at
4930524O07Rik
AV267216
4.25

1417216_at
Pim2
NM_138606
4.25

1424037_at
Itpka
BC027291
4.25

1422683_at
Irak1bp1
NM_022986
4.24

1454902_at
Prkcz
BB430502
4.24

1417233_at
Chchd4
NM_133928
4.24

1418181_at
pPtp4a3
AK014601
4.24

1424384_a_at
Znrf1
BC006765
4.24

1437306_at
C130092O11Rik
BG071013
4.24

1417890_at
Pdxp
NM_020271
4.24

1434943_at
BC023055
BF464669
4.24

1430358_at
Becn1
AV338072
4.23

1438211_s_at
Dbp
BB550183
4.23

1437780_at
Fancb
AV222467
4.23

1455865_at
Insm1
BB468410
4.22

1456377_x_at
LOC632329
AV010467
4.22

1418893_at
Pbx2
NM_017463
4.22

1453324_at
Nip7
BF459399
4.21

1449063_at
Sec22b
BC009024
4.21

1428951_at
Nol8
AK017551
4.21

1422627_a_at
Mkks
BF581250
4.21

1433816_at
Mcart1
BQ031264
4.21

1439508_at
Rab11b
BI663913
4.21

1433955_at
Brwd1
BB467195
4.20

1436128_at
Plekha8
AI324154
4.20

1458457_at

BB750877
4.20

1448150_at
Nup62
NM_053074
4.20

1460395_at
Nudcd3
BB139222
4.19

1434830_at
Mxd1
AV228517
4.19

1451884_a_at
Lsm2
AF204156
4.19

1447696_x_at
Adcy5
BB091427
4.18

1452984_at
Ccny
AK014507
4.18

1451020_at
Gsk3b
BB831420
4.18

1448877_at
Dlx2
NM_010054
4.17

1455365_at
Cdh8
BB426483
4.17

1448498_at
Rps6ka4
NM_019924
4.17

1427261_at
Wwc1
BQ176786
4.17

1423330_at
Ensa
AK006149
4.17

1453278_a_at
Clip4
AV273445
4.16

1432103_a_at
Sh3gl3
AK012114
4.16

1455102_at
D330037H05Rik
BB213860
4.16

1451917_a_at
Dcamkl1
AF155820
4.16

1433657_at
A130092J06Rik
BI111848
4.15

1442927_at
Ptk2b
BB548690
4.15

1430037_at
Snx27
AK017836
4.15

1449552_at
Zfr
NM_011767
4.15

1435367_at
Mapk4
BQ177154
4.14

1458711_at

BB762344
4.14

1428214_at
Tomm7
BB609428
4.14

1427673_a_at
Sema3e
Z93948
4.14

1427450_x_at
Myo1b
BI080370
4.14

1456290_x_at
Ccm2
BB813044
4.13

1432574_at
4930548F15Rik
BF466430
4.13

1423897_at
Rnf187
AW488376
4.13

1450570_a_at
Cd19
NM_009844
4.13

1422977_at
Gp1bb
NM_010327
4.12

1454844_at
Mchr1
AW049955
4.12

1423785_at
Egln1
BE995700
4.11

1423012_at
Syt7
NM_018801
4.11

1457116_at
Rexo1
BI555624
4.10

1416391_at
Ptcd1
NM_133735
4.10

1456471_x_at
Phgdh
BB204486
4.10

1450142_a_at
Card14
NM_130886
4.09

1442113_at
5330417C22Rik
BB183350
4.09

1439986_at
Dgki
BE647270
4.09

1448709_at
LOC669477
NM_033566
4.09

1423640_at
Synpr
BC026512
4.08

1458443_at
Crtc1
BE986758
4.08

1441894_s_at
Grasp
BB071890
4.08

1426065_a_at
Trib3
BC012955
4.08

1417326_a_at
Anapc11
NM_025389
4.08

1434569_at
AA474455
BB428484
4.08

1460392_a_at
Eny2
BB610210
4.07

1426354_at
Bap1
AK009033
4.07

1457704_at
Zfp533
BE982894
4.07

1458660_at
AI414330
AI414330
4.06

1454721_at
1110018G07Rik
AV257687
4.06

1434403_at
Spred2
AV229054
4.06

1455271_at
LOC620695
BB560177
4.05

1420853_at
Sdc3
BB528350
4.05

1431394_a_at
Lrrk2
AK014938
4.05

1459983_at
Impa2
AI115397
4.05

1455620_at
4930551E15Rik
BQ177219
4.04

1454943_a_at
Paxip1
BM935413
4.04

1434964_at
X83328
BM952039
4.04

1438662_at
Ajap1
AW123234
4.03

1452974_at
Nol8
AK017551
4.03

1435169_at
A930001N09Rik
BB431047
4.03

1425271_at
Psmc3ip
AB000121
4.03

1437642_at
Hrbl
BB243375
4.03

1419137_at
Shank3
NM_021423
4.03

1423673_at
Ldoc11
BC023053
4.03

1448360_s_at
Angel2
NM_021421
4.03

1424523_at
Elmo1
BC024727
4.02

1455206_at
C130006E23
BQ175276
4.02

1422527_at
H2-DMa
NM_010386
4.02

1450928_at
Id4
BB121406
4.02

1458351_s_at
Klhl2
BB428573
4.01

1422902_s_at
Mgea5
NM_023799
4.01

1435942_at
Kcnq2
BE993301
4.01

1454960_at
Smad3
BI646741
4.00

1433854_at
Tmem164
BG064061
4.00

1445653_at
BC031361
BB825492
4.00

1442695_at
C030007I01Rik
AW494399
4.00

1417192_at
Tomm70a
NM_138599
4.00

1447301_at
Akap5
BG228875
4.00

1426416_a_at
Yipf4
AV216410
4.00

1438381_x_at
Ddx47
BB305306
4.00

1425354_a_at
Aggf1
BC027286
4.00

1420192_at
D16Bwg1494e
N28171
4.00

1458074_at
EG328280
BB179240
3.99

1421355_at
Tgm3
NM_009374
3.99

1426031_a_at
Nfatc2
AF289078
3.99

1446498_at
Il20ra
BB551879
3.99

1434920_a_at
Evl
AW553781
3.99

1452531_at
Runx1
X97306
3.99

1452541_at
Epb4.1l2
AJ245854
3.98

1417375_at
Tuba4a
AW491660
3.98

1439932_at
3110003A22Rik
BQ174624
3.98

1459299_at
Myo3b
AV381193
3.98

1420590_at
Has1
NM_008215
3.98

1448990_a_at
Myo1b
AI255256
3.98

1454092_a_at
Gtf2h3
AK017176
3.97

1435638_at
9130221H12Rik
BG808297
3.97

1426580_at
Plk4
BB706079
3.97

1423174_a_at
Pard6b
BE953582
3.97

1450655_at
Pten
AA214868
3.97

1460214_at
Pcp4
NM_008791
3.97

1452281_at
Sos2
Z11664
3.97

1429393_at
Wdr40a
BI558553
3.97

1422897_at
Slc22a12
NM_009203
3.96

1416125_at
Fkbp5
U16959
3.95

1440213_a_at
2010001M06Rik
BE200030
3.95

1420767_at
1700019G17Rik
NM_029331
3.95

1423175_s_at
Pard6b
BE953582
3.95

1435674_at
Rhobtb2
BB351953
3.94

1434736_at
Hlf
BB744589
3.94

1460205_at
Dcakd
NM_026551
3.94

1422255_at
Kcna4
NM_021275
3.94

1433777_at
L3mbtl2
BB152370
3.94

1439659_at
Usp49
BB014981
3.94

1427161_at
Cenpf
BE848253
3.94

1448141_at
1110014J01Rik
NM_029101
3.94

1418657_at
Znhit4
BC019383
3.93

1425006_a_at
Vrk1
BC016676
3.93

1436408_at
Rprml
BE946298
3.93

1453113_at
Wdsub1
BB820363
3.93

1415904_at
Lpl
BC003305
3.92

1426266_s_at
Zbtb8os
BB417508
3.92

1433860_at
6030458C11Rik
BB126127
3.92

1427409_at
8-Mar
BB366341
3.92

1436280_at
Zfp31
BB757838
3.92

1450853_at
Tle4
AU045006
3.91

1460436_at
Ndst1
BI652065
3.91

1424434_at
BC024814
BC024814
3.91

1435043_at
Plcb1
BB794831
3.90

1458413_at
Fbxw8
BB357976
3.90

1454675_at
Thra
BI076689
3.90

1434795_at
Disp1
BB174877
3.90

1445854_at
C230004F18Rik
BB380166
3.90

1420985_at
Ash11
BG694892
3.90

1426383_at
Cry2
BF303057
3.90

1448467_a_at
Ehbp1l1
NM_053252
3.90

1437520_a_at
Nup85
BB320388
3.89

1421477_at
Cplx2
NM_009946
3.89

1417331_a_at
Arl6
NM_019665
3.88

1460738_at
Limd2
AK012581
3.88

1417456_at
Gnpat
NM_010322
3.88

1454789_x_at
Prpf6
BB085604
3.88

1452628_at
Bag5
AK005534
3.87

1417075_at
2010309E21Rik
NM_025591
3.87

1443225_at
Acvr1c
BB396526
3.87

1438309_at

BB432539
3.87

1416744_at
Uap1
NM_133806
3.87

1419166_at
Slc5a2
BC022226
3.87

1436795_at
9630058J23Rik
BM247060
3.86

1448364_at
Ccng2
U95826
3.86

1427358_a_at
Dapk1
BC026671
3.85

1440505_at
Plcxd1
BB187908
3.85

1433847_at
D330017J20Rik
BB098407
3.85

1417114_at
Gmcl1
AF163665
3.85

1419352_at
l7Rn6
BC003916
3.84

1428443_a_at
Rap1gap
AK005063
3.84

1428723_at
2310047M10Rik
AK009886
3.84

1426887_at
Nudt11
BQ174833
3.84

1422439_a_at
Cdk4
NM_009870
3.84

1442039_at
Tox
BF020502
3.84

1419669_at
Prtn3
U97073
3.83

1456970_at
Cdh7
BF472513
3.83

1452295_at
Tmepai
AV291712
3.82

1448511_at
Ptprcap
NM_016933
3.82

1431646_a_at
Stx6
AK009690
3.82

1448414_at
Rad1
NM_011232
3.82

1417355_at
Peg3
AB003040
3.82

1456441_at
Rbm4b
BB667164
3.81

1417214_at
Rab27b
BB121269
3.81

1448554_s_at
Myh7
NM_080728
3.81

1453266_at
Zbtb4
BB767069
3.81

1419611_at
4632415L05Rik
BC023403
3.81

1448919_at
Cd302
NM_025422
3.80

1429670_a_at
Lrriq2
BB321286
3.80

1421975_a_at
Add2
AF189769
3.79

1459054_at
BC035954
BB496939
3.79

1458339_at
Cdadc1
BB231078
3.79

1427038_at
Penk1
M13227
3.79

1417844_at
Med4
NM_026119
3.79

1434306_at
Rab3ip
BF319015
3.78

1439764_s_at
Igf2bp2
BB098431
3.78

1435431_at
2310047M15Rik
AW558989
3.78

1420397_a_at
Spen
NM_019763
3.78

1433558_at
Dab2ip
BM231046
3.78

1420052_x_at
Psmb1
C81484
3.78

1439882_at
Sec23ip
BE685845
3.78

1443952_at
Thra
BI525006
3.77

1432378_at
C030004G16Rik
AK021043
3.77

1457897_at
Iqce
AV245518
3.77

1460671_at
Gpx1
BI219063
3.77

1431771_a_at
Irak1bp1
AK014712
3.77

1422609_at
Arpp19
BE648432
3.77

1427522_at
Arhgap20
BB623455
3.76

1426452_a_at
Rab30
BG070713
3.76

1452932_at
Zbtb7a
AK010379
3.76

1441495_at
Kctd8
BB183090
3.75

1419225_at
Cacna2d3
NM_009785
3.75

1434141_at
Gucy1a3
BG072799
3.75

1450662_at
Tesk1
NM_011571
3.75

1423217_a_at
2510049I19Rik
AV109006
3.75

1434277_a_at
Ypel2
BG069663
3.75

1430630_at

BB236218
3.74

1436986_at
Sntb2
BB219478
3.74

1437006_x_at
Becn1
C86082
3.74

1416603_at
Rpl22
NM_009079
3.74

1435525_at
Kctd17
BI408602
3.74

1437131_x_at
Mrpl11
AV045020
3.74

1453428_at
2700045P11Rik
AK012380
3.74

1444808_at
Pawr
BG070555
3.73

1453083_at
6430701C03Rik
AK018294
3.73

1435430_at
Tmem1
BM200437
3.73

1457310_x_at
Scrt2
BG807042
3.73

1434082_at
Pctk2
BM243464
3.73

1425920_at
Cuedc1
BC025434
3.73

1456106_x_at
Sdccag3
BB343189
3.73

1434805_at
Mllt1
BG063049
3.73

1422457_s_at
Sumo3
NM_019929
3.72

1416113_at
Fkbp8
NM_010223
3.72

1417462_at
Cap1
NM_007598
3.72

1435825_at
Acvrl1
BG969012
3.72

1437703_at
EG382156
BM229128
3.71

1428222_at
Dcamkl2
AK018179
3.71

1451746_a_at
Atg12
AK016474
3.71

1419224_at
Cecr6
NM_033567
3.70

1457341_at

BE991676
3.70

1428914_at
2310014D11Rik
AK009333
3.70

1418081_at
Wbscr18
NM_025362
3.70

1426521_at
D230025D16Rik
BG065702
3.70

1436887_x_at
Grwd1
BB251524
3.70

1445970_at
Ablim2
BG068300
3.70

1438213_at
A830018L16Rik
BB128962
3.70

1429591_at
Tacc1
BE862546
3.70

1440407_at

BE956823
3.69

1426367_at
Cab39l
AK013205
3.69

1436787_x_at
Sec14l3
AV024133
3.69

1448959_at
Ndufs4
NM_010887
3.69

1418789_at
Sntg2
NM_133742
3.69

1447157_at
4930480K23Rik
BB751612
3.68

1444107_at
9430029N19Rik
BB091357
3.68

1454815_at
Sertad2
BI219769
3.68

1434663_at
2410129H14Rik
BI153133
3.68

1434997_at
Cdc2l6
BB510904
3.68

1455820_x_at
Scarb1
BB138434
3.68

1458027_at
Mrpl17
BB343967
3.68

1422787_at
Fkbpl
NM_019873
3.68

1455562_at
Sox12
BF468186
3.68

1421000_at
Cnot4
AI448404
3.68

1422495_a_at
Hmgn1
NM_008251
3.67

1424731_at
Nle1
BC018399
3.67

1451723_at
Cnot6l
BC018506
3.67

1449353_at
Zmat3
NM_009517
3.67

1434825_at
Zfp469
BQ175287
3.67

1451291_at
Obfc2b
BC026942
3.67

1422121_at
Oprd1
L06322
3.67

1423116_at
Dom3z
BG071924
3.67

1429174_at
Wdr34
AK014304
3.67

1427286_at
D11Bwg0517e
BB130195
3.67

1443250_at
Rgs2
AV246932
3.66

1440368_at
Jmjd2b
BG075393
3.66

1435204_at
Prmt8
BG065273
3.66

1435270_x_at
N6amt2
BF730076
3.66

1427300_at
Lhx8
D49658
3.66

1452506_a_at
Ilkap
BC011427
3.66

1428582_at
2010208K18Rik
AK008476
3.66

1430735_at
4930424G05Rik
AK015195
3.66

1433907_at
Pknox2
BQ175824
3.66

1453485_s_at
1110005A03Rik
AK016897
3.65

1437989_at
Pde8b
BB357157
3.65

1448687_at
C1qdc2
NM_026125
3.65

1455703_at
Akt2
BG074736
3.65

1426009_a_at
Pip5k1b
BC003763
3.65

1426701_at
4632419K20Rik
BM055600
3.65

1440823_x_at
D130058I21Rik
BB005209
3.65

1435544_at
Exosc6
BB446614
3.65

1455313_at
Ablim2
BB336053
3.64

1419216_at
Azi1
NM_009734
3.64

1453025_at
2900006F19Rik
AK011824
3.64

1423583_at
Fem1a
AK005041
3.64

1426328_a_at
Scn3b
AY049036
3.64

1422594_at
5730470L24Rik
BC004090
3.64

1426457_at
Slmap
BB473571
3.64

1437392_at
AW123113
AW123113
3.64

1424851_at
Chchd5
BE991735
3.63

1445202_at

BB526676
3.63

1434653_at
Ptk2b
AV026976
3.63

1457246_at
Rai16
AU040322
3.63

1457864_at
Rab11fip3
BB206151
3.63

1419443_at
Sap18
NM_009119
3.63

1415761_at
Mrpl52
AV021593
3.63

1422710_a_at
Cacna1h
NM_021415
3.63

1424082_at
Tbc1d13
BC025586
3.63

1435128_at
Baiap2
BB429313
3.63

1452861_at
2010300C02Rik
AK008485
3.62

1449128_at
Ccdc43
NM_025918
3.62

1436810_x_at
2900010M23Rik
AV046927
3.62

1460261_at
Dlg4
AI646416
3.62

1416533_at
Egln2
NM_053208
3.62

1433151_at
4933415B22Rik
AK016815
3.62

1435921_at
D8Ertd587e
BM223036
3.61

1421107_at
Stk4
NM_021420
3.61

1439569_at
Gpr83
AV291031
3.61

1449072_a_at
N6amt2
NM_026526
3.61

1439731_at
E130309F12Rik
BB546288
3.61

1424777_at
Wdr21
BC003284
3.61

1438128_at
Gnat2
BB427907
3.61

1426894_s_at
C230093N12Rik
BC023470
3.61

1421739_a_at
Matk
NM_010768
3.61

1436639_at
Bola2
BE951004
3.60

1420059_at
2410166I05Rik
AI480750
3.60

1457702_at
Gpr12
BB284435
3.60

1451665_a_at
Ap4s1
BC001985
3.60

1431056_a_at
Lpl
AK017272
3.60

1455064_at
Rab36
BB313586
3.60

1455958_s_at
Pptc7
AI881989
3.60

1438751_at
Slc30a10
BB736474
3.60

1429392_at
Wdr40a
BI558553
3.60

1434216_a_at
Nudt19
BG070689
3.59

1439645_at
Adra2b
BQ031032
3.58

1439557_s_at
Ldb2
BB097063
3.58

1429197_s_at
Rabgap11
BB431654
3.58

1454219_at
Dnajc2
AK013295
3.58

1434704_at
Srpk2
BB529352
3.58

1416783_at
Tac1
NM_009311
3.58

1429752_x_at
Clip4
AV273445
3.58

1422713_a_at
Ube2i
U31934
3.58

1415762_x_at
Mrpl52
AV021593
3.58

1433471_at
Tcf7
AI323642
3.58

1416990_at
Rxrb
BC019432
3.57

1437559_at
Rgs7bp
BB130891
3.57

1423522_at
Npm3
BB811478
3.57

1452452_at

M73818
3.57

1435265_at

BF466929
3.57

1419285_s_at
Cyhr1
NM_019396
3.57

1416285_at
Ndufc1
NM_025523
3.57

1452789_at
Snn
AK012171
3.57

1425211_at
2010003O18Rik
BC021775
3.57

1454720_at
Hs3st5
AV328620
3.57

1428892_at
Ppil1
AK004331
3.57

1439662_at
Homer1
BM124609
3.57

1435233_at
Ncoa2
BM234716
3.56

1423916_s_at
Mlf2
BC003975
3.56

1450560_a_at
Ppp2r5d
NM_009358
3.56

1430070_at
1500035N22Rik
AK005363
3.56

1435472_at
Kremen1
BG069617
3.56

1419564_at
Zfp467
NM_020589
3.55

1426822_at
Rhot2
BM201133
3.55

1436085_at
Zbtb34
BB361974
3.55

1442019_at
Rcvrn
BB627097
3.55

1418135_at
Aff1
NM_133919
3.55

1425956_a_at
Cdadc1
BC004588
3.55

1416600_a_at
Dscr1
AF282255
3.54

1425455_a_at
Churc1
BC019472
3.54

1437214_at
Lrrtm4
BB667279
3.54

1457097_at
Skap2
BB212597
3.54

1433583_at
Zfp365
AV327248
3.54

1434567_at
4732496O08Rik
BE952228
3.54

1438231_at
Foxp2
AV322952
3.54

1456697_x_at
Dmtf1
BB248138
3.54

1419173_at
Acy1
NM_025371
3.54

1435200_at
6330419J24Rik
AV327542
3.53

1447470_at
Hdhd2
BF451327
3.53

1440212_at
Slc12a1
AI788571
3.53

1436387_at
Homer1
BB398124
3.53

1429738_at
Myt11
AK013847
3.53

1438379_x_at
2310007F21Rik
AV330311
3.53

1436483_at
Myt11
BM116113
3.53

1460701_a_at
Mrpl52
AV021593
3.53

1425510_at
Mark1
BM213279
3.52

1447538_at
Crtc1
AW124625
3.52

1415888_at
Hdgf
NM_008231
3.52

1423161_s_at
Spred1
BQ044290
3.52

1448691_at
Ubqln4
BB764994
3.52

1424810_at
Tasp1
BC005776
3.52

1451751_at
Ddit4l
AF335325
3.52

1421101_a_at
Ldb2
NM_010698
3.52

1460652_at
Esrra
NM_007953
3.52

1423081_a_at
Tomm20
AK002902
3.51

1424191_a_at
Tmem41a
BC019770
3.51

1425059_at
Prmt6
BC022899
3.51

1419754_at
Myo5a
NM_010864
3.51

1429218_at
Det1
BE457100
3.51

1422569_at
Yy1
BI665246
3.50

1450844_at
Stx6
BQ174465
3.50

1451908_a_at
Sec14l1
BC005766
3.50

1448224_at
Tfam
NM_009360
3.50

1448170_at
Siah2
AA414485
3.50

1455885_at
Gna12
AV238106
3.50

1455058_at
Mtmr9
BM118290
3.50

1460702_at
Triap1
AK007514
3.50

1449633_s_at
Nt5c31
AI841843
3.50

1420708_at
Traip
AK012948
3.50

1449472_at
Gpr12
NM_008151
3.50

1448889_at
Slc38a4
NM_027052
3.49

1420530_at
Neud4
AW553317
3.49

1418168_at
Zcchc14
BB223737
3.49

1447856_x_at
Slc25a25
BB186319
3.49

1449877_s_at
Kifc1
NM_016761
3.49

1448947_at
2810004N23Rik
NM_025615
3.49

1459372_at
Npas4
AV348246
3.48

1422463_a_at
Mrpl3
AB049633
3.48

1434693_at

BE952823
3.48

1437929_at
Dact2
AV306847
3.48

1423461_a_at
Ubl3
AV328436
3.48

1454773_at
Rxra
BQ175050
3.48

1442153_at
Uty
BB206087
3.48

1419683_at
Trp53rk
BB400773
3.48

1435141_at
Sft2d2
AV238378
3.48

1422487_at
Smad4
AK004804
3.48

1421493_a_at
Rgs20
NM_021374
3.48

1449392_at
Hsd17b1
NM_010475
3.48

1437524_x_at
Coro7
BB534801
3.47

1420545_a_at
Chn1
NM_029716
3.47

1419445_s_at
Sap18
NM_009119
3.47

1426984_at
2310067B10Rik
BC007157
3.47

1455609_at
C030025P15Rik
BB525143
3.46

1418172_at
Hebp1
AF117613
3.46

1447959_at
Zfp398
AI506402
3.46

1435063_at
RP23-25C1.8
AV326964
3.46

1449140_at
Nudcd2
BC005646
3.46

1419816_s_at
Errfi1
AI788755
3.46

1443129_at

BB363699
3.46

1453433_at
Wdr89
AK011125
3.46

1418412_at
Tpd52l1
NM_009413
3.46

1429417_at
4833446K15Rik
AK019528
3.45

1434755_at
Coro2b
BB538661
3.45

1420374_at
Foxj2
BI249549
3.45

1424575_at
Rabl5
BC009150
3.45

1433703_s_at
Bahd1
BM115038
3.45

1442268_a_at

BB749041
3.45

1429573_at
Dmrtc1a
AK014934
3.45

1448520_at
Dclre1b
NM_133865
3.45

1455230_at
Cacng4
BQ176350
3.45

1451717_s_at
Senp2
AV317107
3.44

1436690_at
Lrba
BB494139
3.44

1415950_a_at
Pebp1
NM_018858
3.44

1422486_a_at
Smad4
AK004804
3.44

1428526_at
Lsmd1
AK018782
3.43

1423038_at
Stx6
BQ174465
3.43

1435547_at

BQ175722
3.43

1443172_at
Orc11
BB210535
3.43

1453262_at
2810032G03Rik
AK012851
3.43

1434969_at
Brunol5
BB381558
3.43

1450326_at
Shc3
NM_009167
3.43

1456786_at
Ldb2
BB097063
3.43

1424416_at
Nkiras2
BC024398
3.43

1455708_at
Tmod3
BB749726
3.43

1425846_a_at
Caln1
AF282251
3.42

1418082_at
Nmt1
BC016526
3.42

1434183_at
LOC666704
BI415031
3.42

1451592_at
P42pop
AF364868
3.42

1434255_at
Pacs2
AV145060
3.42

1424175_at
Tef
BC017689
3.42

1417626_at
Pde4dip
NM_031401
3.42

1425479_at
Smyd5
BF160651
3.41

1460592_at
Epb4.1l1
BI684958
3.41

1424005_at
B230219D22Rik
BC006718
3.41

1456579_x_at
Telo2
BB462079
3.41

1434928_at
Gas2l1
BB769886
3.41

1416050_a_at
Scarb1
NM_016741
3.40

1420158_s_at
Abcf1
AA408356
3.40

1452684_at
Akt1s1
BM068933
3.40

1441264_x_at
A930005H10Rik
AV009179
3.40

1460428_at
Ankrd13a
BC003286
3.40

1456682_at
Lonrf2
AV221843
3.40

1416730_at
Rcl1
BC004574
3.40

1418323_at
Fem1b
BM232562
3.39

1436786_at
Sec14l3
AV024133
3.39

1451971_at
Cul4a
BC024113
3.39

1434085_at
Zfp523
AV136654
3.39

1429394_at
A130010J15Rik
BB025778
3.39

1433526_at
Klhl8
BM942651
3.39

1429745_at
LOC574405
AK005439
3.39

1436285_at
Ccdc113
AV279772
3.39

1433522_at
Pskh1
BG073986
3.39

1440286_at
Fbxl16
BM232403
3.38

1416977_at
Stam2
BB125321
3.38

1447868_x_at
Txnl2
BB030680
3.38

1440278_at
Dynll1
BM939312
3.38

1448757_at
Pml
NM_008884
3.38

1434546_at
Smg5
BI106637
3.38

1448785_at
Runx1t1
BG072085
3.38

1449956_at
Prkce
NM_011104
3.38

1437037_x_at
Snd1
BB240087
3.37

1456606_a_at
Phactr1
AV259240
3.37

1457703_at
Cacna2d4
BE952838
3.37

1460676_at
Josd1
BC006928
3.37

1451046_at
Zfpm1
AA014267
3.37

1436525_at
Ap3s2
AV225391
3.37

1417710_at
Mettl9
NM_021554
3.37

1418250_at
Arfl4
NM_025404
3.37

1433680_x_at
Siva1
AV216351
3.37

1434922_at
Phf12
BB483595
3.37

1424480_s_at
Akt2
BC026151
3.37

1427899_at
Rnf6
BI738010
3.37

1445685_at
Gas7
AI837851
3.36

1454612_at
Rkhd2
BI656279
3.36

1452323_at
Spryd3
BQ176406
3.36

1421498_a_at
2010204K13Rik
NM_023450
3.36

1456427_at
Gp1bb
BE988990
3.36

1420650_at
Atbf1
NM_007496
3.36

1459621_at
Itsn1
BB713496
3.36

1453412_a_at
Sec14l1
BI652727
3.35

1424195_a_at
Inpp5d
AF125996
3.35

1450710_at
Jarid2
NM_021878
3.35

1430614_at
4632415K11Rik
BG962576
3.35

1452015_at
6330416G13Rik
AV326978
3.35

1436764_at
Pard3
BE199556
3.35

1419470_at
Gnb4
BI713933
3.35

1429774_a_at
Ccdc98
AK014420
3.35

1434831_a_at
Foxo3a
BB364488
3.35

1415975_at
Carhsp1
AU080787
3.35

1418924_at
Rassf7
NM_025886
3.35

1437300_at
Mef2d
BG067616
3.34

1421175_at
Myt11
NM_008666
3.34

1428712_at
Mon1b
AK017167
3.34

1418080_at
B4galt2
AW125856
3.34

1438941_x_at
Ampd2
AV330806
3.34

1426679_at
Zfp706
AK004076
3.34

1418462_at
Exosc9
NM_019393
3.34

1416027_at
Pdcd6
NM_011051
3.34

1438313_at

BB233366
3.34

1456236_s_at
Commd10
AU019438
3.34

1425465_a_at
Senp2
AV317107
3.34

1428148_s_at
Coro7
BB203098
3.34

1418235_at
Atg5
AV168389
3.34

1415749_a_at
Rragc
NM_017475
3.34

1450539_at
Krtap5-1
NM_015808
3.34

1431320_a_at
Myo5a
AK002362
3.34

1439452_x_at
Dnpep
BB407163
3.33

1418255_s_at
Srf
BI662291
3.33

1442093_at
Adc
AU040368
3.33

1455056_at
Lmo7
BM231903
3.33

1427579_at
Rhbdl3
BE653576
3.33

1435170_at
Tsr2
BQ177187
3.33

1422751_at
Tle1
NM_011599
3.32

1459679_s_at
Myo1b
AA406997
3.32

1447449_at

BE983114
3.32

1453183_at
1110034A24Rik
BB815668
3.32

1423228_at
B4galt6
BG066773
3.32

1423660_at
Ctdsp2
BB294133
3.32

1416870_at
Dnalc4
NM_017470
3.32

1437597_at

BB182944
3.32

1415710_at
Cox18
BM123013
3.32

1447831_s_at
Mtmr7
BB431693
3.32

1422901_at
Mgea5
NM_023799
3.31

1423269_a_at
Nedd4l
BB663717
3.31

1423350_at
Socs5
AA510713
3.31

1451938_a_at
Sntb1
BC003748
3.31

1427878_at
0610010O12Rik
AK002512
3.31

1436617_at
Cetn4
AV211098
3.31

1457790_at
Asb3
BB002295
3.31

1441998_at

BB369709
3.31

1434706_at
Vcpip1
AV175609
3.31

1428950_s_at
Nol8
AK017551
3.31

1435974_at
Arhgef9
BB271482
3.31

1419650_at
Zfr
NM_011767
3.30

1443827_x_at
BC004044
BB375974
3.30

1460418_x_at
H2-T18
X03052
3.30

1426108_s_at
Cacnb1
AY094172
3.30

1460021_at
EG626231
BB825323
3.30

1432972_at
4921518B13Rik
AK014916
3.29

1438727_at
Wdr32
BB317629
3.29

1419351_a_at
l7Rn6
BC003916
3.29

1429730_at
1810009N02Rik
AK007409
3.28

1417311_at
Crip2
NM_024223
3.28

1424315_at
1110004E09Rik
BC019533
3.28

1448057_at

BI320076
3.28

1438932_at
Rasgrp2
BE688720
3.28

1455947_at
AK162044
BB667208
3.28

1423064_at
Dnmt3a
BB795491
3.27

1435213_at
Nhlrc1
BB309133
3.27

1420377_at
St8sia2
BG071333
3.27

1433960_at
Isg20l2
BB546077
3.27

1454642_a_at
Commd3
BB230296
3.27

1450143_at
Rasgrp1
BB354696
3.27

1445668_at
Tbce
BE993759
3.27

1450106_a_at
Evl
NM_007965
3.27

1428903_at
3110037I16Rik
AK014134
3.27

1437730_at
Ppp2r2a
C78402
3.27

1417349_at
Pldn
NM_019788
3.26

1418038_s_at
Dusp19
NM_024438
3.26

1424389_at
Nupl1
BC026743
3.26

1420870_at
Mllt10
BB353133
3.26

1441856_x_at
Tysnd1
AV259286
3.26

1448747_at
Fbxo32
AF441120
3.26

1424640_at
Arl8a
BC018479
3.26

1455775_at
B230315N10Rik
AW988196
3.26

1451106_at
Tut1
BC025499
3.26

1435505_at
Dmwd
BB698273
3.26

1438480_a_at
Vps26b
AV301675
3.26

1428165_at
Vps24
AK009414
3.25

1416126_at
Rpo1-2
NM_009086
3.25

1451178_at
Mrpl53
BC022162
3.25

1417919_at
Ppp1r7
NM_023200
3.25

1446477_at
Zfp622
BB038030
3.25

1425081_at
Zfp286
BE651907
3.25

1427564_at
Diap2
Y15910
3.25

1420643_at
Lfng
NM_008494
3.25

1425396_a_at
Lck
BC011474
3.25

1432096_at
Snrpn
AK010671
3.24

1442524_at

AI836848
3.24

1446556_at
Kcnd1
BB073575
3.24

1420034_at
Ppp2r2d
AU019644
3.24

1422083_at
Tlr9
AF348140
3.23

1437262_x_at
Bcas2
AA940256
3.23

1437861_s_at
Prkce
BB335101
3.23

1424332_at
Rab40c
AF422144
3.23

1435604_at
Trim37
BM941778
3.23

1435527_at
Nfic
BB533448
3.23

1454871_at
Rbm15b
BB776868
3.23

1436138_at
Ttc19
BB078627
3.23

1423923_a_at
Wdr8
BC016120
3.23

1447249_at

BF149076
3.23

1426675_at
Tomm70a
BB225670
3.23

1435225_s_at
Brpf3
BB728354
3.23

1428554_a_at
1810035L17Rik
BQ126239
3.22

1437481_at
1110039F03Rik
AV032349
3.22

1426398_at
Ube2w
BB796558
3.22

1440829_x_at
Ttll11
AV266869
3.22

1460191_at
Ykt6
NM_019661
3.22

1436383_at
Cplx2
BE946238
3.22

1421817_at
Gsr
AK019177
3.22

1438423_at
Ssbp2
BB085949
3.21

1452214_at
Skil
AK018608
3.21

1428672_at
Snrpf
BI080799
3.21

1440997_at
Tnrc6c
AV236734
3.21

1454873_at
Zfp775
BB667577
3.21

1418986_a_at
Uxt
NM_013840
3.21

1434058_at
Mtmr12
BB667459
3.21

1417044_at
Lcmt1
NM_025304
3.21

1435961_at
Nat14
BB702376
3.21

1428189_at
5730494M16Rik
BQ174627
3.21

1442883_s_at
D10Bwg1364e
BI413749
3.20

1432478_a_at
Ibrdc3
AK015966
3.20

1421968_a_at
Nipa2
NM_023647
3.20

1434476_at
Crtc1
BM119896
3.20

1433922_at
Rab35
BG074549
3.19

1428520_at
1110032A13Rik
AK004019
3.19

1419221_a_at
Rgs14
NM_016758
3.19

1423317_at
3110001D03Rik
BE915283
3.19

1450746_at
Keap1
AW764104
3.19

1415792_at
Rbck1
NM_019705
3.19

1452958_at
Asphd2
AK013495
3.19

1418763_at
Nit2
BC020153
3.19

1419081_at
Atg10
NM_025770
3.18

1449688_at
D10Ertd610e
AU014694
3.18

1423873_at
Lsm1
BC021460
3.18

1431049_at
Ica11
BM964047
3.18

1416501_at
Pdpk1
NM_011062
3.18

1438506_s_at
Abi1
AV330821
3.18

1434564_at
E2f3
BQ176318
3.18

1416432_at
Pfkfb3
NM_133232
3.17

1445475_at
Pak6
BB003750
3.17

1417461_at
Cap1
NM_007598
3.17

1428924_at
Mocs3
AU016306
3.17

1422605_at
Ppp1r1a
NM_021391
3.17

1451680_at
Srxn1
BC011325
3.17

1427468_at
Ppp3cb
M81483
3.17

1455172_at
AU020094
BG069639
3.17

1417368_s_at
Ndufa2
NM_010885
3.17

1444774_at
Det1
BM123155
3.17

1447707_s_at
Pde2a
BB367174
3.17

1429095_at
Cenpp
AK006234
3.16

1455289_at
Ankrd13b
BQ174304
3.16

1431179_at
Entpd7
BB873170
3.16

1437715_x_at
Apex1
AV100480
3.16

1434243_s_at
Tomm70a
AV233564
3.15

1424062_at
Ube2d1
BC019464
3.15

1435780_at
Psd
BG966595
3.15

1437363_at
Homer1
BQ043238
3.15

1442799_x_at
Nudcd3
BB048987
3.15

1455881_at
Ier5l
BB078200
3.15

1449116_a_at
Dtymk
NM_023136
3.15

1438217_at
A2bp1
AW494804
3.15

1421845_at
Golph3
AV174110
3.15

1429929_at
Mei1
AK016514
3.15

1429072_at
1110001D15Rik
AK003211
3.14

1429715_at
Ppp2r2a
AK010380
3.14

1421176_at
Rasgrp1
BB354696
3.14

1420449_at
Heatr5b
NM_028084
3.14

1455542_at
C630043F03Rik
BB226174
3.14

1422441_x_at
Cdk4
NM_009870
3.14

1437921_x_at
Zfp516
AW744723
3.14

1429108_at
Msl211
BB745314
3.14

1423216_a_at
2510049I19Rik
AV109006
3.14

1417065_at
Egr1
NM_007913
3.14

1449007_at
Btg3
NM_009770
3.14

1460352_s_at
Pik3r4
BC017537
3.13

1437010_a_at
Oaz3
BF319714
3.13

1425599_a_at
Gatad1
BC019449
3.13

1424047_at
Dera
BC016218
3.13

1426861_at
Aftph
BC004630
3.13

1455771_at
Bzrap1
BB467799
3.13

1428824_at
2310003C23Rik
AK009106
3.13

1441890_x_at
Tmeff1
BB366425
3.13

1436648_at
Nanos1
BB251623
3.13

1428267_at
Dhx40
AK010512
3.13

1433720_s_at
Ndg2
AI647775
3.13

1422526_at
Acsl1
BI413218
3.13

1450992_a_at
Meis1
AW547821
3.13

1432271_a_at
Dcun1d5
AK012764
3.13

1448989_a_at
Myo1b
AI255256
3.13

1424264_at
Med6
BC013096
3.13

1454978_at
Ttyh3
BQ180649
3.12

1421500_at
Sts
NM_009293
3.12

1438416_at
Thrap5
BM238407
3.12

1434304_s_at
D10Ertd438e
BE949414
3.12

1437141_x_at
Dym
BB549381
3.12

1416622_at
Wbscr16
NM_033572
3.12

1452175_at
1810026J23Rik
BB027373
3.12

1428926_at
1110003O08Rik
AK003388
3.12

1456946_at
Sh3md4
BE949337
3.12

1449413_at
Mpv17l
NM_033564
3.12

1428081_at
Klhl21
AK007786
3.12

1447934_at
9630033F20Rik
BB128741
3.12

1459998_at
Zfp407
AW547438
3.12

1416299_at
Shcbp1
NM_011369
3.12

1426247_at
Stk24
BG060677
3.12

1422485_at
Smad4
AK004804
3.11

1433419_at
4930405A07Rik
AK015090
3.11

1459751_s_at
Ppp1r16a
R75527
3.11

1418575_at
Shfm1
NM_009169
3.11

1428327_at
Trak1
BB010301
3.11

1428358_at
1810010M01Rik
BF581690
3.11

1416743_at
Uap1
NM_133806
3.11

1445691_at
Chn1
AW121426
3.11

1452593_a_at
Tceb1
AI019214
3.11

1439024_at
Bag4
BB082119
3.11

1418574_a_at
Shfm1
NM_009169
3.11

1451288_s_at
1810043G02Rik
BC010330
3.11

1419773_at
2010301N04Rik
BB080387
3.11

1416324_s_at
Kctd20
AV281062
3.11

1427309_at
Pars2
BF583012
3.11

1449634_a_at
Anks1b
AW046296
3.11

1454032_at
Neto2
AK017458
3.11

1419568_at
Mapk1
NM_011949
3.10

1418416_x_at
Psg23
NM_020261
3.10

1428658_at
Pin4
AW012413
3.10

1437743_at
Aebp2
BM242614
3.10

1428533_at
D1Bwg0212e
BG918881
3.10

1455407_at
Zfp236
BB282741
3.10

1425781_a_at
Plcb1
U85714
3.10

1419984_s_at
Zfp644
AU015726
3.10

1435728_at
Tyw3
AW457809
3.10

1428474_at
Ppp3cb
AK004360
3.10

1419995_at
D10Ertd641e
AU015124
3.10

1435476_a_at
Fcgr2b
BM224327
3.10

1429382_at
Tomm40l
AV253376
3.09

1417084_at
Eif4ebp2
NM_010124
3.09

1417176_at
Csnk1e
BE952001
3.09

1457661_at
B930036G03Rik
BB405928
3.09

1445677_x_at
Slc35f2
BB401264
3.09

1439408_a_at
Pparbp
AV100992
3.09

1427212_at
Mapkap1
BC027377
3.09

1455204_at
Pitpnc1
BB305507
3.09

1459906_at
Dgkh
BB655998
3.09

1438306_at
Rnf180
AV340072
3.09

1418144_a_at
Pip5k1b
NM_008847
3.09

1416767_a_at
1110003E01Rik
NM_133697
3.09

1428630_x_at
Haghl
AK021220
3.09

1450685_at
Arpp19
BE648432
3.08

1436731_at
Zfp533
BB333374
3.08

1438561_x_at
Tmem180
BB400326
3.08

1416545_at
Zdhhc7
NM_133967
3.08

1436122_at
Zfp667
BF467246
3.08

1424317_at
Slc25a19
BC018167
3.08

1432447_a_at
2310005N01Rik
AK009177
3.08

1458322_x_at
E230008N13Rik
BB139156
3.08

1416281_at
Wdr45l
BC004595
3.08

1452026_a_at
Pla2g12a
AY007382
3.08

1444388_at

BB020727
3.08

1428665_at
Pfn4
BQ174081
3.08

1416292_at
Prdx3
NM_007452
3.08

1416394_at
Bag1
NM_009736
3.07

1448611_at
Wwc2
NM_133791
3.07

1454996_at
Hsdl1
BE985436
3.07

1441356_at
Shc2
BB053802
3.07

1437573_at

BF018351
3.07

1428593_at
1700029F09Rik
BG095162
3.07

1424527_at
Ppp2r2d
AF366393
3.07

1423519_at
2210412D01Rik
BE457744
3.07

1424163_at
Rmnd5b
BC016075
3.07

1454797_at
Tmem55b
AW556098
3.06

1448724_at
Cish
NM_009895
3.06

1437452_x_at
Vdac1
AV008054
3.06

1443821_at
Lect2
AW122758
3.06

1428585_at
Actn1
BE853286
3.06

1426915_at
Dapk1
BC021490
3.06

1439611_at
Chrm1
BE947522
3.06

1452933_at
Ankrd39
AK018681
3.06

1428215_x_at
Tomm7
BB609428
3.06

1418656_at
Lsm5
NM_025520
3.06

1420390_s_at
Zfp354a
NM_009329
3.05

1432295_a_at
2310035K24Rik
AK009634
3.05

1421123_at
Cdk5r1
NM_009871
3.05

1443688_at
BC050092
BB710844
3.05

1460372_at
Duoxa1
BC019755
3.05

1423967_at
Palm
BC015297
3.05

1436329_at
Egr3
AV346607
3.05

1440343_at
Rps6ka5
BQ174267
3.05

1427236_a_at
Mll5
BB295149
3.05

1447882_x_at
Ddx54
AV318690
3.05

1420219_at
4930461P20Rik
BB192718
3.05

1426572_at
Me2
BM235734
3.05

1460116_s_at
Spred1
AI450584
3.05

1425718_a_at
Ivns1abp
BC004092
3.04

1447762_x_at
Car12
BB503164
3.04

1416664_at
Cdc20
NM_023223
3.04

1437679_a_at
Glrx2
BB172698
3.04

1459994_x_at
Trfr2
AV027486
3.04

1452287_at

BB414973
3.04

1435900_at
Zbtb43
BF466570
3.04

1415945_at
Mcm5
NM_008566
3.04

1424330_at
Senp1
BC023129
3.03

1418300_a_at
Mknk2
NM_021462
3.03

1418174_at
Dbp
BC018323
3.03

1420833_at
Vamp2
BG871810
3.03

1452046_a_at
Ppp1cc
BG071790
3.03

1452438_s_at
Taf4a
BE632382
3.03

1422493_at
Cpox
BG067254
3.03

1429094_at
Ddi2
BI110088
3.03

1427067_at
4933439F18Rik
AJ404329
3.03

1426752_at
Phf17
BG065238
3.03

1419377_at
Med9
BC019367
3.02

1435067_at
B230208H17Rik
BG075288
3.02

1430305_at
2500004C02Rik
AK010870
3.02

1443796_at
Il16
BB206877
3.02

1435226_at
Ibrdc3
BG064140
3.02

1431352_s_at
Pvt1
BI453402
3.02

1428192_at
Kbtbd7
AK003597
3.02

1434033_at
Tle1
AI325112
3.02

1417840_at
1500031L02Rik
NM_025892
3.02

1452275_at
Zfand3
BG976649
3.02

1424200_s_at
Seh11
AW537349
3.02

1428763_at
Smug1
AK004735
3.02

1421395_at
Zik1
NM_009577
3.02

1419827_s_at
Kif17
AW492270
3.02

1433603_at
Ndufs6
C88200
3.02

1431875_a_at
E2f1
AK017841
3.02

1453837_at
6330500D04Rik
BB867666
3.02

1435714_x_at
Il17d
BB809808
3.01

1417360_at
Mlh1
NM_026810
3.01

1416614_at
Eid1
BC010712
3.01

1453348_at
1700085D22Rik
AK007007
3.01

1453199_at
Acbd6
AK011781
3.01

1431581_at
4922502B01Rik
AK013705
3.01

1456183_at
Oog4
BB555196
3.01

1421768_a_at
Homer1
NM_011982
3.01

1433946_at
Zik1
BE824681
3.01

1420849_at
Crnkl1
AV143435
3.01

1453274_at
Ccdc103
AK006639
3.01

1440005_at
Onecut2
BB667396
3.01

1439434_x_at
Pcmt1
BB317673
3.01

1460692_at
Ehmt2
BI412952
3.01

1422763_at
Gipc1
NM_018771
3.01

1455174_at
Rps19bp1
AV107461
3.00

1436381_at
Dlgap3
BQ175774
3.00

1416991_at
Mto1
NM_026658
3.00

1423358_at
1810009K13Rik
AK007407
3.00

1430129_a_at
Commd8
AK017777
3.00

1448956_at
Stard10
NM_019990
3.00

1416439_at
2410015N17Rik
NM_023203
3.00

1439869_at
2900054J07Rik
BB326106
3.00

1428550_at
1810015A11Rik
AK007503
3.00

1439493_at
D630040G17Rik
BB111712
3.00

1441960_x_at
5730494M16Rik
AV267590
2.99

1426465_at
Dlgap4
BG066219
2.99

1429438_at
Bcor
AV318805
2.99

1434848_at

BB259283
2.99

1437729_at
Rpl27a
BG141806
2.99

1457114_at
Tor1b
BB475906
2.99

1426532_at
Zmynd11
BB832996
2.99

1437651_a_at
Dtnb
AW537479
2.99

1449979_a_at
Spock3
NM_023689
2.99

1424790_at
Slc25a42
BC025937
2.99

1425848_a_at
Dusp26
BC018204
2.99

1452925_a_at
4-Mar
AK009364
2.99

1429111_at
Tln2
AV270892
2.99

1423285_at
Coch
BB731671
2.98

1436608_at
BC038822
BB457436
2.98

1422762_at
Kif17
AB008867
2.98

1443979_at
A830021K08Rik
AI851680
2.98

1438166_x_at
Ndufs4
AV219958
2.98

1439867_at
Ubox5
BB079377
2.98

1420885_a_at
Sez6
NM_021286
2.98

1416337_at
Uqcrb
NM_026219
2.98

1425466_at
Senp2
AV317107
2.98

1436055_at
Lrrc15
AI506528
2.98

1427934_at
Lyrm2
AA250510
2.98

1452351_at
C030027K23Rik
BC025847
2.97

1460703_at
Ascc1
AK007519
2.97

1418034_at
Mrps9
BC022587
2.97

1448849_at
Mrpl40
NM_010922
2.97

1422036_at
Strn
NM_011500
2.97

1442881_at

BF462648
2.97

1417305_at
Speg
NM_007463
2.97

1460165_at
Ppp1ca
NM_031868
2.97

1446681_at
BB086117
AV327329
2.97

1439838_a_at
Tmie
BE957021
2.97

1426742_at
Atp5f1
AK019459
2.97

1423063_at
Dnmt3a
BB795491
2.97

1416418_at
Gabarapl1
AF180518
2.97

1431304_a_at
Tmem183a
AU080745
2.97

1448842_at
Cdo1
NM_033037
2.97

1454898_s_at
Iah1
AU016407
2.97

1455105_at
Ptpn12
AV117227
2.96

1452530_a_at
Runx1
X97306
2.96

1426640_s_at
Trib2
BB354684
2.96

1433761_at
Pde4dip
AV374669
2.96

1451996_at
Tm2d1
AF353993
2.96

1428494_a_at
Cox7a1
BB284638
2.96

1456366_at
1700015F17Rik
BB833901
2.96

1420103_at

AA589431
2.96

1452878_at
Prkce
AK017901
2.96

1427460_at
Taf4a
BE632382
2.96

1442408_at
2010305B15Rik
BE981170
2.96

1454803_a_at
Hdac11
BB183559
2.96

1435865_at
Hist3h2a
AV297651
2.96

1429918_at
Arhgap20
AK018317
2.96

1452607_at
2610030H06Rik
BB410125
2.96

1422494_s_at
Ccrk
NM_053180
2.96

1435260_at
Akt3
BG695418
2.95

1438985_x_at
Otud5
BB158937
2.95

1434487_at
Mef2d
AV334508
2.95

1425893_a_at
Fhit
AF055573
2.95

1429273_at
Bmper
AK014221
2.95

1427990_at
Usp45
BM249286
2.95

1416528_at
Sh3bgrl3
NM_080559
2.95

1448027_at
2010305B15Rik
AI428254
2.95

1418325_at
Sephs2
NM_009266
2.95

1427112_at
Ttl
AV127581
2.95

1439192_at
Nova2
BB524078
2.94

1418434_at
Mkrn1
BQ176661
2.94

1458438_at
Ccdc122
AW554226
2.94

1449590_a_at
Mras
AB004879
2.94

1419960_at
Anxa11
AU019881
2.94

1456037_x_at
Preb
BB225012
2.94

1423661_s_at
Ctdsp2
BB294133
2.94

1454711_at
Trio
BB080177
2.94

1441120_at
Ceacam16
BB268577
2.94

1449147_at
Chst1
NM_023850
2.94

1457719_at

AV230618
2.94

1448951_at
Tnfrsf1b
M60469
2.94

1460434_at
Fundc2
AI510221
2.94

1428483_a_at
2610039C10Rik
AK012762
2.94

1457513_at
6330569M22Rik
BB525203
2.94

1423222_at
Cap2
AV261931
2.94

1424514_at
Rnf126
BC016543
2.94

1416286_at
Rgs4
NM_009062
2.93

1428864_at
5530400B01Rik
AK017419
2.93

1441921_x_at
Esrrb
BB386239
2.93

1424145_at
Prr3
BC016636
2.93

1455412_at
9330180L21Rik
BG064966
2.93

1426475_at
Hmbs
AI325144
2.93

1416657_at
Akt1
NM_009652
2.93

1449338_at
D10Ertd641e
BE692118
2.93

1448218_s_at
Ywhaz
AV027921
2.93

1434365_a_at
BC055324
BB093351
2.93

1454759_at
Git1
AV230461
2.92

1422976_x_at
Ndufa7
NM_023202
2.92

1440555_at
Rragd
BB354941
2.92

1423551_at
Cdh13
BB776961
2.92

1451028_at
Baiap2
AA796998
2.92

1436284_s_at
Zfp319
BE949045
2.92

1453077_a_at
Snapc3
AK016168
2.92

1415794_a_at
Spin1
BM228780
2.92

1434345_at
Clrn3
BB496366
2.92

1436232_a_at
Gabpb1
BQ177003
2.92

1457648_x_at
BC004044
BB084182
2.92

1439465_x_at
9430057O19Rik
AV277339
2.92

1451037_at
Ptpn9
BB224063
2.92

1417032_at
Ube2g2
AF296657
2.92

1429623_at
Zfp644
AV261187
2.92

1416194_at
Cyp4b1
NM_007823
2.92

1445461_at

BB096245
2.91

1422414_a_at
Calm2
NM_007589
2.91

1450448_at
Stc1
BQ032752
2.91

1430046_at
Otud3
AK014098
2.91

1451044_at
Sip1
AK013414
2.91

1426234_s_at
BC002199
BC002199
2.91

1450084_s_at
Ivns1abp
NM_054102
2.91

1420340_at
Cspp1
NM_026493
2.90

1456079_x_at
Apex1
AV263745
2.90

1424423_at
Lenep
BC006806
2.90

1427467_a_at
Rpgr
AJ238396
2.90

1435422_at
4933433P14Rik
AV281088
2.90

1450690_at
Ranbp2
BM507707
2.90

1416345_at
Timm8a1
W82151
2.90

1429306_at
Lzic
AK007657
2.90

1428979_at
Mtf1
AV316320
2.90

1420107_at
Bbs4
C86773
2.90

1437680_x_at
Glrx2
BB172698
2.90

1448705_at
Zbtb22
BC026964
2.90

1456072_at
Ppp1r9a
AU067663
2.90

1425600_a_at
Plcb1
AW049748
2.90

1455755_at
Gm88
BB026554
2.89

1433931_at
C030046I01Rik
AW553381
2.89

1434169_at
9030409G11Rik
BQ175107
2.89

1425777_at
Cacnb1
AY094173
2.89

1430803_at
Lin28b
BB074761
2.89

1421170_a_at
Plcb1
BB215274
2.89

1423200_at
Ncor1
U22016
2.89

1451409_at
2210021J22Rik
BC025858
2.89

1426518_at
Tubgcp5
AV333951
2.89

1446768_at

BE653578
2.89

1438632_x_at
Tnp1
AV206780
2.89

1456582_x_at
LOC637796
BB024498
2.89

1425224_at
Slmo1
BC019561
2.89

1453380_a_at
Xrcc6bp1
AK010471
2.88

1455281_at
Wdr33
BB520667
2.88

1415748_a_at
Dctn5
NM_021608
2.88

1419690_at
2610002M06Rik
NM_025921
2.88

1424785_at
Angptl6
BC025904
2.88

1457695_at
Ap1gbp1
C76297
2.88

1452051_at
Actr3
BE372352
2.88

1425452_s_at
AW125753
BC002154
2.88

1449476_at
Rage
NM_011973
2.88

1426404_a_at
Rnf11
BI150320
2.88

1432332_a_at
Nudt19
AK008430
2.88

1457378_at
Mrpl54
AW123022
2.88

1419144_at
Cd163
NM_053094
2.87

1435549_at
Trpm4
BI685685
2.87

1449746_s_at
Krr1
AU020154
2.87

1441947_x_at
BC033915
BB327032
2.87

1451036_at
Spg21
BB095517
2.87

1424884_at
Fbxw2
BC003834
2.87

1428293_at
2310022M17Rik
AK009485
2.87

1460020_at
AA930108
W14742
2.87

1419937_at
AA536749
C76423
2.87

1426780_at
D14Ertd436e
AV247381
2.87

1415743_at
Hdac5
NM_010412
2.87

1457743_at
Rgs7bp
BQ175190
2.87

1423399_a_at
Yaf2
BG143658
2.87

1440127_a_at

AW123182
2.87

1426901_s_at
Camta2
BB657856
2.87

1425050_at
Isoc1
AK010892
2.87

1417854_at
Map2k5
NM_011840
2.86

1451450_at
2010011I20Rik
AK008190
2.86

1426850_a_at
Map2k6
BB261602
2.86

1420774_a_at
4930583H14Rik
NM_026358
2.86

1416574_at
5730589K01Rik
NM_023434
2.86

1451722_s_at
Smyd5
BF160651
2.86

1447897_x_at
Anapc11
AV019615
2.86

1452121_at
Fbxo22
BB756840
2.86

1434870_at
2810004N23Rik
BB121003
2.86

1441693_at
Adamts3
BB193360
2.86

1419763_at
2610020O08Rik
NM_025937
2.86

1430208_at
Krt42
AK019115
2.86

1456565_s_at
Map3k12
BB370469
2.86

1460364_at
Gtf2ird1
AI550317
2.86

1458386_at
C030009J22Rik
BB283503
2.86

1428321_at
Eml1
AK003593
2.86

1427449_a_at
Adprhl2
BC023177
2.86

1453204_at
4933431K14Rik
BB762387
2.86

1419955_at
Zfand3
AW539211
2.86

1456964_at
Rbm12
BB104271
2.86

1441937_s_at
Pink1
AV371921
2.86

1448184_at
Fkbp1a
AF483488
2.85

1416773_at
Wee1
NM_009516
2.85

1416824_at
B230118H07Rik
NM_026592
2.85

1433762_at
C630043F03Rik
BB182501
2.85

1433917_x_at
D2Bwg1423e
BB359828
2.85

1457270_at
Rcvrn
AI506234
2.85

1429561_at
Brf2
AK012680
2.85

1426669_at
C530044N13Rik
BC025476
2.85

1457610_at
Rbck1
BB281404
2.85

1428248_at
Nfx1
AK005038
2.85

1432543_a_at
Klf13
AK002926
2.85

1429162_at
1500015A07Rik
AK005240
2.85

1441892_x_at
4933440H19Rik
BB341238
2.85

1416106_at
Kti12
NM_029571
2.84

1446937_at
Bptf
AI450357
2.84

1416928_at
Rbm12
NM_029397
2.84

1457061_at
Glcci1
BB246045
2.84

1455942_at
Fbxl11
BB448023
2.84

1416562_at
Gad1
AF326547
2.84

1416128_at
Tuba1c
NM_009448
2.84

1416586_at
Zfp239
NM_008616
2.84

1458107_at

BB310489
2.84

1426912_at
Rfwd2
BB296692
2.84

1438557_x_at
Dnpep
BB553721
2.84

1418217_at
Nme7
NM_138314
2.84

1453564_a_at
Vps24
AK014818
2.84

1441305_at

BB360028
2.84

1435523_s_at
2700089E24Rik
BB779140
2.84

1439382_x_at
Ddr1
BB378700
2.83

1426209_at
Strn4
AF414080
2.83

1453064_at
Etaa1
AK018594
2.83

1439563_at
Zfp3
BB309203
2.83

1418463_at
Pik3r2
NM_008841
2.83

1426227_s_at
Vps37c
BC025865
2.83

1419583_at
Cbx4
NM_007625
2.83

1455897_x_at
Hmgn1
BF464696
2.83

1448897_at
Mkrn2
BI134671
2.83

1439770_at
6430598A04Rik
BB387298
2.83

1427296_at
BC010304
BB553912
2.83

1451177_at
Dnajb4
BC017161
2.83

1440242_at
C030014K22Rik
BB356428
2.83

1448227_at
Grb7
NM_010346
2.83

1438473_at
Arl15
BB445175
2.83

1428655_at
Ccdc128
AK003781
2.82

1455712_at
Hist3h2a
AI848909
2.82

1416924_at
Bri3
NM_018772
2.82

1440858_at
Crkrs
BB830220
2.82

1423486_at
Cript
BB775592
2.82

1427187_at
B3gntl1
AV319801
2.82

1417510_at
Vps4a
NM_126165
2.82

1427909_at
2410015M20Rik
BQ031065
2.82

1439106_at
Zfp462
AV320128
2.82

1451479_a_at
Tmem53
BC019937
2.82

1417448_at
1810008A18Rik
NM_133998
2.82

1434326_x_at
Coro2b
BB317923
2.81

1453846_at
Chd2
AK012891
2.81

1434820_s_at
Pkig
AV209892
2.81

1451073_at
Sppl3
BC023131
2.81

1453632_at
4930538K18Rik
BB548441
2.81

1460740_at
Cltb
AK009844
2.81

1426552_a_at
Bcl11a
BB772866
2.81

1455614_at
Tomm40l
AV225789
2.81

1434603_at
Thrap2
BG074645
2.80

1444106_at
D330012F22Rik
BB522739
2.80

1435156_at
BC046331
BF730275
2.80

1438469_at
Bcorl1
BG071122
2.80

1416036_at
Fkbp1a
AF483488
2.80

1458753_at
Gm941
BB548169
2.80

1449125_at
Tnfaip8l1
NM_025566
2.80

1453374_at
Zfand5
AW744618
2.80

1433450_at
Cdk5r1
BB177836
2.80

1447630_x_at
Gnb5
BB450318
2.80

1428852_at
Dock3
AK018140
2.80

1450557_at
Scn4a
NM_133199
2.80

1449040_a_at
Sephs2
NM_009266
2.80

1454781_x_at
Commd9
BB506415
2.80

1417363_at
Zfp61
NM_009561
2.80

1451403_at
BC024868
BC024868
2.80

1431270_a_at
Aste1
AK006153
2.79

1435589_at
Ccdc85b
AV308222
2.79

1424488_a_at
Ppa2
BC011417
2.79

1451098_at
Pcoln3
BC010524
2.79

1417918_at
Mrpl11
NM_025553
2.79

1450382_at
Nf2
L28176
2.79

1448874_a_at
Sh2b1
NM_011363
2.79

1458466_at
Ctsa
AV338634
2.79

1443859_at
Rsbn1
AI551821
2.79

1428150_at
Coro7
BB203098
2.79

1417265_s_at
Coq5
AK014348
2.79

1448845_at
Rpp25
NM_133982
2.79

1417657_s_at
Dnajc2
BG067003
2.79

1435625_at
Entpd7
BG064768
2.79

1427589_at
8430419L09Rik
BE283366
2.78

1422770_at
Rad51l3
AB052834
2.78

1453821_at
N6amt1
BQ033064
2.78

1422510_at
Ctdspl
NM_133710
2.78

1417207_at
Dvl2
BF466091
2.78

1424320_a_at
Traf7
BC008598
2.78

1451260_at
Aldh1b1
BC020001
2.78

1420199_at
Slc35a4
AV144937
2.78

1423304_a_at
Rnf111
BB366842
2.78

1451533_at
BC022687
BC022687
2.78

1445482_at
Gle1l
BB375481
2.78

1451518_at
Zfp709
BC021921
2.78

1416229_at
Rfk
NM_019437
2.78

1436086_at
4921526F01Rik
AV256234
2.78

1437150_at
1700012H17Rik
BB496291
2.78

1459981_s_at
Rsbn1
AV240926
2.78

1434481_at
4121402D02Rik
BI695596
2.78

1454885_at
BC063263
BM211194
2.77

1441534_at
C86753
BG067565
2.77

1437267_x_at
Hnrph1
BB164501
2.77

1418497_at
Fgf13
AF020737
2.77

1438898_at

BB309301
2.77

1433858_at
Lrrc28
BB667092
2.77

1416093_a_at
Mrpl20
NM_025570
2.77

1455185_s_at
Phf16
BG064477
2.77

1429053_at
1110012J17Rik
BB795266
2.77

1431164_at
Rragd
AK017818
2.77

1423512_at
AW209491
BF715189
2.77

1441317_x_at
Jakmip1
BB316060
2.77

1434217_at
C330019G07Rik
BB400635
2.77

1452119_at
2600005C20Rik
BG293527
2.77

1443724_at
Jph3
AI849712
2.77

1449947_s_at
Atbf1
NM_007496
2.77

1455774_at
Jund1
BB049005
2.77

1426818_at
Arrdc4
BC025091
2.77

1425053_at
Isoc1
AK010892
2.77

1418982_at
Cebpa
BC011118
2.77

1416448_at
Itpa
NM_025922
2.77

1415926_at
Nup62
NM_053074
2.77

1436808_x_at
Mcm5
AI324988
2.76

1420981_a_at
Lmo4
NM_010723
2.76

1436902_x_at
Tmsb10
BB096368
2.76

1454014_a_at
Mkks
AK004987
2.76

1417535_at
Fbxo25
NM_025785
2.76

1460665_a_at
Cnot7
NM_011135
2.76

1455272_at
Grm5
BB429139
2.76

1443822_s_at
Zcd1
AV347904
2.76

1429779_at
Eif2c4
AI481660
2.76

1425114_at
Rbbp6
BC025874
2.76

1435097_at
Mmab
BB772682
2.76

1449511_a_at
Ssbp4
NM_133772
2.75

1453305_at
Iqcd
AK017032
2.75

1420999_at
Cnot4
AI448404
2.75

1433780_at
Ubn1
BB463209
2.75

1451448_a_at
1110005A03Rik
BC026936
2.75

1459920_at
4933415E08Rik
AA163908
2.75

1426180_a_at
Smr2
U82380
2.75

1436845_at
Axin2
BB398993
2.75

1427970_at
Zfp689
AV226931
2.75

1427935_at
Lyrm2
AA250510
2.75

1453284_at
4930405M20Rik
AK015100
2.75

1443890_at

BE851943
2.75

1453010_at
Iws1
AK006925
2.75

1455673_at
Alkbh2
BG070741
2.75

1455169_at
Rab11fip2
BG076094
2.75

1419994_s_at
D10Ertd641e
AU015124
2.75

1452153_at
Fbxo18
AF184275
2.75

1415705_at
9130011J15Rik
AK018610
2.75

1440205_at
Zmynd19
BM195344
2.75

1451223_a_at
Btf3l4
AK011367
2.75

1420626_at
2410016F19Rik
BF782285
2.74

1459579_at
Cacng8
BQ266161
2.74

1426673_at
Cdh3
X06340
2.74

1415782_at
Sumo2
NM_133354
2.74

1421065_at
Jak2
NM_008413
2.74

1421191_s_at
Gopc
NM_053187
2.74

1439135_at

BB129618
2.74

1452271_at
Xpr1
AV337591
2.74

1420529_at
Neud4
AW553317
2.74

1416768_at
1110003E01Rik
NM_133697
2.74

1427879_at
1810031K17Rik
AK007667
2.74

1421147_at
Terf2
NM_009353
2.74

1452149_at
Ube3b
AU067784
2.74

1431753_x_at
Urm1
AK012124
2.74

1428775_at
1110008L16Rik
AK003589
2.73

1433673_at
E130309D14Rik
BG069691
2.73

1460568_at
Trim46
BI156583
2.73

1449109_at
Socs2
NM_007706
2.73

1426028_a_at
Cit
AF086823
2.73

1416805_at
1110032E23Rik
NM_133187
2.73

1433710_at
Edc3
BM208114
2.73

1420967_at
Slc25a15
AW541300
2.73

1459875_x_at
5730494M16Rik
AV333371
2.73

1420645_at
Pcgf2
NM_009545
2.73

1431939_a_at
Mina
AK014426
2.73

1453848_s_at
Zbed3
AK002774
2.73

1416224_at
Zbtb17
NM_009541
2.73

1443719_x_at
Ddx42
AW763628
2.73

1453387_at
4833432E10Rik
BB095161
2.73

1433992_at
Synpo
BQ176992
2.73

1434745_at
Ccnd2
BQ175880
2.73

1422800_at
Bat2
AK019427
2.73

1425478_x_at
Ube2i
U76416
2.72

1451254_at
Ikbkap
AF367244
2.72

1419174_at
2410004B18Rik
NM_025555
2.72

1443722_at
6030419C18Rik
BE945915
2.72

1456098_a_at
Elmo2
BG076364
2.72

1425557_x_at
Tsc22d3
AF201288
2.72

1419235_s_at
Helb
BG070273
2.72

1422758_at
Chst2
NM_018763
2.72

1448518_at
Timm22
AK012130
2.72

1453886_a_at
Slc25a26
AK017037
2.72

1426192_at
Smarcd2
M60510
2.72

1434630_at
Ankrd28
AV261293
2.72

1429908_at
6530403A03Rik
AK004216
2.72

1423750_a_at
Sf1
BC009091
2.72

1433939_at
Aff3
BQ177036
2.72

1426373_at
Ski
AK019148
2.72

1427071_at
Fbxo42
BC003960
2.71

1436561_at
Suv39h2
BB440055
2.71

1452179_at
Phf17
BG065238
2.71

1440029_at
St8sia3
BB379120
2.71

1454800_at
Morn2
BF319573
2.71

1421842_a_at
Scamp4
AF295102
2.71

1457472_at
Tnrc15
BB782031
2.71

1416351_at
Map2k1
NM_008927
2.71

1435375_at
BC052328
BM224662
2.71

1454650_at
Trim35
BQ175280
2.71

1455437_at
9030204A07Rik
AV369969
2.71

1427390_at
Bloc1s3
BC025913
2.71

1422595_s_at
5730470L24Rik
BC004090
2.70

1437801_at
Morf41l
BM115860
2.70

1417088_at
Zfp346
AW493043
2.70

1426316_at
6330416G13Rik
AV326978
2.70

1448223_at
Fto
NM_011936
2.70

1432158_a_at
Trappc2
AK007951
2.70

1429238_a_at
Ogfod2
AK004916
2.70

1418236_s_at
Atg5
AV168389
2.70

1450742_at
Bysl
BG079188
2.70

1433756_at
S100pbp
BI452674
2.70

1428609_at
Mylc2b
AW494458
2.70

1454605_a_at
Pi4k2a
BB208212
2.70

1448530_at
Gmpr
NM_025508
2.70

1457635_s_at
Nr3c1
BB096079
2.70

1447326_s_at
Zmym3
AW122925
2.70

1422725_at
Mak
BG069426
2.70

1451914_a_at
Add2
AF100423
2.70

1419169_at
Mapk6
BC024684
2.70

1420634_a_at
Smad2
NM_010754
2.69

1423090_x_at
Slc37a2
AV216331
2.69

1418865_at
Zfp385
NM_013866
2.69

1448195_at
Taf5l
NM_133966
2.69

1442829_at
Rin2
BB128602
2.69

1428695_at
9130227C08Rik
BB424872
2.69

1416101_a_at
Hist1h1c
NM_015786
2.69

1449089_at
Nrip1
NM_008735
2.69

1432329_a_at
Matk
AK018182
2.69

1427299_at
Rps6ka3
BE376079
2.69

1433914_at
AI747699
BM246193
2.69

1417506_at
Gmnn
NM_020567
2.69

1426577_a_at
1810054G18Rik
BM570079
2.69

1435425_at
Pisd
AV212090
2.69

1417463_a_at
2400001E08Rik
NM_025605
2.69

1440914_s_at
Aftph
BG071681
2.69

1451379_at
Rab22a
BC006596
2.69

1418417_at
Msc
NM_010827
2.69

1424503_at
Rab22a
BC006596
2.69

1424073_at
5430437P03Rik
BC005692
2.68

1448144_at
Hnrpab
NM_010448
2.68

1434646_s_at
Sap18
AV023865
2.68

1447865_x_at
Pdzd11
BB037788
2.68

1417959_at
Pdlim7
NM_026131
2.68

1441758_at
Rhot1
BB830916
2.68

1429931_at
4930553P18Rik
AK016112
2.68

1448320_at
Stim1
NM_009287
2.68

1418386_at
N6amt2
NM_026526
2.68

1459051_at
6530418L21Rik
BB781615
2.68

1438765_at
Dhx33
BB667230
2.68

1417196_s_at
Wwc2
NM_133791
2.68

1420841_at
Ptprf
BF235516
2.68

1457242_at
Ubox5
BB824671
2.68

1447682_x_at
Traf5
BB108799
2.68

1436300_at
Ripk5
BB435342
2.68

1428354_at
Foxk2
BM206907
2.68

1423799_at
Eif1
BI693609
2.68

1448637_at
Med25
NM_029365
2.68

1460447_at
Pus7l
AK019372
2.67

1434429_at
Syt16
AV348245
2.67

1457717_at
Anxa11
AI875033
2.67

1452742_at
Trak1
BB010301
2.67

1454767_at
D2Bwg1423e
BB359828
2.67

1422078_at
Akt3
AF124142
2.67

1436758_at
Hdac4
AI661423
2.67

1434744_at
Yrdc
BB230053
2.67

1434624_x_at
Rps9
AA762498
2.67

1447302_at

BE956835
2.67

1427732_s_at
Abcg4
BC026477
2.67

1417778_at
Zfp35
NM_011755
2.67

1437539_at
Prkaa1
BM236715
2.67

1460572_a_at
Zfp511
BQ178976
2.67

1451276_at
E030041M21Rik
BG066858
2.67

1416781_at
Praf2
NM_138602
2.67

1427271_at
Zbtb44
BC027138
2.67

1453631_at
Stx8
AK011376
2.67

1435618_at
Pnma2
BB473446
2.67

1446486_at
Dtwd2
BB068132
2.66

1428627_at
Zfp509
AK015819
2.66

1457426_at
1700048O20Rik
BE980678
2.66

1454835_at
Epm2aip1
AV340515
2.66

1428886_at
Rbbp5
AK016773
2.66

1460223_a_at
Epb4.9
NM_013514
2.66

1436633_at
LOC667668
BB323723
2.66

1417215_at
Rab27b
BB121269
2.66

1418895_at
Skap2
NM_018773
2.66

1426972_at
Sec24d
AK009425
2.66

1459806_x_at
Mrps23
BB110309
2.66

1449108_at
Fdx1
D43690
2.66

1431076_at
Add2
BG976853
2.66

1452846_at
Ppfia4
AK003571
2.65

1449913_at
Zfp2
NM_009550
2.65

1450184_s_at
Tef
NM_017376
2.65

1417479_at
Ppp2r3c
AV367559
2.65

1415952_at
Mark2
BI686265
2.65

1450668_s_at
Hspe1
NM_008303
2.65

1450436_s_at
Dnajb5
AI664344
2.65

1429832_at
Ppih
AK014665
2.65

1423294_at
Mest
AW555393
2.65

1442441_at
D11Bwg0434e
AV008016
2.65

1423302_a_at
Paxip1
AW742928
2.65

1434415_at
Dact3
BB797871
2.65

1425850_a_at
Nek6
BC019524
2.65

1455215_at
C530028O21Rik
AU067746
2.65

1427870_x_at
Igh-6
K00686
2.65

1439776_at
Ogfod1
BE956398
2.65

1424474_a_at
Camkk2
BI157430
2.65

1423160_at
Spred1
BQ044290
2.65

1444502_at
Pet112l
BG068580
2.64

1460634_at
Ralgds
NM_009058
2.64

1437927_at
Dlg2
AV343743
2.64

1453125_at
Sox11
BM508495
2.64

1421619_at
Kcnh3
NM_010601
2.64

1451273_x_at
BC025546
BC025546
2.64

1427113_s_at
Ttl
AV127581
2.64

1438201_at
Ppm2c
AV290622
2.64

1453069_at
Pik3cb
BF018155
2.64

1455463_at
Phyhip
BB427286
2.64

1448171_at
Siah2
AA414485
2.64

1441727_s_at
Zfp467
BE628275
2.64

1428800_a_at
Pus7l
AK019372
2.64

1423415_at
Gpr83
BB110067
2.64

1416514_a_at
Fscn1
NM_007984
2.64

1460576_at
Exoc6
AV248277
2.64

1426897_at
Rcc2
AV122997
2.64

1426307_at
Cyb5r4
BC025438
2.63

1450972_at
3110040N11Rik
AK019261
2.63

1429278_at
Nubpl
AK010827
2.63

1444470_x_at
Bmp5
AW556834
2.63

1435669_at
Zfp532
BB404446
2.63

1426548_a_at
Atpbd4
BG070516
2.63

1451334_at
1810009O10Rik
BC027022
2.63

1457080_at

BB046159
2.63

1456329_at
Prtg
BB130087
2.63

1417602_at
Per2
AF035830
2.63

1447403_a_at
Zmynd19
AW125726
2.63

1438922_x_at
Slc25a5
AV110784
2.63

1452025_a_at
Zfp2
AF483521
2.63

1448630_a_at
Sdhc
NM_025321
2.63

1456645_at
Wdr25
BB352668
2.63

1421820_a_at
Nf2
L28176
2.63

1420142_s_at
Pa2g4
AA672939
2.63

1455700_at
Mterfd3
BB359043
2.63

1434602_at
Thrap2
BG074645
2.63

1417016_at
Mapkapk5
NM_010765
2.63

1432489_a_at
2410187C16Rik
AK010831
2.62

1460498_a_at
Dnajc5
BI685030
2.62

1450847_at
Ncbp2
BE285362
2.62

1415753_at
D10Bwg1364e
BC005632
2.62

1437035_x_at
Rnf14
BB504639
2.62

1434762_at
Tmem142b
BF457736
2.62

1451745_a_at
Znhit1
BC026751
2.62

1457977_at
2310005N01Rik
AI594274
2.62

1440479_at
Cbx4
BB821623
2.62

1431836_x_at
5430432N15Rik
AK017382
2.62

1441387_at
E130309D02Rik
BB182811
2.62

1423756_s_at
Igfbp4
BC019836
2.62

1427901_at
Mrps18c
AK004139
2.62

1425656_a_at
Baiap2
AF390178
2.62

1435210_s_at
Snph
BQ174936
2.62

1425304_s_at
Prima1
AY043275
2.62

1430127_a_at
Ccnd2
AK007904
2.62

1433833_at
Fndc3b
BG064539
2.62

1452748_at
Xrcc3
AW537713
2.62

1419522_at
Zmynd19
NM_026021
2.62

1451080_at
Usp1
BC018179
2.62

1439405_x_at
Becn1
AV296285
2.62

1416247_at
Dctn3
NM_016890
2.62

1419987_at

C80388
2.62

1416938_at
Chchd1
NM_025366
2.62

1426403_at
Actr1b
BG801851
2.62

1433733_a_at
Cry1
BG069864
2.61

1455282_x_at
Alas1
AI255644
2.61

1418591_at
Dnaja4
NM_021422
2.61

1456785_at
Crsp2
BQ175889
2.61

1435844_at
A330009N23Rik
BB164247
2.61

1441815_at
AI851453
AI851453
2.61

1427910_at
Cst6
AK003744
2.61

1417507_at
Cyb561
BC006732
2.61

1455944_at
Zfp516
BB467812
2.61

1451444_s_at
2700008B19Rik
BF150550
2.61

1451141_at
Mettl8
BC004636
2.61

1427590_at
Zfp39
BC011182
2.61

1415925_a_at
Nup62
NM_053074
2.61

1433747_at
Lnpep
BQ176475
2.61

1427966_at
BC087945
AW551849
2.61

1448269_a_at
Klhl13
NM_026167
2.61

1433593_at
Ypel5
BB316456
2.61

1423733_a_at
Fiz1
AF126746
2.61

1449339_at
D10Ertd641e
BE692118
2.60

1452979_at
2610110G12Rik
AK014265
2.60

1436102_at
Sec22c
BB425408
2.60

1428259_at
Pxdn
AK010185
2.60

1456764_at
Slc35f3
BB427860
2.60

1418820_s_at
Zcchc10
BC025078
2.60

1448822_at
Psmb6
BC013897
2.60

1438481_at
AW047464
AI854375
2.60

1435843_x_at
Mrps9
AI666552
2.60

1433616_a_at
2310028O11Rik
BG793007
2.60

1430128_a_at
Reep6
AK002562
2.60

1427898_at
Rnf6
BI738010
2.60

1451294_s_at
Snrpe
BC008262
2.60

1448179_at
Usmg5
BC024355
2.60

1460444_at
Arrb1
AK004614
2.60

1424358_at
Ube2e2
BC016265
2.60

1442060_at
Prepl
BB224442
2.60

1439630_x_at
Sbsn
AI844734
2.60

1441610_at
N28178
BB306826
2.60

1432232_at
Rcor3
AK016486
2.60

1455356_at
Camsap1
BG064669
2.59

1429837_at
1700016D18Rik
AV278908
2.59

1422616_s_at
Wdr54
NM_023790
2.59

1442793_s_at
Tbrg4
BB139935
2.59

1456737_x_at
Acaa1a
BB314154
2.59

1420612_s_at
Ptp4a2
BE134116
2.59

1452357_at
4-Sep
AF033350
2.59

1460462_at
Med18
AK012903
2.59

1429590_at
Tacc1
BE862546
2.59

1416796_at
Nck2
NM_010879
2.59

1448682_at
Dynll1
NM_019682
2.59

1449194_at
Mrps25
AK004037
2.59

1429170_a_at
Mtf1
AK012676
2.59

1435602_at

BE200310
2.59

1422264_s_at
Klf9
NM_010638
2.59

1450535_at
Krtap12-1
NM_010670
2.59

1455672_s_at
Cplx2
BE946238
2.59

1416301_a_at
Ebf1
BB125261
2.59

1427173_a_at
Mrps33
Y17852
2.59

1456765_at
6430511F03
AV339861
2.59

1449258_at
D11Wsu99e
AV225714
2.59

1444441_at
Rapgefl1
AW490909
2.59

1425147_at
2410075B13Rik
BC024536
2.58

1420858_at
Pkia
AK010212
2.58

1437959_at
Zfp324
BB277175
2.58

1443782_x_at
Cyp20a1
AV214523
2.58

1451217_a_at
Immp1l
BC008259
2.58

1443427_at

C76966
2.58

1439834_at
2400009B08Rik
AA261399
2.58

1460377_a_at
Tmem8
BC005722
2.58

1455104_at
Mxd1
AV228517
2.58

1452604_at
Stard13
BB667840
2.58

1441786_at

AW048005
2.58

1436876_at
Rgs7bp
AV337421
2.58

1449188_at
Midn
NM_021565
2.58

1435289_at
D230014K01Rik
BB379430
2.58

1417628_at
Supt6h
NM_009297
2.58

1417300_at
Smpdl3b
NM_133888
2.58

1428289_at
2310051E17Rik
AW488885
2.58

1428049_a_at
Nudt16l1
BC028494
2.58

1422297_at
Pfdn5
NM_027044
2.58

1460315_s_at
Tbk1
NM_019786
2.58

1420866_at
Zfp161
NM_009547
2.57

1449770_x_at
D16Bwg1494e
N28171
2.57

1455877_a_at
Nanos1
BG075296
2.57

1449666_at
Atrnl1
AW555641
2.57

1452101_at
Blmh
BB215260
2.57

1416323_at
Kctd20
AV281062
2.57

1435392_at
Wdr17
BB277182
2.57

1426487_a_at
Rbbp6
BB092954
2.57

1423652_at
Isca1
AV209097
2.57

1421557_x_at
Txn2
NM_019913
2.57

1453834_at
Ssu72
BB857377
2.57

1452988_at
2610306M01Rik
AK011997
2.57

1416699_at
1110008F13Rik
NM_026124
2.57

1423102_a_at
Rnf10
AI746548
2.57

1440478_at
D10Ertd438e
BM941145
2.57

1427914_a_at
Tceb1
AI019214
2.57

1454985_at
D030051N19Rik
BI872009
2.57

1452516_at
Smok3a
AJ245454
2.57

1427956_at
Pcgf1
BG068910
2.57

1423651_at
Isca1
AV209097
2.56

1416155_at
Hmgb3
NM_008253
2.56

1460563_at
D10Ertd438e
BE949414
2.56

1448262_at
Psmb2
NM_011970
2.56

1431838_at
1700128E19Rik
AK007309
2.56

1437185_s_at
Tmsb10
AV148480
2.56

1438422_at
Lrrc20
BB143476
2.56

1423456_at
Bzw2
BM932775
2.56

1424095_at
Rtcd1
BC016519
2.56

1453175_at
Zbtb25
AK013374
2.56

1423568_at
Psma7
BG297088
2.56

1448505_at
C1d
NM_020558
2.56

1428619_at
2310005N03Rik
BB783243
2.56

1423588_at
Arpc4
BG145444
2.55

1421630_at
Zfy1
NM_009570
2.55

1427124_at
4932416H05Rik
AB041802
2.55

1426810_at
Jmjd1a
BC026605
2.55

1449076_x_at
Adi1
NM_134052
2.55

1427060_at
Mapk3
BI414398
2.55

1448882_at
Tmem93
NM_025318
2.55

1438689_at
4632433K11Rik
BB430846
2.55

1421910_at
Tcf20
AW552808
2.55

1426966_at
Axin1
BB004060
2.55

1453689_at
Fance
AK013325
2.55

1452002_at
Zfp508
BC024969
2.55

1418121_at
Vrk3
NM_133945
2.55

1451595_a_at
Kcnq2
AB000497
2.55

1440376_at
Fbxo41
AI747062
2.55

1422998_a_at
Glrx2
NM_023505
2.55

1426313_at
Bre
BE984258
2.55

1421342_at
Kcns2
NM_008436
2.55

1418732_s_at
1500041N16Rik
NM_026399
2.54

1428913_at
D2Bwg1335e
AK004089
2.54

1428413_at
Ccny
AK017493
2.54

1422451_at
Mrps21
NM_078479
2.54

1436955_at
Tssc1
BG065248
2.54

1424240_at
Arfip2
BC022942
2.54

1422320_x_at
Phxr5
NM_008836
2.54

1454846_at
Utp15
AV227804
2.54

1449303_at
Sesn3
NM_030261
2.54

1424044_at
Jmjd2b
BC007145
2.54

1416127_a_at
Dnpep
NM_016878
2.54

1418112_at
Mrpl10
BC016219
2.54

1435019_at
Atxn713
BB532141
2.54

1417279_at
Itpr1
NM_010585
2.54

1452724_at
Ppp1r16a
AK013817
2.54

1434228_at
Ppm2c
AV255921
2.54

1424289_at
Osgin2
BB817847
2.54

1416123_at
Ccnd2
NM_009829
2.54

1434420_x_at
Tomm22
AV102576
2.54

1437148_at
Arpc2
BG967632
2.54

1436134_at
Scn2b
BQ175340
2.54

1439022_at
Phactr1
AV259240
2.54

1428603_at
Glcci1
AK009885
2.54

1436567_a_at
Ndufa7
C88880
2.53

1450484_a_at
Tyki
AK004595
2.53

1432509_at
5033430I15Rik
AK017206
2.53

1431020_a_at
Fgfr1op2
AK008983
2.53

1423834_s_at
Gga1
BC026802
2.53

1435960_at
EG434128
BI733057
2.53

1416507_at
Tmem4
NM_019953
2.53

1454987_a_at
H2-Ke6
AI323545
2.53

1417774_at
Nans
BI151886
2.53

1454829_at
Rundc1
BB796494
2.53

1452201_at
2310047B19Rik
BC019749
2.53

1427006_at
Rapgef1
BB339051
2.53

1428528_at
1110007L15Rik
BM124553
2.53

1426586_at
Slc25a11
AI647500
2.53

1452669_at
2810012G03Rik
AK012724
2.53

1452024_a_at
Ldb1
AF030333
2.53

1449326_x_at
Saa2
NM_011314
2.53

1434381_at
BC060631
AV287602
2.53

1428972_at
0610012D17Rik
AK007178
2.53

1428515_at
2410012H22Rik
AK010472
2.53

1432415_at
Rab3c
AK014050
2.53

1433460_at
Ttc7b
BB329157
2.53

1460617_s_at
Rab6b
AV220161
2.52

1424236_at
Tbc1d10b
AF285112
2.52

1453737_at
Wipf2
BI157060
2.52

1449003_a_at
Vti1b
NM_016800
2.52

1421992_a_at
Igfbp4
NM_010517
2.52

1433682_at
Arhgef17
BE287052
2.52

1426716_at
Tdrd7
BC025099
2.52

1425650_at
Tle4
AF229633
2.52

1455982_at
Jmjd4
BF466891
2.52

1449281_at
Nrtn
NM_008738
2.52

1444531_at
Sod2
AI847438
2.52

1426514_at
4631426J05Rik
AK019474
2.52

1418827_at
Thex1
BF320755
2.52

1422693_a_at
Sub1
BE989104
2.52

1457675_at
2510002D24Rik
BG063089
2.52

1453030_at
Msl2l1
BB745314
2.52

1435912_at
Ubxd7
BM220232
2.52

1428447_at
Tmem14a
AK017734
2.52

1429290_at
Cbx6
BE951971
2.52

1451321_a_at
Rbm43
BC003333
2.52

1416535_at
Mcrs1
BC003746
2.52

1449122_at
3110003A22Rik
NM_026534
2.52

1447683_x_at
Mettl1
AV118676
2.52

1452048_at
Mrpl12
AK002757
2.51

1423502_at
Brd2
BI155271
2.51

1428141_at
Gga2
AK004632
2.51

1428508_at
Tbc1d2b
AK004526
2.51

1434832_at
Foxo3a
BB364488
2.51

1422164_at
Pou3f4
X66603
2.51

1451672_at
Gprk6
AF040748
2.51

1458979_at

BB332816
2.51

1429043_at
Smndc1
BG071121
2.51

1428519_at
2610528E23Rik
AK019979
2.51

1416850_s_at
Zcd1
NM_134007
2.51

1434834_at
Socs7
BG067098
2.51

1452380_at
Epha7
BB075797
2.51

1420860_at
Itga9
NM_133721
2.51

1457250_x_at
Nr1d2
BB147330
2.51

1419180_at
Bcl9l
NM_030256
2.51

1426997_at
Thra
AW208371
2.51

1431378_at
Nr3c1
BB600983
2.51

1451872_a_at
Neurl
AF400063
2.50

1418915_at
Mmachc
NM_025962
2.50

1416110_at
Slc35a4
NM_026404
2.50

1450061_at
Enc1
BM120053
2.50

1448411_at
Wfs1
NM_011716
2.50

1422558_at
Gamt
AF015887
2.50

1441178_at
Dtwd2
BB818617
2.50

1424136_a_at
Ppih
BC016565
2.50

1443133_at
Nefm
BB394014
2.50

1420472_at
Mtpn
NM_008098
2.50

1430979_a_at
Prdx2
AK011963
2.50

1428531_at
Ints7
BB457797
2.50

1427063_at
5330417C22Rik
AK017241
2.50

1435902_at
Nudt18
BM120193
2.50

1424049_at
Lrrc42
BC027203
2.50

1439541_at
Mat2a
BQ176011
2.50

1455640_a_at
Txn2
AV053127
2.50

1444981_at
Punc
BG067286
2.50

1452921_at
B130050I23Rik
AK006992
2.50

1437571_at
Hic2
BB292220
2.50

1452972_at
Ttc32
AK005950
2.50

1415793_at
Pnpo
NM_134021
2.50

1438387_x_at
Top3b
BB107606
2.50

1424538_at
Ubl4
BI650739
2.50

1426381_at
Pprc1
BM199989
2.50

1460307_at
Akt3
AF124142
2.50

1416327_at
Ufc1
NM_025388
2.49

1423636_at
Wdr31
AK017259
2.49

1451140_s_at
Prkag2
BB756794
2.49

1426582_at
Atf2
BM119623
2.49

1439837_at
Tnrc15
BE136147
2.49

1423448_at
Rab11b
BE986863
2.49

1453020_at
1810048J11Rik
AK007829
2.49

1455328_at
Accn2
BQ176072
2.49

1452328_s_at
Pja2
BF160731
2.49

1419671_a_at
Il17rc
NM_134159
2.49

1454669_at
Tmem11
BG075225
2.49

1438274_at
Ikzf4
BG071647
2.49

1420579_s_at
Cftr
NM_021050
2.49

1448326_a_at
Crabp1
NM_013496
2.48

1436994_a_at
Hist1h1c
BB533903
2.48

1416863_at
Abhd8
NM_022419
2.48

1448703_at
Lsm8
NM_133939
2.48

1448784_at
Taf10
NM_020024
2.48

1434900_at
Mkl1
BM196656
2.48

1434843_at
A430041B07Rik
BG070968
2.48

1419766_at
Snf1lk
NM_010831
2.48

1416882_at
Rgs10
NM_026418
2.48

1436059_at
Rfx1
BB151173
2.48

1431387_at

AK015329
2.48

1425511_at
Mark1
BM213279
2.48

1427496_at
Cep152
BC026366
2.48

1455969_at
Nudt15
BB198496
2.48

1454018_at
Tlk2
AK014829
2.48

1455039_a_at
Sin3b
BF017589
2.48

1423371_at
Pole4
BF577544
2.48

1425884_at
Bxdc1
BC025093
2.48

1454874_at
Btbd7
BG076293
2.48

1453561_x_at
Prkg1
BB809910
2.48

1455489_at
Lrrtm2
BB281991
2.48

1430679_at
4933404O12Rik
AK016656
2.48

1431334_a_at
4933433P14Rik
AK017049
2.48

1434410_at
Mina
AV276428
2.47

1452343_at
D18Ertd653e
BQ032363
2.47

1455211_a_at
Timm13
BF021416
2.47

1432411_a_at
Fbxw2
AK009893
2.47

1437424_at
Syde2
BG069296
2.47

1422568_at
Ndel1
AF323918
2.47

1428235_at
Sdhd
AK013962
2.47

1428473_at
Ppp3cb
AK004360
2.47

1452989_at
2900009J20Rik
BB315961
2.47

1419834_x_at
Mark1
AW491150
2.47

1442180_at
Dleu7
BB382040
2.47

1434029_at
2410025L10Rik
AW545676
2.47

1433744_at
Lrtm2
BB180412
2.47

1416948_at
Mrpl23
NM_011288
2.47

1435636_at
2310051F07Rik
AI415636
2.47

1454807_a_at
Snx12
BB414983
2.47

1423478_at
Prkcb1
BF660388
2.47

1437606_at

BB829614
2.46

1434637_x_at
Sin3b
BF017589
2.46

1435253_at
Rab11b
BM250605
2.46

1423715_a_at
Nedd8
BC004625
2.46

1452467_at
Mmab
BQ042988
2.46

1436402_at
Dohh
BG292769
2.46

1427233_at
Tshz1
AV291373
2.46

1454851_at
Nr2c2
AV162817
2.46

1432091_a_at
Rutbc3
AK007455
2.46

1433523_at
D930005D10Rik
BB521978
2.46

1417230_at
Ralgps2
NM_023884
2.46

1440177_at
9.63E+17
BM899529
2.46

1428216_s_at
Tomm7
BB609428
2.46

1435675_at
Tbc1d12
BF228251
2.46

1416706_at
Rpe
BG916066
2.46

1439515_at
Setd5
BM207556
2.46

1434556_at

BI437942
2.46

1448219_a_at
Ywhaz
AV027921
2.46

1431712_a_at
2310022A10Rik
AK009941
2.46

1452039_a_at
Bap1
AK009033
2.46

1439371_x_at
Timm44
AV102008
2.45

1429033_at
Gcc1
AV339946
2.45

1422714_at
Ube2i
U31934
2.45

1424380_at
Vps37b
BC026744
2.45

1426888_at
Ehmt2
BI412952
2.45

1422418_s_at
Supt4h2
NM_011509
2.45

1449080_at
Hdac2
NM_008229
2.45

1424940_s_at
BC022687
BC022687
2.45

1428457_at
5830472M02Rik
AK014262
2.45

1455191_x_at
Pip5k1b
BB822856
2.45

1424624_at
2900011O08Rik
BC022741
2.45

1451187_at
0610037P05Rik
BC011300
2.45

1438917_x_at
Nup62
AW240611
2.45

1440384_at
Nfkbiz
AI506779
2.45

1453007_at
3110082I17Rik
AK014271
2.45

1423625_a_at
Dnajc19
AV053772
2.45

1452791_at
Coq2
AK009092
2.45

1422480_at
Snx3
NM_017472
2.45

1433924_at
Peg3
BM200248
2.45

1417239_at
Cetn3
BC002162
2.45

1426112_a_at
Cd72
BC003824
2.44

1447540_at
Tigd3
AV167924
2.44

1417241_at
X83328
NM_025275
2.44

1415686_at
Rab14
AV339290
2.44

1448382_at
Ehhadh
NM_023737
2.44

1422473_at
Pde4b
BM246564
2.44

1421529_a_at
Txnrd1
NM_015762
2.44

1441738_at

BE824623
2.44

1424379_at
Car11
BC019393
2.44

1434908_at
AI480556
BB212188
2.44

1420707_a_at
Traip
AK012948
2.44

1455279_at
Gm1060
BG070552
2.44

1452226_at
Rcc2
AV122997
2.44

1428316_a_at
Fundc2
AI510221
2.44

1441974_at
Camk4
BB209139
2.44

1447653_x_at
Rpl24
AV153119
2.44

1452313_at
5930416I19Rik
AK011167
2.44

1426769_s_at
Maml1
BB522516
2.44

1445479_at
Ttbk1
AI839084
2.44

1439180_at
A430093A21Rik
BE197105
2.44

1431420_s_at
Nsd1
BI082843
2.44

1455665_at
Lonrf1
BB705689
2.44

1419406_a_at
Bcl11a
NM_016707
2.44

1420488_at
Mrps14
BI246587
2.43

1435747_at
Fgf14
AV141013
2.43

1449935_a_at
Dnaja3
AK004575
2.43

1451808_at
Kcnj4
U11075
2.43

1456767_at
Lrfn3
AV062156
2.43

1428797_at
Setd6
AK010304
2.43

1437049_at

BE627361
2.43

1452860_at
Fbxl17
AK004419
2.43

1454805_at
Wtap
AV141160
2.43

1435039_a_at
Pip5k1b
BB822856
2.43

1425132_at
Neto1
AF448840
2.43

1448543_at
Slmo2
NM_025531
2.43

1418234_s_at
Bcas2
NM_026602
2.43

1423910_at
Centg3
AF459091
2.43

1428097_at
2510009E07Rik
AK010940
2.43

1434439_at
Gsk3b
BQ175580
2.43

1438199_at
AI316807
AW228053
2.43

1436763_a_at
Klf9
AI267126
2.43

1452833_at
Rapgef2
AK018008
2.43

1426012_a_at
2610301G19Rik
BC010499
2.43

1429792_at
9530048O09Rik
BB398793
2.43

1423843_at
Lrrc61
BC027309
2.43

1416853_at
Ncdn
BC017126
2.43

1438300_at
Cdkn2aip
BB371618
2.42

1444914_at

AW121574
2.42

1429239_a_at
Stard4
AK014587
2.42

1440386_at
Glce
BB272589
2.42

1454640_at
Chchd7
BI692256
2.42

1460623_at
Skap2
BB753881
2.42

1450942_at
Ccdc16
AI530149
2.42

1425052_at
Isoc1
AK010892
2.42

1431538_at
4931415C17Rik
AV271801
2.42

1428522_at
Ttf2
BB283807
2.42

1422938_at
Bcl2
NM_009741
2.42

1427039_at
Epn1
AF057285
2.42

1424267_at
1810043G02Rik
BC010330
2.42

1435755_at
1110001A16Rik
BB609468
2.42

1460337_at
Sh3kbp1
BB326929
2.42

1452922_at
Ppp1r3d
AK011539
2.42

1419267_at
Nfyb
AV250496
2.42

1455327_at
Senp2
BI684973
2.42

1453720_at
Rnf157
AK011693
2.42

1454695_at
Wdr18
BQ176396
2.42

1416135_at
Apex1
NM_009687
2.42

1435237_at
2310009A05Rik
AV004950
2.42

1455756_at
F730014I05Rik
BB232864
2.42

1454609_x_at
6430527G18Rik
BB770958
2.42

1423971_at
Thoc3
BE335845
2.41

1458951_at
Vrk1
AV341598
2.41

1436380_at
Cdc42bpa
BG518726
2.41

1417542_at
Rps6ka2
BB737182
2.41

1422718_at
Ap3s2
NM_009682
2.41

1455546_s_at
Sf3a2
BI900579
2.41

1451170_s_at
Nomo1
BC024503
2.41

1429071_at
Me3
AK006146
2.41

1426843_at
1810013C15Rik
BG065569
2.41

1453013_at
Zfp740
AW548228
2.41

1438017_at
Rusc1
AV327629
2.41

1457979_at

BM938335
2.41

1423189_at
6720456B07Rik
BI456243
2.41

1459386_at
Zfp709
AV319015
2.41

1422919_at
Hrasls
NM_013751
2.41

1424293_s_at
Tmem55a
BC021435
2.41

1455637_x_at
EG245305
BB465277
2.41

1424510_at
Nudt6
BC027267
2.41

1434853_x_at
Mkrn1
AA717142
2.41

1450082_s_at
Etv5
BG966751
2.41

1460636_at
Map2k2
AW553456
2.40

1428466_at
Chd3
AK011183
2.40

1455075_at
Pigv
BB321057
2.40

1417286_at
Ndufa5
NM_026614
2.40

1454963_at
E430028B21Rik
BQ127172
2.40

1425192_at
Klhl25
BC027373
2.40

1430137_at
Map3k13
AK004850
2.40

1419189_at
Vti1a
BC019386
2.40

1435362_at
Foxj3
BB026073
2.40

1425071_s_at
Ntrk3
BE692701
2.40

1429509_at
Lsm12
BB771548
2.40

1455025_at
Paqr9
AV103696
2.40

1419138_at
B3galt4
NM_019420
2.40

1435556_at
Zfp597
AV270881
2.40

1455362_at
Angel2
AV275390
2.40

1424520_at
2010305A19Rik
BC012391
2.40

1450638_at
Pdcd5
AF161074
2.40

1426727_s_at
Ppp1r10
BC004771
2.40

1427895_at
2310004N24Rik
AK009156
2.40

1452797_at
Fastkd3
AK009264
2.40

1423446_at
Dapk3
AI642212
2.40

1443948_at
Ttc9c
BB771921
2.40

1429244_at
1500011B03Rik
AW494389
2.40

1449186_at
Bag4
NM_026121
2.40

1427413_a_at
Cugbp1
AK014492
2.40

1449855_s_at
Uchl3
AB033370
2.40

1452254_at
Mtmr9
BB292990
2.39

1448483_a_at
Ndufb2
NM_026612
2.39

1440364_a_at
A230062G08Rik
BB760971
2.39

1451347_at
Ccdc95
BG065288
2.39

1422798_at
Cntnap2
AU079588
2.39

1425180_at
Sgip1
AV344708
2.39

1455671_at
Commd8
AV365904
2.39

1437239_x_at
Phc2
BB334118
2.39

1427158_at
Mrps30
AV095945
2.39

1433867_at
1810030O07Rik
BG064658
2.39

1451118_a_at
2410018C17Rik
BC016596
2.39

1429484_at
Kif3c
BB752416
2.39

1457279_at

BB314393
2.39

1451272_a_at
Ube2f
BC016117
2.39

1450472_s_at
Smad3
BI150236
2.39

1448664_a_at
Speg
NM_007463
2.39

1434309_at
Fntb
BG063077
2.39

1416537_at
Creld1
NM_133930
2.39

1437848_x_at
Adpgk
BB490673
2.39

1454637_at
Klhl8
BM942651
2.39

1422716_a_at
Acp1
AW554436
2.39

1450054_at
Add1
BF140063
2.39

1419967_at
Seh11
AW540070
2.39

1447320_x_at
Rpo1-3
AU018636
2.39

1425940_a_at
Ssbp3
AF170906
2.39

1437477_at
Lrrfip1
AV301584
2.39

1435802_at
ZBTB45
BE134048
2.38

1441453_at
Dennd2a
BB341122
2.38

1420191_s_at
D16Bwg1494e
N28171
2.38

1454806_at
D12Ertd553e
AV228737
2.38

1455497_at
Leng9
BB799608
2.38

1418529_at
Osgep
NM_133676
2.38

1435762_at
Pacs1
BM115617
2.38

1424150_at
Gdpd5
BC024955
2.38

1417138_s_at
Polr2e
NM_025554
2.38

1450547_x_at
Dub2
U70369
2.38

1424216_a_at
Papola
U52197
2.38

1453093_at
Rasgef1c
AK018593
2.38

1416774_at
Wee1
NM_009516
2.38

1435598_at

BE996371
2.38

1430229_at
6330416L11Rik
AK018183
2.38

1436077_a_at
Fcho1
BF730694
2.38

1428127_at
4921506J03Rik
BG975608
2.38

1423138_at
Wdr4
BE854862
2.38

1452873_at
5830415F09Rik
AK017931
2.38

1424554_at
Ppp1r8
BC025479
2.38

1435683_a_at
Abcc5
AV150520
2.38

1423408_a_at
2500003M10Rik
BE692070
2.38

1434423_at
Gulp1
BB138485
2.38

1443058_at
2900006F19Rik
AV242558
2.38

1450943_at
2010012C16Rik
AK006303
2.38

1433462_a_at
Pi4k2a
BB208212
2.37

1417974_at
Kpna4
BF018653
2.37

1427326_at
4833439L19Rik
BB489450
2.37

1418032_at
Itfg2
NM_133927
2.37

1417794_at
Zmym3
NM_019831
2.37

1424197_s_at
Fance
BC016538
2.37

1436053_at
Tbc1d22b
BB272520
2.37

1418085_at
Prkcz
NM_008860
2.37

1426344_at
Gle11
AK016671
2.37

1460441_at
Zxdb
BF660409
2.37

1433969_at
AU067824
AU067824
2.37

1448582_at
Ctnnbl1
NM_025680
2.37

1452657_at
Ap1s2
AK005223
2.37

1426811_at
Ppp2r5b
BB080065
2.37

1448202_x_at
2610524G07Rik
NM_025596
2.37

1417339_a_at
Dynll1
NM_019682
2.37

1427929_a_at
Pdxk
BG063905
2.37

1447725_at
C030034E14Rik
BE948505
2.37

1448542_at
Bccip
NM_025392
2.37

1435866_s_at
Hist3h2a
AV297651
2.37

1421196_at
Ptpn11
NM_011202
2.36

1455198_a_at
Ppp2r3a
BB550312
2.36

1418619_at
Icam5
NM_008319
2.36

1417612_at
Ier5
BF147705
2.36

1417941_at
Nanp
NM_026086
2.36

1435187_at
Tomm20
BG064946
2.36

1440229_at
2310034G01Rik
AI854627
2.36

1448817_at
Otub1
NM_134150
2.36

1452918_at
D19Ertd737e
AK011404
2.36

1455032_at
Ccnyl1
BQ176303
2.36

1436129_at
4930523C11Rik
AV255799
2.36

1428395_at
Smurf1
BB832916
2.36

1428991_at
Hrasls
BQ176967
2.36

1446957_s_at
BC004022
C81621
2.36

1460274_at
4921530G04Rik
NM_027622
2.36

1424052_at
Thap4
BC013538
2.36

1428937_at
Atp2b1
BI080417
2.36

1437624_x_at
Nudt16l1
AV211435
2.36

1454869_at
Wdr40b
BB274776
2.35

1451300_a_at
Chmp7
BC024115
2.35

1435588_at
Wdfy1
BQ031098
2.35

1416267_at
Scoc
NM_019708
2.35

1441855_x_at
Cxcl1
BB554288
2.35

1451726_at
Mtmr6
BC020019
2.35

1423984_a_at
Mrg2
BC003762
2.35

1423899_at
Trip12
BG923744
2.35

1451436_at
Sbno1
BC023136
2.35

1418319_at
1810047C23Rik
NM_138668
2.35

1423113_a_at
Ube2d3
AK009276
2.35

1448946_at
Kif3c
NM_008445
2.35

1427305_at
Piga
AV340508
2.35

1438016_at
BC068171
BG068512
2.35

1448181_at
Klf15
BC013486
2.35

1454944_at
Hic2
BE648070
2.35

1435231_at
Coq4
BB667890
2.35

1433528_at
Gtf2a2
BG075741
2.35

1424710_a_at
Gorasp2
BC016455
2.35

1453200_at
Rai1
AK013909
2.35

1459657_s_at
Rpo1-3
AU018636
2.35

1436523_s_at
1810022K09Rik
AV111033
2.35

1431429_a_at
Arl4a
AK006286
2.35

1454614_at
1810013D10Rik
C87702
2.34

1444980_at
Onecut2
BE996518
2.34

1457985_at
Clmn
BE980727
2.34

1456044_at
Nedd41
BB309512
2.34

1452208_at
Prdm4
AK013393
2.34

1445342_at
C230004F18Rik
AV338249
2.34

1428742_at
Fbxo45
AK011438
2.34

1425830_a_at
2810452K22Rik
BC021867
2.34

1422895_at
Vamp4
NM_016796
2.34

1437436_s_at
Gprk6
BB461269
2.34

1416877_a_at
Mrpl51
AI594880
2.34

1416963_at
Ubadc1
NM_133835
2.34

1422688_a_at
Nras
BB018528
2.34

1440228_at
Ranbp6
BB477637
2.34

1435685_x_at
Abcc5
AV150520
2.34

1420381_a_at
Rpl31
NM_053257
2.34

1426620_at
Chst10
BB549997
2.34

1428147_at
Coro7
BB203098
2.34

1449181_at
Fech
NM_007998
2.34

1455726_at
Gm71
BB756522
2.34

1436081_a_at
Zfp414
BE632205
2.34

1452216_at
Mcat
BB494388
2.34

1425481_at
Cnot6l
BC018506
2.33

1423032_at
Rpl39
AV107150
2.33

1451214_at
Kbtbd2
BC022962
2.33

1435883_at
Tmco7
BM206080
2.33

1436158_at
Eif4ebp2
BI453292
2.33

1456468_x_at
1110012N22Rik
BB189996
2.33

1423169_at
Taf7
AV213552
2.33

1437336_x_at
1110002E23Rik
AV051733
2.33

1429245_at
1810009N02Rik
BI202412
2.33

1435426_s_at
Pisd
AV212090
2.33

1433985_at
Abi2
AV263684
2.33

1416502_a_at
Preb
NM_016703
2.33

1447754_x_at
Thap4
BB130418
2.33

1452896_at
Gtl3
AK011217
2.33

1428527_at
Snx7
AK011015
2.33

1451398_at
Cbr4
BC009118
2.33

1416402_at
Abcb10
AV382118
2.33

1444661_at
Gpr26
BB247791
2.33

1423658_at
Sppl3
BC023131
2.33

1455243_at
Brpf3
BB728354
2.33

1421262_at
Lipg
BC020991
2.33

1424771_at
H2afj
BF661121
2.33

1455430_at
Prr8
BQ179705
2.33

1437335_x_at
Poldip3
BB534975
2.33

1440124_at
Ppap2b
BB312387
2.33

1427998_at
Lsm12
AV015526
2.33

1434970_a_at

AV216669
2.33

1429718_at
Slitrk5
BB782729
2.33

1425820_x_at
Gpatc4
BC019490
2.33

1438402_at
9630050M13Rik
BB131315
2.32

1423794_at
D2Ertd391e
BM231455
2.32

1423122_at
Avpi1
BI649826
2.32

1428718_at
Sern1
AW490544
2.32

1439755_at
Sipa1l1
BM936013
2.32

1429723_at
6330409N04Rik
AK018153
2.32

1452100_at
Dullard
AV162210
2.32

1417284_at
Mapkap1
NM_133841
2.32

1455143_at
Nlgn2
AU042744
2.32

1417080_a_at
Ecsit
NM_012029
2.32

1452680_at
Snrpd2
BQ043840
2.32

1427669_a_at
Cit
AF070066
2.32

1448517_at
Timm22
AK012130
2.32

1429759_at
Rps6ka6
BB449218
2.32

1448153_at
Cox5a
NM_007747
2.32

1434794_at
Rhof
BM241811
2.32

1456557_at
1700041C02Rik
BB256666
2.32

1439130_at
Slc44a2
BB131502
2.32

1444062_at
2900056L01Rik
AI428930
2.32

1441019_at
Fbxo3
BB012129
2.32

1450673_at
Col9a2
NM_007741
2.32

1436078_at
Fcho1
BF730694
2.31

1426786_s_at
Dhx38
BM195397
2.31

1448357_at
Snrpg
NM_026506
2.31

1426408_at
Cugbp1
BI412951
2.31

1458040_at
D7Wsu130e
BM213832
2.31

1440200_at
9630031F12Rik
BB128528
2.31

1436373_at
Map3k10
AA789425
2.31

1442789_at

AW125266
2.31

1418602_at
Cdh15
NM_007662
2.31

1458295_at
Tmem79
BE626145
2.31

1449172_a_at
Lin7b
NM_011698
2.31

1433826_at
Tspyl3
AV325152
2.31

1453705_at
B230110C06Rik
AK020961
2.31

1435061_at
Nudt10
AI853080
2.31

1449256_a_at
Rab11a
BC010722
2.31

1452899_at
Rian
AK017440
2.31

1416952_at
Atp6v1d
NM_023721
2.31

1459859_x_at
Chrac1
BB297047
2.31

1420809_a_at
1500003O03Rik
NM_019769
2.31

1429671_at
2410018M08Rik
AK010551
2.31

1426222_s_at
Loh11cr2a
BC004727
2.31

1455208_at
Pex19
AV229364
2.31

1455639_at
Slc25a39
AV224593
2.31

1431822_a_at
Azi2
AK004992
2.31

1451267_at
Sharpin
BC016203
2.31

1419787_a_at
Zfp628
AI608468
2.31

1433971_at
Camta1
BB558154
2.31

1424331_at
Rab40c
AF422144
2.30

1449017_at
Nutf2
AA920031
2.30

1452666_a_at
Tmcc2
AK004359
2.30

1416018_at
Dr1
NM_026106
2.30

1426240_at
Chmp4b
BC011429
2.30

1437366_at
AI608492
BB332542
2.30

1415940_at
Zfand2a
NM_133349
2.30

1450243_a_at
Dscr1l1
NM_030598
2.30

1448968_at
D7Wsu128e
BC011313
2.30

1431419_at
Nsd1
BI082843
2.30

1425507_at
Arfrp1
BG695431
2.30

1427427_at
Ryr3
AV238793
2.30

1428237_at
2700059D21Rik
BI689227
2.30

1442175_at
C030027H14Rik
BB358264
2.30

1430777_a_at
Golph3
AK014644
2.30

1438409_at
Cep63
BG061859
2.30

1453169_a_at
Gtf2h1
BE688673
2.30

1424296_at
Gclc
BC019374
2.30

1453570_x_at
Bet11
AV252862
2.30

1423823_at
Mrto4
BC005734
2.30

1422979_at
Suv39h2
NM_022724
2.30

1448131_at
Mfn2
NM_133201
2.29

1428524_at
Cars2
AK009937
2.29

1417195_at
Wwc2
NM_133791
2.29

1428025_s_at
Pitpnc1
BC028271
2.29

1423389_at
Smad7
BF226166
2.29

1446312_at

BB273243
2.29

1424539_at
Ubl4
BI650739
2.29

1438434_at
Arhgap11a
AV349116
2.29

1442763_s_at
Ttll10
AW324354
2.29

1418835_at
Phlda1
NM_009344
2.29

1416041_at
Sgk
NM_011361
2.29

1416742_at
Cfdp1
NM_011801
2.29

1460439_at
BC033915
AK017789
2.29

1438209_at
4632404H12Rik
BB216617
2.29

1435799_at
Sfrs14
BB772209
2.29

1456789_at
Zfp462
AW491540
2.29

1448293_at
Ebf1
BB125261
2.29

1423832_at
Prkag2
BB756794
2.29

1424620_at
D13Wsu177e
AK013251
2.29

1422972_s_at
Gcn5l2
NM_020004
2.29

1436268_at
Ddn
AI841578
2.29

1433873_s_at
Pcnt
BG069597
2.29

1417571_at
Mpg
BC014754
2.29

1439012_a_at
Dck
BB030204
2.29

1454012_a_at
Parp6
AK021076
2.28

1438091_a_at
H2afz
AV003424
2.28

1431068_at
Rmnd5a
BI659732
2.28

1456524_at
Nrg1
AV318914
2.28

1440603_at

BB073590
2.28

1427279_at
Clip4
AK017914
2.28

1442099_at
Usp31
BM227490
2.28

1451021_a_at
Klf5
BI465857
2.28

1453369_a_at
Fundc1
AK007686
2.28

1423833_a_at
Brp44
BC018324
2.28

1423534_at
Pdcd2
BI526195
2.28

1425929_a_at
Rnf14
AF249668
2.28

1435695_a_at
A030007L17Rik
AA673177
2.28

1421604_a_at
Klf3
NM_008453
2.28

1427501_at
BC007180
BG083894
2.28

1454034_a_at
Usp21
AK013252
2.28

1455023_at
N28178
BB171181
2.28

1454749_at
Pcnt
BG069597
2.28

1456795_at
D330027G24Rik
BB449568
2.28

1451724_at
Ankmy2
BC024959
2.28

1436484_at
C030019I05Rik
BB800286
2.28

1450910_at
Cap2
AV261931
2.28

1434697_at
1110001P04Rik
BI692575
2.28

1452800_a_at
0610008C08Rik
AK002320
2.28

1460672_at
2410002F23Rik
BC016099
2.28

1452812_at
Lphn1
AA987131
2.28

1428941_at
Zmym2
AK017929
2.28

1435706_at
Btbd9
AV232817
2.28

1451348_at
Depdc6
BC004774
2.28

1457267_at
A130010J15Rik
BB667685
2.28

1449048_s_at
Rab4a
NM_009003
2.28

1416583_at
Bad
NM_007522
2.28

1417155_at
Mycn
BC005453
2.28

1443672_at
Lars2
AI415729
2.28

1423206_s_at
2310003F16Rik
W45949
2.27

1456766_at
C330001K17Rik
BB036966
2.27

1448711_at
Mcm3ap
NM_019434
2.27

1459897_a_at
Sbsn
AI507307
2.27

1428626_at
Lysmd2
AK008800
2.27

1424645_at
Tnrc6c
BB795102
2.27

1424997_at
Sfrs8
BC025219
2.27

1439497_at
4933415E08Rik
BG065013
2.27

1422647_at
Ring1
NM_009066
2.27

1460688_s_at
AA407659
BB390422
2.27

1447632_at
Fancc
BB555719
2.27

1418041_at
Tmem186
AW488762
2.27

1437742_at
Rab21
BB767504
2.27

1440285_at
Ppp1r9a
BF472950
2.27

1429874_at
Spata17
AV265007
2.27

1454931_at
Eid2
BE225694
2.27

1438232_at
Foxp2
AV322952
2.27

1429097_at
C030044C12Rik
BB090042
2.27

1433632_at
Irf2bp2
BB183385
2.27

1452521_a_at
Plaur
X62701
2.27

1417410_s_at
Prkci
NM_008857
2.27

1455046_a_at
Pogz
BI250645
2.27

1460739_at
Zmiz2
BQ174371
2.27

1432472_a_at
Mccc2
AK019760
2.27

1419316_s_at
Gnb11
NM_023120
2.27

1434158_at
Gmds
AI747296
2.27

1415941_s_at
Zfand2a
NM_133349
2.27

1434214_at
0910001L09Rik
BI525016
2.27

1426678_at
Zfp706
AK004076
2.27

1419789_at
Centg1
AI605509
2.27

1431385_a_at
Mbtps1
AK002809
2.27

1460679_at
Exosc4
AK005101
2.26

1448875_at
Zhx1
NM_009572
2.26

1423211_at
Nola3
AK004120
2.26

1436335_at
Plch2
BI732150
2.26

1415711_at
Arfgef1
BC025221
2.26

1418716_at
Mrps25
AK004037
2.26

1434980_at
Pik3r5
AV230647
2.26

1431561_a_at
Dhx34
AK007461
2.26

1450250_at
Otud7a
NM_130880
2.26

1433699_at
Tnfaip3
BM241351
2.26

1425051_at
Isoc1
AK010892
2.26

1434254_at
Gna11
BM235739
2.26

1423239_at
Impdh1
BB351792
2.26

1433591_at
Ppp3r1
BF148071
2.26

1434459_at
BC057627
BF147898
2.26

1426430_at
Jag2
AV264681
2.26

1451735_at
Arfrp1
BG695431
2.26

1420825_at
Letm1
BG060855
2.26

1434456_at
Gm440
BG075955
2.26

1447899_x_at
Tacstd1
AV099587
2.26

1428076_s_at
Ndufb4
BG968046
2.26

1460009_at

BB667115
2.26

1451016_at
Ifrd2
BB540964
2.26

1433572_a_at
BC010304
AA673192
2.26

1448915_at
Zfp524
NM_025324
2.26

1420978_at
Nrf1
NM_010938
2.26

1421903_at
Ixl
AK005112
2.26

1425462_at
Fbxw11
AY038079
2.26

1426710_at
Calm3
BB396904
2.26

1449494_at
Rab3c
AY026947
2.26

1449577_x_at
Tpm2
AK003186
2.26

1448720_at
Lrrc40
NM_024194
2.26

1429867_at
4933424C08Rik
AK016884
2.26

1423107_at
Ube2b
AK010432
2.25

1429634_at
Zfp580
AK005257
2.25

1441773_at
1700061N14Rik
AV207074
2.25

1417154_at
Slc25a14
NM_011398
2.25

1450466_at
B230310J22Rik
BI739719
2.25

1458421_at
Kcnq3
AW494964
2.25

1417096_at
Rrp15
NM_026041
2.25

1437389_x_at

AV117555
2.25

1424610_at
Trub2
BG064045
2.25

1423335_at
1110004F10Rik
AW545354
2.25

1425228_a_at
Dguok
AY037862
2.25

1417709_at
Cyp46a1
NM_010010
2.25

1447757_x_at
1110007A13Rik
AV033355
2.25

1426760_at
Ipo8
AW413962
2.25

1428285_at
8430427H17Rik
AK018446
2.25

1424418_at
Slc25a38
BC010801
2.25

1440874_at
Slco5a1
BE686667
2.25

1416714_at
Irf8
BG069095
2.25

1433902_at
Kbtbd8
BB469300
2.25

1429457_at
2310020A21Rik
BB001825
2.25

1456585_x_at
E130309D02Rik
BB546892
2.25

1454680_at
D5Ertd579e
BG073020
2.25

1422767_at
Bys1
BG079188
2.25

1457276_at
Snf1lk2
AI315692
2.25

1439934_at
Slc30a10
BB540543
2.25

1448358_s_at
Snrpg
NM_026506
2.25

1453021_at
Stxbp5
BM899291
2.25

1423231_at
Nrgn
AK002933
2.25

1424006_at
Aarsd1
BC005711
2.25

1428512_at
Bhlhb9
AK012577
2.25

1428266_at
Myl3
AK002312
2.25

1426624_a_at
Ypel3
BI660196
2.25

1416228_at
Pin1
NM_023371
2.24

1434782_at
Usp42
AW551594
2.24

1437994_x_at
Mier2
BB531179
2.24

1428825_at
Nr6a1
AK007201
2.24

1451226_at
Pex6
BC003424
2.24

1422579_at
Hspe1
NM_008303
2.24

1433621_at
Wdr41
BB334979
2.24

1452353_at
Gpr155
BB762731
2.24

1417527_at
Ap3m2
BC027301
2.24

1429039_s_at
1500034J01Rik
BI904336
2.24

1449694_s_at
Commd5
C77953
2.24

1427033_at
Dnmbp
BC025944
2.24

1425264_s_at
Mbp
BB181247
2.24

1418698_a_at
Fech
NM_007998
2.24

1428826_at
Nr6a1
AK007201
2.24

1452054_at
Ube2w
BB796558
2.24

1440716_at
6430604M11Rik
BB188841
2.24

1424629_at
Brca1
U31625
2.24

1435991_at
Nr3c2
BQ174990
2.24

1423262_a_at
H3f3a
BI111967
2.24

1434234_at
Zfp341
BM230261
2.24

1434144_s_at
2410187C16Rik
BG073505
2.24

1418543_s_at
Ccdc43
NM_025918
2.24

1451572_a_at
5230400G24Rik
BC016597
2.24

1447739_x_at
Klhdc4
AV294746
2.24

1447892_at

AV208528
2.24

1453294_at
1700012B15Rik
AK007702
2.24

1416885_at
1110038F14Rik
NM_054099
2.24

1424419_at
Lrrc14
BC022126
2.23

1418899_at
Ufm1
NM_026435
2.23

1425624_at
Epm2aip1
BC018474
2.23

1429901_at
Tcba1
AK018248
2.23

1434116_at
Cbx2
BI693188
2.23

1441295_at
Lman2l
BB712151
2.23

1423945_a_at
Pkig
BC026550
2.23

1428108_x_at
Tmcc2
AK004359
2.23

1457696_at
Rilp
AI835553
2.23

1436265_at
6330405H19
BB225226
2.23

1419353_at
Dpm1
NM_010072
2.23

1424211_at
Slc25a33
BC011293
2.23

1433425_at
1700101I19Rik
AK007108
2.23

1434509_at
Rapgef6
BM250883
2.23

1422589_at
Rab3a
NM_009001
2.23

1431050_at
Rps6ka5
BE291900
2.23

1450204_a_at
Mynn
AK019238
2.23

1455031_at
Cdc2l6
AV278072
2.23

1418545_at
Wasf1
NM_031877
2.23

1439113_at
LOC77114
BB379753
2.23

1419264_at
1200014M14Rik
AK012926
2.23

1460391_at
Gtpbp9
AK013055
2.22

1419717_at
Sema3e
NM_011348
2.22

1424473_at
Polr2h
BC002306
2.22

1444450_at
9530096D07Rik
BB124025
2.22

1415721_a_at
1200013P24Rik
AK004750
2.22

1428177_at
Trappc6b
BG066452
2.22

1459813_at
1700012D01Rik
AV040390
2.22

1417557_at
Ubxd1
NM_024432
2.22

1448598_at
Mmp17
NM_011846
2.22

1458511_at
2410025L10Rik
BE687898
2.22

1452069_a_at
Thap7
BM210680
2.22

1434139_at
Parp11
BB026163
2.22

1455504_a_at
Mkrn1
BE133749
2.22

1460733_at
AA407659
BB390422
2.22

1429588_at
2810474O19Rik
AA509870
2.22

1456480_at
Fry
BB527078
2.22

1422969_s_at
Ihpk1
NM_013785
2.22

1424490_at
Zfp428
BC024802
2.22

1418717_at
Mrps25
AK004037
2.22

1420196_s_at
Tbc1d14
C77429
2.22

1456161_at
0610040B10Rik
BE198048
2.22

1429140_at
9830002I17Rik
AK020683
2.21

1417517_at
Plagl2
NM_018807
2.21

1455286_at
Btbd1
AV018484
2.21

1423259_at
Id4
BB121406
2.21

1434176_x_at
Poldip3
BI963573
2.21

1415936_at
Bcar3
NM_013867
2.21

1452817_at
Smyd3
AK010447
2.21

1426248_at
Stk24
BG060677
2.21

1423378_at
Adam23
AI838132
2.21

1460333_at
Ddx59
BB829482
2.21

1451078_at
2510039O18Rik
BC012878
2.21

1439014_at
1600021P15Rik
BB703667
2.21

1428367_at
Ndst1
BI652065
2.21

1421297_a_at
Cacna1c
NM_009781
2.21

1455547_at
Zc3h7b
BM125518
2.21

1424913_at
2310044G17Rik
AK009800
2.21

1451543_at
Fbxo21
BC021871
2.21

1434253_s_at
Tmcc3
BB711990
2.21

1418327_at
1110058L19Rik
NM_026503
2.21

1443773_at
Ylpm1
AI851834
2.21

1424300_at
Gemin6
BC025157
2.20

1422470_at
Bnip3
NM_009760
2.20

1440932_at

BB428585
2.20

1425628_a_at
Gtf2i
AF043220
2.20

1425333_at
Rab43
BC010248
2.20

1451166_a_at
Ccdc101
BC026784
2.20

1433936_at
0610010E21Rik
BG791555
2.20

1455941_s_at
Map2k5
BB025010
2.20

1416790_a_at
Tdg
NM_011561
2.20

1423649_at
Tmem68
BC016240
2.20

1453566_at
Prr8
AK014289
2.20

1416633_a_at
5730536A07Rik
NM_026635
2.20

1423970_at
Thoc3
BE335845
2.20

1436976_a_at
Pfkfb2
BB201861
2.20

1417387_at
Med31
NM_026068
2.20

1428841_at
Best1
AK006549
2.20

1455723_at
D1Ertd448e
AV279416
2.20

1420012_at
Xbp1
C77390
2.20

1455273_at

BG064695
2.20

1441173_at
4930451G09Rik
AV352442
2.20

1436949_a_at
LOC668554
AV068352
2.20

1430081_at
Phf15
AK004823
2.20

1433878_at
Mrps26
AI648866
2.20

1444026_at
AI593442
BF658761
2.20

1451624_a_at
Phospho2
BC025612
2.20

1418737_at
Nudt2
NM_025539
2.20

1450036_at
Sgk3
BB768208
2.20

1434315_at
Npal3
BB667103
2.20

1435970_at
Nlk
AU035920
2.20

1458755_at

BB125469
2.20

1428468_at
3110043O21Rik
AK014175
2.20

1455798_at
Galk2
BB771515
2.20

1430021_a_at
Sae1
AK011772
2.20

1416910_at
Dnajc15
NM_025384
2.20

1418929_at
Ift57
NM_028680
2.19

1451075_s_at
Ctdsp2
BB294133
2.19

1450520_at
Cacng3
NM_019430
2.19

1452839_at
Dph5
AK010475
2.19

1449733_s_at
Siah1a
AA982064
2.19

1416505_at
Nr4a1
NM_010444
2.19

1433906_at
Asph
BE956483
2.19

1428693_at
2610044O15Rik
AK011776
2.19

1429367_at
Wipi2
BB826276
2.19

1423188_a_at
6720456B07Rik
BI456243
2.19

1415729_at
Pdpk1
BQ174223
2.19

1417916_a_at
Fxc1
NM_019502
2.19

1415746_at
Cic
AF363690
2.19

1425679_a_at
Mapk8ip1
AF109769
2.19

1434196_at
Dnaja4
BQ176119
2.19

1416379_at
Panx1
NM_019482
2.19

1457699_at
E330009J07Rik
BB834854
2.19

1428023_at
3110009E18Rik
AA879840
2.19

1452777_a_at
Nub1
BG066220
2.19

1451188_at
Wdr26
BC020044
2.19

1420460_a_at
Pex11b
NM_011069
2.19

1455588_at
Lyrm4
BE952216
2.19

1457990_at
Anks1b
BB080832
2.19

1459881_at
AI595406
AI595406
2.19

1419122_at
Mettl1
NM_010792
2.19

1434076_at
Wdr37
AV222037
2.19

1424181_at
5-Sep
BC010489
2.19

1434427_a_at
Rnf157
BB246182
2.19

1460553_at
1700025K23Rik
BF466605
2.19

1451568_at
A630054L15Rik
BC025603
2.18

1428071_at
1110038D17Rik
AK004153
2.18

1429042_at
2010200O16Rik
AK008415
2.18

1429378_x_at
AY761184
AK008498
2.18

1418687_at
Arc
NM_018790
2.18

1442120_at

AA153229
2.18

1439393_x_at
Ppp2r4
BB369168
2.18

1448284_a_at
Ndufc1
NM_025523
2.18

1450042_at
Arx
BB322201
2.18

1455234_at
B3galt1
BB795733
2.18

1421823_a_at
Ppp2cb
AI323685
2.18

1434155_a_at
2310061I04Rik
BB174350
2.18

1437918_at
4930539E08Rik
AV374644
2.18

1460453_at
Tagap
BM220475
2.18

1428317_at
Fundc2
AI510221
2.18

1434995_s_at
Dedd
BE199280
2.18

1424172_at
Hagh
BC004749
2.18

1452331_s_at
Qser1
BC021511
2.18

1433700_at
4933433P14Rik
BB477807
2.18

1436573_at
Scrn3
BB434447
2.18

1460320_at
Becn1
NM_019584
2.18

1423737_at
Ndufs3
BC027270
2.18

1419357_at
Isy1
NM_133934
2.17

1431804_a_at
Sp3
AK004607
2.17

1451390_s_at
Zfand2b
BC011495
2.17

1449611_at
Cd82
AI894122
2.17

1451005_at
Sumo1
BM209585
2.17

1451574_at
Bcl9
BC019641
2.17

1453406_a_at
Rab28
AK012286
2.17

1426939_at
Tsr2
BG070464
2.17

1424250_a_at
Arhgef3
BC012262
2.17

1447991_at
Pcsk2
AI839700
2.17

1436596_at
H2afv
BG092470
2.17

1437082_at
Akap9
BB246410
2.17

1445194_at
Cnksr2
BB355006
2.17

1417903_at
Dfna5h
NM_018769
2.17

1423663_at
Flcn
BC025820
2.17

1429213_at
2310030N02Rik
AK009547
2.17

1459544_at

BB180990
2.17

1456320_at
BC049806
BB701297
2.17

1428600_at
Nin
AK014241
2.17

1419444_at
Sap18
NM_009119
2.17

1436405_at
Dock4
BG068753
2.17

1455991_at
Ccbl2
BG094881
2.17

1436984_at
Abi2
BG261647
2.17

1449183_at
Comt
NM_007744
2.17

1435087_at
BC039093
BI409907
2.17

1415688_at
Ube2g1
NM_025985
2.16

1434598_at
Larp5
BM200204
2.16

1450024_at
Sufu
AJ308624
2.16

1460188_at
Ptpn6
NM_013545
2.16

1452237_at
Hrb
BB130716
2.16

1446775_at

C76272
2.16

1417067_s_at
Cabc1
AK014605
2.16

1434266_at
AI847670
AI847670
2.16

1415755_a_at
Ube2v1
BC019372
2.16

1422119_at
Rab5b
NM_011229
2.16

1452776_a_at
Nub1
BG066220
2.16

1429073_at
2210015D19Rik
AK005556
2.16

1443733_x_at
Pold3
C85233
2.16

1433544_at
Als2cr2
BB277912
2.16

1456089_at
Trim23
AV276781
2.16

1418048_at
1110059G10Rik
NM_025419
2.16

1424028_at
5830457O10Rik
BC023107
2.16

1422516_a_at
Fibp
NM_021438
2.16

1455003_at
AW490810
BQ032496
2.16

1447994_at
1700026B20Rik
AV043099
2.16

1429389_at
Setmar
AK017895
2.16

1429151_at
Wdr68
AK011714
2.16

1452923_at
Ndufa121
AK007894
2.16

1455042_at
Tbl1x
BF682509
2.16

1456332_at
Tmem17
AV290482
2.16

1455149_at
Sh3rf1
BB072090
2.16

1435827_at
4933404O12Rik
BB501711
2.15

1439253_x_at
2610524G07Rik
AV111335
2.15

1423313_at
Pde7a
BG070255
2.15

1430361_at
2610005M20Rik
BC003771
2.15

1452096_s_at
D230025D16Rik
BG065702
2.15

1440427_at
BB124205
BB127176
2.15

1437617_x_at
1110034G24Rik
BB387677
2.15

1429296_at
Rab10
BF465974
2.15

1450103_a_at
Pscd2
NM_011181
2.15

1455117_at
Mcmdc1
AV274182
2.15

1417833_at
Zc3h10
NM_134003
2.15

1417873_at
Pwp1
BC003199
2.15

1441942_x_at
Snupn
AV331209
2.15

1438009_at
Hist1h2ao
W91024
2.15

1424446_at
Armc7
BC023126
2.15

1447905_x_at
Nup62
BB331755
2.15

1431250_at
9030624G23Rik
BG923655
2.15

1428160_at
Ndufab1
AK010307
2.15

1453747_at
2810021J22Rik
BB620330
2.15

1418726_a_at
Tnnt2
NM_011619
2.15

1427163_at
Ubr2
AI646734
2.15

1458932_at
Pex2
BB639093
2.15

1433639_at
5730593F17Rik
AW548096
2.15

1451305_at
Pgea1
AF331040
2.15

1431230_a_at
Btbd9
Z74630
2.15

1456685_at
Nsg2
BB227199
2.15

1422980_a_at
Bet11
NM_018742
2.15

1451747_a_at
Atg12
AK016474
2.15

1460713_at
BC048355
BE949497
2.15

1452584_at
1500032L24Rik
BG915677
2.15

1428252_at
Chmp2b
AA881383
2.15

1427471_at
Fbxl3
BF782863
2.15

1436186_at
E2f8
BM247465
2.15

1451180_a_at
Nt5c3l
BC015307
2.15

1420540_a_at
Rit1
NM_009069
2.15

1418120_at
Rbm8a
NM_025875
2.15

1424837_at
Rnf113a1
BC024906
2.15

1452888_at
1110034G24Rik
AK004090
2.14

1423857_at
Mrpl30
BC004614
2.14

1416755_at
Dnajb1
AK002290
2.14

1453016_at
2900042B11Rik
AK013636
2.14

1415714_a_at
2610209M04Rik
BC027564
2.14

1434341_x_at
1110020P15Rik
BF681728
2.14

1434250_at
Pak2
AW537308
2.14

1426828_at
1300018I17Rik
AK005046
2.14

1418314_a_at
A2bp1
NM_021477
2.14

1434134_at
Wdr42a
BB123403
2.14

1455139_at
Smarcal1
BB028226
2.14

1432000_a_at
Dedd
AK006814
2.14

1453048_at
Nhlrc2
AK002650
2.14

1459021_at

BB307071
2.14

1422677_at
Dgat2
AK002443
2.14

1451955_a_at
Cacna2d2
AF247140
2.14

1453865_a_at
Otud5
AK010750
2.14

1419056_at
Rtn2
AF038538
2.14

1433628_at
Coq10a
BQ044689
2.14

1452601_a_at
Acbd6
AK002470
2.14

1434042_s_at
Mtmr3
BF466640
2.14

1452938_at
Anks1b
BQ174247
2.14

1457256_x_at
Ptch2
BB530125
2.14

1434104_at
Slc35e1
BB041864
2.14

1434218_at
C330019G07Rik
BB400635
2.14

1452222_at
Utrn
AI788797
2.14

1449290_at
Dpysl5
NM_023047
2.14

1448853_at
Synj2bp
NM_025292
2.14

1430068_at
C030011L09Rik
BB356045
2.14

1452207_at
Cited2
Y15163
2.14

1437907_a_at
Tbca
BB559082
2.14

1425710_a_at
Homer1
AB019479
2.14

1456218_at
Snx22
AV333851
2.14

1419964_s_at
Hdgf
C80147
2.14

1420008_s_at
Wwc1
AU017197
2.14

1428971_at
Ccny
AK014507
2.14

1425460_at
Mtmr2
BB197262
2.14

1426998_at
Zfand3
BG976649
2.14

1420852_a_at
B3gnt2
AV306734
2.14

1436309_at
Neto2
BB125651
2.13

1422859_a_at
Rpl23
NM_022891
2.13

1418355_at
Nucb2
NM_016773
2.13

1424922_a_at
Brd4
BC008532
2.13

1449600_at
Gm1683
AA682050
2.13

1427341_at
E130103I17Rik
U85993
2.13

1423087_a_at
1110002E23Rik
BB453951
2.13

1428823_at
Hddc2
AK009957
2.13

1440655_at

BB076281
2.13

1451265_at
Ccdc115
BC019430
2.13

1426496_at
Wdr55
AK017695
2.13

1417481_at
Ramp1
NM_016894
2.13

1419753_at
Nfx1
AK013866
2.13

1424545_at
BC003965
W59564
2.13

1457456_at
Ttc9b
AI481735
2.13

1423240_at
Src
BG868120
2.13

1423906_at
Hsbp1
AK010939
2.13

1459271_at

BB036541
2.13

1448196_at
Mat2b
NM_134017
2.13

1441083_at
Clptm1
BB799287
2.13

1439267_x_at
Cox5a
AV085180
2.13

1437152_at
Rkhd3
BG072837
2.13

1418256_at
Srf
BI662291
2.13

1433855_at
Abat
BF462185
2.13

1443405_at

BB201852
2.13

1454986_at
Zfp668
BB152304
2.13

1429869_at
1110020C03Rik
AI561792
2.13

1431198_x_at
9430038I01Rik
AK020460
2.13

1429380_at
Rgs12
AK004813
2.13

1455517_at
Rbm4
BB419586
2.13

1425181_at
Sgip1
AV344708
2.13

1451570_a_at
6720467C03Rik
BC020162
2.13

1450675_at
Smap11
NM_133716
2.13

1435197_at
Pou3f3
BE993443
2.13

1428989_at
0710001D07Rik
AK002941
2.12

1451385_at
2310056P07Rik
BC010826
2.12

1427874_at
Zfp313
AF502145
2.12

1417105_at
Trappc2l
NM_021502
2.12

1434092_at
Atg9b
AW121498
2.12

1434339_at
Fnbp11
AW548221
2.12

1433711_s_at
Sesn1
BG076140
2.12

1436620_at
Ccdc45
BQ173899
2.12

1430832_at
4930426I24Rik
BB055339
2.12

1422155_at
Hist2h3c2
BC015270
2.12

1419462_s_at
Gtl3
NM_008187
2.12

1434951_at
Armc8
BE995635
2.12

1451943_a_at
Ppm1a
AF259673
2.12

1438039_at
Hectd1
BE630599
2.12

1416775_at
2310004L02Rik
NM_025504
2.12

1417848_at
Zfp704
AW413620
2.12

1436449_at

BB284627
2.12

1446793_at

BB091198
2.12

1449880_s_at
Bglap1
NM_007541
2.12

1432431_s_at
2900006F19Rik
AK004072
2.12

1438692_at
Gtf3c4
BG069995
2.12

1418593_at
Taf6
NM_009315
2.12

1440224_at
9330133O14Rik
BB077224
2.12

1452140_at
Tbc1d20
BC002196
2.12

1434305_at
U2af1l4
BF018149
2.12

1415717_at
4931406I20Rik
BI735988
2.12

1458350_at

AV290089
2.12

1424075_at
9430016H08Rik
BC024945
2.12

1459783_s_at
Cno
AV218165
2.12

1424057_at
Gdap2
BC025070
2.12

1454848_at
Ppp1r12c
BE628628
2.12

1442303_at

AV139114
2.12

1425531_at
Znhit1
BC026751
2.12

1428757_at
Aasdhppt
AK013111
2.12

1423635_at
Bmp2
AV239587
2.12

1448240_at
Mbtps1
NM_019709
2.12

1451290_at
Map1lc3a
BC010596
2.12

1450926_at
Atp6v1g1
BI154058
2.12

1436004_at
Usp27x
BB021271
2.11

1443836_x_at
Wdr48
BB064885
2.11

1456420_at
Arid4a
BB667227
2.11

1427565_a_at
Abcc5
AF213387
2.11

1434934_at
Atpaf1
BB771055
2.11

1423675_at
Usp1
BC018179
2.11

1434483_at
Usp12
AW539189
2.11

1427131_s_at
Actb
AV234245
2.11

1441764_at
Prdm10
BF730739
2.11

1451124_at
Sod1
BC002066
2.11

1428405_at
Hcfc1r1
BF580567
2.11

1425601_a_at
Rtkn
BC013820
2.11

1454722_at
Pten
BG792618
2.11

1424313_a_at
Ndufs7
BC013503
2.11

1421315_s_at
Cttn
BI688355
2.11

1429119_at
Iah1
AK005287
2.11

1437350_at
RP23-143A14.5
AV290046
2.11

1456109_a_at
Mrps15
BB314055
2.11

1451303_at
BC002230
BC025577
2.11

1423736_a_at
Dym
BC018220
2.11

1423245_at
Cops7a
BG974128
2.11

1428717_at
Scrn1
AW490544
2.11

1424529_s_at
Cgref1
BC023116
2.11

1433498_at
2010005J08Rik
BF585125
2.11

1416668_at
Ttc35
NM_025736
2.11

1418632_at
Ube2h
BI694835
2.11

1428898_at
Mon1a
AK013387
2.11

1430423_s_at
2610528E23Rik
AK019808
2.11

1441203_at

BB097040
2.11

1438170_x_at
Adrm1
AV166873
2.11

1416920_at
Rbm4
NM_009032
2.11

1448963_at
Nfyc
BC020117
2.11

1419379_x_at
Fxyd2
NM_052823
2.11

1455659_at
Tmcc1
AA244468
2.11

1443987_at
Klhl18
BB283043
2.11

1428106_at
1300001I01Rik
AK004841
2.11

1432287_a_at
Sntg1
AK016927
2.11

1435566_s_at
Araf
BG228947
2.10

1433539_at
Commd3
BB230296
2.10

1448685_at
2900010M23Rik
NM_026063
2.10

1434071_a_at
Itga1
AV288264
2.10

1438857_x_at

BB058253
2.10

1419803_s_at
Ccdc12
C76605
2.10

1440534_at

BB177862
2.10

1442994_at
Sod2
BQ177195
2.10

1433464_at
Ipo13
BB475675
2.10

1451126_at
Maf1
BC016260
2.10

1452379_at
Auts2
AK012475
2.10

1431087_at
Spbc24
BF577722
2.10

1434083_a_at
Elmod1
BI738305
2.10

1436990_s_at
Ndg2
AA038464
2.10

1448533_at
Tbcb
NM_025548
2.10

1453364_x_at
9130019O22Rik
AK020267
2.10

1438769_a_at
Vps26b
BF719766
2.10

1424199_at
Seh11
AW537349
2.10

1451408_at
Trub2
BG064045
2.10

1434435_s_at
Cox17
BM941165
2.10

1434019_at
Pdap1
BG065186
2.10

1420640_at
Jmy
BF227962
2.10

1457342_at
Ikzf4
BM114149
2.10

1416477_at
Ube2d2
NM_019912
2.10

1416550_at
Slc35b4
BB320416
2.10

1421051_s_at
Vps25
NM_026776
2.10

1424399_at
Uck1
BC025146
2.10

1426729_at
2900046G09Rik
BC003957
2.10

1420367_at
Denr
AK010394
2.10

1455959_s_at
Gclc
AW825835
2.10

1450890_a_at
Abi1
AW912678
2.10

1431463_at
1700041C02Rik
AK015391
2.10

1443932_at
Klhdc1
BQ176961
2.09

1435591_at
AI426330
AW495162
2.09

1417609_at
Ube2a
BG868960
2.09

1423053_at
Arf4
BI653265
2.09

1434367_s_at
Nutf2
AV217767
2.09

1415799_at
Wbp11
NM_021714
2.09

1424013_at
Etf1
BC013717
2.09

1449406_at
Cyhr1
NM_019396
2.09

1456538_at
Sdccag8
BM233019
2.09

1426219_at
Scp2
M62361
2.09

1435705_at
LOC232875
BB305660
2.09

1423990_at
Rab28
BC004580
2.09

1420846_at
Mrps2
AV031454
2.09

1418955_at
Zfp93
NM_009567
2.09

1451811_at
Cacng6
AV091458
2.09

1424784_at
6330416L07Rik
AV047635
2.09

1448957_at
Rbpsuh
NM_009035
2.09

1449099_at
Lrba
NM_030695
2.09

1426401_at
Ppp3ca
BQ173889
2.09

1455020_at
Snx25
AV351081
2.09

1434438_at
Samhd1
BF148012
2.09

1440947_at
Bat2d
BB481861
2.09

1452690_at
Khsrp
BQ174458
2.09

1427003_at
Ppp2r5c
BG072817
2.09

1442999_at
B930036G03Rik
BB342280
2.09

1453718_at
Bcl2l12
AK013411
2.09

1448403_at
Lars
NM_134137
2.09

1454866_s_at
Clic6
BQ176424
2.09

1446624_at
Fcmd
BB518629
2.09

1423632_at
Gpr146
BI103049
2.09

1452721_a_at
Ccdc53
BM210204
2.09

1429958_x_at
Haghl
BI790001
2.09

1438196_at
Gpd11
BB727537
2.09

1421524_at
Cfc1
NM_007685
2.09

1460363_at
Tnrc6c
BB795102
2.09

1436522_at
Map3k3
BE948629
2.09

1428578_s_at
Ppfia4
AK003571
2.08

1455187_at
Zbtb40
BM231844
2.08

1460691_at
Zfp598
BF385956
2.08

1455507_s_at
D8Ertd587e
BM223036
2.08

1418996_a_at
Lyrm5
BC021522
2.08

1449287_at
Srms
NM_011481
2.08

1429683_at
5830472M02Rik
BG094398
2.08

1426493_a_at
Kifc2
BB798224
2.08

1436347_a_at
5530601H04Rik
BB501229
2.08

1417211_a_at
1110032A03Rik
NM_023483
2.08

1422844_a_at
Wdr77
NM_027432
2.08

1452174_at
Srebf2
BM123132
2.08

1418568_x_at
Srp14
NM_009273
2.08

1419026_at
Daxx
NM_007829
2.08

1434488_at
Arfrp1
BQ176611
2.08

1419912_s_at
Strap
AW557906
2.08

1438360_x_at
Slc25a5
AV003169
2.08

1441578_at
Ccdc86
BB142123
2.08

1417825_at
Esd
NM_016903
2.08

1431059_x_at
Htatsf1
AK012522
2.08

1443401_at
Astn2
BM941510
2.08

1426643_at
Elp3
AK012072
2.08

1455073_at
Cdadc1
BB178837
2.08

1420483_at
Cnnm3
NM_053186
2.08

1448138_at
Ppp2r4
NM_138748
2.08

1428806_at
Csnk1g1
AK018639
2.08

1435153_at
Btbd6
AV026558
2.08

1431170_at
Efna3
AA388313
2.08

1431354_a_at
Fars2
AK020164
2.08

1453106_a_at
Rnmt
AK015403
2.08

1424982_a_at
2700078E11Rik
BB137173
2.08

1417591_at
Ptges2
NM_133783
2.08

1420611_at
Prkacb
AV024339
2.08

1417320_at
Grpel1
NM_024478
2.08

1451412_a_at
Ift20
AY082613
2.08

1438324_at
9330182L06Rik
AW550882
2.08

1455583_at
Gne
AV371374
2.08

1419805_s_at
Ggps1
C79210
2.07

1426980_s_at
E130012A19Rik
BC006054
2.07

1451143_at
1110006G06Rik
BC023421
2.07

1416506_at
Psma6
NM_011968
2.07

1421066_at
Jak2
NM_008413
2.07

1434968_a_at
Actr3
BB758476
2.07

1460342_s_at
AA536749
U73200
2.07

1457139_at
Auts2
AV021813
2.07

1449162_at
Pop7
NM_028753
2.07

1423777_at
Usp20
AK006800
2.07

1454855_at
Magi2
BQ176520
2.07

1428760_at
Snapc3
AW537061
2.07

1416776_at
Crym
NM_016669
2.07

1420888_at
Bcl2l1
NM_009743
2.07

1436008_at
Tpd52
AV336744
2.07

1433505_a_at
Lrrc8d
BB315861
2.07

1419454_x_at
Pias2
NM_008602
2.07

1423962_at
Wdr26
BC020044
2.07

1416834_x_at
Ndufb2
NM_026612
2.07

1426017_a_at
0610011L14Rik
BC020112
2.07

1426355_a_at
6330578E17Rik
BB667858
2.07

1421662_a_at
Tusc3
NM_030254
2.07

1421399_at
Insm1
NM_016889
2.07

1423303_at
Paxip1
AW742928
2.07

1421144_at
Rpgrip1
NM_023879
2.07

1437456_x_at
Ythdf1
BB332410
2.07

1453342_at
Cdc40
AK004569
2.07

1425313_at
Carf
AF454947
2.07

1421600_a_at
Trim26
NM_030698
2.07

1429610_a_at
Zfp511
AK010407
2.06

1419630_a_at
Trim11
NM_053168
2.06

1431091_at
Pygo1
BB595944
2.06

1434994_at
Dedd
BE199280
2.06

1452914_at
2410024N18Rik
AK010587
2.06

1445763_at
1700013F07Rik
BG069737
2.06

1428745_a_at
2310003L22Rik
AK009123
2.06

1450894_a_at
Ap2m1
C76671
2.06

1428681_at
5530400K22Rik
AK017426
2.06

1423668_at
Zdhhc14
BC021423
2.06

1435092_at
Arl4a
AV328143
2.06

1457171_at
AI854517
BB334885
2.06

1443528_at
Ttbk1
BB398560
2.06

1424291_at
Nup93
BC023140
2.06

1436034_at
Cep68
AW550283
2.06

1429985_at
5430439G13Rik
BQ173897
2.06

1452171_at
Grwd1
BM200151
2.06

1458541_at
Dctn4
BB125218
2.06

1426467_s_at
0610037L13Rik
AK002776
2.06

1430769_s_at
2900009I07Rik
AW911794
2.06

1452050_at
Camk1d
BG071931
2.06

1416918_at
Dlg3
NM_016747
2.06

1420660_at
Lrrc6
NM_019457
2.06

1418975_at
Nckipsd
NM_030729
2.06

1453795_at
Fahd2a
BI872590
2.06

1426427_at
Ttll1
BB764137
2.06

1443790_x_at
4930414L22Rik
AV208084
2.06

1453709_at
3426406O18Rik
AV172660
2.06

1418665_at
Impa2
NM_053261
2.06

1433707_at
Gabra4
BB430205
2.06

1425837_a_at
Ccrn4l
AF199491
2.06

1435500_at
Rab26
BB270778
2.06

1451301_at
Tmod2
BB633110
2.06

1456769_at
Dusp3
BQ266434
2.06

1443826_x_at
Men1
BB269348
2.06

1423460_at
Perq1
BI688428
2.06

1439900_at
Thtpa
BB308405
2.06

1452056_s_at
Ppp3ca
BQ173889
2.06

1456423_at
Ap2m1
AV298746
2.06

1449614_s_at
AI314976
AW558560
2.05

1437293_x_at
Ust
BB561487
2.05

1431985_at
2210010B09Rik
AK008693
2.05

1459760_at
Ndufs4
BE852624
2.05

1419070_at
Cys1
BF466918
2.05

1421945_a_at
Bxdc1
NM_023323
2.05

1415678_at
Ppm1a
BC008595
2.05

1426909_at
Uck2
BC004016
2.05

1428345_at
Ppapdc2
BB229589
2.05

1448455_at
Cln8
AF125307
2.05

1436354_at
Dzip11
BE992423
2.05

1460635_at
Fastk
NM_023229
2.05

1458247_s_at
Dctn5
C78178
2.05

1434383_at
Pja2
BM114949
2.05

1425410_at
Tprkb
BC027413
2.05

1442003_at

BF537922
2.05

1450706_a_at
Arl3
NM_019718
2.05

1436892_at
Spred2
BB133520
2.05

1431885_a_at
Mus81
AK004647
2.05

1424054_at
Btbd2
BC016566
2.05

1420443_at
Pcdhb19
NM_053144
2.05

1447926_at
Arl5a
BB811124
2.05

1434451_at

AA216953
2.05

1423440_at
1110001A07Rik
AK003196
2.05

1423453_at
Nol12
BI408798
2.05

1429383_at
Csnk1g3
BM195380
2.05

1455421_x_at
6330503C03Rik
AW490145
2.05

1438649_x_at
Pebp1
AV207950
2.05

1422885_at
Snrpd3
NM_026095
2.05

1430588_at
Mro
BI076815
2.05

1440459_at
Setx
BB767941
2.05

1415998_at
Vdac1
NM_011694
2.05

1416867_at
Bet1
NM_009748
2.05

1417126_a_at
Rpl22l1
NM_026517
2.04

1451108_at
Rnf185
BC014812
2.04

1434652_at
Cdc42bpb
BI154551
2.04

1440238_at
Gltscr1
BB158599
2.04

1429411_a_at
Eny2
AI595744
2.04

1422564_at
Actl6b
NM_031404
2.04

1441284_at

BB283819
2.04

1430017_at
1600016N20Rik
AK005476
2.04

1426649_at
Tmeff1
BM114154
2.04

1417169_at
Usp2
AI553394
2.04

1456602_at
4932417I16Rik
AV274211
2.04

1455939_x_at
Srp14
AV209126
2.04

1457826_a_at
1810043H04Rik
AV056885
2.04

1418646_at
Gna-rs1
BF577955
2.04

1434879_at
Cdc34
BI794243
2.04

1453132_a_at
Gkn2
AK007451
2.04

1416208_at
Usp14
BC005571
2.04

1438847_at
Mxd3
BB836571
2.04

1416852_a_at
Ncdn
BC017126
2.04

1434891_at
Ptgfrn
AV253087
2.04

1418302_at
Ppt2
BC013462
2.04

1460381_at
BC023179
BC023179
2.04

1429156_at
2610036L11Rik
BF453953
2.04

1423976_at
4930453N24Rik
BC020029
2.04

1420734_at
Ppp1r3f
NM_138605
2.04

1422849_a_at
Pabpn1
AV028400
2.04

1432003_a_at
Rnf41
AK016825
2.04

1422091_at
Pfkfb2
BC018418
2.04

1434095_at
Unc5a
BM226712
2.04

1455118_at
D9Ertd402e
BM122201
2.04

1419415_a_at
Rarg
NM_011244
2.04

1449018_at
Pfn1
NM_011072
2.04

1436813_x_at
Khsrp
BB332580
2.04

1449851_at
Per1
AF022992
2.04

1427908_at
Bnip1
BG073508
2.04

1432435_s_at
C030004A17Rik
AK012261
2.04

1427025_at
Mtmr7
AV094609
2.04

1426256_at
Timm17a
AF106620
2.04

1424170_at
Phf5a
BC025161
2.04

1426924_at
Rc3h2
AA709668
2.03

1448356_at
Ube2d2
NM_019912
2.03

1417847_at
Ulk2
NM_013881
2.03

1451442_at
Ccdc104
BC005731
2.03

1418966_a_at
Dcbld1
AK014521
2.03

1428246_at
Vps26b
BI081895
2.03

1423757_x_at
Igfbp4
BC019836
2.03

1429050_at
Chic2
AK015681
2.03

1450386_at
Kpna3
BM213828
2.03

1451343_at
Vps36
BC010811
2.03

1444208_at
Dnahc1
BE950899
2.03

1428075_at
Ndufb4
BG968046
2.03

1452635_x_at
Josd3
AU044383
2.03

1437937_at
Ccbp2
AV220666
2.03

1438412_at
Phf17
BM119726
2.03

1441376_at
Gabarapl2
BB426412
2.03

1423370_a_at
Csnk1g2
AI326320
2.03

1449702_at
Zfand2a
AU016206
2.03

1457272_at

BB284000
2.03

1441782_at
Aak1
AW121504
2.03

1442046_at
Shroom2
BM119167
2.03

1451736_a_at
Map2k7
AW541674
2.03

1460452_at
Mospd3
BC003880
2.03

1422018_at
Hivep2
NM_010437
2.03

1452626_a_at
1810014F10Rik
AK007494
2.03

1435072_at
Zfyve1
AV327165
2.03

1416748_a_at
Mre11a
NM_018736
2.03

1418117_at
Ndufs4
NM_010887
2.03

1433897_at
AI597468
BQ176744
2.03

1447689_at
Gprasp1
AV221773
2.03

1451664_x_at
Klra7
AF283248
2.03

1460209_at
Usp39
BC026983
2.03

1455841_s_at
Grwd1
BB251524
2.03

1435645_at
Mmd
AA472735
2.03

1453569_s_at
Mark4
AK010751
2.03

1427426_at
Kcnq5
AV362204
2.03

1426692_at
Ccdc97
AK004762
2.02

1452324_at
Pvt1
BE956863
2.02

1453002_at
Sox11
BE825056
2.02

1421520_at
Jph1
NM_020604
2.02

1430500_s_at
Mtx2
AK005233
2.02

1449711_at
Atp6v1e1
C85064
2.02

1420969_at
Btbd14b
NM_025788
2.02

1428756_at
Aasdhppt
AK013111
2.02

1418384_at
Apool
BC024334
2.02

1460697_s_at
2610209M04Rik
BC027564
2.02

1434165_at
Clic6
BQ176424
2.02

1433970_at
Bola3
BB610351
2.02

1416240_at
Psmb7
NM_011187
2.02

1450908_at
Eif4e
BB406487
2.02

1416192_at
Napa
BC004804
2.02

1435105_at
1110061N23Rik
BG066986
2.02

1456149_at
D11Bwg0517e
BB386621
2.02

1456125_a_at
EG627788
AV006937
2.02

1427702_at
Zfp1
X16493
2.02

1443875_at
Gm603
BG075083
2.02

1428498_at
2610206B13Rik
AK011896
2.02

1454236_a_at
C030004A17Rik
AK012261
2.02

1434009_at
Grlf1
BQ176422
2.02

1448762_at
Rad17
NM_011233
2.02

1457778_at
Stox2
BB364290
2.02

1450080_at
Cxx1c
NM_028375
2.02

1431510_s_at
2010110K16Rik
AK008567
2.02

1448247_at
Bcl7b
NM_009745
2.02

1426763_at
Oaz2
AW214584
2.02

1418905_at
Nubp1
NM_011955
2.02

1439553_s_at
Nutf2
AU018817
2.02

1437242_at
Ttl112
AV121345
2.02

1452753_at
Foxk2
BM206907
2.02

1423146_at
Hes5
AV337579
2.02

1456032_x_at
H2afz
AV215230
2.02

1435886_at
Dnajb12
AW456220
2.02

1456013_x_at
Slc35a4
BB396486
2.01

1428234_at
Cpsf6
BB425379
2.01

1428689_at
Tysnd1
BF466063
2.01

1454769_at
Tatdn2
BB314680
2.01

1422750_a_at
Zmynd10
NM_053253
2.01

1435432_at
Centg2
BE688580
2.01

1429799_at
Zfp606
BB541617
2.01

1428513_at
Calcoco1
AK007393
2.01

1416698_a_at
Cks1b
NM_016904
2.01

1416373_at
Nfs1
NM_010911
2.01

1460562_at
Eftud1
BB042938
2.01

1445221_at

BB451452
2.01

1430675_at
2900055J20Rik
BM932606
2.01

1419736_a_at
Eiflay
NM_025437
2.01

1423738_at
Oxa11
BC027191
2.01

1459846_x_at
Tmem4
AV100225
2.01

1425621_at
Trim35
AB060155
2.01

1451207_at
Cbara1
BC023022
2.01

1429796_at
Kalrn
AK008844
2.01

1430292_a_at
1810030N24Rik
BG792484
2.01

1426665_at
Katnb1
AK010364
2.01

1455303_at
Rfxap
BB735651
2.01

1426613_a_at
Snrpb2
AV066554
2.01

1422560_at
Rsc1a1
NM_023544
2.01

1437985_a_at
2310061I04Rik
BB379386
2.01

1421891_at
St3gal2
BB750118
2.01

1430569_at
Ttc9c
AK008753
2.01

1427915_s_at
Tceb1
AI019214
2.01

1425780_a_at
Tmem167
BC024352
2.01

1450394_at
Golph3
AV174110
2.01

1416499_a_at
Dctn6
NM_011722
2.01

1434249_s_at
Trim9
BB083438
2.01

1430030_at
5330426P16Rik
AK019912
2.01

1423479_at
Nol11
AK005123
2.01

1459110_at
Myo9b
BB344956
2.01

1416369_at
Hiatl1
NM_133680
2.01

1456444_at
Fbxo41
BB126849
2.01

1419975_at
Scp2
C76618
2.01

1447889_x_at
Gm50
BB172440
2.01

1431505_at
Ppih
AK008394
2.01

1418404_at
Rad9
NM_011237
2.01

1458103_at
Ncor1
AV325208
2.01

1428638_at
Efhc2
BE956904
2.01

1423617_at
Pdf
AA790871
2.01

1419927_s_at
Rabif
AW549708
2.01

1455522_at
Arhgef15
AW492648
2.01

1417930_at
Nab2
NM_008668
2.01

1434207_at
2900057K09Rik
BE570729
2.01

1424121_at
Commd1
AB076722
2.00

1439874_at
9330102E08Rik
BB075161
2.00

1434230_at
Polb
BG094331
2.00

1428213_at
Nsmce4a
AK010349
2.00

1450909_at
Eif4e
BB406487
2.00

1423789_at
BC005624
BC005624
2.00

1428500_at
2210419D22Rik
AK008985
2.00

1420106_at
Siah1a
AA982064
2.00

1434549_at
Rab11a
BI083615
2.00

1424685_at
Exosc4
AK005101
2.00

1415864_at
Bpgm
NM_007563
2.00

1428901_at
Dtwd2
AK004450
2.00

1436438_s_at
Wdr22
BM234499
2.00

1424703_at
Hemk1
BC011431
2.00

1419190_at
Vti1a
BC019386
2.00

1419460_at
Rpp14
BI455861
2.00

1438289_a_at
Sumo1
AV021595
2.00

1417033_at
Ube2g2
AF296657
2.00

1457325_at
Kcns2
BB442995
2.00

1452605_at
Thnsl1
AV254642
2.00

1437132_x_at
Nedd9
BB535494
2.00

1445631_at
Tmem16c
BE865226
2.00

1433774_x_at
Cog1
AA238341
2.00

1428828_at
Rpain
AK010289
2.00

1419066_at
Ier5l
NM_030244
2.00



Fold change values (FCH) are given relative to MSN enrichment.

Probe = Affymetrix probe identification;

Symbol = official gene symbol.


















































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































5) Grouping Enriched Genes According to Biological Function
To group striatonigral- and striatopallidal-enriched genes according to biological function, statistically over-represented Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms associated with these genes were searched. GO terms delineate the known molecular function, biological process, as well as the cellular localization (component) for a particular gene (R. Bernardi, P. P. Pandolfi, Nat Rev Mol Cell Biot 8, 1006 (2007), while KEGG pathways summarize known molecular interaction and reaction networks. The most enriched striatonigral KEGG pathways included neuroactive ligand-receptor interaction, long-term depression, and MAPK signaling pathway. The most striatopallidal enriched KEGG pathways included pyrimidine metabolism, purine metabolism, and neuroactive ligand-receptor interaction. Comparison of striatonigral and striatopallidal cell data versus whole brain revealed many striatal-enriched KEGG pathways, including MAPK signaling pathway, long-term potentiation, and insulin signaling pathway. Striatonigral enriched GO molecular function terms included GTPase activity, calcium ion binding, and retinal binding; striatopallidal enriched GO molecular function terms included adrenoceptor activity, calcium channel activity, and rhodopsin-like receptor activity; striatal-enriched GO terms included zinc ion binding, protein serine/threonine kinase activity, and ubiquitin-protein ligase activity.
Example 11
Physiological Differences Between Striatonigral and Striatopallidal Cells
Differentially translated mRNAs discovered in previous examples immediately predict physiological differences between striatonigral and striatopallidal cells. For example, an mRNA selectively enriched in striatopallidal neurons is Gpr6 (S. Gong et al., Nature 425, 917-25 (Oct. 30, 2003).). Gpr6 encodes a G protein-coupled receptor for the lysophospholipid sphingosine 1-phosphate (S1P) (A. Ignatov, J. Lintzel, H. J. Kreienkamp, H. C. Schaller, Biochem Biophys Res Commun 311, 329-36 (Nov. 14, 2003)). In heterologous expression systems, S1P activation of Gpr6 receptors induces the release of Ca2+ from intracellular stores. As intracellular Ca2+ is a crucial regulator of neuronal physiology, whether selective enrichment of Gpr6 in striatopallidal neurons correlated with a differential response to S1P was investigated. To determine whether functional Gpr6 receptors were selectively expressed in striatopallidal neurons, BAC D2 striatopallidal or BAC D1 striatonigral medium spiny neurons (expressing soluble eGFP) were identified in brain slices and patch clamped ( FIG. 30 ) (M. Day et al., Nat Neurosci 9, 251-9 (February, 2006)). Neurons were loaded with Alexa 594 (50 μM) to allow dendrites to be seen and with Fluo-4 (200 μM) to monitor intracellular Ca2+ concentration using 2-photon laser scanning microscopy (2PLSM). After allowing the dyes to equilibrate, neurons were imaged and a second pipette containing S1P (10 μM) was brought into close physical proximity to a dendrite, 60-80 microns from the soma (a and b). With the somatic membrane potential clamped at −70 mV, focal application of S1P consistently and reversibly increased dendritic Ca2+ levels in BAC D2 striatopallidal neurons (Kruskal-Wallis ANOVA, p<0.01, n=6) but not in BAC D1 striatonigral neurons (Kruskal-Wallis ANOVA, p>0.01, n=4). Depletion of intracellular Ca2+ stores with the Ca2+-ATPase inhibitor thapsigargin (10 μM) abolished the response to S1P in BAC D2 striatopallidal neurons (Kruskal-Wallis ANOVA, p>0.01, n=4; c and d), in agreement with previous work showing that Gpr6 receptors mobilize intracellular Ca2+ stores (D. S. Lim et al., Nature 404, 613 (2000). The Gpr6-dependent elevation of cytosolic Ca2+ seen in striatopallidal cells would be predicted to result in a decrease in threonine 34 phosphorylation of the centrally important regulatory protein DARPP-32, due to activation of the Ca2+ and calmodulin-dependent phosphatase calcineurin (A. Nishi, G. L. Snyder, A. C. Nairn, P. Greengard, J Neurochem 72, 2015-21 (May, 1999)) and/or inhibition of adenylyl cyclase type 5 (AC5; Adcy5) (C. E. Glatt, S. H. Snyder, Nature 361, 536-8 (Feb. 11, 1993); Y. Ishikawa et al., J Biol Chem 267, 13553-7 (Jul. 5, 1992)) This demonstrated the physiological consequence of the S1P-induced increase in cytosolic Ca2+ by measurement of the phosphorylation status of DARPP-32. A decrease in threonine 34 DARPP-32 phosphorylation was seen after 5 minutes of S1P treatment of striatal slices (1.04±0.17 normalized units at 0 minutes; 0.58 normalized units±0.24 at 5 minutes after S1P addition, one-tailed Mann-Whitney test, p=0.05, n=12), consistent with cytosolic Ca2+ elevation in D2 cells (which constitute approximately half of the medium-spiny neurons). These data confirm a prediction based on the BACarray translational profiling, demonstrate a strong and cell-type specific response of striatopallidal neurons to sphingosine 1-phosphate, and identify Gpr6 as a novel and important signaling component of striatopallidal, but not striatonigral, medium spiny neurons.
Example 12
Detection of Pharmacologically Induced Transcriptional Changes
1) Overview
In certain embodiments, responses to genetic, pharmacologic or environmental changes can be analyzed effectively in single cell types using translational profiling and molecular Phenotyping methods described herein. To define uncover novel physiological properties of striatal neurons, possible changes in mRNA expression of MSNs upon pharmacological perturbation of dopaminergic signaling was investigated.
Cocaine, a competitive inhibitor of the dopamine transporter, acts as a psychostimulant by elevating synaptic dopamine levels (M. C. Ritz, R. J. Lamb, S. R. Goldberg, M. J. Kuhar, Science 237, 1219-23 (Sep. 4, 1987); G. Di Chiara, A. Imperato, Proc Natl Acad Sci USA 85, 5274-8 (July, 1988)). Adult mice were treated acutely or chronically with cocaine or saline and used for BACarray profiling of striatonigral (D1) and striatopallidal (D2) MSNs.
2) Experimental Design
Quality control steps taken: three biological replicates were performed for each experiment. Arrays were only used if the Spearman correlation between the other arrays for that experiment was >75% but could be adjusted according to biological variation. Quantitative PCR reactions were performed to validate array results using an independent biological source and amplification methodology. The Experiment included 5 comparisons, with each D1 vs. D2 replicates performed on the same day, as described here in Table 14.





TABLE 14



Experimental Design for Effects of Cocaine






Striatonigral Cells (D1)
Striatopallidal Cells (D2)





No treatment
No treatment


Acute Saline - Replicate 1
Acute Saline - Replicate 1


Acute Cocaine - Replicate 1
Acute Cocaine - Replicate 1


Chronic Saline - Replicate 1
Chronic Saline - Replicate 1


Chronic Cocaine - Replicate 1
Chronic Cocaine - Replicate 1


























3) Animals and Sample Preparation:
Origin of biological sample: seven adult BACarray mice (four female, three male) were pooled for each replicate. Striatal tissue was taken between 7-9 weeks of age. Lines of mice used in each experiment: (Drd1a/eGFP-L10a) and (Drd2/eGFP-L10a).
Manipulation of biological sample: all mice were raised under 12 h dark/12 h light conditions, housed 5 mice per cage, with food and water ad libitum. All mice used in the study were heterozygous for the eGFP-L10a transgene; mice from each line had been crossed to wild-type Swiss-Webster (Taconic Farms) four times. Four heterozygous adult BACarray females and three heterozygous adult BACarray males were used for each study. Mice used for the baseline comparison of D1 versus D2 cells were used directly from the home cage. For all cocaine studies, BACarray mice 7-9 weeks of age were singly housed and intraperitoneally injected at the Rockefeller University Laboratory Animal Research Center (LARC) and were moved to the laboratory approximately 16 h before the final injection. For acute cocaine studies, all mice were injected with 100 μl saline (vehicle) once daily for eight days to habituate the mice to handling On the ninth day mice were injected with a test dose of 20 mg/kg cocaine or 100 μl saline and the striata were harvested 4 h after this test injection. For chronic cocaine studies, mice were injected with 20 mg/kg cocaine or 100 μl saline once daily for fifteen days and the striata were harvested 4 h after the last injection.
Experimental factor values: D1; D2; Acute cocaine D1; Acute saline D1; Acute cocaine D2; Acute saline D2; Chronic cocaine D1; Chronic saline D1; Chronic cocaine D2; Chronic saline D2.
Results:
For the baseline comparison of striatonigral versus striatopallidal cells, a moderated two-tailed paired t-test was performed. The p-value of the moderated t-test was adjusted for multiple hypothesis testing, controlling the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Genes that had an FDR less than 0.1 (10%) and fold change larger than 1.5 were then selected.
For the comparison of striatonigral and striatopallidal BACarray samples versus total brain minus striatum, GeneChip CEL files were imported into Gene spring GX 7.3.1, normalized with the GC-RMA algorithm (BACarray samples and whole brain minus striatum samples separately), and expression values on each chip were further normalized to the expression values of several positive control genes and to a constant value of 0.01. A moderated two-tailed paired t-test was then performed. The p-value of the moderated t-test was adjusted for multiple hypothesis testing, controlling the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Genes that had an FDR less than 0.05 (5%) and fold change larger than 2 were selected
Upon clustering, all cocaine data exhibited a strong effect of the day the samples were prepared. A more complex model that could take into account and adjust for the day effect was utilized. A linear model was adjusted to each gene with factor Treatment (Saline vs Cocaine), Cell type (D1 vs D2), and Day (three different days for acute and chronic experiments). With this analysis, a large number of genes indeed displayed a Day factor. With this model fitted, all the hypotheses were tested (Cocaine vs Saline and its interaction with Cell type in both Acute and Chronic). For assessing differential expression in the comparisons of interest, the moderated t-statistic was used (G. K. Smyth, Stat Appl Genet Mol Biol 3, Article3 (2004)). In this assessment, an empirical Bayes method is used to moderate the standard errors of the estimated log 2-fold changes. This method was particularly useful for the analysis with only three replicates of each condition, as it delivered more stable inference and improved power. The p-value of the moderated t-test was adjusted for multiple hypothesis testing, controlling the false discovery rate (FDR) using the Benjamini-Hochberg procedure. Genes than have FDR less than 0.1 (10%) and fold change larger than 1.4 were selected.
To translate the gene expression data into functional profiles, Gene Ontology (GO) analysis and Pathway analysis was utilized to look for enriched GO terms and Pathways in the set of differentially expressed genes. Analysis of GO annotations was performed using the R-packages GOstats and GOtools. To find those GO terms that were over-represented in the list of genes in question, for each specific term in a given ontology (Biological Process, Molecular Function, Cellular Component), the proportion of genes in the list that falls into the GO term is compared with the proportion in the whole set of genes in the Mouse Genome 430 2.0 array using a conditional hypergeometric test. A conditional instead of the classical hypergeometric test was utilized to address concerns about the hierarchical structure of GO terms (S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007); A. Alexa, J. Rahnenfuhrer, T. Lengauer, Bioinformatics 22, 1600-7 (2006)). The conditional test uses the relationship among GO terms to decorrelate results. GO with p-values <0.1 and with more than one gene were considered. To find overrepresented Pathways, a classical hypergeometric test was utilized to compare the proportion of genes among those that are differentially expressed (Up and Down together) that belong to a certain Pathway, using the whole Mouse Genome 430 2.0 array gene set as the universe for the comparison. A Pathways annotation package available at Bioconductor.org (version 1.16.0) was used. The KEGG Pathways terms in that package were obtained from KEGG:ftp://ftp.genome.ad.jp/pub/kegg/tarfiles/pathway.tar.gz, build release 41.1, Feb. 1, 2007.
From this analysis hundreds of genes were identified whose expression was increased or decreased in each cell type in response to cocaine
Table 15 and Table 16). Various genes whose expression has been reported to be affected by cocaine administration were identified, including: Cartpt (J. Douglass, A. A. McKinzie, P. Couceyro, J Neurosci 15, 2471-81 (March, 1995) (up in acute D1; Table S12); Fosb (B. Hope, B. Kosofsky, S. E. Hyman, E. J. Nestler, Proc Natl Acad Sci USA 89, 5764-8 (Jul. 1, 1992)) (up in acute D1, acute D2, and chronic D1;
Table 15 and Table 16); Homerl (P. R. Brakeman et al., Nature 386, 284-8 (Mar. 20, 1997)) (up in acute D1, acute D2, chronic D1, and chronic D2;
Table 15 and Table 16); Per2 (V. Yuferov et al., Synapse 48, 157-69 (Jun. 15, 2003)) (up in acute D2, chronic D1, chronic D2;
Table 15 and Table 16); Vamp2 (C. A. McClung, E. J. Nestler, Nat Neurosci 6, 1208-15 (November, 2003)) (up in chronic D1; Table S13); Kcnd2 (C. A. McClung, E. J. Nestler, Nat Neurosci 6, 1208-15 (November, 2003)) (up in chronic D1; Table 16); and Zfp64 (C. A. McClung, E. J. Nestler, Nat Neurosci 6, 1208-15 (November, 2003)) (up in acute D2, down in chronic D2;
Table 15 and Table 16).





TABLE 15



Gene translation changes upon acute cocaine treatment










FCH
FCH

Probe
Symbol
GenBank
D1
D2









1459589_at
Cryl1
C85932
6.86
0.37

1459589_at
Vcan
BM251152
3.06
0.97

1423287_at
Cbln1
AA016422
2.88
1.36

1434950_a_at
Armc8
BE995635
2.87
0.78

1422533_at
Cyp51
NM_020010
2.84
0.92

1438752_at
A230058F20Rik
AV327739
2.80
1.67

1428601_at
1700003E16Rik
AK005628
2.69
0.76

1420901_a_at
Hk1
NM_010438
2.38
1.03

1460330_at
Anxa3
AW702161
2.36
1.03

1444137_at
A430108G06Rik
AW120610
2.36
1.03

1443699_at
Ptprk
BE957383
2.31
0.63

1443998_at

BE980823
2.28
1.02

1422904_at
Fmo2
NM_018881
2.26
0.85

1456133_x_at
Itgb5
BB543646
2.15
1.31

1452226_at
Rcc2
AV122997
2.11
1.28

1433836_a_at
8430408G22Rik
AV365503
2.11
0.72

1435872_at
Pim1
BE631223
2.11
2.83

1436387_at
Homer1
BB398124
2.10
2.41

1422134_at
Fosb
NM_008036
2.09
2.49

1451554_a_at
Aph1a
BC012406
2.07
0.89

1431715_a_at
Lig3
AK010719
2.06
0.59

1424393_s_at
Adhfe1
BC026584
1.97
1.04

1437284_at
Fzd1
BB259670
1.97
1.19

1430133_at
Tbc1d8b
AK014817
1.96
1.31

1435458_at
Pim1
AI323550
1.96
2.90

1430600_at
Cmtm5
AK013666
1.96
1.71

1440116_at
D630045J12Rik
AW558117
1.94
1.17

1458284_at
Ptbp1
BM195499
1.93
0.87

1425585_at
Med12
AF071310
1.92
0.99

1418322_at
Crem
AI467599
1.89
1.85

1451411_at
Gprc5b
BC020004
1.88
1.12

1430510_at
B130050I23Rik
AK009699
1.87
1.09

1436682_at
Tmsb10
AW259435
1.87
0.88

1451440_at
Chodl
AF311699
1.87
0.91

1452003_at
Rbm14
BC010294
1.86
0.81

1453787_at
Txndc13
AK015667
1.83
0.91

1432466_a_at
Apoe
AK019319
1.82
1.58

1415851_a_at
Impdh2
M33934
1.81
0.87

1460061_at
Adra1a
AV342748
1.81
1.42

1427465_at
Atp1a2
BC025807
1.81
1.08

1426225_at
Rbp4
U63146
1.80
1.48

1418520_at
Tgoln1
NM_009443
1.80
1.11

1417782_at
Lass4
BB006809
1.79
1.13

1441334_at

BB528600
1.78
0.94

1423278_at
Ptprk
AI893646
1.77
1.21

1421571_a_at
Ly6c
NM_010741
1.77
1.19

1428298_at
1700029G01Rik
AK006490
1.77
0.60

1446147_at

BB436856
1.77
0.80

1437264_at
BC051142
AV278321
1.77
1.22

1455925_at
Prdm8
AV349236
1.76
1.11

1434110_x_at
Mup1
BF322785
1.76
0.72

1422825_at
Cartpt
NM_013732
1.76
1.29

1428054_at
Slc8a2
AF503502
1.76
0.92

1455277_at
Hhip
BB088162
1.75
0.92

1457567_at
Nagk
BM938681
1.75
1.07

1421276_a_at
Dst
NM_134448
1.74
1.42

1434606_at
Erbb3
BF140685
1.74
1.60

1442554_s_at
Kalrn
BG066934
1.74
0.65

1435559_at
Myo6
BB200233
1.73
1.48

1425546_a_at
Trf
AF440692
1.73
1.50

1422053_at
Inhba
NM_008380
1.72
2.10

1420158_s_at
Abcf1
AA408356
1.72
0.83

1427692_a_at
Cask
Y17137
1.72
1.08

1423700_at
Rfc3
BC026795
1.72
1.12

1424805_a_at
1110039B18Rik
BC027046
1.72
1.02

1429628_at
6330407J23Rik
AK012378
1.71
0.93

1437920_at
Epha5
AI854630
1.70
1.29

1417509_at
Rnf19
AF120206
1.70
1.26

1456384_at
Nlgn3
BB308872
1.70
1.03

1440862_at
Tmem181
BB629079
1.70
1.24

1455492_at
B330016D10Rik
BB313577
1.69
1.40

1448502_at
Slc16a7
NM_011391
1.68
1.10

1453583_at
Zzef1
AI466222
1.68
0.80

1420652_at
Ate1
BE309332
1.68
0.71

1420720_at
Nptx2
NM_016789
1.68
1.43

1437147_at
Gabrg2
AV348821
1.67
1.23

1435749_at
Gda
AW911807
1.67
1.11

1437308_s_at
F2r
AV024285
1.67
0.90

1416589_at
Sparc
NM_009242
1.66
1.58

1450708_at
Scg2
NM_009129
1.66
1.59

1425227_a_at
Atp6v0a1
BC001995
1.66
1.19

1433607_at
Cbln4
AV343573
1.66
1.56

1450976_at
Ndrg1
AI987929
1.66
1.25

1429475_at
2810457I06Rik
AK013361
1.66
1.83

1447005_at

BE991564
1.65
0.83

1452754_at
Creld2
AK017880
1.65
1.42

1449411_at
Dscam
NM_031174
1.65
0.99

1428922_at
1200009O22Rik
AK004681
1.65
1.56

1421959_s_at
Adcy3
AF458089
1.64
1.13

1453056_at
Slc16a13
AK005699
1.64
1.08

1455061_a_at
Acaa2
BB718075
1.64
1.66

1439764_s_at
Igf2bp2
BB098431
1.64
1.10

1451699_at
EG668468
BC024416
1.64
0.78

1456825_at
Wdr33
BB482118
1.63
0.98

1438725_at
Thrap1
BB212816
1.63
1.17

1448384_at
Pofut2
BC018194
1.63
1.59

1444690_at
Epha5
BE951744
1.63
1.29

1415885_at
Chgb
NM_007694
1.63
1.54

1442788_at
2600003E23Rik
BB698513
1.63
0.98

1449289_a_at
B2m
BF715219
1.63
1.17

1418132_a_at
D7Wsu128e
BC011313
1.62
0.79

1427539_a_at
Zwint
BC013559
1.62
1.42

1450193_at
Hcn1
NM_010408
1.62
1.45

1457536_at
Gpc5
BB171986
1.62
1.11

1420564_at
Insrr
NM_011832
1.62
0.81

1460583_at
Golt1b
AV349681
1.62
1.08

1425110_at
Sorcs3
AK018111
1.62
1.19

1421535_a_at
Pde4a
NM_019798
1.62
0.75

1452768_at
Tex261
AK013971
1.62
0.97

1438750_at
Atrx
BB425841
1.61
1.05

1443898_at
Bcl7c
BB826733
1.61
1.08

1453531_at
Zfp248
AK012874
1.61
1.01

1418260_at
Hunk
NM_015755
1.61
0.72

1438151_x_at
Zdhhc14
BB544336
1.60
1.26

1425899_a_at
Itsn1
AF132478
1.60
0.89

1418253_a_at
Hspa41
NM_011020
1.60
1.12

1428136_at
Sfrp1
AK008943
1.60
1.08

1429256_at
Gtl2
AU067739
1.60
0.94

1418350_at
Hbegf
L07264
1.60
1.48

1436785_a_at
Sec14l3
AV024133
1.60
0.69

1451289_at
Dcamkl1
AW105916
1.59
1.15

1425725_s_at
Ppp2r5c
BF136532
1.59
1.30

1435568_at
AK129128
AW545855
1.59
1.12

1449151_at
Pctk3
NM_008795
1.59
1.19

1436129_at
4930523C11Rik
AV255799
1.58
0.84

1460293_at
Freq
AU079041
1.58
0.78

1428488_at
Pigk
BB646655
1.58
1.24

1422683_at
Irak1bp1
NM_022986
1.58
0.85

1434856_at
Ankrd44
AV256780
1.58
1.17

1439015_at
Gfra1
AV221299
1.58
1.15

1434987_at
Aldh2
AI462635
1.58
0.79

1436454_x_at
Fen1
BB393998
1.57
0.91

1449799_s_at
Pkp2
AA516617
1.57
2.42

1419557_a_at
Tmem9
NM_025439
1.57
1.28

1423668_at
Zdhhc14
BC021423
1.57
1.75

1427284_a_at
Ttpa
AU019171
1.57
0.95

1434270_at
Nptxr
AV221896
1.57
0.96

1418318_at
Rnfl28
AK004847
1.57
1.16

1434233_at
2610030H06Rik
BB429625
1.57
0.72

1421111_at
Rybp
NM_019743
1.57
1.00

1435109_at
Tmem175
AW123256
1.57
1.12

1457956_at
Camta1
BB777776
1.57
2.10

1418149_at
Chga
NM_007693
1.57
1.66

1428336_at
Agpat4
AK005139
1.56
0.93

1449972_s_at
Zfp97
NM_011765
1.56
0.91

1451611_at
Hrasls3
BC024581
1.56
1.31

1451961_a_at
Mbp
L07509
1.56
1.87

1436094_at
Vgf
BF458396
1.56
1.41

1435904_at
Eif2c3
BB355034
1.55
1.44

1450117_at
Tcf3
NM_009332
1.55
1.36

1441127_at

BB460036
1.54
1.23

1442905_at
Arf4
BB066458
1.54
1.14

1448601_s_at
Msx1
BC016426
1.54
1.08

1436898_at
Sfpq
BI738328
1.54
1.00

1424581_at
Stac2
BC024864
1.54
1.28

1451406_a_at
Pcsk5
BC013068
1.54
1.16

1425415_a_at
Slc1a1
U75214
1.54
1.26

1441165_s_at
Clstn2
AI448973
1.54
1.04

1416371_at
Apod
NM_007470
1.54
1.52

1429463_at
Prkaa2
BB612385
1.54
1.01

1438118_x_at
Vim
AV147875
1.54
0.84

1417502_at
Tspan7
AF052492
1.53
1.09

1435145_at
Cadm2
BB333386
1.53
1.30

1417764_at
Ssr1
BG077348
1.53
0.97

1436805_at
2810457I06Rik
BG076317
1.53
1.51

1452429_s_at
Abcf1
BF236176
1.53
1.34

1423643_at
Ddx39
BC020134
1.53
1.44

1425536_at
Stx3
D29797
1.53
0.73

1451386_at
Blvrb
BC027279
1.53
1.19

1456271_at
Gm944
BB039066
1.53
0.90

1434199_at
Gm323
BM898903
1.53
1.21

1450983_at
Akap8
BG069776
1.52
1.11

1452905_at
Gtl2
AV015833
1.52
0.67

1449381_a_at
Pacsin1
BI731319
1.52
1.08

1445268_at

AW550459
1.52
1.09

1425568_a_at
Tmem33
BC005562
1.51
1.05

1450699_at
Selenbp1
NM_009150
1.51
1.35

1418104_at
Nrip3
NM_020610
1.51
1.90

1448050_s_at
Map4k4
BF450398
1.51
2.05

1452835_a_at
Polrmt
AK003792
1.51
1.44

1423070_at
Rpl21
BG922742
1.51
1.13

1430798_x_at
Mrpl15
AV306676
1.51
1.76

1439380_x_at
Gtl2
BB093563
1.51
0.91

1460170_at
Ext2
NM_010163
1.51
1.17

1450965_at
Tex261
BF181445
1.51
1.24

1456389_at
Zeb2
BB244754
1.51
0.90

1421374_a_at
Fxyd1
NM_019503
1.51
0.92

1450351_a_at
Clip1
NM_019765
1.51
1.10

1424133_at
Tmem98
BC011208
1.50
1.24

1434585_at
Tulp4
BB667130
1.50
1.45

1429591_at
Tacc1
BE862546
1.50
1.45

1418816_at
Chmp1b
BG073376
1.50
1.42

1424950_at
Sox9
BC024958
1.50
0.97

1460743_at
Tigd5
BB553398
1.49
1.22

1439559_at
1700040D17Rik
AV260432
1.49
1.08

1460331_at
Tm9sf2
BB747462
1.49
1.40

1435703_at

AW045947
1.49
1.64

1436325_at
Rora
BB306272
1.49
0.76

1453334_at
B230216N24Rik
AK021003
1.49
1.60

1423859_a_at
Ptgds
AB006361
1.49
1.06

1438706_at
6430517E21Rik
BB309608
1.49
1.27

1415806_at
Plat
NM_008872
1.49
1.56

1429055_at
4930506M07Rik
BB559293
1.48
1.10

1431755_a_at
Ccdc49
BC021478
1.48
1.05

1437495_at
Mbtps2
AV272026
1.48
1.16

1421487_a_at
Nck1
NM_010878
1.48
1.40

1426781_at
Tyw1
BF021045
1.48
1.05

1426852_x_at
Nov
X96585
1.48
1.53

1436137_at
Slc6a17
AV340147
1.48
1.17

1447759_x_at
Ccdc22
BB345575
1.48
0.73

1418045_at
Inpp1
NM_008384
1.48
1.52

1448242_at
Sec61a1
BC003707
1.48
1.20

1424861_at
Zbtb24
BM200153
1.48
0.86

1437142_a_at
Pigo
BB546713
1.48
1.14

1423796_at
Sfpq
AY034062
1.47
1.40

1458622_at
Ntrk2
AW121243
1.47
1.29

1416032_at
Tmem109
AW494906
1.47
0.95

1455476_a_at
Gse1
BE686504
1.47
1.55

1426311_s_at
Zdhhc5
AV241525
1.47
1.51

1418456_a_at
Cxcl14
AF252873
1.47
0.92

1455965_at
Adamts4
BG064671
1.47
1.26

1455993_at
Odz4
BG071076
1.47
1.25

1431385_a_at
Mbtps1
AK002809
1.47
1.09

1450172_at
Pknox1
AA409923
1.47
1.77

1415901_at
Plod3
NM_011962
1.47
1.16

1420838_at
Ntrk2
AK018789
1.47
1.61

1455908_a_at
Scpep1
AV102733
1.47
0.82

1418402_at
Adam19
NM_009616
1.46
1.03

1434505_a_at
6430548M08Rik
BF465109
1.46
0.87

1438059_at
1700011I03Rik
BM116248
1.46
0.80

1434235_at
Slc20a2
BB765719
1.46
1.37

1420845_at
Mrps2
AV031454
1.46
1.28

1423566_a_at
Hsp110
BI499717
1.46
1.18

1436919_at
Trp53i11
BQ175031
1.46
0.88

1428403_at
2410025L10Rik
AV330483
1.46
1.43

1430568_at
Zc3h13
AW539538
1.46
1.87

1456620_at
Gnptab
BE944732
1.46
1.18

1458384_at

AW491514
1.46
0.85

1437879_at
Alg10b
BG071776
1.45
0.97

1416702_at
Serpini1
NM_009250
1.45
1.63

1451352_s_at
Mta3
BC022124
1.45
1.22

1451369_at
Commd5
BC025891
1.45
0.67

1427994_at
Cd300lf
BM230330
1.45
0.99

1460098_at
LOC629517
BB363968
1.45
1.46

1439408_a_at
Pparbp
AV100992
1.45
1.17

1449286_at
Ntng1
NM_030699
1.45
1.32

1452624_at
Ctnna2
BM900077
1.45
1.78

1437092_at
Clip4
AV042829
1.45
1.03

1437966_at
Prrt3
AV329802
1.45
1.41

1449164_at
Cd68
BC021637
1.45
1.23

1456727_a_at
Csnk1d
AW413676
1.44
0.88

1435993_at
4930470G03Rik
BB027219
1.44
1.75

1416588_at
Ptprn
NM_008985
1.44
1.27

1419646_a_at
Mbp
NM_010777
1.44
1.52

1449730_s_at
Fzd3
AU020229
1.44
1.08

1452203_at
Obfc2a
AV313559
1.44
1.42

1426620_at
Chst10
BB549997
1.44
1.34

1427021_s_at
Eif3s5
AK002778
1.44
0.98

1423423_at
Pdia3
BF319868
1.43
1.11

1455009_at
Cpd
AW550842
1.43
1.35

1451071_a_at
Atp1a1
BC025618
1.43
0.94

1418331_at
1110031I02Rik
NM_025402
1.43
1.05

1448603_at
Srpk2
NM_009274
1.43
1.37

1421816_at
Gsr
AK019177
1.43
1.23

1434102_at
Nfib
BI687652
1.43
0.86

1419938_s_at
Arhgef17
AW558066
1.43
1.21

1455917_at
Ntrk3
BB456963
1.43
0.93

1441880_x_at
U2afl14
BB009770
1.43
1.19

1448397_at
Gjb6
BC016507
1.43
1.62

1457467_at
Pknox1
BB362579
1.43
1.03

1436097_x_at
Arhgap9
BB327418
1.43
0.44

1420419_a_at
Otof
NM_031875
1.43
0.97

1438458_a_at
Sfpq
BF224766
1.42
1.49

1424781_at
Reep3
AK005026
1.42
0.97

1460205_at
Dcakd
NM_026551
1.42
1.05

1421491_a_at
Tmem49
NM_029478
1.42
1.60

1449405_at
Tns1
NM_027884
1.42
1.31

1455122_at
Ptchd2
AI666776
1.42
1.01

1456379_x_at
Dner
BB038556
1.42
1.27

1421360_at
Inpp4a
NM_030266
1.42
1.27

1450010_at
Hsd17b12
AK012103
1.42
1.92

1436057_at
Gtl2
BM117428
1.42
1.23

1449885_at
Tmem47
NM_138751
1.42
2.12

1426280_at
AV249152
AF424701
1.42
1.13

1438165_x_at
Vat1
BB559097
1.42
0.88

1440258_at
Kcnq2
BE951512
1.41
1.35

1448113_at
Stmn1
BC010581
1.41
0.93

1428793_at
Slc36a1
AK017918
1.41
1.43

1438957_x_at
Cds2
BB027654
1.41
1.19

1438975_x_at
Zdhhc14
AV361868
1.41
1.49

1448957_at
Rbpsuh
NM_009035
1.41
1.14

1429257_at
Gtl2
AU067739
1.41
0.81

1447774_x_at
5730469M10Rik
AV332575
1.41
1.14

1426767_at
3230401M21Rik
AK014328
1.41
1.87

1428586_at
Tmem41b
BB823331
1.41
1.00

1441737_s_at
Rassf1
BB757103
1.41
0.90

1450872_s_at
Lipa
AI596237
1.41
1.18

1417946_at
Abhd3
NM_134130
1.41
1.26

1439776_at
Ogfod1
BE956398
1.41
1.31

1453581_at
Cep170
BB361436
1.41
1.30

1452232_at
Galnt7
AV302406
1.41
1.55

1440143_at
Pigz
AI413722
1.41
0.94

1460327_at
Gpr88
BE947345
1.40
1.40

1423294_at
Mest
AW555393
1.40
1.79

1445605_s_at
4921533L14Rik
AI835491
1.40
1.06

1450268_at
Fign
NM_021716
1.40
1.68

1417605_s_at
Camk1
NM_133926
1.40
0.94

1423950_at
Kcnab3
BC017518
1.40
1.38

1425301_at
Ncam2
AF001287
1.40
0.87

1417149_at
P4ha2
NM_011031
1.40
1.53

1420969_at
Btbd14b
NM_025788
1.39
0.69

1448673_at
Pvrl3
NM_021495
1.39
0.67

1451718_at
Plp1
BB768495
1.38
1.44

1423826_at
Noc41
BC024616
1.38
1.45

1437614_x_at
Zdhhc14
AV223474
1.38
1.46

1425474_a_at
Vps39
BC007479
1.38
1.61

1430294_at
Ssbp1
AK013425
1.38
1.43

1448216_at
Syngr3
NM_011522
1.38
1.45

1423192_at
Pspc1
BB590675
1.37
1.84

1456783_at
9330107J05Rik
BB075402
1.37
1.44

1426449_a_at
Pja1
BM199789
1.37
1.45

1437857_at
Dpy19l3
AV367203
1.37
1.45

1418349_at
Hbegf
L07264
1.37
1.62

1444327_at
Edd1
BF224640
1.37
1.63

1431802_a_at
D5Wsu178e
AK014530
1.36
1.47

1435553_at
Pdzd2
AV376136
1.36
1.83

1436201_x_at
Mbp
BB761376
1.36
1.51

1426837_at
Metap1
BG064851
1.36
1.77

1417110_at
Man1a
BB070019
1.35
1.45

1444661_at
Gpr26
BB247791
1.35
1.96

1447672_x_at
Pskh1
BB102342
1.35
0.64

1417954_at
Sst
NM_009215
1.35
1.41

1444614_x_at
6430537H07Rik
AV258949
1.35
0.33

1435456_at
Ttc28
AW552254
1.35
1.44

1451726_at
Mtmr6
BC020019
1.34
1.66

1421201_a_at
Tro
AF241244
1.34
1.50

1442565_at
Pcdh10
BB174272
1.34
0.70

1416271_at
Perp
NM_022032
1.34
0.70

1425656_a_at
Baiap2
AF390178
1.34
1.41

1422629_s_at
Shroom3
NM_015756
1.34
1.50

1435158_at
Rbm12b
BB796313
1.34
1.43

1434192_at
Zzef1
BQ175699
1.34
1.46

1454608_x_at
Ttr
BG141874
1.33
0.16

1425329_a_at
Cyb5r3
AF332060
1.33
1.76

1439406_x_at
Fars2
BB530332
1.33
0.71

1419873_s_at
Csflr
AI323359
1.33
1.69

1454768_at
Kcnfl
AV337635
1.33
1.40

1432000_a_at
Dedd
AK006814
1.32
1.51

1436444_at
6030405A18Rik
AV319257
1.32
1.53

1433748_at
Zdhhc18
BG073303
1.32
1.45

1418941_at
Pcdhb22
NM_053147
1.32
1.54

1437472_at
Unc13a
BB429098
1.31
1.43

1430193_at
Casc5
BF021255
1.31
0.55

1438619_x_at
Zdhhc14
BB318221
1.31
1.44

1456070_at
5430405N12Rik
AI507538
1.31
1.46

1450905_at
Plxnc1
BB476707
1.31
1.54

1451680_at
Srxn1
BC011325
1.31
1.58

1425517_s_at
Ogt
AF363030
1.31
1.44

1427893_a_at
Pmvk
BI713896
1.30
1.41

1417279_at
Itpr1
NM_010585
1.30
1.49

1422621_at
Ranbp2
BM507707
1.30
1.54

1436231_at
AU067665
AU067665
1.30
1.47

1448883_at
Lgmn
NM_011175
1.30
1.46

1456833_at
Gpr17
BB353220
1.29
1.56

1436611_at
Slc39a12
AW046938
1.29
2.04

1422018_at
Hivep2
NM_010437
1.29
1.56

1437067_at
Phtf2
BM228625
1.29
1.43

1439289_s_at
0710005I19Rik
BB197516
1.29
0.62

1434891_at
Ptgfrn
AV253087
1.28
1.43

1416445_at
2810405J04Rik
NM_133747
1.28
1.68

1438078_at

BF730550
1.28
1.48

1448139_at
Mlc1
NM_133241
1.28
2.12

1416262_at
Tmem19
AK018383
1.28
1.53

1418540_a_at
Ptpre
U35368
1.28
1.43

1416178_a_at
Plekhb1
NM_013746
1.28
1.47

1451163_at
Tinf2
AF214013
1.28
1.57

1451499_at
Cadps2
AF000969
1.27
0.60

1416933_at
Por
NM_008898
1.27
1.48

1424112_at
Igf2r
BG092290
1.27
1.59

1451133_s_at
Tmem168
BC007160
1.27
1.45

1449209_a_at
Rdh11
AB030503
1.27
1.54

1416749_at
Htra1
NM_019564
1.27
1.85

1431711_a_at
9030409G11Rik
AK018497
1.27
1.67

1440779_s_at
Slc5a9
BB502156
1.27
0.63

1418007_at
1810007M14Rik
BM932567
1.27
1.42

1428834_at
Dusp4
AK012530
1.27
1.55

1452757_s_at
Hba-a1
AK011116
1.27
1.53

1419063_at
Ugt8a
NM_011674
1.27
1.47

1456110_at
Ankrd11
BB320513
1.27
1.46

1418626_a_at
Clu
NM_013492
1.27
1.70

1428031_at
Mchr1
BE647763
1.26
1.49

1454654_at
Dirc2
BG069395
1.26
1.47

1423574_s_at
Srd5a2l
BB206480
1.26
1.49

1450971_at
Gadd45b
AK010420
1.26
1.98

1452377_at
Mll1
AK017541
1.26
1.57

1458979_at

BB332816
1.26
1.54

1444307_at

AW491448
1.26
2.53

1420502_at
Sat1
NM_009121
1.26
1.47

1439041_at
Slc39a10
BM239325
1.26
1.56

1428387_at
Acsl3
AK012088
1.25
1.55

1458842_at

AV340888
1.25
1.40

1424482_at
Arhgef7
AF343877
1.25
1.46

1418169_at
Zcchc14
BB223737
1.25
1.45

1427931_s_at
Pdxk
BG063905
1.25
1.60

1429183_at
Pkp2
AK005020
1.25
1.57

1434510_at
Papss2
BF780807
1.25
1.68

1424098_at
Elovl7
BC005602
1.25
1.41

1415857_at
Emb
BG064842
1.25
1.61

1418539_a_at
Ptpre
U35368
1.24
1.45

1417988_at
Resp18
NM_009049
1.24
1.60

1444226_at
Foxo3a
W07885
1.24
1.81

1450326_at
Shc3
NM_009167
1.24
1.44

1438402_at
9630050M13Rik
BB131315
1.24
1.51

1439279_at
3110007F17Rik
AV339304
1.24
1.45

1415743_at
Hdac5
NM_010412
1.24
1.42

1417947_at
Pcna
BC010343
1.24
1.53

1423795_at
Sfpq
AY034062
1.24
1.53

1428112_at
Armet
AK014338
1.24
1.46

1437159_at
Eif2c5
AW553762
1.24
1.55

1429517_at
Zfyve20
BC017622
1.23
2.63

1448460_at
Acvr1
NM_007394
1.23
1.44

1453109_at
Arsk
AK013194
1.23
0.67

1421010_at
Mobp
NM_008614
1.23
1.78

1434761_at
Lrrtm3
BM224801
1.23
1.85

1449773_s_at
Gadd45b
AI323528
1.23
1.55

1437633_at
Ankrd11
BB320513
1.23
1.46

1421190_at
Gabrb3
BB367779
1.23
1.55

1434595_at
Trim9
BQ174474
1.23
1.69

1459737_s_at
Ttr
AA408768
1.23
0.18

1428350_at
2310061F22Rik
AV091287
1.23
1.70

1429683_at
5830472M02Rik
BG094398
1.22
1.51

1457125_at

AV266261
1.22
1.86

1425351_at
Srxn1
BC011325
1.22
1.46

1435245_at
Gls2
AI195532
1.22
1.43

1431645_a_at
Gdi2
AK013104
1.22
1.52

1434759_at
Lrrtm3
BM224801
1.22
1.52

1460206_at
Grasp
NM_019518
1.22
1.67

1437880_at
Lbxcor1
AV273001
1.22
0.67

1428765_at
Gtl2
AV015833
1.21
0.66

1451895_a_at
Dhcr24
BC004738
1.21
1.44

1428781_at
Dmkn
BI452905
1.21
1.80

1417238_at
Ewsr1
NM_007968
1.21
1.85

1425396_a_at
Lck
BC011474
1.21
0.62

1438116_x_at
Slc9a3r1
BB805362
1.21
1.70

1421948_a_at
Ccdc123
BB042564
1.20
1.50

1438194_at
2900019G14Rik
AW488243
1.20
1.42

1439333_at
Kcnv1
BB078927
1.20
1.60

1437573_at

BF018351
1.20
0.61

1455291_s_at
Znrf2
BG071922
1.20
1.48

1418195_at
Galnt10
BG965198
1.20
1.61

1425977_a_at
Slk
AF112855
1.20
1.85

1427930_at
Pdxk
BG063905
1.20
1.82

1421199_at
Dlg2
BB622767
1.20
1.47

1442400_at
Prickle1
BF472052
1.19
0.61

1427077_a_at
Ap2b1
AV271093
1.19
1.52

1436501_at
Mtus1
BB747681
1.19
1.45

1456388_at
Atp11a
AV378604
1.19
1.55

1427304_at
Vps18
AW541109
1.19
1.52

1427889_at
Spna2
AK011566
1.19
1.73

1448353_x_at
Rpn1
NM_133933
1.19
1.42

1450538_s_at
Mcpt9
AY007568
1.19
0.56

1451078_at
2510039O18Rik
BC012878
1.19
1.54

1435947_at
2810455D13Rik
BQ175669
1.18
1.44

1428908_at
2600011C06Rik
BG228787
1.18
1.69

1423553_at
Dnajb3
AK005688
1.18
0.71

1434763_at
Tmem142b
BF457736
1.18
1.45

1444509_at

AI847347
1.18
1.49

1452094_at
P4ha1
AI314028
1.18
1.68

1427308_at
Dab1
BB644109
1.18
1.57

1430700_a_at
Pla2g7
AK005158
1.18
2.25

1436329_at
Egr3
AV346607
1.18
1.43

1438565_at
A830010M20Rik
BB464727
1.18
1.88

1430834_at

BB359379
1.18
0.66

1436811_at
Kctd3
AV232970
1.17
1.40

1426278_at
Ifi27
AY090098
1.17
1.52

1420491_at
Eif2s1
BC016497
1.17
1.50

1426910_at
Pawr
BB398886
1.17
0.68

1455110_at
Gabpb2
AW536205
1.17
1.61

1460043_at

BB357628
1.17
2.10

1424734_at
Rasgrf1
AF169826
1.17
1.45

1453796_a_at
Ergic2
AK016275
1.17
1.51

1434893_at
Atp1a2
AI845177
1.17
1.59

1420402_at
Atp2b2
NM_009723
1.16
1.56

1448154_at
Ndrg2
NM_013864
1.16
1.92

1420022_s_at
Suz12
AU022339
1.16
1.48

1424077_at
Gdpd1
AK016023
1.16
1.95

1424869_at
Dhrs7b
BC003479
1.16
1.43

1457456_at
Ttc9b
AI481735
1.16
0.68

1460716_a_at
Cbfb
NM_022309
1.16
0.71

1427593_at
Trim8
BB620112
1.16
1.52

1426959_at
Bdh1
BF322712
1.16
1.88

1452034_at
Prepl
BB005298
1.16
1.45

1426381_at
Pprc1
BM199989
1.15
1.54

1421884_at
Sos1
BB471450
1.15
1.43

1448908_at
Ppap2b
NM_080555
1.15
1.65

1455698_at
Tloc1
BQ175470
1.15
1.42

1428168_at
Mpzl1
AK003513
1.15
1.51

1426770_at
Pex5
AJ416473
1.15
1.69

1428540_at
3321401G04Rik
BE944524
1.15
1.41

1455632_at
Gnb5
BE446953
1.15
2.01

1424684_at
Rab5c
BC023027
1.15
0.69

1452829_at
Cad
AK010453
1.15
1.41

1455628_at
Epb4.1l4b
BB317849
1.14
1.46

1430990_s_at
Mrpl44
AK019986
1.14
1.50

1430066_at
4930589M24Rik
AK014044
1.14
1.49

1434360_s_at
Ptprg
AI596632
1.14
1.58

1415964_at
Scd1
NM_009127
1.14
1.55

1439254_at
Akap13
BE981392
1.14
0.64

1425617_at
Dhx9
U91922
1.14
1.67

1450777_at
Xrn2
NM_011917
1.14
1.46

1417170_at
Lztfl1
NM_033322
1.14
1.52

1439324_at
D230004J03Rik
BB282778
1.14
0.66

1454915_at
Rab3gap2
BB032142
1.14
1.71

1424146_at
Gpr37l1
AB016602
1.14
1.58

1416562_at
Gad1
AF326547
1.14
1.56

1422779_at
Smpd3
NM_021491
1.14
1.63

1455468_at

BB768758
1.14
1.43

1427016_at
4932438A13Rik
BC027125
1.14
1.41

1427138_at
0610010D24Rik
AW556861
1.14
1.41

1423475_at
Cnnm2
BB278418
1.13
1.57

1423479_at
Nol11
AK005123
1.13
1.53

1451285_at
Fus
AF224264
1.13
2.12

1456312_x_at
Gsn
AV224521
1.13
2.63

1436515_at
E030004N02Rik
BB529913
1.13
1.44

1442714_at
D130037M23Rik
BB322545
1.13
1.93

1454811_a_at
Serinc1
AV026664
1.13
1.55

1420262_at

AV291437
1.13
0.67

1454890_at
Amot
BG067039
1.13
1.40

1454784_at
Hs3st2
AV340742
1.13
1.44

1426809_at
C430004E15Rik
BF582414
1.12
1.83

1453122_at
4921533L14Rik
AK019549
1.12
1.44

1416794_at
Arl6ip2
NM_019717
1.12
1.61

1458417_at
Jmjd1b
BB712040
1.12
1.56

1437295_at
Pkn2
AI507382
1.12
1.57

1417475_at
Atp13a1
NM_133224
1.12
1.48

1455459_at
Prdm15
BB213846
1.12
1.44

1417602_at
Per2
AF035830
1.12
1.88

1426991_at
1810048J11Rik
AV273819
1.12
1.40

1441921_x_at
Esrrb
BB386239
1.12
0.62

1450480_a_at
Gprk6
AF040749
1.12
1.42

1457791_at
B830008H07Rik
BB333194
1.12
1.62

1426897_at
Rcc2
AV122997
1.11
1.42

1424161_at
Ddx27
BC011321
1.11
1.54

1429514_at
Ppap2b
AW111876
1.11
1.60

1453851_a_at
Gadd45g
AK007410
1.11
1.41

1424373_at
Armcx3
AK004598
1.11
1.52

1435890_at
AW228836
BB795103
1.11
1.54

1457664_x_at
C2
AV227574
1.11
1.45

1427888_a_at
Spna2
AK011566
1.11
1.45

1425713_a_at
Rnf146
BC019182
1.11
1.45

1423269_a_at
Nedd41
BB663717
1.10
1.66

1451308_at
Elovl4
BB829575
1.10
1.44

1426600_at
Slc2a1
BM209618
1.10
2.23

1421845_at
Golph3
AV174110
1.10
1.49

1430034_at
Cct4
BE648588
1.10
1.66

1417775_at
Rpo1-4
BB729239
1.10
1.43

1453195_at
Sdccag3
BF319466
1.10
1.45

1455395_at
Oxsm
BB446073
1.10
0.70

1442764_at
Suv420h1
BE456272
1.09
1.99

1426542_at
Endod1
BF168366
1.09
1.41

1455023_at
N28178
BB171181
1.09
1.43

1451351_at
Ttc13
BC017545
1.09
1.43

1421100_a_at
Dab1
NM_010014
1.09
1.46

1424036_at
Prpf6
BC014869
1.09
1.43

1440397_at

BB464523
1.09
1.48

1435717_at
4833428C12Rik
AV250971
1.09
1.48

1451069_at
Pim3
BC017621
1.09
1.50

1450135_at
Fzd3
AU043193
1.09
1.42

1420978_at
Nrf1
NM_010938
1.09
1.45

1450384_at
Bace1
AF200346
1.09
1.92

1455194_at
Mapk8ip2
AW536912
1.09
1.57

1419283_s_at
Tns1
NM_027884
1.09
1.58

1445415_at
EG328191
AW048415
1.09
0.70

1423449_a_at
Actn4
BM234779
1.09
1.46

1449556_at
H2-T23
NM_010398
1.09
1.63

1442094_at
Ing5
BB470038
1.08
0.71

1426434_at
Tmem43
AI747289
1.08
1.68

1434448_at
Txlna
BG070463
1.08
1.47

1438459_x_at
Sfpq
BF224766
1.08
1.43

1421342_at
Kcns2
NM_008436
1.08
1.45

1426441_at
Slc11a2
BG065264
1.08
1.44

1447326_s_at
Zmym3
AW122925
1.08
1.55

1450399_at
Psen1
L42177
1.08
1.74

1428352_at
Arrdc2
AW542672
1.08
1.41

1419385_a_at
Ubqln1
NM_026842
1.08
1.46

1436789_at
Ccnjl
BM120177
1.08
1.56

1417694_at
Gab1
NM_021356
1.08
1.50

1435390_at
Exod1
BG067990
1.08
1.49

1456570_at
Epb4.1l4b
BB801576
1.08
1.66

1452197_at
Smc4
AV172948
1.08
1.70

1444468_at
Paqr8
AV328983
1.07
1.66

1449026_at
Ifnar1
NM_010508
1.07
1.57

1421016_at
Ighmbp2
AW259474
1.07
1.41

1427579_at
Rhbdl3
BE653576
1.07
1.58

1416783_at
Tac1
NM_009311
1.07
1.56

1451446_at
Antxr1
AF378762
1.07
1.60

1436787_x_at
Sec14l3
AV024133
1.07
0.53

1448235_s_at
Hmgb1
BF166000
1.07
0.66

1455763_at
Rnf41
BB753958
1.07
0.66

1452524_a_at
Elavl3
U29149
1.07
1.51

1436353_at
A230046K03Rik
AU018634
1.06
1.50

1458584_at
4832406H04Rik
BE948494
1.06
1.56

1423678_at
BC017643
BC017643
1.06
1.59

1432029_a_at
Smap1
AK006817
1.06
1.54

1448591_at
Ctss
NM_021281
1.06
1.81

1437864_at
Adipor2
BE632137
1.06
1.72

1426466_s_at
Rps6kl1
BB311088
1.06
1.49

1431422_a_at
Dusp14
AK009744
1.06
1.74

1455913_x_at
Ttr
AV152953
1.06
0.14

1442823_at

BB536648
1.05
1.79

1424613_at
Gprc5b
BC020004
1.05
1.59

1442073_at

BQ266693
1.05
1.67

1452441_at
Phf3
AW910425
1.05
0.68

1422902_s_at
Mgea5
NM_023799
1.05
1.47

1450748_at
Smpd3
NM_021491
1.05
1.42

1426590_at
Gfm2
BB497484
1.05
1.48

1442257_at
Cdh6
BI134319
1.05
1.70

1431056_a_at
Lpl
AK017272
1.04
1.43

1417749_a_at
Tjp1
NM_009386
1.04
1.53

1460694_s_at
Svil
BM203457
1.04
1.76

1426368_at
Rin2
AK014548
1.04
1.47

1450875_at
Gpr37
BQ175510
1.04
1.68

1419115_at
Alg14
AA198774
1.04
0.61

1427189_at
Arih1
BB362210
1.04
1.46

1435621_at
Mlstd1
BQ175346
1.04
1.45

1437105_at
Jarid1a
BM246184
1.04
1.45

1450108_at
Kif1a
NM_008440
1.04
1.51

1454844_at
Mchr1
AW049955
1.04
1.47

1418754_at
Adcy8
NM_009623
1.04
1.41

1451115_at
Pias3
BC023128
1.04
0.61

1419058_at
Polr1e
NM_022811
1.04
1.56

1432452_at
1700054O19Rik
AK006789
1.04
0.70

1435444_at
Atf6
AV270913
1.04
1.42

1431717_at
3526401B18Rik
AK014386
1.03
1.63

1452295_at
Tmepai
AV291712
1.03
1.46

1431035_at
Daam1
AW988556
1.03
1.67

1434791_at
Atp6v0a2
BM229554
1.03
1.50

1459703_at
D7Ertd443e
BG067408
1.03
0.55

1422688_a_at
Nras
BB018528
1.03
1.61

1424532_at
Ylpm1
AB033168
1.03
1.60

1448439_at
D17Wsu104e
NM_080837
1.03
1.59

1416541_at
Clpb
NM_009191
1.03
1.43

1434548_at
Serinc3
BM244064
1.03
1.44

1435590_at
D430047L21Rik
AV325177
1.03
1.47

1425803_a_at
Mbd2
AF072245
1.03
1.41

1437788_at
Sp6
BB473680
1.03
0.55

1455945_at
Zfp87
AV282347
1.03
1.87

1441323_at
Qpctl
AV370587
1.03
1.61

1417356_at
Peg3
AB003040
1.02
1.44

1424111_at
Igf2r
BG092290
1.02
1.66

1433599_at
Baz1a
BM239162
1.02
3.59

1416550_at
Slc35b4
BB320416
1.02
1.70

1434506_at
Arid2
AV273623
1.02
1.43

1450850_at
Vil2
BM119387
1.02
1.64

1428623_at
Plxna1
AK011193
1.02
1.43

1450213_at
Pde7b
NM_013875
1.02
1.46

1454995_at
Ddah1
AW556888
1.02
1.57

1420011_s_at
Xbp1
C77390
1.02
1.48

1435295_at
Dopey1
BG075614
1.01
1.42

1437467_at
Alcam
AV315205
1.01
0.71

1454680_at
D5Ertd579e
BG073020
1.01
1.41

1420619_a_at
Aes
NM_010347
1.01
0.70

1452690_at
Khsrp
BQ174458
1.01
0.58

1460733_at
AA407659
BB390422
1.01
1.41

1417174_at
1810021J13Rik
NM_025464
1.01
1.42

1459544_at

BB180990
1.01
1.44

1438638_x_at
1700027J05Rik
BB195788
1.01
0.51

1438887_a_at
Gmcl1
BM239632
1.01
1.64

1423531_a_at
Hnrpa1
BI663320
1.01
1.71

1434143_at
BC060631
BQ266886
1.01
1.42

1435580_at
C230081A13Rik
AW553275
1.01
1.46

1420984_at
Pctp
AF114437
1.00
0.50

1431080_at
1700021K10Rik
BB611374
1.00
1.51

1437626_at
Zfp36l2
BB031791
1.00
1.43

1427135_at
Sfrs12
AV012790
1.00
0.49

1454845_x_at
Mchr1
AW049955
1.00
1.43

1424471_at
Rapgef3
BC020532
1.00
1.62

1433776_at
Lhfp
AV149705
1.00
1.73

1417566_at
Abhd5
AK007421
1.00
1.46

1432435_s_at
C030004A17Rik
AK012261
1.00
1.47

1432161_a_at
Ptar1
AK006988
1.00
0.63

1448954_at
Nrip3
NM_020610
1.00
1.53

1416620_at
Smarcal1
NM_018817
0.99
1.43

1425844_a_at
Rngtt
AF034568
0.99
1.67

1437858_at
Dpy19l3
AV367203
0.99
1.51

1451191_at
Crabp2
BC018397
0.99
1.58

1450407_a_at
Anp32a
AF022957
0.99
0.63

1416527_at
Rab32
NM_026405
0.99
1.44

1426712_at
Slc6a15
BB129409
0.99
1.40

1415836_at
Aldh18a1
NM_019698
0.99
1.56

1423301_at
Copb1
BF147382
0.99
1.46

1421471_at
Npy1r
NM_010934
0.99
1.56

1435171_at
Tbl1xr1
BB667085
0.99
1.50

1417153_at
Btbd14a
NM_026495
0.99
0.63

1420922_at
Usp9x
AW107303
0.99
1.45

1441300_at
Kcnf1
BB275623
0.98
1.52

1452415_at
Actn1
BC003232
0.98
0.67

1455980_a_at
Gas2l3
BB770972
0.98
1.41

1460735_at
Svil
BM203457
0.98
1.77

1424290_at
Osgin2
BB817847
0.98
1.75

1443793_x_at
Ints1
BB444598
0.98
2.51

1453264_at
Marveld3
AK007346
0.98
0.71

1416432_at
Pfkfb3
NM_133232
0.98
1.54

1422469_at
Tbk1
NM_019786
0.98
1.41

1423419_at
Lig3
BM248530
0.98
1.48

1424876_s_at
Spg20
BB040507
0.98
1.43

1429684_at
5830472M02Rik
BG094398
0.98
2.11

1439163_at
Zbtb16
BQ174973
0.98
1.44

1451142_at
Exoc1
BC024678
0.97
1.43

1439909_at
Zfp521
BE133660
0.97
1.47

1454944_at
Hic2
BE648070
0.97
1.46

1423569_at
Gatm
AW108522
0.97
1.52

1438511_a_at
1190002H23Rik
BB408123
0.97
1.56

1455214_at
Mitf
BB763517
0.97
1.53

1425007_at
Zfp566
BC019790
0.97
1.50

1454647_at
Acad11
BQ031255
0.97
1.45

1453527_a_at
Neurl
AK010787
0.97
0.65

1421844_at
Il1rap
BE285634
0.97
1.53

1443773_at
Ylpm1
AI851834
0.97
1.77

1433649_at
Aof1
BM243793
0.96
1.41

1428560_at
Xpo5
AK012248
0.96
1.67

1431066_at
Fut11
BB626220
0.96
1.61

1423311_s_at
Tpbg
BQ177165
0.96
1.56

1440251_s_at
Zfp64
BE947768
0.96
1.45

1437244_at
Gas2l3
BB770972
0.96
1.79

1437745_at
Chd7
BB127276
0.96
1.45

1418937_at
Dio2
AF177196
0.96
1.53

1437247_at
Fosl2
BM245170
0.96
1.43

1456233_at

BB080923
0.96
1.46

1426733_at
Itpk1
AW552407
0.96
1.56

1452312_at
2810002D19Rik
BC022909
0.96
1.75

1454070_a_at
Ddhd1
AK014975
0.96
1.71

1422502_at
Parp1
AF126717
0.96
1.50

1425987_a_at
Kcnma1
L16912
0.96
1.43

1434737_at
Obfc1
AV000765
0.95
1.44

1425529_s_at
D19Wsu162e
BC026369
0.95
1.48

1415685_at
Mtif2
NM_133767
0.95
1.52

1420669_at
Arnt2
AI428888
0.95
1.49

1452292_at
Ap2b1
AV271093
0.95
1.44

1442754_at
C030013G03Rik
BE692283
0.95
2.35

1420003_at
Nsun5
C80273
0.95
1.48

1426979_at
Mlxip
AF265663
0.95
1.42

1423247_at
Txndc4
BI100077
0.94
1.52

1419669_at
Prtn3
U97073
0.94
0.64

1420886_a_at
Xbp1
NM_013842
0.94
1.48

1416505_at
Nr4a1
NM_010444
0.94
0.65

1426606_at
Crtac1
BB426194
0.94
1.78

1434537_at
Slco3a1
BM237089
0.94
1.43

1428273_at
Abhd13
BE573480
0.93
1.53

1427071_at
Fbxo42
BC003960
0.93
1.44

1435309_at
Rbm13
AV030795
0.93
1.80

1420852_a_at
B3gnt2
AV306734
0.93
1.49

1438236_at
Nfia
BB485864
0.93
0.64

1437818_at
9430016H08Rik
AV228487
0.93
1.43

1452963_at
9530077C05Rik
AK003384
0.92
1.62

1455735_at
Ap1s3
AW259574
0.92
1.45

1427510_at
Gnai1
U38501
0.92
1.56

1417878_at
E2f1
NM_007891
0.92
0.65

1428174_x_at
Khsrp
BQ174458
0.92
0.62

1426677_at
Flna
BM233746
0.92
1.45

1428803_at
Acot6
AK014558
0.92
1.43

1418786_at
Mapk8ip2
AF220195
0.92
1.52

1434023_at
Ccdc100
BG065282
0.92
1.41

1437183_at
Lrrc4b
AW491122
0.92
1.49

1454730_at
Tapt1
AV273867
0.92
1.41

1425287_at
Zfp189
BC021326
0.92
1.40

1419224_at
Cecr6
NM_033567
0.91
1.40

1425111_at
Sorcs3
AK018111
0.91
1.69

1451833_a_at
Setdb1
BC007176
0.91
1.56

1418969_at
Skp2
AV259620
0.91
2.19

1453727_at
Esfl
BB612598
0.91
1.89

1454989_at
Cspp1
AW553463
0.91
1.68

1450679_at
5730470L24Rik
BC004090
0.91
1.50

1451033_a_at
Trpc4
BB271442
0.91
1.41

1427998_at
Lsm12
AV015526
0.91
1.46

1428911_at
Ttll4
AV231607
0.91
1.49

1443955_at
4933434E20Rik
BG074246
0.91
0.71

1427208_at
Zfp451
BC024435
0.91
1.48

1424686_at
2700008B19Rik
BF150550
0.90
1.55

1460737_at
Igbp1
AA960310
0.90
1.83

1418726_a_at
Tnnt2
NM_011619
0.90
0.67

1435214_at
Gja12
BB319817
0.90
1.54

1424755_at
Hip1
BB320674
0.90
0.68

1451734_a_at
Dbn1
AB064321
0.90
1.41

1416389_a_at
Rcbtb2
NM_134083
0.90
1.59

1426835_at
Metap1
BG064851
0.90
1.41

1421024_at
Agpat1
BB524140
0.89
0.69

1420287_at

R75193
0.89
1.41

1418839_at
Glmn
NM_133248
0.89
1.42

1421771_a_at
Ipp
NM_008389
0.89
1.51

1416348_at
Men1
AB023401
0.89
1.73

1458349_s_at

BB278163
0.89
0.64

1434815_a_at
Mapkapk3
AI451985
0.89
1.57

1453742_at
Vps33a
BG087455
0.89
1.55

1421835_at
Mtap7
NM_008635
0.89
1.49

1435841_s_at
Suclg2
BB757840
0.89
1.42

1427088_at
Ccnt2
BI872151
0.88
1.70

1428750_at
Cdc42ep2
BF453885
0.88
1.82

1431939_a_at
Mina
AK014426
0.88
1.40

1452699_at
Mett11d1
AK009576
0.88
1.65

1429249_at
4833424O15Rik
BB346520
0.88
1.71

1428757_at
Aasdhppt
AK013111
0.88
1.52

1433618_at
C330006A16Rik
BB397899
0.88
0.69

1438114_x_at
Efs
BB168690
0.88
1.50

1419400_at
Mttp
AW553649
0.87
1.42

1424842_a_at
Arhgap24
BC025502
0.87
1.43

1433820_a_at
1110012D08Rik
BB489180
0.87
1.53

1438115_a_at
Slc9a3r1
BB805362
0.87
1.65

1424894_at
Rab13
BC027214
0.87
0.66

1421235_s_at
Recql5
NM_130454
0.86
1.47

1439623_at
Otud1
BE687142
0.86
0.71

1426563_at
Zfp553
BB770954
0.86
1.47

1451136_a_at
Eif2b2
BC003326
0.86
1.42

1421895_at
Eif2s3x
NM_012010
0.86
1.83

1435076_at
2310047D13Rik
AV291303
0.85
1.53

1418145_at
Tfip11
NM_018783
0.85
1.50

1448346_at
Cfl1
NM_007687
0.85
0.71

1441867_x_at
4930534B04Rik
AI480494
0.85
0.65

1445763_at
1700013F07Rik
BG069737
0.85
0.63

1445824_at
Zfp458
AV276001
0.84
1.43

1423560_at
Nell2
AI838010
0.84
1.68

1436185_at
AI314180
BG076313
0.84
1.50

1452324_at
Pvt1
BE956863
0.84
0.70

1435005_at
Cenpe
BG916502
0.84
2.25

1435937_at
Sptlc2
BB794710
0.84
1.55

1444020_at
Ncan
BM939365
0.84
2.00

1448614_at
Nt5c2
BI202534
0.84
1.51

1444874_at
Atp5g1
BG074583
0.84
1.52

1460602_at
Dlc1
BB768194
0.84
0.61

1416521_at
Sepw1
NM_009156
0.84
0.56

1418526_at
Fusip1
NM_010178
0.83
1.48

1454745_at
Arhgap29
BG074320
0.83
0.70

1417528_at
Spag6
NM_015773
0.83
1.43

1459896_at
Pogk
AW911766
0.83
1.49

1426546_at
Tesk2
BQ179435
0.83
1.66

1431469_a_at
Cxxc5
AK015150
0.83
0.71

1449365_at
Edg8
NM_053190
0.83
1.90

1426217_at
2810441K11Rik
AF332866
0.82
1.74

1431131_s_at
A630007B06Rik
BI106826
0.82
1.41

1439844_at
8430426J06Rik
BE628126
0.82
0.70

1437122_at
Bcl2
BM119782
0.82
1.52

1456026_at
8030451K01Rik
AV303159
0.82
1.83

1418107_at
Tcea2
NM_009326
0.82
1.46

1421137_a_at
Pkib
AV047342
0.82
2.01

1419621_at
Ankrd2
NM_020033
0.82
2.02

1454727_at
Afap111
BB106834
0.82
1.69

1444395_at
Dixdc1
AW455436
0.82
1.77

1448942_at
Gng11
NM_025331
0.82
1.54

1417400_at
Rai14
NM_030690
0.81
1.74

1416714_at
Irf8
BG069095
0.81
1.82

1430135_at
Dnase2a
AK018651
0.81
0.70

1427148_at
Pja2
BF160731
0.81
1.44

1438517_at
Wwox
BB818997
0.81
1.54

1428919_at
Fgfr1op
BB667817
0.81
1.47

1460623_at
Skap2
BB753881
0.81
0.70

1417384_at
Entpd5
NM_007647
0.81
1.61

1422910_s_at
Smc6
AU022584
0.80
1.58

1460136_at
AW047481
AI462839
0.80
1.42

1423447_at
Clpx
BF020441
0.80
1.57

1441055_at
Akap2
BB431157
0.80
0.60

1434981_at
E130303B06Rik
AA116393
0.80
1.57

1431592_a_at
Sh3kbp1
AK007283
0.79
1.56

1421017_at
Nrg3
NM_008734
0.79
1.53

1460390_at
Sorl1
BI648081
0.79
1.53

1423339_s_at
Ccdc16
AI530149
0.79
1.49

1457816_at
Casp9
BB781510
0.79
0.63

1447359_at
Zfp575
AI326876
0.78
0.71

1417637_a_at
Hmg20b
NM_010440
0.78
0.61

1422472_at
Pex13
BB045429
0.78
1.45

1449214_a_at
Opa1
BC025160
0.78
1.70

1443863_at
Fndc3a
AA770704
0.78
0.64

1418417_at
Msc
NM_010827
0.78
1.60

1429162_at
1500015A07Rik
AK005240
0.78
2.31

1452884_at
Sfrs2ip
AK012092
0.78
1.64

1436020_at
D8Ertd457e
AV099356
0.77
1.59

1458249_at

BB771227
0.77
1.41

1424687_at
2700008B19Rik
BF150550
0.77
1.47

1447228_at
1110051M20Rik
BM248411
0.77
1.41

1456859_at
Lnp
BI133590
0.77
1.65

1428328_at
Nup50
AK006556
0.76
1.41

1434089_at
Synpo
BB426294
0.76
1.72

1458103_at
Ncor1
AV325208
0.76
0.69

1436326_at
Rora
BB306272
0.75
0.65

1419309_at
Pdpn
NM_010329
0.75
1.50

1439884_at
Nudt16
AI851783
0.75
0.70

1442707_at
Camk2a
BI134616
0.75
0.68

1427982_s_at
Syne2
BF582734
0.74
1.59

1429758_at
1700017B05Rik
BB046208
0.73
0.69

1435119_at

BE956710
0.73
1.59

1445421_at

AV238942
0.73
1.84

1432187_at
Nup43
AK011422
0.73
1.44

1453105_at
Zfp263
BG069835
0.72
1.48

1459472_at
AU022583
AU022583
0.72
1.83

1457426_at
1700048O20Rik
BE980678
0.72
0.69

1422557_s_at
Mt1
NM_013602
0.71
0.88

1423732_at
Tram1
BC012401
0.71
0.90

1428281_at
Trub1
AK011362
0.71
0.97

1440795_x_at
Rabep2
BB259371
0.71
1.12

1417886_at
Ints5
BE307471
0.71
0.91

1429232_at
2610528B01Rik
AK012160
0.71
1.09

1436393_a_at
Trim37
BG065227
0.71
1.03

1438278_a_at
BC003993
BB532946
0.71
1.07

1440161_at
Mmp16
BB378819
0.71
1.37

1442408_at
2010305B15Rik
BE981170
0.71
1.05

1453783_at
Pura
BE864772
0.71
0.93

1421375_a_at
S100a6
NM_011313
0.71
0.96

1424942_a_at
Myc
BC006728
0.71
0.88

1426265_x_at
Dlat
AV336908
0.71
0.94

1426583_at
Atf2
BM119623
0.71
1.15

1427104_at
Zfp612
BG076162
0.71
0.99

1429790_at
Brunol6
AK018246
0.71
0.93

1438684_at
Nuak1
BF021001
0.71
1.57

1452127_a_at
Ptpn13
BM236743
0.71
1.01

1420767_at
1700019G17Rik
NM_029331
0.71
0.88

1433628_at
Coq10a
BQ044689
0.71
1.03

1440512_at
AW048066
BF471100
0.71
0.91

1444139_at
Ddit41
BG797099
0.71
0.80

1446244_at
Zyg11b
BB077439
0.71
0.94

1452609_at
Cox4nb
BI133691
0.71
0.80

1423757_x_at
Igfbp4
BC019836
0.71
0.83

1424430_at
Mterfd2
BC002280
0.71
1.22

1426289_at
Qrich1
BC006738
0.71
0.93

1434301_at
D330050I23Rik
BE303700
0.71
0.82

1460362_at
2410001C21Rik
BC004803
0.71
0.98

AFFX-BioC-

AFFX-
0.71
0.86

5_at

BIOC-5

1419101_at
Sin3a
NM_011378
0.71
1.39

1419606_a_at
Tnnt1
NM_011618
0.71
0.96

1429601_x_at
1110019K23Rik
AK003824
0.71
1.37

1433500_at
Dennd2a
AV260555
0.71
1.17

1458163_at
BC066028
AV328953
0.71
0.93

1437623_x_at
Xrcc3
BB251723
0.71
0.77

1449307_at
Dbndd1
NM_028146
0.71
0.88

1450448_at
Stc1
BQ032752
0.71
1.02

1459804_at
Crebbp
BB475090
0.71
1.27

1435077_at
Asxl1
BE956516
0.71
0.87

1445461_at

BB096245
0.71
1.08

1448495_at
Tsta3
NM_031201
0.71
1.01

1448923_at
Prkra
NM_011871
0.71
0.79

1456061_at
Gimap8
BM243674
0.71
0.91

1457262_at
2610207I05Rik
BB024162
0.71
1.32

1417204_at
Kdelr2
AJ278133
0.71
1.12

1419789_at
Centg1
AI605509
0.71
2.20

1428903_at
3110037I16Rik
AK014134
0.71
0.95

1449935_a_at
Dnaja3
AK004575
0.71
1.13

1433639_at
5730593F17Rik
AW548096
0.70
0.72

1436388_a_at
3830406C13Rik
BB763097
0.70
0.93

1436581_at
Ccdc39
BQ174391
0.70
1.18

1436875_at
Dnm3
BB130476
0.70
1.12

1453343_s_at
Vrk2
AK012664
0.70
1.08

1424228_at
Polr3h
AK019868
0.70
0.93

1426138_a_at
Ube2j2
AF296656
0.70
1.13

1432972_at
4921518B13Rik
AK014916
0.70
1.51

1436833_x_at
Ttl11
BB251824
0.70
0.94

1452721_a_at
Ccdc53
BM210204
0.70
0.96

1418236_s_at
Atg5
AV168389
0.70
1.15

1427098_at
Wwp1
BI102531
0.70
0.85

1429243_at
1110054O05Rik
BG076073
0.70
0.95

1431059_x_at
Htatsf1
AK012522
0.70
1.01

1436099_at
AI836003
AV294537
0.70
0.90

1437591_a_at
Wdr1
AW542545
0.70
0.99

1452880_at
Znhit3
AK003721
0.70
0.96

1425580_a_at
Pik3c3
BC024675
0.70
1.24

1426624_a_at
Ypel3
BI660196
0.70
0.89

1429710_at
Styx
BB812465
0.70
1.24

1436456_at
9130023D20Rik
BQ031896
0.70
1.34

1438028_at
4930535B03Rik
BB817800
0.70
1.13

1460609_at
Ints8
BB224305
0.70
0.78

1426078_a_at
Gpr108
AF376726
0.70
1.02

1440895_at
LOC665193
BB122340
0.70
0.98

AFFX-r2-Ec-

“AFFX-R2-

bioC-3_at

EC-BIOC-3”
0.70
0.96

1418970_a_at
Bcl10
AF100339
0.70
1.02

1419257_at
Tcea1
BC006022
0.70
0.87

1422857_at
Trip4
AV350958
0.70
1.02

1423191_at
Fnbp4
AK013152
0.70
1.18

1435864_a_at
1810063B05Rik
BG975168
0.70
0.89

1438269_at
Zbtb38
BB278987
0.70
0.73

1456366_at
1700015F17Rik
BB833901
0.70
0.82

1460670_at
Riok3
NM_024182
0.70
1.00

1425203_at
Ddx19b
BE553267
0.70
0.79

1428172_at
Prpf39
BB460975
0.70
1.21

1431429_a_at
Arl4a
AK006286
0.70
1.11

1436617_at
Cetn4
AV211098
0.70
0.92

1437449_at
Rsad1
BB818348
0.70
0.95

1456884_at
LOC67660
AI854003
0.70
0.95

1419655_at
Tle3
NM_009389
0.70
1.40

1430694_at
Star
BE630656
0.70
0.94

1435744_at
6720401G13Rik
BG075556
0.70
0.94

1437202_at
Mysm1
BB494594
0.70
1.00

1449746_s_at
Krr1
AU020154
0.70
0.90

1423144_at
Pik3ca
AI528567
0.70
1.13

1426879_at
1190005F20Rik
AK004493
0.70
1.17

1428285_at
8430427H17Rik
AK018446
0.70
1.05

1443870_at
Abcc4
BB291885
0.70
1.17

1452445_at
Slc41a2
BC026874
0.70
1.02

1460434_at
Fundc2
AI510221
0.70
0.88

1423363_at
Sort1
AV247637
0.69
0.80

1436373_at
Map3k10
AA789425
0.69
0.97

1437606_at

BB829614
0.69
0.89

1448222_x_at
Cox8a
NM_007750
0.69
0.97

1449999_a_at
Cacna2d1
NM_009784
0.69
1.01

1460639_a_at
Atox1
NM_009720
0.69
0.96

1419046_at
Brp16
NM_021555
0.69
1.25

1421204_a_at
Nudt16
NM_029385
0.69
0.94

1422622_at
Nos3
NM_008713
0.69
1.16

1439332_at
Ddit41
AV251625
0.69
0.92

1455661_at

BB211563
0.69
0.94

1429229_s_at
4930534B04Rik
BE980134
0.69
0.77

1450849_at
Hnrpu
BF228203
0.69
2.02

1430999_a_at
Scoc
BM932452
0.69
0.96

1433969_at
AU067824
AU067824
0.69
1.01

1460005_at
AF013969
BB542960
0.69
0.95

1435027_at
Golga1
AW552851
0.69
1.09

1439341_at
AK220484
BB203252
0.69
1.12

1450881_s_at
Gpr137b
AK010724
0.69
1.02

1417065_at
Egr1
NM_007913
0.69
0.99

1424922_a_at
Brd4
BC008532
0.69
0.99

1427390_at
Bloc1s3
BC025913
0.69
0.94

1450083_at
Cnot4
AI448404
0.69
0.97

1458464_at
Hecw2
BB445169
0.69
1.22

1453381_at
C030033M12Rik
BB771017
0.69
1.04

1427992_a_at
Rab12
BF729629
0.69
1.01

1419130_at
Deadc1
NM_025748
0.69
1.94

1428600_at
Nin
AK014241
0.69
0.99

1431820_at
4632404H12Rik
AK014549
0.69
0.83

1447827_x_at
Spop
AV353779
0.69
1.15

1420579_s_at
Cftr
NM_021050
0.68
2.52

1429058_at
1110004B13Rik
AK003403
0.68
0.84

1435237_at
2310009A05Rik
AV004950
0.68
0.81

1453684_s_at
Zc3h15
AK005661
0.68
1.03

1454968_at
1110034A24Rik
AV011961
0.68
1.00

1418135_at
Aff1
NM_133919
0.68
1.10

1418971_x_at
Bcl10
AF100339
0.68
0.99

1437003_at

BB323930
0.68
0.71

1448306_at
Nfkbia
NM_010907
0.68
1.13

1416607_at
4931406C07Rik
AI461712
0.68
1.13

1431120_a_at
Golga1
BC024637
0.68
0.97

1435544_at
Exosc6
BB446614
0.68
1.10

1439888_at

BB271581
0.68
1.17

1444150_at
Epb4.1
BB462549
0.68
0.88

1448218_s_at
Ywhaz
AV027921
0.68
0.86

1457825_x_at
Tcn2
BB515151
0.68
1.25

AFFX-r2-Ec-

“AFFX-R2-

bioC-5_at

EC-BIOC-5”
0.68
0.89

1418432_at
Cab39
AK005226
0.68
1.39

1426230_at
Sphk2
AK016616
0.68
0.81

1433743_at
Dach1
BG075820
0.68
0.82

1437203_at
Cbll1
BB460904
0.68
1.17

1458951_at
Vrk1
AV341598
0.68
1.07

1421087_at
Per3
NM_011067
0.68
0.95

1455074_at
Efcab1
AV307860
0.68
1.31

AFFX-r2-Ec-

“AFFX-R2-

bioB-3_at

EC-BIOB-3”
0.68
0.86

1420053_at
Psmb1
C81484
0.68
0.93

1421097_at
Endog
NM_007931
0.68
0.97

1426326_at
Zfp91
U05343
0.68
0.99

1434423_at
Gulp1
BB138485
0.68
0.83

1437866_at
Dusp18
AV276425
0.68
1.26

1418024_at
Narg1
BG067031
0.68
1.14

1430168_at
Cstad
AK020589
0.68
0.99

1433720_s_at
Ndg2
AI647775
0.68
0.95

1440060_at
EG331480
AV306340
0.68
1.03

1453754_at
S100pbp
AK015229
0.68
0.76

1457232_at
Fbxl21
BE946365
0.68
0.93

1436104_a_at
2310015A05Rik
BG068839
0.68
0.93

1439780_at
Rpl711
BG071958
0.67
0.56

1451270_at
Dusp18
BC020036
0.67
1.11

1453993_a_at
Bnip2
AK014659
0.67
0.88

1428635_at
Comtd1
AK007659
0.67
0.97

1433793_s_at
Nrip2
AW491344
0.67
0.97

1439497_at
4933415E08Rik
BG065013
0.67
0.68

1455463_at
Phyhip
BB427286
0.67
0.76

1419193_a_at
Gmfg
NM_022024
0.67
1.00

1447641_at
Dmwd
AV354897
0.67
0.91

1457354_at
Krt222
AI845957
0.67
0.83

1458425_at
My16b
BM945176
0.67
1.04

1420530_at
Neud4
AW553317
0.67
0.80

1423456_at
Bzw2
BM932775
0.67
1.04

1423477_at
Zic1
BB361162
0.67
1.10

1429914_at
Epc1
AK017858
0.67
1.03

1439699_at
Pgr
BB525237
0.67
0.84

1443579_s_at
Depdc6
AI957118
0.67
0.96

1430222_at
9130007G19Rik
BB538672
0.67
0.86

1454086_a_at
Lmo2
AK013416
0.67
1.01

1428659_at
Phf7
AK005852
0.67
0.67

1440202_at
5930412I24Rik
BB036159
0.67
1.60

1440338_at
E430028B21Rik
BM933532
0.67
0.98

1417387_at
Med31
NM_026068
0.67
0.98

1423630_at
Cygb
BM899392
0.67
0.92

1424486_a_at
Txnrd1
BB284199
0.67
0.84

1434145_s_at
Serhl
C80355
0.67
1.12

1435365_at
4732415M23Rik
BB614874
0.67
1.07

1429842_at
Mdh1b
AK007262
0.67
1.04

1436796_at
1110061A14Rik
BB237072
0.67
1.15

1451751_at
Ddit41
AF335325
0.67
0.95

1452849_at
Sin3b
AK013202
0.67
0.87

1418408_at
Zfand1
NM_025512
0.67
1.04

1430286_s_at
4930402H24Rik
AK013448
0.67
0.93

1437840_s_at
LOC673662
BB222646
0.67
1.05

1444001_at
Strbp
AW488249
0.67
1.51

1457669_x_at
Rfc2
AV096765
0.67
0.96

1432378_at
C030004G16Rik
AK021043
0.66
1.03

1451132_at
Pbxip1
AV220340
0.66
1.01

1417107_at
Tpd5212
NM_025482
0.66
0.94

1421997_s_at
Itga3
NM_013565
0.66
1.11

1448851_a_at
Dnajc5
AK012029
0.66
1.11

1451213_at
Pex11b
BB010355
0.66
1.07

1456645_at
Wdr25
BB352668
0.66
0.72

1422842_at
Xrn2
NM_011917
0.66
1.04

1452517_at
Plekhh1
BC006045
0.66
1.25

1424169_at
Tax1bp3
BC008166
0.66
0.73

1424207_at
Smarca5
BI661719
0.66
1.36

1428841_at
Best1
AK006549
0.66
0.99

1425528_at
Prrx1
L06502
0.66
1.16

1451020_at
Gsk3b
BB831420
0.66
0.90

1452228_at
Tbc1d23
AV209678
0.66
0.94

1419144_at
Cd163
NM_053094
0.66
0.96

1441742_at
Kcnh5
AW048994
0.66
1.08

1448553_at
Myh7
NM_080728
0.66
0.86

1416622_at
Wbscr16
NM_033572
0.66
1.37

1423834_s_at
Gga1
BC026802
0.66
0.92

1449470_at
Dlx1
NM_010053
0.66
0.87

1426360_at
Zc3h11a
AV328883
0.66
1.33

1419266_at
Nfyb
AV250496
0.65
1.22

1432391_at
Ccdc21
AK010621
0.65
0.84

1441453_at
Dennd2a
BB341122
0.65
0.94

1455801_x_at
Tbcd
BB392080
0.65
1.03

1429125_at
Zbtb9
AV283825
0.65
0.93

1426584_a_at
Sord
BI143942
0.65
0.85

AFFX-BioDn-

“AFFX-

3_at

BIODN-3”
0.65
0.90

1416112_at
Cox8a
NM_007750
0.65
0.96

1418172_at
Hebp1
AF117613
0.65
1.21

1427334_s_at
2810474O19Rik
BE196832
0.65
0.95

1429362_a_at
Sf3b2
BF022081
0.65
1.01

1453006_at
Fgfbp3
AI506568
0.65
1.00

1424899_at
Nmnat3
BC005737
0.65
0.95

1437018_at
Pnma2
BG072348
0.65
1.16

1422164_at
Pou3f4
X66603
0.65
0.99

1452817_at
Smyd3
AK010447
0.65
0.99

1437118_at
Usp7
C77542
0.65
1.76

1460021_at
EG626231
BB825323
0.65
1.28

1422662_at
Lgals8
AI987967
0.64
0.94

1428198_at
Adal
AK016299
0.64
1.10

1423184_at
Itsn2
AI326108
0.64
0.76

1426631_at
Pus7
BM199125
0.64
1.11

1437694_at
Zfp809
AW536892
0.64
0.92

1455586_at
Rnf168
BB275228
0.64
0.49

1447494_at
D7Bwg0826e
R74675
0.64
1.01

1430242_at
Rwdd1
AV356665
0.64
1.14

1430745_at
5930409G06Rik
AK020028
0.64
0.71

1458685_at
Garnl1
BB552367
0.64
1.03

1438477_a_at
Mcee
AA960125
0.64
0.86

1446357_at
BC020402
BB029175
0.64
1.47

1447097_at
8430426H19Rik
BG068083
0.64
0.88

1438491_x_at
A530054K11Rik
BF227108
0.63
1.22

1448547_at
Rassf3
BB703307
0.63
0.85

1450543_at
Kctd10
NM_026145
0.63
0.93

1423284_at
Mansc1
AK002644
0.63
0.99

1441316_at
LOC677060
BB234335
0.63
0.59

1452594_at
Dusp11
BB004080
0.63
1.19

1428943_at
Nudt13
BE134119
0.63
1.05

1438732_at
6430511F03
BE947483
0.63
1.04

1453771_at
Gulp1
BF144687
0.63
0.92

1417219_s_at
Tmsb10
NM_025284
0.63
0.67

1424487_x_at
Txnrd1
BB284199
0.63
1.09

1426458_at
Slmap
BB473571
0.63
0.92

1448417_at
Ninj1
NM_013610
0.63
0.90

1452161_at
Tiparp
BB707122
0.63
0.67

1427030_at
Ccdc52
BG067674
0.63
0.83

1442281_at
D4Ertd796e
BG069783
0.63
0.86

1451677_at
Narf
BI452475
0.63
1.02

1429040_at
6820431F20Rik
BE692399
0.63
1.33

1416661_at
Eif3s10
AW701127
0.63
1.18

1421479_at
Zfp318
NM_021346
0.62
0.78

1452187_at
Rbm5
BB541007
0.62
1.32

1456120_at
3110001I20Rik
BB427489
0.62
0.95

1428863_at
Ankrd39
AK018681
0.62
1.00

1457038_at
Frem2
BM201912
0.62
1.71

1457072_at
Bcl11a
BF731393
0.62
1.04

1419924_at
Fnip1
AW557298
0.62
1.60

1425927_a_at
Atf5
AF375476
0.62
1.00

1426832_at
Ddx26b
BB460424
0.62
1.08

1454777_at
Slco2b1
BB553107
0.62
0.92

1418323_at
Fem1b
BM232562
0.61
1.13

1425309_at
Catsper2
AF411816
0.61
0.98

1456517_at
Tmem44
BB035891
0.61
0.92

1429260_at
1810014B01Rik
AW985991
0.61
1.07

1445099_at

BB383493
0.61
1.01

1452474_a_at
Art3
AJ311773
0.61
1.07

1439073_at
Mllt4
BB821719
0.61
1.20

1438481_at
AW047464
AI854375
0.61
0.88

1442024_at
Ppp1r3e
BB328892
0.61
0.98

1453591_at
5730437N04Rik
AK017626
0.61
1.06

1418894_s_at
Pbx2
NM_017463
0.61
0.97

1440756_at

BB712642
0.61
0.82

1456419_at
5730455P16Rik
BI108098
0.61
0.87

1437305_at
Zfp770
AV296864
0.60
0.98

1438928_x_at
Ninj1
BB252065
0.60
0.82

1424117_at
BC056474
BC024937
0.60
0.99

1454830_at
Fbn2
AV010392
0.60
1.05

1458353_at
Nwd1
BB173301
0.60
0.93

1427456_at
Wdfy3
BF150771
0.60
1.09

1426421_s_at
Rbm26
AK005802
0.60
1.39

1454174_a_at
C330007P06Rik
AK021190
0.60
2.16

1423353_at
Crispld1
BE693025
0.60
0.90

1437108_at
Lsm6
BE949068
0.60
0.88

1437884_at
Arl5b
BB545273
0.60
0.85

1447344_at

AI447264
0.60
1.04

1450759_at
Bmp6
NM_007556
0.60
1.22

1422230_s_at
Cyp2a5
NM_007812
0.60
0.81

1428047_s_at
Zfx
BC028506
0.60
1.07

1432269_a_at
Sh3kbp1
AK018032
0.59
1.49

1440215_at
“OTTMUSG

00000004461”
BG068916
0.59
0.93

1453083_at
6430701C03Rik
AK018294
0.59
0.99

1455998_at
Zc3h11a
BM232738
0.59
0.96

1427924_at
Stx17
BE943517
0.59
0.72

1449357_at
2310030G06Rik
NM_025865
0.59
0.82

1438032_at
Lrch1
BB755336
0.59
0.74

1417938_at
Rad51ap1
BC003738
0.59
1.02

1453320_at
1700027A23Rik
AK006407
0.59
1.02

1449109_at
Socs2
NM_007706
0.58
1.03

1449677_s_at
Tmem38b
C77858
0.58
1.11

1452748_at
Xrcc3
AW537713
0.58
1.06

1457693_a_at
6430537H07Rik
AV343405
0.58
0.85

1441306_at
6820408C15Rik
AW048149
0.57
0.97

1439783_at
C330018D20Rik
BB809870
0.57
0.79

1428826_at
Nr6a1
AK007201
0.57
0.94

1456067_at
Gli3
AW546010
0.57
0.87

1430520_at
Cpne8
AW548480
0.57
1.29

1441161_at
B230216G23Rik
BB307791
0.57
2.63

1430240_a_at
Clgn
AK019534
0.56
1.04

1439555_at
Rlf
BB704706
0.56
1.30

1426534_a_at
Arfgap3
BG067878
0.56
0.78

1445564_at

BE688513
0.56
1.32

1456674_at
E130016E03Rik
BB772877
0.55
1.05

1435351_at
2310026E23Rik
AA189197
0.55
1.25

1429984_at
5730455O13Rik
AK017666
0.55
1.14

1447476_at
Abcc10
BB079952
0.55
0.56

1419390_at
Pde10a
BQ180352
0.55
0.87

1442927_at
Ptk2b
BB548690
0.55
1.40

1418707_at
Bag4
NM_026121
0.55
1.58

1457625_s_at
Cdkl2
BE979937
0.55
1.12

1456333_a_at
Arhgap6
BB367529
0.54
0.93

1442588_at
EG329217
BB117375
0.53
0.80

1458438_at
Ccdc122
AW554226
0.53
0.94

1441106_at
Zfand5
BB320444
0.52
1.11

1447809_x_at

BB080932
0.52
1.41

1452473_at
Prr15
AJ132433
0.51
0.58

1453309_at
9330179D12Rik
BB749938
0.51
0.70

1426478_at
Rasa1
AA124924
0.51
1.36

1449669_at
Rabif
AW549708
0.51
0.91

1458397_at
Dnaja1
BB260315
0.50
0.93

1449141_at
Fblim1
BG070068
0.49
0.80

1444141_at
Snx13
BB828008
0.49
1.95

1420533_at
Gucy1a3
AK004815
0.49
0.92

1445165_at
1200003I07Rik
BM194850
0.48
0.91

1460285_at
Itga9
NM_133721
0.46
1.44

1426612_at
Tipin
AK011357
0.45
0.84

1441871_at
1810044D09Rik
AV057045
0.45
1.12

1419230_at
A830036E02Rik
NM_010661
0.43
1.03

1441123_at
ORF34
BB023801
0.43
1.41

1449388_at
Thbs4
NM_011582
0.41
2.16



Probe = Affymetrix probe identification;

Symbol = official gene symbol;

FCH D1 = fold change in striatonigral (D1) cells;

FCH D2 = fold change in striatopallidal (D2) cells.




















































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































TABLE 16



Gene translation changes upon chronic cocaine treatment












Probe
Symbol
GenBank
FCHD1
FCHD2
Probe
Symbol
GenBank
FCHD1
FCHD2














1430648_at
Scn2b
AK013326
12.83
0.60
1446981_at
A830010M20Rik
BB127813
2.30
0.99

1455799_at
Rorb
BB751387
3.11
1.93
1435635_at
Pcmtd1
BB549335
2.29
0.63

1445081_at
A930041I02Rik
BB335888
3.02
1.23
1424708_at
Tmed10
BI409239
2.27
1.20

1452906_at
Gtl2
BE990468
2.92
0.51
1448944_at
Nrp1
AK011144
2.26
1.91

1448606_at
Edg2
U70622
2.86
1.04
1434393_at
Usp34
BM235696
2.23
1.35

1442061_at
Btbd7
BB199960
2.60
1.60
1421090_at
Epb4.1l1
NM_013510
2.21
1.10

1421978_at
Gad2
AF326550
2.56
0.76
1460330_at
Anxa3
AW702161
2.19
1.43

1426458_at
Slmap
BB473571
2.50
0.79
1429256_at
Gtl2
AU067739
2.17
0.88

1432558_a_at
Mal
AK019046
2.50
0.82
1427464_s_at
Hspa5
AJ002387
2.16
1.44

1433599_at
Baz1a
BM239162
2.48
2.03
1436898_at
Sfpq
B1738328
2.14
1.33

1417029_a_at
Trim2
BB283676
2.45
1.80
1455607_at
Rspo3
BG072958
2.12
0.97

1438388_at
Nxph1
BB078029
2.45
0.77
1428306_at
Ddit4
AK017926
2.10
0.26

1418189_sat
Ramp2
AF146523
2.42
0.99
1440282_at
Tulp4
BB268998
2.10
1.78

1429769_at
Pggt1b
BI107300
2.39
0.83
1454950_at
B930006L02Rik
BB699417
2.10
0.94

1457077_at

BB306048
2.37
1.49
1417602_at
Per2
AF035830
2.07
2.79

1438702_at
Flrt2
BG075699
2.35
1.05
1421970_a_at
Gria2
NM_013540
2.07
0.79

1418188_a_at
Ramp2
AF146523
2.34
1.16
1454655_at
Dgkd
BB094429
2.07
1.58

1425693_at
Braf
M64429
2.32
1.10
1452507_at
Dlx6
AF022078
2.04
0.57

1418937_at
Dio2
AF177196
2.30
0.88
1419029_at
Ero1l
BM234652
2.04
1.07

1455182_at
Kif1b
AV104668
2.02
1.10
1437002_at
C030011O14Rik
BB303443
1.79
1.18

1450760_a_at
Ing3
BB020556
2.01
0.61
1421189_at
Gabrb3
BB367779
1.79
0.99

1417604_at
Camk1
NM_133926
2.00
1.33
1420502_at
Sat1
NM_009121
1.78
1.07

1418350_at
Hbegf
L07264
1.99
1.32
1433582_at
1190002N15Rik
AV309085
1.78
1.29

1449372_at
Dnajc3
BE624323
1.99
0.88
1423363_at
Sort1
AV247637
1.78
1.17

1446147_at

BB436856
1.98
0.69
1417875_at
Ddx50
NM_053183
1.78
0.93

1429095_at
Cenpp
AK006234
1.97
0.84
1434673_at
Gpr22
BB429977
1.78
0.53

1428157_at
Gng2
AV021455
1.97
1.02
1424028_at
5830457O10Rik
BC023107
1.78
0.49

1455831_at
Fus
BE985138
1.97
0.70
1421116_a_at
Rtn4
NM_024226
1.77
0.78

1424186_at
Ccdc80
BG074158
1.95
1.24
1428484_at
Osbpl3
AK004768
1.77
1.73

1428112_at
Armet
AK014338
1.95
1.53
1426951_at
Crim1
AK018666
1.77
1.15

1417110_at
Man1a
BB070019
1.95
1.64
1437857_at
Dpy19l3
AV367203
1.77
1.51

1448545_at
Sdc2
AU021035
1.94
0.98
1420850_at
Crnkl1
AV143435
1.76
0.80

1452089_at
Cacnb4
AW106929
1.94
0.88
1423066_at
Dnmt3a
BB795491
1.76
1.38

1450708_at
Scg2
NM_009129
1.94
1.24
1448008_at
Ankhd1
BG081523
1.76
1.31

1426340_at
Slc1a3
BB357585
1.94
1.42
1421961_a_at
Dnajb5
AI664344
1.76
1.31

1448115_at
Htf9c
NM_008307
1.93
0.97
1460705_at
Rps6kb1
AI451506
1.76
0.85

1424486_a_at
Txnrd1
BB284199
1.92
1.10
1428361_x_at
Hba-a1
AK011116
1.76
1.22

1442695_at
C030007I01Rik
AW494399
1.91
1.41
1423720_a_at
Sar1a
BC005549
1.75
0.96

1418322_at
Crem
AI467599
1.91
1.84
1425130_a_at
Ptpn5
U28216
1.75
0.87

1453796_a_at
Ergic2
AK016275
1.90
0.69
1450208_a_at
Elmo1
NM_080288
1.74
1.34

1437122_at
Bcl2
BM119782
1.90
1.66
1438578_a_at
Btbd10
BG064119
1.74
1.17

1420886_a_at
Xbp1
NM_013842
1.90
1.27
1436661_at
Dpp10
BQ174532
1.74
1.28

1420011_s_at
Xbp1
C77390
1.89
1.19
1435471_at
Zfp708
BE994129
1.74
0.57

1421354_at
Prkg2
NM_008926
1.89
0.59
1453238_s_at
A130040M12Rik
AU018141
1.74
1.68

1443846_x_at
Pank2
BB402666
1.88
0.39
1428946_at
Ube1l2
BB417360
1.74
0.76

1428600_at
Nin
AK014241
1.88
1.04
1452377_at
Mll1
AK017541
1.73
1.16

1422134_at
Fosb
NM_008036
1.88
1.16
1439434_x_at
Pcmt1
BB317673
1.73
1.04

1449870_a_at
Atp6v0a2
NM_011596
1.88
1.08
1438796_at
Nr4a3
BE692107
1.73
1.85

1420720_at
Nptx2
NM_016789
1.86
1.73
1426326_at
Zfp91
U05343
1.73
1.14

1449552_at
Zfr
NM_011767
1.86
0.60
1419228_at
Elac1
NM_028983
1.72
1.48

1452754_at
Creld2
AK017880
1.86
1.41
1419647_a_at
Ier3
NM_133662
1.72
1.08

1443579_s_at
Depdc6
AI957118
1.84
0.61
1442887_at
Gpr39
BQ175060
1.71
1.05

1434674_at
Lyst
BB463428
1.84
1.23
1456137_at
Nrxn3
BB132137
1.71
0.68

1439780_at
Rpl7l1
BG071958
1.84
0.81
1428834_at
Dusp4
AK012530
1.71
1.84

1418380_at
Terf1
NM_009352
1.84
0.92
1435520_at
Msi2
BQ174527
1.71
1.58

1448943_at
Nrp1
AK011144
1.83
1.16
1449164_at
Cd68
BC021637
1.71
1.69

1455403_at
Manea
BB035197
1.83
0.97
1459750_s_at
Gpr123
AU015577
1.71
1.25

1440371_at

BQ176223
1.82
1.07
1436343_at
Chd4
BM502696
1.70
1.37

1436387_at
Homer1
BB398124
1.82
1.91
1420048_at
C78859
C78859
1.70
0.94

1438458_a_at
Sfpq
BF224766
1.82
1.21
1456769_at
Dusp3
BQ266434
1.70
1.37

1428130_at
Lman1
BG071597
1.82
1.42
1451311_a_at
Adipor1
BC014875
1.70
0.97

1449289_a_at
B2m
BF715219
1.82
1.50
1455085_at
1700086L19Rik
BI526033
1.69
1.19

1433595_at
Slc35d1
BB409668
1.82
1.34
1430869_a_at
Habp4
BF460630
1.69
0.96

1417111_at
Man1a
BB070019
1.82
1.31
1427256_at
Vcan
BM251152
1.69
1.32

1437433_at
B3galt2
BB254922
1.82
0.97
1437667_a_at
Bach2
AW553304
1.68
2.26

1434913_at
Hmgcll1
BF471959
1.81
0.72
1454920_at
Uhrf2
BQ266387
1.68
1.28

1435770_at
Txndc13
BB215068
1.81
1.03
1426736_at
Gspt1
AB003502
1.68
1.10

1425942_a_at
Gpm6b
AF254879
1.81
0.99
1415986_at
Clcn4-2
NM_011334
1.68
1.04

1416447_at
Tmem30a
BE986812
1.80
0.97
1437339_s_at
Pcsk5
BB241731
1.68
1.48

1454158_at
Mpp7
AK012883
1.80
1.19
1428967_at
Igf1r
BB446952
1.67
1.38

1416064_a_at
Hspa5
NM_022310
1.80
1.54
1457142_at
Efcbp1
BB667731
1.67
1.20

1417812_a_at
Lamb3
NM_008484
1.80
0.95
1422886_a_at
Clk4
NM_007714
1.67
0.66

1450029_s_at
Itga9
NM_133721
1.79
1.14
1422579_at
Hspe1
NM_008303
1.66
0.98

1429718_at
Slitrk5
BB782729
1.79
1.11
1439333_at
Kcnv1
BB078927
1.66
0.80

1448701_a_at
Krit1
AF310134
1.66
1.12
1436051_at
Myo5a
BQ174518
1.58
0.95

1421900_at
Eif2ak1
BB077436
1.66
0.85
1434421_at
Islr2
BB344549
1.58
0.51

1452237_at
Hrb
BB130716
1.65
1.18
1430667_at
Pcdh10
BB077413
1.58
1.16

1429159_at
Itih5
AK018605
1.65
0.95
1435158_at
Rbm12b
BB796313
1.58
1.80

1416714_at
Irf8
BG069095
1.64
1.32
1418104_at
Nrip3
NM_020610
1.58
0.58

1428142_at
Etv5
AK003461
1.64
1.63
1416093_a_at
Mrpl20
NM_025570
1.58
1.17

1436094_at
Vgf
BF458396
1.64
1.57
1426187_a_at
Hax1
AF465243
1.58
1.16

1452168_x_at
Gspt1
AB003502
1.64
1.29
1439605_at

BB464198
1.58
0.69

1456964_at
Rbm12
BB104271
1.63
0.76
1425660_at
Btbd3
BC018174
1.57
2.19

1424686_at
2700008B19Rik
BF150550
1.63
0.96
1423294_at
Mest
AW555393
1.57
1.22

1449342_at
Ptplb
NM_023587
1.63
0.94
1416085_s_at
Zfand5
AA124553
1.57
1.11

1423314_s_at
Pde7a
BG070255
1.63
1.47
1421280_at
Gabra1
Z36357
1.57
0.66

1430520_at
Cpne8
AW548480
1.63
0.47
1424405_at
Mbip
BC002277
1.57
0.90

1439293_at
BC031353
BB369212
1.63
0.87
1453020_at
1810048J11Rik
AK007829
1.57
1.05

1426934_at
Nhsl1
BC013565
1.63
1.44
1428920_at
Hmg20a
AI987819
1.57
1.13

1429249_at
4833424O15Rik
BB346520
1.63
1.19
1450247_a_at
Scamp5
NM_020270
1.57
0.89

1436645_a_at
Cnot4
BB066603
1.62
1.04
1456796_at
Snai3
BB238756
1.57
1.13

1459858_x_at
Crtac1
AV354465
1.62
1.11
1438682_at
Pik3r1
BB426164
1.56
1.00

1437114_at
A930001N09Rik
AV230892
1.62
1.10
1439900_at
Thtpa
BB308405
1.56
1.55

1441197_at
9530059O14Rik
AW494454
1.62
1.47
1423648_at
Pdia6
BC006865
1.55
1.07

1442212_at
Cpd
BB519218
1.62
1.36
1434246_at
L3mbtl3
BB022070
1.55
1.08

1449799_s_at
Pkp2
AA516617
1.62
1.53
1427358_a_at
Dapk1
BC026671
1.55
0.80

1420947_at
Atrx
BB825830
1.62
0.51
1435357_at
D4Wsu53e
BE652553
1.55
1.13

1434228_at
Ppm2c
AV255921
1.62
1.09
1460440_at
Lphn3
BE945410
1.55
1.04

1427470_s_at
Napb
AU067119
1.62
0.88
1424342_at
Fyttd1
BC010204
1.55
1.08

1430511_at
1500037O19Rik
AK005367
1.62
1.15
1425608_at
Dusp3
BC016269
1.55
1.18

1443017_at
Cpeb2
BB235708
1.61
1.11
1426824_at
Psme4
BM195254
1.55
1.10

1417404_at
Elovl6
NM_130450
1.61
1.30
1424532_at
Ylpm1
AB033168
1.55
1.14

1419211_s_at
4933424B01Rik
NM_138757
1.61
1.03
1426490_at
Bfar
AK013874
1.55
0.69

1423162_s_at
Spred1
BQ044290
1.61
1.39
1424350_s_at
Lpgat1
BE987427
1.55
1.20

1448669_at
Dkk3
AK004853
1.61
1.42
1457277_at
BC038925
AI314927
1.55
1.14

1428999_at
Ph13
AK010013
1.61
0.79
1435641_at
9530018I07Rik
BB109391
1.55
1.27

1440891_at
Gria4
AV336506
1.61
1.49
1422553_at
Pten
AA214868
1.55
1.10

1434883_at
Mtdh
AV083741
1.61
0.82
1455009_at
Cpd
AW550842
1.55
1.19

1421815_at
Epdr1
AF353717
1.60
1.63
1433581_at
1190002N15Rik
AV309085
1.54
1.21

1417416_at
Kcna1
NM_010595
1.60
1.12
1419256_at
Spnb2
BM213516
1.54
1.22

1433887_at
Dnajc3
AV024539
1.60
1.24
1428793_at
Slc36a1
AK017918
1.54
1.20

1420580_at
4930429B21Rik
NM_026249
1.60
0.91
1418772_at
BC016423
NM_134063
1.54
0.75

1435250_at
Ints8
BB206299
1.60
1.39
1423247_at
Txndc4
BI100077
1.54
0.95

1421768_a_at
Homer1
NM_011982
1.60
0.96
1434283_at
Arid5b
BB079486
1.54
1.60

1417977_at
Eif4e3
BCO27014
1.60
0.83
1424089_a_at
Tcf4
U16321
1.53
1.13

1419988_at
Map3k7
C87327
1.60
1.12
1426023_a_at
Rabep1
AF248489
1.53
1.04

1421346_a_at
Slc6a6
NM_009320
1.60
1.04
1441603_at
Sstr3
BQ174132
1.53
1.86

1428344_at
Ppapdc2
BB229589
1.60
1.25
1443969_at
Irs2
BE199054
1.53
1.20

1439661_at
Slc16a14
BB426676
1.59
0.86
1450011_at
Hsd17b12
AK012103
1.53
1.03

1419110_at
Riok1
BM228482
1.59
0.78
1454816_at
Rp2h
BB431808
1.53
0.48

1415957_a_at
Nnp1
AV297071
1.59
0.83
1424244_at
Rwdd4a
AK005797
1.53
1.30

1416479_a_at
Tmem14c
NM_025387
1.59
0.68
1425014_at
Nr2c2
AU066920
1.53
1.11

1430274_a_at
Stard3nl
AK018331
1.59
1.01
1425713_a_at
Rnf146
BC019182
1.53
1.30

1450213_at
Pde7b
NM_013875
1.59
1.52
1450184_s_at
Tef
NM_017376
1.53
1.00

1419650_at
Zfr
NM_011767
1.59
0.88
1439764_s_at
Igf2bp2
BB098431
1.53
1.20

1420838_at
Ntrk2
AK018789
1.59
1.58
1438473_at
Arl15
BB445175
1.53
0.97

1428461_at
Ppp2r5e
BB535888
1.59
1.12
1440886_at
Cdc37l1
BE949427
1.53
1.65

1436763_a_at
Klf9
AI267126
1.59
0.84
1451622_at
Lmbrd1
BM206793
1.53
1.21

1417818_at
Wwtr1
BC014727
1.58
0.97
1429265_a_at
Rnf130
AK011088
1.53
0.93

1420514_at
Tmem47
NM_138751
1.52
1.12
1450744_at
Ell2
NM_138953
1.48
0.92

1419444_at
Sap18
NM_009119
1.52
1.11
1423503_at
Jam3
AK017692
1.47
1.32

1421867_at
Nr3c1
NM_008173
1.52
0.94
1448376_at
Wrnip1
NM_030215
1.47
1.11

1455020_at
Snx25
AV351081
1.52
1.14
1419580_at
Dlg4
AI646416
1.47
0.58

1427311_at
Bptf
BB380312
1.52
1.07
1424749_at
Wdfy1
BC025226
1.47
1.32

1451018_at
Leprotl1
BF658789
1.52
1.15
1427427_at
Ryr3
AV238793
1.47
1.13

1420833_at
Vamp2
BG871810
1.52
1.26
1431020_a_at
Fgfr1op2
AK008983
1.47
0.95

1450174_at
Ptprt
NM_021464
1.52
1.25
1416123_at
Ccnd2
NM_009829
1.47
1.55

1425563_s_at
Pcdh10
AF334801
1.51
1.48
1422621_at
Ranbp2
BM507707
1.47
1.53

1428523_at
Lphn3
BE945410
1.51
0.98
1434877_at
Nptx1
AI152800
1.47
1.19

1450907_at
Spcs2
BI078449
1.51
0.86
1420849_at
Crnkl1
AV143435
1.47
1.06

1437318_at
Pak3
BB468082
1.51
0.99
1420661_a_at
4933439F18Rik
NM_025757
1.47
1.64

1448896_at
Pigf
NM_008838
1.51
1.08
1426491_at
Herc2
AW701798
1.47
1.43

1422607_at
Etv1
NM_007960
1.51
1.36
1428998_at
Phf3
AK010013
1.47
0.93

1452757_s_at
Hba-a1
AK011116
1.51
1.16
1444015_at
Papola
AV307016
1.47
0.76

1423060_at
Pa2g4
BM232515
1.51
0.79
1434353_at
Sfmbt2
BM200222
1.46
0.55

1438407_at
Dsel
AV336691
1.51
0.95
1417538_at
Slc35a1
NM_011895
1.46
0.84

1426301_at
Alcam
U95030
1.51
0.58
1445843_at

BE199465
1.46
0.82

1450668_s_at
Hspe1
NM_008303
1.50
1.07
1419914_s_at
D10Ertd438e
AW538011
1.46
1.25

1418318_at
Rnf128
AK004847
1.50
0.88
1436788_at
Acp2
AI131584
1.46
1.04

1429857_at
Pou3f3
BB807002
1.50
1.22
1419392_at
Pclo
NM_011995
1.46
1.14

1453380_a_at
Xrcc6bp1
AK010471
1.50
1.57
1421990_at
Syt1
NM_009306
1.46
1.30

1426258_at
Sorl1
BI648081
1.50
1.13
1416133_at
C920006C10Rik
BB188557
1.46
1.18

1432287_a_at
Sntg1
AK016927
1.50
0.92
1416726_s_at
Ube2s
NM_133777
1.46
0.97

1419035_s_at
Csnk2a1
BB283759
1.50
1.08
1417924_at
Pak3
BQ174935
1.46
1.12

1425929_a_at
Rnf14
AF249668
1.50
1.06
1420165_s_at
Dnajc17
N28111
1.46
0.90

1447771_at

AW050081
1.50
0.49
1418084_at
Nip1
AK011144
1.46
1.60

1450120_at
Scn1a
AV336781
1.50
1.12
1423753_at
Bambi
AF153440
1.46
0.94

1460061_at
Adra1a
AV342748
1.50
1.06
1430134_a_at
Yars2
AK008774
1.46
1.19

1421353_at
Pde7b
NM_013875
1.50
1.29
1425060_s_at
Clip1
BG969810
1.45
1.15

1431094_at
1110006E14Rik
AK003491
1.50
0.81
1425855_a_at
Crk
AF239673
1.45
1.05

1434937_at
Phr1
AW546647
1.50
0.96
1428620_at
Ensa
BF584204
1.45
1.24

1429330_at
Gabra4
AK013727
1.50
0.95
1417878_at
E2f1
NM_007891
1.45
1.33

1450051_at
Atrx
BB825830
1.50
0.86
1429336_at
Tmem87b
BB161454
1.45
0.97

1452714_at
Tanc1
AK004560
1.49
1.07
1423211_at
Nola3
AK004120
1.45
1.09

1427988_s_at
Safb2
BM239918
1.49
1.30
1460336_at
Ppargc1a
BB745167
1.45
0.95

1452335_at
2810423E13Rik
AV024565
1.49
0.72
1416266_at
Pdyn
AF026537
1.45
1.76

1429045_at
Smurf2
BI697997
1.49
1.09
1437366_at
AI608492
BB332542
1.45
1.02

1436451_a_at
Tmed2
AW824211
1.49
1.06
1438452_at
Nebl
BM121794
1.45
0.86

1425132_at
Neto1
AF448840
1.49
1.15
1452894_at
Elavl4
AK013588
1.45
0.54

1435226_at
Ibrdc3
BG064140
1.49
1.23
1426885_a_at
Cdk2ap1
AK004852
1.45
1.06

1430780_a_at
Pmm1
BI739353
1.49
0.80
1424886_at
Ptprd
BC025145
1.45
0.94

1434736_at
Hlf
BB744589
1.49
1.21
1434956_at
AI481227
AV145226
1.45
0.85

1437481_at
1110039F03Rik
AV032349
1.49
0.93
1452813_a_at
5033428A16Rik
AK017198
1.45
1.33

1426300_at
Alcam
U95030
1.49
0.84
1455309_at
Tmem16f
BG074632
1.45
1.15

1434386_at
Atp2c1
BM225269
1.49
1.11
1419398_a_at
Reep5
NM_007874
1.45
0.96

1434206_s_at
Ppp2r5c
BM247370
1.48
1.34
1429475_at
2810457I06Rik
AK013361
1.45
2.15

1437560_at
Ntrk2
BQ174783
1.48
1.26
1428237_at
2700059D21Rik
BI689227
1.44
1.15

1450569_a_at
Rbm14
NM_019869
1.48
1.22
1452271_at
Xpr1
AV337591
1.44
1.10

1448346_at
Cfl1
NM_007687
1.48
0.97
1457293_at

BF714580
1.44
1.01

1455365_at
Cdh8
BB426483
1.48
1.04
1426613_a_at
Snrpb2
AV066554
1.44
1.12

1426455_at
Sdccag10
AK014025
1.48
0.84
1426310_at
Zdhhc5
AV241525
1.44
1.68

1426397_at
Tgfbr2
BG793483
1.48
1.37
1455215_at
C530028O21Rik
AU067746
1.44
1.04

1433772_at
Stch
BE650268
1.48
1.23
1427308_at
Dab1
BB64109
1.44
1.04

1451147_x_at
Csdc2
BC016109
1.48
1.38
1434306_at
Rab3ip
BF19015
1.44
1.33

1436473_at
Zfp248
AV300441
1.44
0.48
1416711_at
Tbr1
NM_009322
1.41
1.19

1420922_at
Usp9x
AW107303
1.44
0.93
1453264_at
Marveld3
AK007346
1.41
0.58

1432435_s_at
C030004A17Rik
AK012261
1.44
1.35
1454771_at

AW060701
1.41
1.21

1438975_x_at
Zdhhc14
AV361868
1.44
1.44
1416510_at
Mrpl4
NM_023167
1.41
1.13

1416328_a_at
Atp6v0e
NM_025272
1.44
1.15
1448344_at
Rps12
NM_011295
1.41
0.93

1419397_at
Pola1
NM_008892
1.44
1.03
1451572_a_at
5230400G24Rik
BC016597
1.41
1.00

1416288_at
Dnaja1
BF141076
1.44
1.06
1418367_x_at
Hist2h2aa1
BC010564
1.41
1.08

1429564_at
Pcgf5
AK002387
1.44
1.35
1425780_a_at
Tmem167
BC024352
1.41
0.98

1439573_at
Rtn4rl2
BE992565
1.44
2.15
1455632_at
Gnb5
BE446953
1.41
1.75

1438258_at
Vldlr
BE647363
1.43
1.21
1416567_s_at
Atp5e
NM_025983
1.41
1.20

1460441_at
Zxdb
BF660409
1.43
0.98
1418469_at
Nrip1
NM_008735
1.41
1.40

1417106_at
Tpd52l2
NM_025482
1.43
1.22
1418386_at
N6amt2
NM_026526
1.41
1.15

1425480_at
Cnot6l
BC018506
1.43
1.00
1453077_a_at
Snapc3
AK016168
1.41
0.97

1428184_at
3110035E14Rik
BB348639
1.43
1.21
1433868_at
Btbd3
AV028445
1.41
1.09

1436985_at
Zfp644
BM115349
1.43
0.69
1434604_at
Eif5b
BM236870
1.41
1.16

1439847_s_at
Klf12
BM249597
1.43
1.12
1437118_at
Usp7
C77542
1.41
1.04

1450773_at
Kcnd2
BB051684
1.43
0.86
1453406_a_at
Rab28
AK012286
1.41
1.12

1451270_at
Dusp18
BC020036
1.43
1.00
1435553_at
Pdzd2
AV376136
1.40
1.47

1450949_at
Katna1
AK012319
1.43
0.80
1416214_at
Mcm4
BC013094
1.40
0.82

1419169_at
Mapk6
BC024684
1.43
1.11
1449507_a_at
Cd47
NM_010581
1.40
1.01

1437021_at
Arl13b
AV225959
1.43
0.91
1457587_at
Ga17
BB196645
1.40
0.97

1448882_at
Tmem93
NM_025318
1.43
1.08
1416566_at
Strap
NM_011499
1.40
1.02

1429488_at
Zdhhc21
AK017682
1.43
1.07
1417612_at
Ier5
BF147705
1.40
1.18

1436169_at
C730029A08Rik
BB428892
1.43
1.21
1460174_at
Dexi
NM_021428
1.40
1.23

1455944_at
Zfp516
BB467812
1.43
0.78
1423172_at
Napb
BB147418
1.40
0.89

1436796_at
1110061A14Rik
BB237072
1.43
0.83
1449324_at
Ero1l
BM234652
1.40
1.21

1419280_at
Pip5k2a
AK012196
1.43
0.70
1423477_at
Zic1
BB361162
1.40
0.79

1420537_at
Kctd4
NM_026214
1.43
0.86
1452905_at
Gtl2
AV015833
1.40
0.68

1418927_a_at
Habp4
NM_019986
1.43
1.05
1442257_at
Cdh6
BI134319
1.40
1.56

1434791_at
Atp6v0a2
BM229554
1.43
1.45
1417933_at
Igfbp6
NM_008344
1.39
1.65

1454987_a_at
H2-Ke6
A1323545
1.43
1.10
1453286_at
Plxna2
BB085537
1.39
1.64

1441371_at
Plxna4
BQ174019
1.43
0.93
1429779_at
Eif2c4
A1481660
1.39
1.47

1442051_at
Hist2h3c1
BE691662
1.43
1.19
1419127_at
Npy
NM_023456
1.38
1.65

1427683_at
Egr2
X06746
1.43
1.27
1435891_x_at
2610021A01Rik
BQ175465
1.38
0.53

1433532_a_at
Mbp
AI323506
1.43
1.20
1441961_at
Mtap9
BB698742
1.37
0.68

1436868_at
Rtn4rl1
BM508396
1.43
1.14
1452370_s_at
B230208H17Rik
BB449608
1.37
0.59

1442027_at
Nbeal1
BB223743
1.43
1.56
1454701_at
4930503L19Rik
AI450962
1.37
0.59

1423316_at
Tmem39a
AK017817
1.42
0.91
1424373_at
Armcx3
AK004598
1.37
1.45

1426743_at
Appl2
BC002232
1.42
1.21
1418003_at
1190002H23Rik
NM_025427
1.36
1.78

1431024_a_at
Arid4b
AK020165
1.42
0.54
1433733_a_at
Cry1
BG069864
1.36
1.57

1453787_at
Txndc13
AK015667
1.42
1.10
1434202_a_at
BC055107
BF682848
1.35
1.42

1431711_a_at
9030409G11Rik
AK018497
1.42
1.88
1416316_at
Slc27a2
BC013442
1.35
0.58

1422968_at
Ihpk1
NM_013785
1.42
0.91
1423278_at
Ptprk
AI893646
1.35
1.73

1421193_a_at
Pbx3
NM_016768
1.42
1.01
1456685_at
Nsg2
BB227199
1.34
1.75

1423198_a_at
Smek2
AV305633
1.42
0.88
1445669_at
Spry4
BB080456
1.34
2.12

1422517_a_at
Znrd1
NM_023162
1.42
0.88
1440437_at
Herc1
BM247513
1.34
0.51

1423501_at
Max
AA617392
1.42
0.95
1458358_at
Pank2
BB402666
1.34
0.41

1451486_at
1200006F02Rik
BC006902
1.42
0.86
1449885_at
Tmem47
NM_138751
1.34
1.69

1419954_s_at
Zfand3
AW539211
1.42
1.02
1433864_at
Lrp12
AV254798
1.34
1.51

1435244_at
Vav2
BF535947
1.42
0.92
1456388_at
Atp11a
AV378604
1.34
1.72

1437614_x_at
Zdhhc14
AV223474
1.42
1.42
1416630_at
Id3
NM_008321
1.33
1.61

1437858_at
Dpy19l3
AV367203
1.42
1.05
1427134_at
Sfrs12
AV012790
1.33
1.41

1455076_a_at
4933424B01Rik
AV255808
1.42
0.80
1429150_at
Jarid1a
BM224226
1.33
1.52

1455113_at
Armc8
BM232782
1.42
1.01
1452868_at
Usp24
AK012842
1.33
1.66

1435451_at
Hel308
BG068026
1.42
1.54
1447275_at
Bbs12
AI449447
1.33
1.65

1435616_at
Cyp20a1
BE686557
1.33
1.51
1424169_at
Tax1bp3
BC008166
1.21
0.50

1457670_s_at
Lmna
AV238225
1.33
0.61
1426373_at
Ski
AK019148
1.21
1.42

1446090_at

BB521597
1.32
1.50
1415840_at
Elovl5
NM_134255
1.21
1.43

1416432_at
Pfkfb3
NM_133232
1.32
1.56
1426047_a_at
Ptprr
AF129509
1.21
0.71

1453172_at
Stch
BE533039
1.32
1.41
1453166_at
Ccdc109a
AK008216
1.20
0.64

1435156_at
BC046331
BF730275
1.32
1.55
1421148_a_at
Tial1
NM_009383
1.20
0.68

1419182_at
Svep1
NM_022814
1.31
1.90
1427064_a_at
Scrib
BC006859
1.20
1.41

1456946_at
Sh3md4
BE949337
1.31
1.55
1426434_at
Tmem43
AI747289
1.20
0.47

1423104_at
Irs1
BB345784
1.30
1.45
1440504_at
AI844750
BB397002
1.20
0.50

1419453_at
Uchl5
AV313813
1.30
0.50
1451525_at
Arhgap12
BG064038
1.19
0.69

1423337_at
Orc4l
BB775020
1.29
0.71
1417675_a_at
Mdn1
NM_133874
1.19
1.53

1428083_at
2310043N10Rik
AK018202
1.29
1.44
1420845_at
Mrps2
AV031454
1.19
1.43

1449913_at
Zfp2
NM_009550
1.29
0.67
1422675_at
Smarce1
AK018493
1.19
0.69

1455876_at
Slc4a7
BM237826
1.29
0.55
1420485_at
Nol7
AW060738
1.19
0.66

1436103_at
Rab3ip
AV235634
1.29
1.44
1460300_a_at
Ltk
NM_008523
1.19
1.54

1436758_at
Hdac4
AI661423
1.29
1.43
1418595_at
S3-12
NM_020568
1.19
2.42

1422320_x_at
Phxr5
NM_008836
1.28
3.21
1459917_at
Ggnbp2
BG078867
1.19
0.64

1436137_at
Slc6a17
AV340147
1.28
1.65
1428340_s_at
Atp13a2
BM944122
1.19
1.45

1426852_x_at
Nov
X96585
1.28
1.77
1454962_at
Spire1
AU067702
1.18
1.46

1456753_at

AW209075
1.28
1.41
1423475_at
Cnnm2
BB278418
1.18
1.46

1457693_a_at
6430537H07Rik
AV343405
1.28
2.14
1438724_at
Osbpl3
AI875442
1.18
1.41

1418966_a_at
Dcbld1
AK014521
1.28
1.64
1438838_at
B230206F22Rik
BG141977
1.18
0.49

1455164_at
Cdgap
AV308092
1.28
1.42
1440688_at
Arhgap26
BM244666
1.18
1.60

1455518_at

BB324402
1.27
1.45
1435304_at
Sod1
BM240246
1.18
0.71

1454014_a_at
Mkks
AK004987
1.26
1.57
1424160_at
Alg5
BC027160
1.18
0.59

1418587_at
Traf3
U21050
1.26
1.53
1426886_at
Cln5
AV315220
1.18
1.48

1448569_at
Cd8b1
BF682469
1.26
1.47
1439615_at
Gan
BB187898
1.18
1.49

1422533_at
Cyp51
NM_020010
1.26
1.48
1454693_at
Hdac4
BQ176116
1.18
1.48

1435229_at
Gramd1b
BI729743
1.26
1.42
1455260_at
Lcorl
BE650208
1.17
0.63

1439019_at
Fras1
BB541392
1.26
1.73
1422910_s_at
Smc6
AU022584
1.17
0.57

1420807_a_at
Egfl9
NM_134120
1.25
1.48
1449351_s_at
Pdgfc
NM_019971
1.17
1.48

1457694_at
6430537H07Rik
AV343405
1.25
1.71
1423269_a_at
Nedd4l
BB663717
1.17
1.40

1417219_s_at
Tmsb10
NM_025284
1.25
1.52
1435402_at
Gramd1b
BB066186
1.17
1.51

1440308_at
Cmpk
AV347071
1.25
0.67
1444359_at
Zfp777
BB354954
1.17
0.51

1460426_at
Pde4dip
AI639670
1.25
1.47
1451227_a_at
Slc10a3
BC027440
1.17
1.47

1422165_at
Pou3f4
X66603
1.24
1.44
1427004_at
Fbxo2
BB311718
1.17
1.61

1436588_at
Rttn
BB700837
1.24
1.53
1430986_at
Farslb
AK012154
1.17
2.67

1427035_at
Slc39a14
BB399837
1.24
1.46
1453556_x_at
Cd99
AK002762
1.17
1.43

1432431_s_at
2900006F19Rik
AK004072
1.24
1.61
1457948_at

BF456094
1.16
0.54

1451753_at
Plxna2
D86949
1.24
1.69
1427287_s_at
Itpr2
BB216794
1.16
1.62

1452123_s_at
Frmd4b
BG067753
1.24
1.48
1419672_at
Spock1
BB308491
1.16
0.59

1420579_s_at
Cftr
NM_021050
1.23
1.89
1437252_at
Gats
BB768012
1.16
1.43

1437291_at
2700081O15Rik
AV131166
1.23
0.55
1455086_at
Uchl5
AV312620
1.16
0.47

1424590_at
Ddx19b
BC002300
1.23
1.55
1427261_at
Wwc1
BQ176786
1.16
1.44

1427902_at
Srrm2
BQ176063
1.23
0.63
1456256_at
Eif5
BM242785
1.16
1.66

1431541_at
Stt3b
AK016943
1.23
1.89
1419380_at
Zfp423
NM_033327
1.16
1.56

1420530_at
Neud4
AW553317
1.23
0.57
1435642_at
Sft2d1
BB081437
1.16
1.51

1438007_at
AI851790
BB432758
1.22
0.58
1446357_at
BC020402
BB029175
1.16
0.71

1427343_at
Rasd2
BC026377
1.22
1.82
1455150_at

BB038506
1.16
0.63

1457440_at
Sstr4
BB451927
1.22
1.40
1455601_at

BB507994
1.16
0.69

1441396_at
B3galt1
AV328619
1.22
1.76
1421819_a_at
Set
BF134272
1.15
0.68

1450684_at
Etv1
NM_007960
1.22
1.53
1427785_x_at
Solh
BC022685
1.15
1.41

1437049_at

BE627361
1.21
0.51
1428166_at
Cdan1
AK005239
1.15
1.51

1448918_at
Slco3a1
NM_023908
1.21
1.69
1416246_a_at
Coro1a
BC002136
1.15
1.65

1423137_at
Rala
BG073338
1.21
0.64
1427542_at
5330439J01Rik
BC027318
1.15
1.74

1454824_s_at
Pdgfrl
BB699957
1.15
1.44
1455622_at
Podxl2
BB461988
1.10
1.90

1417379_at
Iqgap1
NM_016721
1.15
1.46
1416444_at
Elovl2
NM_019423
1.10
1.76

1425731_at
Ankrd24
BC017633
1.15
0.55
1435031_at
2010310D06Rik
AV069499
1.10
1.53

1415772_at
Ndl
BF118393
1.15
0.58
1453434_at
1110019D14Rik
AK003812
1.10
1.86

1423213_at
Plxnc1
BB476707
1.15
1.46
1416683_at
Plxnb2
NM_138749
1.10
1.52

1438011_at
Pcyt1a
BB280291
1.15
1.50
1437983_at
Sall1
BB739342
1.10
1.76

1434505_a_at
6430548M08Rik
BF465109
1.15
2.11
1455014_at
AV009015
BM213104
1.10
0.66

1451474_a_at
Parp8
BC022679
1.14
2.13
1449476_at
Rage
NM_011973
1.10
0.71

1454878_at
2310047C04Rik
BM936291
1.14
0.57
1453500_at
Cyp2u1
AK018458
1.10
1.58

1439380_x_at
Gtl2
BB093563
1.14
0.66
1434826_at
Rfesd
AW124912
1.09
0.63

1419757_at
Pitpnm2
NM_011256
1.14
1.77
1417216_at
Pim2
NM_138606
1.09
0.71

1448655_at
Lrp1
NM_008512
1.14
1.47
1425067_at
Celsr3
AF427498
1.09
1.73

1434215_at
B230308N11Rik
AW554529
1.14
1.54
1436030_at
Cachd1
BB730977
1.09
1.82

1441636_at

BB133117
1.14
1.43
1426979_at
Mlxip
AF265663
1.09
1.56

1452199_at
2700094F01Rik
BB667255
1.14
0.68
1454136_a_at
4921524J17Rik
AK013123
1.09
0.62

1416803_at
Fkbp7
NM_010222
1.13
0.56
1456697_x_at
Dintf1
BB248138
1.09
2.03

1425732_a_at
Mxi1
D31824
1.13
1.50
1451156_s_at
Vldlr
BB628702
1.09
0.70

1436515_at
E030004N02Rik
BB529913
1.13
1.98
1422742_at
Hivep1
NM_007772
1.09
1.74

1426577_a_at
1810054G18Rik
BM570079
1.13
1.45
1455448_at
Nt5dc3
BG804066
1.08
1.41

1437813_at
Aim1l
BE948330
1.13
0.66
1423560_at
Nell2
AI838010
1.08
1.93

1432156_a_at
Rnf32
AK012288
1.13
1.56
1454036_a_at
Usp15
AK014891
1.08
1.49

1417312_at
Dkk3
AK004853
1.13
1.71
1453246_at
Rab39b
AK020665
1.08
0.65

1427978_at
4732418C07Rik
BB318254
1.13
1.50
1454606_at
4933426M11Rik
BF144564
1.08
1.42

1436630_at
Aqp11
AI550443
1.13
1.49
1421191_s_at
Gopc
NM_053187
1.08
1.50

1428301_at
LOC554327
BM195235
1.12
1.50
1451332_at
Zfp521
BC021376
1.08
1.47

1456316_a_at
Acbd3
BI965035
1.12
0.30
1442739_at
BC031441
BF225404
1.07
0.55

1440930_a_at
Scrt2
BG807042
1.12
0.68
1449390_at
Gpatc4
NM_025663
1.07
1.63

1429984_at
5730455O13Rik
AK017666
1.12
0.49
1454756_at
Lrch3
BB763476
1.07
2.17

1424523_at
Elmo1
BCO24727
1.12
1.59
1417303_at
Mvd
NM_138656
1.07
0.69

1454720_at
Hs3st5
AV328620
1.12
1.64
1417551_at
Cln3
NM_009907
1.07
1.52

1418025_at
Bhlhb2
NM_011498
1.12
1.66
1437020_at
Ep400
BM236717
1.07
0.43

1439561_at
2010012O05Rik
BB322051
1.12
0.51
1416589_at
Sparc
NM_009242
1.07
1.71

1418045_at
Inpp1
NM_008384
1.12
1.44
1440972_at
Nsd1
AI413236
1.07
0.64

1427209_at
Baz2a
AW910654
1.12
0.66
1442220_at

BB313387
1.07
1.53

1443493_at
Dhx37
BB766805
1.12
0.46
1418313_at
Zfp276
BB667131
1.07
1.50

1417567_at
Ctnnbip1
BF457754
1.11
1.45
1415874_at
Spiy1
NM_011896
1.06
1.52

1420934_a_at
Srrm1
NM_016799
1.11
0.58
1415758_at
Fryl
BM118442
1.06
1.48

1426594_at
Frmd4b
BG067753
1.11
1.47
1433682_at
Arhgef17
BE287052
1.06
1.42

1450661_x_at
Nfic
NM_008688
1.11
1.64
1442917_at

BM195384
1.06
1.63

1452306_at
Zfyve26
BC006654
1.11
1.45
1438363_at
EG434128
BB332375
1.06
1.57

1415692_s_at
Canx
BI653492
1.11
0.70
1440215_at
″OTTMUS




1416049_at
Gldc
NM_138595
1.11
1.51
G0000000446
BG068916
1.06
0.62


1435952_at
Tsc22d1
AV118079
1.11
1.93
1″





1425492_at
Bmpr1a
BM939768
1.11
1.43
1435737_a_at
Nde1
BQ032232
1.05
1.45

1429489_at
Rexo1
AK012127
1.11
1.74
1437891_at
Frs2
AV331887
1.05
1.55

1420925_at
Tub
NM_021885
1.11
1.45
1444072_at

AW123332
1.05
0.59

1448396_at
Tmem131
NM_018872
1.11
1.48
1429440_at
1810041L15Rik
BI734299
1.05
1.74

1455153_at
Zfp236
BQ177220
1.11
1.53
1435527_at
Nfic
BB533448
1.05
1.42

1419362_at
Mrpl35
BF787384
1.11
0.53
1440291_at

BE954335
1.05
0.62

1450135_at
Fzd3
AU043193
1.10
0.62
1416187_s_at
Pnrc2
NM_026383
1.05
0.68

1429224_at
Pnmal
AK017476
1.10
0.70
1442754_at
C030013G03Rik
BE692283
1.05
1.99

1451362_at
Rab7l1
BC016133
1.10
1.49
1443770_x_at
Auts2
BB026407
1.05
1.99

1455520_at
LOC629605
AV327200
1.10
2.74
1428466_at
Chd3
AK011183
1.05
1.50

1436302_at
Slc10a7
BB770006
1.10
1.55
1448733_at
Bmi1
M64279
1.05
0.63

1435488_at

BB427289
1.10
1.68
1460001_at
Pdpep1
AI642171
1.05
0.66

1430814_at
1300013D18Rik
AK004984
1.05
1.43
1452029_a_at
Purg
AF479673
0.99
1.54

1447550_at
Zfp644
BB545511
1.05
0.62
1429185_at
4631416L12Rik
BB774721
0.99
1.81

1449349_at
Nudt1
NM_008637
1.04
0.61
1420375_at
Kif3a
NM_008443
0.99
0.59

1421252_a_at
Mef2a
NM_013597
1.04
0.63
1435810_at
5730455O13Rik
AV297833
0.99
0.71

1434654_at
Cog3
BE570422
1.04
1.50
1444736_at
Cdh7
BB129525
0.99
1.48

1439783_at
C330018D20Rik
BB809870
1.04
0.68
1421392_a_at
Birc3
NM_007464
0.98
1.72

1422635_at
Ache
NM_009599
1.04
2.16
1424138_at
Rhbdf1
BC027346
0.98
1.42

1425423_at
Glis1
AF220434
1.04
0.61
1452347_at
Mef2a
AV255689
0.98
0.68

1435719_at
AI448984
BM935216
1.04
0.62
1417870_x_at
Ctsz
NM_022325
0.98
1.50

1424042_at
Tmem5
BC020100
1.04
0.67
1435335_a_at
Gnptab
BG144467
0.98
1.42

1454198_a_at
Efcab1
AK015866
1.04
0.58
1439506_at
Gm98
BB193557
0.98
1.49

1439817_at
2900064A13Rik
AI451465
1.04
0.70
1447230_at
AI553587
BE954466
0.98
0.63

1428403_at
2410025L10Rik
AV330483
1.04
1.95
1455293_at
Leo1
BG065311
0.98
0.63

1455333_at
Tns3
AI315031
1.03
1.43
1416806_at
Fdxr
NM_007997
0.97
0.70

1455700_at
Mterfd3
BB359043
1.03
0.65
1430776_s_at
Ankrd24
AK017778
0.97
0.66

1427172_at
Ofd1
AJ278702
1.03
0.49
1436200_at
Lonrf3
BE956940
0.97
1.52

1428431_at
2310047A01Rik
BB813478
1.03
1.57
1428794_at
Specc1
BQ175052
0.97
1.55

1460394_a_at
Inppl1
BB769433
1.03
1.47
1434600_at
Tjp2
BB758095
0.97
1.49

1455275_at
E530001K10Rik
AW046441
1.03
0.45
1428732_at
1700008J07Rik
AK005774
0.97
0.61

1456520_at
Zfp652
BF147545
1.03
1.57
1426865_a_at
Ncam1
BB698413
0.97
0.56

1435295_at
Dopey1
BG075614
1.03
1.45
1422612_at
Hk2
NM_013820
0.97
1.52

1422324_a_at
Pthlh
NM_008970
1.03
1.63
1436032_at

BB201888
0.97
1.96

1435783_at
B230112C05Rik
BB476773
1.03
0.69
1426848_at
Sec24b
BE947982
0.97
1.49

1458307_at
B230334C09Rik
BE992834
1.03
0.51
1431072_a_at
Ccdc50
BB865814
0.97
1.59

1426405_at
Rnf11
BI150320
1.02
0.69
1455906_at
6030446N20Rik
AV222909
0.97
0.59

1455758_at
Prkcc
BM215011
1.02
1.62
1439558_at
Zfp75
BM729399
0.97
0.68

1425118_at
Spire2
BC026502
1.02
1.68
1443528_at
Ttbk1
BB398560
0.97
1.55

1452267_at
Flywch1
BB477613
1.02
0.49
1425968_s_at
Speg
AF215896
0.96
1.52

1429684_at
5830472M02Rik
BG094398
1.02
2.23
1452604_at
Stard13
BB667840
0.96
1.47

1421830_at
Ak3l1
NM_009647
1.02
1.48
1426316_at
6330416G13Rik
AV326978
0.96
0.71

1450937_at
Lin7c
BQ176612
1.02
0.65
1427269_at
Sfrs11
AW261583
0.96
0.61

1442415_at
5830454E08Rik
BQ180241
1.02
0.64
1434952_at
Cox4nb
BI734783
0.96
1.45

1417577_at
Trpc3
NM_019510
1.02
0.56
1444208_at
Dnahc1
BE950899
0.96
0.57

1421586_a_at
Cd46
NM_010778
1.02
0.62
1436707_x_at
Ncaph
BB725358
0.96
0.70

1434691_at
Sfrs2ip
BB154529
1.02
0.71
1428223_at
Mfsd2
AK006096
0.96
1.82

1448554_s_at
Myh7
NM_080728
1.02
0.69
1438476_a_at
Chd4
BB201828
0.96
1.49

1457544_at
Ube2w
BB442807
1.01
0.59
1417384_at
Entpd5
NM_007647
0.96
1.97

1429783_at
Pdlim5
AK009464
1.01
0.48
1416953_at
Ctgf
NM_010217
0.96
1.62

1423738_at
Oxa1l
BC027191
1.01
1.40
1438478_a_at
Ppp3ca
AI313926
0.96
0.48

1425405_a_at
Adar
AF291876
1.01
0.71
1428198_at
Adal
AK016299
0.95
0.67

1449248_at
Clcn2
NM_009900
1.01
0.70
1420402_at
Atp2b2
NM_009723
0.95
1.97

1429836_at
Ugcgl2
BM218963
1.01
1.93
1452055_at
Ctdsp1
BB770944
0.95
1.51

1428352_at
Arrdc2
AW542672
1.01
0.49
1439516_at
2610201A13Rik
AW488471
0.95
0.66

1451625_a_at
C8g
BC019967
1.00
1.41
1433890_a_at
Bat3
AW545877
0.95
1.46

1444001_at
Strbp
AW488249
1.00
0.52
1417937_at
Dact1
NM_021532
0.95
1.61

1452661_at
Tfrc
AK011596
1.00
0.71
1418924_at
Rassf7
NM_025886
0.95
0.68

1458492_x_at
Hnt
BB081359
1.00
0.67
1452831_s_at
Ppat
AV305746
0.95
0.67

1440884_s_at
A530047J11Rik
BB218047
1.00
0.55
1419905_s_at
Hpgd
AV026552
0.94
0.45

1419047_at
Pcnx
BG073499
1.00
1.43
1424125_at
Kcnk13
BC023443
0.94
1.49

1423097_s_at
Capn7
BQ257745
1.00
0.71
1429499_at
Fbxo5
AK011820
0.94
0.70

1455497_at
Leng9
BB799608
1.00
0.67
1442434_at
D8Ertd82e
BM195829
0.94
1.40

1436165_at
Luc7l2
BI076494
1.00
0.63
1435324_x_at
Hmgb1
AI648759
0.94
1.50

1458337_at
Tcf12
BB476667
0.99
0.60
1448546_at
Rassf3
BB703307
0.94
0.63

1456858_at
Gpr149
BB075339
0.99
0.60
143989_at
2900054J07Rik
BB326106
0.94
0.51

1438469_at
Bcorl1
BG071122
0.99
1.42
1435617_at
1700106N22Rik
BQ175501
0.94
0.40

1452338_s_at
Itsn1
AA172344
0.94
2.52
1460520_at
Mocs1
AK014178
0.89
0.67

1428870_at
Nolc1
BM213850
0.94
0.68
1427544_a_at
Papola
U58135
0.89
0.68

1439463_x_at
EG666836
AV127023
0.94
1.48
1448145_at
Wwp2
AK004087
0.89
0.70

1439909_at
Zfp521
BE133660
0.94
1.51
1428773_s_at
Bcor
AK018370
0.89
1.41

1425094_a_at
Lhx6
AB031040
0.94
2.01
1424687_at
2700008B19Rik
BF150550
0.89
1.79

1430435_at
Aff3
AK014322
0.94
1.96
1419292_at
Htra3
NM_030127
0.89
0.68

1439684_at
4930570G19Rik
BB428007
0.94
0.63
1417194_at
Sod2
NM_013671
0.89
0.59

1455463_at
Phyhip
BB427286
0.94
0.60
1429573_at
Dmrtc1a
AK014934
0.89
0.70

1422725_at
Mak
BG069426
0.94
1.48
1457811_at

BM217632
0.89
1.52

1457536_at
Gpc5
BB171986
0.94
2.03
1415680_at
Anapc1
NM_008569
0.89
1.45

1421892_at
St3gal2
BB750118
0.93
1.42
1443210_at

BB354985
0.88
0.62

1422881_s_at
Sypl
BE333485
0.93
0.69
1434762_at
Tmem142b
BF457736
0.88
0.66

1431820_at
4632404H12Rik
AK014549
0.93
0.66
1424571_at
Ddx46
BF023426
0.88
0.50

1449857_at
1200011I18Rik
NM_026177
0.93
0.66
1438210_at
Gpr149
BB126999
0.88
0.55

1434610_at
Plec1
BM210485
0.93
1.74
1420391_at
Pard3
NM_033620
0.88
1.70

1426292_at
6330581L23Rik
BG068796
0.93
1.42
1437511_x_at
Clcc1
BB100861
0.88
0.69

1424400_a_at
Aldh1l1
AK007822
0.93
0.71
1434950_a_at
Armc8
BE995635
0.87
0.63

1425391_a_at
Osbpl5
AB074008
0.93
1.46
1440997_at
Tnrc6c
AV236734
0.87
0.58

1420487_at
Nol7
AW060738
0.93
0.63
1452618_at
Cdk5rap2
BM230774
0.87
1.59

1427186_a_at
Mef2a
AV255689
0.93
0.66
1441534_at
C86753
BG067565
0.87
1.81

1435555_at
Cd247
AW552088
0.93
1.42
1456781_at

AW121365
0.87
0.66

1427138_at
0610010D24Rik
AW556861
0.92
1.56
1425489_at
Slu7
BC025870
0.87
0.71

1429087_at
1110054O05Rik
AK005245
0.92
0.67
1427911_at
2610307O08Rik
AK012006
0.87
1.67

1418252_at
Padi2
NM_008812
0.92
0.57
1448417_at
Ninj1
NM_013610
0.87
0.69

1435110_at
Unc5b
BG065285
0.92
1.57
1435438_at
Sox8
AV345303
0.87
0.67

1419313_at
Ccnt1
NM_009833
0.92
1.41
1435456_at
Ttc28
AW552254
0.86
1.46

1433979_at
Rbms2
BQ176636
0.92
1.45
1448384_at
Pofut2
BC018194
0.86
1.52

1439239_at
Lin7b
BB806633
0.92
1.44
1454969_at
Lypd6
AV299725
0.86
1.49

1429517_at
Zfyve20
BC017622
0.92
1.54
1440709_at
C130081A10Rik
BB375586
0.86
0.58

1436726_s_at
Sptlc1
BB470898
0.92
0.69
1417489_at
Npy2r
NM_008731
0.86
1.55

1452127_a_at
Ptpn13
BM236743
0.92
1.70
1440251_s_at
Zfp64
BE947768
0.86
0.61

1421112_at
Nkx2-2
NM_010919
0.92
1.60
1435279_at
BC059842
AV338182
0.85
1.80

1433873_s_at
Pcnt
BG069597
0.91
1.46
1438081_at
Mcc
BB794635
0.85
1.53

1439485_at
Zfp608
AV222442
0.91
1.43
1438951_x_at
Nup54
BB168451
0.85
0.70

1449536_at
Kcnn1
NM_032397
0.91
0.58
1429556_at
2610024B07Rik
BM214769
0.85
1.59

1450097_s_at
Gna12
BF302166
0.91
1.56
1437498_at
Klf9
BB327336
0.85
0.51

1460121_at
9630010G10Rik
BI076710
0.91
0.64
1460333_at
Ddx59
BB829482
0.85
0.63

1424638_at
Cdkn1a
AK007630
0.91
0.57
1443246_at
Fastkd2
BB476363
0.85
0.68

1434085_at
Zfp523
AV136654
0.91
1.41
1457512_at

BB167650
0.85
0.63

1441173_at
4930451G09Rik
AV352442
0.91
0.69
1434596_at

BB194318
0.85
1.56

1437554_at
Plec1
BM232239
0.91
1.62
1419606_a_at
Tnnt1
NM_011618
0.84
0.41

1455991_at
Ccbl2
BG094881
0.91
0.67
1430019_a_at
Hnrpa1
AK007802
0.84
0.70

1459882_at
Asf1a
AV312905
0.91
0.65
1422615_at
Map4k4
NM_008696
0.84
1.58

1416199_at
Kifc3
NM_010631
0.91
1.47
1447344_at

AI447264
0.84
0.71

1458839_at
Exoc8
BB032637
0.90
1.57
1440965_at
Pigl
AW018348
0.84
0.59

1439038_at
9130227C08Rik
BF455240
0.90
0.59
1419048_at
Pcnx
BG073499
0.84
1.70

1420928_at
St6gal1
BG075800
0.90
1.56
1418983_at
Inadl
AV287690
0.84
1.46

1423130_a_at
Sfrs5
AW212917
0.90
0.59
1439060_s_at
Wipi1
BI251603
0.84
0.69

1421478_a_at
Zfp318
NM_021346
0.90
0.63
1450779_at
Fabp7
NM_021272
0.84
0.68

1436524_at
4833438C02Rik
AV252102
0.90
0.63
1419310_s_at
Rfxank
L43164
0.84
0.71

1416536_at
Mum1
NM_023431
0.90
0.63
1429634_at
Zfp580
AK005257
0.84
0.48

1435108_at
Arhgap22
BG295243
0.90
1.42
1435753_a_at
Nucks1
BM209748
0.84
0.63

1436278_at

BM934468
0.90
0.64
1418369_at
Prim1
J04620
0.84
0.67

1426597_s_at
C79267
BG066466
0.90
1.53
1452600_at
Taf6l
AV280284
0.84
0.50

1418534_at
Fzd2
BB371406
0.89
1.45
1455694_at
Nbeal2
C77476
0.83
1.59

1437140_at
Serf2
BB667186
0.83
0.64
1436245_at
Usp20
AV272484
0.71
1.10

1441429_at
Irs4
BB295945
0.83
2.12
1455124_at
Trim68
AW493298
0.71
0.99

1454671_at
Insig1
BB005488
0.83
0.69
1455563_at
Ddx49
BM210028
0.71
0.94

1451042_a_at
Mina
AK013451
0.83
0.62
1457378_at
Mrpl54
AW123022
0.71
1.23

1448602_at
Pygm
NM_011224
0.83
1.45
1419967_at
Seh1l
AW540070
0.70
0.82

1426015_s_at
Asph
AF302653
0.82
0.71
1441000_at
EG237749
BB076832
0.70
0.75

1430135_at
Dnase2a
AK018651
0.81
0.67
1435152_at
Leng8
AV024387
0.70
0.81

1454765_at
Gtf3c3
AW549348
0.81
1.48
1431322_at
Igsf3
AK019524
0.70
0.70

1422793_at
Pafah1b2
BB539054
0.81
0.69
1418070_at
Cdyl
AF081260
0.70
1.27

1438189_s_at
Rai16
BB492312
0.80
2.01
1443372_at

BB417900
0.70
0.97

1422036_at
Strn
NM_011500
0.80
0.68
1445161_at
Usp6nl
BE952061
0.70
0.82

1415728_at
Pabpn1
BG806432
0.80
0.65
1449062_at
Khk
BC013464
0.70
0.87

1433446_at
Hmgcs1
BB705380
0.80
0.69
1439867_at
Ubox5
BB079377
0.70
0.83

1443928_at
Pscdbp
AI462064
0.79
0.56
1422622_at
Nos3
NM_008713
0.70
1.04

1434347_s_at
Rufy2
BB827830
0.79
0.71
1429229_s_at
4930534B04Rik
BE980134
0.70
0.90

1426589_at
Gab3
BB037935
0.79
0.60
1443966_at
C030046E11Rik
BF466594
0.70
0.82

1424721_at
Mfap3
BI661422
0.78
1.45
1429895_at
2310010G23Rik
AK009271
0.70
0.88

1420146_at
Tfb1m
AI429207
0.78
0.70
1446041_at

BB343268
0.70
0.75

1453518_at
Pdzd11
AI429425
0.78
0.52
1424336_at
Ppcdc
BB329391
0.70
0.91

1434749_at
BC067068
BB811762
0.77
0.67
1454727_at
Afap1l1
BB106834
0.70
1.47

1439774_at
Prrx1
BB051738
0.77
2.18
1458667_at
4930519N13Rik
AV266695
0.70
0.64

1452121_at
Fbxo22
BB756840
0.77
0.63
1425891_a_at
Grtp1
AF329833
0.70
1.08

1444461_at
Zmynd11
BB346871
0.77
0.52
1429671_at
2410018M08Rik
AK010551
0.70
1.00

1452228_at
Tbc1d23
AV209678
0.77
0.54
1433023_at
2310068G24Rik
AK010109
0.70
0.98

1427149_at
Plekha6
BB486127
0.77
1.52
1439047_s_at
Recql
BB258011
0.70
0.90

1453163_at
Ppp1r12a
BI653999
0.77
0.48
1445308_at
Tssc1
BB082634
0.70
0.81

1435448_at
Bcl2l11
BM120925
0.77
1.66
1436787_x_at
Sec14l3
AV024133
0.69
0.65

1426226_at
Dyrk1a
U58497
0.76
0.54
1438573_at
Rmi1
AV339477
0.69
0.93

1441993_at
Ap3s2
BB762985
0.76
1.42
1453320_at
1700027A23Rik
AK006407
0.69
0.69

1445564_at

BE688513
0.75
1.45
1441106_at
Zfand5
BB320444
0.69
1.09

1434166_at
9330151L19Rik
BM935724
0.75
0.62
1423747_a_at
Pdk1
BC027196
0.69
1.01

1457195_at
Plekhm1
BB206527
0.74
0.67
1451692_at
Tmco6
BC005637
0.69
0.95

1451809_s_at
Rwdd3
AF439556
0.74
0.52
1424597_at
ORF19
W34605
0.69
0.75

1434423_at
Gulp1
BB138485
0.74
0.62
1441867_x_at
4930534B04Rik
AI480494
0.69
0.71

1442046_at
Shroom2
BM119167
0.74
0.67
1420044_at
Nrd1
AI875733
0.69
0.81

1434695_at
Dtl
AV270035
0.74
1.86
1426230_at
Sphk2
AK016616
0.69
0.71

1448700_at
G0s2
NM_008059
0.74
1.57
1438852_x_at
Mcm6
BB099487
0.69
0.88

1428684_at
1500001M20Rik
BG065846
0.74
0.71
1424955_at
Ccdc5
BC024400
0.68
0.75

1428825_at
Nr6a1
AK007201
0.73
1.43
1433954_at
4632419I22Rik
AV227569
0.68
1.17

1435079_at
5730406M06Rik
BB767442
0.73
0.45
1434326_x_at
Coro2b
BB317923
0.68
0.87

1424839_a_at
Nsun4
BC024628
0.73
0.70
1417920_at
Amn
NM_033603
0.68
0.57

1429796_at
Kalrn
AK008844
0.72
0.67
1418717_at
Mrps25
AK004037
0.68
1.17

1434730_at
AI854517
BB346879
0.72
0.70
1427838_at
Tubb2a
M28739
0.68
0.94

1449800_x_at
Ph17
AI427892
0.71
0.66
1428704_at
Zfp661
BB065039
0.68
1.03

1418984_at
Inadl
AV287690
0.71
1.81
1434591_at
4732460K03Rik
BB004441
0.68
1.18

1428481_s_at
Cdca8
AV307110
0.71
0.77
1445285_at
Hrh3
AV381093
0.68
1.22

1443710_s_at
Spats1
AW045373
0.71
1.04
1418739_at
Sgk2
NM_013731
0.68
1.05

1452805_at
D11Wsu47e
AK017508
0.71
1.21
1455114_at
Ccnu
BB547482
0.68
0.69

1423854_a_at
Rasl11b
BC008101
0.71
0.86
1416429_a_at
Cat
NM_009804
0.68
0.87

1428115_a_at
Rab2b
AK005230
0.71
0.75
1433600_at
Adra2a
BB262415
0.68
1.01

1439645_at
Adra2b
BQ031032
0.71
0.91
1454840_at
Mccc2
BB548059
0.68
0.65

1420059_at
2410166I05Rik
AI480750
0.71
0.85
1444395_at
Dixdc1
AW455436
0.68
1.04

1424742_AT
Creb3
BG070002
0.71
0.88
1435691_at
C630028N24Rik
BB209207
0.68
0.80

1420287_at

R75193
0.71
0.74
1436573_at
Scrn3
BB434447
0.68
0.63

1454614_at
1810013D10Rik
C87702
0.71
0.90
1415877_at
Dpysl3
NM_009468
0.68
0.97

1458281_at
Arsb
BF018283
0.67
0.58
1422307_at

NM_031400
0.62
0.77

1431279_s_at
Ttll5
AK016145
0.67
0.97
1431578_at
Wbp2nl
AK015863
0.62
1.00

1421209_s_at
Ikbkg
NM_010547
0.67
0.95
1430997_at
Cd47
AI882525
0.62
0.76

1417325_at
Btrc
NM_009771
0.67
0.76
1441208_at
Hdhd2
AW546142
0.62
1.10

1417752_at
Coro1c
NM_011779
0.67
1.03
1453607_at
Mfap3l
AV280494
0.61
1.54

1423690_s_at
Gpsm1
BC026486
0.67
0.76
1452328_s_at
Pja2
BF160731
0.61
0.80

1431341_at
Ppp2cb
BF143676
0.67
1.14
1447292_at
Actr1b
BB815306
0.61
1.01

1453061_at
2610018O07Rik
AK011449
0.67
0.71
1458870_x_at
Phr1
BG083658
0.61
1.48

1455048_at
Igsf3
BB484576
0.67
0.91
1456760_at
Centg3
BB342676
0.61
0.94

1428547_at
Nt5e
AV273591
0.67
0.88
1436332_at
Hspb6
BB755506
0.60
0.90

1432391_at
Ccdc21
AK010621
0.67
1.14
1436867_at
Srl
BG795043
0.60
0.72

1438606_a_at
Clic4
BB814844
0.67
0.97
1416164_at
Fbln5
NM_011812
0.60
0.63

1438733_at
Zfp689
BF461546
0.67
1.01
1447544_at

BB486335
0.60
1.06

1447278_at
Cep164
BB822306
0.67
1.08
1421144_at
Rpgrip1
NM_023879
0.60
0.50

1441919_x_at
Crat
BB515871
0.66
1.06
1439609_at

BB536410
0.59
0.86

1423762_at
Adck1
BC010539
0.66
0.81
1456641_at
1190007F08Rik
AV225851
0.59
0.84

1459703_at
D7Ertd443e
BG067408
0.66
0.59
1443813_x_at
Bckdk
AV373435
0.59
0.78

1419069_at
Rabgef1
BB280137
0.66
1.55
1443542_at

BB767151
0.59
0.79

1434987_at
Aldh2
AI462635
0.66
0.85
1420864_at
Zfp161
NM_009547
0.59
0.86

1448246_at
Hdac1
NM_008228
0.66
0.67
1418128_at
Adcy6
NM_007405
0.58
1.65

1460665_a_at
Cnot7
NM_011135
0.66
0.52
1433923_at
Krt77
AV230775
0.58
0.78

1421786_at
Ppp3r1
NM_024459
0.65
1.23
1420913_at
Slco2a1
NM_033314
0.58
0.98

1427491_at
Nsun6
BI108529
0.65
0.93
1437785_at
Adamts9
AV364944
0.57
1.07

1429012_at
Arhgef6
BM246754
0.65
0.67
1431206_at
5730601F06Rik
BI110339
0.57
0.83

1440084_at
AI663975
AV380966
0.65
0.60
1426658_x_at
Phgdh
L21027
0.57
0.81

1440742_at
Epc1
BF019417
0.65
0.91
1417420_at
Ccnd1
NM_007631
0.57
0.92

1434844_at
Hexdc
BF582632
0.65
1.34
1421036_at
Npas2
BG070037
0.57
0.79

1443719_x_at
Ddx42
AW763628
0.65
0.97
1430222_at
9130007G19Rik
BB538672
0.55
0.63

1447851_x_at
Atp10a
BB487289
0.65
0.89
1445536_at
AW061096
AW061096
0.55
0.86

1421986_at
Eif4e2
AI449084
0.65
0.87
1449915_at
Zfp202
NM_030713
0.55
0.79

1429295_s_at
Trip13
AK010336
0.65
0.58
1456784_at
″OTTMUSG




1420192_at
D16Bwg1494e
N28171
0.65
0.83
00000015762″
BB011034
0.55
0.73


1424451_at
Acaa1b
BC019882
0.64
0.53
1444304_at
A330102K23Rik
BE980520
0.54
0.89

1443918_at
2700050L05Rik
BG068962
0.64
0.86
1438679_at
Trim8
AI843272
0.54
0.70

1426313_at
Bre
BE984258
0.64
0.49
1456515_s_at
Tcfl5
AV044715
0.54
0.96

1439391_at
2810002I04Rik
BB559552
0.64
0.78
1455255_at
4833420G11Rik
AV250006
0.54
1.12

1446769_at
2810439F02Rik
AV028075
0.64
1.01
1430582_at
Shprh
BB496854
0.54
0.76

1438979_s_at
1700029I15Rik
AV043667
0.64
1.20
1452473_at
Prr15
AJ132433
0.53
0.60

1451434_s_at
Gpatch8
BC019948
0.64
0.88
1447580_at

AI835197
0.53
1.41

1420130_s_at
D10Wsu52e
AA409545
0.64
0.86
1427148_at
Pja2
BF160731
0.52
0.75

1445221_at

BB451452
0.64
0.96
1418019_at
Cpd
NM_007754
0.51
0.25

1441380_at
2810439F02Rik
BB044461
0.63
0.81
1454429_at
5830462O15Rik
AK018030
0.51
0.56

1455805_x_at
Ccdc22
BB165451
0.63
0.82
1456799_at
E130110O22Rik
BB540910
0.51
0.85

1456674_at
E130016E03Rik
BB772877
0.63
0.77
1417479_at
Ppp2r3c
AV367559
0.51
0.82

1429698_at
LOC208595
AK016498
0.63
0.82
1427963_s_at
Rdh9
BE979765
0.50
0.69

1448818_at
Wnt5a
BC018425
0.63
1.04
1420889_at
Hccs
BB129992
0.49
1.12

1429819_at
Nmnat1
AK017631
0.63
1.06
1459766_x_at
Sf1
BB055869
0.47
0.93

1451602_at
Snx6
BC025911
0.63
0.81
1431216_s_at
Dnajc6
BI730538
0.41
0.79

1421141_a_at
Foxp1
BG962849
0.63
1.05
1447773_x_at
6330409D20Rik
BB308792
0.41
1.22

1428665_at
Pfn4
BQ174081
0.63
0.73






1455075_at
Pigv
BB321057
0.62
1.10






1432589_at
Plcg1
BF166706
0.62
0.83






1447870_x_at
1110002E22Rik
BB099116
0.62
0.61



Probe = Affymetrix probe identification;

Symbol = official gene symbol;

FCH D1 = fold change in striatonigral (D1) cells;

FCH D2 = fold change in striatopallidal (D2) cells.
















































































































































































































































































































































































































































































































































































































Statistically over-represented Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms associated with genes whose expression was changed upon acute or chronic cocaine administration were searched (tables below). Amongst the most GO biological processes altered in D l-expressing striatonigral neurons upon chronic cocaine administration was the gamma-aminobutyric acid (GABA) signaling pathway (Gabrb3, Gabra1, Cacnb4, and Gabra4). This finding is relevant in light of positron emission tomography (PET) studies that have documented an enhanced sensitivity to the benzodiazepine lorazepam (binds GABA receptors) among chronic cocaine abusers (N. D. Volkow et al., Am J Psychiatry 155, 200-6 (February, 1998)).





TABLE 17



Top 1,000 striatonigral (D1) cell translated mRNAs enriched when compared to a reference sample










ProbeSymbol
FEN
Genbank
GeneSymbol
ProbeSymbol
FEN
Genbank
GeneSymbol












1452217_at
112.5
BE570050
Ahnak
1435749_at
19.85
AW911807
Gda

1426814_at
100.6
BM248309
AU024582
1423530_at
19.32
BB320288
Stk32c

1418357_at
71.38
NM_008241
Foxg1
1417356_at
19.31
AB003040
Peg3

1419231_s_at
70.83
NM_010661
Krt1-12
1425870_a_at
19.26
AF439339
Kcnip2

1427017_at
70.71
BB104560
Satb2
1427358_a_at
18.94
BC026671
Dapk1

1435564_at
68.33
BB547893
C230078M08Rik
1435615_at
18.72
BB277790
Zfp365

1457132_at
57.88
BF456117

1455034_at
18.64
BB703394
Nr4a2

1458499_at
47.5
AW123977
Pde10a
1444681_at
18.62
BB749686
D14Ertd171e

1443287_at
47.05
BB555669
Gm1337
1434248_at
18.36
BM243756
Prkch

1422079_at
45.93
NM_008856
Prkch
1427343_at
18.15
BC026377
Rasd2

1438022_at
45.81
BQ266518
Rab11fip3
1450071_at
18.15
BG694892
Ash1l

1457424_at
44.35
BB760085
Eya1
1451280_at
17.94
BB159263
MGI: 107562

1426815_s_at
44.33
BM248309
AU024582
1436329_at
17.56
AV346607
Egr3

1436268_at
40.01
AI841578
Ddn
1434653_at
17.09
AV026976
Ptk2b

1456051_at
38.14
BB282271
Drd1a
1434115_at
16.95
BQ176681


1416711_at
36.16
NM_009322
Tbr1
1424507_at
16.92
BC011277
Rin1

1420437_at
34.39
NM_008324
Indo
1452176_at
16.72
BB292874
Nup153

1455629_at
33.34
BE957273
Drd1a
1453129_a_at
16.55
AK004813
Rgs12

1454867_at
32.24
BB234631
Mn1
1427683_at
16.48
X06746
Egr2

1434917_at
31.38
BQ173923
Cobl
1418782_at
16.46
NM_009107
Rxrg

1428642_at
31.19
AK018094
Slc35d3
1452966_at
16.43
AK020296
9130430L19Rik

1423117_at
30.07
BB837171
Pum1
1457341_at
16.07
BE991676


1460325_at
29.16
BB837171
Pum1
1425503_at
16.07
AB037596
Gcnt2

1416776_at
24.48
NM_016669
Crym
1423171_at
15.52
BE947345
Gpr88

1424606_at
23.37
BC024854
Cplx3
1430306_a_at
15.47
AK004157
Atp6v1c2

1450339_a_at
22.89
NM_021399
Bcl11b
1456283_at
15.47
AV346211
Neto1

1449425_at
22.68
BCO26373
Wnt2
1427682_a_at
15.33
X06746
Egr2

1423802_at
22.57
BC017634
Camkv
1422034_a_at
15.24
NM_023128
Palm

1437698_at
22.46
AV370579
Myo5b
1449420_at
15.22
NM_008800
Pde1b

1427807_at
22.23
BC017159
4930448N21Rik
1450072_at
14.84
BG694892
Ash1l

1422720_at
21.52
BQ176915
Isl1
1435227_at
14.76
BM117007


1435083_at
21.47
BI155559
Ctxn1
1455200_at
14.73
BB818370
Pak6

1439854_at
21.22
BQ175572
AI838259
1433566_at
14.65
BB381618
Rasl10b

1450042_at
21.2
BB322201
Arx
1456054_a_at
14.47
BB314559
Pum1

1425756_at
21.04
AF425643
Rab40b
1435366_at
14.24
BB486367
D430042O09Rik

1436650_at
20.92
AV241894
Filip1
1426980_s_at
14.23
BC006054
E130012A19Rik

1436275_at
20.82
AW490636
Kcnip2
1424248_at
14.1
BB159263
MGI: 107562

1427975_at
20.66
AK008807
2210403B10Rik
1416953_at
13.95
NM_010217
Ctgf

1436566_at
20.43
AV364488
Rab40b
1433701_at
13.94
BB371430
Mpped1

1448364_at
13.87
U95826
Ccng2
1435296_at
10.95
AV349563
Adra2c

1418619_at
13.83
NM_008319
Icam5
1434521_at
10.89
BB148972
Rfxdc2

1450750_a_at
13.58
NM_013613
Nr4a2
1438665_at
10.88
BF456582
Smpd3

1450061_at
13.55
BM120053
Enc1
1444978_at
10.86
BM223267


1454043_a_at
13.52
AK015412
Kcnab1
1451331_at
10.75
BC026568
Ppp1r1b

1450723_at
13.4
BQ176915
Isl1
1422710_a_at
10.73
NM_021415
Cacna1h

1435748_at
13.37
AW911807
Gda
1430013_at
10.7
BE993118
2210008F06Rik

1425479_at
13.34
BF160651
Smyd5
1422902_s_at
10.63
NM_023799
Mgea5

1452202_at
13.28
BG069616
Pde2a
1422779_at
10.57
NM_021491
Smpd3

1432646_a_at
13.21
BE859789
2900097C17Rik
1428781_at
10.54
BI452905
1110014F24Rik

1452974_at
13.21
AK017551
Nol8
1460136_at
10.52
AI462839


1460038_at
13.11
BG065255
Pou3f1
1416691_at
10.51
NM_019581
Gtpbp2

1425130_a_at
13.06
U28216
Ptpn5
1450143_at
10.51
BB354696
Rasgrp1

1458342_at
13.01
BB313069

1435741_at
10.46
BB312125
Pde8b

1455190_at
12.97
BM114283
Gng7
1420666_at
10.35
BM117900
Doc2b

1436066_at
12.96
BI965477
Kalrn
1437125_at
10.24
BB476448
Camlaa

1428951_at
12.89
AK017551
Nol8
1454768_at
10.24
AV337635
Kcnf1

1436408_at
12.84
BE946298

1420978_at
10.23
NM_010938
Nrf1

1454906_at
12.83
BB266455
Rarb
1450227_at
10.22
BM199504
Ankrd6

1417560_at
12.81
BB478992
Sfxn1
1447359_at
10.22
AI326876
LOC381955

1440204_at
12.8
AW494150
3110039M20Rik
1436092_at
10.21
BB336256


1424037_at
12.77
BC027291
Itpka
1448327_at
10.11
NM_033268
Actn2

1422230_s_at
12.67
NM_007812
Cyp2a4; Cyp2a5
1420545_a_at
10.1
NM_029716
Chn1

1429422_at
12.57
AK016788
4933412E12Rik
1418691_at
10.03
NM_011268
Rgs9

1436532_at
12.51
BB326709
Dcamkl3
1423756_s_at
10.02
BC019836
Igibp4

1431936_a_at
12.33
AK009828
Neu2
1460343_at
10.02
AF401228
Neurl

1452298_a_at
12.18
AW546331
Myo5b
1421028_a_at
10.01
AI595932
Mef2c

1422153_a_at
12.11
NM_026853
Asb11
1429089_s_at
9.93
BG063749
2900026A02Rik

1427931_s_at
12.09
BG063905
Pdxk
1418015_at
9.916
BI689507
Pum2

1433627_at
12.04
AW546839
Sec23ip
1428950_s_at
9.758
AK017551
Nol8

1417279_at
11.94
NM_010585
Itpr1
1419033_at
9.756
AW556821
2610018G03Rik

1428139_at
11.9
AK015988
4930538D17Rik
1452879_at
9.72
AI848603
Synpo2

1450213_at
11.85
NM_013875
Pde7b
1441368_at
9.671
BB102769


1439552_at
11.83
BE985618
Trio
1447326_s_at
9.625
AW122925
Zfp261

1449634_a_at
11.74
AW046296
C030032C09Rik
1455564_at
9.621
BQ176236
Bcr

1438355_at
11.68
AI414870

1455620_at
9.572
BQ177219


1417355_at
11.64
AB003040
Peg3
1452507_at
9.568
AF022078
Dlx6

1456640_at
11.46
AW910872
Sh3rf2
1415904_at
9.554
BC003305
Lpl

1454721_at
11.44
AV257687
1110018G07Rik
1451507_at
9.554
BB280300
Mef2c

1435780_at
11.44
BG966595
Psd
1428338_at
9.553
AK019166
2610039E05Rik

1418047_at
11.42
NM_009717
Neurod6
1421353_at
9.538
NM_013875
Pde7b

1421176_at
11.39
BB354696
Rasgrp1
1419470_at
9.495
B1713933
Gnb4

1450026_a_at
11.37
AV306734
B3gnt1
1417114_at
9.466
AF163665
Gdl

1457311_at
11.36
AW490258
Camlaa
1448990_a_at
9.433
AI255256
Myo1b

1451604_a_at
11.23
BC014291
Acyrl1
1458406_at
9.404
BG144063


1416437_a_at
11.21
AF262046
Mapk8ip3
1430521_s_at
9.38
AW548480
Cpne8

1451726_at
11.18
BC020019
Mtmr6
1434025_at
9.365
BG069607


1440534_at
11.16
BB177862

1422621_at
9.363
BM507707
Ranbp2

1451808_at
11.16
U11075
Kcnj4
1423202_a_at
9.355
U22016
Ncor1

1434295_at
11.16
BE691356
Rasgrp1
1422165_at
9.336
X66603
Pou3f4

1457143_at
11.12
BB322292
Gli3
1433815_at
9.334
AV290082
MGI: 1923321

1416456_a_at
11.11
BC011134
Chia
1423489_at
9.324
BC021914
Mmd

1442166_at
11.08
BB273427
Cpne5
1460567_at
9.318
BB148972
Rfxdc2

1448366_at
11.05
NM_016801
Stx1a
1446492_at
9.312
BB396896
C630028F04Rik

1421739_a_at
11.05
NM_010768
Matk
1420580_at
9.273
NM_026249
4930429B21Rik

1427045_at
11.03
AI849322
Synpo
1417804_at
9.21
NM_011242
LOC381240

1457052_at
9.179
BG064867
AW536275
1427344_s_at
8.237
BC026377
Rasd2

1460555_at
9.176
BM242294
6330500D04Rik
1437929_at
8.236
AV306847
Dact2

1417680_at
9.153
NM_008419
Kcna5
1421817_at
8.23
AK019177
Gsr

1454656_at
9.123
AV271736
Spata13
1443612_at
8.216
AW123199


1458407_s_at
9.093
BG144063

1428852_at
8.213
AK018140
Dock3

1454752_at
9.081
AV307961
AI606861
1423561_at
8.172
AI838010
Nell2

1452368_at
9.043
AI853148
Bcr
1417440_at
8.166
NM_033566
Arid1a

1453273_at
9.038
AV142265
Kcnv1
1440849_at
8.044
AV327187
6330417G04Rik

1455701_at
9.019
BI965517
Snx26
1437918_at
8.028
AV374644
4930539E08Rik

1452476_at
8.997
W41214
Cacnb2
1433583_at
8.025
AV327248
Zfp365

1418169_at
8.988
BB223737
Zcchc14
1429285_at
8.014
AK009343
Serpina9

1423285_at
8.971
BB731671
Coch
1436734_at
8.003
BB523550
E130309F12Rik

1455447_at
8.93
BM116882
D430019H16Rik
1438985_x_at
8
BB158937
Otud5

1436998_at
8.924
BB428991
Ankrd43
1418017_at
7.974
BI689507
Pum2

1455220_at
8.9
BB353860
Frat2
1435435_at
7.965
BB357580
Cttnbp2

1419224_at
8.894
NM_033567
Cecr6
1455885_at
7.965
AV238106
6530401C20Rik

1442234_at
8.872
BB770422
Chst2
1423544_at
7.964
BB188812
Ptpn5

1439557_s_at
8.849
BB097063
Ldb2
1421446_at
7.962
NM_011102
Prkcc

1457729_at
8.819
AW492805

1454742_at
7.942
BB003229
Rasgef1b

1448978_at
8.804
NM_019867
Ngef
1419389_at
7.941
BQ180352
Pde10a

1419221_a_at
8.803
NM_016758
Rgs14
1426518_at
7.939
AV333951
Tubgcp5

1456515_s_at
8.774
AV044715
Tcfl5
1423636_at
7.931
AK017259
Wdr31

1455272_at
8.747
BB429139
Grm5
1433767_at
7.931
AV257687
1110018G07Rik

1418373_at
8.673
NM_018870
Pgam2
1416562_at
7.91
AF326547
Gad1

1422787_at
8.672
NM_019873
Fkbpl
1427450_x_at
7.907
BI080370
Myo1b

1437091_at
8.631
AV323885
Accn4
1454845_x_at
7.905
AW049955
Gpr24

1451517_at
8.627
AF420001
Rhobtb2
1420388_at
7.905
NM_008939
Prss12

1417462_at
8.62
NM_007598
Cap1
1422901_at
7.896
NM_023799
Mgea5

1455361_at
8.612
AW493391
Dgkb
1416804_at
7.845
NM_053252
LOC114601

1416783_at
8.595
NM_009311
Tac1
1416350_at
7.826
NM_078477
Klf16

1428872_at
8.573
AW495537
4121402D02Rik
1440148_at
7.825
AI852874
Gpr6

1418881_at
8.568
NM_054095
Efcbp2
1424796_at
7.809
BC024705
1700054N08Rik

1431164_at
8.563
AK017818
Rragd
1457440_at
7.808
BB451927
Sstr4

1455754_at
8.54
BQ043839
Lmo3
1427618_at
7.801
BQ176417
Cdh9

1439767_at
8.526
BB130159
Dlgap2
1447863_s_at
7.794
BB322941
Nr4a2

1432378_at
8.526
AK021043
C030004G16Rik
1428535_at
7.775
AK004276
9430020K01Rik

1422018_at
8.509
NM_010437
Hivep2
1431394_a_at
7.754
AK014938
Lrrk2

1441894_s_at
8.487
BB071890
Grasp
1430675_at
7.733
BM932606
2900055J20Rik

1421142_s_at
8.473
BG962849
Foxp1
1460206_at
7.728
NM_019518
Grasp

1423551_at
8.43
BB776961
Cdh13
1423186_at
7.703
BM228957
Tiam2

1420961_a_at
8.428
NM_028582
Ivns1abp
1448807_at
7.7
NM_133849
Hrh3

1455683_a_at
8.423
BB451404
Tbc1d8
1456401_at
7.693
BB078175
Cacnb2

1434077_at
8.407
AV222037
Wdr37
1450690_at
7.69
BM507707
Ranbp2

1436312_at
8.405
AV317621
Zfpn1a1
1434429_at
7.684
AV348245
Syt16

1445539_at
8.395
BE687857
Pde7b
1451506_at
7.667
BB280300
Mef2c

1435221_at
8.388
BM220880
Foxp1
1427426_at
7.619
AV362204
Kcnq5

1441733_s_at
8.387
C88147
Nup153
1445691_at
7.613
AW121426
Chn1

1426915_at
8.387
BC021490
Dapk1
1434695_at
7.594
AV270035
Dtl

1416286_at
8.354
NM_009062
Rgs4
1425833_a_at
7.582
AF326551
Hpca

1418714_at
8.352
NM_008748
Dusp8
1456336_at
7.561
BB182912
A330102K23Rik

1427005_at
8.339
BM234765
Plk2
1437865_at
7.55
AW546433
Spata13

1453782_at
8.33
BI737125
3021401C12Rik
1435605_at
7.538
BB125424
Actr3b

1421101_a_at
8.319
NM_010698
Ldb2
1422678_at
7.531
AK002443
Dgat2

1416266_at
8.283
AF026537
Pdyn
1449472_at
7.521
NM_008151
Gpr12

1423376_a_at
8.251
AV341904
Dok4
1456786_at
7.493
BB097063
Ldb2

1429617_at
8.246
BM119209
Cyld
1429741_at
7.479
AV142265
Kcnv1

1442434_at
7.465
BM195829
D8Ertd82e
1441963_at
6.895
BB427703
MGI: 2656976

1442113_at
7.463
BB183350
5330417C22Rik
1448989_a_at
6.888
AI255256
Myo1b

1448656_at
7.462
NM_007581
Cacnb3
1421027_a_at
6.878
AI595932
Mef2c

1423012_at
7.457
NM_018801
Syt7
1455296_at
6.854
BE952286
Adcy5

1446498_at
7.409
BB551879
E230031K19
1435666_at
6.846
AW553439


1422068_at
7.389
NM_011141
Pou3f1
1435071_at
6.829
AV327165
Zfyve1

1417129_a_at
7.369
U68384
Mrg1
1439548_at
6.82
BB390705
Rap2b

1423344_at
7.368
AK010968
Epor
1429104_at
6.817
AK012581
0610025L06Rik

1454877_at
7.333
BQ174721
Sertad4
1437460_x_at
6.805
BB264363
Rin1

1426585_s_at
7.326
BM209765
Mapk1
1455758_at
6.804
BM215011


1426901_s_at
7.32
BB657856
Camta2
1425929_a_at
6.804
AF249668
Rnf14

1423240_at
7.31
BG868120
Src
1457919_at
6.801
BB446049
D030054H15Rik

1420981_a_at
7.304
NM_010723
Lmo4
1443694_at
6.8
BB794177
Rgs20

1431074_a_at
7.298
BI654600
Pitpnc1
1417090_at
6.799
NM_009037
Rcn1

1438889_at
7.294
BB183456

1452964_at
6.774
AK016577
4932702F08Rik

1418847_at
7.268
NM_009705
Arg2
1431230_a_at
6.748
Z74630
Btbd9

1436733_at
7.257
BB523550
E130309F12Rik
1439569_at
6.734
AV291031
Gpr83

1424201_a_at
7.248
AW537349
Seh1l
1439866_at
6.733
BQ173950
Parc

1457979_at
7.244
BM938335

1447728_x_at
6.677
BB718260
Hspa9a

1421141_a_at
7.232
BG962849
Foxp1
1435210_s_at
6.675
BQ174936
Snph

1450655_at
7.231
AA214868
Herd1
1419542_at
6.653
NM_010021
Dazl

1437586_at
7.22
BB756908
Cnot4
1430617_at
6.632
BB238604
Oip5

1434008_at
7.201
BE993937
Scn4b
1419137_at
6.631
NM_021423
Shank3

1419696_at
7.198
NM_013488
Cd4
1420530_at
6.63
AW553317
Neud4

1417341_a_at
7.194
NM_025800
Ppp1r2
1447301_at
6.625
BG228875
Gm258

1434458_at
7.182
BB444134
Fst
1418144_a_at
6.621
NM_008847
Pip5k1b

1438784_at
7.162
BB329234

1422683_at
6.62
NM_022986
Irak1bp1

1421789_s_at
7.149
NM_007478
Arf3
1455056_at
6.612
BM231903
Lmo7

1452624_at
7.147
BM900077
Lrrtm1
1421346_a_at
6.608
NM_009320
Slc6a6

1450835_a_at
7.147
NM_020014
Gfra4
1434359_at
6.607
AV329070
6330500D04Rik

1419390_at
7.143
BQ180352
Pde10a
1423640_at
6.584
BC026512
Synpr

1429316_at
7.138
AK018120
Rasgef1a
1418080_at
6.581
AW125856
B4galt2

1436381_at
7.133
BQ175774
Dlgap3
1421140_a_at
6.58
BG962849
Foxp1

1433969_at
7.121
AU067824
AU067824
1451285_at
6.58
AF224264
Fus

1426009_a_at
7.119
BC003763
Pip5k1b
1451236_at
6.57
BC026463
Rerg

1434090_at
7.098
BB037094
4930429A22Rik
1435043_at
6.569
BB794831
Plcb1

1437018_at
7.083
BG072348
Pnma2
1434029_at
6.567
AW545676
LOC545794

1430127_a_at
7.067
AK007904
Ccnd2
1443888_at
6.535
BB426608


1415743_at
7.065
NM_010412
Hdac5
1430037_at
6.531
AK017836
Snx27

1452940_x_at
7.049
AI595920
Pitpnc1
1447178_at
6.529
BB173057


1456280_at
7.049
BG067086
Clspn
1420857_at
6.525
NM_133737
Lancl2

1416398_at
7.036
BB474887
Mesdc1
1460447_at
6.52
AK019372
3000003F02Rik

1423231_at
7.034
AK002933
Nrgn
1420146_at
6.514
AI429207
Tiam2

1451270_at
7.031
BC020036
Dusp18
1419216_at
6.506
NM_009734
Azi1

1452467_at
7.029
BQ042988
Mmab
1460262_a_at
6.492
NM_026091
1700037H04Rik

1438799_at
7.028
AV338180
Dlx6os1
1421340_at
6.483
NM_008580
Map3k5

1429637_at
7.024
AK008987
2210419I08Rik
1415976_a_at
6.468
AU080787
Carhsp1

1432073_at
7.021
AK007198
1700113I22Rik
1419066_at
6.46
NM_030244
Ier5l

1435558_at
7.019
BB351248
Bai2
1422706_at
6.45
AV370981
Tmepai

1436738_at
6.98
AV094878
AI449441
1459679_s_at
6.45
AA406997
Myo1b

1456785_at
6.96
BQ175889
Crsp2
1438751_at
6.44
BB736474
Slc30a10

1435808_at
6.954
BB174377
A230051G13Rik
1454960_at
6.43
BI646741
Smad3

1439855_at
6.931
AV341449
BC023818
1425510_at
6.43
BM213279
Mark1

1417524_at
6.918
NM_009920
Cnih2
1429224_at
6.419
AK017476
Pnma1

1435553_at
6.896
AV376136
Pdzk3
1449468_at
6.412
NM_012028
St6galnac5

1431920_a_at
6.896
AK008082
2010004A03Rik
1450748_at
6.41
NM_021491
Smpd3

1431422_a_at
6.399
AK009744
Dusp14
1418535_at
6.023
NM_016846
Rgl1

1423532_at
6.374
AI850285
Rnf44
1425249_a_at
6.007
AB000828
Tyro3

1422335_at
6.373
NM_007418
Adra2c
1453886_a_at
6.005
AK017037
Slc25a26

1422527_at
6.373
NM_010386
H2-DMa
1421897_at
6
AI385733
Elk1

1421191_s_at
6.372
NM_053187
Gopc
1455178_at
6
BB496468
Rutbc1

1434762_at
6.358
BF457736
A730041O15Rik
1436403_at
5.998
BB452429
BC025575

1420377_at
6.336
BG071333
St8sia2
1420610_at
5.979
AV024339
Prkacb

1437001_at
6.333
BQ173949
Gsk3b
1416996_at
5.979
BC005421
Tbe1d8

1435763_at
6.322
BI218684
Tbe1d16
1433451_at
5.967
BB177836
Cdk5r1

1434153_at
6.316
BI408715
Shb
1438664_at
5.966
BB216074
Prkar2b

1427565_a_at
6.309
AF213387
Abcc5
1417461_at
5.957
NM_007598
Cap1

1427468_at
6.307
M81483
Ppp3cb
1459051_at
5.956
BB781615
6530418L21Rik

1440940_at
6.305
BE949510
Cacnb1
1426450_at
5.955
BM207017
Plel2

1449956_at
6.301
NM_011104
Prkce
1455301_at
5.95
BG064092
BQ952480

1436100_at
6.298
AV347995
Sh2d5
1423973_a_at
5.949
BC024935
Arf3

1420871_at
6.293
BF472806
Gucy1b3
1417812_a_at
5.948
NM_008484
Lamb3

1455225_at
6.293
AV237615
Syne1
1423694_at
5.934
BC006935
Ketd10

1448709_at
6.292
NM_033566
Arid1a
1417612_at
5.933
BF147705
Ier5

1438224_at
6.287
BE572265
Zswim5
1425680_a_at
5.92
AF110396
Btrc

1448023_at
6.278
BB662566
Kalrn
1448063_at
5.918
AW226526
Iqsec2

1426024_a_at
6.278
AF187147
Dbn1
1448414_at
5.911
NM_011232
Rad1

1435500_at
6.266
BB270778
Rab26
1423478_at
5.909
BF660388
Prkeb1

1451751_at
6.258
AF335325
Ddit4l
1449921_s_at
5.903
NM_009947
Cpne6

1453836_a_at
6.25
AK006949
Mgll
1429438_at
5.888
AV318805
Bcor

1417559_at
6.244
BB478992
Sfxn1
1457077_at
5.875
BB306048


1456509_at
6.236
BB382807
1110032F04Rik
1427299_at
5.87
BE376079
Rps6ka3

1440361_at
6.23
BB272510

1428892_at
5.869
AK004331
Ppil1

1425465_a_at
6.225
AV317107
Senp2
1449164_at
5.866
BC021637
Cd68

1460279_a_at
6.222
AF043219
Gtf2i
1434763_at
5.864
BF457736
A730041O15Rik

1440207_at
6.219
BI155142
AI505034
1435265_at
5.861
BF466929


1444139_at
6.189
BG797099
Ddit4l
1436754_at
5.86
BB360523
AI839735

1421860_at
6.171
BG065300
Clstn1
1433642_at
5.857
BB384173
Arfrp2

1458443_at
6.17
BE986758
Mect1
1434154_at
5.83
BQ177107
Kctd13

1448374_at
6.167
AK005130
Med28
1449158_at
5.824
NM_010607
Kcnk2

1454762_at
6.164
BM932447
Xkrx
1441867_x_at
5.82
AI480494
4930534B04Rik

1451908_a_at
6.154
BC005766
Sec14l1
1429228_at
5.818
BE980134
4930534B04Rik

1429718_at
6.15
BB782729
Slitrk5
1421116_a_at
5.811
NM_024226
Rtn4

1451696_at
6.146
BC004695
Zfp64
1426416_a_at
5.809
AV216410
Yipf4

1449957_at
6.14
NM_007955
Ptprv
1449241_at
5.803
NM_053105
Klhl1

1439041_at
6.128
BM239325
Slc39a10
1453093_at
5.801
AK018593
Rasgef1c

1433471_at
6.127
AI323642
Tcf7
1417305_at
5.798
NM_007463
Apeg1

1430640_a_at
6.126
BI695530
Prkar2b
1441065_at
5.787
BB305753


1429111_at
6.102
AV270892
Tln2
1442754_at
5.778
BE692283
C030013G03Rik

1421768_a_at
6.092
NM_011982
Homer1
1440711_at
5.777
BB426320
C630001G18Rik

1421000_at
6.085
A1448404
Cnot4
1418263_at
5.774
BC024852
Ddx25

1460718_s_at
6.076
AF192558
Mtch1
1421175_at
5.771
NM_008666
Myt1l

1434775_at
6.06
AW543460
Pard3
1431056_a_at
5.77
AK017272
Lpl

1435218_at
6.058
BI134758
Rasgef1a
1457702_at
5.766
BB284435
Gpr12

1452861_at
6.049
AK008485
2010300C02Rik
1422407_s_at
5.761
NM_008284
Hras1

1454609_x_at
6.047
BB770958
6430527G18Rik
1422208_a_at
5.761
BC016135
Gnb5

1457651_x_at
6.046
BB270375
Rem2
1455387_at
5.76
AV112972
1110001M19Rik

1438068_at
6.042
BB251859

1455191_x_at
5.747
BB822856
Pip5k1b

1452380_at
6.026
BB075797
Epha7
1440918_at
5.735
AW495511
Rasgrf2

1428184_at
6.025
BB348639
3110035E14Rik
1418982_at
5.731
BC011118
Cebpa

1425466_at
6.025
AV317107
Senp2
1433988_s_at
5.729
BG075755
C230098O21Rik

1426012_a_at
6.024
BC010499
2610301G19Rik
1435392_at
5.726
BB277182
Wdr17

1448842_at
5.714
NM_033037
Cdo1
1431749_a_at
5.467
AK013548
Rasgrp1

1426339_at
5.713
AB060081
Ak5
1434902_at
5.456
BB163668
Rnf157

1452917_at
5.709
AK011489
Rfc5
1423488_at
5.453
BC021914
Mmd

1427063_at
5.709
AK017241
5330417C22Rik
1452718_at
5.452
BG073486
Edd1

1436005_at
5.689
BQ174136
Sfrs14
1448598_at
5.449
NM_011846
Mmp17

1447017_at
5.687
BE956696

1436142_at
5.446
BM218877
3526401B18Rik

1426108_s_at
5.685
AY094172
Cacnb1
1424047_at
5.427
BC016218
Dera

1451008_at
5.683
BB360510
St8sia3
1451483_s_at
5.421
BC024705
1700054N08Rik

1424899_at
5.68
BC005737
Nmnat3
1424132_at
5.42
BC011083
Hras1

1428527_at
5.679
AK011015
Snx7
1454784_at
5.419
AV340742
Hs3st2

1415985_at
5.679
NM_133953
Sf3b3
1454835_at
5.413
AV340515
Epm2aip1

1448468_a_at
5.676
AF033003
Kcnab1
1448724_at
5.409
NM_009895
Cish

1418592_at
5.675
NM_021422
Dnaja4
1451098_at
5.406
BC010524
Pcoln3

1416982_at
5.671
AI462296
Foxo1
1418784_at
5.39
NM_008168
Grik5

1433460_at
5.669
BB329157
Ttc7b
1449470_at
5.388
NM_010053
Dlx1

1420496_at
5.666
NM_021489
F12
1427082_at
5.383
AK014586
4632417N05Rik

1444661_at
5.663
BB247791
Gpr26
1455085_at
5.379
BI526033
1700086L19Rik

1448546_at
5.651
BB703307
Rassf3
1455033_at
5.363
BB325849
B430201A12Rik

1447707_s_at
5.649
BB367174
Pde2a
1426241_a_at
5.356
AB030906
Scmh1

1418743_a_at
5.647
NM_021344
MGI: 1930803
1456755_at
5.353
AI467545
2310001H13Rik

1415834_at
5.646
NM_026268
Dusp6
1420718_at
5.352
NM_011856
Odz2

1448747_at
5.636
AF441120
Fbxo32
1457632_s_at
5.35
BB207647
Mrg1

1417192_at
5.631
NM_138599
Tomm70a
1427579_at
5.348
BE653576
Rhbdl4

1416754_at
5.63
NM_008923
Prkar1b
1423228_at
5.347
BG066773
B4galt6

1425617_at
5.626
U91922
Dhx9
1451254_at
5.344
AF367244
Ikbkap

1417522_at
5.606
AF441120
Fbxo32
1416242_at
5.343
NM_026167
Klhl13

1416770_at
5.604
NM_021537
Stk25
1425679_a_at
5.34
AF109769
Mapk8ip1

1421498_a_at
5.595
NM_023450
2010204K13Rik
1439611_at
5.307
BE947522
Chrm1

1441363_at
5.594
BQ174855
L00546381
1425059_at
5.306
BC022899
Hrmt1l6

1421037_at
5.594
BG070037
Npas2
1454985_at
5.303
BI872009
D030051N19Rik

1452278_a_at
5.59
BG922448
Hace1
1435128_at
5.301
BB429313
Baiap2

1421048_a_at
5.583
NM_023249
Ypel1
1434800_at
5.301
BQ175909
Sv2b

1422977_at
5.577
NM_010327
Gp1bb
1421959_s_at
5.298
AF458089
Adcy3

1430168_at
5.57
AK020589
Cstad
1434819_at
5.297
BB709312
St6gal2

1418789_at
5.563
NM_133742
Sntg2
1448687_at
5.291
NM_026125
C1qdc2

1438213_at
5.563
BB128962
A830018L16Rik
1455279_at
5.284
BG070552
Gm1060

1420965_a_at
5.546
BM120053
Enc1
1454832_at
5.275
BG228702
Phactr1

1451630_at
5.544
BC018513
Ttl
1418181_at
5.273
AK014601
Ptp4a3

1426852_x_at
5.539
X96585
Nov
1420872_at
5.27
BF472806
Gucy1b3

1456946_at
5.531
BE949337
4831416G18Rik
1423658_at
5.267
BCO23131
Sppl3

1426354_at
5.53
AK009033
Bap1
1455242_at
5.267
BM220880
Foxp1

1422293_a_at
5.527
NM_134112
Kctd1
1456444_at
5.265
BB126849
Fbxo41

1433724_at
5.519
BM235412
D15Ertd621e
1456930_at
5.264
BE989461
Camsap1

1449125_at
5.518
NM_025566
Tnfaip8l1
1450520_at
5.261
NM_019430
Cacng3

1421622_a_at
5.518
NM_019688
Rapgef4
1438417_at
5.256
BB809239
Pwwp2

1436216_s_at
5.516
BM234799
2610204M08Rik
1434594_at
5.255
BB497449
Dnahc1

1422705_at
5.515
AV370981
Tmepai
1424175_at
5.249
BC017689
Tef

1444723_at
5.506
BB049759
6530418L21Rik
1424028_at
5.249
BCO23107
5830457O10Rik

1441350_at
5.505
AV302620
Fgf3
1435367_at
5.244
BQ177154
Mapk4

1435204_at
5.501
BG065273
Hrmt1l4
1437485_at
5.236
BB098161
Nos1ap

1422799_at
5.501
AK019427
Bat2
1452506_a_at
5.231
BC011427
Ilkap

1427067_at
5.498
AJ404329
4933439F18Rik
1448553_at
5.228
NM_080728
Myh7

1437580_s_at
5.492
C77054
Nek2
1424389_at
5.228
BCO26743
Nupl1

1452057_at
5.49
BG801851
Actr1b
1451273_x_at
5.223
BCO25546
BC025546

1437405_a_at
5.489
BB787243
Igfbp4
1441928_x_at
5.223
BB139475
Ell

1439618_at
5.476
AI448308
Pde10a
1431717_at
5.222
AK014386
3526401B18Rik

1450910_at
5.213
AV261931
Cap2
1424481_s_at
5.042
BC027106
MGC38735

1419581_at
5.212
AI646416
Dlgh4
1422751_at
5.039
NM_011599
Tle1

1423395_at
5.202
BM021706
Tsnax
1423699_at
5.036
BC003900
D15Ertd785e

1456818_at
5.201
BB279083
Stk32a
1433805_at
5.034
BQ032637
Jak1

1448554_s_at
5.199
NM_080728
Myh6
1416087_at
5.03
NM_007457
Ap1s1

1441369_at
5.193
BB329116

1419522_at
5.027
NM_026021
Zmynd19

1448805_at
5.192
NM_009480
Usf1
1449494_at
5.026
AY026947
Rab3c

1435844_at
5.19
BB164247
A330009N23Rik
1425132_at
5.026
AF448840
Neto1

1443129_at
5.19
BB363699

1436648_at
5.025
BB251623
Nanos1

1421312_a_at
5.19
NM_010630
Kifc2
1443676_at
5.023
AV339935
Slc4a4

1428676_at
5.187
AK004939
Tmprss6
1451475_at
5.023
BC019530
Plxnd1

1454759_at
5.183
AV230461
Git1
1421225_a_at
5.022
NM_018760
Slc4a4

1455080_at
5.182
BB375209
Ppp1r16b
1420999_at
5.022
A1448404
Cnot4

1454092_a_at
5.182
AK017176
Gtf2h3
1441967_at
5.021
BB467791
BC023835

1438663_at
5.18
BF730196
Bat2d
1439662_at
5.018
BM124609
Homer1

1416050_a_at
5.176
NM_016741
Scarb1
1437750_at
5.015
BI133257
2310037P21Rik

1435618_at
5.174
BB473446
Pnma2
1426897_at
5.011
AV122997
Rcc2

1427523_at
5.168
AI509029
Six3
1424759_at
5.006
BC017528
Arrdc4

1450930_at
5.167
AK002992
Hpca
1417393_a_at
4.997
NM_026125
C1qdc2

1433987_at
5.163
AV153557
Hpcal4
1435588_at
4.996
BQ031098


1419742_at
5.16
NM_026091
1700037H04Rik
1450486_a_at
4.991
X91813
Oprl1

1440371_at
5.151
BQ176223

1455108_at
4.989
BM119888
Eif4e2

1434825_at
5.151
BQ175287
LOC381742
1459920_at
4.988
AA163908
4933415E08Rik

1423420_at
5.148
AK018378
Adrb1
1417216_at
4.986
NM_138606
Pim2;

1434415_at
5.141
BB797871




LOC229005

1417716_at
5.139
U82470
Got2
1457696_at
4.982
AI835553
Rilp

1449188_at
5.139
NM_021565
Midn
1452938_at
4.979
BQ174247
C030032C09Rik

1417032_at
5.138
AF296657
Ube2g2
1433450_at
4.978
BB177836
Cdk5r1

1441815_at
5.136
AI851453
AI851453
1418172_at
4.974
AF117613
Hebp1

1435767_at
5.132
BE951842
Scn3b
1422552_at
4.965
NM_023396
Rprm

1426430_at
5.131
AV264681
Jag2
1422255_at
4.964
NM_021275
Kcna4

1438913_x_at
5.127
AW322533
Hdgfrp2
1423442_a_at
4.962
BE854125
Fbxw2

1449007_at
5.127
NM_009770
Btg3
1460285_at
4.947
NM_133721
Itga9

1434805_at
5.127
BG063049
Mllt1
1452911_at
4.943
AK017680
Spred1

1423049_a_at
5.126
AK002271
Tpm1
1452851_at
4.937
AK019578
Tnrc4

1423352_at
5.123
BE693025
Crispld1
1422609_at
4.936
BE648432
Arpp19

1424475_at
5.121
BI157430
Camkk2
1429174_at
4.936
AK014304
Wdr34

1452783_at
5.118
AK005500
Fndc3b
1448922_at
4.934
NM_024438
Dusp19

1425363_at
5.113
BC022180
Galgt1
1425711_a_at
4.932
M94335
Akt1

1455313_at
5.104
BB336053
Ablim2
1454844_at
4.929
AW049955
Gpr24

1455703_at
5.102
BG074736
Akt2
1416155_at
4.928
NM_008253
Hmgb3

1434832_at
5.099
BB364488
Foxo3a
1423311_s_at
4.927
BQ177165
Tpbg

1439888_at
5.096
BB271581

1418497_at
4.922
AF020737
Fgf13

1433747_at
5.093
BQ176475
Lnpep
1418463_at
4.915
NM_008841
Pik3r2

1435206_at
5.092
BB767169
Slc24a4
1416448_at
4.914
NM_025922
Itpa

1428173_at
5.088
AK005562
Eml2
1428357_at
4.912
AK011462
2610019F03Rik

1416586_at
5.084
NM_008616
Zfp239
1450269_a_at
4.907
NM_008826
Pfkl

1442370_at
5.082
BB267006

1424199_at
4.905
AW537349
Seh1l

1417311_at
5.075
NM_024223
Crip2
1433833_at
4.902
BG064539
Fndc3b

1440166_x_at
5.066
BB378627
Htr1d
1426383_at
4.898
BF303057
Ciy2

1416402_at
5.066
AV382118
Abcb10
1417626_at
4.898
NM_031401
Usmg4

1422807_at
5.065
NM_007480
Arf5
1415998_at
4.891
NM_011694
Vdac1

1455771_at
5.062
BB467799
Bzrap1
1424208_at
4.891
BC011193
Ptger4

1418417_at
5.058
NM_010827
Msc
1454086_a_at
4.889
AK013416
Lmo2

1448411_at
5.055
NM_011716
Wfs1
1424503_at
4.886
BC006596
Rab22a

1435598_at
5.043
BE996371
Shc2
1456766_at
4.883
BB036966
C330001K17Rik

1423415_at
4.883
BB110067
Gpr83
1427912_at
4.699
AK003232
Cbr3

1434082_at
4.882
BM243464
Pctk2
1458351_s_at
4.697
BB428573
Klhl2

1433707_at
4.881
BB430205
Gabra4
1422762_at
4.697
AB008867
Kif17

1426710_at
4.878
BB396904
Calm3
1433962_at
4.693
BB131965
6720458F09Rik

1417897_at
4.878
NM_134155
Brms1
1458518_at
4.692
BM935843
Cpeb2

1437292_at
4.874
BB431052
A330019N05Rik
1426792_s_at
4.691
BC024790
Rusc2

1416605_at
4.874
BC024944
Nola2
1422718_at
4.688
NM_009682
Ap3s2

1425051_at
4.872
AK010892
Isoc1
1440258_at
4.685
BE951512
Kcnq2

1426539_at
4.872
AI117611
Usp11
1443225_at
4.684
BB396526
Acvr1c

1427006_at
4.87
BB339051
Rapgef1
1416409_at
4.68
AB034914
Acox1

1434563_at
4.87
BM207149
Rps6kc1
1416215_at
4.68
NM_016810
Gosr1

1452292_at
4.869
AV271093
Ap2b1
1417003_at
4.679
BC021536
0610012G03Rik

1421975_a_at
4.868
AF189769
Add2
1434904_at
4.678
AW321867
Hivep2

1455024_at
4.868
AV269836
Tlk1
1428434_at
4.677
AK012833
Zcchc12

1423519_at
4.855
BE457744
2210412D01Rik
1436225_at
4.676
BB082386
Trpm2

1442180_at
4.855
BB382040
Dleu7
1439867_at
4.676
BB079377
Ubox5

1435974_at
4.855
BB271482
Arhgef9
1429229_s_at
4.674
BE980134
4930534B04Rik

1428839_at
4.854
AK005105
Wdr53
1421046_a_at
4.673
NM_130881
Pabpc4;

1431751_a_at
4.85
AK012553
Mpped2



LOC432881

1426069_s_at
4.849
BC016100
Slc7a4
1453323_at
4.664
AK013806
2900079G21Rik

1448285_at
4.848
NM_009062
Rgs4
1429392_at
4.656
BI558553
Wdr40a

1452092_at
4.848
AK019474
4631426J05Rik
1428718_at
4.656
AW490544
Scrn1

1456144_at
4.842
AW537064
Nav3
1424225_at
4.649
AF398969
Asb8

1454797_at
4.838
AW556098
Tmem55b
1435942_at
4.647
BE993301
Kcnq2

1426465_at
4.838
BG066219
Dlgap4
1447711_x_at
4.646
BB265147
4933412E12Rik

1427954_at
4.837
BC028785
BC048403
1439497_at
4.637
BG065013
4933415E08Rik

1451914_a_at
4.834
AF100423
Add2
1453734_at
4.633
BF165715
Atrx

1442562_at
4.83
BB346556
D630040G17Rik
1419056_at
4.632
AF038538
Rtn2

1450710_at
4.825
NM_021878
Jarid2
1447124_at
4.631
BE634581


1449147_at
4.821
NM_023850
Chst1
1447861_x_at
4.629
AV329643
Mrg1

1435285_at
4.816
BB731805
Mpped2
1425844_a_at
4.622
AF034568
Rngtt

1425031_at
4.81
AB077383
Fcmd
1449063_at
4.614
BC009024
Sec22l1

1433714_at
4.808
AV338618
Sult4a1
1451722_s_at
4.611
BF160651
Smyd5

1421606_a_at
4.794
NM_013873
Sult4a1
1417349_at
4.611
NM_019788
Pldn

1450008_a_at
4.783
NM_007614
Ctnnb1
1429367_at
4.611
BB826276
2510001I10Rik

1421107_at
4.778
NM_021420
Stk4
1420901_a_at
4.609
NM_010438
Hk1

1437579_at
4.773
C77054
Nek2
1437017_at
4.606
BB046727
AI480653

1434058_at
4.772
BB667459
Mtmrl2
1420985_at
4.606
BG694892
Ash1l

1432295_a_at
4.77
AK009634
2310035K24Rik
1428878_a_at
4.602
AI595920
Pitpnc1

1419737_a_at
4.769
NM_010699
Ldh1
1436387_at
4.601
BB398124
C330006P03Rik

1423200_at
4.765
U22016
Ncor1
1427232_at
4.601
AV291373
Sdccag33

1449770_x_at
4.753
N28171
D16Bwg1494e
1426978_at
4.6
AW682368
Klhl2

1416683_at
4.75
NM_138749
Plxnb2
1416550_at
4.6
BB320416
Slc35b4

1425718_a_at
4.747
BC004092
Ivns1abp
1428903_at
4.599
AK014134
3110037I16Rik

1422590_at
4.745
NM_007668
Cdk5
1440376_at
4.597
AI747062
Fbxo41

1438196_at
4.739
BB727537
Gpd1l
1434228_at
4.594
AV255921
Ppm2c

1417794_at
4.738
NM_019831
Zfp261
1460030_at
4.594
BB355811
Hecw1

1434693_at
4.737
BE952823

1450484_a_at
4.594
AK004595
Tyki

1442099_at
4.733
BM227490
Usp31
1455140_at
4.593
AV376568
A330068P14Rik

1442220_at
4.733
BB313387
Ube3a
1451998_at
4.592
BC024597
4930485D02Rik

1435547_at
4.728
BQ175722
Mkl2
1460116_s_at
4.59
AI450584
Spred1

1426051_a_at
4.725
BC006628
Cenpb
1427286_at
4.587
BB130195
D11Bwg0517e

1434476_at
4.713
BM119896
Mect1
1437861_s_at
4.584
BB335101
Prkce

1440108_at
4.711
BM964154
Foxp2
1445605_s_at
4.581
AI835491
4921533L14Rik

1423330_at
4.708
AK006149
Ensa
1415803_at
4.578
AF010586
Cx3cl1

1429443_at
4.706
AK014396
Cpne4
1449511_a_at
4.577
NM_133772
Ssbp4

1419420_at
4.577
NM_012028
St6galnac5
1416853_at
4.47
BC017126
Ncdn

1425656_a_at
4.575
AF390178
Baiap2
1426644_at
4.464
BC002196
Tbc1d20

1435222_at
4.575
BM220880
Foxp1
1434339_at
4.461
AW548221
Fnbp1l

1428864_at
4.575
AK017419
5530400B01Rik
1428327_at
4.46
BB010301
2310001H13Rik

1423831_at
4.575
BB756794
Prkag2
1451138_x_at
4.459
BC005748
2700087H15Rik

1439148_a_at
4.574
BE914497
Pfkl
1449863_a_at
4.459
NM_010056
Dlx5

1428540_at
4.566
BE944524
3321401G04Rik
1417178_at
4.458
NM_016867
Gipc2

1426742_at
4.562
AK019459
Atp5f1
1423269_a_at
4.456
BB663717
Nedd4l

1455026_at
4.56
BE947961
Sbno1
1416292_at
4.454
NM_007452
Prdx3

1452234_s_at
4.555
BF457957
D16Bwg1494e
1435698_at
4.452
BQ176694
4921505C17Rik

1425760_a_at
4.554
AF006467
Pitpnm1
1419838_s_at
4.45
AI385771
Plk4

1434682_at
4.554
AV347367
6430601A21Rik
1452063_at
4.448
AK010736
2410081M15Rik

1428095_a_at
4.552
AK004920
Tmem24
1423038_at
4.446
BQ174465
Stx6

1447696_x_at
4.552
BB091427
Adcy5
1424645_at
4.446
BB795102
Tnrc6c

1415710_at
4.55
BM123013
BC038311
1434102_at
4.441
BI687652
Nfib

1455482_at
4.54
BQ175609
Ap2a2
1422431_at
4.439
NM_053201
Magee1

1435425_at
4.539
AV212090
4933439C20Rik;
1459994_x_at
4.435
AV027486
Trfr2




Pisd
1418467_at
4.433
NM_025891
Smarcd3

1438841_s_at
4.539
AV002218
Arg2
1428941_at
4.429
AK017929
Zfp198

1423785_at
4.538
BE995700
Egln1
1439422_a_at
4.429
AV048291
C1qdc2

1453000_at
4.538
AK005444
Camsap1l1
1424480_s_at
4.428
BC026151
Akt2

1429623_at
4.534
AV261187
Zfp644
1419358_at
4.425
NM_030889
Sorcs2

1418110_a_at
4.533
U39203
Inpp5d
1451746_a_at
4.424
AK016474
Atg12

1460223_a_at
4.531
NM_013514
Epb4.9
1454741_s_at
4.421
BG064061
AW547186

1418168_at
4.53
BB223737
Zcchc14
1455247_at
4.419
BB767281
Amotl1

1437366_at
4.525
BB332542
AI608492
1430614_at
4.412
BG962576
4632415K11Rik

1455074_at
4.521
AV307860

1435078_at
4.411
BQ173895
3526402J09Rik

1453077_a_at
4.521
AK016168
Snapc3
1452269_at
4.411
AF026489
Spnb3

1434032_at
4.519
BQ175381

1427930_at
4.405
BG063905
Pdxk

1417709_at
4.519
NM_010010
Cyp46a1
1420810_at
4.403
NM_019769
1500003O03Rik

1428924_at
4.514
AU016306
Mocs3
1417829_a_at
4.401
NM_134050
Rab15

1453428_at
4.514
AK012380
2700045P11Rik
1416287_at
4.401
NM_009062
Rgs4

1423195_at
4.512
BM208582
Hiat1
1422473_at
4.398
BM246564
Pde4b

1450918_s_at
4.503
BG868120
Src
1455961_at
4.397
AV174022
Mme

1457695_at
4.502
C76297
Ap1gbp1
1436513_at
4.396
BM208226
3526402J09Rik

1451028_at
4.501
AA796998
Baiap2
1431711_a_at
4.393
AK018497
9030409G11Rik

1442558_at
4.501
BB180191

1425452_s_at
4.388
BC002154
Ptprj; AW125753

1438067_at
4.497
BB526552
Nf1
1432184_a_at
4.387
AK011884
2610204M08Rik

1459107_at
4.496
BG069378
Kcnh3
1433699_at
4.387
BM241351
Tnfaip3

1438039_at
4.493
BE630599
Hectd1
1417225_at
4.386
NM_022992
Arl6ip5

1433526_at
4.493
BM942651
Klhl8
1436013_at
4.384
A1852434
C230098I05Rik

1460191_at
4.49
NM_019661
0610042I15Rik
1455156_at
4.382
BG519214
Strn

1447771_at
4.49
AW050081

1448378_at
4.382
NM_007984
Fscn1

1442883_s_at
4.488
BI413749
D10Bwg1364e
1437390_x_at
4.378
AV339210
Stx1a

1455023_at
4.487
BB171181
N28178
1455883_a_at
4.375
BB269910
Lrrtm1

1428550_at
4.486
AK007503
1810015A11Rik
1431875_a_at
4.374
AK017841
E2f1

1426643_at
4.485
AK012072
Elp3
1455888_at
4.369
BB125202
Lrrn6c

1453317_a_at
4.484
AK014353
Khdrbs3
1457046_s_at
4.369
BB545241
C77370

1460436_at
4.479
BI652065
Ndst1
1454977_at
4.365
AW551191
AU020772

1429124_s_at
4.477
BQ178367
Rexo1
1432271_a_at
4.364
AK012764
Dcun1d5

1418235_at
4.477
AV168389
Atg5
1423606_at
4.36
BI110565
Postn

1427058_at
4.475
AK010644
Eif4a1
1455548_at
4.357
BB314358
Dlgap4

1453412_a_at
4.475
BI652727
Sec14l1
1436081_a_at
4.354
BE632205
Zfp414

1425104_at
4.473
BC022615
Kctd1
1425710_a_at
4.352
AB019479
Homer1

1430612_at
4.471
BB533148
1810033B17Rik
1416528_at
4.349
NM_080559
Sh3bgrl3

1426972_at
4.471
AK009425
Sec24d
1450038_s_at
4.346
AW107303
Usp9x

1453021_at
4.345
BM899291
Stxbp5
1432091_a_at
4.307
AK007455
Rutbc3

1454675_at
4.344
BI076689
Thra
1448196_at
4.305
NM_134017
Mat2b

1440455_at
4.341
AI848599
AI848599
1431811_a_at
4.303
AK020018
Fbxo34

1433499_at
4.333
BF585125
2010005J08Rik
1458133_at
4.302
BB360008


1432478_a_at
4.333
AK015966
Ibrdc3
1457080_at
4.302
BB046159


1429678_at
4.325
AK017758
5730508B09Rik
1449932_at
4.3
NM_027874
Csnk1d

1460563_at
4.317
BE949414

1433937_at
4.298
BB814564
Trp53bp2

1424418_at
4.316
BC010801
BC010801
1455772_at
4.296
BB428874
Pgr

1452925_a_at
4.314
AK009364
5-Mar
1453069_at
4.292
BF018155
Pik3cb

1428354_at
4.312
BM206907
Foxk2
1416983_s_at
4.289
AI462296
Foxo1

1417481_at
4.311
NM_016894
Ramp1
1432813_at
4.289
AK013749
2900064F13Rik

1422677_at
4.311
AK002443
Dgat2
1436786_at
4.289
AV024133
1110069O07Rik

1415975_at
4.31
AU080787
Carhsp1
1458967_at
4.289
BM939341


1456601_x_at
4.309
AV002675
Fxyd2
1425327_at
4.288
AU067643
BC008163

1441917_s_at
4.307
BB468188
Tmem40



ProbeSymbol = Affymetrix probe identification corresponding to gene symbol;

FEN = fold enrichment when compared to reference sample;

Genbank = Genbank identification;

GeneSymbol = official gene symbol.




















































































































































































































































































































































































































































































































































TABLE 18



Top 1,000 striatopallidal (D2) cell translated mRNAs enriched when compared to a reference sample










ProbeSymbol
FEN
Genbank
GeneSymbol
ProbeSymbol
FEN
Genbank
GeneSymbol












1435211_at
183.2
BB554351
Ttc12
1449425_at
16.64
BC026373
Wnt2

1426814_at
104.8
BM248309
AU024582
1422079_at
16.52
NM_008856
Prkch

1452217_at
88.94
BE570050
Ahnak
1437698_at
15.91
AV370579
Myo5b

1440148_at
80.24
AI852874
Gpr6
1424507_at
15.72
BC011277
Rin1

1418950_at
72.42
NM_010077
Drd2
1454581_at
15.58
AK019908
5330425B07Rik

1419231_s_at
65.33
NM_010661
Krt1-12
1433566_at
15.41
BB381618
Rasl10b

1418357_at
52.9
NM_008241
Foxg1
1441368_at
15.29
BB102769


1426815_s_at
44.02
BM248309
AU024582
1452966_at
15.02
AK020296
9130430L19Rik

1457132_at
43.55
BF456117

1450072_at
14.94
BG694892
Ash1l

1443287_at
42.58
BB555669
Gm1337
1455190_at
14.83
BM114283
Gng7

1438022_at
40.99
BQ266518
Rab11fip3
1419230_at
14.66
NM_010661
Krt1-12

1435564_at
40.44
BB547893
C230078M08Rik
1449420_at
14.07
NM_008800
Pde1b

1436268_at
37.26
AI841578
Ddn
1427807_at
14.06
BC017159
4930448N21Rik

1458499_at
36.61
AW123977
Pde10a
1452879_at
13.95
AI848603
Synpo2

1421365_at
32.61
NM_008046
Fst
1452176_at
13.95
BB292874
Nup153

1454867_at
32.18
BB234631
Mn1
1425870_a_at
13.88
AF439339
Kcnip2

1420437_at
31.55
NM_008324
Indo
1434653_at
13.48
AV026976
Ptk2b

1460710_at
28.67
BB748999

1418619_at
13.42
NM_008319
Icam5

1423171_at
27.79
BE947345
Gpr88
1458342_at
13.38
BB313069


1423117_at
24.88
BB837171
Pum1
1436566_at
13.32
AV364488
Rab40b

1434458_at
23.89
BB444134
Fst
1435227_at
12.96
BM117007


1450142_a_at
21.46
NM_130886
Card14
1451280_at
12.87
BB159263
MGI: 107562

1434917_at
20.15
BQ173923
Cobl
1425503_at
12.63
AB037596
Gcnt2

1450339_a_at
19.88
NM_021399
Bcl11b
1454043_a_at
12.44
AK015412
Kcnab1

1435366_at
19.68
BB486367
D430042O09Rik
1436650_at
12.37
AV241894
Filip1

1427017_at
19.47
BB104560
Satb2
1449241_at
12.35
NM_053105
Klhl1

1460325_at
19.42
BB837171
Pum1
1456054_a_at
12.32
BB314559
Pum1

1439854_at
18.58
BQ175572
AI838259
1423530_at
12.24
BB320288
Stk32c

1423802_at
18.24
BC017634
Camky
1452202_at
12.21
BG069616
Pde2a

1450071_at
17.88
BG694892
Ash1l
1444734_at
12.08
BB183877
A330001L22Rik

1418782_at
17.83
NM_009107
Rxrg
1417356_at
12.04
AB003040
Peg3

1436275_at
17.8
AW490636
Kcnip2
1433627_at
11.96
AW546839
Sec23ip

1457143_at
17.66
BB322292
Gli3
1418847_at
11.93
NM_009705
Arg2

1456283_at
11.85
AV346211
Neto1
1452298_a_at
8.862
AW546331
Myo5b

1450026_a_at
11.85
AV306734
B3gnt1
1451917_a_at
8.818
AF155820
Dcamkl1

1435615_at
11.83
BB277790
Zfp365
1424037_at
8.796
BC027291
Itpka

1455683_a_at
11.73
BB451404
Tbc1d8
1422621_at
8.772
BM507707
Ranbp2

1436329_at
11.4
AV346607
Egr3
1443334_at
8.709
BB487579
D430042O09Rik

1428338_at
11.3
AK019166
2610039E05Rik
1422153_a_at
8.653
NM_026853
Asb11

1424248_at
11.23
BB159263
MGI: 107562
1415904_at
8.636
BC003305
Lpl

1434521_at
11.2
BB148972
Rfxdc2
1439552_at
8.633
BE985618
Trio

1427358_a_at
11.19
BC026671
Dapk1
1437460_x_at
8.63
BB264363
Rin1

1435083_at
11.09
BI155559
Ctxn1
1427683_at
8.629
X06746
Egr2

1451331_at
11.09
BC026568
Ppp1r1b
1460136_at
8.61
AI462839


1417090_at
10.95
NM_009037
Rcn1
1448990_a_at
8.524
AI255256
Myo1b

1457311_at
10.92
AW490258
Camk2a
1427931_s_at
8.518
BG063905
Pdxk

1460038_at
10.84
BG065255
Pou3f1
1429089_s_at
8.504
BG063749
2900026A02Rik

1451236_at
10.81
BC026463
Rerg
1427673_a_at
8.502
Z93948
Sema3e

1448807_at
10.78
NM_133849
Hrh3
1419224_at
8.47
NM_033567
Cecr6

1417804_at
10.75
NM_011242
LOC381240
1422068_at
8.454
NM_011141
Pou3f1

1452476_at
10.64
W41214
Cacnb2
1429285_at
8.419
AK009343
Serpina9

1438355_at
10.63
AI414870

1460244_at
8.419
NM_133995
Upb1

1418015_at
10.61
BI689507
Pum2
1434295_at
8.405
BE691356
Rasgrp1

1458406_at
10.6
BG144063

1438985_x_at
8.393
BB158937
Otud5

1427005_at
10.53
BM234765
Plk2
1438889_at
8.391
BB183456


1426980_s_at
10.29
BC006054
E130012A19Rik
1434248_at
8.328
BM243756
Prkch

1450213_at
10.17
NM_013875
Pde7b
1456401_at
8.318
BB078175
Cacnb2

1442166_at
10.03
BB273427
Cpne5
1419696_at
8.287
NM_013488
Cd4

1435749_at
9.907
AW911807
Gda
1441733_s_at
8.245
C88147
Nup153

1423561_at
9.752
AI838010
Nell2
1421141_a_at
8.234
BG962849
Foxp1

1456640_at
9.723
AW910872
Sh3rf2
1430127_a_at
8.229
AK007904
Ccnd2

1455754_at
9.708
BQ043839
Lmo3
1454721_at
8.185
AV257687
1110018G07Rik

1428150_at
9.671
BB203098
Coro7
1454768_at
8.147
AV337635
Kcnf1

1423186_at
9.628
BM228957
Tiam2
1421140_a_at
8.143
BG962849
Foxp1

1444681_at
9.613
BB749686
D14Ertd171e
1437586_at
8.123
BB756908
Cnot4

1454906_at
9.596
BB266455
Rarb
1455629_at
8.11
BE957273
Drd1a

1425833_a_at
9.537
AF326551
Hpca
1460343_at
8.092
AF401228
Neurl

1418169_at
9.532
BB223737
Zcchc14
1418881_at
8.09
NM_054095
Efcbp2

1418714_at
9.511
NM_008748
Dusp8
1435741_at
8.074
BB312125
Pde8b

1425756_at
9.479
AF425643
Rab40b
1455701_at
8.033
B1965517
Snx26

1433701_at
9.466
BB371430
Mpped1
1434008_at
8.029
BE993937
Scn4b

1448327_at
9.366
NM_033268
Actn2
1435748_at
8.028
AW911807
Gda

1419517_at
9.345
NM_028408
Cnih3
1460555_at
8.026
BM242294
6330500D04Rik

1441000_at
9.343
BB076832

1435221_at
7.968
BM220880
Foxp1

1451604_a_at
9.334
BC014291
Acyrl1
1436532_at
7.932
BB326709
Dcamkl3

1422710_a_at
9.333
NM_021415
Cacna1h
1450061_at
7.921
BM120053
Enc1

1440204_at
9.247
AW494150
3110039M20Rik
1454845_x_at
7.898
AW049955
Gpr24

1417680_at
9.208
NM_008419
Kcna5
1423544_at
7.878
BB188812
Ptpn5

1438799_at
9.154
AV338180
Dlx6os1
1457341_at
7.864
BE991676


1433815_at
9.13
AV290082
MGI: 1923321
1427343_at
7.862
BC026377
Rasd2

1418373_at
9.111
NM_018870
Pgam2
1416996_at
7.811
BC005421
Tbc1d8

1458407_s_at
9.046
BG144063

1417355_at
7.81
AB003040
Peg3

1434115_at
9.043
BQ176681

1452135_at
7.783
AV001252
Gpx6

1449158_at
8.965
NM_010607
Kcnk2
1451517_at
7.723
AF420001
Rhobtb2

1435334_at
8.945
BG866904
Ttc7
1428547_at
7.718
AV273591
Nt5e

1438665_at
8.935
BF456582
Smpd3
1436738_at
7.707
AV094878
AI449441

1437125_at
8.93
BB476448
Camk2a
1418691_at
7.696
NM_011268
Rgs9

1460567_at
8.928
BB148972
Rfxdc2
1455361_at
7.674
AW493391
Dgkb

1435780_at
8.914
BG966595
Psd
1452507_at
7.668
AF022078
Dlx6

1422706_at
7.638
AV370981
Tmepai
1452452_at
6.863
M73818


1456858_at
7.624
BB075339
Gpr149
1420580_at
6.861
NM_026249
4930429B21Rik

1427519_at
7.603
BG311385
Adora2a
1416350_at
6.855
NM_078477
Klf16

1450748_at
7.6
NM_021491
Smpd3
1424409_at
6.851
BC019534
Cldn23

1420146_at
7.544
AI429207
Tiam2
1455272_at
6.83
BB429139
Grm5

1421739_a_at
7.531
NM_010768
Matk
1416456_a_at
6.824
BC011134
Chia

1438841_s_at
7.492
AV002218
Arg2
1457052_at
6.783
BG064867
AW536275

1427015_at
7.466
BI732921
LOC380969
1428951_at
6.765
AK017551
Nol8

1418908_at
7.398
NM_013626
Pam
1431936_a_at
6.749
AK009828
Neu2

1419221_a_at
7.395
NM_016758
Rgs14
1439767_at
6.715
BB130159
Dlgap2

1459299_at
7.39
AV381193
A430065P19Rik
1419216_at
6.709
NM_009734
Azi1

1425479_at
7.377
BF160651
Smyd5
1455564_at
6.703
BQ176236
Bcr

1422165_at
7.374
X66603
Pou3f4
1429617_at
6.702
BM119209
Cyld

1416804_at
7.373
NM_053252
LOC114601
1447178_at
6.679
BB173057


1454762_at
7.368
BM932447
Xkrx
1444441_at
6.676
AW490909


1427344_s_at
7.364
BC026377
Rasd2
1421101_a_at
6.671
NM_010698
Ldb2

1420852_a_at
7.355
AV306734
B3gnt1
1427975_at
6.648
AK008807
2210403B10Rik

1433767_at
7.344
AV257687
1110018G07Rik
1421000_at
6.647
AI448404
Cnot4

1452368_at
7.313
AI853148
Bcr
1420545_a_at
6.634
NM_029716
Chn1

1416691_at
7.294
NM_019581
Gtpbp2
1445539_at
6.63
BE687857
Pde7b

1426915_at
7.292
BC021490
Dapk1
1419542_at
6.615
NM_010021
Dazl

1454752_at
7.276
AV307961
AI606861
1439557_s_at
6.585
BB097063
Ldb2

1417279_at
7.259
NM_010585
Itpr1
1436998_at
6.578
BB428991
Ankrd43

1456336_at
7.245
BB182912
A330102K23Rik
1424796_at
6.575
BC024705
1700054N08Rik

1419389_at
7.231
BQ180352
Pde10a
1456468_x_at
6.555
BB189996
1110012N22Rik

1455447_at
7.218
BM116882
D430019H16Rik
1455301_at
6.535
BG064092
BQ952480

1450690_at
7.212
BM507707
Ranbp2
1422718_at
6.531
NM_009682
Ap3s2

1422705_at
7.205
AV370981
Tmepai
1456051_at
6.527
BB282271
Drd1a

1455200_at
7.202
BB818370
Pak6
1456818_at
6.464
BB279083
Stk32a

1422678_at
7.198
AK002443
Dgat2
1427523_at
6.461
AI509029
Six3

1436066_at
7.162
BI965477
Kalrn
1423240_at
6.423
BG868120
Src

1447359_at
7.13
AI326876
LOC381955
1449634_a_at
6.416
AW046296
C030032C09Rik

1423231_at
7.123
AK002933
Nrgn
1426901_s_at
6.376
BB657856
Camta2

1436408_at
7.113
BE946298

1440534_at
6.373
BB177862


1420978_at
7.112
NM_010938
Nrf1
1438417_at
6.362
BB809239
Pwwp2

1436216_s_at
7.097
BM234799
2610204M08Rik
1426572_at
6.359
BM235734
Me2

1451726_at
7.091
BC020019
Mtmr6
1452380_at
6.348
BB075797
Epha7

1442434_at
7.087
BM195829
D8Ertd82e
1422208_a_at
6.347
BC016135
Gnb5

1453782_at
7.086
BI737125
3021401C12Rik
1435435_at
6.333
BB357580
Cttnbp2

1448364_at
7.072
U95826
Ccng2
1454877_at
6.319
BQ174721
Sertad4

1435296_at
7.051
AV349563
Adra2c
1419754_at
6.298
NM_010864
Myo5a

1438210_at
7.049
BB126999
Gpr149
1420666_at
6.296
BM117900
Doc2b

1416776_at
7.04
NM_016669
Crym
1424028_at
6.292
BCO23107
5830457O10Rik

1432073_at
7.007
AK007198
1700113I22Rik
1417440_at
6.266
NM_033566
Arid1a

1432972_at
7.004
AK014916
4921518B13Rik
1453886_a_at
6.265
AK017037
Slc25a26

1426518_at
6.977
AV333951
Tubgcp5
1456946_at
6.262
BE949337
4831416G18Rik

1427045_at
6.977
AI849322
Synpo
1420377_at
6.23
BG071333
St8sia2

1429156_at
6.97
BF453953
2610036L11Rik
1443365_at
6.216
BB431008
Htr4

1417129_a_at
6.954
U68384
Mrg1
1416437_a_at
6.213
AF262046
Mapk8ip3

1429422_at
6.945
AK016788
4933412E12Rik
1427682_a_at
6.21
X06746
Egr2

1425130_a_at
6.941
U28216
Ptpn5
1448978_at
6.205
NM_019867
Ngef

1421353_at
6.94
NM_013875
Pde7b
1454656_at
6.19
AV271736
Spata13

1439569_at
6.925
AV291031
Gpr83
1428535_at
6.16
AK004276
9430020K01Rik

1430675_at
6.915
BM932606
2900055J20Rik
1455178_at
6.142
BB496468
Rutbc1

1452618_at
6.887
BM230774
Cdk5rap2
1416562_at
6.137
AF326547
Gad1

1439566_at
6.882
BB245373
C030038J10Rik
1448709_at
6.131
NM_033566
Arid1a

1457979_at
6.128
BM938335

1453836_a_at
5.523
AK006949
Mgll

1435666_at
6.098
AW553439

1416050_a_at
5.505
NM_016741
Scarb1

1433583_at
6.093
AV327248
Zfp365
1420985_at
5.46
BG694892
Ash1l

1422018_at
6.075
NM_010437
Hivep2
1450655_at
5.455
AA214868
Herc1

1455056_at
6.068
BM231903
Lmo7
1450042_at
5.44
BB322201
Arx

1436092_at
6.045
BB336256

1417626_at
5.435
NM_031401
Usmg4

1455296_at
6.031
BE952286
Adcy5
1421116_a_at
5.427
NM_024226
Rtn4

1420534_at
6.016
AK004815
Gucy1a3
1438784_at
5.42
BB329234


1421142_s_at
6.012
BG962849
Foxp1
1434775_at
5.419
AW543460
Pard3

1427450_x_at
6.007
BI080370
Myo1b
1419390_at
5.411
BQ180352
Pde10a

1434429_at
6.002
AV348245
Syt16
1418017_at
5.398
BI689507
Pum2

1423420_at
5.998
AK018378
Adrb1
1418535_at
5.387
NM_016846
Rgl1

1416627_at
5.974
NM_016907
Spint1
1438068_at
5.378
BB251859


1435808_at
5.95
BB174377
A230051G13Rik
1435825_at
5.364
BG969012
Acvrl1

1440711_at
5.93
BB426320
C630001G18Rik
1431056_a_at
5.359
AK017272
Lpl

1435763_at
5.899
BI218684
Tbc1d16
1417612_at
5.347
BF147705
Ier5

1417114_at
5.894
AF163665
Gdl
1420871_at
5.339
BF472806
Gucy1b3

1453129_a_at
5.892
AK004813
Rgs12
1423532_at
5.337
AI850285
Rnf44

1417709_at
5.881
NM_010010
Cyp46a1
1438664_at
5.337
BB216074
Prkar2b

1453264_at
5.87
AK007346
Marveld3
1417812_a_at
5.325
NM_008484
Lamb3

1455703_at
5.867
BG074736
Akt2
1447861_x_at
5.322
AV329643
Mrg1

1427912_at
5.858
AK003232
Cbr3
1419033_at
5.32
AW556821
2610018G03Rik

1419137_at
5.825
NM_021423
Shank3
1455220_at
5.317
BB353860
Frat2

1455620_at
5.814
BQ177219

1435767_at
5.314
BE951842
Scn3b

1429111_at
5.808
AV270892
Tln2
1435285_at
5.312
BB731805
Mpped2

1433988_s_at
5.808
BG075755
C230098O21Rik
1439611_at
5.305
BE947522
Chrm1

1435210_s_at
5.785
BQ174936
Snph
1448063_at
5.302
AW226526
Iqsec2

1434090_at
5.771
BB037094
4930429A22Rik
1427426_at
5.295
AV362204
Kcnq5

1450143_at
5.766
BB354696
Rasgrp1
1421028_a_at
5.291
AI595932
Mef2c

1435265_at
5.763
BF466929

1421346_a_at
5.268
NM_009320
Slc6a6

1441963_at
5.744
BB427703
MGI: 2656976
1430013_at
5.263
BE993118
2210008F06Rik

1448023_at
5.741
BB662566
Kalrn
1452964_at
5.26
AK016577
4932702F08Rik

1447771_at
5.706
AW050081

1422787_at
5.259
NM_019873
Fkbpl

1416286_at
5.696
NM_009062
Rgs4
1450227_at
5.254
BM199504
Ankrd6

1460206_at
5.688
NM_019518
Grasp
1426450_at
5.25
BM207017
Plcl2

1423376_a_at
5.685
AV341904
Dok4
1441468_at
5.242
BB326028


1435558_at
5.683
BB351248
Bai2
1436733_at
5.239
BB523550
E130309F12Rik

1455961_at
5.675
AV174022
Mme
1429316_at
5.234
AK018120
Rasgef1a

1447017_at
5.661
BE956696

1440166_x_at
5.232
BB378627
Htr1d

1460285_at
5.647
NM_133721
Itga9
1455225_at
5.217
AV237615
Syne1

1432184_a_at
5.611
AK011884
2610204M08Rik
1435043_at
5.214
BB794831
Plcb1

1431751_a_at
5.605
AK012553
Mpped2
1417522_at
5.211
AF441120
Fbxo32

1422902_s_at
5.594
NM_023799
Mgea5
1448468_a_at
5.207
AF033003
Kcnab1

1431394_a_at
5.59
AK014938
Lrrk2
1452295_at
5.207
AV291712
Tmepai

1434359_at
5.589
AV329070
6330500D04Rik
1426037_a_at
5.192
U94828
Rgs16

1445691_at
5.588
AW121426
Chn1
1434745_at
5.186
BQ175880
Ccnd2

1435071_at
5.583
AV327165
Zfyve1
1447728_x_at
5.183
BB718260
Hspa9a

1425510_at
5.578
BM213279
Mark1
1426024_a_at
5.177
AF187147
Dbn1

1427038_at
5.577
M13227
Penk1
1448989_a_at
5.176
AI255256
Myo1b

1417462_at
5.576
NM_007598
Cap1
1416398_at
5.172
BB474887
Mesdc1

1433937_at
5.559
BB814564
Trp53bp2
1429637_at
5.163
AK008987
2210419I08Rik

1415743_at
5.537
NM_010412
Hdac5
1450930_at
5.161
AK002992
Hpca

1452861_at
5.534
AK008485
2010300C02Rik
1442558_at
5.16
BB180191


1453273_at
5.533
AV142265
Kcnv1
1433962_at
5.153
BB131965
6720458F09Rik

1419358_at
5.53
NM_030889
Sorcs2
1441894_s_at
5.148
BB071890
Grasp

1460352_s_at
5.526
BC017537
Pik3r4
1447707_s_at
5.127
BB367174
Pde2a

1433451_at
5.109
BB177836
Cdk5r1
1438224_at
4.663
BE572265
Zswim5

1422609_at
5.084
BE648432
Arpp19
1448598_at
4.662
NM_011846
Mmp17

1420530_at
5.083
AW553317
Neud4
1415834_at
4.658
NM_026268
Dusp6

1432646_a_at
5.076
BE859789
2900097C17Rik
1435218_at
4.656
BI134758
Rasgef1a

1423285_at
5.065
BB731671
Coch
1416683_at
4.647
NM_138749
Plxnb2

1435605_at
5.039
BB125424
Actr3b
1416983_s_at
4.643
AI462296
Foxo1

1451483_s_at
5.039
BC024705
1700054N08Rik
1437929_at
4.642
AV306847
Dact2

1428321_at
5.027
AK003593
Emil1
1452092_at
4.641
AK019474
4631426J05Rik

1448842_at
5.02
NM_033037
Cdo1
1433450_at
4.634
BB177836
Cdk5r1

1420496_at
4.998
NM_021489
F12
1435392_at
4.631
BB277182
Wdr17

1442113_at
4.991
BB183350
5330417C22Rik
1451475_at
4.628
BC019530
Plxnd1

1451078_at
4.988
BC012878
2510039O18Rik
1420981_a_at
4.628
NM_010723
Lmo4

1459679_s_at
4.975
AA406997
Myo1b
1418144_a_at
4.62
NM_008847
Pip5k1b

1425363_at
4.931
BC022180
Galgt1
1452917_at
4.614
AK011489
Rfc5

1434141_at
4.928
BG072799
Gucy1a3
1425466_at
4.611
AV317107
Senp2

1454832_at
4.917
BG228702
Phactr1
1421622_a_at
4.611
NM_019688
Rapgef4

1449863_a_at
4.911
NM_010056
Dlx5
1434153_at
4.603
BI408715
Shb

1434077_at
4.896
AV222037
Wdr37
1422977_at
4.594
NM_010327
Gp1bb

1423358_at
4.89
AK007407
1810009K13Rik
1455463_at
4.594
BB427286
Phyhip

1424379_at
4.877
BC019393
Car11
1422473_at
4.59
BM246564
Pde4b

1431320_a_at
4.872
AK002362
Myo5a
1426553_at
4.579
AK017830
Dnajc14

1435222_at
4.872
BM220880
Foxp1
1436002_at
4.579
BB484128
C230013L11Rik

1456786_at
4.87
BB097063
Ldb2
1435482_at
4.575
BB318003
Fibcd1

1454742_at
4.87
BB003229
Rasgef1b
1452851_at
4.573
AK019578
Tnrc4

1424201_a_at
4.864
AW537349
Seh1l
1426354_at
4.57
AK009033
Bap1

1423658_at
4.86
BC023131
Sppl3
1441764_at
4.562
BF730739
Prdm10

1441884_x_at
4.854
BB278992
Cx3cl1
1454759_at
4.56
AV230461
Git1

1422901_at
4.85
NM_023799
Mgea5
1434825_at
4.556
BQ175287
LOC381742

1440940_at
4.845
BE949510
Cacnb1
1437017_at
4.556
BB046727
AI480653

1448724_at
4.841
NM_009895
Cish
1435128_at
4.546
BB429313
Baiap2

1436645_a_at
4.84
BB066603
Cnot4
1419066_at
4.546
NM_030244
Ier5l

1429438_at
4.838
AV318805
Bcor
1448805_at
4.545
NM_009480
Usf1

1420872_at
4.79
BF472806
Gucy1b3
1437018_at
4.536
BG072348
Pnma2

1458133_at
4.784
BB360008

1439618_at
4.531
AI448308
Pde10a

1418172_at
4.778
AF117613
Hebp1
1437001_at
4.527
BQ173949
Gsk3b

1415803_at
4.775
AF010586
Cx3cl1
1421048_a_at
4.521
NM_023249
Ypel1

1423551_at
4.771
BB776961
Cdh13
1455881_at
4.52
BB078200
Ier5l

1419225_at
4.768
NM_009785
Cacna2d3
1449188_at
4.52
NM_021565
Midn

1423489_at
4.764
BC021914
Mmd
1428540_at
4.509
BE944524
3321401G04Rik

1435500_at
4.756
BB270778
Rab26
1426454_at
4.507
AK002516
Arhgdib

1449472_at
4.747
NM_008151
Gpr12
1429124_s_at
4.502
BQ178367
Rexo1

1457651_x_at
4.737
BB270375
Rem2
1425617_at
4.501
U91922
Dhx9

1424899_at
4.734
BC005737
Nmnat3
1417207_at
4.497
BF466091
Dvl2

1416982_at
4.725
AI462296
Foxo1
1422762_at
4.489
AB008867
Kif17

1423640_at
4.718
BC026512
Synpr
1451630_at
4.486
BC018513
Ttl

1441065_at
4.717
BB305753

1457632_s_at
4.485
BB207647
Mrg1

1427006_at
4.717
BB339051
Rapgef1
1443036_at
4.477
BG073535
C630007C17Rik

1455313_at
4.716
BB336053
Ablim2
1446840_at
4.475
BQ032894


1450448_at
4.714
BQ032752
Stc1
1449468_at
4.468
NM_012028
St6galnac5

1452974_at
4.706
AK017551
Nol8
1423415_at
4.463
BB110067
Gpr83

1452411_at
4.692
BG966295
Lrrc1
1441928_x_at
4.461
BB139475
Ell

1425680_a_at
4.692
AF110396
Btrc
1428950_s_at
4.461
AK017551
Nol8

1417341_a_at
4.692
NM_025800
Ppp1r2
1431230_a_at
4.461
Z74630
Btbd9

1430617_at
4.68
BB238604
Oip5
1424475_at
4.459
BI157430
Camkk2

1434594_at
4.679
BB497449
Dnahc1
1448687_at
4.457
NM_026125
C1qdc2

1428095_a_at
4.663
AK004920
Tmem24
1428864_at
4.456
AK017419
5530400B01Rik

1437378_x_at
4.45
BB224405
Scarb1
1460717_at
4.252
AF042180
Tspyl1

1423785_at
4.448
BE995700
Egln1
1437579_at
4.246
C77054
Nek2

1442370_at
4.436
BB267006

1431920_a_at
4.234
AK008082
2010004A03Rik

1424175_at
4.433
BC017689
Tef
1442708_at
4.225
BB504418


1460718_s_at
4.433
AF192558
Mtch1
1424759_at
4.224
BC017528
Arrdc4

1436100_at
4.431
AV347995
Sh2d5
1441786_at
4.217
AW048005


1440479_at
4.431
BB821623
Cbx4
1430037_at
4.216
AK017836
Snx27

1451696_at
4.43
BC004695
Zfp64
1426978_at
4.213
AW682368
Klhl2

1426585_s_at
4.429
BM209765
Mapk1
1455555_at
4.212
BF224468
AV071699

1439548_at
4.428
BB390705
Rap2b
1443888_at
4.206
BB426608


1436754_at
4.42
BB360523
AI839735
1451507_at
4.205
BB280300
Mef2c

1433707_at
4.419
BB430205
Gabra4
1435441_at
4.204
BB336053
Ablim2

1451285_at
4.416
AF224264
Fus
1435845_at
4.2
BB764479
2900006N09Rik

1439422_a_at
4.414
AV048291
C1qdc2
1415985_at
4.199
NM_133953
Sf3b3

1456755_at
4.388
AI467545
2310001H13Rik
1448747_at
4.194
AF441120
Fbxo32

1416537_at
4.386
NM_133930
Creld1
1421897_at
4.194
AI385733
Elk1

1423478_at
4.377
BF660388
Prkcb1
1416402_at
4.184
AV382118
Abcb10

1424480_s_at
4.374
BC026151
Akt2
1426465_at
4.175
BG066219
Dlgap4

1422751_at
4.374
NM_011599
Tle1
1455771_at
4.174
BB467799
Bzrap1

1417456_at
4.372
NM_010322
Gnpat
1430128_a_at
4.169
AK002562
Dp1l1

1418168_at
4.371
BB223737
Zcchc14
1437865_at
4.168
AW546433
Spata13

1448656_at
4.365
NM_007581
Cacnb3
1427300_at
4.167
D49658
Lhx8

1426241_a_at
4.358
AB030906
Scmh1
1440258_at
4.165
BE951512
Kcnq2

1423049_a_at
4.352
AK002271
Tpm1
1426012_a_at
4.147
BC010499
2610301G19Rik

1426900_at
4.35
AV293164
Jmjd1c
1434805_at
4.147
BG063049
Mllt1

1458443_at
4.345
BE986758
Mect1
1417524_at
4.144
NM_009920
Cnih2

1426822_at
4.344
BM201133
Rhot2
1455772_at
4.141
BB428874
Pgr

1417461_at
4.335
NM_007598
Cap1
1424384_a_at
4.14
BC006765
Znrf1

1427674_a_at
4.33
D29763
Sez6
1416448_at
4.137
NM_025922
Itpa

1450835_a_at
4.329
NM_020014
Gfra4
1435204_at
4.129
BG065273
Hrmt1l4

1454844_at
4.325
AW049955
Gpr24
1422527_at
4.123
NM_010386
H2-DMa

1421962_at
4.324
A1664344
Dnajb5
1455548_at
4.122
BB314358
Dlgap4

1424645_at
4.32
BB795102
Tnrc6c
1423694_at
4.121
BC006935
Kctd10

1455080_at
4.317
BB375209
Ppp1r16b
1428872_at
4.117
AW495537
4121402D02Rik

1423228_at
4.317
BG066773
B4galt6
1421175_at
4.116
NM_008666
Myt1l

1423200_at
4.317
U22016
Ncor1
1426897_at
4.115
AV122997
Rcc2

1417393_a_at
4.314
NM_026125
C1qdc2
1434762_at
4.114
BF457736
A730041015Rik

1418467_at
4.305
NM_025891
Smarcd3
1436005_at
4.111
BQ174136
Sfrs14

1420427_a_at
4.296
NM_133941
Dhx32
1420738_at
4.109
NM_016793
Zfp98

1422407_s_at
4.294
NM_008284
Hras1
1423699_at
4.107
BC003900
D15Ertd785e

1425929_a_at
4.289
AF249668
Rnf14
1438663_at
4.106
BF730196
Bat2d

1451908_a_at
4.287
BC005766
Sec14l1
1435674_at
4.101
BB351953
Rhobtb2

1415998_at
4.284
NM_011694
Vdac1
1420718_at
4.093
NM_011856
0dz2

1428937_at
4.281
BI080417
Atp2b1
1454960_at
4.09
BI646741
Smad3

1417062_at
4.279
NM_026034
2810037C14Rik
1447947_at
4.089
BM220786
Zfyve16

1428148_s_at
4.271
BB203098
Coro7
1435598_at
4.089
BE996371
Shc2

1441369_at
4.27
BB329116

1416397_at
4.077
BB474887
Mesdc1

1431074_a_at
4.268
BI654600
Pitpnc1
1433526_at
4.076
BM942651
Klhl8

1441815_at
4.266
AI851453
AI851453
1460279_a_at
4.074
AF043219
Gtf2i

1442562_at
4.263
BB346556
D630040G17Rik
1453972_x_at
4.072
AK005207
Pparbp

1459920_at
4.262
AA163908
4933415E08Rik
1425679_a_at
4.066
AF109769
Mapk8ip1

1458399_at
4.261
AI413999
Lrrc3
1448553_at
4.063
NM_080728
Myh7

1448877_at
4.26
NM_010054
Dlx2
1429738_at
4.057
AK013847
Myt1l

1439866_at
4.255
BQ173950
Parc
1439497_at
4.056
BG065013
4933415E08Rik

1423522_at
4.254
BB811478
Npm3
1428443_a_at
4.048
AK005063
Rap1ga1

1449214_a_at
4.252
BC025160
Opa1
1443694_at
4.045
BB794177
Rgs20

1421340_at
4.043
NM_008580
Map3k5
1428259_at
3.91
AK010185
2310075M15Rik

1430640_a_at
4.039
BI695530
Prkar2b
1440207_at
3.907
BI155142
AI1505034

1416605_at
4.036
BC024944
Nola2
1450853_at
3.904
AU045006
Tle4

1435206_at
4.036
BB767169
Slc24a4
1427579_at
3.904
BE653576
Rhbdl4

1428147_at
4.035
BB203098
Coro7
1426973_at
3.903
AK004377
Gpr153

1446312_at
4.034
BB273243

1439888_at
3.902
BB271581


1429174_at
4.032
AK014304
Wdr34
1427618_at
3.901
BQ176417
Cdh9

1428508_at
4.029
AK004526
1810061M12Rik
1435844_at
3.896
BB164247
A330009N23Rik

1456280_at
4.022
BG067086
Clspn
1426644_at
3.886
BC002196
Tbc1d20

1434563_at
4.021
BM207149
Rps6kc1
1436563_at
3.882
AV277283
4932441J04Rik

1440870_at
4.018
BB356786
Prdm16
1436381_at
3.88
BQ175774
Dlgap3

1454835_at
4.014
AV340515
Epm2aip1
1460552_at
3.878
AI327392
Ascc3l1

1422293_a_at
4.008
NM_134112
Kctd1
1454609_x_at
3.878
BB770958
6430527G18Rik

1452063_at
4.007
AK010736
2410081M15Rik
1429715_at
3.878
AK010380
Ppp2r2a

1425600_a_at
4.007
AW049748
Plcb1
1452911_at
3.872
AK017680
Spred1

1455242_at
3.999
BM220880
Foxp1
1455956_x_at
3.864
AV310588
Ccnd2

1456930_at
3.999
BE989461
Camsap1
1416981_at
3.864
AI462296
Foxo1

1433499_at
3.998
BF585125
2010005J08Rik
1456838_at
3.863
BE995608
LOC237403

1417216_at
3.997
NM_138606
Pim2;
1417749_a_at
3.862
NM_009386
Tjp1




LOC229005
1434969_at
3.859
BB381558


1440487_at
3.995
BE824778
Dcc
1421107_at
3.859
NM_021420
Stk4

1437400_at
3.992
BB309512
Nedd4l
1454875_a_at
3.857
BF011461
Rbbp4

1434904_at
3.991
AW321867
Hivep2
1422807_at
3.856
NM_007480
Arf5

1439867_at
3.987
BB079377
Ubox5
1460436_at
3.856
BI652065
Ndst1

1420961_a_at
3.983
NM_028582
Ivns1abp
1434082_at
3.851
BM243464
Pctk2

1421498_a_at
3.982
NM_023450
2010204K13Rik
1436513_at
3.85
BM208226
3526402J09Rik

1451008_at
3.982
BB360510
St8sia3
1452844_at
3.844
AK009674
Pou6f1

1429718_at
3.981
BB782729
Slitrk5
1422590_at
3.844
NM_007668
Cdk5

1451098_at
3.98
BC010524
Pcoln3
1453915_a_at
3.841
AK012071
Slc37a3

1433642_at
3.974
BB384173
Arfrp2
1443612_at
3.84
AW123199


1456785_at
3.973
BQ175889
Crsp2
1419080_at
3.831
NM_010275
Gdnf

1422255_at
3.97
NM_021275
Kcna4
1460568_at
3.819
BI156583
Trim46

1429104_at
3.964
AK012581
0610025L06Rik
1426643_at
3.815
AK012072
Elp3

1447642_x_at
3.96
AV354897
Dmwd
1435618_at
3.814
BB473446
Pnma2

1420999_at
3.958
AI448404
Cnot4
1422485_at
3.814
AK004804
Smad4

1459994_x_at
3.955
AV027486
Trfr2
1452057_at
3.807
BG801851
Actr1b

1455820_x_at
3.955
BB138434
Scarb1
1435125_at
3.804
BB303627
BB120497

1456606_a_at
3.949
AV259240
Chst11; Phactr1
1416122_at
3.801
NM_009829
Ccnd2

1455365_at
3.948
BB426483
Cdh8
1428839_at
3.8
AK005105
Wdr53

1455191_x_at
3.948
BB822856
Pip5k1b
1426770_at
3.799
AJ416473
Pex5

1440177_at
3.948
BM899529
9.63E+17
1452940_x_at
3.798
AI595920
Pitpnc1

1443073_at
3.945
BB355954
LOC545681
1441867_x_at
3.796
AI480494
4930534B04Rik

1422335_at
3.942
NM_007418
Adra2c
1423195_at
3.796
BM208582
Hiat1

1460223_a_at
3.942
NM_013514
Epb4.9
1456444_at
3.795
BB126849
Fbxo41

1433743_at
3.937
BG075820
Dach1
1433811_at
3.794
BG245272
Mllt6

1426785_s_at
3.931
BI411560
Mgll
1433747_at
3.793
BQ176475
Lnpep

1415976_a_at
3.93
AU080787
Carhsp1
1417305_at
3.793
NM_007463
Apeg1

1452938_at
3.924
BQ174247
C030032C09Rik
1457077_at
3.791
BB306048


1418323_at
3.921
BM232562
Fem1b
1428853_at
3.79
BG071079
Ptch1

1424132_at
3.92
BC011083
Hras1
1428852_at
3.788
AK018140
Dock3

1426487_a_at
3.918
BB092954
Rbbp6
1424150_at
3.788
BC024955
Gdpd5

1426108_s_at
3.917
AY094172
Cacnb1
1421191_s_at
3.786
NM_053187
Gopc

1426383_at
3.917
BF303057
Cry2
1427468_at
3.782
M81483
Ppp3cb

1428914_at
3.917
AK009333
2310014D11Rik
1418080_at
3.782
AW125856
B4galt2

1455279_at
3.911
BG070552
Gm1060
1453689_at
3.781
AK013325
Fance

1460610_at
3.91
BG143491
9430057O19Rik
1419234_at
3.781
BG070273
Helb

1460210_at
3.78
NM_013630
Pkd1
1428716_at
3.656
BG076082
Pex1

1428173_at
3.777
AK005562
Eml2
1451998_at
3.653
BC024597
4930485D02Rik

1435547_at
3.776
BQ175722
Mkl2
1431717_at
3.653
AK014386
3526401B18Rik

1428892_at
3.773
AK004331
Ppil1
1454685_at
3.649
BQ177047


1456766_at
3.773
BB036966
C330001K17Rik
1423269_a_at
3.649
BB663717
Nedd4l

1439662_at
3.771
BM124609
Homer1
1450910_at
3.647
AV261931
Cap2

1428786_at
3.77
BM238906
Nckap1l
1416535_at
3.644
BC003746
Mcrs1;

1445194_at
3.77
BB355006
Cnksr2



LOC433780

1438266_at
3.77
BB764453
Thsd6
1417032_at
3.644
AF296657
Ube2g2

1428926_at
3.769
AK003388
1110003O08Rik
1421312_a_at
3.643
NM_010630
Kifc2

1427071_at
3.767
BC003960
Fbxo42
1460009_at
3.639
BB667115
Ier5

1452149_at
3.766
AU067784
Ube3b
1416550_at
3.639
BB320416
Slc35b4

1422737_at
3.765
NM_008679
Ncoa3
1449168_a_at
3.638
BC003735
Akap2

1423842_a_at
3.764
AF305730
Rnf41
1418639_at
3.631
NM_011359
Sftpc

1426972_at
3.763
AK009425
Sec24d
1435504_at
3.63
BM217861
Rsnl2

1454659_at
3.762
BG069699
Dctd
1435019_at
3.629
BB532141
Atxn7l3

1423519_at
3.759
BE457744
2210412D01Rik
1423673_at
3.628
BC023053
Ldoc1l

1445359_at
3.756
BE993830
Adcy1
1437842_at
3.626
BB311508
Plcxd1

1447696_x_at
3.754
BB091427
Adcy5
1433787_at
3.623
AI841091
B230343H07Rik

1429228_at
3.754
BE980134
4930534B04Rik
1451568_at
3.62
BC025603
A630054L15Rik

1421817_at
3.754
AK019177
Gsr
1452624_at
3.618
BM900077
Lrrtm1

1440741_at
3.751
BB829587
Htr1d
1450520_at
3.617
NM_019430
Cacng3

1457919_at
3.739
BB446049
D030054H15Rik
1433699_at
3.617
BM241351
Tnfaip3

1456601_x_at
3.738
AV002675
Fxyd2
1433468_at
3.614
BB770958
6430527G18Rik

1452718_at
3.736
BG073486
Edd1
1418016_at
3.614
BI689507
Pum2

1442883_s_at
3.734
BI413749
D10Bwg1364e
1419248_at
3.612
AF215668
Rgs2

1417844_at
3.734
NM_026119
Med4
1429021_at
3.608
AK013481
Epha4

1420965_a_at
3.732
BM120053
Enc1
1427954_at
3.606
BC028785
BC048403

1455023_at
3.728
BB171181
N28178
1449932_at
3.605
NM_027874
Csnk1d

1452913_at
3.725
AV337888
Pcp4l1
1454985_at
3.604
BI872009
D030051N19Rik

1453428_at
3.72
AK012380
2700045P11Rik
1451722_s_at
3.602
BF160651
Smyd5

1432813_at
3.718
AK013749
2900064F13Rik
1429122_a_at
3.6
AK006658
1700040I03Rik

1433924_at
3.709
BM200248
Peg3
1424317_at
3.6
BC018167
Slc25a19

1438913_x_at
3.707
AW322533
Hdgfrp2
1447124_at
3.599
BE634581


1438213_at
3.705
BB128962
A830018L16Rik
1437989_at
3.598
BB357157
Pde8b

1429201_at
3.705
AK013508
Cyld
1448374_at
3.595
AK005130
Med28

1416125_at
3.699
U16959
Fkbp5
1424044_at
3.594
BC007145
Jmjd2b

1457213_a_at
3.698
AV276089
Dgkh
1434832_at
3.59
BB364488
Foxo3a

1416106_at
3.695
NM_029571
Kti12
1436483_at
3.589
BM116113
Myt1l

1442099_at
3.695
BM227490
Usp31
1453324_at
3.588
BF459399
6330509M23Rik

1421860_at
3.689
BG065300
Clstn1
1437750_at
3.586
BI133257
2310037P21Rik

1450366_at
3.689
NM_007545
Hrk
1443542_at
3.582
BB767151


1433777_at
3.688
BB152370
L3mbtl2
1429392_at
3.58
BI558553
Wdr40a

1416953_at
3.685
NM_010217
Ctgf
1456144_at
3.58
AW537064
Nav3

1426009_a_at
3.685
BC003763
Pip5k1b
1457456_at
3.577
AI481735
Map3k10

1422453_at
3.674
NM_138659
Prpf8
1419056_at
3.577
AF038538
Rtn2

1445854_at
3.674
BB380166
C230004F18Rik
1429832_at
3.575
AK014665
Ppih

1452438_s_at
3.672
BE632382
Taf4a
1458351_s_at
3.572
BB428573
Klhl2

1426069_s_at
3.671
BC016100
Slc7a4
1428936_at
3.572
BI080417
Atp2b1

1424047_at
3.67
BC016218
Dera
1418173_at
3.571
NM_133730
4631426H08Rik

1433987_at
3.666
AV153557
Hpcal4
1454728_s_at
3.569
AW610650
Atp8a1

1454700_at
3.665
BB093349
Lrfn4
1422474_at
3.568
BM246564
Pde4b

1448498_at
3.663
NM_019924
Rps6ka4
1451106_at
3.565
BC025499
Rbm21

1416863_at
3.663
NM_022419
Abhd8
1422677_at
3.564
AK002443
Dgat2

1428222_at
3.66
AK018179
Dcamkl2
1423973_a_at
3.562
BC024935
Arf3

1430413_at
3.66
AK018228
6330540D07Rik
1422799_at
3.562
AK019427
Bat2

1446681_at
3.562
AV327329
BB086117
1437580_s_at
3.453
C77054
Nek2

1451506_at
3.557
BB280300
Mef2c
1460262_a_at
3.451
NM_026091
1700037H04Rik

1429678_at
3.557
AK017758
5730508B09Rik
1434339_at
3.45
AW548221
Fnbp1l

1457361_at
3.555
BB628626
C630007C17Rik
1428777_at
3.447
AK017680
Spred1

1424117_at
3.552
BC024937
BC056474
1418743_a_at
3.447
NM_021344
MGI: 1930803

1436142_at
3.55
BM218877
3526401B18Rik
1450040_at
3.444
M93954
Timp2

1452467_at
3.549
BQ042988
Mmab
1455033_at
3.444
BB325849
B430201A12Rik

1434830_at
3.547
AV228517
Mxd1
1427930_at
3.444
BG063905
Pdxk

1428327_at
3.547
BB010301
2310001H13Rik
1457256_x_at
3.441
BB530125
Ptch2

1423442_a_at
3.545
BE854125
Fbxw2
1454092_a_at
3.441
AK017176
Gtf2h3

1423330_at
3.543
AK006149
Ensa
1427993_at
3.434
AI852705
Rufy2

1433621_at
3.536
BB334979
Wdr41
1448554_s_at
3.432
NM_080728
Myh6

1455395_at
3.536
BB446073
4933425A18Rik
1448445_at
3.431
AB030038
Acp6

1425180_at
3.535
AV344708
Sgip1
1426786_s_at
3.431
BM195397
Dhx38

1427091_at
3.532
BC025488
AI481105
1455401_at
3.428
AW061083
Camkk2

1423395_at
3.53
BM021706
Tsnax
1420694_a_at
3.425
BB522228
Dach1

1437921_x_at
3.53
AW744723
Zfp516
1416087_at
3.419
NM_007457
Ap1s1

1419577_at
3.524
NM_133999
A530089I17Rik
1449502_at
3.418
NM_010021
Dazl

1434154_at
3.523
BQ177107
Kctd13
1419583_at
3.416
NM_007625
Cbx4

1416292_at
3.521
NM_007452
Prdx3
1425415_a_at
3.414
U75214
Slc1a1

1438478_a_at
3.52
AI313926
Ppp3ca
1434902_at
3.413
BB163668
Rnf157

1434415_at
3.519
BB797871

1454827_at
3.41
BG070415
Pogz

1449494_at
3.514
AY026947
Rab3c
1435169_at
3.409
BB431047
A930001N09Rik

1424503_at
3.511
BC006596
Rab22a
1420533_at
3.409
AK004815
Gucy1a3

1455247_at
3.508
BB767281
Amotl1
1422431_at
3.408
NM_053201
Magee1

1417349_at
3.507
NM_019788
Pldn
1437214_at
3.408
BB667279
Lrrtm4

1452269_at
3.506
AF026489
Spnb3
1417829_a_at
3.406
NM_134050
Rab15

1460623_at
3.506
BB753881
Scap2
1455865_at
3.404
BB468410
Insm1

1429330_at
3.504
AK013727
Gabra4
1437980_at
3.404
BB814947
9130230N09Rik

1425580_a_at
3.504
BC024675
Pik3c3
1451273_x_at
3.404
BC025546
BC025546

1418895_at
3.502
NM_018773
Scap2
1434720_at
3.403
BF320240
9530033F24Rik

1428642_at
3.497
AK018094
Slc35d3
1419834_x_at
3.4
AW491150
Mark1

1428550_at
3.496
AK007503
1810015A11Rik
1434531_at
3.397
BB400317
GnT-IX

1452234_s_at
3.493
BF457957
D16Bwg1494e
1435233_at
3.396
BM234716
Ncoa2

1428600_at
3.49
AK014241
3110068G20Rik
1424199_at
3.396
AW537349
Seh1l

1448745_s_at
3.487
NM_008508
Lor
1438039_at
3.396
BE630599
Hectd1

1434603_at
3.485
BG074645
Thrap2
1455102_at
3.393
BB213860
D330037H05Rik

1419522_at
3.484
NM_026021
Zmynd19
1435549_at
3.392
BI685685
Trpm4

1444363_at
3.477
BB269445

1416013_at
3.392
NM_011116
Pld3

1423978_at
3.476
BC025837
Sbk1
1438272_at
3.391
BB315073
Csmd3

1436013_at
3.476
AI852434
C230098I05Rik
1453485_s_at
3.391
AK016897
1110005A03Rik

1433558_at
3.476
BM231046
Dab2ip
1428924_at
3.39
AU016306
Mocs3

1424320_a_at
3.474
BC008598
Traf7
1436260_at
3.387
BB131085


1434025_at
3.469
BG069607

1436354_at
3.386
BE992423
Dzip1l

1438421_at
3.469
BI134275
Pyrl1
1436763_a_at
3.386
A1267126
Klf9

1429588_at
3.468
AA509870
2810474O19Rik
1421662_a_at
3.384
NM_030254
Tusc3

1450436_s_at
3.466
AI664344
Dnajb5
1421037_at
3.384
BG070037
Npas2

1416918_at
3.465
NM_016747
Dlgh3
1438662_at
3.382
AW123234
LOC433810

1429618_at
3.465
BM119209
Cyld
1431076_at
3.382
BG976853
Add2

1449843_at
3.465
BG071333
St8sia2
1456863_at
3.381
AI385584
Epha4

1455265_a_at
3.464
BB100249
Rgs16
1421399_at
3.38
NM_016889
Insm1

1450863_a_at
3.462
BQ174703
Dcamkl1
1452292_at
3.379
AV271093
Ap2b1

1417794_at
3.461
NM_019831
Zfp261
1425132_at
3.377
AF448840
Neto1

1423583_at
3.46
AK005041
Fem1a
1437231_at
3.374
AV246497
Slitrk6

1457729_at
3.458
AW492805

1423777_at
3.374
AK006800
Usp20

1428585_at
3.456
BE853286
Actn1
1451751_at
3.372
AF335325
Ddit4l

1439106_at
3.371
AV320128
Zfp462
1447301_at
3.338
BG228875
Gm258

1421027_a_at
3.37
AI595932
Mef2c
1417890_at
3.336
NM_020271
Pdxp

1436085_at
3.37
BB361974
Zbtb34
1437918_at
3.335
AV374644
4930539E08Rik

1434819_at
3.369
BB709312
St6gal2
1434228_at
3.334
AV255921
Ppm2c

1441572_at
3.369
BB264612
C030036D22Rik
1432091_a_at
3.333
AK007455
Rutbc3

1453200_at
3.368
AK013909
Rai1
1417375_at
3.332
AW491660
Tuba4

1418181_at
3.366
AK014601
Ptp4a3
1442093_at
3.331
AU040368


1448141_at
3.364
NM_029101
1110014J01Rik
1451533_at
3.328
BC022687
BC022687

1452647_a_at
3.361
AK011199
Dph2
1427212_at
3.328
BC027377
Mapkap1

1455670_at
3.36
BQ175884
Rbms3
1458967_at
3.326
BM939341


1423367_at
3.36
AK004683
Wnt7a
1449063_at
3.326
BC009024
Sec22l1

1423832_at
3.359
BB756794
Prkag2
1455029_at
3.325
BB342219
Kif21a

1453260_a_at
3.355
AK010380
Ppp2r2a
1453268_at
3.322
AV224102
5730409G07Rik

1436396_at
3.355
AV291191
D430033N04Rik
1422594_at
3.318
BC004090
5730470L24Rik

1419879_s_at
3.354
AA960166
Trim25
1455289_at
3.317
BQ174304
Ankrd13b

1421943_at
3.351
M92420
Tgfa
1423344_at
3.316
AK010968
Epor

1415753_at
3.35
BC005632
D10Bwg1364e
1426416_a_at
3.316
AV216410
Yipf4

1434476_at
3.349
BM119896
Mect1
1438434_at
3.313
AV349116
Arhgap11a

1418174_at
3.347
BC018323
Dbp
1456760_at
3.311
BB342676


1451888_a_at
3.341
AB025413
Odz4
1451207_at
3.31
BC023022
Cbara1



ProbeSymbol = Affymetrix probe identification corresponding to gene symbol;

FEN = fold enrichment when compared to reference sample;

Genbank = Genbank identification;

GeneSymbol = official gene symbol.



















































































































































































































































































































































































































































































































































TABLE 19



Top 1,000 brain stem cholinergic motor neuron (Chat) translated

mRNAs enriched when compared to a reference sample

ProbeSymbol = Affymetrix probe identification corresponding to gene symbol; FEN = fold enrichment when

compared to reference sample; Genbank = Genbank identification; GeneSymbol = official gene symbol.










ProbeSymbol
FEN
Genbank
GeneSymbol
ProbeSymbol
FEN
Genbank
GeneSymbol












1422203_at
1618.00
NM_021712
Slc18a3
1416762_at
52.76
NM_009112
S100a10

1422639_at
1463.00
NM_054084
Calcb
1448182_a_at
51.25
NM_009846
Cd24a

1422530_at
385.40
NM_013639
Prph1
1436520_at
50.88
BB378317
AI450948

1452004_at
363.20
AF330212
Calca
1417013_at
50.33
AF250139
Hspb8

1417788_at
192.50
NM_011430
Sncg
1455439_a_at
48.08
AI642438
Lgals1

1452243_at
190.40
BB282273
Kcnj14
1427357_at
46.95
AK008793
Cda

1438042_at
177.90
AV332957
Shox2
1418787_at
45.08
NM_010776
Mbl2

1426511_at
149.60
AK004703
Susd2
1453351_at
44.45
AK020409
Tbx20

1455907_x_at
124.40
BB059017
Phox2b
1427351_s_at
43.40
BB226392
Igh-6

1438967_x_at
120.00
AV259665
Amhr2
1421359_at
41.03
NM_009050
Ret

1436461_at
115.40
BQ175922
G3bp
″AFFX-r2-




1416034_at
108.50
NM_009846
Cd24a
Bs-dap-3_at″
40.73
AFFX-r2-Bs-


1433578_at
103.50
BE824538
E130304D01


dap-3


1422644_at
98.31
NM_015825
Sh3bgr
1457021_x_at
39.81
AV209206
Amhr2

1451440_at
90.54
AF311699
Chodl
1449106_at
39.51
NM_008161
Gpx3

1420388_at
86.56
NM_008939
Prss12
1453127_at
38.86
AK009235
Ppm1j

1439609_at
85.66
BB536410

1423856_at
38.80
BC003896
Popdc3

1425964_x_at
85.58
U03561
Hspb1
1443906_at
38.28
BE686894
Daf1

1449254_at
83.13
NM_009263
Spp1
1449271_a_at
38.26
NM_019487
Hebp2

1443372_at
80.13
BB417900
Chat
1436239_at
37.11
BB328706
Slc5a5

1455893_at
78.48
BG067392
Rspo2
1448816_at
35.41
NM_008968
Ptgis

1419200_at
74.35
NM_022007
Fxyd7
1427989_at
35.01
AF503863
Amhr2

1421799_at
69.66
NM_011910
Uts2
1423557_at
34.83
BF537076
Ifngr2

1460668_at
62.98
NM_010253
Gal
1436359_at
34.72
BF471533
Ret

1422943_a_at
61.77
NM_013560
Hspb1
1423693_at
34.64
BC011218
Ela1

1454660_at
59.01
BB367590
1100001E04Rik
1419091_a_at
34.50
NM_007585
Anxa2

1419573_a_at
58.02
NM_008495
Lgals1
1415983_at
34.34
NM_008879
Lcp1

1418917_at
52.82
NM_019487
Hebp2
1418517_at
34.07
NM_008393
Irx3

1437137_at
33.87
AV280875
6430550H21Rik
1422825_at
20.10
NM_013732
MGI:1351330

″AFFX-



1421223_a_at
20.05
NM_013471
Anxa4

DapX-3_at″
31.89
AFFX-DapX-3

1420984_at
19.96
AF114437
Pctp

1437502_x_at
31.73
BB560574
Cd24a
1439015_at
19.88
AV221299
Gfra1

1454171_x_at
31.57
AK020608
9530053H05Rik
1428664_at
19.81
AK018599
Yip

1421428_at
31.11
NM_022025
Slc5a7
1439086_at
19.60
BB278168
A930009L07Rik

1418762_at
30.97
NM_010016
Daf1
1439885_at
19.35
AA051236
Hoxc5

1448291_at
30.74
NM_013599
Mmp9
1424531_a_at
19.22
BC010807
Tcea3

1446681_at
30.58
AV327329
BB086117
1424176_a_at
19.16
BE628614
Anxa4

1436503_at
30.52
BF302511
BC048546
1438444_at
18.99
AV381832
A230091H23Rik

1448160_at
30.41
NM_008879
Lcp1
1418176_at
18.97
AV290079
Vdr

1453855_at
30.02
BG295762
1810057P16Rik
1423427_at
18.96
AI323434
Adcyap1

1423947_at
29.93
BC024615
1110008P14Rik
1450869_at
18.84
AI649186
Fgf1

1428536_at
29.46
AK015001
Kcng4
1452968_at
18.50
AK003674
Cthrc1

1422586_at
28.71
NM_021306
Ecel1
1434779_at
18.48
BQ175551
Cbln2

1456532_at
28.49
BB428671
Pdgfd
1432198_at
18.14
AK018172


1418352_at
28.42
BC012682
Hsd17b2
1427975_at
18.09
AK008807
2210403B10Rik

1421862_a_at
28.31
AK018783
Vamp1
1416298_at
17.83
NM_013599
Mmp9

1416357_a_at
28.06
NM_023061
Mcam
1423281_at
17.70
BM115022
Stmn2

1418415_at
27.45
NM_008268
Hoxb5
1453638_at
17.69
AK018520
9030420J04Rik

1423560_at
27.34
AI838010
Nell2
1449545_at
17.67
NM_008005
Fgf18

1422860_at
26.84
NM_024435
Nts
1451814_a_at
17.61
AF061972
Htatip2

1452913_at
26.70
AV337888
Pcp4l1
1453593_at
17.42
BG066866
1700110N18Rik

1450610_at
26.27
NM_021290
Ucn
1447791_s_at
17.37
AV230778
Gna14

1434442_at
25.66
BB667844
D5Ertd593e
1429154_at
17.30
BG069340
Slc35f2

″AFFX-r2-



1437762_at
17.18
BB130995
Rab39

Bs-dap-M_at″
24.43
AFFX-r2-Bs-

1430596_s_at
17.13
BG066866
1700110N18Rik



dap-M

1426063_a_at
17.06
U10551
Gem

1455919_at
24.09
AV330588

1422452_at
16.97
NM_013863
Bag3

1441778_at
23.92
BB171354
Adcyap1
1435790_at
16.91
BG864960
Olfm2

1444165_at
23.75
BB530994
E030012M19Ri
1450723_at
16.81
BQ176915
Isl1




k
1417960_at
16.61
NM_007755
Cpeb1

1452010_at
23.39
AF472588
Chrna3
1457048_at
16.38
BB526932
Gpr103

1451515_s_at
23.24
BC010799
Glyat
1440677_at
16.34
BB308744
LOC245305

1424900_at
22.65
BC025599
Slc29a4
1416713_at
16.34
NM_026481
2700055K07Rik

″AFFX-



1460255_at
16.17
NM_033622
Tnfsf13b

DapX-M_at″
22.56
AFFX-DapX-

1422852_at
16.02
NM_019686
Cib2



M

1420256_x_at
15.90
AV354142


1422635_at
22.40
NM_009599
Ache
1439578_at
15.80
BB713457
Lsm11

1435184_at
22.18
BG066982
B430320C24Rik
1455642_a_at
15.79
AI844703
Tspan17

1445281_a_at
21.58
BB309504
B230311B06Rik
1418901_at
15.77
NM_009883
Cebpb

1417312_at
21.31
AK004853
Dkk3
1428544_at
15.64
BE689422
0610007L01Rik

1436141_at
21.14
BM934616
2610510L01Rik
1456247_x_at
15.57
AV166926
Plp2

1436332_at
20.96
BB755506
Hspb6
1424249_a_at
15.48
BC024535
Arhgap9

″AFFX-r2-



1459750_s_at
15.27
AU015577
Gpr123

Bs-thr-
20.94
AFFX-r2-Bs-

1452246_at
15.22
U58888
Ostf1

3_s_at″

thr-3

1424927_at
15.10
BC025083
Glipr1

1460299_at
20.73
NM_019944
Hlxb9
1436622_at
15.02
AW492241
Iqsec2

1449899_at
20.42
NM_130455
Grin3b
1426937_at
15.01
AK018128
6330406I15Rik

1420385_at
20.25
NM_008137
Gna14
1459890_s_at
14.98
C79326
1110008P14Rik

1427747_a_at
20.23
X14607
Lcn2
1422118_at
14.92
NM_023485
Sync

1442608_at
20.21
AV071586
E030012M19Ri
1417956 at
14.86
NM_007702
Cidea




k
1425245_a_at
14.81
BC019741
Rgs11

″AFFX-



1429696_at
14.81
BE946247
Gpr123

ThrX-3 at″
20.17
AFFX-ThrX-3

1422720_at
14.68
BQ176915
Isl1

1417228_at
20.11
AF084459
Capn1
1427900_at
14.53
BC028795
Pip5kl1

1424443_at
14.46
AV378394
Tm6sf1
1453591_at
12.38
AK017626
5730437N04Rik

1449155_at
14.40
NM_026190
Polr3g
1457008_at
12.36
AW456706
Chrnb4

1449571_at
14.38
M59811
Trhr
1434493_at
12.35
BG092222
1810022K09Rik

1451232_at
14.34
U89772
Cd151
1449429_at
12.31
NM_016863
Fkbp1b

1416935_at
14.31
NM_011706
Trpv2
1429732_at
12.24
AK016334
Ppp1r1c

1456925_at
14.22
AW490413
P2rxl1
1437914_at
12.20
BB104097
E2f6

1450791_at
14.19
NM_008726
Nppb
1423426_at
12.19
BB018522
1300012G16Rik

1433626_at
14.17
BB826296
Plscr4
1450885_at
12.12
BB446076
Dffa

1429385_at
14.17
BI082535
Wdr68
1415978_at
12.09
NM_023279
Tubb3

1459300_at
14.14
BB226459

1423136_at
12.04
AI649186
Fgf1

1448259_at
14.02
BI452727
Fstl1
1427854_x_at
12.02
AF047377
Hspb1

1459921_at
13.98
BQ042959
Nxph1
1456468_x_at
12.00
BB189996
1110012N22Rik

1427853_a_at
13.97
AF047377
Hspb1
1416656_at
11.95
NM_033444
Clic1

1424692_at
13.85
BC004753
2810055F11Rik
1428751_at
11.95
AK005771
Pacrg

1449161_at
13.83
NM_007902
Edn2
1448669_at
11.94
AK004853
Dkk3

1444060_at
13.80
BB497990
Bcas3
1418571_at
11.94
NM_013749
Tnfrsf12a

1451346_at
13.74
BG075139
Mtap
1424487_x_at
11.93
BB284199
Txnrd1

1457779_at
13.64
AV013305
1110046J04Rik
1426606_at
11.88
BB426194
Crtac1

1449368_at
13.64
NM_007833
Dcn
1429953_at
11.85
AK008705
2210011C24Rik

1453111_a_at
13.61
AK019396
D11Ertd333e
1429934_at
11.81
AK015678
4930502E18Rik

1455239_at
13.57
BM116861
6330512M04Rik
1416431_at
11.67
NM_026473
Tubb6

1447493_at
13.55
AI413840
A530088H08Rik
1438763_at
11.64
BB016751


1415832_at
13.54
NM_007429
Agtr2
1448660_at
11.58
NM_008113
Arhgdig

1452451_at
13.53
AF240460
MGI:1929597
1418780_at
11.52
NM_018887
Cyp39a1

1424680_at
13.50
BC025893
BB146404
1441957_x_at
11.51
AV216663
2410076I21Rik

1419810_x_at
13.50
AU043488
Arhgap9
1452508_x_at
11.50
BC021465
A230083G16Rik

1449130_at
13.50
NM_007639
Cd1d1
1442180_at
11.49
BB382040
Dleu7

1449832_at
13.48
NM_028570
1700091H14Rik
1443684_at
11.48
BE956557


1418610_at
13.47
NM_080853
Slc17a6
1418649_at
11.39
BB284358
Egln3

1425051_at
13.46
AK010892
Isoc1
1453755_at
11.38
BM951302
Lsm11

1421973_at
13.44
BE534815
Gfra1
1426221_at
11.37
BC004727
Loh11cr2a

1416080_at
13.41
NM_009614
Adam15
1442631_at
11.34
BM935306


1427512_a_at
13.32
X84014
Lama3
1451322_at
11.27
BC024580
2310016A09Rik

1451636_at
13.31
BC022726
Ankrd33
1436238_at
11.23
AI841179
Lgi3

1422545_at
13.28
NM_009324
Tbx2
1434341_x_at
11.20
BF681728
1110020P15Rik

1434340_at
13.26
BF681728

1429231_at
11.18
AK015078


1426540_at
13.21
BF168366
2310067E08Rik
1451956_a_at
11.15
AF226605
Oprs1

1417291_at
13.20
L26349
Tnfrsf1a
1450230_at
11.10
NM_138582
D17H6S56E-3

1428384_at
13.18
AK003950
D4Bwg0951e
1456251_x_at
11.08
BB132602
Bzrp

1456596_at
13.13
BB093996
6430550H21Rik
1417943_at
11.06
NM_010317
Gng4

1456746_a_at
13.05
BB334959
Cd9912
1451413_at
11.03
AB026997
Cast

1444790_at
13.04
AV048499
1810005K13Rik
1427004_at
10.98
BB311718
Fbxo2

1424246_a_at
12.98
BC003808
Tes
1418268_at
10.98
NM_013561
Htr3a

1419555_at
12.97
BC012424
Elf5
1456642_x_at
10.94
AV295650
S100a10

1439905_at
12.93
BE948639

1421483_at
10.91
NM_010712
Lhx4

1422245_a_at
12.91
U63408
Mrvi1
1430539_at
10.91
BB469133
1810057P16Rik

1417014_at
12.91
AF250139
Hspb8
1443505_at
10.87
BE651535
A630076J17Rik

1424093_x at
12.86
U89772
Cd151
1437139_at
10.84
AV344554
Glra1

1452103_at
12.77
BI456906
2600005N12Rik
1425341_at
10.81
BF467278
Kcnk3

1422870_at
12.60
NM_013553
Hoxc4
1426255_at
10.77
M20480
Nefl

1418471_at
12.54
NM_008827
Pgf
1425326_at
10.70
BI456232
Acly

1442865_at
12.50
BB361845
MGI:3580254
1454891_at
10.69
BM214378
Cds2

1435813_at
12.44
BM212838
Mypn
1427375_at
10.68
BB198308
Rg9mtd2

1438948_x_at
12.43
AV101079
Bzrp
1426926_at
10.66
AW546508
Plcg2

1429007_at
12.43
AK003377
Slc35b2
1420559_a_at
10.64
NM_013665
Shox2

1455931_at
12.42
BB460687
Chrna3
1422185_a_at
10.62
NM_029787
Cyb5r3

1436528_at
10.60
AI842353
Kazald1
1426444_at
9.49
BB233055
Rhbdl7

1417389_at
10.56
NM_016696
Gpc1
AFFX-r2-Bs-
9.47
AFFX-r2-Bs-


1445475_at
10.56
BB003750
Pak6
thr-M_s_at

thr-M


1445124_at
10.54
BB193580
A930009L07Rik
1422481_at
9.47
NM_008473
Krt2-1

1418916_a_at
10.54
NM_029269
Spp2
1424976_at
9.43
BC021307
Rhov

1448883_at
10.53
NM_011175
Lgmn
1450001_a_at
9.40
NM_023649
Ush1c

1452806_at
10.51
AK005271
1500016010Rik
1460378_a_at
9.40
BC010465
Tes

1427539_a_at
10.50
BC013559
Zwint
1441481_at
9.39
AV262974
Mfap3l

1455162_at
10.50
BI147002
4922503N01Rik
1428190_at
9.37
AK005070
Slc25a1

1434976_x_at
10.49
AV216412
Eif4ebp1
1457696_at
9.36
AI835553
Rilp

1424847_at
10.46
M35131
Nefh
1439186_at
9.34
BB022463
E130319B15Rik

1450389_s_at
10.45
NM_008846
Pip5k1a
1429775_a_at
9.33
AK009736
Tm7sf1

1456524_at
10.44
AV318914
Nrg1
1424580_at
9.33
AW822833
Slc35a3

1421391_at
10.44
NM_009511
Vipr2
1437263_at
9.32
BB138441
A730089K16Rik

1449397_at
10.43
NM_134032
Hoxb2
1459888_at
9.31
AI429562
LOC545261

1417988_at
10.42
NM_009049
Resp18
1460351_at
9.24
BC021916
S100a11

1457281_at
10.41
BB224547
4930461P20Rik
1453341_a_at
9.22
AK008510
4930425N13Rik

1451680_at
10.36
BC011325
Srxn1
1425050_at
9.20
AK010892
Isoc1

1417473_a_at
10.35
NM_026494
Ppcs
1419455_at
9.18
NM_008349
Il10rb

1456187_at
10.33
BB278836
Slc7a14
1423720_a_at
9.18
BC005549
Sara1

1436035_at
10.31
AV225683
3830431G21Rik
1450444_a_at
9.16
NM_013839
Nr1h3

1424048_a_at
10.31
BC024618
Cyb5r1
1434092_at
9.14
AW121498
Nos3as

1421781_at
10.29
NM_009476
Upk2
1437670_x_at
9.13
BB113673
Cd151

1416695_at
10.28
BC002055
Bzrp
1452936_at
9.08
AV341285
Crtac1

1416750_at
10.27
NM_011014
Oprs1
1417133_at
9.06
NM_008885
Pmp22

1429348_at
10.27
AK004119
Sema3c
1423425_at
9.03
BB018522
1300012G16Rik

1448781_at
10.23
NM_008667
Nab1
1447773_x_at
9.02
BB308792
6330409D20Rik

1418403_at
10.22
NM_009616
Adam19
1449426_a_at
8.98
NM_011922
Anxa10

1441280_at
10.19
BF322939
Kcnk12
1429684_at
8.97
BG094398
5830472M02Rik

1431597_a_at
10.16
AK020731
Nrip3
1423561_at
8.94
AI838010
Nell2

1425963_at
10.16
AF419324
Cabp7
1418710_at
8.94
NM_007652
Cd59a

1416111_at
10.16
NM_009856
Cd83
1460174_at
8.92
NM_021428
Dexi

1427940_s_at
10.13
BB046347
Mycbp
1449848_at
8.91
NM_008137
Gna14

1451345_at
10.12
BG075139
Mtap
1460191_at
8.91
NM_019661
0610042I15Rik

1452449_at
10.10
AI006067
F830020C16Rik
1439006_x_at
8.89
BB093996
6430550H21Rik

1456747_x_at
10.05
BB334959
Cd99l2
1449619_s_at
8.88
AU043488
Arhgap9

1435815_at
10.03
AW541598
Ldoc1
1416592_at
8.88
AF276917
Glrx1

1427951_s_at
10.02
BG141739
Ccdc28a
1451099_at
8.86
BC011482
Mbc2

1432224_at
10.02
AK019510
4831407H17Rik
1420664_s_at
8.85
NM_011171
Procr

1432211_a_at
9.97
AK018482
Fbxo9
1460242_at
8.85
NM_010016
Daf1

1455736_at
9.97
AI326984
Mybpc2
1416168_at
8.83
NM_011340
Serpinf1

1436295_at
9.95
BB803143
Hcrtr1
1460379_at
8.82
AV307188
Hoxb4

1449079_s_at
9.90
NM_018784
St3gal6
1415919_at
8.82
NM_008721
Npdc1

1434055_at
9.83
BB048942
Galnt9
1446729_at
8.81
BB179083
Disp2

1455947_at
9.83
BB667208
9630025C22
1448722_s_at
8.81
NM_026494
Ppcs

1423966_at
9.78
BB038546
Cd99l2
1434528_at
8.79
AV256613
Aard

1419100_at
9.78
NM_009252
Serpina3n
1431160_x_at
8.78
AW543121
6030426L16Rik

1416871_at
9.77
NM_007403
Adam8
1418345_at
8.77
NM_023517
″Tnfsf13 ;

1456229_at
9.70
BG073383
Hoxb3
Tnfsf12-




1426595_at
9.69
BB011871
Slc18a1
tnfsf13″




1421195_at
9.69
BC020534
Cckar
1421834_at
8.76
NM_008846
Pip5k1a

1430521_s_at
9.65
AW548480
Cpne8
1425052_at
8.74
AK010892
2610034N03Rik

1430114_at
9.62
AK017323
5430420C16Rik
1430577_at
8.68
BE686390
2410007P03Rik

1457429_s_at
9.61
BB549174
Gm106
1441363_at
8.67
BQ174855
LOC546381

1459665_s_at
9.57
BB800078
Mrvil
1416435_at
8.66
NM_010736
Ltbr

1418713_at
9.57
NM_025273
Pcbd1
1416148_at
8.66
BC019120
Laptm4b

1451210_at
8.65
BC010332
Ppap2c
1415818_at
7.88
NM_013472
Anxa6

1449928_at
8.65
NM_025975
Tcte1l
1437040_at
7.85
AV280878
Etnk2

1422520_at
8.64
NM_008691
Nef3
1418107_at
7.84
NM_009326
Tcea2

1436948_a_at
8.62
BB520013
6430550H21Rik
1437648_at
7.84
BB541022
Pcyt1b

1434046_at
8.58
BG961961
AA467197
1433600_at
7.83
BB262415


1435972_at
8.57
BB148748
Cast
1426542_at
7.82
BF168366
2310067E08Rik

1460702_at
8.56
AK007514
1810015M01Rik
1441049_at
7.81
AV328356
Kcna6

1456085_x_at
8.55
AV169215
Cd151
1418686_at
7.80
NM_033541
Oas1c

1427340_at
8.50
BB274058
Rgs6
1449921_s_at
7.80
NM_009947
Cpne6

1420566_at
8.46
NM_025367

1440531_at
7.80
BB333100
Rbm11

1429246_a_at
8.45
AK013026
Anxa6
1416221_at
7.80
BI452727
Fstl1

1426319_at
8.44
AF335583
Pdgfd
1421606_a_at
7.79
NM_013873
Sult4a1

1438055_at
8.43
BB035017
Rarres1
1418572_x_at
7.79
NM_013749
Tnfrsf12a

1439807_at
8.43
BB816169
Tmem74
1452450_at
7.79
BG277213
Atp8b2

1453067_at
8.43
AU043467
2610040C18Rik
1439959_at
7.77
AW488620
Fgfl1

1424129_at
8.41
BC024891
Mfsd1
1431105_a_at
7.77
AK012413
Tmem33

1425828_at
8.40
AF357883
Nkx6-1
1445424_at
7.76
BB082380


1448429_at
8.38
NM_013755
Gyg1
1415856_at
7.75
BG064842
Emb

1456778_at
8.34
BB409477
Nrp2
1427182_s_at
7.73
BQ032363
D18Ertd653e

1430648_at
8.32
AK013326
Scn2b
1441902_x_at
7.73
BB264298


AFFX-ThrX-
8.31
AFFX-ThrX-

1441284_at
7.72
BB283819


M_at

M

1451707_s_at
7.72
BC011108
Slc41a3

1452399_at
8.29
BB274058
Rgs6
1439603_at
7.72
AW125555


1458932_at
8.24
BB639093
Pex2
1443198_at
7.68
BB107751


1446838_at
8.20
BB292625
Atad1
1434494_at
7.68
BG071693
Zar1

1460377_a_at
8.18
BC005722
Tmem8
1426672_at
7.64
BB327301
Tmem16k

1423345_at
8.18
AV286991
Degs1
1420654_a_at
7.64
NM_028803
Gbe1

1448693_at
8.17
NM_024472
BC002216
1448260_at
7.64
NM_011670
Uchl1

1430999_a_at
8.15
BM932452
Scoc
″AFFX-r2-




1430778_a_at
8.15
AK020876
Nubp1
Bs-phe-5_at″
7.63
AFFX-r2-Bs-


AFFX-LysX-
8.14
AFFX-LysX-3



phe-5


3_at



1417038_at
7.63
NM_017380


1435134_at
8.12
AV369935
Aadacl1
1434665_at
7.63
AV052058
Aga

1451666_at
8.12
BI456232
Acly
1417430_at
7.62
NM_007672
Cdr2

1449961_at
8.10
NM_011286
Rph3a
1441551_at
7.60
BB196537
Mypn

1436509_at
8.09
BB000110
2410014A08Rik
1420731_a_at
7.60
NM_007792
Csrp2

1438717_a_at
8.07
BG070848
Osbpl6
1423087_a_at
7.60
BB453951
1110002E23Rik

1432319_at
8.07
AK007004
1700085C21Rik
1430780_a_at
7.60
BI739353
Pmm1

1427708_a_at
8.06
L22989
Nf2
1431086_s_at
7.60
AA389937
Pcmt1

1453523_at
8.03
AK020698
A030006P16Rik
1417780_at
7.59
BB006809
Lass4

1428400_at
8.03
BB066985
2200002K05Rik
1455783_at
7.58
AV361804


1429204_at
8.03
AK013788
2900075A18Rik
1426622_a_at
7.57
BB150720
Qpct

1429203_at
7.99
AK010725
2410076121Rik
″AFFX-r2-




1460239_at
7.97
BB807707
Tspan13
Bs-dap-5_at″
7.56
AFFX-r2-Bs-


1453232_at
7.97
AK006582
Calr3


dap-5


1442600_at
7.97
BB456595

1443745_s_at
7.55
AV020965
Dmp1

1437107_at
7.95
AV220161
Rab6b
1449139_at
7.54
NM_025509
2310008M10Rik

1429266_at
7.95
AK020670
Tmhs
1430514_a_at
7.52
AK002762
2410026K10Rik

1452362_at
7.94
BB834440
Trim16
1440071_at
7.52
BB367260
Baiap1

1419473_a_at
7.94
NM_031161
Cck
1418173_at
7.50
NM_133730
4631426H08Rik

1444204_at
7.93
BE687976
Hoxb2
1437217_at
7.50
BM225135
Ankrd6

1429087_at
7.91
AK005245
1110054O05Rik
1425573_a_at
7.48
BE943736
Ddef1

1424050_s_at
7.91
M33760
Fgfr1
1449491_at
7.47
NM_130859
Card10

1424518_at
7.90
BC020489
″2310016F22Rik
1421321_a_at
7.47
NM_019671
Net1




;
1451828_a_at
7.47
AB033886
Acsl4

LOC223672″



1420693_at
7.47
NM_010867
Myom1

1440413_at
7.46
BB800596
A830006F12Rik
1448944_at
6.96
AK011144
Nrp1

1449189_at
7.45
NM_030252
BC003266
1424178_at
6.94
BC014728
Tmem38a

1418175_at
7.44
AV290079
Vdr
1454604_s_at
6.93
BB072896
Tspan12

1417507_at
7.43
BC006732
Cyb561
1420386_at
6.93
NM_028112
Seh1l

1417969_at
7.42
NM_133765
Fbxo31
1455622_at
6.89
BB461988
Podxl2

1425329_a_at
7.42
AF332060
Cyb5r3
1418752_at
6.89
NM_007436
Aldh3a1

1450731_s_at
7.39
BG972377
Tnfrsf21
1443812_x_at
6.89
BB319311
Prkg1

1416046_a_at
7.39
BM054266
Fuca2
″AFFX-




1422583_at
7.37
NM_023537
Rab3b
DapX-5_at″
6.88
AFFX-DapX-5


1417902_at
7.37
NM_054087
Slc19a2
1443746_x_at
6.86
AV020965
Dmp1

1426543_x_at
7.36
BF168366
2310067E08Rik
1418084_at
6.85
AK011144
Nrp1

1449983_a_at
7.35
NM_020282
Nqo2
1443664_s_at
6.85
AW123724
Cd164l2

1424560_at
7.35
U87814
Pstpip1
1451386_at
6.84
BC027279
Blvrb

1431255_at
7.34
AI324734
Calr3
1429783_at
6.83
AK009464
Pdlim5

1417539_at
7.34
NM_011895
Slc35a1
1452545_a_at
6.80
U37029
Itgb1

1424882_a_at
7.34
BC011230
2510015F01Rik
1450440_at
6.79
BE534815
Gfra1

1418206_at
7.34
NM_022324
Sdf2l1
1460409_at
6.79
AI987925
Cpt1a

1436339_at
7.32
BE627374
1810058I24Rik
1418474_at
6.79
NM_033146
1500005A01Rik

1418248_at
7.27
NM_013463
Gla
1430726_at
6.78
AV331288
Rassf8

1449012_s_at
7.25
BC027164
Fndc4
1438623_x_at
6.78
AV038578
Rbx1

1435523_s_at
7.25
BB779140
2700089E24Rik
1433796_at
6.77
BI734389
2310067E08Rik

1431946_a_at
7.24
AK013520
Apba2bp
1455085_at
6.76
BI526033
1700086L19Rik

1435575_at
7.21
AW536884
Kntc1
1427548_a_at
6.75
U53455
Clns1a

1427919_at
7.19
BC028307
Srpx2
1442015_at
6.75
BG070940


1419728_at
7.18
NM_009141
Cxcl5
1422854 _at
6.74
BB753533
Shc1

1418129_at
7.18
BG295389
Dhcr24
1416593_at
6.72
AF276917
Glrx1

1455025_at
7.14
AV103696
Paqr9
1436902_x_at
6.70
BB096368
Tmsb10

1450500_at
7.13
AW012751
Uhmk1
1459717_at
6.70
AW045505


1418478_at
7.13
NM_057173
Lmo1
1436861_at
6.68
BG069762
Il7

1434256_s_at
7.13
BM214378
Cds2
1418451_at
6.68
BB522409
Gng2

1448549_a_at
7.12
NM_007875
Dpagt1
1427196_at
6.68
BG074348
Wnk4

1455775_at
7.12
AW988196

1450138_a_at
6.67
NM_009254
Serpinb6a

1429712_at
7.12
AK005093
Etohi1
1448772_at
6.65
BG868960
Ube2a

1445564_at
7.11
BE688513

1418452_at
6.65
BB522409
Gng2

1421127_at
7.10
NM_025339
Tmem42
1444178_at
6.64
BB609792
LOC433237

1430631_at
7.10
AK016942
Ppm1f
1455427_at
6.64
AV269710
Angpt4

1416914_s_at
7.10
NM_023166
Mtvr2
1423954_at
6.64
K02782
C3

1429300_at
7.09
AK010867
Ankrd9
1423280_at
6.64
BM115022
Stmn2

1425440_x_at
7.09
BC011108
Slc41a3
1460375_at
6.62
BC019418
0610038D11Rik

1422158_at
7.08
NM_022319
Clstn2
1419398_a_at
6.61
NM_007874
Dp1

1418371_at
7.07
AY029255
6720463E02Rik
1419383_at
6.61
NM_009115


1454893_at
7.06
BB765852
1110013L07Rik
1454227_at
6.61
AK015389
Htatip2

1418187_at
7.04
AF146523
Ramp2
1438007_at
6.60
BB432758
AI851790

1417807_at
7.04
NM_027356
2700038NO3Rik
1419518_at
6.59
NM_017379
Tuba8

1424486_a_at
7.03
BB284199
Txnrd1
1431446_at
6.57
AK002366
0610009B14Rik

1424109_a_at
7.02
BC024663
Glo1
1428139_at
6.56
AK015988
4930538D17Rik

1452807_s_at
7.02
AK005271
1500016O10Rik
1436150_at
6.54
BB391675
2310028H24Rik

1433787_at
7.01
AI841091
B230343H07Rik
1450486_a_at
6.54
X91813
Oprl1

1433793_s_at
7.01
AW491344
Nrip2
1416673_at
6.53
NM_019517
Bace2

1445437_at
7.00
BE948587
2310015A05Rik
1419550_a_at
6.52
BG919998
Stk39

1456857_at
6.99
BB782615

1437043_a_at
6.52
AV012400
1110012M11Rik

1451427_a_at
6.99
BC024610
Egfl7
1450787_at
6.48
NM_016691
Clcn5

1418890_a_at
6.98
BB349707
Rab3d
1420772_a_at
6.47
NM_010286
Tsc22d3

1440248_at
6.97
BB238478
Casc4
″AFFX-




1460466_at
6.97
BB824055
1700047117Rik
PheX-M_at″
6.46
AFFX-PheX-


1439407_x at
6.96
AV212626
Tagln2


M


1441281_s_at
6.45
AU024536
Ninj1
1418104_at
6.09
NM_020610
Nrip3

1446218_at
6.42
BB306329
Ppfia2
1421034_a_at
6.09
NM_010557
Il4ra

1449433_at
6.42
NM_033321
P2rx5
1431241_at
6.08
AA692147
Chchd3

1422597_at
6.41
NM_008609
Mmp15
1429522_at
6.07
BB796723
Ankrd42

1417290_at
6.41
NM_029796
Lrg1
1429753_at
6.07
AK004130
Nxph4

1444291_at
6.41
BB184872
4930506D23Rik
1427773_a_at
6.07
L40934
Rabac1

1441456_at
6.40
BB335489
Mmp24
1440308_at
6.06
AV347071
0610011D08Rik

1448300_at
6.40
NM_025569
Mgst3
1424732_s_at
6.06
BC020157
3110005G23Rik

1452825_at
6.40
AK005155
5330410G16Rik
1455197_at
6.05
BE852181
Rnd1

1427956_at
6.40
BG068910
Pcgf1
1431684_at
6.05
AK016622
4933402J24Rik

1417399_at
6.38
NM_019521
Gas6
1424773_at
6.03
BC020119
1110012M11Rik

1438635_x_at
6.38
BB258476
B930041F14Rik
1417040_a_at
6.03
NM_016778
Bok

1449460_at
6.38
AF403041
Asb13
1418934_at
6.02
NM_011839
Mab21l2

1426858_at
6.37
BB253137
Inhbb
1425053_at
6.02
AK010892
Isoc1

1428495_at
6.35
BF300229
2410003K15Rik
1423321_at
6.01
BI078799
Myadm

1423951_at
6.35
BC024620
Tm2d3
1429756_at
6.01
AK016481
4931428F04Rik

141599_at
6.35
NM_009270
Sqle
″AFFX-r2-




1451426_at
6.34
AF316999
D11Lgp2e
Bs-lys-M_at″
6.01
AFFX-r2-Bs-


1417022_at
6.34
NM_007515
Slc7a3


lys-M


1422620_s_at
6.33
NM_008903
Ppap2a
1451428_x_at
6.00
BC024610
Egfl7

1423840_at
6.33
BC026206
D11Ertd99e
1460059_at
6.00
BB272732
Upp2

1429840_at
6.33
AW491495
4933439G12Rik
1420712_a_at
6.00
NM_008281
Hpn

1424356_a_at
6.32
BC024445
Metrnl
1436408_at
5.99
BE946298


1452244_at
6.31
AK018128
6330406I15Rik
1422645_at
5.98
AJ306425
Hfe

1442011_at
6.31
BB177150
A230065H16Rik
1423948_at
5.98
BC016230
Bag2

1416710_at
6.30
NM_026239
Tmem35
1429830_a_at
5.97
AK005507
Cd59a

1416410_at
6.30
NM_008776
Pafah1b3
1435332_at
5.96
BQ175864
Htr7

1437501_at
6.30
BB125794
AF529169
1421015_s_at
5.96
NM_021498
Pole3

1431359_a_at
6.29
AK003526
1110007C09Rik
″AFFX-




1450738_at
6.29
NM_016705
Kif21a
LysX-M_at″
5.94
AFFX-LysX-


1433462_a_at
6.29
BB208212
Pi4k2a


M


1454811_a_at
6.28
AV026664
Tde2
1451501_a_at
5.94
BC024375
Ghr

1424426_at
6.28
BG075139
Mtap
1454219_at
5.94
AK013295
Dnajc2

1444687_at
6.27
BB804635
C1ql2
1459669_at
5.94
AW913782
D930010J01Rik

1458438_at
6.27
AW554226
4933415L06Rik
1457076_at
5.93
BQ175967
Gm693

1426376_at
6.25
BI249075
Dp1
1448477_at
5.93
NM_021528
Chst12

1430520_at
6.25
AW548480
Cpne8
1436127_at
5.93
AI854101
Crhbp

1427202_at
6.24
AV002340
4833442J19Rik
1428823_at
5.93
AK009957


1416979_at
6.24
NM_025624
2510048O06Rik
1449473_s_at
5.91
NM_011611
Cd40

1433835_at
6.23
BE825122
Ppp3cb
1436718_at
5.91
BB274960
Nxph1

1431226_a_at
6.21
AK013203
Fndc4
″AFFX-




1458413_at
6.20
BB357976
Fbxw8
PheX-3_at″
5.91
AFFX-PheX-3


1418359_at
6.20
NM_024479
Wbscr27
1450040_at
5.90
M93954
Timp2

1452608_at
6.20
BB046347
Mycbp
1436416_x_at
5.88
BB609429
Fxc1

1422637_at
6.20
NM_018750
Rassf5
1458395_at
5.84
BB811756
B930054O08

1432271_a_at
6.19
AK012764
Dcun1d5
1426220_at
5.84
BC019457
4930471M23Rik

″AFFX-r2-



1449347_a_at
5.83
NM_021365
Xlr4b ; Xlr4a

Bs-phe-M_at″
6.16
AFFX-r2-Bs-

1427246_at
5.83
BB702366
Magi1



phe-M

1448699_at
5.82
NM_025317
Mrpl54

1417458_s_at
6.16
NM_025415
Cks2
1448852_at
5.82
NM_009060
Rgn

1417026_at
6.15
BC024693
Pfdn1
1453605_s_at
5.82
AK007017
1810060J02Rik

1417979_at
6.15
AF291655
Tnmd
1449283_a_at
5.82
BC021640
Mapk12

1417203_at
6.15
BC010592
Ethe1
1437292_at
5.81
BB431052
A330019N05Rik

1423101_at
6.14
BB279185
Paqr4
1432349_a_at
5.81
AK004279
Sync

1430098_at
6.14
AK018150
6330409D20Rik
1460150_at
5.81
BE979371
Sh2d3c

1429273_at
6.11
AK014221
Bmper
1438568_at
5.81
BB373312
Mrgpre

1417149_at
5.80
NM_011031
P4ha2
1428357_at
5.59
AK011462
2610019F03Rik

1451683_x_at
5.80
M34962
H2-D1
1419118_at
5.57
NM_021387
2900093B09Rik

1455346_at
5.80
BB477214
Masp1
1428804_at
5.57
AK017269
Mfap3l

1450661_x_at
5.79
NM_008688
Nfic
1429110_a_at
5.56
AW701004
2810405F18Rik

1444009_at
5.78
AV217868
Rassf4
1416022_at
5.55
BC002008
Fabp5

1437637_at
5.77
BE852433
Phtf2
1422965_at
5.55
NM_009642
Agtrap

1458299_s_at
5.76
BB820441
Nfkbie
1443811_at
5.55
BB319311
Prkg1

1454771_at
5.76
AW060701

1430332_a_at
5.55
AK002832
Gusb

1454800_at
5.76
BF319573
MGI:2674071
1453281_at
5.54
BB700084
Pik3cd

1424767_at
5.76
AB019618
Cdh22
1448987_at
5.54
BB728073
Acadl

1459552_at
5.75
BB404047

1439221_s_at
5.54
BB220422
Cd40

1453607_at
5.75
AV280494
Mfap3l
1435404_at
5.54
BB023743
Disp2

1418344_at
5.75
NM_021793
Tmem8
1453461_at
5.53
AK002949
Fxc1

1423211_at
5.75
AK004120
Nola3
1427373_at
5.53
BC010598
Amigo1

1416994_at
5.74
NM_133795
Ttc1
1434049_at
5.53
BB547934
Entpd3

1428377_at
5.74
AK018115
Btbd11
1452717_at
5.53
BM230959
Slc25a24

1419399_at
5.73
AW553649
Mttp
1428663_at
5.53
AK017223
5133401H06Rik

1416028_a_at
5.73
NM_008258
Hn1
1423158_at
5.52
AK008566
Gnpnat1

1427329_a_at
5.73
AI326478
Igh-6
1423686_a_at
5.52
BC016234
1110020C13Rik

1424278_a_at
5.72
BC004702
Birc5
1437462_x_at
5.52
BB484002


1454613_at
5.72
AV162270
9430041P20Rik
1424699_at
5.51
BC006583
4921511K06Rik

1438378_at
5.72
AV208934
1700013G23Rik
1457124_at
5.50
AV328224


1429743_at
5.72
BG068031
6720468P15Rik
1426298_at
5.50
AF295369
Irx2

1451690_a_at
5.71
BC024948
Pvrl4
1420554_a_at
5.49
NM_133223
Rac3

1427937_at
5.70
BB410125
″2610030H06Ri
1433667_at
5.48
BB187947
Lgi3




k ;
1430111_a_at
5.48
AK012821
Bcat1

LOC434793 ;



1416637_at
5.46
NM_009207
Slc4a2

LOC545620″



1452770_at
5.46
AK003237
Vkorc1

1452579_at
5.70
AK009021
Nifun
1439152_at
5.46
BE985292
BC052066

1417457_at
5.69
NM_025415
Cks2
1437936_at
5.45
AI428240
6330534C20Rik

1423552_at
5.69
BF658789
Leprotl1
1428379_at
5.45
BQ180367
Slc17a6

1416749_at
5.69
NM_019564
Htra1
1425780 a at
5.45
BC024352
0610041E09Rik

1455378_at
5.68
BI735497
BC057371
1416953 at
5.45
NM_010217
Ctgf

1432287_a_at
5.67
AK016927
Sntg1
1448592_at
5.45
NM_019922
Crtap

1436869_at
5.67
AV304616
Shh
1451817_at
5.44
BC011214
Suv420h1

1423653_at
5.67
BC025618
Atp1a1
1418482_at
5.44
NM_019720
Cyb561d2

1424842_a_at
5.67
BC025502
Arhgap24
1438388_at
5.44
BB078029


1436124_at
5.66
BE996519
Pcyt1b
1420113_s_at
5.43
AA409325
2410022L05Rik

1416230_at
5.66
NM_019437
Rfk
1420548_a_at
5.43
NM_023197
2310008H09Rik

1457232_at
5.66
BE946365
Fbxl21
1423286_at
5.41
AA016422
Cbln1

1428988_at
5.65
AK006128
Abcc3
1455793_at
5.41
BB766438
BC035537

1450010_at
5.65
AK012103
Hsd17b12
1435244_at
5.40
BF535947
Vav2

1449078_at
5.65
NM_018784
St3gal6
1453715_at
5.39
AK015921
Sv2c

1453752_at
5.65
BF453369
Rpl17
1446260_at
5.39
BB282393
Six6os1

1455147_at
5.64
BB177828

1421863_at
5.38
AK018783
Vamp1

1427481_a_at
5.64
BC027114
Atp1a3
1419740_at
5.37
NM_008806
Pde6b

1426918_at
5.64
BM120341
Itgb1
1428734_at
5.37
AK018169
3200002M19Rik

1435551_at
5.63
BG066491
FHOS2
1427181_at
5.37
BQ032363
D18Ertd653e

1425351_at
5.62
BC011325
Srxn1
1435109_at
5.37
AW123256
″0710007G10Ri

1455812_x_at
5.62
BB530515
Slitl2



k ;

1416781_at
5.61
NM_138602
Praf2
3010001K23




1447975_a_at
5.60
AI429562
LOC545261
Rik″




1459927_at
5.60
AA867818
4833445I07Rik
1449267_at
5.36
BC006876
3110023E09Rik

1449471_at
5.60
NM_021452
Kcnmb4
1437943_s_at
5.36
AA764430
Mea1

1418888_a_at
5.60
NM_013759
Sepx1
1448140_at
5.36
NM_134141
Ciapin1

1456231_at
5.59
BB315153
Pla2g3
1449021_at
5.36
NM_026308
Rpp21

1429212_a_at
5.36
AK005758
Lrrc51
1419254_at
5.09
BG076333
Mthfd2

1422717_at
5.35
AW554436
Acp1
1424528_at
5.09
BC023116
Cgref1

1456663_x_at
5.35
BB718785
Tm2d2
1423608_at
5.09
BI966443
Itm2a

1448472_at
5.35
AF087680
Vars2
1422559_at
5.09
AY039837
Ube2n

1438746_at
5.35
BB253959
A530058N18Rik
1435017_at
5.09
AV000569
Mel13

1425155_x_at
5.35
M21149
Csf1
1418736_at
5.08
BC003835
B3galt3

1417240_at
5.34
NM_011777
Zyx
1436250_at
5.08
BM946369
5430405G05Rik

1453558_at
5.33
AK015697
4930504H06Rik
1447683_x_at
5.08
AV118676
Mettl1

1422952_at
5.32
NM_023893
MGI:2388100
1449850_at
5.08
NM_022723
Scube1

1437950_at
5.32
BB818275
BC035537
1424403_a_at
5.07
AW494299
D5Bwg0860e

1430219_at
5.32
AK017861
Fts
1432181_s_at
5.07
AK013765
Ecgf1

1420614_at
5.31
NM_025975
Tcte1l
1429602_at
5.06
AK009888
Cd164l2

1436978_at
5.30
AV273409
Wnt9a
1450506_a_at
5.06
NM_026531
Isg20l1

1416366_at
5.30
NM_024220
Ndufc2
1450925_a_at
5.06
BB836796
Rps27l

1460030_at
5.29
BB355811
Hecw1
1460483_at
5.05
AV115271
2610034E01Rik

1436433_at
5.28
AV264828
BC049762
1430040_at
5.05
AK006867
Hspa12a

1440772_x_at
5.28
BB257157
9530077C05Rik
1460628_at
5.05
AV245208
Nme3

1425784_a_at
5.28
D78264
Olfm1
1442466_a_at
5.04
BG071019
B430315C20Rik

1451409_at
5.27
BC025858
2210021J22Rik
1436377_at
5.04
BI410102
AI428855

1436800_at
5.27
AV376318
Fstl5
1429274_at
5.04
AK009282
2310010M24Rik

1416940_at
5.27
NM_134084
Ppif
1437055_x_at
5.04
BB143944
1200003O06Rik

1451493_at
5.27
BC026372
Ndfip1
1417832_at
5.03
BB156359
Smc1l1

1451395_at
5.26
BC021492
D5Bwg0834e
1435130_at
5.03
AI131918
LOC383103

1426254_at
5.26
AF353993
Tm2d1
1418746_at
5.03
NM_019999
MGI:1930773

1436517_at
5.26
BI416101
H1fx
1460674_at
5.03
BC022922
Paqr7

1453304_s_at
5.25
BM245572
Ly6e
1454006_a_at
5.03
AK016964
Ubxd6

1423034_at
5.25
AK004219
Txnl5
1429088_at
5.03
AK007400
MGI:1925139

1432415_at
5.25
AK014050
Rab3c
1430889_a_at
5.01
AK002335
Tpmt

1426621_a_at
5.25
BB560759
Ppp2r2b
1430053_a_at
5.00
AK019142
2810409H07Rik

1432591_at
5.25
BB635017
Pappa
1447728_x_at
5.00
BB718260
Hspa9a

1416245_at
5.24
NM_025338
Aurkaip1
″AFFX-r2-




1434980_at
5.24
AV230647
Pik3r5
Bs-phe-3_at″
5.00
AFFX-r2-Bs-


1452923_at
5.24
AK007894
1810058I14Rik


phe-3


1419445_s_at
5.23
NM_009119
Sap18
1424579_at
5.00
AW822833
Slc35a3

1430869_a_at
5.23
BF460630
Habp4
1449256_a_at
4.99
BC010722
Rab11a

1430866_at
5.23
BB432681
4921537D05Rik
1423423_at
4.99
BF319868
Pdia3

1442143_at
5.22
AI463477
Tmem16d
1434549_at
4.99
BI083615
Rab11a

1417619_at
5.22
BE368753
Gadd45gip1
1449517_at
4.99
BB119571
Qpctl

1431694_a_at
5.21
AK003595
Ctnnbip1
1422034_a_at
4.99
NM_023128
Palm

1417627_a_at
5.21
NM_010717
Limk1
″AFFX-r2-




1435297_at
5.21
BI737842
Gja9
Bs-lys-3_at″
4.99
AFFX-r2-Bs-


1428753_a_at
5.21
AK019346
Dgcr6


1ys-3


1452427_s_at
5.19
BM950003
AW742319
1438251_x_at
4.98
BB559067
Htra1

1442542_at
5.18
BB363812
Eya4
1454572_at
4.98
AK013093
2810414N06Rik

1425090_s_at
5.16
BC024837
Kcnc4
1449107_at
4.97
NM_027722
Nudt4

1415857_at
5.15
BG064842
Emb
1416167_at
4.97
NM_016764
Prdx4

1430777_a_at
5.14
AK014644
Golph3
1428650_at
4.97
AK003780
Tns1

1451128_s_at
5.14
BC003427
Kif22
1458997_at
4.97
BB183491
A230106M20Ri

1431507_a_at
5.14
AK008254
Synj2bp



k

1431510_s_at
5.12
AK008567
2010110K16Rik
1426528_at
4.97
BQ176723
Nrp2

1453358_s_at
5.11
BB238321
″5830467E07Ri
1438957_x_at
4.96
BB027654
Cds2




k ;
1436512_at
4.96
BI964400
Arl7

C730024G19



1449083_at
4.96
AA067702
1810060J02Rik

Rik″



1436094_at
4.95
BF458396


1426400_a_at
5.11
BI159030
Capns1
1457558_at
4.95
AV325555
A330050F15Rik

1437249_at
5.10
BG075562
Scap1
1417916_a_at
4.95
NM_019502
Fxc1 ;




8030491N06Rik
1459858_x_at
4.79
AV354465
Crtac1

1430134_a_at
4.94
AK008774
Yars2
1456233_at
4.78
BB080923


1428447_at
4.93
AK017734
Tmem14a
1435650_at
4.78
BB082407
Hapln4

1417219_s_at
4.93
NM_025284
Tmsb10
1418184_at
4.78
NM_025639
2610019I03Rik

1418281_at
4.93
NM_011234
Rad51
1436671_at
4.77
BB151200
A030011M19

1423035_s_at
4.93
AK004219
Txnl5
1420124_s_at
4.77
C85065
Tcta

1434362_at
4.92
BM249454
AW550831
1455825_s_at
4.77
BB131619
Lnx1

1435429_x_at
4.92
AV111399
Rps271
1428699_at
4.77
AW744143
Sms

1451931_x_at
4.92
M69068
H2-D1
1419124_at
4.77
NM_133829
2210010L05Rik

1457230_at
4.92
BB458901
Gnpda2
1437360_at
4.76
BB053591
Pcdh19

1436105_at
4.91
BG068839
2310015A05Rik
1451643_a_at
4.76
BC007147
Rab4b

1420873_at
4.91
BI662615
Ptk9
1434826_at
4.76
AW124912
AI256775

1451609_at
4.91
BC024685
Tspan33
1429520_a_at
4.75
BE573407
Phca

1424334_at
4.90
BC010346
Tspan17
1436341_at
4.75
BM125569
F830020C16Rik

1423082_at
4.90
AW545361
Derl1
1426187_a_at
4.75
AF465243
Hax1

1418795_at
4.90
BB487604
Cds2
1416849_at
4.75
NM_134007
D10Ertd214e

1427894_at
4.90
AK012169
Slitl2
1440815_x_at
4.74
BB099075
LOC545039

1451353_at
4.89
AV378394
Tm6sf1
1416503_at
4.74
NM_016753
Lxn

1438928_x_at
4.88
BB252065
Ninj1
1417593_at
4.74
NM_019742
Tusc2

1448887_x_at
4.88
NM_019502
Fxc1
1439963_x_at
4.73
BB660640
1700129I04Rik

1453317_a_at
4.87
AK014353
Khdrbs3
1458396_at
4.73
BB452660
Ssrp1

1435755_at
4.87
BB609468
1110001A16Rik
1429916_at
4.73
AK021129
9530077C05Rik

1418927_a_at
4.87
NM_019986
Habp4
1450868_at
4.73
BF468249
D8Ertd354e

1425118_at
4.87
BC026502
Spire2
1423170_at
4.73
AV213552
Taf7

1428605_at
4.86
AI227096
1810023B24Rik
1427230_at
4.73
BB322737
B930041F14Rik

1429984_at
4.86
AK017666
5730455013Rik
1448619_at
4.73
NM_007856
Dhcr7

1435750_at
4.85
BB610454
Gchfr
1417541_at
4.73
NM_008234
Hells

1417959_at
4.85
NM_026131
Pdlim7
1430574_at
4.72
AK010426
Cdkn3

1415681_at
4.85
NM_053164
Mrpl43
1415947_at
4.72
BC027426
Creg1

1429600_at
4.85
AK003824
1110019K23Rik
1426541_a_at
4.71
BF168366
2310067E08Rik

1442379_at
4.85
BB020678
MGC99845
1416965_at
4.71
AF181560
Pcsk1n

1434342_at
4.84
BB316114

1418743_a_at
4.71
NM_021344
MGI:1930803

1452272_a_at
4.84
BI901126
Gfer
1419056_at
4.71
AF038538
Rtn2

1426416_a_at
4.84
AV216410
Yipf4
1460639_a_at
4.71
NM_009720
Atox1

1431611_a_at
4.84
AK013775
Igsf4a
1424496_at
4.71
BC026742
5133401N09Rik

1438210_at
4.84
BB126999
Gpr149
1460711_at
4.70
BG071611
4930461P20Rik

1427921_s_at
4.83
AW045976
2310061C15Rik
1452976_a_at
4.70
AK004710
Slc9a3r2

1444908_at
4.83
BM228113
Habp4
1433950_at
4.70
BB769866
Igsf21

1417103_at
4.83
NM_010027
Ddt
1423759_a_at
4.70
BC020098
Tmco1

1428157_at
4.83
AV021455
Gng2
1430274_a_at
4.70
AK018331
Stard3nl

1415910_s_at
4.82
NM_134141
Ciapin1
1416696_at
4.70
NM_080837
D17Wsu104e

1416379_at
4.81
NM_019482
Panx1
1427943_at
4.70
BI730288
Acyp2

1424441_at
4.81
BC023114
Slc27a4
1424197_s_at
4.69
BC016538
Fance

1449536_at
4.80
NM_032397
Kcnn1
1448950_at
4.69
NM_008362
Il1r1

1457052_at
4.80
BG064867
AW536275
1447541_s_at
4.69
AV210813
Itgae

1449448_at
4.80
BC019367
Med9
1433635_at
4.68
BG073188
Wdr18

1429213_at
4.80
AK009547
2310030N02Rik
1448700_at
4.68
NM_008059
G0s2

1438713_at
4.79
BB391868
Rassf8





1450397_at
4.79
BB731480
Mtap1b












































































































































































































































































































































































































































































































































































TABLE 20



Top 1,000 cerebellar Purkinje (Pcp2) cell translated

mRNAs enriched when compared to a reference sample

ProbeSymbol = Affymetrix probe identification corresponding to gene symbol; FEN = fold enrichment when

compared to reference sample; Genbank = Genbank identification; GeneSymbol = official gene symbol.










ProbeSymbol
FEN
Genbank
GeneSymbol
ProbeSymbol
FEN
Genbank
GeneSymbol












1438405_at
520.4
BB791906
Fgf7
1446771_at
44.34
BB047533


1450079_at
441.1
AK012873
Nrk
1457011_at
44.01
BB797251


1440436_at
353.6
BQ176471
A730030A06
1424443_at
43.15
AV378394
Tm6sf1

1449240_at
333.9
BC026822
MGI:1333876
1417552_at
42.43
NM_007986
Fap

1427624_s_at
304.2
AJ249492
Il22 ; Iltifb
1447405_at
42.03
BB479096


1420994_at
301
BM214359
B3gnt5
1442751_at
41.29
AI587721
D430034N21

1432515_at
298.4
AK010774
2410124H12Rik
1449102_at
40.59
U71189
Ebf2

1419084_a_at
289.1
NM_008790
Pcp2
1437836_x_at
38.92
BB357560
0610011L14Rik

1436410_at
254.9
BB346846
AI449023
1453127_at
38.89
AK009235
Ppm1j

1418095_at
223.9
NM_025357
Smpx
1433579_at
38.28
BG068678
Tmem30b

1427291_at
180.5
AV275615
Sycp1
1437254_at
38.26
BB315525
6230425F05Rik

1435750_at
168.7
BB610454
Gchfr
1416892_s_at
37.97
BC021353
3110001A13Rik

1424958_at
168.2
BC010773
Car8
1459589_at
37.6
C85932
Cfyl1

1424859_at
155.8
BC005773
Homer3
1438708_x_at
37.14
BI465579
Ywhab

1457904_at
144.2
BB125515

1423452_at
37.03
AV173139
Stk17b

1455970_at
134.4
BE370618

1445913_at
36.44
AV251751
A730090H04Rik

1458341_x_at
130.3
BB397841

1433553_at
36.26
BB131106
Garnl3

1443699_at
119.9
BE957383
Ptprk
1427760_s_at
35.5
X75557
Plf ; Plf2 ;

1419518_at
109.9
NM_017379
Tuba8



Mrpplf3

1458284_at
108.9
BM195499
Ptbp1 ;
1419061_at
35.41
D89821
Rhod




LOC236294
1455431_at
33.78
AV371434
Slc5a1

1450428_at
106.6
AV335209
Lhx1
1458434_at
33.56
BB245484
A730009L09Rik

1457523_at
98.38
BB125017
9530056K15Rik
1456798_at
32.78
BB317517
9330118A15Rik

1448509_at
96.98
BC021353
3110001A13Rik
1455758_at
32.74
BM215011


1439232_at
93.96
BB781460

1425469_a_at
32.47
BC003855


1437209_at
89.42
AV324566
6230425F05Rik
1454884_at
32.03
BG073185
Btbd4

1435275_at
84.82
AV013496
Cox6b2
1430310_at
32.02
BB535262
Tspan11

1438340_at
80.48
BB277600
A930006D11
1457337_at
31.88
AV028487


1447742_at
79.1
BB216290
Laptm5
1453223_s_at
31.6
AK010743
Dppa2

1429346_a_at
78.36
BB733638
4933440J22Rik
1421951_at
31.31
AV335209
Lhx1

1419057_at
77.86
AF208031
Slc5a1
1431197_at
31.19
BB641782
Arl6ip2

1450078_at
77.37
AK012873
Nrk
1443824_s_at
31.1
BB193643
Car7

1425179_at
75.87
AF237702
Shmt1
1445576_at
30.75
AI413817
4930526H21Rik

1455512_at
75.01
AI852151

1417597_at
30.24
NM_007642
Cd28

1418173_at
69.35
NM_133730
4631426H08Rik
1429719_at
30.19
AK009204
Foxp4

1441776_at
68.9
BB732603
Tspan11
1440727_at
30.1
BF472124


1434973_at
68.36
BE650380
Car7
1424046_at
29.81
AF002823
Bub1

1440944_at
66.97
BF463236
LOC544718
1447439_at
29.67
AI848394
1700023E05Rik

1450997_at
66.54
AV173139
Stk17b
1419326_at
29.63
NM_026188
1700028P14Rik

1460118_at
63.38
BF455409

1427676_a_at
29.62
X66118
Grik1

1418494_at
61.18
U71189
Ebf2
1420348_at
29.14
NM_008499
Lhx5

1436035_at
57.75
AV225683
3830431G21Rik
1438773_at
28.87
BB817972
Steap2

1427707_a_at
57.14
BC004585
Sil
1416893_at
28.74
BC021353
3110001A13Rik

1434588_x_at
52.98
AI181686
Tbca
1439745_at
28.58
AV338037
Cacng7

1452484_at
52.2
AF291660
Car7
1448738_at
28.2
BB246032
Calb1

1459995_at
51.58
AV047656
1700015G11Rik
1460482_at
27.39
AW123948
3110047P20Rik

1457558_at
51.52
AV325555
A330050F15Rik
1437025_at
27.27
AV313615
Cd28

1437580_s_at
51.44
C77054
Nek2
1455607_at
26.98
BG072958
Rspo3

1423366_at
50.63
BE133651
Scd3
1417299_at
26.87
NM_010892
Nek2

1422243_at
49.69
NM_008008
Fgf7
1458667_at
26.77
AV266695
4930519N13Rik

1417843_s_at
47.32
NM_133191
Eps812
1443999_at
26.77
BB203873
Nek2

1441166_at
46.97
AW123460
A330050F15Rik
1421129_a_at
26.42
NM_016745
Atp2a3

1433639_at
46.62
AW548096
5730593F17Rik
1456834_at
26.03
BB277828
Ibrdc2

1422481_at
46.07
NM_008473
Krt2-1
1452404_at
25.64
BB321846
Phactr2

1418933_at
45.46
NM_009200
Slc1a6
1453904_at
25.52
AK015926
4930528G09Rik

1428536_at
44.48
AK015001
Kcng4
1441785_at
25.37
AW120650


1423978_at
25.35
BC025837
Sbk1
1422752_at
18.77
BG070121
Polr3k

1441236_at
25.32
AV373598
Slc9a3
1433582_at
18.76
AV309085
1190002N15Rik

1452986_at
25.31
AK002584
Hgd
1455952_at
18.69
AI503324
Adprhl1

1446612_at
25.06
BB169371
9330118A15Rik
1449874_at
18.66
NM_016923
Ly96

1426015_s_at
24.98
AF302653
Asph
1435959_at
18.4
BM246535
Arhgap15

1416794_at
24.9
NM_019717
Arl6ip2
1436326_at
18.27
BB306272
Rora

1428349_s_at
24.75
AK014058
Ebf3
1437880_at
18.02
AV273001
Lbxcor1

1429262_at
24.27
AK005472
Rassf6
1433892_at
18.02
BM208112
Spag5

1438558_x_at
24.07
AV009267
Foxq1
1418237_s_at
17.9
NM_009929
Col18a1

1426047_a_at
23.71
AF129509
Ptprr
1421392_a_at
17.78
NM_007464
Birc3

1440815_x_at
23.69
BB099075
LOC545039
1452889_at
17.75
AK009207
2310007H09Rik

1446894_at
23.56
BB117494
Arnt2
1437444_at
17.74
BB479976
Pcdhgc3

1442209_at
23.42
BB251707
2400009B08Rik
1448355_at
17.71
NM_019429
Prss16

1456729_x_at
23.29
BB218661
Rtel1
1426955_at
17.58
D17546
Col18a1

1418957_at
23.25
NM_016853
Stac
1420334_at
17.54
NM_134251
Slc12a8

1429712_at
23.19
AK005093
Etohi1
1418733_at
17.53
NM_011658
Twist1

1421309_at
22.81
NM_008598
Mgmt
1451931_x_at
17.43
M69068
H2-D1

1447828_x at
22.69
BB226589
2310005P05Rik
1419985_s_at
17.41
C87579
D11Ertd461e

1439181_at
22.67
AV020760
BC043301
1436716_at
17.3
BM941586
Ppp1r14b

1443939_at
22.58
BF021397
LOC230628
1445173_at
17.28
BF472675
AK129341

1443591_at
22.42
BB827848
Dlx4
1452704_at
17.28
AK004786
1200015F23Rik

1420052_x_at
22.4
C81484
Psmb1
1422529_s_at
17.26
NM_009814
Casq2

1433605_at
22.29
BB307136
Inpp5a
1455977_x_at
17.2
AV053098
Klk6 ; Klk5

1450956_at
21.88
BE133651
Scd3
1437101_at
17.18
AV271979
Lats2

1436627_at
21.84
BB368203
D17Ertd663e
1439146_s_at
16.94
AA867167
Lck

1440688_at
21.83
BM244666
Arhgap26
1439729_at
16.77
BB463084
A930038B10Rik

1439834_at
21.73
AA261399
2400009B08Rik
1439377_x_at
16.68
BB041150
Cdc20

1454171_x_at
21.52
AK020608
9530053H05Rik
1448459_at
16.6
NM_027398
Kcnip1

1433588_at
21.11
BM238232
D6Wsu116e
1422537_a_at
16.52
NM_010496
Id2

1460666_a_at
20.9
NM_010096
Ebf3
1429166_s_at
16.42
AW494443
Clmn

1437250_at
20.87
AV298358
MGI:2151839
″AFFX-




1416698_a_at
20.85
NM_016904
Cks1b
DapX-3_at″
16.41
AFFX-DapX-


1447178_at
20.83
BB173057



3


1459801_at
20.67
BB189298
B3galt5
1435487_at
16.38
BB049642
Grid2

1448117_at
20.66
BB815530
Kitl
1417088_at
16.37
AW493043
Zfp346

″AFFX-r2-



1428977_at
16.23
AK005217
Chst8

Bs-dap_3 at″
20.63
AFFX-r2-Bs-

1439266_a_at
16.18
AV260647
Polr3k



dap-3

1423790_at
16.14
BC024876
Dap

1451683_x at
20.56
M34962
H2-D1
1455101_at
16.12
BE631955


1424904_at
20.35
BC019143
1300010F03Rik
1459660_at
16.05
BE944772


1417178_at
20.32
NM_016867
Gipc2
1457441_at
16.04
AI385540
Ebf1

1420993_at
20.27
BM214359
B3gnt5
1431446_at
16
AK002366
0610009B14Rik

1446071_at
20.07
BB529332
Steap2
1428196_a_at
15.96
AK004786
1200015F23Rik

1454653_at
19.99
BB274531
A730016F12Rik
1415947_at
15.95
BC027426
Creg1

1454079_at
19.88
AK016591
4933400F03Rik
1450389_s_at
15.78
NM_008846
Pip5k1a

1451112_s_at
19.86
BC024876
Dap
1420453_at
15.73
NM_009967
Crygs

1419473_a_at
19.72
NM_031161
Cck
1418709_at
15.68
AF037370
Cox7a1

1425198_at
19.7
BG076152
Ptpn2
1424826_s_at
15.5
BC024131
Mtss1

1441836_x_at
19.66
AV045004
1700006H03Rik
1454713_s_at
15.43
BG072171
Hdc

1447004_at
19.26
BB128185

1420583_a_at
15.35
NM_013646
Rora

1433893_s_at
19.24
BM208112
Spag5
1451707_s_at
15.31
BC011108
Slc41a3

1415855_at
18.97
BB815530
Kitl
1434201_at
15.21
AV144145
Chrdl1

1426738_at
18.96
BC014860
Dgkz
1417164_at
15.19
NM_022019
Dusp10

1420664_s_at
18.94
NM_011171
Procr
1421446_at
15.12
NM_011102
Prkcc

1417577_at
18.9
NM_019510
Trpc3
1444790_at
15.08
AV048499
1810005K13Rik

1443560_at
18.77
BB283805
Pofut2
1420522_at
15.06
NM_026202
2610529H08Rik

1450276_a_at
15.03
NM_009132
Scin
1434006_at
12.27
BQ030992
BC051227

1427178_at
14.89
BB667469
Tmc4
1436948_a_at
12.14
BB520013
6430550H21Rik

1444582_at
14.68
AV032530
4833420K19Rik
1439254_at
12
BE981392
Akap13

1435226_at
14.46
BG064140
Ibrdc3
1452751_at
11.99
AK014058
Ebf3

1423365_at
14.4
AW494038
Cacna1g
1424037_at
11.97
BC027291
Itpka

1423652_at
14.39
AV209097
Hb1d2
1451776_s_at
11.96
BC024546
MGI:1916782

1443798_at
14.38
BB207248
Pik3cd
1421834_at
11.96
NM_008846
Pip5k1a

1434950_a_at
14.35
BE995635
Armc8
1428268_at
11.86
AK018116
Psd2

1434034_at
14.27
BI905090
Cerk
1433589_at
11.85
BM238232
D6Wsu116e

1417279_at
14.26
NM_010585
Itpr1
1422409_at
11.84
NM_008237
Hes3

1450896_at
14.19
BM248774
Arhgap5
1423062_at
11.76
AV175389
Igfbp3

1425545_x_at
14.14
M86502
H2-D1 ; H2-L
1438035_at
11.73
BB748934
AW061290

1419017_at
14.14
NM_016869
Corin
1456631_at
11.65
AV272901
LOC381279

1449577_x_at
14.02
AK003186
Tpm2
1425440_x_at
11.61
BC011108
Slc41a3

1426923_at
14.02
BB130716
Hrb
1446441_at
11.55
BB126659
Slc20a1

1426500_at
13.95
BF462080
Icmt
1458918_at
11.52
BB552741
Slc12a8

1415948_at
13.89
BC027426
Creg1
1451602_at
11.5
BC025911
Snx6

1458129_at
13.86
BB559501
Rora
1452462_a_at
11.45
BC013339
Banp

1416302_at
13.81
BB125261
Ebf1
1418254_at
11.43
NM_019735
Apip

1420595_at
13.79
NM_007867
Dlx4
1452478_at
11.42
AY044451
Alpk2

1421996_at
13.77
NM_011547
Tcfap2a
1428064_at
11.41
BI248650
Centd2

1437987_at
13.73
BB389497

1455455_at
11.39
BB008997
Glt28d2

1441786_at
13.7
AW048005

1450914_at
11.37
BE986849
Ppp1r14b

1452890_at
13.66
AK009255
Ttll5
1455343_at
11.37
BF159528
Plekha7

1436690_at
13.55
BB494139
Lrba
1447282_at
11.36
BE979542
Chtf18

1418758_a_at
13.43
NM_011182
Pscd3
1416785_at
11.34
NM_027398
Kcnip1

1419999_at
13.4
C81413
Igbp1
1448799_s_at
11.24
NM_011885
Mrps12

1439959_at
13.31
AW488620
Fgf11
1457232_at
11.22
BE946365
Fbxl21

1438036_x_at
13.29
BB748934
AW061290
1438812_x_at
11.2
BB771589
Usp19

1447625_at
13.25
BB286270
E2f5
1434273_at
11.18
BG073439
A830073O21Rik

1439447_x_at
13.19
AV056887
Rpl37a
1438987_at
11.17
AV044525
4921509E07Rik

1421436_at
13.13
NM_008167
Grid2
1416645_a_at
11.16
NM_007423
Afp

1456934_at
13.11
BB177770

1426324_at
11.01
M33151
H2-D1

1416160_at
13.1
AI463873
Nr2f2
1416664_at
10.97
NM_023223
Cdc20

1418831_at
13.06
AW475993
Pkp3
1428345_at
10.96
BB229589
4932443D16Rik

1436867_at
13
BG795043
Srl
1450852_s_at
10.9
BQ173958
F2r

1432478_a_at
12.96
AK015966
Ibrdc3
1443187_at
10.89
AV235077
Rspo3

1416301_a_at
12.96
BB125261
Ebf1
1420653_at
10.89
NM_011577
Tgfb1

1452362_at
12.89
BB834440
Trim16
1453829_at
10.89
BE948719
2310007J06Rik

1442502_at
12.89
BB011593
Lrch1
1434036_at
10.72
AV024771
Mtss1

1426981_at
12.86
BI157485
Pcsk6
1450310_at
10.69
NM_133355
Grid2ip

1421435_at
12.86
NM_008167
Grid2
1456596_at
10.63
BB093996
6430550H21Rik

1439789_at
12.85
BQ177189

1453409_at
10.62
AK004156
Cgrrf1

1423536_at
12.67
BF148627
Strn3
1433581_at
10.62
AV309085
1190002N15Rik

1438073_at
12.66
AW047633

1457118_at
10.61
AV353605
6230417E10Rik

1439699_at
12.66
BB525237
Pgr
1421078_at
10.56
NM_053085
Tcf23

1451784_x_at
12.65
L36068
H2-D1 ; H2-K1 ;
1438151_x_at
10.55
BB544336
Zdhhc14




H2-L ;
1424709_at
10.52
AB016248
Sc5d




LOC56628
1436399_s_at
10.52
BI901194
Nrk

1428636_at
12.62
AK015015
Steap2
1442015_at
10.49
BG070940


1460084_at
12.62
BB473929

1455165_at
10.45
BE335227
Rora

1433764_at
12.6
BB026221
Clec21
1457942_at
10.42
BI467159
Pdcl

1453936_at
12.6
BQ032796

1452095_a_at
10.37
AK005204
H47

1455775_at
12.55
AW988196

1439849_at
10.33
BB215381


1431241_at
12.29
AA692147
Chchd3
1433919_at
10.31
AV302111
Asb4

1422426_at
12.29
NM_020612
Cmar
1455637_x_at
10.29
BB465277


1442823_at
10.24
BB536648

1420667_at
8.751
BM117900
Doc2b

1451426_at
10.23
AF316999
D11Lgp2e
1418233_a_at
8.719
AV226526
Trappc5

1431167_at
10.16
AI646503
Dgkg
1451163_at
8.717
AF214013
Tinf2

1440827_x_at
10.15
BB018032
Sox5
1449461_at
8.67
NM_023462
Rbp7

1449872_at
10.14
NM_019960
Hspb3
1445218_at
8.661
BE955408
Vax2

1415887_at
10.12
NM_019678
Tfg
1436281_at
8.642
BB465277


1441622_at
10.05
AV374024
LOC433023
1437841_x_at
8.624
BB248249
Csdc2

″AFFX-r2-



1438502_x at
8.618
AA030209
Rps17 ;

Bs-phe-3_at″
10.02
AFFX-r2-Bs-




LOC383032



phe-3

1443197_at
8.608
BB501165
Wnt9b

1416158_at
10.02
AI463873
Nr2f2
1431073_at
8.594
AK015225
4930428J16Rik

1458753_at
9.948
BB548169
Gm941
1425028_a_at
8.571
BC024358
Tpm2

1422753_a_at
9.868
BG070121
Polr3k
1436112_at
8.555
BB128317
AI118078

1441025_at
9.865
BB333919
3110047P20Rik
1425891_a_at
8.555
AF329833
Grtp1

1422157_a_at
9.816
NM_008403
Itgb1bp1
1417147_at
8.535
NM_019833
B230317C12Rik

1432355_at
9.808
AK008780
2210039B01Rik
1452244_at
8.517
AK018128
6330406115Rik

1453060_at
9.804
AK018337
Rgs8
1439441_x_at
8.505
BB134767
Lats2

1423422_at
9.794
AV113827
Asb4
1417430_at
8.503
NM_007672
Cdr2

1424541_at
9.785
BC011320
Tmem70
1425779_a_at
8.469
AF326960
Tbx1

1450897_at
9.748
BM248774
Arhgap5
1444730_at
8.421
BB297867
Tlk1

1416131_s_at
9.717
BB188557
C920006C10Rik
1435517_x_at
8.385
BB465250
Ralb

1458395_at
9.717
BB811756
B930054O08
1434881_s_at
8.365
BM220945
Kctd12

1458595_at
9.712
AW907704
Pdzk6
1436895_at
8.353
BB182934
Centd1

1449876_at
9.684
NM_011160
Prkg1
1437392_at
8.352
AW123113
LOC433485

1443733_x_at
9.655
C85233
Pold3
1416398_at
8.346
BB474887
Mesdc1

1458677_at
9.609
BB517186
Entpd5
1426374_at
8.34
AK010825
2410166I05Rik

1428601_at
9.415
AK005628
1700003E16Rik
1444112_at
8.331
BQ174086


1449202_at
9.38
NM_011976
Sema4g
1459107_at
8.323
BG069378
Kcnh3

1434110_x_at
9.284
BF322785
Mup1
1448160_at
8.305
NM_008879
Lcp1

1430780_a_at
9.284
B1739353
Pmm1
1416121_at
8.303
M65143
Lox

1434014_at
9.27
BB291836
Atg4c
1416793_at
8.293
NM_019717
Arl6ip2

1427594_at
9.263
BC002224
Zfp697
″AFFX-r2-




1455164_at
9.239
AV308092
Cdgap
Bs-dap-M_at″
8.268
AFFX-r2-Bs-


1429884_at
9.16
AK005172
Srgap2


dap-M


1441161_at
9.152
BB307791
B230216G23Rik
1424350_s_at
8.259
BE987427
Lpgat1

1442042_at
9.113
BG092211

1436728_s_at
8.218
BG071028
Rtel1

1451536_at
9.105
AK009195
Mtfr1
1447864_s_at
8.212
AV377712
Pogk

1419660_at
9.097
NM_025904
1600012F09Rik
″AFFX-




1425017_at
9.095
BB314809
Pcdhac1
DapX-M_at″
8.187
AFFX-DapX-


1451147_x_at
9.037
BC016109
Csdc2


M


1435735_x_at
9.034
BB285733
H47
1460735_at
8.169
BM203457
Svil

1449307_at
9.014
NM_028146
2810427I04Rik
1423194_at
8.139
BM248774
Arhgap5

1429156_at
8.998
BF453953
2610036L11Rik
1452642_at
8.115
BE372017
Tmem16f

1430125_s_at
8.981
AK009256
Pqlc1
1460647_a_at
8.106
NM_010150
Nr2f6

1449403_at
8.978
NM_008804
Pde9a
1419647_a_at
8.096
NM_133662
Ier3

1450702_at
8.907
AJ306425
Hfe
1427940_s_at
8.082
BB046347
Mycbp

1448704_s_at
8.889
NM_024439
H47
1448812_at
8.055
NM_016677
Hpcal1

1440725_at
8.882
BB131092
Gpr63
1419614_at
8.039
BC021592
Pla2g12b

1450837_at
8.853
NM_031499
Prh1
1434064_at
8.026
BQ175677
BC061259

1439006_x_at
8.849
BB093996
6430550H21Rik
1437683_x_at
8.004
AV047585
Serf2

1444363_at
8.848
BB269445

1453528_at
7.985
AK005161
Lta4h

1428760_at
8.831
AW537061
Snapc3
1435208_at
7.966
AV327407
Dtx3l

1420058_s_at
8.83
AI480750
2410166I05Rik
1423535_at
7.959
BF148627
Strn3

1459300_at
8.784
BB226459

1420566_at
7.945
NM_025367


1447296_at
8.755
AW490725
Fbxo27
1435671_at
7.945
BB526605
Mipol1

1459592_a_at
8.755
BB752796
LOC547217
1456731_x_at
7.932
AV169424
Polr3k

1437914_at
7.923
BB104097
E2f6
1446786_at
7.197
BG073077
Aplp2

1450500_at
7.916
AW012751
Uhmk1
1449347_a_at
7.19
NM_021365
Xlr4b ; Xlr4a

1420666_at
7.908
BM117900
Doc2b
1455056_at
7.188
BM231903
Lmo7

1443766_x_at
7.895
BB311234
Rab11fip4
1419406_a_at
7.177
NM_016707
Bcl11a

1454842_a_at
7.884
AI853240
B3galnt2
1451353_at
7.171
AV378394
Tm6sf1

1426048_s_at
7.883
BC021623
Tcfap2a
1452451_at
7.168
AF240460
MGI:1929597

1445728_at
7.88
BB667663

1435491_at
7.167
BI076615
AI875089

1416159_at
7.878
AI463873
Nr2f2
1438501_at
7.166
AA030209


1430489_at
7.83
BB086802
5430416G10Rik
1455710_x_at
7.16
AI528732
Mtcp1

1416542_at
7.826
NM_009343
Phf1
1449079_s_at
7.154
NM_018784
St3gal6

1425197_at
7.813
BG076152
Ptpn2
1417653_at
7.141
NM_013645
Pvalb

1440735_at
7.812
BB283168
Polr3k
1419836_at
7.105
AU040583


1425177_at
7.809
AF237702
Shmt1
1435594_at
7.1
AV334690
Arl6ip2

1454161_s_at
7.733
AK002308
0610007P14Rik
1456251_x_at
7.095
BB132602
Bzrp

1439425_x_at
7.729
AV100801
BC024814
1451190_a_at
7.063
BC025837
Sbk1

1418909_at
7.722
NM_013848

1417344_at
7.009
BE915256
2900064A13Rik

1450235_at
7.718
NM_015759
Fgd3
1455850_at
7.006
BG073353
2310003H01Rik

1420304_x_at
7.709
AV128236

1437308_s_at
7
AV024285
F2r

1456722_at
7.685
BI966456
Chrdl1
1458358_at
6.994
BB402666
Pank2

1452827_at
7.657
AK005178
1500009C09Rik
1417121_at
6.991
NM_008068
Gabra6

1449176_a_at
7.637
NM_007832
Dck
1447753_at
6.987
BB391093
Cdc37l1

1457341_at
7.636
BE991676

1431172_at
6.987
BB620704
Orc4l

1417960_at
7.623
NM_007755
Cpeb1
1431299_a_at
6.979
AK009340
2310014H01Rik

1449584_at
7.562
NM_138650
Dgkg
1420059_at
6.964
AI480750
2410166I05Rik

1438698_at
7.547
AV305379
4632425D07Rik
1418932_at
6.936
AY061760
Nfil3

1433623_at
7.546
BE629588
Zfp367
1443714_at
6.935
AI451920


1424902_at
7.481
AF378760
Plxdc1
1423055_at
6.917
NM_010942
Nsg1

1451796_s_at
7.479
AF109137
Hdc
1429204_at
6.905
AK013788
2900075A18Rik

1443846_x_at
7.477
BB402666
Pank2
1426032_at
6.897
AF289078
Nfatc2

1421833_at
7.473
NM_008846
Pip5k1a
1456337_at
6.896
AV291818
Centd1

1458536_at
7.455
BB097972
Ccni
1440700_a_at
6.895
BB227996
Arhgef18

1422941_at
7.449
NM_053116
Wnt16
1419880_x_at
6.893
AA204020


1455448_at
7.415
BG804066
MGI:2677061
1449621_s_at
6.892
AW121720
Thsd1

1436322_a_at
7.391
BE952576
2810001A02Rik
1426189_at
6.89
AF357887
Dusp15

1426937_at
7.383
AK018128
6330406I15Rik
1455160_at
6.882
BM220421
2610203C20Rik

1441271_at
7.371
C78231
Idh3b
1460462_at
6.871
AK012903
Med18

1458328_x_at
7.36
BE993003
3110007P09Rik
1427556_at
6.869
BC019408
Mylk2

1424692_at
7.351
BC004753
2810055F11Rik
1419742_at
6.862
NM_026091
1700037H04Rik

1416632_at
7.335
BC011081
Mod1
1437935_at
6.85
BB821151
4930486G11Rik

1425737_at
7.324
BC002195
2510016G02Rik
1454692_x_at
6.844
BB722680


1454866_s_at
7.313
BQ176424
Clic6
1437942_x_at
6.843
AV100095
Tube1

1417876_at
7.307
AF143181
Fcgr1
1424216_a_at
6.831
U52197
Papola

1419422_at
7.301
BB706784
Pkd2l2
1436829_at
6.82
BB667112
Trim67

1428948_at
7.297
BE952741
5730414M22Rik
1428452_at
6.813
AK012812
2810025M15Rik

1438967_x_at
7.297
AV259665
Amhr2
1432059_x_at
6.775
AK017143
5031425E22Rik

1442614_at
7.297
BE945253
Il1rap
1453554_a_at
6.771
AK009297
Wdr33

1424034_at
7.297
BI660199
Rora
1452016_at
6.759
BC026209
Alox5ap

1430827_a_at
7.271
AV301702
Ptk2
1434877_at
6.746
AI152800
Nptx1

1428197_at
7.263
AK020159
Tspan9
1449836_x_at
6.743
NM_007546
Biklk

1431226_a_at
7.26
AK013203
Fndc4
1428604_at
6.739
AK011986
2610305D13Rik

1459875_x_at
7.25
AV333371

1418028_at
6.735
NM_010024
Dct

1432271_a_at
7.247
AK012764
Dcun1d5
1425178_s_at
6.732
AF237702
Shmt1

1436528_at
7.222
AI842353
Kazald1
1438948_x_at
6.73
AV101079
Bzrp

1426922_s_at
7.212
BB130716
Hrb
1442376_at
6.717
BB560441
Ablim1

1430490_at
7.21
AK017409

1424784_at
6.709
AV047635
1700029I01Rik ;

1423845_at
7.202
BC016109
Csdc2



LOC433791 ;




LOC545705
1419756_at
6.348
NM_138650
Dgkg

1427878_at
6.695
AK002512
0610010O12Rik
1416848_at
6.343
NM_025401
Ubl5

1457330_at
6.682
BB014986
Semcap2
1420774_a_at
6.34
NM_026358
4930583H14Rik

1436141_at
6.681
BM934616
2610510L01Rik
1417222_a_at
6.34
NM_133739
2310075C12Rik

1442099_at
6.681
BM227490
Usp31
1430866_at
6.339
BB432681
4921537D05Rik

1437642_at
6.674
BB243375
Hrbl
1436087_at
6.328
BQ176414
Dpp10

1450168_at
6.666
NM_025971
Ankrd12
1439117_at
6.328
AU067755
Clmn

1436536_at
6.665
AW108488
C730015A04Rik
1415854_at
6.321
BB815530
Kitl

1425701_a_at
6.662
AF350047
Rgs3
1431229_at
6.286
AK019361
C030032C09Rik

1442791_x_at
6.657
BF459282
6720407P12Rik
1431356_at
6.282
AK019889
6430710C18Rik

1454939_at
6.643
BB268102
Phf20l1
1460262_a_at
6.274
NM_026091
1700037H04Rik

1421360_at
6.636
NM_030266
Inpp4a
1450115_at
6.267
NM_008139
Gnaq

AFFX-ThrX-
6.611
AFFX-ThrX-3

1418247_s_at
6.246
BG277926
Rbm9

3_at



1439837_at
6.242
BE136147
Tnrc15

1450537_at
6.601
AF196480
Mid2
1430519_a_at
6.228
AK007767
Cnot7

1457894_at
6.601
BB284616

1422313_a_at
6.222
NM_010518
Igfbp5

1433792_at
6.597
AW491344
Nrip2
1439825_at
6.209
BB705351
Dtx3l

1416846_a_at
6.59
NM_018884
Pdzn3
1448613_at
6.205
NM_007899
Ecm1

1459842_x_at
6.588
AV035368
Nubp2
1460479_at
6.201
AK020726
A330094K24Rik

1433429_at
6.584
BB309245
Pigs
1423020_at
6.188
NM_053120


1431279_s_at
6.569
AK016145
Ttll5
1424604_s_at
6.18
BC026981
Sumf1

1420020_at
6.559
AU022339
Suz12
1453301_a_at
6.178
AK008083
2010004B12Rik

1431421_x_at
6.55
BI082843
2610524G07Rik
1450622_at
6.176
NM_009954
Bcar1

1454219_at
6.543
AK013295
Dnajc2
1418760_at
6.168
AB030503
Rdh11

1431088_at
6.536
BQ286886
Foxp4
1439479_at
6.16
C78577
Lct

1451840_at
6.535
BG261945
Kcnip4
1424846_at
6.159
BC027174
BC027174

1431293_a_at
6.519
AK012260
1110019C08Rik
1458268_s_at
6.133
AI649005
Igfbp3

1439078_at
6.513
BB037068
Klhl4
1418007_at
6.125
BM932567
1810007M14Rik

1435621_at
6.513
BQ175346
Mlstd1
1435092_at
6.125
AV328143
Arl4

1457290_at
6.49
BB127590
Mab21l1
1448487_at
6.124
NM_008515
Lrrfip1

1436347_a_at
6.485
BB501229
5530601H04Rik
1415791_at
6.122
NM_030564
Rnf34

1458320_at
6.47
BB012140

1427593_at
6.114
BB620112
Trim8

1454638_a_at
6.464
AW106920
Pah
1437762_at
6.106
BB130995
Rab39

1457256_x_at
6.463
BB530125
Ptch2
1453024_at
6.105
AK014128
Wdr37

1429583_at
6.46
BE865033
LOC554362
1428344_at
6.093
BB229589
4932443D16Rik

1433836_a_at
6.447
AV365503
8430408G22Rik
1448441_at
6.086
NM_016904
Cks1b

1427174_at
6.433
BM235840
3100002L24Rik
1423854_a_at
6.085
BC008101
Rasl11b




; LOC329575 ;
1449310_at
6.079
BC005440
Ptger2




LOC433520 ;
1449896_at
6.07
NM_053015
Mlph




LOC433523 ;
1435947_at
6.069
BQ175669





LOC433531 ;
1425439_a_at
6.06
BC011108
Slc41a3




LOC545489 ;
1460382_at
6.06
BC020535
BC020535




LOC545490 ;
1437631_at
6.038
BB188374
Kcnip4




LOC545491 ;
1418202_a_at
6.031
NM_011717
Wiz




LOC545492
1435238_x_at
6.028
AV004950
2310009A05Rik

1423371_at
6.417
BF577544
Pole4
1444601_at
6.02
AI463033


1419399_at
6.416
AW553649
Mttp
1459468_at
5.999
C79743
C79743

1456926_at
6.4
BB049544
Rims4
1453591_at
5.98
AK017626
5730437N04Rik

1442160_at
6.4
AV360034
7530404M11Rik
1431707_a_at
5.976
AK016645
Pscd3

1451601_a_at
6.399
BC025823
BC011467
1451085_at
5.974
BC019462
C030006K11Rik

1436661_at
6.394
BQ174532
Dpp10
1426544_a_at
5.974
BB118847
Ttc14

1430803_at
6.391
BB074761
2810403D23Rik
1456210_at
5.967
AV222628


1455080_at
6.376
BB375209
Ppp1r16b
1450506_a_at
5.914
NM_026531
Isg20l1

1431642_at
6.373
AV122850
Eif2s3y
1437262_x_at
5.902
AA940256
Bcas2

1452237_at
6.361
BB130716
Hrb
1430763_at
5.9
BB016642
4930563E22Rik

1424191_a_at
6.354
BC019770
Tmem41a
1449259_at
5.896
BB349707
Rab3d

1419305_a_at
5.879
NM_025386
Fbxo36



LOC546355

1424092_at
5.871
BC017137
Epb4.1
1423668_at
5.537
BC021423
Zdhhc14

1417933_at
5.857
NM_008344
Igfbp6
1434165_at
5.523
BQ176424
Clic6

1421281_at
5.851
Z36357
Gabra1
1427170_at
5.521
BF318721
Psma8

1437594_x_at
5.835
BB376188
Pigt
1436160_at
5.516
BB150142
4732407F15Rik

1423229_at
5.835
BM217803
Inpp5e
1423671_at
5.515
AF370126
Dner

1435204_at
5.83
BG065273
Hrmt1l4
1457652_x_at
5.509
BB477203


1449072_a_at
5.828
NM_026526
2510005D08Rik
1438992_x_at
5.507
AV314773
Atf4

1433134_at
5.826
AK017998
5830448L01Rik
1433787_at
5.491
AI841091
B230343H07Rik

1430879_at
5.825
BB283753
C030008P14Rik
1435138_at
5.456
AV016797
Tmem28

1440708_at
5.803
BM121854
Myh9
1418796_at
5.446
NM_009131
Clec11a

1427981_a_at
5.79
AY033912
Csad
1451365_at
5.445
BC025619
Rbm19

1449509_at
5.788
NM_011353
Serf1
1456563_at
5.443
BB769119


1437371_at
5.785
BB008528
9930021J17Rik
1451418_a_at
5.428
BC023083
Spsb4

1451975_at
5.783
BC005638
2810453I06Rik
1421799_at
5.423
NM_011910
Uts2

1439152_at
5.78
BE985292
BC052066
1422093_at
5.421
NM_009522
Wnt3a

1455755_at
5.776
BB026554
Gm88
1444380_at
5.412
AI844869
AI844869

1437595_at
5.762
BB531414
E030010A14Rik
1426552_a_at
5.412
BB772866
Bcl11a

1424229_at
5.748
BC006704
Dyrk3
1457472_at
5.407
BB782031
Tnrc15

1456313_x-at
5.744
BB257397
Mrpl28
1418386_at
5.384
NM_026526
2510005D08Rik

1429971_at
5.731
BE948556
Txnrd2
1435277_x_at
5.377
AV156640
Nme1

1435270_x_at
5.72
BF730076
2510005D08Rik
1436454_x_at
5.372
BB393998
Fen1

1444141_at
5.711
BB828008
Snx13
1421746_a_at
5.366
NM_015796
Fbxo17

1449287_at
5.695
NM_011481
Srms
1432161_a_at
5.363
AK006988
4930428J16Rik

1451363_a_at
5.686
BC008266
Dennd2d
1430156_at
5.351
AK015855
4930520O04Rik

1457433_x_at
5.685
BB187816
Zfp120
1449910_at
5.345
NM_029813
2210418O10Rik

1420150_at
5.685
AI596360
Spsb1
1419739_at
5.342
AK003186
Tpm2

1447771_at
5.679
AW050081

1419913_at
5.339
AW557906
Strap

1418890_a_at
5.678
BB349707
Rab3d
1460652_at
5.338
NM_007953
Esrra

1448986_x_at
5.675
NM_010062
Dnase2a
1438014_at
5.337
BE984852
Mrpl34

1422847_a_at
5.674
AF251036
Prkcd
1418583_at
5.337
NM_025798
Hint3

1439035_at
5.673
BG075830
Zfp322a
1423286_at
5.331
AA016422
Cbln1

1457961_at
5.667
BB713538

1449209_a_at
5.323
AB030503
Rdh11

1449897_a_at
5.664
NM_010839
Mtcp1
1459941_at
5.323
BB096900
4933402J24Rik

1437820_at
5.654
BB040642
Fkhl18
1426901_s_at
5.313
BB657856
Camta2

1440242_at
5.647
BB356428
C030014K22Rik
1419851_at
5.305
W13854
Slc4a8

1428216_s_at
5.645
BB609428
Tomm7
1427997_at
5.305
BG064890
1110007M04Rik

1449362_a_at
5.642
NM_016713
Mink1
1427318_s_at
5.302
BI555209
Dysf ; Fer1l3

1458347_s_at
5.635
BB414224
Tmprss2
1429726_at
5.301
AK004684
Slc16a9

1423384_s_at
5.633
BF181445
Tex261
1426856_at
5.293
BM200015
Hsdl2

1430590_at
5.628
BG228878
D3Ertd751e
1453968_at
5.282
AK019523
4833439F03Rik

1428447_at
5.611
AK017734
Tmem14a
1448999_at
5.281
AV226526
Trappc5

1419421_at
5.609
NM_031158
Ank1
″AFFX-r2-




1424969_s_at
5.604
BC027189
Upp2
Bs-thr-3_s_at″
5.276
AFFX-r2-Bs-


1438793_x_at
5.601
BB222127
1200007D18Rik


thr-3


1458132_at
5.592
BB429249
Cnnm2
1441883_at
5.266
AV092359
0610010O12Rik

1435970_at
5.588
AU035920
Nlk
1455599_at
5.264
AV220135
AI850995

1418389_at
5.585
NM_133703
2810453I06Rik
1450965_at
5.263
BF181445
Tex261

1455281_at
5.581
BB520667
Wdr33
1423048_a_at
5.263
BB400304
Tollip

1422645_at
5.576
AJ306425
Hfe
1444703_at
5.242
BB525928
2810403D21Rik

1420887_a_at
5.569
NM_009743
Bcl2l1
1459871_x_at
5.241
AV349132
2-Mar

1436906_at
5.56
BF781579
Rnf166
1423686_a_at
5.241
BC016234
1110020C13Rik

1434382_at
5.547
AV083936
Tde21
1437857_at
5.234
AV367203
Dpy19l3

1436879_x_at
5.544
AV124668
Afp
1428472_at
5.232
AK003707
Spsb1

1437711_x_at
5.541
BB519474
Odc1 ;
1449762_at
5.231
AV047896





LOC545783 ;
1436897_at
5.227
BE225764
Mfhas1

1419351_a_at
5.226
BC003916
0610007P06Rik
1417677_at
5.037
NM_010098
Opn3

1425330_a_at
5.226
AJ271836
Ppm1b ;
1459896_at
5.036
AW911766
Pogk




LOC433336
1439574_at
5.029
BI737205
1110020A21Rik

1416521_at
5.225
NM_009156
Sepw1
1438549_a_at
5.007
BB020681
Srr

1442711_at
5.222
BB129490
A530065E19Rik
1456467_s_at
5.006
BB389081
Nlk

1452169_a_at
5.221
BC014860
Dgkz
1458662_at
4.999
BB546123
Daam1

1429881_at
5.221
AK018058
Arhgap15
1455052_a_at
4.998
BI153133
2410129H14Rik

1429092_at
5.212
AK009497
Vkorc1l1
1454225_s_at
4.984
AK012700
D3Ertd751e

1436189_at
5.207
AV380561

1421330_at
4.979
NM_019933
Ptpn4

1435516_x_at
5.204
BB752007
Rtel1
1448740_at
4.975
NM_021329
2400006H24Rik

1418234_s_at
5.202
NM_026602
Bcas2
1451395_at
4.972
BC021492
D5Bwg0834e

1434177_at
5.199
AI551117
Ece1
1447097_at
4.966
BG068083


1437137_at
5.197
AV280875
6430550H21Rik
1420010_at
4.966
C80083
LOC574418

1448344_at
5.189
NM_011295
Rps12 ;
1456056_a_at
4.963
AV338866
D6Wsu116e




LOC432865 ;
AFFX-LysX-
4.957
AFFX-LysX-3





LOC546370 ;
3_at







LOC546371 ;
1460203_at
4.95
NM_010585
Itpr1




LOC546372 ;
1429620_at
4.949
BB040330
8430406I07Rik




LOC546373
1442218_at
4.945
BM201230
Map3k9

1451881_at
5.186
BC027044

1417335_at
4.94
NM_017465
Sult2b1

1437489_x_at
5.184
BB497590
Sdhd
1435875_at
4.934
AV223660
Prkab2

1429972_s_at
5.183
BE948556
Txnrd2
1442073_at
4.931
BQ266693


1432579_at
5.177
AA544511
Rshl2
1459696_at
4.928
AI844428
9330186A19Rik

1439150_x_at
5.166
BB480256
Grtp1
1420148_at
4.923
AA589629
Slc6a6

1451596_a_at
5.161
AF068749
Sphk1
1448661_at
4.922
NM_008874
Plcb3

1447397_at
5.158
BB733030
1700129I04Rik
1429005_at
4.919
BB107412
Mfhas1

1434664_at
5.151
BI153133
2410129H14Rik
1429664_at
4.917
AI464135
Cdkl1

1452274_at
5.13
BG976649
Tex27
1424848_at
4.915
U09383
Kcnma1

1422627_a_at
5.121
BF581250
Mkks
1447552_s_at
4.913
AV169798
Catnd2

1422490_at
5.116
AV144704
Bnip2
1455885_at
4.902
AV238106
6530401C20Rik

1418259_a_at
5.112
NM_009849
Entpd2
1451387_s_at
4.894
BC021463
0610039D01Rik

1432343_at
5.111
AK015168
4930419G24Rik
1453993_a_at
4.883
AK014659
Bnip2

1426880_at
5.106
BM250266
BC026657
1453077_a_at
4.882
AK016168
Snapc3

1438769_a_at
5.105
BF719766
MGI:1925112
1451525_at
4.877
BG064038
Arhgap12

1452640_at
5.104
AK014019
3110007F17Rik
1454824_s_at
4.865
BB699957
Mtus1




; LOC546367 ;
1438623_x_at
4.86
AV038578
Rbx1




LOC546368
1436681_x_at
4.857
BE199670
0610009D07Rik

1436475_at
5.103
AI527205
Nr2f2
1440882_at
4.852
BB750940
Lrp8

1435237_at
5.098
AV004950
2310009A05Rik
1435755_at
4.85
BB609468
1110001A16Rik

1419738_a_at
5.094
AK003186
Tpm2
1438656_x_at
4.831
AV157500
Timm17b

1428296_at
5.085
AV102258
Polr2l
1450648_s_at
4.83
NM_010379
H2-Ab1

1418008_at
5.085
BM932567
1810007M14Rik
1439509_at
4.826
BE985592
2900008C10Rik

1436042_at
5.084
BI648366
Tln1
1449012_s_at
4.818
BC027164
Fndc4

1445074_at
5.078
BB519491

1436869_at
4.817
AV304616
Shh

1455601_at
5.072
BB507994

1439388_s_at
4.813
BB251739
Bcar1

1454898_s_at
5.072
AU016407
4833421E05Rik
1428662_a_at
4.809
AK009007
MGI:1916782

1417946_at
5.058
NM_134130
Abhd3
1429988_at
4.804
AK002713
Zfp235

1452768_at
5.058
AK013971
Tex261
1436614_at
4.804
AI843639
AI843639

1434291_a_at
5.054
AA709993
Serf1
1419112_at
4.803
NM_008702
Nlk

1437677_at
5.053
BG063913
AI449595
1449983_a_at
4.799
NM_020282
Nqo2

1428822_a_at
5.053
BB815011
Snx24
1441481_at
4.799
AV262974
Mfap3l

1421083_x_at
5.042
NM_011793
Banf1
1460030_at
4.796
BB355811
Hecw1

1426382_at
5.042
AJ271833
Ppm1b
1457644_s_at
4.785
BB554288
Cxcl1

1437980_at
5.041
BB814947
9130230N09Rik
1440886_at
4.781
BE949427
Cdc37l1

1449471_at
5.04
NM_021452
Kcnmb4
1428309_s_at
4.78
AK003410
Pdrg1

1438408_at
5.039
BB131927
5730467H21Rik
1456036_x_at
4.777
AV003026
Gsto1

1425149_a_at
4.775
BC006578
Pdcl
1453782_at
4.562
BI737125
3021401C12Rik

1419400_at
4.761
AW553649
Mttp
1440603_at
4.555
BB073590


1418996_a_at
4.759
BC021522
4930469P12Rik
1451370_at
4.549
BC027414


1428607_at
4.754
AK010060

1451607_at
4.544
AB039276
Klk21

1423318_at
4.75
AK012795
Rad18
1443073_at
4.543
BB355954
LOC545681

1421295_at
4.749
NM_031258
Chrdl1
1459890_s_at
4.539
C79326
1110008P14Rik

1449027_at
4.747
AF378088
Rhou
1417912_at
4.536
NM_025318
0610009E20Rik

″AFFX-



1438619_x_at
4.533
BB318221
Zdhhc14

PheX-3 at″
4.746
AFFX-PheX-3

1436698_x_at
4.529
AV167328
BC054438

1430203_at
4.746
BG067256
Usp16
1421640_a_at
4.528
NM_011529
Tank

1434732_x_at
4.745
AV044898
Tomm7
1453386_at
4.528
AK008612
Tusc1

1420655_at
4.729
NM_054088
Adpn
1424944_at
4.526
BC024853
Pcp2

1417095_a_at
4.729
NM_015765
Hspa14
1448954_at
4.523
NM_020610
Nrip3

1420556_at
4.728
NM_011025
Oxt
1422153_a_at
4.522
NM_026853
Asb11

1458466_at
4.726
AV338634
Ppgb
1451477_at
4.518
AV047635
LOC545705 ;

1444475_at
4.721
BE983052
1810009N02Rik



LOC545706

1440226_at
4.717
BB088782
BC029103
1437067_at
4.517
BM228625
Phtf2

1440995_at
4.709
BE946005
6430531B16Rik
1430616_at
4.513
BB185833
4930528A17Rik

1430686_at
4.705
AK014719
4833418N02Rik
1434032_at
4.511
BQ175381


1429114_at
4.699
AV276619
Sestd1
1419808_at
4.506
AU022131
Cog4

1454663_at
4.698
BQ176989
Eif5
1428488_at
4.498
BB646655
Pigk

1417590_at
4.693
NM_024264
Cyp27a1
1436926_at
4.491
AV333667
Esrrb

1460648_at
4.684
NM_010150
Nr2f6
1423219_a_at
4.49
BM951442
Mrpl49

1439012_a_at
4.683
BB030204
Dck
1421280_at
4.485
Z36357
Gabra1

1420888_at
4.673
NM_009743
Bcl2l1
1417163_at
4.473
NM_022019
Dusp10

1415956_a_at
4.668
NM_011049
Pctk1
1416132_at
4.467
BB188557
C920006C10Rik

1433700_at
4.667
BB477807
4933433P14Rik
1450770_at
4.465
BC023359
3632451O06Rik

1449534_at
4.661
NM_011517
Sycp3
1456500_at
4.465
AV232784
Aph1b

1435523_s_at
4.656
BB779140
2700089E24Rik
1448586_at
4.461
NM_015765
Hspa14

1456097_a_at
4.653
BB830191
Itgb3bp
1422897_at
4.457
NM_009203
Slc22a12

1434763_at
4.646
BF457736
A730041O15Rik
1451272_a_at
4.455
BC016117
2510010F15Rik

1426035_at
4.646
BC026459
LOC574418
1458638_at
4.451
AI852796
Mtdh

1440663_at
4.634
BB322506
Xpo4
1426496_at
4.447
AK017695
Wdr55

1460553_at
4.623
BF466605
1700025K23Rik
1449752_at
4.445
AI596360
4930422J18Rik

1449021_at
4.618
NM_026308
Rpp21
1428996_at
4.443
AK014774
Gm1705

1437213_at
4.618
BG070110
Nudt21
1440883_at
4.439
BM125576
Usp6nl

1431072_a_at
4.614
BB865814
2610529H08Rik
1422683_at
4.428
NM_022986
Irak1bp1

1438947_x_at
4.613
BB459744
Sema3f
1452291_at
4.426
AV375176
Centd1

1441839_s_at
4.61
BB250824
BC024814
1441960_x_at
4.423
AV267590
5730494M16Rik

1436294_at
4.608
AV337707
Ankrd29
1421230_a_at
4.416
BI696168
Msi2h

1450752_at
4.604
NM_009989
Cyct
1433847_at
4.415
BB098407
D330017J20Rik

1434135_at
4.602
BB107552
B3galnt2
1430521_s_at
4.412
AW548480
Cpne8

1423005_a_at
4.597
NM_019585
Espn
1456727_a_at
4.41
AW413676
Csnk1d

1434777_at
4.596
BG064871
Lmyc1
1433927_at
4.408
AW552571
E430026A01Rik

1423833_a_at
4.592
BC018324
Brp44
1453567_s_at
4.404
AK014997
2810441K11Rik

1456782_at
4.589
BB525604
D1Ertd251e
1424847_at
4.403
M35131
Nefh

1422293_a_at
4.587
NM_134112
Kctd1
1419380_at
4.403
NM_033327
Zfp423

1454119_at
4.587
AK010697
Miknk1
1437810_a_at
4.4
AV311770
Hbb-bh1

1455890_x_at
4.586
BB709603
Smpn ;
1451432_x_at
4.399
BC022729
2010004B12Rik




LOC545062
1446566_at
4.397
BB461266
Ap2b1

1451377_a_at
4.583
BC025501
Aaas
1417740_at
4.394
BB756370
Cdc37l1

1435966_x_at
4.579
BE953095
Mrpl13
1425780_a_at
4.393
BC024352
0610041E09Rik

1418891_a_at
4.577
BB349707
Rab3d
1451998_at
4.391
BC024597
4930485D02Rik

1454102_at
4.57
AK017143
5031425E22Rik
1417035_at
4.391
BI134670
Sac3d1

1433462_a_at
4.564
BB208212
Pi4k2a
1432096_at
4.39
AK010671
Snrpn

1436889_at
4.564
BQ268470
Gabra1
1447927_at
4.388
BG092512
Mpa2l

1457395_at
4.381
AW553048
5330421F07Rik
1457545_at
4.321
BB110572
Shh

1428308_at
4.373
AK003410
Pdrg1
1435956_at
4.317
BB277380
A930004K21Rik

1434249_s_at
4.371
BB083438

1460133_at
4.315
BF607205
Efna5

1423874_at
4.368
BC019463
Wdr33
1437637_at
4.315
BE852433
Phtf2

1424262_at
4.364
BC024599
2810003C17Rik
1452238_at
4.311
BB130716
Hrb

1428568_at
4.363
BB376573
B230217C12Rik
1436060_at
4.31
BB480659
0710005M24Rik

1435510_at
4.363
BB283101
Ppm1h
1452374_at
4.309
BB315154
Zfp322a

1454572_at
4.352
AK013093
2810414N06Rik
1451385_at
4.308
BC010826
2310056P07Rik

1423869_s_at
4.352
AF349659
Txnrd3
1421468_at
4.29
NM_008426
Kcnj3

1451548_at
4.35
BC027189
Upp2
1449557_at
4.289
NM_025904
1600012F09Rik

1418716_at
4.347
AK004037
Mrps25
1421985_a_at
4.288
AI449084
Eif4e2

1426558_x_at
4.334
BB283527
3100002L24Rik
1448699_at
4.286
NM_025317
Mrpl54

1420596_at
4.332
NM_007583
Cacng2
1450095_a_at
4.283
NM_025421
Acyp1

1441453_at
4.328
BB341122
B930096L08Rik
1423495_at
4.272
BE952632
Decr2

1454086_a_at
4.324
AK013416
Lmo2
1427039_at
4.271
AF057285
Epn1

1429087_at
4.323
AK005245
1110054O05Rik
1416407_at
4.269
AI323543
Pea15

1456163_at
4.323
AV127670
2700049P18Rik
1436349_at
4.267
BI408855
2700094K13Rik








































































































































































































































































































































































































































































































































































TABLE 21



Probesets used as negative controls in the comparison of BACarray samples with the reference sample.

GeneSymbol = official gene symbol; ProbeSymbol = Affymetrix probe identification corresponding to gene symbol.

Probesets with an average expression over 100 in the reference sample were plotted.










Gene
Probe
Gene
Probe
Gene
Probe
Gene
Probe

Symbol
Symbol
Symbol
Symbol
Symbol
Symbol
Symbol
Symbol



Cnp1
1418980_a_at
Glul
1426235_a_at
Apoe
1432466_a_at
Slc1a3
1426340_at

Cnp1
1437341_x_at
Glul
1426236_a_at
Apoe
1444108_at
Slc1a3
1426341_at

Cnp1
1449296_a_at
Mbp
1419646_a_at
Laptm5
1417721_s_at
Slc1a3
1439072_at

Cnp1
1460032_at
Mbp
1433532_a_at
Laptm5
1426025_s_at
Slc1a3
1440491_at

Fthfd
1424400_a_at
Mbp
1436201_x_at
Laptm5
1436905_x_at
Slc1a3
1443749_x_at

Fthfd
1424401_at
Mbp
1451961_a_at
Laptm5
1447742_at
Slc1a3
1452031_at

Galc
1420547_at
Mbp
1454651_x_at
Laptm5
1459841_x_at
Vim
1438118_x_at

Galc
1449900_at
Mbp
1456228_x_at
Slc1a2
1439940_at
Vim
1450641_at

Galc
1452907_at
Mog
1448768_at
Slc1a2
1451627_a_at
Vim
1456292_a_at

Gfap
1426508_at
Plp
1425467_a_at
Slc1a2
1457800_at



Gfap
1426509_s_at
Plp
1425468_at
Slc1a2
1458314_at



Gfap
1440142_s_at
Plp
1451718_at
Slc1a2
1459014_at



































Example 13
Physiological Evaluation of TRAP Methodology Results
1) Overview
In order to evaluate the physiological significance of differential expression of genes associated with GABA A receptors in D1 striatonigral of mice treated with cocaine, mice were chronically treated with cocaine or vehicle (saline) and then GABA A receptor function was assayed electrophysiologically.
2) Brain Slice Preparation for Optical/Electrophysiological Study
Slices were obtained from 32- to 37-day-old BAC D1 or BAC D2 soluble eGFP-expressing transgenic mice (S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007)). All animals were handled in accord with Northwestern University ACUC and NIH guidelines. For chronic cocaine studies, mice were injected with 20 mg/kg cocaine or 100 μl saline once daily for fifteen days and brain slices were prepared 4 h after the last injection, as in the mRNA studies. Coronal slices containing the striatum were prepared at a thickness of 250 μm. The mice were deeply anesthetized with ketamine and Xylazine, transcardially perfused with oxygenated, ice cold, artificial cerebral spinal fluid (ACSF), and decapitated. Brains were rapidly removed and sectioned in oxygenated, ice-cold, ACSF using a Leica VT1000S vibratome (Leica Microsystems, Germany). The ACSF contained the following (in mM): 126 NaCl, 3 KCl, 2 CaCl2, 1 MgCl2, 25 NaHCO3, 1.25 NaH2PO4, and 15.6 D-(+)-glucose. Unless otherwise noted, all chemicals and reagents were obtained from Sigma (St. Louis, Mo.). The slices were transferred to a holding chamber in which they were completely submerged in ACSF at 35° C. for 1 h, after which they were stored at room temperature (22° C.-23° C.) until whole-cell recording. All ACSF solutions were bubbled continuously with 95% O2 and 5% CO2 to maintain oxygenation and a ph≈7.4, and periodically checked to ensure ≈300 mOsm/1.
3) Electrophysiological Analysis of GABAergic Synaptic Events
Whole-cell voltage-clamp recordings were performed using standard techniques. Individual slices were transferred to a submersion-style recording chamber on Olympus Optical (Melville, N.Y.) BX50WI microscope and continuously superfused with ACSF at a rate of 2-3 ml/min at 22° C.-23° C. Whole-cell voltage-clamp recordings were performed on striatal medium spiny neurons detected in the slice with the help of an infrared-differential interference contrast (IR-DIC) video microscope with an Olympus OLY-150 camera/controller system (Olympus, Japan). The following were added to the superfusion medium for all experiments to isolate mIPSCs: 50 μM 2-amino-5-phosphonopentanoic acid (AP-5, Tocris Cookson, Ellisville, Mo.) to block NMDA glutamate receptors, 5 μM 1,2,3,4-Tetrahydro-6-nitro2,3-dioxo-benzo quinoxaline-7-sulfonamide (NBQX) to block AMPA/kainite glutamate receptors, and 1 μM tetrodotoxin (TTX, Alomone Labs, Jerusalem, Israel) to block sodium channels. Patch electrodes were made by pulling Sutter BF150-86-10 glass on a P-97 Flaming/Brown micropipette puller (Sutter Instrument, Novato, Calif.) and fire polished before recording. Pipette resistance was typically 2.5-4 MΩ after filling with an internal solution containing the following (in mM): 140 CsCl, 1.5 MgCl2, 10 HEPES, 0.1 BAPTA-Cs, 5 QX-314, 2 ATP-Na2, 0.4 GTP-Na2, pH 7.25-7.3 adjusted with CsOH, 270-280 mOsm/L. Miniature IPSCs were recorded with a Multiclamp 700 A amplifier, a Digidata 1322 A 16-bit data acquisition system, and pClamp software version 8.2 (Molecular Devices, Union City, Calif.) in gap free mode. Neurons were voltage-clamped at −80 mV and allowed to reach a stable baseline (≈5 min) before mIPSCs were recorded for 7 min. Mini Analysis (Synaptosoft Inc., Fort Lee, N.J.) was used to analyze mIPSC amplitude, frequency, 10-90% rise time, decay time, and non-stationary noise analysis. A threshold of 5 times the root mean square baseline noise level (commonly≈20-25 pA) was set for event detection. Records were then visually inspected and all events triggered by noise were discarded. Frequency analysis was carried out on all mIPSCs that met threshold criteria. Events were then selected for amplitude, 10-90% rise time, and decay time analysis on the following criteria: 1) Events with 10-90% rise times faster than 1 ms were selected to minimize space clamp errors and electrotonic filtering; 2) Events with decay times faster than 50 ms were selected to minimize events in which multiple events precluded accurate decay time measurement. For non-stationary noise analysis, the same criteria for amplitude and mIPSCs kinetics were used followed by these additional criteria: 3) No multiple events in which the decay did not return to baseline; 4) Events had to have a stable baseline before the rise and after the end of the decay. Mean values of mIPSC measures were compared between groups with a t-test or Mann-Whitney rank sum test using Sigma Stat 3.0 (Systat Software Inc., Richmond, Calif.). Pooled data are presented as means±SE and box plots using IGOR 5.00 (WaveMetrics, Lake Oswego, Oreg.).
4) 2-Photon Laser Scanning Microscopy (2PLSM)
Striatonigral (BAC D1) or striatopallidal (BAC D2) neurons expressing soluble eGFP (S. Falcon, R. Gentleman, Bioinformatics 23, 257-8 (2007)) in 275 μm thick corticostriatal slices were identified by somatic eGFP fluorescence. eGFP 2PLSM green signals (500-550 nm) were acquired from eGFP+ BAC Drd2 neurons using 810 nm excitation, while eGFP+ BAC Drd1 neurons required 900 nm excitation. eGFP+ MSNs were then patched using infrared-differential interference contrast (IR-DIC) video microscopy with a Hamamatsu C2400 Newvicon camera/controller system (Hamamatsu, Japan) and a 60X/0.9NA water-dipping lens. Patch electrodes were made by pulling BF150-86-10 glass on a P-97 Flaming/Brown micropipette puller (both from Sutter Instrument Co., Novato, Calif.). The pipette solution contained the following (in mM): 135 KMeSO4 (ICN Biomedicals Inc., Aurora, Ohio), 5 KCl, 10 HEPES, 2 MgATP, 0.2 Na2GTP, and 0.1 spermine, pH=7.25-7.3 with KOH, 270 mOsm/l. As measured in the bath, the pipette resistance was ˜4 MΩ. Seals were formed in voltage-clamp mode on the cell somas with series resistance >1 GΩ. After seal rupture in whole-cell configuration the series-resistance decreased to 10-15 MΩ. Alexa 594 (50 μM, Ca 2+ -insensitive red dye), for visualization of the dendrites, and Fluo-4 (200 μM, Ca 2+ -sensitive green dye), for measuring changes in intracellular dendritic Ca 2+ , was dissolved in the internal pipette solution. eGFP fluorescence was below the threshold for detection in the dendrites and did not contribute background signal during calcium imaging, Thapsigargin was also added to the internal solution in some experiments to block ER-mediated Ca 2+ release. The thapsigargin was initially dissolved in DMSO at a concentration of 10 mM. This stock was then diluted 1000× in the internal recording solution. Following break in, the internal solution was allowed to approach diffusional equilibrium for at least 15 min prior to imaging The cells were voltage clamped at −70 mV and monitored for somatic depolarization.
For the duration of the recordings, 2PLSM green and red signals (570-620 nm) were acquired using 810 nm excitation with 90 MHz pulse repetition frequency and ˜250 fs pulse duration at the sample plane. A second puffer pipette containing sphingosine 1-phosphate (S1P, 10 μM) was positioned for focal dendritic application. The S1P was initially dissolved in DMSO at a concentration of 10 mM. This stock was then diluted 1000× into HEPES-buffered ACSF containing 4 mg/ml BSA, pH=7.4. Changes in dendritic Ca 2+ were measured by acquiring high magnification maximum projection images of dendrite segments 50-100 μm from the soma. These images were acquired with 0.17 μm 2 pixels and 2.2 μs dwell time and consisted of 10-20 images taken at 0.5 μm focal steps. The change in Ca 2+ was determined by calculating the percent change in fluorescence of the green dye normalized to the red dye (ΔG/R). Following Ca 2+ imaging, maximum projection images of the soma and dendritic field were acquired with 0.27 μm 2 pixels and 2.6 μs pixel dwell time and consisted of ˜80 images taken at 0.7 μm focal steps.
The two-photon excitation source was a Chameleon-XR tunable laser system (705 nm to 980 nm, Coherent Laser Group, Santa Clara, Calif.). Laser average power attenuation was achieved with two Pockels cell electro-optic modulators (models 350-80 and 350-50, Con Optics, Danbury, Conn.). The two cells are aligned in series to provide enhanced modulation range for fine control of the excitation dose (0.1% steps over four decades). The laser-scanned images were acquired with a Bio-Rad Radiance MPD system (Hemel Hempstead, England, UK). The fluorescence emission was collected by external or non-de-scanned photomultiplier tubes (PMTs). The green fluorescence (500 to 550 nm) was detected by a bialkali-cathode PMT and the red fluorescence (570 nm to 620 nm) was collected by a multi-alkali-cathode (S20) PMT. The fluorescence was acquired in 16 bit photon counting mode. The laser light transmitted through the sample was collected by the condenser lens and sent to another PMT to provide a bright-field transmission image in registration with the fluorescent images. The stimulation, display, and analysis software was a custom-written shareware package, WinFluor and PicViewer (John Dempster, Strathclyde University, Glasgow, Scotland; UK).
5) Evaluation of Differential GABA A Receptor Function in D1 and D2 Following Chronic Cocaine Treatment
Cocaine treatment increased the frequency of GABA A receptor miniature inhibitory postsynaptic currents (mIPSCs) in striatonigral neurons as presented in the following figures.
FIG. 31 showed that cocaine treatment increased the frequency of small amplitude GABAergic mIPSCs in BAC D1 striatonigral neurons. (a) Representative spontaneous mIPSCs traces from BAC D1 striatonigral neurons (expressing soluble eGFP under the D1 promoter) taken from mice treated for 15 days with saline or (b) cocaine (20 mg/kg/day). (c) Bar graph summary of mean mIPSC frequency showing a increase in BAC D1 striatonigral neuron mIPSCs frequency following cocaine treatment (Mann-Whitney Rank Sum Test, p<0.05, saline median=0.82 Hz, n=22; cocaine median=1.03 Hz, n=26). (d) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) increased in BAC D1 striatonigral neurons following cocaine treatment (t-test, p<0.05, saline=79.4±8.2, n=22; cocaine=104.2±6.3, n=26). (e) Representative variance-mean current plots from saline treated and cocaine treated BAC D1 neurons suggesting that the cocaine-induced small amplitude events arise from synapses that have fewer GABAA receptors (N) per synapse but receptors with an unchanged unitary receptor conductance (g) (saline N=33, g=3 1 pS; cocaine N=27, g=30 pS). (f) Representative spontaneous mIPSCs traces from BAC D2 striatopallidal neurons following saline treatment for 15 days and (g) following cocaine treatment for 15 days. (h) Bar graph summary of mean mIPSC frequency in saline and cocaine treated neurons, showing no effect of treatment condition (t-test, p>0.05, saline=0.72±0.05 Hz, n=12; cocaine=0.78±0.07 Hz, n=16). (i) Bar graph summary showing that the number of small amplitude mIPSCs (<75 pA) in equal length records (7 min) was not altered by treatment condition in BAC D2 neurons (t-test, p>0.05, saline=68.1±9.7, n=12; cocaine=71.7±7.5, n=16). (j) Representative variance-mean current plots showing that cocaine treatment did not change in the number of receptors per synapse or the unitary receptor conductance in BAC D2 neurons (saline N=29, g=33 pS; cocaine N=31, g=29 pS).
In summary, FIG. 32 showed that cocaine treatment decreased the number of GABAA channels per synapse in BAC D1 striatonigral neurons. Box plot summaries showing mean mIPSCs kinetics and non-stationary noise analysis measures of individual striatonigral neurons taken from saline-treated control and 15-day treated cocaine BAC D1 mice. (a) Neither mean 10-90% rise times (t-test, p>0.05, Saline=0.64±0.008, n=22; Cocaine=0.65±0.006, n=26) nor (b) mean decay times (ttest, p>0.05, Saline=17.3±0.5, n=22; Cocaine=17.0±0.5, n=26) were different between groups. Non-stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (g) of GABAA receptors of striatonigral neurons (t-test, p>0.05, Saline g=31.1±1.6, n=22; Cocaine g=30.8±1.2, n=26), and a decrease in the (d) mean number of channels per synapse in striatonigral neurons taken from cocaine treated BAC D1 mice (t-test, p<0.05, Saline N=33.1±1.4, n=22; Cocaine N=29.3±1.0, n=26).
In summary, FIG. 33 showed that cocaine treatment did not alter GABAergic mIPSCs kinetics or GABAA receptor number per synapse or unitary receptor conductance in BAC D2 striatopallidal neurons. Box plot summaries showing mean mIPSCs kinetics and non-stationary noise analysis measures of individual striatopallidal neurons taken from saline-treated control and 15-day treated cocaine BAC D2. (a) Neither mean 10-90% rise times (t-test, p>0.05, Saline=0.65±0.007, n=12; Cocaine=0.64±0.010, n=16) nor (b) mean decay times (t-test, p>0.05, Saline=18.0±1.0, n=12; Cocaine=18.0±0.5, n=16) were different between groups. Non-stationary noise analysis of mIPSCs demonstrated no change between groups in the (c) mean unitary conductance (g) of GABAA receptors of striatopallidal neurons (t-test, p>0.05, Saline g=30.7±1.5, n=12; Cocaine g=30.3±1.2, n=16) or in the (d) mean number of channels per synapse in striatopallidal neurons taken from cocaine treated BAC D2 mice (t-test, p>0.05, Saline N=33.8±2.7, n=12; Cocaine N=33.0±1.6, n=16).
Example 14
Molecular Phenotyping and Translational Profiling of Cerebellar Cells
1) Overview
The cerebellum is a region of the brain that plays a role in the integration of sensory perception and motor control. In order to coordinate motor control, there are several neural pathways linking the cerebellum with the cerebral motor cortex (which sends information to the muscles causing them to move) and the spinocerebellar tract (which provides proprioceptive feedback on the position of the body in space). The cerebellum integrates these pathways using constant feedback on body position to fine-tune motor movements (Fine E J, Ionita C C, Lohr L (2002). “The history of the development of the cerebellar examination”. Semin Neurol 22 (4): 375-84. doi:10.1055/s-2002-36759). Studies show that the cerebellum also serves other functions, such as cognitive functions, including attention and the processing of language, music, and other sensory temporal stimuli (Rapp, Brenda (2001). The Handbook of Cognitive Neuropsychology: What Deficits Reveal about the Human Mind. Psychology Press, 481).
Damage to the cerebellum typically is not so debilitating as to cause paralysis, but rather presents as feedback deficits resulting in disorders in fine movement, equilibrium, posture, and motor learning. Ataxia is a complex of symptoms, generally involving a lack of coordination, that is often found in disease processes affecting the cerebellum. To identify cerebellar problems, the neurological examination includes assessment of gait (a broad-based gait being indicative of ataxia), finger-pointing tests and assessment of posture. Structural abnormalities of the cerebellum (hemorrhage, infarction, neoplasm, degeneration) may be identified on cross-sectional imaging. Magnetic resonance imaging is the modality of choice, as computed tomography is insufficiently sensitive for detecting structural abnormalities of the cerebellum (Gilman S (1998). “Imaging the brain. Second of two parts”. N. Engl. J. Med. 338 (13): 889-96).
2) BACarray Mice Expressing eGFP in Individual Cerebellar Cell Types
There are multiple cell layers and several cerebellar cell types. The innermost granular layer contains the cell bodies three types of cells: the numerous and tiny granule cells, the larger Golgi cells and the medium sized neuronal unipolar brush cells. The middle Purkinje layer contains only one type neuron, the Purkinje cell. Purkinje cells are the primary integrative neurons of the cerebellar cortex and provide its sole output. Bergmann glia, a type of glia also known as radial epithelial cells are astrocytes in the cerebellum that have their cell bodies in the Purkinje cell layer and processes that extend into the molecular layer, terminating with bulbous endfeet at the pial surface (Eccles J. C Ito, M and Szentagothai J. (1967). “The cerebellum as a neuronal machine”. Springer Verlag; Llinás, R., Baker, R. and Sotelo, C. (1974). “Electrotonic coupling between neurons in cat inferior olive”. J. Neurophysiol 37: 560-571). The outermost layer of the cerebellar cortex contains two types of inhibitory interneurons: the stellate and basket cells. It also contains the dendritic arbors of Purkinje neurons and parallel fiber tracts from the granule cells. BACarray mice with specific expression patterns in specific cerebellar cell types can be created: expression of tagged ribosomal protein under the control of a regulatory region from the Neurod1 loci to label cerebellar granule cells; expression of tagged ribosomal protein under the control of a regulatory region from the Grm2 loci to label cerebellar inner golgi neurons; expression of tagged ribosomal protein under the control of a regulatory region from the Grp loci to label unipolar brush cells; expression of tagged ribosomal protein under the control of a regulatory region from the Pttg3 loci to label cerebellar astrocytes; and expression of tagged ribosomal protein under the control of a regulatory region from the Lypd6 loci to label stellate, basked cells.
3) Translational Profiling and Molecular Phenotyping of Cells from AT Mice
Ataxia telangiectasia (AT) is a disease whose principle features includes ataxia, development of lymphocytic tumors and cerebellar degeneration (P. J. McKinnon, EMBO Rep 5, 772 (2004); F. Gumy-Pause, P. Wacker, A. P. Sappino, Leukemia 18, 238 (2004); L. Farina et al., J Comput Assist Tomogr 18, 724 (1994); F. Tavani et al., Neuroradiology 45, 315 (2003)). AT is caused by recessive loss of function mutations in the gene encoding ataxia telangiectasia mutated (ATM), a ubiquitously expressed nuclear protein kinase involved in the DNA damage response and cell cycle control (K. Savitsky et al., Science 268, 1749 (1995); R. T. Abraham, Genes Dev 15, 2177 (2001); Y. Shiloh, Nat Rev Cancer 3, 155 (2003); M. F. Lavin, S. Kozlov, Cell Cycle 6, 931 (2007); S. Matsuoka et al., Science 316, 1160 (2007)). Atm knockout mice display many of the characteristics of the human disorder, including sensitivity to irradiation and lymphocytic tumor formation (C. Barlow, K. D. Brown, C. X. Deng, D. A. Tagle, A. Wynshaw-Boris, Nat Genet 17, 453 (1997); K. H. Herzog, M. J. Chong, M. Kapsetaki, J. I. Morgan, P. J. McKinnon, Science 280, 1089 (1998); A. Elson et al., Proc Natl Acad Sci USA 93, 13084 (1996); Y. Xu et al., Genes Dev 10, 2411 (1996); P. R. Borghesani et al., Proc Natl Acad Sci USA 97, 3336 (2000)). However, one of the distinguishing features of AT, the degeneration of cerebellar Purkinje cells, does not occur in the mouse model of the disease. Since clinical evidence of substantial Purkinje cell loss in AT patients is not seen until affected children reach several years of age (R. A. Gatti et al., Clin Rev Allergy Immunol 20, 87 (2001)), and since the average lifespan of a mouse is less than two years, it is possible that the pathogenic process simply does not have enough time to result in Purkinje cell degeneration in the AT mouse model. In one embodiment, ribosomes in specific cerebellar cell types in a mouse model of AT can be molecularly labeled (Doyle et al., submitted).
Molecular phenotyping and translational profiling of Purkinje cells in Atm knockout mice, hereafter Atm−/− mice, could reveal changes in response to the mutation. This enables the identification of genes that are differentially regulated in a cell-type specific manner, by employing the BACarray strategy and TRAP methods. Changes in response to the Atm mutation were analyzed in Purkinje cells and Bergmann glia, a specialized glial cell population with similar abundance and anatomical distribution as Purkinje cells.
Transgenic BACarray mice expressing the eGFP-L10a fusion protein were prepared using the Purkinje cell protein 2 (Pcp2) and Septin 4 (Sept4) BAC vectors described by the GENSAT project (www.gensat.org; S. Gong et al., Nature 425, 917 (2003)) ( FIG. 34 ). BAC transgenic animals were generated using modified BAC constructs as previously described (J. Zahringer, B. S. Baliga, H. N. Munro, Proc Natl Acad Sci USA 73, 857-61 1976)) using either the Pcp2 gene or the Septin 4 gene as drivers for the ribosomal protein L10 enhanced green fluorescent protein (eGFP L10a) construct in the Purkinje cells, and Bergmann glia, respectively. In other embodiments, other drivers of cell-type specific gene expression could be used, and is in no means meant to be limiting. Transgenic lines were generated and genotyping confirmed using conventional techniques. Pcp2 and Sept4 BACarray transgenic animals were then crossed with Atm+/animals After two generations, the Pcp2 and Sept4 BACarray constructs could be recovered in Atm+/+ or Atm−/− animals Heterozygote Atm+/littermates were bred to generate wildtype+/+ and knockout animals Immunohistochemical and double immunofluorescence staining of sections from the BACarray mice is presented in FIG. 35 . Panel A demonstrated the expression of the eGFP-L10a fusion specifically in the soma and primary dendrites of cerebellar Purkinje cells in the Pcp2 line, and panel B demonstrated its expression in Bergmann glia in the Sept4 line.
To ensure that Purkinje cell and Bergmann glial cell mRNAs could be enriched from the Pcp2 and Sept4 BACarray transgenic lines, microarray data were collected from each of the BACarray lines in triplicate by affinity purification of eGFP-L10a containing polysomes. In each case, the levels of expression of immuno-precipitated (IP) mRNAs were compared to their expression levels in the unbound fraction to determine their enrichment in Purkinje cells or Bergmann glia. Scatter plots ( FIG. 36 ) of these data revealed that thousands of mRNAs could be enriched by affinity purification of polysomes from either the Pcp2 or Sept 4 BACarray cerebella. As expected, known Purkinje cell specific mRNAs were specifically enriched in IP samples from the Pcp2 BACarray mice (
Table 22), and known Bergmann glial enriched mRNAs were specifically enriched in the IP samples from the Sept4 BACarray mice (Table 23). The data establish translational profiles and molecular phenotypes of these two cerebellar cell types.
Microarray data were imported into Genespring GX 7.3.1 (Agilent Technologies, Santa Clara, Calif.) as GeneChip CEL files. For each experiment, Atm+/+ and Atm−/− data sets were imported together and normalized using GC RMA. For total cerebellum experiments, four replicates for each condition (Atm+/+ or Atm−/−) were used, for the Septin4 experiments, five replicates for each condition were used, and for the Pcp2 experiments, six replicates were used for each condition. Once imported, experiments were viewed as scatter plots for further analysis. For PCP2 and Septin 4 experiments, Atm+/+ and Atm−/− data were further normalized per chip using the Affymetrix hybridization controls as positive control genes and divided by the constant value of 0.001. In order to generate lists of regulated genes, data were filtered on expression with a cutoff of 25 and filtered on fold change with a cutoff of 1.5. One-way ANOVA analysis was used to determine statistical significance with a p value of ≤0.05.





TABLE 22



Purkinje Cell Positive Control Genes.










Fold Change,
Fold Change,

Gene Symbol
GenBank Accession
Affymetrix ID
PCP2 IP vs. Unb
Septin4 IP vs. Unb









Slc1a6
NM_009200
1418933_at
14.76
0.126

Kcnip1
NM_027398
1416785_at
12.26
0.627

Pcp4l1
AV337888
1452913_at
11.59
0.959

Car8
BC010773
1424958_at
11.37
0.0251

Cdr2
NM_007672
1417430_at
10.23
0.0651

Trpc3
NM_019510
1417577_at
9.951
0.0976

Kcnip1
NM_027398
1448459_at
9.694
0.456

Pcp2
NM_008790
1419084_a_at
9.553
0.173

Kitl
BB815530
1448117_at
9.02
0.599

Kitl
M64262
1426152_a_at
7.551
1.116

Plxdc1
AF378760
1424902_at
6.91
0.0552

Kitl
BB815530
1415855_at
6.547
1.077

Pcp2
NM_008790
1419085_at
6.21
0.287

Grid2ip
NM_133355
1450310_at
5.514
0.414

Calb1
BB246032
1448738_at
4.769
0.0384

Thy1
AV028402
1423135_at
4.488
0.167

Kitl
AW551461
1440621_at
3.836
0.985

Dab1
NM_010014
1421100_a_at
3.085
0.346

Car8
X61397
1427482_a_at
2.984
0.0834

Pcp4
NM_008791
1460214_at
2.434
0.642

Dab1
BB644109
1427308_at
2.388
0.151

Calb1
BB246032
1417504_at
1.824
0.0755

Grid2
BG075811
1459245_s_at
1.816
1.032

Kcnip1
AK013657
1454504_at
1.752
1.223

Dab1
BB429377
1435577_at
1.659
0.122

Slc1a6
NM_009200
1418933_at
14.76
0.126

Dab1
BB644109
1427307_a_at
1.529
0.774



Genes known to be specifically expressed in Purkinje cells were analyzed by scatter plot analyses with data from both Pcp2 and Septin4 lines. These genes are enriched in the Pcp2 IP data relative to total cerebellum (Unb), whereas they are underrepresented in the Septin4 IP data relative to total cerebellum (Unb).























































TABLE 23



Bergmann Glial Cell Positive Control Genes










Fold Change,
Fold Change,

Gene Symbol
GenBank Accession
Affymetrix ID
Septin4 IPvsUnb
PCP2 IPvsUnb









Vim
AV147875
1456292_a_at
20.41
0.0313

Vim
M24849
1450641_at
13.29
0.053

Mlc1
NM_133241
1448139_at
13.07
0.0218

Slc1a3
BB357585
1426340_at
12.85
0.0635

Phgdh
L21027
1426657_s_at
12.32
0.106

Vim
AV147875
1438118_x_at
12.31
0.0556

Slc1a3
BB357585
1426341_at
11.79
0.268


NM_009115
1419383_at
10.77
0.039

4-Sep
AW208509
1455422_x_at
10.48
0.415

Slc1a3
BB357585
1452031_at
9.359
0.0409

Fabp7
NM_021272
1450779_at
8.724
0.0966

Phgdh
AV216768
1437621_x_at
8.696
0.0343

4-Sep
NM_011129
1448729_a_at
8.277
0.474

Ednrb
BF100813
1437347_at
7.807
0.103

Slc1a3
BB308757
1439072_at
7.424
0.537

Slc1a3
BB085413
1443749_x_at
6.165
0.102

Gfap
BB183081
1426508_at
6.075
1.031

Gsta4
NM_010357
1416368_at
5.171
0.0499

Ednrb
BB451714
1423594_a_at
5.026
0.153

Gfap
BB183081
1426509_s_at
4.77
0.408

Gfap
BB750040
1440142_s_at
4.311
0.0098

Ednrb
BB770914
1426314_at
3.959
0.651

Phgdh
BB495884
1456584_x_at
3.039
0.105

Phgdh
L21027
1426658_x_at
2.927
0.997

Mthfd1
AV215673
1436704_x_at
2.072
0.663

Phgdhl1
BC024467
1423928_at
2.006
0.576

Phgdhl1
BC024467
1423929_at
1.844
1.363

Nes
AI413223
1449022_at
1.794
0.732

Mthfd1
NM_138745
1415917_at
1.786
0.759

Mthfd1
NM_138745
1415916_a_at
1.773
0.32



Genes known to be expressed in Bergmann glial cells were analyzed by scatter plot analyses with data from both Septin4 and Pcp2 lines. These genes are robustly enriched in the Septin4 IP data relative to data from total cerebellum (Unb). Conversely, these genes are underrepresented in data from Pcp2 IP relative to total cerebellum (Unb).





















































4) Loss of ATM Function
Pcp2 and Sept4 BACarray transgenic animals were then crossed with Atm+/− animals. After two generations, the Pcp2 and Sept4 BACarray constructs could be recovered in Atm+/+ or Atm−/− animals. To determine whether Purkinje cells respond differently than do Bergmann glia to loss of ATM function, microarray data were collected from total cerebellum, from Purkinje cells and from Bergmann glial cells using 6-8 week old Pcp2 and Sept4 wt and Atm−/− BACarray mice. Lists of genes regulated in Atm−/− animals were generated by identifying all genes with expression values greater than 25 and at least a 1.5 fold difference in expression between wt and Atm−/− animals. One-way ANOVA analysis with p-value cutoff of 0.05 was used to select for significance. Scatter plot and Venn diagram analyses were used to identify genes whose expression changed in response to the Atm mutation in whole cerebellum, Purkinje cells or Bergmann glia ( FIG. 37 ). Data for individual genes are presented in Table 24-Table 29.
The majority of genes whose expression is altered in Purkinje cells of Atm−/− mice (65/89) can not be detected by microarray analysis of total cerebellar mRNA. Moreover, only 17 of the 89 mRNAs whose expression changes in Purkinje cells in Atm−/− animals are also changed in Bergmann glial cells. Finally, the levels of expression of 94 genes are altered specifically in Atm−/− Bergmann glia versus Purkinje cells. The responses of specific cell types to the Atm mutation are largely distinct, and that translational profiling and molecular phenotyping of specific cell types using the BACarray strategy can reveal responses that can not be observed by analysis of histologically complex tissues.





TABLE 24



Genes Upregulated in Atm−/− Total Cerebellum Data






Gene Symbol
GenBank Accession
Affymetrix ID
Fold Change








AI451250
AI451250
1447436_at
21.3

Scn2b
AK013326
1430648_at
16.86

Nedd4
NM_010890
1421955_a_at
6.112

2010007H06Rik
BB483585
1446748_at
5.587

MGI:2136405
BB272589
1440386_at
3.752

C920006O11Rik
BB552195
1435704_at
3.486

Ddx6
BC021452
1424598_at
3.371

Opcml
BB383296
1457446_at
2.273

Cyp11a1
C87524
1439947_at
2.113

Btbd15
BC027138
1427271_at
2.048

Mod1
BC011081
1416632_at
2.032

Cgnl1
BB409331
1452309_at
1.941

Loh11cr2a
BC004727
1426221_at
1.914

Arcn1
BB757123
1436062_at
1.829

AI835735
AI835735
1459716_at
1.817

Map2k6
BB261602
1426850_a_at
1.783

Nedd4
BG073415
1451109_a_at
1.757

Grid2
NM_008167
1421435_at
1.749

Hif3a
AF416641
1425428_at
1.747

Arid5b
BB699910
1420973_at
1.747

LOC546041
AI585679
1436740_at
1.744

Thy1
AV028402
1423135_at
1.727

AI593442
BF658761
1444026_at
1.697

Usp2
AI553394
1417168_a_at
1.697

Enpp3
BB039510
1439260_a_at
1.693

Cdkn1a
AK007630
1424638_at
1.688

Tspan18
AK011742
1429856_at
1.665

Mecr
NM_025297
1417097_at
1.656

D13Ertd787e
AU020421
1420098_s_at
1.645

Bcl6
U41465
1421818_at
1.644

Arid5b
BB079486
1434283_at
1.64

A730090H04Rik
AV251751
1445913_at
1.63

Sgk
NM_011361
1416041_at
1.628

1500041N16Rik
NM_026399
1418732_s_at
1.602

Dlat
AV336908
1426265_x_at
1.589

A430108E01Rik
AW541326
1438049_at
1.584

Hif3a
BB486740
1446708_at
1.573

Syt13
BE648447
1451045_at
1.564

Mtap6
BM233608
1457316_at
1.555

Cald1
AK014755
1433147_at
1.549

Elmo1
BC024727
1424523_at
1.547

Arf2
AV023312
1438661_a_at
1.544

2610005L07Rik
BB471300
1437717_x_at
1.534

AI449310
AW552116
1455451_at
1.523

Scn2b
BQ175340
1436134_at
1.522

Mvp
NM_080638
1448618_at
1.521

Pcdha11
BB265776
1451769_s_at
1.518

Txnrd3
AI196535
1449623_at
1.516

Frmd6
BC019939
1451264_at
1.513

4732464A07Rik
BB004948
1459951_at
1.51


BB627097
1442019_at
1.509

8430436O14Rik
AK018466
1452761_a_at
1.508



Genes which are upregulated in Atm−/− cerebellum relative to Atm+/+ cerebellum. Microarray data from Atm+/+ and Atm−/− cerebellum were compared in scatter plots, and filtered on expression of at least 25 and on fold change of at least 1.5, with Atm−/− values greater than Atm+/+. Enriched genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.















































































TABLE 25



Genes Downregulated in Atm−/− Total Cerebellum Data






Gene Symbol
GenBank Accession
Affymetrix ID
Fold Change








Tmod3
AK017725
1423088_at
0.66

Leo1
BG065311
1455293_at
0.658

Sirt4
AK016400
1426847_at
0.656

Kcna6
AV328356
1441049_at
0.656


BG066504
1435998_at
0.655

Osbpl6
AI851164
1449627_at
0.654

Cryab
AV016515
1434369_a_at
0.653

Malat1
AW012617
1427285_s_at
0.646

Ddx42
AW763628
1443719_x_at
0.642


AV372127
1438754_at
0.639

6430514L14Rik
BC025646
1425213_at
0.637

Smarce1
AK018493
1422675_at
0.632

Dnaja4
BQ176119
1434196_at
0.63

1700047I17Rik
BB516625
1445334_at
0.629

2010309E21Rik
BF017486
1441796_at
0.629

Htr2c
BQ174268
1435513_at
0.625

Dpy19l1
BE949451
1433759_at
0.615

Gpr153
AK004377
1426973_at
0.613

Ncam2
AF001287
1425301_at
0.602

2310004I03Rik
BM939621
1428698_at
0.598

Ier2
NM_010499
1416442_at
0.597

Cd80
BQ175573
1440592_at
0.591

Vps11
BB322416
1441922_x_at
0.591

1110037N09Rik
BG143502
1438216_at
0.588

Gcl
BM239632
1438888_at
0.586

Nrip1
BB041915
1454295_at
0.586

Tmod2
AK018223
1430153_at
0.582

Rfxdc2
BB148972
1460567_at
0.577

A130022J15Rik
BI149851
1433671_at
0.577

Pou3f2
BE991235
1457734_at
0.567

Tmem25
BB245618
1436644_x_at
0.562

Phf20l1
BB268102
1454939_at
0.556

Mcam
NM_023061
1416357_a_at
0.552

Aph1c; Aph1b
AK002310
1429466_s_at
0.543

Cryab
NM_009964
1416455_a_at
0.541

Fmr1
AF170530
1452550_a_at
0.531

B3galt1
AV328619
1441396_at
0.516

4833427G06Rik
AK014780
1430762_at
0.496

Pts
NM_011220
1450660_at
0.467

BC027092
BB445523
1435676_at
0.43

BC034204
BB830346
1433661_at
0.425

BC038167
BB211614
1458104_a_at
0.422

Ptch2
BB530125
1457256_x_at
0.415

Dixdc1
BC024834
1425256_a_at
0.415

Fxyd6
AB032010
1417343_at
0.369

Bace1
AF200346
1421825_at
0.356

Pdgfd
BB428671
1456532_at
0.34

Lrrc49
BB484412
1447954_at
0.327

Tipin
BB796715
1459720_x_at
0.277

C030014I23Rik
AK021081
1453044_at
0.265

2310030G06Rik
NM_025865
1449357_at
0.238

Rps25
BM729504
1430978_at
0.129

Ppcdc
BB329391
1424336_at
0.103



Genes which are downregulated in Atm−/− cerebellum relative to Atm+/+ cerebellum. Microarray data from Atm+/+ and Atm−/− cerebellum were compared in scatter plots, and filtered on expression values of at least 25 and selected for genes with wt values at least 1.5 fold greater than Atm−/− values. Differential genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.
















































































TABLE 26



Genes Upregulated in Atm−/− Septin4 IP Data






Gene Symbol
GenBank Accession
Affymetrix ID
Fold Change








2010007H06Rik
BB483585
1446748_at
14.16

Nedd4
NM_010890
1421955_a_at
4.926

Bmp5
AV032115
1455851_at
4.228

C920006O11Rik
BB552195
1435704_at
3.964

Scn2b
AK013326
1430648_at
3.629

AI451250
AI451250
1447436_at
3.451

Ddx6
BC021452
1424598_at
2.672

Bmp5
NM_007555
1421282_at
2.57

Bmp5
NM_007555
1421283_at
2.44

Ccl27
NM_011336
1419188_s_at
2.179


BB520860
1458145_at
2.178

Tle1
NM_011599
1422751_at
1.973

Loh11cr2a
BC004727
1426221_at
1.957

Kcna4
BB131475
1438613_at
1.955

Mod1
BC011081
1416632_at
1.942

MGI:2136405
BB272589
1440386_at
1.866


AI467657
1442025_a_at
1.864

Cebpd
BB831146
1423233_at
1.846

Btbd15
BC027138
1427271_at
1.777

Tpm1
BM232388
1456623_at
1.767

Wee1
NM_009516
1416773_at
1.621

D15Ertd366e
AV114522
1450629_at
1.579

4832420M10
BB012037
1440990_at
1.57

Hhip
NM_020259
1421426_at
1.547

4930466F19Rik
AV044111
1432648_at
1.541

Il11ra1; Il11ra2
BC004619
1417505_s_at
1.52

MGI:1921571
AK011357
1426612_at
1.512



Genes which are upregulated in Atm−/− relative to Atm+/+ in the Septin4 experiments. Microarray data from Atm+/+ and Atm−/− Septin4 IPs were compared in scatter plots, and filtered on expression of at least 25 and with Atm−/− values at least 1.5 fold greater than Atm+/+. Enriched genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.






















































TABLE 27



Genes Downregulated in Atm−/− Septin4 Data






Gene Symbol
GenBank Accession
Affymetrix ID
Fold Change








Aak1
AW121504
1441782_at
0.667


AI503515
1437979_at
0.666

Nmnat1
AF260924
1425773_s_at
0.663


BM940493
1445699_at
0.663

Dpy19l1
BE949451
1433759_at
0.663

Jmjd3
BB494168
1447612_x_at
0.662

Zfyve20
BC017622
1429517_at
0.662

2410076I21Rik
AV216663
1441957_x_at
0.66

Tln2
AV270892
1429111_at
0.657

Hdlbp
C77256
1449615_s_at
0.657

Fbxo5
AK011820
1429499_at
0.657

Ddc
AF071068
1426215_at
0.656

Hmg20a
AI987819
1428920_at
0.649

Pcdhb12
NM_053137
1422877_at
0.649

Zcchc3
AV140894
1428401_at
0.647

Vps11
AK004695
1428110_x_at
0.647

Itsn1
AA172344
1452338_s_at
0.646

LOC545039
BB099075
1440815_x_at
0.646

2810452K22Rik
AK007812
1454253_at
0.644

Zdhhc24
BB271151
1428278_at
0.644


BB354954
1444359_at
0.644

Sstr3
BQ174132
1441603_at
0.641

4933427G17Rik
AK016951
1431905_s_at
0.638

Calml4
AY061807
1424713_at
0.637

Tbl3
BC019504
1415750_at
0.636

A830053O21Rik
BB271275
1446501_at
0.635

Car7
BB193643
1443824_s_at
0.634

Zfp316
AV367169
1444528_at
0.63

4833424O15Rik
BB346520
1429249_at
0.629

2310076O21Rik
BC017618
1451304_at
0.627

Dusp16
BB121278
1440615_at
0.627

Tgoln1
AI314055
1423308_at
0.625

Sv2a
BC026494
1423406_at
0.625

Map3k10
BG070411
1440500_at
0.624

Foxp1
BM220880
1435221_at
0.623

Bace1
AF200346
1421825_at
0.623

Nr2f2
AI527205
1436475_at
0.619


AV028487
1457337_at
0.617

1110025F24Rik
AK012340
1453562_a_at
0.617


AV023306
1438395_at
0.617

8430427H17Rik
AK018446
1428284_at
0.616

Cops3
BB283617
1443570_at
0.616

4930506M07Rik
BB559293
1429055_at
0.614

Larp6
NM_026235
1418400_at
0.609

Lzp-s
AV058500
1439426_x_at
0.609


BQ175164
1441091_at
0.607

C230004F18Rik
BB380166
1445854_at
0.607

Doc2b
BM117900
1420667_at
0.606

Atm
AK021102
1428830_at
0.605

A930001A20Rik
BB280276
1431522_at
0.603

Pim1
AI323550
1435458_at
0.602

Susd4
BF455403
1460593_at
0.598


BB384728
1447012_at
0.598

4930538K18Rik
BB548441
1453632_at
0.596

Csf1r
AI323359
1419872_at
0.594

Fhad1
AW537651
1439190_at
0.59

MGI:1925112
BF719766
1438769_a_at
0.583

Cd276
NM_133983
1417599_at
0.578

Cpm
AK004327
1453009_at
0.574

1110059F07Rik
BE956260
1447533_at
0.573

Pts
NM_011220
1450660_at
0.567

Prss18
NM_011177
1448982_at
0.559

BC027092
BB445523
1435676_at
0.556

Cryab
NM_009964
1416455_a_at
0.536

H2-DMb1
NM_010387
1418638_at
0.534

Tmem25
BB245618
1436644_x_at
0.533

Dixdc1
BB758432
1435207_at
0.532

D630044F24Rik
AV285979
1454839_a_at
0.528

Sox10
BC018551
1451689_a_at
0.525

Dnaja4
BQ176119
1434196_at
0.518

Rfxdc2
BB148972
1460567_at
0.506

3110005L24Rik
AA611589
1431381_at
0.504

Lzp-s
AV066625
1436996_x_at
0.498

Islr
NM_012043
1418450_at
0.486

Pex2
AV327933
1451062_a_at
0.475

Lemd1
AV156411
1445642_at
0.472

Nrp2
BB752129
1435349_at
0.451

B230220N19Rik
BB539872
1442585_at
0.445

Fos
AV026617
1423100_at
0.42


BB047533
1446771_at
0.412


AI836671
1459747_at
0.394

Mcam
NM_023061
1416357_a_at
0.384

Rab27a
BB025283
1429123_at
0.326

4921517L17Rik
BC027204
1425047_a_at
0.31

Rps25
BM729504
1430978_at
0.308

Dixdc1
BC024834
1425256_a_at
0.289

C030014I23Rik
AK021081
1453044_at
0.287

Ppcdc
BB329391
1424336_at
0.244

Tipin
BB796715
1459720_x_at
0.199



Genes which are downregulated in Atm−/− relative to Atm+/+ in the Septin4 experiments. Microarray data from Atm+/+ and Atm−/− Septin4 IPs were compared in scatter plots, and filtered on expression values of at least 25 and selected for genes with Atm+/+ values at least 1.5 fold greater than Atm−/− values. Differential genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.




















































































































TABLE 28



Genes Upregulated in Atm−/− Pcp2 Data






Gene Symbol
GenBank Accession
Affymetrix ID
Fold Change








Nedd4
NM_010890
1421955_a_at
4.614

MGI:2136405
BB272589
1440386_at
4.155

AI451250
AI451250
1447436_at
4.04

Eva1
BC015076
1416236_a_at
3.992

Tex12
AF285582
1421183_at
3.409

C920006O11Rik
BB552195
1435704_at
2.684

AI593442
AV327193
1434052_at
2.584

Opcml
BB383296
1457446_at
2.538

Ddx6
BC021452
1424598_at
2.372

2900052N01Rik
AU067665
1436231_at
2.167

Cyp11a1
C87524
1439947_at
1.983

9330159M07Rik
BB214614
1441092_at
1.973


U58494
1451626_x_at
1.962

Btbd15
BC027138
1427271_at
1.948

Mod1
BC011081
1416632_at
1.891

Anln
BI690018
1433543_at
1.882

Nedd4
BG073415
1451109_a_at
1.869

E430024C06Rik
AU018141
1453238_s_at
1.853

AI593442
BF658761
1444026_at
1.834

Pml
BB667149
1456103_at
1.824

Pml
NM_008884
1448757_at
1.777

Ccrn4l
NM_010490
1448715_x_at
1.721

AI835735
AI835735
1459716_at
1.703

Pvrl3
NM_021495
1448673_at
1.698

C330006P03Rik
BB398124
1436387_at
1.681

5730601F06Rik
BI110339
1431206_at
1.671

Zfp191
BB579760
1426896_at
1.648

Rora
BB306272
1436326_at
1.644

Ccrn4l
AI987693
1455316_x_at
1.571

Zfp451
BI714076
1456350_at
1.556

Tpm1
AK002271
1423049_a_at
1.542

Peg3
AB003040
1417355_at
1.523

Chst2
BB770422
1442234_at
1.519

C230082I21Rik
AV317838
1455146_at
1.519

Ddx26
BB381966
1423275_at
1.509

Mjd
BQ176512
1434408_at
1.508

C79468
BQ086025
1459900_at
1.508

Mtf2
BG066919
1449115_at
1.507



Genes which are upregulated in Atm−/− relative to Atm+/+ in the Pcp2 experiments. Data from Atm+/+ and Atm−/− Pcp2 IPs were compared in scatter plots, and filtered on expression of at least 25 and with Atm−/− values at least 1.5 fold greater than Atm+/+ values. Enriched genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.

































































TABLE 29



Genes Downregulated in Atm−/− Pcp2 Data






Gene Symbol
GenBank Accession
Affymetrix ID
Fold Change








Nlgn3
BB308872
1456384_at
0.662

Ptbp1; LOC236294
BM195499
1458284_at
0.661

Fth1; Dpep2
AK002778
1427021_s_at
0.66

Zfyve21
BC019521
1424669_at
0.656

Gtf2f1
AV325174
1417699_at
0.655

1700027J05Rik
BB195788
1438638_x_at
0.653

MGI:1925112
BF719766
1438769_a_at
0.653

Tm2d1
AF353993
1451996_at
0.64


BB174864
1435126_at
0.64

Sh2d3c
AB043953
1415886_at
0.64

Ddx46
BG243238
1457744_at
0.639

Spa17
NM_011449
1417635_at
0.633

Boc
BB005556
1426869_at
0.626

4930549C01Rik
NM_026300
1421367_at
0.625


BQ175377
1443960_at
0.624

Adamts4
BB443585
1452595_at
0.62

Cryab
AV016515
1434369_a_at
0.619

BC027092
BB445523
1435676_at
0.617

D730049H07Rik
BQ173909
1424989_at
0.616

Fbf1
AF241249
1425979_a_at
0.615

AA589481
BE200208
1437276_at
0.615

Pts
NM_011220
1450660_at
0.614

Cyp4f13
NM_130882
1418767_at
0.613

BC020025
BC020025
1451298_at
0.607

AV340375
AV273541
1437737_at
0.604

Mmp17
NM_011846
1448598_at
0.596

Pcdh8
BB076893
1447825_x_at
0.588

Tcrb-V13
X14388
1427656_at
0.579

Cd4
U75219
1427779_a_at
0.575

Atm
AK021102
1428830_at
0.57

Decr2
BE952632
1423495_at
0.567

Tmod2
AK018223
1430153_at
0.566

Dusp7
BE136125
1452097_a_at
0.558

1200003M09Rik
BC011077
1423887_a_at
0.551

Dap
BC024876
1423790_at
0.546

2310004I03Rik
BM939621
1428698_at
0.543

Rps25
BM729504
1430978_at
0.513


BG066504
1435998_at
0.512

Rab3il1
AK004767
1428391_at
0.507

Tmem25
BB245618
1436644_x_at
0.481

C330003B14Rik
AU019881
1419958_at
0.469

Fmo2
NM_018881
1422904_at
0.436


BB393620
1443754_x_at
0.423

Dixdc1
BC024834
1425256_a_at
0.392

4930523C11Rik;
AV255799
1436129_at
0.387

Adam6

Bace1
AF200346
1421825_at
0.379

Lrrc49
BB484412
1447954_at
0.314


BC003855
1425469_a_at
0.313

2310030G06Rik
NM_025865
1449357_at
0.274

Tipin
BB796715
1459720_x_at
0.227

Ppcdc
BB329391
1424336_at
0.0711



Genes which are downregulated in Atm−/− relative to Atm+/+ in the Pcp2 experiments. Microarray data from Atm+/+ and Atm−/− Pcp2 IPs were compared in scatter plots, and filtered on expression values of at least 25 and selected for genes with Atm+/+ values at least 1.5 fold greater than Atm−/− values. Differential genes were selected based on one-way ANOVA analysis with a p-value of ≤0.05.










































































Identification of Functional Groups of Genes
To identify functional groups of genes which are enriched in the knockout cerebellum and each cell type, Gene Ontology (GO) analysis was performed using both DAVID (G. Dennis, Jr. et al., Genome Biol 4, P3 (2003)) and Genespring, each program essentially giving the same results. It was also essential to compare the gene lists and pertinent literature in order to completely analyze data as the GO database is incomplete. Genes enriched in total cerebellum of Atm−/− animals include those involved in cell cycle control (Tacc1, Cdkn1a (p21)) and apoptosis (Cdkn1a (p21)). Bergmann glial cells from Atm−/− animals are enriched in genes related to development and cellular differentiation, including BMP5. Purkinje cells from Atm−/− animals are enriched in genes involved in chromatin organization, nucleotide metabolism, and transcription regulation. These include transcription factors such as Tex12, Zfp191, Rora, Mtf2, and Mjd and genes involved in mRNA metabolism such as Ccrn41 and Ddx6. Several of these differentially regulated genes are either directly or indirectly associated with ataxia. Rora is an orphan retinoic acid receptor which is mutated in the staggerer mouse mutant (B. A. Hamilton et al., Nature 379, 736 (1996)). Mjd (Atxn3, Sca3) is the gene responsible for Machado-Joseph disease (Y. Kawaguchi et al., Nat Genet 8, 221 (1994)). Ddx6 is known to associate with Ataxin-2 in P-bodies, multiprotein complexes involved in mRNA degradation (U. Nonhoff et al., Mol Biol Cell 18, 1385 (2007); N. Cougot, S. Babajko, B. Seraphin, J Cell Biol 165, 31 (2004)). Ccrn41 is a deadenylase which is also found in P-bodies (N. Cougot, S. Babajko, B. Seraphin, J Cell Biol 165, 31 (2004)); deadenylation is a critical first step in the degradation of mRNA by P-bodies. Over-expression of these genes could potentially either enhance neurodegeneration of Purkinje cells, or protect against it.
Example 15
Screenint Methods for Parkinson's Disease
1) Translational Profiles of VTA and SNc Neurons
Dopamine producing (dopaminergic) neurons are present primarily in the ventral tegmental area (VTA) of the midbrain, substantia nigra pars compacta (SNc), and the arcuate nucleus of the hypothalamus Dopaminergic neurons of the SNc are particularly susceptible to neurodegeneration in Parkinson's disease (PD) and in pharmacologically-induced models of PD. The selective degeneration of the dopaminergic neurons in the substantia nigra pars compacta leads to PD but the exact cause for this nigral cell loss is still unknown. The ventral tegmental area dopaminergic neurons are relatively spared in comparison. The tremor, bradykinesia and rigidity seen in PD patients are in a large part caused by the loss of striatal dopamine innervation. Expression of a ribosomal protein (e.g. L10a) fused to a detectable tag, whose expression is regulated by SNc and VTA-specific gene regulatory regions can be used to explore the differential vulnerability of SNc cells and VTA cells in animal models of PD or subjects diagnosed with PD or Parkinsonian-like symptoms. By utilizing the BACarray and TRAP methods described herein, mice with molecularly tagged ribosomal proteins are produced. These are driven by regulatory sequences for cell-type specific expression in either the SNc or VTA ( FIG. 38 ). It is expected that differentially expressed mRNAs in SNc cells will be identified as potential therapeutic targets for the amelioration of PD.
2) Translational Profiles of VTA and SNc Neurons in PD
Dopaminergic cell loss mimicking what is seen in PD is induced with intracerebral injections of the neurotoxins 6-hydroxydopamine (6-OHDA) or 1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine (MPTP) either bilaterally or unilaterally in rodents (either mice or rats). By utilizing the BACarray and TRAP methods described herein, mice with molecularly tagged ribosomal proteins are produced. These are driven by regulatory sequences for cell-type specific expression in either the SNc or VTA ( FIG. 39 ). Three weeks following a 6-OHDA treatment (bilateral or unilateral treatment), gene translational profiles from the SNc and VTA neurons are obtained. These translational profiles are compared to reference profiles from mice treated with saline vehicle. It is expected that therapeutic targets relevant to PD will be obtained ( FIG. 40 ).
3) Screening for Therapeutic Targets in the Striatopallidal Cells for Antagonism or Stimulation
_High-frequency stimulation of the subthalamic nucleus (STN) is one neurosurgical procedure currently available for the alleviation of motor symptoms of PD and the debilitating medication-induced dyskinesias that often accompany the disease. Stimulation of the STN is achieved by implanting deep brain stimulation (DBS) electrodes precisely in the nucleus. The striatopallidal cells of the striatum tonically inhibit the Globus Pallidus External segment (GPe) and the Sub-thalamic nucleus (STN) ( FIG. 41 ). By inhibiting activity in striatopallidal cells with a small molecule or other non-electrical, non-surgery requiring method, it will be possible to alleviate the tonically inhibited STN neurons and alleviate PD symptoms, much in the way of deep brain stimulation. By utilizing the methods described herein and exemplified, using D2 BACarray mice one mRNA was found to be selectively enriched in striatopallidal neurons of the striatum is Gpr6 (Table 12). Gpr6 represents a target for which antagonists can be screened for and developed as a therapeutic for PD. In a similar manner, any mRNA found to be downregulated in striatopallidal cells represents a target for agonism as a therapeutic for PD.
Example 16
Screenint Methods for Obesity
The hypothalamus plays a central role in several regulatory and homeostatic processes including blood pressure, body temperature, sleep, fluid and electrolyte balance, and body weight. The lateral hypothalamus in particular is implicated as a feeding and satiety center. Food intake is regulated by hypothalamic neuropeptides which respond to peripheral signals. Homeostasis disrupted can lead to feeding and metabolic disorders leading to such diseases as obesity. Orexin, also named Hypocretin, is a excitatory neuropeptide hormone released by the lateral hypothalamus Orexin release modulates feeding behavior, wakefulness, and energy expenditure. A hypothalamic Hcrt (orexin) BACarray line has expression in the lateral hypothalamus
By utilizing the BACarray and TRAP methods described herein, mice with molecularly tagged ribosomal proteins are produced. These are driven by regulatory sequences for lateral hypothalamic cell-type specific expression under the control of regulatory sequences from the Hcrt (orexin) locus. By obtaining translational profiles from these animals, it is expected that targets relevant to obesity will be identified, in particular for feeding regulation.
While some embodiments of the present invention have been shown and described herein, it will be obvious to those skilled in the art that such embodiments are provided by way of example only. Numerous variations, changes, and substitutions will now occur to those skilled in the art without departing from the invention. It should be understood that various alternatives to the embodiments of the invention described herein may be employed in practicing the invention. It is intended that the following claims define the scope of the invention and that methods and structures within the scope of these claims and their equivalents be covered thereby.