(en)Provided herein are compositions and kits for single-stranded nucleic acid probes, and methods for making the single-stranded nucleic acid probes, where the single-stranded nucleic acid probes comprise a probe region having a predetermined sequence which is flanked by a 5′ region having a first restriction enzyme recognition sequence and flanked by a 3′ region having a second restriction enzyme recognition sequence, and a region which hybridizes to a capture nucleic acid molecule. The single-stranded nucleic acid probes are useful for solution-based capture methods.

1.ApplicationNumber: US-201013260906-A
1.PublishNumber: US-8986958-B2
2.Date Publish: 20150324
3.Inventor: RAYMOND CHRISTOPHER

4.Inventor Harmonized: RAYMOND CHRISTOPHER(US)

5.Country: US
6.Claims:
(en)Provided herein are compositions and kits for single-stranded nucleic acid probes, and methods for making the single-stranded nucleic acid probes, where the single-stranded nucleic acid probes comprise a probe region having a predetermined sequence which is flanked by a 5′ region having a first restriction enzyme recognition sequence and flanked by a 3′ region having a second restriction enzyme recognition sequence, and a region which hybridizes to a capture nucleic acid molecule. The single-stranded nucleic acid probes are useful for solution-based capture methods.

7.Description:
(en)This application claims the filing date benefit of U.S. Provisional Application No. 61/164,859, filed on Mar. 30, 2009. The contents of each foregoing patent applications are incorporated by reference in their entirety.
Throughout this application various publications are referenced. The disclosures of these publications in their entireties are hereby incorporated by reference into this application in order to more fully describe the state of the art to which this invention pertains.


FIELD
The present invention relates generally to the field of genomic analysis, and more particularly, to methods and kits for making libraries of single-stranded nucleic acid probes comprising predetermined sequences.
BACKGROUND
The ability to sequence deoxyribonucleic acid (DNA) accurately and rapidly is revolutionizing biology and medicine. The pharmacogenomics challenge is to comprehensively identify the genes and functional polymorphisms associated with the variability in drug response. Screens for numerous genetic markers performed for populations large enough to yield statistically significant data are needed before associations can be made between a given genotype and a particular disease.
The study of complex genomes, and in particular, the search for the genetic basis of disease in humans, requires genotyping on a massive scale, which is demanding in terms of cost, time, and labor. Such costly demands are even greater when the methodology employed involves serial analysis of individual DNA samples, i.e., separate reactions for individual samples. Resequencing of polymorphic areas in the genome that are linked to disease development will contribute greatly to the understanding of diseases, such as cancer, and therapeutic development. Oligonucleotide libraries are the cornerstone of sequence-based gene resequencing and digital profiling strategies. To realize the full commercial potential of various high-throughput sequencing platforms, the cost of generating oligonucleotide libraries must be reduced by a substantial amount. Thus, there is a need for cost-effective methods for preparing populations of high quality oligonucleotide probes with sufficient yield for use in high throughput sequencing platforms and solution based capture methods.
SUMMARY
Provided herein are methods for generating a population of single-stranded nucleic acid probes, each probe comprising a predetermined nucleotide sequence, the method comprising: (a) providing a starting population of linear double-stranded nucleic acid precursor molecules each precursor molecule having (i) a probe region having the predetermined sequence which is flanked at a 5′ and a 3′ end by a first and a second restriction enzyme recognition sequence for generating ligation substrates and for ligating a plurality of the double-stranded nucleic acid precursor molecules into head-to-tail concatemers (ii) the 5′ flanking region including the first restriction enzyme recognition sequence and (iii) the 3′ flanking region including the second restriction enzyme recognition sequence; (b) contacting the 5′ and 3′ flanking regions of the linear double-stranded nucleic acid precursor molecules with the first and second restriction enzymes to cleave the first and second restriction enzyme recognition sequences so as to generate the ligation substrates; (c) ligating the ligation substrates together so as to generate a plurality of random head-to-tail concatemers; (d) amplifying the plurality of head-to-tail concatemers; (e) contacting the amplified head-to-tail concatemers with the first and second restriction enzymes so as to release a plurality double-stranded monomer linear precursor molecules; and (f) selectively removing one strand of the double-stranded monomer linear precursor molecules so as to generate a population of single-stranded nucleic acid probes, each probe comprising the predetermined nucleotide sequence.
In one embodiment, the single-stranded nucleic acid probes further comprise a region which hybridizes to a capture nucleic acid molecule.
In yet another embodiment, the selectively removing one strand from the double-stranded monomer linear precursor molecules comprises: (a) contacting the released precursor molecules of step (e) above with alkaline phosphatase; (b) contacting the released precursor molecules of step (e) above with a third restriction enzyme which cleaves the third restriction enzyme recognition sequence; and (c) contacting the released precursor molecules of step (e) above with an exonuclease so as to selectively degrade the one strand of the double-stranded monomer linear precursor molecules.
In one embodiment, the exonuclease is lambda exonuclease.
In another embodiment, the members of the starting population of the linear double-stranded nucleic acid precursor molecules each comprise the same nucleotide sequence in the 5′ flanking region or each comprise the same nucleotide sequence in the 3′ flanking region.
In another embodiment, the 3′ flanking region further comprises a third restriction enzyme recognition sequence.
In another embodiment, the members of the starting population of the linear double-stranded nucleic acid precursor molecules each comprise the same predetermined sequences or different predetermined sequences.
In another embodiment, the ligation substrates of step (b) comprise overhanging nucleic acid ends capable of annealing together.
In another embodiment, the first or second restriction enzyme recognition sequence is cleaved by a type II restriction enzyme.
In another embodiment, the first or second restriction enzyme recognition sequence is cleaved by a Bsm1 enzyme.
In another embodiment, each predetermined nucleotide sequence in the population of linear double-stranded nucleic acid precursor molecules comprise a nucleotide sequence which is at least 95% identical to at least a portion of a sense or anti-sense strand of a target nucleic acid sequence.
In another embodiment, the predetermined sequence hybridizes to one target sequence or hybridizes to different target sequences.
In another embodiment, the predetermined sequences in the population of linear double-stranded nucleic acid precursor molecules hybridize to at least 10 different exon nucleotide sequences.
In another embodiment, the predetermined sequences in the population of linear double-stranded nucleic acid precursor molecules hybridize to at least 1000 different exon nucleotide sequences.
In another embodiment, the predetermined sequences hybridize to the target sequence at an interval of at least every 35 bases across the target sequence.
In another embodiment, the predetermined sequences hybridize to the target sequence of interest at an interval of one base across the target sequence.
In another embodiment, the probe region comprises 20-200 nucleotides.
In another embodiment, the predetermined nucleotide sequence comprises 10-50 nucleotides.
In another embodiment, the region of the single-stranded nucleic acid probe which hybridizes to the capture nucleic acid molecule comprises 10-50 nucleotides.
In another embodiment, the amplifying according to step (d) comprises isothermal amplification.
In another embodiment, the amplifying according to step (d) comprises random amplification primers.
In another embodiment, the random amplification primers each comprise a random 7-mer oligonucleotide and two additional nitroindole residues at the 5′ end.
In another embodiment, the random amplification primers each comprise a random 7-mer oligonucleotide and a phosphorothioate linkage to the 3′ end.
In another embodiment, the capture nucleic acid molecule further comprises a protein binding partner.
In another embodiment, the protein binding partner is biotin.
In another embodiment, each single-stranded nucleic acid probe comprises (i) the predetermined nucleotide sequence having a nucleotide sequence which is at least 95% identical to at least a portion of a sense or an anti-sense strand of a target nucleic acid sequence and (ii) a region which hybridizes to a capture nucleic acid molecule.
Provided herein are also a population of single-stranded nucleic acid probes generated by the disclosed methods.
Provided herein are also methods, wherein the starting population of linear double-stranded nucleic acid precursor molecules is generated by steps comprising: (a) providing a population of a first single-stranded nucleic acid molecule comprising the 5′ flanking region, the probe region which comprises the predetermined sequence, and the capture sequence; (b) providing a population of a second single-stranded nucleic acid molecules comprising the sequence which is complementary to the capture sequence, and the 3′ flanking region; (c) annealing the first and second populations of the single-stranded nucleic acid molecules to form a nucleic acid duplex having overhanging 5′ ends; and (d) conducting a polymerase-dependent strand extension reaction on the overhanging 5′ ends so as to generate the population of double-stranded nucleic acid precursor molecules.
Provided herein are also, methods for enriching a target nucleic acid sequence of interest from a nucleic acid library, comprising: (a) contacting the population of single-stranded nucleic acid probes of the method above with the nucleic acid library having at least one target nucleic acid sequence of interest to form a mixture having unhybridized nucleic acid sequences and duplexes, each duplex having the single-stranded nucleic acid probe hybridized to the target nucleic acid sequence of interest; (b) contacting the duplexes with a population of capture nucleic acid molecules to form complexes having the single-stranded nucleic acid probe hybridized to the target nucleic acid sequence of interest and hybridized to the capture nucleic acid molecule; (c) separating the complex from the mixture; and (d) eluting the target nucleic acid sequence of interest from the complex.



DESCRIPTION OF THE DRAWINGS
FIG. 1A illustrates a population of target capture probes ( 200 ) for solution-based capture, designed to provide low density coverage of a target exon ( 10 ), as described in Example 1;
FIG. 1B illustrates a population of target capture probes ( 200 ) for solution-based capture, designed to provide high density coverage of a target exon ( 10 ), as described in Example 1;
FIG. 1C is a schematic diagram of a representative target capture probe ( 200 ) comprising a target-specific binding region ( 202 ) bound to a nucleic acid target ( 10 ), and a region for binding to a capture reagent ( 204 ) bound to a universal adaptor oligonucleotide ( 300 ) comprising a moiety ( 310 ) that binds to a capture reagent;
FIG. 2 illustrates a method of enriching a population of DNA molecules for target regions of interest using capture probes ( 200 ) generated in accordance with an embodiment of the present invention;
FIG. 3 is a flow chart of the steps of a method for generating a population of capture probes in accordance with various embodiments of the present invention;
FIG. 4 (SEQ ID NOS: 69 and 70) illustrates a method of generating a population of single-stranded capture probes ( 200 ) in accordance with an embodiment of the present invention;
FIG. 5 illustrates more detail of the method illustrated in FIG. 4 ; and
FIG. 6 graphically illustrates the fold enrichment obtained using capture probes generated in accordance with the methods of the present invention, as described in Example 2.



DETAILED DESCRIPTION
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art to which these inventions belong. All patents, patent applications, published applications, treatises and other publications referred to herein, both supra and infra, are incorporated by reference in their entirety. If a definition and/or description is explicitly or implicitly set forth herein that is contrary to or otherwise inconsistent with any definition set forth in the patents, patent applications, published applications, and other publications that are herein incorporated by reference, the definition and/or description set forth herein prevails over the definition that is incorporated by reference.
The practice of the present disclosure will employ, unless otherwise indicated, conventional techniques of molecular biology, microbiology and recombinant DNA techniques, which are within the skill of the art. Such techniques are explained fully in the literature. See, for example, Sambrook, J., and Russell, D. W., 2001, Molecular Cloning: A Laboratory Manual, Third Edition; Ausubel, F. M., et al., eds., 2002, Short Protocols in Molecular Biology, Fifth Edition.
As used herein, the terms “comprising” (and any form or variant of comprising, such as “comprise” and “comprises”), “having” (and any form or variant of having, such as “have” and “has”), “including” (and any form or variant of including, such as “includes” and “include”), or “containing” (and any form or variant of containing, such as “contains” and “contain”), are inclusive or open-ended and do not exclude additional, unrecited additives, components, integers, elements or method steps.
As used herein, the terms “a,” “an,” and “the” and similar referents used herein are to be construed to cover both the singular and the plural unless their usage in context indicates otherwise. Accordingly, the use of the word “a” or “an” when used in the claims or specification, including when used in conjunction with the term “comprising”, may mean “one,” but it is also consistent with the meaning of “one or more,” “at least one,” and “one or more than one.”
As used herein, the term “nucleic acid molecule” and its variants encompasses both deoxyribonucleotides and ribonucleotides and refers to a polymeric form of nucleotides including two or more nucleotide monomers. The nucleotides can be naturally occurring, artificial, and/or modified nucleotides.
As used herein, an “isolated nucleic acid” and its variants is a nucleic acid molecule that exists in a physical form that is non-identical to any nucleic acid molecule of identical sequence as found in nature; “isolated” does not require, although it does not prohibit, that the nucleic acid so described has itself been physically removed from its native environment. For example, a nucleic acid can be said to be “isolated” when it includes nucleotides and/or internucleoside bonds not found in nature. When, instead, composed of natural nucleosides in phosphodiester linkage, a nucleic acid can be said to be “isolated” when it exists at a purity not found in nature, where purity can be adjudged with respect to the presence of nucleic acids of other sequences, with respect to the presence of proteins, with respect to the presence of lipids, or with respect to the presence of any other component of a biological cell, or when the nucleic acid lacks a sequence that flanks an otherwise identical sequence in an organism's genome, or when the nucleic acid possesses a sequence not identically present in nature. As so defined, “isolated nucleic acid” includes nucleic acids integrated into a host cell chromosome at a heterologous site, recombinant fusions of a native fragment to a heterologous sequence, recombinant vectors present as episomes, or as integrated into a host cell chromosome.
As used herein, “subject” and its variants refers to an organism or to a cell sample, tissue sample, or organ sample derived therefrom, including, for example, cultured cell lines, biopsy, blood sample, or fluid sample containing a cell. For example, an organism may be an animal, including but not limited to, an animal such as a cow, a pig, a mouse, a rat, a chicken, a cat, a dog, etc., and is usually a mammal, such as a human.
As used herein, the term “specifically bind” and its variants refers to two components (e.g., target-specific binding region and target) that are bound (e.g., hybridized, annealed, complexed) to one another sufficiently that the intended capture and enrichment steps can be conducted. As used herein, the term “specific” refers to the selective binding of two components (e.g., target-specific binding region and target) and not generally to other components unintended for binding to the subject components.
As used herein, the term “high stringency hybridization conditions” and its variants means any condition in which hybridization will occur when there is at least 95%, preferably about 97% to 100% nucleotide complementarity (identity) between the nucleic acid sequences of the nucleic acid molecule and its binding partner. However, depending upon the desired purpose, the hybridization conditions may be “medium stringency hybridization,” which can be selected that require less complementarity, such as from about 50% to about 90% (e.g., 60%, 70%, 80%, 85%). The comparison of sequences and determination of percent identity between two sequences can be accomplished using a mathematical algorithm of Karlin and Altschul (Proc. Natl. Acad. Sci. USA 87:2264-2268 (1990)), modified as in Karlin and Altschul (Proc. Natl. Acad. Sci. USA 90:5873-5877 (1993)). Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al. (J. Mol. Biol. 215:403-410 (1990)).
As used herein, the term “complementary” and its variants refers to nucleic acid sequences that are capable of base-pairing according to the standard Watson-Crick complementary rules. That is, the larger purines will base pair with the smaller pyrimidines to form combinations of guanine paired with cytosine (G:C) and adenine paired with either thymine (A:T) in the case of DNA, or adenine paired with uracil (A:U) in the case of RNA.
As used herein, the term “target” and its variants refers to a nucleic acid molecule or polynucleotide whose presence and/or amount and/or sequence is desired to be determined and which has an affinity for a given target capture probe. Examples of targets include regions of genomic DNA, PCR amplified products derived from RNA or DNA, DNA derived from RNA or DNA, ESTs, cDNA, and mutations, variants or modifications thereof.
As used herein, the term “predetermined nucleic acid sequence” and its variants means that the nucleic acid sequence of a nucleic acid probe is known and was chosen before synthesis of the nucleic acid molecule in accordance with the invention disclosed herein.
As used herein, the term “essentially identical” and its variants as applied to synthesized and/or amplified nucleic acid molecules refers to nucleic acid molecules that are designed to have identical nucleic acid sequences, but that may occasionally contain minor sequence variations in comparison to a desired sequence due to base changes introduced during the nucleic acid molecule synthesis process, amplification process, or due to other processes in the method. As used herein, essentially identical nucleic acid molecules are at least 95% identical to the desired sequence, such as at least 96%, such as at least 97%, such as at least 98%, such as at least 99% identical, or absolutely identical, to the desired sequence.
As used herein, the term “resequencing” and its variants refers to a technique that determines the sequence of a genome of an organism using a reference sequence that has already been determined. It should be understood that resequencing may be performed on both the entire genome/transcriptome of an organism or a portion of the genome/transcriptome large enough to include the genetic change of the organism as a result of selection. Resequencing may be carried out using various sequencing methods, such as any sequencing platform amenable to producing DNA sequencing reads that can be aligned back to a reference genome, and is typically based on highly parallel technologies such as, for example, dideoxy “Sanger” sequencing, pyrosequencing on beads (e.g., as described in U.S. Pat. No. 7,211,390, assigned to 454 Life Sciences Corporation, Branford, Conn.), ligation based sequencing on beads (e.g., Applied Biosystems Inc,/Invitrogen), sequencing on glass slides (e.g., Illumina Genome Analyzer System, based on technology described in WO 98/44151 (Mayer, P., and Farinelli, L.), microarrays, or fluorescently labeled micro-beads.
As used herein, the term “target nucleotide” and its variants refers to a nucleic acid molecule or polynucleotide in a starting population of nucleic acid molecules having a target sequence whose presence and/or amount and/or nucleotide sequence is desired to be determined and which has an affinity for a given target capture probe.
As used herein, the term “target sequence” and its variants refers generally to a nucleic acid sequence on a single strand of nucleic acid. The target sequence may be a portion of a gene, a regulatory sequence, genomic DNA, cDNA, RNA including mRNA and rRNA, or others. The target sequence may be a target sequence from a sample, or a secondary target such as a product of an amplification reaction.
As used herein, the term “processing” and its variants refers generally to a manipulation of a precursor nucleic acid substrate into a processed form of the substrate, such as by cleavage with a restriction endonuclease, modification and/or amplification with DNA polymerases, manipulation of DNA termini (e.g., by adding terminal 5′ phosphates with a polynucleotide kinase or removing 5′ terminal phosphates with a suitable phosphatase), degradation of unwanted DNA strands with exonuclease, and the like.
As used herein, the term “head to tail concatemer” and its variants refers to at least two or more monomeric structures each having a first end and a second end, such as double-stranded nucleic acid molecules, covalently joined in the configuration of the second end of the first monomer joined to the first end of the second monomer.
Other objects, features and advantages of the disclosed compositions, methods, systems and kits will become apparent from the following detailed description. It should be understood, however, that the detailed description and the specific examples, while indicating specific embodiments, are given by way of illustration only, since various changes and modifications within the spirit and scope of the inventions provided herein will become apparent to those skilled in the art from this detailed description.
Provided herein are compositions, systems, methods and kits for generating a population of single-stranded nucleic acid probes, each probe comprising a predetermined nucleic acid sequence. The methods of the invention are useful in any situation in which it is desirable to make populations of single-stranded nucleic acid molecules (hundreds to thousands, to tens of thousands, to hundreds of thousands, to millions of oligonucleotides), wherein each nucleic acid molecule has a predetermined nucleic acid sequence. For example, the methods may be used to generate a high quantity of a complex population (e.g., library) of single-stranded nucleic acid probes, while maintaining a uniform representation of the individual nucleic acid probes within the population. The applications for pools of oligonucleotides include, but are not limited to, using the oligonucleotides to generate primers for PCR amplification, primers for multiplexing PCR and transcription, probes for SNP (single nucleotide polymorphism) detection, and libraries of nucleic acid probes for genomic analysis, RNA expression analysis, including siRNA and shRNA expression analysis. In some embodiments, the methods according to this aspect of the invention are used to generate a library of target specific probes for solution based capture methods, as described in Examples 1-3 herein.
The methods according to this aspect of the invention comprise (a) providing a starting population of double-stranded nucleic acid precursor molecules, wherein each precursor molecule in the starting population comprises a probe region comprising a predetermined sequence that is flanked on the 5′ end by a 5′ flanking region comprising a first processing site and is flanked on the 3′ end by a 3′ flanking region comprising a second processing site, wherein the first and second processing sites are selected to generate ligation substrates for ligation of a plurality of the double-stranded nucleic acid precursor molecules into head-to-tail concatemers; (b) processing the 5′ and 3′ flanking regions of the double-stranded nucleic acid precursor molecules to generate ligation substrates; (c) ligating the ligation substrates together to generate head-to-tail concatemers; (d) amplifying the head-to-tail concatemers; (e) processing the amplified head-to-tail concatemers to release double-stranded monomer precursor molecules; and (f) selectively removing the complement strand of the double-stranded monomer precursor molecules to generate a population of single-stranded nucleic acid probes each probe a predetermined nucleic acid sequence.
In one embodiment of the method, a population of target specific capture probes (e.g., a library of capture probes) is generated that may be used in solution based capture methods for enriching a population of DNA molecules for one or more target sequences of interest, such as for resequencing analysis. In accordance with this embodiment, each single-stranded capture probe oligonucleotide in the population of capture probes comprises (i) a target-specific binding region consisting of a nucleic acid sequence that is at least 95% identical to at least a portion of the sense or antisense strand of a target nucleic acid sequence of interest, and (ii) a region for binding to a capture reagent. The methods according to this embodiment of the invention can be used to create populations of single-stranded capture nucleic acid molecules (i.e., capture probes). A population of capture probes is also referred to as a “library” of capture probes. The capture probes generated using the methods described herein may be used for solution based capture to enrich for targets of interest.
FIG. 1C illustrates a representative capture probe 200 comprising a target-specific binding region 202 and a region 204 for binding to a capture reagent 300 . In the embodiment shown in FIG. 1C , the capture reagent 300 is a universal adaptor oligonucleotide comprising a moiety 310 that binds to a capture reagent. The capture probes 200 generated using the methods of the present invention may be used to enrich a library for target nucleic acid regions of interest in a method referred to as solution based capture.
A representative method of solution based capture is illustrated in FIG. 2 . The capture probes 200 (the capture probe 200 is representative of a population of capture probes) illustrated in FIG. 2 comprise a target-sequence specific binding region 202 and a capture reagent binding region 204 that hybridizes to a universal adaptor oligonucleotide 300 comprising a moiety 310 that binds to a capture reagent 400 . As shown in FIG. 2 at step A, in one embodiment, solution based capture comprises contacting a library of DNA molecules 50 comprising a subpopulation of nucleic acid target insert sequences of interest 10 flanked by a first primer binding region 22 and a second primer binding region 32 . The plurality of nucleic acid insert regions in the library include one or more target sequences 10 , and can include enough different nucleic acid sequences to cover (i.e., represent) part or all of a source nucleic acid including, without limitation, the genome of an organism, a genomic locus, a cDNA library, a whole transcriptome of an organism, the exome of an organism and the like. As used herein the term “exome” refers to protein coding regions, promoters, known ncRNAs (non-coding RNAs) and UTRs, altogether comprising about 2% of the human genome.
As shown in FIG. 2 at step B, the target-specific binding region 202 of the target capture probe 200 binds to a substantially complementary target nucleic acid sequence 10 , shown as an insert region 10 of a nucleic acid molecule 50 in a library of nucleic acid molecules. The universal adaptor oligonucleotide 300 is present at an equal concentration as the capture probes 200 , and hybridizes to the capture reagent binding region 204 . The moiety 310 (e.g., biotin) attached to the universal oligo adaptor 300 is then contacted with a capture reagent 400 (e.g., a magnetic bead) having a binding region 410 (e.g., streptavidin coating) and the complex is pulled out of solution with a sorting device 500 (e.g., a magnet) that binds to the capture reagent 400 .
Any library of DNA molecules comprising a subpopulation of nucleic acid target insert sequences of interest may be enriched using the solution based capture methods described herein. In some embodiments, the library of DNA molecules comprises a plurality of distinct insert sequences flanked by a first primer binding region and a second primer binding region within a larger population of nucleic acid insert sequences flanked by the first primer binding region and the second primer binding region may be enriched for target sequences using the capture probes 200 generated using the methods disclosed herein. For example, a library of DNA molecules comprising a subpopulation of nucleic acid target insert sequences of interest flanked by a first primer binding region and a second primer binding region within a larger population of nucleic acid insert sequences flanked by the first primer binding region and the second primer binding region may be enriched using the capture probes generated using the methods of the invention. In some embodiments, the library of DNA molecules further comprises at least one anchor probe binding site, such as a flow cell binding site for binding to a flow cell sequencing platform, such as an Illumina Genome Analyzer for sequence analysis.
The use of solution-based capture to enrich a library allows for the efficient creation of resequencing samples (sequence-ready libraries) that are largely composed of target sequences, as demonstrated in Example 2.
The Design of the Target Capture Probe 200
The general design of the target capture probe 200 is described as follows. As shown in FIG. 1C , the target capture probe 200 comprises a target sequence-specific binding region 202 and a capture reagent region 204 for binding to a capture reagent 300 .
The length of a target capture probe 200 is typically in the range of from 20 nucleotides to about 200 nucleotides, such as from about 20 nucleotides to about 150 nucleotides, such as from about 30 nucleotides to about 100 nucleotides, or such as from about 40 nucleotides to about 80 nucleotides.
The target-specific binding region 202 of the target capture probe 200 is typically from about 10 to about 150 nucleotides in length (e.g., 35 nucleotides, 50 nucleotides, 100 nucleotides) and is chosen to specifically hybridize to a target sequence of interest. In one embodiment, the target capture probe is about 60 to 80 nucleotides in length, comprising a target-specific binding region of about 20 to 40 nucleotides in length, such as about 35 nucleotides in length.
The target specific binding region 202 comprises a sequence that is substantially complementary (i.e., at least 95% identical, at least 96% identical, at least 97% identical, at least 98% identical, at least 99% identical, or 100% identical) to the target sequence of interest. Described in another way, for a target specific binding region 202 having a sequence with a length of from 10 to 100 nucleotides that is at least 95% complementary or at least 95% identical to a target sequence of interest, a region 202 that is from 20 nt to 35 nt in length may contain 1 mismatch, a region 202 that is from 40 nt to 50 nt in length may contain up to 2 mismatches, a region 202 that is from 60 nt to 70 nt may contain up to 3 mismatches, a region 202 that is from 80 nt to 90 nt may contain up to 4 mismatches, and a region 202 having a length of 100 nt may contain up to 5 mismatches with the target sequence.
In one embodiment, the method is used to generate a plurality of capture probes 200 each comprise a target-specific binding region 202 consisting of a sequence with a length of 35 nucleotides, that is at least 95% complementary, or at least 95% identical to a target sequence of interest (i.e., up to 1 mismatch with the target sequence).
The capture oligonucleotides may be designed to bind to a target region at selected positions spaced across the target region at various intervals. The capture oligo design and target selection process may also take into account genomic features of the target region such as genetic variation, G:C content, predicted oligo Tm, and the like. One of skill in the art can use art-recognized methods to determine the features of a target binding region that will hybridize to the target with minimal non-specific hybridization. For example, one of skill can determine experimentally the features such as length, base composition, and degree of complementarity that will enable a nucleic acid molecule (e.g., the target-specific binding region of a target capture probe) to specifically hybridize to another nucleic acid molecule (e.g., the nucleic acid target) under conditions of selected stringency, while minimizing non-specific hybridization to other substances or molecules. For example, for an exon target of interest, a target gene sequence is retrieved from a public database such as GenBank, and the sequence is searched for stretches of from 25 to 150 bp with a complementary sequence having a GC content in the range of 45% to 55%. The identified sequence may also be scanned to ensure the absence of potential secondary structure and may also be searched against a public database (e.g., a BLAST search) to ensure a lack of complementarity to other genes, as described in Example 3.
In some embodiments of the method, a set of capture probes (e.g., a library) is designed to specifically bind to target sequences across a genomic location, such as across a chromosomal region, and the capture probes are contacted with nucleic acid molecules from a total genomic library, or a whole-transcriptome library in order to analyze the whole transcriptome across the chosen genomic locus. In some embodiments of the method, a set of capture probes is designed to specifically bind to a plurality of target regions, such as the exons of a single gene, or multiple genes, such as at least 5 genes, at least 10 genes, at least 20 genes, at least 50 genes, at least 75 genes, at least 200 genes, at least 1000 genes, at least 10,000 genes, or more, as described in Examples 1-3. For example, as demonstrated in Example 3 herein, the methods according to this aspect of the invention were used to generate a set of capture probes comprising 1,148,286 distinct target-specific 35mer regions that were designed to capture all the exons from a total of 25,341 annotated genes from a sample containing nucleic acid sequences derived from a human.
In some embodiments of the method, a set of capture probes is designed to specifically bind to a genomic locus known to be associated with a clinical outcome or disease, or disease risk.
In some embodiments, the methods of the invention are used to capture and sequence a modified or mutated target, such as to determine the presence of a particular single nucleotide polymorphism (SNP), or deletion, addition, or other modification. In accordance with such embodiments, the set of target capture probes are typically designed such that there is a very dense array of capture probes that are closely spaced together such that a single target sequence, which may contain a mutation, will be bound by multiple capture probes that overlap the target sequence. For example, capture probes may be designed that cover every base of a target region, on one or both strands, (e.g., head to tail), or that are spaced at intervals of every 2, 3, 4, 5, 10, 15, 20, 40, 50, 90, 100, or more bases across a sequence region.
As another example, the selection of the target capture probes over a target region of interest is based on the size of the target region. For example, for a target region of less than 100 nucleotides in length, capture probes (either sense, antisense, or both) are typically designed to hybridize to target sequences spaced apart by from 0 to 100 nucleotides, such as every 45 nucleotides, or every 35 nucleotides. As another example, for a target region greater than 200 nucleotides, capture probes (either sense, antisense, or both) are typically designed to hybridize to target sequences spaced apart by from 0 to 200 nucleotides, such as at 45 to 65 nucleotide intervals, or at higher density coverage such as every 35 nucleotide intervals. In one embodiment, for a target region greater than 200 nucleotides (e.g., a 200,000-nucleotide target region), a set of sense and antisense capture probes are designed that are each about 35 nucleotides in length and are spaced about 45 nucleotides apart across the target region (alternating sense/antisense) in order to saturate the region (e.g., “tile” across the region of interest).
In one embodiment, a library of target specific probes are designed to bind to a desired target with high density coverage, such that at least one capture probe binds to at least every 35 nucleotide region of the target sequence. In one embodiment, a library of target specific probes are designed to bind to every nucleotide of the target, with alternating binding regions on the sense and antisense strands of the target sequence.
Referring now to FIG. 1C , in one embodiment, the target capture probes 200 each comprise a capture reagent binding region 204 that hybridizes to a universal adaptor oligonucleotide 300 comprising a moiety 310 that binds to a capture reagent 400 . The capture reagent binding region 204 of the target capture probe 200 is typically from about 10 to about 50 nucleotides in length (e.g., 10 nucleotides, 15 nucleotides, 20 nucleotides, 35 nucleotides) and is chosen to specifically hybridize to a universal adaptor oligonucleotide comprising a moiety 310 that binds to a capture reagent. In one embodiment, the target capture probe is about 60 to 80 nucleotides in length, comprising a capture reagent binding region of about 10 to 40 nucleotides in length, such as about 35 nucleotides in length.
In operation, as shown in FIG. 2 , the target-specific binding region 202 of the target capture probes bind to a complementary or substantially complementary nucleic acid sequence contained in a nucleic acid target 10 (i.e., an insert of a nucleic acid molecule in a library, or a genomic region of nucleic acids isolated from a sample). A universal adaptor oligonucleotide 300 is present at an equal concentration as the capture probes 200 , and hybridizes to the capture reagent binding region 204 . The moiety 310 (e.g., biotin) attached to the universal adaptor oligonucleotide 300 is then contacted with a capture reagent 400 (e.g., a magnetic bead) having a binding region 410 (e.g., streptavidin coating) and the complex is pulled out of solution with a sorting device 500 (e.g., a magnet) that binds to the capture reagent 400 .
As shown in FIG. 3 , the method 600 of generating a population of target-specific capture probes in accordance with one embodiment of the invention, includes the step 610 of providing a starting population of double-stranded capture probe precursors, each precursor comprising a capture probe region 200 (comprising a target-sequence specific binding region 202 and a capture reagent binding region 204 ), flanked by a 5′ flanking region 210 and a 3′ flanking region 220 . The 5′ flanking region 210 comprises a first processing site and the 3′ flanking region 220 comprises a second processing site, wherein the first and second processing sites are selected to generate ligation substrates capable of ligation into head-to-tail concatemers. An exemplary double-stranded capture probe precursor 230 is illustrated in FIG. 4 at step A. As shown in FIG. 4 at step A, the exemplary double-stranded capture probe precursor 230 comprises a capture probe region 200 flanked on the 5′ end by flanking region 210 comprising a first processing site (e.g., a first Bsm1 site) and is flanked on the 3′ end by flanking region 220 comprising a second processing site (e.g., a second Bsm1 site). In some embodiments, the 3′ flanking region 220 further comprises a third processing site (e.g., a Psi1 site or a HindIII site) selected to precisely cleave off the 3′ flanking region.
The 5′ flanking region 210 is typically from about 4 to about 30 nucleotides in length, such as from about 5 to about 15 nucleotides in length, or from about 5 to 10 nucleotides in length. The nucleotide sequence of the 5′ flanking region 210 is chosen to provide a first processing site, such as a first restriction enzyme recognition site, such as a type II restriction endonuclease (e.g., Bsm1), to generate the desired nucleotide overhang at the first end of the double-stranded precursor molecule for ligation in a head to tail configuration, and for precise cleavage of the 5′ flanking region 210 from the capture probe 200 region.
The 3′ flanking region 220 is typically from about 4 to about 30 nucleotides in length, such as from about 5 to about 25 nucleotides in length, or from about 10 to 20 nucleotides in length. The nucleotide sequence of the 3′ flanking region 220 is chosen to provide a second processing site, such as a second restriction enzyme recognition site (e.g., Bsm1), to generate the desired nucleotide overhang at the second end of the double-stranded molecule for ligation. In some embodiments, the 3′ flanking region 220 further comprises an additional processing site (e.g., a third restriction enzyme recognition site, such as Psi1 or HindIII) for precise cleavage of the 3′ flanking region 220 from the capture probe 200 region.
As illustrated in FIG. 4 , step B, the population of double-stranded capture probe precursors 230 are processed into ligation substrates that form head-to-tail concatemers upon ligation with one another. Accordingly, the first processing site in the 5′ flanking region and the second processing site in the 3′ region are chosen such that upon digestion of the first and second processing sites in the double-stranded precursor 230 , nucleotide overhangs are left on each end of the double-stranded precursor that can only ligate with one another in a head to tail configuration (e.g., a “CC” overhang on the first end of the molecule and a “GG” overhang on the second end of the molecule, as illustrated in FIG. 4 , step C).
It will be understood by one of skill in the art that Bsm1 is a non-limiting example of a type II restriction endonuclease that recognizes an asymmetric sequence and cleaves outside of that asymmetric sequence to yield the desired nucleotide overhangs at the first end and the second end of the double-stranded precursor molecule for ligation in a head to tail configuration. For example, dozens of type II restriction endonuclease enzymes are commercially available and known to those of skill in the art (see, e.g., New England Biolabs catalogue and REBASE web site) from which one of skill in the art could design a first processing site in the 5′ flanking region and a second processing site in the 3′ flanking region to provide ligation substrates that could only ligate in a head to tail configuration.
The double-stranded capture probe precursor 230 for use in various embodiments of the methods of the invention may be generated using a variety of methods. In one embodiment, the double-stranded capture probe precursor 230 is generated by synthesizing pairs of single-stranded complementary sense and antisense oligonucleotides comprising the full length sequence of the capture probe precursor 230 and annealing the strands together to form the double-stranded capture probe precursor. In another embodiment, a ligation ready, double-stranded capture probe precursor 230 having from one to four nucleotide overhangs on each end, is generated by annealing a pair of synthesized complementary strands together to produce the desired nucleotide overhang that will result in head-to-tail concatemers upon ligation.
In another embodiment, as shown in FIG. 4 , step A, and demonstrated in Example 1, a population of double-stranded precursor molecules 230 is generated by first synthesizing a population of single-stranded oligonucleotide precursors 100 comprising a 5′ flanking region 210 , a target-specific binding region 202 , and a portion of the capture reagent binding region 204 (e.g., at least 10 to 20 nucleotides) and annealing the synthesized population of oligos 100 with a common reverse primer oligonucleotide 150 to form the double-stranded capture probe precursor molecules 230 . As illustrated in FIG. 4 , the common reverse primer oligo 150 is designed to hybridize to the region 204 for binding to a capture reagent on the synthesized oligos 100 , and includes the complement of the remaining portion of the region 204 , as well as the complement of the 3′ flanking region 220 . An advantage to the second strand fill in reaction with the reverse primer oligo 150 is the ability to change the 3′ flanking region through the use of a reverse primer oligo 150 with different sequences in order to incorporate different restriction enzyme sites or other desired sequences in the 3′ flanking region 220 of the double-stranded capture probe precursor molecule 230 . For example, other sequences that could be included in the 3′ flanking region 220 include primer binding sites, protein binding sites (e.g., for binding a prokaryotic polymerase such as T7 RNA polymerase), modified nucleotides for purification (e.g., biotinylated residues), or methylated 5-methylated 5-methyl-cytosine residues for resistance to bisulphate conversion.
Referring again to FIG. 3 , at step 620 , the population of double-stranded capture probe precursors 230 are processed into ligation substrates that form head-to-tail concatemers upon ligation with one another, as illustrated in FIG. 4 , step B. One of skill in the art can use art-recognized methods to determine the sequence of a 5′ flanking region 210 and a 3′ flanking region 220 that will provide suitable processing sites, such as restriction endonuclease recognition sites, for generating a first overhang of from 1 to 12 nucleotides on the first end and a second overhang of from 1 to 12 nucleotides on the second end of the double-stranded capture probe precursor 230 . In a non-limiting example, as described in Example 1, the double-stranded capture probe precursors 230 are designed to include a 5′ flanking region 210 comprising a first restriction enzyme site for Bsm1, in order to create a two nucleotide “GG” overhang at the first end of the precursor 230 molecule, and a 3′ flanking region 220 comprising a second restriction enzyme site for Bsm1, in order to create a two nucleotide “CC” overhang at the second end of the precursor 230 after digestion with Bsm1 to facilitate ligation into head-to-tail concatemers, as illustrated in FIG. 4 , step B.
At step 630 , the processed capture probe precursors are ligated to generate head-to-tail concatemers 240 , as illustrated in FIG. 4 , step C. As illustrated in FIG. 4 , step C, it is noted that the ligation reaction naturally drives towards the formation of circularized templates due to the fact that in very dilute solutions of free ends, the probability of self-ligation (circularization) becomes higher than the probability of finding a separate free end.
At step 640 , the head-to-tail concatemers 240 are amplified using any suitable amplification method, such as PCR amplification, in vitro transcription, Klenow, or isothermal amplification.
In one embodiment, the amplification of the head-to-tail concatemers 240 is carried out using isothermal amplification with either Bacillus subtilis phage phi29 polymerase (hereafter referred to as “phi29” polymerase) or Bacillus stearothermophilus (Bst) DNA polymerase large fragment, 5′→3′ exo − (hereafter referred to as “Bst DNA polymerase”). Isothermal amplification is based on random priming of denatured DNA, followed by strand-displacement synthesis at constant temperature, wherein multiple primers are extended over tens of kilobases, as described in Lage et al., Genome Res 13:294-307 (2003), incorporated herein by reference. The single-stranded DNA generated by strand displacement is targeted by new random priming events, and these new strands are elongated in the opposite direction, resulting in a hyperbranched network of amplified head-to-tail concatemers, as shown in FIG. 4 , step D.
In one embodiment, as illustrated in FIG. 4 , step D, a random primer 250 is used for amplification using the strand displacement polymerase technology found in the TEMPLIPHI isothermal amplification kit (phi29) (GE Life Sciences, Piscataway N.J.). In one embodiment, the random primer 250 comprises a random 7-mer amplification primer with an additional two nitroindole residues at the 5′ end and a phosphorothioate linkage at the 3′ end: 5′[nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77), as described by Lage et al., Genome Res 13:294-307 (2003).
At step 650 , the amplified head-to-tail concatemers are processed to release monomer double-stranded capture probe precursors. In one embodiment, as shown in FIG. 4 , step E, the concatemers are processed by restriction enzyme cleavage using a type II restriction enzyme that recognizes a site present in the 5′ and 3′ flanking regions, such as Bsm1.
At step 660 , the amplified monomer double-stranded precursor molecules are processed to remove the 5′ and/or 3′ flanking regions. In one embodiment, as shown in FIG. 4 , step E and step F, the 5′ flanking region is precisely removed by digestion with Bsm1 and the 3′ flanking region is precisely removed by digestion with either Psi1 or HindIII.
At step 670 , the monomer double-stranded precursor molecules are further processed to selectively remove the complementary strand of the capture probe to produce a population of single-stranded target specific capture probes 200 .
In one embodiment, as shown in FIG. 4 , at step E, the Bsm1 digested monomers are treated with alkaline phosphatase to remove the 5′ terminal phosphates. At step F, the precise 3′ ends of capture probes are then liberated by digestion with either PsiI or HindIII, which each leaves 5′ terminal phosphates on the complementary strands. At step G, single-strand capture probe 200 libraries containing only the target specific region 202 and the capture reagent binding region 204 are generated by digesting the double-stranded monomer capture probe precursors with an enzyme, such as Lambda exonuclease, that specifically degrades dsDNA by attacking at 5′ phosphate sites and selectively digests away the non-capture complementary strand, thereby converting the dsDNA into ssDNA suitable for use as capture probes.
Alternatively, the non-capture complementary strand may be removed by first adding exonuclease resistant adaptors to the capture probe strand and degrading away the non-capture complementary strand with any suitable double-strand DNA specific exonuclease, such as Exonuclease III. In another example, the capture strand may be selectively amplified by adding an amplification primer binding site in the 5′ flanking region 210 of the capture probe precursor 230 , and selectively amplifying the plurality of amplified capture probe monomers 270 with an amplification primer that binds to the amplification primer binding site.
FIG. 5 illustrates further details of the method illustrated in FIG. 4 , with regard to a library of single-stranded nucleic acid probes having distinct predetermined sequences A, B, C, and D, such as target-specific capture probes. It will be understood that the method of generating the library comprising probes A, B, C and D is representative for generating a library comprising at least 1,000, such as at least 5,000, such as at least 10,000, such as at least 50,000, such as at least 100,000, such as at least 1,000,000 or more single-stranded nucleic acid probes having predetermined nucleic acid sequences, such as target-specific capture probes. As shown in FIG. 5 , step A, a library of single-stranded precursor molecules 100 is synthesized on a substrate 102 , the synthesized population comprising a 5′ flanking region 210 , a target specific binding region 202 and a portion of the capture reagent binding region 204 , wherein each of the target specific binding regions comprises a nucleic acid sequence selected to bind to target sequence A, B, C, or D.
The synthesized library of single-stranded precursor molecules 100 is then cleaved off the substrate 102 , annealed to a common reverse primer 150 and extended with Klenow, to generate a library of double-stranded capture probe precursor molecules 230 (best illustrated in FIG. 4 ). Accordingly, the library of double-stranded nucleic acid precursor molecules 230 comprises a 5′ flanking region 210 that is essentially identical to every other 5′ flanking region in the library, a 3′ flanking region 220 that is essentially identical to every other 3′ flanking region in the library, and a probe region 200 comprising a nucleic acid sequence 202 that is different from a least a portion of the nucleic acid sequence 202 present in every other probe region 200 .
Referring again to FIG. 5 , at step C, the 5′ and 3′ regions of the double-stranded capture probe precursor molecules 230 are then processed to generate ligation substrates and ligated to form head-to-tail concatemers 240 as described with reference to FIG. 4 . As illustrated in FIG. 5 , step C, the head-to-tail concatemers 240 each comprise a plurality of ligated monomer capture probe regions (A, B, C, D) that are randomly ligated together. At step D, the circularized concatemers 240 are amplified via isothermal amplification with random primers 250, resulting in a plurality of amplified concatemers 260 (best shown in FIG. 4 ), which are then processed (e.g., with Bsm1) at Step E to generate a library of amplified capture probe monomers 270 , which are further processed to remove the non-capture complementary strands, as described with reference to FIG. 4 , into a library of single-stranded capture probes 200 comprising a plurality of different target specific binding regions 202 (e.g., that specifically bind to targets A, B, C, and D).
In some embodiments of the methods described herein, a library of capture probe precursors in the form of head-to-tail concatemers, with reference to FIG. 4 , step C (e.g., the ligation mixture), or amplified head-to-tail concatemers, with reference to FIG. 4 , step D (e.g., the amplified reaction) may be stored at −20° C. for a period of time from several hours up to 6 months or longer, for use as a template in a subsequent amplification reaction, in order to generate additional yields of the capture probe library 200 , thus avoiding the need for repeated oligonucleotide synthesis of a particular library of capture probes.
The population of single-stranded capture probes 200 can be used in solution based capture methods as described herein. As demonstrated in Example 2, and shown in FIG. 6 , it has been determined that the single-stranded capture probes generated from amplified head-to-tail concatemers, in accordance with the methods of the invention, work at least as well for solution based capture methods as a population of corresponding capture probes that were generated by direct oligonucleotide synthesis. Therefore, it is demonstrated that the methods are useful for generating a library of uniformly amplified single-stranded capture probes from a starting population of double-stranded capture probe precursor molecules.
The level of representation of expected nucleic acid sequences in a library generated according to the methods of the invention typically has a variation of less than about 30% (such as a variation of less than about 20%). The level of representation of expected nucleic acid sequence in the final single stranded capture probe library 200 may be assessed using various methods. For example, as described in Example 2, a capture probe library 200 may be used for solution-based capture of a set of targets (e.g., the 13 exons of the AKT gene), and the standard deviation of the exon to exon capture efficiency, expressed as a percentage of the fold-enrichment can be determined, as shown in FIG. 6 , thereby providing an indirect measure of the representation of the expected nucleic acid sequences in the capture probe library 200 . As another example, quantitative PCR assays may be carried out for representative target sequences at an early step in the method shown in FIG. 4 , such as after initial synthesis of the single-stranded precursor nucleic acid molecules 100 , or after formation of the double-stranded precursor molecules 230 , and compared to quantitative PCR results obtained from the final population of single-stranded capture probes 200 to verify uniform representation of the target sequence. As another example, a test sample that is representative of the final population of single-stranded capture probes 200 can be labeled and hybridized to a substrate comprising a population of nucleic acid molecules comprising the set of predetermined sequences expected to be present in the final population of single-stranded capture probes 200 , and the representation of the expected nucleic acids in the test sample is evaluated by analyzing the resulting hybridization pattern.
Oligonucleotide Synthesis
DNA synthesis of the various oligonucleotides of the invention (e.g., single-stranded nucleic acid molecules having predetermined sequences, capture probe precursors and universal adaptor oligonucleotides) can be carried out by any art-recognized chemistry, including phosphodiester, phosphotriester, phosphate triester, or N-phosphonate and phosphoramidite chemistries (see, e.g., Froehler et al., Nucleic Acid Res. 14:5399-5407, 1986; McBride et al., Tetrahedron Lett. 24:246-248, 1983). Methods of oligonucleotide synthesis are well known in the art and generally involve coupling an activated phosphorous derivative on the 3′ hydroxyl group of a nucleotide with the 5′ hydroxyl group of the nucleic acid molecule (see, e.g., Gait, Oligonucleotide Synthesis: A Practical Approach, IRL Press, 1984).
A population of nucleic acid molecules can be synthesized on a substrate by any art-recognized means including, for example, photolithography (see, Lipshutz et al., Nat. Genet. 21(1 Suppl):20-24, 1999) and piezoelectric printing (see, Blanchard et al., Biosensors and Bioelectronics 11:687-690, 1996). In some embodiments, nucleic acid molecules are synthesized in a defined pattern on a solid substrate to form a high-density microarray. Techniques are known for producing arrays containing thousands of oligonucleotides comprising defined sequences at defined locations on a substrate (see, e.g., Pease et al., Proc. Nat'l. Acad. Sci. 91:5022-5026, 1994; Lockhart et al., Nature Biotechnol. 14:1675-80, 1996; and Lipshutz et al., Nat. Genet. 21 (1 Suppl):20-4, 1999).
In some embodiments, populations of nucleic acid molecules are synthesized on a substrate, to form a high density microarray, by means of an ink jet printing device for oligonucleotide synthesis, such as described by Blanchard in U.S. Pat. No. 6,028,189; Blanchard et al., Biosensors and Bioelectrics 11:687-690 (1996); Blanchard, Synthetic DNA Arrays in Genetic Engineering, Vol. 20, J. K. Setlow, Ed. Plenum Press, New York at pages 111-123; and U.S. Pat. No. 6,028,189 issued to Blanchard. The nucleic acid sequences in such microarrays are typically synthesized in arrays, for example, on a glass slide, by serially depositing individual nucleotide bases in “microdroplets” of a high surface tension solvent such as propylene carbonate. The microdroplets have small volumes (e.g., 100 picoliters (pL) or less, or 50 pL or less) and are separated from each other on the microarray (e.g., by hydrophobic domains) to form surface tension wells which define the areas containing the array elements (i.e., the different populations of nucleic acid molecules). Microarrays manufactured by this ink-jet method are typically of high density, typically having a density of at least about 2,000 different nucleic acid molecules per 1 cm 2 . The nucleic acid molecules may be covalently attached directly to the substrate, or to a linker attached to the substrate at either the 3′ or 5′ end of the polynucleotide. Exemplary chain lengths of the synthesized nucleic acid molecules suitable for use in the present methods are in the range of about 20 to about 100 nucleotides in length, such as 50 to 100, 60 to 100, 70 to 100, 80 to 100, or 90 to 100 nucleotides in length. In some embodiments, the nucleic acid molecules are in the range of 80 to 100 nucleotides in length.
Exemplary ink jet printing devices suitable for oligonucleotide synthesis in the practice of the present invention contain microfabricated ink-jet pumps, or nozzles, which are used to deliver specified volumes of synthesis reagents to an array of surface tension wells (see, Kyser et al., J. Appl. Photographic Eng. 7:73-79, 1981).
In some embodiments, a population of nucleic acid molecules is synthesized to form a high-density microarray. A DNA microarray, or chip, is an array of nucleic acid molecules, such as synthetic oligonucleotides, disposed in a defined pattern onto defined areas of a solid support (see, Schena, BioEssays 18:427, 1996). The arrays are preferably reproducible, allowing multiple copies of a given array to be produced and easily compared with each other. Microarrays are typically made from materials that are stable under nucleic acid molecule hybridization conditions. In some embodiments, the nucleic acid molecules on the array are single-stranded DNA sequences. Exemplary microarrays and methods for their manufacture and use are set forth in T. R. Hughes et al., Nature Biotechnology 19:342-347, April 2001, which publication is incorporated herein by reference.
In some embodiments, the methods of the invention utilizes oligonucleotides that are synthesized on a multiplex parallel DNA synthesis system based on an integrated microfluidic microarray platform for parallel production of oligonucleotides, wherein the DNA synthesis system utilizes photogenerated acid chemistry, parallel microfluidics and a programmable digital light controlled synthesizer, as described in U.S. Patent Pub. No. 2007/0059692, Gao et al., Biopolymers 73:579-596 (2004), and Zhou et al., Nucleic Acids Research 32(18):5409-5417 (2004), each of which is incorporated herein by reference.
In some embodiments, the methods of the invention utilize synthesized oligonucleotides that are cleaved off a substrate, such as a microarray. The synthesized nucleic acid molecules can be harvested from the substrate by any useful means. In some embodiments, the portion of the nucleic acid molecule that is directly attached to the substrate, or attached to a linker that is attached to the substrate, is attached to the substrate or linker by an ester bond which is susceptible to hydrolysis by exposure to a hydrolyzing agent, such as hydroxide ions, for example, an aqueous solution of sodium hydroxide or ammonium hydroxide. The entire substrate can be treated with a hydrolyzing agent, or alternatively, a hydrolyzing agent can be applied to a portion of the substrate. For example, a silane linker can be cleaved by exposure of the silica surface to ammonium hydroxide, yielding various silicate salts and releasing the nucleic acid molecules with the silane linker into solution. In some embodiments, ammonium hydroxide can be applied to the portion of a substrate that is covalently attached to the nucleic acid molecules, thereby releasing the nucleic acid molecules into the solution (see, Scott and McLean, Innovations and Perspectives in Solid Phase Synthesis, 3 rd International Symposium, 1994, Mayflower Worldwide, pp. 115-124).
In another aspect, the present invention provides a method for enriching a library for target nucleic acid regions of interest. The methods according to this aspect of the invention comprise: (a) amplifying a plurality of head-to-tail concatemers formed from ligating a population of double-stranded nucleic acid precursor molecules, wherein each double-stranded precursor molecule in the starting population comprises a target capture probe region comprising (i) a target-specific binding region comprising a nucleic acid sequence that is at least 95% identical to at least a portion of the sense or antisense strand of a target nucleic acid sequence of interest and (ii) a region for binding to a capture reagent; wherein the target capture region is flanked on the 5′ end by a 5′ flanking region comprising a first processing site and is flanked on the 3′ end by a 3′ flanking region comprising a second processing site; (b) processing the amplified head-to-tail concatemers to release double-stranded monomer precursor molecules; (c) selectively removing the complement strand of the double-stranded monomer precursor molecules to generate a population of single-stranded capture probes, each capture probe comprising (i) a target-specific binding region comprising a nucleic acid sequence that is at least 95% identical to at least a portion of the sense or antisense strand of a target nucleic acid sequence of interest and (ii) a region for binding to a capture reagent; (d) contacting the population of single-stranded capture probes with a library comprising at least one target nucleic acid sequence of interest under conditions that allow binding between the capture probes and the at least one nucleic acid target region of interest, to form a mixture comprising a plurality of complexes between target regions of interest and capture probes; (e) contacting the mixture of step (d) with a capture reagent and separating the capture reagent bound complex from the mixture; and (f) eluting the target regions of interest from the capture reagent bound complex.
The steps (a) to (c) may be carried out as previously herein described. The steps (d) to (f) of enriching a library for target sequences with the population of single-stranded capture probes may be carried out as illustrated in FIG. 2 . As shown in FIG. 2A , solution-based capture is carried out by first annealing the library of single-stranded capture probes 200 , each capture probe comprising a target specific region 202 that hybridizes to a target sequence contained in a library insert, with a library of nucleic acid molecules 50 comprising nucleic acid target insert sequences of interest 10 flanked by a first primer binding region 22 on one end and a second primer binding region 32 on the other end. As further shown in FIG. 2 , step A, in one embodiment, the library of nucleic acid molecules 50 is annealed with a combination of a library of single-stranded capture probes 200 each comprising a region 204 that hybridizes to a universal adaptor oligo 300 and an equimolar amount of universal adaptor oligos 300 comprising a moiety 310 for binding to a capture reagent 400 .
The annealing step is typically carried out by mixing a molar excess of capture probes (or capture probes plus universal adaptor oligos) with the library in a high salt solution comprising from 100 mM to 2 M NaCl (osmolarity=200 to 4000 molar). An exemplary high salt solution for annealing is 10 mM Tris pH 7.6, 0.1 mM EDTA, 1 M NaCl (osmolarity=2000 molar). The nucleic acid molecules in the mixture are then denatured (i.e., by heating to 94 degrees) and allowed to cool to room temperature. In one embodiment, the annealing step is carried out in a high salt solution comprising from 100 mM to 2 M NaCl with the addition of 0.1% triton X100 (or Tween or NP40) nonionic detergent.
An amount of capture reagent 400 is added to the annealed mixture sufficient to generate a plurality of complexes each containing a nucleic acid molecule, a capture probe (or a capture probe and a universal adaptor oligo), and a capture reagent. This step is carried out in a high salt solution comprising from 100 mM to 2 M NaCl (osmolarity=200 to 4000 molar). An exemplary high salt solution for anneal is 10 mM Tris pH 7.6, 0.1 mM EDTA, 1 M NaCl (osmolarity=2000 molar). The mixture is incubated at room temperature with mixing for about 15 minutes.
The complexes formed are then isolated or separated from solution with a sorting device 500 (e.g., a magnet) that pulls or sorts the capture reagent 400 out of solution.
The sorted complexes bound to the capture reagent 400 are washed with a low salt wash buffer (less than 10 mM NaCl, and more preferably no NaCl) to remove non-target nucleic acids. An exemplary low salt wash buffer is 10 mM Tris pH 7.6, 0.1 mM EDTA (osmolarity=10 millimolar). In some embodiments, the low salt wash optionally contains from 15% to 30% formamide, such as 25% formamide (osmolarity=6.3 molar). For each wash step, the capture reagent 400 bound to the complexes (e.g., magnetic beads) are resuspended in the low salt wash buffer and rocked for 5 minutes, then sorted again with the sorting device (magnet). The wash step may be repeated 2 to 4 times.
The nucleic acid molecules containing the target sequences are then eluted from the complexes bound to the capture reagent as follows. The washed complexes bound to the capture reagent 400 are resuspended in water, or in a low salt buffer (i.e., osmolarity less than 100 millimolar), heated to 94° C. for 30 seconds, the capture reagent (e.g., magnetic beads) is pulled out using a sorting device (e.g., magnet), and the supernatant (eluate) containing the target nucleic acid molecules is collected.
The eluate may optionally be amplified in a PCR reaction with a first PCR primer that binds to the first primer binding site 22 in the first linker and a second PCR primer that binds to the second primer binding site 32 in the second linker, producing an enriched library which can be optionally sequenced.
In another aspect, the present invention provides kits for generating a population of single-stranded nucleic acid molecules from a population of precursor double-stranded molecules. The kits according to this aspect of the invention are useful for carrying out various embodiments of the methods of the invention described herein. The kits in accordance with this aspect of the invention comprise (a) a plurality of random 7-mer oligonucleotide primers, (b) at least one of phi29 polymerase or Bst DNA polymerase large fragment 5′-3′ exo-; and (c) a lambda exonuclease enzyme. In some embodiments of the kit, the plurality of random 7-mer oligonucleotide primers each comprise an additional two nitroindole residues at the 5′ end and a phosphorothioate linkage at the 3′ end (SEQ ID NO:77). In some embodiments, the kit may further comprise at least one of the following: an alkaline phosphatase enzyme, at least one type II restriction enzyme, a DNA ligase, and a DNA polymerase enzyme (Klenow).
In an embodiment of the kit comprising phi29 polymerase, the kit may optionally further comprise one or more of the following reagents: (i) a phi29 concentrated stock reaction buffer comprising at least one of the following: Tris-HCL (e.g., at 50 mM for 10× buffer), (NH 4 ) 2 SO 4 (e.g., at 10 mM for 10× buffer), MgCl 2 (e.g., 10 mM for 10× buffer) and dithiothreitol (e.g., 4 mM for 10× buffer); (ii) a concentrated stock of dNTPs (e.g. from 100 μM to 10 mM dNTPs); and (iii) a reducing agent, such as dithiothreitol. In further embodiments, the kit may optionally comprise at least one or more of the following; a common reverse primer oligo 150 designed to hybridize to the region 204 for binding to the synthesized oligos 100 , Klenow enzyme, at least one Type II Restriction Enzyme, ligase, and alkaline phosphatase.
The following examples merely illustrate the best mode now contemplated for practicing the invention, but should not be construed to limit the invention.
EXAMPLES
All of the compositions and methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. In some cases, the compositions and methods of this invention have been described in terms of embodiments, however these embodiments are in no way intended to limit the scope of the claims, and it will be apparent to those of skill in the art that variations may be applied to the compositions and/or methods and in the steps or in the sequence of steps of the methods described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain components which are both chemically and physiologically related may be substituted for the components described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.
Example 1
This Example demonstrates a method for uniformly amplifying a library of target capture probes specific for the AKT gene designed for solution based capture of the 13 exons of the AKT gene.
Rationale:
Several recent studies describe the complete resequencing of human genomes (Wang et al., Nature 456:60 (2008); Bentley et al., Nature 456:53 (2008); Ley et al., Nature 456:66 (2008)). One in particular describes the full genome sequence of a tumor and normal adjacent tissue (Ley et al. (2008)). Remarkably, the authors distill their analysis of the complete human genome to ten mutated genes, highlighting the point that a small percentage of human genome sequence variations, including the protein coding regions and some surrounding flanking sequences, is interpretable. At the opposite end of the spectrum, candidate gene resequencing strives to test phenotype to genotype hypotheses by seeking sequence variation in specific genes that are thought to influence traits. The caveat is that the selection of candidate genes can be somewhat arbitrary. The ideal solution is one in which the interpretable regions, referred to as the “exome” of the genome, can be selectively resequenced. As used herein, the term “exome” refers to the collection of genomic segments that include protein coding regions, exons, promoters, known ncRNAs (non-coding RNAs) and UTRs, altogether comprising about 2% of the human genome. Interestingly, if the exome could be captured from a sample, then the sequencing data from a single flow cell from a highly parallel sequencing technology (e.g., a single Illumina GAII flow cell) would be adequate to address all of the diploid variation present in the exomic fraction of that sample.
Solution-based capture of the human exome would require ˜2 million oligonucleotides. Even with state-of-the-art, high-throughput oligo synthesis stations, the cost of each capture oligo is $7 and therefore the cost of synthesizing an exome solution based capture library would be approximately $14 million. An alternative—synthesized oligonucleotide libraries cleaved from microarrays, also initially appears to be an untenable solution due to the high cost involved to obtain sufficient yields of material. Each microarray typically produces about 50,000 individual oligonucleotide sequences that are high-quality, cleavable oligos in picomole quantities (e.g., commercially available from Agilent, Santa Clara, Calif.). Thus, the cost of synthesizing a library of exome capture oligonucleotide probes from microarrays (requiring ˜40 arrays at an estimated cost of $400,000) is substantially reduced relative to conventional oligonucleotide synthesis, however, the yield of material produced by microarray synthesis is sufficient for only four solution based capture experiments. Moreover, the use of high density probe coverage (i.e., more than 4 probes per exon, or at least one capture probe per 35 nt), as shown in FIG. 1B , is preferable for solution based capture in comparison to low density probe coverage (i.e., less than 3 probes per exon, or less than one capture probe per 35 nt), as shown in FIG. 1A . The use of high density probe coverage is preferred in order to ensure that desired targets are enriched, thereby reducing the depth of re-sequencing required of the enriched material. However, such high density probe coverage calls for a greater number of capture oligonucleotides, which significantly inflates the overall expense of technologies that utilize solution based capture methods. Therefore, a need exists to provide a cost-effective method for generating high quality custom oligonucleotide libraries with sufficient yield for applications such as solution based capture.
This Example demonstrates a method for uniformly amplifying a library of synthesized capture oligonucleotides for use in solution based capture methods.
Design of Capture Probe Oligonucleotide Precursors for Amplification and Processing into a Library of Capture Probes:
As shown in FIG. 1C , capture probes 200 may be used to capture nucleic acid molecules comprising target sequences 10 from a mixture of target and non-target nucleic acid molecules. As shown in FIG. 1C , capture probes 200 comprise a target-specific binding region 202 and a region 204 for binding to a capture reagent 300 . In the embodiment shown in FIG. 1C , the capture reagent 300 is a universal adaptor oligonucleotide comprising a moiety 310 that binds to a capture reagent, thereby resulting in a tri-molecular solution based capture complex.
Design of Capture Probe Precursor Oligonucleotides:
FIG. 4 at Step A illustrates the general structure of a double-stranded capture probe precursor 230 prior to processing into a single-stranded capture probe 200 . The double-stranded capture probe precursor was designed to ensure that the ligation of a plurality of double-stranded capture oligonucleotide probe precursors 230 would result in head-to-tail concatemers 240 , as illustrated at Step C, which were then used as templates for amplification, as illustrated at Step D, followed by cleavage into monomers and alkaline phosphatase, as illustrated at Step E, followed by selective degradation of the complementary strand of the capture probe precursor, as shown at Step G, thereby generating a single-stranded capture probe 200 comprising a target-specific binding region 202 and a region 204 for binding to a capture reagent 300 , without the 5′ flanking region 210 and the 3′ flanking region 220 .
As shown in FIG. 4 , Step A, the general structure of the double-stranded capture probe precursor 230 includes a 5′ flanking region 210 comprising a first restriction enzyme site for creating a first nucleotide overhang for ligation (e.g., Bsm1); a capture probe region 200 comprising a target specific hybridizing region 202 and a universal capture oligo hybridizing region 204 , and a 3′ flanking region 220 comprising a second restriction enzyme site (e.g., Bsm1) for creating a second nucleotide overhang for ligation, and further comprising a third restriction enzyme site (e.g., Psi or HindIII), for precisely cleaving off the 3′ flanking region.
Design of the Target-Specific Region 202 of the Capture Probe 200 :
In this Example, a 64 oligonucleotide library was synthesized for high density solution based capture of the 13 exon AKT1 gene (NM — 005163). As illustrated in FIG. 1B , a library of oligonucleotide capture probes were designed such that each target AKT1 exon had at least 4 or more probes (high density), and the capture probes alternated in strand orientation and were perfectly head to tail with no spaces in between, each having the sequence identical to the corresponding exon sequences 1-13 from the AKT1 gene. The target specific binding regions 202 of the capture probes in the AKT1 capture probe library had a length of 35 nt, as shown below in TABLE 1.
Design of the Region 204 for Binding to a Capture Reagent 300 :
The AKT1 capture probes 200 in the library were designed such that each final processed single-stranded capture probe had a 34 nucleotide common region 204 for binding to the universal oligo adaptor capture reagent 300 .
As shown in FIG. 4 , Step A, in order to further reduce costs of oligonucleotide synthesis, in this Example, the initial population of synthesized oligos 100 included the 5′ flanking region 210 , the target specific binding regions 202 , and only a portion (15 nt of the 34 nt region) of the common region 204 for binding to a capture reagent 300 . As shown in FIG. 4 , Step A, the 15 nt of the region 204 (5′ ACGCGTGGCGGATGT 3′ (SEQ ID NO:1)) binds to a region of the reverse primer 150 that was used to anneal and fill-in the library synthesized oligos 100 , thereby resulting in the double-stranded capture probe precursors 230 .
Design of the 5′ Flanking Region 210 of the Capture Probe Precursor 230 :
The double-stranded AKT1 capture probe precursors 230 were designed to include a 5′ flanking region 210 comprising a first restriction enzyme site for Bsm1, in order to create a two nucleotide “GG” overhang at the first end of the precursor molecules 230 after digestion with Bsm1 to facilitate ligation into head-to-tail concatemers.
The 5′ flanking region 210 of the capture probe precursors in this Example had the following sequence: 5′ CGCGAATGCC 3′ (SEQ ID NO:2) to provide a first Bsm1 site.
Design of the 3′ Flanking Region 220 of the Capture Probe Precursor 230 :
The double-stranded AKT1 capture probe precursors 230 were designed to include a 3′ flanking region 220 comprising a second restriction enzyme site for Bsm1, in order to create a two nucleotide “CC” overhang at the second end of the precursor 230 molecule after digestion with Bsm1 to facilitate ligation into head-to-tail concatemers.
The 3′ flanking region was designed to also include a third restriction enzyme recognition sequence to precisely cleave off the 3′ flanking region after amplification, and also leave 5′ terminal phosphates on the complementary strands (e.g., Psi1 or HindIII).
Using the general design principles described above, several series of oligonucleotides were synthesized as follows:
“A” Series: a control set of 64 AKT capture probes that were directly synthesized (not amplified) having a total length of 69 nucleotides.
“B” Series: a set of 64 AKT capture probe precursor oligonucleotides for amplification, but which did not impose the head to tail ligation characteristics (later abandoned, as discussed below in the results section).
“C” Series: a set of 64 AKT capture probe precursor oligonucleotides for amplification, having a total length of 79 nucleotides (34 nt 204 region that binds to the reverse primer 150 , with the same 5′ flanking region 210 and target specific region 202 as D series). Note: this series was more expensive to synthesize because they required 0.2 micromolar synthesis, due to the longer size.
“D” Series: a set of 64 AKT capture probe precursor oligonucleotides for amplification, having a total length of 60 nucleotides, including a 15 nt 204 region that binds to the reverse primer 150 , with a 10 nucleotide 5′ flanking region 210 and a 35 nucleotide target-specific region 202 . Note: this series was much less expensive because it was synthesized on a 50 nmole scale, due to shorter size, which was then extended with the reverse primer and Klenow fill-in reaction.
As described above, the C and D series oligos were designed such that after the fill-in reaction with the common reverse primer 150 , a pair of asymmetric Bsm1 sites were created on the double-stranded precursor molecule 230 , such that following digestion with Bsm1, a two nucleotide “CC” overhang is present on the top strand of each double-stranded oligonucleotide and a two nucleotide “GG” overhang is present on the bottom strand of each double-stranded oligonucleotide, thus creating a situation in which only head-to-tail ligation events are allowed. Moreover, ligation recreates the Bsm1 site for downstream processing after amplification. As described in detail below, the Bsm1 digested amplification products were then treated with Antarctic phosphatase to dephosphorylate the 5′ end of the capture strand while leaving an exposed 5′ phosphate on the complement strand to allow for degradation by treatment with lambda exonuclease, to generate the desired single-stranded capture probes.
For the “D” Series oligos each capture probe precursor 100 was synthesized as shown below in TABLE 1, with the 5′ regions 210 and the 3′ regions 220 underlined.





TABLE 1



The D Series probes for AKT (NM_005163)









Distance


SEQ



from exon


ID

Exon
5′ edge
Strand
Probe Sequence
NO:










1
−35
−
CGCGAATGCC GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCT ACG
3






CGTGGCGGATGT




1
0
+
CGCGAATGCC ATGAGCGACGTGGCTATTGTGAAGGAGGGTTGGCT ACG
4





CGTGGCGGATGT




1
35
−
CGCGAATGCC CCAGCCCTGGCAGCGGGTACTAACCTCGTTTGTGC ACG
5





CGTGGCGGATGT




1
70
+
CGCGAATGCC GCCTGGGGAGGGAGAGATGGGGGTAGTAGCCCCAG ACG
6





CGTGGCGGATGT




2
−35
−
CGCGAATGCC CTACAGACGTGCGGGTGGTGAGAGCCACGCACACT ACG
7





CGTGGCGGATGT




2
0
+
CGCGAATGCC GGGAGTACATCAAGACCTGGCGGCCACGCTACTTC ACG
8





CGTGGCGGATGT




2
35
−
CGCGAATGCC TTGTAGCCAATGAAGGTGCCATCATTCTTGAGGAG ACG
9





CGTGGCGGATGT




2
70
+
CGCGAATGCC GGAGCGGCCGCAGGATGTGGACCAACGTGAGGCTC ACG
10





CGTGGCGGATGT




2
105
−
CGCGAATGCC GGGATACTTACGCGCCACAGAGAAGTTGTTGAGGG ACG
11





CGTGGCGGATGT




2
140
+
CGCGAATGCC CTTGGCCTCTCGGGATTCAGATTTGGGGGGTTGGC ACG
12





CGTGGCGGATGT




3
−35
−
CGCGAATGCC CTGCGGGCAGGCAGAGCCTCTGTCTGCGTGCATCCACG
13




CGTGGCGGATGT



3
0
+
CGCGAATGCCAGTGCCAGCTGATGAAGACGGAGCGGCCCCGGCCC ACG
14





CGTGGCGGATGT




3
35
−
CGCGAATGCC GTGGTCCACTGCAGGCAGCGGATGATGAAGGTGTT ACG
15





CGTGGCGGATGT




3
70
+
CGCGAATGCC TGTCATCGAACGCACCTTCCATGTGGAGACTCCTG ACG
16





CGTGGCGGATGT




3
105
−
CGCGAATGCC CCTGGCCTGGCCGCCACAGCCCACGTACCGCTCCT ACG
17





CGTGGCGGATGT




4
−35
−
CGCGAATGCC CTGCAGGAGGTCAGGTGAGGCTGCAGGCCTGTACC ACG
18





CGTGGCGGATGT




4
0
+
CGCGAATGCC GGAGGAGTGGACAACCGCCATCCAGACTGTGGCTG ACG
19





CGTGGCGGATGT




4
35
−
CGCGAATGCC GTCCATCTCCTCCTCCTCCTGCTTCTTGAGGCCGT ACG
20





CGTGGCGGATGT




4
70
+
CGCGAATGCC TTCCGGTCGGGCTCACCCAGTGACAACTCAGGGGC ACG
21





CGTGGCGGATGT




4
105
−
CGCGAATGCC GCTTGGGCTTGGCCAGGGACACCTCCATCTCTTCA ACG
22





CGTGGCGGATGT




4
140
+
CGCGAATGCC ACCGCGTGGTGAGGCCTGTCCCCACTTCTGCCTGT ACG
23





CGTGGCGGATGT




5
−35
−
CGCGAATGCC CTATGGGCAGGCACCAGGGTCAGCAAGCGGCGCTG ACG
24





CGTGGCGGATGT




5
0
+
CGCGAATGCC ACCATGAACGAGTTTGAGTACCTGAAGCTGCTGGG ACG
25





CGTGGCGGATGT




5
35
−
CGCGAATGCC CCTTCACCAGGATCACCTTGCCGAAAGTGCCCTTG ACG
26





CGTGGCGGATGT




5
70
+
CGCGAATGCC AGAAGGCCACAGGCCGCTACTACGCCATGAAGATC ACG
27





CGTGGCGGATGT




5
105
−
CGCGAATGCC GGCCCCACCTTGGCCACGATGACTTCCTTCTTGAG ACG
28





CGTGGCGGATGT




6
−35
−
CGCGAATGCC CTGTAAAGCAGGGCTGGGTGAGCTGCCACCCCGCA ACG
29





CGTGGCGGATGT




6
0
+
CGCGAATGCC GACGAGGTGGCCCACACACTCACCGAGAACCGCGT ACG
30





CGTGGCGGATGT




6
35
−
CGCGAATGCC TCACTGTGAGGAAGGGGTGCCTGGAGTTCTGCAGG ACG
31





CGTGGCGGATGT




6
70
+
CGCGAATGCC GTGGGAGCCCAGATGGGGCTGAAGGGCTGGGGCCA ACG
32





CGTGGCGGATGT




7
−35
−
CGCGAATGCC CTGCAAGGAAGGGGAGCTGGAACTGCGGCCCCACA ACG
33





CGTGGCGGATGT




7
0
+
CGCGAATGCC GCCCTGAAGTACTCTTTCCAGACCCACGACCGCCT ACG
34





CGTGGCGGATGT




7
35
−
CGCGAATGCC CCTCGCCCCCGTTGGCGTACTCCATGACAAAGCAG ACG
35





CGTGGCGGATGT




7
70
+
CGCGAATGCC TAGGGGCTGGGGCTGCGGGGGATGGACTTCGCGGC ACG
36





CGTGGCGGATGT




8
−35
−
CGCGAATGCC CTGCGGGAGGCGCAACCTGAGGCACAGCCGTGGCT ACG
37





CGTGGCGGATGT




8
0
+
CGCGAATGCC CTGTTCTTCCACCTGTCCCGGGAGCGTGTGTTCTC ACG
38





CGTGGCGGATGT




8
35
−
CGCGAATGCC CAATCTCAGCGCCATAGAAGCGGGCCCGGTCCTCG ACG
39





CGTGGCGGATGT




8
70
+
CGCGAATGCC TGTCAGCCCTGGACTACCTGCACTCGGAGAAGAAC ACG
40





CGTGGCGGATGT




8
105
−
CGCGAATGCC GCCCGCCAGCGCACCTTGAGGTCCCGGTACACCAC ACG
41





CGTGGCGGATGT




9
−35
−
CGCGAATGCC CTAGGGGAAAGGTGGCCTCAGGTCAGTGCCGCCAG ACG
42





CGTGGCGGATGT




9
0
+
CGCGAATGCC CTGGAGAACCTCATGCTGGACAAGGACGGGCACAT ACG
43





CGTGGCGGATGT




9
35
−
CGCGAATGCC TCCCCTCCTTGCACAGCCCGAAGTCTGTGATCTTA ACG
44





CGTGGCGGATGT




9
70
+
CGCGAATGCC TCAAGGACGGTGCCACCATGAAGACCTTTTGCGGC ACG
45





CGTGGCGGATGT




9
105
−
CGCGAATGCC GGGGCGCACACCTCGGGGGCCAGGTACTCAGGTGT ACG
46





CGTGGCGGATGT




10
−35
−
CGCGAATGCC CTGCACGGGTGGCAGATGGGCAGGACTCGGCATCA ACG
47





CGTGGCGGATGT




10
0
+
CGCGAATGCC GTGCTGGAGGACAATGACTACGGCCGTGCAGTGGA ACG
48





CGTGGCGGATGT




10
35
−
CGCGAATGCC TCATCTCGTACATGACCACGCCCAGCCCCCACCAG ACG
49





CGTGGCGGATGT




10
70
+
CGCGAATGCC TGTGCGGTCGCCTGCCCTTCTACAACCAGGACCAT ACG
50





CGTGGCGGATGT




10
105
−
CGCGAATGCC ATCTCCTCCATGAGGATGAGCTCAAAAAGCTTCTC ACG
51





CGTGGCGGATGT




10
140
+
CGCGAATGCC CCGCTTCCCGCGCACGCTTGGTCCCGAGGCCAAGT ACG
52





CGTGGCGGATGT




10
175
−
CGCGAATGCC CTTGGGGTCCTTCTTGAGCAGCCCTGAAAGCAAGG ACG
53





CGTGGCGGATGT




10
210
+
CGCGAATGCC CAGAGGTGAGGGCCGCCCATCCCAGCTACAGGCTA ACG
54





CGTGGCGGATGT




11
−35
−
CGCGAATGCC CTGCAGGCAGGAAACAAGGCCACAGTGTCGGTACC ACG
55





CGTGGCGGATGT




11
0
+
CGCGAATGCC GCTTGGCGGGGGCTCCGAGGACGCCAAGGAGATCA ACG
56





CGTGGCGGATGT




11
35
−
CGCGAATGCC CTGCCACACGATACCGGCAAAGAAGCGATGCTGCA ACG
57





CGTGGCGGATGT




11
70
+
CGCGAATGCC CACGTGTACGAGAAGAAGGTGCGGCTGCTCCCCGC ACG
58





CGTGGCGGATGT




12
−35
−
CGCGAATGCC CTGCAGAGGTGGGCAGACGGGACAGTCATGAGCTT ACG
59





CGTGGCGGATGT




12
0
+
CGCGAATGCC CTCAGCCCACCCTTCAAGCCCCAGGTCACGTCGGA ACG
60





CGTGGCGGATGT




12
35
−
CGCGAATGCC CCGTGAACTCCTCATCAAAATACCTGGTGTCAGTC ACG
61





CGTGGCGGATGT




12
70
+
CGCGAATGCC CCCAGATGATCACCATCACACCACCTGACCAAGGT ACG
62





CGTGGCGGATGT




13
−35
−
CGCGAATGCC CTGTGGGTGTAGACAGCTCAGACCCCGGTGCCCCA ACG
63





CGTGGCGGATGT




13
0
+
CGCGAATGCC ATGACAGCATGGAGTGTGTGGACAGCGAGCGCAGG ACG
64





CGTGGCGGATGT




13
35
−
CGCGAATGCC CCGCTGGCCGAGTAGGAGAACTGGGGGAAGTGGGG ACG
65





CGTGGCGGATGT




13
70
+
CGCGAATGCC CACGGCCTGAGGCGGCGGTGGACTGCGCTGGACGA ACG
66





CGTGGCGGATGT

























































































































































































































Oligo Synthesis: For this experiment, the initial population of oligonucleotides 100 were synthesized individually in solution by Operon (Huntsville, Ala.). In the future, the oligonucleotides will be synthesized on an array then cleaved. For example, synthesized and cleaved oligonucleotides are commercially available (e.g., available from LC Sciences, Houston, Tex., Agilent also manufactures “Sure-print” oligo arrays, cleaves the oligos and delivers pmol quantities of single-strand reagent).
Annealing and Fill-in to Generate Double-Stranded Capture Probe Precursors:
As shown in FIG. 4 , Step A, the population of synthesized single-stranded oligonucleotide precursors 100 (free from substrate) were annealed with a common reverse primer oligonucleotide 150 and filled in with Klenow to form double-stranded capture probe precursor molecules 230 as shown in FIG. 4 , Step A. The common reverse primer oligo 150 was designed to hybridize to the region 204 for binding to a capture reagent on the synthesized oligos 100 , and includes the complement to the remaining portion of the region 204 , as well as the complement to the 3′ flanking region 220 . An advantage to the second strand fill in reaction with the reverse primer oligo 150 is the ability to change the 3′ flanking region 220 through the use of a reverse primer oligo 150 with different sequences in order to incorporate different restriction enzyme sites or other desired sequences in the 3′ flanking region 220 of the double-stranded capture probe precursor molecule 230 .
Design of the Second Strand Reverse Oligonucleotide 150 :
The following reverse primer oligonucleotides 150 were used in this Example:
Reverse primer #1: (Psi 1, Bsm1 3′ end) (SEQ ID NO: 67) 5′ GAGGTCGGCATTCTTATAATTGCTCGAAGGGGTCCACATC CGCCACGCGT 3′; Reverse primer #2: (HindIII, Bsm1 3′ end) (SEQ ID NO: 68) 5′GAGGTCGGCATTCAAGCTTAATTGCTCGAAGGGGTCCACA TCCGCCACGCGT 3′
Preparation of Oligo Pools and Annealing:

As shown in FIG. 4A , each oligonucleotide 100 in the pool of synthesized oligonucleotides (e.g., pool D, SEQ ID NO:3-66) was annealed to a common reverse primer 150 (SEQ ID NO:67 or SEQ ID NO:68) and filled-in with Klenow to generate a double-stranded oligonucleotide precursor 230 as follows:
Pooling of Oligonucleotides:
Each A, B, C, and D series oligos were resuspended to 100 μM, then each of the oligos in the A, B, C, and D series were pooled separately, to create an A series pool, a B series pool, a C series pool, and a D series pool at 100 μM, each pool containing a mixture of 64 different oligonucleotides. The DNA concentration of the oligo pool (100 μm of 70 to 80mer=2 μg/μl) was confirmed by agarose gel electrophoresis by diluting the pool 50-fold to 40 ng/μl, and loading 2.5 μl and 5 μl.
The common reverse primer 150 (#2: SEQ ID NO:68) for second strand synthesis was resuspended to 100 μM.
Annealing:
The following reagents were combined to give a 1 μM solution in each primer (40 ng/μl combined):
10 μl of 100 μM primer pool C or D (SEQ ID NO:3 to SEQ ID NO:66)
10 μl of 100 μM reverse primer #2 (SEQ ID NO:68)
100 μl New England Biolabs Buffer #4
880 μl H 2 0
1000 μl total
A 100 μl aliquot of the above mixture was heated to 95° C., then cooled down as shown below to room temperature:
95° C., 2 minutes
80° C., 1 minute
75° C., 1 minute
70° C., 1 minute
65° C., 1 minute
60° C., 1 minute
55° C., 1 minute
Room temperature, hold.
A 2.5 μl and 5.0 μl aliquot of the annealed mixture was checked on an agarose gel.
Fill-in Reaction:
The annealed mixture was then treated with Klenow to fill in both strands, thus generating a population of blunt ended, double-stranded precursor molecules 230 as shown in FIG. 4 , Step B, each having a target-specific region 202 and a common sequence region 204 for binding to a capture reagent. It will be understood by those of skill in the art that the double-stranded precursor oligo structure 230 may also be generated by synthesis of the top and bottom strands, followed by annealing of the single strands into a double-stranded structure having the desired “GG” nucleotide overhang on the first end and the desired “CC” nucleotide overhang on the second end of the molecule to facilitate head to tail concatemerization upon ligation.
Second strand synthesis of each of the annealed oligo mixtures “C” and D″ were carried out as follows:
100 μl annealed primer mixture, described above
1 μl 10 mM dNTPs
2 μl Klenow (3′ to 5′ exo-) 5000 units/ml (M0212S), New England Biolabs, MA
Incubated at 37° C. for 30 minutes, 75° C. for 20 minutes. A 3 μl or 6 μl aliquot of each pool was checked on an agarose gel.
Exemplary double-stranded capture probe precursors 230 are provided below based on the “D” series oligo for exon 1 (−35) (SEQ ID NO:3), provided in Table 1:
The top strand of the filled-in double stranded product (SEQ ID NO:3 annealed to Reverse primer #1 SEQ ID NO:67) is:





(SEQ ID NO: 69)

5′ CGCGAATGCC GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCT AC



GCGTGGCGGATGT GGACCCCTTCGAGCAATTATAAGAATGCCGACC



TC 3′

(3′ flanking region: Psi1/Bsm1)














The bottom strand of the filled-in double-stranded product (SEQ ID NO:3 annealed to Reverse Primer #1 SEQ ID NO:67) is:





(SEQ ID NO: 70)

5′GAGGTCGGCATTCTTATAATTGCTCGAAGGGGTCCACATCCGCCACG



CGT AGGAGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCGGCATTCG



CG -3′

(3′ flanking region: Psi1/Bsm1)














The top strand of the filled-in double-stranded product (SEQ ID NO:3 annealed to Reverse Primer #2 SEQ ID NO:68) is:





(SEQ ID NO: 71)

5′ CGCGAATGCC GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCT AC



GCGTGGCGGATGT GGACCCCTTCGAGCAATTAAGCTTGAATGCCGACC



TC-3′

(3′ flanking region: HindIII/Bsm1)














The bottom strand of the filled-in double stranded product shown is:
(SEQ ID NO: 72) 5′GAGGTCGGCATTCAAGCTTAATTGCTCGAAGGGGTCCACATCCGCCA CGCGT AGGAGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCGGCATTCG CG -3′ (3′ flanking region: HindIII/Bsm1)
Digestion with Bsm1 to Generate Ligation Substrates:

As shown in FIG. 4 , Step B, the filled-in double-stranded capture probe precursors 230 were then digested with Bsm1 to generate ligation substrates as follows:
2 μl of Bsm1 (10,000 units/ml, R0134S, New England Biolabs) was added to each of the 100 μl heat inactivated fill-in reactions and incubated at 65° C. for 1 hour. 3 μl and 6 μl of each pool was checked on an agarose gel. The Bsm1 digests were then purified over a QIAQUICK column (Qiagen), eluted and quantified by nanodrop.
Resulting exemplary double-stranded oligonucleotide structures after Bsm1 digestion, as illustrated in FIG. 4 , Step B:
The top strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:69/SEQ ID NO:70) is:





(SEQ ID NO: 73)

5′GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCT ACGCGTGGCGGA



TGT GGACCCCTTCGAGCAATTATAAGAATGCC 3′

(3′ flanking region: Psi1)












The bottom strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:69/SEQ ID NO:70) is:





(SEQ ID NO: 74)

5′CATTCTTATAATTGCTCGAAGGGGTCCACATCCGCCACGCGT AGGAG



CGCGTGAGCGTCGCGGGAGCCTCGGGCACCGG 3′

(3′ flanking region: Psi1).












The top strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:71/SEQ ID NO:72) is:





(SEQ ID NO: 75)

5′GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCT ACGCGTGGCGGA



TGT GGACCCCTTCGAGCAATTAAGCTTGAATGCC 3′

(3′ flanking region: HindIII)












The bottom strand of the Bsm1 digested double-stranded oligo (SEQ ID NO:71/SEQ ID NO:72) is:
(SEQ ID NO: 76) 5′CATTCAAGCTTAATTGCTCGAAGGGGTCCACATCCGCCACGCGT AGG AGCGCGTGAGCGTCGCGGGAGCCTCGGGCACCGG 3′ (3′ flanking region: HindIII)
Ligation of Bsm1 Digested Precursors to Form Head-to-Tail Concatemers:

As shown in FIG. 4 , Step C, the Bsm-1 digested products 230 were then used as ligation substrates to generate a series of head-to-tail concatemers 240 for use as amplification templates.
Ligation was carried out as follows:
The following reagents were combined for each pool “C” and “D”:
50 μl 2× quick ligase buffer (New England Biolabs)
11 μl of 48 ng/μl Bsm1 digested pool C or D
34 μl H 2 O
5 μl ligase (New England Biolabs)
100 μl total
Incubated at room temperature for ≧10 minutes. A no DNA control (no template) was also prepared.
As illustrated in FIG. 4 , Step C, it is noted that the ligation reaction naturally drives towards the formation of circularized templates due to the fact that in very dilute solutions of free ends, the probability of self-ligation (circularization) becomes higher than the probability of finding a separate free end.
Amplification of Head-to-Tail Concatemers:
As shown in FIG. 4 , Step D, circularized concatenated DNA was then amplified by greater than 1000 fold (i.e., 10,000 fold to 20,000 fold) to form amplified concatemers 260 by using a random amplification primer 250 with the strand displacement polymerase technology found in the TEMPLIPHI isothermal amplification kit (GE Life Sciences, Piscataway, N.J.).
The random amplification primer 250 used in this Example was a random 7-mer amplification primer with an additional two nitroindole residues at the 5′ end and a phosphorothioate linkage at the 3′ end: 5′[nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77, wherein the “N” at positions 1-7 may be A, G, C or T), as described by Lage et al., Genome Res 13:294-307 (2003), incorporated herein by reference.
Isothermal Amplification:
Four reaction mixtures of “C” pool and “D” pool ligations were prepared as follows, along with 2 reaction mixtures of a “no template” control:
25 μl of 100 μM amplification primer [5-nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77)
10 μl ligated template “C” or “D” pool (or no template control)
5 μl 10× phi29 buffer (New England Biolabs)
10 μl H 2 0
50 μl total
The above reagents were mixed and incubated at 95° C. for 3 minutes, then cooled to room temperature, then 50 μl of the following enzyme premix was added:
Enzyme Premix:
25 μl of 100 μM amplification primer (SEQ ID NO:77)
10 μl H 2 0
5 μl 10× phi29 buffer (NEB)
4 μl 10 mM dNTPs
2 μl 100 mM DTT
2 μl 10 mg/ml BSA (NEB)
2.5 μl phi29 polymerase (NEB)
50 μl total volume of enzyme premix
The 50 μl annealed mixture was combined with the 50 μl enzyme premix, then incubated at 30° C. for 12 hours. The polymerase was inactivated by incubation at 65° C. for 10 minutes, then cooled to 4° C. The mixture was briefly centrifuged to pellet the protein and the supernatant was transferred to a fresh tube.
The isothermal amplification reactions were then ethanol precipitated by combining the four “C” reactions and the four “D” reactions (separately), then adding 600 μl TEzero, split into two tubes of 500 μl each, then adding 120 μl 3M NaOAc at pH 5.2 to each tube, then 1200 μl ethanol. The reactions were centrifuged for 10 minutes at 12K RPM and the pellets were resuspended in 880 μl TEzero. The amount of DNA recovered was quantitated. The yield was determined to be 65 to 79 ng/μl for all four tubes, therefore, each 100 μl isothermal amplification reaction produced 28.5 to 30 μg of DNA, which was an unexpectedly high yield. It is noted that the isothermal amplification reaction carried out with 50 μM of the random amplification primer [5-nitroindole]2-[N]6-(phosphothioate)-N (SEQ ID NO:77) in combination with 400 nM dNTPs and DTT as described above provided reaction conditions that yielded a significantly higher amount of amplification product than was obtained from an amplification reaction with the same templates using the reagents from a commercially available kit (GE Healthcare Life Sciences).
It is noted that an initial attempt was made to simply ligate dsDNA blunt-end oligonucleotide probes together followed by amplification (Series “B” pool). However, it was determined that this initial approach was not suitable for uniformly amplifying a population of probes for solution-based capture because the oligonucleotides were ligated in random head to tail, head to head and tail to tail orientation. In the head to head and tail to tail orientations some common tail sequences hybridized together, thereby creating a snap-back stem in ssDNA. These ssDNA stem regions were poisonous to polymerases, resulting in under-representation of amplified products.
Digestion of Amplified Concatenated Strands into Monomer Double-Stranded Capture Probe Precursors:
As shown in FIG. 4 , Step E, the amplified concatenated strands 260 were then cleaved into monomer double-stranded capture precursors 270 with Bsm1 and 5′ terminal phosphates were removed with Antarctic Phosphatase, as follows:
Digestion with Bsm1 after Isothermal Amplification:
55 μg from each amplification reaction was digested with 20 μl Bsm1 in a total volume of 1 ml in 1×NEB Buffer #4 buffer at 65° C. for two hours. After the two hour digestion, an aliquot of each digest was checked on an agarose gel. The majority of the “C” and “D” digested pool showed the expected 90 bp product, with a small amount of 130 bp product.
Phosphatase Treatment:
110 μl 10× Antarctic Phosphatase buffer (NEB) was added to the Bsm1 digested pool C and D. 50 μl Antarctic phosphatase was added (NEB), and incubated for one hour at 37° C. then 65° C. for 15 minutes. An aliquot of 10 μl was taken out of each sample, then the samples were ethanol precipitated.
After ethanol precipitation, each sample was split into four tubes of 250 μl each to which was added: 250 μl TEzero, 120 μl of 3M NaOAc pH5.2, 2 μl glycol and mixed. 1200 μl ethanol was added per tube, mixed, precipitated and centrifuged at 12K RPM, 10 minutes.
Digestion With Psi1 or HindIII to Liberate the Precise 3′ Ends of the Capture Probes:
As shown in FIG. 4 , Step F, monomeric capture precursors and the precise 3′ ends of capture probes were then liberated by digestion with Psi1 or HindIII, which also leaves 5′ terminal phosphates on the complementary strands.
After the phosphatase treatment as described above, the pellets were then resuspended in 960 μl of 1×NEB #4 buffer and 40 μl PsiI, then digested for 2 hours at 37° C. The digests were then ethanol precipitated.
Selective Removal of the Complementary Strand of the Monomer Capture Probe Precursors to Produce a Population of Single-Stranded Capture Probes for Solution Based Capture:
As shown in FIG. 4 , Step G, a population of single-strand capture probes 200 (i.e., a library), each probe 200 containing a distinct target specific region 202 and a common capture reagent binding region 204 , were generated by digesting the double-stranded monomer capture probe precursors 270 with Lambda exonuclease. Lambda exonuclease specifically degrades dsDNA by attacking at 5′ phosphate sites and selectively digests away the non-capture complementary strand, thereby converting the dsDNA into ssDNA suitable for use as capture probes.
Lambda Exonuclease Digestion:
An enzyme titration was first run with lambda exonuclease starting at a concentration of 1 μl/10 μl and diluted down in 2-fold steps. Each reaction contained 10 μg substrate (digested with Bsm1, alkaline phosphatase and Psi1) in 100 μl and were digested at 37° C. for 10 minutes, 75° C. for 10 minutes, then cooled to 4° C. The reactions were run on an agarose gel and the conditions of 10 μg substrate in 100 μl with 5 μl exonuclease for 10 minutes at 37° C. was used for subsequent digestions of the pool C and pool D substrate.
Scaled Up Reaction:
For capture series “C”, 90 μl of 89 ng/μl dsDNA precursor was digested with 5 μl lambda exonuclease in 100 μl 1× lambda exonuclease buffer (NEB).
For capture series “D”, 100 μl of 208 ng/μl dsDNA precursor was digested with 5 μl lambda exonuclease in 200 μl 1× lambda exonuclease buffer (NEB).
The following is an exemplary structure of a final single-stranded capture probe 200 resulting from the capture probe precursor SEQ ID NO:69/70 or SEQ ID NO:71/72 after processing with lambda exonuclease:





(SEQ ID NO: 78)

5′GGTGCCCGAGGCTCCCGCGACGCTCACGCGCTCCT ACGCGTGGCGGA



TGTGGACCCCTTCGAGCAATTA 3′ 3′











The capture probe SEQ ID NO:78 comprises a 5′ region 202 that hybridizes to the −35 exon 1 of the AKT gene, and a 3′ region 204 (underlined) that hybridizes to a universal biotinylated oligonucleotide 300 .
The universal capture hybridizing region 204 of capture probe SEQ ID NO:78 is:
(SEQ ID NO: 80) 5′ ACGCGTGGCGGATGTGGACCCCTTCGAGCAATTA 3′
Discussion:

The isothermal amplification method described in this Example converted 2.5 ng (0.1 μmol) of starting material into 50 μg (2000 μmol) of raw, unprocessed double-stranded material, which is a 20,000-fold level of amplification. In comparison, the Agilent custom library array platform provides 250 ng (10 μmol) of cleaved oligonucleotide. Processing of the double-stranded DNA precursor capture probe 230 to single stranded capture probes 200 resulted in approximately 15 μg (1200 μmol) of single-stranded capture probe 200 , which is a 60% yield from the starting amplified unprocessed double-stranded material 230 . In addition to high yield of amplified products, the use of concatemers 240 as templates for amplification provides an equal distribution of amplified monomer products 270 , resulting in an equal distribution of processed capture probes 200 . The lambda exonuclease digested material (ssDNA) 200 was successfully used as a capture probe library for solution based capture of the AKT exons 1-13, as described in Example 2.
Example 2
This Example describes solution-based capture using a pool of capture probes 200 generated as described in Example 1, each capture probe 200 comprising a target specific region 202 specific for binding to one of the 13 exons of AKT (NM — 005163) and a common region 204 that hybridizes to a universal biotinylated adaptor oligo 300 .
Rationale:
As shown in FIG. 2 , target gene enrichment of a genomic library may be achieved by indirect capture using a pool of chimeric capture ssDNA probes 200 with a first region 202 that hybridizes to a target nucleic acid sequence 10 and a second region 204 that hybridizes to a universal biotinylated oligo 300 , mixing the chimeric oligo 200 , the universal biotinylated oligo 300 and the sample containing the target nucleic acid sequence 10 under hybridizing conditions to form a tri-molecular complex (i.e., 10 / 200 / 300 ), and using magnetic beads 400 coated with streptavidin 410 to bind to the biotinylated region 310 of the universal oligo 300 and pull out the target sequences 10 bound in the complex to the chimeric capture probes 200 , using a magnet 500 .
While indirect capture is described in this Example, it will be understood by those of skill in the art that solution based capture may also be accomplished through the use of ssDNA probes 200 are directly labeled. For example, the probes 200 could be directly labeled by adding a biotin, deoxygenin, fluorescein, and the like (through the use of commercially available kits), followed by the use of antibody coated beads for purification.
Methods:
ssDNA capture probes were generated as described in Example 1. For capture series “C” and “D,” the concentration of capture probe after the lambda exonuclease digestion was approximately 40 ng/μl to 50 ng/μl.
The following universal 5′ biotinylated oligo (capture reagent 300 ) was used in this Example:







(SEQ ID NO: 81)

5′ [BioTEG] TAATTGCTCGAAGGGGTCCACATCCGCCACGCGT 3′












As described in Example 1, a library of 64 ssDNA chimeric capture oligos 200 were generated that each target one of the 13 exons of AKT1 that each have a first 5′ region 202 with the identical sequence to the oligos shown above in TABLE 1, and a second 3′ region 204 consisting of the following additional sequence that hybridizes to the universal biotinylated oligo 300 :
(SEQ ID NO: 80) 5′ ACGCGTGGCGGATGTGGACCCCTTCGAGCAATTA 3′.
Capture Mixture:

A master mix was prepared by combining 62.5 μl of 80 ng/μl of a genomic DNA library containing an average insert size of 100 bp flanked by a first and second primer binding site, 10 μl of 1 μM universal biotinylated oligo (SEQ ID NO:81), 125 μl 2× binding buffer (20 mM Tris pH 7.6, 0.2 mM EDTA, 2M NaCl).
The master mix was then combined (separately) with the following:
10 μl of 1 μM Maxwell 139 AKT1 set, a set of 28 directly synthesized capture oligonucleotides specific for AKT exons 1-13 (low density coverage).
10 μl of 1 μM “A” series high density AKT1 control set (64 oligo pool) that was directly synthesized (not amplified). Each capture probe was synthesized to contain only the target specific portion 202 and the universal oligo hybridizing portion 204 , without the flanking sequences. For example, the probe for AKT exon 1 (−35) was identical to the final processed amplified probe for AKT exon 1 (−35) (SEQ ID NO:78).
10 μl of processed “C” Series Capture probes (in duplicate): a set of 64 AKT capture probes generated using the amplification method described in Example 1.
10 μl of processed “D” Series Capture probes: a set of 64 AKT capture probes generated using the amplification method described in Example 1.
20 μl of processed “D” Series Capture probes, as described for Sample #4 above.
Each reaction was brought to a total volume of 250 μl, mixed, and annealed as follows:
95° C. for 5 minutes
80° C. for 15 minutes
75° C. for 15 minutes
70° C. for 15 minutes
65° C. for 15 minutes
60° C. for 15 minutes
55° C. for 15 minutes
Room Temperature.
Capture Reagents:
Washed streptavidin-coated magnetic beads were prepared by combining 66 μl beads (MyOne streptavidin-coated beads, InVitrogen) 500 μl 2× binding buffer and 440 μl water.
Capture:
Each of the annealed 250 μl mixtures were combined with 10 μl of washed beads in a total volume of 1 ml (10 mM Tris, pH 7.6, 0.1 mM EDTA, 1 M NaCl, 0.1% Triton X100) and incubated with mixing for 15 minutes. The beads were then washed four times, 5 minutes each, with 1 ml of TEzero wash buffer (10 mM Tris pH 7.6, 0.1 mM EDTA) containing 25% formamide.
Elution:
The DNA bound to the beads was eluted with two 25 μl aliquots of water by incubation at 95° C. for 1 minute, pulling over the beads, and removing the eluate, for a total eluate volume of 50 μl
Amplification of Eluate:
PCR Reaction Mixture (Each Sample Performed in Duplicate)
10 μl template (eluate from enriched fragment library)
30 μl H 2 O
20 μl 5×PCR buffer (supplied by manufacturer with the EXPAND plus ® kit, Roche)
10 μl 25 mM MgCl 2







10 μl Forward PCR primer


(5′-AATGATACGGCGACCACCGA-3′ (SEQ ID NO: 82))




10 μl Reverse PCR primer


(5′-CAAGCAGAAGACGGCATACG-3′ (SEQ ID NO: 83))












5 μl 10 mM dNTPs
5 μl DMSO
1 μl Expand PLUS ® polymerase (Roche)
100 μl total volume
PCR Cycling Conditions:
1 Cycle:
95° C. for 2 minutes
10 Cycles:
95° C. for 30 sec
60° C. for 30 sec
72° C. for 1 minute
10 Cycles:
95° C. for 30 sec
60° C. for 30 sec
72° C. for 1 minute plus 10 sec/cycle
1 Cycle:
72° C. for 5 minutes
4° C. hold
The PCR products were purified over a QIAQUICK column, quantified and diluted to 1 ng/μl for subsequent quantitative PCR (qPCR) (Taqman) analysis.
Quantitative PCR Analysis:
The PCR products generated as described above (1 ng/μl), no template control and genomic DNA control (10 ng/μl) were used as templates in Taqman assays directed against coding exons 1-13 of AKT1. Negative controls ANKHD, PIK3CA and TP53 were also included in this assay, which should not be captured as target sequences with the AKT specific probe pools.
Results:
The results of the qPCR analysis were analyzed in two ways. First, the fold-enrichment for each solution-based capture over genomic DNA was calculated, as shown in TABLE 2.





TABLE 2



Fold Enrichment relative to genomic DNA of solution-

based capture using various capture probe pools












Series
Series



Targeted
Maxwell
Series
C
C
Series
Series

AKT
139
A
(set #1)
(set #2)
D
D

exon
(10 μl)
(10 μl)
(10 μl)
(10 μl)
(10 μl)
(20 μl)











exon 1
1970
1158
3471
3376
3802
6370

exon 2
1338
931
2790
2895
3662
6450

exon 3
1131
989
2480
2568
3452
6044

exon 4
1579
1072
2508
2507
4189
7122

exon 5
1460
1189
3408
3840
5184
8878

exon 6
1641
1002
3083
3461
4048
5586

exon 7
1435
1048
3578
3660
4642
6167

exon 8
1331
1112
2408
2212
3780
7418

exon 9
1242
1194
3495
3817
4746
8526

exon 10
1528
1227
3146
3176
4675
9464

exon 11
1314
1103
2592
2638
3741
6904

exon 12
1478
960
1964
2511
3739
6862

exon 13
1662
1107
754
648
4236
6504

ANKHD
1
1
1
0
0
1

PIK3CA
0
0
0
0
0
0

TP53
0
1
1
0
0
1











































Second, the standard deviation of the exon-to-exon capture efficiency was calculated as a percent of the overall fold-enrichment, as shown below in TABLE 3. This latter number provides a measure of the uniformity of each capture reaction.





TABLE 3



Standard deviation of the exon-to-exon capture efficiency












Series
Series




Max
Series
C
C
Series
Series


139
A
(set #1)
(set #2)
D
D


(10 μl)
(10 μl)
(10 μl)
(10 μl)
(10 μl)
(20 μl)












Average fold
1470
1084
2745
2870
4146
7100

enrichment

for AKT

exons 1-13

standard
216
94
782
863
523
1175

deviation of

exon-to-exon

capture

efficiency

Percent
15%
9%
28%
30%
13%
17%

deviation,

expressed as

a percentage

of the fold-

enrichment









































FIG. 6 graphically illustrates the fold of enrichment of the target AKT exons 1 to 13 (shown on the x-axis) relative to genomic DNA of solution-based capture using 100 of each of the following probe pools: the “Maxwell 139” capture probe pool (low density, directly synthesized oligonucleotides), the “Series A” capture probe pool (high density, directly synthesized oligonucleotides) and the “Series D” capture probe pool (high density, generated via amplification of head-to-tail concatemers). As shown in FIG. 6 , the fold enrichment using the Series D capture probe pool was at least as good as the fold enrichment using the Series A capture probe pool.
Discussion:
The “A” series oligos were directly synthesized 69 mers (non-amplified) containing the identical sequence as the probe sequences generated after processing amplified sequences. The “C” series oligos were initially synthesized as 79mers, annealed to a reverse primer, filled-in, digested with Bsm1, ligated into concatemers, amplified, digested back to monomers, and lambda-exo treated to generate ssDNA probes. The “D” series oligos were initially synthesized as 60mers (less overlap with reverse primer than the “C” series), annealed to a reverse primer, filled-in, digested with Bsm1, ligated into concatemers, amplified, digested back to monomers and lambda-exo treated to generate ssDNA probes. It is important to note that the “D” series oligos are the least expensive to generate due to the fact that they can be made on a 50 nmole synthesis scale due to their smaller size. As described above in Example 1, the amplification of the capture probe pool “C” or “D” was observed to be in the range of 10,000 to 20,000 fold amplification, thereby facilitating the cost-effective use of solution based capture for target enrichment in a variety of applications.
It is noted that the absolute magnitude of the fold-enrichment shown in TABLE 2 may be exaggerated because the critical stoichiometry between the capture oligonucleotides and the common, biotinylated adaptor capture reagent oligonucleotide was not optimized in this experiment. However, the key metric is that the standard deviation of exon-to-exon capture efficiency, expressed as a percentage of the fold-enrichment, as shown in TABLE 3 and FIG. 6 , was essentially identical for the synthetically generated capture probes (Series A=9%) versus the capture probes generated using the amplification methods described in Example 1 (Series D=13%).
Agarose gel analysis of the capture probes generated by the amplification methods described herein showed that they formed bimolecular complexes during solution based capture that were indistinguishable from the biomolecular complexes formed with directly synthesized capture probes. Importantly, as demonstrated in TABLES 2 and 3 and FIG. 6 , the solution based capture of the 13 exons of the AKT gene with the capture reagent generated by amplification worked at least as well as control reactions performed with directly synthesized capture probes. This conclusion is based on the metrics of fold-enrichment and on the standard deviation of exon-to-exon enrichment as a percentage of the overall fold-enrichment.
Therefore, this Example demonstrates that the capture probes generated from amplified head-tail concatemers may be successfully used for solution based capture, and provide an advantage in targeted resequencing by reducing the cost of resequencing while increasing the feasibility of profiling applications that are dependent on complex oligonucleotide libraries.
Example 3
This Example describes a method for designing and uniformly amplifying a library of target capture probes designed to capture the entire collection of exons that include protein coding regions from a human genomic DNA library.
Rationale:
As described in Examples 1 and 2, a method for uniformly amplifying a library of synthesized capture oligonucleotides for use in solution based capture methods can be applied to capture all the exons of a gene, such as AKT. This Example demonstrates that the methods described herein can be applied on a very large scale, in order to generate a library of capture probes that capture the entire collection of genomic segments that include all protein coding regions from a human genomic library.
Methods:
Design of Capture Probe Oligonucleotide Precursors for Amplification and Processing into a Library of Capture Probes:
As shown in FIG. 4 , step A, the general structure of the double-stranded capture probe precursor includes a 5′ flanking region 210 comprising a first restriction enzyme site for creating a first nucleotide overhang for ligation (e.g., Bsm1), a capture probe region 200 comprising a target specific hybridizing region 202 and a universal capture oligo hybridizing region 204 , and a 3′ flanking region 220 comprising a second restriction enzyme site (e.g., Bsm1) for creating a second nucleotide overhang for ligation, and further comprising a third restriction enzyme site (e.g., Psi1 or HindIII), for precisely cleaving off the 3′ flanking region.
Design of the Target-Specific Region 202 of the Capture Probe 200 :
In this Example, a capture probe library 200 comprising 1,148,286 distinct target-specific regions 202 was generated for high density solution based capture of the entire collection of genomic segments that include protein coding regions (exons) for 25,341 annotated human genes.
The overall design principles for designing a capture probe library 200 comprising target-specific regions 202 for capture of all the exons of 25,341 human genes were as follows. Each target-specific region 202 was 35 nucleotides in length. The target-specific region was designed such that each target exon had at least 4 or more probes (high density), and the capture probes alternated in strand orientation and were oriented in a head to tail arrangement, with oligo probes alternating with respect to hybridizing to the coding or non-coding strand of the target exon.
In this Example, the term “candidate oligonucleotide” refers to a 35mer nucleotide sequence that was analyzed for potential use as target-specific region in a capture probe, to determine whether the candidate oligonucleotide sequence met the desired criteria, as described below. The 35mer nucleotide sequences that met all of the design criteria outlined herein were chosen as the set of target-specific regions 202 and were synthesized on a microarray along with flanking sequences to generate an oligonucleotide library having 1,148,286 distinct target-specific regions.
Step 1: Obtain Input Sequence:
In order to design the target-specific sequences of the library of capture probes, the genes and transcripts of interest were first identified. In this Example, the entire list of human high quality mRNA transcripts provided in the publicly accessible NCBI database “RefSeq NM transcripts” was selected as input sequence, which was a total of 25,341 human annotated mRNA transcripts. The protein coding exons in the 25,341 input human mRNA transcripts were then identified using the publicly accessible “UCSC Genome Browser” database. The UCSC Genome Browser is developed and maintained by the Genome Bioinformatics Group, a cross-departmental team within the Center for Biomolecular Science and Engineering at the University of California Santa Cruz. Once the protein coding exons were identified, the genomic sequences of interest plus 100 nucleotides of adjacent intronic sequences on either side of the exons were extracted. Overlapping regions were then identified and removed from the list of sequences by using genomic coordinates. For overlapping regions that were identified, the sequence that was retained was based on the 5′ most and 3′ most genomic coordinates between all the pairs of exons in the overlapping region.
Step 2: Upfront Sequence Classification:
The list of sequences generated as described in Step 1 was then searched using the software program “repeatmasker” to identify, but not mask, the repeat and low complexity elements.
Step 3: Determination of Uniqueness Score of Candidate Oligonucleotide Probe Sequences:
The uniqueness of all 35mer sequences in the sequenced human genome relative to the human genome was determined as follows. First, the “UCSC Genome Browser” database was used to extract every 35mer sequence from the sequenced human genome. Second, the software algorithm “Burrows-Wheeler Alignment” (hereinafter referred to as “BWA”) was used to align (i.e., blast) these 35mer sequences against the entire sequenced human genome. BWA is a fast light-weighted tool that aligns short sequences to a sequence database, such as the human reference genome. For each 35mer candidate oligo sequence, BWA returned an alignment score that measures the confidence in the identified location, as described in Li, H., et al., Genome Res. 18(11):1851-8 (2008), incorporated herein by reference. The alignment score provided by BWA is a −log 10 p-value, ranging from 37 (unique in the genome) to 0 (multiple perfect matches). Intermediate values reflect alignments that are similar but not 100% (e.g., with one nucleotide mismatch).
Step 4: Final Selection of the Target-Specific Regions 202 for Synthesis to Generate a Library of Capture Probes 200 for Total Exon Capture of the Human Genome:
The final selection of the 1,148,286 distinct target-specific regions for synthesis in order to generate a library of capture probes 200 that hybridizes to all the exons of 25,341 annotated genes was carried out as follows.
For each target exon at least 140 nucleotides or longer, analysis for candidate oligos began at the 5′ edge of the exon. For target exons 140 nucleotides or shorter, the analysis began in the 5′ intron such that the candidate oligos were equally spaced at the exon center.
From the starting position, each candidate oligo location was then “jittered” by shifting the region of analysis by +/−4 nucleotides as follows. The nucleotide sequence of the candidate oligo closest to the desired location was first examined, and the examination of candidate oligos was carried out by shifting the region of analysis in the order: +1, −1, +2, −2, +3, −3, +4, −4. The +/−4 nucleotide range was chosen because a wider range (more “jitter”) may allow oligo dimers to form with adjacent oligos, which would be undesirable.
The best candidate oligos from each “jittered” position were then selected based on the following criteria. First, the BWA alignment score (uniqueness) of the candidate 35mer capture oligo sequences was maximized relative to the entire genome (wherein 37=unique and 0=multiple perfect matches). Second, among the candidate 35mer capture oligo sequences with the same BWA alignment score, the number of repeat/low-complexity nucleotides contained in the 35mer sequence was minimized.
The nucleic acid sequences of the 1,148,286 distinct target-specific 35mer regions were output as a text file to an oligonucleotide synthesis platform and synthesized.
In this Example, the target-specific regions were flanked by a 3′ flanking region (SEQ ID NO:1) for annealing to a common reverse primer for second strand synthesis, and by a 5′ flanking region (SEQ ID NO:2) which provides at least one processing site for ligation and amplification, resulting in a structure corresponding to the single-stranded capture probe precursor 100 as illustrated in FIG. 4 , Step A, and described in Example 1. The processing of the library of capture probe precursors 100 to double-stranded precursors 230 , processing of the 5′ and 3′ flanking regions to generate ligation substrates, ligation into head-to-tail concatemers, amplification and processing of the amplified head-to-tail concatemers to form monomers, and selective removal of the non-capture complement strand of the monomers to form a library of single-stranded capture probes, is carried out as described in Example 1.
TABLE 4 below provides the sequence information and scores from the selection criteria described above for a representative, randomly chosen subset of 2170 capture probe precursors from the total library of 1,148,286 capture probe precursors that were synthesized.
In particular, TABLE 4 provides the following information: Column 1: gene name; Column 2: Genbank transcript reference number; Column 3: target exon number; Column 4: the chromosome of the target exon; Column 5: the gene strand; Column 6: the distance of the capture probe from the 5′ edge of the target exon; Column 7: the strand of the capture probe; Column 8: the number of repeat nucleotides; Column 9: the BWA alignment score (wherein 37=unique and 0=multiple perfect matches); Column 10: the number of hits from the BWA alignment (1=unique); Column 11: the number of hits from BWA mismatch (0=no mismatch; 1=mismatch); Column 12: the sequence of the single-stranded precursors, each including the common 5′ flanking region (SEQ ID NO:2) and common 3′ flanking region (SEQ ID NO:1); and Column 13: the SEQ ID NO: from SEQ ID NO:84 to SEQ ID NO:2253.
As shown in TABLE 4, Column 9, a score of “1” for the “number of hits from the BWA analysis corresponds to a uniqueness score of a perfect 37, in which capture oligo sequence only recognizes the intended target.





TABLE 4



Representative subset (2170) of a total of 1,148,286 Capture Probes Designed for Exon Capture of 25,341 Human

Genes




















Distance












Gene
from exon 5
Oligo
# repeat
BWA

# BWA

SEQ ID

Gene
Transcript
Exon
Chromosome
strand
edge
strand
nucleotides
score
BWA hit
mismatch
Sequence with add-ons
NO:

















KSR2
NM_173598
15
12
−
−35
+
0
37
1
0
CGCGAATGCCCCTCTGTGTAACAGGCTGTTCTCT
84












TCTCTCTGTAGACGCGTGGCGGATGT


KSR2
NM_173598
15
12
−
0
−
0
37
1
0
CGCGAATGCCGATCTGCGGGGACCAGCGTGGCA
85












CTGACAGTGTGTACGCGTGGCGGATGT


KSR2
NM_173598
15
12
−
35
+
0
37
1
0
CGCGAATGCCCCTCGCAGAGATCTCGGCAACTC
86












CATCAAGCACAGACGCGTGGCGGATGT


KSR2
NM_173598
15
12
−
70
−
0
37
1
0
CGCGAATGCCAAGTAGGCACCAGTGCACACAGG
87












AATCCAGCCTACACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
0
+
0
37
1
0
CGCGAATGCCTCCTGGCCCAGCTGCTGCCGCTC
88












CTGCACGGCAATACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
35
−
0
37
1
0
CGCGAATGCCTGGAACTCCCGGATGATGACCTT
89












GCTCCCGTTCACACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
70
+
0
37
1
0
CGCGAATGCCGGAGCACTGCCGCCGGGGACTGC
90












TCAGCAACCACAACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
105
−
0
37
1
0
CGCGAATGCCCAGGTAGGTGGTGGAGGGGCTCC
91












GCGGGCTGCCGGACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
140
+
0
37
1
0
CGCGAATGCCCACACCCCCACCCCCAGCGAGGA
92












TGCCGCCATCCCACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
175
−
0
37
1
0
CGCGAATGCCTCTCGGAAATGAGCCGCTTGAGC
93












CGGGACTTAGAGACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
210
+
0
37
1
0
CGCGAATGCCACTCAGTGTATGAGAAGCGGCCT
94












GACTTCAGGATGACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
245
−
0
37
1
0
CGCGAATGCCAAGCTCTGTAGCACCTGCGGGTG
95












CACGTACCAGCAACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
280
+
0
37
1
0
CGCGAATGCCCCAGCAGGAGCACCTGCCCGTGC
96












CGTGCCAGTGGAACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
315
−
0
37
1
0
CGCGAATGCCCTCTTTGGGGGCCGAGGGCACCG
97












ATGTCACATAGCACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
350
+
0
37
1
0
CGCGAATGCCGACAGTGGCAGCGTCCCCTCCAC
98












GGGGCCCAGCCAACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
385
−
0
37
1
0
CGCGAATGCCCCGCTGACTTCCTCTTCAGCGAG
99












ATGGGAGTGCCCACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
420
+
0
37
1
0
CGCGAATGCCGCAGCATGTGCATCACCCATTC
100












ATGAAGAAGCGCACGCGTGGCGGATGT


CHAF1A
NM_005483
13
19
+
455
−
0
37
1
0
CGCGAATGCCACCTGCCCACCCCACCTCACCTG
101












GCCGTCGTGCCTACGCGTGGCGGATGT


RBBP8
NM_002894
15
18
+
0
+
0
37
1
0
CGCGAATGCCATGAAGAAAGAAAAATGAATGA
102












TAGCTTGGAAGATACGCGTGGCGGATGT


RBBP8
NM_002894
15
18
+
35
−
0
37
1
0
CGCGAATGCCGATTCATACTCTTCATGTGTTGTC
103












CGATCAAACATACGCGTGGCGGATGT


RBBP8
NM_002894
15
18
+
70
+
0
37
1
0
CGCGAATGCCCTGTTTGGCAGACAGTTTCTCCCA
104












AGCAGCAGATGACGCGTGGCGGATGT


RBBP8
NM_002894
15
18
+
105
−
0
37
1
0
CGCGAATGCCTAGTTTCTTTGTGGCAGTAGACA
105












ATTCCTCCTCTTACGCGTGGCGGATGT


RBBP8
NM_002894
15
18
+
140
+
0
37
1
0
CGCGAATGCCCACAGTAAGATTTTTTTCTGTTTA
106












ATTATGGCTTCACGCGTGGCGGATGT


RBBP8
NM_002894
17
18
+
−22
+
0
37
1
0
CGCGAATGCCATTTAATTCATTTTTCCCCCAGAG
107












AGACTAGCTTGACGCGTGGCGGATGT


RBBP8
NM_002894
17
18
+
13
−
0
37
1
0
CGCGAATGCCTTTTTCCGAACCACCTCAATATGA
108












GGAAAATTTTGACGCGTGGCGGATGT


RBBP8
NM_002894
17
18
+
48
+
0
37
1
0
CGCGAATGCCAGAGGAGAGAAGAAAACTGCTT
109












GGGCACACGTGTAACGCGTGGCGGATGT


RBBP8
NM_002894
17
18
+
83
−
0
37
1
0
CGCGAATGCCAGTATCTACATTAGTACTTACAA
110












TTTCACATTCCTACGCGTGGCGGATGT


PDGFRA
NM_006206
8
4
+
−12
+
0
37
1
0
CGCGAATGCCTTTTTTTAAAAGGTATCGAAGCA
111












AATTAAAGCTGAACGCGTGGCGGATGT


PDGFRA
NM_006206
8
4
+
23
−
0
37
1
0
CGCGAATGCCAGTATAATGGCCACTGTCTTCTTC
112












CTTAGCACGGAACGCGTGGCGGATGT


PDGFRA
NM_006206
8
4
+
58
+
0
37
1
0
CGCGAATGCCATTGTAGCTCAAAATGAAGATGC
113












TGTGAAGAGCTAACGCGTGGCGGATGT


PDGFRA
NM_006206
8
4
+
93
−
0
37
1
0
CGCGAATGCCCCCTTTACATACCTTGAGTTAACA
114












GTTCAAAAGTAACGCGTGGCGGATGT


RB1
NM_000321
17
13
+
0
+
0
37
1
0
CGCGAATGCCGAAGTACATCTCAGAATCTTGAT
115












TCTGGAACAGATACGCGTGGCGGATGT


RB1
NM_000321
17
13
+
35
−
0
37
1
0
CGCGAATGCCAAATTAAGCACATTCAGAATCCA
116












TGGGAAAGACAAACGCGTGGCGGATGT


RB1
NM_000321
17
13
+
70
+
0
37
1
0
CGCGAATGCCAAAAGCCTTTGATTTTTACAAAG
117












TGATCGAAAGTTACGCGTGGCGGATGT


RB1
NM_000321
17
13
+
105
−
0
37
1
0
CGCGAATGCCCATTTCTCTTGTCAAGTTGCCTTC
118












TGCTTTGATAAACGCGTGGCGGATGT


RB1
NM_000321
17
13
+
140
+
0
37
1
0
CGCGAATGCCATAAAACATTTAGAACGATGTGA
119












ACATCGAATCATACGCGTGGCGGATGT


RB1
NM_000321
17
13
+
175
−
0
37
1
0
CGCGAATGCCTTTAGCTACTTACTGAGAGCCAT
120












GCAAGGGATTCCACGCGTGGCGGATGT


EPHA3
NM_182644
7
3
+
0
+
0
37
1
0
CGCGAATGCCCAGGAACAAGAAACAAGTTATAC
121












CATTCTGAGGGCACGCGTGGCGGATGT


EPHA3
NM_182644
7
3
+
35
−
0
37
1
0
CGCGAATGCCGCTTGAGGCTACTGATGGTAACA
122












TTTGTGCCTCTTACGCGTGGCGGATGT


EPHA3
NM_182644
7
3
+
70
+
0
37
1
0
CGCGAATGCCCTGACACTATATACGTATTCCAA
123












ATCCGAGCCCGAACGCGTGGCGGATGT


EPHA3
NM_182644
7
3
+
105
−
0
37
1
0
CGCGAATGCCAACTTGCGGCTGTTCGTCCCATAT
124












CCAGCGGCTGTACGCGTGGCGGATGT


EPHA3
NM_182644
7
3
+
140
+
0
37
1
0
CGCGAATGCCTGAGTTTGAAACTAGTCCAGACT
125












GTATGTATTATTACGCGTGGCGGATGT


EPHA3
NM_182644
7
3
+
175
−
0
37
1
0
CGCGAATGCCGCAAGATCCCTGCCCCCTCCTCT
126












AGACTGCATTGAACGCGTGGCGGATGT


RET
NM_020630
2
10
+
0
+
0
37
1
0
CGCGAATGCCTGGCATTGGGCCTCTACTTCTCGA
127












GGGATGCTTACACGCGTGGCGGATGT


RET
NM_020630
2
10
+
35
−
0
37
1
0
CGCGAATGCCGTGCCGGCTGCCTGGTCCACATA
128












CAGCTTCTCCCAACGCGTGGCGGATGT


RET
NM_020630
2
10
+
70
+
0
37
1
0
CGCGAATGCCGCCCTTGCTGTACGTCCATGCCCT
129












GCGGGACGCCCACGCGTGGCGGATGT


RET
NM_020630
2
10
+
105
−
0
37
1
0
CGCGAATGCCATGCTGGCCCAGGCGGAAGCTGG
130












GCACCTCCTCAGACGCGTGGCGGATGT


RET
NM_020630
2
10
+
140
+
0
37
1
0
CGCGAATGCCCTCTACGGCACGTACCGCACACG
131












GCTGCATGAGAAACGCGTGGCGGATGT


RET
NM_020630
2
10
+
175
−
0
37
1
0
CGCGAATGCCGGAGGCCGGTGTCCTCCTGGATG
132












CAGATCCAGTTGACGCGTGGCGGATGT


RET
NM_020630
2
10
+
210
+
0
37
1
0
CGCGAATGCCTCTACCTTAACCGGAGCCTGGAC
133












CATAGCTCCTGGACGCGTGGCGGATGT


RET
NM_020630
2
10
+
245
−
0
37
1
0
CGCGAATGCCGGGGCGGCTCCCTTACTGCGGAC
134












ACTGAGCTTCTCACGCGTGGCGGATGT


PIK3CA
NM_006218
10
3
+
−8
+
0
0
2
0
CGCGAATGCCTTTTACAGAGTAACAGACTAGCT
135












AGAGACAATGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
10
3
+
27
−
0
0
2
0
CGCGAATGCCATTGCTTTGAGCTGTTCTTTGTCA
136












TTTTCCCTTAAACGCGTGGCGGATGT


PIK3CA
NM_006218
10
3
+
62
+
0
23
1
1
CGCGAATGCCTTCTACACGAGATCCTCTCTCTGA
137












AATCACTGAGCACGCGTGGCGGATGT


PIK3CA
NM_006218
10
3
+
97
−
0
37
1
0
CGCGAATGCCCACTTACCTGTGACTCCATAGAA
138












AATCTTTCTCCTACGCGTGGCGGATGT


PDGFRA
NM_006206
11
4
+
−22
+
0
37
1
0
CGCGAATGCCGCCTCTCTCTCTTGTCACGTAGCC
139












CTGCGTTCTGAACGCGTGGCGGATGT


PDGFRA
NM_006206
11
4
+
13
−
0
37
1
0
CGCGAATGCCCCAACAGCACCAGGACTGCAGCA
140












GCCACCGTGAGTACGCGTGGCGGATGT


PDGFRA
NM_006206
11
4
+
48
+
0
37
1
0
CGCGAATGCCTGATTGTGATCATCTCACTTATTG
141












TCCTGGTTGTCACGCGTGGCGGATGT


PDGFRA
NM_006206
11
4
+
83
−
0
37
1
0
CGCGAATGCCTAGTTTTATGAGAAAATATCTAC
142












CTGTTTCCAAATACGCGTGGCGGATGT


NFKB1
NM_003998
4
4
+
−50
+
0
37
1
0
CGCGAATGCCGAGAAGCCTCACAGTTTCTTTTG
143












GTTTCTGTTTGTACGCGTGGCGGATGT


NFKB1
NM_003998
4
4
+
−15
−
0
37
1
0
CGCGAATGCCTTGAAGGTATGGGCCATCTGCTA
144












AAAACAAAAACAACGCGTGGCGGATGT


NFKB1
NM_003998
4
4
+
20
+
0
37
1
0
CGCGAATGCCATATTAGAGCAACCTAAACAGGT
145












AAGATTAAAGGGACGCGTGGCGGATGT


NFKB1
NM_003998
4
4
+
55
−
0
37
1
0
CGCGAATGCCTATTAGACACTGGAATCTAACAT
146












TTAAAGTCCCACACGCGTGGCGGATGT


EPHA4
NM_004438
9
2
−
−13
+
0
37
1
0
CGCGAATGCCGTTTATCACTCAGGTGTAAGAAC
147












ATATGTGGACCCACGCGTGGCGGATGT


EPHA4
NM_004438
9
2
−
22
−
0
37
1
0
CGCGAATGCCCTCGCACTGCTTGGTTGGGATCTT
148












CGTACGTAAAGACGCGTGGCGGATGT


EPHA4
NM_004438
9
2
−
57
+
0
37
1
0
CGCGAATGCCAGTTTGCCAAAGAAATTGACGCA
149












TCCTGCATTAAGACGCGTGGCGGATGT


EPHA4
NM_004438
9
2
−
92
−
0
37
1
0
CGCGAATGCCCTCAGACACTTACCAACTCCTAT
150












AACTTTTTCAATACGCGTGGCGGATGT


PALB2
NM_024675
4
16
−
−12
+
0
37
1
0
CGCGAATGCCTTTTAATTACAGAGGCAAAGAAA
151












ACCAATTTTTGAACGCGTGGCGGATGT


PALB2
NM_024675
4
16
−
23
−
0
37
1
0
CGCGAATGCCCTCAGCAAAAGTTAGTATAGTCT
152












CCTCAGGGGGCAACGCGTGGCGGATGT


PALB2
NM_024675
4
16
−
58
+
0
37
1
0
CGCGAATGCCGTCCAAGGGATGCAAGAAGCTCT
153












GCTTGGTACTACACGCGTGGCGGATGT


PALB2
NM_024675
4
16
−
89
−
11
37
1
0
CGCGAATGCCAGCTTACCAAATAACAATGTTGT
154












TCATAATAGTAGACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
0
+
0
37
1
0
CGCGAATGCCTGGGAAGAGATCAGTGGTGTGGA
155












TGAACATTACACACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
35
−
0
37
1
0
CGCGAATGCCCCATGACATTGCACACCTGGTAA
156












GTCCTGATGGGTACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
70
+
0
37
1
0
CGCGAATGCCACCACAGTCAAAACAATTGGCTG
157












AGAACAAACTGGACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
105
−
0
37
1
0
CGCGAATGCCTCCACATAAATCTTCTGAGCTGA
158












GTTCCTGGGGACACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
140
+
0
37
1
0
CGCGAATGCCGCTCAAGTTCACTCTACGAGACT
159












GCAATAGCATTCACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
175
−
0
37
1
0
CGCGAATGCCGTTGAATGTCTCCTTGCAAGTTCC
160












TAAAACCAATGACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
210
+
0
37
1
0
CGCGAATGCCCTGTACTACATGGAGTCTGATGA
161












TGATCATGGGGTACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
245
−
0
37
1
0
CGCGAATGCCTGTCAATCTTTGTAAACTGATGCT
162












CTCGAAATTTCACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
280
+
0
37
1
0
CGCGAATGCCCCATTGCAGCTGATGAAAGTTTC
163












ACTCAAATGGATACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
315
−
0
37
1
0
CGCGAATGCCATCTCAGTGTTGAGCTTCAGAAT
164












ACGGTCCCCAAGACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
350
+
0
37
1
0
CGCGAATGCCTAGAGAAGTAGGTCCTGTCAACA
165












AGAAGGGATTTTACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
385
−
0
37
1
0
CGCGAATGCCGGCAACACAAGCACCAACATCTT
166












GAAATGCCAAATACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
420
+
0
37
1
0
CGCGAATGCCTTGGTGTCTGTGAGAGTATACTTC
167












AAAAAGTGCCCACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
455
−
0
37
1
0
CGCGAATGCCTGTCTGGAAACATAGCCAGATTC
168












TTCACTGTAAATACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
490
+
0
37
1
0
CGCGAATGCCCGGTACCCATGGACTCCCAGTCC
169












CTGGTGGAGGTTACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
525
−
0
37
1
0
CGCGAATGCCTCTTCCTCCTTAGAATTGTTGACA
170












CAAGACCCTCTACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
560
+
0
37
1
0
CGCGAATGCCTCCTCCAAGGATGTACTGCAGTA
171












CAGAAGGCGAATACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
595
−
0
37
1
0
CGCGAATGCCAGCATTGCAGGAACACTTGCCAA
172












TGGGTACAAGCCACGCGTGGCGGATGT


EPHA3
NM_005233
3
3
+
630
+
0
37
1
0
CGCGAATGCCGGCTATGAAGAAAGAGGTTTTAT
173












GTGCCAAGGTAAACGCGTGGCGGATGT


KSR2
NM_173598
13
12
−
−32
+
6
37
1
0
CGCGAATGCCTATCCATCTTTCTCTCTCTTTATCT
174












TTCTCAGGTTACGCGTGGCGGATGT


KSR2
NM_173598
13
12
−
−1
−
0
37
1
0
CGCGAATGCCGGCTTCTTTGGTGCATTTGTTGTG
175












GCACTTTAACCACGCGTGGCGGATGT


KSR2
NM_173598
13
12
−
34
+
0
37
1
0
CGCGAATGCCCCACCCTGTCATCTTCTGATCATC
176












CACCGAGGAGGACGCGTGGCGGATGT


KSR2
NM_173598
13
12
−
69
−
0
37
1
0
CGCGAATGCCCCCCTGCCCCTAGGGCAGTAAGT
177












GTTAAATAGTTAACGCGTGGCGGATGT


NTRK3
NM_001012338
2
15
−
0
+
0
37
1
0
CGCGAATGCCGTGGGAGGACACACCATGCTCCC
178












CATTCGCTGGATACGCGTGGCGGATGT


NTRK3
NM_001012338
2
15
−
35
−
0
37
1
0
CGCGAATGCCTAGTGAACTTCCGGTACATGATG
179












CTTTCAGGAGGCACGCGTGGCGGATGT


NTRK3
NM_001012338
2
15
−
70
+
0
37
1
0
CGCGAATGCCCAGAGAGTGATGTATGGAGCTTC
180












GGGGTGATCCTCACGCGTGGCGGATGT


NTRK3
NM_001012338
2
15
−
105
−
0
37
1
0
CGCGAATGCCAACCATGGCTGCTTTCCATAGGT
181












GAAGATCTCCCAACGCGTGGCGGATGT


NTRK3
NM_001012338
2
15
−
140
+
0
37
1
0
CGCGAATGCCCCAACTCTCAAACACGGAGGTAA
182












AAAGGGGGTGCGACGCGTGGCGGATGT


RPS6KA1
NM_002953
4
1
+
−29
+
0
37
1
0
CGCGAATGCCCTGCCCTGCTTCCTGCTCTGCCTT
183












CTCAGGTCTTCACGCGTGGCGGATGT


RPS6KA1
NM_002953
4
1
+
6
−
0
37
1
0
CGCGAATGCCTGCCCACTGTCAGGCCGGGTGAC
184












TTTCCGCACCAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
4
1
+
41
+
0
37
1
0
CGCGAATGCCCCTGTATGCTATGAAGGTGCTGA
185












AGAAGGCAACGCACGCGTGGCGGATGT


RPS6KA1
NM_002953
4
1
+
76
−
0
37
1
0
CGCGAATGCCGTTCTGCACAGGGAGGTGTCCCC
186












ACTCACCTTTCAACGCGTGGCGGATGT


PKN1
NM_002741
7
19
+
0
+
0
37
1
0
CGCGAATGCCGGACCCTGGAGGTACGAGTGGTG
187












GGCTGCAGAGACACGCGTGGCGGATGT


PKN1
NM_002741
7
19
+
35
−
0
37
1
0
CGCGAATGCCGAGGGGGTAGGGTTCCACGGGAT
188












GGTCTCTGGGAGACGCGTGGCGGATGT


PKN1
NM_002741
7
19
+
70
+
0
37
1
0
CGCGAATGCCAATGGGGGGACCTGGGACCCCAG
189












ACAGCCGCCCCCACGCGTGGCGGATGT


PKN1
NM_002741
7
19
+
105
−
0
37
1
0
CGCGAATGCCGCTGTAAAGGCCCCGGGCTGGGC
190












GGCTCAGGAAGGACGCGTGGCGGATGT


PKN1
NM_002741
7
19
+
140
+
0
37
1
0
CGCGAATGCCCGAAGCGGAAGCCTCAGTGGCCG
191












GAGCAGCCTCAAACGCGTGGCGGATGT


PKN1
NM_002741
7
19
+
175
−
0
37
1
0
CGCGAATGCCACGATGCCCACTCACTGGTGTTC
192












TCGGCTTCTGCTACGCGTGGCGGATGT


KSR2
NM_173598
16
12
−
0
+
0
37
1
0
CGCGAATGCCAGCCAAGAAGAAGAGCAAACCC
193












TTGAACCTCAAGAACGCGTGGCGGATGT


KSR2
NM_173598
16
12
−
35
−
0
37
1
0
CGCGAATGCCGGGGATGTTCTCGCAGCTGCCTA
194












CGCTGCTGTGGAACGCGTGGCGGATGT


KSR2
NM_173598
16
12
−
70
+
0
37
1
0
CGCGAATGCCTCTCAGCAGCGCTCCCCGCTGCT
195












GTCCGAGCGCTCACGCGTGGCGGATGT


KSR2
NM_173598
16
12
−
105
−
0
37
1
0
CGCGAATGCCGGAAAGGTGCGTGTCCCACAAAG
196












AAGGAGCGGAGGACGCGTGGCGGATGT


KSR2
NM_173598
16
12
−
140
+
0
37
1
0
CGCGAATGCCTGCCTTCCACCCCTCCTGTTCACA
197












CTGAGGCCAACACGCGTGGCGGATGT


KSR2
NM_173598
16
12
−
175
−
0
37
1
0
CGCGAATGCCAGTGCAGCCCGGCAGGGTGACTT
198












ACTTGCAGAGAAACGCGTGGCGGATGT


CHAF1A
NM_005483
4
19
+
−42
+
0
37
1
0
CGCGAATGCCTGAAATAACCCGTGTTTAAAGAT
199












AAACGTCTTCTGACGCGTGGCGGATGT


CHAF1A
NM_005483
4
19
+
−7
−
0
37
1
0
CGCGAATGCCTAGAGCCTTTGACGAATTTCTTA
200












GTTATCTGAAAAACGCGTGGCGGATGT


CHAF1A
NM_005483
4
19
+
28
+
0
37
1
0
CGCGAATGCCCAGAGAAGAACAAGCTCAGACT
201












GCAAAGAGTAAGAACGCGTGGCGGATGT


CHAF1A
NM_005483
4
19
+
63
−
0
37
1
0
CGCGAATGCCGCACAGCAGGTTAATTTTCTATTT
202












CAGGGAAAATGACGCGTGGCGGATGT


RBBP8
NM_002894
2
18
+
−16
+
0
37
1
0
CGCGAATGCCGAGCATATTAAGCAAGATGAACA
203












TCTCGGGAAGCAACGCGTGGCGGATGT


RBBP8
NM_002894
2
18
+
19
−
0
37
1
0
CGCGAATGCCACTAGATGTATCTGCAGAGTTAG
204












GGCTTCCACAGCACGCGTGGCGGATGT


RBBP8
NM_002894
2
18
+
54
+
0
37
1
0
CGCGAATGCCGACTTTAAGGACCTTTGGACAAA
205












ACTAAAAGAATGACGCGTGGCGGATGT


RBBP8
NM_002894
2
18
+
89
−
0
37
1
0
CGCGAATGCCAAGAAAAGATTTTACCTTGTACT
206












TCTCTATCATGAACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
0
+
0
37
1
0
CGCGAATGCCATGAGCCGGGGCGCGGGCGCGCT
207












TCAGCGCCGGACACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
35
−
0
37
1
0
CGCGAATGCCGCTTAACCAGGGTCAGCGAGATG
208












AGGTAGGTCGTTACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
66
+
27
37
1
0
CGCGAATGCCAAGCTCGAGTCGGTGCCTCCGCC
209












GCCGCCTTCTCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
109
−
23
37
1
0
CGCGAATGCCCTCGGAGCCTCTGGCACCGGCGG
210












CGCCGGCCGCGGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
140
+
0
37
1
0
CGCGAATGCCCGAGACTGGGGATCCTGGCAGCC
211












CCCGAGGCGCGGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
175
−
0
37
1
0
CGCGAATGCCGAAGAGACGTTCGTGCCGCTTCT
212












TGCCCGGCTCCTACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
210
+
0
37
1
0
CGCGAATGCCCACCGGCAGGATGCGCTGTGGAT
213












CAGCACGAGCAGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
245
−
0
37
1
0
CGCGAATGCCACAGGGCTGGGGGCTCCGCGCCC
214












CCGGTGCCCGCGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
280
+
0
37
1
0
CGCGAATGCCCCCCGGCTCCGGCCAGTCCGGCC
215












CGCCCAGTCTCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
315
−
0
37
1
0
CGCGAATGCCACGGCCCAGAGGGAGAGGCGGC
216












GGCCGGGAGCGGGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
350
+
0
37
1
0
CGCGAATGCCCCCTCCGGGACCCCCGCTCTCCG
217












GGGGACTGAGCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
385
−
0
37
1
0
CGCGAATGCCGGAGGAGGTGGGGGCGCCCCCA
218












GGCTTGGGGTCGGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
420
+
0
37
1
0
CGCGAATGCCCGGCGCCCCCTGCTCAGCAGCCC
219












GAGCTGGGGCGGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
455
−
0
37
1
0
CGCGAATGCCGGATGCCGCCGCCCGCCCGGCCT
220












TCGGGCTCCGGGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
490
+
0
37
1
0
CGCGAATGCCCTGGCTCATCCTCTCCGCACCCTG
221












GCACCGGCAGCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
525
−
0
37
1
0
CGCGAATGCCGGAGCCGGCGGAGGAGGCGCCA
222












CCTTGAGCCTCCGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
560
+
0
37
1
0
CGCGAATGCCCAAGCCTTGCAAGACCGTGACCA
223












CGAGTGGAGCCAACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
595
−
0
37
1
0
CGCGAATGCCCAGGCGGCTACCCGCGCCCTTGC
224












CCCCGCCGGCTTACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
630
+
0
37
1
0
CGCGAATGCCTCATGGCCCGAAAGCGAGGGCAA
225












GCCCAGGGTCAAACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
665
−
0
37
1
0
CGCGAATGCCCCGAAGCTCCAGTCCCGGCGCTG
226












CTCTTTGACCCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
700
+
25
37
1
0
CGCGAATGCCTCTCTGCCGCCGCCACCGCCGCC
227












GCCGCCGGGGGAACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
735
−
0
37
1
0
CGCGAATGCCCCGACCCCACCAGAGGTCGAAGC
228












TGTAGAGCCCCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
770
+
0
37
1
0
CGCGAATGCCGGCTGGGGCTGGAGCCCGAGGG
229












AAGTTGTCCCCTCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
805
−
0
37
1
0
CGCGAATGCCGTCACTGTTGTCCAAGGTCTTACT
230












CTTGCCTTTCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
840
+
0
37
1
0
CGCGAATGCCTTGCATCCGGGACCGCCTGCCGG
231












CTCTCCTCCTCCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
875
−
0
37
1
0
CGCGAATGCCCAGTGGCTGGACTCGGAGTTGGT
232












GGGAGGGTTAGCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
910
+
0
37
1
0
CGCGAATGCCCTGTCACCGCTGCTTCCGCGCAG
233












CCCCCCGGGCCTACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
945
−
0
37
1
0
CGCGAATGCCCCCGGAGCTGGAGGCTCCAGAGT
234












GATTGGAGGTGCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
980
+
0
37
1
0
CGCGAATGCCGCTGAAACGGGGCCGGGAGGGG
235












GGCCGAGCATCCAACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
1015
−
0
37
1
0
CGCGAATGCCGCCGCTGATAAACTTGAGCATCT
236












TGCGGTCACGAGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
1050
+
0
37
1
0
CGCGAATGCCATCTTCACCAAGAGCACAGGAGG
237












GCCTCCTGGCTCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
1085
−
0
37
1
0
CGCGAATGCCCAGAAGACAGGCTGGGGGGTCC
238












GGGAAGGGGCCCGACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
1120
+
0
37
1
0
CGCGAATGCCGCAGCGGGTCCAGGGAGCTGCTG
239












GGCGCCGAGCTCACGCGTGGCGGATGT


CENTG1
NM_001122772
19
12
−
1155
−
0
37
1
0
CGCGAATGCCCTCAAGCCCTGACTCAACTCACT
240












AGGGGAAGCGCGACGCGTGGCGGATGT


PKN1
NM_002741
1
19
+
−56
+
2
37
1
0
CGCGAATGCCGCTCCTCTGGCCGCCCCTCCCTCC
241












GCGCGGGGACCACGCGTGGCGGATGT


PKN1
NM_002741
1
19
+
−25
−
0
37
1
0
CGCGAATGCCCGCTGGCCATGTCCTCCTGCCGC
242












CCGCCAGGGGTCACGCGTGGCGGATGT


PKN1
NM_002741
1
19
+
9
+
0
37
1
0
CGCGAATGCCGACGCCGTGCAGGTAGGCGCACC
243












TGCGTCTGGAGTACGCGTGGCGGATGT


PKN1
NM_002741
1
19
+
41
−
32
37
1
0
CGCGAATGCCGGGGTCCGCCGGCGCCGTCCGGT
244












CGCCCCGGGACTACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
0
+
0
37
1
0
CGCGAATGCCTATATAAGAAGCTGTATAATGCT
245












TGGGAGGATGCCACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
35
−
0
37
1
0
CGCGAATGCCAATAAAGGCTTTCTTTAGCCATC
246












AACATCAAATTGACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
70
+
0
37
1
0
CGCGAATGCCCTCAACTGCCAATGGACTGTTTT
247












ACAATGCCATCTACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
105
−
0
37
1
0
CGCGAATGCCATATATGGTGTAGCTGTGGAAAT
248












GCGTCTGGAATAACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
140
+
0
37
1
0
CGCGAATGCCGAATGGAGAAACATCTACAAAAT
249












CCCTTTGGGTTAACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
175
−
0
37
1
0
CGCGAATGCCTGCACAAAGAATTTTTATTCTGA
250












GTGCACTATTTAACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
210
+
0
37
1
0
CGCGAATGCCACCTACGTGAATGTAAATATTCG
251












AGACATTGATAAACGCGTGGCGGATGT


PIK3CA
NM_006218
5
3
+
245
−
0
37
1
0
CGCGAATGCCCTATAAAATAATAAGCATCAGCA
252












TTTGACTTTACCACGCGTGGCGGATGT


KSR2
NM_173598
12
12
−
−8
+
0
37
1
0
CGCGAATGCCCCCTACGGATCCAGCAAGGTTAG
253












TCCGGACAGAGTACGCGTGGCGGATGT


KSR2
NM_173598
12
12
−
27
−
0
37
1
0
CGCGAATGCCCTTCCGTAGAGGGTTGTTGATGT
254












CACACGGAACGGACGCGTGGCGGATGT


KSR2
NM_173598
12
12
−
62
+
0
37
1
0
CGCGAATGCCCCACCTCGCTATTCAGACCTGCA
255












CATCAGTCAGACACGCGTGGCGGATGT


KSR2
NM_173598
12
12
−
97
−
0
37
1
0
CGCGAATGCCGCCTTACCTTGTTGATTTTGTTGG
256












TTTTGGGGAGCACGCGTGGCGGATGT


PDGFRA
NM_006206
2
4
+
−46
+
0
37
1
0
CGCGAATGCCTAATGCTGTTTCTGTTGACTTTTG
257












ACTTTTCTAGTACGCGTGGCGGATGT


PDGFRA
NM_006206
2
4
+
−11
−
0
37
1
0
CGCGAATGCCGAACGCCGGATGGGAAGTCCCCA
258












TAGCTCTGGGAAACGCGTGGCGGATGT


PDGFRA
NM_006206
2
4
+
24
+
0
37
1
0
CGCGAATGCCCTGGTCTTAGGCTGTCTTCTCACA
259












GGTACGGAGCCACGCGTGGCGGATGT


PDGFRA
NM_006206
2
4
+
59
−
0
37
1
0
CGCGAATGCCACAAGACACCCAAACAAGGAAC
260












TCAGAGAGGACTGACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
0
+
0
37
1
0
CGCGAATGCCGTCCACCATCTGCTCCCCTGAACT
261












TGATTTCAAATACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
35
−
0
37
1
0
CGCGAATGCCCTACTCCATTCCAAGTTCACAGA
262












TGTCTCGTTGACACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
70
+
0
37
1
0
CGCGAATGCCCCCTCAGAATACAGGTGGCCGCC
263












AGGACATTTCCTACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
105
−
0
37
1
0
CGCGAATGCCGTCACCAGCTCCACATTTCTTGCA
264












TACCACATTATACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
140
+
0
37
1
0
CGCGAATGCCCCCAGCAAGTGCCGACCCTGTGG
265












AAGTGGGGTCCAACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
175
−
0
37
1
0
CGCGAATGCCTGGTGGTCTTCAAGCCATTCTGCT
266












GTGGGGTGTAGACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
210
+
0
37
1
0
CGCGAATGCCAAGTCTCCATCACTGACCTCCTA
267












GCTCATACCAATACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
245
−
0
37
1
0
CGCGAATGCCGACACTCCATTCACAGCCCAGAT
268












TTCAAAGGTGTAACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
280
+
0
37
1
0
CGCGAATGCCCAAATATAACCCTAACCCAGACC
269












AATCAGTTTCTGACGCGTGGCGGATGT


EPHA4
NM_004438
14
2
−
315
−
0
37
1
0
CGCGAATGCCTCAATTCCTACCTGCTTGGTTGGT
270












GGTCACAGTGAACGCGTGGCGGATGT


EPHA3
NM_005233
11
3
+
0
+
0
37
1
0
CGCGAATGCCGTGAATTTGGAGAGGTGTGCAGT
271












GGTCGCTTAAAAACGCGTGGCGGATGT


EPHA3
NM_005233
11
3
+
35
−
0
37
1
0
CGCGAATGCCTTAATGGCCACTGAAATCTCTTTT
272












TTTGAAGGAAGACGCGTGGCGGATGT


EPHA3
NM_005233
11
3
+
70
+
0
37
1
0
CGCGAATGCCGACCCTGAAAGTTGGCTACACAG
273












AAAAGCAGAGGAACGCGTGGCGGATGT


EPHA3
NM_005233
11
3
+
105
−
0
37
1
0
CGCGAATGCCCTGTCCCATAATGCTTGCTTCTCC
274












CAGGAAGTCTCACGCGTGGCGGATGT


EPHA3
NM_005233
11
3
+
140
+
0
37
1
0
CGCGAATGCCTTTGACCACCCCAATATCATTCG
275












ACTGGAAGGAGTACGCGTGGCGGATGT


EPHA3
NM_005233
11
3
+
175
−
0
37
1
0
CGCGAATGCCAGGTCTTATGACTACTTTACTTAC
276












TTTTGGTAACAACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
0
+
0
37
1
0
CGCGAATGCCACATTCAAACGTGTTTTGATCAA
277












AGAAGAGGAGTAACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
35
−
0
37
1
0
CGCGAATGCCTGAAGACCGAGTTATAGAATACT
278












ATAATAGAATCAACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
70
+
0
37
1
0
CGCGAATGCCTGCAGAGACTGAAAACAAATATT
279












TTGCAGTATGCTACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
105
−
0
37
1
0
CGCGAATGCCCCAATCAAAGGATACTTTTGACC
280












TACCCTGGTGGAACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
140
+
0
37
1
0
CGCGAATGCCAAAAATCTAATGTAATGGGTCCA
281












CCAAAACATTAAACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
175
−
0
37
1
0
CGCGAATGCCGGGCTAGAGCAAAAACAAAAAA
282












GTAGATTATTTATACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
210
+
0
37
1
0
CGCGAATGCCCCTACCTTGTCACCAATACCTCAC
283












ATTCCTCGAAGACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
245
−
0
37
1
0
CGCGAATGCCGAATCCGTAAGGGTGAACTAGGA
284












AACTTGTAAGGGACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
280
+
0
37
1
0
CGCGAATGCCCTGGAGGGAACATCTATATTTCA
285












CCCCTGAAGAGTACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
315
−
0
37
1
0
CGCGAATGCCGTTGGTGTTGGCAGACCTTCTGA
286












AATTTTATATGGACGCGTGGCGGATGT


RB1
NM_000321
23
13
+
350
+
0
37
1
0
CGCGAATGCCAAAAATGACTCCAAGATCAAGGT
287












GTGTGTTTTCTCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
0
+
0
37
1
0
CGCGAATGCCTGGGAAGAAGTCAGTGGCTACGA
288












TGAAAACCTGAAACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
35
−
0
37
1
0
CGCGAATGCCCGAAGACATTGCACACCTGGTAG
289












GTGCGGATGGTGACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
70
+
0
37
1
0
CGCGAATGCCAGCCCAACCAGAACAATTGGCTG
290












CTCACCACCTTCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
105
−
0
37
1
0
CGCGAATGCCTCTGTGTAGATGCGATGGGCCCC
291












CCGCCGGTTGATACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
140
+
0
37
1
0
CGCGAATGCCGATGCGCTTCACTGTGAGAGACT
292












GCAGCAGCCTCCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
175
−
0
37
1
0
CGCGAATGCCGTTGAAGGTCTCCTTGCAGGATC
293












CTGGGACATTAGACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
210
+
0
37
1
0
CGCGAATGCCTTGTATTACTATGAGACTGACTCT
294












GTCATTGCCACACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
245
−
0
37
1
0
CGCGAATGCCGGTAGGGGGCCTCAGACCAGAA
295












GGCTGACTTCTTGACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
280
+
0
37
1
0
CGCGAATGCCTCAAAGTAGACACCATTGCTGCA
296












GATGAGAGCTTCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
315
−
0
37
1
0
CGCGAATGCCACCTTCATCAGCCTTCCCCCAAA
297












GTCCACCTGGGAACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
350
+
0
37
1
0
CGCGAATGCCAAACACAGAAGTCAGGAGCTTTG
298












GGCCTCTTACTCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
385
−
0
37
1
0
CGCGAATGCCTCCATAATCCTGAAAAGCGAGGT
299












AAAAACCATTCCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
420
+
0
37
1
0
CGCGAATGCCGCCTGTATGTCTCTTCTTTCTGTC
300












CGTGTCTTCTTACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
455
−
0
37
1
0
CGCGAATGCCCTGCAAAATTTTGCACAATGCTG
301












GGACACTTTTTGACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
490
+
0
37
1
0
CGCGAATGCCTGTTTCCAGAGACTATGACAGGG
302












GCAGAGAGCACAACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
525
−
0
37
1
0
CGCGAATGCCTTGGGGATGCATGTGCCCCGAGC
303












AATCACCAGAGAACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
560
+
0
37
1
0
CGCGAATGCCCGCAGAGGAAGTGGACGTGCCCA
304












TCAAACTCTACTACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
595
−
0
37
1
0
CGCGAATGCCCCCAATAGGCACCATCCATTCCC
305












CATCCCCGTTGCACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
630
+
0
37
1
0
CGCGAATGCCCGATGCACCTGCAAGCCTGGCTA
306












TGAGCCTGAGAAACGCGTGGCGGATGT


EPHB1
NM_004441
3
3
+
665
−
0
37
1
0
CGCGAATGCCAGAGGCTCCAAAGCTTACCCTTG
307












CATGCCACGCTGACGCGTGGCGGATGT


NFKB1
NM_003998
22
4
+
0
+
0
37
1
0
CGCGAATGCCGAGACATGAAACAGCTGGCTGAA
308












GATGTGAAGCTGACGCGTGGCGGATGT


NFKB1
NM_003998
22
4
+
35
−
0
37
1
0
CGCGAATGCCTCTGGATCAGGAATTTCTAGTAA
309












CTTATACAGCTGACGCGTGGCGGATGT


NFKB1
NM_003998
22
4
+
70
+
0
37
1
0
CGCGAATGCCCAAAAACTGGGCTACTCTGGCGC
310












AGAAATTAGGTCACGCGTGGCGGATGT


NFKB1
NM_003998
22
4
+
105
−
0
37
1
0
CGCGAATGCCAGGACTCAGCCGGAAGGCATTAT
311












TAAGTATCCCCAACGCGTGGCGGATGT


NFKB1
NM_003998
22
4
+
140
+
0
37
1
0
CGCGAATGCCGCTCCTTCCAAAACACTTATGGA
312












CAACTATGAGGTACGCGTGGCGGATGT


RBBP8
NM_002894
13
18
+
−26
+
0
37
1
0
CGCGAATGCCTTGCATGCTCTTTCCCTTTACCTA
313












AGATGTATCCTACGCGTGGCGGATGT


RBBP8
NM_002894
13
18
+
9
−
0
37
1
0
CGCGAATGCCTGCTCCCGGATCTATACTCCACTG
314












GATATTTTCAAACGCGTGGCGGATGT


RBBP8
NM_002894
13
18
+
44
+
0
37
1
0
CGCGAATGCCGACCTTTCTCAGTATAAAATGGA
315












TGTTACTGTAATACGCGTGGCGGATGT


RBBP8
NM_002894
13
18
+
79
−
0
37
1
0
CGCGAATGCCGTTTTGGTTTTACTTTTTAACTTA
316












CCTTTGTATCTACGCGTGGCGGATGT


NFKB1
NM_003998
7
4
+
0
+
0
37
1
0
CGCGAATGCCCTTCGCAAACCTGGGTATACTTC
317












ATGTGACAAAGAACGCGTGGCGGATGT


NFKB1
NM_003998
7
4
+
35
−
0
37
1
0
CGCGAATGCCTGTCATTCGTGCTTCCAGTGTTTC
318












AAATACTTTTTACGCGTGGCGGATGT


NFKB1
NM_003998
7
4
+
70
+
0
37
1
0
CGCGAATGCCGAGGCGTGTATAAGGGGCTATAA
319












TCCTGGACTCTTACGCGTGGCGGATGT


NFKB1
NM_003998
7
4
+
105
−
0
37
1
0
CGCGAATGCCCTTCTGCTTGCAAATAGGCAAGG
320












TCAGGGTGCACCACGCGTGGCGGATGT


NFKB1
NM_003998
7
4
+
140
+
0
37
1
0
CGCGAATGCCGTGGAGGGGACCGGCAGCTGGG
321












AGGTAAGCATCATACGCGTGGCGGATGT


NFKB1
NM_003998
10
4
+
−24
+
0
37
1
0
CGCGAATGCCACACTTCAATGTGATTGTTTGCA
322












GATGACATCCAGACGCGTGGCGGATGT


NFKB1
NM_003998
10
4
+
11
−
0
37
1
0
CGCGAATGCCACTCCACCATTTTCTTCCTCTTCA
323












TAAAATCGAATACGCGTGGCGGATGT


NFKB1
NM_003998
10
4
+
46
+
0
37
1
0
CGCGAATGCCCTGGGAAGGATTTGGAGATTTTT
324












CCCCCACAGATGACGCGTGGCGGATGT


NFKB1
NM_003998
10
4
+
81
−
0
37
1
0
CGCGAATGCCTAATAATAATAATAAATCACTTA
325












CTTGTCTATGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
0
+
0
37
1
0
CGCGAATGCCATGCCTCCACGACCATCATCAGG
326












TGAACTGTGGGGACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
35
−
0
37
1
0
CGCGAATGCCATTCTACTAGGATTCTTGGGGGC
327












ATCAAGTGGATGACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
70
+
0
37
1
0
CGCGAATGCCGTTTACTACCAAATGGAATGATA
328












GTGACTTTAGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
105
−
0
37
1
0
CGCGAATGCCTGCTTTATGGTTATTAATGTAGCC
329












TCACGGAGGCAACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
140
+
0
37
1
0
CGCGAATGCCTGAACTATTTAAAGAAGCAAGAA
330












AATACCCCCTCCACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
175
−
0
37
1
0
CGCGAATGCCGAAAATGTAAGAAGATTCATCTT
331












GAAGAAGTTGATACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
210
+
0
37
1
0
CGCGAATGCCGTAAGTGTTACTCAAGAAGCAGA
332












AAGGGAAGAATTACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
245
−
0
37
1
0
CGCGAATGCCGCCGAAGGTCACAAAGTCGTCTT
333












GTTTCATCAAAAACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
280
+
0
37
1
0
CGCGAATGCCTTTTTCAACCCTTTTTAAAAGTAA
334












TTGAACCAGTAACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
315
−
0
37
1
0
CGCGAATGCCATTTCTCGATTGAGGATCTTTTCT
335












TCACGGTTGCCACGCGTGGCGGATGT


PIK3CA
NM_006218
2
3
+
350
+
0
37
1
0
CGCGAATGCCTGGTATGATACAATATCCTATTCT
336












AAAATGCAAATACGCGTGGCGGATGT


EPHA4
NM_004438
10
2
−
−44
+
13
37
1
0
CGCGAATGCCGTGTTTGAGGAAAATAATTCTCT
337












TTTTTAAAAAAAACGCGTGGCGGATGT


EPHA4
NM_004438
10
2
−
−1
−
0
37
1
0
CGCGAATGCCCGCTTCTTGTTTGGCTTTACTGTA
338












TTTACTCCGTCACGCGTGGCGGATGT


EPHA4
NM_004438
10
2
−
30
+
0
37
1
0
CGCGAATGCCAGCGGATGAAGAGAAACATTTGA
339












ATCAAGGTACAAACGCGTGGCGGATGT


EPHA4
NM_004438
10
2
−
65
−
0
37
1
0
CGCGAATGCCCATGCTCTAAAAATAGAATTTTTT
340












AATCCAATTTTACGCGTGGCGGATGT


NFKB1
NM_003998
23
4
+
0
+
0
37
1
0
CGCGAATGCCGTCTCTGGGGGTACAGTCAGAGA
341












GCTGGTGGAGGCACGCGTGGCGGATGT


NFKB1
NM_003998
23
4
+
35
−
0
37
1
0
CGCGAATGCCCTTCAATTGCTTCGGTGTAGCCCA
342












TTTGTCTCAGGACGCGTGGCGGATGT


NFKB1
NM_003998
23
4
+
70
+
0
37
1
0
CGCGAATGCCTGATCCAGGCAGCCTCCAGCCCA
343












GTGAAGACCACCACGCGTGGCGGATGT


NFKB1
NM_003998
23
4
+
105
−
0
37
1
0
CGCGAATGCCGAGGCAGGCGAGAGAGGCAGCG
344












AGTGGGCCTGAGAACGCGTGGCGGATGT


NFKB1
NM_003998
23
4
+
140
+
0
37
1
0
CGCGAATGCCCACAAGGCAGCAAATAGGTAAA
345












AAAAAAGACAAAAACGCGTGGCGGATGT


NFKB1
NM_003998
20
4
+
−8
+
0
37
1
0
CGCGAATGCCCTTCGCAGGAGCAGATCCCCTGG
346












TGGAGAACTTTGACGCGTGGCGGATGT


NFKB1
NM_003998
20
4
+
27
−
0
37
1
0
CGCGAATGCCATTTTCCCAAGAGTCATCCAGGT
347












CATAGAGAGGCTACGCGTGGCGGATGT


NFKB1
NM_003998
20
4
+
62
+
0
37
1
0
CGCGAATGCCGCAGGAGAGGATGAAGGAGTTG
348












TGCCTGGAACCACACGCGTGGCGGATGT


NFKB1
NM_003998
20
4
+
97
−
0
37
1
0
CGCGAATGCCCACTCACCTGCCAGCTGGTGGCC
349












ATATCTAGAGGCACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
0
+
0
37
1
0
CGCGAATGCCGGCAAATAATAGTGGTGATCTGG
350












GTAATAGTTTCTACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
35
−
0
37
1
0
CGCGAATGCCATTTTCAGAGTATACTTCTGCTTG
351












TCATTATTTGGACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
70
+
0
37
1
0
CGCGAATGCCCAACCATGACTGTGTACCAGAAC
352












AAGTAATTGCTGACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
105
−
0
37
1
0
CGCGAATGCCTAGCAACATACTTCGAGTTTTTTT
353












CCTGATTGCTTACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
140
+
0
37
1
0
CGCGAATGCCTCCTCTGAACAACTAAAACTCTG
354












TGTTTTAGAATAACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
175
−
0
37
1
0
CGCGAATGCCCACATCCACACACTTTTAAAATA
355












TACTTGCCCTGAACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
210
+
0
37
1
0
CGCGAATGCCATGAATACTTCCTAGAAAAATAT
356












CCTCTGAGTCAGACGCGTGGCGGATGT


PIK3CA
NM_006218
4
3
+
241
−
9
37
1
0
CGCGAATGCCTAATATTTTGAAACTTGTTACTCA
357












CCTTATACTGAACGCGTGGCGGATGT


GUCY2F
NM_001522
4
X
−
−22
+
0
37
1
0
CGCGAATGCCCATGTCTCTCCTTCTTTTGCAGCT
358












TATCGCATTCAACGCGTGGCGGATGT


GUCY2F
NM_001522
4
X
−
13
−
0
37
1
0
CGCGAATGCCGATTTTGAAGAATTGTAACAGTG
359












CTGAGACTGACAACGCGTGGCGGATGT


GUCY2F
NM_001522
4
X
−
48
+
0
37
1
0
CGCGAATGCCTGAGTGAGGGCTATGAAGTGGAG
360












CTTCGAGGAAGAACGCGTGGCGGATGT


GUCY2F
NM_001522
4
X
−
83
−
0
37
1
0
CGCGAATGCCCAAAAAACAAAAAGACATTATAC
361












CTTGAGCTCTGTACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
0
+
0
37
1
0
CGCGAATGCCTCCCCATTCCCAGGGGATGATGA
362












GGAGGAGGTCTTACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
35
−
0
37
1
0
CGCGAATGCCGGGGGTAGCGAACCTCGTCGTTG
363












ACGATGCTGTCGACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
70
+
0
37
1
0
CGCGAATGCCGCTTCCTGTCGGCCGAAGCCATC
364












GGCATCATGAGAACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
105
−
0
37
1
0
CGCGAATGCCCCCCAGGCCCGCCCCACGTCGGG
365












GGTCCTCACCCTACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
140
+
0
37
1
0
CGCGAATGCCAGGGGCAGTGGGGCCCAGGAGG
366












GGACAGATCCTGAACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
175
−
0
37
1
0
CGCGAATGCCTTCCTCCGAAGCAGCTTGGAGGA
367












AGGAGCACTGTGACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
210
+
0
37
1
0
CGCGAATGCCCCCAGAGCGGAGGCTGGGATCTA
368












GCGAGAGAGATGACGCGTGGCGGATGT


PKN1
NM_002741
21
19
+
245
−
0
37
1
0
CGCGAATGCCCACCCTGAAGAAGGGCTGTTTCT
369












TCACATCTTCTGACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
0
+
0
37
1
0
CGCGAATGCCATATGATGCAGCCATTGACCTGT
370












TTACACGTTCATACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
35
−
0
37
1
0
CGCGAATGCCTCCCAAAATGAAGGTAGCTACAC
371












AGTATCCAGCACACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
70
+
0
37
1
0
CGCGAATGCCATTGGAGATCGTCACAATAGTAA
372












CATCATGGTGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
105
−
0
37
1
0
CGCGAATGCCTTTAAACAGAGAAAACCATTACT
373












TGTCCATCGTCTACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
140
+
0
37
1
0
CGCGAATGCCATGTTTTGGTGTTCTTAATTTATT
374












CAAGACATTTTACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
175
−
0
37
1
0
CGCGAATGCCAAGAAATTATGTTATAGTTTGAT
375












ATATGCAGATACACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
210
+
0
37
1
0
CGCGAATGCCATTTTTGAAAGCTGTTTCATATAG
376












ATTTTGGACACACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
245
−
0
37
1
0
CGCGAATGCCTTATAACCAAATTTTTTCTTCTTG
377












TGATCCAAAAAACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
280
+
0
37
1
0
CGCGAATGCCACGAGAACGTGTGCCATTTGTTT
378












TGACACAGGATTACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
315
−
0
37
1
0
CGCGAATGCCGCATTCTTGGGCTCCTTTACTAAT
379












CACTATTAAGAACGCGTGGCGGATGT


PIK3CA
NM_006218
20
3
+
350
+
0
37
1
0
CGCGAATGCCACAAAGACAAGAGAATTTGAGA
380












GGTGAGCTCGAGCACGCGTGGCGGATGT


RBBP8
NM_002894
4
18
+
−22
+
0
37
1
0
CGCGAATGCCTTATTTATTTTTTGACCTTTAGAG
381












ATGCACAAAGAACGCGTGGCGGATGT


RBBP8
NM_002894
4
18
+
13
−
0
37
1
0
CGCGAATGCCCTCAGCTGTTGATTTTTGGTGAAG
382












AATTCTTCTAGACGCGTGGCGGATGT


RBBP8
NM_002894
4
18
+
48
+
0
37
1
0
CGCGAATGCCGGAACAGCAGAAAGTCCTTCATG
383












AAACCATTAAAGACGCGTGGCGGATGT


RBBP8
NM_002894
4
18
+
83
−
0
37
1
0
CGCGAATGCCAAGACCTAAGTGCCAGACTCACC
384












GATCTTCTAAAAACGCGTGGCGGATGT


NTRK3
NM_001012338
8
15
−
−18
+
0
37
1
0
CGCGAATGCCGTTTCTGGTTCCTCACAGGTATCC
385












ATAGCAGTTGGACGCGTGGCGGATGT


NTRK3
NM_001012338
8
15
−
17
−
0
37
1
0
CGCGAATGCCGAACCACCAACAGGACACAGGC
386












AAAAGCAGCAAGTACGCGTGGCGGATGT


NTRK3
NM_001012338
8
15
−
52
+
0
37
1
0
CGCGAATGCCTCTTCGTCATGATCAACAAATAT
387












GGTCGACGGTCCACGCGTGGCGGATGT


NTRK3
NM_001012338
8
15
−
87
−
0
37
1
0
CGCGAATGCCAATGAAACTCCCACCTTACCCTT
388












CATTCCAAATTTACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
0
+
0
37
1
0
CGCGAATGCCAAAAATGATGGCAGATTTACAGT
389












CATTCAGCTGGTACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
35
−
0
37
1
0
CGCGAATGCCACTTCATCCCAGACCCAATGCCA
390












CGAAGCATGCCCACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
70
+
0
37
1
0
CGCGAATGCCATTTATCTGATATGAGCTATGTGC
391












ATCGTGATCTGACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
105
−
0
37
1
0
CGCGAATGCCACCAAGTTGCTGTTCACCAGGAT
392












GTTCCGTGCGGCACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
140
+
0
37
1
0
CGCGAATGCCCTGCAAAGTGTCTGATTTTGGCA
393












TGTCCCGAGTGCACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
175
−
0
37
1
0
CGCGAATGCCCCTGGTGGTGTAAGCTGCTTCCG
394












GATCATCCTCAAACGCGTGGCGGATGT


EPHA4
NM_004438
6
2
−
210
+
0
37
1
0
CGCGAATGCCGTAAGAAAGATCGGTGACATCTG
395












GGCTTTCACTCTACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
0
+
0
37
1
0
CGCGAATGCCATGGATGTCTCTCTTTGCCCAGCC
396












AAGTGTAGTTTACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
35
−
0
37
1
0
CGCGAATGCCCCAGCCAGACGCTTCCCAGCAAG
397












AAAATCCGCCAGACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
70
+
0
37
1
0
CGCGAATGCCACTATGTGGGCTCCGTGCTGGCT
398












TGCCCTGCAAATACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
105
−
0
37
1
0
CGCGAATGCCCGCCGGCAATTGATCTCAGTCTT
399












GCTGCAGACACAACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
140
+
0
37
1
0
CGCGAATGCCGCCGGACGATGGGAACCTCTTCC
400












CCCTCCTGGAAGACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
175
−
0
37
1
0
CGCGAATGCCACTGGCGTTCCCATTGCTGTTCCC
401












TGAATCCTGCCACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
210
+
0
37
1
0
CGCGAATGCCATCAACATCACGGACATCTCAAG
402












GAATATCACTTCACGCGTGGCGGATGT


NTRK3
NM_001012338
18
15
−
245
−
0
37
1
0
CGCGAATGCCGAGGCAGGCTGGGGAGCGGCCG
403












CCTGACTTACATGACGCGTGGCGGATGT


PIK3CA
NM_006218
7
3
+
−17
+
0
37
1
0
CGCGAATGCCTTTAAAATTTTACATAGGTGGAA
404












TGAATGGCTGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
7
3
+
18
−
0
37
1
0
CGCGAATGCCCAGCACGAGGAAGATCAGGAAT
405












GTATATATCATAAACGCGTGGCGGATGT


PIK3CA
NM_006218
7
3
+
53
+
0
37
1
0
CGCGAATGCCCTCGACTTTGCCTTTCCATTTGCT
406












CTGTTAAAGGCACGCGTGGCGGATGT


PIK3CA
NM_006218
7
3
+
88
−
0
37
1
0
CGCGAATGCCCTTCTGAAATACTTTACCTCTTTA
407












GCACCCTTTCGACGCGTGGCGGATGT


NTRK3
NM_001012338
15
15
−
−36
+
0
37
1
0
CGCGAATGCCTGTTTTAAAATTTCCCTTTATTTG
408












TCAATCTTGCAACGCGTGGCGGATGT


NTRK3
NM_001012338
15
15
−
−1
−
0
37
1
0
CGCGAATGCCCGAGAGTGTGGTGAGCCGGTTAC
409












TTGACAGGTTTCACGCGTGGCGGATGT


NTRK3
NM_001012338
15
15
−
34
+
0
37
1
0
CGCGAATGCCTGGCAGCTCTTCCAGACGCTGAG
410












TCTTCGGGAATTACGCGTGGCGGATGT


NTRK3
NM_001012338
15
15
−
69
−
0
37
1
0
CGCGAATGCCTTCAGTACCTGGACAGTCTTCAA
411












AACCAAACTTACACGCGTGGCGGATGT


PALB2
NM_024675
6
16
−
−27
+
0
37
1
0
CGCGAATGCCAATTTTTGGCTGCTTTGTTTTATT
412












TAGGTTCCAGTACGCGTGGCGGATGT


PALB2
NM_024675
6
16
−
8
−
0
37
1
0
CGCGAATGCCGATTATACACATCAGGCACTGGA
413












ACTATCTGTAATACGCGTGGCGGATGT


PALB2
NM_024675
6
16
−
43
+
0
37
1
0
CGCGAATGCCTCGTGTGTGTAGCTTTGGGAAAT
414












TTGGAAATCAGAACGCGTGGCGGATGT


PALB2
NM_024675
6
16
−
78
−
0
37
1
0
CGCGAATGCCACAAATCACTCCTTGGGAATTAC
415












ATACCTGATCTCACGCGTGGCGGATGT


EPHB1
NM_004441
16
3
+
−16
+
0
37
1
0
CGCGAATGCCGGCTCTTTCCTCCTAGAGACCTCC
416












TGAGAATAGGCACGCGTGGCGGATGT


EPHB1
NM_004441
16
3
+
19
−
0
37
1
0
CGCGAATGCCTTCAGGATCTTCTTCTGATGGCCT
417












GCCAAGGTGATACGCGTGGCGGATGT


EPHB1
NM_004441
16
3
+
54
+
0
37
1
0
CGCGAATGCCCAGCATTCATTCTATGAGGGTCC
418












AGATAAGTCAGTACGCGTGGCGGATGT


EPHB1
NM_004441
16
3
+
89
−
0
37
1
0
CGCGAATGCCAAGAAACAAGAGTTCTCATGCCA
419












TTGCCGTTGGTGACGCGTGGCGGATGT


NFKB1
NM_003998
11
4
+
0
+
0
37
1
0
CGCGAATGCCTTTGCCATTGTCTTCAAAACTCCA
420












AAGTATAAAGAACGCGTGGCGGATGT


NFKB1
NM_003998
11
4
+
35
−
0
37
1
0
CGCGAATGCCGGACAAACACAGAGGCTGGTTTT
421












GTAATATTAATAACGCGTGGCGGATGT


NFKB1
NM_003998
11
4
+
70
+
0
37
1
0
CGCGAATGCCAGCTTCGGAGGAAATCTGACTTG
422












GAAACTAGTGAAACGCGTGGCGGATGT


NFKB1
NM_003998
11
4
+
105
−
0
37
1
0
CGCGAATGCCCCTTTGATTTCAGGATAGTAGAG
423












GAAAGGTTTTGGACGCGTGGCGGATGT


PDGFRA
NM_006206
23
4
+
0
+
0
37
1
0
CGCGAATGCCCTCGCAGACCTCTGAAGAGAGTG
424












CCATTGAGACGGACGCGTGGCGGATGT


PDGFRA
NM_006206
23
4
+
35
−
0
37
1
0
CGCGAATGCCGTCCTCTCTCTTGATGAAGGTGG
425












AACTGCTGGAACACGCGTGGCGGATGT


PDGFRA
NM_006206
23
4
+
70
+
0
37
1
0
CGCGAATGCCGAGACCATTGAAGACATCGACAT
426












GATGGATGACATACGCGTGGCGGATGT


PDGFRA
NM_006206
23
4
+
105
−
0
37
1
0
CGCGAATGCCAGCTGTCTTCCACCAGGTCTGAA
427












GAGTCTATGCCGACGCGTGGCGGATGT


PDGFRA
NM_006206
23
4
+
140
+
0
37
1
0
CGCGAATGCCTCCTGTAACTGGCGGATTCGAGG
428












GGTTCCTTCCACACGCGTGGCGGATGT


RB1
NM_000321
20
13
+
0
+
0
37
1
0
CGCGAATGCCTGTATCGGCTAGCCTATCTCCGG
429












CTAAATACACTTACGCGTGGCGGATGT


RB1
NM_000321
20
13
+
35
−
0
37
1
0
CGCGAATGCCTCTAATTCTGGGTGCTCAGACAG
430












AAGGCGTTCACAACGCGTGGCGGATGT


RB1
NM_000321
20
13
+
70
+
0
37
1
0
CGCGAATGCCACATATCATCTGGACCCTTTTCCA
431












GCACACCCTGCACGCGTGGCGGATGT


RB1
NM_000321
20
13
+
105
−
0
37
1
0
CGCGAATGCCCAAATGCCTGTCTCTCATGAGTTC
432












ATACTCATTCTACGCGTGGCGGATGT


RB1
NM_000321
20
13
+
140
+
0
37
1
0
CGCGAATGCCGACCAAGTAAGAAAATCAAGCA
433












CTTCACCTTCTCTACGCGTGGCGGATGT


NFKB1
NM_003998
14
4
+
0
+
0
37
1
0
CGCGAATGCCGAACCATGGACACTGAATCTAAA
434












AAGGACCCTGAAACGCGTGGCGGATGT


NFKB1
NM_003998
14
4
+
35
−
0
37
1
0
CGCGAATGCCTTTACAGTGTTTTTGTCATCACTT
435












TTGTCACAACCACGCGTGGCGGATGT


NFKB1
NM_003998
14
4
+
70
+
0
37
1
0
CGCGAATGCCCCTCTTTGGGAAAGTTATTGAAA
436












CCACAGAGCAAGACGCGTGGCGGATGT


NFKB1
NM_003998
14
4
+
105
−
0
37
1
0
CGCGAATGCCACCATTCCCAACGGTGGCCTCGC
437












TGGGCTCCTGATACGCGTGGCGGATGT


NFKB1
NM_003998
14
4
+
140
+
0
37
1
0
CGCGAATGCCGAGGTCACTCTAACGTATGCAAC
438












AGGAACAAAAGAACGCGTGGCGGATGT


NFKB1
NM_003998
14
4
+
175
−
0
37
1
0
CGCGAATGCCTGTGTGCTCACTTACCCTGAACTC
439












CAGCACTCTCTACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
0
+
0
37
1
0
CGCGAATGCCTTAAAGGAGAAATTAGCATTCTT
440












GAAAAGGGAATAACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
35
−
0
37
1
0
CGCGAATGCCATTCACTTACCTGAAGGCGGGCT
441












AGTGTCTTGCTGACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
70
+
0
37
1
0
CGCGAATGCCCGTATTCTCAAATTAAGGTGTTAT
442












AGTACAAACAAACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
105
−
0
37
1
0
CGCGAATGCCTTTAAAGTTTTATAGAGTCAAGA
443












ACTGTTTTTAAAACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
140
+
0
37
1
0
CGCGAATGCCGAAAACGTATTTCTGGGGCTGTT
444












TTTGTCTCCTCTACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
175
−
0
37
1
0
CGCGAATGCCAGAATGCTTAATCTTTTCAGCTCT
445












TTGGGCACGCTACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
210
+
0
37
1
0
CGCGAATGCCATTAAGAAAACAGTAGAAGAAC
446












AAGATTGTTTGTCACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
245
−
0
37
1
0
CGCGAATGCCCTGAGTGTTTTAGCTGCGGTGAG
447












AGATCCTGCTGAACGCGTGGCGGATGT


PALB2
NM_024675
12
16
−
280
+
0
37
1
0
CGCGAATGCCGTAAATCTAGACCATTCACTTAT
448












GCCTGCTTTATTACGCGTGGCGGATGT


RPS6KA1
NM_002953
20
1
+
−11
+
0
37
1
0
CGCGAATGCCCTTTTCTGCAGATATACTCCATTT
449












GCCAACGGTCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
20
1
+
24
−
0
37
1
0
CGCGAATGCCCGATCCGGGTTAGGATTTCCTCT
450












GGTGTGTCACTGACGCGTGGCGGATGT


RPS6KA1
NM_002953
20
1
+
59
+
0
37
1
0
CGCGAATGCCGCAGTGGGAAGTTTACCCTCAGT
451












GGGGGAAATTGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
20
1
+
94
−
0
37
1
0
CGCGAATGCCTACAGACTCACCTTGGCTGTCTCT
452












GAAACTGTGTTACGCGTGGCGGATGT


CENTG1
NM_014770
17
12
−
−40
+
0
37
1
0
CGCGAATGCCGCCAGGAGCCTCCCCTCTTACTG
453












CCCTTCTCCCGTACGCGTGGCGGATGT


CENTG1
NM_014770
17
12
−
−5
−
0
37
1
0
CGCGAATGCCAAGTCCATTCCTGGCTATTGATC
454












ACAGCCTCTGTAACGCGTGGCGGATGT


CENTG1
NM_014770
17
12
−
30
+
0
37
1
0
CGCGAATGCCTGAGCCGCTCCATTCCTGAACTG
455












CGCCTGGTAGGTACGCGTGGCGGATGT


CENTG1
NM_014770
17
12
−
65
−
0
37
1
0
CGCGAATGCCAGGTCAGCCTTCCCTATAGGCAA
456












GGGGACTGGGTTACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
0
+
0
37
1
0
CGCGAATGCCAAACACGATGCCCAGTTTACTGT
457












CATTCAGCTAGTACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
35
−
0
37
1
0
CGCGAATGCCACTTCATGCCAGATGCTATCCCTC
458












GAAGCATCCCCACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
70
+
0
37
1
0
CGCGAATGCCACCTGTCAGACATGGGCTATGTT
459












CACCGAGACCTCACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
105
−
0
37
1
0
CGCGAATGCCACCAAGTTACTGTTGATCAAGAT
460












GTTCCGAGCAGCACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
140
+
0
37
1
0
CGCGAATGCCGTGTAAGGTTTCTGATTTCGGACT
461












TTCGCGTGTCCACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
175
−
0
37
1
0
CGCGAATGCCTCTTGTTGTATAAGCAGCTTCTGG
462












GTCATCCTCCAACGCGTGGCGGATGT


EPHA3
NM_005233
13
3
+
210
+
0
37
1
0
CGCGAATGCCGTGAGTAACTTAGATTTTCTCCTT
463












TTTTATCATTGACGCGTGGCGGATGT


EPHA3
NM_005233
6
3
+
−8
+
0
37
1
0
CGCGAATGCCCTTTACAGCTCCATCACCTGTCCT
464












GACGATTAAGAACGCGTGGCGGATGT


EPHA3
NM_005233
6
3
+
27
−
0
37
1
0
CGCGAATGCCGGACAAAGAGATGCTATTTCTGG
465












AGGTCCGATCTTACGCGTGGCGGATGT


EPHA3
NM_005233
6
3
+
62
+
0
37
1
0
CGCGAATGCCTGGCAAGAACCTGAACATCCTAA
466












TGGGATCATATTACGCGTGGCGGATGT


EPHA3
NM_005233
6
3
+
97
−
0
37
1
0
CGCGAATGCCTCCCCACCTTTTCATAGTATTTGA
467












CCTCGTAGTCCACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
0
+
0
37
1
0
CGCGAATGCCAAAGGCAAAGGCATCACAATGCT
468












GGAAGAAATCAAACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
35
−
0
37
1
0
CGCGAATGCCCCGTCAAAGTGTACACCAATTTG
469












ATGGATGGGACTACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
70
+
0
37
1
0
CGCGAATGCCTCCCCGAGGCCACGGTGAAAGAC
470












AGTGGAGATTACACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
105
−
0
37
1
0
CGCGAATGCCTTGACCTCCCTGGTAGCCTGGCG
471












GGCAGCACATTCACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
140
+
0
37
1
0
CGCGAATGCCAGAAATGAAGAAAGTCACTATTT
472












CTGTCCATGGTAACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
175
−
0
37
1
0
CGCGAATGCCCAGCATGGACAACTGACATTTTA
473












GAAAGCGGAATGACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
210
+
0
37
1
0
CGCGAATGCCCTCGGGATCCATATGTGGTAATC
474












ATTATTTAATGGACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
245
−
0
37
1
0
CGCGAATGCCTTCAATGAAACCTTTCTCTGTACA
475












GGGAAGAGTTTACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
280
+
0
37
1
0
CGCGAATGCCATCAAACCCACCTTCAGCCAGTT
476












GGAAGCTGTCAAACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
315
−
0
37
1
0
CGCGAATGCCGCACCTCTACAACAAAATGTTTG
477












ACTTCATGCAGGACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
350
+
0
37
1
0
CGCGAATGCCGGGCCTACCCACCTCCCAGGATA
478












TCCTGGCTGAAAACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
385
−
0
37
1
0
CGCGAATGCCATCTCAGTGAGATTTTCAATCAG
479












AGTCAGATTGTTACGCGTGGCGGATGT


PDGFRA
NM_006206
7
4
+
420
+
0
37
1
0
CGCGAATGCCCACCACTGATGTGGAAAAGATTC
480












AGGAAATAAGGTACGCGTGGCGGATGT


RET
NM_020630
14
10
+
0
+
0
37
1
0
CGCGAATGCCGCCCGCTCCTCCTCATCGTGGAG
481












TACGCCAAATACACGCGTGGCGGATGT


RET
NM_020630
14
10
+
35
−
0
37
1
0
CGCGAATGCCTTGCGGCTCTCGCGGAGGAAGCC
482












CCGCAGGGAGCCACGCGTGGCGGATGT


RET
NM_020630
14
10
+
70
+
0
37
1
0
CGCGAATGCCAGTGGGGCCTGGCTACCTGGGCA
483












GTGGAGGCAGCCACGCGTGGCGGATGT


RET
NM_020630
14
10
+
105
−
0
37
1
0
CGCGAATGCCCCGCTCATCCGGGTGGTCCAGGG
484












AGCTGGAGTTGCACGCGTGGCGGATGT


RET
NM_020630
14
10
+
140
+
0
37
1
0
CGCGAATGCCGCCCTCACCATGGGCGACCTCAT
485












CTCATTTGCCTGACGCGTGGCGGATGT


RET
NM_020630
14
10
+
175
−
0
37
1
0
CGCGAATGCCTCTCGGCCAGATACTGCATCCCC
486












TGTGAGATCTGCACGCGTGGCGGATGT


RET
NM_020630
14
10
+
210
+
0
37
1
0
CGCGAATGCCTGAAGGTGCGTGCATATGGCTCT
487












GCACCCAGCCAGACGCGTGGCGGATGT


NFKB1
NM_003998
18
4
+
0
+
0
37
1
0
CGCGAATGCCGTCTGAATGCCATTCATCTAGCC
488












ATGATGAGCAATACGCGTGGCGGATGT


NFKB1
NM_003998
18
4
+
35
−
0
37
1
0
CGCGAATGCCCCAGCGGCCACCAGCAGCAGCAA
489












ACATGGCAGGCTACGCGTGGCGGATGT


NFKB1
NM_003998
18
4
+
70
+
0
37
1
0
CGCGAATGCCGGCTGACGTCAATGCTCAGGAGC
490












AGAAGTCCGGGCACGCGTGGCGGATGT


NFKB1
NM_003998
18
4
+
105
−
0
37
1
0
CGCGAATGCCGTTGTCGTGCTCCACAGCCAGGT
491












GCAGTGCTGTGCACGCGTGGCGGATGT


NFKB1
NM_003998
18
4
+
140
+
0
37
1
0
CGCGAATGCCATCTCATTGGCAGGCTGCCTGCT
492












CCTGGAGGTGAAACGCGTGGCGGATGT


EPHA3
NM_005233
15
3
+
0
+
0
37
1
0
CGCGAATGCCGTAATTAAAGCTGTAGATGAGGG
493












CTATCGACTGCCACGCGTGGCGGATGT


EPHA3
NM_005233
15
3
+
35
−
0
37
1
0
CGCGAATGCCGCTGATACAAGGCAGCTGGGCAG
494












TCCATGGGGGGTACGCGTGGCGGATGT


EPHA3
NM_005233
15
3
+
70
+
0
37
1
0
CGCGAATGCCTGATGCTGGACTGCTGGCAGAAA
495












GACAGGAACAACACGCGTGGCGGATGT


EPHA3
NM_005233
15
3
+
105
−
0
37
1
0
CGCGAATGCCTCCAGAATACTAACAATCTGCTC
496












AAACTTGGGTCTACGCGTGGCGGATGT


EPHA3
NM_005233
15
3
+
140
+
0
37
1
0
CGCGAATGCCCAAGCTTATCCGGAATCCCGGCA
497












GCCTGAAGATCAACGCGTGGCGGATGT


EPHA3
NM_005233
15
3
+
175
−
0
37
1
0
CGCGAATGCCAAATTGAATGTGTCACCTTGCGG
498












CTGCACTGGTGAACGCGTGGCGGATGT


PKN1
NM_002741
4
19
+
−2
+
0
37
1
0
CGCGAATGCCAGGACCGGAAGCTGCTGCTGACA
499












GCCCAGCAGATGACGCGTGGCGGATGT


PKN1
NM_002741
4
19
+
33
−
0
37
1
0
CGCGAATGCCCGGATGATGTCAATCTTGGTCTT
500












ACTGTCCTGCAAACGCGTGGCGGATGT


PKN1
NM_002741
4
19
+
68
+
0
37
1
0
CGCGAATGCCCATGCAACTCCGCCGGGCGCTGC
501












AGGCCGGCCAGCACGCGTGGCGGATGT


PKN1
NM_002741
4
19
+
103
−
0
37
1
0
CGCGAATGCCACCTTGGGTGTCATCCGGGGCTG
502












CCTGGTTCTCCAACGCGTGGCGGATGT


EPHA4
NM_004438
17
2
−
−36
+
0
37
1
0
CGCGAATGCCTTGGAAATTCATCTATTTTTCTTT
503












TGTTTTTTGCAACGCGTGGCGGATGT


EPHA4
NM_004438
17
2
−
−1
−
0
37
1
0
CGCGAATGCCTCTCCCTGAACAGATCTGGAATC
504












CAATAAGGTAACACGCGTGGCGGATGT


EPHA4
NM_004438
17
2
−
34
+
0
37
1
0
CGCGAATGCCACTTGGGTGGATAGCAAGCCCTC
505












TGGAAGGAGGGGACGCGTGGCGGATGT


EPHA4
NM_004438
17
2
−
69
−
0
37
1
0
CGCGAATGCCTTTACCCTTTGGATCAGAGAGCA
506












ACTCAGGACTTAACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
0
+
0
37
1
0
CGCGAATGCCGTTTCAGGAGATGTGTTACAAGG
507












CTTATCTAGCTAACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
35
−
0
37
1
0
CGCGAATGCCGAAAAGATTTATGAAGAGATTGG
508












CATGCTGTCGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
70
+
0
37
1
0
CGCGAATGCCTCAATGATGCTTGGCTCTGGAAT
509












GCCAGAACTACAACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
105
−
0
37
1
0
CGCGAATGCCGGGTCTTTCGAATGTATGCAATG
510












TCATCAAAAGATACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
140
+
0
37
1
0
CGCGAATGCCTAGCCTTAGATAAAACTGAGCAA
511












GAGGCTTTGGAGACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
175
−
0
37
1
0
CGCGAATGCCCCATGATGTGCATCATTCATTTGT
512












TTCATGAAATAACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
210
+
0
37
1
0
CGCGAATGCCTGGCTGGACAACAAAAATGGATT
513












GGATCTTCCACAACGCGTGGCGGATGT


PIK3CA
NM_006218
21
3
+
245
−
0
37
1
0
CGCGAATGCCGTTATCTTTTCAGTTCAATGCATG
514












CTGTTTAATTGACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
0
+
0
37
1
0
CGCGAATGCCGGCAAAGGCACAGAGGAAACCT
515












TCTGGCTGATTGGACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
35
−
0
37
1
0
CGCGAATGCCGGGGCACAGGAAGGGGCTTCATG
516












AAGCCTTTTTTCACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
70
+
0
37
1
0
CGCGAATGCCCACCAGTGGACAAAGATGGGTAA
517












GTGGAGTTCACAACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
105
−
0
37
1
0
CGCGAATGCCGTGGCAGAGTATGGCATAGGAAG
518












ACTCCAATTAAAACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
140
+
0
37
1
0
CGCGAATGCCACTTTCTTCACTACCTATTTGATG
519












TCTCCCCTGCCACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
175
−
0
37
1
0
CGCGAATGCCCCACTGGTTGCAGGCCATGGCCC
520












ACTTGCCTGCAGACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
210
+
0
37
1
0
CGCGAATGCCAGATTGCAGCCTTCCAAAGAAGA
521












AAAGCAGAAAGGACGCGTGGCGGATGT


GUCY2F
NM_001522
3
X
−
245
−
0
37
1
0
CGCGAATGCCTGGCCATTACCTTATGGCTTGTTT
522












CTCACCAACTGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
0
+
0
37
1
0
CGCGAATGCCATTTTCAGATTCTACTTCAAAGAC
523












TCCTCCTCAAGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
35
−
0
37
1
0
CGCGAATGCCAAATACAGGAGATGACACTCGAG
524












TAGGTAATTCTTACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
70
+
0
37
1
0
CGCGAATGCCGGAGCTACCTCTAGTATCAAAAG
525












TGGTTTAGATTTACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
105
−
0
37
1
0
CGCGAATGCCCAGGCTGTAAAAGAGAAGGGGA
526












CAAACTTGTATTCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
140
+
0
37
1
0
CGCGAATGCCGGAAAAAAAAACATCTGAAAAC
527












ACTCCCTTTTAGCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
175
−
0
37
1
0
CGCGAATGCCGATCTAGTTTTTTCTAATCTAGAT
528












ATACAAGTGTTACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
210
+
0
37
1
0
CGCGAATGCCAAAATCTGAAGATAGTGCCCTTT
529












TCACACATCACAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
245
−
0
37
1
0
CGCGAATGCCCTGGATAATGATCTTGTTCACTTC
530












AGACCCAAGACACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
280
+
0
37
1
0
CGCGAATGCCTCATCTAATAAACAGATACTTAT
531












AAATAAAAATATACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
315
−
0
37
1
0
CGCGAATGCCACTCAGTCCTATTCTGTTCACCTA
532












GGGATTCACTTACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
350
+
0
37
1
0
CGCGAATGCCACGGTAAAGATTCTAACACTGAT
533












AAACATTTGGAGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
385
−
0
37
1
0
CGCGAATGCCCTTTTGGATGTTCGGCCTCCCAAT
534












GATTTCAGGGGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
420
+
0
37
1
0
CGCGAATGCCGAAGAAAACTGAGGAAGAAAGT
535












GAACATGAAGTAAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
455
−
0
37
1
0
CGCGAATGCCAGCATTTTCTTTATCAAAAGAAG
536












CTTGGGGGCAGCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
490
+
0
37
1
0
CGCGAATGCCTTCCCTTTTCCAATGGATAATCAG
537












TTTTCCATGAAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
525
−
0
37
1
0
CGCGAATGCCACAGATCCAGAGGTTTATCCATC
538












ACACAGTCTCCAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
560
+
0
37
1
0
CGCGAATGCCCTGATCGATTTTCAGCTATTCAGC
539












GTCAAGAGAAAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
595
−
0
37
1
0
CGCGAATGCCCTAAATTTGTTTTTAGAAGTCTCA
540












CTTCCTTGGCTACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
630
+
0
37
1
0
CGCGAATGCCGCAAGTGACTCTTTATGAGGCTT
541












TGAAGACCATTCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
665
−
0
37
1
0
CGCGAATGCCATCTGAGGCCTTACGGCTTGAGG
542












AAAAGCCCTTTGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
700
+
0
37
1
0
CGCGAATGCCGGCAACTGCACGTTGCCCAAAGA
543












TTCCCCAGGGGAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
735
−
0
37
1
0
CGCGAATGCCAGGGCTGAAGGATGATGCATTCC
544












TGTGAACAGGGCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
770
+
0
37
1
0
CGCGAATGCCTGAATAAATGCTCTCCAGACAAT
545












AAACCATCATTAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
805
−
0
37
1
0
CGCGAATGCCGGAATTTTAAAGACAGCATTTTC
546












TTCTTTTATTTGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
840
+
0
37
1
0
CGCGAATGCCTCTACGTCCACGTGAAAGTTTGG
547












AGACTGAGAATGACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
875
−
0
37
1
0
CGCGAATGCCTGAACATTTAACACAAACCTTTA
548












TGTCATCTAAAAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
910
+
0
37
1
0
CGCGAATGCCAGGATTTTGATTAAAATGATTGC
549












TTGTGATTTCATACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
945
−
0
37
1
0
CGCGAATGCCCAGCACTCTAAAACAGAAATAAT
550












TGTTAGCTCTAAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
980
+
0
37
1
0
CGCGAATGCCGTTCTCATGAGCCAATAAAAATA
551












CAAACCAGGTCAACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
1015
−
0
37
1
0
CGCGAATGCCTGAAGAACTGATGCAAGTTCACA
552












TCCTCCATGGTCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
1050
+
0
37
1
0
CGCGAATGCCGTTAAATCCATGTAGAACTGGTA
553












AAATAAAGTCTCACGCGTGGCGGATGT


RBBP8
NM_002894
12
18
+
1085
−
0
37
1
0
CGCGAATGCCCCTGTTTATGGTGTACACACCTTG
554












GTTGTTTTGTAACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
0
+
0
37
1
0
CGCGAATGCCGCGTCCCATCAGGTCCCCGCAAT
555












GTTATCTCCATCACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
35
−
0
37
1
0
CGCGAATGCCGGGTGCCACTCCAGAATGATGGA
556












CGTCTCATTGACACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
70
+
0
37
1
0
CGCGAATGCCTCCAAGGGAGACAGGTGGGCGG
557












GATGATGTGACCTACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
105
−
0
37
1
0
CGCGAATGCCGCGGTCTGCCCGGCACTTTTTGC
558












AGATGATGTTGTACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
140
+
0
37
1
0
CGCGAATGCCCGGAGCTGCTCCCGCTGTGACGA
559












CAATGTGGAGTTACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
175
−
0
37
1
0
CGCGAATGCCCGCGGCACTCCGTCAGGCCCAGC
560












TGCCTGGGCACAACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
210
+
0
37
1
0
CGCGAATGCCTCTCCATCAGCAGCCTGTGGGCC
561












CACACCCCCTACACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
245
−
0
37
1
0
CGCGAATGCCCTGGAGACTCCATTGATGGCCTG
562












GATGTCAAAGGTACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
280
+
0
37
1
0
CGCGAATGCCCAAGAGTCCCTTCCCCCCACAGC
563












ACGTCTCTGTCAACGCGTGGCGGATGT


EPHB1
NM_004441
5
3
+
315
−
0
37
1
0
CGCGAATGCCGCCTCCAGACTTACCGGCTTGGT
564












TTGTGGTGATGTACGCGTGGCGGATGT


RPS6KA1
NM_002953
9
1
+
0
+
0
37
1
0
CGCGAATGCCACTTTGGCCTGAGCAAAGAGGCC
565












ATTGACCACGAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
9
1
+
35
−
0
37
1
0
CGCGAATGCCTACTCCACTGTCCCGCAGAAAGA
566












ATAGGCCTTCTTACGCGTGGCGGATGT


RPS6KA1
NM_002953
9
1
+
70
+
0
37
1
0
CGCGAATGCCCATGGCCCCTGAGGTCGTCAACC
567












GCCAGGGCCACTACGCGTGGCGGATGT


RPS6KA1
NM_002953
9
1
+
105
−
0
37
1
0
CGCGAATGCCCAACACCCCATAGGACCACCAGT
568












CCGCACTATGGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
9
1
+
140
+
0
37
1
0
CGCGAATGCCATGGTGAGTGCCCAGACAGGGGT
569












AAAGGATCCAGCACGCGTGGCGGATGT


RPS6KA1
NM_001006665
1
1
+
−2
+
0
37
1
0
CGCGAATGCCGGATGGAGCAGGATCCCAAGCCG
570












CCCCGTCTGCGGACGCGTGGCGGATGT


RPS6KA1
NM_001006665
1
1
+
33
−
0
37
1
0
CGCGAATGCCTGCTTCCTGGGAAGCCAGGGGAT
571












CAGGGCCCAGAGACGCGTGGCGGATGT


RPS6KA1
NM_001006665
1
1
+
68
+
0
37
1
0
CGCGAATGCCGCGGCCCAGGATCAGCCAGACCT
572












CTCTGCCTGTCCACGCGTGGCGGATGT


RPS6KA1
NM_001006665
1
1
+
103
−
0
37
1
0
CGCGAATGCCCACCGAGTCCCGCTGGGGGCCAG
573












AGCCAGGGCCAGACGCGTGGCGGATGT


GUCY2F
NM_001522
16
X
−
−28
+
0
37
1
0
CGCGAATGCCTTCTTCCTCTTCCTCTCACTCTCTG
574












CAGGCATCGAACGCGTGGCGGATGT


GUCY2F
NM_001522
16
X
−
7
−
0
37
1
0
CGCGAATGCCGCAAAGTAAGGCAGACCATCATG
575












GCAAAGGCAGGGACGCGTGGCGGATGT


GUCY2F
NM_001522
16
X
−
42
+
0
37
1
0
CGCGAATGCCTTATAGCCCTGCTGTCTATTAATG
576












GATTTGCTTACACGCGTGGCGGATGT


GUCY2F
NM_001522
16
X
−
77
−
0
37
1
0
CGCGAATGCCTATATACAAAAATAACAAAAAAT
577












GTACCTTATAAAACGCGTGGCGGATGT


RBBP8
NM_002894
5
18
+
−14
+
0
37
1
0
CGCGAATGCCGTTTTGTTTCATAGGTTAAGAGC
578












AGGCTTATGTGAACGCGTGGCGGATGT


RBBP8
NM_002894
5
18
+
21
−
0
37
1
0
CGCGAATGCCTTTTCCGCATATGTTCTTCAGTTA
579












CTGCACAGCGAACGCGTGGCGGATGT


RBBP8
NM_002894
5
18
+
56
+
0
37
1
0
CGCGAATGCCAACAGCAAGAGTTTGAAAATATC
580












CGGCAGCAGAATACGCGTGGCGGATGT


RBBP8
NM_002894
5
18
+
91
−
0
37
1
0
CGCGAATGCCAAAGGAAACTCACTAAGTTCTGT
581












AATAAGTTTAAGACGCGTGGCGGATGT


KSR2
NM_173598
3
12
−
−5
+
0
37
1
0
CGCGAATGCCAACAGCACAATCTGGTATGAACT
582












CCACGCCAGGGAACGCGTGGCGGATGT


KSR2
NM_173598
3
12
−
30
−
0
37
1
0
CGCGAATGCCTTATTGCCTCTGCTGGTTGGGTCT
583












TGAAAGGCCATACGCGTGGCGGATGT


KSR2
NM_173598
3
12
−
65
+
0
37
1
0
CGCGAATGCCTCTGGCAAATGGGCACAGGCATG
584












AAACCCAACCTCACGCGTGGCGGATGT


KSR2
NM_173598
3
12
−
100
−
0
37
1
0
CGCGAATGCCCTTACCGAGATTTCTTTTCCCATG
585












CCAATCTGGCTACGCGTGGCGGATGT


EPHA7
NM_004440
2
6
−
0
+
0
37
1
0
CGCGAATGCCGCCAATAAGCCCTCTTCTGGATC
586












AAAACACTCCTGACGCGTGGCGGATGT


EPHA7
NM_004440
2
6
−
35
−
0
37
1
0
CGCGAATGCCTAGCCATTCTCCAACTGAACAAA
587












AGGTAGTGAAATACGCGTGGCGGATGT


EPHA7
NM_004440
2
6
−
70
+
0
37
1
0
CGCGAATGCCCAAGCTATTAAGATGGAAAGATA
588












TAAAGATAATTTACGCGTGGCGGATGT


EPHA7
NM_004440
2
6
−
105
−
0
37
1
0
CGCGAATGCCCTACTGATTCAAGGGAATTGTAG
589












CCAGCTGCCGTGACGCGTGGCGGATGT


EPHA7
NM_004440
2
6
−
140
+
0
37
1
0
CGCGAATGCCCCAGGATGACTATTGAGTAAGCT
590












TAAACTCTTAAAACGCGTGGCGGATGT


RB1
NM_000321
24
13
+
−54
+
0
37
1
0
CGCGAATGCCCAAAATTGTATATGGTTTTTTATT
591












ACTAATTGGTAACGCGTGGCGGATGT


RB1
NM_000321
24
13
+
−19
−
0
37
1
0
CGCGAATGCCAATTGATACTAAGATTCTGTCAA
592












GTTAAGATGAAAACGCGTGGCGGATGT


RB1
NM_000321
24
13
+
16
+
0
37
1
0
CGCGAATGCCGGTGAATCATTCGGGGTGAGTAT
593












TTTCTTTCTATGACGCGTGGCGGATGT


RB1
NM_000321
24
13
+
51
−
0
37
1
0
CGCGAATGCCTTTCTTTTATACTTACAATGCATA
594












CTATTATATTTACGCGTGGCGGATGT


EPHA4
NM_004438
11
2
−
−14
+
0
37
1
0
CGCGAATGCCATTGTCATTCCCAGTGCCTTCCCG
595












GATCATTGGAGACGCGTGGCGGATGT


EPHA4
NM_004438
11
2
−
21
−
0
37
1
0
CGCGAATGCCGACAGAGACCAGAAGGACTGTG
596












GAGTTAGCCCCATACGCGTGGCGGATGT


EPHA4
NM_004438
11
2
−
56
+
0
37
1
0
CGCGAATGCCTCGGGCAGTGTGGTGCTGGTGGT
597












AATTCTCATTGCACGCGTGGCGGATGT


EPHA4
NM_004438
11
2
−
91
−
0
37
1
0
CGCGAATGCCGCTTCAGTGCTTACCTCCGGCTG
598












ATGACAAAAGCTACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
0
+
0
37
1
0
CGCGAATGCCCAAAATGACGGGCAGTTCACCGT
599












GATCCAGCTTGTACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
35
−
0
37
1
0
CGCGAATGCCACTTCATGCCAGCAGCGATGCCC
600












CTGAGCATACCCACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
70
+
0
37
1
0
CGCGAATGCCACCTGGCTGAGATGAATTATGTG
601












CATCGGGACCTGACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
105
−
0
37
1
0
CGCGAATGCCACCAGGTTACTGTTGACCAGAAT
602












GTTCCTAGCAGCACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
140
+
0
37
1
0
CGCGAATGCCGTGCAAGGTGTCCGACTTTGGCC
603












TCTCCCGCTACCACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
175
−
0
37
1
0
CGCGAATGCCGCTGGTGTAGGTGGGATCTGAGG
604












TGTCATCCTGGAACGCGTGGCGGATGT


EPHB1
NM_004441
12
3
+
210
+
0
37
1
0
CGCGAATGCCTCCTTGGTGAGTCCTTCTTGGCAT
605












TCTCAAGTAGAACGCGTGGCGGATGT


RB1
NM_000321
26
13
+
−45
+
0
37
1
0
CGCGAATGCCATTTATAAATACACATGAAATGT
606












TTTGCATTTTTTACGCGTGGCGGATGT


RB1
NM_000321
26
13
+
−10
−
0
37
1
0
CGCGAATGCCTTTGGACTCTCCTGGGAGATGTTT
607












ACTGCAGATTAACGCGTGGCGGATGT


RB1
NM_000321
26
13
+
25
+
0
37
1
0
CGCGAATGCCTTTCAGCAGAAACTGGCAGAAAT
608












GAGTAAGTACTTACGCGTGGCGGATGT


RB1
NM_000321
26
13
+
60
−
0
37
1
0
CGCGAATGCCAAACAATTGTTTATTTCATTTACA
609












CAAGGTGAAAAACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
0
+
0
37
1
0
CGCGAATGCCGAGTGTGCCGACCCTGAGAACCA
610












TAAGGTCCGCCAACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
35
−
0
37
1
0
CGCGAATGCCCCAGGAACTCGTCCCACTCCTTG
611












GCCTTCAGTTTCACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
70
+
0
37
1
0
CGCGAATGCCCTAAGGGGAAGCGCTTTCGCGTC
612












CTGCAACCTGTGACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
105
−
0
37
1
0
CGCGAATGCCCAGTCTCTGTCAGCCGCCCACAC
613












GCAGCCGATCTTACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
140
+
0
37
1
0
CGCGAATGCCCGCAGGCGATGACCTGAAGGTAC
614












TGCAGCAGTTCGACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
175
−
0
37
1
0
CGCGAATGCCCTCCTGGGCCGGCAGGGTCTCCA
615












GGAAGCAGGCTGACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
210
+
0
37
1
0
CGCGAATGCCGAGCAGACGCCCAAGGCCTCCAA
616












GCGGGAGAGGAGACGCGTGGCGGATGT


CHAF1A
NM_005483
12
19
+
245
−
0
37
1
0
CGCGAATGCCTGGCCTGGCCCCGCCCACACTCA
617












CTCTGCTCGTCTACGCGTGGCGGATGT


EPHA3
NM_005233
10
3
+
−7
+
0
37
1
0
CGCGAATGCCCAAACAGTAAAACTTCCAGGTCT
618












CAGGACTTATGTACGCGTGGCGGATGT


EPHA3
NM_005233
10
3
+
28
−
0
37
1
0
CGCGAATGCCCAGCTTGGGTAGGGTCTTCATAT
619












GTATGTGGGTCAACGCGTGGCGGATGT


EPHA3
NM_005233
10
3
+
63
+
0
37
1
0
CGCGAATGCCTTCATGAGTTTGCCAAGGAATTG
620












GATGCCACCAACACGCGTGGCGGATGT


EPHA3
NM_005233
10
3
+
98
−
0
37
1
0
CGCGAATGCCTGGTTACCTGCTCCAACAACTTTA
621












TCAATGGATATACGCGTGGCGGATGT


EPHA3
NM_005233
4
3
+
0
+
0
37
1
0
CGCGAATGCCCTTGTCGACCAGGTTTCTACAAG
622












GCATTGGATGGTACGCGTGGCGGATGT


EPHA3
NM_005233
4
3
+
35
−
0
37
1
0
CGCGAATGCCGAACTGTGAGGCGGGCACTTAGC
623












ACACTTCATATTACGCGTGGCGGATGT


EPHA3
NM_005233
4
3
+
70
+
0
37
1
0
CGCGAATGCCTACTCAGGAAGATGGTTCAATGA
624












ACTGCAGGTGTGACGCGTGGCGGATGT


EPHA3
NM_005233
4
3
+
105
−
0
37
1
0
CGCGAATGCCTGGAGGGTCTTTGTCTGCCCGGA
625












AGTAATTATTCTACGCGTGGCGGATGT


EPHA3
NM_005233
4
3
+
140
+
0
37
1
0
CGCGAATGCCTCCATGGCTTGTACCCGTGAGTA
626












GTTTTGCTGCAAACGCGTGGCGGATGT


RB1
NM_000321
10
13
+
−15
+
0
37
1
0
CGCGAATGCCCTTTTTTCTTTCAAGGTTGAAAAT
627












CTTTCTAAACGACGCGTGGCGGATGT


RB1
NM_000321
10
13
+
20
−
0
37
1
0
CGCGAATGCCCTAGATCTTTATTTTTAAGATAAA
628












TTTCTTCGTATACGCGTGGCGGATGT


RB1
NM_000321
10
13
+
55
+
0
37
1
0
CGCGAATGCCATGCAAGATTATTTTTGGATCAT
629












GATAAAACTCTTACGCGTGGCGGATGT


RB1
NM_000321
10
13
+
90
−
0
37
1
0
CGCGAATGCCTACCATGTGCAATACCTGTCTAT
630












AGAATCAGTCTGACGCGTGGCGGATGT


PIK3CA
NM_006218
15
3
+
−16
+
0
37
1
0
CGCGAATGCCCTTTTTTTTTAATCAGGTACAGAT
631












GAAGTTTTTAGACGCGTGGCGGATGT


PIK3CA
NM_006218
15
3
+
19
−
0
37
1
0
CGCGAATGCCAGCATCCATGAAATCTGGTCGCC
632












TCATTTGCTCAAACGCGTGGCGGATGT


PIK3CA
NM_006218
15
3
+
54
+
0
37
1
0
CGCGAATGCCCTACAGGGCTTTCTGTCTCCTCTA
633












AACCCTGCTCAACGCGTGGCGGATGT


PIK3CA
NM_006218
15
3
+
89
−
0
37
1
0
CGCGAATGCCAACCCCCAAGAAAGTACCTGAGG
634












TTTCCTAGTTGAACGCGTGGCGGATGT


EPHB1
NM_004441
8
3
+
−16
+
0
37
1
0
CGCGAATGCCCCTCTTTCTCACCTAGATGATTAC
635












AAGTCAGAGCTACGCGTGGCGGATGT


EPHB1
NM_004441
8
3
+
19
−
0
37
1
0
CGCGAATGCCCTGCCGAGCCAGCAATCAGGGGC
636












AGCTGCTCCCTCACGCGTGGCGGATGT


EPHB1
NM_004441
8
3
+
54
+
0
37
1
0
CGCGAATGCCCGGCCGGGGTCGTGTTCGTTGTG
637












TCCTTGGTGGCCACGCGTGGCGGATGT


EPHB1
NM_004441
8
3
+
89
−
0
37
1
0
CGCGAATGCCGAGTGGAGGACCTACCTGCTACA
638












GACGATAGAGATACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
0
+
0
37
1
0
CGCGAATGCCTGGGAAGAATTAGTGGTTTGGA
639












TGAGAACTATACACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
35
−
0
37
1
0
CGCGAATGCCCCATGACTTGGCACACCTGGTAT
640












GTTCGTATCGGGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
70
+
0
37
1
0
CGCGAATGCCAGCCCAACCAAAACAACTGGCTG
641












CGGACTAACTGGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
105
−
0
37
1
0
CGCGAATGCCTCTACAAAAATCCTTTGTGCATTG
642












CCTTTGGAAATACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
140
+
0
37
1
0
CGCGAATGCCATTGAAATTCACCCTGAGGGATT
643












GTAACAGTCTTCACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
175
−
0
37
1
0
CGCGAATGCCATTAAATGTTTCCTTGCAAGTTCC
644












CAGTACTCCAGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
210
+
0
37
1
0
CGCGAATGCCTTGTACTATTATGAAACAGACTA
645












TGACACTGGCAGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
245
−
0
37
1
0
CGCGAATGCCTGTCTATTTTTACATAGAGGTTTT
646












CTCTTATATTCACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
280
+
0
37
1
0
CGCGAATGCCCCATTGCTGCAGATGAAAGTTTT
647












ACCCAAGGTGACACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
315
−
0
37
1
0
CGCGAATGCCACCTCAGTGTTAAGCTTCATCTTT
648












CTTTCACCAAGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
350
+
0
37
1
0
CGCGAATGCCGAGAGAGATTGGACCTTTGTCCA
649












AAAAGGGATTCTACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
385
−
0
37
1
0
CGCGAATGCCAGCTATGCAAGCCCCTACATCCT
650












GAAAGGCAAGATACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
420
+
0
37
1
0
CGCGAATGCCTTGGTTTCTGTCAAAGTGTACTAC
651












AAGAAGTGCTGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
455
−
0
37
1
0
CGCGAATGCCTATCTGGAAAGATAGCTAAGTTC
652












TCAATAATGGACACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
490
+
0
37
1
0
CGCGAATGCCCAGTGACTGGTTCAGAATTTTCCT
653












CTTTAGTCGAGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
525
−
0
37
1
0
CGCGAATGCCTCTTCCTCTGCACTGCTGACACAT
654












GTCCCTCGAACACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
560
+
0
37
1
0
CGCGAATGCCAGCGGAAAACGCCCCCAGGATGC
655












ACTGCAGTGCAGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
595
−
0
37
1
0
CGCGAATGCCGATACATTTTCCAATGGGCACTA
656












ACCATTCTCCTTACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
630
+
0
37
1
0
CGCGAATGCCTGCAAAGCAGGCTACCAGCAAAA
657












AGGAGACACTTGACGCGTGGCGGATGT


EPHA7
NM_004440
15
6
−
665
−
0
37
1
0
CGCGAATGCCAGTGGAGTTTTAATAGGACACAT
658












TACTTACGTTCAACGCGTGGCGGATGT


PALB2
NM_024675
8
16
−
−34
+
0
37
1
0
CGCGAATGCCGTGTAGACTAATGATGTGACTTT
659












TGTTTTCACAGAACGCGTGGCGGATGT


PALB2
NM_024675
8
16
−
1
−
0
37
1
0
CGCGAATGCCTATGCTATCAGAAGCAGGAAGCT
660












CTGCTGTTTCAGACGCGTGGCGGATGT


PALB2
NM_024675
8
16
−
36
+
0
37
1
0
CGCGAATGCCAACCCAGGCAACCTACAATTGGT
661












TTCAGAGTTAAAACGCGTGGCGGATGT


PALB2
NM_024675
8
16
−
71
−
0
37
1
0
CGCGAATGCCGACACGAGACACTGGAAGAGAA
662












TATTCTTCTGACCACGCGTGGCGGATGT


RET
NM_020630
3
10
+
0
+
0
37
1
0
CGCGAATGCCACCGCGGCTTTCCCCTGCTCACC
663












GTCTACCTCAAGACGCGTGGCGGATGT


RET
NM_020630
3
10
+
35
−
0
37
1
0
CGCGAATGCCTCGCCCTCACGAAGGGATGTGGG
664












TGACAGGAAGACACGCGTGGCGGATGT


RET
NM_020630
3
10
+
70
+
0
37
1
0
CGCGAATGCCGTGCCAGTGGCCAGGCTGTGCCC
665












GCGTATACTTCTACGCGTGGCGGATGT


RET
NM_020630
3
10
+
105
−
0
37
1
0
CGCGAATGCCGGAGCTGCAGGCTGGAAAGGAG
666












GTGTTGAAGAAGGACGCGTGGCGGATGT


RET
NM_020630
3
10
+
140
+
0
37
1
0
CGCGAATGCCCTCAAGCCCCGGGAGCTCTGCTT
667












CCCAGAGACAAGACGCGTGGCGGATGT


RET
NM_020630
3
10
+
175
−
0
37
1
0
CGCGAATGCCCTGGGGGTCGGTTCTCCCGAATG
668












CGGAAGGAGGGCACGCGTGGCGGATGT


RET
NM_020630
3
10
+
210
+
0
37
1
0
CGCGAATGCCGCACCTTCCACCAGTTCCGCCTG
669












CTGCCTGTGCAGACGCGTGGCGGATGT


RET
NM_020630
3
10
+
245
−
0
37
1
0
CGCGAATGCCAGCCTGTAGGCCACGCTGATGTT
670












GGGGCACAAGAAACGCGTGGCGGATGT


RET
NM_020630
3
10
+
280
+
0
37
1
0
CGCGAATGCCCCTGGAGGGTGAGTGCCGACCTT
671












GTGGGGCCGCCCACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
0
+
0
37
1
0
CGCGAATGCCGGCCCATGGGCCAGATGCAATGA
672












TCCTTGTGGATGACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
35
−
0
37
1
0
CGCGAATGCCGAGCCCCAGCTCACCCTTGGCCT
673












GGCGTGGCTGTCACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
70
+
0
37
1
0
CGCGAATGCCTCCCAAATGCTCCACATTGCCAG
674












TCAGATCGCCTCACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
105
−
0
37
1
0
CGCGAATGCCGCACAAAGTGCTGGGAGGCCAG
675












GTACACCATACCCACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
140
+
0
37
1
0
CGCGAATGCCACCGAGACCTGGCCACCAGGAAC
676












TGCCTGGTTGGAACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
175
−
0
37
1
0
CGCGAATGCCATGCCGAAGTCCCCAATCTTCAC
677












TAGCAGATTCGCACGCGTGGCGGATGT


NTRK3
NM_001012338
4
15
−
210
+
0
37
1
0
CGCGAATGCCGTCCAGAGATGTCTACAGCACGG
678












ATTATTACAGGGACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
0
+
0
37
1
0
CGCGAATGCCGGAAGAAAAGTCGTCATCAAAA
679












AGAGGATTCCCTTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
35
−
0
37
1
0
CGCGAATGCCTCATCCAAGGATAAATAAGCACT
680












ATTACTCCAAGAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
70
+
0
37
1
0
CGCGAATGCCTGATGCTTTCACGGCTCCATTTCA
681












TAGGGATGGAAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
105
−
0
37
1
0
CGCGAATGCCACTGAGAAAAGACAGTAGTTGCT
682












TTAAACTCAGCAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
140
+
0
37
1
0
CGCGAATGCCATCACAGACTTTCAGTTACCTGA
683












TGAAGACTTTGGACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
175
−
0
37
1
0
CGCGAATGCCCTGAGCAGGACTTCACTTTTTCA
684












AGCTTAAGAGGTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
210
+
0
37
1
0
CGCGAATGCCAAAAACCAGTGGAGCCCTTTGAG
685












TCAAAAATGTTTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
245
−
0
37
1
0
CGCGAATGCCAAAATACAGCTTCCCTCTTTAAG
686












ATGTCTCTCTCCACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
280
+
0
37
1
0
CGCGAATGCCTCCAGAGGAACTGAGTCCTAAAC
687












GCATGGATACAGACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
315
−
0
37
1
0
CGCGAATGCCTAGAACAATAAGGTCCTCTTCTA
688












AGTCCTCCATTTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
350
+
0
37
1
0
CGCGAATGCCCCAGGAAAATCACATCCCAAAAG
689












GCCAAACTCGCAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
385
−
0
37
1
0
CGCGAATGCCTGGATGAAGAAAGGCCCGTCTTT
690












GTATGCTGGCTTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
420
+
0
37
1
0
CGCGAATGCCTATTACTTTATACTCCTTTAAATA
691












CGGTTGCGCCTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
455
−
0
37
1
0
CGCGAATGCCGAACACATGTCTGTGGTAGGCCT
692












GTCATTATCATCACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
490
+
0
37
1
0
CGCGAATGCCACCTGCTTTCCCCATCTTAGGTAC
693












TACTCCAGCCTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
525
−
0
37
1
0
CGCGAATGCCTGTAGATGCTTTTTCATAGGAGC
694












CTTGAGGGCCAAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
560
+
0
37
1
0
CGCGAATGCCGAAGTTGCTGGACGAACTTGCTG
695












CACACCCCAACTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
595
−
0
37
1
0
CGCGAATGCCCACTGGCAAGACAGACTGAGTCT
696












TTCAAATGAGCAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
630
+
0
37
1
0
CGCGAATGCCATACTAAACAATTCGACAGTTCA
697












GGCAGCCCAGCAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
665
−
0
37
1
0
CGCGAATGCCTGCCTGCCTGACACTTGCAGGGT
698












GGTATGTGGTTTACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
700
+
0
37
1
0
CGCGAATGCCAGGACAACCTACCTGTGACTGTG
699












ACTCTGTCCCGCACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
735
−
0
37
1
0
CGCGAATGCCAAAAGTGAATGACTCAATGGGTG
700












GAGGTGTTCCTGACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
770
+
0
37
1
0
CGCGAATGCCAAAGAAAATCAGCTCTGTAGAAA
701












CACATGCCAGGAACGCGTGGCGGATGT


PALB2
NM_024675
9
16
−
805
−
0
37
1
0
CGCGAATGCCTGGATTGTACCTGTTCGACGGAA
702












TGTTTATGCAGCACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
0
+
0
37
1
0
CGCGAATGCCGTCACCTTCCGCAACCCTGTCATT
703












GAGAGGATTCCACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
35
−
0
37
1
0
CGCGAATGCCGCTTGGAGAAAATTTTCTTCTGCC
704












GTCGGAGCCGAACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
70
+
0
37
1
0
CGCGAATGCCAGCAAGGTGAGAGGGTGCTCCAG
705












GCTTCCTGGGGGACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
105
−
0
37
1
0
CGCGAATGCCCACCCTCCATGACACTCCCAGGA
706












CCCCTGGCCTCTACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
140
+
0
37
1
0
CGCGAATGCCACGCCTGCCCCGTCCCCCCAGGG
707












AAGGCGTTCCAGACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
175
−
0
37
1
0
CGCGAATGCCCACGTGGCGACATCGATGTTCAT
708












CTGCCTAGCACGACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
210
+
0
37
1
0
CGCGAATGCCGGTGCGGCTGCTCCGGAGGCTCA
709












TCCCCAATGCCAACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
245
−
0
37
1
0
CGCGAATGCCTGGAGAAGCCCCAGGGCTAAAG
710












GTGCCTGTGCCCGACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
280
+
0
37
1
0
CGCGAATGCCGGATCCGAGGCCCGGACCACGGG
711












GTAAGGAAGGAGACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
315
−
0
37
1
0
CGCGAATGCCCGCAGGCTTCAAGGGGCAGCCGG
712












GACCATGGGGCCACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
350
+
0
37
1
0
CGCGAATGCCAGGGTCCTGGGTCCCAGACACAC
713












CCTCCTCTCGTCACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
385
−
0
37
1
0
CGCGAATGCCAGGTTCAGCTTCTCCACCGATAT
714












GTCACTGCAGCAACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
420
+
0
37
1
0
CGCGAATGCCCGGCACTGACTCGGACAGCTCAC
715












CTCAGAAGAGCTACGCGTGGCGGATGT


PKN1
NM_002741
12
19
+
455
−
0
37
1
0
CGCGAATGCCCACCAGGCTCGATGGGCTGGAAG
716












GAGGATCCCGCGACGCGTGGCGGATGT


NTRK3
NM_001012338
7
15
−
0
+
0
37
1
0
CGCGAATGCCGTCCCGTGGCTGTCATCAGTGGT
717












GAGGAGGACTCAACGCGTGGCGGATGT


NTRK3
NM_001012338
7
15
−
35
−
0
37
1
0
CGCGAATGCCGTGATGCCGTGGTTGATGTGGTG
718












CAGTGGGCTGGCACGCGTGGCGGATGT


NTRK3
NM_001012338
7
15
−
70
+
0
37
1
0
CGCGAATGCCCACGCCCTCGTCACTGGATGCCG
719












GGCCCGACACTGACGCGTGGCGGATGT


NTRK3
NM_001012338
7
15
−
105
−
0
37
1
0
CGCGAATGCCCTCAATGACAGGGATGCGAGTCA
720












TGCCAATGACCAACGCGTGGCGGATGT


NTRK3
NM_001012338
7
15
−
140
+
0
37
1
0
CGCGAATGCCAACCCCCAGTACTTCCGTCAGGG
721












ACACAACTGCCAACGCGTGGCGGATGT


NTRK3
NM_001012338
7
15
−
175
−
0
37
1
0
CGCGAATGCCGCATTCCATCCCAGTACTTACAC
722












GTGTCCGGCTTGACGCGTGGCGGATGT


RPS6KA1
NM_002953
14
1
+
−4
+
0
37
1
0
CGCGAATGCCACAGATTCCCCAGGCATCCCCCC
723












CAGCGCTGGGGCACGCGTGGCGGATGT


RPS6KA1
NM_002953
14
1
+
31
−
0
37
1
0
CGCGAATGCCTGGCCACGAAGCTGAAGCCCCGG
724












AACAGCTGATGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
14
1
+
66
+
0
37
1
0
CGCGAATGCCCCGGCCTGATGGAAGACGACGGC
725












AAGCCTCGTGCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
14
1
+
101
−
0
37
1
0
CGCGAATGCCCTCACCTGTACCACCGAGTGCAG
726












GGGTGCCTGCGGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
0
+
0
37
1
0
CGCGAATGCCATGTTCCTGGGACTCGGGCGCTT
727












TTCTCGCCTTGTACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
35
−
0
37
1
0
CGCGAATGCCGTCCCAGCAGTTTCCTGAAAGCC
728












GCAAACCAGAGAACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
70
+
0
37
1
0
CGCGAATGCCACCATGGCCTTGCATCTGCCAAG
729












TTCCTGTGGTGCACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
105
−
0
37
1
0
CGCGAATGCCTGCTGCGGAAGGGACATGACAGA
730












CAGAAGGCACAAACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
140
+
0
37
1
0
CGCGAATGCCGGTGTGGACACTCCCCTACAAGA
731












TAGGGGTGGTGGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
175
−
0
37
1
0
CGCGAATGCCGGCCTTTGAAAACAGCGAATCAC
732












AAGCCCAAGGGCACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
210
+
0
37
1
0
CGCGAATGCCCTGCCTGAGGTTGCTGCGCGATT
733












AGCCATTGAGCGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
245
−
0
37
1
0
CGCGAATGCCAATAACTCAGGTCAAAAGATGGG
734












TCCCGGTTGATTACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
280
+
0
37
1
0
CGCGAATGCCCTTTTGAATACGTGATTCTCAATG
735












AAGACTGCCAGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
315
−
0
37
1
0
CGCGAATGCCTGGTGGGAAATGAAACTGGAGA
736












GAGCCCTCGAAGTACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
350
+
0
37
1
0
CGCGAATGCCCCAGATGGCCTCAGGATTTATTG
737












GACCTACCAACCACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
385
−
0
37
1
0
CGCGAATGCCGTTTCCCAGGAGCGAGGCTGCCT
738












CGCAGTAGCCAGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
420
+
0
37
1
0
CGCGAATGCCAGCTGGGACAAAGGAATTTTCTC
739












TTGGGCTTGTGTACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
455
−
0
37
1
0
CGCGAATGCCTCGGGTAGCTAATTTTATTGTCTA
740












ATTCATAATTCACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
490
+
0
37
1
0
CGCGAATGCCCCTTTTCTCGGACACTCCCTTCTC
741












CCATCCGGGTGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
525
−
0
37
1
0
CGCGAATGCCTGAGCCCACTGGAAATATTTCAT
742












GACAGTTACAAGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
560
+
0
37
1
0
CGCGAATGCCTGCTGGAGTCATTTCCTCAGATG
743












AAGACATTTGGGACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
595
−
0
37
1
0
CGCGAATGCCCCGAAGAGCACTTGCGACTCGAT
744












TGGCTGTATGCAACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
630
+
0
37
1
0
CGCGAATGCCAGCCACGGCTTACCTGTAGGGGT
745












CGTCCTGACCACACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
665
−
0
37
1
0
CGCGAATGCCGGAGGGCTTTCCGCATGCTTTGG
746












CTGTCTTGTCCTACGCGTGGCGGATGT


GUCY2F
NM_001522
19
X
−
700
+
0
37
1
0
CGCGAATGCCAGAGGATTCACCAGGCAGACAG
747












AATTCGCAGTGAGACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
0
+
0
37
1
0
CGCGAATGCCGCTAGTAACGGGGGCCACACTAG
748












CGACTACTCTTCACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
35
−
0
37
1
0
CGCGAATGCCCCCGGTGACCAACATTCGGTGAG
749












GACGGGAGGGAAACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
70
+
0
37
1
0
CGCGAATGCCAGCTCCGAGCCGAGGCAGCTGCA
750












GTGGCTGGATTGACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
105
−
0
37
1
0
CGCGAATGCCCGCTTGGCTGCCCGGTGCAGGGA
751












CCCTGGGGTGCTACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
140
+
0
37
1
0
CGCGAATGCCCAGGACCAGCCTTTTTGCGGTAT
752












TGGACATGGACAACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
175
−
0
37
1
0
CGCGAATGCCGCAGGTCATGGGGGACAGGAGG
753












ATGGGGGTCAGCAACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
210
+
0
37
1
0
CGCGAATGCCCCATCTTCCCTAGTCCCTAGACTC
754












CTCTGTTGGGCACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
245
−
0
37
1
0
CGCGAATGCCACTGATGTGACGATAGGGAAGAT
755












AGATGAAGAAGGACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
280
+
0
37
1
0
CGCGAATGCCGTATCCCTCTATCCATCCTCAAAC
756












TGATTCCAATAACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
315
−
0
37
1
0
CGCGAATGCCTCTCGGAGTCACTACCCCGACGA
757












TTCTGGAATGGTACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
350
+
0
37
1
0
CGCGAATGCCAACGAAGCTTGGATAGTCGGGGA
758












GAGACAACAGGGACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
385
−
0
37
1
0
CGCGAATGCCTCACTCACCTGTTTGATGGGGAT
759












GGCTCGCCCACTACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
420
+
0
37
1
0
CGCGAATGCCTCCAGGGGCTGGGACAGGCTAGG
760












GGCAGCTGGTCTACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
455
−
0
37
1
0
CGCGAATGCCAAGGGGAAAGACACATGTGGGA
761












AAAAAGCCAACTAACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
490
+
0
37
1
0
CGCGAATGCCTCTCCAGAGCTTCCTACTAAAAC
762












GAAGTGGCAATTACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
525
−
0
37
1
0
CGCGAATGCCGGTTACATATTTCTTCTTCCATTC
763












TTTGTTCAAGGACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
560
+
0
37
1
0
CGCGAATGCCCTGTCCAGTAATGGCTTTCTACTC
764












TACCACCCCAGACGCGTGGCGGATGT


CENTG1
NM_014770
11
12
−
595
−
0
37
1
0
CGCGAATGCCCCAATGTGGCCCAGTCTCTGCCA
765












CTCACGTTAATAACGCGTGGCGGATGT


RBBP8
NM_002894
8
18
+
−18
+
0
37
1
0
CGCGAATGCCATTTTTTTCTCCCCTTAGAAATGA
766












GAAAAGTTTCCACGCGTGGCGGATGT


RBBP8
NM_002894
8
18
+
17
−
0
37
1
0
CGCGAATGCCTCATTAGGATTATGTTGTGGATG
767












AGTTGAAGACTTACGCGTGGCGGATGT


RBBP8
NM_002894
8
18
+
52
+
0
37
1
0
CGCGAATGCCAAATGAAATTCTAGTAGCTGACA
768












CTTATGACCAAAACGCGTGGCGGATGT


RBBP8
NM_002894
8
18
+
87
−
0
37
1
0
CGCGAATGCCCTCAGTATCTTGCTTACTGGCCAT
769












TGGAGATTGACACGCGTGGCGGATGT


NFKB1
NM_003998
19
4
+
−18
+
0
37
1
0
CGCGAATGCCGTGTGGGCTGGATTGTAGGGTGA
770












TGCCCATGTGGAACGCGTGGCGGATGT


NFKB1
NM_003998
19
4
+
17
−
0
37
1
0
CGCGAATGCCTATGCAGGGGTGTGGTTCCATCG
771












TAGGTAGTACTGACGCGTGGCGGATGT


NFKB1
NM_003998
19
4
+
52
+
0
37
1
0
CGCGAATGCCTAGCAGCTGGGAGAGGGTCCACC
772












AGGCTGGCAGCTACGCGTGGCGGATGT


NFKB1
NM_003998
19
4
+
87
−
0
37
1
0
CGCGAATGCCCGCCAGATCACCATCTTACCTGC
773












TGCTTTGAGAAGACGCGTGGCGGATGT


GUCY2F
NM_001522
6
X
−
0
+
0
37
1
0
CGCGAATGCCAGACCATTGGAGATGCCTACATG
774












GTGGCTTCAGGCACGCGTGGCGGATGT


GUCY2F
NM_001522
6
X
−
35
−
0
37
1
0
CGCGAATGCCTCAGCTGCATGCCTACTGCCATTC
775












CTCTTTGGGAGACGCGTGGCGGATGT


GUCY2F
NM_001522
6
X
−
70
+
0
37
1
0
CGCGAATGCCGATTGCAAACATGTCCTTAGATA
776












TCCTGAGCTCTGACGCGTGGCGGATGT


GUCY2F
NM_001522
6
X
−
105
−
0
37
1
0
CGCGAATGCCCACTTCTGGCATGTGCCGCATCTT
777












GAAAGTGCCCAACGCGTGGCGGATGT


GUCY2F
NM_001522
6
X
−
140
+
0
37
1
0
CGCGAATGCCCCGGTCCGAATTCGAATTGGCCT
778












TCACTCAGGTAAACGCGTGGCGGATGT


RB1
NM_000321
3
13
+
−12
+
0
37
1
0
CGCGAATGCCTTTTGTTCCCAGGGAGGTTATATT
779












CAAAAGAAAAAACGCGTGGCGGATGT


RB1
NM_000321
3
13
+
23
−
0
37
1
0
CGCGAATGCCCTGCTGCAATAAAGATACAGATT
780












CCCCACAGTTCCACGCGTGGCGGATGT


RB1
NM_000321
3
13
+
58
+
0
37
1
0
CGCGAATGCCTTGACCTAGATGAGATGTCGTTC
781












ACTTTTACTGAGACGCGTGGCGGATGT


RB1
NM_000321
3
13
+
93
−
0
37
1
0
CGCGAATGCCAAGAAACTTTACCTGATTTCTAT
782












GTTTTTCTGTAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
8
1
+
−51
+
0
37
1
0
CGCGAATGCCCACCCACACGGCCACAGCTGAGG
783












GGCCCTGACCACACGCGTGGCGGATGT


RPS6KA1
NM_002953
8
1
+
−16
−
0
37
1
0
CGCGAATGCCCTCCTCATCCAGAAGGATGCTGT
784












AATAGAGAAATAACGCGTGGCGGATGT


RPS6KA1
NM_002953
8
1
+
19
+
0
37
1
0
CGCGAATGCCGGCCACATCAAACTCACTGGTGA
785












GTGGAGGGCGCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
8
1
+
54
−
0
37
1
0
CGCGAATGCCCCTTGTCCTGTCCTCCCCTGGGTC
786












CCGAGGGGGCAACGCGTGGCGGATGT


RBBP8
NM_002894
14
18
+
−8
+
7
37
1
0
CGCGAATGCCTTATTTAGGATGGCAGTCAGTCA
787












AAATTAGGAGGAACGCGTGGCGGATGT


RBBP8
NM_002894
14
18
+
23
−
0
37
1
0
CGCGAATGCCTAACCAATGTACAGTCCATGTCC
788












ACTGTCTCTCCTACGCGTGGCGGATGT


RBBP8
NM_002894
14
18
+
58
+
0
37
1
0
CGCGAATGCCGTGAAACCGTTCTCTTAAAAATG
789












AAGAAGCAAGAGACGCGTGGCGGATGT


RBBP8
NM_002894
14
18
+
93
−
0
37
1
0
CGCGAATGCCAAACAGATCTTACTTGAACTTTTT
790












TCTCCCTTCTGACGCGTGGCGGATGT


KSR2
NM_173598
4
12
−
−4
+
0
37
1
0
CGCGAATGCCCCAGGCGGGAGGACAAACTGCG
791












CATCCAGAATGGCACGCGTGGCGGATGT


KSR2
NM_173598
4
12
−
31
−
0
37
1
0
CGCGAATGCCTGGCGGATGATCTCTGGTGCCAG
792












GTGGCATAGCCAACGCGTGGCGGATGT


KSR2
NM_173598
4
12
−
66
+
0
37
1
0
CGCGAATGCCGCTGTCCCCCGACACAGAGGAGG
793












ATAAGCTCCCCTACGCGTGGCGGATGT


KSR2
NM_173598
4
12
−
101
−
0
37
1
0
CGCGAATGCCTTACCCAAGGGCAAAGACGTCAG
794












AGTGCTTGGAGAACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
0
+
0
37
1
0
CGCGAATGCCGTTTTGCTATCGGCATGCCAGTGT
795












GTGAATTTGATACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
35
−
0
37
1
0
CGCGAATGCCCTTCGGAAGTCCTGTACTTCTGG
796












ATCTTTAACCATACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
70
+
0
37
1
0
CGCGAATGCCAAATATTCTGAACGTTTGTAAAG
797












AAGCTGTGGATCACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
105
−
0
37
1
0
CGCGAATGCCCATTGCTCTACTATGAGGTGAAT
798












TGAGGTCCCTAAACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
140
+
0
37
1
0
CGCGAATGCCTATGTCTATCCTCCAAATGTAGA
799












ATCTTCACCAGAACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
175
−
0
37
1
0
CGCGAATGCCCTTTATCTAATTTATTATATATGT
800












GCTTTGGCAATACGCGTGGCGGATGT


PIK3CA
NM_006218
3
3
+
210
+
0
37
1
0
CGCGAATGCCGTAAGAAAATGACTAATCTACTC
801












TAATCATTACTAACGCGTGGCGGATGT


KSR2
NM_173598
6
12
−
−22
+
0
37
1
0
CGCGAATGCCATCTTGTCCTTTTGCTTTTCAGCC
802












TCTGTAAGGGAACGCGTGGCGGATGT


KSR2
NM_173598
6
12
−
13
−
0
37
1
0
CGCGAATGCCATTTTGGCATCCCTCACAACGGA
803












ATAGAGCGTCCGACGCGTGGCGGATGT


KSR2
NM_173598
6
12
−
48
+
0
37
1
0
CGCGAATGCCCGTTTTGGATGTCAACAAAACCA
804












GGCAGATTGCTCACGCGTGGCGGATGT


KSR2
NM_173598
6
12
−
83
−
0
37
1
0
CGCGAATGCCAGGCCCAAGAGCCGACAGTACCT
805












TCACAATTTCTTACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
0
+
0
37
1
0
CGCGAATGCCGTGAAGTCAGCACTGTGCTTAAG
806












CTGGATAACACAACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
35
−
0
37
1
0
CGCGAATGCCGGGCCACATGGCTTCCAAGACGT
807












CTGCCCCACCACACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
70
+
0
37
1
0
CGCGAATGCCCAATGCCTGGGACCAGAGCTTCA
808












CTCTGGAGCTGGACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
105
−
0
37
1
0
CGCGAATGCCTAGGCCCTGCTGTCCTCCAACGC
809












AGCTCACCCTTTACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
140
+
0
37
1
0
CGCGAATGCCGAGGAGGAGGGGTTCCATGCCTC
810












TGGCACCCTGAGACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
175
−
0
37
1
0
CGCGAATGCCTTCCCGTGCCTGGAGGGAGAGGA
811












AGAGGGCCATCAACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
210
+
0
37
1
0
CGCGAATGCCCTGGAGTTGGCTGTGTTCTGGCG
812












GGACCAGCGGGGACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
245
−
0
37
1
0
CGCGAATGCCAATCCTCCAACTTCAGGAATTTG
813












AGGGCACACAGGACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
280
+
0
37
1
0
CGCGAATGCCTCTTGGACAATGAGAGGCATGAG
814












GTGCAGCTGGACACGCGTGGCGGATGT


PKN1
NM_002741
9
19
+
315
−
0
37
1
0
CGCGAATGCCTATACCTCAGCCACCAGGCAGCC
815












CTGGGGTTCCATACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
0
+
0
37
1
0
CGCGAATGCCTGATGTCGTCATCGTGGAGCGTG
816












GGAAGGGCGACGACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
35
−
0
37
1
0
CGCGAATGCCCTTCATCCTGCCAAACTTCCTCCT
817












CTCGGGAACACACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
70
+
0
37
1
0
CGCGAATGCCCTCCTGCAGTTCTGTGAGAACCA
818












CCGGCCTGCCTAACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
105
−
0
37
1
0
CGCGAATGCCGGATGAGTGCCGTCTTCTTATTCC
819












AGGTACCCCAGACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
140
+
0
37
1
0
CGCGAATGCCGCGCGCGAGACCCCTGGGCCCAG
820












GACACGGTGAGCACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
175
−
0
37
1
0
CGCGAATGCCTGAGGAGGTACGGGGACGGAGG
821












CACTCTGGGGCTAACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
210
+
0
37
1
0
CGCGAATGCCCTGTGCCCCTTTCCTCCAGCCCCA
822












AAGACAGTTGTACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
245
−
0
37
1
0
CGCGAATGCCCCAGGAGCTTCTGAGAAAAGAAA
823












CACATGGGACTCACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
280
+
0
37
1
0
CGCGAATGCCACTATGAGGTGGACAGTGATGAG
824












GAGTGGGAAGAAACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
315
−
0
37
1
0
CGCGAATGCCCCCTCACTGTGGGACAGGGACTC
825












CCCAGGCTCCTCACGCGTGGCGGATGT


CHAF1A
NM_005483
10
19
+
350
+
0
37
1
0
CGCGAATGCCGGTAAGGATGTGCCCCAGCTGTC
826












TTCACTCACAGAACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
0
+
0
37
1
0
CGCGAATGCCAGTTATGAAAAAATTCACCTGGA
827












CTTCCTGAAGAGACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
35
−
0
37
1
0
CGCGAATGCCAGTCCACACGCATGCGTGCCACA
828












GCAGGATGGTCAACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
70
+
0
37
1
0
CGCGAATGCCCAGACAATGCATACATTGGTGTC
829












ACCTACAAAAACACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
105
−
0
37
1
0
CGCGAATGCCAGACCACCCTCCCAGTCCTTCAG
830












CTTGTCTTCCTCACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
140
+
0
37
1
0
CGCGAATGCCGGATGAGCAGAGACTGAGCGCTG
831












ACAGTGGCTACAACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
175
−
0
37
1
0
CGCGAATGCCCTCAGGGACAGGGTCAATGTCAG
832












GCAGAGGAATGAACGCGTGGCGGATGT


PDGFRA
NM_006206
22
4
+
210
+
0
37
1
0
CGCGAATGCCGAGGAGGACCTGGGCAAGAGGA
833












ACAGACACAGGTAACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
0
+
0
37
1
0
CGCGAATGCCCTTTTATTCCGCCTGCGTGGTGCT
834












GGGCCTACAGTACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
35
−
0
37
1
0
CGCGAATGCCCACACACCTGTAGACGATCTTGT
835












GTTCGTGAAGAAACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
70
+
0
37
1
0
CGCGAATGCCCGTGTGTGCATGCATGTGCACAC
836












TGCCCGTTGTGGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
105
−
0
37
1
0
CGCGAATGCCGGAAAGAGGGATGAGCGGGGGT
837












CTGGGTCCTGTCCACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
140
+
0
37
1
0
CGCGAATGCCACCCCTCCAACCCCCAACAGGGA
838












CCTGAAGTTGGAACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
175
−
0
37
1
0
CGCGAATGCCTCTTGACGTAGCCCTCGGTGTCC
839












AGGAGCAAATTGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
210
+
0
37
1
0
CGCGAATGCCTCGCAGACTTTGGCCTCTGCAAG
840












GAGGGTGAGGGGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
245
−
0
37
1
0
CGCGAATGCCTTCCTTCCCCCTCCTTTGTCTAAT
841












CCAGAGGCCAGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
280
+
0
37
1
0
CGCGAATGCCTGGGGTCCCTAACCCTATTCGGG
842












GTGCCCCCCACCACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
315
−
0
37
1
0
CGCGAATGCCGGTTGTCCACACAGCCAGCCCTG
843












TCCCAGGGTCAGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
350
+
0
37
1
0
CGCGAATGCCCCACATTGGGCTGAGTGACTCCT
844












CTGGCCCCCATAACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
385
−
0
37
1
0
CGCGAATGCCAGCCCATCCCTGGGGACAGCAAG
845












GCCATGTGAGGTACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
420
+
0
37
1
0
CGCGAATGCCATGGGGACCGGACCAGCACATTC
846












TGTGGGACCCCGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
455
−
0
37
1
0
CGCGAATGCCGACGTGTCCGTCAGCACCTCAGG
847












GGCCAGGAACTCACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
490
+
0
37
1
0
CGCGAATGCCGTACACGCGAGCTGTGGACTGGT
848












GGGGACTGGGTGACGCGTGGCGGATGT


PKN1
NM_002741
19
19
+
525
−
0
37
1
0
CGCGAATGCCTCTCACCTCGCCAACCAGCATCT
849












CGTAGAGCAGCAACGCGTGGCGGATGT


EPHA7
NM_004440
10
6
−
−16
+
0
37
1
0
CGCGAATGCCTTTCCTCTGTTTACAGCTACAGCT
850












GTCTCCAGTGAACGCGTGGCGGATGT


EPHA7
NM_004440
10
6
−
19
−
0
37
1
0
CGCGAATGCCCAGCAACCACAGCAATGATAATA
851












ACAGGATTCTGTACGCGTGGCGGATGT


EPHA7
NM_004440
10
6
−
54
+
0
37
1
0
CGCGAATGCCTAGCTGGGACCATCATTTTGGTG
852












TTCATGGTCTTTACGCGTGGCGGATGT


EPHA7
NM_004440
10
6
−
89
−
0
37
1
0
CGCGAATGCCAACTTTGTGTTCTACCTTCTCCCA
853












ATGATGAAGCCACGCGTGGCGGATGT


PIK3CA
NM_006218
11
3
+
−29
+
0
37
1
0
CGCGAATGCCTTTATGTTTATTTTGTTTCTCCCAC
854












ACAGACACTAACGCGTGGCGGATGT


PIK3CA
NM_006218
11
3
+
6
−
0
37
1
0
CGCGAATGCCGCAATTTGGGTAGAATTTCGGGG
855












ATAGTTACACAAACGCGTGGCGGATGT


PIK3CA
NM_006218
11
3
+
39
+
0
37
1
0
CGCGAATGCCGCTTCTGTCTGTTAAATGGAATTC
856












TAGAGATGAAGACGCGTGGCGGATGT


PIK3CA
NM_006218
11
3
+
76
−
0
0
2
0
CGCGAATGCCTTATCTAGTAATCTCAAACATAC
857












ATTTACCTGGGCACGCGTGGCGGATGT


NTRK3
NM_001012338
10
15
−
−58
+
0
37
1
0
CGCGAATGCCTGTGTCTGTTCTGGTTTTTATTAA
858












ATTTGTTAATTACGCGTGGCGGATGT


NTRK3
NM_001012338
10
15
−
−23
−
0
37
1
0
CGCGAATGCCTATCCGTGCTCTCTGCAAAAAAA
859












GGACAAAGAGATACGCGTGGCGGATGT


NTRK3
NM_001012338
10
15
−
12
+
0
37
1
0
CGCGAATGCCACTTTATCTTGTGTAAGTCTGCTT
860












TACCTGTTGCTACGCGTGGCGGATGT


NTRK3
NM_001012338
10
15
−
47
−
0
37
1
0
CGCGAATGCCTAGTTACCGATAGTATCAGAATA
861












AATCAGTTTCAAACGCGTGGCGGATGT


RBBP8
NM_002894
9
18
+
−21
+
0
37
1
0
CGCGAATGCCTGGTTTATTATTTATTCTTAGAAG
862












CACATGGAACAACGCGTGGCGGATGT


RBBP8
NM_002894
9
18
+
14
−
0
37
1
0
CGCGAATGCCAAATTAAAAGATGACTTATCAGG
863












GGTATAGCTGCTACGCGTGGCGGATGT


RBBP8
NM_002894
9
18
+
49
+
0
37
1
0
CGCGAATGCCAGCTACAGTTGTTGCTGAAACAC
864












TTGGACTTGGTGACGCGTGGCGGATGT


RBBP8
NM_002894
9
18
+
84
−
0
37
1
0
CGCGAATGCCCCAAACTAAACAATTACTTACAG
865












ATTCTTCTTGAAACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
0
+
0
37
1
0
CGCGAATGCCACCCTGGCCGGCTCCTGTGGGAA
866












GTTTGCCCCCTTACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
35
−
0
37
1
0
CGCGAATGCCGCCGAGGGGCCAGGACCATGTGC
867












TCTTTAATTTCAACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
70
+
0
37
1
0
CGCGAATGCCGTCGGACCGCTTTCCATCCAGAC
868












CTCTGCAGTCAGACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
105
−
0
37
1
0
CGCGAATGCCAACTCGCCGCTCTGCTGCTGGAG
869












GAGCTGGTCCAGACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
140
+
0
37
1
0
CGCGAATGCCCTCCTTCTTGAAAGACCTCAAAG
870












GCCGGCAGCCCCACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
175
−
0
37
1
0
CGCGAATGCCATTCCGGGTGGAAACGTGCGTGG
871












GTCCGGACCTCAACGCGTGGCGGATGT


CHAF1A
NM_005483
8
19
+
210
+
0
37
1
0
CGCGAATGCCGCAGATATTTTTAACAGGTCAGA
872












GCCTGAGGAGGTACGCGTGGCGGATGT


RPS6KA1
NM_002953
17
1
+
0
+
0
37
1
0
CGCGAATGCCGTGTATGATGATGGCAAACACGT
873












GTACCTGGTGACACGCGTGGCGGATGT


RPS6KA1
NM_002953
17
1
+
35
−
0
37
1
0
CGCGAATGCCTCTTGTCCAGCAGCTCCCCACCCC
874












GCATCAGCTCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
17
1
+
70
+
0
37
1
0
CGCGAATGCCTCCTGCGGCAGAAGTTCTTCTCA
875












GAGCGGGAGGCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
17
1
+
105
−
0
37
1
0
CGCGAATGCCTCCACAGTTTTGCCAATGGTGTG
876












CAGGACAAAGCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
17
1
+
140
+
0
37
1
0
CGCGAATGCCGTATCTGCACTCACAGGGGGTGA
877












GTCTGGATTCGGACGCGTGGCGGATGT


EPHA7
NM_004440
6
6
−
−39
+
0
37
1
0
CGCGAATGCCCAGGTTACGACTCCTGAAACTTT
878












TTTTTTTTAATTACGCGTGGCGGATGT


EPHA7
NM_004440
6
6
−
−4
−
0
37
1
0
CGCGAATGCCATGAACTCTATTACTATCATGACT
879












GGTTTCCCTAAACGCGTGGCGGATGT


EPHA7
NM_004440
6
6
−
31
+
0
37
1
0
CGCGAATGCCGGAAAATGGAGCCCTAGATGCAT
880












TTCTCAGGGTAAACGCGTGGCGGATGT


EPHA7
NM_004440
6
6
−
66
−
0
37
1
0
CGCGAATGCCATAGTATATTTAGAATAAGTGGA
881












TCACTTTGGTACACGCGTGGCGGATGT


RB1
NM_000321
21
13
+
−18
+
0
37
1
0
CGCGAATGCCCATCAATTTATTTACTAGATTATG
882












ATGTGTTCCATACGCGTGGCGGATGT


RB1
NM_000321
21
13
+
17
−
0
37
1
0
CGCGAATGCCTAAGGTCTATATTCTTCACTTTGC
883












ATATGCCATACACGCGTGGCGGATGT


RB1
NM_000321
21
13
+
52
+
0
37
1
0
CGCGAATGCCAATTCAAAATCATTGTAACAGCA
884












TACAAGGATCTTACGCGTGGCGGATGT


RB1
NM_000321
21
13
+
87
−
0
37
1
0
CGCGAATGCCTATGGAAAATTACCTACCTCCTG
885












AACAGCATGAGGACGCGTGGCGGATGT


KSR2
NM_173598
11
12
−
0
+
0
37
1
0
CGCGAATGCCGACCACATCCCTGTCCCTTACCA
886












GCCAGACTCCAGACGCGTGGCGGATGT


KSR2
NM_173598
11
12
−
35
−
0
37
1
0
CGCGAATGCCAGGGCGTGGAGGACGTCGTGGA
887












GGAGGGGTTGCTGACGCGTGGCGGATGT


KSR2
NM_173598
11
12
−
70
+
0
37
1
0
CGCGAATGCCCCTCGCCAGCACCCCCCCTCCCTC
888












CTAGTGCCACGACGCGTGGCGGATGT


KSR2
NM_173598
11
12
−
105
−
0
37
1
0
CGCGAATGCCGTGCACTGTGGGGAAGGGTGTAG
889












GGGAGAAGGCGGACGCGTGGCGGATGT


KSR2
NM_173598
11
12
−
140
+
0
37
1
0
CGCGAATGCCACGGCAGCAGAAGAACTTCAACC
890












TGCCAGGTACCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
12
1
+
−38
+
0
37
1
0
CGCGAATGCCGCCAGCCAGGGACAGACCCTTCA
891












TTTGGGCTCTTTACGCGTGGCGGATGT


RPS6KA1
NM_002953
12
1
+
−3
−
0
37
1
0
CGCGAATGCCCTTGATTTCCTCTGCCCCATCAGG
892












GCCGGAGCCTGACGCGTGGCGGATGT


RPS6KA1
NM_002953
12
1
+
32
+
0
37
1
0
CGCGAATGCCCGGCATGTCTTCTACTCCACCATT
893












GACTGGAATGTACGCGTGGCGGATGT


RPS6KA1
NM_002953
12
1
+
67
−
0
37
1
0
CGCGAATGCCCCCTGGCCAGAGCCCTGGTGTGG
894












GTGGACACACTCACGCGTGGCGGATGT


PALB2
NM_024675
2
16
−
0
+
0
37
1
0
CGCGAATGCCGGGCTTCTCTTTATTGTCCTGAGT
895












CATCCCTGTGCACGCGTGGCGGATGT


PALB2
NM_024675
2
16
−
35
−
0
37
1
0
CGCGAATGCCGAAACACAGGGCTTCGCAACGAC
896












TCACTCTCTTTGACGCGTGGCGGATGT


PALB2
NM_024675
2
16
−
70
+
0
37
1
0
CGCGAATGCCAGCTCATTGTGATTAACCCTAAG
897












ACGACTCTCAGCACGCGTGGCGGATGT


PALB2
NM_024675
2
16
−
105
−
0
37
1
0
CGCGAATGCCTGCCCTGGAGGAAGACAGTACAG
898












CATCACACCCACACGCGTGGCGGATGT


PALB2
NM_024675
2
16
−
140
+
0
37
1
0
CGCGAATGCCGGCTGGCAGGCAAGTGTGCATAA
899












CTGCTACTCTATACGCGTGGCGGATGT


EPHA3
NM_005233
16
3
+
0
+
0
37
1
0
CGCGAATGCCGCCATCAAACCTTCTTCTGGACC
900












AAAGCAATGTGGACGCGTGGCGGATGT


EPHA3
NM_005233
16
3
+
35
−
0
37
1
0
CGCGAATGCCAAGCCAGTCACCTGTTGTGCGGA
901












AGGTAGTGATATACGCGTGGCGGATGT


EPHA3
NM_005233
16
3
+
70
+
0
37
1
0
CGCGAATGCCAATGGTGTCTGGACAGCACACTG
902












CAAGGAAATCTTACGCGTGGCGGATGT


EPHA3
NM_005233
16
3
+
105
−
0
37
1
0
CGCGAATGCCCTATTGTGTCACAAGAACTGTAC
903












TCCACACCCGTGACGCGTGGCGGATGT


EPHA3
NM_005233
16
3
+
140
+
0
37
1
0
CGCGAATGCCCCAAGATTTCCACAGAGTAAGAA
904












AAAAAAATTCATACGCGTGGCGGATGT


RET
NM_020630
12
10
+
0
+
0
37
1
0
CGCGAATGCCGAGGATCCAAAGTGGGAATTCCC
905












TCGGAAGAACTTACGCGTGGCGGATGT


RET
NM_020630
12
10
+
35
−
0
37
1
0
CGCGAATGCCCAAATTCGCCTTCTCCTAGAGTTT
906












TTCCAAGAACCACGCGTGGCGGATGT


RET
NM_020630
12
10
+
70
+
0
37
1
0
CGCGAATGCCGAAAAGTGGTCAAGGCAACGGC
907












CTTCCATCTGAAAACGCGTGGCGGATGT


RET
NM_020630
12
10
+
105
−
0
37
1
0
CGCGAATGCCATCTTCACGGCCACCGTGGTGTA
908












CCCTGCTCTGCCACGCGTGGCGGATGT


RET
NM_020630
12
10
+
140
+
0
37
1
0
CGCGAATGCCGCTGAAAGGTACCTGCCAGGCAC
909












AGGCACAGTGCCACGCGTGGCGGATGT


RB1
NM_000321
27
13
+
−33
+
0
37
1
0
CGCGAATGCCAATGCTGTTAACAGTTCTTCATCC
910












TTTTTCCAGCTACGCGTGGCGGATGT


RB1
NM_000321
27
13
+
2
−
0
37
1
0
CGCGAATGCCTTCATTTTCTGCTTTTGCATTCGT
911












GTTCGAGTAGAACGCGTGGCGGATGT


RB1
NM_000321
27
13
+
37
+
0
37
1
0
CGCGAATGCCTGATAGCATGGATACCTCAAACA
912












AGGAAGAGAAATACGCGTGGCGGATGT


RB1
NM_000321
27
13
+
72
−
0
37
1
0
CGCGAATGCCGTGTACACAGTGTCCACCAAGGT
913












CCTGAGATCCTCACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
0
+
0
37
1
0
CGCGAATGCCAGCTCCCCCATCCAGGAATCCAC
914












TGCTCCCGAGCTACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
35
−
0
37
1
0
CGCGAATGCCGGGCGGGGCCTGGGGTCTCCTGG
915












GTCTCCGAAGGCACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
70
+
0
37
1
0
CGCGAATGCCTGTGCAGGTGACACCACTCCCTG
916












GCCCCCTGTCCAACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
105
−
0
37
1
0
CGCGAATGCCCCACTGGTGAGAACAAGGACAGC
917












GGGCAGGGGTGGACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
140
+
0
37
1
0
CGCGAATGCCCCTCTCTCCACAGCCCTCTGAGG
918












AAGTCACCTCTGACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
175
−
0
37
1
0
CGCGAATGCCCCCAGCACCGCCAGGAACTTGAA
919












ATCTTCGAGGGTACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
210
+
0
37
1
0
CGCGAATGCCCCGGGGTCATTTTGGGAAGGTGA
920












GGTGGAGGGCAGACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
245
−
0
37
1
0
CGCGAATGCCCGGCCAGCCAGGGACCTGGGGG
921












GTCTCCCAATTCCACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
280
+
0
37
1
0
CGCGAATGCCCTAAAACCACCCCTGCCCACTGT
922












GGTTCCAGGTGCACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
315
−
0
37
1
0
CGCGAATGCCGAACAGCTCCCCACTGGGCCGGA
923












ATTCGGAGAGGAACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
350
+
0
37
1
0
CGCGAATGCCGCCATCAAGGCTCTGAAGAAAGG
924












GGACATTGTGGCACGCGTGGCGGATGT


PKN1
NM_002741
15
19
+
385
−
0
37
1
0
CGCGAATGCCCCACGGCAGGTCCCCACCTCTCC
925












ACCTCGTCTCGGACGCGTGGCGGATGT


CHAF1A
NM_005483
1
19
+
−44
+
0
37
1
0
CGCGAATGCCCGCGCCTCCGCCGCCTGAGAGGA
926












GGTCGAGCTGCCACGCGTGGCGGATGT


CHAF1A
NM_005483
1
19
+
−9
−
0
37
1
0
CGCGAATGCCGCCCCGCACTCCAGCTCCTCCAG
927












CATCGCCCCGGCACGCGTGGCGGATGT


CHAF1A
NM_005483
1
19
+
26
+
0
37
1
0
CGCGAATGCCGCCCGGCGCCAGGGGAGCCGCCA
928












CAGGTCGGTTCGACGCGTGGCGGATGT


CHAF1A
NM_005483
1
19
+
57
−
13
37
1
0
CGCGAATGCCGCCGCGCCCCCCCTTCCCCTCGG
929












CGCGGGCCCGAAACGCGTGGCGGATGT


GUCY2F
NM_001522
11
X
−
0
+
0
37
1
0
CGCGAATGCCGGCATGAAGTACTTACACCACAG
930












AGAGTTTGTTCAACGCGTGGCGGATGT


GUCY2F
NM_001522
11
X
−
35
−
0
37
1
0
CGCGAATGCCCATCTACCACACAGTTTCGAGAC
931












TTTAGCCTCCCAACGCGTGGCGGATGT


GUCY2F
NM_001522
11
X
−
70
+
0
37
1
0
CGCGAATGCCGGCGTTTTGTACTAAAAGTGACA
932












GATTATGGCTTTACGCGTGGCGGATGT


GUCY2F
NM_001522
11
X
−
105
−
0
37
1
0
CGCGAATGCCTCTTCAGAGAGTCTCAGCATTTCT
933












AAGATGTCGTTACGCGTGGCGGATGT


GUCY2F
NM_001522
11
X
−
140
+
0
37
1
0
CGCGAATGCCGGAATCTTCTATGGAAGGTAAGC
934












AATGAATTGTACACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
0
+
0
37
1
0
CGCGAATGCCGATTACATCCACAGTACCCACGG
935












CAAGGAGATGGAACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
35
−
0
37
1
0
CGCGAATGCCGCTTGCCCGGGACTTTGACTGTT
936












GTTCGCAGCAAGACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
70
+
0
37
1
0
CGCGAATGCCGGCCCCCGAGGGCCATCTCTGCC
937












TTTGGCCCCTCAACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
105
−
0
37
1
0
CGCGAATGCCGTGCTCATGTCCTTGACGAGCCC
938












GTTAATGCTGGCACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
140
+
0
37
1
0
CGCGAATGCCTGTCCAGATGGGTGAAGGCCTGG
939












GTGAGTAAGGTTACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
175
−
0
37
1
0
CGCGAATGCCCCCAGCCCTGGCCCCTCTTCTGCT
940












CCTATGTCATAACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
210
+
0
37
1
0
CGCGAATGCCGAAAAAGAGGGGTAAGAGGGAA
941












GAGGGGGCTGGCAACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
245
−
0
37
1
0
CGCGAATGCCGTCCCTCTTTATCACCCAGCTTCC
942












TACTCGCCCAGACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
280
+
0
37
1
0
CGCGAATGCCCACATAAGGAATGGGGCCAGAA
943












GAAAGAGGCTCACACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
315
−
0
37
1
0
CGCGAATGCCAGTAGTGGCTTCTGTCGGAAGAA
944












GATGAAACCTCAACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
350
+
0
37
1
0
CGCGAATGCCCCCATGCCAAGCCCTAGCCCCAG
945












CCCCAGTTCCCTACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
385
−
0
37
1
0
CGCGAATGCCGCAGGTGTTTGGATGTCTGATCT
946












GGTGGTGGCTGCACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
420
+
0
37
1
0
CGCGAATGCCTGAAGCCAGACCGGAATTTGGCC
947












CGAGCCCTCAGCACGCGTGGCGGATGT


CENTG1
NM_014770
8
12
−
455
−
0
37
1
0
CGCGAATGCCAGACAGCAGATGGAAGGTCATCC
948












CCACTGACCCGTACGCGTGGCGGATGT


EPHA7
NM_004440
8
6
−
−7
+
0
37
1
0
CGCGAATGCCATTACAGTTAAATTTCCAGGCAC
949












CAAAACCTACATACGCGTGGCGGATGT


EPHA7
NM_004440
8
6
−
28
−
0
37
1
0
CGCGAATGCCCAGCTCTATTTGGGTCCTCATAG
950












GTTTCAGGGTCAACGCGTGGCGGATGT


EPHA7
NM_004440
8
6
−
63
+
0
37
1
0
CGCGAATGCCTCCATCAATTCGCCAAGGAGCTA
951












GATGCCTCCTGTACGCGTGGCGGATGT


EPHA7
NM_004440
8
6
−
98
−
0
37
1
0
CGCGAATGCCGCCTTACCTGCACCAATCACACG
952












CTCAATTTTAATACGCGTGGCGGATGT


EPHB1
NM_004441
10
3
+
−8
+
0
37
1
0
CGCGAATGCCCCAACAAGGCTCCCCAGGGATGA
953












AGATCTACATTGACGCGTGGCGGATGT


EPHB1
NM_004441
10
3
+
27
−
0
37
1
0
CGCGAATGCCGACAGCTTCGTTGGGATCCTCGT
954












AAGTGAAGGGGTACGCGTGGCGGATGT


EPHB1
NM_004441
10
3
+
62
+
0
37
1
0
CGCGAATGCCCGGGAGTTTGCCAAGGAGATTGA
955












TGTATCTTTTGTACGCGTGGCGGATGT


EPHB1
NM_004441
10
3
+
97
−
0
37
1
0
CGCGAATGCCGAGCCATACCTGCTCCGATGACC
956












TCTTCAATTTTCACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
0
+
0
37
1
0
CGCGAATGCCGAACACTGTCCATTGGCATGGGG
957












AAATATAAACTTACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
35
−
0
37
1
0
CGCGAATGCCTTTTTCCAGATACTAGAGTGTCTG
958












TGTAATCAAACACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
70
+
0
37
1
0
CGCGAATGCCTGGCTTTGAATCTTTGGCCAGTAC
959












CTCATGGATTAACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
105
−
0
37
1
0
CGCGAATGCCGATCCAGTAACACCAATAGGGTT
960












CAGCAAATCTTCACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
140
+
0
37
1
0
CGCGAATGCCAAATCCAAATAAAGTAAGGTTTT
961












TATTGTCATAAAACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
175
−
0
37
1
0
CGCGAATGCCAGAGAGAAGGTTTGACTGCCATA
962












AAAAATATCTAAACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
210
+
0
37
1
0
CGCGAATGCCTATGTATATATAATAGCTTTTCTT
963












CCATCTCTTAGACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
245
−
0
37
1
0
CGCGAATGCCAACCAGTCAAACTCCAACTCTAA
964












GCATGGAGTTTCACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
280
+
0
37
1
0
CGCGAATGCCCAGCAGTGTGGTAAAGTTCCCAG
965












ATATGTCAGTGAACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
315
−
0
37
1
0
CGCGAATGCCTTCTCGGGATACAGACCAATTGG
966












CATGCTCTTCAAACGCGTGGCGGATGT


PIK3CA
NM_006218
9
3
+
350
+
0
37
1
0
CGCGAATGCCGCAGGATTTAGCTATTCCCACGC
967












AGGACTGGTAAGACGCGTGGCGGATGT


RET
NM_020630
16
10
+
−34
+
0
37
1
0
CGCGAATGCCTAACTTCAATGTCTTTATTCCATC
968












TTCTCTTTAGGACGCGTGGCGGATGT


RET
NM_020630
16
10
+
1
−
0
37
1
0
CGCGAATGCCGGATTCAATTGCCATCCATTTAA
969












CTGGAATCCGACACGCGTGGCGGATGT


RET
NM_020630
16
10
+
36
+
0
37
1
0
CGCGAATGCCCTTTTTGATCATATCTACACCACG
970












CAAAGTGATGTACGCGTGGCGGATGT


RET
NM_020630
16
10
+
71
−
0
37
1
0
CGCGAATGCCAACCTCCACCCCAAGAGAGCAAC
971












ACCCACACTTACACGCGTGGCGGATGT


PIK3CA
NM_006218
17
3
+
−30
+
0
37
1
0
CGCGAATGCCAAATGGTGATACATATTATTTGA
972












ATTTCAGATTTAACGCGTGGCGGATGT


PIK3CA
NM_006218
17
3
+
5
−
0
37
1
0
CGCGAATGCCATACGAATAATTTGAAGTGTTAG
973












CATATCTTGCCGACGCGTGGCGGATGT


PIK3CA
NM_006218
17
3
+
40
+
0
37
1
0
CGCGAATGCCTATGGAAAATATCTGGCAAAATC
974












AAGGTCTTGATCACGCGTGGCGGATGT


PIK3CA
NM_006218
17
3
+
75
−
0
37
1
0
CGCGAATGCCCAACATACAGGTTGCCTTACTGG
975












TTACCTACCGAAACGCGTGGCGGATGT


RB1
NM_000321
4
13
+
−10
+
0
37
1
0
CGCGAATGCCTCCTTTGTAGTGTCCATAAATTCT
976












TTAACTTACTAACGCGTGGCGGATGT


RB1
NM_000321
4
13
+
25
−
0
37
1
0
CGCGAATGCCGCATTATCAACTTTGGTACTGGT
977












ATCAATTTCTTTACGCGTGGCGGATGT


RB1
NM_000321
4
13
+
60
+
0
37
1
0
CGCGAATGCCTATGTCAAGACTGTTGAAGAAGT
978












ATGATGTATTGTACGCGTGGCGGATGT


RB1
NM_000321
4
13
+
95
−
0
37
1
0
CGCGAATGCCTTTACTTTACCTTTCCAATTTGCT
979












GAAGAGTGCAAACGCGTGGCGGATGT


RPS6KA1
NM_002953
21
1
+
−1
+
0
37
1
0
CGCGAATGCCGGACCTGGTGTCCAAGATGCTAC
980












ACGTGGATCCCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
21
1
+
34
−
0
37
1
0
CGCGAATGCCATGCTGCAGAACCTGCTTAGCTG
981












TGAGGCGCTGGTACGCGTGGCGGATGT


RPS6KA1
NM_002953
21
1
+
69
+
0
37
1
0
CGCGAATGCCCCATGGGTCACCCAGAAAGACAA
982












GCTTCCCCAAAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
21
1
+
104
−
0
37
1
0
CGCGAATGCCCCTTCACAAGCTGTAGGTCCTGG
983












TGGGACAGCTGGACGCGTGGCGGATGT


NFKB1
NM_003998
3
4
+
−30
+
0
37
1
0
CGCGAATGCCATTGAAACATTTAAATGTTCTTCT
984












TTACAGATGTTACGCGTGGCGGATGT


NFKB1
NM_003998
3
4
+
5
−
0
37
1
0
CGCGAATGCCTAAATATTGTATGAGTCAAAGAA
985












GGATCCAAATGAACGCGTGGCGGATGT


NFKB1
NM_003998
3
4
+
40
+
0
37
1
0
CGCGAATGCCATCCAGAAGTATTTCAACCACAG
986












ATGGCACTGCCAACGCGTGGCGGATGT


NFKB1
NM_003998
3
4
+
75
−
0
37
1
0
CGCGAATGCCACAACAGGGTAACAGGGATGAG
987












TTTTCTTACCTGTACGCGTGGCGGATGT


EPHA3
NM_005233
17
3
+
−17
+
0
37
1
0
CGCGAATGCCGTTTTCTTTTTTTACAGTGACATG
988












AAAAAGGTTGGACGCGTGGCGGATGT


EPHA3
NM_005233
17
3
+
18
−
0
37
1
0
CGCGAATGCCTGATGATCTTCTTCTGTGGCCCAA
989












CCACGGTGACAACGCGTGGCGGATGT


EPHA3
NM_005233
17
3
+
53
+
0
37
1
0
CGCGAATGCCGTAGCATTAAAGCTCTAGAAACG
990












CAATCAAAGAATACGCGTGGCGGATGT


EPHA3
NM_005233
17
3
+
88
−
0
37
1
0
CGCGAATGCCCACTTCCGTCCCGTGCTTTACACG
991












GGAACTGGGCCACGCGTGGCGGATGT


NTRK3
NM_001007156
2
15
−
−6
+
19
37
1
0
CGCGAATGCCTGCCAGGGGTCTTTTCAAACATA
992












GACAATCATGGGACGCGTGGCGGATGT


NTRK3
NM_001007156
2
15
−
33
−
35
37
1
0
CGCGAATGCCTGGGACTAGATGATCTCTATTGT
993












CCTTCAAGTTTAACGCGTGGCGGATGT


NTRK3
NM_001007156
2
15
−
68
+
35
37
1
0
CGCGAATGCCTCAACTCACTATATATATGAGGA
994












ACCTGAGGTCCAACGCGTGGCGGATGT


NTRK3
NM_001007156
2
15
−
103
−
35
37
1
0
CGCGAATGCCCACCATGTGACCTTGGGTAAGAC
995












ACTTCCCCACTCACGCGTGGCGGATGT


EPHA4
NM_004438
3
2
−
0
+
0
37
1
0
CGCGAATGCCACCTAACACTGCCTTGTTGGATC
996












CAAGCTCCCCTGACGCGTGGCGGATGT


EPHA4
NM_004438
3
2
−
35
−
0
37
1
0
CGCGAATGCCGAGCCAATCGCCCACTGATACCA
997












CAGCAGAGAATTACGCGTGGCGGATGT


EPHA4
NM_004438
3
2
−
70
+
0
37
1
0
CGCGAATGCCCAGGCCATTAAAATGGACCGGTA
998












TAAGGATAACTTACGCGTGGCGGATGT


EPHA4
NM_004438
3
2
−
105
−
0
37
1
0
CGCGAATGCCCCACAGCCTCTAGTGTGGTATAA
999












CCAGCAGCTGTGACGCGTGGCGGATGT


EPHA4
NM_004438
3
2
−
140
+
0
37
1
0
CGCGAATGCCTGCACGTGAACCAGGAGTAAGTA
1000












CTCAACGATGTAACGCGTGGCGGATGT


EPHA3
NM_005233
9
3
+
−38
+
0
37
1
0
CGCGAATGCCTTCCTCTTATGTGTTCGCTTTCCTT
1001












GATTTACCTCACGCGTGGCGGATGT


EPHA3
NM_005233
9
3
+
−3
−
0
37
1
0
CGCGAATGCCCTGCCCCATGTTTTGACTTATAGC
1002












CACAGAACCTGACGCGTGGCGGATGT


EPHA3
NM_005233
9
3
+
32
+
0
37
1
0
CGCGAATGCCATGAAAAAAGACTTCATTTTGGC
1003












AATGGGCATTGTACGCGTGGCGGATGT


EPHA3
NM_005233
9
3
+
67
−
0
37
1
0
CGCGAATGCCGGTGAAGCAAAACAAAAAGCCA
1004












AGTTTAGAAACTTACGCGTGGCGGATGT


EPHB1
NM_004441
7
3
+
0
+
0
37
1
0
CGCGAATGCCGAACACAATGAGTTCAACTCCTC
1005












CATGGCCAGGAGACGCGTGGCGGATGT


EPHB1
NM_004441
7
3
+
35
−
0
37
1
0
CGCGAATGCCGCCGCAGCCCATCAATCCTTGCT
1006












GTGTTGGTCTGAACGCGTGGCGGATGT


EPHB1
NM_004441
7
3
+
70
+
0
37
1
0
CGCGAATGCCCTGGCATGGTATATGTGGTACAG
1007












GTGCGTGCCCGCACGCGTGGCGGATGT


EPHB1
NM_004441
7
3
+
105
−
0
37
1
0
CGCGAATGCCATCTTGCCACTGAACTTGCCGTA
1008












GCCAGCAACAGTACGCGTGGCGGATGT


EPHB1
NM_004441
7
3
+
140
+
0
37
1
0
CGCGAATGCCGTGCTTCCAGACTCTGACTGACG
1009












GTAAGGGTCGGGACGCGTGGCGGATGT


RB1
NM_000321
19
13
+
0
+
0
37
1
0
CGCGAATGCCGTATCTTTCTCCTGTAAGATCTCC
1010












AAAGAAAAAAGACGCGTGGCGGATGT


RB1
NM_000321
19
13
+
35
−
0
37
1
0
CGCGAATGCCTGCATTTGCAGTAGAATTTACAC
1011












GCGTAGTTGAACACGCGTGGCGGATGT


RB1
NM_000321
19
13
+
70
+
0
37
1
0
CGCGAATGCCGAGACACAAGCAACCTCAGCCTT
1012












CCAGACCCAGAAACGCGTGGCGGATGT


RB1
NM_000321
19
13
+
105
−
0
37
1
0
CGCGAATGCCTATAAAACAGTGAAAGAGAGGT
1013












AGATTTCAATGGCACGCGTGGCGGATGT


RB1
NM_000321
19
13
+
136
+
7
37
1
0
CGCGAATGCCTATAAAAAAGGTTAGTAGATGAT
1014












TATTTTCAAGAGACGCGTGGCGGATGT


RBBP8
NM_002894
18
18
+
0
+
0
37
1
0
CGCGAATGCCTATTATGCAGATATGCCAGCAGA
1015












AGAAAGAGAAAAACGCGTGGCGGATGT


RBBP8
NM_002894
18
18
+
35
−
0
37
1
0
CGCGAATGCCAGCGGAATCGGTGTCTTGAGCAG
1016












GAAGCCAATTTCACGCGTGGCGGATGT


RBBP8
NM_002894
18
18
+
70
+
0
37
1
0
CGCGAATGCCACATTCCACCCAACACACCAGAG
1017












AATTTTTGGGAAACGCGTGGCGGATGT


RBBP8
NM_002894
18
18
+
105
−
0
37
1
0
CGCGAATGCCCTTTCCATACAAGTCTGAGTGGA
1018












AGGAAAACCAACACGCGTGGCGGATGT


RBBP8
NM_002894
18
18
+
136
+
9
37
1
0
CGCGAATGCCAAAGAGGTGAGAGTATAGATTGT
1019












AACATTTTATAAACGCGTGGCGGATGT


KSR2
NM_173598
8
12
−
−46
+
0
37
1
0
CGCGAATGCCCCTGCCTTTTCACCTAGGGATCAC
1020












GTTTATTTTTCACGCGTGGCGGATGT


KSR2
NM_173598
8
12
−
−11
−
0
37
1
0
CGCGAATGCCTGAAGTAATGGATTTCCTTCCAA
1021












GCTGTAAAGAAAACGCGTGGCGGATGT


KSR2
NM_173598
8
12
−
24
+
0
37
1
0
CGCGAATGCCAATTGAAGTGGAGCCAACGTCGG
1022












AGGTGAGAATCAACGCGTGGCGGATGT


KSR2
NM_173598
8
12
−
59
−
0
37
1
0
CGCGAATGCCGGGCAGTCCCTGGAAAATGTCGC
1023












TTCACTGCTCTCACGCGTGGCGGATGT


RBBP8
NM_002894
10
18
+
−10
+
0
37
1
0
CGCGAATGCCACTCTTGAAGGAAACTCAAGGTC
1024












CCATGAGCCCCCACGCGTGGCGGATGT


RBBP8
NM_002894
10
18
+
21
−
0
37
1
0
CGCGAATGCCCCTTCCAGACAGTGGTAGAGCTC
1025












ATCACCAAGGGGACGCGTGGCGGATGT


RBBP8
NM_002894
10
18
+
56
+
0
37
1
0
CGCGAATGCCAAATCACAAGAAACAGCCTTTTG
1026












AGGAATCTACAAACGCGTGGCGGATGT


RBBP8
NM_002894
10
18
+
91
−
0
37
1
0
CGCGAATGCCTGCCCTTAATTACCTTAAACTATC
1027












TTCAGTATTTCACGCGTGGCGGATGT


RB1
NM_000321
9
13
+
−31
+
0
37
1
0
CGCGAATGCCAATGATCATGTTGTAACTTCATCT
1028












TTTTCAGGTGAACGCGTGGCGGATGT


RB1
NM_000321
9
13
+
4
−
0
37
1
0
CGCGAATGCCCATAAAAGGTATAAAATTTTTGA
1029












AATAAACATTTTACGCGTGGCGGATGT


RB1
NM_000321
9
13
+
39
+
0
37
1
0
CGCGAATGCCAATTCTCTTGGACTTGTAACATCT
1030












AATGGACTTCCACGCGTGGCGGATGT


RB1
NM_000321
9
13
+
74
−
0
37
1
0
CGCGAATGCCTAATATTTTATTAAATTTCCTTTC
1031












AGATTACCTCTACGCGTGGCGGATGT


RET
NM_020630
5
10
+
0
+
0
37
1
0
CGCGAATGCCGACACCGTGGTGGCCACGCTGCG
1032












TGTCTTCGATGCACGCGTGGCGGATGT


RET
NM_020630
5
10
+
35
−
0
37
1
0
CGCGAATGCCGCCTCACCAGCTCCCCTGATGCA
1033












GGTACCACGTCTACGCGTGGCGGATGT


RET
NM_020630
5
10
+
70
+
0
37
1
0
CGCGAATGCCGGTACACAAGCACGCTGCTCCCC
1034












GGGGACACCTGGACGCGTGGCGGATGT


RET
NM_020630
5
10
+
105
−
0
37
1
0
CGCGAATGCCTTGGGCCAGTGTTCCACCCGGAA
1035












GGTCTGCTGGGCACGCGTGGCGGATGT


RET
NM_020630
5
10
+
140
+
0
37
1
0
CGCGAATGCCCGAGACCTCGGTCCAGGCCAACG
1036












GCAGCTTCGTGCACGCGTGGCGGATGT


RET
NM_020630
5
10
+
175
−
0
37
1
0
CGCGAATGCCCCAGCCCCTCTTACTATAGTCATG
1037












TACGGTCGCCCACGCGTGGCGGATGT


PKN1
NM_002741
22
19
+
0
+
0
37
1
0
CGCGAATGCCACTCTGGGCTGGGAAGCCCTGTT
1038












GGCCCGGCGCCTACGCGTGGCGGATGT


PKN1
NM_002741
22
19
+
35
−
0
37
1
0
CGCGAATGCCTGCGGCCGGACAGCGTGGGCACA
1039












AAGGGCGGTGGCACGCGTGGCGGATGT


PKN1
NM_002741
22
19
+
70
+
0
37
1
0
CGCGAATGCCCCGACGTCAGCAACTTCGACGAG
1040












GAGTTCACCGGGACGCGTGGCGGATGT


PKN1
NM_002741
22
19
+
105
−
0
37
1
0
CGCGAATGCCCGCGCGTCGCGGGGCGGGCTCAG
1041












TGTGGGGGCCTCACGCGTGGCGGATGT


PKN1
NM_002741
22
19
+
140
+
0
37
1
0
CGCGAATGCCGCCCCTCACAGCCGCGGAGCAGG
1042












CAGCCTTCCTGGACGCGTGGCGGATGT


PKN1
NM_002741
22
19
+
171
−
3
37
1
0
CGCGAATGCCGGCTAGCAGCCCCCGGCCACGAA
1043












GTCGAAGTCCAGACGCGTGGCGGATGT


PIK3CA
NM_006218
13
3
+
−18
+
0
23
1
1
CGCGAATGCCTTTTTTGGAATCACCTAGGTCCTA
1044












AAATATGAACAACGCGTGGCGGATGT


PIK3CA
NM_006218
13
3
+
17
−
0
0
2
0
CGCGAATGCCTCAGTAAAAATCTCACAAGCAAG
1045












TTATCCAAATATACGCGTGGCGGATGT


PIK3CA
NM_006218
13
3
+
52
+
0
0
2
0
CGCGAATGCCAGAAAGCATTGACTAATCAAAGG
1046












ATTGGGCACTTTACGCGTGGCGGATGT


PIK3CA
NM_006218
13
3
+
87
−
0
0
2
0
CGCGAATGCCGAAAATAATTAGACTTACTTTAA
1047












ATGCCAAAAGAAACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
0
+
0
37
1
0
CGCGAATGCCGGCTGAGCCTAATCCTCTGCCAG
1048












CTTTCATTACCCACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
35
−
0
37
1
0
CGCGAATGCCTGCACAACCTTTTCATTTTCATTT
1049












GGAAGGATAGAACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
70
+
0
37
1
0
CGCGAATGCCGCTGAATTCATCCTTTTCTCTGAG
1050












ATGCTTTGGGGACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
105
−
0
37
1
0
CGCGAATGCCTTCAGACATGGGGTACTGCCAGC
1051












TCACTTCACTCTACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
140
+
0
37
1
0
CGCGAATGCCGAAGAGAGCTCCGATGTGGAAAT
1052












CAGAAATGAAGAACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
175
−
0
37
1
0
CGCGAATGCCCTTCCAAGACCGTCACAAAAAGG
1053












CCGCTGTTGTTTACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
210
+
0
37
1
0
CGCGAATGCCTGAGCAGTGCCTCGGCGGCCCAC
1054












ACAGGGTTGTACACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
245
−
0
37
1
0
CGCGAATGCCTTCTCTTCTGTCTGAGTGTGGTTG
1055












TAATAGCAAGTACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
280
+
0
37
1
0
CGCGAATGCCTGAGCTTGAAGGCAGGCACATTT
1056












ACATCTATGTGCACGCGTGGCGGATGT


PDGFRA
NM_006206
3
4
+
315
−
0
37
1
0
CGCGAATGCCGAAGCTTGGTCCTGGAGACCCAG
1057












CCAACTCACCTGACGCGTGGCGGATGT


EPHA7
NM_004440
16
6
−
−38
+
0
37
1
0
CGCGAATGCCTTTCATGTAACATATGGAAATAC
1058












AACTTTCTTTTTACGCGTGGCGGATGT


EPHA7
NM_004440
16
6
−
−3
−
0
37
1
0
CGCGAATGCCTGTTTGTTGTGCTTTAGAATCCAG
1059












CAGTAGTACTGACGCGTGGCGGATGT


EPHA7
NM_004440
16
6
−
32
+
0
37
1
0
CGCGAATGCCGAGTTGGAGTGGATTTCCTCTCC
1060












ACCCAATGGGGTACGCGTGGCGGATGT


EPHA7
NM_004440
16
6
−
67
−
0
37
1
0
CGCGAATGCCTTTAAAAAAGTGATATTTTATGA
1061












TGAAAAAAACTTACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
0
+
0
37
1
0
CGCGAATGCCAATGAAGAGGTCCATGATGAGGC
1062












CGAAGAGTCAGAACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
35
−
0
37
1
0
CGCGAATGCCAGAGGAGGGACAGGTTCATCTCC
1063












TCGAAGTCATCCACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
70
+
0
37
1
0
CGCGAATGCCCGGCCCGGAGCTTCCCACGCAAG
1064












GCCAGCCAGACCACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
105
−
0
37
1
0
CGCGAATGCCTCAAAGGGGATGTCCCACTCCTG
1065












AAGGAAGATGCTACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
140
+
0
37
1
0
CGCGAATGCCGCAGCTGGAGATCGGCGAGCTCA
1066












TTGGAAAGGGCCACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
175
−
0
37
1
0
CGCGAATGCCGCCATGCCAGCGGCCGTGGTACA
1067












CTTGCCCAAAGCACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
210
+
0
37
1
0
CGCGAATGCCGAGGTGGCCATCCGGCTGATTGA
1068












CATTGAGAGGGAACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
245
−
0
37
1
0
CGCGAATGCCCCTCCCGCTTGAAGGCCTTGAGC
1069












TGGTCCTCGTTGACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
280
+
0
37
1
0
CGCGAATGCCTGATGGCCTACAGGCAGACACGG
1070












CATGAGAACGTGACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
315
−
0
37
1
0
CGCGAATGCCTGAGGCGGGCTCATGCAGGCACC
1071












CATGAAAAGCACACGCGTGGCGGATGT


KSR2
NM_173598
7
12
−
350
+
0
37
1
0
CGCGAATGCCCCTGGCCATCATCACCAGGTCAG
1072












TTCCACCTGGGCACGCGTGGCGGATGT


GUCY2F
NM_001522
9
X
−
0
+
0
37
1
0
CGCGAATGCCAAATCATAAACAGACTTAAGAAG
1073












CCTCCTCCTGTGACGCGTGGCGGATGT


GUCY2F
NM_001522
9
X
−
35
−
0
37
1
0
CGCGAATGCCGGAGGGGCATGCTCAGGAGGAA
1074












CTACTGGTCTGTAACGCGTGGCGGATGT


GUCY2F
NM_001522
9
X
−
70
+
0
37
1
0
CGCGAATGCCAGAATGTCTCCAGCTGATGAAGC
1075












AGTGCTGGGCTGACGCGTGGCGGATGT


GUCY2F
NM_001522
9
X
−
105
−
0
37
1
0
CGCGAATGCCTATTTCATCAAAAGTTGGTCGTTG
1076












TTCTGCAGCCTACGCGTGGCGGATGT


GUCY2F
NM_001522
9
X
−
140
+
0
37
1
0
CGCGAATGCCTTTAACCAGGTAAGGACTCTGAA
1077












TCTTATCATTGCACGCGTGGCGGATGT


RBBP8
NM_002894
6
18
+
−36
+
0
37
1
0
CGCGAATGCCTATATTATTTGCCTTCTTTTTCAC
1078












ATTGTTTTTAAACGCGTGGCGGATGT


RBBP8
NM_002894
6
18
+
−1
−
0
37
1
0
CGCGAATGCCTTATTTTCTTCCTGTAGAGTATTC
1079












CTTTCATTCACACGCGTGGCGGATGT


RBBP8
NM_002894
6
18
+
34
+
0
37
1
0
CGCGAATGCCAAAGCTTTCTGAACAACTCCAGC
1080












AGAAAATTGAGTACGCGTGGCGGATGT


RBBP8
NM_002894
6
18
+
69
−
0
37
1
0
CGCGAATGCCATAAAATAAAATAACAAGGTTGG
1081












AGGAAAATACTTACGCGTGGCGGATGT


RB1
NM_000321
1
13
+
−6
+
2
37
1
0
CGCGAATGCCGGCGTCATGCCGCCCAAAACCCC
1082












CCGAAAAACGGCACGCGTGGCGGATGT


RB1
NM_000321
1
13
+
33
−
35
37
1
0
CGCGAATGCCGGTGCCGGGGGTTCCGCGGCGGC
1083












AGCGGCGGCGGTACGCGTGGCGGATGT


RB1
NM_000321
1
13
+
72
+
8
37
1
0
CGCGAATGCCCCGCCGCCCCCTCCTGAGGAGGA
1084












CCCAGAGCAGGAACGCGTGGCGGATGT


RB1
NM_000321
1
13
+
103
−
0
37
1
0
CGCGAATGCCCCTGACGAGAGGCAGGTCCTCCG
1085












GGCCGCTGTCCTACGCGTGGCGGATGT


PDGFRA
NM_006206
14
4
+
−14
+
0
37
1
0
CGCGAATGCCTATTCTTTCAACAGCCACGGCCA
1086












GATCCAGTGAAAACGCGTGGCGGATGT


PDGFRA
NM_006206
14
4
+
21
−
0
37
1
0
CGCGAATGCCAGTCATTATCTTCAGTTCAGACAT
1087












GAGAGCTTGTTACGCGTGGCGGATGT


PDGFRA
NM_006206
14
4
+
56
+
0
37
1
0
CGCGAATGCCCACCTGGGGCCACATTTGAACAT
1088












TGTAAACTTGCTACGCGTGGCGGATGT


PDGFRA
NM_006206
14
4
+
91
−
0
37
1
0
CGCGAATGCCGGTCAGTGAGCCCACCTGACTTG
1089












GTGCAGGCTCCCACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
0
+
0
37
1
0
CGCGAATGCCGATCAGGAGCGTCTGGGCAAGCA
1090












GCTCAAGTTACGACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
35
−
0
37
1
0
CGCGAATGCCCCTCTTTCAGCTTCTCCTTTTCTTC
1091












CCTTTCTGCAACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
66
+
4
37
1
0
CGCGAATGCCGAGGAGGCCAAGCGGGCCAAGG
1092












AGGAGGCCAAGAAACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
105
−
35
37
1
0
CGCGAATGCCCTCTCCTTTTCCTTAAGCTCCTTC
1093












TCTTCCTCCTTACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
144
+
31
37
1
0
CGCGAATGCCGAGAAGCGGGAGAAGGATGAGA
1094












AGGAGAAGGCGGAACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
175
−
0
37
1
0
CGCGAATGCCCTTGCGCCGCTCCTCCTTGAGCCG
1095












CTGCTTCTCCGACGCGTGGCGGATGT


CHAF1A
NM_005483
5
19
+
210
+
0
37
1
0
CGCGAATGCCGAGAGACAGGAAGCCCTGGAGT
1096












GAGTGTCCTTGGAACGCGTGGCGGATGT


NFKB1
NM_003998
2
4
+
−54
+
17
37
1
0
CGCGAATGCCGTTCATTCTAGTGTTACAGTTTTG
1097












TTTTGTTTTGTACGCGTGGCGGATGT


NFKB1
NM_003998
2
4
+
−15
−
0
37
1
0
CGCGAATGCCTATGGATCATCTTCTGCCATTCTG
1098












AAGCTGTGTATACGCGTGGCGGATGT


NFKB1
NM_003998
2
4
+
20
+
0
37
1
0
CGCGAATGCCTTTGGGAAGGCCTGAACAAGTAA
1099












GTGTCATAATCTACGCGTGGCGGATGT


NFKB1
NM_003998
2
4
+
55
−
0
37
1
0
CGCGAATGCCTGAAATATGAATATATTTAAATA
1100












AAGTTATCAGTGACGCGTGGCGGATGT


RET
NM_020630
11
10
+
0
+
0
37
1
0
CGCGAATGCCATCCACTGTGCGACGAGCTGTGC
1101












CGCACGGTGATCACGCGTGGCGGATGT


RET
NM_020630
11
10
+
35
−
0
37
1
0
CGCGAATGCCACCGAGACGATGAAGGAGAAGA
1102












GGACAGCGGCTGCACGCGTGGCGGATGT


RET
NM_020630
11
10
+
70
+
0
37
1
0
CGCGAATGCCGCTGCTGTCTGCCTTCTGCATCCA
1103












CTGCTACCACAACGCGTGGCGGATGT


RET
NM_020630
11
10
+
105
−
0
37
1
0
CGCGAATGCCCTCAGCTGAGGAGATGGGTGGCT
1104












TGTGGGCAAACTACGCGTGGCGGATGT


RET
NM_020630
11
10
+
140
+
0
37
1
0
CGCGAATGCCATGACCTTCCGGAGGCCCGCCCA
1105












GGCCTTCCCGGTACGCGTGGCGGATGT


RET
NM_020630
11
10
+
175
−
0
37
1
0
CGCGAATGCCGCGAGGGCCGGCGGGCACCGGA
1106












AGAGGAGTAGCTGACGCGTGGCGGATGT


RET
NM_020630
11
10
+
210
+
0
37
1
0
CGCGAATGCCTGGACTCCATGGAGAACCAGGTC
1107












TCCGTGGATGCCACGCGTGGCGGATGT


RET
NM_020630
11
10
+
245
−
0
37
1
0
CGCGAATGCCCCCTGCCCCGCAGGGACCCTCAC
1108












CAGGATCTTGAAACGCGTGGCGGATGT


NFKB1
NM_003998
15
4
+
0
+
0
37
1
0
CGCGAATGCCATAACCTCTTTCTAGAGAAGGCT
1109












ATGCAGCTTGCAACGCGTGGCGGATGT


NFKB1
NM_003998
15
4
+
35
−
0
37
1
0
CGCGAATGCCACCGCGTAGTCGAAAAGGGCATT
1110












GGCATGCCTCTTACGCGTGGCGGATGT


NFKB1
NM_003998
15
4
+
70
+
0
37
1
0
CGCGAATGCCGACAGGAGACGTGAAGATGCTGC
1111












TGGCCGTCCAGCACGCGTGGCGGATGT


NFKB1
NM_003998
15
4
+
105
−
0
37
1
0
CGCGAATGCCGTCCCCATTCTCATCCTGCACAGC
1112












AGTGAGATGGCACGCGTGGCGGATGT


NFKB1
NM_003998
15
4
+
140
+
0
37
1
0
CGCGAATGCCAGGTAAGTCAGAACTTTTGCATG
1113












ATAGGTTGTCCTACGCGTGGCGGATGT


NTRK3
NM_001012338
16
15
−
−34
+
0
37
1
0
CGCGAATGCCGAGCTCTGCTGATCCTCTTTTTCT
1114












CTCTGTCTAGGACGCGTGGCGGATGT


NTRK3
NM_001012338
16
15
−
1
−
0
37
1
0
CGCGAATGCCGGCTGAATGCTCCGAAGTCCTGA
1115












GTTCTTGATGGTACGCGTGGCGGATGT


NTRK3
NM_001012338
16
15
−
36
+
0
37
1
0
CGCGAATGCCCAGAGCCTTTGCCAAGAACCCCC
1116












ATTTGCGTTATAACGCGTGGCGGATGT


NTRK3
NM_001012338
16
15
−
71
−
0
37
1
0
CGCGAATGCCGTGCCCCCAATCCCTGCAGCCCA
1117












GCTCTACTCACAACGCGTGGCGGATGT


NFKB1
NM_003998
9
4
+
−18
+
0
37
1
0
CGCGAATGCCTTTTTCCCCTGTGAACAGAAGCC
1118












CCCAATGCATCCACGCGTGGCGGATGT


NFKB1
NM_003998
9
4
+
17
−
0
37
1
0
CGCGAATGCCCCAGCTGTCCTGTCCATTCTTACA
1119












ATTTTCAAGTTACGCGTGGCGGATGT


NFKB1
NM_003998
9
4
+
52
+
0
37
1
0
CGCGAATGCCATGTGTGACTGGAGGGGAGGAA
1120












ATTTATCTTCTTTACGCGTGGCGGATGT


NFKB1
NM_003998
9
4
+
87
−
0
37
1
0
CGCGAATGCCTCACAGAATGTATTTACCTTTCTG
1121












AACTTTGTCACACGCGTGGCGGATGT


CENTG1
NM_014770
13
12
−
−2
+
0
37
1
0
CGCGAATGCCAGACAGGATCAGTGCTTCCTCCC
1122












CTCGGGTGGTGGACGCGTGGCGGATGT


CENTG1
NM_014770
13
12
−
33
−
0
37
1
0
CGCGAATGCCCATGTCCGCGCACAGAGCTCTGG
1123












CACGAGCATCTCACGCGTGGCGGATGT


CENTG1
NM_014770
13
12
−
68
+
0
37
1
0
CGCGAATGCCAAACGCTGCAGCTACTATGAGAC
1124












TTGTGCAACCTAACGCGTGGCGGATGT


CENTG1
NM_014770
13
12
−
103
−
0
37
1
0
CGCGAATGCCCACCCTCCTGGAAGACCCGATCC
1125












ACATTGAGCCCAACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
0
+
0
37
1
0
CGCGAATGCCTGGGAGGAAGTGAGTATCATGGA
1126












TGAAAAAAATACACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
35
−
0
37
1
0
CGCGAATGCCCCATCACATTGCACACTTGGTAG
1127












GTTCGGATTGGTACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
70
+
0
37
1
0
CGCGAATGCCAACCCAGCCAGAATAACTGGCTA
1128












CGAACTGATTGGACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
105
−
0
37
1
0
CGCGAATGCCTCAATATACACCCTCTGAGCCCC
1129












TTCTCGGGTGATACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
140
+
0
37
1
0
CGCGAATGCCGATTAAATTCACCTTGAGGGACT
1130












GCAATAGTCTTCACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
175
−
0
37
1
0
CGCGAATGCCGTTAAACGTCTCCTTGCAAGTCC
1131












CCATGACGCCCGACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
210
+
0
37
1
0
CGCGAATGCCCTGTACTACTATGAATCAGACAA
1132












CGACAAAGAGCGACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
245
−
0
37
1
0
CGCGAATGCCTGTCAATTTTGACAAACTGGTTCT
1133












CTCTGATGAAAACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
280
+
0
37
1
0
CGCGAATGCCCCATTGCTGCTGATGAGAGCTTC
1134












ACCCAAGTGGACACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
315
−
0
37
1
0
CGCGAATGCCATCTCGGTGTTCAGCTTCATGATT
1135












CTGTCACCAATACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
350
+
0
37
1
0
CGCGAATGCCCCGGGATGTAGGGCCATTAAGCA
1136












AAAAGGGGTTTTACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
385
−
0
37
1
0
CGCGAATGCCGGCGATGCAGGCCCCCACATCCT
1137












GAAAAGCCAGGTACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
420
+
0
37
1
0
CGCGAATGCCCTGGTATCAGTCCGTGTGTTCTAT
1138












AAAAAGTGTCCACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
455
−
0
37
1
0
CGCGAATGCCTGTCAGGAAACTGGGCCAGATTG
1139












CGGACTGTGAGTACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
490
+
0
37
1
0
CGCGAATGCCCCATCACAGGGGCTGATACGTCT
1140












TCCCTGGTGGAAACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
525
−
0
37
1
0
CGCGAATGCCTTCTCTTCTGAGTTGTTGACACAG
1141












GAGCCTCGAACACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
560
+
0
37
1
0
CGCGAATGCCAGATGTGCCAAAAATGTACTGTG
1142












GGGCAGATGGTGACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
595
−
0
37
1
0
CGCGAATGCCGTTGCATAGGCAGTTGCCAATGG
1143












GTACCAGCCATTACGCGTGGCGGATGT


EPHA4
NM_004438
16
2
−
630
+
0
37
1
0
CGCGAATGCCGCTGGGCATGAGGAGCGGAGCG
1144












GAGAATGCCAAGGACGCGTGGCGGATGT


CENTG1
NM_014770
12
12
−
−15
+
0
37
1
0
CGCGAATGCCGTCAACTCCCCTCAGTGGCCCAG
1145












AAGGTGGTGACCACGCGTGGCGGATGT


CENTG1
NM_014770
12
12
−
20
−
0
37
1
0
CGCGAATGCCTTGCAGGCAGCCAGAAGCTGTTG
1146












CTGCTTGCGCAAACGCGTGGCGGATGT


CENTG1
NM_014770
12
12
−
55
+
0
37
1
0
CGCGAATGCCGTCCCTGCCCAGCTCCCCAAGCC
1147












ACTCAGCTGCATACGCGTGGCGGATGT


CENTG1
NM_014770
12
12
−
90
−
0
37
1
0
CGCGAATGCCGAAACCCCAACTCACCTGGCCAG
1148












CTACCGGAGTGGACGCGTGGCGGATGT


PKN1
NM_002741
16
19
+
0
+
0
37
1
0
CGCGAATGCCCCTGATGTGTGAGAAGCGGATAT
1149












TGGCGGCAGTGAACGCGTGGCGGATGT


PKN1
NM_002741
16
19
+
35
−
0
37
1
0
CGCGAATGCCGAAGAGGTTCACCAGGAAGGGG
1150












TGTCCCGCACTGGACGCGTGGCGGATGT


PKN1
NM_002741
16
19
+
70
+
0
37
1
0
CGCGAATGCCGGCTGTTTCCAGACACCGGAGCA
1151












CGTGTGCTTCGTACGCGTGGCGGATGT


PKN1
NM_002741
16
19
+
105
−
0
37
1
0
CGCGAATGCCGCAGCATCAGGTCCCCACCGGCC
1152












GAGTACTCCATCACGCGTGGCGGATGT


PKN1
NM_002741
16
19
+
140
+
0
37
1
0
CGCGAATGCCACATCCACAGCGACGTGTTCTCT
1153












GAGCCCCGTGCCACGCGTGGCGGATGT


PKN1
NM_002741
16
19
+
175
−
0
37
1
0
CGCGAATGCCGAGGCAGGGGCCCGGGCTGGGG
1154












TCCAGGCTCACATACGCGTGGCGGATGT


GUCY2F
NM_001522
8
X
−
0
+
0
37
1
0
CGCGAATGCCTTTAAAACTTTTAATAAAGGGAA
1155












GAAGACCAATATACGCGTGGCGGATGT


GUCY2F
NM_001522
8
X
−
35
−
0
37
1
0
CGCGAATGCCAATATTGCTCCAACATCCGAAGC
1156












ATAGAATCAATAACGCGTGGCGGATGT


GUCY2F
NM_001522
8
X
−
70
+
0
37
1
0
CGCGAATGCCCTAGCAACTTGGAAGATTTGATT
1157












CGGGAGCGGACTACGCGTGGCGGATGT


GUCY2F
NM_001522
8
X
−
105
−
0
37
1
0
CGCGAATGCCTTTTCCGTTTTCTGTTTTTCAATTT
1158












CCAGCTCTTCACGCGTGGCGGATGT


GUCY2F
NM_001522
8
X
−
140
+
0
37
1
0
CGCGAATGCCGCTTCTAACACAGATGCTACCAC
1159












CGTATGTGAGAAACGCGTGGCGGATGT


RET
NM_020630
19
10
+
0
+
0
37
1
0
CGCGAATGCCGACTACTTGGACCTTGCGGCGTC
1160












CACTCCATCTGAACGCGTGGCGGATGT


RET
NM_020630
19
10
+
35
−
0
37
1
0
CGCGAATGCCCCTCCTCTGAGAGGCCGTCGTCA
1161












TAAATCAGGGAGACGCGTGGCGGATGT


RET
NM_020630
19
10
+
70
+
0
37
1
0
CGCGAATGCCAGACACCGCTGGTGGACTGTAAT
1162












AATGCCCCCCTCACGCGTGGCGGATGT


RET
NM_020630
19
10
+
105
−
0
37
1
0
CGCGAATGCCTTGTTTTCAATCCATGTGGAAGG
1163












GAGGGCTCGAGGACGCGTGGCGGATGT


RET
NM_020630
19
10
+
140
+
0
37
1
0
CGCGAATGCCACTCTATGGTAGAATTTCCCATG
1164












CATTTACTAGATACGCGTGGCGGATGT


RET
NM_020630
19
10
+
175
−
0
37
1
0
CGCGAATGCCAGGAAGGATAGTGCAAAGGGGA
1165












CAGCGGTGCTAGAACGCGTGGCGGATGT


RB1
NM_000321
12
13
+
−26
+
0
37
1
0
CGCGAATGCCCATTTTCCTATTTTTATCCCCTCT
1166












AGGACTGTTATACGCGTGGCGGATGT


RB1
NM_000321
12
13
+
9
−
0
37
1
0
CGCGAATGCCAATTTAAAATCATCATTAATTGTT
1167












GGATAGTGTTCACGCGTGGCGGATGT


RB1
NM_000321
12
13
+
44
+
0
37
1
0
CGCGAATGCCCAGCAAGTGATCAACCTTCAGAA
1168












AATCTGATTTCCACGCGTGGCGGATGT


RB1
NM_000321
12
13
+
79
−
0
37
1
0
CGCGAATGCCTAAATAATGTTTCATATATGGCTT
1169












ACGTTAAAATAACGCGTGGCGGATGT


GUCY2F
NM_001522
14
X
−
−4
+
0
37
1
0
CGCGAATGCCACAGAGAGGAAGTCGTGCCAGTG
1170












TAAGCTTCCAGAACGCGTGGCGGATGT


GUCY2F
NM_001522
14
X
−
31
−
0
37
1
0
CGCGAATGCCTCTTGGGGACCTCCCACTTTGGA
1171












CCTCTGAGGTAAACGCGTGGCGGATGT


GUCY2F
NM_001522
14
X
−
66
+
0
37
1
0
CGCGAATGCCCTCTCCTTTTCTTCAGGGAGTCTA
1172












ACTCCAGCTACACGCGTGGCGGATGT


GUCY2F
NM_001522
14
X
−
101
−
0
37
1
0
CGCGAATGCCTTACCTCATAAATCGCTATGTTGG
1173












AGTTTTCATAGACGCGTGGCGGATGT


RET
NM_020975
20
10
+
0
+
0
37
1
0
CGCGAATGCCGCATGTCAGACCCGAACTGGCCT
1174












GGAGAGAGTCCTACGCGTGGCGGATGT


RET
NM_020975
20
10
+
35
−
0
37
1
0
CGCGAATGCCCCAGTGTTAGTGCCATCAGCTCT
1175












CGTGAGTGGTACACGCGTGGCGGATGT


RET
NM_020975
20
10
+
70
+
0
37
1
0
CGCGAATGCCGTTTCCAAGATATCCAAATGATA
1176












GTGTATATGCTAACGCGTGGCGGATGT


RET
NM_020975
20
10
+
105
−
0
37
1
0
CGCGAATGCCCATTAATTTTGCCGCTGAGGGTG
1177












AAAGCATCCAGTACGCGTGGCGGATGT


RET
NM_020975
20
10
+
140
+
0
37
1
0
CGCGAATGCCGACACGTTTGATAGTTAACATTT
1178












CTTTGTGAAAGGACGCGTGGCGGATGT


CHAF1A
NM_005483
6
19
+
−40
+
0
37
1
0
CGCGAATGCCAAGCAAAGAGTCGGCTGAAATGT
1179












CATTTGCTGTCTACGCGTGGCGGATGT


CHAF1A
NM_005483
6
19
+
−5
−
0
37
1
0
CGCGAATGCCTCCTTTTTCCTTTTTTCCTCAAGTT
1180












TAGCCCTGTGACGCGTGGCGGATGT


CHAF1A
NM_005483
6
19
+
30
+
0
37
1
0
CGCGAATGCCAGAAGAGAAACGGTTAAGAGAA
1181












GAAGAGAAGGTAGACGCGTGGCGGATGT


CHAF1A
NM_005483
6
19
+
65
−
0
37
1
0
CGCGAATGCCACGGGCTGGGACGGGGAAGCTCT
1182












GTGGGAAACACTACGCGTGGCGGATGT


KSR2
NM_173598
14
12
−
−28
+
0
37
1
0
CGCGAATGCCTTGAACATTTTCTGTCTCTTTTCC
1183












ACAGGTTTTCCACGCGTGGCGGATGT


KSR2
NM_173598
14
12
−
7
−
0
37
1
0
CGCGAATGCCCAGACTGTGCACGTCTGAGACAT
1184












CCAGTACTTGGTACGCGTGGCGGATGT


KSR2
NM_173598
14
12
−
42
+
0
37
1
0
CGCGAATGCCTGGGAAAGGGATGCTTTTTGGCC
1185












TCAAGTGTAAAAACGCGTGGCGGATGT


KSR2
NM_173598
14
12
−
77
−
0
37
1
0
CGCGAATGCCAAAGTCACTGCAGGGCACAGTCA
1186












CTTACTTGCAGTACGCGTGGCGGATGT


EPHA7
NM_004440
9
6
−
−42
+
0
37
1
0
CGCGAATGCCAAGTAAAATGACTGAGATTGTCA
1187












CAAATTTGCTTTACGCGTGGCGGATGT


EPHA7
NM_004440
9
6
−
−7
−
0
37
1
0
CGCGAATGCCTTGGTCAGCTTTGCTATAACCAC
1188












AGTGCCTTGAAGACGCGTGGCGGATGT


EPHA7
NM_004440
9
6
−
28
+
0
37
1
0
CGCGAATGCCGAAGGCGATGAAGAGCTTTACTT
1189












TCATTGTAAGTGACGCGTGGCGGATGT


EPHA7
NM_004440
9
6
−
63
−
0
37
1
0
CGCGAATGCCTTAATAGGGGTACATCATAATAA
1190












AGAAAAGCCAAAACGCGTGGCGGATGT


RPS6KA1
NM_002953
10
1
+
−34
+
0
37
1
0
CGCGAATGCCGATCAGAGCCTGAATAGATCCTT
1191












GTCCTCTGCAGTACGCGTGGCGGATGT


RPS6KA1
NM_002953
10
1
+
1
−
0
37
1
0
CGCGAATGCCCCCCTGGAAGGGCAGGGAGCCCG
1192












TCAGCATCTCAAACGCGTGGCGGATGT


RPS6KA1
NM_002953
10
1
+
36
+
0
37
1
0
CGCGAATGCCAAGGACCGGAAGGAGACCATGA
1193












CACTGATTCTGAAACGCGTGGCGGATGT


RPS6KA1
NM_002953
10
1
+
71
−
0
37
1
0
CGCGAATGCCGAGTCCATTGTTATCAGGGCAGG
1194












GCTGGGGCTTACACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
0
+
0
37
1
0
CGCGAATGCCGACCTCCATCTTCACCAAGAAAT
1195












GTTATCTCTAATACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
35
−
0
37
1
0
CGCGAATGCCCAACTCCAGTCCAGGATAACTGA
1196












GGTCTCGTTTATACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
70
+
0
37
1
0
CGCGAATGCCGCCCCTGGACACAGGAGGCCGGA
1197












AAGATGTTACCTACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
105
−
0
37
1
0
CGCGAATGCCTATATTCCACCCACATTTTTTACA
1198












TATGATGTTGAACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
140
+
0
37
1
0
CGCGAATGCCAAACAGTGTGAGCCATGCAGCCC
1199












AAATGTCCGCTTACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
175
−
0
37
1
0
CGCGAATGCCCCGTGGTGTTGGTGAGTCCAAAC
1200












TGTCGAGGGAGGACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
210
+
0
37
1
0
CGCGAATGCCTGACAGTGACAGACCTTCTGGCA
1201












CATACTAACTACACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
245
−
0
37
1
0
CGCGAATGCCTCTGACACCCCATTAACGGCATC
1202












AATCTCAAAGGTACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
280
+
0
37
1
0
CGCGAATGCCGCTGAGCTCCCCACCAAGACAGT
1203












TTGCTGCGGTCAACGCGTGGCGGATGT


EPHA3
NM_005233
5
3
+
315
−
0
37
1
0
CGCGAATGCCTAGTATGTACTCACCAGCCTGAT
1204












TAGTTGTGATGCACGCGTGGCGGATGT


RET
NM_020630
10
10
+
−10
+
0
37
1
0
CGCGAATGCCCTGCCCTCAGGGGGCAGCATTGT
1205












TGGGGGACACGAACGCGTGGCGGATGT


RET
NM_020630
10
10
+
25
−
0
37
1
0
CGCGAATGCCCATAGCCAGCTTTAATCCCCCGG
1206












GGCTCCCCAGGCACGCGTGGCGGATGT


RET
NM_020630
10
10
+
60
+
0
37
1
0
CGCGAATGCCGCACCTGCAACTGCTTCCCTGAG
1207












GAGGAGAAGTGCACGCGTGGCGGATGT


RET
NM_020630
10
10
+
95
−
0
37
1
0
CGCGAATGCCACCCACTCACCCTGGATGTCTTC
1208












GGGCTCGCAGAAACGCGTGGCGGATGT


NFKB1
NM_003998
13
4
+
−25
+
0
37
1
0
CGCGAATGCCTCATTTGTTTTACTTGCCGTTTCA
1209












GGGTATAGCTTACGCGTGGCGGATGT


NFKB1
NM_003998
13
4
+
10
−
0
37
1
0
CGCGAATGCCTAATCCCACCATAAGTAGGAAAT
1210












CCATAGTGTGGGACGCGTGGCGGATGT


NFKB1
NM_003998
13
4
+
45
+
0
37
1
0
CGCGAATGCCCTTTCCATCCTGGAACTACTAAAT
1211












CTAATGCTGGGACGCGTGGCGGATGT


NFKB1
NM_003998
13
4
+
80
−
0
37
1
0
CGCGAATGCCGTATTAAGAACAAAGCATTACTT
1212












ACCATGCTTCATACGCGTGGCGGATGT


RB1
NM_000321
18
13
+
−10
+
0
37
1
0
CGCGAATGCCTTTCATATAGGATTCACCTTTATT
1213












TGATCTTATTAACGCGTGGCGGATGT


RB1
NM_000321
18
13
+
25
−
0
37
1
0
CGCGAATGCCGTGATCAGTTGGTCCTTCTCGGTC
1214












CTTTGATTGTTACGCGTGGCGGATGT


RB1
NM_000321
18
13
+
60
+
0
37
1
0
CGCGAATGCCCTTGAATCTGCTTGTCCTCTTAAT
1215












CTTCCTCTCCAACGCGTGGCGGATGT


RB1
NM_000321
18
13
+
95
−
0
37
1
0
CGCGAATGCCATTTTGCTTACATATCTGCTGCAG
1216












TGTGATTATTCACGCGTGGCGGATGT


GUCY2F
NM_001522
5
X
−
−20
+
0
37
1
0
CGCGAATGCCAACCCCCTGATACTCTATAGGGC
1217












CGGTTGTTGCTGACGCGTGGCGGATGT


GUCY2F
NM_001522
5
X
−
15
−
0
37
1
0
CGCGAATGCCCAAGCAGTATCTGGGCATGGTGA
1218












GGCCCACCACTCACGCGTGGCGGATGT


GUCY2F
NM_001522
5
X
−
50
+
0
37
1
0
CGCGAATGCCTTTGGAGACACTGTGAACACAGC
1219












TTCTCGGATGGAACGCGTGGCGGATGT


GUCY2F
NM_001522
5
X
−
85
−
0
37
1
0
CGCGAATGCCATCTCTCTATTAGGTACTCACGTA
1220












AGCCTGTAGATACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
0
+
0
37
1
0
CGCGAATGCCAAACATGATGGGCAATTTACAGT
1221












CATTCAGTTAGTACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
35
−
0
37
1
0
CGCGAATGCCATCTCATTCCAGCAGCAATTCCTC
1222












TCAGCATTCCTACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
70
+
0
37
1
0
CGCGAATGCCATTTGGCTGATATGGGATATGTT
1223












CACAGGGACCTTACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
105
−
0
37
1
0
CGCGAATGCCACGAGATTGCTGTTGACAAGAAT
1224












ATTGCGAGCTGCACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
140
+
0
37
1
0
CGCGAATGCCTTGTAAAGTGTCAGATTTTGGCCT
1225












GTCCCGAGTTAACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
175
−
0
37
1
0
CGCGAATGCCAGTAGTTGTATAGACAGCTTCTG
1226












GATCATCCTCTAACGCGTGGCGGATGT


EPHA7
NM_004440
5
6
−
210
+
0
37
1
0
CGCGAATGCCGTAAGAAAAAGTCATTTACTGCA
1227












CTTCTTCATATTACGCGTGGCGGATGT


NFKB1
NM_003998
24
4
+
0
+
0
37
1
0
CGCGAATGCCACGAGCTCCGAGACAGTGACAGT
1228












GTCTGCGACAGCACGCGTGGCGGATGT


NFKB1
NM_003998
24
4
+
35
−
0
37
1
0
CGCGAATGCCGTAAAGCTGAGTTTGCGGAAGGA
1229












TGTCTCCACGCCACGCGTGGCGGATGT


NFKB1
NM_003998
24
4
+
70
+
0
37
1
0
CGCGAATGCCCGAGTCTCTGACCAGTGGTGCCT
1230












CACTGCTAACTCACGCGTGGCGGATGT


NFKB1
NM_003998
24
4
+
105
−
0
37
1
0
CGCGAATGCCTCCTTCCTGCCCATAATCATGGG
1231












GCATTTTGTTGAACGCGTGGCGGATGT


NFKB1
NM_003998
24
4
+
140
+
0
37
1
0
CGCGAATGCCCCTCTAGAAGGCAAAATTTAGCC
1232












TGCTGACAATTTACGCGTGGCGGATGT


RBBP8
NM_203292
18
18
+
−18
+
0
37
1
0
CGCGAATGCCTTATGATTTGTTTTTAAGGTTATA
1233












TTAAGGAAGATACGCGTGGCGGATGT


RBBP8
NM_203292
18
18
+
17
−
0
37
1
0
CGCGAATGCCGGCTGACGTCTTTTTGGACGAGG
1234












ACAAGGATCAAGACGCGTGGCGGATGT


RBBP8
NM_203292
18
18
+
52
+
0
37
1
0
CGCGAATGCCTTACAACGCAATATTTTCTCCAA
1235












AAGGCAAGGAGCACGCGTGGCGGATGT


RBBP8
NM_203292
18
18
+
87
−
0
37
1
0
CGCGAATGCCATCCTTCTGTTTCTGTTTCAACGT
1236












CTATGTCTTCTACGCGTGGCGGATGT


EPHA4
NM_004438
2
2
−
−12
+
0
37
1
0
CGCGAATGCCCCCTATGTACAGGGACCTGGCAA
1237












GAATTGGTATCAACGCGTGGCGGATGT


EPHA4
NM_004438
2
2
−
23
−
0
37
1
0
CGCGAATGCCACTGCTCAAAATCTTATTCTGGTG
1238












CGTGATGGCTGACGCGTGGCGGATGT


EPHA4
NM_004438
2
2
−
58
+
0
37
1
0
CGCGAATGCCGTCCAGGCAATGCGAACCCAAAT
1239












GCAGCAGATGCAACGCGTGGCGGATGT


EPHA4
NM_004438
2
2
−
93
−
0
37
1
0
CGCGAATGCCATTCAGTACTGGCTCAGACGGGA
1240












ACCATTCTGCCGACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
0
+
0
37
1
0
CGCGAATGCCTACGTGACCGCGTCCGGACCAAG
1241












ATGGAGAGAGACACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
35
−
0
37
1
0
CGCGAATGCCTTCACCACGAATGGGTGATTTAC
1242












ATCAGCCAGGATACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
70
+
0
37
1
0
CGCGAATGCCGCTGCACTATGGTAAAGCTTCTG
1243












GCCCTGCCTGAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
105
−
0
37
1
0
CGCGAATGCCCCACAGGCAAGGGCGAAGGATG
1244












GGTGGGGTAGGAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
140
+
0
37
1
0
CGCGAATGCCTCTGTACACTGTCCCACCGCCTGC
1245












CTGGCAGGCCAACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
175
−
0
37
1
0
CGCGAATGCCGGCCTTATGATCTGCTTCTCCGGC
1246












CCTGGCTCCCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
210
+
0
37
1
0
CGCGAATGCCGCGCCGACTCTACCATTGCCTTTC
1247












TCCCTCTTCCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
245
−
0
37
1
0
CGCGAATGCCGAATGAGATAGAGCTTGCCCTCG
1248












GTCTGGAAGGCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
280
+
0
37
1
0
CGCGAATGCCTGGACTTCCTGCGTGGTGGGGAC
1249












CTCTTCACCCGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
6
1
+
315
−
0
37
1
0
CGCGAATGCCTCTGGCAGTAGATGTCAGCTCAC
1250












CTCTTTTGAGAGACGCGTGGCGGATGT


NTRK3
NM_001007156
1
15
−
−6
+
2
37
1
0
CGCGAATGCCTTATAGGTTTCAGAGAAATTATG
1251












TTGAATCCAATAACGCGTGGCGGATGT


NTRK3
NM_001007156
1
15
−
25
−
0
37
1
0
CGCGAATGCCGGTTAAGAGGCTTGGAATGTCCG
1252












GGAAGGCTTATTACGCGTGGCGGATGT


NTRK3
NM_001007156
1
15
−
60
+
0
37
1
0
CGCGAATGCCATGGCATCTATGTTGAGGATGTC
1253












AATGTTTATTTCACGCGTGGCGGATGT


NTRK3
NM_001007156
1
15
−
95
−
0
37
1
0
CGCGAATGCCAAAAGGAGTTTTTAAAAGCCATG
1254












ACGTCCTTTGCTACGCGTGGCGGATGT


RET
NM_020630
9
10
+
−14
+
0
37
1
0
CGCGAATGCCTGTGTCCTGTGCAGGGATCACCA
1255












GGAACTTCTCCAACGCGTGGCGGATGT


RET
NM_020630
9
10
+
21
−
0
37
1
0
CGCGAATGCCGCCGTCGGGGCAGGTCTTGGTGC
1256












TGGGAGAGCAGGACGCGTGGCGGATGT


RET
NM_020630
9
10
+
56
+
0
37
1
0
CGCGAATGCCCACTGCGATGTTGTGGAGACCCA
1257












AGACATCAACATACGCGTGGCGGATGT


RET
NM_020630
9
10
+
91
−
0
37
1
0
CGCGAATGCCTTAAACCCTGCTTACGGAGGCAG
1258












TCCTGAGGGCAAACGCGTGGCGGATGT


NTRK3
NM_001012338
3
15
−
−49
+
35
37
1
0
CGCGAATGCCTTTCTAAGTTTTCTTCTAATATTA
1259












TTATTGTTTTGACGCGTGGCGGATGT


NTRK3
NM_001012338
3
15
−
−14
−
35
37
1
0
CGCGAATGCCATTTCCAGATGGATTAAAGAGCT
1260












AAACATAAAAAAACGCGTGGCGGATGT


NTRK3
NM_001012338
3
15
−
21
+
35
37
1
0
CGCGAATGCCGATTTTTGTATATGGTGTGAGGT
1261












AGGTATCTAAGCACGCGTGGCGGATGT


NTRK3
NM_001012338
3
15
−
60
−
32
37
1
0
CGCGAATGCCAAAGTGTTGGGACAATGAACTAT
1262












TCATTAAAAAAAACGCGTGGCGGATGT


EPHB1
NM_004441
9
3
+
−38
+
0
37
1
0
CGCGAATGCCTGCCCTGTGGCTGAGAGAGCCCC
1263












TCTTTTTATCCAACGCGTGGCGGATGT


EPHB1
NM_004441
9
3
+
−3
−
0
37
1
0
CGCGAATGCCTGTACACAGCCTCTTTGCTATAA
1264












GCCCGTTTCCTGACGCGTGGCGGATGT


EPHB1
NM_004441
9
3
+
32
+
0
37
1
0
CGCGAATGCCGCGATAAGCTCCAGCATTACAGC
1265












ACAGGCCGAGGTACGCGTGGCGGATGT


EPHB1
NM_004441
9
3
+
67
−
0
37
1
0
CGCGAATGCCGTGGGGGTCAGACACCGGGTCTC
1266












TGCTTTCTACTTACGCGTGGCGGATGT


PIK3CA
NM_006218
12
3
+
0
+
0
0
2
0
CGCGAATGCCATGTATTGCTTGGTAAAAGATTG
1267












GCCTCCAATCAAACGCGTGGCGGATGT


PIK3CA
NM_006218
12
3
+
35
−
0
0
2
0
CGCGAATGCCAATTACAGTCCAGAAGTTCCATA
1268












GCCTGTTCAGGTACGCGTGGCGGATGT


PIK3CA
NM_006218
12
3
+
70
+
0
37
1
0
CGCGAATGCCACCCAGATCCTATGGTTCGAGGT
1269












TTTGCTGTTCGGACGCGTGGCGGATGT


PIK3CA
NM_006218
12
3
+
103
−
0
37
1
0
CGCGAATGCCAAGTTTGTCATCTGTTAAATATTT
1270












TTCCAAGCACCACGCGTGGCGGATGT


PIK3CA
NM_006218
12
3
+
136
+
5
0
2
0
CGCGAATGCCTTTCTCAGTATTTAATTCAGCTAG
1271












TACAGGTAAAAACGCGTGGCGGATGT


PALB2
NM_024675
5
16
−
0
+
0
37
1
0
CGCGAATGCCGGCATTGTTTTGTTCCTCTGATGA
1272












TGAAAGTGAAAACGCGTGGCGGATGT


PALB2
NM_024675
5
16
−
35
−
0
37
1
0
CGCGAATGCCAGCTTTTATATTTCCAGACTTCAG
1273












TAGTACTTGCTACGCGTGGCGGATGT


PALB2
NM_024675
5
16
−
70
+
0
37
1
0
CGCGAATGCCGTGCTTGGCCTGACAAAGAGGAG
1274












GCTAGTTAGTAGACGCGTGGCGGATGT


PALB2
NM_024675
5
16
−
105
−
0
37
1
0
CGCGAATGCCTGACTTCTACTTGTTGATCAGAA
1275












AGGGTCCCACTGACGCGTGGCGGATGT


PALB2
NM_024675
5
16
−
140
+
0
37
1
0
CGCGAATGCCTGACGTTTGCAGAAGATGGAGGG
1276












TAAGAAAAGCATACGCGTGGCGGATGT


RPS6KA1
NM_002953
18
1
+
0
+
0
37
1
0
CGCGAATGCCGTTGTGCACAGGGACCTGAAGCC
1277












CAGCAACATCCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
18
1
+
35
−
0
37
1
0
CGCGAATGCCGCAGGCACTCGGGATTCCCGGAC
1278












TCGTCCACATACACGCGTGGCGGATGT


RPS6KA1
NM_002953
18
1
+
70
+
0
37
1
0
CGCGAATGCCGCATCTGTGACTTTGGTTTTGCCA
1279












AACAGCTGCGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
18
1
+
105
−
0
37
1
0
CGCGAATGCCGTGTAGCAAGGTGTCATGAGGAG
1280












CCCATTCTCAGCACGCGTGGCGGATGT


RPS6KA1
NM_002953
18
1
+
140
+
0
37
1
0
CGCGAATGCCAGCCAACTTTGTGGCGCCTGAGG
1281












TGAGTGGCCCAGACGCGTGGCGGATGT


PDGFRA
NM_006206
17
4
+
−12
+
0
37
1
0
CGCGAATGCCTCTTTTCTGCAGACTCAGAAGTC
1282












AAAAACCTCCTTACGCGTGGCGGATGT


PDGFRA
NM_006206
17
4
+
23
−
0
37
1
0
CGCGAATGCCTCCAATAAAGTAAGGCCTTCTGA
1283












GTTATCATCTGAACGCGTGGCGGATGT


PDGFRA
NM_006206
17
4
+
58
+
0
37
1
0
CGCGAATGCCTTTGTTGAGCTTCACCTATCAAGT
1284












TGCCCGAGGAAACGCGTGGCGGATGT


PDGFRA
NM_006206
17
4
+
93
−
0
37
1
0
CGCGAATGCCCCTTGAACTTACATTTTTTGAAGC
1285












CAAAAACTCCAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
0
+
0
37
1
0
CGCGAATGCCAACCTAAAAATAAAATATGTGTT
1286












TATGACAAGTTAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
35
−
0
37
1
0
CGCGAATGCCTCTCCAGTTTCTTCATCAAGATGG
1287












GTTTTGATGTGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
70
+
0
37
1
0
CGCGAATGCCAAAGACATCTATCACACTTGATG
1288












TTGGGCCTGAGTACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
105
−
0
37
1
0
CGCGAATGCCAGGTAATCCTCCTGGGCCATCTC
1289












CAGGGTTAAAGGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
140
+
0
37
1
0
CGCGAATGCCATACAAAGAACAGATGACACCCA
1290












AGAACATTTTCCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
175
−
0
37
1
0
CGCGAATGCCGCTTTTGCTCACCACTAGGGTCA
1291












CTGACCCTGTGGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
210
+
0
37
1
0
CGCGAATGCCAGAAGCTGCCAAGCAGAAGAAA
1292












GAAGCAGCAGAAGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
245
−
0
37
1
0
CGCGAATGCCAAGACACAGTCTCTCTCCTGTGA
1293












AATAAATGTCCTACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
280
+
0
37
1
0
CGCGAATGCCTGGCACTGATTCACTCAGATTGT
1294












CTGGGAAAAGACACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
315
−
0
37
1
0
CGCGAATGCCAGGATTTTTGCTACTGATTTCTTC
1295












CTGTTCCTTTAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
350
+
0
37
1
0
CGCGAATGCCGCTAGATCACCAGTAACTGAAAT
1296












AAGAACTCACCTACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
385
−
0
37
1
0
CGCGAATGCCCTGGAGAATCTGGAAGTTCAGAT
1297












TTAAGACTTAAAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
420
+
0
37
1
0
CGCGAATGCCAACCAGTTACAGAAATTAATGAA
1298












GACAGTGTATTAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
455
−
0
37
1
0
CGCGAATGCCTCAACACCTTTTTCTGGTTGGGCA
1299












GTTGGTGGAATACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
490
+
0
37
1
0
CGCGAATGCCTACATTCCTAAGAAGACCTAATT
1300












TCACCAGGGCGAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
525
−
0
37
1
0
CGCGAATGCCACCGCTATCTGATAGAGTCTGTA
1301












AAGGAACTGTAGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
559
+
0
37
1
0
CGCGAATGCCTAGTAGTCAGCACCTTGAACACA
1302












TTCCTCCTAAAGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
595
−
0
37
1
0
CGCGAATGCCTGTTTTTTAGGTCGTGAGTAGTAA
1303












GTTCACTGCTAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
630
+
0
37
1
0
CGCGAATGCCTTAGATTTACTTCACCTGTAAGTT
1304












TGGAGGCACAAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
665
−
0
37
1
0
CGCGAATGCCAGGAGGTTATCTGTAGAGACAGT
1305












CATTTTTTTGCCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
700
+
0
37
1
0
CGCGAATGCCTGTAAATAAAGCTATAAGTAAAA
1306












GTGGCCAACTGCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
735
−
0
37
1
0
CGCGAATGCCACATGAAATATTTGCCTCTAAAT
1307












TAGAACTTGTGGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
770
+
0
37
1
0
CGCGAATGCCTCTCTAAATGAACTCACCTACAA
1308












TAACTTACCAGCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
805
−
0
37
1
0
CGCGAATGCCTTTGATTTTGTTCTTTTAAGTTTTG
1309












GTTTTCATTTACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
840
+
0
37
1
0
CGCGAATGCCCAGAGAAATCTTTAAAATCTCCC
1310












AGTGACACTCTTACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
875
−
0
37
1
0
CGCGAATGCCATCTCACTTTCCTGAAGATTTTCA
1311












TTCCTGCCATCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
910
+
0
37
1
0
CGCGAATGCCTCTAAGTCAACCTAAGAGTCTTA
1312












GCCTGGAAGCAAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
945
−
0
37
1
0
CGCGAATGCCTGTGCAAGAATGTTTTTCTGCAG
1313












AAAGAGGAGAGGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
980
+
0
37
1
0
CGCGAATGCCGTGCCTGAAGGCCTTCTGTTTCCT
1314












GCAGAATATTAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1015
−
0
37
1
0
CGCGAATGCCTCTGGCAATTGGACATGCTTCGT
1315












GTTGTTCTAACAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1050
+
0
37
1
0
CGCGAATGCCGGAAAGTAGCCGTGGAGGCTGTC
1316












ATTCAGAGTCATACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1085
−
0
37
1
0
CGCGAATGCCTTTTTATTTTTAAACCCTTTTTTCT
1317












TGACATCCAAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1120
+
0
37
1
0
CGCGAATGCCTAAGGATGCAAGTAAAAATTTAA
1318












ACCTTTCCAATGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1155
−
0
37
1
0
CGCGAATGCCGCCAGACATCCTAATTTCACTTTG
1319












GTCAGTTTCCTACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1190
+
0
37
1
0
CGCGAATGCCACATGCACAGGACAACCAAGTTC
1320












AAGAACCTCTCAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1225
−
0
37
1
0
CGCGAATGCCCGGGAGAGCTGACTTTAGTTAAT
1321












GAGAGAAGTTTCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1260
+
0
37
1
0
CGCGAATGCCCTGGGCCCACTGAAGATAATGAC
1322












TTGTCTAGGAAGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1295
−
0
37
1
0
CGCGAATGCCCCTGTGTATCTTCTACCAGGTGCT
1323












TGGGCAACTGCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1330
+
0
37
1
0
CGCGAATGCCAAAAAGAAAATCAGCCTGCACCC
1324












CAGCATCAGATCACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1365
−
0
37
1
0
CGCGAATGCCCGACAGGCTAGAAGTTGGCAAAA
1325












GTGGTTCACAATACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1400
+
0
37
1
0
CGCGAATGCCATTGTTAACAGGTCCAAGGAAGA
1326












AGTCACCTCACAACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1435
−
0
37
1
0
CGCGAATGCCTCACTTGAATAAATAATTTTTCGT
1327












GCTGATATTTGACGCGTGGCGGATGT


PALB2
NM_024675
10
16
−
1466
+
11
37
1
0
CGCGAATGCCGTGAAAGGTAAATCAAGATGTGT
1328












TTGATGATGATGACGCGTGGCGGATGT


RB1
NM_000321
8
13
+
0
+
0
37
1
0
CGCGAATGCCAAACAGCTGTTATACCCATTAAT
1329












GGTTCACCTCGAACGCGTGGCGGATGT


RB1
NM_000321
8
13
+
35
−
0
37
1
0
CGCGAATGCCATCCGTGCACTCCTGTTCTGACCT
1330












CGCCTGGGTGTACGCGTGGCGGATGT


RB1
NM_000321
8
13
+
70
+
0
37
1
0
CGCGAATGCCAGCAAAACAACTAGAAAATGAT
1331












ACAAGAATTATTGACGCGTGGCGGATGT


RB1
NM_000321
8
13
+
105
−
0
37
1
0
CGCGAATGCCATCTATATTACATTCATGTTCTTT
1332












ACAGAGAACTTACGCGTGGCGGATGT


RB1
NM_000321
8
13
+
140
+
0
37
1
0
CGCGAATGCCGAGGTAATTTAACTTCATGATTTC
1333












TTTAAAACAGTACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
0
+
0
37
1
0
CGCGAATGCCCAACTCCATGGGAAGAACCTGGT
1334












TTTTAGTGACGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
35
−
0
37
1
0
CGCGAATGCCAGGAGCCCACACCAATTGTCTCC
1335












TTTACCACGTAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
70
+
0
37
1
0
CGCGAATGCCACTCTGAGTGCAAGCGCTGTGTC
1336












CACAAGGCCACCACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
105
−
0
37
1
0
CGCGAATGCCGTCAGGAGGCCCACCTTGACAGC
1337












ATACTCCATGTTACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
140
+
0
37
1
0
CGCGAATGCCCACGTCTCGGCCAAGGCTGCTGG
1338












GTTGGGGGCAGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
175
−
0
37
1
0
CGCGAATGCCGAGCTCAGGCACCATCCCTCCCC
1339












ACCAGACGGGGAACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
210
+
0
37
1
0
CGCGAATGCCTGCAGATGTATGAAAGGTGTGTG
1340












GCCGAGACCTCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
245
−
0
37
1
0
CGCGAATGCCGGGTCCAGGGTCCTTTCTGGCCA
1341












TGGAGCAGGCCAACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
280
+
0
37
1
0
CGCGAATGCCTGTCACCCTGACACTGCCACATG
1342












CACCCCCTTTCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
315
−
0
37
1
0
CGCGAATGCCCTGAAGGATCCCGCTTGCTCTTAT
1343












CAATGACCTGAACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
350
+
0
37
1
0
CGCGAATGCCAAGAGATTGAGATTCTTCTGCGG
1344












TATGGCCAGCACACGCGTGGCGGATGT


RPS6KA1
NM_002953
16
1
+
385
−
0
37
1
0
CGCGAATGCCCCCCCACTCACATCTTTCAGAGT
1345












GATGATGTTGGGACGCGTGGCGGATGT


EPHB1
NM_004441
1
3
+
−41
+
0
37
1
0
CGCGAATGCCCTGCCTCGGCTTGGTCTCGGCCTG
1346












CGGGCCGTCGGACGCGTGGCGGATGT


EPHB1
NM_004441
1
3
+
−6
−
0
37
1
0
CGCGAATGCCAGGAGCAGTAGTAGATAATCCAG
1347












GGCCATCGCCGGACGCGTGGCGGATGT


EPHB1
NM_004441
1
3
+
29
+
0
37
1
0
CGCGAATGCCCCTGGCATCCGCAGTGGCTGCGA
1348












TGGAAGGTAACGACGCGTGGCGGATGT


EPHB1
NM_004441
1
3
+
64
−
0
37
1
0
CGCGAATGCCCCGGCACCAGCAGCCAACTTGCT
1349












CCGTGGAGGGTAACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
0
+
0
37
1
0
CGCGAATGCCGTTCCTGGAAGGTGACGTGAAAG
1350












ATCACTGTGCAGACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
35
−
0
37
1
0
CGCGAATGCCCCAAATGGCAATTGTTCCAGAAG
1351












TCAAGATTGCTGACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
70
+
0
37
1
0
CGCGAATGCCGACTTACTTCTCGGTCAGTGTACT
1352












GCCCTCCTCCCACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
105
−
0
37
1
0
CGCGAATGCCATTTCACAAAAGACCAATGTTGG
1353












TCAGAGACAGGTACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
140
+
0
37
1
0
CGCGAATGCCGGTCGGGTACAGACTCTCATTTG
1354












CTGGCTGGACAAACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
175
−
0
37
1
0
CGCGAATGCCTATGAATAGTGGTATACAAATAT
1355












ATTTCCATCTTTACGCGTGGCGGATGT


PALB2
NM_024675
1
16
−
210
+
0
37
1
0
CGCGAATGCCAGTTAGGGTAAAGTGAAAACACA
1356












ATTTTCTGGATAACGCGTGGCGGATGT


CENTG1
NM_014770
6
12
−
−6
+
0
37
1
0
CGCGAATGCCCCTAAGACTGTACCCCATCTGGA
1357












GACCTGAGCCCCACGCGTGGCGGATGT


CENTG1
NM_014770
6
12
−
29
−
0
37
1
0
CGCGAATGCCTTCACCATGGGAGAAGGAGGGG
1358












GTTCCCGACTCAGACGCGTGGCGGATGT


CENTG1
NM_014770
6
12
−
64
+
0
37
1
0
CGCGAATGCCGAAGCAGAGGAGGAAAAAATTG
1359












ACAACACCATCCAACGCGTGGCGGATGT


CENTG1
NM_014770
6
12
−
99
−
0
37
1
0
CGCGAATGCCCTCACCTTCAGCCTGCCCAGCCG
1360












AGCCTTCAGTCTACGCGTGGCGGATGT


EPHA7
NM_004440
11
6
−
0
+
0
37
1
0
CGCGAATGCCGATCAAAGGGAACGGACCTACTC
1361












AACAGTAAAAACACGCGTGGCGGATGT


EPHA7
NM_004440
11
6
−
35
−
0
37
1
0
CGCGAATGCCGTTTCAGATTATTAATGGAGGCT
1362












GAAGTAGACTTGACGCGTGGCGGATGT


EPHA7
NM_004440
11
6
−
70
+
0
37
1
0
CGCGAATGCCCAGGAACAGTGTATGTTTTCCAG
1363












ATTCGGGCTTTTACGCGTGGCGGATGT


EPHA7
NM_004440
11
6
−
105
−
0
37
1
0
CGCGAATGCCAGTCTGGGACTGTAATTTCCATA
1364












ACCAGCAGCAGTACGCGTGGCGGATGT


EPHA7
NM_004440
11
6
−
140
+
0
37
1
0
CGCGAATGCCTGATGTTGCTACACTAGAGGAAG
1365












CTACAGGTAAAAACGCGTGGCGGATGT


EPHA7
NM_004440
11
6
−
175
−
0
37
1
0
CGCGAATGCCGGAATCCAAACCAAAGGCATAAT
1366












TACCTTCAAACAACGCGTGGCGGATGT


RB1
NM_000321
5
13
+
−50
+
0
37
1
0
CGCGAATGCCCTTCTAAATTACGAAAAAATGTT
1367












AAAAAGTCATAAACGCGTGGCGGATGT


RB1
NM_000321
5
13
+
−15
−
0
37
1
0
CGCGAATGCCAATATATAAGTTCACATGTCCTG
1368












AAAAGAAAAACAACGCGTGGCGGATGT


RB1
NM_000321
5
13
+
20
+
0
37
1
0
CGCGAATGCCTGACACAACCCAGCAGTTCGTAA
1369












GTAGTTCACAGAACGCGTGGCGGATGT


RB1
NM_000321
5
13
+
55
−
0
37
1
0
CGCGAATGCCCATAAAAATCTTTTTTTTTAAGTG
1370












AAAAATAACATACGCGTGGCGGATGT


EPHA4
NM_004438
4
2
−
0
+
0
37
1
0
CGCGAATGCCGTGATTAAAGCCATTGAGGAAGG
1371












CTATCGGTTACCACGCGTGGCGGATGT


EPHA4
NM_004438
4
2
−
35
−
0
37
1
0
CGCGAATGCCGCTGGTGGAGCGCAATGGGGCAG
1372












TCCATTGGAGGGACGCGTGGCGGATGT


EPHA4
NM_004438
4
2
−
70
+
0
37
1
0
CGCGAATGCCTGATGCTAGACTGCTGGCAGAAG
1373












GAGAGGAGCGACACGCGTGGCGGATGT


EPHA4
NM_004438
4
2
−
105
−
0
37
1
0
CGCGAATGCCTCCAACATGTTGACAATCTGCCC
1374












AAATTTAGGCCTACGCGTGGCGGATGT


EPHA4
NM_004438
4
2
−
140
+
0
37
1
0
CGCGAATGCCCAAACTCATCCGCAACCCCAACA
1375












GCTTGAAGAGGAACGCGTGGCGGATGT


EPHA4
NM_004438
4
2
−
175
−
0
37
1
0
CGCGAATGCCAGTAAGCATGGCTGACCTGGAGC
1376












TCTCCGTCCCTGACGCGTGGCGGATGT


EPHA7
NM_004440
1
6
−
−12
+
0
37
1
0
CGCGAATGCCTGTCTTTTTCAGGGATGTGATGA
1377












GTTTAGGGATCAACGCGTGGCGGATGT


EPHA7
NM_004440
1
6
−
23
−
0
37
1
0
CGCGAATGCCGCTGCTCATGATTTTCTTTTGATG
1378












ACCAACCAGTGACGCGTGGCGGATGT


EPHA7
NM_004440
1
6
−
58
+
0
37
1
0
CGCGAATGCCATTCAGACTATGAGAGCACAAAT
1379












GCTACATTTACAACGCGTGGCGGATGT


EPHA7
NM_004440
1
6
−
93
−
0
37
1
0
CGCGAATGCCGGAGAAATGCATATCACACTTGA
1380












ATGCCAGTTCCAACGCGTGGCGGATGT


RPS6KA1
NM_002953
11
1
+
−26
+
0
37
1
0
CGCGAATGCCTTGATGAGTCCCGGGGGCTGTTT
1381












CAGGGCGAAGCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
11
1
+
9
−
0
37
1
0
CGCGAATGCCTCTGGGCTTCAGTGCTCAGAAAC
1382












TGGGGCATGCCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
11
1
+
44
+
0
37
1
0
CGCGAATGCCGCCTCTTGCGGGCCCTGTTCAAG
1383












CGGAATCCTGCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
11
1
+
79
−
0
37
1
0
CGCGAATGCCCCTCCCCTGAGCTGGGGCTGCTT
1384












ACCGAGCCGGTTACGCGTGGCGGATGT


EPHB1
NM_004441
2
3
+
−38
+
0
37
1
0
CGCGAATGCCTTGTTTTTGTTTATTCGTTTTTTCT
1385












TTTTAATCTAACGCGTGGCGGATGT


EPHB1
NM_004441
2
3
+
−3
−
0
37
1
0
CGCGAATGCCTGCAGTAGCCGTTCTGGTGTCCA
1386












TTAACGTTTCTGACGCGTGGCGGATGT


EPHB1
NM_004441
2
3
+
32
+
0
37
1
0
CGCGAATGCCGAGCTGGGCTGGACGGCCAATCC
1387












TGCGTCCGGGGTACGCGTGGCGGATGT


EPHB1
NM_004441
2
3
+
67
−
0
37
1
0
CGCGAATGCCCATAGCAGGACTGAAAGACGAAT
1388












GGTTTGATACTCACGCGTGGCGGATGT


PDGFRA
NM_006206
19
4
+
−14
+
0
37
1
0
CGCGAATGCCCTCCTTCCTTGCAGACCTTTCTGC
1389












CCGTGAAGTGGACGCGTGGCGGATGT


PDGFRA
NM_006206
19
4
+
21
−
0
37
1
0
CGCGAATGCCGTGTAGAGGTTGTCAAAGATGCT
1390












CTCAGGAGCCATACGCGTGGCGGATGT


PDGFRA
NM_006206
19
4
+
56
+
0
37
1
0
CGCGAATGCCCACACTGAGTGATGTCTGGTCTT
1391












ATGGCATTCTGCACGCGTGGCGGATGT


PDGFRA
NM_006206
19
4
+
91
−
0
37
1
0
CGCGAATGCCTGTCAGGCCCATACCAAGGGAAA
1392












AGATCTCCCAGAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
0
+
0
37
1
0
CGCGAATGCCAGGAAAACTTTGAGTTCCTGATC
1393












GTGTCCAGCACGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
35
−
0
37
1
0
CGCGAATGCCTCAAAACTGGCTGCCTCAAAGTG
1394












CCACGTCTGACCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
70
+
0
37
1
0
CGCGAATGCCGGAGCGGGATGCCTGGGTCCAGG
1395












CCATCGAGAGTCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
105
−
0
37
1
0
CGCGAATGCCGCTGCTCTCACAGCATTGCAGAC
1396












TGGCTAGGATCTACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
140
+
0
37
1
0
CGCGAATGCCAAGGTCAAGGTAAGAGTTTGAGG
1397












TGGAGTGGAGGAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
175
−
0
37
1
0
CGCGAATGCCTGGCCGTCAGCACCAGGGTCAGT
1398












TCCTGCGGCCAGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
210
+
0
37
1
0
CGCGAATGCCTTGGCAACGACACGGCCGAAGTC
1399












GTGTGAGAGGAGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
245
−
0
37
1
0
CGCGAATGCCCTCCAAGCCCCACCTCCTTCTCAG
1400












ACACCTCTGCTACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
280
+
0
37
1
0
CGCGAATGCCGCAGAGTCAGTGCCAACCCAAAC
1401












CCTCTCTGCAGCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
315
−
0
37
1
0
CGCGAATGCCGATGGCCACGGCCTCGCTTTGGC
1402












TGTCTGTGCGCAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
350
+
0
37
1
0
CGCGAATGCCCAGGCGATCCGGAACGCCAAGG
1403












GGAATTCAATCTGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
385
−
0
37
1
0
CGCGAATGCCCCTCCTGGCACTCACTGGGGGCC
1404












CCGCAGTCCACGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
420
+
0
37
1
0
CGCGAATGCCCAGCGGAGGGGCTAGGGAGTGT
1405












AGTGAATGCCGGGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
455
−
0
37
1
0
CGCGAATGCCGTAGAGGTCGGCTCCCAGCCGGG
1406












CAGCACAGGCACACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
490
+
0
37
1
0
CGCGAATGCCGCTCCTTCCGCAGACCCCACGTG
1407












GGCCAGCTTGAAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
525
−
0
37
1
0
CGCGAATGCCTGCCAGAACACTCGATGCAGATG
1408












AGGGCGCCCAGGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
560
+
0
37
1
0
CGCGAATGCCTCCACCGCAACCTGGGCACACAC
1409












CTGTCCCGCGTTACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
595
−
0
37
1
0
CGCGAATGCCAGCTCCCGTGGCCAGTCGTCCAA
1410












GTCCAGCGAGCGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
633
+
0
37
1
0
CGCGAATGCCCCTGGTGCTGACGGCTATTGGCA
1411












ACGACACGGCCAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
665
−
0
37
1
0
CGCGAATGCCACGGCCTCGCGTGTCGCTTTCCC
1412












ACACGCGGTTGGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
700
+
0
37
1
0
CGCGAATGCCGCCAAGCCCTCGCGGGACTCTTC
1413












GCGGTAAGCGTGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
735
−
0
37
1
0
CGCGAATGCCACACCCTCAGCAACCCTCCCCCC
1414












GCTCTGTTCCCTACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
770
+
0
37
1
0
CGCGAATGCCGCGAACCTGAGACGGTCCCGTGG
1415












GTAGGGGCAGAAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
805
−
0
37
1
0
CGCGAATGCCCAACGGAAAAGGCTCTAGGGACC
1416












CCCAGCCAGGACACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
840
+
0
37
1
0
CGCGAATGCCATCCCTGGTCTTGCAGGGAGGAG
1417












CGCGAGTCGTGGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
875
−
0
37
1
0
CGCGAATGCCGCCAGGAACAGTAGCTGCTCGTA
1418












CTTGGCGCGAATACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
910
+
0
37
1
0
CGCGAATGCCGCCGCTGAGCACCTCGGAGGAGC
1419












CGCTGGGCCGCCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
945
−
0
37
1
0
CGCGAATGCCAGCCACGTCCTGGGCCTGCACGG
1420












CGGCCCACAGCTACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
980
+
0
37
1
0
CGCGAATGCCACCGTTCTCCTGCTTTTGGCCCAT
1421












GCGCGACACGGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1015
−
0
37
1
0
CGCGAATGCCGCAGCTGTGGGTCCTCTACGCTG
1422












GTGTCGAGCGGCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1050
+
0
37
1
0
CGCGAATGCCGCTCCCCACTCCACCTGGCGGCC
1423












GAGCTCGCCCACACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1085
−
0
37
1
0
CGCGAATGCCTCACGTACCCACAGCAGCAGTTG
1424












CGTGATGACGACACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1120
+
0
37
1
0
CGCGAATGCCGCTGGGGGGAGGAAAGGGGGTC
1425












TTTGAGGCTTCATACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1155
−
0
37
1
0
CGCGAATGCCAATTTCGGGCTTTCCCGCGCCAG
1426












GCGTTTTCCGAGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1190
+
0
37
1
0
CGCGAATGCCCCCAGAGGGACCCCGGAAGTAG
1427












GCTTGGCCATGTGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1225
−
0
37
1
0
CGCGAATGCCGTCCCCAGGGCGCCCACACCCGG
1428












CGCCGCCTCCCCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1260
+
0
37
1
0
CGCGAATGCCGCGCGGTCACGCGGCCGTTTCCG
1429












CCCTCTAGTACGACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1295
−
0
37
1
0
CGCGAATGCCGCGGCCCTGGGCGTCACGGGCCG
1430












CCACGTCCGCGCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1330
+
0
37
1
0
CGCGAATGCCACGGCGCTGTTCTACGCCCGCCA
1431












GGCTGGAAGCCAACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1365
−
0
37
1
0
CGCGAATGCCGGCAGCCGTGCTGGAGAAGGATG
1432












TCGGCGCACAGCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1400
+
0
37
1
0
CGCGAATGCCCGGGTGAGGGCGGCAGCGCGGC
1433












CACCACGCCCAGCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1435
−
0
37
1
0
CGCGAATGCCCTGGGCGTGGCGGTGATGCTGGG
1434












CGTGGTGGCCGCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1470
+
0
37
1
0
CGCGAATGCCCCCCCGCCGCCGGAGCAGCGCCG
1435












CTAGCGTGGGCCACGCGTGGCGGATGT


CENTG1
NM_014770
5
12
−
1505
−
0
37
1
0
CGCGAATGCCGGGCAACTATACCAGCGCAACCG
1436












GGGCGTCGGCGCACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
0
+
0
37
1
0
CGCGAATGCCGGCCTCCATCTGCACCACAGAAC
1437












CTCATTTTCAACACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
35
−
0
37
1
0
CGCGAATGCCGGACTCCATTCCAAACTTACTGT
1438












GGTTTGGTTGATACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
70
+
0
37
1
0
CGCGAATGCCTCCTGCAGACAATGGGGGAAGAA
1439












ACGATGTGACCTACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
105
−
0
37
1
0
CGCGAATGCCCTGCTCCCAACTGCACCGCTTAC
1440












ACAATATTCTGTACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
140
+
0
37
1
0
CGCGAATGCCGGCGAATGTGTTCCCTGTGGGAG
1441












TAACATTGGATAACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
175
−
0
37
1
0
CGCGAATGCCCATAGTTATCCTCTAATCCAGTCT
1442












GCTGGGGCATGACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
210
+
0
37
1
0
CGCGAATGCCTCACTGTCATGGACCTGCTAGCC
1443












CACGCTAATTATACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
245
−
0
37
1
0
CGCGAATGCCTCAGAAACTCCATTTACAGCTTC
1444












AACTTCAAAAGTACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
280
+
0
37
1
0
CGCGAATGCCCTTAAGCCGATCCCAGAGGCTCT
1445












TTGCTGCTGTCAACGCGTGGCGGATGT


EPHA7
NM_004440
13
6
−
315
−
0
37
1
0
CGCGAATGCCAACACAAAACATACCTGCTTGAC
1446












CAGTGGTGATACACGCGTGGCGGATGT


CHAF1A
NM_005483
7
19
+
−36
+
0
37
1
0
CGCGAATGCCTCTCCTCTTTCTCATCACCATCTC
1447












TTAACATCACAACGCGTGGCGGATGT


CHAF1A
NM_005483
7
19
+
−1
−
0
37
1
0
CGCGAATGCCACCTCGTGATTTCGGCCTTCTCTG
1448












CTTTAATGCGCACGCGTGGCGGATGT


CHAF1A
NM_005483
7
19
+
34
+
0
37
1
0
CGCGAATGCCTCTTCCAGAAACCAAAGACTCCA
1449












CAGGCCCCCAAGACGCGTGGCGGATGT


CHAF1A
NM_005483
7
19
+
69
−
0
37
1
0
CGCGAATGCCCTGAAACCCAAAAGCAAAGGCA
1450












GCCGGCTGCTCACACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
0
+
0
37
1
0
CGCGAATGCCGCCCAGGAGAAATTAACAGAATC
1451












CAACCAGAAGCTACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
35
−
0
37
1
0
CGCGAATGCCCAAGTCTCCGCTCCAGAGCCTCC
1452












CGCAGCAGCCCCACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
70
+
0
37
1
0
CGCGAATGCCGGGAGCTGCCCGCCGACCACCCC
1453












AAGGGGCGGCTGACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
105
−
0
37
1
0
CGCGAATGCCGCAGCGGAGGAGGCCGCAGCGA
1454












GCTCTTCTCGCAGACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
140
+
0
37
1
0
CGCGAATGCCCTTCAGCACCCGCCTGGCCGGGC
1455












CCTTTCCCGCCAACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
175
−
0
37
1
0
CGCGAATGCCGAGCGGCGCGGGCTTGCACAGGG
1456












TGCTGTAGTGCGACGCGTGGCGGATGT


PKN1
NM_002741
6
19
+
210
+
0
37
1
0
CGCGAATGCCACAGGTGGGTCTGAGACCCTACC
1457












CCACCCCTGCAGACGCGTGGCGGATGT


EPHA3
NM_005233
2
3
+
−38
+
0
37
1
0
CGCGAATGCCTGTTATTAACTGTGTTTGTGTATT
1458












ATGTTTTATTTACGCGTGGCGGATGT


EPHA3
NM_005233
2
3
+
−3
−
0
37
1
0
CGCGAATGCCCCCTTGAATTGTTTTTGAATCCAG
1459












TAGATTGACTAACGCGTGGCGGATGT


EPHA3
NM_005233
2
3
+
32
+
0
37
1
0
CGCGAATGCCGAGCTGGGCTGGATCTCTTATCC
1460












ATCACATGGGGTACGCGTGGCGGATGT


EPHA3
NM_005233
2
3
+
67
−
0
37
1
0
CGCGAATGCCGAGAAAATGTTTCCTTGTGATAG
1461












TTTATTGAACTCACGCGTGGCGGATGT


KSR2
NM_173598
5
12
−
−3
+
0
37
1
0
CGCGAATGCCCAGGGCATGGGCTACCTCCACGC
1462












CAAGGGAATCCTACGCGTGGCGGATGT


KSR2
NM_173598
5
12
−
32
−
0
37
1
0
CGCGAATGCCCATAGAAGACGTTCTTTGACTTG
1463












AGGTCCTTGTGTACGCGTGGCGGATGT


KSR2
NM_173598
5
12
−
67
+
0
37
1
0
CGCGAATGCCACAACGGCAAAGTGGTCATCACG
1464












GACTTTGGACTCACGCGTGGCGGATGT


KSR2
NM_173598
5
12
−
102
−
0
37
1
0
CGCGAATGCCCACCTGCCAGCCTGCAGCACCCC
1465












AGAAATGCTGAAACGCGTGGCGGATGT


RPS6KA1
NM_002953
1
1
+
−38
+
0
37
1
0
CGCGAATGCCGGCCGCCGGAGGAGCGCGGGTG
1466












ACCTGGCGGCGGCACGCGTGGCGGATGT


RPS6KA1
NM_002953
1
1
+
−3
−
0
37
1
0
CGCGAATGCCGGCCAGGGCTCCTTGAGCTGGGC
1467












GAGCGGCATCTCACGCGTGGCGGATGT


RPS6KA1
NM_002953
1
1
+
32
+
0
37
1
0
CGCGAATGCCGCTCATGGAGCTAGTGCCTCTGG
1468












ACCCGGAGGTGAACGCGTGGCGGATGT


RPS6KA1
NM_002953
1
1
+
63
−
27
37
1
0
CGCGAATGCCGCCGCGGGCGCCCGTCCCCCGCC
1469












CCGCTCACTCACACGCGTGGCGGATGT


KSR2
NM_173598
18
12
−
0
+
0
37
1
0
CGCGAATGCCGAAATCTCCCCCGGCCAGCTGAG
1470












CCTGGAGGACCTACGCGTGGCGGATGT


KSR2
NM_173598
18
12
−
35
−
0
37
1
0
CGCGAATGCCCAGTCTCGCACACCTGTTCATCC
1471












GTCATCTCCAAGACGCGTGGCGGATGT


KSR2
NM_173598
18
12
−
70
+
0
37
1
0
CGCGAATGCCTGGAGAAATACGGAGCCAACCG
1472












GGAGGAGTGTGCCACGCGTGGCGGATGT


KSR2
NM_173598
18
12
−
105
−
0
37
1
0
CGCGAATGCCACATTCCTGAGGCAGGAGAGGGA
1473












GGCGTTGAGGCGACGCGTGGCGGATGT


KSR2
NM_173598
18
12
−
140
+
0
37
1
0
CGCGAATGCCCCACATGTCAGGTGAGCAGGCCC
1474












CGGGGTCGGGGAACGCGTGGCGGATGT


GUCY2F
NM_001522
10
X
−
0
+
0
37
1
0
CGCGAATGCCAGCTGCTGTGGACGGCCCCTGAA
1475












CTGTTGAGAGCTACGCGTGGCGGATGT


GUCY2F
NM_001522
10
X
−
35
−
0
37
1
0
CGCGAATGCCTCTCCTGCAAAAGAACCTAACCT
1476












GCTGCCTCTTGGACGCGTGGCGGATGT


GUCY2F
NM_001522
10
X
−
70
+
0
37
1
0
CGCGAATGCCTGTCTATAGCTTTGCCATCATCAT
1477












GCAAGAAGTGAACGCGTGGCGGATGT


GUCY2F
NM_001522
10
X
−
105
−
0
37
1
0
CGCGAATGCCCAGATCCATCATGCAGAATGGGG
1478












TACCCCGGACCAACGCGTGGCGGATGT


GUCY2F
NM_001522
10
X
−
140
+
0
37
1
0
CGCGAATGCCCCAGCTCAAGGTAAGCGGGAGGT
1479












GAGAAAAGGGCCACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
0
+
0
37
1
0
CGCGAATGCCAGTGAGCCTCGCAGCTGGTCCCT
1480












GCTAGAGCAGCTACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
35
−
0
37
1
0
CGCGAATGCCCCCCGGGGGCCGCCAGGTCTGCC
1481












CCGGCCAGGCCCACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
70
+
0
37
1
0
CGCGAATGCCTACAGCAGCAGCTGGAGCTGGAG
1482












CGGGAGCGGCTGACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
105
−
0
37
1
0
CGCGAATGCCTCCTTCAGCTTCAGCTCCTTGCGG
1483












ATTTCCCGCCGACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
140
+
0
37
1
0
CGCGAATGCCGGGTGCTGAGAACCTGCGGCGGG
1484












CCACCACTGACCACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
175
−
0
37
1
0
CGCGAATGCCCAGCAGCAGCTCTACGGGGCCCA
1485












GGCTGCGGCCCAACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
210
+
0
37
1
0
CGCGAATGCCCGGGGCTCCTCGCGCCGCCTCGA
1486












CCTGCTGCACCAACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
245
−
0
37
1
0
CGCGAATGCCGAAGCACCACGTGGGCGTGCAGC
1487












TCCTGCAGCTGCACGCGTGGCGGATGT


PKN1
NM_002741
2
19
+
280
+
0
37
1
0
CGCGAATGCCCCGACCCGGCGGCCACCCACGGT
1488












GAGCTGGGATGCACGCGTGGCGGATGT


RBBP8
NM_002894
7
18
+
0
+
0
37
1
0
CGCGAATGCCGAATGATCAACAGCATCAAGCAG
1489












CTGAGCTTGAATACGCGTGGCGGATGT


RBBP8
NM_002894
7
18
+
35
−
0
37
1
0
CGCGAATGCCTGTTATCGGTGAATCTGGAATAA
1490












CGTCTTCCTCACACGCGTGGCGGATGT


RBBP8
NM_002894
7
18
+
70
+
0
37
1
0
CGCGAATGCCGCCTTCTCATTTTCTGGCGTTAAC
1491












CGGCTACGAAGACGCGTGGCGGATGT


RBBP8
NM_002894
7
18
+
105
−
0
37
1
0
CGCGAATGCCTTTGTTCTATGTATCGGACATGGG
1492












GGTTCTCCTTTACGCGTGGCGGATGT


RBBP8
NM_002894
7
18
+
140
+
0
37
1
0
CGCGAATGCCCACATACTAAATTGGAGCACTCT
1493












GTGTGTGCAAATACGCGTGGCGGATGT


RBBP8
NM_002894
7
18
+
175
−
0
37
1
0
CGCGAATGCCAACCAGAGAACTAAAATACAACT
1494












CCAACTCTTACCACGCGTGGCGGATGT


EPHB1
NM_004441
13
3
+
0
+
0
37
1
0
CGCGAATGCCGGAGGGAAGATCCCTGTGAGATG
1495












GACAGCTCCAGAACGCGTGGCGGATGT


EPHB1
NM_004441
13
3
+
35
−
0
37
1
0
CGCGAATGCCCGCTGGCTGAAGTGAACTTGCGG
1496












TAGGCGATGGCCACGCGTGGCGGATGT


EPHB1
NM_004441
13
3
+
70
+
0
37
1
0
CGCGAATGCCACGTTTGGAGCTATGGGATCGTC
1497












ATGTGGGAAGTCACGCGTGGCGGATGT


EPHB1
NM_004441
13
3
+
105
−
0
37
1
0
CGCGAATGCCGACATATCCCAATAGGGTCTCTC
1498












TCCAAATGACATACGCGTGGCGGATGT


EPHB1
NM_004441
13
3
+
140
+
0
37
1
0
CGCGAATGCCCAACCAAGATGTGAGTGTCAGCA
1499












GCACTTGGTCACACGCGTGGCGGATGT


PDGFRA
NM_006206
12
4
+
−4
+
0
37
1
0
CGCGAATGCCATAGAAACCGAGGTATGAAATTC
1500












GCTGGAGGGTCAACGCGTGGCGGATGT


PDGFRA
NM_006206
12
4
+
31
−
0
37
1
0
CGCGAATGCCAATATATTCATGTCCATCTGGGCT
1501












GATTGATTCAAACGCGTGGCGGATGT


PDGFRA
NM_006206
12
4
+
66
+
0
37
1
0
CGCGAATGCCTATGTGGACCCGATGCAGCTGCC
1502












TTATGACTCAAGACGCGTGGCGGATGT


PDGFRA
NM_006206
12
4
+
101
−
0
37
1
0
CGCGAATGCCTACCAAGCACTAGTCCATCTCTT
1503












GGAAACTCCCATACGCGTGGCGGATGT


RET
NM_020630
7
10
+
0
+
0
37
1
0
CGCGAATGCCATCGGGAAAGTCTGTGTGGAAAA
1504












CTGCCAGGCATTACGCGTGGCGGATGT


RET
NM_020630
7
10
+
35
−
0
37
1
0
CGCGAATGCCAGGAATGCAGCTTGTACTGGACG
1505












TTGATGCCACTGACGCGTGGCGGATGT


RET
NM_020630
7
10
+
70
+
0
37
1
0
CGCGAATGCCCTGGTGCCAACTGCAGCACGCTA
1506












GGGGTGGTCACCACGCGTGGCGGATGT


RET
NM_020630
7
10
+
105
−
0
37
1
0
CGCGAATGCCTTCACAAACAGGATCCCCGAGGT
1507












GTCCTCGGCTGAACGCGTGGCGGATGT


RET
NM_020630
7
10
+
140
+
0
37
1
0
CGCGAATGCCTGACACCAAGGCCCTGCGGCGGC
1508












CCAAGTGTGCCGACGCGTGGCGGATGT


RET
NM_020630
7
10
+
175
−
0
37
1
0
CGCGAATGCCCTGCTGGTCGGTGGCCACCACCA
1509












TGTAGTGAAGTTACGCGTGGCGGATGT


RET
NM_020630
7
10
+
210
+
0
37
1
0
CGCGAATGCCACCTCTAGGCAGGCCCAGGCCCA
1510












GCTGCTTGTAACACGCGTGGCGGATGT


RET
NM_020630
7
10
+
245
−
0
37
1
0
CGCGAATGCCCTCCCTGGAGCAGGCACTCACAT
1511












GACCCCTCCACTACGCGTGGCGGATGT


EPHA7
NM_004440
3
6
−
0
+
0
37
1
0
CGCGAATGCCGTTATAAAAGCAATAGAAGAAG
1512












GTTATCGTTTACCACGCGTGGCGGATGT


EPHA7
NM_004440
3
6
−
35
−
0
37
1
0
CGCGAATGCCGCTGGTGAAGGCCAGCTGGGCAG
1513












TCCATGGGTGCTACGCGTGGCGGATGT


EPHA7
NM_004440
3
6
−
70
+
0
37
1
0
CGCGAATGCCTAATGTTGGATTGTTGGCAAAAG
1514












GAGCGTGCTGAAACGCGTGGCGGATGT


EPHA7
NM_004440
3
6
−
105
−
0
37
1
0
CGCGAATGCCTCTAGAATTCCAACTATCTGTTCA
1515












AATTTTGGCCTACGCGTGGCGGATGT


EPHA7
NM_004440
3
6
−
140
+
0
37
1
0
CGCGAATGCCCAAAATGATTCGAAACCCAAATA
1516












GTCTGAAAACTCACGCGTGGCGGATGT


EPHA7
NM_004440
3
6
−
175
−
0
37
1
0
CGCGAATGCCCTTAGGCATTTCTTACCTACTACA
1517












AGTTCCCAGGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
0
+
0
37
1
0
CGCGAATGCCATGGCGAGTTGCTCCTTCACTCG
1518












CGACCAAGCGACACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
35
−
0
37
1
0
CGCGAATGCCCCGCTGCCGCCGCTGCTGCACCT
1519












CTTAGTCTTCTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
70
+
0
37
1
0
CGCGAATGCCCAGCTCTAGCAGCAGTGGTGACC
1520












ACCCCGCTTCTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
105
−
0
37
1
0
CGCGAATGCCCCGGTCCCAATGAGTGCGGTCGG
1521












GGTTCCCGAGGAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
140
+
0
37
1
0
CGCGAATGCCGTCGTCTTGTCCGGGAGCCATGT
1522












GGCTCTCCACGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
175
−
0
37
1
0
CGCGAATGCCCTCTTCGGACTCGGAGTCTGACC
1523












GGGAGCCAGTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
210
+
0
37
1
0
CGCGAATGCCGAGGACCTGCCCGTCGGGGAGGA
1524












AGTCTGCAAACGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
245
−
0
37
1
0
CGCGAATGCCGCCTGTGCCCATGCTTCTGTTTCC
1525












GCAGGTAGCCGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
280
+
0
37
1
0
CGCGAATGCCGCTACTTCGTGCTCAAACTCGAG
1526












ACTGCTGACGCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
315
−
0
37
1
0
CGCGAATGCCTTCCTGGCATTTTCGTAGTATTCC
1527












AGCCGAGCTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
350
+
0
37
1
0
CGCGAATGCCGTTCCGGCACAGTGTCCGCGCCG
1528












CGGCGGCTGCAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
385
−
0
37
1
0
CGCGAATGCCCGGGGGGATCGCGGCGCCAGAG
1529












GCGGCCGCCGCTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
420
+
0
37
1
0
CGCGAATGCCCTCATTCCACCGCGGCGCGTGAT
1530












CACCCTATACCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
455
−
0
37
1
0
CGCGAATGCCACCTTGCATCTGCTCGCTGGCTCA
1531












CGGAAAAGCACACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
490
+
0
37
1
0
CGCGAATGCCACCGACACCTCATTGCTCTTTTCA
1532












CCCAAGACGAAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
525
−
0
37
1
0
CGCGAATGCCTGCTCCGACTCGTTCTCGGCCACC
1533












ATCGCGAAGTAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
560
+
0
37
1
0
CGCGAATGCCGGAAAGCTGGTACTTGCTGCTCA
1534












GCCGCCTCATCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
595
−
0
37
1
0
CGCGAATGCCGCCGAGCGTGCCGCAGCGGCGGC
1535












GCTTGCTCTCGAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
630
+
0
37
1
0
CGCGAATGCCGCGCAGCCGGACGGAGAGCCGG
1536












CCGCGCTGGCGGCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
665
−
0
37
1
0
CGCGAATGCCCATCTTTATAGAAGGGTGGCTCC
1537












GCCGCCGCTGCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
700
+
0
37
1
0
CGCGAATGCCTGTGGCAGGTAATAGTCAAACCC
1538












AGGGGGCTGGGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
735
−
0
37
1
0
CGCGAATGCCCACAGCCGGAACACGCCGCTCAG
1539












CTCTTTTCTGTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
770
+
0
37
1
0
CGCGAATGCCTCTAACCGACGAGGAGGTCGTGT
1540












TTGTGAGGCTGAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
805
+
0
37
1
0
CGCGAATGCCCAGGAGCTGGACGACCACGCTGG
1541












CCACTTCGGTGTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
840
+
0
37
1
0
CGCGAATGCCAGCATCCGTCGCTGTGGACACTC
1542












GGAGCAGTATTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
875
−
0
37
1
0
CGCGAATGCCGACCGATGACAGTGGACCTGCCT
1543












ACTTCCAAGAAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
910
+
0
37
1
0
CGCGAATGCCCGGGAGAGCTCTGGATGCAGGTC
1544












GATGACTGTGTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
945
−
0
37
1
0
CGCGAATGCCTTCTCCAAAAACAGCTCATGCAT
1545












GTTTTGGGCAACACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
980
+
0
37
1
0
CGCGAATGCCGATGAGAGCCTTGTGTGCAGACG
1546












AATACAGAGCCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1015
−
0
37
1
0
CGCGAATGCCGTGGGCGCCGATGCTGATGCTGT
1547












AGCTGCGGCAGCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1050
+
0
37
1
0
CGCGAATGCCCTGTTAACCCTGCTGTCCGCTAG
1548












GAGGCACCTGGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1085
−
0
37
1
0
CGCGAATGCCTTCTGAGCCAGCCTCCCGGCTCG
1549












AGCGGCACCAAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1120
+
0
37
1
0
CGCGAATGCCGGTCCCGCTTTGAGCAGTTTTGCC
1550












ACCTCAGGGCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1155
−
0
37
1
0
CGCGAATGCCCTGGTGAAAAGCATCTCGTCTTC
1551












CCCGTCGCCGATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1190
+
0
37
1
0
CGCGAATGCCGCGCTTCGTAACACCCAGCGAGC
1552












CTGTGGCCCACTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1225
−
0
37
1
0
CGCGAATGCCGCGCCCTCTGGGCAGGTGCAGTC
1553












TTCCTCGCCTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1260
+
0
37
1
0
CGCGAATGCCAGGTCAAGGAGAGCGGTTTCAGT
1554












GCCGGCCAGCTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1295
−
0
37
1
0
CGCGAATGCCGGGGACGTGCTGGGCTGGGTGCT
1555












AAGCGGCGAAAAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1330
+
0
37
1
0
CGCGAATGCCGGCACCCTGCAGAAGCCCCGAAC
1556












AATGGAGCTCGCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1365
−
0
37
1
0
CGCGAATGCCTTGCCAGAGCCAGAACCAGACAC
1557












TTCAGAAGACAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1400
+
0
37
1
0
CGCGAATGCCCTTTGGGGAGGAAGGCAATCCCC
1558












AGGGCAAAGAAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1435
−
0
37
1
0
CGCGAATGCCCATAGGCATGTAGTCACCTCCGC
1559












TTCCTTCCTGATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1470
+
0
37
1
0
CGCGAATGCCAACAATTGGGGCTCAGGAAATGG
1560












CCGGGGCTCAGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1505
−
0
37
1
0
CGCGAATGCCTACTGGAGCCTTGGCCATTTGAG
1561












CCCTGGCCACCTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1540
+
0
37
1
0
CGCGAATGCCGCCATAGCTCGGGAGGAAACCAG
1562












TGTTCAGGCGAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1575
−
0
37
1
0
CGCGAATGCCCCATTTGAGCCCTGACCACCTCG
1563












GGATCCCTGTCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1610
+
0
37
1
0
CGCGAATGCCCCAGGGCTCAGGAGGAAACCAGT
1564












GCTCTAGAGATGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1645
−
0
37
1
0
CGCGAATGCCACCACCTGAACCGTGCCCACCTG
1565












CGGTGCCCTGGCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1680
+
0
37
1
0
CGCGAATGCCGGCCAGAGACCTGGAGGTGGGC
1566












ATGGCTCAGGTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1715
−
0
37
1
0
CGCGAATGCCCACCTGAGCCATGGCCATCTCCA
1567












GGTCCCTGGCCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1750
+
0
37
1
0
CGCGAATGCCGTGGCAAGAACTCTGGGGGGGGC
1568












AAAGGCTCAGGAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1785
−
0
37
1
0
CGCGAATGCCCCACGTTCACCATCACCATCGGA
1569












TCCTTTCCCACTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1820
+
0
37
1
0
CGCGAATGCCAAAATCTCTGAAGAAAAGATCCT
1570












ATTTTGGCAAATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1855
−
0
37
1
0
CGCGAATGCCAGGTGGTGGCATTTGCTGTTGCTT
1571












GCTTTGAGTTAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1890
+
0
37
1
0
CGCGAATGCCCCACCACCTCCTCCTCCACCCCCA
1572












CCAGCTGGAGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1925
−
0
37
1
0
CGCGAATGCCTGAATCTTCCCCCAGACTTCCCTT
1573












TTCCACCAGTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1960
+
0
37
1
0
CGCGAATGCCGACTTTATTTTTGTGTTGACAGAG
1574












GAGCCACGAAAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
1995
−
0
37
1
0
CGCGAATGCCTCTGCATCTTTCACTTCTTTGGCT
1575












TCTTTGCATTCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2030
+
0
37
1
0
CGCGAATGCCGATCCCAGAAGGTGCAGCTCGAG
1576












GTCCCCACAGAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2065
−
0
37
1
0
CGCGAATGCCGTATGGGTCATCCTCATCTTCATC
1577












AAAAGCTCTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2100
+
0
37
1
0
CGCGAATGCCGTGCCAATGAGGCCAGGGGTGGC
1578












CACCCCTCTTGTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2135
−
0
37
1
0
CGCGAATGCCTTTGAGGAGCCATTGGCATATAA
1579












TCACTGGAGCTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2170
+
0
37
1
0
CGCGAATGCCATGTCTCTGCTTCAAAAAAGCGC
1580












CACTCTCGATCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2205
−
0
37
1
0
CGCGAATGCCAACATCATCATGTACCCTCTTGA
1581












ATCTTCAAAAGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2240
+
0
37
1
0
CGCGAATGCCTCCCAGAGTGAGCCCACCACCTG
1582












CTCCGAGTCCTCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2275
−
0
37
1
0
CGCGAATGCCTGAGTCATCCTCTTTATTAGTATC
1583












AGGTGCTTTTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2310
+
0
37
1
0
CGCGAATGCCAAGGACAATGACAGTGAGAGTG
1584












ACTACATGTTTATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2345
−
0
37
1
0
CGCGAATGCCTGGGGTTTTTTGGAATTGCACCG
1585












GCTCCAGGAGCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2380
+
0
37
1
0
CGCGAATGCCGAAATCCTCAGGGTGGCTCTTCC
1586












TCCAAAAGTTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2415
−
0
37
1
0
CGCGAATGCCCTCCGAAAAGGGTTTGGTAGAGA
1587












GAAGTAGGAGCTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2450
+
0
37
1
0
CGCGAATGCCCTCACCTTTGGGACAGAATGACA
1588












ACAGTGAGTATGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2485
−
0
37
1
0
CGCGAATGCCGCCCCTCCCCAGGAACTTTCCAG
1589












GTAACATTGGCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2520
+
0
37
1
0
CGCGAATGCCCTAGACAAAGAAGTCTCCTATAA
1590












CTGGGACCCCAAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2555
−
0
37
1
0
CGCGAATGCCATGATCCCTCACCTGAAGGCTTT
1591












GAAGCTGCATCTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2590
+
0
37
1
0
CGCGAATGCCTCTCAAAGCCTGGAGATGGGGGA
1592












TCACCTTCAAAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2625
−
0
37
1
0
CGCGAATGCCTTAGCTTTATTCTTTGGGGGCTCA
1593












TGATCTGAAGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2660
+
0
37
1
0
CGCGAATGCCGAGACCTAACCGACTTTCTTTTAT
1594












TACAAAAGGATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2695
−
0
37
1
0
CGCGAATGCCATGTGTGGGCTTTTGTGGTTTTGG
1595












CTTGATTTTATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2730
+
0
37
1
0
CGCGAATGCCGAGCAGAGAGAAGCTGACAGCT
1596












CTAGTGACTACGTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2765
−
0
37
1
0
CGCGAATGCCGTGTATTGCTCTCTCTTTTAGTGA
1597












AGTCCATGTTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2800
+
0
37
1
0
CGCGAATGCCCAGCTCCCTCTACTCAAGGACTA
1598












CCAGATTCGTGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2835
−
0
37
1
0
CGCGAATGCCGAAAAGGCTGACTGTCTGGGTTC
1599












AGCAATTATGCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2870
+
0
37
1
0
CGCGAATGCCTAATTATGTGAATGTTGAGTTTG
1600












GAGTGCCATTTCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2905
−
0
37
1
0
CGCGAATGCCTCTTAAAAGATCTGAGAGGTCGT
1601












TTGCTGGATTTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2940
+
0
37
1
0
CGCGAATGCCGCTATACCACGTGCCAACCCCTT
1602












ATCTCTGGACAGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
2975
−
0
37
1
0
CGCGAATGCCCACTGAGGGGAAGGGGAGGAAG
1603












TGGCCACCTAGCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3010
+
0
37
1
0
CGCGAATGCCCTACAGGTAGCAATGCTATTGAG
1604












GAAGAGGGTGACACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3045
−
0
37
1
0
CGCGAATGCCGGTGTCATTGCTGAGTTGAAAAT
1605












TACTTCAATGTAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3080
+
0
37
1
0
CGCGAATGCCAGCCATGGCTCTTGCTGACAGTG
1606












CCATTCGCTATGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3115
−
0
37
1
0
CGCGAATGCCTGGGTCGACCACATAGATTCGAC
1607












CTGTTTCAGCATACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3150
+
0
37
1
0
CGCGAATGCCTTTTCTGAGTGCTGTATGGATATT
1608












TCTCTCTCCCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3185
−
0
37
1
0
CGCGAATGCCGCCTAGCTACAGGTGGTGGTTCA
1609












GAACATCGGCTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3220
+
0
37
1
0
CGCGAATGCCTGCTGCAGGAAGAAGAGCAGGA
1610












GAGAAGACGCCCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3255
−
0
37
1
0
CGCGAATGCCCTGGCTGCTGCAAAGAAACTTTG
1611












AGAACGGCTTTGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3290
+
0
37
1
0
CGCGAATGCCAGCCGCTGTCTCTGCTTTTCCAAC
1612












AGACAGCCTCGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3325
−
0
37
1
0
CGCGAATGCCGACAGCCGGGGCTGAGGATGGG
1613












GAAAGGTCTCTCTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3360
+
0
37
1
0
CGCGAATGCCGCTTCGGCTGCAGAGCCGACTTT
1614












AGCCCTCAGCCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3395
−
0
37
1
0
CGCGAATGCCGGGCTGCGGCGAGCGCGGAGGC
1615












CGCAGCTACAACTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3430
+
0
37
1
0
CGCGAATGCCCGGGCATCGGCGCAGCAGCCGCA
1616












GCTGCTGGATTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3465
−
0
37
1
0
CGCGAATGCCGCAACAGGTTGAAACCAGCGGGC
1617












AGAGGCGGAGTCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3500
+
0
37
1
0
CGCGAATGCCTAATGCTGCTGATGCCGAAGCAG
1618












TAAGGGGAGCCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3535
−
0
37
1
0
CGCGAATGCCGTGGGCTCCAGGGTTCGAGCCAC
1619












CGGCAACGTCTTACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3570
+
0
37
1
0
CGCGAATGCCAACCCATCTGCAAACCTTGCCAG
1620












AGGTGATAACCAACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3605
−
0
37
1
0
CGCGAATGCCCCGGAGCGGCAGCTGCAGCGGCA
1621












GCCCCGCCAGCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3640
+
0
37
1
0
CGCGAATGCCAACCACCACCTCGCAGTCGCCGG
1622












GTGCCAAGACCCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3675
−
0
37
1
0
CGCGAATGCCGTGTCGTCGTCGTTGTCAGAATCT
1623












TCTCTCTCCGGACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3710
+
0
37
1
0
CGCGAATGCCTCACGTGAGAATGGATTTTGCCA
1624












GACGTGATAATCACGCGTGGCGGATGT


IRS4
NM_003604
1
X
−
3745
−
0
37
1
0
CGCGAATGCCCTAAAATTACCGACCTCTTTTGG
1625












GAGAGTCGAACTACGCGTGGCGGATGT


RET
NM_020630
1
10
+
−34
+
0
37
1
0
CGCGAATGCCGTCCCTCCAGCCGTGGCCCCAGC
1626












GCGCACGGGCGAACGCGTGGCGGATGT


RET
NM_020630
1
10
+
1
−
0
37
1
0
CGCGAATGCCACGCAGCCCCGCGGCACCGGACG
1627












TCGCCTTCGCCAACGCGTGGCGGATGT


RET
NM_020630
1
10
+
40
+
25
37
1
0
CGCGAATGCCTGTTGCTGCTGCTGCTGCCGCTGC
1628












TAGGCAAAGGTACGCGTGGCGGATGT


RET
NM_020630
1
10
+
71
−
0
37
1
0
CGCGAATGCCCCCCTGCGGGAGCCGGCGGCCGG
1629












CAGAACTCACCTACGCGTGGCGGATGT


EPHA3
NM_005233
14
3
+
0
+
0
37
1
0
CGCGAATGCCGGAGGGAAGATCCCAATCAGGTG
1630












GACATCACCAGAACGCGTGGCGGATGT


EPHA3
NM_005233
14
3
+
35
−
0
37
1
0
CGCGAATGCCCGCTGGCTGACGTGAACTTGCGG
1631












TAGGCTATAGCTACGCGTGGCGGATGT


EPHA3
NM_005233
14
3
+
70
+
0
37
1
0
CGCGAATGCCATGTATGGAGTTATGGGATTGTT
1632












CTCTGGGAGGTGACGCGTGGCGGATGT


EPHA3
NM_005233
14
3
+
105
−
0
37
1
0
CGCGAATGCCGACATCTCCCAGTATGGTCTCTCT
1633












CCATAAGACATACGCGTGGCGGATGT


EPHA3
NM_005233
14
3
+
140
+
0
37
1
0
CGCGAATGCCCAATCAGGATGTAAGTATTTGTG
1634












GTCTATGAGTTAACGCGTGGCGGATGT


RB1
NM_000321
2
13
+
−6
+
0
37
1
0
CGCGAATGCCTGGTAGGCTTGAGTTTGAAGAAA
1635












CAGAAGAACCTGACGCGTGGCGGATGT


RB1
NM_000321
2
13
+
29
−
0
37
1
0
CGCGAATGCCTGGTATCTTTAATTTCTGACATAA
1636












TGCAGTAAAATACGCGTGGCGGATGT


RB1
NM_000321
2
13
+
64
+
0
37
1
0
CGCGAATGCCGATCATGTCAGAGAGAGAGCTTG
1637












GTTAACTTGGGAACGCGTGGCGGATGT


RB1
NM_000321
2
13
+
99
−
0
37
1
0
CGCGAATGCCTCCTTACCAATACTCCATCCACA
1638












GATGAAACTTTCACGCGTGGCGGATGT


RB1
NM_000321
7
13
+
−14
+
0
37
1
0
CGCGAATGCCCATTTTTTTTTCAGGGGAAGTATT
1639












ACAAATGGAAGACGCGTGGCGGATGT


RB1
NM_000321
7
13
+
21
−
0
37
1
0
CGCGAATGCCACATAGCATTAACTGAAATGAAA
1640












TCACCAGATCATACGCGTGGCGGATGT


RB1
NM_000321
7
13
+
56
+
0
37
1
0
CGCGAATGCCGTCCTTGACTATTTTATTAAACTC
1641












TCACCTCCCATACGCGTGGCGGATGT


RB1
NM_000321
7
13
+
91
−
0
37
1
0
CGCGAATGCCAAATTAAATACTTACTATATGGT
1642












TCTTTGAGCAACACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
0
+
0
37
1
0
CGCGAATGCCGTTTCACCGTTGTTTGGAACCATC
1643












TACAATTCAATACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
35
−
0
37
1
0
CGCGAATGCCTCATAGCATTATTCATGGCTTGTG
1644












CGATAAAGTAAACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
70
+
0
37
1
0
CGCGAATGCCAAGAAAATGGACAGGCTGGTGCT
1645












GCCAGCCTGGTTACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
105
−
0
37
1
0
CGCGAATGCCTTGAATCCATGGAACTGCATGTT
1646












TCTGGAATGCTGACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
140
+
0
37
1
0
CGCGAATGCCCCAGTTGATGAGGACAGATTCAA
1647












ATGGAAATGGAAACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
175
−
0
37
1
0
CGCGAATGCCTTTCAAGTTGGTGTCCAGGATTA
1648












CATATTCTGAAAACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
210
+
0
37
1
0
CGCGAATGCCGAATGGGAACTCCATAGCACCTA
1649












CACTGTGGACATACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
245
−
0
37
1
0
CGCGAATGCCTAGGGGTCCCTCCGAAACGTAGC
1650












AGCTCCATTTCCACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
280
+
0
37
1
0
CGCGAATGCCTTCACTTCCCTGGTGGCAGGCCC
1651












CCTAGAGCAGATACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
315
−
0
37
1
0
CGCGAATGCCTGGCAGATCTTCCCTTCTGCAAA
1652












CCAGCATTTTGCACGCGTGGCGGATGT


GUCY2F
NM_001522
17
X
−
350
+
0
37
1
0
CGCGAATGCCTGGAGGTAAGGAATGCAAAATCA
1653












CTAGTAGTCAATACGCGTGGCGGATGT


EPHA4
NM_004438
13
2
−
−8
+
0
37
1
0
CGCGAATGCCTCTAACAGCACCATCATCCATTG
1654












CTTTGGTCCAGGACGCGTGGCGGATGT


EPHA4
NM_004438
13
2
−
27
−
0
37
1
0
CGCGAATGCCAGCCAGTGCCACACTGTATCTTG
1655












TGACTTCTTTAGACGCGTGGCGGATGT


EPHA4
NM_004438
13
2
−
62
+
0
37
1
0
CGCGAATGCCTGGCTGGAACCAGATCGGCCCAA
1656












TGGGGTAATCCTACGCGTGGCGGATGT


EPHA4
NM_004438
13
2
−
97
−
0
37
1
0
CGCGAATGCCGTAATACCTTCTCATAATACTTGA
1657












CTTCATATTCCACGCGTGGCGGATGT


GUCY2F
NM_001522
15
X
−
−22
+
0
37
1
0
CGCGAATGCCTCTGATTTCATGTTTGTTTTAGGC
1658












GTCGTATAAATACGCGTGGCGGATGT


GUCY2F
NM_001522
15
X
−
13
−
0
37
1
0
CGCGAATGCCAGAATTCTATTGGGTCCTTTGATC
1659












AACTGGATTTTACGCGTGGCGGATGT


GUCY2F
NM_001522
15
X
−
48
+
0
37
1
0
CGCGAATGCCACTGACTTTGGAGGATGTAACGT
1660












TTATCAATCCCCACGCGTGGCGGATGT


GUCY2F
NM_001522
15
X
−
83
−
0
37
1
0
CGCGAATGCCAAAGAAGAGGATACTCCTTACCT
1661












TACTGCCAAAGTACGCGTGGCGGATGT


GUCY2F
NM_001522
12
X
−
0
+
0
37
1
0
CGCGAATGCCATGAAGGACTTGCGTCATGAGAA
1662












TATTAACCCTTTACGCGTGGCGGATGT


GUCY2F
NM_001522
12
X
−
35
−
0
37
1
0
CGCGAATGCCTGGCAAACATCCCCGAATCATAG
1663












AAGAAACCCAATACGCGTGGCGGATGT


GUCY2F
NM_001522
12
X
−
70
+
0
37
1
0
CGCGAATGCCTTGTGACAGAATTCTGTTCCCGA
1664












GGGAGCCTAGAAACGCGTGGCGGATGT


GUCY2F
NM_001522
12
X
−
105
−
0
37
1
0
CGCGAATGCCCAGTCAAGTTTCACATCTTGATTT
1665












GTCAGTATGTCACGCGTGGCGGATGT


GUCY2F
NM_001522
12
X
−
140
+
0
37
1
0
CGCGAATGCCGATGTTTAAATCATCACTCTTGCT
1666












GGATCTCATAAACGCGTGGCGGATGT


GUCY2F
NM_001522
12
X
−
175
−
0
37
1
0
CGCGAATGCCAAAATCCAAGATTTCCTCAGTCT
1667












TCCCATTAACCTACGCGTGGCGGATGT


KSR2
NM_173598
9
12
−
−34
+
0
37
1
0
CGCGAATGCCTTTTTTTTCTGAGTGTTCAATCTC
1668












TGGTTTTCAGAACGCGTGGCGGATGT


KSR2
NM_173598
9
12
−
1
−
0
37
1
0
CGCGAATGCCGCGCCCGGGTCGGCGTCTCCGGC
1669












ACCGGCACCACAACGCGTGGCGGATGT


KSR2
NM_173598
9
12
−
36
+
0
37
1
0
CGCGAATGCCCCCAGGTCATCCTGCATCCGGTG
1670












ACCTCGAATCCAACGCGTGGCGGATGT


KSR2
NM_173598
9
12
−
71
−
0
37
1
0
CGCGAATGCCGGGAAAGGGGGAAGATGTCTCTG
1671












TCTCACTTACATACGCGTGGCGGATGT


RBBP8
NM_002894
16
18
+
−35
+
0
37
1
0
CGCGAATGCCACATTTAGTTTGTAATGTGCATGT
1672












TTTATTTATAGACGCGTGGCGGATGT


RBBP8
NM_002894
16
18
+
0
−
0
37
1
0
CGCGAATGCCTTTCTGCTTGACTTTGTCTTGTTT
1673












ATCACCATGAGACGCGTGGCGGATGT


RBBP8
NM_002894
16
18
+
35
+
0
37
1
0
CGCGAATGCCGCGTTTGTGGAGCCGTATTTTAA
1674












AGGTGATGAAAGACGCGTGGCGGATGT


RBBP8
NM_002894
16
18
+
70
−
0
37
1
0
CGCGAATGCCAGTCACATTGCTGGTATAAATAA
1675












AAACCAACTTACACGCGTGGCGGATGT


PDGFRA
NM_006206
18
4
+
−8
+
0
37
1
0
CGCGAATGCCCCATGCAGTGTGTCCACCGTGAT
1676












CTGGCTGCTCGCACGCGTGGCGGATGT


PDGFRA
NM_006206
18
4
+
27
−
0
37
1
0
CGCGAATGCCATCTTCACAATTTTTCCTTGTGCC
1677












AGGAGGACGTTACGCGTGGCGGATGT


PDGFRA
NM_006206
18
4
+
62
+
0
37
1
0
CGCGAATGCCCTGTGACTTTGGCCTGGCCAGAG
1678












ACATCATGCATGACGCGTGGCGGATGT


PDGFRA
NM_006206
18
4
+
97
−
0
37
1
0
CGCGAATGCCAGGACGTACACTGCCTTTCGACA
1679












CATAGTTCGAATACGCGTGGCGGATGT


RPS6KA1
NM_002953
22
1
+
−8
+
0
37
1
0
CGCGAATGCCTCTTTCAGGGAGCCATGGCTGCC
1680












ACGTACTCCGCAACGCGTGGCGGATGT


RPS6KA1
NM_002953
22
1
+
27
−
0
37
1
0
CGCGAATGCCGGCTTCAGCTGGGGGGTGGGCTT
1681












GGAGCTGTTGAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
22
1
+
62
+
0
37
1
0
CGCGAATGCCCATCGAGTCATCCATCCTGGCCC
1682












AGCGGCGAGTGAACGCGTGGCGGATGT


RPS6KA1
NM_002953
22
1
+
97
−
0
37
1
0
CGCGAATGCCCCTGGTGCCTCACAGGGTGGTGG
1683












ATGGCAACTTCCACGCGTGGCGGATGT


EPHA3
NM_005233
1
3
+
−26
+
0
37
1
0
CGCGAATGCCCTCTCACTGCCCTCTGCACCAGC
1684












AACATGGATTGTACGCGTGGCGGATGT


EPHA3
NM_005233
1
3
+
9
−
0
37
1
0
CGCGAATGCCACAGAGCAGCTGAGAAGGAGGA
1685












GGATGGAGAGCTGACGCGTGGCGGATGT


EPHA3
NM_005233
1
3
+
44
+
0
37
1
0
CGCGAATGCCTCTCGACAGCTTCGGGGAACTGA
1686












TTCCGCAGCCTTACGCGTGGCGGATGT


EPHA3
NM_005233
1
3
+
79
−
0
37
1
0
CGCGAATGCCTCCGTGCGTCGCGGTACCTGGCT
1687












TACCTTCATTGGACGCGTGGCGGATGT


PIK3CA
NM_006218
16
3
+
−9
+
0
37
1
0
CGCGAATGCCATTTTAAAGGCTTGAAGAGTGTC
1688












GAATTATGTCCTACGCGTGGCGGATGT


PIK3CA
NM_006218
16
3
+
26
−
0
37
1
0
CGCGAATGCCGTTCTCCCAATTCAACCACAGTG
1689












GCCTTTTTGCAGACGCGTGGCGGATGT


PIK3CA
NM_006218
16
3
+
61
+
0
37
1
0
CGCGAATGCCCCAGACATCATGTCAGAGTTACT
1690












GTTTCAGAACAAACGCGTGGCGGATGT


PIK3CA
NM_006218
16
3
+
96
−
0
37
1
0
CGCGAATGCCCTTCCTTACCATCCCCATTTTTAA
1691












AGATGATCTCAACGCGTGGCGGATGT


EPHB1
NM_004441
4
3
+
0
+
0
37
1
0
CGCGAATGCCCTTGCCCTGCAGGGACATTCAAG
1692












GCCAGCCAGGAAACGCGTGGCGGATGT


EPHB1
NM_004441
4
3
+
35
−
0
37
1
0
CGCGAATGCCCGGCTGTTGGAGGGGCAGTGGGA
1693












GCAGCCTTCAGCACGCGTGGCGGATGT


EPHB1
NM_004441
4
3
+
70
+
0
37
1
0
CGCGAATGCCCTCCCCTGCAGAGGCGTCTCCCA
1694












TCTGCACCTGTCACGCGTGGCGGATGT


EPHB1
NM_004441
4
3
+
105
−
0
37
1
0
CGCGAATGCCTGGAGGGTCAAAGTCCGCTCGGT
1695












AATAACCGGTCCACGCGTGGCGGATGT


EPHB1
NM_004441
4
3
+
140
+
0
37
1
0
CGCGAATGCCGAAGTGGCATGCACTAGTAAGTG
1696












TCTAGTAATGGCACGCGTGGCGGATGT


KSR2
NM_173598
2
12
−
−3
+
0
37
1
0
CGCGAATGCCTAGGACATTCTTCTCTTCTGCTGG
1697












GCCTTTGAACAACGCGTGGCGGATGT


KSR2
NM_173598
2
12
−
32
−
0
37
1
0
CGCGAATGCCTGTCCATGAGCTTGGTGAAGGTA
1698












GGTCTCTCTTCTACGCGTGGCGGATGT


KSR2
NM_173598
2
12
−
67
+
0
37
1
0
CGCGAATGCCTGCTGGAGAAACTGCCAAAGCGA
1699












AACCGTCGCCTGACGCGTGGCGGATGT


KSR2
NM_173598
2
12
−
102
−
0
37
1
0
CGCGAATGCCTACTCTGCAGACTTCCAGAAATG
1700












TCCAGGGTGAGAACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
0
+
0
37
1
0
CGCGAATGCCGAGGCAACCTTTCCAAACAAGAC
1701












TGGACCATCCAGACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
35
−
0
37
1
0
CGCGAATGCCGGATTGTTCTCCTTCCCCGTCTCT
1702












GTCGTGGGCCAACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
70
+
0
37
1
0
CGCGAATGCCCGTGTGCCCCCCGGAGCCCACCC
1703












CGTGGATCCGCAACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
105
−
0
37
1
0
CGCGAATGCCCTTGGACGGGACCCTGGGGCTCT
1704












GGGAGAGATGGGACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
140
+
0
37
1
0
CGCGAATGCCTGCGTCCAGCACTATTGTCACAC
1705












CAGCCCCACTCCACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
175
−
0
37
1
0
CGCGAATGCCTAAGCCTGTCCACGTGGGTGTAC
1706












ACAGGGGCCCCGACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
210
+
0
37
1
0
CGCGAATGCCCCGTGGACGCCTACCCGGGCTTG
1707












TGCCCGCCCCCGACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
245
−
0
37
1
0
CGCGAATGCCGATGGGGGCAGGGAACGGTGGC
1708












CCGACTCCAGTGGACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
280
+
0
37
1
0
CGCGAATGCCGCCCCGGCAGCGGCACGCGGTCC
1709












GCACCCCGCCGCACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
315
−
0
37
1
0
CGCGAATGCCCGGCGGGGTCACGGTGGTGACGA
1710












TGTTGGGGGTGCACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
350
+
0
37
1
0
CGCGAATGCCGGCACGCCGCCCATGAGGAAGA
1711












AGAACAAGCTGAAACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
385
−
0
37
1
0
CGCGAATGCCGTTTTCGGGAGGAGGGCGGTGGG
1712












GTCCCCGGGGGCACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
420
+
0
37
1
0
CGCGAATGCCTGATACACTTGATCCCGGGATTC
1713












ACCGCGCTGCATACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
455
−
0
37
1
0
CGCGAATGCCCGGTGCCCCAGCTGGAACTCGTG
1714












GGATTTGCTCCGACGCGTGGCGGATGT


KSR2
NM_173598
17
12
−
490
+
0
37
1
0
CGCGAATGCCCGTGGACGAGGCCCACACGCCCA
1715












AGTGAGTGCGATACGCGTGGCGGATGT


KSR2
NM_173598
10
12
−
−49
+
0
37
1
0
CGCGAATGCCCGATGTCTCCCTGGGGACACTCA
1716












CTTTGATGTTTTACGCGTGGCGGATGT


KSR2
NM_173598
10
12
−
−14
−
0
37
1
0
CGCGAATGCCTGTATTTGTAGTAGTGGGATGCTT
1717












GCAAAAGAAAGACGCGTGGCGGATGT


KSR2
NM_173598
10
12
−
21
+
0
37
1
0
CGCGAATGCCAGCAGCAGTTCATCTTCCCAGGT
1718












GAGTTTCATGTGACGCGTGGCGGATGT


KSR2
NM_173598
10
12
−
56
−
0
37
1
0
CGCGAATGCCCAGCGGGGCTTGTTCAGAAGGGG
1719












CTGCTTCCAGCTACGCGTGGCGGATGT


EPHA3
NM_005233
8
3
+
−18
+
0
37
1
0
CGCGAATGCCTTCTCTTCAACCTCACAGCTTTCT
1720












CCATCTCTGGTACGCGTGGCGGATGT


EPHA3
NM_005233
8
3
+
17
−
0
37
1
0
CGCGAATGCCGCTGAAATGGCGATCATGACCAC
1721












TTGGCTACTTTCACGCGTGGCGGATGT


EPHA3
NM_005233
8
3
+
52
+
0
37
1
0
CGCGAATGCCGGCAGTAGCAATTATTCTCCTCA
1722












CTGTTGTCATCTACGCGTGGCGGATGT


EPHA3
NM_005233
8
3
+
87
−
0
37
1
0
CGCGAATGCCAAACAGACTGTGAACTCACCTCC
1723












CAATCAAAACATACGCGTGGCGGATGT


KSR2
NM_173598
19
12
−
0
+
0
37
1
0
CGCGAATGCCAGCAAGCTGGTGAAGTACTTCAG
1724












CCGGCAGCTGTCACGCGTGGCGGATGT


KSR2
NM_173598
19
12
−
35
−
0
37
1
0
CGCGAATGCCCGTTGCGCTCCTGCAAGGCTACC
1725












TTCTTTTTGCAGACGCGTGGCGGATGT


KSR2
NM_173598
19
12
−
70
+
0
37
1
0
CGCGAATGCCCGGAGCTGGACGGCTTCCCCCAG
1726












CTACGGCACTGGACGCGTGGCGGATGT


KSR2
NM_173598
19
12
−
105
−
0
37
1
0
CGCGAATGCCTCCAGGACCTCCTTGCGCACATC
1727












GACGATTCGGAAACGCGTGGCGGATGT


KSR2
NM_173598
19
12
−
140
+
0
37
1
0
CGCGAATGCCGGTGACCTGGGGGGCCCCTGTGT
1728












CCCCTGCCCCTTACGCGTGGCGGATGT


NTRK3
NM_001012338
1
15
−
0
+
0
37
1
0
CGCGAATGCCGTCATTGAGTGCATTACCCAAGG
1729












TCGTGTTTTGGAACGCGTGGCGGATGT


NTRK3
NM_001012338
1
15
−
35
−
0
37
1
0
CGCGAATGCCCATCGTACACCTCTTTGGGGCAG
1730












ACTCGGGGCCGCACGCGTGGCGGATGT


NTRK3
NM_001012338
1
15
−
70
+
0
37
1
0
CGCGAATGCCTCATGCTGGGGTGCTGGCAGAGG
1731












GAACCACAGCAGACGCGTGGCGGATGT


NTRK3
NM_001012338
1
15
−
105
−
0
37
1
0
CGCGAATGCCTGGAGGATTTTGTAGATCTCCTTG
1732












ATGTTCAACCGACGCGTGGCGGATGT


NTRK3
NM_001012338
1
15
−
140
+
0
37
1
0
CGCGAATGCCTGCTTTGGGGAAGGCCACCCCAA
1733












TCTACCTGGACAACGCGTGGCGGATGT


NTRK3
NM_001012338
1
15
−
175
−
0
37
1
0
CGCGAATGCCTGAATTCATGACCACCAGCCACC
1734












ACTAGCCAAGAAACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
0
+
0
37
1
0
CGCGAATGCCGGTGTGCTGGGCGATGCCAGGAG
1735












TGGGAAGTCATCACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
35
−
0
37
1
0
CGCGAATGCCCCTGGTATGAGCCAGTCAGGAAT
1736












CGGTGGATGAGCACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
70
+
0
37
1
0
CGCGAATGCCTGCTGGAGAAGACAGAGAGTGA
1737












GTTCTGAAGAGCCACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
105
−
0
37
1
0
CGCGAATGCCTCCTGGATCCCTGACCCCAATGG
1738












TTAGCTTACTATACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
140
+
0
37
1
0
CGCGAATGCCGTGAGATCAATGATTAGGCAGGT
1739












GTGGAGACCAGGACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
175
−
0
37
1
0
CGCGAATGCCCCAGTGCTTTTGCTATTATGGCCT
1740












CATCCTTCCCCACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
210
+
0
37
1
0
CGCGAATGCCGAGCATGGGGAGGTTTGGTGACC
1741












TGTCACCTCTGAACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
245
−
0
37
1
0
CGCGAATGCCCTCACCTGTCCAGAAGAGTTGGA
1742












AGGGGTAACAGGACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
280
+
0
37
1
0
CGCGAATGCCCAGTACAAGAAAGAAATGTTGGT
1743












GGATGGACAGACACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
315
−
0
37
1
0
CGCGAATGCCGTGCCCCAGCTTCCTCTCGGATTA
1744












GCACCAGATGTACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
350
+
0
37
1
0
CGCGAATGCCCTGATGCCAAGGTGAGGGTGGAG
1745












GTGGTGGGGGACACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
385
−
0
37
1
0
CGCGAATGCCCCTCTCCTTTGGCCAGTGCTGACC
1746












CCAGCCAGCCTACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
420
+
0
37
1
0
CGCGAATGCCGCTCTGGTTTCCCAGGTCTTCATC
1747












TGGATTGGGTCACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
455
−
0
37
1
0
CGCGAATGCCGAGCAAATGGAAAGTCAGACAG
1748












GTCTACTCCTCTGACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
490
+
0
37
1
0
CGCGAATGCCCCTCACTCCCCGCAAGTTCTCAG
1749












GCTGGGCAGATGACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
525
−
0
37
1
0
CGCGAATGCCGTTCTCATCCTCCAGGCTGAAGA
1750












CGAAGATCACAGACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
560
+
0
37
1
0
CGCGAATGCCAGTTTCCAGGCTGTGAGCCGTCT
1751












CCATGGGCAGCTACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
595
−
0
37
1
0
CGCGAATGCCCCAGGCCTCCTCGTCCCTCCCCGC
1752












GAAGGGAACTCACGCGTGGCGGATGT


CENTG1
NM_014770
16
12
−
630
+
0
37
1
0
CGCGAATGCCCCTTGGCACTGGTGGGGACACAA
1753












GGTAAGGAGGGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
3
1
+
−12
+
0
37
1
0
CGCGAATGCCTCCCTCCATCAGGATGAGGGCGT
1754












CCTCAAGGAGATACGCGTGGCGGATGT


RPS6KA1
NM_002953
3
1
+
23
−
0
37
1
0
CGCGAATGCCTCTCAGAGCCAGCCTTGACGTGG
1755












TGCGTGATGGAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
3
1
+
58
+
0
37
1
0
CGCGAATGCCAGGCTGATCCATCCCATTTCGAG
1756












CTCCTCAAGGTTACGCGTGGCGGATGT


RPS6KA1
NM_002953
3
1
+
93
−
0
37
1
0
CGCGAATGCCTCATGACTCACTTTGCCAAAGGA
1757












TCCCTGGCCCAGACGCGTGGCGGATGT


PIK3CA
NM_006218
14
3
+
0
+
0
23
1
1
CGCGAATGCCATCTGAGATGCACAATAAAACAG
1758












TTAGCCAGAGGTACGCGTGGCGGATGT


PIK3CA
NM_006218
14
3
+
35
−
0
0
2
0
CGCGAATGCCACATGCACGACAATAGGACTCCA
1759












AAAGCAGGCCAAACGCGTGGCGGATGT


PIK3CA
NM_006218
14
3
+
70
+
0
0
2
0
CGCGAATGCCGGGATGTATTTGAAGCACCTGAA
1760












TAGGCAAGTCGAACGCGTGGCGGATGT


PIK3CA
NM_006218
14
3
+
105
−
0
23
1
1
CGCGAATGCCGAATGTCAGTTAAGTTAATGAGC
1761












TTTTCCATTGCCACGCGTGGCGGATGT


PIK3CA
NM_006218
14
3
+
139
+
0
37
1
0
CGCGAATGCCCTCAAACAGGAGAAGAAGGATG
1762












AAACACAAAAGGTACGCGTGGCGGATGT


EPHA7
NM_004440
7
6
−
0
+
0
37
1
0
CGCGAATGCCGAGAATTCGGTGAAGTCTGCAGT
1763












GGCCGTTTGAAAACGCGTGGCGGATGT


EPHA7
NM_004440
7
6
−
35
−
0
37
1
0
CGCGAATGCCTTTATGGCTACTGCAACATCTCTT
1764












TTCCCTGGAAGACGCGTGGCGGATGT


EPHA7
NM_004440
7
6
−
70
+
0
37
1
0
CGCGAATGCCAACCCTGAAAGTTGGTTACACAG
1765












AAAAACAAAGGAACGCGTGGCGGATGT


EPHA7
NM_004440
7
6
−
105
−
0
37
1
0
CGCGAATGCCCTGCCCCATGATGCTTGCTTCACA
1766












CAAAAAGTCTCACGCGTGGCGGATGT


EPHA7
NM_004440
7
6
−
140
+
0
37
1
0
CGCGAATGCCTTTGACCACCCGAATGTTGTCCAT
1767












TTGGAAGGGGTACGCGTGGCGGATGT


EPHA7
NM_004440
7
6
−
175
−
0
37
1
0
CGCGAATGCCAATAAAGATATAACCAATATCTA
1768












CCTCTTGTAACAACGCGTGGCGGATGT


CENTG1
NM_014770
18
12
−
0
+
0
37
1
0
CGCGAATGCCATGCATGCCCAGAGGCAGTTCGT
1769












TGTAGCTGCAGTACGCGTGGCGGATGT


CENTG1
NM_014770
18
12
−
35
−
0
37
1
0
CGCGAATGCCGCTTGGCCACCTCATGTCGTCTG
1770












ACTTCTGCTCTCACGCGTGGCGGATGT


CENTG1
NM_014770
18
12
−
70
+
0
37
1
0
CGCGAATGCCAGGCTCTAAACCGCCTCAGGAAG
1771












CTGGCAGAGAGGACGCGTGGCGGATGT


CENTG1
NM_014770
18
12
−
105
−
0
37
1
0
CGCGAATGCCGCCTGGATGCTGTCCTGGAGTTC
1772












GGGGTCGTCCACACGCGTGGCGGATGT


CENTG1
NM_014770
18
12
−
140
+
0
37
1
0
CGCGAATGCCCTCATTGGACAGCATTCGAGGTA
1773












AGAGAAAGGTCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
0
+
0
37
1
0
CGCGAATGCCATGGCGAGCCCTCCGGAGAGCGA
1774












TGGCTTCTCGGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
35
−
0
37
1
0
CGCGAATGCCTCTTGGGTTTGCGCAGGTAGCCC
1775












ACCTTGCGCACGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
70
+
0
37
1
0
CGCGAATGCCGCATGCACAAACGCTTCTTCGTA
1776












CTGCGCGCGGCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
105
−
0
37
1
0
CGCGAATGCCTAGTACTCGAGGCGCGCCGGGCC
1777












CCCAGCCTCGCTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
140
+
0
37
1
0
CGCGAATGCCCGAGAACGAGAAGAAGTGGCGG
1778












CACAAGTCGAGCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
175
−
0
37
1
0
CGCGAATGCCGAAGCAGCTCTCAAGGGGGATCG
1779












AGCGTTTGGGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
210
+
0
37
1
0
CGCGAATGCCAACATCAACAAGCGGGCTGACTC
1780












CAAGAACAAGCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
245
−
0
37
1
0
CGCGAATGCCCAAAGTGCTCGTCCCGGGTGTAG
1781












AGAGCCACCAGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
280
+
0
37
1
0
CGCGAATGCCCCATCGCGGCGGACAGCGAGGCC
1782












GAGCAAGACAGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
315
−
0
37
1
0
CGCGAATGCCGCACGGTTGTGCAGCTGTAGGAG
1783












AGCCTGGTACCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
350
+
0
37
1
0
CGCGAATGCCTAAGGGCCACCACGACGGAGCTG
1784












CGGCCCTCGGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
385
−
0
37
1
0
CGCGAATGCCGGAGCTGCCGCTGCAGCTGCCCC
1785












CACCACCTCCCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
420
+
0
37
1
0
CGCGAATGCCGGCCTTGGTGAGGCTGGGGAGGA
1786












CTTGAGCTACGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
455
−
0
37
1
0
CGCGAATGCCAGACCTCTTTGAATGCGGGTCCT
1787












GGGGGCACGTCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
490
+
0
37
1
0
CGCGAATGCCGGCAAGTGATCCTGAAGCCCAAG
1788












GGCCTGGGTCAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
525
−
0
37
1
0
CGCGAATGCCAGGCAAAGGCGGTAGATACCAAT
1789












CAGGTTCTTTGTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
560
+
0
37
1
0
CGCGAATGCCGACCAGCAAGACCATCAGCTTCG
1790












TGAAGCTGAACTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
595
−
0
37
1
0
CGCGAATGCCGTTCATCAGCTGCAGCACCACGG
1791












CCGCTGCCTCCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
630
+
0
37
1
0
CGCGAATGCCATCAGGCGCTGTGGCCACTCGGA
1792












AAACTTCTTCTTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
665
−
0
37
1
0
CGCGAATGCCCGGGCCCCGTCACGGCAGAACGG
1793












CCCACCTCGATGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
700
+
0
37
1
0
CGCGAATGCCGGGAGTTCTGGATGCAGGTGGAT
1794












GACTCTGTGGTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
735
−
0
37
1
0
CGCGAATGCCATGGCCTCCAGGATGGTCTCGTG
1795












CATGTTCTGGGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
770
+
0
37
1
0
CGCGAATGCCGCGGGCCATGAGTGATGAGTTCC
1796












GCCCTCGCAGCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
805
−
0
37
1
0
CGCGAATGCCGATGGGGTTAGAGCAGTTGGACG
1797












AGGACTGGCTCTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
840
+
0
37
1
0
CGCGAATGCCAGCGTCCCCCTGCGCCGGCACCA
1798












TCTCAACAATCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
875
−
0
37
1
0
CGCGAATGCCGTGATCGGCGGGTCAGCCCCACC
1799












TGGCTGGGCGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
910
+
0
37
1
0
CGCGAATGCCGCACTGAGAGCATCACCGCCACC
1800












TCCCCGGCCAGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
945
−
0
37
1
0
CGCGAATGCCCGGACACGGAAGGAGCCTGGCTT
1801












CCCGCCCACCATACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
980
+
0
37
1
0
CGCGAATGCCCGCCTCCAGTGACGGCGAAGGCA
1802












CCATGTCCCGCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1015
−
0
37
1
0
CGCGAATGCCGCTGGGACTCACAGGGCTGCCGT
1803












CCACCGAGGCTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1050
+
0
37
1
0
CGCGAATGCCACCAACAGAACCCACGCCCACCG
1804












GCATCGGGGCAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1085
−
0
37
1
0
CGCGAATGCCAGCGGCTGTGGTTGAGCGGGGGG
1805












TGCAGCCGGGCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1120
+
0
37
1
0
CGCGAATGCCCCATCCCCATGCCGGCTTCCCGCT
1806












GCTCGCCTTCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1155
−
0
37
1
0
CGCGAATGCCGTGCTACTGGACGACAGACTGAC
1807












CGGGCTGGTGGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1190
+
0
37
1
0
CGCGAATGCCCAGTGGCCATGGCTCCACCTCGG
1808












ATTGTCTCTTCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1225
−
0
37
1
0
CGCGAATGCCGGGGGAACCAGACACCGAAGCA
1809












CTAGATCGCCGTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1260
+
0
37
1
0
CGCGAATGCCAGCGATGGCGGTTTCATCTCCTC
1810












GGATGAGTATGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1295
−
0
37
1
0
CGCGAATGCCTGCGGAAGGAACTCCGGAAATCG
1811












CAGGGACTGGAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1330
+
0
37
1
0
CGCGAATGCCGTGTCACTCCGGATTCCCTGGGC
1812












CACACCCCACCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1365
−
0
37
1
0
CGCGAATGCCCAGATATAGTTGCTTAGCTCCTCC
1813












TCACCGCGGGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1400
+
0
37
1
0
CGCGAATGCCCATGGGTGGCAAGGGGCCCTCCA
1814












CCCTGACCGCCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1435
−
0
37
1
0
CGCGAATGCCATTGCCACCCCGAGACAAAATGT
1815












AGTGACCGTTGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1470
+
0
37
1
0
CGCGAATGCCGGCCACCGCTGCACCCCAGGAAC
1816












AGGCTTGGGCACACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1505
−
0
37
1
0
CGCGAATGCCCACTGGCTGCTTCATCCCCAGCC
1817












AAGGCTGGACTCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1540
+
0
37
1
0
CGCGAATGCCCTGCAGATCTGGATAATCGGTTC
1818












CGAAAGAGAACTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1575
−
0
37
1
0
CGCGAATGCCTGGTGGGTAATGGTAGGGGATGT
1819












GCCTGCCGAGTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1610
+
0
37
1
0
CGCGAATGCCGAAGACCCCGTCCCAGTCCTCAG
1820












TGGCTTCCATTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1645
−
0
37
1
0
CGCGAATGCCTGGTGGGTAGGCAGGCATCATCT
1821












CTGTGTACTCCTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1680
+
0
37
1
0
CGCGAATGCCGGAGGTGGCAGTGGAGGCCGACT
1822












GCCGGGACACAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1715
−
0
37
1
0
CGCGAATGCCCTGGGTAGGAGCGGGTGGGCACG
1823












AAGGCGGAGTGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1750
+
0
37
1
0
CGCGAATGCCAGGAGGGTCTGGAAATGCACCCC
1824












TTGGAGCGTCGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1785
−
0
37
1
0
CGCGAATGCCTGGAGGGTGGAGCTGTCTGGGCG
1825












GTGGTGCCCCCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1820
+
0
37
1
0
CGCGAATGCCCACGGATGATGGCTACATGCCCA
1826












TGTCCCCAGGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1855
−
0
37
1
0
CGCGAATGCCTCCACTGCCCTTTCGGCCACTGG
1827












GCACTGGGGCCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1890
+
0
37
1
0
CGCGAATGCCGACTATATGCCCATGAGCCCCAA
1828












GAGCGTATCTGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1925
−
0
37
1
0
CGCGAATGCCGATGGCGTCTGATGGGATTGATG
1829












ATCTGCTGTGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1960
+
0
37
1
0
CGCGAATGCCCCCAGAGAGTGGACCCCAATGGC
1830












TACATGATGATGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
1995
−
0
37
1
0
CGCGAATGCCCCTCCAATGTCAGGAGAGCAGCC
1831












ACCGCTGGGGGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2030
+
23
37
1
0
CGCGAATGCCTGGCCCCAGCAGCAGCAGCAGCA
1832












GCAGCAACGCCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2065
−
0
37
1
0
CGCGAATGCCTGTCCACAGCTTTCCATAGCTGGT
1833












CCCGGAAGGGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2100
+
0
37
1
0
CGCGAATGCCAACGGGGTAGGGGGCCACCACTC
1834












TCATGTCTTGCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2135
−
0
37
1
0
CGCGAATGCCTACCACCGCTGCTCTCCACTGGG
1835












GGTTTGGGGTGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2170
+
0
37
1
0
CGCGAATGCCAGCTCTTACCTTGCACAGGTGAC
1836












TACATGAACATGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2205
−
0
37
1
0
CGCGAATGCCGAGGGGCTGCTGGTGTTGGAGTC
1837












CCCCACTGGTGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2240
+
0
37
1
0
CGCGAATGCCCGACTGCTACTACGGCCCTGAGG
1838












ACCCCCAGCACAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2275
−
0
37
1
0
CGCGAATGCCGGATCTTGGCAATGAGTAGTAGG
1839












AGAGGACTGGCTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2310
+
0
37
1
0
CGCGAATGCCTTTAAGCACACCCAGCGCCCCGG
1840












GGAGCCGGAGGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2345
−
0
37
1
0
CGCGAATGCCTAGTGGAAAGGCGGAGGTGCTGA
1841












TGCCGGGCACCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2380
+
0
37
1
0
CGCGAATGCCGCTCTGGTCGCCTTCTCTATGCTG
1842












CAACAGCAGATACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2415
−
0
37
1
0
CGCGAATGCCCCCAGGCTGTCGCTGCTGGTGGA
1843












AGAGGAAGAATCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2450
+
0
37
1
0
CGCGAATGCCTGGGGGATACTGCGGGGCTAGGC
1844












TGGAGCCCAGCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2485
−
0
37
1
0
CGCGAATGCCATGGGGCTGCAGAACCTGATGGT
1845












GGGGATGTGGAAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2520
+
0
37
1
0
CGCGAATGCCCTGCCTCGAAAGGTGGACACAGC
1846












TGCTCAGACCAAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2555
−
0
37
1
0
CGCGAATGCCCCAGGGACAGCCTCGTGGGCCGG
1847












GCCAGGCGGCTAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2590
+
0
37
1
0
CGCGAATGCCGGGATCCCAAGGCCAGCACCTTA
1848












CCTCGGGCCCGAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2629
−
18
37
1
0
CGCGAATGCCTGGAGGGTGCAGCAAGGGCTGCT
1849












GCTGCTGCTGCTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2660
+
0
37
1
0
CGCGAATGCCTCCAGAGCCCAAGAGCCCGGGGG
1850












AATATGTCAATAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2695
−
0
37
1
0
CGCGAATGCCAGACAAGTAGCCAGACTGATCAC
1851












TCCCAAATTCAAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2730
+
0
37
1
0
CGCGAATGCCGGCCCGGTGGCTTTCCACAGCTC
1852












ACCTTCTGTCAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2765
−
0
37
1
0
CGCGAATGCCCCTCTCTGGGAGCTGGCTGGAGC
1853












TGGGATGGACACACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2800
+
0
37
1
0
CGCGAATGCCAAGAGACTGGCACTGAGGAGTAC
1854












ATGAAGATGGACACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2835
−
0
37
1
0
CGCGAATGCCCTCTCCTGCCAGGCTGCCCTCCG
1855












GCCCGGCCCCAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2870
+
0
37
1
0
CGCGAATGCCCACTGGGGTCGAGATGGGCAGAC
1856












TGGGCCCTGCACACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2905
−
0
37
1
0
CGCGAATGCCCCGGGTAGGCCTGCAAATGCTAG
1857












CAGCCCCGGGAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2940
+
0
37
1
0
CGCGAATGCCGCAGTGCCCAGCAGCCGGGGTGA
1858












CTACATGACCATACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
2975
−
0
37
1
0
CGCGAATGCCTGTCCACGTAGCTCTGACGGGGA
1859












CAACTCATCTGCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3010
+
0
37
1
0
CGCGAATGCCCCTCGCCAGCTGCCCCTGTAAGC
1860












TATGCTGACATGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3045
−
0
37
1
0
CGCGAATGCCGGCAGGCTCACCTCCTCTGCAGC
1861












AATGCCTGTTCGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3080
+
0
37
1
0
CGCGAATGCCCAGGGCCACCATGGCTGCTGCCT
1862












CCTCATCCTCAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3115
−
0
37
1
0
CGCGAATGCCTGCCCCTTGAGGCCCAGTCGGGG
1863












AAGCAGAGGCTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3150
+
0
37
1
0
CGCGAATGCCGCAGAGCTGGCTGCCCACTCGTC
1864












CCTGCTGGGGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3185
−
0
37
1
0
CGCGAATGCCGGGTGAAGGCGCTCATGCCCCCA
1865












GGTCCTTGTGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3220
+
0
37
1
0
CGCGAATGCCGGGTGAACCTCAGTCCTAACCGC
1866












AACCAGAGTGCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3255
−
0
37
1
0
CGCGAATGCCCGCCGGCACCCTTGTGGGTCTGC
1867












ACGGATCACTTTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3290
+
0
37
1
0
CGCGAATGCCGAGGCATAGCTCCGAGACTTTCT
1868












CCTCAACACCCAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3325
−
0
37
1
0
CGCGAATGCCTCCAAAGGGCACTGTGTTGCCCA
1869












CCCGGGTGGCACACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3360
+
0
37
1
0
CGCGAATGCCGCGGGGGCAGCAGTAGGGGGCG
1870












GTGGCGGTAGCAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3395
−
0
37
1
0
CGCGAATGCCCAGAGCTGTGGCGTTTCACATCC
1871












TCGCTGCTGCTGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3430
+
0
37
1
0
CGCGAATGCCCTTTCCTTTGAGAATGTGTGGCTG
1872












AGGCCTGGGGAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3465
−
0
37
1
0
CGCGAATGCCCACAGTTTGGCTGGCTCCTTGGG
1873












GGCTCCCCCAAGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3500
+
0
37
1
0
CGCGAATGCCTGGGGCTGCTGGGGGTTTGGAGA
1874












ATGGTCTTAACTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3535
−
0
37
1
0
CGCGAATGCCCTGTTTGAAGTCCTTGACCAAAT
1875












CCAGGTCTATGTACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3570
+
0
37
1
0
CGCGAATGCCTGCCCTCAGGAGTGCACCCCTGA
1876












ACCGCAGCCTCCACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3605
−
0
37
1
0
CGCGAATGCCCGCTGCCCAGGGGTTGATGAGGG
1877












GGTGGGGGTGGGACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3640
+
0
37
1
0
CGCGAATGCCGTGAGAGCAGCTCCACCCGCCGC
1878












TCAAGTGAGGATACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3675
−
0
37
1
0
CGCGAATGCCTGCTTCTGGAAACTGATGCTGGC
1879












ATAGGCGCTTAAACGCGTGGCGGATGT


IRS1
NM_005544
2
2
−
3710
+
0
37
1
0
CGCGAATGCCGCCAGAGGACCGTCAGTAGCTCA
1880












ACTGGACATCACACGCGTGGCGGATGT


RBBP8
NM_002894
3
18
+
−48
+
0
37
1
0
CGCGAATGCCCATAAAGGAACTGTTGTAGAAGT
1881












AATACCTTTTCTACGCGTGGCGGATGT


RBBP8
NM_002894
3
18
+
−13
−
0
37
1
0
CGCGAATGCCTTGGTTACTTTTACTTGTAAACCT
1882












GAAAAGTAAAAACGCGTGGCGGATGT


RBBP8
NM_002894
3
18
+
22
+
0
37
1
0
CGCGAATGCCGCTAAAACAGGAACGAATCTTGT
1883












AAGTATCAGTATACGCGTGGCGGATGT


RBBP8
NM_002894
3
18
+
57
−
0
37
1
0
CGCGAATGCCTATCAACTACTCTATAAATAACA
1884












CATGAGTATTACACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
0
+
0
37
1
0
CGCGAATGCCATCCCCCACGTGTGGTGAGCCTG
1885












GAGGAGCCTGAGACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
35
−
0
37
1
0
CGCGAATGCCCGCACCACAAACTCGATGCAGTG
1886












CTCCAGGCGCAGACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
70
+
0
37
1
0
CGCGAATGCCTGGCAACCCCCCACCAACGCTGC
1887












ACTGGCTGCACAACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
105
−
0
37
1
0
CGCGAATGCCATGGATGATCTTGGACTCCCGCA
1888












GAGGCTGCCCATACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
140
+
0
37
1
0
CGCGAATGCCGTGGAATACTACCAAGAGGGAG
1889












AGATTTCCGAGGGACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
175
−
0
37
1
0
CGCGAATGCCTGTTGTAGTGGGTGGGCTTGTTG
1890












AAGAGCAGGCAGACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
210
+
0
37
1
0
CGCGAATGCCATGGCAACTATACCCTCATTGCC
1891












AAAAACCCACTGACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
245
−
0
37
1
0
CGCGAATGCCAGGAAGTGGCCATTGATGGTCTG
1892












GTTGGCTGTGCCACGCGTGGCGGATGT


NTRK3
NM_001012338
11
15
−
280
+
0
37
1
0
CGCGAATGCCCAAGGAGCCCTTTCCAGGTGAGG
1893












GCAGCGTAGCTGACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
0
+
0
37
1
0
CGCGAATGCCGTGGCACCCCTTACCCCGGCATG
1894












ATGGTGGATTCTACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
35
−
0
37
1
0
CGCGAATGCCATCCGGTACCCACTCTTGATCTTA
1895












TTGTAGAAAGTACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
70
+
0
37
1
0
CGCGAATGCCGGCCAAGCCTGACCACGCTACCA
1896












GTGAAGTGTGAGACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
105
−
0
37
1
0
CGCGAATGCCGACTGTGAACACAGGCCCCCGGG
1897












ATGGGGAAGGAGACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
140
+
0
37
1
0
CGCGAATGCCTGTGGGTCTAGGGGGAGGGAGG
1898












GGCCCTGAGACTTACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
175
−
0
37
1
0
CGCGAATGCCCTGTGGGGACAGAACTCAAGAGT
1899












GGGCACAGGGGGACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
210
+
0
37
1
0
CGCGAATGCCCTACGAGATCATGGTGAAATGCT
1900












GGAACAGTGAGCACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
245
−
0
37
1
0
CGCGAATGCCCTCACTCAGGTGGTAAAAGGAGG
1901












GTCTCTTCTCCGACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
280
+
0
37
1
0
CGCGAATGCCATTGTGGAGAATCTGCTGCCTGG
1902












ACAATATAAAAAACGCGTGGCGGATGT


PDGFRA
NM_006206
21
4
+
315
−
0
37
1
0
CGCGAATGCCATCCAGACCTTTCCACCCACAGA
1903












TCCAAACACACCACGCGTGGCGGATGT


PDGFRA
NM_006206
9
4
+
−6
+
0
37
1
0
CGCGAATGCCTTGCAGTTCCTTCATCCATTCTGG
1904












ACTTGGTCGATACGCGTGGCGGATGT


PDGFRA
NM_006206
9
4
+
29
−
0
37
1
0
CGCGAATGCCCTCACCGTCTGTCCCCCAGTTGA
1905












GCCATGGTGATCACGCGTGGCGGATGT


PDGFRA
NM_006206
9
4
+
64
+
0
37
1
0
CGCGAATGCCGTGCACAGCTGAAGGCACGCCGC
1906












TTCCTGATATTGACGCGTGGCGGATGT


PDGFRA
NM_006206
9
4
+
99
−
0
37
1
0
CGCGAATGCCTCCATACTTCTTAATATCTTTGCA
1907












TATCATCCACTACGCGTGGCGGATGT


PDGFRA
NM_006206
13
4
+
−18
+
0
37
1
0
CGCGAATGCCGATTCTGCCTGCCCACAGGTCGG
1908












GTCTTGGGGTCTACGCGTGGCGGATGT


PDGFRA
NM_006206
13
4
+
17
−
0
37
1
0
CGCGAATGCCTAGGCTGTTCCTTCAACCACCTTC
1909












CCAAACGCTCCACGCGTGGCGGATGT


PDGFRA
NM_006206
13
4
+
52
+
0
37
1
0
CGCGAATGCCTGGATTAAGCCGGTCCCAACCTG
1910












TCATGAAAGTTGACGCGTGGCGGATGT


PDGFRA
NM_006206
13
4
+
87
−
0
37
1
0
CGCGAATGCCCAGGAAGGAGCACTTACGTTTTA
1911












GCATCTTCACTGACGCGTGGCGGATGT


GUCY2F
NM_001522
13
X
−
−25
+
0
37
1
0
CGCGAATGCCTTCATTCTCCTCCTTTTCTTTCTAG
1912












GGTGATTGGGACGCGTGGCGGATGT


GUCY2F
NM_001522
13
X
−
10
−
0
37
1
0
CGCGAATGCCTCCAAAATCTCCAAGGGAGAACT
1913












TTTTCAGCCACAACGCGTGGCGGATGT


GUCY2F
NM_001522
13
X
−
45
+
0
37
1
0
CGCGAATGCCGACCTTAAGTCCATCAAATCAAG
1914












AGCAAGTGATGTACGCGTGGCGGATGT


GUCY2F
NM_001522
13
X
−
80
−
0
37
1
0
CGCGAATGCCTTACATAAACGAAGATTTCTCAT
1915












ACCATTTCGAACACGCGTGGCGGATGT


NTRK3
NM_001012338
17
15
−
−32
+
0
37
1
0
CGCGAATGCCGGCTGACCGTTACCTTTCCTCCTC
1916












CCCTGCAGACAACGCGTGGCGGATGT


NTRK3
NM_001012338
17
15
−
3
−
0
37
1
0
CGCGAATGCCCGTTGAGCGTGTGAAGACTGCGC
1917












CAGTTCTCTATGACGCGTGGCGGATGT


NTRK3
NM_001012338
17
15
−
38
+
0
37
1
0
CGCGAATGCCCCGTGGACATGGAGCTCTACACC
1918












GGACTTCAAAAGACGCGTGGCGGATGT


NTRK3
NM_001012338
17
15
−
73
−
0
37
1
0
CGCGAATGCCGGGAAAGGCCTCTCTGTGGCCGG
1919












GTGTACTCACAGACGCGTGGCGGATGT


CHAF1A
NM_005483
2
19
+
−44
+
0
37
1
0
CGCGAATGCCTTTGCAGAATTTAACAGTTTACCC
1920












TTTGACACTTTACGCGTGGCGGATGT


CHAF1A
NM_005483
2
19
+
−9
−
0
37
1
0
CGCGAATGCCAGCTGGTCTATCTTTGCAATCCAT
1921












GGCTGCAAATAACGCGTGGCGGATGT


CHAF1A
NM_005483
2
19
+
26
+
0
37
1
0
CGCGAATGCCTTTCCAGTTAAGAAGTTAATACA
1922












AGGTAATTATTTACGCGTGGCGGATGT


CHAF1A
NM_005483
2
19
+
61
−
0
37
1
0
CGCGAATGCCACTATAAACTACGAACAACTCTT
1923












TAACCCATTTCCACGCGTGGCGGATGT


PKN1
NM_002741
5
19
+
0
+
0
37
1
0
CGCGAATGCCGGAGTCCTGACCTGGGGGCTGTG
1924












GAGCTGCGCATCACGCGTGGCGGATGT


PKN1
NM_002741
5
19
+
35
−
0
37
1
0
CGCGAATGCCGCGTGCTCCACTCGGAAGTGGTG
1925












CCGCAGCTCTTCACGCGTGGCGGATGT


PKN1
NM_002741
5
19
+
70
+
0
37
1
0
CGCGAATGCCGGTGGCCGAGGGTGCCAAGAAC
1926












GTACTGCGCCTGCACGCGTGGCGGATGT


PKN1
NM_002741
5
19
+
105
−
0
37
1
0
CGCGAATGCCGACTGCCTTGCGGTCCGGGGCCT
1927












TGGCAGCGCTGAACGCGTGGCGGATGT


PKN1
NM_002741
5
19
+
140
+
0
37
1
0
CGCGAATGCCAGCGAGGTGAGGGGCGGAGCTTT
1928












CATTAGAGGCGGACGCGTGGCGGATGT


KSR2
NM_173598
20
12
−
−24
+
0
37
1
0
CGCGAATGCCCAGCAGTGCGAACTGGTCCAAAA
1929












CATGATAGACTTACGCGTGGCGGATGT


KSR2
NM_173598
20
12
−
11
−
0
37
1
0
CGCGAATGCCATTTGGTCCTAAGCCCTTCCAGGT
1930












TGGAGATGCTCACGCGTGGCGGATGT


KSR2
NM_173598
20
12
−
46
+
0
37
1
0
CGCGAATGCCGTGCTACCTCCAACGACCTCACA
1931












CAAAAAGAAATCACGCGTGGCGGATGT


KSR2
NM_173598
20
12
−
77
−
1
37
1
0
CGCGAATGCCTCCCGTAGGCAACACCTACCTCC
1932












AGGGTCCGGATTACGCGTGGCGGATGT


EPHA7
NM_004440
17
6
−
−22
+
0
37
1
0
CGCGAATGCCAAATAAAACCTGCTCATGCACCA
1933












TGGTTTTTCAAAACGCGTGGCGGATGT


EPHA7
NM_004440
17
6
−
13
−
0
37
1
0
CGCGAATGCCGATGTAGCATAAAATAATCCATG
1934












AAGGGTACCGAGACGCGTGGCGGATGT


EPHA7
NM_004440
17
6
−
48
+
0
37
1
0
CGCGAATGCCTGGCTGCTCCGCTTTGCACACAC
1935












AGGGGAGGCGCAACGCGTGGCGGATGT


EPHA7
NM_004440
17
6
−
83
−
0
37
1
0
CGCGAATGCCTTTGCTTTCCCATCACCTTACCTT
1936












CCTTCGCAGCCACGCGTGGCGGATGT


NFKB1
NM_003998
16
4
+
−12
+
0
37
1
0
CGCGAATGCCTTTTCATTCCAGTGTCTTACACTT
1937












AGCAATCATCCACGCGTGGCGGATGT


NFKB1
NM_003998
16
4
+
23
−
0
37
1
0
CGCGAATGCCTTCTAGTAGATCCCTCACAAGTT
1938












GAGAATGAAGGTACGCGTGGCGGATGT


NFKB1
NM_003998
16
4
+
58
+
0
37
1
0
CGCGAATGCCGTCACATCTGGTTTGATTTCTGAT
1939












GACATTATCAAACGCGTGGCGGATGT


NFKB1
NM_003998
16
4
+
93
−
0
37
1
0
CGCGAATGCCGATTTCTGCTTACCTGGTACAGAT
1940












CATTTCTCATGACGCGTGGCGGATGT


EPHA4
NM_004438
15
2
−
0
+
0
37
1
0
CGCGAATGCCCTTGCAAAATTGGATATTACAAG
1941












GCTCTCTCCACGACGCGTGGCGGATGT


EPHA4
NM_004438
15
2
−
35
−
0
37
1
0
CGCGAATGCCTAGCTGTGGGGTGGGCACTTGGC
1942












ACAGGTGGCATCACGCGTGGCGGATGT


EPHA4
NM_004438
15
2
−
70
+
0
37
1
0
CGCGAATGCCCTCTGTCTGGGAAGGAGCCACCT
1943












CGTGCACCTGTGACGCGTGGCGGATGT


EPHA4
NM_004438
15
2
−
105
−
0
37
1
0
CGCGAATGCCGGCAGCATCGTTGTCAGCTCTGA
1944












AAAAGCCTCGGTACGCGTGGCGGATGT


EPHA4
NM_004438
15
2
−
140
+
0
37
1
0
CGCGAATGCCTCTATGCCCTGCACCCGTAAGTT
1945












GTATGCTTGTCTACGCGTGGCGGATGT


RB1
NM_000321
11
13
+
−31
+
0
37
1
0
CGCGAATGCCTATGTGAATGACTTCACTTATTGT
1946












TATTTAGTTTTACGCGTGGCGGATGT


RB1
NM_000321
11
13
+
4
−
0
37
1
0
CGCGAATGCCTCAAGGTTACTTTTTCGTGGTGTT
1947












CTCTGTGTTTCACGCGTGGCGGATGT


RB1
NM_000321
11
13
+
39
+
0
37
1
0
CGCGAATGCCTGAAGAGGTGAATGTAATTCCTC
1948












CACACACTCCAGACGCGTGGCGGATGT


RB1
NM_000321
11
13
+
70
−
11
37
1
0
CGCGAATGCCTATAATTAAAAGTAGGAAAATTC
1949












ATACCTAACTGGACGCGTGGCGGATGT


EPHA4
NM_004438
8
2
−
0
+
0
37
1
0
CGCGAATGCCGTGAATTTGGTGAGGTATGCAGT
1950












GGGCGTCTCAAAACGCGTGGCGGATGT


EPHA4
NM_004438
8
2
−
35
−
0
37
1
0
CGCGAATGCCTTGATAGCCACACAGATCTCTCT
1951












CTTGCCAGGCACACGCGTGGCGGATGT


EPHA4
NM_004438
8
2
−
70
+
0
37
1
0
CGCGAATGCCGACTCTGAAAGCTGGTTATACAG
1952












ACAAACAGAGGAACGCGTGGCGGATGT


EPHA4
NM_004438
8
2
−
105
−
0
37
1
0
CGCGAATGCCCTGTCCCATGATGCTGGCCTCACT
1953












CAGGAAGTCTCACGCGTGGCGGATGT


EPHA4
NM_004438
8
2
−
140
+
0
37
1
0
CGCGAATGCCTTTGACCATCCGAACATCATTCA
1954












CTTGGAAGGCGTACGCGTGGCGGATGT


EPHA4
NM_004438
8
2
−
175
−
0
37
1
0
CGCGAATGCCTTGTAAAGGGGGTGACCCACGTA
1955












CATTTAGTGACCACGCGTGGCGGATGT


RB1
NM_000321
14
13
+
−42
+
0
37
1
0
CGCGAATGCCCTAAAATAGCAGGCTCTTATTTTT
1956












CTTTTTGTTTGACGCGTGGCGGATGT


RB1
NM_000321
14
13
+
−7
−
0
37
1
0
CGCGAATGCCAATACAAGCGAACTCCAAGTTTG
1957












TATCGCTACAAAACGCGTGGCGGATGT


RB1
NM_000321
14
13
+
28
+
0
37
1
0
CGCGAATGCCACCGAGTAATGGAATCCATGCTT
1958












AAATCAGTAAGTACGCGTGGCGGATGT


RB1
NM_000321
14
13
+
59
−
8
37
1
0
CGCGAATGCCGCCCGGCTGAAATTTTTTTATATT
1959












GTTTTTAACTTACGCGTGGCGGATGT


PALB2
NM_024675
13
16
−
−46
+
0
37
1
0
CGCGAATGCCGCCTGGGGTCGGCGACGGCTGCT
1960












CTTTTCGTTCTGACGCGTGGCGGATGT


PALB2
NM_024675
13
16
−
−11
−
0
37
1
0
CGCGAATGCCGGGCTTCCCGGGAGGCTCGTCCA
1961












TCGGGCAGGCGAACGCGTGGCGGATGT


PALB2
NM_024675
13
16
−
24
+
0
37
1
0
CGCGAATGCCCTCAGCTGTGAGGAGAAGGAAA
1962












AGGTGCCGGGGGTACGCGTGGCGGATGT


PALB2
NM_024675
13
16
−
59
−
0
37
1
0
CGCGAATGCCAAGCGGGGTCAGAGTCCTGCGTC
1963












CGCCCTTCCCGCACGCGTGGCGGATGT


EPHA3
NM_005233
12
3
+
−39
+
0
37
1
0
CGCGAATGCCACTGTACTGATTATTATTTATTAT
1964












TTACTGTATATACGCGTGGCGGATGT


EPHA3
NM_005233
12
3
+
−4
−
0
37
1
0
CGCGAATGCCATGTATTCTGTGACAATCATAAC
1965












TGGCTTACCTAGACGCGTGGCGGATGT


EPHA3
NM_005233
12
3
+
31
+
0
37
1
0
CGCGAATGCCGGAGAATGGTTCCTTGGATAGTT
1966












TCCTACGTGTAAACGCGTGGCGGATGT


EPHA3
NM_005233
12
3
+
66
−
0
37
1
0
CGCGAATGCCATTTATTCATATATATGTATGTGT
1967












GTGCATCTTACACGCGTGGCGGATGT


RB1
NM_000321
13
13
+
−12
+
0
37
1
0
CGCGAATGCCTACCTCCTAAAGAACTGCACAGT
1968












GAATCCAAAAGAACGCGTGGCGGATGT


RB1
NM_000321
13
13
+
23
−
0
37
1
0
CGCGAATGCCTGTATCCTATATCCTTCACTCTTT
1969












TCAGTATACTTACGCGTGGCGGATGT


RB1
NM_000321
13
13
+
58
+
0
37
1
0
CGCGAATGCCTCTTTAAAGAGAAATTTGCTAAA
1970












GCTGTGGGACAGACGCGTGGCGGATGT


RB1
NM_000321
13
13
+
93
−
0
37
1
0
CGCGAATGCCATTCAAGTTACCTGTGATCCAATT
1971












TCGACACAACCACGCGTGGCGGATGT


RPS6KA1
NM_002953
2
1
+
−48
+
0
37
1
0
CGCGAATGCCGCGTGTAAGTTCTGACAGTGCTC
1972












CCCCAATCTCCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
2
1
+
−13
−
0
37
1
0
CGCGAATGCCCTTCCCCTGAGGTCTGTCCATTCT
1973












GGAAAAGAGAAACGCGTGGCGGATGT


RPS6KA1
NM_002953
2
1
+
22
+
0
37
1
0
CGCGAATGCCAAGCTGGACTTCAGCCGTCCAAG
1974












GTGAGGACCATGACGCGTGGCGGATGT


RPS6KA1
NM_002953
2
1
+
57
−
0
37
1
0
CGCGAATGCCAGGATCCCCCACAGCCCCTCGCT
1975












CAGGGTGCTGGCACGCGTGGCGGATGT


RB1
NM_000321
6
13
+
−36
+
0
37
1
0
CGCGAATGCCTTCCTGTTTTTTTTCTGCTTTCTAT
1976












TTGTTTAATAACGCGTGGCGGATGT


RB1
NM_000321
6
13
+
−1
−
0
37
1
0
CGCGAATGCCTAGCACCAATGCAGAATTTATTT
1977












CAGTAGATATCCACGCGTGGCGGATGT


RB1
NM_000321
6
13
+
34
+
0
37
1
0
CGCGAATGCCAAAGTTTCTTGGATCACATTTTTA
1978












TTAGCTAAAGGACGCGTGGCGGATGT


RB1
NM_000321
6
13
+
69
−
0
37
1
0
CGCGAATGCCTGAAATATTAGCATTTAATAAAT
1979












ATAATGAACTTAACGCGTGGCGGATGT


CHAF1A
NM_005483
15
19
+
−20
+
0
37
1
0
CGCGAATGCCCTCCCTCTCTTGCCCTGCAGAGGT
1980












CCAAGCCCCGTACGCGTGGCGGATGT


CHAF1A
NM_005483
15
19
+
15
−
0
37
1
0
CGCGAATGCCCCCCACACCACCCCCAGCTCCGG
1981












AAGCGGCTCCACACGCGTGGCGGATGT


CHAF1A
NM_005483
15
19
+
50
+
0
37
1
0
CGCGAATGCCGTGGACACCGGCAAGGCCACCCT
1982












GACCTCGAGCCCACGCGTGGCGGATGT


CHAF1A
NM_005483
15
19
+
85
−
0
37
1
0
CGCGAATGCCACATACGTCACCCCTGCTCTCAG
1983












GATGCACCCAGTACGCGTGGCGGATGT


RET
NM_020630
18
10
+
−20
+
0
37
1
0
CGCGAATGCCCTGTCTGCTCTTCCCACCAGGTAC
1984












CGCCTGATGCTACGCGTGGCGGATGT


RET
NM_020630
18
10
+
15
−
0
37
1
0
CGCGAATGCCCCGGCCTTTTGTCCGGCTCCTGCT
1985












TCCAGCATTGCACGCGTGGCGGATGT


RET
NM_020630
18
10
+
50
+
0
37
1
0
CGCGAATGCCTGTTTGCGGACATCAGCAAAGAC
1986












CTGGAGAAGATGACGCGTGGCGGATGT


RET
NM_020630
18
10
+
85
−
0
37
1
0
CGCGAATGCCAATTGGACCCAGGCACTCACTCT
1987












CCTCTTAACCATACGCGTGGCGGATGT


NFKB1
NM_003998
17
4
+
0
+
0
37
1
0
CGCGAATGCCACGCCCTTGCACTTGGCAGTGAT
1988












CACTAAGCAGGAACGCGTGGCGGATGT


NFKB1
NM_003998
17
4
+
35
−
0
37
1
0
CGCGAATGCCCGGCCCCAGCCCTCAGCAAATCC
1989












TCCACCACATCTACGCGTGGCGGATGT


NFKB1
NM_003998
17
4
+
70
+
0
37
1
0
CGCGAATGCCACCTGAGCCTTCTGGACCGCTTG
1990












GGTAACTCTGTTACGCGTGGCGGATGT


NFKB1
NM_003998
17
4
+
105
−
0
37
1
0
CGCGAATGCCACTTTATCATGTCCTTCTTTGGCA
1991












GCTAGGTGCAAACGCGTGGCGGATGT


NFKB1
NM_003998
17
4
+
140
+
0
37
1
0
CGCGAATGCCTCTCAGTATCTTACTCAAGCACA
1992












AAAAGGCAGCACACGCGTGGCGGATGT


NFKB1
NM_003998
17
4
+
175
−
0
37
1
0
CGCGAATGCCTCTCTTACCGTCCCCGTTGGGGTG
1993












GTCAAGAAGTAACGCGTGGCGGATGT


NFKB1
NM_003998
5
4
+
−20
+
0
37
1
0
CGCGAATGCCCTTAACGTTCACCTTTGCAGAGA
1994












GGATTTCGTTTCACGCGTGGCGGATGT


NFKB1
NM_003998
5
4
+
15
−
0
37
1
0
CGCGAATGCCAGTCCACCATGGGATGGGCCTTC
1995












ACATACATAACGACGCGTGGCGGATGT


NFKB1
NM_003998
5
4
+
50
+
0
37
1
0
CGCGAATGCCACCTGGTGCCTCTAGTGAAAAGA
1996












ACAAGAAGTCTTACGCGTGGCGGATGT


NFKB1
NM_003998
5
4
+
85
−
0
37
1
0
CGCGAATGCCGGAGAGCTACCACAAACTTACTT
1997












TGACCTGAGGGTACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
0
+
0
37
1
0
CGCGAATGCCGGGAGTTTGGAGAAGTGTACAAG
1998












GGGCGTTTGAAAACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
35
−
0
37
1
0
CGCGAATGCCTTGATGGCCACGTAGATTTCCCTC
1999












TTGCCTGGCAGACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
70
+
0
37
1
0
CGCGAATGCCGACCCTGAAGGCAGGGTACTCGG
2000












AGAAGCAGCGTCACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
105
−
0
37
1
0
CGCGAATGCCCTGGCCCATGATGCTCGCCTCAC
2001












TCAGAAAGTCCCACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
140
+
0
37
1
0
CGCGAATGCCTTCGACCATCCTAACATCATTCGC
2002












CTGGAGGGTGTACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
175
−
0
37
1
0
CGCGAATGCCCTGTGATGATCATGACAGGCCGA
2003












CTCTTGGTGACCACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
210
+
0
37
1
0
CGCGAATGCCAGTTCATGGAGAATGGTGCATTG
2004












GATTCTTTCCTCACGCGTGGCGGATGT


EPHB1
NM_004441
11
3
+
245
−
0
37
1
0
CGCGAATGCCAGTGCATCATCAAACCCCTGAGT
2005












TGCTCTTACCCTACGCGTGGCGGATGT


EPHB1
NM_004441
15
3
+
0
+
0
37
1
0
CGCGAATGCCGCCTTCCCAGCCCCTGCTCGACC
2006












GCTCCATCCCAGACGCGTGGCGGATGT


EPHB1
NM_004441
15
3
+
35
−
0
37
1
0
CGCGAATGCCGAGCCAGTCATCCACGGTGGTAA
2007












AGGCCGTGAAGTACGCGTGGCGGATGT


EPHB1
NM_004441
15
3
+
70
+
0
37
1
0
CGCGAATGCCAGCGCCATCAAAATGGTCCAGTA
2008












CAGGGACAGCTTACGCGTGGCGGATGT


EPHB1
NM_004441
15
3
+
105
−
0
37
1
0
CGCGAATGCCTGACCAGCTGGAGGGAGGTGAA
2009












GCCAGCAGTGAGGACGCGTGGCGGATGT


EPHB1
NM_004441
15
3
+
140
+
0
37
1
0
CGCGAATGCCCCCAGATGACATCAGAGTAAGTG
2010












ATGAGAATCTCTACGCGTGGCGGATGT


PIK3CA
NM_006218
6
3
+
−27
+
0
37
1
0
CGCGAATGCCTAGTATATACCTACTTTTTTCTTT
2011












TAGATCTATGTACGCGTGGCGGATGT


PIK3CA
NM_006218
6
3
+
8
−
0
37
1
0
CGCGAATGCCATAAGGGTTCTCCTCCATGGTAG
2012












ATACCTGTTCGAACGCGTGGCGGATGT


PIK3CA
NM_006218
6
3
+
43
+
0
37
1
0
CGCGAATGCCGTGACAATGTGAACACTCAAAGA
2013












GTACCTTGTTCCACGCGTGGCGGATGT


PIK3CA
NM_006218
6
3
+
78
−
0
37
1
0
CGCGAATGCCGAAATATAAATCTATATACTTCC
2014












TTACCTGGGATTACGCGTGGCGGATGT


NTRK3
NM_001012338
6
15
−
−4
+
0
37
1
0
CGCGAATGCCACAGATGTGCAGCACATTAAGAG
2015












GAGAGACATCGTACGCGTGGCGGATGT


NTRK3
NM_001012338
6
15
−
31
−
0
37
1
0
CGCGAATGCCTTCCAAAGGCTCCCTCACCCAGT
2016












TCTCGCTTCAGCACGCGTGGCGGATGT


NTRK3
NM_001012338
6
15
−
66
+
0
37
1
0
CGCGAATGCCAGGTCTTCCTGGCCGAGTGCTAC
2017












AACCTCAGCCCGACGCGTGGCGGATGT


NTRK3
NM_001012338
6
15
−
101
−
0
37
1
0
CGCGAATGCCTTTACCTTCACAGCCACAAGCAT
2018












CTTGTCCTTGGTACGCGTGGCGGATGT


CHAF1A
NM_005483
14
19
+
−22
+
0
37
1
0
CGCGAATGCCGCCGTGTACCCTGTCTGTCCAGA
2019












TTGGTGCTGAAGACGCGTGGCGGATGT


CHAF1A
NM_005483
14
19
+
9
−
6
37
1
0
CGCGAATGCCTCCTCCGTGTCTGCCTGGAAGCC
2020












GTCCATGTCTTCACGCGTGGCGGATGT


CHAF1A
NM_005483
14
19
+
52
+
9
37
1
0
CGCGAATGCCAGGAGGAGGGCGACTGTATGATC
2021












GTGGATGTCCCGACGCGTGGCGGATGT


CHAF1A
NM_005483
14
19
+
83
−
0
37
1
0
CGCGAATGCCGCTATCTACAGCCCTTCTCACCCG
2022












CAGCATCCGGGACGCGTGGCGGATGT


NFKB1
NM_003998
8
4
+
0
+
0
37
1
0
CGCGAATGCCATCGGGAAAAAGAGCTAATCCGC
2023












CAAGCAGCTCTGACGCGTGGCGGATGT


NFKB1
NM_003998
8
4
+
35
−
0
37
1
0
CGCGAATGCCCGCACCACGCTGAGGTCCATCTC
2024












CTTGGTCTGCTGACGCGTGGCGGATGT


NFKB1
NM_003998
8
4
+
70
+
0
37
1
0
CGCGAATGCCGCTCATGTTTACAGCTTTTCTTCC
2025












GGATAGCACTGACGCGTGGCGGATGT


NFKB1
NM_003998
8
4
+
105
−
0
37
1
0
CGCGAATGCCTGATACCACGGGTTCCAGGCGCC
2026












TTGTGAAGCTGCACGCGTGGCGGATGT


NFKB1
NM_003998
8
4
+
140
+
0
37
1
0
CGCGAATGCCGACGCCATCTATGACAGTAGTGA
2027












GTACTTCACTTCACGCGTGGCGGATGT


RPS6KA1
NM_002953
13
1
+
−18
+
0
37
1
0
CGCGAATGCCGCCCACCCCACTGTGCAGAAGCT
2028












ATACCGTCGTGAACGCGTGGCGGATGT


RPS6KA1
NM_002953
13
1
+
17
−
0
37
1
0
CGCGAATGCCGCTGAGCCACTGCTGGCTTGAAG
2029












GGTGGCTTGATCACGCGTGGCGGATGT


RPS6KA1
NM_002953
13
1
+
52
+
0
37
1
0
CGCGAATGCCCTGATGACACCTTCTACTTTGACA
2030












CCGAGTTCACGACGCGTGGCGGATGT


RPS6KA1
NM_002953
13
1
+
87
−
0
37
1
0
CGCGAATGCCAAACAGATAAGGGACGCACCCTT
2031












GGGTGTGCGGGAACGCGTGGCGGATGT


EPHB1
NM_004441
14
3
+
0
+
0
37
1
0
CGCGAATGCCGTCATCAATGCCATCGAGCAGGA
2032












CTACCGGCTGCCACGCGTGGCGGATGT


EPHB1
NM_004441
14
3
+
35
−
0
37
1
0
CGCGAATGCCGCTGGTGTAGAGCAGCTGGACAG
2033












TCCATGGGTGGGACGCGTGGCGGATGT


EPHB1
NM_004441
14
3
+
70
+
0
37
1
0
CGCGAATGCCTCATGCTGGACTGTTGGCAGAAG
2034












GACCGGAACAGCACGCGTGGCGGATGT


EPHB1
NM_004441
14
3
+
105
−
0
37
1
0
CGCGAATGCCTCTAGGGTGTTGACAATCTCCGC
2035












AAACCGGGGCCGACGCGTGGCGGATGT


EPHB1
NM_004441
14
3
+
140
+
0
37
1
0
CGCGAATGCCTAAGATGATCCGGAACCCGGCAA
2036












GTCTCAAGACTGACGCGTGGCGGATGT


EPHB1
NM_004441
14
3
+
175
−
0
37
1
0
CGCGAATGCCGTTTCACTAGACTCACACGGCGG
2037












TGATGGTTGCCAACGCGTGGCGGATGT


NTRK3
NM_001012338
12
15
−
0
+
0
37
1
0
CGCGAATGCCACCAATCTGAACTGGACCAATGT
2038












TCATGCCATCAAACGCGTGGCGGATGT


NTRK3
NM_001012338
12
15
−
35
−
0
37
1
0
CGCGAATGCCCATTGTCCTCACTCGTCACATTCA
2039












CCAGCGTCAAGACGCGTGGCGGATGT


NTRK3
NM_001012338
12
15
−
70
+
0
37
1
0
CGCGAATGCCGCTTCACCCTGACGTGCATTGCA
2040












GAGAACGTGGTGACGCGTGGCGGATGT


NTRK3
NM_001012338
12
15
−
105
−
0
37
1
0
CGCGAATGCCTAGACAGTGAGGGCAACACTGGC
2041












ATTGCTCATGCCACGCGTGGCGGATGT


NTRK3
NM_001012338
12
15
−
140
+
0
37
1
0
CGCGAATGCCCTGTAAGTGCATGTTATTGTGGG
2042












GGATGGCTGTGTACGCGTGGCGGATGT


EPHA7
NM_004440
4
6
−
0
+
0
37
1
0
CGCGAATGCCGGTGGAAAAATTCCAGTAAGGTG
2043












GACAGCACCCGAACGCGTGGCGGATGT


EPHA7
NM_004440
4
6
−
35
−
0
37
1
0
CGCGAATGCCCACTGGCTGATGTGAATTTCCGG
2044












TACTGGATGGCTACGCGTGGCGGATGT


EPHA7
NM_004440
4
6
−
70
+
0
37
1
0
CGCGAATGCCATGTATGGAGCTATGGAATAGTC
2045












ATGTGGGAAGTTACGCGTGGCGGATGT


EPHA7
NM_004440
4
6
−
105
−
0
37
1
0
CGCGAATGCCGACATGTCCCAATAAGGTCTTTC
2046












TCCATAAGACATACGCGTGGCGGATGT


EPHA7
NM_004440
4
6
−
140
+
0
37
1
0
CGCGAATGCCAAATCAAGATGTAGGTGTTACAT
2047












TCATTTAAACAAACGCGTGGCGGATGT


EPHA7
NM_004440
14
6
−
0
+
0
37
1
0
CGCGAATGCCCCTGTGGCCGTGGGTTCTACAAG
2048












TCTTCCTCTCAAACGCGTGGCGGATGT


EPHA7
NM_004440
14
6
−
35
−
0
37
1
0
CGCGAATGCCAAACTGTGAGTTGGACAACGAGA
2049












GCACTGAAGATCACGCGTGGCGGATGT


EPHA7
NM_004440
14
6
−
70
+
0
37
1
0
CGCGAATGCCTTCTGATAAAGAAGGCTCCTCCA
2050












GATGTGAATGTGACGCGTGGCGGATGT


EPHA7
NM_004440
14
6
−
105
−
0
37
1
0
CGCGAATGCCTGGTGGGTCAGATGGAGCCCTGT
2051












AATACCCATCTTACGCGTGGCGGATGT


EPHA7
NM_004440
14
6
−
140
+
0
37
1
0
CGCGAATGCCTACGTTGCGTGCACAAGTGAGTT
2052












GTATTATGAAAGACGCGTGGCGGATGT


NFKB1
NM_003998
12
4
+
0
+
0
37
1
0
CGCGAATGCCATAAAGAAGAAGTGCAGAGGAA
2053












ACGTCAGAAGCTCACGCGTGGCGGATGT


NFKB1
NM_003998
12
4
+
35
−
0
37
1
0
CGCGAATGCCCTACCACCGCCGAAACTATCCGA
2054












AAAATTGGGCATACGCGTGGCGGATGT


NFKB1
NM_003998
12
4
+
70
+
0
37
1
0
CGCGAATGCCTGGTGCTGGAGCTGGAGGCGGAG
2055












GCATGTTTGGTAACGCGTGGCGGATGT


NFKB1
NM_003998
12
4
+
105
−
0
37
1
0
CGCGAATGCCACCTGTACTTCCAGTGCCCCCTCC
2056












TCCACCGCCACACGCGTGGCGGATGT


NFKB1
NM_003998
12
4
+
140
+
0
37
1
0
CGCGAATGCCCCAGGTACAAAAATACTTATTCT
2057












TCCTAAAACTTTACGCGTGGCGGATGT


NFKB1
NM_003998
21
4
+
−36
+
0
37
1
0
CGCGAATGCCATGTATAACGATTTCTGGTGTTTT
2058












TCTTTCCAACAACGCGTGGCGGATGT


NFKB1
NM_003998
21
4
+
−1
−
0
37
1
0
CGCGAATGCCGCTCATATGGTTTCCCATTTAATA
2059












TGTCAAATACCACGCGTGGCGGATGT


NFKB1
NM_003998
21
4
+
34
+
0
37
1
0
CGCGAATGCCCAGAGTTTACATCTGATGATTTA
2060












CTAGCACAAGGTACGCGTGGCGGATGT


NFKB1
NM_003998
21
4
+
69
−
0
37
1
0
CGCGAATGCCATAATGGTTAAGAGAATCTGGTT
2061












TTATCACAACCCACGCGTGGCGGATGT


EPHA4
NM_004438
7
2
−
−39
+
0
37
1
0
CGCGAATGCCCTCATCAAAGGTTTGTGTTTTTCT
2062












TTCTGAAAACTACGCGTGGCGGATGT


EPHA4
NM_004438
7
2
−
−4
−
0
37
1
0
CGCGAATGCCATGTACTCTGTTATGATCATTACT
2063












GGTTTACCTAGACGCGTGGCGGATGT


EPHA4
NM_004438
7
2
−
31
+
0
37
1
0
CGCGAATGCCGGAGAATGGCTCCTTGGATGCAT
2064












TCCTCAGGGTATACGCGTGGCGGATGT


EPHA4
NM_004438
7
2
−
66
−
0
37
1
0
CGCGAATGCCTACCAACATTCTTGGGTTTAATAT
2065












AAAGTAGTCACACGCGTGGCGGATGT


RET
NM_020630
17
10
+
−1
+
0
37
1
0
CGCGAATGCCGATGGTCTTTTGGTGTCCTGCTGT
2066












GGGAGATCGTGACGCGTGGCGGATGT


RET
NM_020630
17
10
+
34
−
0
37
1
0
CGCGAATGCCGGAGGAATCCCAGGATAGGGGTT
2067












TCCCCCTAGGGTACGCGTGGCGGATGT


RET
NM_020630
17
10
+
69
+
0
37
1
0
CGCGAATGCCTGAGCGGCTCTTCAACCTTCTGA
2068












AGACCGGCCACCACGCGTGGCGGATGT


RET
NM_020630
17
10
+
104
−
0
37
1
0
CGCGAATGCCCATCTCCTCGCTGCAGTTGTCTGG
2069












CCTCTCCATCCACGCGTGGCGGATGT


EPHA4
NM_004438
12
2
−
0
+
0
37
1
0
CGCGAATGCCGATCAGAATGAGCGAAGCTATCG
2070












TATAGTTCGGACACGCGTGGCGGATGT


EPHA4
NM_004438
12
2
−
35
−
0
37
1
0
CGCGAATGCCGGTTCAGGCCTTTGATATCTGTGT
2071












TCCTGGCAGCTACGCGTGGCGGATGT


EPHA4
NM_004438
12
2
−
70
+
0
37
1
0
CGCGAATGCCCTCTCACTTCCTATGTTTTCCACG
2072












TGCGAGCCAGGACGCGTGGCGGATGT


EPHA4
NM_004438
12
2
−
105
−
0
37
1
0
CGCGAATGCCAAGGGCTCACTGAAGTCTCCATA
2073












GCCAGCTGCTGTACGCGTGGCGGATGT


EPHA4
NM_004438
12
2
−
140
+
0
37
1
0
CGCGAATGCCGGAGGTTACAACCAACACAGGTA
2074












ACAAGGACCACCACGCGTGGCGGATGT


KSR2
NM_173598
1
12
−
−66
+
0
37
1
0
CGCGAATGCCACAACGGATGACATCATCATGTC
2075












CTTGTGACCTCTACGCGTGGCGGATGT


KSR2
NM_173598
1
12
−
−31
−
0
37
1
0
CGCGAATGCCCAGCCTGGAGTGGGGAGAGAAG
2076












GGAGAGAGTGGTGACGCGTGGCGGATGT


KSR2
NM_173598
1
12
−
4
+
0
37
1
0
CGCGAATGCCTGACCTTTGGACATCGGGACGGC
2077












GCCCAGCTGCCTACGCGTGGCGGATGT


KSR2
NM_173598
1
12
−
39
−
0
37
1
0
CGCGAATGCCAGGACAGAGTAGGGAGGGAGAG
2078












GTGACGGGAGCCCACGCGTGGCGGATGT


PALB2
NM_024675
7
16
−
0
+
0
37
1
0
CGCGAATGCCAATCCTTCAGGTTCCTGTTCCGTA
2079












GATGTGAGTGCACGCGTGGCGGATGT


PALB2
NM_024675
7
16
−
35
−
0
37
1
0
CGCGAATGCCATGGCTCTTTACAACCGGCTCTTT
2080












CCCAAAACATGACGCGTGGCGGATGT


PALB2
NM_024675
7
16
−
70
+
0
37
1
0
CGCGAATGCCGTATCATAACTGCTTGCGAAGAT
2081












GTAGTTTCTCTTACGCGTGGCGGATGT


PALB2
NM_024675
7
16
−
105
−
0
37
1
0
CGCGAATGCCAGTTTTTCCCACTGCCAAGCATCC
2082












AGAGCTTTCCAACGCGTGGCGGATGT


PALB2
NM_024675
7
16
−
140
+
0
37
1
0
CGCGAATGCCTTATACCTGGCACTTCGCAGAGG
2083












TAAGTGGGAATCACGCGTGGCGGATGT


PIK3CA
NM_006218
18
3
+
0
+
0
37
1
0
CGCGAATGCCAATGTTACCTTATGGTTGTCTGTC
2084












AATCGGTGACTACGCGTGGCGGATGT


PIK3CA
NM_006218
18
3
+
35
−
0
37
1
0
CGCGAATGCCGTGAGAATTTCGCACCACCTCAA
2085












TAAGTCCCACACACGCGTGGCGGATGT


PIK3CA
NM_006218
18
3
+
70
+
0
37
1
0
CGCGAATGCCACTATTATGCAAATTCAGTGCAA
2086












AGGCGGCTTGAAACGCGTGGCGGATGT


PIK3CA
NM_006218
18
3
+
105
−
0
37
1
0
CGCGAATGCCGATGTAGTGTGTGGCTGTTGAAC
2087












TGCAGTGCACCTACGCGTGGCGGATGT


PIK3CA
NM_006218
18
3
+
140
+
0
37
1
0
CGCGAATGCCAGTGGCTCAAAGACAAGAACAA
2088












AGGAGAAATGTGAACGCGTGGCGGATGT


CENTG1
NM_001122772
6
12
−
−36
+
24
37
1
0
CGCGAATGCCCATTCTATCTCTATCTCCTTCGCT
2089












TCGGGAAACCAACGCGTGGCGGATGT


CENTG1
NM_001122772
6
12
−
−5
−
0
37
1
0
CGCGAATGCCAGGATTTTAGTTTCCACATTTTGC
2090












GCTTGGCTGGTACGCGTGGCGGATGT


CENTG1
NM_001122772
6
12
−
30
+
0
37
1
0
CGCGAATGCCTTGGTAGTTTAAGAAATATTTAT
2091












AAAGCAGGTAACACGCGTGGCGGATGT


CENTG1
NM_001122772
6
12
−
65
−
0
37
1
0
CGCGAATGCCCACCCCAGCTGAGCGCCCCTCCC
2092












GGCTCCCCACTTACGCGTGGCGGATGT


EPHA7
NM_004440
12
6
−
−8
+
0
37
1
0
CGCGAATGCCCCAAACAGCTCCCTCGCAAGTGA
2093












GTGGAGTAATGAACGCGTGGCGGATGT


EPHA7
NM_004440
12
6
−
27
−
0
37
1
0
CGCGAATGCCGGAAAGCTCGACACTCCGCTGCA
2094












GTACTCTCTCCTACGCGTGGCGGATGT


EPHA7
NM_004440
12
6
−
62
+
0
37
1
0
CGCGAATGCCTGGCAGGAACCAGAGCATCCCAA
2095












TGGAGTCATCACACGCGTGGCGGATGT


EPHA7
NM_004440
12
6
−
97
−
0
37
1
0
CGCGAATGCCCACTTACTTTCTCGTAATACTTGA
2096












TTTCATATTCTACGCGTGGCGGATGT


PDGFRA
NM_006206
15
4
+
0
+
0
37
1
0
CGCGAATGCCGCCCCATTTACATCATCACAGAG
2097












TATTGCTTCTATACGCGTGGCGGATGT


PDGFRA
NM_006206
15
4
+
35
−
0
37
1
0
CGCGAATGCCTCCCTATTCTTATGCAAATAGTTG
2098












ACCAAATCTCCACGCGTGGCGGATGT


PDGFRA
NM_006206
15
4
+
70
+
0
37
1
0
CGCGAATGCCTAGCTTCCTGAGCCACCACCCAG
2099












AGAAGCCAAAGAACGCGTGGCGGATGT


PDGFRA
NM_006206
15
4
+
105
−
0
37
1
0
CGCGAATGCCATCAGCAGGGTTCAATCCAAAGA
2100












TATCCAGCTCTTACGCGTGGCGGATGT


PDGFRA
NM_006206
15
4
+
140
+
0
37
1
0
CGCGAATGCCGAAAGCACACGGAGGTGGGTGC
2101












AAAGAGAGATGTTACGCGTGGCGGATGT


RET
NM_020630
8
10
+
−7
+
0
37
1
0
CGCGAATGCCCCTGCAGATGTGGCCGAGGAGGC
2102












GGGCTGCCCCCTACGCGTGGCGGATGT


RET
NM_020630
8
10
+
28
−
0
37
1
0
CGCGAATGCCCACACTCCAGCCGTCTCTTGCTG
2103












ACTGCACAGGACACGCGTGGCGGATGT


RET
NM_020630
8
10
+
63
+
0
37
1
0
CGCGAATGCCAGGAGTGTGGCGGCCTGGGCTCC
2104












CCAACAGGCAGGACGCGTGGCGGATGT


RET
NM_020630
8
10
+
98
−
0
37
1
0
CGCGAATGCCGGCTTACCTTTGCCATCTCCTTGC
2105












CTCCACTCACAACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
0
+
0
37
1
0
CGCGAATGCCACCCAGATGTAGCCTTTGTACCT
2106












CTAGGAATGACGACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
35
−
0
37
1
0
CGCGAATGCCGCAGAATCATCATCCTCCACGAT
2107












GACTAAATAATCACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
70
+
0
37
1
0
CGCGAATGCCCATTATACCTTGTCGCACAACTG
2108












ATCCCGAGACTCACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
105
−
0
37
1
0
CGCGAATGCCAGGTACCACCCCCTCACTGTTGT
2109












GTAAGGTTACAGACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
140
+
0
37
1
0
CGCGAATGCCGCCTCCTACGACAGCAGACAGGG
2110












CTTTAATGGGACACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
175
−
0
37
1
0
CGCGAATGCCCGGTGGCCTCACAGATATAGGGC
2111












CCTACAGTGAAGACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
210
+
0
37
1
0
CGCGAATGCCTCAAAGGAAAGAAGTTCCAGACC
2112












ATCCCATTTAATACGCGTGGCGGATGT


PDGFRA
NM_006206
4
4
+
245
−
0
37
1
0
CGCGAATGCCAAGGAGATGATACAAGTACCTTT
2113












TAAAGCATAAACACGCGTGGCGGATGT


RET
NM_020630
15
10
+
−8
+
0
37
1
0
CGCGAATGCCCCTCACAGCTCGTTCATCGGGAC
2114












TTGGCAGCCAGAACGCGTGGCGGATGT


RET
NM_020630
15
10
+
27
−
0
37
1
0
CGCGAATGCCATCTTCATCTTCCGCCCCTCAGCT
2115












ACCAGGATGTTACGCGTGGCGGATGT


RET
NM_020630
15
10
+
62
+
0
37
1
0
CGCGAATGCCTTCGGATTTCGGCTTGTCCCGAG
2116












ATGTTTATGAAGACGCGTGGCGGATGT


RET
NM_020630
15
10
+
97
−
0
37
1
0
CGCGAATGCCACTGGGCACCTGGCTCCTCTTCA
2117












CGTAGGAATCCTACGCGTGGCGGATGT


RPS6KA1
NM_002953
19
1
+
−32
+
0
37
1
0
CGCGAATGCCCAGACTGACCACCTCCCCTGCCC
2118












TGTTGCCAGGTGACGCGTGGCGGATGT


RPS6KA1
NM_002953
19
1
+
3
−
0
37
1
0
CGCGAATGCCATGTCGCAGCCTTCATCGTAGCC
2119












CTGGCGCTTCAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
19
1
+
38
+
0
37
1
0
CGCGAATGCCCTGGAGCCTGGGCATTCTGCTGT
2120












ACACCATGCTGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
19
1
+
73
−
0
37
1
0
CGCGAATGCCGTGGGGAAGGGTCCAGGCCAGG
2121












GGCACTCACCCTGACGCGTGGCGGATGT


RB1
NM_000321
16
13
+
−2
+
0
37
1
0
CGCGAATGCCAGGAAGAAGAACGATTATCCATT
2122












CAAAATTTTAGGACGCGTGGCGGATGT


RB1
NM_000321
16
13
+
31
−
33
37
1
0
CGCGAATGCCGAAAAAAATTTTTTACTAAAAGT
2123












AAAAAATTTACCACGCGTGGCGGATGT


RB1
NM_000321
16
13
+
74
+
31
37
1
0
CGCGAATGCCGAAGTAAGTATTTTATAATCTTTT
2124












TTTTTTTCCTTACGCGTGGCGGATGT


RB1
NM_000321
16
13
+
105
−
0
37
1
0
CGCGAATGCCATGAAAAATGTTGTCATTCAGAA
2125












GTTTGCTAAAGGACGCGTGGCGGATGT


RB1
NM_000321
16
13
+
140
+
0
37
1
0
CGCGAATGCCATGTCTTTATTGGCGTGCGCTCTT
2126












GAGGTTGTAATACGCGTGGCGGATGT


RB1
NM_000321
16
13
+
175
−
0
37
1
0
CGCGAATGCCATTTATGAAAATTTAACTTACTGC
2127












TATATGTGGCCACGCGTGGCGGATGT


RPS6KA1
NM_002953
7
1
+
−16
+
0
37
1
0
CGCGAATGCCCTCCTGTCTTTTGCAGGTGATGTT
2128












CACGGAGGAGGACGCGTGGCGGATGT


RPS6KA1
NM_002953
7
1
+
19
−
0
37
1
0
CGCGAATGCCGCCCAGAGCCAGCTCAGCCAGGT
2129












AAAACTTCACATACGCGTGGCGGATGT


RPS6KA1
NM_002953
7
1
+
54
+
0
37
1
0
CGCGAATGCCCTGGATCACCTGCACAGCCTGGG
2130












TATCATTTACAGACGCGTGGCGGATGT


RPS6KA1
NM_002953
7
1
+
89
−
0
37
1
0
CGCGAATGCCCTGGAGGCTTCACTCACTTCTCA
2131












GGCTTGAGGTCTACGCGTGGCGGATGT


PKN1
NM_002741
3
19
+
0
+
0
37
1
0
CGCGAATGCCATGGCCCCCAGTCCCCTGGTGCG
2132












GGTGGCCCCACCACGCGTGGCGGATGT


PKN1
NM_002741
3
19
+
35
−
0
37
1
0
CGCGAATGCCAGGCCCGCCACGCGGCTCAGGTT
2133












GGTGGCCGAGCAACGCGTGGCGGATGT


PKN1
NM_002741
3
19
+
70
+
0
37
1
0
CGCGAATGCCGGAGAAGCAGTTGGCCATTGAGC
2134












TGAAGGTGAAGCACGCGTGGCGGATGT


PKN1
NM_002741
3
19
+
105
−
0
37
1
0
CGCGAATGCCATTGCTGTAGGTCTGGATCATGTT
2135












CTCCGCCCCCTACGCGTGGCGGATGT


PKN1
NM_002741
3
19
+
140
+
0
37
1
0
CGCGAATGCCGGCAGCACCAAGGTGAGGCAGC
2136












ACGTGCACACACAACGCGTGGCGGATGT


PDGFRA
NM_006206
16
4
+
0
+
0
37
1
0
CGCGAATGCCCTATGTTATTTTATCTTTTGAAAA
2137












CAATGGTGACTACGCGTGGCGGATGT


PDGFRA
NM_006206
16
4
+
35
−
0
37
1
0
CGCGAATGCCATACTGTGTAGTATCAGCCTGCTT
2138












CATGTCCATGTACGCGTGGCGGATGT


PDGFRA
NM_006206
16
4
+
70
+
0
37
1
0
CGCGAATGCCGTCCCCATGCTAGAAAGGAAAGA
2139












GGTTTCTAAATAACGCGTGGCGGATGT


PDGFRA
NM_006206
16
4
+
105
−
0
37
1
0
CGCGAATGCCCTGGACGATCATAGAGTGATCTC
2140












TGGATGTCGGAAACGCGTGGCGGATGT


PDGFRA
NM_006206
16
4
+
140
+
0
37
1
0
CGCGAATGCCCCTCATATAAGAAGAAATCTATG
2141












TTAGGTAAAAGTACGCGTGGCGGATGT


RB1
NM_000321
25
13
+
0
+
0
37
1
0
CGCGAATGCCACTTCTGAGAAGTTCCAGAAAAT
2142












AAATCAGATGGTACGCGTGGCGGATGT


RB1
NM_000321
25
13
+
35
−
0
37
1
0
CGCGAATGCCCAGCACTTCTTTTGAGCACACGG
2143












TCGCTGTTACATACGCGTGGCGGATGT


RB1
NM_000321
25
13
+
70
+
0
37
1
0
CGCGAATGCCAAGGAAGCAACCCTCCTAAACCA
2144












CTGAAAAAACTAACGCGTGGCGGATGT


RB1
NM_000321
25
13
+
105
−
0
37
1
0
CGCGAATGCCCCATCTGCTTCATCTGATCCTTCA
2145












ATATCAAAGCGACGCGTGGCGGATGT


RB1
NM_000321
25
13
+
140
+
0
37
1
0
CGCGAATGCCAAGGTAGGAACCAGTTTTGAATG
2146












TTTTCCAGTAGCACGCGTGGCGGATGT


PKN1
NM_213560
1
19
+
−50
+
0
37
1
0
CGCGAATGCCGGTGGGGCTGAGGTTCAGGAAGA
2147












GGGCGGGGCCCTACGCGTGGCGGATGT


PKN1
NM_213560
1
19
+
−15
−
0
37
1
0
CGCGAATGCCGGGTTATTGGCCTCCGCCATCCT
2148












GGGTCCGGGCTGACGCGTGGCGGATGT


PKN1
NM_213560
1
19
+
20
+
0
37
1
0
CGCGAATGCCCTCGGAGCAGGAGCTGGAGGTGG
2149












GGTCCAGGGTCCACGCGTGGCGGATGT


PKN1
NM_213560
1
19
+
55
−
0
37
1
0
CGCGAATGCCTGCCACTTGGGGCCCCCTTCTGCC
2150












TGCCCCCACAGACGCGTGGCGGATGT


PDGFRA
NM_006206
10
4
+
0
+
0
37
1
0
CGCGAATGCCATGTAATAATGAAACTTCCTGGA
2151












CTATTTTGGCCAACGCGTGGCGGATGT


PDGFRA
NM_006206
10
4
+
35
−
0
37
1
0
CGCGAATGCCGGAGTGGATCTCCGTGATGATGT
2152












TTGAGACATTGTACGCGTGGCGGATGT


PDGFRA
NM_006206
10
4
+
70
+
0
37
1
0
CGCGAATGCCCGAGACAGGAGTACCGTGGAGG
2153












GCCGTGTGACTTTACGCGTGGCGGATGT


PDGFRA
NM_006206
10
4
+
105
−
0
37
1
0
CGCGAATGCCGGCATCGCACGGCGATGGTCTCC
2154












TCCACTTTGGCGACGCGTGGCGGATGT


PDGFRA
NM_006206
10
4
+
140
+
0
37
1
0
CGCGAATGCCTGGCTAAGAATCTCCTTGGAGCT
2155












GAGAACCGAGAGACGCGTGGCGGATGT


PDGFRA
NM_006206
10
4
+
175
−
0
37
1
0
CGCGAATGCCCTGTTGAGGAACTCACTGGGAGC
2156












CACCAGCTTCAGACGCGTGGCGGATGT


NTRK3
NM_001012338
14
15
−
0
+
0
37
1
0
CGCGAATGCCGCAGTTGGAGCAGAACTTTTTCA
2157












ACTGCAGCTGTGACGCGTGGCGGATGT


NTRK3
NM_001012338
14
15
−
35
−
0
37
1
0
CGCGAATGCCCCCCTGCTCCTGCCAGAGCTGCA
2158












TCCAGCGGATGTACGCGTGGCGGATGT


NTRK3
NM_001012338
14
15
−
70
+
0
37
1
0
CGCGAATGCCGAGGCCAAGCTCAACAGCCAGA
2159












ACCTCTACTGCATACGCGTGGCGGATGT


NTRK3
NM_001012338
14
15
−
105
−
0
37
1
0
CGCGAATGCCTGCGGAAGAGAGGAAGCTGGGA
2160












GCCATCAGCGTTGACGCGTGGCGGATGT


NTRK3
NM_001012338
14
15
−
140
+
0
37
1
0
CGCGAATGCCTGAACATCAGTCAGTGTGGTGAG
2161












TGAGTGGCCGCCACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
0
+
0
37
1
0
CGCGAATGCCCCCGTCTGCCGTTTAAGCGCCTG
2162












AATCTTGTCCCAACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
35
−
0
37
1
0
CGCGAATGCCCCCTGATCGTCTGACATGTCATC
2163












GGCTTTCCCCTTACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
70
+
0
37
1
0
CGCGAATGCCTACTTCTGTGCAAAGTAAAAGCC
2164












CCGATTTAGAGGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
105
−
0
37
1
0
CGCGAATGCCCACATGACAGTTGTTTTCCAAGG
2165












TGTCCAAAGAGGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
140
+
0
37
1
0
CGCGAATGCCGGTTCTGACATAGACTTTAGACC
2166












GAAACTTGTCAAACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
175
−
0
37
1
0
CGCGAATGCCTATTTCTTAAAAAGTTATCTAAG
2167












GGACCCTTCCCGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
210
+
0
37
1
0
CGCGAATGCCGAATCGAAACCAGTATTGGCCAG
2168












AGCACAGTCATCACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
245
−
0
37
1
0
CGCGAATGCCTCTGGCTGCTCATTCGAGTCCTCT
2169












GTCAAATCAATACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
280
+
0
37
1
0
CGCGAATGCCCAGTCTTGTGGACCACAATAAAC
2170












TAAATTCTGAAGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
315
−
0
37
1
0
CGCGAATGCCTCGCTGGCCATTTATTGCCTCCCT
2171












GGAGGGAGAGGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
350
+
0
37
1
0
CGCGAATGCCGAAGACACTGGGGATCAGCAGG
2172












GGTTGTTGAAGGCACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
385
−
0
37
1
0
CGCGAATGCCTCTCTCCAGGAAATGCCAACTTG
2173












TCGTTCTGAATGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
420
+
0
37
1
0
CGCGAATGCCCCCTTTCAGACATTCCTTGCAAA
2174












ACAGAGGAGGAGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
455
−
0
37
1
0
CGCGAATGCCTCGCCTCTCCTCCCTGCACCTCCA
2175












CAGCCAACACCACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
490
+
0
37
1
0
CGCGAATGCCCTCCCAGGAATGTTCGCCACGGA
2176












GCTGCCCGGAGCACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
525
−
0
37
1
0
CGCGAATGCCCTCCTTTCTGGGGCACATTCTCGG
2177












GCCACTCGTCAACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
560
+
0
37
1
0
CGCGAATGCCCAGGACAGTTGGAGTGAAGCTGG
2178












GGGCATCCTGTTACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
595
−
0
37
1
0
CGCGAATGCCTGTCCTGCAAGACCACCATAGGC
2179












ACCTTCCCTTTGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
630
+
0
37
1
0
CGCGAATGCCTCTTGGCTGTGAGACCACCGCAA
2180












ATCAAGTCCCTTACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
665
−
0
37
1
0
CGCGAATGCCTCAGGGGTCATGTTCTTGCCTTGG
2181












GGTGTGGCTGGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
700
+
0
37
1
0
CGCGAATGCCGAGTGAGGTGCTGGAATCTTTCC
2182












CCGAAGAAGACTACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
735
−
0
37
1
0
CGCGAATGCCAGAGGGAGAGCTCAGGGACGAA
2183












TGGCTGAGTACAGACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
770
+
0
37
1
0
CGCGAATGCCTCCACCAGCTCGCCCGAGGGGCC
2184












GCCTGCTCCCCCACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
805
−
0
37
1
0
CGCGAATGCCAGGTGGGGAAGGGACTGGTACTG
2185












CTGTGCTGCTTTACGCGTGGCGGATGT


CHAF1A
NM_005483
3
19
+
840
+
0
37
1
0
CGCGAATGCCCCACGCCCCTCCGCAGAGTGAGT
2186












ATCTCCCATGGAACGCGTGGCGGATGT


EPHA4
NM_004438
18
2
−
−24
+
0
37
1
0
CGCGAATGCCGAGCAGCGTTGGCACCGGCGAAC
2187












CATGGCTGGGATACGCGTGGCGGATGT


EPHA4
NM_004438
18
2
−
11
−
0
37
1
0
CGCGAATGCCTCCCGAAGAGACACGAAAATAG
2188












GGCGAAATAGAAAACGCGTGGCGGATGT


EPHA4
NM_004438
18
2
−
46
+
0
37
1
0
CGCGAATGCCTTTGCGACGCTGTCACAGGTTCC
2189












AGGGTATACCCCACGCGTGGCGGATGT


EPHA4
NM_004438
18
2
−
81
−
0
37
1
0
CGCGAATGCCCACAGAAAGGCCGTCCCGCTCTT
2190












ACCTTCATTCGCACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
0
+
0
37
1
0
CGCGAATGCCTAATCATCATGTGTATGCATTCA
2191












GCTTTGATTGGGACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
35
−
0
37
1
0
CGCGAATGCCTGAGCACATTCCAAGAGATGCAT
2192












CTGAGTCTCTCCACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
70
+
0
37
1
0
CGCGAATGCCTGATCTGAAAATGACTGATGGAA
2193












CCTACGTCTTTGACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
105
−
0
37
1
0
CGCGAATGCCATAAGGTAAACTGTAGAGCAGGG
2194












CATCATAAGGAAACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
140
+
0
37
1
0
CGCGAATGCCAAGCACACCCCCTACCGGGTCCT
2195












AAGGAACAACCCACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
175
−
0
37
1
0
CGCGAATGCCTGGTCAACACTGCATCATAGGCT
2196












TCCCGGAGCTTTACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
210
+
0
37
1
0
CGCGAATGCCTTACAGTGGAGTCCCAAGAAAAG
2197












ACCTTCTATCAAACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
245
−
0
37
1
0
CGCGAATGCCGGAATTTCACCTCTTGCTGCTGCC
2198












TCTGTGAAGGCACGCGTGGCGGATGT


GUCY2F
NM_001522
18
X
−
280
+
0
37
1
0
CGCGAATGCCTGAGAAGCTGGAGTTCGATCAAG
2199












TAAGTACACATTACGCGTGGCGGATGT


CHAF1A
NM_005483
11
19
+
−24
+
0
37
1
0
CGCGAATGCCCTCTTTTTTTTTTCTCCTTTTGAGG
2200












ATGATGATGAACGCGTGGCGGATGT


CHAF1A
NM_005483
11
19
+
11
−
0
37
1
0
CGCGAATGCCAACCATCGTCCTCATCTTCATCCT
2201












CTCCCATGTCGACGCGTGGCGGATGT


CHAF1A
NM_005483
11
19
+
46
+
0
37
1
0
CGCGAATGCCTCTTTGTGCCCCATGGGTACCTGT
2202












CTGAGGACGAAACGCGTGGCGGATGT


CHAF1A
NM_005483
11
19
+
81
−
0
37
1
0
CGCGAATGCCGACCTCCCCCTTCACTCCCTCACC
2203












TCTGTCACACCACGCGTGGCGGATGT


RET
NM_020630
6
10
+
0
+
0
37
1
0
CGCGAATGCCGGCTGGTTCTCAACCGGAACCTC
2204












TCCATCTCGGAGACGCGTGGCGGATGT


RET
NM_020630
6
10
+
35
−
0
37
1
0
CGCGAATGCCTCATTGACCAGCACCGCCAGCTG
2205












CATGGTGCGGTTACGCGTGGCGGATGT


RET
NM_020630
6
10
+
70
+
0
37
1
0
CGCGAATGCCCTCAGACTTCCAGGGCCCAGGAG
2206












CGGGCGTCCTCTACGCGTGGCGGATGT


RET
NM_020630
6
10
+
105
−
0
37
1
0
CGCGAATGCCGCTGACCGGCAGCACCGACACGT
2207












TGAAGTGGAGCAACGCGTGGCGGATGT


RET
NM_020630
6
10
+
140
+
0
37
1
0
CGCGAATGCCCTGCACCTGCCCAGTACCTACTC
2208












CCTCTCCGTGAGACGCGTGGCGGATGT


RET
NM_020630
6
10
+
175
−
0
37
1
0
CGCGAATGCCATGGGCTCACCTGGGCAAATCGG
2209












CGAGCCCTCCTGACGCGTGGCGGATGT


NTRK3
NM_001012338
9
15
−
−38
+
0
37
1
0
CGCGAATGCCAGCTAACTGTCCTCCTCCTTTTTG
2210












TGTTTGGTTTTACGCGTGGCGGATGT


NTRK3
NM_001012338
9
15
−
−3
−
0
37
1
0
CGCGAATGCCAGTGATAGGAGGTGTGGGACTCA
2211












CTTCGTCAACTGACGCGTGGCGGATGT


NTRK3
NM_001012338
9
15
−
32
+
0
37
1
0
CGCGAATGCCGTGACCCACAAACCAGAAGAAG
2212












ACACTTTTGGGGTACGCGTGGCGGATGT


NTRK3
NM_001012338
9
15
−
67
−
0
37
1
0
CGCGAATGCCCAGTCAACACACTCCTCTTGACC
2213












AAGAAGTGACTCACGCGTGGCGGATGT


PDGFRA
NM_006206
5
4
+
−4
+
0
37
1
0
CGCGAATGCCATAGCAACATCAGAGCTGGATCT
2214












AGAAATGGAAGCACGCGTGGCGGATGT


PDGFRA
NM_006206
5
4
+
31
−
0
37
1
0
CGCGAATGCCCAATCGTTTCCCCTGACTTATACA
2215












CGGTTTTAAGAACGCGTGGCGGATGT


PDGFRA
NM_006206
5
4
+
66
+
0
37
1
0
CGCGAATGCCTGGTCACCTGTGCTGTTTTTAACA
2216












ATGAGGTGGTTACGCGTGGCGGATGT


PDGFRA
NM_006206
5
4
+
101
−
0
37
1
0
CGCGAATGCCCCTACCACTTCTCCAGGGTAAGT
2217












CCATTGAAGGTCACGCGTGGCGGATGT


RET
NM_020630
13
10
+
−16
+
0
37
1
0
CGCGAATGCCCTGTGCTGCATTTCAGAGAACGC
2218












CTCCCCGAGTGAACGCGTGGCGGATGT


RET
NM_020630
13
10
+
19
−
0
37
1
0
CGCGAATGCCTCAGGACGTTGAACTCTGACAGC
2219












AGGTCTCGCAGCACGCGTGGCGGATGT


RET
NM_020630
13
10
+
54
+
0
37
1
0
CGCGAATGCCAGCAGGTCAACCACCCACATGTC
2220












ATCAAATTGTATACGCGTGGCGGATGT


RET
NM_020630
13
10
+
89
−
0
37
1
0
CGCGAATGCCCTGCAGCTGGCCTTACCATCCTG
2221












GCTGCAGGCCCCACGCGTGGCGGATGT


PALB2
NM_024675
3
16
−
−26
+
0
37
1
0
CGCGAATGCCCAGCTTATTTATTTTTGTTATCTA
2222












AGGAATTTAAAACGCGTGGCGGATGT


PALB2
NM_024675
3
16
−
9
−
0
37
1
0
CGCGAATGCCCATCAATGTGCATCTTTTTCAGGA
2223












GTTGACCAGTTACGCGTGGCGGATGT


PALB2
NM_024675
3
16
−
44
+
0
37
1
0
CGCGAATGCCATTCTTACCAAGCTTCAGTCTGTC
2224












ACAAAGCCTATACGCGTGGCGGATGT


PALB2
NM_024675
3
16
−
79
−
0
37
1
0
CGCGAATGCCAGTGGTCCCAGCCAGTCATTACT
2225












TACCATTTCAGAACGCGTGGCGGATGT


EPHA4
NM_004438
5
2
−
0
+
0
37
1
0
CGCGAATGCCGGTGGCAAGATTCCTATCCGGTG
2226












GACTGCGCCAGAACGCGTGGCGGATGT


EPHA4
NM_004438
5
2
−
35
−
0
37
1
0
CGCGAATGCCCACTTGCTGATGTGAATTTACGA
2227












TAGGCAATTGCTACGCGTGGCGGATGT


EPHA4
NM_004438
5
2
−
70
+
0
37
1
0
CGCGAATGCCATGTATGGAGCTATGGAATCGTT
2228












ATGTGGGAAGTGACGCGTGGCGGATGT


EPHA4
NM_004438
5
2
−
105
−
0
37
1
0
CGCGAATGCCGACATATCCCAATAGGGCCTCTC
2229












CCCGTACGACATACGCGTGGCGGATGT


EPHA4
NM_004438
5
2
−
140
+
0
37
1
0
CGCGAATGCCCAATCAAGATGTAAGTCTCTATG
2230












TTCTGAAATATAACGCGTGGCGGATGT


NTRK3
NM_001012338
13
15
−
0
+
0
37
1
0
CGCGAATGCCACCTTCCTGAGATCAGCGTGAGC
2231












CACGTCAACCTGACGCGTGGCGGATGT


NTRK3
NM_001012338
13
15
−
35
−
0
37
1
0
CGCGAATGCCCAAGTGATAACAGCGTTGTCACC
2232












CTCTCGTACGGTACGCGTGGCGGATGT


NTRK3
NM_001012338
13
15
−
70
+
0
37
1
0
CGCGAATGCCCAATGGCTCTGGATCACCCCTTC
2233












CTGATGTGGACTACGCGTGGCGGATGT


NTRK3
NM_001012338
13
15
−
105
−
0
37
1
0
CGCGAATGCCGTGAGTGTTGATGGACTGCAGCC
2234












CAGTGACTATCCACGCGTGGCGGATGT


NTRK3
NM_001012338
13
15
−
140
+
0
37
1
0
CGCGAATGCCCAGGTAGGCATCCTGGGCTTCAG
2235












CCCCATCAGGAGACGCGTGGCGGATGT


RET
NM_020630
4
10
+
0
+
0
37
1
0
CGCGAATGCCGTGAGGGTCTGCCCTTCCGCTGC
2236












GCCCCGGACAGCACGCGTGGCGGATGT


RET
NM_020630
4
10
+
35
−
0
37
1
0
CGCGAATGCCTCGCGGTCCAGGGCCCAGCGCGT
2237












GCTCACCTCCAGACGCGTGGCGGATGT


RET
NM_020630
4
10
+
70
+
0
37
1
0
CGCGAATGCCGCAGCGGGAGAAGTACGAGCTG
2238












GTGGCCGTGTGCAACGCGTGGCGGATGT


RET
NM_020630
4
10
+
105
−
0
37
1
0
CGCGAATGCCCATCACCACCTCCTCGCGCGCGC
2239












CGGCGTGCACGGACGCGTGGCGGATGT


RET
NM_020630
4
10
+
140
+
0
37
1
0
CGCGAATGCCGTGCCCTTCCCGGTGACCGTGTA
2240












CGACGAGGACGAACGCGTGGCGGATGT


RET
NM_020630
4
10
+
175
−
0
37
1
0
CGCGAATGCCCGGTGTCGACGCCCGCGGGGAAG
2241












GTGGGCGCCGAGACGCGTGGCGGATGT


RET
NM_020630
4
10
+
210
+
0
37
1
0
CGCGAATGCCCCAGCGCCGTGGTGGAGTTCAAG
2242












CGGAAGGAGGTGACGCGTGGCGGATGT


GUCY2F
NM_001522
7
X
−
0
+
0
37
1
0
CGCGAATGCCATCAGTTGCTGAATCTCTCAAAA
2243












AGGGCTGCACAGACGCGTGGCGGATGT


GUCY2F
NM_001522
7
X
−
35
−
0
37
1
0
CGCGAATGCCGTACAAGGTGACCAAGTCAAAGC
2244












CCTCAGGTTCAAACGCGTGGCGGATGT


GUCY2F
NM_001522
7
X
−
70
+
0
37
1
0
CGCGAATGCCTTCAGCGACATTGTGGGCTTCAC
2245












AACCATTTCAGCACGCGTGGCGGATGT


GUCY2F
NM_001522
7
X
−
105
−
0
37
1
0
CGCGAATGCCTCAGAAGATCCACGACCTCAATG
2246












GGCTCACTCATGACGCGTGGCGGATGT


GUCY2F
NM_001522
7
X
−
140
+
0
37
1
0
CGCGAATGCCATGACCTGTACACACTCTTTGAT
2247












GCAATAATTGGCACGCGTGGCGGATGT


GUCY2F
NM_001522
7
X
−
175
−
0
37
1
0
CGCGAATGCCCCTGCTAATTAACCTACCTTGTAG
2248












ACATCATGACTACGCGTGGCGGATGT


NTRK3
NM_001012338
5
15
−
0
+
0
37
1
0
CGCGAATGCCGCCCTGAAGGATCCCACCCTGGC
2249












TGCCCGGAAGGAACGCGTGGCGGATGT


NTRK3
NM_001012338
5
15
−
35
−
0
37
1
0
CGCGAATGCCGCAGGTTGGTGAGCAGCTCGGCC
2250












TCCCTCTGGAAAACGCGTGGCGGATGT


NTRK3
NM_001012338
5
15
−
70
+
0
37
1
0
CGCGAATGCCAGCATGAGCACATTGTCAAGTTC
2251












TATGGAGTGTGCACGCGTGGCGGATGT


NTRK3
NM_001012338
5
15
−
105
−
0
37
1
0
CGCGAATGCCTATTCAAAGACCATGATGAGGGG
2252












GTCCCCATCGCCACGCGTGGCGGATGT


NTRK3
NM_001012338
5
15
−
140
+
0
37
1
0
CGCGAATGCCCATGAAGCATGGAGACCTGAATA
2253












AGTTCCTCAGGTACGCGTGGCGGATGT














































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































While the preferred embodiment of the invention has been illustrated and described, it will be appreciated that various changes can be made therein without departing from the spirit and scope of the invention.