(en)Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having β-ketoacyl-acp synthase activity.

1.ApplicationNumber: US-201314650953-A
1.PublishNumber: US-9512447-B2
2.Date Publish: 20161206
3.Inventor: SHANKAR SMITA
CLARK LOUIS
OSBORNE ROBERT
VROOM JONATHAN
VALLE FERNANDO
CHO CATHERINE M.

4.Inventor Harmonized: SHANKAR SMITA(US)
CLARK LOUIS(US)
OSBORNE ROBERT(US)
VROOM JONATHAN(US)
VALLE FERNANDO(US)
CHO CATHERINE M(US)

5.Country: US
6.Claims:
(en)Genetically engineered cells and microorganisms are provided that produce fatty alcohols and fatty acids. In particular, engineered microbial cells comprise a modified native gene having β-ketoacyl-acp synthase activity.

7.Description:
(en)The present application is a national stage application filed under 35 USC §371, and claims priority to PCT/US2013/072973, filed Dec. 4, 2013, which claims priority to U.S. Prov. Appln. Ser. No. 61/737,232, filed Dec. 14, 2012, each of which is hereby incorporated by reference in its entirety for all purposes.


REFERENCE TO A “SEQUENCE LISTING,” A TABLE, OR A COMPUTER PROGRAM LISTING APPENDIX SUBMITTED AS AN ASCII FILE
The official copy of the Sequence Listing is submitted as an ASCII formatted text file via EFS-Web, with a file name of “CX5-111WO1_ST25_substitute.txt”, a creation date Sep. 16, 2015, and a size of 467 kilobytes. The Sequence Listing filed via EFS-Web is part of the specification and is incorporated in its entirety by reference herein.
FIELD OF THE INVENTION
The present disclosure relates to recombinant microorganisms and particularly to recombinant bacterial microorganisms exhibiting production of C12 to C14 fatty alcohol compositions.
BACKGROUND
Crude petroleum has traditionally been used as a primary source for raw materials for producing numerous specialty chemicals. Particular specialty chemicals that can be produced from the petrochemical raw materials include fatty alcohols. Fatty alcohols have many industrial and commercial uses. For example, fatty alcohols act as surfactants which are useful in personal care and household products, such as detergents. Fatty alcohols are also used in waxes, lubricating oils, cosmetics and solvents. However, obtaining fatty alcohols from crude petroleum requires a significant amount of energy and involves the use of a non-renewable energy source.
Further, even those fatty alcohols that are obtained from renewable sources, such as from plant or animal derived fatty acids, generally are prepared using a hydrogenation step. Hydrogenation is a costly process step but is utilized to eliminate the double bonds of unsaturated fatty acids. A number of prior art references disclose genetically engineered microorganisms that produce products including fatty acid derivatives such as fatty acid esters and fatty alcohols (See e.g., WO2007/136762, WO2008/119082, WO2010/075483, WO2011/008535, WO2011/019858, U.S. Pat. Nos. 6,143,538, and 8,110,670, and U.S. Pat. Appln. Publ. Nos. 2012/0115195 and 2012/0142979. However, a need still exists in the field for improved fatty alcohol production from bioengineered microorganisms that is efficient and cost effective and further that is tailored for use in particular industrial applications.
Regulation of fatty acid metabolism to achieve higher yields of fatty acids and/or to maximize yields of fatty acids with specific carbon chain lengths would also be desirable for producing downstream products including but not limited to fatty alcohols, which additionally may be used as components of or precursors to compounds used as soaps, polymer additives, surfactants, and the like.
SUMMARY
The biological production of fatty alcohols in engineered microbial organisms according to the instant invention requires the activity of a fatty alcohol forming reductase (FAR) and the modification of a native gene having β-ketoacyl-ACP synthase activity. The FAR catalyzes the reduction of fatty acid acyl-ACP substrates and/or fatty acid acyl-CoA substrates to produce fatty alcohols. Fatty acids which are used as FAR substrates may be synthesized by different enzyme complexes depending on the host organism. Certain organisms synthesize fatty acids by a fatty acid synthesis I (“FAS-I”) scheme, where the enzymatic activities are present in a large multifunctional protein. Other organisms synthesize fatty acids by a fatty acid synthesis II (“FAS-II”) scheme, where each biosynthetic step is catalyzed by a mono-functional protein. FAS-I is typical of yeast and animal cells. FAS-II is typically found in bacteria. Reference is made to FIG. 1 which shows the reaction schemes for the production of fatty alcohols by the activity of FAR which converts fatty acid substrates produced during fatty acid biosynthesis (“fab”) in an E. coli FAS-II system.
In general, during biosynthesis of fatty acids the fatty acid chain is extended by the addition of two carbons at each elongation step. The elongation reaction step is carried out by β-ketoacyl-ACP synthases (KAS). KAS catalyzes the reaction of acyl-CoA or acyl-ACP with malonyl-CoA or malonyl-ACP [Chen Y., et al., 2011. Prot. Sci. 20: 1659-1667] to produce fatty acids with varying chain lengths. E. coli has 3 KAS enzymes: KASI (EC 2.3.1.41) coded by the fabB gene which catalyzes the reaction of acyl-ACP with malonyl-ACP to form 3-oxoacyl-ACP, KASII (EC 2.3.1.79) coded by the fabF gene which catalyzes the final elongation to C18:0, and KASIII (EC 2.3.1.180) coded by the fabH gene which catalyzes the initial condensation of malonyl-ACP with acetyl-CoA.
Generally, the FabB enzyme is the only enzyme capable of elongating the cis-3-decenoyl-ACP to form unsaturated C16:1 and C18:1 fatty acids. By modifying the native gene (e.g., by providing a variant FabB enzyme, which in some embodiments replaces the native fabB gene in a host cell) a higher percent of C12 to C14 fatty acids and fatty alcohols are produced as compared to an engineered host cell comprising the native gene encoding FabB or FabF.
In one aspect, the invention relates to a method of altering the carbon chain length of acyl-derived products produced from a bacterial microorganism, which comprise modifying a native bacterial gene having β-ketoacyl-ACP synthase activity. In certain embodiments the native bacterial gene is a native β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) and in certain embodiments the native bacterial gene having β-ketoacyl-ACP synthase activity is a native β-ketoacyl-ACP synthase II (FabF) (EC 2.3.1.179).
In another aspect, the invention relates to variant β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) polypeptides wherein the variant comprising at least about 85% amino acid sequence identity to SEQ ID NO:2 or a functional fragment thereof and comprising an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2. In another aspect, the invention relates to variant β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) polypeptides wherein the variant comprising at least 85% amino acid sequence identity to SEQ ID NO:2 or a functional fragment thereof and comprising an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2. In another aspect, the invention relates to variant β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) polypeptides wherein the variant comprising at least 85%, at least 86%, at least 87%, at least 88%, at least 89%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% amino acid sequence identity to SEQ ID NO:2 or a functional fragment thereof and comprising an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2. In another aspect, the invention relates to variant β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) polypeptides wherein the variant comprising at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or about 100% amino acid sequence identity to SEQ ID NO:2 or a functional fragment thereof and comprising an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2.
In certain embodiments, the invention relates to a polynucleotide encoding a variant as described above. The polynucleotide may be a codon optimized polynucleotide sequence. In some embodiments of this aspect, the invention relates to an engineered host cell which incorporates a polynucleotide encoding a FabB variant. In other embodiments, the engineered host cell having a polynucleotide encoding a variant FabB further comprises a heterologous polynucleotide encoding a fatty alcohol reductase (FAR) enzyme (e.g., a FAR having at least about 80% amino acid sequence identity to SEQ ID NO:4 or SEQ ID NO:6 and optionally comprises one or more heterologous polynucleotides encoding FabI, FabZ, FabH or FabD and/or a heterologous polynucleotide encoding a thioesterase. In other embodiments, the engineered host cell having a polynucleotide encoding a variant FabB further comprises a heterologous polynucleotide encoding a fatty alcohol reductase (FAR) enzyme (e.g., a FAR having at least 80% amino acid sequence identity to SEQ ID NO:4 or SEQ ID NO:6 and optionally comprises one or more heterologous polynucleotides encoding FabI, FabZ, FabH or FabD and/or a heterologous polynucleotide encoding a thioesterase. In additional embodiments, the engineered microorganism is capable of producing fatty alcohol compositions comprising at least about 25% (e.g., at least about 30%, 35%, 40%, 45%, or 50%) of C12 to C14 fatty alcohols. In additional embodiments, the engineered microorganism is capable of producing fatty alcohol compositions comprising at least 25% (e.g., at least 30%, 35%, 40%, 45%, or 50%) of C12 to C14 fatty alcohols.
In further aspects, the invention relates to a recombinant cell culture comprising engineered bacterial cells, said engineered bacterial cells comprising (a) a modified native gene having β-ketoacyl-ACP synthase activity and (b) one or more heterologous polynucleotides encoding a fatty alcohol reductase (e.g., EC1.1.1.*) and/or a thioesterase (EC3.1.2.- or EC 3.1.1.5). In some embodiments, the modification of the native gene results in the production of a protein having a substitution of one or more amino acid residues as compared to the protein produced by the expression of the native gene. In some embodiments, the engineered bacterial cells comprise a polynucleotide encoding a heterologous fatty alcohol reductase comprising at least 80%, sequence identity to SEQ ID NOS:4 or 6. In further embodiments the engineered cells produce a fatty alcohol composition selected from a) at least 60% C12 to C16 fatty alcohols relative to the total fatty alcohols produced by the engineered microbial cells; b) at least 25% C12 to C14 fatty alcohols relative to the total fatty alcohols produced by the engineered microbial cells; c) at least 10% C12 fatty alcohols relative to the total fatty alcohols produced by the engineered microbial cells; d) at least 20% C14 fatty alcohols relative to the total fatty alcohols produced by the engineered microbial cells; and/or e) less than 5% C18 fatty alcohols relative to the total fatty alcohols produced by the engineered cells. In other embodiments of this aspect, the produced fatty alcohols are secreted from the engineered microbial cells and are found in the culture medium.
In yet another aspect, the invention relates to methods of producing fatty alcohol compositions comprising culturing an engineered cell of the invention with a carbon substrate under suitable culture conditions to allow expression of a variant FabB and a heterologous FAR and further allowing production of fatty alcohols, wherein the produced fatty alcohol composition comprises at least 20% C12 and C14 fatty alcohols. In some embodiments of this method the fatty alcohol composition is recovered from the engineered cells and/or from the culture medium. In certain embodiments at least 1.0 g/L of fatty alcohols are produced. In certain embodiments a) at least 80% of the produced fatty alcohols have a carbon chain length of C10 to C16; b) at least 60% of the produced fatty alcohols have a carbon chain length of C12 to C16; and/or c) at least 30% of the produced fatty alcohols have a carbon chain length of C12 to C14. In certain embodiments, the carbon substrate is a fermentable sugar obtained from cellulosic feedstock. In certain embodiments the cellulosic feedstock is derived from wheat, wheat straw, sorghum, rice, barley, corn grain, corn cobs, corn stover, sugar cane straw, sugar cane bagasse, switchgrass or a combination thereof.
In yet additional aspects the invention relates to the use of the fatty alcohol compositions produced according to the methods outlined in the disclosure wherein the fatty alcohols composition is used in a cleaning composition, such as a component of a detergent composition or a hard surface cleaning composition and in a personal care compositions.
Other feature and advantages of the invention will be apparent from the following detailed description and from the claims.



BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 schematically depicts a pathway for the production of fatty alcohols and fatty acids by an embodiment of the invention.



DESCRIPTION OF THE INVENTION
The technical and scientific terms used in the descriptions herein will have the meanings commonly understood by one of ordinary skill in the art, unless specifically defined otherwise. Accordingly, the following terms are intended to have the following meanings.
Also, as used herein, the singular “a”, “an,” and “the” include the plural references, unless the context clearly indicates otherwise. Further, the term “or” is used in the present application to mean the disjunctive “or” and the conjunctive “and”.
Amino acids are designated using the three-letter symbols or one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Unless otherwise indicated, nucleic acids are written left to right in 5′ to 3′ orientation; amino acid sequences are written left to right in amino to carboxy orientation, respectively.
“EC” number refers to the Enzyme Nomenclature of the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB). The IUBMB biochemical classification is a numerical classification system for enzymes based on the chemical reactions they catalyze.
Numeric ranges are inclusive of the numbers defining the range. Thus, every numerical range disclosed herein is intended to encompass every narrower numerical range that falls within such broader numerical range, as if such narrower numerical ranges were all expressly written herein. It is also intended that every maximum (or minimum) numerical limitation disclosed herein includes every lower (or higher) numerical limitation, as if such lower (or higher) numerical limitations were expressly written herein.
Furthermore, the headings provided herein are not limitations of the various aspects or embodiments of the invention which can be had by reference to the application as a whole. Accordingly, the terms defined immediately below are more fully defined by reference to the application as a whole. Nonetheless, in order to facilitate understanding of the invention, a number of terms are defined below.
As used herein, the term “comprising” and its cognates are used in their inclusive sense (i.e., equivalent to the term “including” and its corresponding cognates).
The term “fatty alcohol” as used herein refers to an alcohol having the formula R—OH, where the R group is at least 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 or more carbons in length. R can be saturated or unsaturated. Further saturated or unsaturated fatty alcohols can be described as “Ca:b-OH”, wherein “a” is an integer that represents the total number of carbon atoms in the fatty alcohol and “b” is an integer that refers to the number of double bonds in the carbon chain. In some embodiments the R group of the fatty alcohol is a straight chain and in other embodiments the R group of the fatty alcohol is branched. In some embodiments, a fatty alcohol produced according to the methods disclosed herein is a C8-C20 saturated or unsaturated fatty alcohol (i.e., a C8, C9, C10, C11, C12, C13, C14, C15, C16, C17, C18, C19, or C20 fatty alcohol). In some embodiments, multiple fatty alcohols are produced with varying saturation levels. For example, in some embodiments, C10, C12, C14, and/or C16 fatty alcohols are produced. It is understood that a reference to a “Cx fatty alcohol” (e.g., C12) includes both saturated and unsaturated fatty alcohols having “x” carbon atoms unless indicated otherwise. In some embodiments, the fatty alcohol is any alcohol made from a fatty acid.
Fatty alcohol derivatives as used herein means compounds such as but not limited to fatty alcohols, fatty acid esters (e.g. acetyl, methyl or ethyl esters and waxes) and fatty acids. Fatty alcohol derivatives includes compounds derived from the fatty acid biosynthetic pathway as well as compounds derived from the fatty alcohols produced by the engineered microbial cells encompassed by the invention. In one example, the carbon chain length of any fatty alcohol derivative compound may be the same as the carbon chain length of the fatty alcohol from which is was derived.
The term “carbon chain length” as used herein means the number of carbon atoms in a carbon chain of a fatty alcohol, fatty alcohol substrate (e.g., a fatty acid) or fatty alcohol derivative. For example, the term “C12 fatty alcohol” refers to a fatty alcohol molecule having 12 carbons.
The term a “fatty alcohol composition” as used herein, means a composition which encompasses at least one fatty alcohol and which is produced from an engineered microbial organism according to the methods of the invention. The fatty alcohol compositions of the invention may include one or more fatty alcohols. For example a fatty alcohol composition may include only C12 fatty alcohols or a fatty alcohol composition may include a combination of C12, C14 and C16 fatty alcohols and these fatty alcohols may be saturated or unsaturated fatty alcohols and further may be straight chains or branched.
The term “fatty acid” as used herein means a compound having the formula RCO 2 H, wherein R is at least two carbons in length and generally between 4 and 22 carbons in length. Fatty acids may be saturated or unsaturated and further “R” can be straight or branched.
The term “acyl-ACP as used herein means a compound having the formula RCO-S-ACP, wherein “R” is at least three carbons in length and may be a straight chain or branched chain and further saturated or unsaturated. The abbreviation “ACP” refers to the acyl carrier protein that comprises a 4-phosphopantethiene moiety which can form a thioester linkage with the growing fatty acid chain during the biosynthesis of fatty acids.
The term “acyl-derived product” refers to any product of the fatty acid biosynthetic pathway obtained by the action of one or more enzymes which use acyl-ACPs as a substrate. (For example, the following enzymes use acyl-ACPs as substrates to produce fatty acids, fatty alcohols and/or fatty aldehydes: (1) fatty acid reductases (FARs); (2) ACP-thioesterases and (3) acyl-ACP reductases (AARs).
The terms “fatty acyl-CoA reductase”, “fatty acyl reductase”, and “fatty acyl acyl-ACP reductase” (EC 1.1.1.*) (collectively and individually referred to herein as FAR) are used interchangeably to refer to an enzyme that catalyzes the reduction of a fatty acyl-CoA, a fatty acyl-ACP, or other fatty acyl thioester. FAR enzymes can be divided into two general groups that differ with respect to the end-product synthesized. In one group the product that is formed by the action of the enzyme is an aldehyde. In the second group the product that is formed by the action of the enzyme is an alcohol. (Reference is made to Rowland et al., Plant Sci. 193-194 (2012) 28-38).
The term “acyl-CoA” refers to an acyl thioester formed between the carbonyl carbon of an alkyl chain and the sulfhydryl group of the 4′-phosphopantetthionyl moiety of co-enzyme A (CoA) which has the formula R—C(O)—S-CoA, wherein R is an alkyl group having at least 4 carbon atoms and preferably between 10 and 14 carbon atoms. R may be straight or branched and saturated or unsaturated. CoA refers to coenzyme A, the naturally occurring thiol compound having CAS number 85-61-0.
The phrase “fatty acid biosynthetic (“Fab”) enzymes” as used herein means a complex of enzymes involved in a number of reactions to produce saturated and unsaturated fatty acids. The process is primed by the enzymatic conversion of malonyl-CoA into malonyl-ACP and continues by successive addition of 2 carbons derived from malonyl-ACP residues, providing ACP intermediates (i.e., acyl-ACPs). There are at least 8 enzymes involved fatty acid initiation and elongation biosynthesis including FabA, FabB, FabD, FabF, FabG, FabH, FabI, and FabZ, collectively and individually referred to herein as “fatty acid biosynthetic” enzymes. Furthermore, the ACP protein plays a key role in fatty acid biosynthesis by anchoring the nascent acyl chain and making the acyl chain accessible to other enzymes (See, FIG. 1 ).
The term “FabD” refers to a malonyl-CoA-ACP transferase (EC 2.3.1.39).
The term “FabF” refers to a β-ketoacyl-ACP synthase II (3-oxoacyl-ACP synthetase (EC 2.3.1.179) which catalyzes the conversion of palmitoleate to cis-vaccenate.
The term “FabG” refers to a 3-ketoacyl-ACP-reducatse (3-oxoacyl ACP reductase) (EC 1.1.1.100) which catalyzes the NADPH dependent reduction of beta-ketoacyl-ACP substrates to beta-hydroxyacyl-ACP products, the first reductive step in the elongation cycle of fatty acid biosynthesis.
The term “FabI” refers to a trans-2-enoyl-ACP reductase (EC 1.3.1.9 and 1.3.1.10) that catalyzes the reaction of a trans-2,3-dehydroacyl-[ACP]+NAD(P)H+H + to an acyl-ACP+NAD(P) + .
The term “FabZ” refers to a beta-hydroxyacyl-ACP dehydratase (EC 4.2.1.59 to 4.2.61) that catalyzes the reaction of a (3R)-3-hydroxyacyl-ACP to a transΔ 2 -enoylacylACP+H 2 O.
The term “FabB” refers to a beta-ketoacyl-ACP synthase I (EC 2.3.1.41) that catalyzes the chemical reaction of an acyl-ACP to a 3-oxoacyl-ACP.
The term “FabH” refers to 3-oxoacyl-(acyl-carrier protein) synthase III activity and is used interchangeably with “KASIII” and “β-ketoacyl-ACP synthase III.” FabH catalyzes the initial condensation reaction in the fatty acid biosynthetic pathway using an acyl-CoA as a primer and is categorized as EC 2.3.1.180. The FabH enzymes have a His-Asn-Cys catalytic triad at their active site.
The term “FadD” refers to an “acyl-CoA synthetase (“ACS”) (EC 6.2.1 (acid-thiol ligases)). In some embodiments, the ACS is classified as EC 6.2.1.3. These ACSs are also known as long chain fatty acid-CoA ligases. An ACS catalyzes the reaction of free fatty acids (both saturated and unsaturated fatty acids) into metabolically active CoA esters (e.g., acyl-CoA) during fatty acid degradation. In some embodiments the FadD may be classified as EC 2.3.1.86 (fatty acyl CoA synthase).
The term “FadR” protein as used herein, refers to a multifunctional dual regulator that exerts negative control over the fatty acid degradative regulon and activates expression of fabA and fabF. The FadR regulator is encoded by a fadR gene. A “regulon” comprises a set of genes under control of a single regulatory protein.
The term “FadE” enzyme as used herein means an acyl-CoA dehydrogenase enzyme (EC 1.3.99.-). A FadE gene is also known as yafH.
Throughout the specification a reference may be made using an abbreviated gene name or an enzyme name. For example “fabB” refers to a gene encoding a β ketoacyl ACP synthase I, KAS-I or as sometimes referred to herein a FabB enzyme.
The term “thioesterase or thioester hydrolase (TE)” enzyme used herein means an enzyme having thioesterase activity. TEs are identified as members of EC 3.1.2.1 to EC 3.1.2.27 and also EC3.1.1.5 and EC 3.1.2.- and these enzyme which hydrolyze the thioester bond between a carbonyl group and a sulfur atom are classified based on enzyme function and substrate identity. In addition, TEs are classified based on the ThYme database (Thioester-active enzyme). In this classification system, TEs have been classified based on amino acid sequence similarity. Under the ThYme system, TEs are further divided into 24 different families (TE1-TE24). Reference is made to D. C. Cantu et al., (2010) Protein Science, 19:1281-1295 and D. C. Cantu et al., (2011) Nucleic Acid Research 39:doi10:1093/nar/gkq1072. TEs have the ability to catalyze a thioester cleavage reaction hydrolyzing a thioester into an acid and a thiol. TEs useful in the invention may be obtained from any suitable sources, including but not limited to plant, bacterial, algal, and fungal sources.
The term “analogous sequence” or “homologous sequence” as used herein means a sequence wherein the function of the gene is essentially the same as a reference gene. For example, a reference gene may be a fabB gene from E. coli . In some embodiments, the analogous sequence will have at least about 60%, for example, at least about 65%, 70%, 75%, 80%, 85%, 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% sequence identity with the reference sequence.
The term “wild-type” or “native” as used herein refers to the form found in nature. For example a naturally occurring or wild-type polypeptide or polynucleotide sequence is a sequence present in an organism that can be isolated from a source in nature and which has not been intentionally modified by human manipulation. When used in reference to a microorganism, the term means a naturally occurring (not genetically modified or engineered) microorganism.
The term “substrate” as used herein refers to a substance or compound that is converted or suitable for conversion into another compound (e.g., a product) by the action of at least one enzyme. The term includes not only a single compound but also combinations comprising more than one compound.
The term “conversion” as used herein refers to the enzymatic transformation of a substrate to at least one corresponding product. “Percent conversion” refers to the percent of the substrate that is converted to the product(s) within a specified period of time and under specified conditions.
Nucleic acid sequences may be “introduced” into a cell by protoplast fusion, transfection, transduction, transformation, electroporation or any other suitable method known in the art. A nucleic acid sequence introduced into a prokaryotic cell may be integrated into a chromosome or may be maintained as an episome.
The terms “transformed” and “stably transformed” as used herein refer to a cell that has a non-native (i.e., heterologous) polynucleotide sequence integrated into its genome or as an episomal plasmid that is maintained for multiple (e.g., at least two) generations.
The term “gene” as used herein refers to a polynucleotide (e.g., a DNA segment), that encodes a polypeptide and includes regions preceding and following the coding regions as well as intervening sequences (introns) between individual coding segments (exons).
The terms “endogenous” when used in reference to a gene refers to a gene that is found in a parental strain of a cell (e.g., a bacterial cell). As used herein in making comparisons between endogenous nucleic acid sequences, “homologous genes” (or “homologue” genes) refers to genes from different, but usually related species, which correspond to each other and are identical or very similar to each other. The term encompasses genes that are separated by speciation (i.e., the development of new species) (e.g., orthologous genes), as well as genes that have been separated by genetic duplication (e.g., paralogous genes).
The term “heterologous” polynucleotide as used herein means any polynucleotide that is introduced into a host cell by laboratory techniques, and includes polynucleotides that are removed from a host cell, subjected to laboratory manipulation, and then reintroduced into a host cell.
In some embodiments, when “heterologous” is used with reference to a nucleic acid or polypeptide, the term refers to a sequence that is not normally expressed and secreted by an organism (e.g., a “wild-type” organism). In some embodiments, the term encompasses a sequence that comprises two or more subsequences which are not found in the same relationship to each other as normally found in nature, or is recombinantly engineered so that its level of expression, or physical relationship to other nucleic acids or other molecules in a cell, or structure, is not normally found in nature. For instance, a heterologous nucleic acid is typically recombinantly produced, having two or more sequences from unrelated genes arranged in a manner not found in nature (e.g., a nucleic acid open reading frame (ORF) of the invention operatively linked to a promoter sequence inserted into an expression cassette, such as a vector).
As used herein, a “heterologous enzyme” is used in reference to an enzyme that is encoded by a heterologous gene. However, it is also contemplated herein that a heterologous gene can encode an endogenous or homologous enzyme. As used herein, the term “heterologous gene” refers to a gene that occurs in a form not found in a parental strain of the host cell. Thus, in some embodiments, a heterologous gene is a gene that is derived from a species that is different from the species of the host cell expressing the gene. In some embodiments, a heterologous gene is a modified version of a gene that is endogenous to the host cell (e.g., an endogenous gene subjected to manipulation and then introduced or transformed into the host cell). For example, in some embodiments, a heterologous gene has an endogenous coding sequence, but has modifications in the promoter sequence. Similarly, in other embodiments, a heterologous gene encodes the same amino acid sequence as an endogenous gene, but has modifications in codon usage and/or to noncoding regions (e.g., introns), and/or combinations thereof. In some embodiments, the heterologous gene is a gene that has been modified to overexpress a gene product of interest.
The term “expression” as used herein includes any step involved in the production of a polypeptide (e.g., encoded enzyme) including, but not limited to, transcription, post-transcriptional modification, translation, post-translational modification, and secretion.
The term “overexpression” as used herein refers to any state in which a gene is caused to be expressed at an elevated rate or level as compared to the endogenous (native) expression rate or level for that gene. In some embodiments, “overexpression” includes an elevated translation rate or level of the gene compared to the endogenous translation rate or level for that gene. In some embodiments, overexpression includes an elevated transcription rate or level of the gene compared to the endogenous transcription rate or level for that gene. It is intended that the term encompass overexpression of endogenous, as well as heterologous proteins.
The term “recombinant” as used herein includes reference to a cell or vector, that has been modified by the introduction of a heterologous nucleic acid sequence or that the cell is derived from a cell so modified. Thus, for example, recombinant cells express genes that are not found in identical form within the native (i.e., non-recombinant) form of the cell or express native genes that are otherwise abnormally expressed, under-expressed or not expressed at all as a result of deliberate human intervention. “Recombinant,” “engineered,” and “non-naturally occurring,” when used with reference to a cell, nucleic acid, or polypeptide, refers to a material, or a material corresponding to the natural or native form of the material, that has been modified in a manner that would not otherwise exist in nature, or is identical thereto but produced or derived from synthetic materials and/or by manipulation using recombinant techniques. Non-limiting examples include, among others, recombinant cells expressing genes that are not found within the native (i.e., non-recombinant) form of the cell or express native genes that are otherwise expressed at a different level.
The term “plasmid” as used herein refers to a circular double-stranded (ds) DNA construct used as a cloning vector, and which forms an extrachromosomal self-replicating genetic element in some eukaryotes or prokaryotes, or integrates into the host chromosome.
The term “operably linked” as used herein refers to a configuration in which a control sequence is appropriately placed (i.e., in a functional relationship) at a position relative to a polynucleotide of interest such that the control sequence directs or regulates the expression of the polynucleotide and/or polypeptide of interest. Thus, a nucleic acid is “operably linked” to another nucleic acid sequence when it is placed into a functional relationship with another nucleic acid sequence.
The term “control sequence” as used herein includes all components, that are necessary and/or advantageous for the expression of a polynucleotide of the present disclosure. Each control sequence may be native or foreign to the polynucleotide of interest. Such control sequences include, but are not limited to, leaders, polyadenylation sequences, propeptide sequences, promoters, signal peptide sequences, and transcription terminators.
“Host cell” as used herein refers to a living cell or microorganism that is capable of reproducing its genetic material and along with it recombinant genetic material that has been introduced into it (e.g., via heterologous transformation).
The terms “engineered host cell” or “recombinant host cell” used interchangeably herein refer to a cell whose genetic material has been altered using genetic engineering techniques. An engineered host cell also refers to a derivative of or the progeny of a cell whose genetic material has been altered using genetic engineering techniques. An example of a genetic modification as a result of genetic engineering techniques includes a modification to the genomic DNA. Another example of a genetic modification as a result of genetic engineering techniques includes introduction of a stable heterologous nucleic acid into the cell.
The phrase “a corresponding cell grown under essentially the same culture conditions” as used herein means a reference host cell (either engineered or native) which is grown under essentially the same culture conditions, including but not limited to pH, temperature, time, and culture media as compared to an engineered cell encompassed by the invention and to which the reference cell is being compared to.
The term “carbon source” as used herein refers to a substrate that is suitable for use as a source of carbon for cell growth. Carbon sources may include but are not limited to carbohydrates, such as monosaccharides, disaccharides, oligosaccharides; alcohols; aldehydes; amino acids; organic acids; and ketones.
Nucleic acids “hybridize” when they associate, typically in solution. There are numerous texts and other reference materials that provide details regarding hybridization methods for nucleic acids (See e.g., Tijssen, Laboratory Techniques in Biochemistry and Molecular Biology - Hybridization with Nucleic Acid Probes ,” Part 1, Chapter 2, Elsevier, New York, [1993], incorporated herein by reference). For polynucleotides of at least 100 nucleotides in length, low to very high stringency conditions are defined as follows: prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and either 25% formamide for low stringencies, 35% formamide for medium and medium-high stringencies, or 50% formamide for high and very high stringencies, following standard Southern blotting procedures. For polynucleotides of at least 200 nucleotides in length, the carrier material is finally washed three times each for 15 minutes using 2×SSC, 0.2% SDS at least at 50° C. (“low” stringency), at least at 55° C. (“medium” or “moderate” stringency), at least at 60° C. (“medium-high” stringency), at least at 65° C. (“high” stringency), and at least at 70° C. (“very high” stringency). In some embodiments, the stringency conditions include those that: (1) employ low ionic strength and high temperature for washing, for example 0.015 M sodium chloride/0.0015 M sodium citrate/0.1% sodium dodecyl sulfate at 50° C.; (2) employ a denaturing agent during hybridization, such as formamide, for example, 50% (v/v) formamide with 0.1% bovine serum albumin/0.1% Ficoll/0.1% polyvinylpyrrolidone/50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42° C.; or (3) employ 50% formamide, 5×SSC (0.75 M NaCl, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5×Denhardt's solution, sonicated salmon sperm DNA (50 μg/mL), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC (sodium chloride/sodium citrate) and 50% formamide at 55° C., followed by a high-stringency wash consisting of 0.1×SSC containing EDTA at 55° C. In other embodiments, the stringency conditions include overnight incubation at 37° C. in a solution comprising: 20% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 mg/mL denatured sheared salmon sperm DNA, followed by washing the filters in 1×SSC at about 37-50° C. The skilled artisan will recognize how to adjust the temperature, ionic strength, etc. as necessary to accommodate factors to accomplish the desired stringency.
The phrase “naturally-occurring enzyme” as used herein refers to an enzyme having the unmodified amino acid sequence identical to that found in nature (i.e., “wild-type”). Naturally-occurring enzymes include native enzymes (i.e., those enzymes naturally expressed or found in the particular microorganism).
The term “variant” as used herein refers to a polypeptide sequence or polynucleotide sequence encoding a polypeptide, said sequence comprising one or more modifications relative to a corresponding native enzyme (or other specified reference sequence) or the native polynucleotide (or other specified reference sequence) such as substitutions, insertions, deletions, and/or truncations of one or more specific amino acid residues or of one or more specific nucleotides or codons in the polypeptide or polynucleotide. In some embodiments, reference to a variant at an amino acid residue refers to a substitution of the amino acid residue for another amino acid residue. Mutagenesis and directed evolution methods are well known in the art for creating variants (See e.g., U.S. Pat. Nos. 7,783,428, 6,429,175, 6,376,246, 6,586,182, 6,117,679; and Ling, et al., 1999, “Approaches to DNA mutagenesis; an overview,” Anal. Biochem., 254(2):157-78; Smith, 1985, “In vitro mutagenesis,” Ann. Rev. Genet., 19:423-462; Carter, 1986. “Site-directed mutagenesis,” Biochem. J., 237:1-7; Minshull, et al., 1999. “Protein evolution by molecular breeding,” Current Opinion in Chemical Biology, 3:284-290;
The terms “isolated” or “recovered” as used herein refer to a material that is removed from its original environment (e.g., the natural environment, if it is naturally occurring). For example, the material is said to be “isolated” when it is present in a particular composition in a higher or lower concentration than exists in a naturally-occurring or wild-type organism or in combination with components not normally present upon expression from a naturally-occurring or wild-type organism. For example, a naturally-occurring polynucleotide or polypeptide present in a living animal is not isolated, but the same polynucleotide or polypeptide, separated from some or all of the coexisting materials in the natural system, is isolated. In some embodiments, such polynucleotides are part of a vector, and/or such polynucleotides or polypeptides are part of a composition, and still considered to be isolated, in that such vector or composition is not part of its natural environment. In some embodiments, the term isolated refers to fatty alcohol compounds of varying chain lengths which are isolated or recovered from an engineered cell according to the invention.
As used herein, the term “biologically active fragment,” or “functional fragment” refers to a polypeptide that has an amino-terminal and/or carboxy-terminal deletion(s) and/or internal deletion(s), but where the remaining amino acid sequence is identical to the corresponding positions in the sequence to which it is being compared (e.g., a full-length FabB or FAR of the present invention) and that retains substantially all of the activity of the full-length polypeptide. For example, a biologically active fragment can comprise about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% of a full-length FabB or FAR polypeptide. In some embodiments, biologically active fragments comprise 60%, 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% of a full-length FabB or FAR polypeptide.
The term “attenuated” or “attenuation” used interchangeably herein, as applied to a gene refers to any genetic modification that decreases or eliminates the expression of the gene and/or the functional activity of the corresponding gene product (mRNA and/or protein). The term encompasses complete or partial inactivation, suppression, deletion, interruption, blockage, promoter alterations, antisense RNA, dsRNA, or down-regulation of a gene. This can be accomplished, for example, by gene “knockout,” inactivation, mutation (e.g., insertion, deletion, point, or frameshift mutations that disrupt the expression or activity of the gene product), or by use of inhibitory RNAs (e.g., sense, antisense, or RNAi technology).
The term “deletion” in reference to a gene means the elimination or knockout of all or part of a gene's coding sequence. For example, a deletion may encompass (at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70% at least about 80%, at least about 85% 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99%) or all (100%) of the coding sequence of a gene.
With respect to “homologs,” reference to particular gene names is for illustration and not limitation. It is understood that gene names vary from organism to organism and reference to a gene name is not intended to be limiting, but is intended to encompass homologs and polymorphic variants with equivalent activity. In certain embodiments, the invention includes a polynucleotide or polypeptide sequence with at least about 50%, about 55%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% identity with the named gene or gene product.
The terms “peptide,” “polypeptide,” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. In various aspects of the invention, the availability of a polypeptide sequence of a specific enzyme provides a description of all polynucleotides capable of encoding the polypeptide of known sequence because of the known correspondence of particular codons and the amino acids they encode. In certain embodiments, the degeneracy of the genetic code is used to produce a large number of polynucleotides that encode a polypeptide described herein.
“Percentage of sequence identity,” “percent identity,” and “percent identical” are used herein to refer to comparisons between polynucleotide sequences or polypeptide sequences, and are determined by comparing two optimally aligned sequences over a comparison window, wherein the portion of the polynucleotide or polypeptide sequence in the comparison window may comprise additions or deletions (i.e., gaps) as compared to the reference sequence for optimal alignment of the two sequences. The percentage is calculated by determining the number of positions at which either the identical nucleic acid base or amino acid residue occurs in both sequences or a nucleic acid base or amino acid residue is aligned with a gap to yield the number of matched positions, dividing the number of matched positions by the total number of positions in the window of comparison and multiplying the result by 100 to yield the percentage of sequence identity. Determination of optimal alignment and percent sequence identity is performed using the BLAST and BLAST 2.0 algorithms (See e.g., Altschul et al., 1990, J. Mol. Biol. 215: 403-410 and Altschul et al., 1977, Nucleic Acids Res. 3389-3402). Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information website.
Optimal alignment of sequences for comparison and determination of sequence identity can be determined by a sequence comparison algorithm or by visual inspection (see, generally, Ausubel et al., infra). When optimally aligning sequences and determining sequence identity by visual inspection, percent sequence identity is calculated as the number of residues of the test sequence that are identical to the reference sequence divided by the number of non-gap positions and multiplied by 100. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates and sequence algorithm program parameters are designated. The sequence comparison algorithm then calculates the percent sequence identities for the test sequences relative to the reference sequence, based on the program parameters.
An algorithm that may be used to determine whether a variant FabB has sequence identity to SEQ ID NO:2 is the BLAST algorithm, which is described in Altschul et al., 1990 , J. Mol. Biol. 215:403-410, which is incorporated herein by reference. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information (on the worldwide web at ncbi.nlm.nih.gov/). The algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al., 1997. Nucleic Acids Res., 25:3389-3402). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. For amino acid sequences, the BLASTP program uses as defaults a word size (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff & Henikoff, 1989, Proc. Natl. Acad. Sci. USA 89:10915).
Numerous other algorithms are available that function similarly to BLAST in providing percent identity for two sequences. Optimal alignment of sequences for comparison can be conducted (e.g., by the local homology algorithm of Smith and Waterman, 1981, Adv. Appl. Math. 2:482, by the homology alignment algorithm of Needleman and Wunsch, 1970, J. Mol. Biol. 48:443, by the search for similarity method of Pearson and Lipman, 1988, Proc. Natl. Acad. Sci. USA 85:2444, by computerized implementations of these algorithms [GAP, BESTFIT, FASTA, and TFASTA in the GCG Wisconsin Software Package], or by visual inspection [See generally, Current Protocols in Molecular Biology, F. M. Ausubel et al., eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (1995 Supplement)]). Additionally, determination of sequence alignment and percent sequence identity can employ the BESTFIT or GAP programs in the GCG Wisconsin Software package (Accelrys, Madison Wis.), using default parameters provided.
Multiple sequences can be aligned with each other by visual inspection or using a sequence comparison algorithm, such as PSI-BLAST (Altschul, et al., 1997, supra) or “T-Coffee” (Notredame et al., 2000, J. Mol. Bio. 302:205-17). T-Coffee alignments may be carried out using default parameters (T-Coffee Technical Documentation, Version 8.01, July 2009, WorldWideWeb .tcoffee.org), or Protein Align. In Protein Align, alignments are computed by optimizing a function based on residue similarity scores (obtained from applying an amino acid substitution matrix to pairs of aligned residues) and gap penalties. Penalties are imposed for introducing any extending gaps in one sequence with respect to another. The final optimized function value is referred to as the alignment score. When aligning multiple sequences, Protein Align optimizes the “sum of pairs” score, i.e., the sum of all the separate pairwise alignment scores.
As used herein, the terms “numbered with reference to” or “corresponding to,” when used in the context of the numbering of a given polypeptide or polynucleotide sequence, refers to the numbering of the residues of a specified reference sequence when the given amino acid or polynucleotide sequence is compared to the reference sequence. The following nomenclature may be used to describe substitutions in a test sequence relative to a reference sequence polypeptide or nucleic acid sequence: “R-#-V,” where # refers to the position in the reference sequence. R refers to the amino acid (or base) at that position in the reference sequence, and V refers to the amino acid (or base) at that position in the test sequence. In some embodiments, an amino acid (or base) may be called “X,” by which is meant any amino acid (or base).
“Reference sequence” refers to a defined sequence to which another sequence is compared. A reference sequence is not limited to wild-type sequences, and can include engineered or altered sequences. For example, a reference sequence can be a previously engineered or altered amino acid sequence. A reference sequence also may be a subset of a larger sequence, for example, a segment of a full-length gene or polypeptide sequence. Generally, a reference sequence is at least 20 nucleotide or amino acid residues in length, at least 25 residues in length, at least 50 residues in length, or the full length of the nucleic acid or polypeptide. Since two polynucleotides or polypeptides may each (1) comprise a sequence (i.e., a portion of the complete sequence) that is similar between the two sequences, and (2) may further comprise a sequence that is divergent between the two sequences, sequence comparisons between two (or more) polynucleotides or polypeptide are typically performed by comparing sequences of the two polynucleotides over a comparison window to identify and compare local regions of sequence similarity.
As used herein, the term “reference enzyme” refers to an enzyme to which another enzyme of the present invention (e.g., a “test” enzyme, such as wild-type enzyme) is compared in order to determine the presence of an improved property in the other enzyme being evaluated. In some embodiments, a reference enzyme is a wild-type enzyme. In some embodiments, the reference enzyme is an enzyme to which a test enzyme of the present invention is compared in order to determine the presence of an improved property in the test enzyme being evaluated, including but not limited to improved thermoactivity, improved thermostability, and/or improved stability. In some embodiments, a reference enzyme is a wild-type enzyme.
“Comparison window” refers to a conceptual segment of at least about 20 contiguous nucleotide positions or amino acids residues wherein a sequence may be compared to a reference sequence of at least 20 contiguous nucleotides or amino acids and wherein the portion of the sequence in the comparison window may comprise additions or deletions (i.e., gaps) of 20 percent or less as compared to the reference sequence (which does not comprise additions or deletions) for optimal alignment of the two sequences. The comparison window can be longer than 20 contiguous residues, and includes, optionally 30, 40, 50, 100, or longer windows.
As used herein, the term “culturing” refers to growing a population of microbial cells under suitable conditions using any suitable medium (e.g., liquid, solid, or semi-solid media). In particular embodiments, culturing refers to the fermentative bioconversion of a carbon source (e.g., sugar) to an end product (e.g., fatty alcohols).
The term “extracellular environment” means the aqueous solution surrounding a cell membrane, excluding the intracellular space. For example, a secreted enzyme or a compound is found in the extracellular environment. In some embodiments, the extracellular environment comprises the culture medium used to grow the cell.
“Solution” as used herein refers to any medium, phase, or mixture of phases, in which the recombinant host cells and/or products (e.g. fatty alcohols) of the present disclosure are active. It is intended to include purely liquid phase solutions (e.g., aqueous, or aqueous mixtures with co-solvents, including emulsions and separated liquid phases), as well as slurries and other forms of solutions having mixed liquid-solid phases.
The term “contacting” refers to combining an enzyme and a substrate under conditions in which the enzyme can act on the substrate. Those skilled in the art will recognize that mixing a solution containing an enzyme with a substrate will effect “contacting.” Similarly, in the context of culturing microorganisms, culturing microorganisms in a media containing a substrate (e.g., a fermentable sugar) will effect “contacting” the microorganism with the substrate.
“Sugar” as used herein refers to carbohydrate compounds and compositions made up of monosaccharides, disaccharides, trisaccharides, and short chain oligosaccharides (e.g., 6carbon and 5-carbon compounds such as but not limited to glucose, arabinose, fructose, galactose, ribose, mannose, xylose, lactose, sucrose maltose and the like).
The term “fermentable sugars” refers to sugars that a microorganism can utilize for growth or which can be used in the production of end-products such as but not limited to hydrocarbons, amino acids, ethanol and other chemical compounds.
The terms “cleaning compositions” and “cleaning formulations” refer to compositions that find use in the removal of undesired compounds from items to be cleaning, such as fabric, dishes, contact lenses, other solid substrates, hair (shampoos), skin (soaps and creams), teeth (mouthwashes, toothpastes, etc.), etc. The terms further refer to any composition that is suited for cleaning, bleaching, disinfecting and/or sterilizing any object and/or surface. It is intended that the terms include, but are not limited to detergent compositions (e.g., laundry and fine fabric detergents), hard surface cleaning formulations (e.g., for glass, wood, ceramics and metal countertops, windows, etc.), oven cleaners, carpet cleaners, fabric fresheners, fabric softeners, hand and machine dish detergents, dish rinse aids, and textile and laundry pre-spotters. In addition, the terms encompass cleaning compositions for use in household and institutional use, including but not limited to liquid cleaning and disinfecting agents, such as anti-bacterial handsoaps and wipes, cleaning bars, mouthwashes, denture cleaners, car shampoos, bathroom cleaners, hair shampoos and conditioners/rinses for humans and other animals, shower gels, foam baths, etc. Indeed, it is not intended that the term be limited to any particular cleaning composition. The terms encompass any materials/compounds selected for the particular type of cleaning compositions desired and the form of the product (e.g., liquid, gel, granule, or spray), as long as the composition is compatible with the fatty alcohol(s) of the present invention. The specific selection of cleaning composition materials are readily made by considering the surface, item or fabric to be cleaned, and the desired form of the composition for the cleaning conditions during use.
“Corresponding to”, “reference to” or “relative to” when used in the context of the numbering of a given amino acid or polynucleotide sequence refers to the numbering of the residues of a specified reference sequence when the given amino acid or polynucleotide sequence is compared to the reference sequence. In other words, the residue number or residue position of a given polymer is designated with respect to the reference sequence rather than by the actual numerical position of the residue within the given amino acid or polynucleotide sequence. For example, a given amino acid sequence, such as that of an engineered enzyme, can be aligned to a reference sequence by introducing gaps to optimize residue matches between the two sequences. In these cases, although the gaps are present, the numbering of the residue in the given amino acid or polynucleotide sequence is made with respect to the reference sequence to which it has been aligned.
“Different from” or “differs from” with respect to a designated reference sequence refers to difference of a given amino acid or polynucleotide sequence when aligned to the reference sequence. Generally, the differences can be determined when the two sequences are optimally aligned. Differences include insertions, deletions, or substitutions of amino acid residues in comparison to the reference sequence. Typically, the reference sequence is a naturally occurring sequence from which the sequence with the differences is derived. The present disclosure provides engineered pathways of enzymes, wherein the enzymes are encoded by one or more recombinant polynucleotides having one or more nucleotide sequence differences relative to a reference polynucleotide sequence, which is typically the corresponding naturally occurring polynucleotide from which the recombinant polynucleotide is derived. Further, the nucleotide differences may encode one or more amino acid residue differences in the enzymes, where the encoded amino acid differences, which can include either/or both conservative and non-conservative amino acid substitutions.
“Derived from” as used herein in the context of engineered enzymes, identifies the originating enzyme, and/or the gene encoding such enzyme, upon which the engineering was based.
“Amino acid residue” or “amino acid” or “residue” as used herein refers to the specific monomer at a sequence position of a polypeptide.
“Conservative amino acid substitution” refers to a substitution of a residue with a different residue having a similar side chain, and thus typically involves substitution of the amino acid in the polypeptide with amino acids within the same or similar defined class of amino acids. By way of example and not limitation, an amino acid with an aliphatic side chain may be substituted with another aliphatic amino acid (e.g., alanine, valine, leucine, and isoleucine); an amino acid with hydroxyl side chain is substituted with another amino acid with a hydroxyl side chain (e.g., serine and threonine); an amino acids having aromatic side chains is substituted with another amino acid having an aromatic side chain (e.g., phenylalanine, tyrosine, tryptophan, and histidine); an amino acid with a basic side chain is substituted with another amino acid with a basic side chain (e.g., lysine and arginine); an amino acid with an acidic side chain is substituted with another amino acid with an acidic side chain (e.g., aspartic acid or glutamic acid); and a hydrophobic or hydrophilic amino acid is replaced with another hydrophobic or hydrophilic amino acid, respectively.
“Non-conservative substitution” refers to substitution of an amino acid in a polypeptide with an amino acid with significantly differing side chain properties. Non-conservative substitutions may use amino acids between, rather than within, the defined groups and affects (a) the structure of the peptide backbone in the area of the substitution (e.g., proline for glycine) (b) the charge or hydrophobicity, or (c) the bulk of the side chain. By way of example and not limitation, an exemplary non-conservative substitution can be an acidic amino acid substituted with a basic or aliphatic amino acid; an aromatic amino acid substituted with a small amino acid; and a hydrophilic amino acid substituted with a hydrophobic amino acid.
“Deletion” when used with reference to a polypeptide refers to modification of the polypeptide by removal of one or more amino acids from a reference polypeptide. Deletions can comprise removal of 1 or more amino acids, 2 or more amino acids, 5 or more amino acids, 10 or more amino acids, 15 or more amino acids, or 20 or more amino acids, up to 10% of the total number of amino acids, up to 20% of the total number of amino acids, and up to 30% of the total number of amino acids making up the polypeptide while retaining enzymatic activity and/or retaining the improved properties of an engineered enzyme. Deletions can be directed to the internal portions and/or terminal portions of the polypeptide. In various embodiments, the deletion can comprise a continuous segment or can be discontinuous.
“Insertion” when used with reference to a polypeptide refers to modification of the polypeptide by addition of one or more amino acids to the reference polypeptide. In some embodiments, the improved engineered enzymes comprise insertions of one or more amino acids relative to the corresponding naturally occurring polypeptide as well as insertions of one or more amino acids to other improved polypeptides. Insertions can be in the internal portions of the polypeptide, or to the carboxy or amino terminus. Insertions as used herein include fusion proteins as is known in the art. The insertion can be a contiguous segment of amino acids or separated by one or more of the amino acids in the naturally occurring polypeptide.
“Improved property” as used herein refers to a functional characteristic of an enzyme or host cell that is improved relative to the same functional characteristic of a reference enzyme or reference host cell. Improved properties of the engineered enzymes or host cells comprising engineered pathways disclosed herein can include but are not limited to: increased activity (including increased rate conversion of substrate to product, or increased percentage conversion in a period of time), altered substrate specificity, and/or preference, increased expression, as well as increased secretion.
“Codon optimized” refers to changes in the codons of the polynucleotide encoding a protein to those preferentially used in a particular organism such that the encoded protein is efficiently expressed in the organism of interest. In some embodiments, the polynucleotides encoding the enzymes used in the engineered pathways of the present disclosure may be codon optimized for optimal production from the host organism selected for expression.
DETAILED DESCRIPTION OF THE INVENTION
The recombinant host cells, engineered pathways, and specific recombinant polynucleotides and encoded enzymes that make up the pathways and carry out the substrate-to-product conversions are described in greater detail below. Additionally, the following sections describe methods for using the recombinant host cells for the production of fatty alcohols and particularly fatty alcohol compositions comprising at least 25% C12 and C14C fatty alcohols.
FabB Variant Enzymes
In one embodiment, the invention is directed to fatty alcohol compositions comprising predominantly C12 to C14 fatty alcohols produced from engineered microbial cells.
To obtain the above objective, in certain embodiments a polynucleotide sequence encoding a variant FabB enzyme has been introduced into an engineered cell (e.g., a bacterial cell). FabB is one of the critical fatty acid biosynthetic genes involved in the collection of reactions responsible for fatty acid elongation. The fatty acids may then be used as intermediates in the production of fatty alcohols, aldehydes, esters, and the like. In one embodiment a variant FabB according to the invention when introduced and expressed by a host cell may increase the saturation level of the produced fatty acid intermediates as compared to a corresponding host cell.
In certain embodiments, the polynucleotide sequence encoding a variant FabB enzyme will comprise a nucleic acid sequence that is at least about 70%, about 71%, about 72%, about 73%, about 74%, about 75%, about 76%, about 77%, about 78%, about 79%, about 80%, about 81%, about 82%, about 83%, about 84%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% identical to SEQ ID NO: 1. In some embodiments, the variant FabB is encoded by a nucleic acid sequence that can selectively hybridize to SEQ ID NO: 1 under moderately stringent, stringent or highly stringent conditions, as described hereinabove.
In some embodiments, the variant FabB comprises an amino acid sequence that is a least about 80%, about 85%, about 86%, about 87%, about 88%, about 89%, about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or 99% identical to the amino acid sequence set forth in SEQ ID NO:2. In some embodiments, the FabB is a functional fragment of a sequence having at least about 90% sequence identity to SEQ ID NO:2. In some embodiments, the functional fragment is about 90%, about 91%, about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, or about 99% the length of the sequence of SEQ ID NO:2.
In some embodiments, the variant FabB comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more positions associated with the binding pocket and/or the surface of a FabB protein of SEQ ID NO:2. The structure of the acyl-binding pocket and the active site of KAS enzymes is discussed in Olsen et al. 2001 Structure 9: 233-234.
In some embodiments, the variant FabB comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2.
In some embodiments, the variant FabB comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions R30, T34, E38, K40, S42, G43, R45, N51, K53, D61, R62, K63, V64, V65, R66, N95, N96, P97, G106, G107, G108, P110, R111, F112, Q113, V114, F115, G116, A117, R124, K127, A128, G130, P131, Y132, V133, V134, T135, K136, A137, M138, A139, S140, P149, K151, S161, A162, A164, T165, E191, E196, M197, E200, F201, M204, G205, A206, T209, K210, N212, E216, K217, A224, H225, R226, F229, I231, A267, D268, V270, A271, P272, S273, E275, K282, M285, H298, T300, T302, P303, V304, G305, K308, A311, R314, E315, H333, L335, G336, N360, I361, Q367, F390, G391, F392, or N396 when optimally aligned with SEQ ID NO:2.
In some embodiments, the variant FabB comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions K40, S42, R45, G106, G107, G108, P110, Q113, A117, V133, V134, A137, M138, M197, E200, F201, A206, E216, K217, I231, T300, T302, P303, G305, L335, or Q367 when optimally aligned with SEQ ID NO:2.
In some embodiments, the variant FabB comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions 40, 42, 45, 206, 231, 216, 217, 303, 335 or 367 when optimally aligned with SEQ ID NO:2. In some embodiments, the variant FabB will comprise at least 85% (also at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions K40, S42, R45, A206, E216, K217, I231, P303, L335, or Q367 when optimally aligned with the polypeptide of SEQ ID NO:2.
In other embodiments, the variant FabB will comprises one or more of the following substitutions K40G/R/A/S, S42E/L, R45G, A206G, I231F/C, E216W, K217V, P303C/V, L335M, or Q367V.
In some embodiments, a FabB variant will include more than 1 substitution such as 2, 3, 4, 5, 6, 7, 8 or more substitutions when aligned with a FabB polypeptide having at least 85% (also at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) sequence identity to SEQ ID NO:2. In some embodiments, the FabB variant will be a functional fragment having at least 85% (also at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) sequence identity to SEQ ID NO:2 and comprising a substitution of one or more of the following positions R30, T34, E38, K40, S42, G43, R45, N51, K53, D61, R62, K63, V64, V65, R66, N95, N96, P97, G106, G107, G108, P110, R111, F112, Q113, V114, F115, G116, A117, R124, K127, A128, G130, P131, Y132, V133, V134, T135, K136, A137, M138, A139, S140, P149, K151, S161, A162, A164, T165, E191, E196, M197, E200, F201, M204, G205, A206, T209, K210, N212, E216, K217, A224, H225, R226, F229, I231, A267, D268, V270, A271, P272, S273, E275, K282, M285, H298, T300, T302, P303, V304, G305, K308, A311, R314, E315, H333, L335, G336, N360, I361, Q367, F390, G391, F392, or N396 when optimally aligned with SEQ ID NO:2. In other embodiments, the FabB variant will be a functional fragment having at least 85% (also at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) sequence identity to SEQ ID NO:2 and comprising a substitution of one or more of the following positions 40, 42, 45, 206, 231, 216, 217, 303, 335 or 367 when optimally aligned with SEQ ID NO:2. In some embodiments the functional fragment of a FabB variant is about 90%, (such as 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or even 99%) the length of the sequence of SEQ ID NO:2. In some embodiments, the FabB variant may be truncated at the amino and/or carboxy end of the polypeptide.
FabB sequences homologous to SEQ ID NO:2 can be identified by any of a variety of methods known in the art. For example, a sequence alignment can be conducted against a database, for example against the NCBI database, and sequences with the lowest HMM E-value can be selected. One could then test whether or not these identified sequences have FabB activity and reference is made to Edwards et al., 1997, FEBS let. 402: 62-66.
In another aspect, the present invention provides polynucleotides encoding the variant FabB enzymes as described above. The polynucleotide can be a DNA or RNA and can be single-stranded or double-stranded. The polynucleotides may be prepared wholly or partially via synthetic means. In various aspects of the invention, the availability of a polypeptide sequence of a specific variant FabB enzyme provides a description of all polynucleotides capable of encoding the polypeptide of the known sequence because of the known correspondence of particular codons and the amino acids they encode. In certain embodiments, the degeneracy of the genetic code is used to produce a large number of polynucleotides that encode the variant FabB polypeptides described herein. In some embodiments, the polynucleotides encoding the desired enzyme are codon-optimized. In particular embodiments, the polynucleotides that encode the FabB enzymes described herein are codon-optimized for expression in a bacterial host cell. Indeed, it is intended that the polynucleotides of the present invention be produced using any suitable methods and components as known in the art.
DNA Constructs and Vectors
In some embodiments, the variant FabB polynucleotide is introduce into a host cell on a DNA construct or vector. In another aspect, the present invention provides polynucleotides encoding the variant FabB enzymes as described above. The polynucleotide can be a DNA or RNA and can be single-stranded or double-stranded. The polynucleotide can be isolated from a naturally occurring microorganism, or prepared wholly or partially via synthetic means. Indeed, it is intended that the polynucleotides of the present invention be produced using any suitable methods and components as known in the art.
In various aspects of the invention, the availability of a polypeptide sequence of a specific variant FabB enzyme provides a description of all polynucleotides capable of encoding the polypeptide of the known sequence because of the known correspondence of particular codons and the amino acids they encode. In certain embodiments, the degeneracy of the genetic code is used to produce a large number of polynucleotides that encode the variant FabB polypeptides described herein. In some embodiments, the polynucleotides encoding the desired enzyme are codon-optimized. In particular embodiments, the polynucleotides that encode the FabB enzymes described herein are codon-optimized for expression in a bacterial host cell.
In certain embodiments, the present disclosure provides a vector or DNA construct comprising a polynucleotide encoding a variant FabB enzyme as described above, wherein the variant FabB is produced in a host cell. In some embodiments, the polynucleotide is a codon-optimized polynucleotide, such as a polynucleotide having at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or even about 100% sequence identity to SEQ ID NO: 1.
Any suitable DNA construct and vector finds use in the invention (See e.g., WO 2011/008535 which is here in incorporated by reference). In some embodiments, the polynucleotide encodes for a variant FabB enzyme and the polynucleotide sequence will have at least about 90%, at least about 91%, at least about 92%, at least 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, at least about 99%, or even about 100%) sequence identity to SEQ ID NO: 1.
In some embodiments, polynucleotides encoding a variant FabB as described herein for expression in the recombinant host cells are operably linked to a promoter, and optionally, to other control sequences. Suitable promoters include, but are not limited to constitutive promoters, regulated promoters, and inducible promoters. Appropriate promoter sequences can be obtained from genes encoding extracellular or intracellular polypeptides which are either endogenous or heterologous to the host cell. Methods for the isolation, identification and manipulation of promoters of varying strengths are available in or readily adapted from the art (See e.g., Nevoigt et al. (2006) Appl. Environ. Microbiol. 72:5266-5273, the disclosure of which is herein incorporated by reference in its entirety). In certain embodiments, the DNA constructs, vectors and polynucleotides are suitable for expression of a variant FabB in bacteria. For bacterial host cells, suitable promoters for directing transcription of the nucleic acid constructs of the present disclosure, include, but are not limited to the promoters obtained or derived the E. coli lac operon, Streptomyces coelicolor agarase gene (dagA), Bacillus subtilis levansucrase gene (sacB), Bacillus licheniformis alpha-amylase gene (amyL), Bacillus stearothermophilus maltogenic amylase gene (amyM), Bacillus amyloliquefaciens alpha-amylase gene (amyQ), Bacillus licheniformis penicillinase gene (penP), Bacillus subtilis xylA and xylB genes, Bacillus megaterium promoters, and prokaryotic beta-lactamase gene (Villa-Kamaroff et al., Proc. Natl Acad. Sci. USA 75: 3727-3731 (1978)), as well as the tac promoter (DeBoer et al., Proc. Natl Acad. Sci. USA 80: 21-25 (1993)). Additional promoters include trp promoter, phage lambda PL, T7 promoter, promoters found at PromEC (at website margalit.huji.ac.il/promec/index.html) and the like. Particularly useful promoters include the Trc promoter (Brosius J. et al., (1985) J. Biol. Chem. 260: 3539-3541). In certain embodiments, the promoter that is used in a vector or DNA construct is the native fabB promoter. In some embodiments, when the variant fabB is chromosomally integrated in a host cell, expression is regulated by the native fabB promoter. Additional promoters suitable for use in the present disclosure are described in Terpe H., 2006, Appl. Microbiol. Biotechnol. 72:211-222 and in Sambrook et al (2001) Molecular Cloning: A Laboratory Manual, 3 rd ed., Cold Spring Harbor Laboratory Press, New York.
In various embodiments, an expression vector optionally contains a ribosome binding site (RBS) for translation initiation, and a transcription terminator, such as the transcriptional terminators T 1 and T 2 derived from the rrnB operon from E. coli (See e.g., Orosz et al., (1991) Eur. J. Biochem. 201: 653-659). The vector also optionally includes appropriate sequences for amplifying expression (e.g., translational enhancers).
In various embodiments, the polynucleotides useful for expressing the heterologous enzymes in recombinant host cells are operably linked to other control sequences, including but not limited to, a transcription terminator sequence, a signal sequence that when translated directs the expressed polypeptide into the secretory pathway of the recombinant host cell, and/or a polyadenylation sequence (eukaryotes). The choice of appropriate control sequences for use in the polynucleotide constructs of the present disclosure is within the skill in the art and in various embodiments is dependent on the recombinant host cell used and the desired method of recovering the fatty alcohol compositions produced. Indeed, it is not intended that the present invention be limited to any particular control sequence(s).
A recombinant expression vector according to the invention can be any suitable vector (e.g., a plasmid or a virus), which can be manipulated by recombinant DNA techniques to facilitate expression of at least one heterologous enzyme in the recombinant host cell. In certain preferred embodiments, the expression vector is integrated into the chromosome of the host cell and comprises one or more heterologous genes operably linked to one or more control sequences useful for production of a variant FabB enzyme. In other embodiments, the expression vector is an extra chromosomal replicative DNA molecule (e.g., a linear or closed circular plasmid), that is found either in low copy number (e.g., from about 1 to about 10 copies per genome equivalent) or in high copy number (e.g., more than about 10 copies per genome equivalent). In various embodiments, the expression vector includes a selectable marker, such as a gene that confers antibiotic resistance (e.g., ampicillin, kanamycin, chloramphenicol or tetracycline resistance) to the recombinant host organism that comprises the vector.
Expression vectors that, in certain embodiments, are useful for expressing enzymes as disclosed herein are commercially available (e.g., from Sigma-Aldrich Chemicals, St. Louis Mo. and Stratagene, LaJolla Calif.). In some embodiments, examples of suitable expression vectors are plasmids which are derived from pBR322 (Gibco BRL), pUC (Gibco BRL), pREP4, pCEP4 (Invitrogen) or pPoly (Lathe et al., 1987 , Gene 57:193-201).
In certain embodiments, the present disclosure provides a plasmid for expression of heterologous genes in E. coli (e.g. the introduction of a FAR polynucleotide). Expression vector pCK110900, which comprises a P15A origin of replication “ori” (P15A ori), lac a CAP binding site, a lac promoter, a T7 ribosomal binding site (T7g10 RBS) and a chloramphenicol resistance gene (camR) is an exemplary vector that finds use in the present invention. This expression vector is depicted in FIG. 3 of U.S. Patent Publication No. 2006/0195947, which is incorporated herein by reference in its entirety. Other suitable plasmid vectors include, but are not limited to derivatives of pCL1920 and pCL1921 (Lerner and Inouye, 1990; NAR 18:4631). These vectors contain the pSC101 ori and confer resistance to spectinomycin (GenBank:AB236930). In some embodiments, the vector is an expression vector derived from pCL1920 including the Trc promoter and the lacIq gene from E. coli.
Modification of Native β-Ketoacyl-ACP Synthase Genes
In one embodiment, a native β-ketoacyl-ACP synthase is modified at the native locus on the genome. In some embodiments the native β-ketoacyl-ACP synthase is a β-ketoacyl-ACP synthase I coded for by a fabB gene and in some embodiments, the native β-ketoacyl-ACP synthase is a β-ketoacyl-ACP synthase II coded for by a fabF gene. In some embodiments, the general term “native gene modification” is used to broadly encompass the various types of alterations that result in modification of a native β-ketoacyl-ACP synthase. One of skill in the art is well aware of many methods that may be used to modify a native β-ketoacyl-ACP synthase. For example one or more of the following non-limiting methods may be used to accomplish native gene modification: 1) complete replacement of the native fabB gene by a variant fabB polynucleotide encoding a variant Fab enzyme as described herein above; 2) replacement of the native fabB gene by a fab gene from a microorganism other than the host microorganism (e.g. by a β-ketoacyl-ACP synthase (fabF or fabB) from Lactococcus lactis or a β-ketoacyl-ACP synthase from plasmodium falciparum ) followed by modification of the gene; 3) partial replacement of the native fabB gene (e.g., replacement of at least 20%, at least 25%, at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 55%, at least 60%, at least 65%, at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 95% and at least 99%) of the native fabB gene, 4) mutations (such as substitutions) of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50 or more amino acid residues coded for by a nucleic acid in the native fabB gene, wherein the mutations are at the native FabB chromosomal locus, 5) introduction of a homologous or heterologous fabB gene which is then optionally optimized; and 6) hybrid gene replacement, wherein part of the native gene is truncated and replaced with a polynucleotide sequence introduced into the host cell comprising a substitution in the coding region of the fabB gene. The truncation may be at the 5′ or 3′ end of the coding sequence. Methods of gene targeting and replacement are known. Most commonly this method is accomplished by homologous recombination of similar nucleic acids and reference is made to C. M. Hamilton et al., J. Bacteriol. (1989) 171:4617-4622; N. R. De Lay et al., J. Bacteriol. (2006) 188:287-296; Y -G Kim et al., BioTechniques (2000) 28:198-204; and A. J. Link et al., J. Bacteriol. (1997) 179:6228-6237.
In certain embodiments, the skilled person could use a Lactocococcus lactis fabF to functionally replace the native fabF and/or fabB in E. coli (See, Morgan-Kiss and Cronan (2008) Arch. Microbiol. 190:427-437).
In some embodiments, the native gene modification encompasses the entire coding region of the native fabB gene. In some embodiments, the native FabB gene will have a nucleic acid sequence that is at least 85% (at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% and even 100%) identical to the polynucleotide sequence of SEQ ID NO: 1. In some preferred embodiments, the native gene modification of a native fabB gene is replacement with a polynucleotide encoding a variant FabB or with a mutation made at the gene level (such as, but not limited to, one or more point mutations) and this modification is stable over many generations.
Transformation of Host Cells
Methods, reagents and tools for transforming host cells described herein, such as bacteria, yeast (including oleaginous yeast) and filamentous fungi are known in the art. General methods, reagents and tools for transforming (e.g., bacteria) can be found, for example, in Sambrook et al (2001) Molecular Cloning: A Laboratory, Manual, 3 rd ed., Cold Spring Harbor Laboratory Press, New York. Methods, reagents and tools for transforming yeast are described in “Guide to Yeast Genetics and Molecular Biology,” C. Guthrie and G. Fink, Eds., Methods in Enzymology 350 (Academic Press, San Diego, 2002). Methods, reagents and tools for transforming Y. lipolytica are found in “ Yarrowia lipolytica ,” C. Madzak, J. M. Nicaud and C. Gaillardin in “Production of Recombinant Proteins Novel Microbial and Eucaryotic Expression Systems,” G. Gellissen, Ed. 2005. Methods for engineering cyanobacteria may be found in T. Thiel (1994), Genetic analysis of cyanobacteria, in D. Bryant (ed.) The Molecular Biology of Cyanobacteria, Kluwer Academic Publishers 581-611. In some embodiments, introduction of the DNA construct or vector of the present invention into a host cell is accomplished by calcium phosphate transfection, DEAE-dextran mediated transfection, electroporation, or other common techniques (See Davis et al., 1986 , Basic Methods in Molecular Biology , which is incorporated herein by reference). In one embodiment, a preferred method used to transform E. coli strains is electroporation and reference is made to Dower et al., 1988) NAR 16: 6127-6145. Indeed, any suitable method for transforming host cells finds use in the present invention. It is not intended that the present invention be limited to any particular method for introducing nucleic acids such as constructs into host cells.
The present invention also provides a method for producing an engineered cell, comprising: (a) providing a DNA construct comprising a polynucleotide sequence encoding a variant FabB enzyme operably linked to a promoter and (b) transforming a host cell with the DNA construct, wherein the polynucleotide sequence encoding the variant FabB replaces the endogenous fabB gene. In some embodiments, the host cell is a bacterial cell and in other embodiments, the host cell is E. coli . In some embodiments, the host cell already comprises a polynucleotide sequence encoding a heterologous FAR enzyme as described herein (i.e., the host cell comprises the FAR sequence prior to transformation). In some embodiments the variant FabB polynucleotide and the heterologous FAR polynucleotide will be co-transformed on the same or different construct or plasmid.
Host Cells
In some embodiments, the host cell is a prokaryotic cell. Suitable prokaryotic cells include Gram-positive, Gram negative and Gram-variable bacterial cells. In certain embodiments, host cells include, but are not limited to, species of a genus selected from the group of Acetobacter, Acinetobacter, Agrobacterium, Arthrobacter, Bacillus, Clostridium, Cornebacterium, Desulfovibrio, Escherichia, Erwinia, Geobacillus, Klebsiella, Lactobacillus, Mycobacterium, Pantoea, Rhodococcus, Rhodobacter, Streptomyces, Vibrio , and Zymomonas.
In certain embodiments, the recombinant host cell is an industrial bacterial strain. Numerous bacterial industrial strains are known and suitable for use in the methods disclosed herein. In some embodiments, the bacterial host cell is a species of the genus Bacillus (e.g., B. thuringiensis, B. anthracis, B. megaterium, B. subtilis, B. lentus, B. circulans, B. pumilus, B. lautus, B. coagulans, B. brevis, B. firmus, B. alkalophilus, B. licheniformis, B. clausii, B. stearothermophilus, B. halodurans, B. subtilis, B. pumilus , or B. amyloliquefaciens ). In some embodiments, the bacterial host cell is a species of the genus Erwinia (e.g., E. uredovora, E. carotovora, E. ananas, E. herbicola, E. punctata , or E. terreus ). In other embodiments the bacterial host cell is a species of the genus Pantoea (e.g., P. citrea or P. agglomerans ). In still other embodiments, the bacterial host cell is a species of the genus Streptomyces (e.g., S. ambofaciens, S. achromogenes, S. avermitilis, S. coelicolor, S. aureofaciens, S. aureus, S. fungicidicus, S. griseus , or S. lividans ). In further embodiments, the bacterial host cell is a species of the genus Zymomonas (e.g., Z. mobils or Z. lipolytica ). In further embodiments, the bacterial host cell is a species of the genus Rhodococcus (e.g. R. opacus ).
In some embodiments, the bacterial host cell is a species of the genus Escherichia (e.g., E. coli ). In various embodiments, the engineered E. coli bacterial strains useful in the processes described herein are derived from strain W3110, strain MG1655, strain B766 ( E. coli W) and strain BW25113. In some further embodiments, the W3110 strain finds use in the present invention; the genome of this strain has been fully sequenced and annotated (See e.g., Hayashi et al., (2005) Mol. Syst. Biol. 2:2006.0007). For industrial applications, phage-resistant strains are particularly useful. In this sense, deletion of the fhuA gene (also known as tonA) confers resistance to phages T1, T5 and phi80 (Link et al., 1997, J. Bact. 179: 6228-8237). Another useful strain is E. coli W (Archer et al., 2011, BMC Genomics, 12:9.doi:10.1186/1471-2164-12-9). Also reference is made to Elben et al. (2005) J. of Food Protection 68(2):282-291. In some embodiments, the engineered host cell according to the invention will be a bacterial strain already including the deletion of one or more non-essential genes. For example Baba et al., 2006, in Molecular Systems Biology doi: 10.1038/msb4100050 describes the deletion of various chromosomal genes in E. coli . In certain embodiments, the host cell is a cell that has already been engineered to include one or more heterologous genes such as for example a gene encoding a FAR, a gene encoding a Fab enzyme, or a gene encoding a thioesterase.
Other examples of useful E. coli strains include, but are not limited to, E. coli strains found in the E. coli Stock Center from Yale University (at website cgsc.biology.yale. edu/index.php); the Keio Collection, available from the National BioResource Project at NBRP E. coli , Microbial Genetics Laboratory, National Institute of Genetics 1111 Yata, Mishima, Shizuoka, 411-8540 Japan (www at shigen.nig.ac.jp/ecoli/strain/top/top.jsp); or strains deposited at the American Type Culture Collection (ATCC).
The disclosed invention further contemplates the use of other organism such as photosynthetic organisms including but not limited to algae (e.g., cyanobacteria (blue green algae) and photosynthetic bacteria. Non-limiting examples include strains of Synechococcus, Synechocystis, Rhodobacter, Rhodococcus, Chlamydomonas, Chlorella, Prototheca, Cyanobacterium, Ralstonia, Alcaligenes, Rhodopseudomonas , and Saccharophagus degradans . Additional heterotrophic species which may be used as host strains that are transformed with genes encoding enzymes involved in the synthesis of fatty alcohols include yeast cells such as but not limited to Saccharomyces (e.g., S. cerevisiae ), Yarrowia (e.g., Y. lipolytica ), Candida (e.g., C. troplicalis ) and Schizosaccharomyces (e.g., S. pombe ).
Fatty Alcohol Forming Acyl-CoA Reductases (FARs)
In some embodiments, the engineered cells encompassed by the invention are modified to express a polynucleotide encoding a heterologous FAR. Polynucleotides encoding FAR enzymes are known in the art (See e.g., WO2011/008535; WO2011/019858; U.S. Ser. No. 13/171,138, US2010/02036; U.S. Pat. Nos. 7,332,311; 6,143,538 and Metz et al., 2000, Plant Physiol. 122:635-644).
In some embodiments the acyl-CoA is reduced to a fatty alcohol in a two-step process. A NAD(P)H dependent acyl-CoA reductase converts an acyl-CoA to a fatty aldehyde and then the fatty aldehyde is reduced to a fatty alcohol by a NAD(P)H dependent alcohol dehydrogenase. Enzymes involved in this two-step conversion include for example the enzymes Acrl and YqhD. (See, Reiser and Somerville, J. Bacteriol. (1997) 179:2969; Ishige et al., Appl. Environ. Microbiol. (2000) 66:3481; Hofrander et al. (2011) FEBS Letters 585:3538-3543 and Kalscheuer et al., 2006, Appl. Environ. Microbiol. 72:1373). IN some embodiments, these enzyme use acyl-CoAs as substrates.
In some embodiments, the FARs useful in the present invention catalyze the direct reduction of acyl-CoA and/or acyl-ACP substrates to fatty alcohols wherein free fatty aldehydes are essentially not released as an intermediate. Essentially these FARs reduce acyl chains to fatty alcohols by one enzymatic step. Depending on the substrate chain length it is possible to have trace amounts of aldehydes produced and released. In some embodiments of the direct reduction process, FAR converts at least acyl-ACP substrates to a fatty alcohol end-product without the subsequent action of an alcohol dehydrogenase.
In some embodiments, the FAR is a prokaryotic enzyme. In some embodiments the FAR is derived from a species of Marinobacter including, but not limited to, M. algicola, M. alkaliphilus, M. aquaeolei, M. arcticus, M. bryozoorum, M. daepoensis, M. excellens, M. flavimaris, M. guadonensis, M. hydrocarbonoclasticus, M. koreenis, M. lipolyticus, M. litoralis, M. lutaoensis, M. maritimus, M. sediminum, M. squalenivirans , and M. vinifirmus , and equivalent and synonymous species thereof.
In certain embodiments, an engineered host cell will comprise a) a modified native fabB polynucleotide sequence encoding a FabB which comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2 and b) a heterologous FAR as herein described. In certain embodiments, the engineered host cell (e.g., a bacterial cell) will comprises a) a fabB polynucleotide sequence encoding a variant FabB which comprises at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions R30, T34, E38, K40, S42, G43, R45, N51, K53, D61, R62, K63, V64, V65, R66, N95, N96, P97, G106, G107, G108, P110, R111, F112, Q113, V114, F115, G116, A117, R124, K127, A128, G130, P131, Y132, V133, V134, T135, K136, A137, M138, A139, S140, P149, K151, S161, A162, A164, T165, E191, E196, M197, E200, F201, M204, G205, A206, T209, K210, N212, E216, K217, A224, H225, R226, F229, I231, A267, D268, V270, A271, P272, S273, E275, K282, M285, H298, T300, T302, P303, V304, G305, K308, A311, R314, E315, H333, L335, G336, N360, I361, Q367, F390, G391, F392, or N396 when optimally aligned with SEQ ID NO:2 and b) a heterologous FAR.
In certain embodiments, the heterologous FAR is derived from M. algicola strain DG893 and has an amino acid sequence that is at least about 70% identical, at least about 75%, at least about 80% identical, at least about 85% identical, at least about 90% identical, at least about 93% identical at least about 95% identical, at least about 97% identical, at least about 98% identical and/or at least about 99% identical to SEQ ID NO:4 and/or a functional fragment thereof. In certain embodiments, the FAR is a variant of the wild-type FAR of SEQ ID NO:2 for example a FAR having at least 85%, (at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% 99% and even 100% sequence identity to SEQ ID NO:6 or SEQ ID NO:10. In some embodiments, the FAR variants will have at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 12, at least 14, at least 16, at least 18, at least 20 at least 25 or more amino acid alterations (e.g., substitutions, deletions and/or insertions) relative to SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO: 10.
In certain embodiments, the FAR is derived from Marinobacter aquaeolei . In certain embodiments the FAR derived from M. aquaeolei has an amino acid sequence that is at least about 70% identical, at least about 75%, at least about 80% identical, at least about 85% identical, at least about 90% identical, at least about 93% identical, at least about 95% identical, at least about 97% identical, at least about 98% identical and/or at least about 99% identical to SEQ ID NO:5 as disclosed in WO 2012/006114 and/or a functional fragment thereof. In another specific embodiment, the FAR enzyme has an amino acid sequence that is identical to SEQ ID NO:5. In certain embodiments, the FAR is a variant of the wild-type FAR of SEQ ID NO:5 that has at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 12, at least 14, at least 16, at least 18, at least 20, or more amino acid alterations (e.g., substitutions, deletions and/or insertions) relative to SEQ ID NO:5. In certain embodiments, the FAR is encoded by a polynucleotide sequence having at least 85% (at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% sequence identity to SEQ ID NO:4 as disclosed in WO 2012/006114. In other embodiments, the FAR derived from M. aquaeolei has an amino acid sequence that is at least about 70% identical, at least about 75%, at least about 80% identical, at least about 85% identical, at least about 90% identical, at least about 93% identical, at least about 95% identical, at least about 97% identical, at least about 98% identical, at least about 99% identical and even 100% identical to SEQ ID NO:1 as disclosed in us 2012/0184006 and/or a functional fragment thereof such as amino acids 1 to 364 or amino acids 365 to 591 of SEQ ID NO: 1). In certain embodiments, the FAR is a variant of the wild-type FAR of SEQ ID NO:2 that has at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, at least 8, at least 9, at least 10, at least 12, at least 14, at least 16, at least 18, at least 20, or more amino acid alterations (e.g., substitutions, deletions and/or insertions) relative to SEQ ID NO:2.
In certain embodiments, the FAR is obtained from a marine bacterium selected from the group of Meptuniibacter caesariensis strain MED92 , Reinekea sp. strain MED297 , Marinomonas sp. strain MED121, unnamed Gammaproteobacteria strain HTCC2207, and Marinobacter sp. strain ELB17, as well as equivalents and synonymous species thereof. In certain embodiments, the FAR is obtained from the genus Oceanobacter . In some embodiments, the FAR is obtained from the Oceanobacter species strain RED65 (e.g. NCBI accession number ZP_01305629) and has an amino acid sequence that is at least about 70% identical, at least about 75% identical, at least about 80% identical, at least about 85% identical, at least about 90% identical, at least about 93% identical, at least about 95% identical, at least about 97% identical, at least about 98% identical and/or at least about 99% identical to SEQ ID NOs:6 and/or 8 as disclosed in WO 2011/008535.
In various embodiments, the FAR has the sequence or is encoded by a polynucleotide selected from the group of FAR_Hch ( Hahella chejuensis KCTC 2396, GenBank YP_436183) (SEQ ID NO:29); FAR_Mac (from marine Actinobacterium strain PHSC20C1) (SEQ ID NO:30); FAR_JVC (JCVI_ORF_1096697648832, GenBank Accession No. EDD40059.1) (SEQ ID NO:31); FAR_Fer (JCVI_SCAF_1101670217388) (SEQ ID NO:32); FAR_Key (JCVI_SCAF_1097205236585) (SEQ ID NO:33); FAR_Gal (JCVI_SCAF_1101670289386) (SEQ ID NO:34); Vitis vinifera FAR (GenBank Accession No. CAO22305.1 [SEQ ID NO:35] or CAO67776.1 [SEQ ID NO:36]); Desulfatibacillum alkenivorans FAR (GenBank Accession No. NZ_ABII01000018.1); Stigmatella aurantiaca FAR (NZ_AAMD01000005.1) (SEQ ID NO:37); Phytophthora ramorum FAR (GenBank Accession No.: AAQX01001105.1) (SEQ ID NO:38); Simmondsia chinensis acyl CoA reductase (GenBank Accession no. AAD38039.1) (SEQ ID NO:39); Bombyx mori fatty-acyl reductase (GenBank Accession No. BAC79425.1) (SEQ ID NO:40); GenBank Accession No. DQ446732.1 (SEQ ID NO:41) or NM_115529.1 (SEQ ID NO:42); and Ostrinia scapulalis (GenBank Accession No. EU817405.1) (SEQ ID NO:43).
In certain embodiments, the FAR polypeptide encompassed by the invention is coded for by a polynucleotide sequence that has been codon optimized. In particular embodiments, the polynucleotides that encode the FAR enzymes described herein are codon-optimized for expression in a host bacterial cell. Indeed, it is intended that the polynucleotides of the present invention be produced using any suitable methods and components as known in the art.
In some embodiments, a FAR enzyme is encoded by a polynucleotide sequence that has at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 93%, at least about 95%, at least about 96%, at least about 97%, or at least about 99% sequence identity to SEQ ID NO:3 SEQ ID NO:5 and/or SEQ ID NO:9 and further hybridizes with SEQ ID NO:3, SEQ ID NO:5 and/or SEQ ID NO:9 under medium, medium-high, high or very high stringency conditions.
Further Gene Manipulations
According to one embodiment of the invention, an engineered cell may further include a heterologous thioesterase (“TE”). The thioesterase may be one that preferentially uses C12, C14 or C16 ACPs. Depending on the TE used a homogenous population of fatty alcohols may be produced. For example, if the TE is one that predominantly uses C12 ACPs then the fatty alcohol composition produced by an engineered microbial cell according to the invention will predominantly comprise fatty alcohols having a carbon chain length of C12.
Some nonlimiting examples of heterologous TEs that may be used include the “class I” and “class II” acyl-ACP TE fat genes (e.g. fatA or fatB genes and reference is made to A. Jones et al., 1995, Plant Cell 7:359-371). Plant thioesterases are also described in U.S. Pat. Nos. 5,455,167 and 5,667,997). In particular, FatB are preferred TEs (e.g. plant acyl-ACP TEs) useful in the invention. In some embodiments, the TE may be a bacterial acyl-ACP TE. FatB may be obtained for example from Umbellularia california having Accession number Q41635; and AAA34215; Ulmus Americana having Accession number AAB71731, Cuphea hookeriana Accession numbers Q39513; AAC49269; AAC49269; and AAC72881 ; Cinnamonum camphorum having Accession number Q39473; AAC49151 and acyl-ACP thioesterases from Cuphea palustris (AAC49179; and U.S. Pat. No. 5,955,329). Other TEs include without limitation CnFatB ( Cocos nucifera , e.g. JF338903; JF338904 and JF338905); ccFAT ( Cinnamomum camphora ); pdFat ( Parabacteroides distasonis , ATCC 8503); gsFat ( Geobacillus sp. Y412MC10); pvFAT ( Paenibacillus vortex V453); pm FAT ( Parabacteroides merdae ATCC 43184); cvFatB ( Cuphea viscosissima , JF338906; JF338907; and JF338908); eoFat ( Elaeis oleifera ) AAD42220 ( Elaeis guineensis ) and mlFat ( Madhuca longofolia var. latifolia ).
It is known that different acyl-ACP TE have different degrees of chain length specificity. In some preferred embodiments, the TE useful in the invention is a TE having a preference for cleaving chain lengths of any one of C12, C14 and/or C16 fatty acids from ACP. In some embodiments, having a preference for cleaving chain lengths of any one of C12, C14 and/or C16 fatty acids from ACP means that the thioester hydrolysis will produce fatty acids having at least 85%, (such as at least 88%, 90%, 93%, 95%, 96%, 97%, or more) of any one of C12, C14 and/or C16 carbon chain lengths.
In one embodiment, the invention comprises an engineered bacterial cell wherein the carbon chain length of acyl-derived products (e.g., fatty acids and/or fatty alcohols) produced from the bacterial cell have been altered. In certain embodiments, the engineered cell comprises a modified native bacterial gene having β-ketoacyl-ACP synthase activity (e.g., a native β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) or a native β-ketoacyl-ACP synthase II (FabF) (EC 2.3.1.179)) and a heterologous TE. In some embodiments the TE is encoded by a gene comprising the polynucleotide sequence having at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polynucleotide sequence of SEQ ID NO:7. In some embodiments, the TE enzyme will comprise at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polypeptide sequence of SEQ ID NO:8. In some embodiments the gene encoding the TE enzyme is derived from Umbelluria californica (California Bay) and in other embodiments the gene encoding the TE enzyme is derived from Cinnamomum camphorum . In some embodiments, the TE is an E. coli TE, a variant thereof or a naturally occurring equivalent thereof that has been introduced into the engineered cell. More specifically the TE may be a TesA that is obtained from E. coli , or an analogous sequence (See, WO 2010/075483).
In some embodiments, the TE enzyme will be a functional fragment of a native TE, such as a TE having deletions at the N-terminal amino acid positions. In certain embodiments, the functional fragment will comprise at least 95% of the reference enzyme. In certain embodiments, the functional fragment will include a deletion of at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or more amino acid residues. In some embodiments, the TE is a variant enzyme having at least 1, at least 5, at least 10, at least 15 or more amino acid modifications, such as substitutions. Non-limiting examples include the TE FatB genes from Umbelluria californica (California Bay), Cinnamomum camphora , or from various Cuphea species such as those disclosed in WO 2011/008565 and reference is made to SEQ ID NOs. 21, 48, 52, 56, 60, 64, 66, 70, 72, 76, 80, 82, 86, 90, 92, 94, 96 and 100 described therein.
Further acyl-ACP TEs that are useful according to the invention are described in the following references: U.S. Pat. Nos. 5,344,771, 5,512,482; 6,150,512; 5,723,761; 5,910,631 and WO2010/075483.
Various assays are known which can be used to test for TE activity in a recombinant microorganism transformed with a vector comprising a polynucleotide encoding a TE according to the invention (See, Voelker and Davies, 1994, J. Bacteriol. 176:7320).
In certain embodiments of the invention the engineered bacterial cell comprises a) a modified native bacterial gene having β-ketoacyl-ACP synthase activity (e.g., a native β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) or a native β-ketoacyl-ACP synthase II (FabF) (EC 2.3.1.179) wherein the native β-ketoacyl-ACP synthase has at least 85% (at least 88%, at least 90%, at least 92%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%) amino acid sequence identity to SEQ ID NO:2 and comprises an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2 and b) a heterologous polynucleotide sequence encoding a thioesterase. In some embodiments, the TE is encoded by a gene comprising the polynucleotide sequence having at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polynucleotide sequence of SEQ ID NO:7. In some embodiments, the TE enzyme will comprise at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polypeptide sequence of SEQ ID NO:8. In some embodiments the gene encoding the TE enzyme is derived from Umbelluria californica (California Bay) and in other embodiments the gene encoding the TE enzyme is derived from Cinnamomum camphorum.
In certain embodiments, the engineered bacterial cell comprises a) a modified native bacterial gene having β-ketoacyl-ACP synthase activity (e.g., a native β-ketoacyl-ACP synthase I (FabB) (EC 2.3.1.41) or a native β-ketoacyl-ACP synthase II (FabF) (EC 2.3.1.179) wherein the native β-ketoacyl-ACP synthase has at least 85% (at least 88%, at least 90%, at least 92%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%) amino acid sequence identity to SEQ ID NO:2 and comprises an amino acid substitution at one or more of the following positions K40, S42, R45, G106, G107, G108, P110, Q113, A117, V133, V134, A137, M138, M197, E200, F201, A206, E216, K217, I231, T300, T302, P303, G305, L335, or Q367 when optimally aligned with SEQ ID NO:2.
In some embodiments, the TE is encoded by a gene comprising the polynucleotide sequence having at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polynucleotide sequence of SEQ ID NO:7. In some embodiments, the TE enzyme will comprise at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polypeptide sequence of SEQ ID NO:8. In some embodiments the gene encoding the TE enzyme is derived from Umbelluria californica (California Bay) and in other embodiments the gene encoding the TE enzyme is derived from Cinnamomum camphorum.
According to an embodiment of the invention, the engineered bacterial cell comprises, in addition to the modified native bacterial gene having β-ketoacyl-ACP synthase activity (e.g., a native β-ketoacyl-ACP synthase 1 (FabB) (EC 2.3.1.41) or a native β-ketoacyl-ACP synthase II (FabF) (EC 2.3.1.179) and a heterologous polynucleotide encoding a TE, a heterologous polynucleotide encoding a FadD. In some embodiments, the fadD includes the E. coli fadD (Black et al., 1992. J. Biol. Chem. 267: 25513-25520). In some embodiments, the fadD gene will comprise at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polynucleotide coding sequence illustrated in FIG. 4 of Black, supra. In some embodiments, the FadD will comprise at least 70%, (at least 75%, 80%, 85%, 90%, 93%, 95%, 97%, 99%, and even 100%) sequence identity to the polypeptide sequence illustrated in FIG. 4 of Black, supra. In some embodiments, the fadD genes include without limitation, fadD from Acinetobacter sp. NCBI ID YP_045024 (SEQ ID NO:44); fadD from Haemophilus influenza NCBI ID NP_438551 (SEQ ID NO:45); fadD from Pseudomonas aeruginosa NCBI ID NP_251989 (SEQ ID NO:46) and NP_251990 (SEQ ID NO:47); BH3101 from Bacillus halodurans NP_243969 (SEQ ID NO:48); yhfL from Bacillus subtilis NP_388908 (SEQ ID NO:49); and fadD from Rhizobium etli CFN NCBI ID_533919; fadD from Marinobacter algicola ZP_01892995 (SEQ ID NO:50); fadD from Marinobacter aquaeolei YP_958864 (SEQ ID NO:51); fadD from Mycobacterium tuberculosis NP_215722 (SEQ ID NO:52); fadD15 from Mycobacterium tuberculosis NP_216703 (SEQ ID NO:53); fadD19 from Mycobacterium tuberculosis YP_177983 (SEQ ID NO:54); fadD from Rhodopseudomonas palustris YP_00993712; fadD from Pseudomonas fluorscens PfO-1 YP_350081 (SEQ ID NO:55); fadD from Pseudomonas putida ACC77300; fadK from E. coli strain W ZP_07590374 (SEQ ID NO:56); putative fadK from Salmonella typhimurium LT2 NP_460316 (SEQ ID NO:57); and putative fadK from Thermomonospora fusca YP_290214 (SEQ ID NO:58).
In other embodiments, enzymes and genes involved in β-oxidation particularly in bacterial cells (e.g., E. coli ) such as acyl-CoA synthase (EC 6.2.1.-) and acyl-CoA dehydrogenase (FadE) (EC 1.3.99.3) may be overexpressed or attentuated. In certain embodiments fab genes other than fabB may be manipulated. Non-limiting examples include fabF (EC2.3.1.179); fabG (EC 1.1.1.100); fabA (EC4.2.1.60), fabH (EC2.3.1.180), and/or fabI (EC 1.3.19).
Culturing and Fermentation
Any suitable means for culturing the recombinant host cells finds use in the present invention. Indeed, any suitable fermentation protocol finds use in the production of the fatty alcohols provided herein. In some embodiments, fermentation of the engineered cells as described hereinabove comprises fermenting said cells which comprise a) a polynucleotide encoding a variant FabB comprising at least 85% (also at least 88%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%) amino acid sequence identity to SEQ ID NO:2 and an amino acid substitution at one or more of the following positions 30, 34, 38, 40, 42, 43, 45, 51, 53, 61, 62, 63, 64, 65, 66, 95, 96, 97, 106, 107, 108, 110, 111, 112, 113, 114, 115, 116, 117, 124, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 149, 151, 161, 162, 164, 165, 191, 196, 197, 200, 201, 204, 205, 206, 209, 210, 212, 216, 217, 224, 225, 226, 229, 231, 267, 268, 270, 271, 272, 273, 275, 282, 285, 298, 300, 302, 303, 304, 305, 308, 311, 314, 315, 333, 335, 336, 360, 361, 367, 390, 391, 392, or 396 when optimally aligned with SEQ ID NO:2; b) a heterologous polynucleotide encoding a FAR enzyme; c) optionally one or more heterologous polynucleotides encoding a fab enzyme such as FabA, FabI, FabF, and/or FabH and d) optionally a polynucleotide encoding a heterologous thioesterase under suitable conditions and for a time sufficient for production of fatty alcohols, as desired. Conditions for the culture and production of cells, including bacterial and yeast cells, are readily available and well-known in the art. Cell culture media in general are set forth in Atlas and Parks (eds.) The Handbook of Microbiological Media (1993) CRC Press, Boca Raton, Fla. which is incorporated herein by reference. Additional information for cell culture is found in available commercial literature such as the Life Science Research Cell Culture Catalogue (1998) from Sigma-Aldrich, Inc (St Louis, Mo.) (“Sigma-LSRCCC”) and, for example, The Plant Culture Catalogue and supplement (1997) also from Sigma-Aldrich, Inc (St Louis, Mo.) (“Sigma-PCCS”), all of which are incorporated herein by reference. Also reference is made to the Manual of Industrial Microbiology and Biotechnology. A. Demain and J. Davies Eds. ASM Press. 1999.
In some embodiments, the recombinant cells encompassed by the invention are grown under batch or continuous fermentations conditions. Classical batch fermentation is a closed system, wherein the compositions of the medium is set at the beginning of the fermentation and is not subject to artificial alterations during the fermentation. A variation of the batch system is a fed-batch fermentation which also finds use in the present invention. In this variation, the substrate is added in increments as the fermentation progresses. Fed-batch systems are useful when catabolite repression is likely to inhibit the metabolism of the cells and where it is desirable to have limited amounts of substrate in the medium. Batch and fed-batch fermentations are common and well known in the art. Continuous fermentation is a system where a defined fermentation medium is added continuously to a bioreactor and an equal amount of conditioned medium (e.g., containing the desired end-products) is removed simultaneously for processing. Continuous fermentation generally maintains the cultures at a constant high density where cells are primarily in the growth phase where production of end products is enhanced. Continuous fermentation systems strive to maintain steady state growth conditions. Methods for modulating nutrients and growth factors for continuous fermentation processes as well as techniques for maximizing the rate of product formation are well known in the art of industrial microbiology.
In some embodiments, fermentations are carried out a temperature within the range of from about 10° C. to about 60° C., from about 15° C. to about 50° C., from about 20° C. to about 45° C., from about 25° C. to about 45° C., from about 30° C. to about 45° C. and from about 25° C. to about 40° C. Indeed, it is intended that any suitable fermentation temperature will be used in the present invention.
In some other embodiments, the fermentation is carried out for a period of time within the range of from about 8 hours to 240 hours, from about 8 hours to about 168 hours, from about 16 hours to about 144 hours, from about 16 hours to about 120 hours, or from about 24 hours to about 72 hours. Indeed, it is intended that any suitable fermentation time will find use in the present invention.
In some other embodiments, the fermentation will be carried out at a pH in the range of about 4 to about 8, in the range of about 4.5 to about 7.5, in the range of about 5 to about 7, or in the range of about 5.5 to about 6.5. Indeed, it is intended that any suitable pH range will find use in the present invention.
Carbon sources useful in the aqueous fermentation medium (e.g., broth) in which the recombinant microorganisms are grown are those that can be assimilated by the recombinant host strain. Such carbon sources are available in many forms and include renewable carbon sources, including but not limited to cellulosic and starch feedstock substrates obtained therefrom. Such examples include for example fermentable sugars such as monosaccharides, disaccharides, and short chain oligosaccharides (e.g., glucose, fructose, xylose, galactose, arabinose, maltose, mannose, arabinose, and sucrose, as well as numerous other sugars; it is not intended that the present invention be limited to any particular fermentable sugar). Other carbon sources include, but are not limited to saturated and unsaturated fatty acids, glycerol, lactose, succinate, acetate and mixtures thereof.
In some embodiments, the assimilable carbon source is from cellulosic and/or starch feedstock derived from but not limited to, wood, wood pulp, paper pulp, grain (e.g., corn grain), corn stover, corn fiber, rice, paper and pulp processing waste, woody or herbaceous plants and residue, fruit or vegetable pulp, distillers grain, grasses, rice hulls, wheat straw, cotton, hemp, flax, sisal, corn cobs, sugar cane bagasse, sugar beets, sorghum, barely, barely straw, switch grass, wood chips, municipal solid wastes, aquatic crops, and mixtures thereof.
In some embodiments, the cellulosic feedstock useful as an assimilable carbon source has been derived from a biomass substrate that has been pretreated. The term “biomass” is broadly used herein to encompasses any living or dead biological material that contains a polysaccharide substrate, including but not limited to cellulose, starch, other forms of long-chain carbohydrate polymers, and mixtures of such sources. Examples of biomass include, but are not limited to, wood, wood pulp, paper pulp, corn fiber, corn grain, corn cobs, sugar cane, sugar beet, crop residues such as corn husks, corn stover, grasses, wheat, wheat straw, barley, barley straw, hay, rice, rice straw, switchgrass, waste paper, paper and pulp processing waste, woody or herbaceous plants, fruit or vegetable pulp, distillers grain, grasses, rice hulls, cotton, hemp, flax, sisal, sugar cane bagasse, sorghum, soy, switchgrass, components obtained from milling of grains, trees, branches, roots, leaves, wood chips, sawdust, shrubs and bushes, vegetables, fruits, and flowers and any suitable mixtures thereof. In some embodiments, the biomass comprises, but is not limited to cultivated crops (e.g., grasses, including C4 grasses, such as switch grass, cord grass, rye grass, miscanthus , reed canary grass, or any combination thereof), sugar processing residues, for example, but not limited to, bagasse (e.g., sugar cane bagasse, beet pulp [e.g., sugar beet], or a combination thereof), agricultural residues (e.g., soybean stover, corn stover, corn fiber, rice straw, sugar cane straw, rice, rice hulls, barley straw, corn cobs, wheat straw, canola straw, oat straw, oat hulls, corn fiber, hemp, flax, sisal, cotton, or any combination thereof), fruit pulp, vegetable pulp, distillers' grains, forestry biomass (e.g., wood, wood pulp, paper pulp, recycled wood pulp fiber, sawdust, hardwood, such as aspen wood, softwood, or a combination thereof). Furthermore, in some embodiments, the biomass comprises cellulosic waste material and/or forestry waste materials, including but not limited to, paper and pulp processing waste, municipal paper waste, newsprint, cardboard and the like. In some embodiments, biomass comprises one species of fiber, while in some alternative embodiments the biomass comprises a mixture of fibers that originate from different biomasses. In some embodiments, the biomass may also comprise transgenic plants that express ligninase and/or cellulase enzymes (See e.g., US 2008/0104724 A1).
In some embodiments, the biomass substrate is “pretreated,” using methods known in the art, such as chemical pretreatment (e.g., ammonia pretreatment, dilute acid pretreatment, dilute alkali pretreatment, or solvent exposure), physical pretreatment (e.g., steam explosion or irradiation), mechanical pretreatment (e.g., grinding or milling) and biological pretreatment (e.g., application of lignin-solubilizing microorganisms) and combinations thereof, to increase the susceptibility of cellulose to hydrolysis. In some embodiments, the substrate is slurried prior to pretreatment. The following references described various means of pretreatment. Steam explosion performing acid pretreatment of biomass substrates is described in U.S. Pat. No. 4,461,648. Continuous pretreatment using a slurry is described U.S. Pat. No. 7,754,457. Methods of alkali pretreatment is such as Ammonia Freeze Explosion, Ammonia Fiber Explosion or Ammonia Fiber Expansion (“AFEX”) are described in U.S. Pat. Nos. 5,171,592; 5,037,663; 4,600,590; 6,106,888; 4,356,196; 5,939,544; 6,176,176; 5,037,663 and 5,171,592. Alternative methods to AFEX utilizing a dilute ammonia pretreatments are described in WO2009/045651 and US 2007/0031953. Chemical pretreatments with organic solvents are disclosed in U.S. Pat. No. 4,556,430. Other pretreatments methods are disclosed in U.S. Pat. No. 7,465,791, and Weil et al. (1997) Appl. Biochem. Biotechnol., 68(1-2): 21-40 [1997].
Production of Fatty Alcohols
In various embodiments, fatty alcohols produced by the methods of the invention are further recovered or isolated. Recovery or isolation of the produced fatty alcohols refers to substantially separating the fatty alcohols from other components of the culture medium or fermentation process. Recovery or isolation may be accomplished by solvent extraction of the aqueous nutrient medium with a suitable water immiscible solvent. Extraction may occur simultaneously with fatty alcohol production and in some embodiments, extraction is continuous. Phase separation followed by solvent removal provides the fatty alcohol which may then be further purified and fractionated using methods and equipment known in the art. In some other aspects of the invention, the fatty alcohols in the extracellulase environment coalesce to form a water immiscible phase that can be directly separated from the aqueous nutrient medium either during the fermentation process or after its completion.
In certain embodiments of the invention, at least about 10%, at least about 20%, at least about 25%, at least about 30%, at least about 35%, at least about 40%, at least about 45%, at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, or at least about 95%, of the fatty alcohols produced by the methods described herein are secreted into the extracellular environment by the recombinant host cells.
In certain embodiments, fatty alcohols are isolated by separating the host cells from the aqueous nutrient medium, for example by centrifugation, resuspension and extraction of the fatty alcohols from the recombinant host cells using an organic solvent or solvent mixture. Suitable protocols for recovering fatty alcohols from recombinant host cells and/or culture medium are known to the skilled artisan. In some embodiments, fatty alcohols may be recovered by first lysing the cells to release the fatty alcohols and then extracting the fatty alcohol from the lysate using conventional means. Reference is also made to Yeast Protocols Handbook, (2009) Clontech Laboratories, Inc. A Takara Bio Company, Mt. View Calif. 94043; PNAS 2003 Vol. 100, 16:9156-9161; and Doan et al., (2009) J. Plant Physiol. 166: 787-796 which discloses methods to isolate and measure fatty alcohols produced in E. coli using FARs from Arabidopsis . Indeed, it is intended that any suitable method will find use in the present invention and it is not intended that the present invention be limited to any particular method(s) for separating host cells from the culture or nutrient medium.
In various embodiments, the compositions produced by the methods and microorganisms described herein comprise both saturated and unsaturated fatty alcohols. In certain embodiments, the unsaturated fatty alcohols are mono-unsaturated fatty alcohols. In some embodiments, the fatty alcohol compositions comprise both saturated and unsaturated fatty alcohols, and the amount of unsaturated fatty alcohols compared to saturated fatty alcohols in the total fatty alcohol composition is less than about 40%, less than about 35%, less than about 30%, less than about 20%, less than about 15%, less than about 10%, less than about 5%, or less than about 1% of the fatty alcohols present in the composition.
In some embodiments, the percentage of saturated fatty alcohols in the fatty alcohol compositions produced by the engineered bacterial cells encompassed by the invention is greater than about 50%, greater than about 55%, greater than about 60%, greater than about 65%, greater than about 70%, greater than about 75%, greater than about 80%, greater than about 85%, greater than about 90%, greater than about 95%, or greater than about 97%.
In some embodiments, the fatty alcohol compositions produced by the methods and engineered microorganisms described herein comprise one or more fatty alcohols selected from 1-decanol (C10:0), 1-dodecanol (C12:0), 1-tetradecanol (C14:0), 1-hexadecanol (C16:0), and 1-octadecanol (C18:0).
In some typical embodiments, C10 to C16 fatty alcohols comprise at least about 90%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% by weight of the total fatty alcohols produced by the recombinant host cells. In some embodiments, C12 to C16 fatty alcohols comprise at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, or at least about 98% by weight of the total fatty alcohols produced by the recombinant host cells.
In certain embodiments, C14 to C16 fatty alcohols comprise at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at leas, about 90%, at least about 92%, at least about 95%, at least about 97%, or at least about 99% by weight of the total produced fatty alcohols.
In certain embodiments, C12 to C14 fatty alcohols comprise at least about 50%, at least 55%, at least 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 95%, at least about 97%, or at least about 99% by weight of the total produced fatty alcohols.
In some embodiments, C12:0 to C16:0 fatty alcohols comprise at least about 75%, at least about 80% at least about 85%, at least about 90%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, or at least about 98% by weight of the total produced fatty alcohols. In certain embodiments, C14:0 to C16:0 fatty alcohols comprise at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 95%, at least about 97%, or at least about 99% by weight of the total produced fatty alcohols.
In certain embodiments, C12:0 to C14:0 fatty alcohols comprise at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 95%, at least about 97%, or at least about 99% by weight of the total produced fatty alcohols.
In certain embodiments, the C12 carbon chain length fatty alcohols produced by a recombinant bacterial microorganism according to the invention is increased by at least 10% (such as at least 15%, at least 20%, at least 25%, at least 30%, at least 35%, at least 40% or more) as compared to the production of C12 carbon chain fatty alcohols produced by a corresponding bacterial microorganism comprising a native bacterial gene when grown under essentially the same conditions.
The proportions of saturated and unsaturated fatty alcohols produced by the strains may be calculated after quantifying all the fatty alcohol species using any suitable method known in the art (e.g., GC-FID as described in US 2011/0000125SA1). The saturated fraction represents the sum of all C12:0-OH; C14:0-OH; C16:0-OH and C18:0-OH. While the unsaturated fraction is composed of the sum of C12:1-OH; C14:1-OH; C16:1-OH and C18:1-OH.
In some embodiments, the fatty alcohol compositions produced by the recombinant cells comprise a % of saturated fatty alcohols that is greater than about 55%; greater than about 60%; greater than about 65%; greater than about 70%; greater than about 75%; greater than about 80%; greater than about 85%; greater than about 90%; greater than about 95%; or greater than about 97%. In some additional embodiments, the fatty alcohol compositions further comprise at least about 85%, at least about 88%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, or at least about 98% C12 to C16 fatty alcohols. In some additional embodiments, the fatty alcohol compositions further comprise at least about 85%, at least about 88%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%, at least about 96%, at least about 97%, or at least about 98% C12 to C14 fatty alcohols.
In certain embodiments, the amount of fatty alcohols produced by the recombinant bacterial cells according to the methods described herein comprise saturated and/or unsaturated C10 to C16 alcohols in a range of about 10 mg/L to about 150 g/L of aqueous nutrient medium, such as in a range of about 10 mg/L to about 125 g/L, about 10 mg/L to about 100 g/L, about 10 mg/L to about 75 g/L, about 10 mg/L to about 50 g/L, about 10 mg/L to about 25 g/L, about 10 mg/L to about 5 g/L or in a range of about 10 mg/L to about 2 g/L of medium, using routine modification of culturing conditions. In some embodiments, the amount of fatty alcohols produced by the methods described herein is at least about 0.5 g/L, at least about 1 g/L, at least about 1.5 g/L, at least about 2.0 g/L, at least about 2.5 g/L, at least about 3 g/L, at least about 3.5 g/L, at least about 4 g/L, at least about 4.5 g/L, at least about 5 g/L, or at least about 10 g/L of medium. In various embodiments, the amount of fatty alcohols produced by the methods described herein is at least about 20 g/L, at least about 30 g/L, at least about 40 g/L, or at least about 50 g/L of medium.
In some embodiments, a recombinant bacteria (e.g., E. coli ) encompassed by the invention produces C12 to C16 fatty alcohols in an amount of at least about 1.0 g/L, at least about 5.0 g/L, at least about 10 g/L, at least about 15 g/L, at least about 20 g/L, at least about 25 g/L, or at least about 30 g/L of medium. One method to extract and quantify fatty alcohols is provided in US Patent Application 2011/0000125. However, it is not intended that the present invention be limited to any particular method(s) for extracting and/or quantifying the fatty alcohols produced using the present invention, as any suitable methods find use.
In some embodiments, the amount of fatty alcohols produced by the methods described herein are in at least about 100 mg/g, at least 500 mg/g, at least 1 g/g, at least 2 g/g, at least 5 g/g/ at least 6 g/g, at least 7 g/g, at least 8 g/g/ at least 9 g/g/ at least 10 g/g/ at least 12 g/g at least 15 g/g of dry cell weight. In some embodiments the amount of fatty alcohols produced by the methods described herein are in the range of about 100 mg/g to about 15 g/g of dry cell weight and also in the range of about 100 mg/g to about 10 g/g of dry cell weight. In other embodiments, the amount of fatty alcohols produced by the methods described herein is in the range of about 1 g/g to about 12 g/g; about 1 g/g to about 10 g/g; about 1 g/g to about 5 g/g of dry cell weight, and about 5 g/g to about 10 g/g of dry cell weight.
In certain embodiments, the amount of fatty alcohols produced by the methods described herein is in the range of about 10% to about 20% of dry cell weight, about 20% to about 30% of dry cell weight, about 30% to about 40% of dry cell weight, about 40% to about 50% of dry cell weight, about 50% to about 60% of dry cell weight, about 60% to about 70% of dry cell weight, or about 70% to about 80% of dry cell weight.
In some embodiments, the fatty alcohol compositions produced by the engineered cells and methods described herein may also comprise fatty acid-derived compounds. Fatty acid derivative compounds include compounds such as but not limited to esters (e.g. acetyl, methyl or ethyl esters and waxes) and fatty acids.
Uses of the Biosynthetically Produced Fatty Alcohols
In yet another aspect, the present invention relates to the use of the engineered organisms as described herein for the production of various compositions comprising the produced fatty alcohols, including but not limited to, fuel compositions (e.g., biodiesels and petrodiesels), cleaning compositions including detergent compositions (e.g., laundry detergents in liquid gel, spray, and powder form, hard surface cleaners, dishwashing detergents, and the like); industrial compositions (e.g., lubricants, solvents, and industrial cleaners); and personal care compositions (e.g., soaps, cosmetics, shampoos, gels, etc.).
In some embodiments, the fatty alcohol compositions described herein, and compounds derived there from, can be used as components of detergent compositions. Detergent compositions comprising fatty alcohols and fatty alcohol derivatives produced by the methods of the present invention include compositions used in cleaning applications, including, but not limited to, laundry detergents, hand-washing agents, dishwashing detergents, rinse-aid detergents, household detergents, and household cleaners, in liquid, gel, granular, powder, or tablet form. In some embodiments, the fatty alcohols produced by the methods described above and fatty alcohol derivatives are used directly in detergent compositions. In some embodiments, the fatty alcohols and fatty alcohol derivatives are reacted with a sulfonic acid group to produce sulfate derivatives that can be used as components of detergent compositions. In some embodiments, the fatty alcohols and fatty alcohol derivatives are reacted with an ethylene oxide to produce ethoxylated derivatives that can be used as components of detergent alcohols. Detergent compositions that can be generated using the fatty alcohols and fatty alcohol derivatives produced by the methods of the present invention include, but are not limited to, hair shampoos, rinses, and conditioners for humans and other animals, carpet shampoos, hard surface cleaners, light-duty household cleaners, light-duty household detergents, heavy-duty household cleaners, and heavy-duty household detergents. Detergent compositions generally include, in addition to fatty alcohols and derivative thereof, one or more builders (e.g., sodium carbonate, complexation agents, soap, and zeolites), enzymes (e.g., proteases, lipases, cellulases, and/or amylases); carboxymethyl cellulose, optical brighteners, fabric softeners, colourants and perfumes (e.g., cyclohexyl salicylate). Indeed, it is not intended that the present invention be limited to any particular detergent, detergent formulation, nor detergent use.
In some embodiments, sulfate derivatives and/or ethoxylated derivatives (e.g., C12-15) derived from fatty alcohols are used in products such as hair shampoos, carpet shampoos, light-duty household cleaners, and light-duty household detergents. In some embodiments, sulfate derivatives and/or ethoxylated derivatives (e.g., C16-C18) derived from fatty alcohols are used in products such as hair shampoos and conditioners. In some embodiments, sulfate derivatives and/or ethoxylated derivatives (e.g., C16-18) derived from fatty alcohols are used in products such as heavy-duty household cleaners and heavy-duty household detergents. Indeed, it is not intended that the present invention be limited to any particular detergent, detergent formulation, nor detergent use.
In some embodiments, fatty alcohol compositions as described herein, and compounds derived there from, are used as components in personal care compositions. In some embodiments, the fatty alcohols produced by the methods described above are used directly in personal care compositions. Personal care compositions containing fatty alcohols or fatty alcohol derivatives produced by the methods of the present invention include compositions used for application to the body (e.g., for application to the skin, hair, nails, or oral cavity) for the purposes of grooming, cleaning, beautifying, or caring for the body, including but not limited to lotions, balms, creams, gels, serums, cleansers, toners, masks, sunscreens, soaps, shampoos, conditioners, body washes, styling aids, and cosmetic compositions (e.g., makeup in liquid, cream, solid, anhydrous, or pencil form).
In some embodiments, the fatty alcohols or fatty alcohol derivatives can be reacted with a sulfonic acid group to produce sulfate derivatives that can be used as components of said compositions. In some embodiments, the fatty alcohols and fatty alcohol derivatives are reacted with an ethylene oxide to produce ethoxylated derivatives that can be used as components of said compositions. In some embodiments, sulfate derivatives (e.g., C12 to 14) derived from the fatty alcohol compositions produced by the methods described herein are used in products such as toothpastes. Indeed, it is not intended that the present invention be limited to any particular formulation, nor use.
In some embodiments, fatty alcohol compositions (e.g., C12) produced by the methods described herein are used in products such as lubricating oils, pharmaceuticals, and as an emollient in cosmetics. In some embodiments, fatty alcohol compositions (e.g., C14) produced by the methods described herein are used in products such as cosmetics (e.g., cold creams) for its emollient properties. In some embodiments, fatty alcohol compositions (e.g., C16) produced by the methods described herein are used in products such as cosmetics (e.g., skin creams and lotions) as an emollient, emulsifier, or thickening agent. In some embodiments, fatty alcohol compositions (e.g., C18) produced by the methods described herein are used in products such as lubricants, resins, perfumes, and cosmetics (e.g., as an emollient, emulsifier, or thickening agent). Indeed, it is not intended that the present invention be limited to any particular formulation, nor use.
In some embodiments, fatty alcohol compositions (e.g., C12) produced by the methods described herein are used in products such as lubricating oils, pharmaceuticals, and as an emollient in cosmetics. In some embodiments, fatty alcohol compositions (e.g., C14) produced by the methods described herein are used in products such as cosmetics (e.g., cold creams) for its emollient properties. In some embodiments, fatty alcohol compositions (e.g., C16) produced by the methods described herein are used in products such as cosmetics (e.g., skin creams and lotions) as an emollient, emulsifier, or thickening agent. In some embodiments, fatty alcohol compositions produced by the methods described herein are used in products such as lubricants, resins, perfumes, and cosmetics, e.g., as an emollient, emulsifier, or thickening agent. In some embodiments, sulfate derivatives (e.g., C12 to C14) derived from the fatty alcohol compositions produced by the methods described herein are used in products such as toothpastes.
In some instances, fatty alcohols (especially cetyl alcohol, stearyl alcohol and myristyl alcohol) may be used as food additives (e.g., adjuvants and production aids).
In some embodiments, fatty alcohols produced according to the methods described herein can be reduced to yield alkanes and/or alkenes having the same carbon chain length as the fatty alcohol starting materials. Without being bound by any particular theory, the hydroxyl group of an alcohol is a poor leaving group, and therefore, in principle a chemical moiety that binds to the oxygen atom of the hydroxyl group to make it a better leaving group can be used to reduce the fatty alcohols described herein.
Any suitable method known in the art can be used to reduce the fatty alcohols. In some embodiments, reduction of fatty alcohols is carried out chemically, for example, by a Barton deoxygenation (or Barton-McCombie deoxygenation), a two-step reaction in which the alcohol is first converted to a methyl xanthate or thioimidazoyl carbamate, and the xanthate or thioimidazoyl carbamate is reduced with a tin hydride or trialkylsilane reagent under radical conditions to produce the alkane and/or alkene. See Li et al., 2007 , Modern Organic Synthesis in the Laboratory , p. 81-83. In another embodiment, alkanes are produced by hydrogenation of fatty alcohols.
The alkanes can be isolated from the reaction mixture (which may contain unreduced fatty alcohols) to yield a composition comprising substantially all alkanes Alternatively, the alkanes and un-reduced fatty alcohols can be isolated from the reaction mixture to yield a composition comprising alkanes and fatty alcohols. In some embodiments, the fatty alcohol compositions comprise at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 85%, at least about 90%, at least about 92%, at least about 95%, at least about 96%, at least about 97%, at least about 98%, or at least about 99% alkanes by weight of the composition after reduction. In some embodiments, the alkane is octane, decane, dodecane, tetradecane, hexadecane, octadecane, icosane, docosane, tetracosane, or mixtures thereof.
In other embodiments, fatty alcohols are reacted with a carboxylic acid to form acid esters. Esterification reactions of fatty alcohols are well-known in the art. In certain embodiments, the transesterification reaction is carried out in the presence of a strong catalyst (e.g., a strong alkaline such as sodium hydroxide). In other embodiments, the esterification reaction is carried out enzymatically, using an enzyme that catalyzes the conversion of fatty alcohols to acid esters, such as lipoprotein lipase (See e.g., Tsujita et al., 1999, “Fatty Acid Alcohol Ester-Synthesizing Activity of Lipoprotein Lipase” J. Biochem. 126:1074-1079).
EXAMPLES
The present invention is described in further detail in the following Examples, which are not in any way intended to limit the scope of the invention as claimed. In the experimental disclosure below, the following abbreviations apply: M (molar); mM (millimolar), uM and μM (micromolar); nM (nanomolar); mol (moles); gm and g (gram); mg (milligrams); ug and μg (micrograms); L and l (liter); ml and mL (milliliter); cm (centimeters); mm (millimeters); um and μm (micrometers); sec. (seconds); min(s) (minute(s)); h(s) (hour(s)); U (units); LB (Luria-Bertani); MW (molecular weight); rpm (rotations per minute); ° C. (degrees Centigrade); wt % (weight percent); Δ (deletion); DNA (deoxyribonucleic acid); PCR (polymerase chain reaction); _F (forward primer); _R (reverse primer); (RNA (ribonucleic acid); gDNA (genomic DNA); cDNA (complementary DNA); PCR (polymerase chain reaction); rbs (ribosome binding sequence); Sigma (Sigma Aldrich, St. Louis, Mo.); Qiagen (Qiagen, Inc., Valencia, Calif.); Invitrogen (Invitrogen, Corp., Carlsbad, Calif.); and Promega (Promega, Corp., Madison, Wis.).
The following sequences find use in the present invention.
Native E. coli fabB DNA Sequence:
(SEQ ID NO: 1) ATGAAACGTGCAGTGATTACTGGCCTGGGCATTGTTTCCAGCATCGGTAA TAACCAGCAGGAAGTCCTGGCATCTCTGCGTGAAGGACGTTCAGGGATCA CTTTCTCTCAGGAGCTGAAGGATTCCGGCATGCGTAGCCACGTCTGGGGC AACGTAAAACTGGATACCACTGGCCTCATTGACCGCAAAGTTGTGCGCTT TATGAGCGACGCATCCATTTATGCATTCCTTTCTATGGAGCAGGCAATCG CTGATGCGGGCCTCTCTCCGGAAGCTTACCAGAATAACCCGCGCGTTGGC CTGATTGCAGGTTCCGGCGGCGGCTCCCCGCGTTTCCAGGTGTTCGGCGC TGACGCAATGCGCGGCCCGCGCGGCCTGAAAGCGGTTGGCCCGTATGTGG TCACCAAAGCGATGGCATCCGGCGTTTCTGCCTGCCTCGCCACCCCGTTT AAAATTCATGGCGTTAACTACTCCATCAGCTCCGCGTGTGCGACTTCCGC ACACTGTATCGGTAACGCAGTAGAGCAGATCCAACTGGGCAAACAGGACA TCGTGTTTGCTGGCGGCGGCGAAGAGCTGTGCTGGGAAATGGCTTGCGAA TTCGACGCAATGGGTGCGCTGTCTACTAAATACAACGACACCCCGGAAAA AGCCTCCCGTACTTACGACGCTCACCGTGACGGTTTCGTTATCGCTGGCG GCGGCGGTATGGTAGTGGTTGAAGAGCTGGAACACGCGCTGGCGCGTGGT GCTCACATCTATGCTGAAATCGTTGGCTACGGCGCAACCTCTGATGGTGC AGACATGGTTGCTCCGTCTGGCGAAGGCGCAGTACGCTGCATGAAGATGG CGATGCATGGCGTTGATACCCCAATCGATTACCTGAACTCCCACGGTACT TCGACTCCGGTTGGCGACGTGAAAGAGCTGGCAGCTATCcGTGAAGTGTT CGGCGATAAGAGCCCGGCGATTTCTGCAACCAAAGCCATGACCGGTCACT CTCTGGGCGCTGCTGGCGTACAGGAAGCTATCTACTCTCTGCTGATGCTG GAACACGGCTTTATCGCCCCGAGCATCAACATTGAAGAGCTGGACGAGCA GGCTGCGGGTCTGAACATCGTGACCGAAACGACCGATCGCGAACTGACCA CCGTTATGTCTAACAGCTTCGGCTTCGGCGGCACCAACGCCACGCTGGTA ATGCGCAAGCTGAAAGATTAA
Native E. coli FabB Amino Acid Sequence:

(SEQ ID NO: 2) MKRAVITGLGYVSSIGNNQQEVLASLREGRSGITFSQELKDSGMRSHVWG NVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVG LIAGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPF KIHGVNYSISSACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACE FDAMGALSTKYNDTPEKASRTYDAHRDGFVIAGGGGMVVVEELEHALARG AHIYAEIVGYGATSDGADMVAPSGEGAVRCMKMAMHGVDTPIDYLNSHGT STPVGDVKELAAIREVFGDKSPAISATKAMTGHSLGAAGVQEAIYSLLML EHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGFGGTNATLV MRKLKD
Polynucleotide Sequence of a Codon Optimized FAR from Marinobacter algicola DG893:

(SEQ ID NO: 3) ATGGCTACTCAACAACAACAGAACGGTGCATCTGCATCCGGCGTCTTGGA ACAACTTCGTGGAAAGCACGTTCTTATCACAGGTACTACCGGATTTTTGG GCAAAGTGGTTCTGGAAAAGTTGATTCGTACTGTTFCCGGATATTGGAGG TATTCATCTGCTGATTCGTGGCAATAAACGTCATCCAGCCGCTCGTGAAC GTTTCCTGAACGAAATTGCGTCCTCCTCCGTCTTCGAACGTTTGCGTCAC GATGATAATGAAGCCTTCGAGACCTTCTTGGAAGAACGTGTTCACTGTAT TACCGGTGAGGTTACTGAATCCCGTTTTGGTTTGACACCTGAACGTTTTC GTGCTTTGGCCGGTCAGGTTGACGCTTTTATTAACAGCGCTGCAAGCGTG AACTTTCGTGAGGAATTGGATAAAGCCCTGAAAATCAACACCTTGTGTCT TGAAAATGTTGCTGCTCTTGCAGAATTGAACTCCGCTATGGCGGTCATTC AGGTTTCCACTTGTTACGTTAACGGTAAAAACTCCGGTCAAATTACCGAA TCCGTCATTAAACCTGCTGGCGAATCCATTCCCCGTTCCACTGACGGTTA CTACGAGATCGAAGAATTGGTCCATCTGTTGCAAGACAAGATTTCCGATG TTAAAGCTCGTTACTCCGGCAAAGTTCTGGAGAAAAAATTGGTTGATTTG GGTATTCGTGAGGCCAATAATTACGGATGGTCCGACACCTACACATTCAC CAAATGGTTGGGTGAACAACTGCTGATGAAGGCCTTGTCTGGTCGTTCTT TGACTATTGTGCGTCCCTCTATTATTGAGTCCGCTTTGGAAGAACCTTCC CCTGGTTGGATCGAAGGCGTTAAAGTTGCCGATGCCATTATCTTGGCTTA TGCCCGTGAAAAAGTTAGCCTGTTCCCTGGAAAACGTTCCGGCATTATTG ATGTTATTCCTGTCGATTTGGTTGCGAACTCCATCATCTTGTCTCTGGCT GAGGCGTTGTCTGGTTCTGGTCAACGTCGTATTTATCAATGTTGCAGCGG TGGTTCTAATCCAATCTCCCTGGGTAAGTTCATTGATTATTTGATGGCCG AGGCTAAGACCAACTATGCTGCCTACGATCAACTGTTTTATCGTCGTCCT ACTAAACCTTTCGTCGCCGTGAACCGTAAATTGTTTGACGTTGTTGTTGG TGGTATGCGTGTTCCTCTTTCTATTGCCGGTAAAGCTATGCGTTTGGCTG GTCAAAATCGTGAGTTGAAAGTGCTTAAGAACCTTGATACGACCCGTTCC CTTGCAACCATTTTTGGCTTCTATACTGCTCCCGACTATATCTTCCGTAA CGATAGCTTGATGGCCCTGGCTTCTCGTATGGGTGAATTGGATCGTGTTC TTTTCCGAGTTGATGCTCGTCAAATTGATTGGCAGTTGTACTTGTGTAAA ATTCATTTGGGTGGTCTGAACCGTTACGCTTTGAAGGAACGTAAACTGTA TTCTTTGCGTGCTGCTGATACTCGTAAAAAAGCTGCCTAA
FAR Polypeptide Sequence Encoded by the Polynucleotide of SEQ ID NO: 3:

(SEQ ID NO: 4) MATQQQQNGASASGVLEQLRGKHVLITGTTGFLGKVVLEKLIRTVPDIGG IHLLIRGNKRHPAARERFLNEIASSSVFERLRHDDNEAFETFLEERVHCI TGEVTESRFGLTPERFRALAGQVDAFINSAASVNFREELDKALKINTLCL ENVAALAELNSAMAVIQVSTCYVNGKNSGQITESVIKPAGESIPRSTDGY YEIEELVHLLQDKISDVKARYSGKVLEKKLVDLGIREANNYGWSDTYTFT KWLGEQLLMKALSGRSLTIVRPSIIESALEEPSPGWIEGVKVADAIILAY AREKVSLFPGKRSGIIDVIPVDLVANSIILSLAEALSGSGQRRIYQCCSG GSNPISLGKFIDYLMAEAKTNYAAYDQLFYRRPTKPFVAVNRKLFDVVVG GMRVPLSIAGKAMRLAGQNRELKVLKNLDTTRSLATIFGFYTAPDYIFRN DSLMALASRMGELDRVLFPVDARQIDWQLYLCKIHLGGLNRYALKERKLY SLRAADTRKKAA
Polynucleotide Sequence Encoding a Variant FAR (V1):

(SEQ ID NO: 5) ATGGCGACTCAACAACAGAACAACGGTGCATCTGCATCCGGCGTCTTGGA AATTCTTCGTGGAAAGCACGTTCTTATCACAGGTACTACCGGATTTTTGG GCAAAGTGGTTCTGGAAAAGTTGATTCGTACTGTTCCGGATATTGGAGGT ATTCATCTGCTGATTCGTGGCAATAAACGTCATCCAGCCGCTCGCGAACG TTTCCTGAACGAAATTGCGTCCTCCTCCGTCITCGAACGTTTGCGTCACG ATGATAATGAAGCCTTCGAGACCTTCTTGGAAGAACGTGTTCACTGTATT ACCGGTGAGATTACTGAATCCCGTTTTGGTTTGACACCTGAGCGTTTTCG TGCTTTGGCCGGTCAGGTTGACGCTTTTATTCATAGCGCTGCAAGCGTGA ACTTTCGTGAGCAATTGGATAAAGCCCTGAAAATCAACACCTTGTGTCTT GAAAATGTTGCTGCACTTGCAGAATTGAACTCCGCTATGGCGGTCATTCA GGTTTCCACTTGTTACGTTAACGGTAAAACCTCCGGTCAAATTACCGAAT CCGTCATTAAATCGGCTGGCGAATCCATTCCCCGTTCCACTGACGGTTAC TACGAGATCGAAGAATTGGTCCATCTGTTGCAAGACAAGATTTCCGATGT TAAAGCTCGTTACTCCGGCCGTGTTATGGGGAAAAAATTGGTTGATTTGG GTATTCGTGAGGCCAATAATTACGGATGGTCCGACACCTACACATTCACC AAATGGTTGGGTGAACAACTGCTGATGAAGGCCTTGTCTGGTCGTTCTTT GACTATTGTGCGTCCCTCTATTATTGAGTCCGCTTTGGAAGAACCTTCCC CTGGTTGGATCGAAGGCGTTAAAGTTGCCGATGCCATTATCTTGGCTTAT GCCCGTGAAAAAGTTAGCCTGTTCCCTGGAAAACGTTCCGGCATTATTGA TTTTATTCCFGTCGATTTGGTTGCGAACTCCATCATCTTGTCTCTGGCTG AGGCGTTGTCTGGTTCTGGTCAACGTCGTATTTATCAATGTTGCAGCGGT GGTTCTAATCCAATCTCCCTGGGTAAGTTCTTTGATTATTTGAACGCCGA GGCTAAGACCAACTATGCTGCCTACGATCAACTGTTTTATCGTCGTCCTA CTAAACCTTTCGTCGCCGTGAACCGTAAATTGTTTGACGTTGTTGTTGGT GTCATGCGTGTTGTCCTTTCTATTGCCCGCAAAGCTATGCGTTTGGCTGG TGTAAATCGTGAGTTGAAAGTGCTTAAGAACCTTGATACGACCCGTAAAC TTGCAACCATTTTTGGCTTCTATACTGCTCCCGACTATATCTTCCGTAAC GATAGCTTGATGGCCCTGGCTCAGCGTATGGGTGAATTGGATCGTGTTCT TTTCCCAGTTGATGCTCGTCAAATTGATTGGCAGTTGTACTTGTGTAAAA TTCATTFGCGTGGTCTGAACCGTTACGCTTTGAAGGGCCGTAAACTGT.A TTCTTCGCGTGCTGCTGATACTGACGATGAAACCCGCCTAA
Variant FAR (V1) Polypeptide Sequence Encoded by the Polynucleotide Sequence of SEQ ID NO: 5

(SEQ ID NO: 6) MATQQQNNGASASGVLEILRGKHVLITGTTGFLGKVVLEKLIRTVPDIGG IHLLIRGNKRHPAARERFLNEIASSSVFERLRHDDNEAFETFLEERVHCI TGEITESRFGLTPERFRALAGQVDAFIHSAASVNFREQLDKALKINTLCL ENVAALAELNSAMAVIQVSTCYVNGKTSGQITESVIKSAGESIPRSTDGY YEIEELVHLLQDKISDVKARYSGRVMGKKLVDLGIREANNYGWSDTYTFT KWLGEQLLMKALSGRSLTIVRPSIIESALEEPSPGWIEGVKVADAIILAY AREKVSLFPGKRSGIIDFIPVDLVANSIILSLAEALSGSGQRRIYQCCSG GSNPISLGKFFDYLNAEAKTNYAAYDQLFYRRPTKPFVAVNRKLFDVVVG VMRVVLSIARKAMRLAGVNRELKVLKNLDTTRKLATIFGFYTAPDYIFRN DSLMALAQRMGELDRVLFPVDARQIDWQLYLCKIHLRGLNRYALKGRKLY SSRAADTDDETA*
Polynucleotide Sequence Encoding a Thioesterase Amino Acid Sequence of SEQ ID NO: 8:

(SEQ ID NO: 7) ATGACCTTAGAGTGGAAACCAAAACCGAAATTACCTCAGCTTCTTGACGA CCACTTCGGCCTGCATGGTTTAGTATTCCGCAGAACGTTTGCCATAAGAA GCTACGAAGTAGGACCAGATCGTTCTACCTCTATACTTGCTGTGATGAAT CATATGCAGGAAGCCACGTTAAATCACGCAAAGAGCGTCGGGATCCTTGG GGACGGATTCGGCACCACATTGGAAATGAGTAAGCGGGACCTGATGTGGG TTGTTCGTCGTACCCACGTAGCGGTCGAACGGTATCCAACATGGGGCGAT ACTGTTGAAGTGGAGTGCTGGATTGGCGCTTCCGGAAACAACGGAATGCG CAGAGATTTTCTGGTGCGGGACTGTAAAACTGGGGAAATCTTAACGCGCT GTACCTCCCTGTCCGTTCTGATGAACACGCGTACCCGGAGATTAAGTACG ATTCCGGACGAAGTCCGTGGTGAAATCGGTCCCGCTTTTATTGACAACGT GGCGGTAAAAGACGACGAGATCAAAAAGTTGCAGAAATTGAACGATTCCA CAGCAGATTACATACAGGGCGGTCTTACGCCCCGTTGGAACGACTTGGAT GTGAATCAGCACGTAAATAACCTTAAATATGTGGCGTGGGTGTTCGAGAC CGTTCCCGACTCTATTTTTGAAAGTCACCACATTTCCAGCTTTACGCTGG AGTACAGACGCGAGTGTACGCGCGATTCCGTTTTACGTTCCCTCACCACG GTGTCTGGCGGATCTTCCGAAGCTGGGTTAGTGTGTGATCACTTGCTGCA ACTTGAAGGCGGAAGTGAAGTTCTTCGGGCCCGCACGGAATGGCGTCCCA AACTGACCGATTCCTTCCGCGGAATATCAGTAATTCCGGCCGAGCCGCGG GTATAA
Polypeptide Sequence Encoded by the Thioesterase Polynucleotide Sequence of SEQ ID NO: 7:

(SEQ ID NO: 8) MTLEWKPKFKLPQLLDDHFGLHGLVFRRTFAIRSYEVGPDRSTSILAVMN HMQEATLNHAKSVGILGDGFGTTLEMSKRDLMWVVRRTHVAVERYPTWGD TVEVECWIGASGNNGMRRDFLVRDCKTGEILTRCTSLSVLMNTRTRRLST IPDEVRGEIGPAFIDNVAVKDDEIKKLQKLNDSTADYIQGGLTPRWNDLD VNQHVNNLKYVAWVFETVPDSIFESHHISSFTLEYRRECTRDSVLRSLTT VSGGSSEAGLVCDHLLQLEGGSEVLRARTEWRPKLTDSFRGISVIPAEPR V
Polynucleotide Sequence Encoding a Variant FAR (V2):

(SEQ ID NO: 9) atggcgactcaacaacagcagaacggtgcatctgcatccggcgtcttgga acaacttcgtggaaagcacgttcttatcacaggtactaccggatttttgg gcaaagtggttctggaaaagttgattcgtactgttccggatattggaggt attcatctgctgattcgtggcaataaacgtcatccagccgctcgtgaacg tttcctgaacgaaattgcgtcctcctccgtcttcgaacgtttgcgtcacg atgataatgaagccttcgagaccttcttggaagaacgtgttcactgtatt accggtgaggttactgaatcccgttttggtttgacacctgagcgttttcg tgctttggccggtcaggttgacgcttttattaacagcgctgcaagcgtga gttttcgtgagcaattggataaagccctgaaaatcaacaccttgtgtctt gaaaatgttgctgctcttgcagaattgaactccgctatggcggtcattca ggtttccacttgttacgttaacggtaaaaactccggtcaaattaccgaat ccgtcattaaatcggctggcgaatccattccccgtttccactgacggtta ctacgagatcgaagaattggtccatctgttgcaagacaagatttccgatg ttaaagctcgttactccggcaaagttctggagaaaaaattggttgatttg ggtattcgtgaggccaataattacggatggtccgacacctacacattcac caaatggttgggtgaacaactgctgatgaaggccttgtctggtcgacttt gactattgtgcgtccctctattattgagtccgcttggaagaaccttcccc tggttggatcgaaggcgttaaagttgccgatgccattatcttggcttatg cccgtgaaaaagttagcctgttccctggaaaacgttccggcattattgat gttattcctgtcgatttggttgcgaactccatcatcttgtctctggctga ggcgttgtctggttctggtcaacgtcgtatttatcaatgttgcagcggtg gttctaatccaatctccctgggtaagttcattgattatttgatggccgag gctaagaccaactatgctgcctacgatcaactgttttatcgtcgtcctac taaacctttcgtcgccgtgaaccgtaaattgtttgacgttgttgttggtg gtatgcgtgttgtcctttctattgccggtaaagctatgcgtttggctggt gtaaatcgtgagttgaaagtgcttaagaaccttgatacgacccgtaaact tgcaaccatttttggcttctatactgctcccgactatatcttccgtaacg atagcttgatggccctggctcagcgtatgggtgaattggatcgtgttctt ttcccagttgatgctcgtcaaattgatlggcagttgtacttgtgtaaaat tcatttgggtggtctgaaccgttacgctttgaaggaacgtaaactgtatt cttcgcgtgctgctgatactgacgataaaaccgcctaa
FAR V2 Polypeptide Sequence Encoded by the Polynucleotide of SEQ ID NO:9:

(SEQ ID NO: 10) MATQQQQNGASASGVLEQLRGKHVLITGTTGFLGKVVLEKLIRTVPDIGG IHLLIRGNKRHPAARERFLNEIASSSVFERLRHDDNEAFETFLEERVHCI TGEVTESRFGLTPERFRALAGQVDAFINSAASVSFREQLDKALKINTLCL ENVAALAELNSAMAVIQVSTCYVNGKNSGQITESVIKSAGESIPRSTDGY YEIEELVHLLQDKISDVKARYSGKVLEKKLVDLGIREANNYGWSDTYTFT KWLGEQLLMKALSGRSLTIVRPSIIESALEEPSPGWIEGVKVADAIILAY AREKVSLFPGKRSGIIDVIPVDLVANSIILSLAEALSGSGQRRIYQCCSG GSNPISLGKFIDYLMAEAKTNYAAYDQLFYRRPTKPFVAVNRKLFDVVVG GMRVVLSIAGKAMRLAGVNRELKVLKNLDTTRKLATIFGFYTAPDYIFRN DSLMALAQRMGELDRVLFPVDARQIDWQLYLCKIHLGGLNRYALKERKLY SSRAADTDDKTA
PLS8379:






(SEQ ID NO: 11)



GGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTC



AGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCAGATCAATTCG



CGCGCGAAGGCGAAGCGGCATGCATTTACGTTGACACCATCGAATGGTGC



AAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAG



GGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCG



GTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTT



TCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTA



CATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGA



TTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTC



GCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTC



GATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATC



TTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGAC



CAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATT



TCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATG



AAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAG



CAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCG



TCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAG



CGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATG



CAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGA



TCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCG



TTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCA



TGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGG



GCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGA



AGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTG



GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAAT



GCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAAC



GCAATTAATGTAAGTTAGCGCGAATTGATCTGGTTTGACAGCTTATCATC



GACTGCACGGTGCACCAATGCTTCTGGCGTCAGGCAGCCATCGGAAGCTG



TGGTATGGCTGTGCAGGTCGTAAATCACTGCATAATTCGTGTCGCTCAAG



GCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCGACATCATAACGGTTC



TGGCAAATATTCTGAAATGAGCTGTTGACAATTAATCATCCGGCTCGTAT



AATGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCGCCG



CTGAGAAAAAGCGAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCT



GTGTGGGCACTCGACCGGAATTATCGATTAACTTTATTATTAAAAATTAA



AGAGGTATATATTAATGTATCGATTAAATAAGGAGGAATAAACCATGGAT



CCGAGCTCGAGATCTGCAGCTGGTACCATATGGGAATTCGAAGCTTTCTA



GAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCA



TCATCATCATCATTGAGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG



ATGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCG



GTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCT



GACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGT



GGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGA



AAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGT



GAACGCTCTCCTGAGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGT



GCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGAT



GCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGAGCT



TAGTAAAGCCCTCGCTAGATTTTAATGCGGATGTTGCGATTACTTCGCCA



ACTATTGCGATAACAAGAAAAAGCCAGCCTTTCATGATATATCTCCCAAT



TTGTGTAGGGCTTATTATGCACGCTTAAAAATAATAAAAGCAGACTTGAC



CTGATAGTTTGGCTGTGAGCAATTATGTGCTTAGTGCATCTAACGCTTGA



GTTAAGCCGCGCCGCGAAGCGGCGTCGGCTTGAACGAATTGTTAGACATT



ATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTGGACAAATTCT



TCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGATA



AGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCT



CCATTGCCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACT



GCGCTGTACCAAATGCGGGACAACGTAAGCACTACATTTCGCTCATCGCC



AGCCCAGTCGGGCGGCGAGTTCCATAGCGTTAAGGTTTCATTTAGCGCCT



CAAATAGATCCTGTTCAGGAACCGGATCAAAGAGTTCCTCCGCCGCTGGA



CCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAGCAAGATAGCCAG



ATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCATTGC



GCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGA



ATGATGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTC



GCTCTCTCCAGGGGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTC



GCCGCGTTGTTTCATCAAGCCTTACGGTCACCGTAACCAGCAAATCAATA



TCACTGTGTGGCTTCAGGCCGCCATCCACTGCGGAGCCGTACAAATGTAC



GGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGACGCCAACTACCTCTG



ATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATGATGTTTAAC



TTTGTTTTAGGGCGACTGCCCTGCTGCGTAACATCGTTGCTGCTCCATAA



CATCAAACATCGACCCACGGCGTAACGCGCTTGCTGCTTGGATGCCCGAG



GCATAGACTGTACCCCAAAAAAACAGTCATAACAAGCCATGAAAACCGCC



ACTGCGCCGTTACCACCGCTGCGTTCGGTCAAGGTTCTGGACCAGTTGCG



TGAGCGCATACGCTACTTGCATTACAGCTTACGAACCGAACAGGCTTATG



TCCACTGGGTTCGTGCCTTCATCCGTTTCCACGGTGTGCGTCACCCGGCA



ACCTTGGGCAGCAGCGAAGTCGAGGCATTTCTGTCCTGGCTGGCGAACGA



GCGCAAGGTTTCGGTCTCCACGCATCGTCAGGCATTGGCGGCCTTGCTGT



TCTTCTACGGCAAGGTGCTGTGCACGGATCTGCCCTGGCTTCAGGAGATC



GGAAGACCTCGGCCGTCGCGGCGCTTGCCGGTGGTGCTGACCCCGGATGA



AGTGGTTCGCATCCTCGGTTTTCTGGAAGGCGAGCATCGTTTGTTCGCCC



AGCTTCTGTATGGAACGGGCATGCGGATCAGTGAGGGTTTGCAACTGCGG



GTCAAGGATCTGGATTTCGATCACGGCACGATCATCGTGCGGGAGGGCAA



GGGCTCCAAGGATCGGGCCTTGATGTTACCCGAGAGCTTGGCACCCAGCC



TGCGCGAGCAGGGGAATTAATTCCCACGGGTTTTGCTGCCCGCAAACGGG



CTGTTCTGGTGTTGCTAGTTTGTTATCAGAATCGCAGATCCGGCTTCAGC



CGGTTTGCCGGCTGAAAGCGCTATTTCTTCCAGAATTGCCATGATTTTTT



CCCCACGGGAGGCGTCACTGGCTCCCGTGTTGTCGGCAGCTTTGATTCGA



TAAGCAGCATCGCCTGTTTCAGGCTGTCTATGTGTGACTGTTGAGCTGTA



ACAAGTTGTCTCAGGTGTTCAATTTCATGTTCTAGTTGCTTTGTTTTACT



GGTTTCACCTGTTCTATTAGGTGTTACATGCTGTTCATCTGTTACATTGT



CGATCTGTTCATGGTGAACAGCTTTGAATGCACCAAAAACTCGTAAAAGC



TCTGATGTATCTATCTTTTTTACACCGTTTTCATCTGTGCATATGGACAG



TTTTCCCTTTGATATGTAACGGTGAACAGTTGTTCTACTTTTGTTTGTTA



GTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCT



TCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGA



GCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAA



AATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTG



TAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTG



TTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTT



ACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGT



CGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTA



AATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCA



TGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAAT



CTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACG



ACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCC



AGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTC



TTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTG



TCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACA



GTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATT



TTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATA



CCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCC



ACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACT



AATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCA



ATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAA



TGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTG



CTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATT



CCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATT



TATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCC



TGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGT



CGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGC



TTAAG
























































































































































































































































Example 1
Construction of pSIM-CDX
The chloramphenicol resistance marker in the lambda-RED expression plasmid pSIM5 (Gene. 2006 September 379:109-15) was replaced with an ampicillin resistance marker to make plasmid pSIM-CDX as described below. First, the ampicillin resistance marker from pUC19 (Invitrogen, Carlsbad, Calif.) was PCR amplified with the following oligos:
BLA-Promoter-pSIM5-Mega_F:
(SEQ ID NO: 12) 5′-GGCAAGGTGTTCTGGTCGGCGCATAGCTGAGATAAATGCTTCAA TAATATTGAAAAAGGAAGAG-3′
BLA-Promoter-pSIM5-Mega_R:






(SEQ ID NO: 13)



5′-AGGCAAAGAAAACCCGGCGCTGAGGCCGGGTTACCAATGCTTAA



TCAGTGAGGCACCTA-3′














The PCR reaction was carried out using the enzyme Herculase DNA polymerase (Agilent Technologies, Inc., Santa Clara, Calif.) with an initial denaturation step at 94° C. for 2 min, followed by 25 cycles of the steps: 94° C. for 30 sec; 56° C. for 30 sec and 72° C. for 2 min. The denaturation step was followed by a final elongation step at 72° C. for 3 min. The resulting PCR product was cleaned with ExoSAP-IT (Affymetrix, Santa Clara, Calif.) and the remaining template was digested with DpnI (Promega, Madison, Wis.).
Five microliters of cleaned PCR product was added to 40 ng of plasmid pSIM5. The mixture was PCR amplified using the enzyme Phusion DNA polymerase (New England BioLabs, Ipswich, Mass.) with an initial denaturation step at 98° C. for 30 sec. followed by 40 cycles of the steps: 98° C. for 10 sec; 72 for 3 min. The denaturation step was followed by a final elongation step at 72° C. for 5 min. After the PCR reaction, the product was digested with DpnI (Promega, Madison, Wis.). This reaction was transformed into E. coli DH10B electrocompetent cells (Invitrogen, Carlsbad, Calif.) following the manufacturer's protocols. Cells were plated on LB agar plates containing 50 micrograms/ml of carbenicillin and incubated for 24 hours at 30° C. The obtained clones were verified.
Example 2
Construction of Plasmid PLS8379
A low copy vector carrying the Trc promoter was constructed. A DNA fragment containing the LACIQ gene, the Trc promoter, and the multiple cloning sites present in PtrCHIS2-B (Invitrogen, Carlsbad, Calif.) was PCR amplifies using the primers:





1920TrcM_F



(SEQ ID NO: 14)



5′-GACCTTAAAACCCTAAAGGCTTAAGGGCATCDCGCTTACAGACA

and



1920TrcM_R



(SEQ ID NO: 15)



5′-GGAGAAAATACCGCATCAGGCGCCTCAGGAGAGCGTTCACCGAC.



























The PCR reaction was carried out using the enzyme Phusion (New England BioLabs, Ipswich, Mass.) with an initial denaturation step at 98° C. for 30 sec. followed by 25 cycles of the steps: 98° C. for 10 sec; 65° C. for 15 sec and 72° C. for 15 sec. This was followed by a final elongation step at 72° C. for 5 min. The primers used for the PCR reaction carry regions of homology to plasmid pCL1920, and therefore the PCR product described above can be used as a megaprimer to amplify a defined region of pCL1920 (Lerner and Inouye (1990) NAR 18: 4631) which contains the pSC101 origin of replication and confers resistance to spectinomycin (GenBank: AB236930). This PCR reaction was carried out using the Pfu Ultra enzyme (Agilent Technologies, Santa Clara, Calif.) with an initial denaturation step at 95° C. for 2 min, followed by 16 cycles of the steps: 95° C. for 30 sec; 55° C. for 30 sec and 68° C. for 7 min. This was followed by a final elongation step at 68° C. for 7 min. The outcome of the second PCR reaction was sequence-verified and the resulting plasmid was named pLS8379 (SEQ ID NO:11).
Example 3
Construction of Plasmid pCDX11
To obtain a tightly regulated gene expression vector, the P TRC promoter present in pLS8379 was replaced with a synthetic DNA fragment containing a P TRC variant where a symmetrical Lac operator [Sadler et al., 1983, PNAS, 80: 6785-6789] was introduced upstream of the −35 region of P TRC . This promoter was synthesized as an EcoRV-NcoI DNA fragment (GeneScript, Piscataway, N.J.) (SEQ ID NO: 16 and used to replace the EcoRV-NcoI region from pLS8379 previously cut with the same restriction enzymes. A ligation reaction containing the two DNA fragments was incubated overnight at 16° C. and then transformed into E. coli Top10 electrocompetent cells (Invitrogen, Carlsbad, Calif.) following the manufacturer's protocols. Cells were plated on LB agar plates containing 100 micrograms/ml of spectinomycin. Plates were then incubated overnight at 37° C. Obtained clones were sequence verified.
EcoRV-NcoI Fragment (SEQ ID NO: 16)





(SEQ ID NO: 16)



GATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATAT



CCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCA



GCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAAT



CAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAA



TACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGG



CACGACAGGTTTCCCGACTGGAAAGCGGGCAGTAATAATTTAAATTGGTT



TGACAGCTTATCATCGACTGCACGGTGCACCAATGCTTCTGGCGTCAGGC



AGCCATCGGAAGCTGTGGTATGGCTGTGCAGGTCGTAAATCACTGCATAA



TTCGTGTCGCTCAAGGCGCACTCCCGTTCTGGATAATGTTTTTTGCGCCG



ACATAATTGTGAGCGCTCACAATTTCTGAAATGAGCTGTTGACAATTAAT



CATCCGGCTCGTATAATGTGTGGAATTGTGAGCGGATAACAATTTCACAC



AGGAAACAGCGCCGCTGAGAAAAAGCGAAGCGGCACTGCTCTTTAACAAT



TTATCAGACAATCTGTGTGGGCACTCGACCGGAATTATCGATTAACTTTA



TTATTAAAAATTAAAGGAGGAATAAACCATGG






































Example 4
Construction of Plasmid pCDX11-V2-kanR-Term
The plasmid PCDX11-V2-KanR-Term was assembled in several steps as described below. The plasmid pCDX11-V2 comprising FAR-V2 polynucleotide of SEQ ID NO:9 encoding the FAR-V2 enzyme having the amino acid sequence of SEQ ID NO: 10 was constructed as described below. A DNA fragment containing the FAR V2 gene was PCR amplified using the following primers.







NcoI_F



(SEQ ID NO: 17)





5′ TAAACCATGGCGACTCAACAACAGAACA


and





SalI_R



(SEQ ID NO: 18)





5′ CTATGTCGACTTAGGCGGTTTCATCGTCAGTATC.



























The restriction enzyme sites for NcoI and SalI were incorporated into NcoI_F and SalI_R respectively, allowing ligation into pCDX11 (See, Example 3) digested with NcoI and SalI. Ligation reactions were incubated overnight at 16° C. and then transformed into E. coli TOP10 chemically competent cells (Invitrogen, Carlsbad, Calif.) using standard techniques. Cells were plated on LB agar plates containing 100 ug/ml of spectinomycin and incubated overnight at 37° C. Obtained clones were sequence verified. The cycling conditions and reactions were applied according to the manufacturers' instructions, unless otherwise specified.
(b) The plasmid pCDX1 I-V2-kanR-Term was constructed as described below. A dsDNA kanamycin resistance cassette was PCR amplified from plasmid pKD13 (Proc Natl Acad Sci USA. 2000 Jun. 6; 97(12):6640-5.) using the following primers:





OH-Bsu36I-KanR_F:



(SEQ ID NO: 19)



5′-GTCGGTGAACGCTCTCCTGAGGATTCCGGGGATCCGTCGACC-3′



Bsu36I-KanR-term_R:



(SEQ ID NO: 20)



5′-AACCTCAGGCGAAAAAACCCCGCCGAAGCGGGGT



TTTTTGCGTGTAGGCTGGAGCTGCTT-3′.




























The reverse primer encoded a transcriptional termination sequence in addition to having homology to the kanamycin resistance cassette. The PCR reaction was carried out using the enzyme Phusion DNA polymerase (New England BioLabs, Ipswich, Mass.) with an initial denaturation step at 98° C. for 30 sec, followed by 30 cycles of the steps: 98° C. for 5 sec; 63° C. for 20 sec and 72° C. for 30 sec. The denaturation step was followed by a final elongation step at 72° C. for 5 min. After the PCR reaction, the PCR product was purified through a PCR purification column and eluted with water.
The PCR amplified kanamycin resistance cassette and the vector pCDX11-V2 were digested with the restriction enzyme Bsu361 (Fermentas, Glen Burnie, Md.) and the resulting products were ligated using Quick T4 DNA Ligase (New England BioLabs, Ipswich, Mass.) following manufacturer recommendations. The reaction was transformed into E. coli Top10 electrocompetent cells (Invitrogen, Carlsbad, Calif.) following the manufacturer's protocols. Cells were plated on LB agar plates containing 100 micrograms/ml of spectinomycin and were incubated overnight at 37° C. The obtained clones were sequence verified.
Example 5
Construction of pCDX11-V1
The plasmid pCDX11-V1 comprising the FAR-V1 polynucleotide of SEQ ID NO:5 encoding the FAR-V1 enzyme having the amino acid sequence of SEQ ID NO:6 was constructed as described below.
A DNA fragment containing the FAR-V1 gene was PCR amplified using the primers:
NcoI_F (SEQ ID NO: 17) 5′ TAAACCATGGCGACTCAACAACAGAACA and SalI_R (SEQ ID NO: 18) 5′ CTATGTCGACTTAGGCGGTTTCATCGTCAGTATC.
The restriction enzyme sites for NcoI and SalI were incorporated into NcoI_F and SalI_R respectively, allowing ligation into pCDX11 digested with NcoI and SalI. Ligation reactions were incubated overnight at 16° C. and then transformed into E. coli TOP10 chemically competent cells (Invitrogen, Carlsbad, Calif.) using standard techniques. Cells were plated on LB agar plates containing 100 ug/ml of spectinomycin and incubated overnight at 37° C. Obtained clones were sequence verified. The cycling conditions and reactions were applied according to the manufacturers' instructions.

Example 6
Generation of FabB Variants
A library of fabB variants differing from the wild-type sequence of SEQ ID NO:2 by one amino acid was generated using synthetic oligonucleotides, employing methods described in patent application US2009057507. All possible amino acid substitutions were made at the following 96 positions within the fabB protein of SEQ ID NO:2: R30, T34, E38, K40, S42, G43, R45, N51, K53, D61, R62, K63, V64, V65, R66, N95, N96, P97, G106, G107, G108, P110, R111, F112, Q113, V114, F115, G116, A117, R124, K127, A128, G130, P131, Y132, V133, V134, T135, K136, A137, M138, A139, S140, P149, K151, S161, A162, A164, T165, E191, E196, M197, E200, F201, M204, G205, A206, T209, K210, N212, E216, K217, A224, H225, R226, F229, I1231, A267, D268, V270, A271, P272, S273, E275, K282, M285, H298, T300, T302, P303, V304, G305, K308, A311, R314, E315, H333, L335, G336, N360, I361, Q367, F390, G391, F392, and N396.
Example 7
Replacement of Native fabB
The native fabB gene in E. coli W3110K was replaced with a cassette encoding a library of fabB variants in several steps as described below:
(a) A dsDNA cassette encoding a kanamycin resistance gene, the fabB library of variants, and a chloramphenicol resistance gene was assembled. First, the kanamycin resistance cassette was PCR amplified from the plasmid pCDX11-V2-kanR-Term (See, Example 4) using the following primers:

Ultra_homolFabB5′KanFwd:


(SEQ ID NO: 21) 5′-CGTCAAAATCTCGGGAAACAGGTGTACCCTCAGCATTAAATTCGAG GTTGGCAGGTTGTATGGAGTAGTGTTTCACGTAAGTTACTCGTCTTACA GGCGGTGGCTCGATCTTAGCGATGTGTGTAAGGCTGCGCAAATTTATTC CGGGGATCCGTCGACC-3′
KanR-fabB_R:






(SEQ ID NO: 22)



5′-GTACACTTGTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGCC



GCATCAGGCGCCTC-3′.














The forward primer contained 140 base pairs with homology to the upstream region of the fabB gene in E. coli W3110K (SEQ ID NO: 1) and the resulting cassette contained a constitutive promoter, kanamycin resistance gene, and a transcriptional terminator. The PCR reaction was carried out using the enzyme Herculase II fusion DNA polymerase (Agilent Technologies, Inc., Santa Clara, Calif.) with an initial denaturation step at 95° C. for 2 min., followed by 30 cycles of the steps: 95° C. for 20 sec; 63° C. for 20 sec and 72° C. for 2 min. The denaturation step was followed by a final elongation step at 72° C. for 3 min. After the PCR reaction, the PCR product was purified through a PCR purification column and eluted with water.
Second, a chloramphenical marker was PCR amplified from pKD32 (Proc Natl Acad Sci USA. 2000 Jun. 6: 97(12):6640-5.) as described above with the following primers:
ChlorR-fabB_F:
(SEQ ID NO: 23) 5′-TGGTTCCCTTTTTCACATCATTGACAATCGCCGATTCCGGGGATC CGTCGACC-3′
Ultra_homolFabB3′CamRev: 5′-






(SEQ ID NO: 24)



5′-ATTACTCCAGAATGCAGCGCAAGGCGAGGAGTATCCCCGTCTCATCT



CTCTGGTTTCAGGGTTACGGTGCGTTGGCAGGATTTAACGCGTACGTCTT



TTCAGAAGGAAATCGACAAAGCGGGAAGTTTGCCTGGAACTGGTGTAGGC



TGGAGCTGCTTCG-3′.


















The reverse chloramphenicol primer contained 140 base pairs with homology to the downstream region of the fabB gene in E. coli W3110K (SEQ ID NO:1) and the resulting cassette contained a constitutive promoter for expressing the chloramphenicol resistance gene.
Third, the fabB library of variants (from Example 6) was PCR amplified as described above with the following primers:







FabB_F1



(SEQ ID NO: 25)





5′-GGCTGATCGGACTTGTTCGGCGTACAAG


and





FabB_R1



(SEQ ID NO: 26)





5′-CGGCGATTGTCAATGATGTGAAAAAGG.



























Finally, the two antibiotic resistance markers and the fabB library of variants were assembled using the technique of gene splicing by overlap extension PCR (SOE) (Warrens et al., Gene 1997, 186(1):29-35). One microliter of each of the two antibiotic resistance cassettes and the fabB library were mixed and the assembled product was amplified with the following primers:







Rescue_Fwd2:



(SEQ ID NO: 27)





5′-CGTCAAAATCTCGGGAAACAGGTG-3′


and





Rescue_Rev1:



(SEQ ID NO: 28)





5′-ATTACTCCAGAATGCAGCGCAAGG-3′.



























The SOE amplification reaction was carried out using the enzyme PfuUltra II Fusion DNA polymerase (Agilent Technologies. Inc., Santa Clara, Calif.) with an initial denaturation step at 95° C. for 2 min, followed by 30 cycles of the steps: 95° C. for 20 sec; 52° C. for 20 sec and 72° C. for 4 min. The denaturation step was followed by a final elongation step at 72° C. for 4 min. After the PCR reaction, the PCR product was purified through a PCR purification column and eluted with water. The product contained DNA bands of several sizes including the desired band of ˜3.9 kb.
Next, the genomic fabB in E. coli W3110K was replaced with the kanR-term-fabB library-chlorR cassette using the known technique of lambda RED-mediated homologous recombination as described below.
Strain W3110K was transformed with plasmid pSIM-CDX. Cells grown to log-phase at 32° C. were induced at 42° C. for 15 minutes and electrocompetent cells were prepared as described by Datta, Costantino, and Court (2006) Gene 379: 109-115. Competent cells were transformed with 500 ng of the fabB library cassette from above. Cells were recovered at 37° C. for four hours, plated on LB agar plates containing 20 micrograms/ml of kanamycin and 15 micrograms/ml of chloramphenicol, and incubated overnight at 37° C. Several colonies were PCR verified to have the genomic fabB gene replaced with the double antibiotic resistance cassette encoding unique fabB variants.
Example 8
Transforming pCDX11-V1 into the E. coli fabB Variant Library
The plasmid pCDX11-V1 was transformed into the E. coli fabB variant library as described below.
First, the colonies from the kanamycin/chloramphenicol selection plates described in Example 7 were pooled, grown in LB media to an OD 600 of ˜0.6, and concentrated 100-fold by centrifugation. The cells were washed three times with ice-cold sterile water, and then washed once with ice-cold 10% glycerol. The plasmid pCDX11-V1 was transformed into the pooled cells using standard molecular biology methods (Dower et al., 1988 NAR 16:6127-6145). The cells were recovered at 37° C. for an hour and plated on LB agar plates containing 100 micrograms/ml of spectinomycin. The plates were incubated overnight at 37° C.
Example 9
Production and Isolation of Fatty Alcohols
Recombinant E. coli host strains comprising a plasmid including heterologous genes as specified above were grown in M9 medium (Sambrook et al., (2001) Molecular Cloning: A Laboratory Manual. 3 rd ed., Cold Spring Harbor Laboratory Press, New York) supplemented with 1% glucose, 2 g/l yeast extract and the specified antibiotic selection, for approximately 16-18 hours (overnight) at 30° C., 200 rpm. A 5% inoculum was used to initiate fresh M9 media, 5% glucose and 2 g/l yeast extract containing the spectinomycin at 100 micrograms ml −1 when pCDX11 vector is used. The culture was incubated in a shaker for 2.5 hours at 30° C. and at 250 rpm to an OD 600 of about 0.6 to about 0.8. The expression of the heterologous FAR was then induced with isopropyl-β-D-thiogalactoside (IPTG) (1 mM final concentration). Incubation was continued for about 48 hours under the same conditions. Fatty acid species including fatty alcohols were extracted using 1 mL of methyl isobutyl ketone (MIBK) into 500 μl of cell culture, sealed tightly and shaken for ≧2.5 h. The extract was centrifuged and analyzed directly by GC-FID. A 1 μL sample was analyzed by GC-FID with the split ratio 1:10 using the following conditions: GC-6890N from Agilent Technologies equipped with FID detector and HP-5 column (length 30 m, I.D. 0.32 mm, film 0.25 μm). GC method: start at 100° C., increase the temperature with a rate of 25° C./min to 246° C. and hold for 1.96 min. Total run time was 7.8 min. Under the above GC conditions, the approximate retention times (min) of produced fatty alcohols and acids were as follows: 1.81, C10:0-OH; 2.41, C12:1-OH; 2.45, C12:0-OH; 3.17, C14:1-OH; 3.22, C14:0-OH; 5.40, C14:0-OOH; 3.95, C16:1-OH; 4.02, C16:0-OH; 4.20, C16:0-OOMe (internal standard); 6.16, C16:1-OOH; 6.29, C16:0-OOH; 4.89, C18:1-OH; 5.00, C18:0-OH; and 7.3, C18:0- and C18:1-OOH. Identification of individual fatty alcohols was determined by comparison to commercial standards (Sigma Chemical Company, 6050 Spruce St. Louis, Mo. 63103).









TABLE 1



Variant
Total C12
% 12:0-OH
Total C14
Total Titer

No. 1.
FIOP 2
FIOP 3
FIOP 2
FIOP











P303C
1.26
1.39
0.89
0.95

P303V
1.19
1.20
0.94
1.00

S42E
1.22
1.15
1.06
1.05

A206G
1.43
1.17
1.18
1.22

R45G
1.38
1.05
1.39
1.33

L335M
1.26
1.11
1.14
1.13

K217V
1.11
1.08
1.02
1.02

S42L
1.27
1.17
1.09
1.07

E216W
1.04
1.07
0.92
0.99

Q367V
1.03
1.04
0.98
1.00

K40G
1.04
1.04
1.00
1.01

K40S/P303R
1.13
1.15
0.95
0.99

K40A
0.98
0.96
1.02
1.01

K40R
1.13
1.08
1.04
1.04

I231F
1.52
1.27
1.14
1.22

I231C
1.15
1.23
—
0.93



1. The amino acid residue substitution modification is relative to the wild-type amino acid residue in the corresponding position of the FabB of SEQ ID NO: 2. The FIOP values are averaged from multiple selections of 1 to 10 from primary screens.

2. Total C12 FIOP or total C14 FIOP is the total amount of C12 or C14 fatty alcohols, respectively, produced (saturated and unsaturated) and measured by fold improvement over the parent control (FIOP) wherein parent = 1.0.

3. % C12:0-OH is the % of saturated C12 fatty alcohols measured by fold improvement over the total % C12 of the parent control.






































Each publication, patent, patent application, or other document cited in this application is hereby incorporated by reference in its entirety for all purposes to the same extent as if each were individually indicated to be incorporated by reference for all purposes in the specification directly adjacent the citation.
While various specific embodiments have been illustrated and described, it will be appreciated that various changes can be made without departing from the spirit and scope of the invention(s).