(en)The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.

1.ApplicationNumber: US-4859908-A
1.PublishNumber: US-7968699-B2
2.Date Publish: 20110628
3.Inventor: HAEFNER STEFAN
SCHRODER HARTWIG
ZELDER OSKAR
KLOPPROGGE CORINNA
HEROLD ANDREA

4.Inventor Harmonized: HAEFNER STEFAN(DE)
SCHRODER HARTWIG(DE)
ZELDER OSKAR(DE)
KLOPPROGGE CORINNA(DE)
HEROLD ANDREA(DE)

5.Country: US
6.Claims:
(en)The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.

7.Description:
(en)RELATED APPLICATIONS
This application is a divisional of U.S. application Ser. No. 11/793,909, filed Jun. 21, 2007 which is a 35 U.S.C. 371 National stage filing of International Application No. PCT/EP2005/013809, filed Dec. 21, 2005, which claims priority to German Application No. 10 2004 061 846.1, filed Dec. 22, 2004. The entire contents of each of these applications are hereby incorporated by reference herein.


SEQUENCE LISTING SUBMISSION
The Sequence Listing associated with this application is filed in electronic format via EFS-Web and hereby incorporated by reference into the specification in its entirety. The name of the text file containing the Sequence Listing is Sequence_List — 12810 — 00672_US. The size of the text file is 174 KB, and the text file was created on Jun. 16, 2008.
DESCRIPTION
The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.
BACKGROUND OF THE INVENTION
Various biosynthetic products are produced in cells via natural metabolic processes and are used in many branches of industry, including the foodstuffs, feedstuffs, cosmetics, feed, food and pharmaceutical industries. These substances, which are summarily referred to as fine chemicals/proteins, comprise, inter alia, organic acids, both proteinogenic and nonproteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and also proteins and enzymes. They are most conveniently produced on a large scale by growing bacteria strains or other microorganisms which have been developed in order to produce and secrete large quantities of the substance desired in each case. Organisms particularly suitable for this purpose are coryneform bacteria, Gram-positive nonpathogenic bacteria.
It is known that amino acids can be prepared by fermentation of strains of coryneform bacteria, in particular Corynebacterium glutamicum . Due to the great importance, continuous work is carried out on improving the production processes. Process improvements may relate to fermentation technique measures such as, for example, stirring and oxygen supply, or to the composition of the nutrient media, such as, for example, the sugar concentration during fermentation, or to the work-up to obtain the product, for example by ion exchange chromatography or else spray drying, or to the intrinsic performance properties of the microorganism itself.
Methods of recombinant DNA technology have likewise been employed for some years to improve Corynebacterium strains producing fine chemicals/proteins, by amplifying individual genes and studying the effect on the production of fine chemicals/proteins.
Other ways of developing a process for producing fine chemicals or proteins or of increasing or improving the productivity of an already existing process for preparing fine chemicals or proteins comprise increasing or altering expression of one or more genes and/or influencing translation of an mRNA by way of suitable polynucleotide sequences. In this context, influencing may comprise increasing, reducing or else other parameters of the expression of genes, such as time-related expression patterns.
Various components of bacterial regulatory sequences are known to the skilled worker. A distinction is made between the binding sites of regulators, also known as operators, the binding sites of RNA polymerase holoenzymes, also known as −35 and −10 regions, and the binding site of ribosomal 16S-RNA, also known as ribosomal binding site (RBS) or else Shine-Dalgarno sequence.
The composition of the polynucleotide sequence of the Shine-Dalgarno sequence and the sequence of the bases, but also the distance of a polynucleotide sequence present in the Shine-Dalgarno sequence to the start codon are described in the literature ( E. coli and S. typhimurium , Neidhardt F. C. 1995 ASM Press) as having a substantial influence on the rate of translation initiation.
Nucleic acid sequences having promoter activity can influence the formation of mRNA in different ways. Promoters whose activities are independent of the physiological growth phase of the organism are referred to as constitutive. Other promoters in turn respond to external chemical as well as physical stimuli, such as oxygen, metabolites, heat, pH, etc. Others in turn display a strong dependence of their activity in different growth phases. Examples of promoters which exhibit a particularly pronounced activity during the exponential growth phase of microorganisms, or else exactly in the stationary phase of microbial growth, are described in the literature. Both characteristics of promoters may have a beneficial influence on the productivity for production of fine chemicals and proteins, depending on the metabolic pathway.
Those nucleotide sequences which may be utilized for increasing or attenuating gene expression have already been isolated in Corynebacterium species. These regulated promoters may increase or reduce the rate at which a gene is transcribed, as a function of the internal and/or external conditions of the cell. In some cases, the presence of a particular factor, known as inducer, may stimulate the rate of transcription from the promoter. Inducers may influence transcription from the promoter directly or else indirectly. Another class of factors, known as suppressors, is capable of reducing or else inhibiting transcription from the promoter. Like inducers, suppressors may also act directly or indirectly. However, thermally regulated promoters are also known. Thus, a rise of the growth temperature above the normal growth temperature of the cell may increase or else attenuate the level of transcription of such promoters.
A small number of promoters from C. glutamicum have been described to date. The promoter of the C. glutamicum malate synthase gene was described in DE-A-44 40 118. This promoter was placed upstream of a structural gene coding for a protein. After transformation of such a construct into a coryneform bacterium, the expression of the structural gene downstream of the promoter is regulated. The expression of the structural gene is induced as soon as a corresponding inducer is added to the medium.
Reinscheid et al., Microbiology 145:503 (1999), have described a transcriptional fusion between the pta-ack promoter from C. glutamicum and a reporter gene (chloramphenicol acetyltransferase). C. glutamicum cells containing such a transcriptional fusion exhibited increased expression of the reporter gene when grown on acetate-containing medium. By comparison, transformed cells growing on glucose showed no increased expression of said reporter gene.
Patek et al., Microbiology 142:1297 (1996), described some C. glutamicum DNA sequences which are able to enhance expression of a reporter gene in C. glutamicum cells. These sequences were compared to one another in order to define consensus sequences for C. glutamicum promoters
Further C. glutamicum DNA sequences which may be utilized for regulating gene expression have been described in WO 02/40679. These isolated polynucleotides are expression units from Corynebacterium glutamicum , which may be utilized either for increasing or else for reducing gene expression. This printed publication furthermore describes recombinant plasmids on which said expression units from Corynebacterium glutamicum are associated with heterologous genes. The method described herein, of fusing a Corynebacterium glutamicum promoter to a heterologous gene, may be employed, inter alia, for regulating the genes of amino acid biosynthesis.
The older, not previously published patent applications DE-A-103 59 594, DE-A-103 59 595, DE-A-103 59 660 and DE-A-10 2004 035 065 disclosed special single promoters from C. glutamicum.
BRIEF DESCRIPTION OF THE INVENTION
The invention was based on the object of making available further regulatory nucleic acid sequences, in particular promoter constructs and/or expression units having advantageous properties, for example increased or altered transcriptional activity and/or translational activity, in comparison with the starting promoter.
The object was achieved according to the invention by providing a multiple promoter-comprising expression unit, comprising, in the 5′-3′ direction, a sequence module of the following formula I:

5′-P 1 -(-A x -P x -) n -A y -P y -3′ (I)

where

n is an integer from 0 to 10,

A x and A y are identical or different and are a chemical bond or a linker nucleic acid sequence;

P 1 , P x and P y code for identical or different promoter sequences which comprise at least one RNA polymerase-binding region such as, for example, the core region; and at least P y comprises a ribosome binding-mediating, 3′-terminal sequence section. P 1 and individual or all P x may also have, independently of one another, a sequence section of this kind, which mediates ribosome binding.

According to one embodiment, P 1 , P x and P y are derived from identical or different eukaryotic or, in particular, prokaryotic organisms. Artificial promoters are also usable.
According to a preferred embodiment, P 1 , P x and P y are in each case derived from a contiguous sequence of from 35 to 500 nucleotide residues, preferably 35 to 300 nucleotide residues, more preferably 35 to 210 nucleotide residues, and very particularly preferably 35 to 100 nucleotide residues, which sequence is located 5′ upstream of the coding sequence for a protein, for example a protein involved in a biosynthetic pathway of the organism, in the genome of said organism.
According to another embodiment, P 1 , P x and P y of the expression unit are derived from a coryneform bacterium.
According to a preferred embodiment, P 1 , P x and P y of the expression unit are, independently of one another, selected from among promoter sequences for the coding sequence of a protein with high abundance in a eukaryotic or prokaryotic organism, in particular in a coryneform bacterium, such as, for example, in one of the genus Corynebacterium or Brevibacterium , for example a species or a strain according to the list in table 3.
In addition, individual or all of these promoter sequences may be selected from among promoter sequences for the coding sequence of a protein listed in table 1 and involved in the amino acid biosynthesis of an organism. At least one of the promoters of the expression unit according to the invention should, in this connection, have high abundance as defined herein, however.
According to another preferred embodiment, P 1 , P x and P y of the expression unit are, independently of one another, selected from among the nucleotide sequences depicted in FIG. 1 or, as will be explained later in more detail, are derived from SEQ ID NO:1 (pGRO), SEQ ID NO:2 (pEFTs), SEQ ID NO:3 (pEFTu) and SEQ ID NO:4 (pSOD).
According to another embodiment, P 1 , P x and P y of the expression unit, independently of one another, are selected from among strong, constitutive or regulatable promoters.
In a further aspect, the present invention relates to an expression cassette, comprising, in the 5′-3′ direction, a sequence module of the following formula II:

5′-P 1 -(-A x -P x -) n -A y -P y -G-3′ (II)

where

n, A x , A y , P 1 , P x and P y are as defined above, and

G is at least one coding nucleic acid sequence which is functionally or operatively linked to the 5′ upstream regulatory sequence.

According to a preferred embodiment of the expression cassette, G is selected from among
a) nucleic acids encoding a protein of the biosynthetic pathway of proteinogenic and nonproteinogenic amino acids, b) nucleic acids encoding a protein of the biosynthetic pathway of nucleotides and nucleosides, c) nucleic acids encoding a protein of the biosynthetic pathway of organic acids, d) nucleic acids encoding a protein of the biosynthetic pathway of lipids and fatty acids, e) nucleic acids encoding a protein of the biosynthetic pathway of diols, f) nucleic acids encoding a protein of the biosynthetic pathway of carbohydrates, g) nucleic acids encoding a protein of the biosynthetic pathway of aromatic compounds, h) nucleic acids encoding a protein of the biosynthetic pathway of vitamins, i) nucleic acids encoding a protein of the biosynthetic pathway of cofactors, j) nucleic acids encoding a protein of the biosynthetic pathway of enzymes, and k) nucleic acids encoding a protein of the central metabolism.

According to a particularly preferred embodiment of the expression cassette, G is selected from among nucleic acids coding for proteins of the biosynthetic pathway of amino acids, selected from among aspartate kinase, aspartate semialdehyde dehydrogenase, diaminopimelate dehydrogenase, diaminopimelate decarboxylase, dihydrodipicolinate synthetase, dihydrodipicolinate reductase, glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, pyruvate carboxylase, triosephosphate isomerase, transcriptional regulator LuxR, transcriptional regulator LysR1, transcriptional regulator LysR2, malate-quinone oxidoreductase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, transketolase, transaldolase, homoserine O-acetyltransferase, cystathionine gamma-synthase, cystathionine beta-lyase, serine hydroxymethyltransferase, O-acetylhomoserine sulfhydrylase, methylenetetrahydrofolate reductase, phosphoserine aminotransferase, phosphoserine phosphatase, serine acetyltransferase, homoserine dehydrogenase, homoserine kinase, threonine synthase, threonine exporter carrier, threonine dehydratase, pyruvate oxidase, lysine exporter, biotin ligase, cysteine synthase 1, cysteine synthase II, coenzyme B12-dependent methionine synthase, coenzyme B12-independent methionine synthase activity, sulfate adenylyltransferase subunits 1 and 2, phosphoadenosine phosphosulfate reductase, ferredoxin sulfite reductase, ferredoxin NADP reductase, 3-phosphoglycerate dehydrogenase, RXA00655 regulator, RXN2910 regulator, arginyl-tRNA synthetase, phosphoenolpyruvate carboxylase, threonine efflux protein, serine hydroxymethyltransferase, fructose 1,6-bisphosphatase, protein of sulfate reduction RXA077, protein of sulfate reduction RXA248, protein of sulfate reduction RXA247, OpcA protein, 1-phosphofructokinase and 6-phosphofructokinase, tetrahydropicolinate succinylase, succinyl aminoketopimelate aminotransferase, succinyl diaminopimelate desuccinylase, diaminopimelate epimerase, 6-phosphogluconate dehydrogenase, glucose phosphate isomerase, phosphoglycerate mutase, enolase, pyruvate kinase, aspartate transaminase, malate enzyme.
In a further aspect, the present invention relates to a vector which comprises at least one of the abovementioned expression cassettes.
According to yet another aspect, the present invention relates to a genetically modified microorganism which has been transformed with at least one of the above-mentioned vectors or which comprises at least one of the abovementioned expression cassettes, preferably in an integrated form.
According to one embodiment, the generally modified organism is derived from coryneform bacteria.
According to a preferred embodiment, the genetically modified organism is derived from bacteria of the genus Corynebacterium or Brevibacterium.
According to a further aspect, the present invention relates to a process for preparing a biosynthetic product, which comprises culturing any of the abovementioned, genetically modified microorganisms and isolating the desired product from the culture.
According to one embodiment of the process, the biosynthetic product is selected from among organic acids, proteins, nucleotides and nucleosides, both proteinogenic and nonproteinogenic amino acids, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, enzymes and proteins. Very particularly preferred biosynthetic products are lysine, methionine, threonine and trehalose.
According to a further aspect, the present invention relates to the use of an expression unit of the invention for regulating a product biosynthesis.



DESCRIPTION OF THE FIGURES
FIG. 1 depicts specific sequences for four promoters, which were used for preparing the multiple promoters described in the exemplary embodiments. The promoter sequences for pEFTu ( FIG. 1A ); for the promoter pGRO ( FIG. 1B ), for the promoter pEFTs ( FIG. 1C ) and for the promoter pSOD ( FIG. 1D ) are shown. Two sequences are indicated for each promoter, the longer of which (top sequence) in each case indicating the complete promoter sequence including the ribosomal binding site (RBS), printed in bold type and in italic. In the case of a 3′-terminal arrangement of the particular promoter in the multiple promoter construct of the invention, preference is given to using in each case the longer nucleotide sequence (including RBS). Promoter sequences 5′ upstream of the 3′-terminal promoter unit do not comprise any RBS and were used in the truncated nucleic acid sequence indicated in each case in second position (without RBS). The shorter promoter sequences may also lack, if appropriate, 3′-terminal partial sequences indicated in capital letters and bold type. Potential “−10 regions” are underlined.



DETAILED DESCRIPTION OF THE INVENTION
I. General Definitions
A “biosynthetic product” means for the purposes of the invention those which are produced via natural metabolic processes (biosynthetic pathways) in cells. They are used in many different ways, in particular in the foodstuffs, feedstuffs, cosmetics, feed, food and pharmaceutical industries. These substances which are summarily referred to as fine chemicals/proteins comprise, inter alia, organic acids, both proteinogenic and nonproteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and also proteins and enzymes.
A “promoter”, a “nucleic acid with promoter activity” or a “promoter sequence” means according to the invention a nucleic acid which is functionally linked to a nucleic acid to be transcribed and which regulates transcription of said nucleic acid.
Unless “single promoters” are expressly referred to, the term “promoter” comprises according to the invention and depending on the context also the sequential arrangement of more than one nucleic acid sequence (i.e. “multiple promoters”) each of which, when functionally linked to a nucleic acid to be transcribed, would be capable of regulating transcription of the latter.
The term “single promoter” accordingly refers to a single nucleic acid sequence which is capable of regulating transcription of a nucleic acid to be transcribed and which, when functionally linked to a nucleic acid to be transcribed, can transcribe the latter.
In the case of “multiple promoters”, at least two identical or different nucleic acid sequences each of which would, when functionally linked to a nucleic acid to be transcribed, be capable of regulating transcription of the latter (single promoters) are sequentially linked in such a way that a transcriptional start from optionally one of said nucleic acid sequences could produce a transcript containing said nucleic acid to be transcribed. In this connection, it is possible for further sequences without promoter function, for example a linker which has, for example, one or more restriction cleavage sites, to be inserted between in each case two single promoters. Optionally, in each case two single promoters may also be linked directly to one another. Said multiple promoters preferably contain only one ribosomal binding site (RBS), in particular in the region of the 3′ terminus of the promoter.
Promoter sequences for the coding sequence of a protein with “high abundance” means in particular “strong promoters”. If it is intended to employ multiple promoters in order to enhance genes, preference is given to combining in a suitable manner such strong promoters. Strong promoters regulate transcription of genes so as for the latter to be more frequently read by RNA polymerase than the majority of the genes of the organism. In most cases, the presence of a strong promoter results in a large amount of transcript also being present in the cell. When the percentage of said transcript is higher in comparison with the other cellular transcripts, this is referred as an “abundant transcript”. In bacteria, the amount of transcript and the amount of the corresponding protein encoded thereby usually correlate, i.e. “abundant transcripts” also result in “abundant proteins” most of the time. A method which allows “strong promoters” to be identified via the abundance of proteins is described below by way of example. Details of the methodical procedure and further references can be found, for example, in Proteomics in Practice—A Laboratory Manual of Proteome Analysis (Authors: R. Westermeier, T. Naven; Publisher: Wiley-VCH Verlag GmbH, 2002). The bacterial cells are disrupted and the proteins of the cell extract are fractionated by means of 2D gel electrophoresis. The proteins are then stained by common methods such as, for example, Coomassie, silver or fluorescent dye staining. Overstaining of the proteins is to be avoided here in order to enable the individual protein spots to be quantified later. Subsequently, the gel is scanned and the image obtained is analyzed with the aid of suitable software (e.g. Melanie, Amersham Biosciences). For this purpose, all detectable spots are identified first and the spot volume is determined. The sum of all spot volumes corresponds, in a first approximation, to the total protein. The spots which have particularly large spot volumes may then be selected with the aid of the image analysis software. For example, spots with volumes occupying more than 0.1% of the total spot volume may be referred to as abundant. Subsequently, spots containing particularly abundant proteins are cut out of the gel. Normally, the protein present in the gel slice is then proteolytically digested (e.g. with trypsin) and the molar mass of the resultant peptides is determined, for example with the aid of MALDI-ToF MS (Matrix assisted laser desorption ionization-Time of Flight Mass Spectrometry). Based on the molar mass of some of the tryptic peptides of the protein, the corresponding protein may then be identified in database searches. This is possible in particular if the genome of the organism to be studied has been sequenced, as is the case for C. glutamicum, E. coli and many other bacteria. The ORF (open reading frame) encoding said protein may then be determined based on the identity of said protein. In bacteria, the promoters regulating said gene are usually located immediately upstream of the start codon. Therefore, the “strong” promoters are frequently also present in a region of approx. 200 nucleotides upstream of the start codon of “abundant” proteins.
“Artificial” promoters for the purposes of the invention comprise in particular those sequences which have no transcriptional activity in situ and which are transcriptionally active in another sequence context, such as, in particular, in the context of an expression unit or expression cassette of the invention.
“Promoter activity” means according to the invention the amount of RNA formed by the promoter in a particular time, i.e. the rate of transcription.
“Specific” promoter activity means according to the invention the amount of RNA formed by the promoter in a particular time, for each promoter.
In the case of a “caused” promoter activity or rate of transcription with respect to a gene, in comparison with the wild type, thus, in comparison with the wild type, the formation of an RNA which has not been present in this form in the wild type is caused.
In the case of an “altered” promoter activity or rate of transcription with respect to a gene, in comparison with the wild type, thus, in comparison with the wild type, the amount of RNA formed in a particular time is altered.
“Altered” in this connection means preferably increased or reduced.
A “functional” or “operative” linkage means in this connection, for example, the sequential arrangement of any of the nucleic acids of the invention which have promoter activity and a nucleic acid sequence to be transcribed and, if appropriate, further regulatory elements such as, for example, nucleic acid sequences which ensure transcription of nucleic acids, as well as a terminator, for example, so as for each of said regulatory elements to be able to fulfill its function in transcription of said nucleic acid sequence. This does not necessarily require a direct linkage in the chemical sense. Genetic control sequences such as, for example, enhancer sequences can exert their function on the target sequence also from more distant positions or even from other DNA molecules. Preference is given to arrangements in which the nucleic acid sequence to be transcribed is positioned behind (i.e. at the 3′ end of) the promoter sequence of the invention, so that the two sequences are covalently connected to one another. The distance between the promoter sequence and the nucleic acid sequence to be expressed transgenically is here preferably less than 200 base pairs, particularly preferably less than 100 base pairs, very particularly preferably less than 50 base pairs.
The sequence of a “ribosomal binding site” or “ribosome binding site” (RBS) (or referred to as Shine-Dalgarno sequence) in accordance with the present invention means A/G-rich polynucleotide sequences which are located up to 30 bases upstream of the translation initiation codon.
“Transcription” means according to the invention the process which, starting from a DNA template, produces a complementary RNA molecule. This process involves proteins such as RNA polymerase, “sigma factors” and transcriptional regulatory proteins. The synthesized RNA then serves as template in the translation process which then results in the biosynthetically active protein.
A “core region” of a promoter means according to the invention a contiguous nucleic acid sequence of from about 20 to 80, or 30 to 60, nucleotides, which comprises at least one potential “−10 region”. Examples of potential −10 regions are described herein for individual preferred promoter sequences; compare FIG. 1 in particular. “−10 regions” are also referred to as TATA boxes or Pribnow-Schaller sequences. Each promoter used should comprise at least one of these potential −10 regions, for example 1, 2, 3, 4 or 5. The core region is located 5′ upstream of the RBS and may be at a distance from the latter of from 1 to 200 or 10 to 150 or 20 to 100 nucleotide residues.
An “expression unit” means according to the invention a nucleic acid having expression activity, which comprises a multiple promoter as defined herein and which, after functional linkage to a nucleic acid or a gene to be expressed, regulates expression, i.e. transcription and translation, of said nucleic acid or said gene. In this connection, therefore, said nucleic acid sequence is also referred to as a “regulatory nucleic acid sequence”. In addition to the multiple promoter construct, further regulatory elements such as, for example, enhancers, may be present.
An “expression cassette” means according to the invention an expression unit which is functionally linked to the nucleic acid to be expressed or the gene to be expressed. In contrast to an expression unit, an expression cassette thus comprises not only nucleic acid sequences regulating transcription and translation but also the nucleic acid sequences which are to be expressed as protein as a result of transcription and translation.
“Expression activity” means according to the invention the amount of protein formed by the expression cassette or its expression unit in a particular time, i.e. the rate of expression.
“Specific expression activity” means according to the invention the amount of protein formed by the expression cassette or its expression unit in a particular time, for each expression unit.
In the case of a “caused expression activity” or rate of expression with respect to a gene, in comparison with the wild type, thus, in comparison with the wild type, the formation of an protein which has not been present in this form in the wild type is caused.
In the case of an “altered” expression activity or rate of expression with respect to a gene, in comparison with the wild type, thus, in comparison with the wild type, the amount of protein formed in a particular time is altered. “Altered” in this connection means preferably increased or reduced. This may take place, for example, by increasing or reducing the specific activity of the endogenous expression unit, for example by way of mutation, or by way of stimulation or inhibition. The altered expression activity or rate of expression may furthermore be achieved, for example, by regulating expression of genes in the microorganism by expression units of the invention, the genes being heterologous with respect to the expression units.
The “rate of formation” at which a biosynthetically active protein is produced is a product of the rates of transcription and translation. Both rates may be influenced according to the invention and thus influence the rate of formation of products/fine chemicals in a microorganism.
The term “wild type” means according to the invention the appropriate starting microorganism and need not necessarily correspond to a naturally occurring organism.
Depending on the context, the term “microorganism” may mean the starting microorganism (wild type) or a genetically modified microorganism of the invention or both.
Preferably, and in particular in cases where it is not possible to assign the microorganism or the wild type unambiguously, “wild type” for altering or causing the promoter activity or rate of transcription, for altering or causing the expression activity or rate of expression and for increasing the biosynthetic product content in each case means a “reference organism”. In a preferred embodiment, this “reference organism” is Corynebacterium glutamicum ATCC 13032 or a microorganism derived from ATCC 13032 by specific or unspecific mutation.
Use is made in particular of “starting microorganisms” which are already capable of producing the desired product (fine chemical/protein).
A “derived” sequence, for example a derived promoter sequence, means according to the invention, if no other information is given, a sequence whose identity with the starting sequence is at least 80% or at least 90%, in particular 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% and 99%.
“Identity” between two nucleic acids means the identity of the nucleotides over the entire length of the nucleic acid in each case, in particular the identity calculated by comparison with the aid of the Vector NTI Suite 7.1 software from Informax (USA), applying the Clustal method (Higgins D G, Sharp P M. Fast and sensitive multiple sequence alignments on a microcomputer. Comput Appl. Biosci. 1989 April; 5(2):151-1), setting the following parameters:
Multiple Alignment Parameter:
Gap opening penalty 10
Gap extension penalty 10
Gap separation penalty range 8
Gap separation penalty off
% identity for alignment delay 40
Residue specific gaps off
Hydrophilic residue gap off
Transition weighing 0
Pairwise Alignment Parameter:
FAST algorithm on
K-tuple size 1
Gap penalty 3
Window size 5
Number of best diagonals 5
“To hybridize” means the ability of a poly- or oligonucleotide to bind under stringent conditions to a virtually complementary sequence, while unspecific bonds between noncomplementary partners are not formed under these conditions. For this purpose, the sequences should preferably be 90-100% complementary. The property of complementary sequences being able to bind specifically to one another is utilized, for example, in the Northern or Southern Blotting Technique or for primer binding in PCR or RT-PCR.
According to the invention, “hybridization” takes place under stringent conditions. Hybridization conditions of this kind are described, for example, in Sambrook, J., Fritsch, E. F., Maniatis, T., in: Molecular Cloning (A Laboratory Manual), 2nd edition, Cold Spring Harbor Laboratory Press, 1989, pages 9.31-9.57 or in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. Stringent hybridization conditions mean in particular:
Incubation at 42° C. overnight in a solution consisting of 50% formamide, 5×SSC (750 mM NaCl, 75 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5×Denhardt's solution, 10% dextrane sulfate and 20 g/ml denatured, sheared salmon sperm DNA, followed by washing the filters with 0.1×SSC at 65° C.
A “functionally equivalent fragment” means for nucleic acid sequences having promoter activity fragments which essentially have the same or an altered, lower or higher specific promoter activity than the starting sequence.
“Essentially identical” means a specific promoter activity which has at least 50%, such as, for example, at least 60%, 70%, 80%, 90%, or at least 95%, of the specific promoter activity of the starting sequence.
II. Expression Units, Expression Cassettes and Components Thereof
a) Expression Units
The present invention relates inter alia to providing a multiple promoter-comprising expression unit, comprising, in the 5′-3′ direction, a sequence module of the following formula I:

5′-P 1 -(-A x -P x -) n -A y -P y -3′

where

n is an integer from 0 to 10, such as for example 1, 2, 3, 4, 5 or 6;

A x and A y are identical or different and are a chemical, in particular covalent bond, or a chemically, in particular covalently, integrated linker nucleic acid sequence;

P 1 , P x and P y code for identical or different promoter sequences which comprise at least one RNA polymerase-binding region such as, for example, a core region; and at least, for example only, P y comprises a ribosome binding-mediating, 3′-terminal sequence section.

The promoter sequences P 1 , P x and P y may be derived from genes from organisms, which code for proteins involved in a biosynthetic pathway of said organism. The promoter sequences here are located from 20 to 500 nucleotide residues, or from 20 to 300 nucleotide residues, for example from 20 to 210 nucleotide residues, and in particular from 20 to 100 or 35 to 100 nucleotide residues, 5′ upstream of the coding sequence of the particular protein in the genome of said organism. Examples of sequences from which promoter sequences P 1 , P x and P y may be derived are the genes listed in Table 1 below.
Nonlimiting examples of special promoter sequences are derived from the nucleic acid sequences according to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, and FIG. 1 , without being limited thereto, however.
In this connection, SEQ ID NO:1 corresponds to the sequence located upstream of the coding region of the GroES Chaperonin (pGRO), SEQ ID NO:2 corresponds to the sequence located upstream of the coding region of protein elongation factor TS (pEFTs), SEQ ID NO:3 corresponds to the sequence located upstream of the coding region of protein elongation factor TU (pEFTu), and SEQ ID NO:4 corresponds to the sequence located upstream of the coding region of superoxide dismutase (pSOD), in each case from Corynebacterium glutamicum . SEQ. ID. NO. 1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4 correspond to the promoter sequences of the wild type.
A “derived” sequence means according to the invention a sequence which is at least 80% or at least 90%, such as 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, and in particular 99%, identical to the starting sequence. This degree of identity applies in particular to the above-defined “core region” of the particular promoter. The degree of identity outside the particular core region may be lower here and may be in the range from 0 to 80%, such as, for example, 10 to 80%, 20 to 70%, 30 to 60% or 40 to 50%.
Usable core regions are located, for example
for pGRO in the range from position 50 to 80 of SEQ ID NO:1;
for pEFTs in the range from position 130 to 170 of SEQ ID NO:2;
for pEFTu in the range from position 30 to 110 of SEQ ID NO:3;
for pSOD in the range from position 50 to 100 of SEQ ID NO:4.
The expression units of the invention contain in particular
two or more nucleic acid sequences having promoter activity,




a) preferably derived from identical or different sequences selected from among SEQ. ID. NO. 1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4; or other promoter sequences for genes with comparable or higher abundance;
b) with, if appropriate, one or more of said sequences representing a sequence derived by substitution, insertion, inversion or deletion or addition of nucleotides, which sequence is, in particular in the core region, at least 80% identical at the nucleic acid level to a sequence preferably selected from among sequence SEQ ID NO: 1, 2, 3 or 4 or other promoter sequences for genes with comparable or higher abundance,
c) or with, if appropriate, one or more of these nucleic acid sequences present hybridizing under stringent conditions with a sequence which is complementary to one of the nucleic acid sequences according to SEQ ID NO: 1, 2, 3 or 4 and/or to other promoter sequences for genes with comparable or higher abundance,
d) or with, if appropriate, one or more of these nucleic acid sequences present being “functionally equivalent fragments” of the sequences under a), b) or c).




Other natural promoters which are usable as a component of a multiple promoter of the invention can readily be identified, for example, from various organisms whose genomic sequence is known, by comparing the identities of the nucleic acid sequences from databases with the above-described sequences SEQ ID NO: 1, 2, 3 or 4 or according to FIG. 1 or other promoter sequences for genes with comparable or higher abundance.
Further suitable natural promoters can readily be identified from various organisms whose genomic sequence is not known by applying hybridization techniques in a manner known per se, starting from the above-described nucleic acid sequences, in particular starting from the sequence SEQ ID NO: 1, 2, 3 or 4 and/or from promoter sequences for genes with comparable or higher abundance.
Further suitable natural promoters can also be isolated by methods known to the skilled worker, as are described, for example, in Cadenas et al (1991) Gene 98, 117-121; Eikmanns et al, (1991) Gene 102, 93-98.; Patek et al (1996) Microbiology 142, 1297-1309 or Santamaria et al (1987) Gene 56, 199-208. This usually involves using “promoter probe” vectors into which genomic fragments from the genome in question are inserted. The promoter activity of an individual fragment can then be determined in the experimental setup mentioned by measuring the activity of a downstream reporter gene, for example chloramphenicol acetyltransferase.
The invention therefore also comprises the combination of those promoters which are not listed herein by name. This also applies to the combination of already known single promoters, as are described, for example, in Patek et al (2003). J of Biotechnology 104, 311-323; Vasicova et al. (1999) J. Bacteriol 181, 6188-6191).
The invention therefore also relates to nucleic acids having promoter activity for incorporation into a multiple promoter-comprising expression unit of the invention, said nucleic acids having promoter activity comprising a nucleic acid sequence which hybridizes with the nucleic acid sequence SEQ ID NO: 1, 2, 3 or 4 or with promoter sequences for genes with comparable or higher abundance under stringent conditions. This nucleic acid sequence comprises at least 10, preferably more than 12, 15, 30, 50, or more than 150, nucleotides.
Artificial promoter sequences for incorporation into a multiple promoter-comprising expression unit of the invention can readily be prepared starting from the sequence SEQ ID NO: 1, 2, 3 or 4 or according to FIG. 1 and/or promoter sequences for genes with comparable or higher abundance by way of artificial variation and mutation, for example by addition, substitution, insertion, inversion or deletion of one or more, individual or contiguous nucleotide residues. (Patek et al (2003). J of Biotechnology 104, 311-323; Vasicova et al. (1999) J. bacteriol 181, 6188-6191 and references mentioned therein).
Suitable examples of ribosome binding-mediating 3′-terminal sequence sections of a promoter sequence P y are RBS of pGRO: GGAGGGA; the RBS of pEFTs: AGGAGGA; the RBS of pEFTu: AGGAGGA; and the RBS of pSOD: GGAGGGA (cf. also FIG. 1 , attached). The theoretically optimal RBS, i.e. the sequence which is 100% complementary to the anti-Shine-Dalgarno sequence on the C. glutamicum 16S rRNA, is: 5′ GAAAGGAGG 3′. Other suitable RBS sequence sections can readily be identified, for example, by artificial variation and mutation, for example by substitution, insertion, inversion, addition or deletion of nucleotides, or via homology comparisons with promoter sequences in databases.
Nonlimiting examples of multiple promoter-comprising expression units of the invention are selected from among:
e) sequences coding for
PeftuPsod, from position 18 to 390 in SEQ ID NO: 45; PsodPeftu, from position 18 to 395 in SEQ ID NO: 52; PgroPsod, from position 18 to 369 in SEQ ID NO: 55; PgroPsodPefts, from position 11 to 538 in SEQ ID NO: 59; PeftuPsodPefts; from position 11 to 559 in SEQ ID NO: 60; or
f) sequences which are derived from sequences according to e) by substitution, insertion, inversion, addition or deletion of nucleotides and which, in comparison with said starting sequence, are at least 80% or at least 90% identical at the nucleic acid level; or g) nucleic acid sequences which hybridize with a sequence complementary to e) under stringent conditions; or

h) “functionally equivalent fragments” of the abovementioned sequences e), f) and g).


A “functionally equivalent fragment”, in particular according to the embodiments d) and h), means, for expression units, fragments which have essentially the same or a higher specific expression activity as/than the starting sequence.
“Essentially identical” means a specific expression activity which has at least 50%, preferably 60%, more preferably 70%, more preferably 80%, more preferably 90%, particularly preferably 95%, of the specific expression activity of the starting sequence.
“Fragments” means partial sequences of the sequences described by embodiment a) to h). Said fragments preferably have more than 10, but more preferably more than 12, 15, 30, 50, or particularly preferably more than 150, contiguous nucleotides of the starting sequence.
The expression units of the invention may preferably comprise one or more of the following genetic elements: a −10 region; a transcription start, an enhancer region; and an operator region.
These genetic elements are preferably specific for the species corynebacteria , especially for Corynebacterium glutamicum.
Bacterial promoters normally consist of three RNA polymerase recognition sites, namely the −10 region, the −35 region and the UP element. These promoter elements are highly conserved in E. coli , but not in C. glutamicum . This applies especially to the -35 region. The latter can therefore be derived from the sequence often only unreliably, if at all. The −10 region, too, is less highly conserved than in organisms comprising AT-rich genomes. Consensus sequences which have previously been described here are TGNGNTA(C/T)AATGG and GNTANAATNG (Patek et al (2003), J. of Biotechnology 104, 311-323; Vasicova et al. (1999) J. Bacteriol 181, 6188-6191).
It is well known that a high variability generally occurs in the consensus sequences of bacteria having a moderate or high GC content (such as, for example, C. glutamicum ) (Bourn and Babb, 1995., Bashyam et al, 1996). This applies in particular to the −35 region which therefore frequently cannot be predicted. This is described, for example, in Patek et al (2003). J of Biotechnology 104, 325-334.
b) Expression Cassettes
The invention further relates to the multiple promoter-comprising expression units of the invention, functionally linked to a translatable nucleic acid sequence. These constructs which are capable of expressing genes are, for the purposes of the invention, also referred to as expression cassettes.
A “functional linkage” means in this connection, for example, the sequential arrangement of any of the expression units of the invention and a nucleic acid sequence to be expressed transgenically and, if appropriate, further regulatory elements such as a terminator, for example, so as for each of said regulatory elements to be able to fulfill a function in transgenic expression of said nucleic acid sequence. This does not necessarily require a direct linkage in the chemical sense. Genetic control sequences such as, for example, enhancer sequences can exert their function on the target sequence also from more distant positions or even from other DNA molecules. Preference is given to arrangements in which the nucleic acid sequence to be expressed transgenically is positioned behind (i.e. at the 3′ end of) the expression unit sequence of the invention, so that the two sequences are covalently connected to one another. The distance between the expression unit sequence and the nucleic acid sequence to be expressed transgenically is here preferably less than 200 base pairs, particularly preferably less than 100 base pairs, very particularly preferably less than 50 base pairs.
The multiple promoter-comprising expression cassettes of the invention make it possible to achieve an expression activity which is different in comparison with the original expression activity and thus to fine-regulate expression of the desired gene.
Regulation of the expression of genes in the microorganism by expression units of the invention is preferably achieved by
introducing one or more expression units of the invention, if appropriate with altered specific expression activity, into the genome of the microorganism so that one or more endogenous genes are expressed under the control of the introduced expression units of the invention; or introducing one or more nucleic acid constructs comprising an expression unit of the invention, if appropriate with altered specific expression activity, and, functionally linked, one or more nucleic acids to be expressed, i.e. an expression cassette, into the microorganism.

In the expression cassettes of the invention, the physical position of the expression unit relative to the gene to be expressed is chosen so as for said expression unit to regulate transcription, and preferably also translation, of the gene to be expressed and thus to enable the formation of one or more proteins. “To enable the formation” here includes to constitutively increase said formation, to attenuate or block said formation under specific conditions and/or to increase said formation under specific conditions. The “conditions” here comprise: (1) adding a component to the culture medium, (2) removing a component from the culture medium, (3) replacing a component in the culture medium with a second component, (4) increasing the temperature of the culture medium, (5) reducing the temperature of the culture medium, and (6) regulating the atmospheric conditions such as, for example, oxygen concentration or nitrogen concentration, in which the culture medium is maintained.
c) General Information:
All of the abovementioned nucleic acids having promoter activity and expression units and expression cassettes may furthermore be prepared in a manner known per se by chemical synthesis from the nucleotide building blocks, for example by fragment condensation of individual overlapping complementary nucleic acid building blocks of the double helix. The chemical synthesis of oligonucleotides may be carried out, for example, in a manner known per using the phosphoamidite method (Voet, Voet, 2 nd edition, Wiley Press New York, pp. 896-897). The assembly of synthetic oligonucleotides and the filling-in of gaps with the aid of the Klenow fragment of DNA polymerase and ligation reactions and also general cloning processes are described in Sambrook et al. (1989), Molecular cloning: A laboratory manual, Cold Spring Harbor Laboratory Press.
The methods and techniques utilized for the present inventions are known to the skilled worker trained in microbiological and recombinant DNA techniques. Methods and techniques for growing bacterial cells, transporting isolated DNA molecules into the host cell and isolating, cloning and sequencing isolated nucleic acid molecules, etc., are examples of such techniques and methods. These methods are described in many items of the standard literature: Davis et al., Basic Methods In Molecular Biology (1986); J. H. Miller, Experiments in Molecular Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1972); J. H. Miller, A Short Course in Bacterial Genetics, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1992); M. Singer and P. Berg, Genes & Genomes, University Science Books, Mill Valley, Calif. (1991); J. Sambrook, E. F. Fritsch and T. Maniatis, Molecular Cloning: A Laboratory Manual, 2 nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989); P. B. Kaufmann et al., Handbook of Molecular and Cellular Methods in Biology and Medicine, CRC Press, Boca Raton, Fla. (1995); Methods in Plant Molecular Biology and Biotechnology, B. R. Glick and J. E. Thompson, eds., CRC Press, Boca Raton, Fla. (1993); and P. F. Smith-Keary, Molecular Genetics of Escherichia coli , The Guilford Press, New York, N.Y. (1989).
All nucleic acid molecules of the present invention are preferably in the form of an isolated nucleic acid molecule. An “isolated” nucleic acid molecule is removed from other nucleic acid molecules present in the natural source of said nucleic acid and may additionally be essentially free of other cellular material or culture medium, if prepared by recombinant techniques, or free of chemical precursors or other chemicals, if synthesized chemically.
The invention furthermore comprises the nucleic acid molecules complementary to the specifically described nucleotide sequences, or a section thereof.
The nucleotide sequences of the invention also enable probes and primers to be generated which can be used for identifying and/or cloning homologous sequences in other cell types and microorganisms. Probes and primers of this kind usually comprise a nucleotide sequence region which, under stringent conditions, hybridizes to at least about 12, preferably at least about 25, such as, for example, about 40, 50 or 75, continuous nucleotides of a sense strand of a nucleic acid sequence of the invention or of a corresponding antisense strand.
The invention also comprises those nucleic acid sequences which comprise “silent mutations” or which have been altered in comparison with a specifically mentioned sequence according to the codon usage of a special source or host organism, as well as naturally occurring variants, for example splice variants or allelic variants, thereof.
III. Expression Vectors
The invention also relates to expression vectors comprising an above-described expression cassette of the invention.
Vectors are well known to the skilled worker and can be found, for example, in “Cloning Vectors” (Pouwels P. H. et al., eds., Elsevier, Amsterdam-New York-Oxford, 1985). Apart from plasmids, vectors also mean any other vectors known to the skilled worker, such as, for example, phages, transposons, IS elements, plasmids, cosmids, and linear or circular DNA. Said vectors may be replicated autonomously in the host organism or may be replicated chromosomally.
Preferred plasmids are particularly preferably those which are replicated in coryneform bacteria. Numerous known plasmid vectors such as, for example, pZ1 (Menkel et al., Applied and Environmental Microbiology (1989) 64: 549-554), pEKEx1 (Eikmanns et al., Gene 102: 93-98 (1991)) or pHS2-1 (Sonnen et al., Gene 107: 69-74 (1991)) are based on the cryptic plasmids pHM1519, pBL1 or pGA1. Other plasmid vectors such as, for example, pCLiK5MCS (see Example 1) or those based on pCG4 (U.S. Pat. No. 4,489,160) or pNG2 (Serwold-Davis et al., FEMS Microbiology Letters 66, 119-124 (1990)) or pAG1 (U.S. Pat. No. 5,158,891) may be used in the same way.
Furthermore suitable are also those plasmid vectors with the aid of which the process of gene amplification by integration into the chromosome can be applied, as has been described, for example, by Remscheid et al. (Applied and Environmental Microbiology 60, 126-132 (1994)) for the duplication and amplification of the hom-thrB operon. In this method, the complete gene is cloned into a plasmid vector which can replicate in a host (typically E. coli ) but not in C. glutamicum . Examples of suitable vectors are pSUP301 (Simon et al., Bio/Technology 1, 784-791 (1983)), pK18mob or pK19mob (Schäfer et al., Gene 145, 69-73 (1994)), Bernard et al., Journal of Molecular Biology, 234: 534-541 (1993)), pEM1 (Schrumpf et al. 1991, Journal of Bacteriology 173: 4510-4516) or pBGS8 (Spratt et al., 1986, Gene 41: 337-342). The plasmid vector which contains the gene to be amplified is subsequently transferred by transformation into the desired C. glutamicum strain. Transformation methods are described, for example, in Thierbach et al. (Applied Microbiology and Biotechnology 29, 356-362 (1988)), Dunican and Shivnan (Biotechnology 7, 1067-1070 (1989)) and Tauch et al. (FEMS Microbiological Letters 123, 343-347 (1994)).
IV. Genes and Proteins Encoded Thereby
The rate of transcription and/or the rate of translation of genes in microorganisms, in comparison with the wild type, may be altered or caused by regulating the transcription of genes in said microorganism by expression units of the invention, said genes being heterologous with respect to said expression units.
This is preferably achieved by
introducing one or more nucleic acids of the invention having promoter activity into the genome of the microorganism so that one or more endogenous genes are transcribed under the control of said introduced nucleic acid having promoter activity, if appropriate having an altered specific promoter activity, or introducing one or more nucleic acid constructs containing a nucleic acid of the invention having promoter activity and, functionally linked, one or more exogenous or endogenous nucleic acids to be transcribed, into the microorganism.

If one or more genes are introduced into the genome of a microorganism so that one or more introduced genes are transcribed under the control of the nucleic acids of the invention, insertion may take place so as for the gene or the genes to be integrated into coding or noncoding regions. The insertion preferably takes place into noncoding regions.
In this connection, nucleic acid constructs may be inserted chromosomally or extrachromosomally. The nucleic acid constructs are preferably inserted chromosomally. A “chromosomal” integration is the insertion of a DNA fragment into the chromosome of a host cell.
“Endogenous” means genetic information such as, for example, genes, which is already present in the wild-type genome (as defined above).
“Exogenous” means genetic information such as, for example, genes, which is not present in the wild-type genome. If exogenous genetic information, for example the multiple promoter-comprising expression units of the invention, is introduced into the genome of a wild-type strain, thereby generating a genetically modified strain, then this genetic information is endogenous in a comparison of the initially generated genetic strain with its progeny, but exogenous in a comparison with the original wild-type strain which did not comprise said genetic information.
The term “genes” with respect to regulation of transcription by the nucleic acids of the invention having promoter activity means preferably nucleic acids which comprise a region to be transcribed, i.e., for example, a region regulating translation, a coding region and, if appropriate, further regulatory elements such as, for example, a terminator.
The term “genes” with respect to the regulation of expression by the expression units of the invention means preferably nucleic acids which comprise a coding region and, if appropriate, further regulatory elements such as, for example, a terminator.
A “coding region” means a nucleic acid sequence which encodes a protein.
“Heterologous” with respect to nucleic acids having promoter activity and genes means that the genes used are not transcribed in the wild type with regulation of the nucleic acids of the invention having promoter activity, but that a new functional linkage which does not occur in the wild type is produced, and the functional combination of nucleic acid of the invention having promoter activity and specific gene does not occur in the wild type.
“Heterologous” with respect to expression units and genes means that the genes used are not expressed in the wild type with regulation of the expression units of the invention, but that a new functional linkage which does not occur in the wild type is produced, and the functional combination of expression unit of the invention and specific gene does not occur in the wild type.
In a preferred embodiment of the above-described processes of the invention for altering or causing the rate of transcription and/or rate of expression of genes in microorganisms, the genes are selected from the group consisting of nucleic acids encoding a protein of the biosynthetic pathway of fine chemicals, it being possible for said genes to contain further regulatory elements, if appropriate.
In a particularly preferred embodiment of the above-described processes of the invention for altering or causing the rate of transcription and/or the rate of expression of genes in microorganisms, the genes are selected from among:
Nucleic acids encoding a protein of the biosynthetic pathway of proteinogenic and nonproteinogenic amino acids, nucleic acids encoding a protein of the biosynthetic pathway of nucleotides and nucleosides, nucleic acids encoding a protein of the biosynthetic pathway of organic acids, nucleic acids encoding a protein of the biosynthetic pathway of lipids and fatty acids, nucleic acids encoding a protein of the biosynthetic pathway of diols, nucleic acids encoding a protein of the biosynthetic pathway of carbohydrates, nucleic acids encoding a protein of the biosynthetic pathway of aromatic compounds, nucleic acids encoding a protein of the biosynthetic pathway of vitamins, nucleic acids encoding a protein of the biosynthetic pathway of cofactors, and nucleic acids encoding a protein of the biosynthetic pathway of enzymes, nucleic acids encoding a protein of the central metabolism, it being possible for the genes to contain further regulatory elements, if appropriate.
In a particularly preferred embodiment, the proteins are selected from the biosynthetic pathway of amino acids, namely:
aspartate kinase, aspartate semialdehyde dehydrogenase, diaminopimelate dehydrogenase, diaminopimelate decarboxylase, dihydrodipicolinate synthetase, dihydrodipicolinate reductase, glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, pyruvate carboxylase, triosephosphate isomerase, transcriptional regulator LuxR, transcriptional regulator LysR1, transcriptional regulator LysR2, malate-quinone oxidoreductase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, transketolase, transaldolase, homoserine O-acetyltransferase, cystathionine gamma-synthase, cystathionine beta-lyase, serine hydroxymethyltransferase, O-acetylhomoserine sulfhydrylase, methylenetetrahydrofolate reductase, phosphoserine aminotransferase, phosphoserine phosphatase, serine acetyltransferase, homoserine dehydrogenase, homoserine kinase, threonine synthase, threonine exporter carrier, threonine dehydratase, pyruvate oxidase, lysine exporter, biotin ligase, cysteine synthase I, cysteine synthase II, coenzyme B12-dependent methionine synthase, coenzyme B12-independent methionine synthase activity, sulfate adenylyltransferase subunits 1 and 2, phosphoadenosine phosphosulfate reductase, ferredoxin sulfite reductase, ferredoxin NADP reductase, 3-phosphoglycerate dehydrogenase, RXA00655 regulator, RXN2910 regulator, arginyl-tRNA synthetase, phosphoenolpyruvate carboxylase, threonine efflux protein, serine hydroxymethyltransferase, fructose 1,6-bisphosphatase, protein of sulfate reduction RXA077, protein of sulfate reduction RXA248, protein of sulfate reduction RXA247, OpcA protein, 1-phosphofructokinase, 6-phosphofructokinase, tetrahydropicolinate succinylase, succinyl aminoketopimelate aminotransferase, succinyl diaminopimelate desuccinylase, diaminopimelate epimerase, 6-phosphogluconate dehydrogenase, glucose-phosphate isomerase, phosphoglycerate mutase, enolase, pyruvate kinase, aspartate transaminase, and malate enzyme.
Preferred proteins and nucleic acids encoding said proteins are protein sequences and, respectively, nucleic acid sequences of microbial origin, preferably from bacteria of the genus Corynebacterium or Brevibacterium , preferably from coryneform bacteria, particularly preferably from Corynebacterium glutamicum.
Examples of particularly preferred protein sequences and of the corresponding nucleic acid sequences encoding said proteins of the biosynthetic pathway of amino acids, the document referring thereto, and the designation thereof in the reference document are listed in Table 1:








TABLE 1




Nucleic




acid

SEQ. ID. NO.


encoding
Reference
in reference

Protein
protein
document
document



Aspartate kinase
ask or
EP1108790
DNA: 281


lysC

Protein: 3781

Aspartate semialdehyde
asd
EP1108790
DNA: 331

dehydrogenase


Protein: 3831

Dihydrodipicolinate
dapA
WO 0100843
DNA: 55

synthetase


Protein: 56

Dihydrodipicolinate
dapB
WO 0100843
DNA: 35

reductase


Protein: 36

meso-Diaminopimelate
ddh
EP1108790
DNA: 3494

D-dehydrogenase


Protein: 6944

Diaminopicolinate
lysA
EP1108790
DNA: 3451

decarboxylase


Prot.: 6951

Lysine exporter
lysE
EP1108790
DNA: 3455




Prot.: 6955

Tetrahydropicolinate
dapD
EP1108790
DNA: 1229

succinylase


Prot: 4729

Succinyl
dapC
WO 0100843
DNA: 327

aminoketopimelate


Prot: 328

aminotransferase




Succinyl
dapE
WO 0100843
DNA: 31

diaminopimelate


Prot: 32

desuccinylase




Diaminopimelate
dapF
EP1108790
DNA: 2131

epimerase


Prot: 5632

Arginyl-tRNA synthetase
argS
EP1108790
DNA: 3450




Prot.: 6950

Glucose-6-phosphate
zwf
WO 0100844
DNA: 243

dehydrogenase


Prot.: 244

Transketolase
RXA2739
EP 1108790
DNA: 1740




Prot: 5240

Transaldolase
RXA2738
WO 0100844
DNA: 245




Prot: 246

OpcA
opcA
WO 0100804
DNA: 79




Prot: 80

6-Phospho-
RXA2735
WO 0100844
DNA: 1

gluconolactonase


Prot.: 2

6-Phosphogluconate

EP1108790
DNA: 1605

dehydrogenase


Prot: 5105

Glucosephosphate
gpi
WO 0100844
DNA: 41

isomerase


Prot.: 42

Malate-quinone
mqo
WO 0100844
DNA: 569

oxidoreductase


Protein: 570

Glyceraldehyde-3-
gap
WO 0100844
DNA: 187

phosphate


Prot.: 188

dehydrogenase




3-Phosphoglycerate
pgk
WO 0100844
DNA: 69

kinase


Prot.: 70

Triosephosphate
tpi
WO 0100844
DNA: 61

isomerase


Prot.: 62

1-Phosphofructokinase 1
pfk1
WO0100844
DNA: 55




Protein: 56

1-Phosphofructokinase 2
pfk2
WO0100844
DNA: 57




Protein: 58

6-Phosphofructokinase 1
6-pfk1
EP 1108790
DNA: 1383




Protein: 4883

6-Phosphofructokinase 2
6-pfk2
DE 10112992
DNA: 1




Protein: 2

Fructose-1,6-
fbr1
EP1108790
DNA: 1136

bisphosphatase 1


Protein: 4636

Pyruvate oxidase
poxB
WO 0100844
DNA: 85




Protein: 86

Phosphoglycerate mutase

WO 0100844
DNA: 49




Prot.: 50

Enolase
eno
WO 0100844
DNA: 71




Prot.: 72

Pyruvate kinase
pykA
WO 0100844
DNA: 75




Prot.: 76

Pyruvate kinase
pykA
EP1108790
DNA: 3328




Prot.: 6828

Pyruvate carboxylase
pycA
EP1108790
DNA: 765




Prot.: 4265

Aspartate transaminase

EP1108790
DNA: 3226




Prot: 4726




DNA: 3134




Prot.: 6634




DNA: 2861




Prot.: 6361




DNA: 911




Prot.: 4411

PEP-carboxylase
pck
EP1108790
DNA: 3470




Prot.: 6970

Malate enzyme
malE
EP1108790
DNA: 3328




Prot: 6828

Biotin ligase
birA
EP1108790
DNA: 786




Prot.: 4286

Homoserine kinase
thrB
WO 0100843
DNA: 173




Prot.: 174

Threonine synthase
thrC
WO 0100843
DNA: 175




Prot.: 176

Threonine export carrier
thrE
WO 0251231
DNA: 41




Prot.: 42

Threonine efflux protein
RXA2390
WO 0100843
DNA: 7




Prot.: 8

Threonine dehydratase
ilvA
EP 1108790
DNA: 2328




Prot.: 5828

Homoserine-O-
metA
EP 1108790
DNA: 727

acetyltransferase


Prot: 4227

Cystathionine gamma-
metB
EP 1108790
DNA: 3491

synthase


Prot: 6991

Cystathionine beta-lyase
metC
EP 1108790
DNA: 2535




Prot: 6035

Coenzyme B12-
metH
EP 1108790
DNA: 1663

dependent methionine


Prot: 5163

synthase




O-acetylhomoserine
metY
EP 1108790
DNA: 726

sulfhydrylase


Prot: 4226

Methylenetetrahydro-
metF
EP 1108790
DNA: 2379

folate reductase


Prot: 5879

D-3-Phosphoglycerate
serA
EP 1108790
DNA: 1415

dehydrogenase


Prot: 4915

Phosphoserine
serB
WO 0100843
DNA: 153

phosphatase 1


Prot.: 154

Phosphoserine
serB
EP 1108790
DNA: 467

phosphatase 2


Prot: 3967

Phosphoserine
serB
EP 1108790
DNA: 334

phosphatase 3


Prot.: 3834

Phosphoserine
serC
WO 0100843
DNA: 151

aminotransferase


Prot.: 152

Serine acetyl transferase
cysE
WO 0100843
DNA: 243




Prot.: 244

Cysteine synthase I
cysK
EP 1108790
DNA: 2817




Prot.: 6317

Cysteine synthase II
CysM
EP 1108790
DNA: 2338




Prot.: 5838

Homoserine
hom
EP 1108790
DNA: 3452

dehydrogenase


Prot.: 6952

Coenzyme B12-
metE
WO 0100843
DNA: 755

independent methionine


Prot.: 756

synthase




Serine hydroxymethyl
glyA
WO 0100843
DNA: 143

transferase


Prot.: 144

Protein in sulfate
RXA247
EP 1108790
DNA: 3089

reduction


Prot.: 6589

Protein in sulfate
RXA248
EP 1108790
DNA: 3090

reduction


Prot.: 6590

Sulfate
CysN
EP 1108790
DNA: 3092

adenylyltransferase


Prot.: 6592

subunit 1




Sulfate
CysD
EP 1108790
DNA: 3093

adenylyltransferase


Prot.: 6593

subunit 2




Phosphoadenosine
CysH
WO 02729029
DNA: 7

phosphosulfate reductase


Prot.: 8

Ferredoxin sulfite
RXA073
WO 0100842
DNA: 329

reductase


Prot.: 330

Ferredoxin NADP
RXA076
WO 0100843
DNA: 79

reductase


Prot.: 80

Transcriptional regulator
luxR
WO 0100842
DNA: 297

LuxR


Protein: 298

Transcriptional regulator
lysR1
EP 1108790
DNA: 676

LysR1


Protein: 4176

Transcriptional regulator
lysR2
EP 1108790
DNA: 3228

LysR2


Protein: 6728

Transcriptional regulator
lysR3
EP 1108790
DNA: 2200

LysR3


Protein: 5700

RXA00655 regulator
RXA655
US2003162267.2
DNA: 1




Prot.: 2

RXN02910 regulator
RXN2910
US2003162267.2
DNA: 5




Prot.: 6


















































































































































































Preference is given to selecting the target gene G to be regulated according to the invention from the genes listed above.
Further particularly preferred protein sequences from the biosynthetic pathway of amino acids have in each case the amino acid sequence indicated in Table 1 for this protein, where the respective protein has, in at least one of the amino acid positions indicated in Table 2, column 2 for this amino acid sequence, a different proteinogenic amino acid than the respective amino acid indicated in Table 2, column 3 in the same line. In a further preferred embodiment, the proteins have, in at least one of the amino acid positions indicated in Table 2, column 2 for the amino acid sequence, the amino acid indicated in Table 2, column 4 in the same line. The proteins indicated in Table 2 are mutated proteins of the biosynthetic pathway of amino acids, which have particularly advantageous properties and are therefore particularly suitable for expressing the corresponding nucleic acids through a promoter construct of the invention and for producing amino acids. For example, the mutation T311I leads to the feedback inhibition of ask being switched off.
The corresponding nucleic acids which encode a mutated protein described above from Table 2 can be prepared by conventional methods.
A suitable starting point for preparing the nucleic acid sequences encoding a mutated protein is, for example, the genome of a Corynebacterium glutamicum strain which is obtainable from the American Type Culture Collection under the designation ATCC 13032, or the nucleic acid sequences referred to in Table 1. For the back-translation of the amino acid sequence of the mutated proteins into the nucleic acid sequences encoding these proteins, it is advantageous to use the codon usage of the organism into which the nucleic acid sequence is to be introduced or in which the nucleic acid sequence is present. For example, it is advantageous to use the codon usage of Corynebacterium glutamicum for Corynebacterium glutamicum . The codon usage of the particular organism can be ascertained in a manner known per se from databases or patent applications which describe at least one protein and one gene which encodes this protein from the desired organism.
The information in Table 2 is to be understood in the following way:
In column 1 “identification”, an unambiguous designation for each sequence in relation to Table 1 is indicated.
In column 2 “AA-POS”, the respective number refers to the amino acid position of the corresponding polypeptide sequence from Table 1. A “26” in the column “AA-POS” accordingly means amino acid position 26 of the correspondingly indicated polypeptide sequence. The numbering of the position starts at +1 at the N terminus.
In column 3 “AA wild type”, the respective letter designates the amino acid—represented in one-letter code—at the position indicated in column 2 in the corresponding wild-type strain of the sequence from Table 1.
In column 4 “AA mutant”, the respective letter designates the amino acid—represented in one-letter code—at the position indicated in column 2 in the corresponding mutant strain.
In column 5 “function”, the physiological function of the corresponding polypeptide sequence is indicated.
For a mutated protein with a particular function (column 5) and a particular initial amino acid sequence (Table 1), columns 2, 3 and 4 describe at least one mutation, and a plurality of mutations for some sequences. This plurality of mutations always refers to the closest initial amino acid sequence above in each case (Table 1). The term “at least one of the amino acid positions” of a particular amino acid sequence preferably means at least one of the mutations described for this amino acid sequence in columns 2, 3 and 4.
One-letter code for proteinogenic amino acids:










A
alanine


C
cysteine


D
aspartate


E
glutamate


F
phenylalanine


G
glycine


H
histidine


I
isoleucine


K
lysine


L
leucine


M
methionine


N
asparagine


P
proline


Q
glutamine


R
arginine


S
serine


T
threonine


V
valine


W
tryptophan


Y
tyrosine

































TABLE 2 Column 1 Column 2 Column 3 Column 4 Column 5 Identification AA position AA wild type AA mutant Function ask 317 S A aspartate kinase 311 T I 279 A T asd 66 D G aspartate-semialdehyde dehydrogenase 234 R H 272 D E 285 K E 20 L F dapA 2 S A dihydrodipicolinate synthetase 84 K N 85 L V dapB 91 D A dihydrodipicolinate reductase 83 D N ddh 174 D E meso-diaminopimelate D-dehydrogenase 235 F L 237 S A lysA 265 A D diaminopicolinate decarboxylase 320 D N 332 I V argS 355 G D arginyl-tRNA synthetase 156 A S 513 V A 540 H R zwf 8 S T glucose-6-phosphate dehydrogenase 150 T A 321 G S gap 264 G S glyceraldehyde-3-phosphate dehydrogenase pycA 7 S L pyruvate carboxylase 153 E D 182 A S 206 A S 227 H R 455 A G 458 P S 639 S T 1008 R H 1059 S P 1120 D E pck 162 H Y PEP carboxylase 241 G D 829 T R thrB 103 S A homoserine kinase 190 T A 133 A V 138 P S thrC 69 G R threonine synthase 478 T I RXA330 85 I M threonine efflux protein 161 F I 195 G D hom 104 V I homoserine dehydrogenase 116 T I 148 G A 59 V A 270 T S 345 R P 268 K N 61 D H 72 E Q lysR1 80 R H transcriptional regulator LysR1 lysR3 142 R W transcriptional regulator LysR3 179 A T RXA2739 75 N D transketolase 329 A T 332 A T 556 V I RXA2738 242 K M transaldolase opcA 107 Y H OpcA 219 K N 233 P S 261 Y H 312 S F 65 G R aspartate-1-decarboxylase 33 G S 6-phosphogluconolactonase
Genetically Modified Microorganisms

The expression units of the invention, the above-described genes and the above-described nucleic acid constructs or expression cassettes are introduced into the microorganism, in particular into coryneform bacteria, by methods known to the skilled worker, such as conjugation or electroporation (for example, Liebl et al (1989) FEMS Microbiology Letters 53, 299-303).
Integrated expression cassettes are preferably selected by the SacB method. The SacB method is known to the skilled worker and is described, for example, in Schäfer A, Tauch A, Jäger W. Kalinowski J. Thierbach G. Pühler A.; Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum , Gene. 1994 Jul. 22; 145(1):69-73 and Blomfield I C, Vaughn V, Rest R F, Eisenstein B I.; Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon; Mol. Microbiol. 1991 June; 5(6):1447-57.
Preference is given to using according to the invention as starting microorganisms those which already produce the desired variable product (fine chemical/protein).
The invention therefore also relates to a genetically modified microorganism which comprises an expression cassette of the invention or a vector comprising the expression cassette of the invention.
The present invention particularly preferably relates to genetically modified microorganisms, in particular coryneform bacteria, which comprise a vector, in particular a shuttle vector or plasmid vector, which carries at least one expression cassette of the invention.
Preferred microorganisms or genetically modified microorganisms are bacteria, algae, fungi or yeasts.
Particularly preferred microorganisms are, in particular, coryneform bacteria.
Preferred coryneform bacteria are bacteria of the genus Corynebacterium , in particular of the species Corynebacterium glutamicum, Corynebacterium acetoglutamicum, Corynebacterium acetoacidophilum, Corynebacterium thermoaminogenes, Corynebacterium melassecola and Corynebacterium efficiens or of the genus Brevibacterium , in particular of the species Brevibacterium flavum, Brevibacterium lactofermentum and Brevibacterium divaricatum.
Particularly preferred bacteria of the genera Corynebacterium and Brevibacterium are selected from the group consisting of Corynebacterium glutamicum ATCC 13032, Corynebacterium acetoglutamicum ATCC 15806, Corynebacterium acetoacidophilum ATCC 13870, Corynebacterium thermoaminogenes FERM BP-1539, Corynebacterium melassecola ATCC 17965, Corynebacterium efficiens DSM 44547, Corynebacterium efficiens DSM 44548. Corynebacterium efficiens DSM 44549, Brevibacterium flavum ATCC 14067, Brevibacterium lactofermentum ATCC 13869, Brevibacterium divaricatum ATCC 14020, Corynebacterium glutamicum KFCC10065 and Corynebacterium glutamicum ATCC 21608, and also strains derived therefrom, for example by classical mutation and selection or by directed mutation.
The abbreviation KFCC means the Korean Federation of Culture Collection, the abbreviation ATCC means the American type strain culture collection, and the abbreviation DSM (or DSMZ) means the Deutsche Sammlung von Mikroorganismen (Deutsche Sammlung von Mikroorganismen and Zellkulturen).
Further particularly preferred bacteria of the genera Corynebacterium and Brevibacterium are listed in Table 3:






TABLE 3



Bacterium
Deposition number












Genus
species
ATCC
FERM
NRRL
CECT
NCIMB
CBS
NCTC
DSMZ















Brevibacterium


ammoniagenes

21054









Brevibacterium


ammoniagenes

19350









Brevibacterium


ammoniagenes

19351









Brevibacterium


ammoniagenes

19352









Brevibacterium


ammoniagenes

19353









Brevibacterium


ammoniagenes

19354









Brevibacterium


ammoniagenes

19355









Brevibacterium


ammoniagenes

19356









Brevibacterium


ammoniagenes

21055









Brevibacterium


ammoniagenes

21077









Brevibacterium


ammoniagenes

21553









Brevibacterium


ammoniagenes

21580









Brevibacterium


ammoniagenes

39101









Brevibacterium


butanicum

21196









Brevibacterium


divaricatum

21792
P928








Brevibacterium


flavum

21474









Brevibacterium


flavum

21129









Brevibacterium


flavum

21518









Brevibacterium


flavum



B11474







Brevibacterium


flavum



B11472







Brevibacterium


flavum

21127









Brevibacterium


flavum

21128









Brevibacterium


flavum

21427









Brevibacterium


flavum

21475









Brevibacterium


flavum

21517









Brevibacterium


flavum

21528









Brevibacterium


flavum

21529









Brevibacterium


flavum



B11477







Brevibacterium


flavum



B11478







Brevibacterium


flavum

21127









Brevibacterium


flavum



B11474







Brevibacterium


healii

15527









Brevibacterium


ketoglutamicum

21004









Brevibacterium


ketoglutamicum

21089









Brevibacterium


ketosoreductum

21914









Brevibacterium


lactofermentum




70






Brevibacterium


lactofermentum




74






Brevibacterium


lactofermentum




77






Brevibacterium


lactofermentum

21798









Brevibacterium


lactofermentum

21799









Brevibacterium


lactofermentum

21800









Brevibacterium


lactofermentum

21801









Brevibacterium


lactofermentum



B11470







Brevibacterium


lactofermentum



B11471







Brevibacterium


lactofermentum

21086









Brevibacterium


lactofermentum

21420









Brevibacterium


lactofermentum

21086









Brevibacterium


lactofermentum

31269









Brevibacterium


linens

9174









Brevibacterium


linens

19391









Brevibacterium


linens

8377









Brevibacterium


paraffinolyticum





11160





Brevibacterium

spec.





717.73




Brevibacterium

spec.





717.73




Brevibacterium

spec.
14604









Brevibacterium

spec.
21860









Brevibacterium

spec.
21864









Brevibacterium

spec.
21865









Brevibacterium

spec.
21866









Brevibacterium

spec.
19240









Corynebacterium


acetoacidophilum

21476









Corynebacterium


acetoacidophilum

13870









Corynebacterium


acetoglutamicum



B11473







Corynebacterium


acetoglutamicum



B11475







Corynebacterium


acetoglutamicum

15806









Corynebacterium


acetoglutamicum

21491









Corynebacterium


acetoglutamicum

31270









Corynebacterium


acetophilum



B3671







Corynebacterium


ammoniagenes

6872





2399



Corynebacterium


ammoniagenes

15511









Corynebacterium


fujiokense

21496









Corynebacterium


glutamicum

14067









Corynebacterium


glutamicum

39137









Corynebacterium


glutamicum

21254









Corynebacterium


glutamicum

21255









Corynebacterium


glutamicum

31830









Corynebacterium


glutamicum

13032









Corynebacterium


glutamicum

14305









Corynebacterium


glutamicum

15455









Corynebacterium


glutamicum

13058









Corynebacterium


glutamicum

13059









Corynebacterium


glutamicum

13060









Corynebacterium


glutamicum

21492









Corynebacterium


glutamicum

21513









Corynebacterium


glutamicum

21526









Corynebacterium


glutamicum

21543









Corynebacterium


glutamicum

13287









Corynebacterium


glutamicum

21851









Corynebacterium


glutamicum

21253









Corynebacterium


glutamicum

21514









Corynebacterium


glutamicum

21516









Corynebacterium


glutamicum

21299









Corynebacterium


glutamicum

21300









Corynebacterium


glutamicum

39684









Corynebacterium


glutamicum

21488









Corynebacterium


glutamicum

21649









Corynebacterium


glutamicum

21650









Corynebacterium


glutamicum

19223









Corynebacterium


glutamicum

13869









Corynebacterium


glutamicum

21157









Corynebacterium


glutamicum

21158









Corynebacterium


glutamicum

21159









Corynebacterium


glutamicum

21355









Corynebacterium


glutamicum

31808









Corynebacterium


glutamicum

21674









Corynebacterium


glutamicum

21562









Corynebacterium


glutamicum

21563









Corynebacterium


glutamicum

21564









Corynebacterium


glutamicum

21565









Corynebacterium


glutamicum

21566









Corynebacterium


glutamicum

21567









Corynebacterium


glutamicum

21568









Corynebacterium


glutamicum

21569









Corynebacterium


glutamicum

21570









Corynebacterium


glutamicum

21571









Corynebacterium


glutamicum

21572









Corynebacterium


glutamicum

21573









Corynebacterium


glutamicum

21579









Corynebacterium


glutamicum

19049









Corynebacterium


glutamicum

19050









Corynebacterium


glutamicum

19051









Corynebacterium


glutamicum

19052









Corynebacterium


glutamicum

19053









Corynebacterium


glutamicum

19054









Corynebacterium


glutamicum

19055









Corynebacterium


glutamicum

19056









Corynebacterium


glutamicum

19057









Corynebacterium


glutamicum

19058









Corynebacterium


glutamicum

19059









Corynebacterium


glutamicum

19060









Corynebacterium


glutamicum

19185









Corynebacterium


glutamicum

13286









Corynebacterium


glutamicum

21515









Corynebacterium


glutamicum

21527









Corynebacterium


glutamicum

21544









Corynebacterium


glutamicum

21492









Corynebacterium


glutamicum



B8183







Corynebacterium


glutamicum



B8182







Corynebacterium


glutamicum



B12416







Corynebacterium


glutamicum



B12417







Corynebacterium


glutamicum



B12418







Corynebacterium


glutamicum



B11476







Corynebacterium


glutamicum

21608









Corynebacterium


lilium


P973








Corynebacterium


nitrilophilus

21419



11594





Corynebacterium

spec.

P4445








Corynebacterium

spec.

P4446








Corynebacterium

spec.
31088









Corynebacterium

spec.
31089









Corynebacterium

spec.
31090









Corynebacterium

spec.
31090









Corynebacterium

spec.
31090









Corynebacterium

spec.
15954






20145


Corynebacterium

spec.
21857









Corynebacterium

spec.
21862









Corynebacterium

spec.
21863



The abbreviations have the following meaning:

ATCC: American Type Culture Collection, Rockville, MD, USA

FERM: Fermentation Research Institute, Chiba, Japan

NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA

CECT: Coleccion Espanola de Cultivos Tipo, Valencia, Spain

NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK

CBS: Centraalbureau voor Schimmelcultures, Baarn, NL

NCTC: National Collection of Type Cultures, London, UK

DSMZ: Deutsche Sammlung von Mikroorganismen and Zellkulturen, Braunschweig, Germany


























































































































































































Particular preference is given here to microorganisms of bacteria of the genus Corynebacterium , in particular those which are already capable of producing L-lysine, L-methionine and/or L-threonine. These are in particular coryne bacteria in which, for example, the gene coding for an aspartate kinase (ask gene) is deregulated or feedback inhibition has been eliminated or reduced. For example, such bacteria have a mutation in the ask gene, which results in a reduction or elimination of feedback inhibition, such as, for example, the mutation T311I.
The expression units of the invention enable the metabolic pathways to specific biosynthetic products in the above-described genetically modified microorganisms of the invention to be regulated.
For this purpose, for example, metabolic pathways which result in a specific biosynthetic product are enhanced by causing or increasing the rate of transcription or the rate of expression of genes of this biosynthetic pathway in which the increased amount of protein results in an increased total activity of these proteins of the desired biosynthetic pathway and thus in an enhanced metabolic flux toward the desired biosynthetic product.
Furthermore, metabolic pathways which diverge from a specific biosynthetic product may be attenuated by reducing the rate of transcription or rate of expression of genes of this diverging biosynthetic pathway in which the reduced amount of protein results in a reduced total activity of these proteins of the unwanted biosynthetic pathway and thus additionally in an enhanced metabolic flux toward the desired biosynthetic product.
The genetically modified microorganisms of the invention are capable, for example, of producing biosynthetic products from glucose, sucrose, lactose, fructose, maltose, molasses, starch, cellulose or from glycerol and ethanol.
The invention therefore relates to a process for producing biosynthetic products by culturing genetically modified microorganisms of the invention.
Depending on the desired biosynthetic product, the rate of transcription or rate of expression of various genes must be increased or reduced. It is usually advantageous to alter the rate of transcription or rate of expression of a plurality of genes, i.e. to increase the rate of transcription or rate of expression of a combination of genes and/or to reduce of the rate of transcription or rate of expression of a combination of genes.
In the genetically modified microorganisms of the invention, at least one altered, i.e. increased or reduced, rate of transcription or rate of expression of a gene can be attributed to a nucleic acid of the invention having promoter activity or to an expression unit of the invention.
Further, additionally altered, i.e. additionally increased or additionally reduced, rates of transcription or rates of expression of further genes in the genetically modified microorganism may, but need not, derive from the nucleic acids of the invention having promoter activity or the expression units of the invention.
The invention therefore furthermore relates to a process for producing biosynthetic products by culturing genetically modified microorganisms of the invention.
V. Fields of Application of the Invention
The multiple promoter-comprising expression units, expression cassettes and vectors of the invention can, for example by used particularly advantageously in improved processes for fermentation production of biosynthetic products as described below.
The multiple promoter-comprising expression units of the invention or expression cassettes comprising them have the particular advantage of allowing a modulation of the expression of the functionally linked structural gene.
The expression units of the invention or expression cassettes comprising them may be used for altering, i.e. increasing or reducing, or for causing the rate of expression of genes in microorganisms, in comparison with the wild type. This provides the possibility, in the case of an increase in the rate of expression, of maximizing expression of the gene in question. By choosing a suitable expression unit, however, expression may also be at a strength which is below the maximum obtainable by a different expression unit, but which at the same time delivers the expression product in an amount more compatible for the expressing microorganism. This is particularly advantageous if expression product concentrations which are too high are toxic for the expressing microorganism. By way of selection from among expression units with in each case different strength of expression, the expression units of the invention thus enable expression to be fine-regulated to an optimal value, in particular for long-term expression.
The expression units of the invention or expression cassettes comprising said expression units may also be used for regulating and enhancing the formation of various biosynthetic products such as, for example, fine chemicals, proteins, in particular amino acids, in microorganisms, in particular in Corynebacterium species.
The invention therefore relates to the use of a multiple promoter-comprising expression unit of the invention for regulating a product biosynthesis. Here, the gene of a protein involved in the regulation of a product biosynthesis is put under the control of such an expression unit. Said product biosynthesis is influenced depending on the rate of transcription of the selected, multiple promoter-comprising expression unit. Alternatively, the gene of a protein involved in the regulation of a product biosynthesis may be expressed as constituent of an expression cassette of the invention in a microorganism and said product biosynthesis may be regulated by the protein expressed thereby. If the activity of a multiple promoter is weaker than that of the endogenous promoter, a multiple promoter may also be employed in order to attenuate specifically unwanted biosynthetic pathways.
The rate of transcription of a multiple promoter-comprising expression unit of the invention may furthermore be regulated by specific mutation of one or more individual promoters which constitute said multiple promoter. An increased or reduced promoter activity may be achieved by replacing nucleotides in the binding site of the RNA polymerase holoenzyme binding sites (known to the skilled worker also as −10 region and −35 region). An influence may furthermore be exerted by reducing or extending the distance between the RNA polymerase holoenzyme binding sites described by nucleotide deletions or nucleotide insertions; furthermore, by putting binding sites (also known as operators to the skilled worker) for regulatory proteins (known as repressors and activators to the skilled worker) in spatial proximity to the binding sites of the RNA polymerase holoenzyme, so that these regulators, after binding to a promoter sequence, attenuate or enhance binding and transcriptional activity of the RNA polymerase holoenzyme or else subject said binding and transcriptional activity to a new regulatory influence. Translational activity may also be influenced by mutating the ribosome binding-mediating, 3′-terminal sequence section of a single promoter P y within the multiple promoter-comprising expression unit of the invention.
VI. Biosynthetic Products and Preferred Production Processes
Preferred biosynthetic products prepared according to the invention are fine chemicals.
The term “fine chemical” is familiar to the skilled worker and includes compounds which are produced by an organism and are used in various branches of industry such as, for example but not restricted to, the pharmaceutical industry, the agriculture, cosmetics, food and feed industries. These compounds comprise organic acids such as, for example, lactic acid, succinic acid, tartaric acid, itaconic acid and diaminopimelic acid, and proteinogenic and nonproteinogenic amino acids, purine bases and pyrimidine bases, nucleosides and nucleotides (as described for example in Kuninaka, A. (1996) Nucleotides and related compounds, pp. 561-612, in Biotechnology vol. 6, Rehm et al., editors, VCH: Weinheim and the references present therein), lipids, saturated and unsaturated fatty acids (e.g. arachidonic acid), diols (e.g. propanediol and butanediol), carbohydrates (e.g. hyaluronic acid and trehalose), aromatic compounds (e.g. aromatic amines, vanillin and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, “Vitamins”, pp. 443-613 (1996) VCH: Weinheim and the references present therein; and Ong, A. S., Niki, E. and Packer, L. (1995) “Nutrition, Lipids, Health and Disease” Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia and the Society for Free Radical Research—Asia, held on Sep. 1-3, 1994 in Penang, Malaysia, AOCS Press (1995)), enzymes and all other chemicals described by Gutcho (1983) in Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and the references indicated therein.
Particularly preferred biosynthetic products are selected from the group of organic acids, proteins, nucleotides and nucleosides, both proteinogenic and nonproteinogenic amino acids, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, enzymes and proteins.
Preferred organic acids are lactic acid, succinic acid, tartaric acid, itaconic acid and diaminopimelic acid.
Preferred nucleosides and nucleotides are described for example in Kuninaka, A. (1996) Nucleotides and related compounds, pp. 561-612, in Biotechnology, vol. 6, Rehm et al., editors VCH: Weinheim and references present therein.
Preferred biosynthetic products are additionally lipids, saturated and unsaturated fatty acids such as, for example, arachidonic acid, diols such as, for example, propanediol and butanediol, carbohydrates such as, for example, hyaluronic acid and trehalose, aromatic compounds such as, for example, aromatic amines, vanillin and indigo, vitamins and cofactors as described for example in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, “Vitamins”, pp. 443-613 (1996) VCH: Weinheim and the references present therein; and Ong, A. S., Niki, E. and Packer, L. (1995) “Nutrition, Lipids, Health and Disease” Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia and the Society for Free Radical Research—Asia, held on Sep. 1-3, 1994 in Penang, Malaysia, AOCS Press (1995)), enzymes, polyketides (Cane et al. (1998) Science 282: 63-68) and all other chemicals described by Gutcho (1983) in Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and the references indicated therein.
Particularly preferred biosynthetic products are amino acids, particularly preferably essential amino acids, in particular L-glycine, L-alanine, L-leucine, L-methionine, L-phenylalanine, L-tryptophan, L-lysine, L-glutamine, L-glutamic acid, L-serine, L-proline, L-valine, L-isoleucine, L-cysteine, L-tyrosine, L-histidine, L-arginine, L-asparagine, L-aspartic acid and L-threonine, L-homoserine, especially L-lysine, L-methionine and L-threonine. An amino acid such as, for example, lysine, methionine and threonine means hereinafter both in each case the L and the D form of the amino acid, preferably the L form, i.e. for example L-lysine, L-methionine and L-threonine.
The following sections describe the particularly preferred preparations of lysine, methionine and threonine in more detail.
a) Preparation of Lysine
The invention relates in particular to a process for producing lysine by culturing genetically modified microorganisms with increased or caused expression rate of at least one gene compared with the wild type, where
the expression activity in the microorganism of at least one endogenous gene is regulated by an expression unit of the invention, or the expression of at least one gene in the microorganism is caused or altered by introducing into said microorganism an expression cassette of the invention comprising said gene.

The genes are selected here in particular from among nucleic acids encoding an aspartate kinase, nucleic acids encoding an aspartate-semialdehyde dehydrogenase, nucleic acids encoding a diaminopimelate dehydrogenase, nucleic acids encoding a diaminopimelate decarboxylase, nucleic acids encoding a dihydrodipicolinate synthetase, nucleic acids encoding a dihydrodipicolinate reductase, nucleic acids encoding a glyceraldehyde-3-phosphate dehydrogenase, nucleic acids encoding a 3-phosphoglycerate kinase, nucleic acids encoding a pyruvate carboxylase, nucleic acids encoding a triosephosphate isomerase, nucleic acids encoding a transcriptional regulator LuxR, nucleic acids encoding a transcriptional regulator LysR1, nucleic acids encoding a transcriptional regulator LysR2, nucleic acids encoding a malate-quinone oxidoreductase, nucleic acids encoding a glucose-6-phosphate dehydrogenase, nucleic acids encoding a 6-phosphogluconate dehydrogenase, nucleic acids encoding a transketolase, nucleic acids encoding a transaldolase, nucleic acids encoding a lysine exporter, nucleic acids encoding a biotin ligase, nucleic acids encoding an arginyl-tRNA synthetase, nucleic acids encoding a phosphoenolpyruvate carboxylase, nucleic acids encoding a fructose-1,6-bisphosphatase, nucleic acids encoding a protein OpcA, nucleic acids encoding a 1-phosphofructokinase, nucleic acids encoding a 6-phosphofructokinase, nucleic acids encoding a tetrahydropicolinate succinylase, nucleic acids encoding a succinyl aminoketopimelate aminotransferase, nucleic acids encoding a succinyl diaminopimelate desuccinylase, nucleic acids encoding a diaminopimelate epimerase, nucleic acids encoding a 6-phosphogluconate dehydrogenase, nucleic acids encoding a glucose phosphate isomerase, nucleic acids encoding a phosphoglycerate mutase, nucleic acids encoding an enolase, nucleic acids encoding a pyruvate kinase, nucleic acids encoding an aspartate transaminase and nucleic acids encoding a malate enzyme.
A further preferred embodiment of the process described above for preparing lysine comprises the genetically modified microorganisms, compared with the wild type, having additionally an increased activity, of at least one of the activities selected from among
aspartate kinase activity, aspartate semialdehyde dehydrogenase activity, diaminopimelate dehydrogenase activity, diaminopimelate decarboxylase activity, dihydrodipicolinate synthetase activity, dihydrodipicolinate reductase activity, glyceraldehyde-3-phosphate dehydrogenase activity, 3-phosphoglycerate kinase activity, pyruvate carboxylase activity, triosephosphate isomerase activity, activity of the transcriptional regulator LuxR, activity of the transcriptional regulator LysR1, activity of the transcriptional regulator LysR2, malate-quinone oxidoreductase activity, glucose-6-phosphate dehydrogenase activity, 6-phosphogluconate dehydrogenase activity, transketolase activity, transaldolase activity, lysine exporter activity, arginyl-tRNA synthetase activity, phosphoenolpyruvate carboxylase activity, fructose-1,6-bisphosphatase activity, protein OpcA activity, 1-phosphofructokinase activity, 6-phosphofructokinase activity, biotin ligase activity, and tetrahydropicolinate succinylase activity, succinyl aminoketopimelate aminotransferase activity, succinyl diaminopimelate desuccinylase activity, diaminopimelate epimerase activity, 6-phosphogluconate dehydrogenase activity, glucose phosphate isomerase activity, phosphoglycerate mutase activity, enolase activity, pyruvate kinase activity, aspartate transaminase activity and malate enzyme activity.
A further particularly preferred embodiment of the process described above for preparing lysine comprises the genetically modified microorganisms having, compared with the wild type, additionally a reduced activity, of at least one of the activities selected from the group of threonine dehydratase activity, homoserine O-acetyl-transferase activity, O-acetylhomoserine sulfhydrylase activity, phosphoenolpyruvate carboxykinase activity, pyruvate oxidase activity, homoserine kinase activity, homoserine dehydrogenase activity, threonine exporter activity, threonine efflux protein activity, asparaginase activity, aspartate decarboxylase activity and threonine synthase activity.
b) Preparation of Methionine
The invention further relates to a process for preparing methionine by culturing genetically modified microorganisms with increased or caused expression rate of at least one gene compared with the wild type, where
the expression activity in the microorganism of at least one endogenous gene is regulated by an expression unit of the invention, or the expression of at least one gene in the microorganism is caused or altered by introducing into said microorganism an expression unit of the invention comprising said gene.

The genes are selected here in particular from nucleic acids encoding an aspartate kinase, nucleic acids encoding an aspartate semialdehyde dehydrogenase, nucleic acids encoding a homoserine dehydrogenase, nucleic acids encoding a glyceraldehyde-3-phosphate dehydrogenase, nucleic acids encoding a 3-phosphoglycerate kinase, nucleic acids encoding a pyruvate carboxylase, nucleic acids encoding a triosephosphate isomerase, nucleic acids encoding a homoserine O-acetyltransferase, nucleic acids encoding a cystathionine gamma-synthase, nucleic acids encoding a cystathionine beta-lyase, nucleic acids encoding a serine hydroxymethyltransferase, nucleic acids encoding an O-acetylhomoserine sulfhydrylase, nucleic acids encoding a methylenetetrahydrofolate reductase, nucleic acids encoding a phosphoserine aminotransferase, nucleic acids encoding a phosphoserine phosphatase, nucleic acids encoding a serine acetyltransferase, nucleic acids encoding a cysteine synthase activity I, nucleic acids encoding a cysteine synthase activity II, nucleic acids encoding a coenzyme B12-dependent methionine synthase activity, nucleic acids encoding a coenzyme B12-independent methionine synthase activity, nucleic acids encoding a sulfate adenylyltransferase activity, nucleic acids encoding a phosphoadenosine phosphosulfate reductase activity, nucleic acids encoding a ferredoxin sulfite reductase activity, nucleic acids encoding a ferredoxin NADPH reductase activity, nucleic acids encoding a ferredoxin activity, nucleic acids encoding a protein of sulfate reduction RXA077, nucleic acids encoding a protein of sulfate reduction RXA248, nucleic acids encoding a protein of sulfate reduction RXA247, nucleic acids encoding an RXA0655 regulator and nucleic acids encoding an RXN2910 regulator, nucleic acids encoding a 6-phosphogluconate dehydrogenase, glucose phosphate isomerase, phosphoglycerate mutase, enolase, pyruvate kinase, aspartate transaminase or malate enzyme.
A further preferred embodiment of the process described above for preparing methionine comprises the genetically modified microorganisms having, compared with the wild type, additionally an increased activity of at least one of the activities selected from the group of aspartate kinase activity, aspartate semialdehyde dehydrogenase activity, homoserine dehydrogenase activity, glyceraldehyde-3-phosphate dehydrogenase activity, 3-phosphoglycerate kinase activity, pyruvate carboxylase activity, triosephosphate isomerase activity, homoserine O-acetyltransferase activity, cystathionine gamma-synthase activity, cystathionine beta-lyase activity, serine hydroxymethyltransferase activity, O-acetylhomoserine sulfhydrylase activity, methylenetetrahydrofolate reductase activity, phosphoserine aminotransferase activity, phosphoserine phosphatase activity, serine acetyltransferase activity, cysteine synthase I activity, cysteine synthase II activity, coenzyme B12-dependent methionine synthase activity, coenzyme B12-independent methionine synthase activity, sulfate adenylyltransferase activity, phosphoadenosine phosphosulfate reductase activity, ferredoxin sulfite reductase activity, ferredoxin NADPH reductase activity, ferredoxin activity, activity of a protein of sulfate reduction RXA077, activity of a protein of sulfate reduction RXA248, activity of a protein of sulfate reduction RXA247, activity of an RXA655 regulator and activity of an RXN2910 regulator, activity of a 6-phosphogluconate dehydrogenase, activity of a glucose phosphate isomerase, activity of a phosphoglycerate mutase, activity of an enolase, activity of a pyruvate kinase, activity of an aspartate transaminase and activity of the malate enzyme.
A further particularly preferred embodiment of the method described above for preparing methionine comprises the genetically modified microorganisms having, compared with the wild type, additionally a reduced activity of at least one of the activities selected from the group of homoserine kinase activity, threonine dehydratase activity, threonine synthase activity, meso-diaminopimelate D-dehydrogenase activity, phosphoenolpyruvate carboxykinase activity, pyruvate oxidase activity, dihydrodipicolinate synthase activity, dihydrodipicolinate reductase activity and diaminopicolinate decarboxylase activity.
c) Preparation of Threonine
The invention further relates to a process for preparing threonine by culturing genetically modified microorganisms with increased or caused expression rate of at least one gene compared with the wild type, where
the expression activity in the microorganism of at least one endogenous gene is regulated by an expression unit of the invention, or the expression of at least one gene in the microorganism is caused or altered by introducing into said microorganism an expression unit of the invention comprising said gene.

The genes are selected here in particular from nucleic acids encoding an aspartate kinase, nucleic acids encoding an aspartate-semialdehyde dehydrogenase, nucleic acids encoding a glyceraldehyde-3-phosphate dehydrogenase, nucleic acids encoding a 3-phosphoglycerate kinase, nucleic acids encoding a pyruvate carboxylase, nucleic acids encoding a triosephosphate isomerase, nucleic acids encoding a homoserine kinase, nucleic acids encoding a threonine synthase, nucleic acids encoding a threonine exporter carrier, nucleic acids encoding a glucose-6-phosphate dehydrogenase, nucleic acids encoding a transaldolase, nucleic acids encoding a transketolase, nucleic acids encoding a malate-quinone oxidoreductase, nucleic acids encoding a 6-phosphogluconate dehydrogenase, nucleic acids encoding a lysine exporter, nucleic acids encoding a biotin ligase, nucleic acids encoding a phosphoenolpyruvate carboxylase, nucleic acids encoding a threonine efflux protein, nucleic acids encoding a fructose-1,6-bisphosphatase, nucleic acids encoding an OpcA protein, nucleic acids encoding a 1-phosphofructokinase, nucleic acids encoding a 6-phosphofructokinase, and nucleic acids encoding a homoserine dehydrogenase, and nucleic acids encoding a 6-phosphogluconate dehydrogenase, glucose phosphate isomerase, phosphoglycerate mutase, enolase, pyruvate kinase, aspartate transaminase and malate enzyme.
A further preferred embodiment of the process described above for preparing threonine comprises the genetically modified microorganisms having, compared with the wild type, additionally an increased activity of at least one of the activities selected from the group of:
aspartate kinase activity, aspartate-semialdehyde dehydrogenase activity, glyceraldehyde-3-phosphate dehydrogenase activity, 3-phosphoglycerate kinase activity, pyruvate carboxylase activity, triosephosphate isomerase activity, threonine synthase activity, activity of a threonine export carrier, transaldolase activity, transketolase activity, glucose-6-phosphate dehydrogenase activity, malate-quinone oxidoreductase activity, homoserine kinase activity, biotin ligase activity, phosphoenolpyruvate carboxylase activity, threonine efflux protein activity, protein OpcA activity, 1-phosphofructokinase activity, 6-phosphofructokinase activity, fructose-1,6-bisphosphatase activity, 6-phosphogluconate dehydrogenase, homoserine dehydrogenase activity and activity of a 6-phosphogluconate dehydrogenase, activity of a glucose phosphate isomerase, activity of a phosphoglycerate mutase, activity of an enolase, activity of a pyruvate kinase, activity of an aspartate transaminase and activity of the malate enzyme.
A further particularly preferred embodiment of the process described above for preparing threonine comprises the genetically modified microorganisms having, compared with the wild type, additionally a reduced activity of at least one of the activities selected from the group of threonine dehydratase activity, homoserine O-acetyltransferase activity, serine hydroxymethyltransferase activity, O-acetyl-homoserine sulfhydrylase activity, meso-diaminopimelate D-dehydrogenase activity, phosphoenolpyruvate carboxykinase activity, pyruvate oxidase activity, dihydrodipicolinate synthetase activity, dihydrodipicolinate reductase activity, asparaginase activity, aspartate decarboxylase activity, lysine exporter activity, acetolactate synthase activity, ketol-acid reductoisomerase activity, branched chain aminotransferase activity, coenzyme B12-dependent methionine synthase activity, coenzyme B12-independent methionine synthase activity, dihydroxy-acid dehydratase activity and diaminopicolinate decarboxylase activity.
d) Further Information on Preparing Bioproducts According to the Invention
These additional increased or reduced activities of at least one of the activities described above may, but need not, be caused by an expression unit of the invention or an expression cassette of the invention.
The term “activity” of a protein means in the case of enzymes the enzymic activity of the corresponding protein, and in the case of other proteins, for example structural or transport proteins, the physiological activity of the proteins.
The enzymes are ordinarily able to convert a substrate into a product or catalyze this conversion step. Accordingly, the “activity” of an enzyme means the quantity of substrate converted by the enzyme, or the quantity of product formed, in a particular time.
Thus, where an activity is increased compared with the wild type, the quantity of the substrate converted by the enzyme, or the quantity of product formed, in a particular time is increased compared with the wild type.
This increase in the “activity”, for example, amounts, for all activities described hereinbefore and hereinafter, to at least 1 to 5%, such as, for example, at least 20%, at least 50%, at least 100%, at least 300%, or at least 500%, or at least 600% of the “activity of the wild type”.
Thus, where an activity is reduced compared with the wild type, the quantity of substrate converted by the enzyme, or the quantity of product formed, in a particular time is reduced compared with the wild type.
A reduced activity preferably means the partial or substantially complete suppression or blocking, based on various cell biological mechanisms, of the functionality of this enzyme in a microorganism.
A reduction in the activity comprises a quantitative decrease in an enzyme as far as substantially complete absence of the enzyme (i.e. lack of detectability of the corresponding activity or lack of immunological detectability of the enzyme). The activity in the microorganism is preferably reduced, compared with the wild type, by at least 5%, such as by at least 20%, by at least 50%, or by about 100%. “Reduction” also means in particular the complete absence of the corresponding activity.
The activity of particular enzymes in genetically modified microorganisms and in the wild type, and thus the increase or reduction in the enzymic activity, can be measured by known methods such as, for example, enzyme assays.
An additional increasing of activities can take place in various ways, for example by switching off inhibitory regulatory mechanisms at the expression and protein level or by increasing gene expression of nucleic acids encoding the proteins described above compared with the wild type.
Increasing the gene expression of the nucleic acids encoding the proteins described above compared with the wild type can likewise take place in various ways, for example by inducing the gene by activators or, as described above, by increasing the promoter activity or increasing the expression activity or by introducing one or more gene copies into the microorganism.
Increasing the gene expression of a nucleic acid encoding a protein also means according to the invention manipulation of the expression of the endogenous proteins intrinsic to the microorganism. This can be achieved for example, as described above, by altering the promoter sequences of the genes, introducing an expression unit of the invention for regulatory control of the genes and altering the introduced expression units of the invention. Such an alteration, which results in an increased expression rate of the gene, can take place for example by deletion or insertion of DNA sequences.
It is possible, as described above, to alter the expression of the endogenous proteins by applying exogenous stimuli. This can take place through particular physiological conditions, i.e. through the application of foreign substances.
The skilled worker may have recourse to further different procedures, singly or in combination, to achieve an increase in gene expression. Thus, for example, the copy number of the appropriate genes can be increased, or the promoter and regulatory region or the ribosome binding site located upstream of the structural gene can be mutated. It is additionally possible to increase the expression during fermentative production through inducible promoters. Procedures to prolong the lifespan of the mRNA likewise improve expression. Enzymic activity is likewise enhanced also by preventing degradation of the enzyme protein. The genes or gene constructs may be either present in plasmids with varying copy number or integrated and amplified in the chromosome. It is also possible as an alternative to achieve overexpression of the relevant genes by altering the composition of the media and management of the culture.
The skilled worker can find guidance on this inter alia in Martin et al. (Biotechnology 5, 137-146 (1987)), in Guerrero et al. (Gene 138, 35-41 (1994)), Tsuchiya and Morinaga (Bio/Technology 6, 428-430 (1988)), in Eikmanns et al. (Gene 102, 93-98 (1991)), in EP-A 0472869, in U.S. Pat. No. 4,601,893, in Schwarzer and Pühler (Biotechnology 9, 84-87 (1991), in Reinscheid et al. (Applied and Environmental Microbiology 60, 126-132 (1994), in LaBarre et al. (Journal of Bacteriology 175, 1001-1007 (1993)), in WO 96/15246, in Malumbres et al. (Gene 134, 15-24 (1993)), in JP-A-10-229891, in Jensen and Hammer (Biotechnology and Bioengineering 58, 191-195 (1998)), in Makrides (Microbiological Reviews 60: 512-538 (1996) and in well-known textbooks of genetics and molecular biology.
It may additionally be advantageous for the production of biosynthetic products, especially L-lysine, L-methionine and L-threonine, besides the expression or enhancement of a gene, to eliminate unwanted side reactions (Nakayama: “Breeding of Amino Acid Producing Microorganisms”, in: Overproduction of Microbial Products, Krumphanzl, Sikyta, Vanek (eds.), Academic Press, London, UK, 1982).
In a preferred embodiment, gene expression of a nucleic acid encoding one of the proteins described above is increased by introducing at least one nucleic acid encoding a corresponding protein into the microorganism. The introduction of the nucleic acid can take place chromosomally or extrachromosomally, i.e. through increasing the copy number on the chromosome and/or a copy of the gene on a plasmid which replicates in the host microorganism.
The introduction of the nucleic acid, for example in the form of an expression cassette of the invention comprising the nucleic acid, preferably takes place chromosomally, in particular by the SacB method described above. It is possible in principle to use for this purpose any gene which encodes one of the proteins described above.
In the case of genomic nucleic acid sequences from eukaryotic sources which comprise introns, if the host microorganism is unable or cannot be made able to express the corresponding proteins it is preferred to use nucleic acid sequences which have already been processed, such as the corresponding cDNAs.
Examples of the corresponding genes are listed in Table 1 and 2.
The activities described above in microorganisms are preferably reduced by at least one of the following methods:
introduction of at least one sense ribonucleic acid sequence for inducing cosuppression or of an expression cassette ensuring expression thereof introduction of at least one DNA- or protein-binding factor against a corresponding gene, an RNA or a protein or of an expression cassette ensuring expression thereof introduction of at least one viral nucleic acid sequence which causes RNA degradation, or of an expression cassette ensuring expression thereof introduction of at least one construct to produce a loss of function, such as, for example, generation of stop codons or shifts in the reading frame, of a gene, for example by producing an insertion, deletion, inversion or mutation in a gene. It is possible and preferred to generate knockout mutants by targeted insertion into the desired target gene through homologous recombination or introduction of sequence-specific nucleases against the target gene. introduction of a promoter with reduced promoter activity or of an expression unit with reduced expression activity. introduction of an antisense RNA which reduces translation of the sense RNA (mRNA).

The skilled worker is aware that further processes can also be employed within the scope of the present invention for reducing its activity or function. For example, the introduction of a dominant negative variant of a protein or of an expression cassette ensuring expression thereof may also be advantageous.
It is moreover possible for each single one of these processes to bring about a reduction in the quantity of protein, quantity of mRNA and/or activity of a protein. A combined use is also conceivable. Further methods are known to the skilled worker and may comprise impeding or suppressing the processing of the protein, of the transport of the protein or its mRNA, inhibition of ribosome attachment, inhibition of RNA splicing, induction of an RNA-degrading enzyme and/or inhibition of translation elongation or termination.
VII. Cultivation of Microorganisms and Isolation of Products
In the process of the invention for preparing biosynthetic products, the step of culturing the genetically modified microorganisms is preferably followed by an isolation of biosynthetic products from the microorganisms and/or from the fermentation broth. These steps may take place at the same time and/or preferably after the culturing step.
The genetically modified microorganisms of the invention can be cultured to produce biosynthetic products, in particular L-lysine, L-methionine and L-threonine, continuously or discontinuously in a batch process (batch culturing) or in the fed batch or repeated fed batch process. A summary of known culturing methods is to be found in the textbook by Chemiel (Bioprozeβtechnik 1. Einführung in die Bioverfahrenstechnik (Gustav Fischer Verlag, Stuttgart, 1991)) or in the textbook by Storhas (Bioreaktoren und periphere Einrichtungen (Vieweg Verlag, Braunschweig/Wiesbaden, 1994)).
The culture medium to be used must satisfy in a suitable manner the demands of the respective strains. There are descriptions of culture media for various microorganisms in the handbook “Manual of Methods for General Bacteriology” of the American Society for Bacteriology (Washington D.C., USA, 1981).
These media which can be employed according to the invention usually comprise one or more carbon sources, nitrogen sources, inorganic salts, vitamins and/or trace elements.
Preferred carbon sources are sugars such as mono-, di- or polysaccharides. Examples of very good carbon sources are glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose. Sugars can be put in the media also via complex compounds such as molasses, or other by-products of sugar refining. It may also be advantageous to add mixtures of various carbon sources. Other possible carbon sources are oils and fats such as, for example, soybean oil, sunflower oil, peanut oil and coconut fat, fatty acids such as, for example, palmitic acid, stearic acid or linoleic acid, alcohols such as, for example, glycerol, methanol or ethanol and organic acids such as, for example, acetic acid or lactic acid.
Nitrogen sources are usually organic or inorganic nitrogen compounds or materials containing these compounds. Examples of nitrogen sources comprise ammonia gas or ammonium salts such as ammonium sulfate, ammonium chloride, ammonium phosphate, ammonium carbonate or ammonium nitrate, nitrates, urea, amino acids or complex nitrogen sources such as corn steep liquor, soybean flour, soybean protein, yeast extract, meat extract and others. The nitrogen sources may be used singly or as mixtures.
Inorganic salt compounds which may be present in the media comprise the chloride, phosphoric or sulfate salts of calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron.
For preparing fine chemicals, especially methionine, it is possible to use as sulfur source inorganic compounds such as, for example, sulfates, sulfites, dithionites, tetrathionates, thiosulfates, sulfides, but also organic sulfur compounds such as mercaptans and thiols.
It is possible to use as phosphorus source phosphoric acid, potassium dihydrogenphosphate or dipotassium hydrogenphosphate or the corresponding sodium-containing salts.
Chelating agents can be added to the medium in order to keep the metal ions in solution. Particularly suitable chelating agents comprise dihydroxyphenols such as catechol or protocatechuate, or organic acids such as citric acid.
The fermentation media employed according to the invention normally also comprise other growth factors such as vitamins or growth promoters, which include for example biotin, riboflavin, thiamine, folic acid, nicotinic acid, pantothenate and pyridoxine. Growth factors and salts are frequently derived from complex components of the media, such as yeast extract, molasses, corn steep liquor and the like. Suitable precursors may also be added to the culture medium. The exact composition of the compounds in the media depends greatly on the particular experiment and will be decided individually for each specific case. Information on optimization of media is obtainable from the textbook “Applied Microbiol. Physiology, A Practical Approach” (editors P. M. Rhodes, P. F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 3). Growth media can also be purchased from commercial suppliers, such as Standard 1 (Merck) or BHI (Brain heart infusion, DIFCO) and the like.
All the components of the media are sterilized either by heat (20 min at 1.5 bar and 121° C.) or by sterilizing filtration. The components can be sterilized either together or, if necessary, separately. All the components of the media may be present at the start of culturing or optionally be added continuously or batchwise.
The temperature of the culture is normally between 15° C. and 45° C., preferably at 25° C. to 40° C. and can be kept constant or changed during the experiment. The pH of the medium should be in the range from 5 to 8.5, preferably around 7.0. The pH for the culturing can be controlled during the culturing by adding basic compounds such as sodium hydroxide, potassium hydroxide, ammonia or aqueous ammonia or acidic compounds such as phosphoric acid or sulfuric acid. The development of foam can be controlled by employing antifoams such as, for example, fatty acid polyglycol esters. The stability of plasmids can be maintained by adding to the medium suitable substances with a selective action, such as, for example, antibiotics. Aerobic conditions are maintained by introducing oxygen or oxygen-containing gas mixtures such as, for example, ambient air into the culture. The temperature of the culture is normally 20° C. to 45° C. The culture is continued until formation of the desired product is at a maximum. This aim is normally reached within 10 hours to 160 hours.
The dry matter content of the fermentation broths obtained in this way is normally from 7.5 to 25% by weight.
It is additionally advantageous also to run the fermentation with sugar limitation at least at the end, but in particular over at least 30% of the fermentation time. This means that the concentration of utilizable sugar in the fermentation medium is kept at 0 to 3 g/l, or is reduced, during this time.
Biosynthetic products are isolated from the fermentation broth and/or the microorganisms in a manner known per se in accordance with the physical/chemical properties of the required biosynthetic product and the biosynthetic by-products.
The fermentation broth can then be processed further for example. Depending on the requirement, the biomass can be removed wholly or partly from the fermentation broth by separation methods such as, for example, centrifugation, filtration, decantation or a combination of these methods, or left completely in it.
The fermentation broth can then be thickened or concentrated by known methods such as, for example, with the aid of a rotary evaporator, thin-film evaporator, falling-film evaporator, by reverse osmosis or by nanofiltration. This concentrated fermentation broth can then be worked up by freeze drying, spray drying, spray granulation or by other processes.
However, it is also possible to purify the biosynthetic products, especially L-lysine, L-methionine and L-threonine, further. For this purpose, the product-containing broth is subjected, after removal of the biomass, to a chromatography using a suitable resin, with the desired product or the impurities being retained wholly or partly on the chromatography resin. These chromatographic steps can be repeated if required, using the same or different chromatography resins. The skilled worker is proficient in the selection of suitable chromatography resins and their most effective use. The purified product can be concentrated by filtration or ultrafiltration and be stored at a temperature at which the stability of the product is a maximum.
The biosynthetic products may result in various forms, for example in the form of their salts or esters.
The identity and purity of the isolated compound(s) can be determined by prior art techniques. These comprise high pressure liquid chromatography (HPLC), spectroscopic methods, staining methods, thin-layer chromatography, NIRS, enzyme assay or microbiological assays. These analytical methods are summarized in: Patek et al. (1994) Appl. Environ. Microbiol. 60:133-140; Malakhova et al. (1996) Biotekhnologiya 11 27-32; and Schmidt et al. (1998) Bioprocess Engineer. 19:67-70. Ullmann's Encyclopedia of Industrial Chemistry (1996) vol. A27, VCH: Weinheim, pp. 89-90, pp. 521-540, pp. 540-547, pp. 559-566, 575-581 and pp. 581-587; Michal, G (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.
The invention is now described in more detail by means of the following nonlimiting examples:
Example 1
Preparation of the Vector pCLiK5MCS
First, the ampicillin resistance and origin of replication of the vector pBR322 were amplified with the aid of the polymerase chain reaction (PCR) using the oligonucleotide primers SEQ ID NO:5 and SEQ ID NO: 6.





SEQ ID NO: 5:

5′-CCCGGGATCCGCTAGCGGCGCGCCGGCCGGCCCGGTGTGAAATACCG



CACAG-3′



SEQ ID NO: 6:

5′-TCTAGACTCGAGCGGCCGCGGCCGGCCTTTAAATTGAAGACGAAAGG



GCCTCG-3′
















Apart from the sequences complementary to pBR322, the SEQ ID NO: 5 oligonucleotide primer comprises, in the 5′-3′ direction, the cleavage sites for the restriction endonucleases SmaI, BamHI, NheI and AscI, and the SEQ ID NO: 6 oligonucleotide primer comprises, in the 5′-3′ direction, the cleavage sites for the restriction endonucleases XbaI, XhoI, NotI and DraI. The PCR reaction was carried out by a standard method such as Innis et al. (PCR Protocols. A Guide to Methods and Applications, Academic Press (1990)) using Pfu Turbo polymerase (Stratagene, La Jolla, USA). The DNA fragment obtained, whose size is approximately 2.1 kb, was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg, Germany) according to the manufacturer's information. The blunt ends of the DNA fragment were ligated to one another using the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer's information and the ligation mixture was transformed according to standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), in competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on ampicillin (50 μg/ml)-containing LB Agar (Lennox, 1955, Virology, 1:190).
The plasmid DNA of an individual clone was isolated using the Qiaprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer's information and checked via restriction digestions. The plasmid obtained in this way is denoted pCLiK1.
Starting from the plasmid pWLT1 (Liebl et al., 1992) as template for a PCR reaction, a kanamycin resistance cassette was amplified using the oligonucleotide primers SEQ ID NO:7 and SEQ ID NO:8.





SEQ ID NO: 7:

5′-GAGATCTAGACCCGGGGATCCGCTAGCGGGCTGCTAAAGGAAGCGG



A-3′



SEQ ID NO: 8

5′-GAGAGGCGCGCCGCTAGCGTGGGCGAAGAACTCCAGCA-3′














Apart from the sequences complementary to pWLT1, the SEQ ID NO: 7 oligonucleotide primer comprises, in the 5′-3′ direction, the cleavage sites for the restriction endonucleases XbaI, SmaI, BamHI, NheI, and the SEQ ID NO:8 oligonucleotide primer comprises, in the 5′-3′ direction, the cleavage sites for the restriction endonucleases AscI and NheI. The PCR reaction was carried out by standard method such as Innis et al. (PCR Protocols. A Guide to Methods and Applications, Academic Press (1990)), using Pfu Turbo polymerase (Stratagene, La Jolla, USA). The DNA fragment obtained, whose size is approximately 1.3 kb, was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. The DNA fragment was cut by the restriction endonucleases XbaI and AscI (New England Biolabs, Beverly, USA) and subsequently purified again using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. The pCLiK1 vector was likewise cut by the XbaI and AscI restriction endonucleases and dephosphorylated by alkali phosphatase (I (Roche Diagnostics, Mannheim)) according to the manufacturer's information. After electrophoresis in a 0.8% strength agarose gel, the linearized vector (approx. 2.1 kb) was isolated using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. This vector fragment was ligated with the cut PCR fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1 Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing ampicillin (50 μg/ml) and kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA of an individual clone was isolated using the Qiaprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer's information and checked via restriction digestions. The plasmid obtained in this way is denoted pCLiK2.
The pCLiK2 vector was cut by the restriction endonuclease DraI (New England Biolabs, Beverly, USA). After electrophoresis in a 0.8% strength agarose gel, an approx. 2.3 kb vector fragment was isolated with the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. This vector fragment was religated with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1 Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA of an individual clone was isolated using the Qiaprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer's information and checked via restriction digestions. The plasmid obtained in this way is denoted pCLiK3.
Starting from the plasmid pWLQ2 (Liebl et al., 1992) as template for a PCR reaction, the pHM1519 origin of replication was amplified using the oligonucleotide primers SEQ ID NO:9 and SEQ ID NO:10.







SEQ ID NO: 9:


5′-GAGAGGGCGGCCGCGCAAAGTCCCGCTTCGTGAA-3′




SEQ ID NO: 10:


5′-GAGAGGGCGGCCGCTCAAGTCGGTCAAGCCACGC-3′












Apart from the sequences complementary to pWLQ2, the SEQ ID NO:9 and SEQ ID NO:10 oligonucleotide primers comprise cleavage sites for the NotI restriction endonuclease. The PCR reaction was carried out by standard method such as Innis et al. (PCR Protocols. A Guide to Methods and Applications, Academic Press (1990)), using Pfu Turbo polymerase (Stratagene, La Jolla, USA). The DNA fragment obtained, whose size is approximately 2.7 kb, was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. The DNA fragment was cut by the restriction endonuclease NotI (New England Biolabs, Beverly, USA) and subsequently purified again using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. The pCLiK3 vector was likewise cut by the NotI restriction endonuclease and dephosphorylated by alkali phosphatase (I (Roche Diagnostics, Mannheim)) according to the manufacturer's information. After electrophoresis in a 0.8% strength agarose gel, the linearized vector (approx. 2.3 kb) was isolated using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. This vector fragment was ligated with the cut PCR fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA of an individual clone was isolated using the Qiaprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer's information and checked via restriction digestions. The plasmid obtained in this way is denoted pCLiK5.
In order to extend pCLiK5 by a “multiple cloning site” (MCS), the two synthetic, essentially complementary oligonucleotides SEQ ID NO:11 and SEQ ID NO:12, which comprise cleavage sites for the restriction endonucleases SwaI, XhoI, AatI, ApaI, Asp718, MluI, NdeI, SpeI, EcoRV, SalI, ClaI, BamHI, XbaI and SmaI were combined by heating them together to 95° C. followed by slow cooling to give a double-stranded DNA fragment.





SEQ ID NO: 11:

5′-TCGAATTTAAATCTCGAGAGGCCTGACGTCGGGCCCGGTACCACGCG



TCATATGACTAGTTCGGACCTAGGGATATCGTCGACATCGATGCTCTTCT



GCGTTAATTAACAATTGGGATCCTCTAGACCCGGGATTTAAAT-3′



SEQ ID NO: 12:

5′-GATCATTTAAATCCCGGGTCTAGAGGATCCCAATTGTTAATTAACGC



AGAAGAGCATCGATGTCGACGATATCCCTAGGTCCGAACTAGTCATATGA



CGCGTGGTACCGGGCCCGACGTCAGGCCTCTCGAGATTTAAAT-3′




















The pCLiK5 vector was cut by the restriction endonucleases XhoI and BamHI (New England Biolabs, Beverly, USA) and dephosphorylated by alkali phosphatase (I (Roche Diagnostics, Mannheim)) according to the manufacturer's information. After electrophoresis in a 0.8% strength agarose gel, the linearized vector (approx. 5.0 kb) was isolated using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. This vector fragment was ligated with the synthetic double-stranded DNA fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1 Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA of an individual clone was isolated using the Qiaprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer's information and checked via restriction digestions. The plasmid obtained in this way is denoted pCLiK5MCS.
Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt, Germany).
The resultant plasmid, pCLiK5MCS, is listed as SEQ ID NO:13.
Example 2
Preparation of the Plasmid PmetA metA
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 14 and SEQ ID NO: 15, the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), the metA gene, including the noncoding 5′ region, was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.





SEQ ID NO: 14





5′-GCGCGGTACCTAGACTCACCCCAGTGCT-3′


and





SEQ ID NO: 15





5′-CTCTACTAGTTTAGATGTAGAACTCGATGT-3′






















The approx. 1.3 kb DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes Asp718 and SpeI (Roche Diagnostics, Mannheim), and the DNA fragment was purified using the GFX™ PCR, DNA and Gel Band Purification Kit.
The vector pClik5MCS SEQ ID NO: 13 was cut by the Asp718 and SpeI restriction enzymes, and a 5 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The vector fragment was ligated together with the PCR fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resultant plasmid, pCLiK5MCS PmetA metA, is listed as SEQ ID NO 16.
Example 3
Preparation of the Plasmid pCLiK5MCS Psod metA
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 17 and SEQ ID NO: 18, the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 200 base pairs from the noncoding 5′ region (region of the expression unit) of superoxide dismutase (Psod) was amplified with the aid of the polymerase chain reaction (PCR) by standard methods such as Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.





SEQ ID NO: 17

5′-GAGACTCGAGAGCTGCCAATTATTCCGGG-3′

and



SEQ ID NO: 18

5′-CCTGAAGGCGCGAGGGTGGGCATGGGTAAAAAATCCTTTCG-3′













The DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
Starting from the PmetA metA SEQ ID NO: 16 plasmid as template for a PCR reaction, metA was partially amplified using the oligonucleotide primers SEQ ID NO: 19 and SEQ ID NO: 20.





SEQ ID NO: 19





5′-CCCACCCTCGCGCCTTCAG-3′


and





SEQ ID NO: 20





5′-CTGGGTACATTGCGGCCC-3′






















The DNA fragment obtained of approximately 470 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information.
The two fragments obtained above were used together as template in a further PCR reaction. In the course of the PCR reaction, the metA-homologous sequences introduced with the oligonucleotide primer SEQ ID NO: 18 cause the two fragments to attach to one another and to be extended by the polymerase used to give a continuous DNA strand. The standard method was modified in that the oligonucleotide primers used, SEQ ID NO: 17 and SEQ ID NO: 20, were only added to the reaction mixture at the start of the 2nd cycle.
The amplified DNA fragment of approximately 675 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes XhoI and NcoI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 620 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The PmetA metA SEQ ID NO: 16 plasmid was cleaved by the restriction enzymes NcoI and SpeI (Roche Diagnostics, Mannheim). After fractionation by gel electrophoresis, an approx. 0.7 kb metA fragment was purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The pClik5MCS SEQ ID NO: 13 vector was cut by the restriction enzymes XhoI and SpeI (Roche Diagnostics, Mannheim), and a 5 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The vector fragment was ligated together with the PCR fragment and the metA fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The plasmid obtained, pCLiK5MCS PSODmetA, is listed as SEQ ID NO: 21.
Example 4
Preparation of the Plasmid pCLiK5MCS P EF-TU metA
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 22 and SEQ ID NO: 23, the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 200 base pairs from the noncoding 5′ region (promoter region) of superoxide dismutase (Psod) was amplified with the aid of the polymerase chain reaction (PCR) by standard methods such as Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.





SEQ ID NO: 22

5′-GAGACTCGAGGGCCGTTACCCTGCGAATG-3′

and



SEQ ID NO: 23

5′-CCTGAAGGCGCGAGGGTGGGCATTGTATGTCCTCCTGGAC-3′













The DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
Starting from the PmetA metA SEQ ID NO: 16 plasmid as template for a PCR reaction, metA was partially amplified using the oligonucleotide primers SEQ ID NO: 24 and SEQ ID NO: 25.





SEQ ID NO: 24





5′-CCCACCCTCGCGCCTTCAG-3′


and





SEQ ID NO: 25





5′-CTGGGTACATTGCGGCCC-3′






















The DNA fragment obtained of approximately 470 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information.
The two fragments obtained above were used together as template in a further PCR reaction. In the course of the PCR reaction, the metA-homologous sequences introduced with the oligonucleotide primer SEQ ID NO: 18 cause the two fragments to attach to one another and to be extended by the polymerase used to give a continuous DNA strand. The standard method was modified in that the oligonucleotide primers used, SEQ ID NO: 22 and SEQ ID NO: 25, were only added to the reaction mixture at the start of the 2nd cycle.
The amplified DNA fragment of approximately 675 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes XhoI and NcoI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 620 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The PmetA metA SEQ ID NO: 16 plasmid was cleaved with the restriction enzymes NcoI and SpeI (Roche Diagnostics, Mannheim). After fractionation by gel electrophoresis, an approx. 0.7 kb metA fragment was purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The pClik5MCS SEQ ID NO: 13 vector was cut by the restriction enzymes XhoI and SpeI (Roche Diagnostics, Mannheim), and a 5 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The vector fragment was ligated together with the PCR fragment and the metA fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The plasmid obtained, pCLiK5MCS P_EFTUmetA, is listed as SEQ ID NO: 26.
Example 5
Preparation of the Plasmid pCLiK5MCS Pgro metA
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 27 and SEQ ID NO: 28, the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 200 base pairs from the noncoding 5′ region (promoter region) of GroES gene (Pgro) was amplified with the aid of the polymerase chain reaction (PCR) by standard methods such as Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.





SEQ ID NO: 27

5′-GAGACTCGAGCGGCTTAAAGTTTGGCTGCC-3′



SEQ ID NO: 28

5′-CCTGAAGGCGCGAGGGTGGGCATGATGAATCCCTCCATGAG-3′












The DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
Starting from the PmetA metA plasmid as template for a PCR reaction, metA was partially amplified using the oligonucleotide primers SEQ ID NO: 29: and SEQ ID NO: 30.





SEQ ID NO: 29





5′-CCCACCCTCGCGCCTTCAG-3′





SEQ ID NO: 30





5′-CTGGGTACATTGCGGCCC-3′





















The DNA fragment obtained of approximately 470 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information.
The two fragments obtained above were used together as template in a further PCR reaction. In the course of the PCR reaction, the metA-homologous sequences introduced with the oligonucleotide primer SEQ ID NO: 28 cause the two fragments to attach to one another and to be extended by the polymerase used to give a continuous DNA strand. The standard method was modified in that the oligonucleotide primers used, SEQ ID NO: 27 and SEQ ID NO: 30, were only added to the reaction mixture at the start of the 2nd cycle.
The amplified DNA fragment of approximately 675 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes XhoI and NcoI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 620 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The PmetA metA SEQ ID NO: 16 plasmid was cleaved by the restriction enzymes NcoI and SpeI (Roche Diagnostics, Mannheim). After fractionation by gel electrophoresis, an approx. 0.7 kb metA fragment was purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The pClik5MCS SEQ ID NO: 13 vector was cut by the restriction enzymes XhoI and SpeI (Roche Diagnostics, Mannheim), and a 5 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The vector fragment was ligated together with the PCR fragment and the metA fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The plasmid obtained, pCLiK5MCS Pgro metA, is listed as SEQ ID NO: 31.
Example 6
Preparation of the Plasmid P EF-TS metA
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers BK 1849 (SEQ ID NO: 32) and BK 1862 (SEQ ID NO: 33), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), the MetaA gene which codes for homoserine O-acetyltransferase was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







BK 1849



(SEQ ID NO: 32)





5′-GTGTGTCGACTTAGATGTAGAACTCGATGTAG-3′


and




BK 1862



(SEQ ID NO: 33)





5′-ATGCCCACCCTCGCGCC-3′



























The approx. 1134 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
Using the oligonucleotide primers Haf 26 (SEQ ID NO: 34) and Haf 27 (SEQ ID NO: 35), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 200 base pairs from the noncoding 5′ region (region of the expression unit) of the gene coding for elongation factor TS was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.





Haf 26

(SEQ ID NO: 34)

5′-GAGAGGATCCCCCCCACGACAATGGAAC-3′

and



Haf 27

(SEQ ID NO: 35)

5′-CCTGAAGGCGCGAGGGTGGGCATTACGGGGCGATCCTCCTTATG-3′















The approx. 195 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
The Haf 27 and BK 1862 primers comprise an overlapping sequence and their 5′ ends are homologous to one another.
The PCR products obtained above were used as template for another PCR which made use of the primers BK 1849 (SEQ ID NO: 32) and Haf 26 (SEQ ID NO: 34).
Using this approach, a DNA fragment was amplified which corresponded to the expected size of 1329 bp. This P EF-TS/metA fusion was then cloned via the BamHI and SalI restriction cleavage sites into pClik 5a MCS (SEQ ID NO: 13) vector.
The vector fragment was ligated together with the PCR fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resulting plasmid was referred to as pClik 5a MCS P EF-TS metA (SEQ ID NO: 36).
Example 7
Preparation of the Plasmid P EF-Tu pSOD metA (H473)
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers BK 1753 (SEQ ID NO: 37) and BK 1754 (SEQ ID NO: 38), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), the GroEL terminator was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







BK 1753



(SEQ ID NO: 37)





GGATCTAGAGTTCTGTGAAAAACACCGTG




BK 1754



(SEQ ID NO: 38)





GCGACTAGTGCCCCACAAATAAAAAACAC


























The approx. 77 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information, cut by the Xba I and Bcu I restriction enzymes and purified once more.
The pClik5MCS (SEQ ID NO: 13) vector was linearized by the XbaI restriction enzyme and purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
The linearized vector was ligated together with the PCR fragment with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resulting plasmid was referred to as H247 (SEQ ID NO: 39).
This plasmid was treated with the BcuI and SalI restriction enzymes and purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
A PCR using the oligonucleotides BK 1848 (SEQ ID NO: 40) and BK 1849 (SEQ ID NO: 41), the pClik5MCS Psod metA (SEQ ID NO: 21) plasmid as template and Pfu Turbo polymerase (Stratagene) was carried out by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. The amplified fragment had an expected length of approx. 1350 bp and was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information, cut by the BcuI and SalI restriction enzymes and purified once more.
This fragment was ligated with the H247 plasmid (BcuI and SalI cleavage sites) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, beschrieben (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resulting plasmid was referred to as pG A4 (H344) and is listed under SEQ ID NO: 42.





SEQ ID NO: 40





(BK 1848) GAGAACTAGTAGCTGCCAATTATTCCGGG





SEQ ID NO: 41





(BK 1849) GTGTGTCGACTTAGATGTAGAACTCGATGTAG





















The pG A4 (SEQ ID NO: 42) plasmid was cut by the XhoI and BcuI restriction enzymes and purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
Construction of H473:
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotides primers BK 1695 (SEQ ID NO: 43) and Haf16 (SEQ ID NO: 44), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 182 base pairs from the noncoding 5′ region (region of the expression unit) of the EF Tu gene (Peftu) was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







SEQ ID NO: 43


(BK1695) GAGACTCGAGGGCCGTTACCCTGCGAATG





SEQ ID NO: 44


(Haf16) GAGAACTAGTGTGGCTACGACTTTCGCAGC












The approx. 200 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information, cut by the Xho I and Bcu I restriction enzymes and purified once more.
This fragment was ligated with the pG A4 (H344) plasmid (XhoI and BcuI cleavage sites) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid obtained was referred to as pClik5MCS PeftuPsod metA (H473). It is listed under SEQ ID NO: 45. It comprises (from 5′ to 3′) a Peftu promoter, a Psod expression unit and, immediately downstream thereof, the metA open reading frame.
Example 8
Preparation of the plasmid PsodPeftu metA (H505)
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 46 (Haf64) and SEQ ID NO: 47 (Haf65), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 200 base pairs from the noncoding 5′ region (region of the expression unit) of the EF Tu gene (Peftu) was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







SEQ ID NO: 46


(Haf64) GAGAACTAGTGGCCGTTACCCTGCGAATG





SEQ ID NO: 47


(Haf65) GCGCGAGGGTGGGCATTGTATGTCCTCCTGGACTTC












The approx. 200 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
In a second PCR, using the oligonucleotide primers BK1862 (SEQ ID NO: 48) and BK1849 (SEQ ID NO: 49) and the H344 (SEQ ID NO: 42) plasmid as template, the metA open reading frame was amplified by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. The approx. 1140 bp fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.







SEQ ID NO: 48


(BK1862) ATGCCCACCCTCGCGCC





SEQ ID NO: 49


(BK1849) GTGTGTCGACTTAGATGTAGAACTCGATGTAG












The two fragments obtained above were used together as template in a further PCR reaction. In the course of the PCR reaction, the metA-homologous sequences introduced with the oligonucleotide primer Haf 65 SEQ ID NO: 47 cause the two fragments to attach to one another and to be extended by the polymerase used to give a continuous DNA strand. The standard method was modified in that the oligonucleotide primers used, Haf 64 and BK 1849 SEQ ID NO: 46 and SEQ ID NO: 49 were only added to the reaction mixture at the start of the 2nd cycle.
The amplified DNA fragment of approximately 1350 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes BcuI and SalI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 1340 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg). This fragment comprises the Peftu expression unit fused to the metA ORF (=Peftu-metA fragment).
Using the oligonucleotide primers BK 1697 (SEQ ID NO: 50) and Haf17 (SEQ ID NO: 51), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment (total length: 193 bp, 173 thereof being pSOD) from the noncoding 5′ region (region of the expression unit) of the SOD gene (Psod) amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







SEQ ID NO: 50


(BK1697) GAGACTCGAGAGCTGCCAATTATTCCGGG





SEQ ID NO: 51


(Haf17) GAGAACTAGTTAGGTTTCCGCACCGAGC












The amplified DNA fragment was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes XhoI and BcuI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 180 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) (=Psod fragment).
The (H344) pG A4 SEQ ID NO: 42 plasmid was cleaved by the restriction enzymes XhoI and SalI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The linearized vector was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The linearized vector was ligated with the two fragments (Peftu-metA fragment and Psod fragment) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resultant plasmid, pClik5MCS PsodPeftu metA, is listed as SEQ ID NO: 52.
Example 9
Preparation of the Plasmid pClik5MCS Pgro Psod metA (H472)
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 53 (BK1701) and SEQ ID NO: 54 (Haf18), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), an approx. 175 nucleotide DNA fragment was and amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. It comprises 155 base pairs from the noncoding 5′ region (region of the expression unit) of the GRO EL gene (Pgro) and one restriction cleavage site each on the 5′ and 3′ ends (XhoI and BcuI, respectively).







SEQ ID NO: 53


(BK1701) GAGACTCGAGCGGCTTAAAGTTTGGCTGCC





SEQ ID NO: 54


(Haf018) GAGAACTAGTATTTTGTGTGTGCCGGTTGTG












The approx. 175 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes XhoI and BcuI (Roche Diagnostics, Mannheim), and the DNA fragment was purified using the GFX™ PCR, DNA and Gel Band Purification Kit.
The H344 (SEQ ID NO: 42) plasmid was cut by the XhoI and BcuI restriction enzymes, and an approx. 6.4 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
The PCR product and the cut-open H344 plasmid were ligated with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1 Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467.
The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resulting plasmid comprises a Pgro promoter immediately followed by a Psod expression unit and the metA ORF.
It is listed as pCLiK5MCS PgroPsod metA (H472) under SEQ ID NO: 55.
Example 10
Preparation of the Plasmid PgroPsod Pefts metA (H501)
A PCR using the oligonucleotide primers BK1782 (SEQ ID NO: 56) and Haf63 (SEQ ID NO: 57) and the pClik5MCS Pgro Psod metA (SEQ ID NO: 55 (H472)) plasmid as template was carried out by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. The amplified fragment comprises approx. 474 base pairs and a Pgro-Psod cassette with an Acc65I cleavage site at the 3′ end. The DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. It was subsequently cleaved by the XhoI and Acc65I restriction enzymes, and the DNA fragment (333 base pairs) was purified using the GFX™ PCR, DNA and Gel Band Purification Kit.







SEQ ID NO: 56


(BK1782) TCGAGAGATTGGATTCTTAC





SEQ ID NO: 57


(Haf 63) TCTCGGTACCCCGCACCGAGCATATACATC












The H479 (SEQ ID NO: 58) plasmid comprises the Pefts expression unit fused to the metA ORF. It was cut (directly upstream of Pefts) by the XhoI and Acc65I restriction enzymes, and an approx. 6.4 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
It was then ligated with the PCR fragment (Pgro-Psod cassette) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resulting plasmid comprises the 3 promoters, Pgro, Psod and P EF-Ts, fused to the metA ORF.
It is listed as pCLiK5MCS Pgro Psod Pefts metA (H501) under SEQ ID NO: 59.
Example 11
Preparation of the Plasmid Peftu Psod Pefts metA (H502)
A PCR using the oligonucleotide primers BK1782 (SEQ ID NO: 56) and Haf63 (SEQ ID NO: 57) and the pClik5MCS Peftu Psod (SEQ ID NO: 45 (H473)) plasmid as template was carried out by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. The amplified fragment comprises approx. 495 base pairs and a Peftu-Psod cassette with an Acc65I cleavage site at the 3′ end. The DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. It was subsequently cleaved by the XhoI and Acc65I restriction enzymes, and the DNA fragment (354 base pairs) was purified using the GFX™ PCR, DNA and Gel Band Purification Kit.







SEQ ID NO: 56


(BK1782)TCGAGAGATTGGATTCTTAC





SEQ ID NO: 57


(Haf 63) TCTCGGTACCCCGCACCGAGCATATACATC












The H479 (SEQ ID NO: 58) plasmid comprises the Pefts expression unit fused to the metA ORF. It was cut (directly upstream of Pefts) by the XhoI and Acc65I restriction enzymes, and an approx. 6.4 kb fragment was isolated, after electrophoretic fractionation, using the GFX™ PCR, DNA and Gel Band Purification Kit.
It was then ligated with the PCR fragment (Peftu-Psod cassette) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resulting plasmid comprises the 3 regulatory sequences, Peftu, Psod and P EF-Ts, fused to the metA ORF. It is listed as pCLiK5MCS Peftu Psod Pefts metA (H501) under SEQ ID NO: 60.
Example 12
Measurement of metA Activities
The Corynebacterium glutamicum strain ATCC13032 was transformed in each case with the plasmids pClik5 MCS, pClik MCS Psod metA, pClik MCS Peftu metA, pClik5 MCS Pefts metA, pClik5 MCS Peftu Psod metA, pClik5 MCS Psod Peftu metA, pClik5 MCS Pgro Psod meta, pClik5 MCS Pgro Psod Pefts metA, pClik5 MCS Peftu Psod Pefts metA according to the method described (Liebl, et al. (1989) FEMS Microbiology Letters 53:299-303). The transformation mixture was plated on CM plates which additionally contained 20 mg/l kanamycin in order to achieve selection for plasmid-containing cells. Kan-resistant clones obtained were picked and thinned out.
C. glutamicum strains comprising any of said plasmid constructs were grown in MMA Medium ((40 g/l sucrose, 20 g/l (NH 4 ) 2 SO 4 , 1 g/l KH 2 PO 4 , 1 g/l K 2 HPO 4 , 0.25 g/MgSO 4 ×7H 2 O, 54 g of Aces, 1 ml of CaCl 2 (10 g/l), 1 ml of protocatechuate (300 mg/10 ml), 1 ml of trace element solution (10 g/l FeSO 4 x/H 2 O, 10 g/l MnSO 4 ×H 2 O, 2 g/l ZnSO 4 ×7H 2 O, 0.2 g/l CuSO 4 , 0.02 g/l NiCl 2 ×6H 2 O), 100 μg/l vitamin B 12 , 0.3 mg/l thiamine, 1 mM leucine, 1 mg/l pyridoxal HCl, 1 ml of biotin (100 mg/l), pH 7.0) at 30° C. overnight. The cells were removed by centrifugation at 4° C. and washed twice with cold Tris-HCl buffer (0.1%, pH 8.0). After another centrifugation, the cells were taken up in cold Tris-HCl buffer (0.1%, pH 8.0) and the OD 600 was adjusted to 160. The cells were disrupted by transferring 1 ml of this cell suspension to 2-ml Hybaid Ribolyser tubes and lysed three times for in each case 30 s in a Hybaid Ribolyser, with rotation set to 6.0. The lysate was clarified by centrifugation in an Eppendorf centrifuge at 15 000 rpm and 4° C. for 30 minutes, and the supernatant was transferred to a new Eppendorf cup. The protein content was determined according to Bradford, M. M. (1976) Anal. Biochem. 72:248-254.
The enzymatic activity of MetA was carried out as follows. The 1-ml reaction mixtures contained 100 mM potassium phosphate buffer (pH 7.5), 5 mM MgCl 2 , 100 μM acetyl-CoA, 5 mM L-homoserine, 500 μM DTNB (Ellman's Reagent) and cell extract. The assay was started by adding the relevant protein lysate and incubated at room temperature. Kinetics were then recorded at 412 nm for 10 min.
The results are depicted in Table 1a.







TABLE 1a




Internal



construct
Specific

Strain
name
activity









ATCC 13032 pClik5MCS
H356
3.1


(metZ)


ATCC 13032 pCLiK5MCS Psod metA
H144
1308.7

ATCC 13032 pCLiK5MCS Peftu metA
H146
1233.0

ATCC 13032 pCLiK5MCS Pefts metA
H479
2339.0

ATCC 13032 pCLiK5MCS Peftu Psod metA
H473
2308.1

ATCC 13032 pCLiK5MCS Psod Peftu metA
H505
2061.9

ATCC 13032 pCLiK5MCS Pgro Psod metA
H472
1501.6

ATCC 13032 pCLiK5MCS Pgro Psod Pefts metA
H501
1773.4

ATCC 13032 pCLiK5MCS Peftu Psod Pefts metA
H502
2262.2
































It was possible to modulate the MetA activity by using the various combinations of promoters/expression units.
Example 13
Construction of the Plasmid pCIS lysC
In order to generate a lysine-producing strain, an allelic substitution of the lysC wild-type gene was carried out in Corynebacterium glutamicum ATCC13032. This involved a nucleotide substitution in the lysC gene so that the amino acid Thr at position 311 was replaced by an Ile in the resulting protein. Starting from the chromosomal DNA of ATCC13032 as template for a PCR reaction, lysC was amplified with the aid of the Pfu-Turbo PCR Systems (Stratagene USA) using the oligonucleotide primers SEQ ID NO: 61 and SEQ ID NO: 62, according to the manufacturer's information.





SEQ ID NO: 61





5′-GAGAGAGAGACGCGTCCCAGTGGCTGAGACGCATC-3′





SEQ ID NO: 62





5′-CTCTCTCTGTCGACGAATTCAATCTTACGGCCTG-3′





















C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. The amplified fragment is flanked by a SalI restriction cut on its 5′ end and by a MluI restriction cut on its 3′ end. Prior to cloning, the amplified fragment was digested by these two restriction enzymes and purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The polynucleotide obtained was cloned via the SalI and MluI restriction cuts into pCLIK5 MCS integrative SacB, referred to as pCIS hereinbelow (SEQ ID NO: 63), and transformed into E. coli XL-1 blue. Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190). The plasmid was isolated and the expected nucleotide sequence was confirmed by sequencing. The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt). The plasmid obtained, pCIS lysC, is listed as SEQ ID NO: 64.
Example 14
Mutagenesis of the C. glutamicum lysC Gene
Directed mutagenesis of the C. glutamicum lysC gene was carried out using the Quick-Change Kit (Stratagene/USA) according to the manufacturer's information. The mutagenesis was carried out in the pCIS lysC, SEQ ID NO:64, plasmid. To replace thr 311 with 311ile with the aid of the Quick-Change method (Stratagene), the following oligonucleotide primers were synthesized:





SEQ ID NO: 65





5′-CGGCACCACCGACATCATCTTCACCTGCCCTCGTTCCG-3′





SEQ ID NO: 66





5′-CGGAACGAGGGCAGGTGAAGATGATGTCGGTGGTGCCG-3′





















The use of these oligonucleotide primers in the Quick-Change reaction results in a substitution of the nucleotide in position 932 (from C to T) in the lysC gene SEQ ID NO: 67. The resulting amino acid substitution, Thr311Ile, in the lysC gene was confirmed by a sequencing reaction, after transformation into E. coli XL1-blue and plasmid preparation. The plasmid was denoted pCIS lysC thr311 ile and is listed as SEQ ID NO: 68.
The pCIS lysC thr311ile plasmid was transformed into C. glutamicum ATCC13032 by means of electroporation as described in Liebl, et al. (1989) FEMS Microbiology Letters 53:299-303. Modifications of the protocol are described in DE 10046870. The chromosomal arrangement of the lysC locus of individual transformants was checked by standard methods through Southern blot and hybridization, as described in Sambrook et al. (1989), Molecular Cloning. A Laboratory Manual, Cold Spring Harbor. This ensured that the transformants have integrated the transformed plasmid at the lysC locus by way of homologous recombination. After growing such colonies in media which do not contain any antibiotic overnight, the cells are plated out on a sucrose CM-agar medium (10% sucrose, 10 g/l glucose; 2.5 g/l NaCl; 2 g/l urea, 10 g/l Bacto Peptone (Difco); 10 g/l yeast extract, 22.0 g/L Agar (Difco)) and incubated at 30° C. for 24 hours.
Since the sacB gene present in the pCIS lysC thr311ile vector converts sucrose into a toxic product, only those colonies can grow in which the sacB gene has been deleted by a second homologous recombination step between the wild-type lysC gene and the mutated lysC thr311ile gene. It is possible, during homologous recombination, for either the wild-type gene or the mutated gene to be deleted together with the sacB gene. If the sacB gene is removed together with the wild-type gene, the result is a mutated transformant.
Growing colonies are picked and tested for a kanamycin-sensitive phenotype. Clones with deleted sacB gene must, at the same time, display kanamycin-sensitive growth behavior. Such Kan-sensitive clones were tested in a shaker flask for their lysine productivity (see Example 19). The untreated C. glutamicum ATCC13032 was grown for comparison. Clones having increased lysine production, compared with the control, were selected, chromosomal DNA was recovered and the corresponding region of the lysC gene was amplified by a PCR reaction and sequenced. A clone of this kind, which has the property of increased lysine synthesis and a detected mutation in lysC at position 932, was denoted ATCC13032 lysC fbr .
Example 15
Preparation of the Plasmid pCIS Peftu ddh
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828.
Using the oligonucleotide primers SEQ ID NO: 69 (Old38) and SEQ ID NO: 70 (Old 39), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 200 base pairs from the noncoding 5′ region (region of the expression unit) of the EFTu gene (Peftu) was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







SEQ ID NO: 69


(Old38) ACATCCATGGTGGCCGTTACCCTGCGAAT





SEQ ID NO: 70


(Old39) TGTATGTCCTCCTGGACTTC












The approx. 200 bp DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.
In a second PCR, using the oligonucleotide primers Old40 (SEQ ID NO: 71) and SEQ ID NO: 72 (Old 37) and C. glutamicum ATCC 13032 chromosomal DNA as template, the ddh open reading frame was amplified by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. The approx. 1017 bp fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information.







(SEQ ID NO: 71)


Old40 GAAGTCCAGGAGGACATACAATGACCAACATCCGCGTAGC





(SEQ ID NO: 72)


Old37 GAAACCACACTGTTTCCTTGC












The two fragments obtained above were used together as template in a further PCR reaction. In the course of the PCR reaction, the Peftu-homologous sequences introduced with the oligonucleotide primer Old40 SEQ ID NO: 71 cause the two fragments to attach to one another and to be extended by the polymerase used to give a continuous DNA strand. The standard method was modified in that the oligonucleotide primers used, Old37 and Old 38 (SEQ ID NO: 72 and SEQ ID NO: 69), were only added to the reaction mixture at the start of the 2nd cycle.
The amplified DNA fragment of approximately 1207 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes NcoI and XholI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 1174 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg). This fragment comprises the Peftu expression unit fused to the ddh ORF (=Peftu-ddh fragment).
Using the oligonucleotide primers Old 32 (SEQ ID NO: 73) and Old 33 (SEQ ID NO: 74), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment from the noncoding 5′ region of the ddh gene was amplified with the aid of the polymerase chain reaction (PCR) by standard methods as described in Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press.







SEQ ID 73


(Old32) ATCAACGCGTCGACACCACATCATCAATCAC





SEQ ID 74


(Old33) ATCACCATGGGTTCTTGTAATCCTCCAAAATTG












The amplified DNA fragment was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes MluI and NcoI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 720 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) (=5′ ddh fragment).
The pCIS (SEQ ID NO: 63) plasmid was cleaved by the restriction enzymes MluI and XhoI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The linearized vector was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The linearized vector was ligated with the two fragments (Peftu-ddh fragment and 5′ ddh fragment) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA was prepared by methods and with materials from Quiagen. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt).
The resultant plasmid, pCIS Peftu ddh, is listed as SEQ ID NO: 75.
Example 16
Preparation of pCIS PeftuPsod Ddh
C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 76 and SEQ ID NO: 77, the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 677 base pairs from the 5′ region of the ddh gene was amplified with the aid of the polymerase chain reaction (PCR) by standard methods such as Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press (5′ ddh fragment).







SEQ ID NO: 76


(CK461) CCTGACGTCGCAATATAGGCAGCTGAATC





SEQ ID NO: 77


(CK466) GCCCAATTGGTTCTTGTAATCCTCCAAAA












The DNA fragment obtained was purified using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. It was subsequently cleaved by the restriction enzymes AatlI and MunI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 661 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg). The resultant fragment comprises the 5′ region of the ddh ORF.
Starting from the P EF-Tu pSOD metA (H473) (SEQ ID 45) plasmid as template for a PCR reaction, a PeftuPsod cassette was amplified using the oligonucleotide primers SEQ ID NO: 78 and SEQ ID NO: 79.





SEQ ID NO: 78

(CK426) CGCCAATTGTCGAGGGCCGTTACCCT



SEQ ID NO: 79

(CK463) GCTACGCGGATGTTGGTCATGGGTAAAAAATCCTTTCGTA












The DNA fragment obtained of approximately 378 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information.
The ddh ORF was amplified in a subsequent PCR. For this purpose, C. glutamicum ATCC 13032 chromosomal DNA was prepared according to Tauch et al. (1995) Plasmid 33:168-179 or Eikmanns et al. (1994) Microbiology 140:1817-1828. Using the oligonucleotide primers SEQ ID NO: 80 (CK464) and SEQ ID NO: 81 (CK467), the chromosomal DNA as template and Pfu Turbo polymerase (Stratagene), a DNA fragment of approx. 992 base pairs (ddh ORF) was amplified with the aid of the polymerase chain reaction (PCR) according to standard methods such as Innis et al. (1990) PCR Protocols. A Guide to Methods and Applications, Academic Press. The amplified DNA fragment was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information.





SEQ ID NO: 80

(CK464) TACGAAAGGATTTTTTACCCATGACCAACATCCGCGTAGC



SEQ ID NO: 81

(CK467) AGACCCGGGTTAGACGTCGCGTGCGATCA












The two fragments obtained above (PeftuPsod cassette and ddh ORF) were used together as template in a further PCR reaction. In the course of the PCR reaction, the Psod-homologous sequences introduced with the oligonucleotide primer SEQ ID NO: 80 (CK464) cause the two fragments to attach to one another and to be extended by the polymerase used to give a continuous DNA strand. The standard method was modified in that the oligonucleotide primers used, SEQ ID NO: 79 (CK426) and SEQ ID NO: 81 (CK467), were only added to the reaction mixture at the start of the 2nd cycle.
The amplified DNA fragment of approximately 1359 base pairs was purified using the GFX™ PCR, DNA and Gel Band Purification Kit according to the manufacturer's information.
It was subsequently cleaved by the restriction enzymes MunI and SmaI (Roche Diagnostics, Mannheim) and fractionated by gel electrophoresis. The approx. 1359 base pair DNA fragment was then purified from the agarose, using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg).
The resultant fragment comprises (from 5′ to 3′) a Peftu promoter, a Psod expression unit and, immediately downstream thereof, the ddh open reading frame (PeftuPsod ddh).
The pCIS vector was cut by the AatlI and SmaI restriction endonucleases and dephosphorylated by alkali phosphatase (Roche Diagnostics, Mannheim) according to the manufacturer's information. After electrophoresis in a 0.8% strength agarose gel, the linearized vector (approx. 4.2 kb) was isolated using the GFX™ PCR, DNA and Gel Band Purification Kit (Amersham Pharmacia, Freiburg) according to the manufacturer's information. This vector fragment was ligated with the two cut PCR fragments (5′ddh and PeftuPsod ddh) with the aid of the Rapid DNA Ligation Kit (Roche Diagnostics, Mannheim) according to the manufacturer's information, and the ligation mixture was transformed by standard methods as described in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)), into competent E. coli XL-1Blue (Stratagene, La Jolla, USA). Selection for plasmid-harboring cells was achieved by plating out on LB agar containing kanamycin (20 μg/ml) (Lennox, 1955, Virology, 1:190).
The plasmid DNA of individual clones was isolated using the Qiaprep Spin Miniprep Kit (Qiagen, Hilden, Germany) according to the manufacturer's information and checked via restriction digestions. Two correct plasmids were sequenced. Sequencing reactions were carried out according to Sanger et al. (1977) Proceedings of the National Academy of Sciences USA 74:5463-5467. The sequencing reactions were fractionated and evaluated by means of ABI Prism 377 (PE Applied Biosystems, Weiterstadt). The resultant plasmid, pClik Peftu Psod ddh, is suitable for integrating the PeftuPsodcassette chromosomally upstream of the ddh ORF with the aid of two successive recombination events.
The pCIS PeftuPsod ddh plasmid is listed as SEQ ID NO: 82.
Example 17
Generation of the Strains ATCC13032 lysC fbr Peftu ddh and ATCC13032 lysC fbr PeftuPsod ddh
Cells of the E. coli strain Mn522 (Stratagene, Amsterdam, The Netherlands) were transformed with either of the plasmids pCIS Peftu ddh and pCIS PeftuPsod ddh and with the plasmid pTc15AcglM according to Liebl, et al. (1989) FEMS Microbiology Letters 53:299-303. The pTc15AcglM plasmid enables DNA to be methylated according to the Corynebacterium glutamicum methylation pattern (DE 10046870). This methylation increases the stability of the pCIS Peftu ddh and pCIS PeftuPsod ddh plasmids in C. glutamicum cells. The plasmid DNA was subsequently isolated from the Mn522 cells by standard methods and introduced with the aid of electroporation into the above-described Corynebacterium glutamicum strain ATCC 13032 ask (fbr). Said electroporation and the subsequent selection on CM plates containing kanamycin (25 μg/ml) produced a plurality of transconjugants. In order to select for the second recombination event which should excise the vector, said transconjugants were grown in CM medium without kanamycin overnight and subsequently plated out on CM plates containing 10% sucrose for selection. The sacB gene present on the pCIS vector codes for the enzyme levan sucrase and, with growth on sucrose, leads to the synthesis of levan. Since levan is toxic for C. glutamicum , only C. glutamicum cells which have lost the integration plasmid due to the second recombination step grow on sucrose-containing medium (Jäger et al., Journal of Bacteriology 174 (1992) 5462-5466). 100 sucrose-resistant clones were checked for their kanamycin sensitivity. In a plurality of the clones tested, a sensitivity to kanamycin was also detected, in addition to resistance to sucrose, which is expected with the desired excision of the vector sequences. The polymerase chain reaction (PCR) was used in order to check whether the desired integration of the Peftu or PeftuPsod expression unit had also occurred. To this end, the particular clones were removed from the agar plate using a toothpick and suspended in 100 μl of H 2 O and boiled at 95° C. for 10 min. 10 μl of the solution obtained were in each case used as template in the PCR. The primers used were oligonucleotides which are homologous to the expression unit to be introduced and the ddh gene. The PCR conditions were chosen as follows: initial denaturation: 5 min at 95° C.; denaturation: 30 at 95° C.; hybridization: 30 s at 55° C.; amplification: 2 min at 72° C.; 30 cycles: final extension: 5 min at 72° C. In the reaction mixture containing the DNA of the starting strain, the choice of oligonucleotides did not produce any PCR product. A band was expected only for clones in which the Peftu or PeftuPsod expression units had been integrated immediately 5′ of the ddh gene as a result of the 2nd recombination. The successful integration by the clones tested positive here was moreover also confirmed through Southern blot analysis (by standard methods as described, for example, in Sambrook et al. (Molecular Cloning. A Laboratory Manual, Cold Spring Harbor, (1989)). 2 different hydrolyses with restriction enzymes were carried out here for each strain to be generated, which hydrolyses would allow the starting strain (ATCC13032 ask (fbr)) and the desired strain to be distinguished unambiguously. The fact that the strains obtained do not have any kanamycin genes in the chromosome was moreover confirmed with the aid of a probe which is homologous to the kanamycin resistance gene.
Thus the following strains were generated:
ATCC13032 lysC fbr Peftu ddh
ATCC13032 lysC fbr PeftuPsod ddh
All of these strains comprise a feedback-deregulated ask gene and therefore produce lysine. They differ from one another only by the regulatory unit controlling transcription of the ddh gene.
Example 18
Effect of ddh Enhancement by a Double Promoter on Lysine Production
In order to study the effect of enhancement of the ddh gene with the aid of a double promoter, the following strains were tested for lysine production:
ATCC13032 lysCfbr
ATCC13032 lysCfbr Peftu ddh
ATCC13032 lysCfbr Peftu Psod ddh
For this purpose, the strains were grown on CM plates (10.0 g/L D-glucose, 2.5 g/L NaCl, 2.0 g/L urea, 10.0 g/L Bacto Peptone (Difco), 5.0 g/L Yeast Extract (Difco), 5.0 g/L Beef Extract (Difco), 22.0 g/L Agar (Difco), autoclaved (20 min, 121° C.)) at 30° C. for 3 days. The cells were then scraped off the plate and resuspended in saline. For the main culture, 10 ml of medium 1 and 0.5 g of autoclaved CaCO 3 (Riedel de Haen) were inoculated with the cell suspension to an OD 600 of 1.5 in a 100 ml Erlenmeyer flask and incubated on an Infors AJ118 (Infors, Bottmingen, Switzerland) at 220 rpm for 40 h. This was followed by determining the concentration of the lysine secreted into the medium.
Medium I:









40
g/l
Sucrose

60
g/l
Molasses (based on 100% sugar content)

10
g/l
(NH 4 ) 2 SO 4

0.4
g/l
MgSO 4 *7H 2 O

0.6
g/l
KH 2 PO 4

0.3
mg/l
Thiamine*HCl

1
mg/l
Biotin (from a 1 mg/ml stock solution



which has been sterilized by filtration



and adjusted to pH 8.0 with NH 4 OH)

2
mg/l
FeSO 4

2
mg/l
MnSO 4



adjusted to pH 7.8 with NH 4 OH,



autoclaved (121° C., 20 min).


























Additionally, vitamin B12 (hydroxycobalamine, Sigma Chemicals) is added from a stock solution (200 μg/ml, sterilized by filtration) to a final concentration of 100 μg/l.
The amino acid concentration was determined by means of high pressure liquid chromatography according to Agilent on an Agilent 1100 Series LC System HPLC. A guard column derivatization with ortho-phthalaldehyde allows quantification of the amino acids formed, and the amino acid mixture is fractionated on a Hypersil AA column (Agilent).
The result of the study is depicted in the table below.











Relative lysine


Strain
concentration (%)










ATCC13032 lysC fbr
100


ATCC13032 lysC fbr Peftu ddh
102.9


ATCC13032 lysC fbr PeftuPsod ddh
106.9