(en)The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. These properties include increased biomass and increased tolerance to cold, water deprivation, and low nitrogen conditions.

1.ApplicationNumber: US-66664203-A
1.PublishNumber: US-7196245-B2
2.Date Publish: 20070327
3.Inventor: JIANG CAI-ZHONG
HEARD JACQUELINE E.
RATCLIFFE OLIVER
CREELMAN ROBERT A.
RIECHMANN JOSE LUIS
HAAKE VOLKER

4.Inventor Harmonized: JIANG CAI-ZHONG(US)
HEARD JACQUELINE E(US)
RATCLIFFE OLIVER(US)
CREELMAN ROBERT A(US)
RIECHMANN JOSE LUIS(US)
HAAKE VOLKER(DE)

5.Country: US
6.Claims:
(en)The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. These properties include increased biomass and increased tolerance to cold, water deprivation, and low nitrogen conditions.

7.Description:
(en)RELATIONSHIP TO COPENDING APPLICATIONS
This application claims the benefit of copending U.S. Provisional Application No. 60/411,837, filed Sep. 18, 2002, U.S. Provisional Application No. 60/434,166, filed Dec. 17, 2002, and U.S. Provisional Application No. 60/465,809, filed Apr. 24, 2003, the entire contents of which are hereby incorporated by reference.


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CD-ROM2 (Copy 2 Replacement Mar. 11, 2004) is an exact copy of CD-ROM1 (Copy 1 Replacement Mar. 11, 2004). CD-ROM3 contains a computer readable format (CRF) copy of the Sequence Listing as a text (.txt) file.
TECHNICAL FIELD
The claimed invention, in the field of functional genomics and the characterization of plant genes for the improvement of plants, was made by or on behalf of Mendel Biotechnology, Inc. and Monsanto Corporation as a result of activities undertaken within the scope of a joint research agreement, said agreement having been in effect on or before the date the claimed invention was made.
BACKGROUND OF THE INVENTION
A plant's traits, such as its biochemical, developmental, or phenotypic characteristics, may be controlled through a number of cellular processes. One important way to manipulate that control is through transcription factors—proteins that influence the expression of a particular gene or sets of genes. Transformed and transgenic plants comprise cells having altered levels of at least one selected transcription factor, and may possess advantageous or desirable traits. Strategies for manipulating traits by altering a plant cell's transcription factor content can therefore result in plants and crops with new and/or improved commercially valuable properties.
Transcription factors can modulate gene expression, either increasing or decreasing (inducing or repressing) the rate of transcription. This modulation results in differential levels of gene expression at various developmental stages, in different tissues and cell types, and in response to different exogenous (e.g., environmental) and endogenous stimuli throughout the life cycle of the organism.
Because transcription factors are key controlling elements of biological pathways, altering the expression levels of one or more transcription factors can change entire biological pathways in an organism. For example, manipulation of the levels of selected transcription factors may result in increased expression of economically useful proteins or biomolecules in plants or improvement in other agriculturally relevant characteristics. Conversely, blocked or reduced expression of a transcription factor may reduce biosynthesis of unwanted compounds or remove an undesirable trait. Therefore, manipulating transcription factor levels in a plant offers tremendous potential in agricultural biotechnology for modifying a plant's traits. A number of the agriculturally relevant characteristics of plants, and desirable traits that may be imbued by modified transcription factor gene expression, are listed below.
Chilling Tolerance
The term “chilling sensitivity” has been used to describe many types of physiological damage produced at low, but above freezing, temperatures. Most crops of tropical origins such as soybean, rice, maize and cotton are easily damaged by chilling. Typical chilling damage includes wilting, necrosis, chlorosis or leakage of ions from cell membranes. The underlying mechanisms of chilling sensitivity are not completely understood yet, but probably involve the level of membrane saturation and other physiological deficiencies. For example, photoinhibition of photosynthesis (disruption of photosynthesis due to high light intensities) often occurs under clear atmospheric conditions subsequent to cold late summer/autumn nights. By some estimates, chilling accounts for monetary losses in the United States (US) second only to drought and flooding. For example, chilling may lead to yield losses and lower product quality through the delayed ripening of maize. Another consequence of poor growth is the rather poor ground cover of maize fields in spring, often resulting in soil erosion, increased occurrence of weeds, and reduced uptake of nutrients. A retarded uptake of mineral nitrogen could also lead to increased losses of nitrate into the ground water.
Freezing Tolerance.
Freezing is a major environmental stress that limits where crops can be grown and that reduces yields considerably, depending on the weather in a particular growing season. In addition to exceptionally stressful years that cause measurable losses of billions of dollars, less extreme stress almost certainly causes smaller yield reductions over larger areas to produce yield reductions of similar dollar value every year. For instance, in the US, the 1995 early fall frosts are estimated to have caused losses of over one billion dollars to corn and soybeans. The spring of 1998 saw an estimated $200 M of damages to Georgia alone in the peach, blueberry and strawberry industries. The occasional freezes in Florida have shifted the citrus belt further south due to $100 M or more losses. California sustained $650 M of damage in 1998 to the citrus crop due to a winter freeze. In addition, certain crops such as Eucalyptus, which has the very favorable properties of rapid growth and good wood quality for pulping, are not able to grow in the southeastern states due to occasional freezes.
Inherent winter hardiness of the crop determines in which agricultural areas it can survive the winter. For example, for wheat, the northern central portion of the US has winters that are too cold for good winter wheat crops. Approximately 20% of the US wheat crop is spring wheat, with a market value of $2 billion. Areas growing spring wheat could benefit by growing winter wheat that had increased winter hardiness. Assuming a 25% yield increase when growing winter wheat, this would create $500 M of increased value. Additionally, the existing winter wheat is severely stressed by freezing conditions and should have improved yields with increased tolerance to these stresses. An estimate of the yield benefit of these traits is 10% of the $4.4 billion winter wheat crop in the US or $444 M of yield increase, as well as better survival in extreme freezing conditions that occur periodically.
Thus, plants more resistant to freezing, both midwinter freezing and sudden freezes, would protect a farmers' investment, improve yield and quality, and allow growers in some geographies to grow more profitable and productive crops. Additionally, winter crops such as canola, wheat and barley have 25% to 50% yield increases relative to spring planted varieties of the same crops. This yield increase is due to the “head start” the fall planted crops have over the spring planted crops and their reaching maturity earlier while the temperatures, soil moisture and lack of pathogens provide more favorable conditions.
Salt Tolerance.
One in five hectares of irrigated land is damaged by salt, an important historical factor in the decline of ancient agrarian societies. This condition is only expected to worsen, further reducing the availability of arable land and crop production, since none of the top five food crops—wheat, corn, rice, potatoes, and soybean—can tolerate excessive salt.
Detrimental effects of salt on plants are a consequence of both water deficit resulting in osmotic stress (similar to drought stress) and the effects of excess sodium ions on critical biochemical processes. As with freezing and drought, high saline causes water deficit; the presence of high salt makes it difficult for plant roots to extract water from their environment (Buchanan et al. (2000) in Biochemistry and Molecular Biology of Plants , American Society of Plant Physiologists, Rockville, Md.). Soil salinity is thus one of the more important variables that determines where a plant may thrive. In many parts of the world, sizable land areas are uncultivable due to naturally high soil salinity. To compound the problem, salination of soils that are used for agricultural production is a significant and increasing problem in regions that rely heavily on agriculture. The latter is compounded by over-utilization, over-fertilization and water shortage, typically caused by climatic change and the demands of increasing population. Salt tolerance is of particular importance early in a plant's lifecycle, since evaporation from the soil surface causes upward water movement, and salt accumulates in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt level in the whole soil profile.
Drought Tolerance.
While much of the weather that we experience is brief and short-lived, drought is a more gradual phenomenon, slowly taking hold of an area and tightening its grip with time. In severe cases, drought can last for many years and can have devastating effects on agriculture and water supplies. With burgeoning population and chronic shortage of available fresh water, drought is not only the number one weather related problem in agriculture, it also ranks as one of the major natural disasters of all time, causing not only economic damage, but also loss of human lives. For example, losses from the US drought of 1988 exceeded $40 billion, exceeding the losses caused by Hurricane Andrew in 1992, the Mississippi River floods of 1993, and the San Francisco earthquake in 1989. In some areas of the world, the effects of drought can be far more severe. In the Horn of Africa the 1984–1985 drought led to a famine that killed 750,000 people.
Problems for plants caused by low water availability include mechanical stresses caused by the withdrawal of cellular water. Drought also causes plants to become more susceptible to various diseases (Simpson (1981) “The Value of Physiological Knowledge of Water Stress in Plants”, In Water Stress on Plants , (Simpson, G. M., Ed), Praeger, N.Y., pp. 235–265).
In addition to the many land regions of the world that are too arid for most if not all crop plants, overuse and over-utilization of available water is resulting in an increasing loss of agriculturally-usable land, a process which, in the extreme, results in desertification. The problem is further compounded by increasing salt accumulation in soils, as described above, which adds to the loss of available water in soils.
Water deficit is a common component of many plant stresses. Water deficit occurs in plant cells when the whole plant transpiration rate exceeds the water uptake. In addition to drought, other stresses, such as salinity and low temperature, produce cellular dehydration (McCue and Hanson (1990) Trends Biotechnol. 8: 358–362).
Salt and drought stress signal transduction consist of ionic and osmotic homeostasis signaling pathways. The ionic aspect of salt stress is signaled via the SOS pathway where a calcium-responsive SOS3–SOS2 protein kinase complex controls the expression and activity of ion transporters such as SOS1. The pathway regulating ion homeostasis in response to salt stress has been reviewed recently by Xiong and Zhu (2002) Plant Cell Environ. 25: 131–139.
The osmotic component of salt stress involves complex plant reactions that overlap with drought and/or cold stress responses.
Common aspects of drought, cold and salt stress response have been reviewed recently by Xiong and Zhu (2002) supra). Those include:
(a) transient changes in the cytoplasmic calcium levels very early in the signaling event (Knight, (2000) Int. Rev. Cytol. 195: 269–324; Sanders et al. (1999) Plant Cell 11: 691–706); (b) signal transduction via mitogen-activated and/or calcium dependent protein kinases (CDPKs; see Xiong and Zhu (2002) supra) and protein phosphatases (Merlot et al. (2001) Plant J. 25: 295–303; Tähtiharju and Palva (2001) Plant J. 26: 461–470); (c) increases in abscisic acid levels in response to stress triggering a subset of responses (Xiong and Zhu (2002) supra, and references therein); (d) inositol phosphates as signal molecules (at least for a subset of the stress responsive transcriptional changes (Xiong et al. (2001) Genes Dev. 15: 1971–1984); (e) activation of phospholipases which in turn generate a diverse array of second messenger molecules, some of which might regulate the activity of stress responsive kinases (phospholipase D functions in an ABA independent pathway, Frank et al. (2000) Plant Cell 12: 111–124); (f) induction of late embryogenesis abundant (LEA) type genes including the CRT/DRE-containing COR/RD genes (Xiong and Zhu (2002) supra); (g) increased levels of antioxidants and compatible osmolytes such as proline and soluble sugars (Hasegawa et al. (2000) Annu. Rev. Plant Mol. Plant Physiol. 51: 463–499); (h) accumulation of reactive oxygen species such as superoxide, hydrogen peroxide, and hydroxyl radicals (Hasegawa et al. (2000) supra).

Abscisic acid biosynthesis is regulated by osmotic stress at multiple steps. Both ABA-dependent and ABA-independent osmotic stress signaling first modify constitutively expressed transcription factors, leading to the expression of early response transcriptional activators, which then activate downstream stress tolerance effector genes.
Based on the commonality of many aspects of cold, drought and salt stress responses, it can be concluded that genes that increase tolerance to cold or salt stress can also improve drought stress protection. In fact this has already been demonstrated for transcription factors (in the case of AtCBF/DREB1) and for other genes such as OsCDPK7 (Saijo et al. (2000) Plant J. 23: 319–327), or AVP1 (a vacuolar pyrophosphatase-proton-pump; Gaxiola et al. (2001) Proc. Natl. Acad. Sci . USA 98: 11444–11449).
Heat Tolerance.
Germination of many crops is very sensitive to temperature. A transcription factor that would enhance germination in hot conditions would be useful for crops that are planted late in the season or in hot climates. Seedlings and mature plants that are exposed to excess heat may experience heat shock, which may arise in various organs, including leaves and particularly fruit, when transpiration is insufficient to overcome heat stress. Heat also damages cellular structures, including organelles and cytoskeleton, and impairs membrane function (Buchanan, supra).
Heat shock may result a decrease in overall protein synthesis, accompanied by expression of heat shock proteins. Heat shock proteins function as chaperones and are involved in refolding proteins denatured by heat.
Tolerance to Low Nitrogen and Phosphorus.
The ability of all plants to remove nutrients from their environment is essential to survival. Thus, identification of genes that encode polypeptides with transcription factor activity may allow for the generation of transgenic plants that are better able to make use of available nutrients in nutrient-poor environments.
Among the most important macronutrients for plant growth that have the largest impact on crop yield are nitrogenous- and phosphorus-containing compounds. Nitrogen- and phosphorus-containing fertilizers are used intensively in agriculture practices today. An increase in grain crop yields from 0.5 to 1.0 metric tons per hectare to 7 metric tons per hectare accompanied the use of commercial fixed nitrogen fertilizer in production farming (Vance (2001) Plant Physiol 127: 390–397). Given current practices, in order to meet food production demands in years to come, considerable increases in the amount of nitrogen- and phosphorus-containing fertilizers will be required (Vance, supra).
Nitrogen (N) is the most abundant element on earth yet it is one of the most limiting elements to plant growth due to its lack of availability in the soil. Plants obtain N from the soil from several sources including commercial fertilizers, manure and the mineralization of organic matter. The intensive use of N fertilizers in present agricultural practices is problematic, the energy intensive Haber-Bosch process makes N fertilizer and it is estimated that the US uses annually between 3–5% of the nation's natural gas for this process. In addition to the expense of N fertilizer production and the depletion of non-renewable resources, the use of N fertilizers has led to the eutrophication of freshwater ecosystems and the contamination of drinking water due to the runoff of excess fertilizer into ground water supplies.
Phosphorus (P) is second only to N in its importance as a macronutrient for plant growth and to its impact on crop yield. Phosphorus is extremely immobile and not readily available to roots in the soil and is therefore often growth limiting to plants. Inorganic phosphate (Pi) is a constituent of several important molecules required for energy transfer, metabolic regulation and protein activation (Marschner (1995) Mineral Nutrition of Higher Plants, 2nd ed., Academic Press, San Diego, Calif.). Plants have evolved several strategies to help cope with P and N deprivation that include metabolic as well as developmental adaptations. Most, if not all, of these strategies have components that are regulated at the level of transcription and therefore are amenable to manipulation by transcription factors. Metabolic adaptations include increasing the availability of P and N by increasing uptake from the soil though the induction of high affinity and low affinity transporters, and/or increasing P and N mobilization in the plant. Developmental adaptations include increases in primary and secondary roots, increases in root hair number and length, and associations with mycorrhizal fungi (Bates and Lynch (1996) Plant Cell Environ. 19: 529–538; Harrison (1999) Annu. Rev. Plant Physiol. Plant Mol. Biol. 50: 361–389).
Disease Resistance.
Disease management is a significant expense in crop production worldwide. According to EPA reports for 1996 and 1997, US farmers spend approximately $6 billion on fungicides annually. Despite this expenditure, according to a survey conducted by the food and agriculture organization, plant diseases still reduce worldwide crop productivity by 12% and in the United States alone, economic losses due to plant pathogens amounts to 9.1 billion dollars (FAO, 1993). Data from these reports and others demonstrate that despite the availability of chemical control only a small proportion of the losses due to disease can be prevented. Not only are fungicides and anti-bacterial treatments expensive to growers, but their widespread application poses both environmental and health risks. The use of plant biotechnology to engineer disease resistant crops has the potential to make a significant economic impact on agriculture and forestry industries in two ways: reducing the monetary and environmental expense of fungicide application and reducing both pre-harvest and post-harvest crop losses that occur now despite the use of costly disease management practices.
Fungal, bacterial, oomycete, viral, and nematode diseases of plants are ubiquitous and important problems, and often severely impact yield and quality of crop and other plants. A very few examples of diseases of plants include:
Powdery mildew, caused by the fungi Erysiphe, Sphaerotheca, Phyllactinia, Microsphaera, Podosphaera or Uncinula , in, for example, wheat, bean, cucurbit, lettuce, pea, grape, tree fruit crops, as well as roses, phlox, lilacs, grasses, and Euonymus;
Fusarium -caused diseases such as Fusarium wilt in cucurbits, Fusarium head blight in barley and wheat, wilt and crown and root rot in tomatoes;
Sudden oak death, caused by the oomycete Phytophthora ramorum ; this disease was first detected in 1995 in California tan oaks. The disease has since killed more than 100,000 tan oaks, coast live oaks, black oaks, and Shreve's oaks in coastal regions of northern California, and more recently in southwestern Oregon (Roach (2001) National Geographic News , Dec. 6, 2001);
Black Sigatoka, a fungal disease caused by Mycosphaerella species that attacks banana foliage, is spreading throughout the regions of the world that are responsible for producing most of the world's banana crop;
Eutypa dieback, caused by Eutypa lata , affects a number of crop plants, including vine grape. Eutypa dieback delays shoot emergence, and causes chlorosis, stunting, and tattering of leaves;
Pierce's disease, caused by the bacterium Xylella fastidiosa , precludes growth of grapes in the southeastern United States, and threatens the profitable wine grape industry in northern California. The bacterium clogs the vasculature of the grapevines, resulting in foliar scorching followed by slow death of the vines. There is no known treatment for Pierce's disease;
Bacterial Spot caused by the bacterium Xanthomonas campestris causes serious disease problems on tomatoes and peppers. It is a significant problem in the Florida tomato industry because it spreads rapidly, especially in warm periods where there is wind-driven rain. Under these conditions, there are no adequate control measures;
Diseases caused by viruses of the family Geminiviridae are a growing agricultural problem worldwide. Geminiviruses have caused severe crop losses in tomato, cassava, and cotton. For instance, in the 1991–1992 growing season in Florida, geminiviruses caused $140 million in damages to the tomato crop (Moffat (1991) Science 286: 1835). Geminiviruses have the ability to recombine between strains to produce new virulent varieties rapidly. Therefore, there is a pressing need for broad-spectrum geminivirus control;
The soybean cyst nematode, Heterodera glycines , causes stunting and chlorosis of soybean plants, which results in yield losses or plant death from severe infestation. Annual losses in the United States have been estimated at $1.5 billion (University of Minnesota Extension Service).
The aforementioned pathogens represent a very small fraction of diverse species that seriously affect plant health and yield. For a more complete description of numerous plant diseases, see, for example, Vidhyasekaran (1997 , Fungal Pathogenesis in Plants and Crops: Molecular Biology and Host Defense Mechanisms , Marcel Dekker, Monticello, N.Y.), or Agrios (1997 , Plant Pathology , Academic Press, New York, N.Y.). Plants that are able to resist disease may produce significantly higher yields and improved food quality. It is thus of considerable importance to find genes that reduce or prevent disease.
Reduced Shade Avoidance.
Shade avoidance describes the process in which plants grown in close proximity attempt to out-compete each other by increasing stem length at the expense of leaf, fruit and storage organ development. This is caused by the plant's response to far-red radiation reflected from leaves of neighboring plants, which is mediated by phytochrome photoreceptors. Close proximity to other plants, as is produced in high-density crop plantings, increases the relative proportion of far-red irradiation, and therefore induces the shade avoidance response. Shade avoidance adversely affects biomass and yield, particularly when leaves, fruits or other storage organs constitute the desired crop (see, for example, Smith (1982) Annu. Rev. Plant Physiol. 33: 481–518; Ballare et al. (1990) Science 247: 329–332; Smith (1995) Annu. Dev. Plant Physiol. Mol. Biol., 46: 289–315; and Schmitt et al. (1995), American Naturalist, 146: 937–953). Alteration of the shade avoidance response in tobacco through alteration of phytochrome levels has been shown to produce an increase in harvest index (leaf biomass/total biomass) at high planting density, which would result in higher yield (Robson et al. (1996) Nature Biotechnol. 14: 995–998).
Altered Flowering Time and Flowering Control.
Timing of flowering has a significant impact on production of agricultural products. For example, varieties with different flowering responses to environmental cues are necessary to adapt crops to different production regions or systems. Such a range of varieties have been developed for many crops, including wheat, corn, soybean, and strawberry. Improved methods for alteration of flowering time will facilitate the development of new, geographically adapted varieties.
Breeding programs for the development of new varieties can be limited by the seed-to-seed cycle. Thus, breeding new varieties of plants with multi-year cycles (such as biennials, e.g. carrot, or fruit trees, such as citrus) can be very slow. With respect to breeding programs, there would be a significant advantage in having commercially valuable plants that exhibit controllable and modified periods to flowering (“flowering times”). For example, accelerated flowering would shorten crop and tree breeding programs.
Improved flowering control allows more than one planting and harvest of a crop to be made within a single season. Early flowering would also improve the time to harvest plants in which the flower portion of the plant constitutes the product (e.g., broccoli, cauliflower, and other edible flowers). In addition, chemical control of flowering through induction or inhibition of flowering in plants could offer a significant advantage to growers who could provide for more uniform fruit production (e.g., in strawberry)
A sizable number of plants for which the vegetative portion of the plant forms the valuable crop tend to “bolt” dramatically (e.g., spinach, onions, lettuce), after which biomass production declines and product quality diminishes (e.g., through flowering-triggered senescence of vegetative parts). Delay or prevention of flowering may also reduce or preclude dissemination of pollen from transgenic plants.
Increased Size and Biomass.
The ability to increase the biomass or size of a plant would have several important commercial applications. Crop species may be generated that produce higher yields on larger cultivars, particularly those in which the vegetative portion of the plant is edible. For example, increasing plant leaf biomass may increase the yield of leafy vegetables for human or animal consumption. Additionally, increasing leaf biomass can be used to increase production of plant-derived pharmaceutical or industrial products. By increasing plant biomass, increased production levels of the products may be obtained from the plants. Tobacco leaves, in particular, have been employed as plant factories to generate such products. Furthermore, it may be desirable to increase crop yields of plants by increasing total plant photosynthesis. An increase in total plant photosynthesis is typically achieved by increasing leaf area of the plant. Additional photosynthetic capacity may be used to increase the yield derived from particular plant tissue, including the leaves, roots, fruits or seed. In addition, the ability to modify the biomass of the leaves may be useful for permitting the growth of a plant under decreased light intensity or under high light intensity. Modification of the biomass of another tissue, such as roots, may be useful to improve a plant's ability to grow under harsh environmental conditions, including drought or nutrient deprivation, because the roots may grow deeper into the ground. Increased biomass can also be a consequence of some strategies for increased tolerance to stresses, such as drought stress. Early in a stress response plant growth (e.g., expansion of lateral organs, increase in stem girth, etc.) can be slowed to enable the plant to activate adaptive responses. Growth rate that is less sensitive to stress-induced control can result in enhanced plant size, particularly later in development.
For some ornamental plants, the ability to provide larger varieties would be highly desirable. For many plants, including fruit-bearing trees, trees that are used for lumber production, or trees and shrubs that serve as view or wind screens, increased stature provides improved benefits in the forms of greater yield or improved screening.
Because increased yield may be quite valuable to growers, we believe that there is significant commercial opportunity for engineering pathogen tolerance or resistance using a transgenic plants with altered expression of the instant plant transcription factors. Crops so engineered will provide higher yields, and may be used to improve the appearance of ornamentals. The present invention satisfies a need in the art by providing new compositions that are useful for engineering plants with increased biomass or size, and having the potential to increase yield.
Modified Growth Rate.
For almost all commercial crops, it is desirable to use plants that establish more quickly, since seedlings and young plants are particularly susceptible to stress conditions such as salinity or disease. Since many weeds may outgrow young crops or out-compete them for nutrients, it would also be desirable to determine means for allowing young crop plants to out compete weed species. Increasing seedling growth rate (emergence) contributes to seedling vigor and allows for crops to be planted earlier in the season with less concern for losses due to environmental factors. Early planting helps add days to the critical grain-filling period and increases yield.
Providing means to speed up or slow down plant growth would also be desirable to ornamental horticulture. If such means be provided, slow growing plants may exhibit prolonged pollen-producing or fruiting period, thus improving fertilization or extending harvesting season.
Modified Senescence and Cell Death.
Premature senescence, triggered by various plant stresses, can limit production of both leaf biomass and seed yield. Transcription factor genes that suppress premature senescence or cell death in response to stresses can provide means for increasing yield, particularly for those plants for which the vegetative part of the plant represents the commercial product (e.g., spinach, lettuce).
Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. In an experimental setting, tobacco plants engineered to inhibit leaf senescence had a longer photosynthetic lifespan, and produced a 50% increase in dry weight and seed yield (Gan and Amasino (1995) Science 270: 1986–1988). Delayed flower senescence may generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry, and delayed foliar and fruit senescence could improve post-harvest shelf-life of produce.
Further, programmed cell death plays a role in other plant responses, including the resistance response to disease, and some symptoms of diseases, for example, as caused by necrotrophic pathogens such as Botrytis cinerea and Sclerotinia sclerotiorum (Dickman et al. Proc. Natl. Acad. Sci., 98: 6957–6962). Localized senescence and/or cell death can be used by plants to contain the spread of harmful microorganisms. A specific localized cell death response, the “hypersensitive response”, is a component of race-specific disease resistance mediated by plant resistance genes. The hypersensitive response is thought to help limit pathogen growth and to initiate a signal transduction pathway that leads to the induction of systemic plant defenses. Accelerated senescence may be a defense against obligate pathogens such as powdery mildew that rely on healthy plant tissue for nutrients. With regard to powdery mildew, Botrytis cinerea, Sclerotinia sclerotiorum and other pathogens, transcription factors that ameliorate cell death and/or damage may reduce the significant economic losses encountered, such as, for example, Botrytis cinerea in strawberry and grape.
Altered Sugar Sensing
Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism and photosynthesis. Sucrose, for example, is the major transport form of photosynthate and its flux through cells has been shown to affect gene expression and alter storage compound accumulation in seeds (source-sink relationships). Glucose-specific hexose-sensing has also been described in plants and is implicated in cell division and repression of “famine” genes (photosynthetic or glyoxylate cycles).
Altered Morphology
Trichomes are branched or unbranched epidermal outgrowths or hair structures on a plant. Trichomes produce a variety of secondary biochemicals such as diterpenes and waxes, the former being important as, for example, insect pheromones, and the latter as protectants against desiccation and herbivorous pests. Since diterpenes also have commercial value as flavors, aromas, pesticides and cosmetics, and potential value as anti-tumor agents and inflammation-mediating substances, they have been both products and the target of considerable research. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity. Thus, it would be advantageous to discover trichome-affecting transcription factor genes for the purpose of increasing trichome density, size, or type to produce plants that are better protected from insects or that yield higher amounts of secondary metabolites.
The ability to manipulate wax composition, amount, or distribution could modify plant tolerance to drought and low humidity or resistance to insects, as well as plant appearance. In particular, a possible application for a transcription factor gene that reduces wax production in sunflower seed coats would be to reduce fouling during seed oil processing. Antisense or co-suppression of transcription factors involved in wax biosynthesis in a tissue specific manner can be used to specifically alter wax composition, amount, or distribution in those plants and crops from which wax is either a valuable attribute or product or an undesirable constituent of plants.
In many instances, the seeds of a plant constitute a valuable crop. These include, for example, the seeds of many legumes, nuts and grains. The discovery of means for producing larger seed would provide significant value by bringing about an increase in crop yield.
Modifications to flower structure may have advantageous or deleterious effects on fertility, and could be used, for example, to decrease fertility by the absence, reduction or screening of reproductive components. This could be a desirable trait, as it could be exploited to prevent or minimize the escape of the pollen of genetically modified organisms into the environment.
Manipulation of inflorescence branching patterns may also be used to influence yield and offer the potential for more effective harvesting techniques. For example, a “self pruning” mutation of tomato results in a determinate growth pattern and facilitates mechanical harvesting (Pnueli et al. (2001) Plant Cell 13(12): 2687–2702).
Other morphological characteristics that may be desirable in plants include those of an ornamental nature. These include changes in seed color, overall color, leaf and flower shape, leaf color, leaf size, or glossiness of leaves. Changes in plant or plant part coloration, brought about by modifying, for example, anthocyanin levels, would provide novel morphological features.
Plants that produce dark leaves may have benefits for human health; flavonoids, for example, have been used to inhibit tumor growth, prevent of bone loss, and prevention lipid oxidation in animals and humans. Plants in which leaf size is increased would likely provide greater biomass, which would be particularly valuable for crops in which the vegetative portion of the plant constitutes the product. Plants with glossy leaves generally produce greater epidermal wax, which, if it could be augmented, resulted in a pleasing appearance for many ornamentals, help prevent desiccation, and resist herbivorous insects and disease-causing agents. Plants with altered inflorescence, including, for example, larger flowers or distinctive floral configurations, may have high value in the ornamental horticulture industry.
Alterations of apical dominance or plant architecture could create new plant varieties. Dwarf plants may be of potential interest to the ornamental horticulture industry, and shorter, more bushy plants may also have increased resistance to lodging.
Altered Seed Oil
The composition of seeds, particularly with respect to seed oil quantity and/or composition, is very important for the nutritional value and production of various food and feed products. Desirable improvements to oils include enhanced heat stability, improved nutritional quality through, for example, reducing the number of calories in seed, increasing the number of calories in animal feeds, or altering the ratio of saturated to unsaturated lipids comprising the oils.
Altered Seed Protein
As with seed oils, seed protein content and composition is very important for the nutritional value and production of various food and feed products. Altered protein content or concentration in seeds may be used to provide nutritional benefits and may also prolong storage capacity, increase seed pest or disease resistance, or modify germination rates. Altered amino acid composition of seeds, through altered protein composition, is also a desired objective for nutritional improvement.
Altered Prenyl Lipids.
Prenyl lipids, including the tocopherols, play a role in anchoring proteins in membranes or membranous organelles. Tocopherols have both anti-oxidant and vitamin E activity. Modified tocopherol composition of plants may thus be useful in improving membrane integrity and function, which may mitigate abiotic stresses such as heat stress. Increasing the anti-oxidant and vitamin content of plants through increased tocopherol content can provide useful human health benefits.
Altered Glucosinolate Levels
Increases or decreases in specific glucosinolates or total glucosinolate content can be desirable depending upon the particular application. For example: (i) glucosinolates are undesirable components of the oilseeds used in animal feed, since they produce toxic effects; low-glucosinolate varieties of canola have been developed to combat this problem; (ii) some glucosinolates have anti-cancer activity; thus, increasing the levels or composition of these compounds can be of use in production of nutraceuticals; and (iii) glucosinolates form part of a plant's natural defense against insects; modification of glucosinolate composition or quantity could therefore afford increased protection from herbivores. Furthermore, tissue specific promoters can be used in edible crops to ensure that these compounds accumulate specifically in particular tissues, such as the epidermis, which are not taken for human consumption.
We have identified polynucleotides encoding transcription factors, developed numerous transgenic plants using these polynucleotides, and have analyzed the plants for a variety of important traits. In so doing, we have identified important polynucleotide and polypeptide sequences for producing commercially valuable plants and crops as well as the methods for making them and using them. Other aspects and embodiments of the invention are described below and can be derived from the teachings of this disclosure as a whole.
SUMMARY OF THE INVENTION
The present invention is directed to novel recombinant polynucleotides, transgenic plants comprising the polynucleotides, and methods for producing the transgenic plants.
The recombinant polynucleotides may include any of the following sequences:
(a) the nucleotide sequences found in the sequence listing; (b) nucleotide sequences encoding polypeptides found in the sequence listing; (c) sequence variants that are at least 70% sequence identical to any of the nucleotide sequences of (a) or (b); (d) orthologous and paralogous nucleotide sequences that are at least 70% identical to any of the nucleotide sequences of (a) or (b); (e) nucleotide sequence that hybridize to any of the nucleotide sequences of (a) or (b) under stringent conditions, which may include, for example, hybridization with wash steps of 6×SSC and 65 C for ten to thirty minutes per step; and (f) nucleotide sequences encoding a polypeptide having a conserved domain required for the function of regulating transcription and altering a trait in a transgenic plant, the conserved domain being at least 70% identical with a conserved domain of a polypeptide of the invention (i.e., a polypeptide listed in the sequence listing, or encoded by any of the above nucleotide sequences).

The invention also pertains to transgenic plants that may be produced by transforming plants with any recombinant polynucleotide of the invention. Due to the function of these polynucleotides, the transgenic plant will become altered phenotypically when compared with a wild-type plant. The traits that may be altered by transforming a plant with one of the present polynucleotides are numerous and varied, and may include, for example:
increased tolerance to various abiotic stresses, including cold, heat, freezing, low nitrogen and phosphorus conditions, osmotic stresses such as drought, and high salt concentrations;
increased tolerance to disease, including fungal disease, and particularly Erysiphe, Fusarium , and Botrytis ; the present polynucleotides may be used to confer increased tolerance to multiple pathogens in transformed plants;
altered sensitivity or resistance to treatments that include glyphosate, ABA, and ACC,
altered carbon/nitrogen (C/N) sensing;
advanced or delayed flowering time;
altered floral characteristics such as flower structure, loss of flower determinacy, or reduced fertility;
altered shoot meristem development, altered stem morphology and vascular tissue structure, and altered branching patterns;
reduced apical dominance;
altered trichome density, development, or structure;
altered root development, including root mass, branching and root hairs;
altered shade avoidance;
altered seed characteristics such as size, oil content, protein content, development, ripening, germination, or prenyl lipid content;
altered leaf characteristics, including size, mass, shape, color, glossiness, prenyl lipid content and other chemical modifications;
slower or faster growth than wild-type;
altered cell differentiation, proliferation, and expansion;
altered phase change;
altered senescence, programmed cell death and necrosis,
increased plant size and/or biomass, including larger seedlings than controls; dwarfed plants; and
altered pigment, including anthocyanin, levels, in various plant tissues.
Methods for producing transgenic plants having altered traits are also encompassed by the invention. These method steps include first providing an expression vector having a recombinant polynucleotide of the invention, and at least one regulatory element flanking the polynucleotide sequence Generally, the regulatory element(s) control expression of the recombinant polynucleotide in a target plant. The expression vector is then introduced into plant cells. The plant cells are grown into plants, which are allowed to overexpress a polypeptide encoded by the recombinant polynucleotide. This overexpression results in the trait alteration, in the plant. Those plants that have altered traits are identified and selected on the basis of the desirability and degree of the altered trait.



BRIEF DESCRIPTION OF THE SEQUENCE LISTING AND FIGURES
The file of this patent contains at least one drawing executed in color. Copies of this patent with color drawing(s) will be provided by the Patent and Trademark Office upon request and payment of the necessary fee.
The Sequence Listing provides exemplary polynucleotide and polypeptide sequences of the invention. The traits associated with the use of the sequences are included in the Examples.
FIG. 1 shows a conservative estimate of phylogenetic relationships among the orders of flowering plants (modified from Angiosperm Phylogeny Group (1998) Ann. Missouri Bot. Gard. 84: 1–49). Those plants with a single cotyledon (monocots) are a monophyletic clade nested within at least two major lineages of dicots; the eudicots are further divided into rosids and asterids. Arabidopsis is a rosid eudicot classified within the order Brassicales; rice is a member of the monocot order Poales. FIG. 1 was adapted from Daly et al. ((2001) Plant Physiol. 127: 1328–1333).
FIG. 2 shows a phylogenic dendogram depicting phylogenetic relationships of higher plant taxa, including clades containing tomato and Arabidopsis ; adapted from Ku et al. (2000) Proc. Natl. Acad. Sci. 97: 9121–9126; and Chase et al. (1993) Ann. Missouri Bot. Gard. 80: 528–580.
FIGS. 3A–3B illustrate an example of an osmotic stress assay. The medium used in this root growth assay contained polyethylene glycol (PEG). After germination, the seedlings of a 35S::G47 overexpressing line (the eight seedlings on left of FIG. 3A labeled “OE.G47--22”) appeared larger and had more root growth than the four wild-type seedlings on the right. As would be predicted by the osmotic stress assay, G47 plants showed enhanced survival and drought tolerance in a soil-based drought assay, as did G2133, a paralog of G47 (see FIGS. 10A–10B ). An interesting effect of G47 overexpression is shown in FIG. 3B ; the 35S::G47 plants on the left and in the center of this photograph had short, thick, fleshy inflorescences with reduced apical dominance.
FIG. 4 demonstrates an example of the effects of an altered response to light. In a germination assay conducted on MS medium in darkness, overexpression of G354 resulted in more open and greenish cotyledons and thick hypocotyls compared to wild type (G354 overexpressors are labeled “G354-29” and wild-type “WT” in this figure). G354 overexpressors also had a drought-tolerance phenotype, as indicated in Example VIII, below. Closely related paralogs of this gene, G353 and G2839, showed a osmotic stress tolerance phenotype in a germination assay on media containing high sucrose. One line of 35S::G353 seedlings and several lines of 35S::G2839 were greener and had higher germination rates than controls. This suggests that G354 and its paralogs G353 and G2839 influence osmotic stress responses.
FIGS. 5A–5D compare Arabidopsis 35S::G1274 and wild-type control seedlings in abiotic stress assays. When grown on low nitrogen media supplemented with sucrose plus glutamine, seedlings of two G1274 overexpressing lines ( FIG. 5A ) contained less anthocyanin than the wild-type seedlings ( FIG. 5B ). The lack of anthocyanin production indicated that these lines were less stressed than control seedlings under the same conditions. G1274 overexpressors ( FIG. 5C ) and wild-type ( FIG. 5D ) were also compared in a cold germination assay, in which the overexpressors were found to be larger and greener than the controls.
FIGS. 6A–6D compare soil-based drought assays for G1274 overexpressors and wild-type control plants, which confirms the results predicted after the performance of the plate-based osmotic stress assays. 35S::G1274 lines fared much better after a period of water deprivation ( FIG. 6A ) than control plants ( FIG. 6B ). This distinction was particularly evident in the overexpressor plants after being ministered with water, said plants recovering to a healthy and vigorous state, as shown in FIG. 6C . Conversely, none of the wild-type plants seen in FIG. 6D recovered after rewatering.
FIGS. 7A–7D compare growth of Arabidopsis G1792 overexpressing seedlings and wild-type controls on a single plate (two sectors of the same plate), five days after planting. On a medium lacking nitrogen and containing 3% sucrose, the 35S::G1792 lines seen in FIG. 7A generally showed greater cotyledon expansion and root growth than the wild-type seedlings in FIG. 7B . FIG. 7C is a photograph of a single plate showing a G1792 overexpressing line (labeled G1792-12; on left) and wild-type plants (on right) five days after inoculation with Botrytis cinerea , showing the chlorosis and hyphal growth in the latter control plants but not in the former overexpressors. Similar results were obtained five days after inoculation with Erysiphe orontii (not shown) and with Fusarium oxysporum , as seen in FIG. 7D , with control plants on the right showing chlorosis, and G1792 overexpressors on the left appearing to be free of the adverse effects of infection.
FIGS. 8A–8C show results obtained with G2999 overexpressing Arabidopsis plants in high salt assays. FIG. 8A illustrates the results of root growth assays with G2999 overexpressing seedlings and controls in a high sodium chloride medium. The eight 35S::G2999 Arabidopsis seedlings on the left were larger, greener, and had more root growth than the four control seedlings on the right. Another member of the G2999 clade, G2998, also showed a salt tolerance phenotype and performed similarly in the plate-based salt stress assay seen FIG. 8B . In the latter assay 35S::G2998 seedlings appeared large and green, whereas wild-type seedlings in the control assay plate shown in FIG. 8C were small and had not yet expanded their cotyledons. As is noted below, high sodium chloride growth assays often are used to indicate osmotic stress tolerance such as drought tolerance, which was subsequently confirmed with soil-based assays conducted with G2999-overexpressing plants.
FIGS. 9A–9B compare the effects of a heat assay ( FIG. 9A ) and a high salt assay ( FIG. 9B ) on Arabidopsis wild-type and G3086-overexpressing plants. Generally, the overexpressors on the left of FIG. 9A were larger, paler, and bolted earlier than the wild type plants seen on the right in this plate. The same G3086 overexpressing lines, as exemplified by the eight seedlings on the left of FIG. 9B , were also found to be larger, greener, and had more root growth in a high salt root growth assay than control plants, including the four on the right in FIG. 9B .
FIGS. 10A–10B compare the recovery from a drought treatment in two lines of G2133 overexpressing Arabidopsis plants and wild-type controls. FIG. 10A shows plants of 35S::G2133 line 5 (left) and control plants (right). FIG. 10B shows plants of 35S::G2133 line 3 (left) and control plants (right). Each pot contained several plants grown under 24 hours light. All were deprived of water for eight days, and are shown after re-watering. All of the plants of the G2133 overexpressor lines recovered, and all of the control plants were either dead or severely and adversely affected by the drought treatment.



DETAILED DESCRIPTION OF EXEMPLARY EMBODIMENTS
In an important aspect, the present invention relates to polynucleotides and polypeptides, for example, for modifying phenotypes of plants. Throughout this disclosure, various information sources are referred to and/or are specifically incorporated. The information sources include scientific journal articles, patent documents, textbooks, and World Wide Web browser-inactive page addresses, for example. While the reference to these information sources clearly indicates that they can be used by one of skill in the art, each and every one of the information sources cited herein are specifically incorporated in their entirety, whether or not a specific mention of “incorporation by reference” is noted. The contents and teachings of each and every one of the information sources can be relied on and used to make and use embodiments of the invention.
It must be noted that as used herein and in the appended claims, the singular forms “a,” “an,” and “the” include plural reference unless the context clearly dictates otherwise. Thus, for example, a reference to “a plant” includes a plurality of such plants, and a reference to “a stress” is a reference to one or more stresses and equivalents thereof known to those skilled in the art, and so forth.
The polynucleotide sequences of the invention encode polypeptides that are members of well-known transcription factor families, including plant transcription factor families, as disclosed in Tables 4–9. Generally, the transcription factors encoded by the present sequences are involved in cell differentiation and proliferation and the regulation of growth. Accordingly, one skilled in the art would recognize that by expressing the present sequences in a plant, one may change the expression of autologous genes or induce the expression of introduced genes. By affecting the expression of similar autologous sequences in a plant that have the biological activity of the present sequences, or by introducing the present sequences into a plant, one may alter a plant's phenotype to one with improved traits. The sequences of the invention may also be used to transform a plant and introduce desirable traits not found in the wild-type cultivar or strain. Plants may then be selected for those that produce the most desirable degree of over- or under-expression of target genes of interest and coincident trait improvement.
The sequences of the present invention may be from any species, particularly plant species, in a naturally occurring form or from any source whether natural, synthetic, semi-synthetic or recombinant. The sequences of the invention may also include fragments of the present amino acid sequences. In this context, a “fragment” refers to a fragment of a polypeptide sequence which is at least 5 to about 15 amino acids in length, most preferably at least 14 amino acids, and which retain some biological activity of a transcription factor. Where “amino acid sequence” is recited to refer to an amino acid sequence of a naturally occurring protein molecule, “amino acid sequence” and like terms are not meant to limit the amino acid sequence to the complete native amino acid sequence associated with the recited protein molecule.
As one of ordinary skill in the art recognizes, transcription factors can be identified by the presence of a region or domain of structural similarity or identity to a specific consensus sequence or the presence of a specific consensus DNA-binding site or DNA-binding site motif (see, for example, Riechmann et al. (2000) Science 290: 2105–2110). The plant transcription factors may belong to one of the following transcription factor families: the AP2 (APETALA2) domain transcription factor family (Riechmann and Meyerowitz (1998) Biol. Chem. 379: 633–646); the MYB transcription factor family (ENBib; Martin and Paz-Ares (1997) Trends Genet. 13: 67–73); the MADS domain transcription factor family (Riechmann and Meyerowitz (1997) Biol. Chem. 378: 1079–1101); the WRKY protein family (Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244: 563–571); the ankyrin-repeat protein family (Zhang et al. (1992) Plant Cell 4: 1575–1588); the zinc finger protein (Z) family (Klug and Schwabe (1995) FASEB J. 9: 597–604); Takatsuji (1998) Cell. Mol. Life Sci. 54:582–596); the homeobox (HB) protein family (Buerglin (1994) in Guidebook to the Homeobox Genes , Duboule (ed.) Oxford University Press); the CAAT-element binding proteins (Forsburg and Guarente (1989) Genes Dev. 3: 1166–1178); the squamosa promoter binding proteins (SPB) (Klein et al. (1996) Mol. Gen. Genet. 1996 250: 7–16); the NAM protein family (Souer et al. (1996) Cell 85: 159–170); the IAA/AUX proteins (Abel et al. (1995) J. Mol. Biol. 251: 533–549); the HLH/MYC protein family (Littlewood et al. (1994) Prot. Profile 1: 639–709); the DNA-binding protein (DBP) family (Tucker et al. (1994) EMBO J. 13: 2994–3002); the bZIP family of transcription factors (Foster et al. (1994) FASEB J. 8: 192–200); the Box P-binding protein (the BPF-1) family (da Costa e Silva et al. (1993) Plant J. 4: 125–135); the high mobility group (HMG) family (Bustin and Reeves (1996) Prog. Nucl. Acids Res. Mol. Biol. 54: 35–100); the scarecrow (SCR) family (Di Laurenzio et al. (1996) Cell 86: 423–433); the GF14 family (Wu et al. (1997) Plant Physiol. 114: 1421–1431); the polycomb (PCOMB) family (Goodrich et al. (1997) Nature 386: 44–51); the teosinte branched (TEO) family (Luo et al. (1996) Nature 383: 794–799); the ABI3 family (Giraudat et al. (1992) Plant Cell 4: 1251–1261); the triple helix (TH) family (Dehesh et al. (1990) Science 250: 1397–1399); the EIL family (Chao et al. (1997) Cell 89: 1133–44); the AT-HOOK family (Reeves and Nissen (1990) J. Biol. Chem. 265: 8573–8582); the SIFA family (Zhou et al. (1995) Nucleic Acids Res. 23: 1165–1169); the bZIPT2 family (Lu and Ferl (1995) Plant Physiol. 109: 723); the YABBY family (Bowman et al. (1999) Development 126: 2387–96); the PAZ family (Bohmert et al. (1998) EMBO J. 17: 170–80); a family of miscellaneous (MISC) transcription factors including the DPBF family (Kim et al. (1997) Plant J. 11: 1237–1251) and the SPF1 family (Ishiguro and Nakamura (1994) Mol. Gen. Genet. 244: 563–571); the GARP family (Hall et al. (1998) Plant Cell 10: 925–936), the TUBBY family (Boggin et al (1999) Science 286: 2119–2125), the heat shock family (Wu (1995) Annu. Rev. Cell Dev. Biol. 11: 441–469), the ENBP family (Christiansen et al. (1996) Plant Mol. Biol. 32: 809–821), the RING-zinc family (Jensen et al. (1998) FEBS Letters 436: 283–287), the PDBP family (Janik et al. (1989) Virology 168: 320–329), the PCF family (Cubas et al. Plant J . (1999) 18: 215–22), the SRS(SHI-related) family (Fridborg et al. (1999) Plant Cell 11: 1019–1032), the CPP (cysteine-rich polycomb-like) family (Cvitanich et al. (2000) Proc. Natl. Acad. Sci. 97: 8163–8168), the ARF (auxin response factor) family (Ulmasov et al. (1999) Proc. Natl. Acad. Sci. 96: 5844–5849), the SWI/SNF family (Collingwood et al. (1999) J. Mol. Endocrinol. 23: 255–275), the ACBF family (Seguin et al. (1997) Plant Mol. Biol. 35: 281–291), PCGL (CG-1 like) family (da Costa e Silva et al. (1994) Plant Mol. Biol. 25: 921–924) the ARID family (Vazquez et al. (1999) Development 126: 733–742), the Jumonji family (Balciunas et al. (2000), Trends Biochem. Sci. 25: 274–276), the bZIP-NIN family (Schauser et al. (1999) Nature 402: 191–195), the E2F family (Kaelin et al. (1992) Cell 70: 351–364) and the GRF-like family (Knaap et al. (2000) Plant Physiol. 122: 695–704). As indicated by any part of the list above and as known in the art, transcription factors have been sometimes categorized by class, family, and sub-family according to their structural content and consensus DNA-binding site motif, for example. Many of the classes and many of the families and sub-families are listed here. However, the inclusion of one sub-family and not another, or the inclusion of one family and not another, does not mean that the invention does not encompass polynucleotides or polypeptides of a certain family or sub-family. The list provided here is merely an example of the types of transcription factors and the knowledge available concerning the consensus sequences and consensus DNA-binding site motifs that help define them as known to those of skill in the art (each of the references noted above are specifically incorporated herein by reference). A transcription factor may include, but is not limited to, any polypeptide that can activate or repress transcription of a single gene or a number of genes. This polypeptide group includes, but is not limited to, DNA-binding proteins, DNA-binding protein binding proteins, protein kinases, protein phosphatases, protein methyltransferases, GTP-binding proteins, and receptors, and the like.
In addition to methods for modifying a plant phenotype by employing one or more polynucleotides and polypeptides of the invention described herein, the polynucleotides and polypeptides of the invention have a variety of additional uses. These uses include their use in the recombinant production (i.e., expression) of proteins; as regulators of plant gene expression, as diagnostic probes for the presence of complementary or partially complementary nucleic acids (including for detection of natural coding nucleic acids); as substrates for further reactions, e.g., mutation reactions, PCR reactions, or the like; as substrates for cloning e.g., including digestion or ligation reactions; and for identifying exogenous or endogenous modulators of the transcription factors.
Definitions
“Nucleic acid molecule” refers to a oligonucleotide, polynucleotide or any fragment thereof. It may be DNA or RNA of genomic or synthetic origin, double-stranded or single-stranded, and combined with carbohydrate, lipids, protein, or other materials to perform a particular activity such as transformation or form a useful composition such as a peptide nucleic acid (PNA).
“Polynucleotide” is a nucleic acid molecule comprising a plurality of polymerized nucleotides, e.g., at least about 15 consecutive polymerized nucleotides, optionally at least about 30 consecutive nucleotides, at least about 50 consecutive nucleotides. A polynucleotide may be a nucleic acid, oligonucleotide, nucleotide, or any fragment thereof. In many instances, a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof. Additionally, the polynucleotide may comprise a promoter, an intron, an enhancer region, a polyadenylation site, a translation initiation site, 5′ or 3′ untranslated regions, a reporter gene, a selectable marker, or the like. The polynucleotide can be single stranded or double stranded DNA or RNA. The polynucleotide optionally comprises modified bases or a modified backbone. The polynucleotide can be, e.g., genomic DNA or RNA, a transcript (such as an mRNA), a cDNA, a PCR product, a cloned DNA, a synthetic DNA or RNA, or the like. The polynucleotide can be combined with carbohydrate, lipids, protein, or other materials to perform a particular activity such as transformation or form a useful composition such as a peptide nucleic acid (PNA). The polynucleotide can comprise a sequence in either sense or antisense orientations. “Oligonucleotide” is substantially equivalent to the terms amplimer, primer, oligomer, element, target, and probe and is preferably single stranded.
“Gene” or “gene sequence” refers to the partial or complete coding sequence of a gene, its complement, and its 5′ or 3′ untranslated regions. A gene is also a functional unit of inheritance, and in physical terms is a particular segment or sequence of nucleotides along a molecule of DNA (or RNA, in the case of RNA viruses) involved in producing a polypeptide chain. The latter may be subjected to subsequent processing such as splicing and folding to obtain a functional protein or polypeptide. A gene may be isolated, partially isolated, or be found with an organism's genome. By way of example, a transcription factor gene encodes a transcription factor polypeptide, which may be functional or require processing to function as an initiator of transcription.
Operationally, genes may be defined by the cis-trans test, a genetic test that determines whether two mutations occur in the same gene and which may be used to determine the limits of the genetically active unit (Rieger et al. (1976) Glossary of Genetics and Cytogenetics: Classical and Molecular, 4th ed., Springer Verlag. Berlin). A gene generally includes regions preceding (“leaders”; upstream) and following (“trailers”; downstream) of the coding region. A gene may also include intervening, non-coding sequences, referred to as “introns”, located between individual coding segments, referred to as “exons”. Most genes have an associated promoter region, a regulatory sequence 5′ of the transcription initiation codon (there are some genes that do not have an identifiable promoter). The function of a gene may also be regulated by enhancers, operators, and other regulatory elements.
A “recombinant polynucleotide” is a polynucleotide that is not in its native state, e.g., the polynucleotide comprises a nucleotide sequence not found in nature, or the polynucleotide is in a context other than that in which it is naturally found, e.g., separated from nucleotide sequences with which it typically is in proximity in nature, or adjacent (or contiguous with) nucleotide sequences with which it typically is not in proximity. For example, the sequence at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acid.
An “isolated polynucleotide” is a polynucleotide whether naturally occurring or recombinant, that is present outside the cell in which it is typically found in nature, whether purified or not. Optionally, an isolated polynucleotide is subject to one or more enrichment or purification procedures, e.g., cell lysis, extraction, centrifugation, precipitation, or the like.
A “polypeptide” is an amino acid sequence comprising a plurality of consecutive polymerized amino acid residues e.g., at least about 15 consecutive polymerized amino acid residues, optionally at least about 30 consecutive polymerized amino acid residues, at least about 50 consecutive polymerized amino acid residues. In many instances, a polypeptide comprises a polymerized amino acid residue sequence that is a transcription factor or a domain or portion or fragment thereof. Additionally, the polypeptide may comprise 1) a localization domain, 2) an activation domain, 3) a repression domain, 4) an oligomerization domain, or 5) a DNA-binding domain, or the like. The polypeptide optionally comprises modified amino acid residues, naturally occurring amino acid residues not encoded by a codon, non-naturally occurring amino acid residues.
“Protein” refers to an amino acid sequence, oligopeptide, peptide, polypeptide or portions thereof whether naturally occurring or synthetic.
“Portion”, as used herein, refers to any part of a protein used for any purpose, but especially for the screening of a library of molecules which specifically bind to that portion or for the production of antibodies.
A “recombinant polypeptide” is a polypeptide produced by translation of a recombinant polynucleotide. A “synthetic polypeptide” is a polypeptide created by consecutive polymerization of isolated amino acid residues using methods well known in the art. An “isolated polypeptide,” whether a naturally occurring or a recombinant polypeptide, is more enriched in (or out of) a cell than the polypeptide in its natural state in a wild-type cell, e.g., more than about 5% enriched, more than about 10% enriched, or more than about 20%, or more than about 50%, or more, enriched, i.e., alternatively denoted: 105%, 110%, 120%, 150% or more, enriched relative to wild type standardized at 100%. Such an enrichment is not the result of a natural response of a wild-type plant. Alternatively, or additionally, the isolated polypeptide is separated from other cellular components with which it is typically associated, e.g., by any of the various protein purification methods herein.
“Homology” refers to sequence similarity between a reference sequence and at least a fragment of a newly sequenced clone insert or its encoded amino acid sequence.
“Hybridization complex” refers to a complex between two nucleic acid molecules by virtue of the formation of hydrogen bonds between purines and pyrimidines.
“Identity” or “similarity” refers to sequence similarity between two polynucleotide sequences or between two polypeptide sequences, with identity being a more strict comparison. The phrases “percent identity” and “% identity” refer to the percentage of sequence similarity found in a comparison of two or more polynucleotide sequences or two or more polypeptide sequences. “Sequence similarity” refers to the percent similarity in base pair sequence (as determined by any suitable method) between two or more polynucleotide sequences. Two or more sequences can be anywhere from 0–100% similar, or any integer value therebetween. Identity or similarity can be determined by comparing a position in each sequence that may be aligned for purposes of comparison. When a position in the compared sequence is occupied by the same nucleotide base or amino acid, then the molecules are identical at that position. A degree of similarity or identity between polynucleotide sequences is a function of the number of identical or matching nucleotides at positions shared by the polynucleotide sequences. A degree of identity of polypeptide sequences is a function of the number of identical amino acids at positions shared by the polypeptide sequences. A degree of homology or similarity of polypeptide sequences is a function of the number of amino acids at positions shared by the polypeptide sequences.
The term “amino acid consensus motif” refers to the portion or subsequence of a polypeptide sequence that is substantially conserved among the polypeptide transcription factors listed in the Sequence Listing.
“Alignment” refers to a number of DNA or amino acid sequences aligned by lengthwise comparison so that components in common (i.e., nucleotide bases or amino acid residues) may be readily and graphically identified. The number of components in common is related to the homology or identity between the sequences. Alignments may be used to identify “conserved domains” and relatedness within these domains. An alignment may suitably be determined by means of computer programs known in the art, such as MacVector (1999) (Accelrys, Inc., San Diego, Calif.).
A “conserved domain” or “conserved region” as used herein refers to a region in heterologous polynucleotide or polypeptide sequences where there is a relatively high degree of sequence identity between the distinct sequences.
With respect to polynucleotides encoding presently disclosed transcription factors, a conserved region is preferably at least 10 base pairs (bp) in length.
A “conserved domain”, with respect to presently disclosed polypeptides refers to a domain within a transcription factor family that exhibits a higher degree of sequence homology, such as at least 26% sequence similarity, at least 16% sequence identity, preferably at least 40% sequence identity, preferably at least 65% sequence identity including conservative substitutions, and more preferably at least 80% sequence identity, and even more preferably at least 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 90%, or at least about 95%, or at least about 98% amino acid residue sequence identity of a polypeptide of consecutive amino acid residues. A fragment or domain can be referred to as outside a conserved domain, outside a consensus sequence, or outside a consensus DNA-binding site that is known to exist or that exists for a particular transcription factor class, family, or sub-family. In this case, the fragment or domain will not include the exact amino acids of a consensus sequence or consensus DNA-binding site of a transcription factor class, family or sub-family, or the exact amino acids of a particular transcription factor consensus sequence or consensus DNA-binding site. Furthermore, a particular fragment, region, or domain of a polypeptide, or a polynucleotide encoding a polypeptide, can be “outside a conserved domain” if all the amino acids of the fragment, region, or domain fall outside of a defined conserved domain(s) for a polypeptide or protein. Sequences having lesser degrees of identity but comparable biological activity are considered to be equivalents.
As one of ordinary skill in the art recognizes, conserved domains may be identified as regions or domains of identity to a specific consensus sequence (see, for example, Riechmann et al. (2000) supra). Thus, by using alignment methods well known in the art, the conserved domains of the plant transcription factors for each of the following may be determined: the AP2 (APETALA2) domain transcription factor family (Riechmann and Meyerowitz (1998) supra; the MYB transcription factor family (ENBib; Martin and Paz-Ares (1997) supra); the MADS domain transcription factor family (Riechmann and Meyerowitz (1997) supra; Immink et al. (2003) supra); the WRKY protein family (Ishiguro and Nakamura (1994) supra); the ankyrin-repeat protein family (Zhang et al. (1992) supra); the zinc finger protein (Z) family (Klug and Schwabe (1995) supra; Takatsuji (1998) supra); the homeobox (HB) protein family (Buerglin (1994) supra); the CAAT-element binding proteins (Forsburg and Guarente (1989) supra); the squamosa promoter binding proteins (SPB) (Klein et al. (1996) supra); the NAM protein family (Souer et al. (1996) supra); the IAA/AUX proteins (Abel et al. (1995) supra); the HLH/MYC protein family (Littlewood et al. (1994) supra); the DNA-binding protein (DBP) family (Tucker et al. (1994) supra); the bZIP family of transcription factors (Foster et al. (1994) supra); the Box P-binding protein (the BPF-1) family (da Costa e Silva et al. (1993) supra); the high mobility group (HMG) family (Bustin and Reeves (1996) supra); the scarecrow (SCR) family (Di Laurenzio et al. (1996) supra); the GF14 family (Wu et al. (1997) supra); the polycomb (PCOMB) family (Goodrich et al. (1997) supra); the teosinte branched (TEO) family (Luo et al. (1996) supra); the ABI3 family (Giraudat et al. (1992) supra); the triple helix (TH) family (Dehesh et al. (1990) supra); the EIL family (Chao et al. (1997) Cell supra); the AT-HOOK family (Reeves and Nissen (1990 supra); the SIFA family (Zhou et al. (1995) supra); the bZIPT2 family (Lu and Ferl (1995) supra); the YABBY family (Bowman et al. (1999) supra); the PAZ family (Bohmert et al. (1998) supra); a family of miscellaneous (MISC) transcription factors including the DPBF family (Kim et al. (1997) supra) and the SPF1 family (Ishiguro and Nakamura (1994) supra); the GARP family (Hall et al. (1998) supra), the TUBBY family (Boggin et al. (1999) supra), the heat shock family (Wu (1995 supra), the ENBP family (Christiansen et al. (1996) supra), the RING-zinc family (Jensen et al. (1998) supra), the PDBP family (Janik et al. (1989) supra), the PCF family (Cubas et al. (1999) supra), the SRS(SHI-related) family (Fridborg et al. (1999) supra), the CPP (cysteine-rich polycomb-like) family (Cvitanich et al. (2000) supra), the ARF (auxin response factor) family (Ulmasov et al. (1999) supra), the SWI/SNF family (Collingwood et al. (1999) supra), the ACBF family (Seguin et al. (1997) supra), PCGL (CG-1 like) family (da Costa e Silva et al. (1994) supra) the ARID family (Vazquez et al. (1999) supra), the Jumonji family, (Balciunas et al. (2000) supra), the bZIP-NIN family (Schauser et al. (1999) supra), the E2F family Kaelin et al. (1992) supra) and the GRF-like family (Knaap et al (2000) supra).
The conserved domains for each of polypeptides of SEQ ID NO: 2N, wherein N=1–335 (that is, odd SEQ ID NO: 1, 3 5, 7 . . . 759) are listed in Table 5. Also, many of the polypeptides of Table 5 have conserved domains specifically indicated by start and stop sites. A comparison of the regions of the polypeptides in SEQ ID NO: 2N, wherein N=1–335 (that is, even SEQ ID NOs: 2, 4, 6, 8 . . . 760), or of those in Table 5, allows one of skill in the art to identify conserved domain(s) for any of the polypeptides listed or referred to in this disclosure, including those in Tables 4–9.
“Complementary” refers to the natural hydrogen bonding by base pairing between purines and pyrimidines. For example, the sequence A-C-G-T (5′→3′) forms hydrogen bonds with its complements A-C-G-T (5′→3′) or A-C-G-U (5′→3′). Two single-stranded molecules may be considered partially complementary, if only some of the nucleotides bond, or “completely complementary” if all of the nucleotides bond. The degree of complementarity between nucleic acid strands affects the efficiency and strength of the hybridization and amplification reactions. “Fully complementary” refers to the case where bonding occurs between every base pair and its complement in a pair of sequences, and the two sequences have the same number of nucleotides.
The terms “highly stringent” or “highly stringent condition” refer to conditions that permit hybridization of DNA strands whose sequences are highly complementary, wherein these same conditions exclude hybridization of significantly mismatched DNAs. Polynucleotide sequences capable of hybridizing under stringent conditions with the polynucleotides of the present invention may be, for example, variants of the disclosed polynucleotide sequences, including allelic or splice variants, or sequences that encode orthologs or paralogs of presently disclosed polypeptides. Nucleic acid hybridization methods are disclosed in detail by Kashima et al. (1985) Nature 313:402–404, and Sambrook et al. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (“Sambrook”); and by Haymes et al., “Nucleic Acid Hybridization: A Practical Approach”, IRL Press, Washington, D.C. (1985), which references are incorporated herein by reference.
In general, stringency is determined by the temperature, ionic strength, and concentration of denaturing agents (e.g., formamide) used in a hybridization and washing procedure (for a more detailed description of establishing and determining stringency, see below). The degree to which two nucleic acids hybridize under various conditions of stringency is correlated with the extent of their similarity. Thus, similar nucleic acid sequences from a variety of sources, such as within a plant's genome (as in the case of paralogs) or from another plant (as in the case of orthologs) that may perform similar functions can be isolated on the basis of their ability to hybridize with known transcription factor sequences. Numerous variations are possible in the conditions and means by which nucleic acid hybridization can be performed to isolate transcription factor sequences having similarity to transcription factor sequences known in the art and are not limited to those explicitly disclosed herein. Such an approach may be used to isolate polynucleotide sequences having various degrees of similarity with disclosed transcription factor sequences, such as, for example, transcription factors having 60% identity, or more preferably greater than about 70% identity, most preferably 72% or greater identity with disclosed transcription factors.
The term “equivalog” describes members of a set of homologous proteins that are conserved with respect to function since their last common ancestor. Related proteins are grouped into equivalog families, and otherwise into protein families with other hierarchically defined homology types. This definition is provided at the Institute for Genomic Research (TIGR) world wide web (www) website, “tigr.org” under the heading “Terms associated with TIGRFAMs”.
The term “variant”, as used herein, may refer to polynucleotides or polypeptides, that differ from the presently disclosed polynucleotides or polypeptides, respectively, in sequence from each other, and as set forth below.
With regard to polynucleotide variants, differences between presently disclosed polynucleotides and polynucleotide variants are limited so that the nucleotide sequences of the former and the latter are closely similar overall and, in many regions, identical. Due to the degeneracy of the genetic code, differences between the former and latter nucleotide sequences may be silent (i.e., the amino acids encoded by the polynucleotide are the same, and the variant polynucleotide sequence encodes the same amino acid sequence as the presently disclosed polynucleotide. Variant nucleotide sequences may encode different amino acid sequences, in which case such nucleotide differences will result in amino acid substitutions, additions, deletions, insertions, truncations or fusions with respect to the similar disclosed polynucleotide sequences. These variations result in polynucleotide variants encoding polypeptides that share at least one functional characteristic. The degeneracy of the genetic code also dictates that many different variant polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing.
Also within the scope of the invention is a variant of a transcription factor nucleic acid listed in the Sequence Listing, that is, one having a sequence that differs from the one of the polynucleotide sequences in the Sequence Listing, or a complementary sequence, that encodes a functionally equivalent polypeptide (i.e., a polypeptide having some degree of equivalent or similar biological activity) but differs in sequence from the sequence in the Sequence Listing, due to degeneracy in the genetic code. Included within this definition are polymorphisms that may or may not be readily detectable using a particular oligonucleotide probe of the polynucleotide encoding polypeptide, and improper or unexpected hybridization to allelic variants, with a locus other than the normal chromosomal locus for the polynucleotide sequence encoding polypeptide.
“Allelic variant” or “polynucleotide allelic variant” refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations may be “silent” or may encode polypeptides having altered amino acid sequence. “Allelic variant” and “polypeptide allelic variant” may also be used with respect to polypeptides, and in this case the term refer to a polypeptide encoded by an allelic variant of a gene.
“Splice variant” or “polynucleotide splice variant” as used herein refers to alternative forms of RNA transcribed from a gene. Splice variation naturally occurs as a result of alternative sites being spliced within a single transcribed RNA molecule or between separately transcribed RNA molecules, and may result in several different forms of mRNA transcribed from the same gene. This, splice variants may encode polypeptides having different amino acid sequences, which may or may not have similar functions in the organism. “Splice variant” or “polypeptide splice variant” may also refer to a polypeptide encoded by a splice variant of a transcribed mRNA.
As used herein, “polynucleotide variants” may also refer to polynucleotide sequences that encode paralogs and orthologs of the presently disclosed polypeptide sequences. “Polypeptide variants” may refer to polypeptide sequences that are paralogs and orthologs of the presently disclosed polypeptide sequences.
Differences between presently disclosed polypeptides and polypeptide variants are limited so that the sequences of the former and the latter are closely similar overall and, in many regions, identical. Presently disclosed polypeptide sequences and similar polypeptide variants may differ in amino acid sequence by one or more substitutions, additions, deletions, fusions and truncations, which may be present in any combination. These differences may produce silent changes and result in a functionally equivalent transcription factor. Thus, it will be readily appreciated by those of skill in the art, that any of a variety of polynucleotide sequences is capable of encoding the transcription factors and transcription factor homolog polypeptides of the invention. A polypeptide sequence variant may have “conservative” changes, wherein a substituted amino acid has similar structural or chemical properties. Deliberate amino acid substitutions may thus be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues, as long as the functional or biological activity of the transcription factor is retained. For example, negatively charged amino acids may include aspartic acid and glutamic acid, positively charged amino acids may include lysine and arginine, and amino acids with uncharged polar head groups having similar hydrophilicity values may include leucine, isoleucine, and valine; glycine and alanine; asparagine and glutamine; serine and threonine; and phenylalanine and tyrosine (for more detail on conservative substitutions, see Table 2). More rarely, a variant may have “non-conservative” changes, e.g., replacement of a glycine with a tryptophan. Similar minor variations may also include amino acid deletions or insertions, or both. Related polypeptides may comprise, for example, additions and/or deletions of one or more N-linked or O-linked glycosylation sites, or an addition and/or a deletion of one or more cysteine residues. Guidance in determining which and how many amino acid residues may be substituted, inserted or deleted without abolishing functional or biological activity may be found using computer programs well known in the art, for example, DNASTAR software (see U.S. Pat. No. 5,840,544).
“Ligand” refers to any molecule, agent, or compound that will bind specifically to a complementary site on a nucleic acid molecule or protein. Such ligands stabilize or modulate the activity of nucleic acid molecules or proteins of the invention and may be composed of at least one of the following: inorganic and organic substances including nucleic acids, proteins, carbohydrates, fats, and lipids.
“Modulates” refers to a change in activity (biological, chemical, or immunological) or lifespan resulting from specific binding between a molecule and either a nucleic acid molecule or a protein.
The term “plant” includes whole plants, shoot vegetative organs/structures (e.g., leaves, stems and tubers), roots, flowers and floral organs/structures (e.g., bracts, sepals, petals, stamens, carpels, anthers and ovules), seed (including embryo, endosperm, and seed coat) and fruit (the mature ovary), plant tissue (e.g., vascular tissue, ground tissue, and the like) and cells (e.g., guard cells, egg cells, and the like), and progeny of same. The class of plants that can be used in the method of the invention is generally as broad as the class of higher and lower plants amenable to transformation techniques, including angiosperms (monocotyledonous and dicotyledonous plants), gymnosperms, ferns, horsetails, psilophytes, lycophytes, bryophytes, and multicellular algae. (See for example, FIG. 1 , adapted from Daly et al. (2001) Plant Physiol. 127: 1328–1333; FIG. 2 , adapted from Ku et al. (2000) Proc. Natl. Acad. Sci. 97: 9121–9126; and see also Tudge in The Variety of Life , Oxford University Press, New York, N.Y. (2000) pp. 547–606).
A “transgenic plant” refers to a plant that contains genetic material not found in a wild-type plant of the same species, variety or cultivar. The genetic material may include a transgene, an insertional mutagenesis event (such as by transposon or T-DNA insertional mutagenesis), an activation tagging sequence, a mutated sequence, a homologous recombination event or a sequence modified by chimeraplasty. Typically, the foreign genetic material has been introduced into the plant by human manipulation, but any method can be used as one of skill in the art recognizes.
A transgenic plant may contain an expression vector or cassette. The expression cassette typically comprises a polypeptide-encoding sequence operably linked (i.e., under regulatory control of) to appropriate inducible or constitutive regulatory sequences that allow for the expression of polypeptide. The expression cassette can be introduced into a plant by transformation or by breeding after transformation of a parent plant. A plant refers to a whole plant as well as to a plant part, such as seed, fruit, leaf, or root, plant tissue, plant cells or any other plant material, e.g., a plant explant, as well as to progeny thereof, and to in vitro systems that mimic biochemical or cellular components or processes in a cell.
“Control plant” refers to a plant that serves as a standard of comparison for testing the results of a treatment or genetic alteration, or the degree of altered expression of a gene or gene product. Examples of control plants include plants that are untreated, or genetically unaltered (i.e., wild-type).
“Wild type”, as used herein, refers to a cell, tissue or plant that has not been genetically modified to knock out or overexpress one or more of the presently disclosed transcription factors. Wild-type cells, tissue or plants may be used as controls to compare levels of expression and the extent and nature of trait modification with cells, tissue or plants in which transcription factor expression is altered or ectopically expressed, e.g., in that it has been knocked out or overexpressed.
“Fragment”, with respect to a polynucleotide, refers to a clone or any part of a polynucleotide molecule that retains a usable, functional characteristic. Useful fragments include oligonucleotides and polynucleotides that may be used in hybridization or amplification technologies or in the regulation of replication, transcription or translation. A polynucleotide fragment” refers to any subsequence of a polynucleotide, typically, of at least about 9 consecutive nucleotides, preferably at least about 30 nucleotides, more preferably at least about 50 nucleotides, of any of the sequences provided herein. Exemplary polynucleotide fragments are the first sixty consecutive nucleotides of the transcription factor polynucleotides listed in the Sequence Listing. Exemplary fragments also include fragments that comprise a region that encodes a conserved domain of a transcription factor.
Fragments may also include subsequences of polypeptides and protein molecules, or a subsequence of the polypeptide. Fragments may have uses in that they may have antigenic potential. In some cases, the fragment or domain is a subsequence of the polypeptide which performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide. For example, a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA-binding site or domain that binds to a DNA promoter region, an activation domain, or a domain for protein—protein interactions, and may initiate transcription. Fragments can vary in size from as few as 3 amino acids to the full length of the intact polypeptide, but are preferably at least about 30 amino acids in length and more preferably at least about 60 amino acids in length. Exemplary polypeptide fragments are the first twenty consecutive amino acids of a mammalian protein encoded by are the first twenty consecutive amino acids of the transcription factor polypeptides listed in the Sequence Listing. Exemplary fragments also include fragments that comprise a conserved domain of a transcription factor, for example, amino acid residues 11–80 of G47 (SEQ ID NO: 12), as noted in Table 5.
The invention also encompasses production of DNA sequences that encode transcription factors and transcription factor derivatives, or fragments thereof, entirely by synthetic chemistry. After production, the synthetic sequence may be inserted into any of the many available expression vectors and cell systems using reagents well known in the art. Moreover, synthetic chemistry may be used to introduce mutations into a sequence encoding transcription factors or any fragment thereof.
“Derivative” refers to the chemical modification of a nucleic acid molecule or amino acid sequence. Chemical modifications can include replacement of hydrogen by an alkyl, acyl, or amino group or glycosylation, pegylation, or any similar process that retains or enhances biological activity or lifespan of the molecule or sequence.
A “trait” refers to a physiological, morphological, biochemical, or physical characteristic of a plant or particular plant material or cell. In some instances, this characteristic is visible to the human eye, such as seed or plant size, or can be measured by biochemical techniques, such as detecting the protein, starch, or oil content of seed or leaves, or by observation of a metabolic or physiological process, e.g. by measuring uptake of carbon dioxide, or by the observation of the expression level of a gene or genes, e.g., by employing Northern analysis, RT-PCR, microarray gene expression assays, or reporter gene expression systems, or by agricultural observations such as stress tolerance, yield, or pathogen tolerance. Any technique can be used to measure the amount of, comparative level of, or difference in any selected chemical compound or macromolecule in the transgenic plants, however.
“Trait modification” refers to a detectable difference in a characteristic in a plant ectopically expressing a polynucleotide or polypeptide of the present invention relative to a plant not doing so, such as a wild-type plant. In some cases, the trait modification can be evaluated quantitatively. For example, the trait modification can entail at least about a 2% increase or decrease in an observed trait (difference), at least a 5% difference, at least about a 10% difference, at least about a 20% difference, at least about a 30%, at least about a 50%, at least about a 70%, or at least about a 100%, or an even greater difference compared with a wild-type plant. It is known that there can be a natural variation in the modified trait. Therefore, the trait modification observed entails a change of the normal distribution of the trait in the plants compared with the distribution observed in wild-type plants.
The term “transcript profile” refers to the expression levels of a set of genes in a cell in a particular state, particularly by comparison with the expression levels of that same set of genes in a cell of the same type in a reference state. For example, the transcript profile of a particular transcription factor in a suspension cell is the expression levels of a set of genes in a cell overexpressing that transcription factor compared with the expression levels of that same set of genes in a suspension cell that has normal levels of that transcription factor. The transcript profile can be presented as a list of those genes whose expression level is significantly different between the two treatments, and the difference ratios. Differences and similarities between expression levels may also be evaluated and calculated using statistical and clustering methods.
“Ectopic expression or altered expression” in reference to a polynucleotide indicates that the pattern of expression in, e.g., a transgenic plant or plant tissue, is different from the expression pattern in a wild-type plant or a reference plant of the same species. The pattern of expression may also be compared with a reference expression pattern in a wild-type plant of the same species. For example, the polynucleotide or polypeptide is expressed in a cell or tissue type other than a cell or tissue type in which the sequence is expressed in the wild-type plant, or by expression at a time other than at the time the sequence is expressed in the wild-type plant, or by a response to different inducible agents, such as hormones or environmental signals, or at different expression levels (either higher or lower) compared with those found in a wild-type plant. The term also refers to altered expression patterns that are produced by lowering the levels of expression to below the detection level or completely abolishing expression. The resulting expression pattern can be transient or stable, constitutive or inducible. In reference to a polypeptide, the term “ectopic expression or altered expression” further may relate to altered activity levels resulting from the interactions of the polypeptides with exogenous or endogenous modulators or from interactions with factors or as a result of the chemical modification of the polypeptides.
The term “overexpression” as used herein refers to a greater expression level of a gene in a plant, plant cell or plant tissue, compared to expression in a wild-type plant, cell or tissue, at any developmental or temporal stage for the gene. Overexpression can occur when, for example, the genes encoding one or more transcription factors are under the control of a strong expression signal, such as one of the promoters described herein (e.g., the cauliflower mosaic virus 35S transcription initiation region). Overexpression may occur throughout a plant or in specific tissues of the plant, depending on the promoter used, as described below.
Overexpression may take place in plant cells normally lacking expression of polypeptides functionally equivalent or identical to the present transcription factors. Overexpression may also occur in plant cells where endogenous expression of the present transcription factors or functionally equivalent molecules normally occurs, but such normal expression is at a lower level. Overexpression thus results in a greater than normal production, or “overproduction” of the transcription factor in the plant, cell or tissue.
The term “phase change” refers to a plant's progression from embryo to adult, and, by some definitions, the transition wherein flowering plants gain reproductive competency. It is believed that phase change occurs either after a certain number of cell divisions in the shoot apex of a developing plant, or when the shoot apex achieves a particular distance from the roots. Thus, altering the timing of phase changes may affect a plant's size, which, in turn, may affect yield and biomass.
“Tolerance” results from specific, heritable characteristics of a host plant that allow a pathogen to develop and multiply in the host while the host, either by lacking receptor sites for, or by inactivating or compensating for the irritant secretions of the pathogen, still manages to thrive or, in the case of crop plants, produce a good crop. Tolerant plants are susceptible to the pathogen but are not killed by it and generally show little damage from the pathogen (Agrios (1988) Plant Pathology, 3rd ed. Academic Press, N.Y., p. 129).
“Resistance”, also referred to as “true resistance”, results when a plant contains one or more genes that make the plant and a potential pathogen more or less incompatible with each other, either because of a lack of chemical recognition between the host and the pathogen, or because the host plant can defend itself against the pathogen by defense mechanisms already present or activated in response to infection (Agrios (1988)) Plant Pathology, 3rd ed. Academic Press, N.Y., p. 125).
A “sample” with respect to a material containing nucleic acid molecules may comprise a bodily fluid; an extract from a cell, chromosome, organelle, or membrane isolated from a cell; genomic DNA, RNA, or cDNA in solution or bound to a substrate; a cell; a tissue; a tissue print; a forensic sample; and the like. In this context “substrate” refers to any rigid or semi-rigid support to which nucleic acid molecules or proteins are bound and includes membranes, filters, chips, slides, wafers, fibers, magnetic or nonmagnetic beads, gels, capillaries or other tubing, plates, polymers, and microparticles with a variety of surface forms including wells, trenches, pins, channels and pores. A substrate may also refer to a reactant in a chemical or biological reaction, or a substance acted upon (e.g., by an enzyme).
“Substantially purified” refers to nucleic acid molecules or proteins that are removed from their natural environment and are isolated or separated, and are at least about 60% free, preferably about 75% free, and most preferably about 90% free, from other components with which they are naturally associated.
Traits that May be Modified in Overexpressing or Knock-Out Plants
Trait modifications of particular interest include those to seed (such as embryo or endosperm), fruit, root, flower, leaf, stem, shoot, seedling or the like, including: enhanced tolerance to environmental conditions including freezing, chilling, heat, drought, water saturation, radiation and ozone; improved tolerance to microbial, fungal or viral diseases; improved tolerance to pest infestations, including insects, nematodes, mollicutes, parasitic higher plants or the like; decreased herbicide sensitivity; improved tolerance of heavy metals or enhanced ability to take up heavy metals; improved growth under poor photoconditions (e.g., low light and/or short day length), or changes in expression levels of genes of interest. Other phenotype that can be modified relate to the production of plant metabolites, such as variations in the production of taxol, tocopherol, tocotrienol, sterols, phytosterols, vitamins, wax monomers, anti-oxidants, amino acids, lignins, cellulose, tannins, prenyllipids (such as chlorophylls and carotenoids), glucosinolates, and terpenoids, enhanced or compositionally altered protein or oil production (especially in seeds), or modified sugar (insoluble or soluble) and/or starch composition. Physical plant characteristics that can be modified include cell development (such as the number of trichomes), fruit and seed size and number, yields of plant parts such as stems, leaves, inflorescences, and roots, the stability of the seeds during storage, characteristics of the seed pod (e.g., susceptibility to shattering), root hair length and quantity, internode distances, or the quality of seed coat. Plant growth characteristics that can be modified include growth rate, germination rate of seeds, vigor of plants and seedlings, leaf and flower senescence, male sterility, apomixis, flowering time, flower abscission, rate of nitrogen uptake, osmotic sensitivity to soluble sugar concentrations, biomass or transpiration characteristics, as well as plant architecture characteristics such as apical dominance, branching patterns, number of organs, organ identity, organ shape or size.
Transcription Factors Modify Expression of Endogenous Genes
Expression of genes that encode transcription factors that modify expression of endogenous genes, polynucleotides, and proteins are well known in the art. In addition, transgenic plants comprising isolated polynucleotides encoding transcription factors may also modify expression of endogenous genes, polynucleotides, and proteins. Examples include Peng et al. (1997 , Genes Development 11: 3194–3205) and Peng et al. (1999 , Nature, 400: 256–261). In addition, many others have demonstrated that an Arabidopsis transcription factor expressed in an exogenous plant species elicits the same or very similar phenotypic response. See, for example, Fu et al. (2001 , Plant Cell 13: 1791–1802); Nandi et al. (2000 , Curr. Biol. 10: 215–218); Coupland (1995 , Nature 377: 482–483); and Weigel and Nilsson (1995 , Nature 377: 482–500).
In another example, Mandel et al. (1992 , Cell 71–133-143) and Suzuki et al. (2001 , Plant J. 28: 409–418) teach that a transcription factor expressed in another plant species elicits the same or very similar phenotypic response of the endogenous sequence, as often predicted in earlier studies of Arabidopsis transcription factors in Arabidopsis (see Mandel et al. 1992, supra; Suzuki et al. 2001, supra).
Other examples include Müller et al. (2001 , Plant J. 28: 169–179); Kim et al. (2001 , Plant J. 25: 247–259); Kyozuka and Shimamoto (2002 , Plant Cell Physiol. 43: 130–135); Boss and Thomas (2002 , Nature, 416: 847–850); He et al. (2000 , Transgenic Res. 9: 223–227); and Robson et al. (2001 , Plant J. 28: 619–631).
In yet another example, Gilmour et al. (1998 , Plant J. 16: 433–442) teach an Arabidopsis AP2 transcription factor, CBF1 (SEQ ID NO: 2239), which, when overexpressed in transgenic plants, increases plant freezing tolerance. Jaglo et al. (2001 , Plant Physiol. 127: 910–917) further identified sequences in Brassica napus which encode CBF-like genes and that transcripts for these genes accumulated rapidly in response to low temperature. Transcripts encoding CBF-like proteins were also found to accumulate rapidly in response to low temperature in wheat, as well as in tomato. An alignment of the CBF proteins from Arabidopsis, B. napus , wheat, rye, and tomato revealed the presence of conserved consecutive amino acid residues, PKK/RPAGRxKFxETRHP and DSAWR, that bracket the AP2/EREBP DNA binding domains of the proteins and distinguish them from other members of the AP2/EREBP protein family. (See Jaglo et al. supra.)
Transcription factors mediate cellular responses and control traits through altered expression of genes containing cis-acting nucleotide sequences that are targets of the introduced transcription factor. It is well appreciated in the Art that the effect of a transcription factor on cellular responses or a cellular trait is determined by the particular genes whose expression is either directly or indirectly (e.g., by a cascade of transcription factor binding events and transcriptional changes) altered by transcription factor binding. In a global analysis of transcription comparing a standard condition with one in which a transcription factor is overexpressed, the resulting transcript profile associated with transcription factor overexpression is related to the trait or cellular process controlled by that transcription factor. For example, the PAP2 gene (and other genes in the MYB family) have been shown to control anthocyanin biosynthesis through regulation of the expression of genes known to be involved in the anthocyanin biosynthetic pathway (Bruce et al. (2000) Plant Cell 12: 65–79; and Borevitz et al. (2000) Plant Cell 12: 2383–2393). Further, global transcript profiles have been used successfully as diagnostic tools for specific cellular states (e.g., cancerous vs. non-cancerous; Bhattacharjee et al. (2001) Proc. Natl. Acad. Sci. USA 98: 13790–13795; and Xu et al. (2001) Proc Natl Acad Sci, USA 98: 15089–15094). Consequently, it is evident to one skilled in the art that similarity of transcript profile upon overexpression of different transcription factors would indicate similarity of transcription factor function.
Polypeptides and Polynucleotides of the Invention
The present invention provides, among other things, transcription factors (TFs), and transcription factor homolog polypeptides, and isolated or recombinant polynucleotides encoding the polypeptides, or novel sequence variant polypeptides or polynucleotides encoding novel variants of transcription factors derived from the specific sequences provided here. These polypeptides and polynucleotides may be employed to modify a plant's characteristics.
Exemplary polynucleotides encoding the polypeptides of the invention were identified in the Arabidopsis thaliana GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. In addition, further exemplary polynucleotides encoding the polypeptides of the invention were identified in the plant GenBank database using publicly available sequence analysis programs and parameters. Sequences initially identified were then further characterized to identify sequences comprising specified sequence strings corresponding to sequence motifs present in families of known transcription factors. Polynucleotide sequences meeting such criteria were confirmed as transcription factors.
Additional polynucleotides of the invention were identified by screening Arabidopsis thaliana and/or other plant cDNA libraries with probes corresponding to known transcription factors under low stringency hybridization conditions. Additional sequences, including full length coding sequences were subsequently recovered by the rapid amplification of cDNA ends (RACE) procedure, using a commercially available kit according to the manufacturer's instructions. Where necessary, multiple rounds of RACE are performed to isolate 5′ and 3′ ends. The full-length cDNA was then recovered by a routine end-to-end polymerase chain reaction (PCR) using primers specific to the isolated 5′ and 3′ ends. Exemplary sequences are provided in the Sequence Listing.
The polynucleotides of the invention can be or were ectopically expressed in overexpressor or knockout plants and the changes in the characteristic(s) or trait(s) of the plants observed. Therefore, the polynucleotides and polypeptides can be employed to improve the characteristics of plants.
The polynucleotides of the invention can be or were ectopically expressed in overexpressor plant cells and the changes in the expression levels of a number of genes, polynucleotides, and/or proteins of the plant cells observed. Therefore, the polynucleotides and polypeptides can be employed to change expression levels of a genes, polynucleotides, and/or proteins of plants.
Producing Polypeptides
The polynucleotides of the invention include sequences that encode transcription factors and transcription factor homolog polypeptides and sequences complementary thereto, as well as unique fragments of coding sequence, or sequence complementary thereto. Such polynucleotides can be, e.g., DNA or RNA, e.g., mRNA, cRNA, synthetic RNA, genomic DNA, cDNA synthetic DNA, oligonucleotides, etc. The polynucleotides are either double-stranded or single-stranded, and include either, or both sense (i.e., coding) sequences and antisense (i.e., non-coding, complementary) sequences. The polynucleotides include the coding sequence of a transcription factor, or transcription factor homolog polypeptide, in isolation, in combination with additional coding sequences (e.g., a purification tag, a localization signal, as a fusion-protein, as a pre-protein, or the like), in combination with non-coding sequences (e.g., introns or inteins, regulatory elements such as promoters, enhancers, terminators, and the like), and/or in a vector or host environment in which the polynucleotide encoding a transcription factor or transcription factor homolog polypeptide is an endogenous or exogenous gene.
A variety of methods exist for producing the polynucleotides of the invention. Procedures for identifying and isolating DNA clones are well known to those of skill in the art, and are described in, e.g., Berger and Kimmel, Guide to Molecular Cloning Techniques, Methods in Enzymology , vol. 152 Academic Press, Inc., San Diego, Calif. (“Berger”); Sambrook et al. Molecular Cloning—A Laboratory Manual (2nd Ed.), Vol. 1–3, Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989 (“Sambrook”) and Current Protocols in Molecular Biology , Ausubel et al. eds., Current Protocols, a joint venture between Greene Publishing Associates, Inc. and John Wiley & Sons, Inc., (supplemented through 2000) (“Ausubel”).
Alternatively, polynucleotides of the invention, can be produced by a variety of in vitro amplification methods adapted to the present invention by appropriate selection of specific or degenerate primers. Examples of protocols sufficient to direct persons of skill through in vitro amplification methods, including the polymerase chain reaction (PCR) the ligase chain reaction (LCR), Qbeta-replicase amplification and other RNA polymerase mediated techniques (e.g., NASBA), e.g., for the production of the homologous nucleic acids of the invention are found in Berger (supra), Sambrook (supra), and Ausubel (supra), as well as Mullis et al. (1987) PCR Protocols A Guide to Methods and Applications (Innis et al. eds) Academic Press Inc. San Diego, Calif. (1990) (Innis). Improved methods for cloning in vitro amplified nucleic acids are described in Wallace et al. U.S. Pat. No. 5,426,039. Improved methods for amplifying large nucleic acids by PCR are summarized in Cheng et al. (1994) Nature 369: 684–685 and the references cited therein, in which PCR amplicons of up to 40 kb are generated. One of skill will appreciate that essentially any RNA can be converted into a double stranded DNA suitable for restriction digestion, PCR expansion and sequencing using reverse transcriptase and a polymerase. See, e.g., Ausubel, Sambrook and Berger, all supra.
Alternatively, polynucleotides and oligonucleotides of the invention can be assembled from fragments produced by solid-phase synthesis methods. Typically, fragments of up to approximately 100 bases are individually synthesized and then enzymatically or chemically ligated to produce a desired sequence, e.g., a polynucleotide encoding all or part of a transcription factor. For example, chemical synthesis using the phosphoramidite method is described, e.g., by Beaucage et al. (1981) Tetrahedron Letters 22: 1859–1869; and Matthes et al. (1984) EMBO J. 3: 801–805. According to such methods, oligonucleotides are synthesized, purified, annealed to their complementary strand, ligated and then optionally cloned into suitable vectors. And if so desired, the polynucleotides and polypeptides of the invention can be custom ordered from any of a number of commercial suppliers.
Homologous Sequences
Sequences homologous, i.e., that share significant sequence identity or similarity, to those provided in the Sequence Listing, derived from Arabidopsis thaliana or from other plants of choice, are also an aspect of the invention. Homologous sequences can be derived from any plant including monocots and dicots and in particular agriculturally important plant species, including but not limited to, crops such as soybean, wheat, corn (maize), potato, cotton, rice, rape, oilseed rape (including canola), sunflower, alfalfa, clover, sugarcane, and turf; or fruits and vegetables, such as banana, blackberry, blueberry, strawberry, and raspberry, cantaloupe, carrot, cauliflower, coffee, cucumber, eggplant, grapes, honeydew, lettuce, mango, melon, onion, papaya, peas, peppers, pineapple, pumpkin, spinach, squash, sweet corn, tobacco, tomato, tomatillo, watermelon, rosaceous fruits (such as apple, peach, pear, cherry and plum) and vegetable brassicas (such as broccoli, cabbage, cauliflower, Brussels sprouts, and kohlrabi). Other crops, including fruits and vegetables, whose phenotype can be changed and which comprise homologous sequences include barley; rye; millet; sorghum; currant; avocado; citrus fruits such as oranges, lemons, grapefruit and tangerines, artichoke, cherries; nuts such as the walnut and peanut; endive; leek; roots such as arrowroot, beet, cassava, turnip, radish, yam, and sweet potato; and beans. The homologous sequences may also be derived from woody species, such pine, poplar and eucalyptus, or mint or other labiates. In addition, homologous sequences may be derived from plants that are evolutionarily-related to crop plants, but which may not have yet been used as crop plants. Examples include deadly nightshade ( Atropa belladona ), related to tomato; jimson weed (Datura strommium), related to peyote; and teosinte ( Zea species), related to corn (maize).
Orthologs and Paralogs
Homologous sequences as described above can comprise orthologous or paralogous sequences. Several different methods are known by those of skill in the art for identifying and defining these functionally homologous sequences. Three general methods for defining orthologs and paralogs are described; an ortholog, paralog or homolog may be identified by one or more of the methods described below.
Orthologs and paralogs are evolutionarily related genes that have similar sequence and similar functions. Orthologs are structurally related genes in different species that are derived by a speciation event. Paralogs are structurally related genes within a single species that are derived by a duplication event.
Within a single plant species, gene duplication may cause two copies of a particular gene, giving rise to two or more genes with similar sequence and often similar function known as paralogs. A paralog is therefore a similar gene formed by duplication within the same species. Paralogs typically cluster together or in the same clade (a group of similar genes) when a gene family phylogeny is analyzed using programs such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22: 4673–4680; Higgins et al. (1996) Methods Enzymol. 266: 383–402). Groups of similar genes can also be identified with pair-wise BLAST analysis (Feng and Doolittle (1987) J. Mol. Evol. 25: 351–360). For example, a clade of very similar MADS domain transcription factors from Arabidopsis all share a common function in flowering time (Ratcliffe et al. (2001) Plant Physiol. 126: 122–132), and a group of very similar AP2 domain transcription factors from Arabidopsis are involved in tolerance of plants to freezing (Gilmour et al. (1998) Plant J. 16: 433–442). Analysis of groups of similar genes with similar function that fall within one clade can yield sub-sequences that are particular to the clade. These sub-sequences, known as consensus sequences, can not only be used to define the sequences within each clade, but define the functions of these genes; genes within a clade may contain paralogous sequences, or orthologous sequences that share the same function (see also, for example, Mount (2001), in Bioinformatics: Sequence and Genome Analysis Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., page 543.)
Speciation, the production of new species from a parental species, can also give rise to two or more genes with similar sequence and similar function. These genes, termed orthologs, often have an identical function within their host plants and are often interchangeable between species without losing function. Because plants have common ancestors, many genes in any plant species will have a corresponding orthologous gene in another plant species. Once a phylogenic tree for a gene family of one species has been constructed using a program such as CLUSTAL (Thompson et al. (1994) Nucleic Acids Res. 22: 4673–4680; Higgins et al. (1996) supra) potential orthologous sequences can be placed into the phylogenetic tree and their relationship to genes from the species of interest can be determined. Orthologous sequences can also be identified by a reciprocal BLAST strategy. Once an orthologous sequence has been identified, the function of the ortholog can be deduced from the identified function of the reference sequence.
Transcription factor gene sequences are conserved across diverse eukaryotic species lines (Goodrich et al. (1993) Cell 75: 519–530; Lin et al. (1991) Nature 353: 569–571; Sadowski et al. (1988) Nature 335: 563–564). Plants are no exception to this observation; diverse plant species possess transcription factors that have similar sequences and functions.
Orthologous genes from different organisms have highly conserved functions, and very often essentially identical functions (Lee et al. (2002) Genome Res. 12: 493–502; Remm et al. (2001) J. Mol. Biol. 314: 1041–1052). Paralogous genes, which have diverged through gene duplication, may retain similar functions of the encoded proteins. In such cases, paralogs can be used interchangeably with respect to certain embodiments of the instant invention (for example, transgenic expression of a coding sequence). An example of such highly related paralogs is the CBF family, with three well-defined members in Arabidopsis and at least one ortholog in Brassica napus (SEQ ID NOs: 2238, 2240, 2242, and 2244, respectively), all of which control pathways involved in both freezing and drought stress (Gilmour et al. (1998) Plant J. 16: 433–442; Jaglo et al. (1998) Plant Physiol. 127: 910–917).
The following references represent a small sampling of the many studies that demonstrate that conserved transcription factor genes from diverse species are likely to function similarly (i.e., regulate similar target sequences and control the same traits), and that transcription factors may be transformed into diverse species to confer or improve traits.
(1) The Arabidopsis NPR1 gene regulates systemic acquired resistance (SAR); over-expression of NPR1 leads to enhanced resistance in Arabidopsis . When either Arabidopsis NPR1 or the rice NPR1 ortholog was overexpressed in rice (which, as a monocot, is diverse from Arabidopsis ), challenge with the rice bacterial blight pathogen Xanthomonas oryzae pv. Oryzae , the transgenic plants displayed enhanced resistance (Chern et al. (2001) Plant J. 27: 101–113). NPR1 acts through activation of expression of transcription factor genes, such as TGA2 (Fan and Dong (2002) Plant Cell 14: 1377–1389).
(2) E2F genes are involved in transcription of plant genes for proliferating cell nuclear antigen (PCNA). Plant E2Fs share a high degree of similarity in amino acid sequence between monocots and dicots, and are even similar to the conserved domains of the animal E2Fs. Such conservation indicates a functional similarity between plant and animal E2Fs. E2F transcription factors that regulate meristem development act through common cis-elements, and regulate related (PCNA) genes (Kosugi and Ohashi, (2002) Plant J. 29: 45–59).
(3) The ABI5 gene (ABA insensitive 5) encodes a basic leucine zipper factor required for ABA response in the seed and vegetative tissues. Co-transformation experiments with ABI5 cDNA constructs in rice protoplasts resulted in specific transactivation of the ABA-inducible wheat, Arabidopsis , bean, and barley promoters. These results demonstrate that sequentially similar ABI5 transcription factors are key targets of a conserved ABA signaling pathway in diverse plants. (Gampala et al. (2001) J. Biol. Chem. 277: 1689–1694).
(4) Sequences of three Arabidopsis GAMYB-like genes were obtained on the basis of sequence similarity to GAMYB genes from barley, rice, and L. temulentum . These three Arabidopsis genes were determined to encode transcription factors (AtMYB33, AtMYB65, and AtMYB101) and could substitute for a barley GAMYB and control alpha-amylase expression (Gocal et al. (2001) Plant Physiol. 127: 1682–1693).
(5) The floral control gene LEAFY from Arabidopsis can dramatically accelerate flowering in numerous dictoyledonous plants. Constitutive expression of Arabidopsis LEAFY also caused early flowering in transgenic rice (a monocot), with a heading date that was 26–34 days earlier than that of wild-type plants. These observations indicate that floral regulatory genes from Arabidopsis are useful tools for heading date improvement in cereal crops (He et al. (2000) Transgenic Res. 9: 223–227).
(6) Bioactive gibberellins (GAs) are essential endogenous regulators of plant growth. GA signaling tends to be conserved across the plant kingdom. GA signaling is mediated via GAI, a nuclear member of the GRAS family of plant transcription factors. Arabidopsis GAI has been shown to function in rice to inhibit gibberellin response pathways (Fu et al. (2001) Plant Cell 13: 1791–1802).
(7) The Arabidopsis gene SUPERMAN (SUP), encodes a putative transcription factor that maintains the boundary between stamens and carpels. By over-expressing Arabidopsis SUP in rice, the effect of the gene's presence on whorl boundaries was shown to be conserved. This demonstrated that SUP is a conserved regulator of floral whorl boundaries and affects cell proliferation (Nandi et al. (2000) Curr. Biol. 10: 215–218).
(8) Maize, petunia and Arabidopsis myb transcription factors that regulate flavonoid biosynthesis are very genetically similar and affect the same trait in their native species, therefore sequence and function of these myb transcription factors correlate with each other in these diverse species (Borevitz et al. (2000) Plant Cell 12: 2383–2394).
(9) Wheat reduced height-1 (Rht-B1/Rht-D1) and maize dwarf-8 (d8) genes are orthologs of the Arabidopsis gibberellin insensitive (GAI) gene. Both of these genes have been used to produce dwarf grain varieties that have improved grain yield. These genes encode proteins that resemble nuclear transcription factors and contain an SH2-like domain, indicating that phosphotyrosine may participate in gibberellin signaling. Transgenic rice plants containing a mutant GAI allele from Arabidopsis have been shown to produce reduced responses to gibberellin and are dwarfed, indicating that mutant GAI orthologs could be used to increase yield in a wide range of crop species (Peng et al. (1999) Nature 400: 256–261).
Transcription factors that are homologous to the listed sequences will typically share, in at least one conserved domain, at least about 70% amino acid sequence identity, and with regard to zinc finger transcription factors, at least about 50% amino acid sequence identity. More closely related transcription factors can share at least about 70%, or about 75% or about 80% or about 90% or about 95% or about 98% or more sequence identity with the listed sequences, or with the listed sequences but excluding or outside a known consensus sequence or consensus DNA-binding site, or with the listed sequences excluding one or all conserved domain. Factors that are most closely related to the listed sequences share, e.g., at least about 85%, about 90% or about 95% or more % sequence identity to the listed sequences, or to the listed sequences but excluding or outside a known consensus sequence or consensus DNA-binding site or outside one or all conserved domain. At the nucleotide level, the sequences will typically share at least about 40% nucleotide sequence identity, preferably at least about 50%, about 60%, about 70% or about 80% sequence identity, and more preferably about 85%, about 90%, about 95% or about 97% or more sequence identity to one or more of the listed sequences, or to a listed sequence but excluding or outside a known consensus sequence or consensus DNA-binding site, or outside one or all conserved domain. The degeneracy of the genetic code enables major variations in the nucleotide sequence of a polynucleotide while maintaining the amino acid sequence of the encoded protein. Conserved domains within a transcription factor family may exhibit a higher degree of sequence homology, such as at least 65% amino acid sequence identity including conservative substitutions, and preferably at least 80% sequence identity, and more preferably at least 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 90%, or at least about 95%, or at least about 98% sequence identity. Transcription factors that are homologous to the listed sequences should share at least 30%, or at least about 60%, or at least about 75%, or at least about 80%, or at least about 90%, or at least about 95% amino acid sequence identity over the entire length of the polypeptide or the homolog.
Percent identity can be determined electronically, e.g., by using the MEGALIGN program (DNASTAR, Inc. Madison, Wis.). The MEGALIGN program can create alignments between two or more sequences according to different methods, for example, the clustal method. (See, for example, Higgins and Sharp (1988) Gene 73: 237–244.) The clustal algorithm groups sequences into clusters by examining the distances between all pairs. The clusters are aligned pairwise and then in groups. Other alignment algorithms or programs may be used, including FASTA, BLAST, or ENTREZ, FASTA and BLAST, and which may be used to calculate percent similarity. These are available as a part of the GCG sequence analysis package (University of Wisconsin, Madison, Wis.), and can be used with or without default settings. ENTREZ is available through the National Center for Biotechnology Information. In one embodiment, the percent identity of two sequences can be determined by the GCG program with a gap weight of 1, e.g., each amino acid gap is weighted as if it were a single amino acid or nucleotide mismatch between the two sequences (see U.S. Pat. No. 6,262,333).
Other techniques for alignment are described in Methods in Enzymology, vol. 266 , Computer Methods for Macromolecular Sequence Analysis (1996), ed. Doolittle, Academic Press, Inc., San Diego, Calif., USA. Preferably, an alignment program that permits gaps in the sequence is utilized to align the sequences. The Smith-Waterman is one type of algorithm that permits gaps in sequence alignments (see Shpaer (1997) Methods Mol. Biol. 70: 173–187). Also, the GAP program using the Needleman and Wunsch alignment method can be utilized to align sequences. An alternative search strategy uses MPSRCH software, which runs on a MASPAR computer. MPSRCH uses a Smith-Waterman algorithm to score sequences on a massively parallel computer. This approach improves ability to pick up distantly related matches, and is especially tolerant of small gaps and nucleotide sequence errors. Nucleic acid-encoded amino acid sequences can be used to search both protein and DNA databases.
The percentage similarity between two polypeptide sequences, e.g., sequence A and sequence B, is calculated by dividing the length of sequence A, minus the number of gap residues in sequence A, minus the number of gap residues in sequence B, into the sum of the residue matches between sequence A and sequence B, times one hundred. Gaps of low or of no similarity between the two amino acid sequences are not included in determining percentage similarity. Percent identity between polynucleotide sequences can also be counted or calculated by other methods known in the art, e.g., the Jotun Hein method. (See, e.g., Hein (1990) Methods Enzymol. 183: 626–645.) Identity between sequences can also be determined by other methods known in the art, e.g., by varying hybridization conditions (see US Patent Application No. 20010010913).
The percent identity between two conserved domains of a transcription factor DNA-binding domain consensus polypeptide sequence can be as low as 16%, as exemplified in the case of GATA1 family of eukaryotic Cys 2 /Cys 2 -type zinc finger transcription factors. The DNA-binding domain consensus polypeptide sequence of the GATA1 family is CX 2 CX 17 CX 2 C, where X is any amino acid residue. (See, for example, Takatsuji, supra.) Other examples of such conserved consensus polypeptide sequences with low overall percent sequence identity are well known to those of skill in the art.
Thus, the invention provides methods for identifying a sequence similar or paralogous or orthologous or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an internet or intranet) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.
In addition, one or more polynucleotide sequences or one or more polypeptides encoded by the polynucleotide sequences may be used to search against a BLOCKS (Bairoch et al. (1997) Nucleic Acids Res. 25: 217–221), PFAM, and other databases which contain previously identified and annotated motifs, sequences and gene functions. Methods that search for primary sequence patterns with secondary structure gap penalties (Smith et al. (1992) Protein Engineering 5: 35–51) as well as algorithms such as Basic Local Alignment Search Tool (BLAST; Altschul (1993) J. Mol. Evol. 36: 290–300; Altschul et al. (1990) supra), BLOCKS (Henikoff and Henikoff (1991) Nucleic Acids Res. 19: 6565–6572), Hidden Markov Models (HMM; Eddy (1996) Curr. Opin. Str. Biol. 6: 361–365; Sonnhammer et al. (1997) Proteins 28: 405–420), and the like, can be used to manipulate and analyze polynucleotide and polypeptide sequences encoded by polynucleotides. These databases, algorithms and other methods are well known in the art and are described in Ausubel et al. (1997 ; Short Protocols in Molecular Biology , John Wiley & Sons, New York, N.Y., unit 7.7) and in Meyers (1995 ; Molecular Biology and Biotechnology , Wiley VCH, New York, N.Y., p 856–853).
A further method for identifying or confirming that specific homologous sequences control the same function is by comparison of the transcript profile(s) obtained upon overexpression or knockout of two or more related transcription factors. Since transcript profiles are diagnostic for specific cellular states, one skilled in the art will appreciate that genes that have a highly similar transcript profile (e.g., with greater than 50% regulated transcripts in common, more preferably with greater than 70% regulated transcripts in common, most preferably with greater than 90% regulated transcripts in common) will have highly similar functions. Fowler et al. (2002) Plant Cell 14: 1675–79) have shown that three paralogous AP2 family genes (CBF1, CBF2 and CBF3), each of which is induced upon cold treatment, and each of which can condition improved freezing tolerance, have highly similar transcript profiles. Once a transcription factor has been shown to provide a specific function, its transcript profile becomes a diagnostic tool to determine whether putative paralogs or orthologs have the same function.
Furthermore, methods using manual alignment of sequences similar or homologous to one or more polynucleotide sequences or one or more polypeptides encoded by the polynucleotide sequences may be used to identify regions of similarity and conserved domains. Such manual methods are well-known of those of skill in the art and can include, for example, comparisons of tertiary structure between a polypeptide sequence encoded by a polynucleotide which comprises a known function with a polypeptide sequence encoded by a polynucleotide sequence which has a function not yet determined. Such examples of tertiary structure may comprise predicted alpha helices, beta-sheets, amphipathic helices, leucine zipper motifs, zinc finger motifs, proline-rich regions, cysteine repeat motifs, and the like.
Orthologs and paralogs of presently disclosed transcription factors may be cloned using compositions provided by the present invention according to methods well known in the art. cDNAs can be cloned using mRNA from a plant cell or tissue that expresses one of the present transcription factors. Appropriate mRNA sources may be identified by interrogating Northern blots with probes designed from the present transcription factor sequences, after which a library is prepared from the mRNA obtained from a positive cell or tissue. Transcription factor-encoding cDNA is then isolated using, for example, PCR, using primers designed from a presently disclosed transcription factor gene sequence, or by probing with a partial or complete cDNA or with one or more sets of degenerate probes based on the disclosed sequences. The cDNA library may be used to transform plant cells. Expression of the cDNAs of interest is detected using, for example, methods disclosed herein such as microarrays, Northern blots, quantitative PCR, or any other technique for monitoring changes in expression. Genomic clones may be isolated using similar techniques to those.
Identifying Polynucleotides or Nucleic Acids by Hybridization
Polynucleotides homologous to the sequences illustrated in the Sequence Listing and tables can be identified, e.g., by hybridization to each other under stringent or under highly stringent conditions. Single stranded polynucleotides hybridize when they associate based on a variety of well characterized physical-chemical forces, such as hydrogen bonding, solvent exclusion, base stacking and the like. The stringency of a hybridization reflects the degree of sequence identity of the nucleic acids involved, such that the higher the stringency, the more similar are the two polynucleotide strands. Stringency is influenced by a variety of factors, including temperature, salt concentration and composition, organic and non-organic additives, solvents, etc. present in both the hybridization and wash solutions and incubations (and number thereof), as described in more detail in the references cited above.
Encompassed by the invention are polynucleotide sequences that are capable of hybridizing to the claimed polynucleotide sequences, including any of the transcription factor polynucleotides within the Sequence Listing, and fragments thereof under various conditions of stringency (See, for example, Wahl and Berger (1987) Methods Enzymol. 152: 399–407; and Kimmel (1987) Methods Enzymol. 152: 507–511). In addition to the nucleotide sequences listed in Tables 4–9, full length cDNA, orthologs, and paralogs of the present nucleotide sequences may be identified and isolated using well-known methods. The cDNA libraries, orthologs, and paralogs of the present nucleotide sequences may be screened using hybridization methods to determine their utility as hybridization target or amplification probes.
With regard to hybridization, conditions that are highly stringent, and means for achieving them, are well known in the art. See, for example, Sambrook et al. (1989) “ Molecular Cloning: A Laboratory Manual ” (2nd ed., Cold Spring Harbor Laboratory); Berger and Kimmel, eds., (1987) “Guide to Molecular Cloning Techniques”, In Methods in Enzymology: 152: 467–469; and Anderson and Young (1985) “Quantitative Filter Hybridisation.” In: Hames and Higgins, ed., Nucleic Acid Hybridisation, A Practical Approach . Oxford, IRL Press, 73–111.
Stability of DNA duplexes is affected by such factors as base composition, length, and degree of base pair mismatch. Hybridization conditions may be adjusted to allow DNAs of different sequence relatedness to hybridize. The melting temperature (T m ) is defined as the temperature when 50% of the duplex molecules have dissociated into their constituent single strands. The melting temperature of a perfectly matched duplex, where the hybridization buffer contains formamide as a denaturing agent, may be estimated by the following equations:

T m (° C.)=81.5+16.6(log [Na+])+0.41(% G+C )−0.62(% formamide)−500/L (I) DNA-DNA:

T m (° C.)=79.8+18.5(log [Na+])+0.58(% G+C )+0.12(% G+C ) 2 −0.5(% formamide)−820/L (II) DNA-RNA:

T m (° C.)=79.8+18.5(log [Na+])+0.58(% G+C )+0.12(% G+C) 2 −0.35(% formamide)−820/L (III) RNA-RNA:

where L is the length of the duplex formed, [Na+] is the molar concentration of the sodium ion in the hybridization or washing solution, and % G+C is the percentage of (guanine+cytosine) bases in the hybrid. For imperfectly matched hybrids, approximately 1° C. is required to reduce the melting temperature for each 1% mismatch.
Hybridization experiments are generally conducted in a buffer of pH between 6.8 to 7.4, although the rate of hybridization is nearly independent of pH at ionic strengths likely to be used in the hybridization buffer (Anderson et al. (1985) supra). In addition, one or more of the following may be used to reduce non-specific hybridization: sonicated salmon sperm DNA or another non-complementary DNA, bovine serum albumin, sodium pyrophosphate, sodium dodecylsulfate (SDS), polyvinyl-pyrrolidone, ficoll and Denhardt's solution. Dextran sulfate and polyethylene glycol 6000 act to exclude DNA from solution, thus raising the effective probe DNA concentration and the hybridization signal within a given unit of time. In some instances, conditions of even greater stringency may be desirable or required to reduce non-specific and/or background hybridization. These conditions may be created with the use of higher temperature, lower ionic strength and higher concentration of a denaturing agent such as formamide.
Stringency conditions can be adjusted to screen for moderately similar fragments such as homologous sequences from distantly related organisms, or to highly similar fragments such as genes that duplicate functional enzymes from closely related organisms. The stringency can be adjusted either during the hybridization step or in the post-hybridization washes. Salt concentration, formamide concentration, hybridization temperature and probe lengths are variables that can be used to alter stringency (as described by the formula above). As a general guidelines high stringency is typically performed at T m −5° C. to T m −20° C., moderate stringency at T m −20° C. to T m −35° C. and low stringency at T m −35° C. to T m −50° C. for duplex>150 base pairs. Hybridization may be performed at low to moderate stringency (25–50° C. below T m ), followed by post-hybridization washes at increasing stringencies. Maximum rates of hybridization in solution are determined empirically to occur at T m −25° C. for DNA-DNA duplex and T m −15° C. for RNA-DNA duplex. Optionally, the degree of dissociation may be assessed after each wash step to determine the need for subsequent, higher stringency wash steps.
High stringency conditions may be used to select for nucleic acid sequences with high degrees of identity to the disclosed sequences. An example of stringent hybridization conditions obtained in a filter-based method such as a Southern or northern blot for hybridization of complementary nucleic acids that have more than 100 complementary residues is about 5° C. to 20° C. lower than the thermal melting point (T m ) for the specific sequence at a defined ionic strength and pH. Conditions used for hybridization may include about 0.02 M to about 0.15 M sodium chloride, about 0.5% to about 5% casein, about 0.02% SDS or about 0.1% N-laurylsarcosine, about 0.001 M to about 0.03 M sodium citrate, at hybridization temperatures between about 50° C. and about 70° C. More preferably, high stringency conditions are about 0.02 M sodium chloride, about 0.5% casein, about 0.02% SDS, about 0.001 M sodium citrate, at a temperature of about 50° C. Nucleic acid molecules that hybridize under stringent conditions will typically hybridize to a probe based on either the entire DNA molecule or selected portions, e.g., to a unique subsequence, of the DNA.
Stringent salt concentration will ordinarily be less than about 750 mM NaCl and 75 mM trisodium citrate. Increasingly stringent conditions may be obtained with less than about 500 mM NaCl and 50 mM trisodium citrate, to even greater stringency with less than about 250 mM NaCl and 25 mM trisodium citrate. Low stringency hybridization can be obtained in the absence of organic solvent, e.g., formamide, whereas high stringency hybridization may be obtained in the presence of at least about 35% formamide, and more preferably at least about 50% formamide. Stringent temperature conditions will ordinarily include temperatures of at least about 30° C., more preferably of at least about 37° C., and most preferably of at least about 42° C. with formamide present. Varying additional parameters, such as hybridization time, the concentration of detergent, e.g., sodium dodecyl sulfate (SDS) and ionic strength, are well known to those skilled in the art. Various levels of stringency are accomplished by combining these various conditions as needed.
The washing steps that follow hybridization may also vary in stringency; the post-hybridization wash steps primarily determine hybridization specificity, with the most critical factors being temperature and the ionic strength of the final wash solution. Wash stringency can be increased by decreasing salt concentration or by increasing temperature. Stringent salt concentration for the wash steps will preferably be less than about 30 mM NaCl and 3 mM trisodium citrate, and most preferably less than about 15 mM NaCl and 1.5 mM trisodium citrate.
Thus, hybridization and wash conditions that may be used to bind and remove polynucleotides with less than the desired homology to the nucleic acid sequences or their complements that encode the present transcription factors include, for example:
6×SSC at 65° C.;
50% formamide, 4×SSC at 42° C.; or
0.5×SSC, 0.1% SDS at 65° C.;
with, for example, two wash steps of 10–30 minutes each. Useful variations on these conditions will be readily apparent to those skilled in the art.
A person of skill in the art would not expect substantial variation among polynucleotide species encompassed within the scope of the present invention because the highly stringent conditions set forth in the above formulae yield structurally similar polynucleotides.
If desired, one may employ wash steps of even greater stringency, including about 0.2×SSC, 0.1% SDS at 65° C. and washing twice, each wash step being about 30 min, or about 0.1×SSC, 0.1% SDS at 65° C. and washing twice for 30 min. The temperature for the wash solutions will ordinarily be at least about 25° C., and for greater stringency at least about 42° C. Hybridization stringency may be increased further by using the same conditions as in the hybridization steps, with the wash temperature raised about 3° C. to about 5° C., and stringency may be increased even further by using the same conditions except the wash temperature is raised about 6° C. to about 9° C. For identification of less closely related homologs, wash steps may be performed at a lower temperature, e.g., 50° C.
An example of a low stringency wash step employs a solution and conditions of at least 25° C. in 30 mM NaCl, 3 mM trisodium citrate, and 0.1% SDS over 30 min. Greater stringency may be obtained at 42° C. in 15 mM NaCl, with 1.5 mM trisodium citrate, and 0.1% SDS over 30 min. Even higher stringency wash conditions are obtained at 65° C.–68° C. in a solution of 15 mM NaCl, 1.5 mM trisodium citrate, and 0.1% SDS. Wash procedures will generally employ at least two final wash steps. Additional variations on these conditions will be readily apparent to those skilled in the art (see, for example, US Patent Application No. 20010010913).
Stringency conditions can be selected such that an oligonucleotide that is perfectly complementary to the coding oligonucleotide hybridizes to the coding oligonucleotide with at least about a 5–10× higher signal to noise ratio than the ratio for hybridization of the perfectly complementary oligonucleotide to a nucleic acid encoding a transcription factor known as of the filing date of the application. It may be desirable to select conditions for a particular assay such that a higher signal to noise ratio, that is, about 15× or more, is obtained. Accordingly, a subject nucleic acid will hybridize to a unique coding oligonucleotide with at least a 2× or greater signal to noise ratio as compared to hybridization of the coding oligonucleotide to a nucleic acid encoding known polypeptide. The particular signal will depend on the label used in the relevant assay, e.g., a fluorescent label, a calorimetric label, a radioactive label, or the like. Labeled hybridization or PCR probes for detecting related polynucleotide sequences may be produced by oligolabeling, nick translation, end-labeling, or PCR amplification using a labeled nucleotide.
Encompassed by the invention are polynucleotide sequences encoding polypeptides capable of regulating transcription, said polynucleotide sequences being capable of hybridizing to the claimed polynucleotide sequences, including those listed in the Sequence Listing, or polynucleotides that encode the polypeptides listed in the Sequence Listing, and specifically SEQ ID NOs: 1–2237, and fragments thereof under various conditions of stringency. (See, e.g., Wahl and Berger (1987) Methods Enzymol. 152: 399–407; Kimmel (1987) Methods Enzymol. 152: 507–511.) Estimates of homology are provided by either DNA-DNA or DNA-RNA hybridization under conditions of stringency as is well understood by those skilled in the art (Hames and Higgins, Eds. (1985) Nucleic Acid Hybridisation , IRL Press, Oxford, U.K.). Stringency conditions can be adjusted to screen for moderately similar fragments, such as homologous sequences from distantly related organisms, to highly similar fragments, such as genes that duplicate functional enzymes from closely related organisms. Post-hybridization washes determine stringency conditions.
Identifying Polynucleotides or Nucleic Acids with Expression Libraries
In addition to hybridization methods, transcription factor homolog polypeptides can be obtained by screening an expression library using antibodies specific for one or more transcription factors. With the provision herein of the disclosed transcription factor, and transcription factor homolog nucleic acid sequences, the encoded polypeptide(s) can be expressed and purified in a heterologous expression system (e.g., E. coli ) and used to raise antibodies (monoclonal or polyclonal) specific for the polypeptide(s) in question. Antibodies can also be raised against synthetic peptides derived from transcription factor, or transcription factor homolog, amino acid sequences. Methods of raising antibodies are well known in the art and are described in Harlow and Lane (1988), Antibodies: A Laboratory Manual , Cold Spring Harbor Laboratory, New York. Such antibodies can then be used to screen an expression library produced from the plant from which it is desired to clone additional transcription factor homologs, using the methods described above. The selected cDNAs can be confirmed by sequencing and enzymatic activity.
Sequence Variations
It will readily be appreciated by those of skill in the art, that any of a variety of polynucleotide sequences are capable of encoding the transcription factors and transcription factor homolog polypeptides of the invention. Due to the degeneracy of the genetic code, many different polynucleotides can encode identical and/or substantially similar polypeptides in addition to those sequences illustrated in the Sequence Listing. Nucleic acids having a sequence that differs from the sequences shown in the Sequence Listing, or complementary sequences, that encode functionally equivalent peptides (i.e., peptides having some degree of equivalent or similar biological activity) but differ in sequence from the sequence shown in the Sequence Listing due to degeneracy in the genetic code, are also within the scope of the invention.
Altered polynucleotide sequences encoding polypeptides include those sequences with deletions, insertions, or substitutions of different nucleotides, resulting in a polynucleotide encoding a polypeptide with at least one functional characteristic of the instant polypeptides. Included within this definition are polymorphisms which may or may not be readily detectable using a particular oligonucleotide probe of the polynucleotide encoding the instant polypeptides, and improper or unexpected hybridization to allelic variants, with a locus other than the normal chromosomal locus for the polynucleotide sequence encoding the instant polypeptides.
Allelic variant refers to any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymorphism within populations. Gene mutations can be silent (i.e., no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequence. The term allelic variant is also used herein to denote a protein encoded by an allelic variant of a gene. Splice variant refers to alternative forms of RNA transcribed from a gene. Splice variation arises naturally through use of alternative splicing sites within a transcribed RNA molecule, or less commonly between separately transcribed RNA molecules, and may result in several mRNAs transcribed from the same gene. Splice variants may encode polypeptides having altered amino acid sequence. The term splice variant is also used herein to denote a protein encoded by a splice variant of an mRNA transcribed from a gene.
Those skilled in the art would recognize that, for example, G47, SEQ ID NO: 12, represents a single transcription factor; allelic variation and alternative splicing may be expected to occur. Allelic variants of SEQ ID NO: 11 can be cloned by probing cDNA or genomic libraries from different individual organisms according to standard procedures. Allelic variants of the DNA sequence shown in SEQ ID NO: 11, including those containing silent mutations and those in which mutations result in amino acid sequence changes, are within the scope of the present invention, as are proteins which are allelic variants of SEQ ID NO: 12. cDNAs generated from alternatively spliced mRNAs, which retain the properties of the transcription factor are included within the scope of the present invention, as are polypeptides encoded by such cDNAs and mRNAs. Allelic variants and splice variants of these sequences can be cloned by probing cDNA or genomic libraries from different individual organisms or tissues according to standard procedures known in the art (see U.S. Pat. No. 6,388,064).
Thus, in addition to the sequences set forth in the Sequence Listing, the invention also encompasses related nucleic acid molecules that include allelic or splice variants of SEQ ID NO: 2N−1, where N=1–335, sequences that are orthologous to SEQ ID NOs: 761–1348, 1557–2101, and 2124–2237), sequences that are orthologous to paralogous to SEQ ID NOs: 1349–1556, variant sequences that have been shown to confer an altered trait listed in Table 4 (SEQ ID NOs: 2102–2123) listed in the Sequence Listing, and sequences that are complementary to any of the above nucleotide sequences. Related nucleic acid molecules also include nucleotide sequences encoding a polypeptide comprising or consisting essentially of a substitution, modification, addition and/or deletion of one or more amino acid residues compared to the polypeptides as set forth in the Sequence Listing. Such related polypeptides may comprise, for example, additions and/or deletions of one or more N-linked or O-linked glycosylation sites, or an addition and/or a deletion of one or more cysteine residues.
For example, Table 1 illustrates, e.g., that the codons AGC, AGT, TCA, TCC, TCG, and TCT all encode the same amino acid: serine. Accordingly, at each position in the sequence where there is a codon encoding serine, any of the above trinucleotide sequences can be used without altering the encoded polypeptide.






TABLE 1



Amino acid
Possible Codons















Alanine
Ala
A
GCA
GCG
GCG
GCU



Cysteine
Cys
C
TGC
TGT

Aspartic acid
Asp
D
GAC
GAT

Glutamic acid
Glu
E
GAA
GAG

Phenylalanine
Phe
F
TTC
TTT

Glycine
Gly
G
GGA
GGC
GGG
GGT

Histidine
His
H
CAC
CAT

Isoleucine
Ile
I
ATA
ATC
ATT

Lysine
Lys
K
AAA
AAG

Leucine
Leu
L
TTA
TTG
CTA
CTC
CTG
CTT

Methionine
Met
M
ATG

Asparagine
Asn
N
AAC
AAT

Proline
Pro
P
CCA
CCC
CCG
CCT

Glutamine
Gln
Q
CAA
CAG

Arginine
Arg
R
AGA
AGG
CGA
CGC
CGG
CGT

Serine
Ser
S
AGC
AGT
TCA
TCC
TCG
TCT

Threonine
Thr
T
ACA
ACC
ACG
ACT

Valine
Val
V
GTA
GTC
GTG
GTT

Tryptophan
Trp
W
TGG

Tyrosine
Tyr
Y
TAC
TAT








































Sequence alterations that do not change the amino acid sequence encoded by the polynucleotide are termed “silent” variations. With the exception of the codons ATG and TGG, encoding methionine and tryptophan, respectively, any of the possible codons for the same amino acid can be substituted by a variety of techniques, e.g., site-directed mutagenesis, available in the art. Accordingly, any and all such variations of a sequence selected from the above table are a feature of the invention.
In addition to silent variations, other conservative variations that alter one, or a few amino acids in the encoded polypeptide, can be made without altering the function of the polypeptide, these conservative variants are, likewise, a feature of the invention.
For example, substitutions, deletions and insertions introduced into the sequences provided in the Sequence Listing, are also envisioned by the invention. Such sequence modifications can be engineered into a sequence by site-directed mutagenesis (Wu (ed.) Methods Enzymol . (1993) vol. 217, Academic Press) or the other methods noted below. Amino acid substitutions are typically of single residues; insertions usually will be on the order of about from 1 to 10 amino acid residues; and deletions will range about from 1 to 30 residues. In preferred embodiments, deletions or insertions are made in adjacent pairs, e.g., a deletion of two residues or insertion of two residues. Substitutions, deletions, insertions or any combination thereof can be combined to arrive at a sequence. The mutations that are made in the polynucleotide encoding the transcription factor should not place the sequence out of reading frame and should not create complementary regions that could produce secondary mRNA structure. Preferably, the polypeptide encoded by the DNA performs the desired function.
Conservative substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 2 when it is desired to maintain the activity of the protein. Table 2 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as conservative substitutions.








TABLE 2






Conservative


Residue
Substitutions





Ala
Ser


Arg
Lys


Asn
Gln; His


Asp
Glu


Gln
Asn


Cys
Ser


Glu
Asp


Gly
Pro


His
Asn; Gln


Ile
Leu, Val


Leu
Ile; Val


Lys
Arg; Gln


Met
Leu; Ile


Phe
Met; Leu; Tyr


Ser
Thr; Gly


Thr
Ser; Val


Trp
Tyr


Tyr
Trp; Phe


Val
Ile; Leu





































Similar substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 3 when it is desired to maintain the activity of the protein. Table 3 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as structural and functional substitutions. For example, a residue in column 1 of Table 3 may be substituted with a residue in column 2; in addition, a residue in column 2 of Table 3 may be substituted with the residue of column 1.








TABLE 3





Residue
Similar Substitutions





Ala
Ser; Thr; Gly; Val; Leu; Ile


Arg
Lys; His; Gly


Asn
Gln; His; Gly; Ser; Thr


Asp
Glu, Ser; Thr


Gln
Asn; Ala


Cys
Ser; Gly


Glu
Asp


Gly
Pro; Arg


His
Asn; Gln; Tyr; Phe; Lys; Arg


Ile
Ala; Leu; Val; Gly; Met


Leu
Ala; Ile; Val; Gly; Met


Lys
Arg; His; Gln; Gly; Pro


Met
Leu; Ile; Phe


Phe
Met; Leu; Tyr; Trp; His; Val; Ala


Ser
Thr; Gly; Asp; Ala; Val; Ile; His


Thr
Ser; Val; Ala; Gly


Trp
Tyr; Phe; His


Tyr
Trp; Phe; His


Val
Ala; Ile; Leu; Gly; Thr; Ser; Glu




































Substitutions that are less conservative than those in Table 2 can be selected by picking residues that differ more significantly in their effect on maintaining (a) the structure of the polypeptide backbone in the area of the substitution, for example, as a sheet or helical conformation, (b) the charge or hydrophobicity of the molecule at the target site, or (c) the bulk of the side chain. The substitutions which in general are expected to produce the greatest changes in protein properties will be those in which (a) a hydrophilic residue, e.g., seryl or threonyl, is substituted for (or by) a hydrophobic residue, e.g., leucyl, isoleucyl, phenylalanyl, valyl or alanyl; (b) a cysteine or proline is substituted for (or by) any other residue; (c) a residue having an electropositive side chain, e.g., lysyl, arginyl, or histidyl, is substituted for (or by) an electronegative residue, e.g., glutamyl or aspartyl; or (d) a residue having a bulky side chain, e.g., phenylalanine, is substituted for (or by) one not having a side chain, e.g., glycine.
Further Modifying Sequences of the Invention—Mutation/Forced Evolution
In addition to generating silent or conservative substitutions as noted, above, the present invention optionally includes methods of modifying the sequences of the Sequence Listing. In the methods, nucleic acid or protein modification methods are used to alter the given sequences to produce new sequences and/or to chemically or enzymatically modify given sequences to change the properties of the nucleic acids or proteins.
Thus, in one embodiment, given nucleic acid sequences are modified, e.g., according to standard mutagenesis or artificial evolution methods to produce modified sequences. The modified sequences may be created using purified natural polynucleotides isolated from any organism or may be synthesized from purified compositions and chemicals using chemical means well know to those of skill in the art. For example, Ausubel, supra, provides additional details on mutagenesis methods. Artificial forced evolution methods are described, for example, by Stemmer (1994) Nature 370: 389–391, Stemmer (1994) Proc. Natl. Acad. Sci. 91: 10747–10751, and U.S. Pat. Nos. 5,811,238, 5,837,500, and 6,242,568. Methods for engineering synthetic transcription factors and other polypeptides are described, for example, by Zhang et al. (2000) J. Biol. Chem. 275: 33850–33860, Liu et al. (2001) J. Biol. Chem. 276: 11323–11334, and Isalan et al. (2001) Nature Biotechnol. 19: 656–660. Many other mutation and evolution methods are also available and expected to be within the skill of the practitioner.
Similarly, chemical or enzymatic alteration of expressed nucleic acids and polypeptides can be performed by standard methods. For example, sequence can be modified by addition of lipids, sugars, peptides, organic or inorganic compounds, by the inclusion of modified nucleotides or amino acids, or the like. For example, protein modification techniques are illustrated in Ausubel, supra. Further details on chemical and enzymatic modifications can be found herein. These modification methods can be used to modify any given sequence, or to modify any sequence produced by the various mutation and artificial evolution modification methods noted herein.
Accordingly, the invention provides for modification of any given nucleic acid by mutation, evolution, chemical or enzymatic modification, or other available methods, as well as for the products produced by practicing such methods, e.g., using the sequences herein as a starting substrate for the various modification approaches.
For example, optimized coding sequence containing codons preferred by a particular prokaryotic or eukaryotic host can be used e.g., to increase the rate of translation or to produce recombinant RNA transcripts having desirable properties, such as a longer half-life, as compared with transcripts produced using a non-optimized sequence. Translation stop codons can also be modified to reflect host preference. For example, preferred stop codons for Saccharomyces cerevisiae and mammals are TAA and TGA, respectively. The preferred stop codon for monocotyledonous plants is TGA, whereas insects and E. coli prefer to use TAA as the stop codon.
The polynucleotide sequences of the present invention can also be engineered in order to alter a coding sequence for a variety of reasons, including but not limited to, alterations which modify the sequence to facilitate cloning, processing and/or expression of the gene product. For example, alterations are optionally introduced using techniques which are well known in the art, e.g., site-directed mutagenesis, to insert new restriction sites, to alter glycosylation patterns, to change codon preference, to introduce splice sites, etc.
Furthermore, a fragment or domain derived from any of the polypeptides of the invention can be combined with domains derived from other transcription factors or synthetic domains to modify the biological activity of a transcription factor. For instance, a DNA-binding domain derived from a transcription factor of the invention can be combined with the activation domain of another transcription factor or with a synthetic activation domain. A transcription activation domain assists in initiating transcription from a DNA-binding site. Examples include the transcription activation region of VP16 or GAL4 (Moore et al. (1998) Proc. Natl. Acad. Sci. 95: 376–381; Aoyama et al. (1995) Plant Cell 7: 1773–1785), peptides derived from bacterial sequences (Ma and Ptashne (1987) Cell 51: 113–119) and synthetic peptides (Giniger and Ptashne (1987) Nature 330: 670–672).
Expression and Modification of Polypeptides
Typically, polynucleotide sequences of the invention are incorporated into recombinant DNA (or RNA) molecules that direct expression of polypeptides of the invention in appropriate host cells, transgenic plants, in vitro translation systems, or the like. Due to the inherent degeneracy of the genetic code, nucleic acid sequences which encode substantially the same or a functionally equivalent amino acid sequence can be substituted for any listed sequence to provide for cloning and expressing the relevant homolog.
The transgenic plants of the present invention comprising recombinant polynucleotide sequences are generally derived from parental plants, which may themselves be non-transformed (or non-transgenic) plants. These transgenic plants may either have a transcription factor gene “knocked out” (for example, with a genomic insertion by homologous recombination, an antisense or ribozyme construct) or expressed to a normal or wild-type extent. However, overexpressing transgenic “progeny” plants will exhibit greater mRNA levels, wherein the mRNA encodes a transcription factor, that is, a DNA-binding protein that is capable of binding to a DNA regulatory sequence and inducing transcription, and preferably, expression of a plant trait gene. Preferably, the mRNA expression level will be at least three-fold greater than that of the parental plant, or more preferably at least ten-fold greater mRNA levels compared to said parental plant, and most preferably at least fifty-fold greater compared to said parental plant.
Vectors, Promoters, and Expression Systems
The present invention includes recombinant constructs comprising one or more of the nucleic acid sequences herein. The constructs typically comprise a vector, such as a plasmid, a cosmid, a phage, a virus (e.g., a plant virus), a bacterial artificial chromosome (BAC), a yeast artificial chromosome (YAC), or the like, into which a nucleic acid sequence of the invention has been inserted, in a forward or reverse orientation. In a preferred aspect of this embodiment, the construct further comprises regulatory sequences, including, for example, a promoter, operably linked to the sequence. Large numbers of suitable vectors and promoters are known to those of skill in the art, and are commercially available.
General texts that describe molecular biological techniques useful herein, including the use and production of vectors, promoters and many other relevant topics, include Berger, Sambrook, supra and Ausubel, supra. Any of the identified sequences can be incorporated into a cassette or vector, e.g., for expression in plants. A number of expression vectors suitable for stable transformation of plant cells or for the establishment of transgenic plants have been described including those described in Weissbach and Weissbach (1989) Methods for Plant Molecular Biology , Academic Press, and Gelvin et al. (1990) Plant Molecular Biology Manual , Kluwer Academic Publishers. Specific examples include those derived from a Ti plasmid of Agrobacterium tumefaciens , as well as those disclosed by Herrera-Estrella et al. (1983) Nature 303: 209, Bevan (1984) Nucleic Acids Res. 12: 8711–8721, Klee (1985) Bio/Technology 3: 637–642, for dicotyledonous plants.
Alternatively, non-Ti vectors can be used to transfer the DNA into monocotyledonous plants and cells by using free DNA delivery techniques. Such methods can involve, for example, the use of liposomes, electroporation, microprojectile bombardment, silicon carbide whiskers, and viruses. By using these methods transgenic plants such as wheat, rice (Christou (1991) Bio/Technology 9: 957–962) and corn (Gordon-Kamm (1990) Plant Cell 2: 603–618) can be produced. An immature embryo can also be a good target tissue for monocots for direct DNA delivery techniques by using the particle gun (Weeks et al. (1993) Plant Physiol. 102: 1077–1084; Vasil (1993) Bio/Technology 10: 667–674; Wan and Lemeaux (1994) Plant Physiol. 104: 3748, and for Agrobacterium -mediated DNA transfer (Ishida et al. (1996) Nature Biotechnol. 14: 745–750).
Typically, plant transformation vectors include one or more cloned plant coding sequence (genomic or cDNA) under the transcriptional control of 5′ and 3′ regulatory sequences and a dominant selectable marker. Such plant transformation vectors typically also contain a promoter (e.g., a regulatory region controlling inducible or constitutive, environmentally- or developmentally-regulated, or cell- or tissue-specific expression), a transcription initiation start site, an RNA processing signal (such as intron splice sites), a transcription termination site, and/or a polyadenylation signal.
A potential utility for the transcription factor polynucleotides disclosed herein is the isolation of promoter elements from these genes that can be used to program expression in plants of any genes. Each transcription factor gene disclosed herein is expressed in a unique fashion, as determined by promoter elements located upstream of the start of translation, and additionally within an intron of the transcription factor gene or downstream of the termination codon of the gene. As is well known in the art, for a significant portion of genes, the promoter sequences are located entirely in the region directly upstream of the start of translation. In such cases, typically the promoter sequences are located within 2.0 kb of the start of translation, or within 1.5 kb of the start of translation, frequently within 1.0 kb of the start of translation, and sometimes within 0.5 kb of the start of translation.
The promoter sequences can be isolated according to methods known to one skilled in the art.
Examples of constitutive plant promoters which can be useful for expressing the TF sequence include: the cauliflower mosaic virus (CaMV) 35S promoter, which confers constitutive, high-level expression in most plant tissues (see, e.g., Odell et al. (1985) Nature 313: 810–812); the nopaline synthase promoter (An et al. (1988) Plant Physiol. 88: 547–552); and the octopine synthase promoter (Fromm et al. (1989) Plant Cell 1: 977–984).
A variety of plant gene promoters that regulate gene expression in response to environmental, hormonal, chemical, developmental signals, and in a tissue-active manner can be used for expression of a TF sequence in plants. Choice of a promoter is based largely on the phenotype of interest and is determined by such factors as tissue (e.g., seed, fruit, root, pollen, vascular tissue, flower, carpel, etc.), inducibility (e.g., in response to wounding, heat, cold, drought, light, pathogens, etc.), timing, developmental stage, and the like. Numerous known promoters have been characterized and can favorably be employed to promote expression of a polynucleotide of the invention in a transgenic plant or cell of interest. For example, tissue specific promoters include: seed-specific promoters (such as the napin, phaseolin or DC3 promoter described in U.S. Pat. No. 5,773,697), fruit-specific promoters that are active during fruit ripening (such as the dru 1 promoter (U.S. Pat. No. 5,783,393), or the 2A11 promoter (U.S. Pat. No. 4,943,674) and the tomato polygalacturonase promoter (Bird et al. (1988) Plant Mol. Biol. 11: 651–662), root-specific promoters, such as those disclosed in U.S. Pat. Nos. 5,618,988, 5,837,848 and 5,905,186, pollen-active promoters such as PTA29, PTA26 and PTA13 (U.S. Pat. No. 5,792,929), promoters active in vascular tissue (Ringli and Keller (1998) Plant Mol. Biol. 37: 977–988), flower-specific (Kaiser et al. (1995) Plant Mol. Biol. 28: 231–243), pollen (Baerson et al. (1994) Plant Mol. Biol. 26: 1947–1959), carpels (Ohl et al. (1990) Plant Cell 2: 837–848), pollen and ovules (Baerson et al. (1993) Plant Mol. Biol. 22: 255–267), auxin-inducible promoters (such as that described in van der Kop et al. (1999) Plant Mol. Biol. 39: 979–990 or Baumann et al., (1999) Plant Cell 11: 323–334), cytokinin-inducible promoter (Guevara-Garcia (1998) Plant Mol. Biol. 38: 743–753), promoters responsive to gibberellin (Shi et al. (1998) Plant Mol. Biol. 38: 1053–1060, Willmott et al. (1998) Plant Molec. Biol. 38: 817–825) and the like. Additional promoters are those that elicit expression in response to heat (Ainley et al. (1993) Plant Mol. Biol. 22: 13–23), light (e.g., the pea rbcS-3A promoter, Kuhlemeier et al. (1989) Plant Cell 1: 471–478, and the maize rbcS promoter, Schaffner and Sheen (1991) Plant Cell 3: 997–1012); wounding (e.g., wunI, Siebertz et al. (1989) Plant Cell 1: 961–968); pathogens (such as the PR-1 promoter described in Buchel et al. (1999) Plant Mol. Biol. 40: 387–396, and the PDF1.2 promoter described in Manners et al. (1998) Plant Mol. Biol. 38: 1071–1080), and chemicals such as methyl jasmonate or salicylic acid (Gatz (1997) Annu. Rev. Plant Physiol. Plant Mol. Biol. 48: 89–108). In addition, the timing of the expression can be controlled by using promoters such as those acting at senescence (Gan and Amasino (1995) Science 270: 1986–1988); or late seed development (Odell et al. (1994) Plant Physiol. 106: 447–458).
Plant expression vectors can also include RNA processing signals that can be positioned within, upstream or downstream of the coding sequence. In addition, the expression vectors can include additional regulatory sequences from the 3′-untranslated region of plant genes, e.g., a 3′ terminator region to increase mRNA stability of the mRNA, such as the PI-II terminator region of potato or the octopine or nopaline synthase 3′ terminator regions.
Additional Expression Elements
Specific initiation signals can aid in efficient translation of coding sequences. These signals can include, e.g., the ATG initiation codon and adjacent sequences. In cases where a coding sequence, its initiation codon and upstream sequences are inserted into the appropriate expression vector, no additional translational control signals may be needed. However, in cases where only coding sequence (e.g., a mature protein coding sequence), or a portion thereof, is inserted, exogenous transcriptional control signals including the ATG initiation codon can be separately provided. The initiation codon is provided in the correct reading frame to facilitate transcription. Exogenous transcriptional elements and initiation codons can be of various origins, both natural and synthetic. The efficiency of expression can be enhanced by the inclusion of enhancers appropriate to the cell system in use.
Expression Hosts
The present invention also relates to host cells which are transduced with vectors of the invention, and the production of polypeptides of the invention (including fragments thereof) by recombinant techniques. Host cells are genetically engineered (i.e., nucleic acids are introduced, e.g., transduced, transformed or transfected) with the vectors of this invention, which may be, for example, a cloning vector or an expression vector comprising the relevant nucleic acids herein. The vector is optionally a plasmid, a viral particle, a phage, a naked nucleic acid, etc. The engineered host cells can be cultured in conventional nutrient media modified as appropriate for activating promoters, selecting transformants, or amplifying the relevant gene. The culture conditions, such as temperature, pH and the like, are those previously used with the host cell selected for expression, and will be apparent to those skilled in the art and in the references cited herein, including, Sambrook, supra and Ausubel, supra.
The host cell can be a eukaryotic cell, such as a yeast cell, or a plant cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Plant protoplasts are also suitable for some applications. For example, the DNA fragments are introduced into plant tissues, cultured plant cells or plant protoplasts by standard methods including electroporation (Fromm et al. (1985) Proc. Natl. Acad. Sci. 82: 5824–5828, infection by viral vectors such as cauliflower mosaic virus (CaMV) (Hohn et al. (1982) Molecular Biology of Plant Tumors Academic Press, New York, N.Y., pp. 549–560; U.S. Pat. No. 4,407,956), high velocity ballistic penetration by small particles with the nucleic acid either within the matrix of small beads or particles, or on the surface (Klein et al. (1987) Nature 327: 70–73), use of pollen as vector (WO 85/01856), or use of Agrobacterium tumefaciens or A. rhizogenes carrying a T-DNA plasmid in which DNA fragments are cloned. The T-DNA plasmid is transmitted to plant cells upon infection by Agrobacterium tumefaciens , and a portion is stably integrated into the plant genome (Horsch et al. (1984) Science 233: 496–498; Fraley et al. (1983) Proc. Natl. Acad. Sci. 80: 4803–4807).
The cell can include a nucleic acid of the invention that encodes a polypeptide, wherein the cell expresses a polypeptide of the invention. The cell can also include vector sequences, or the like. Furthermore, cells and transgenic plants that include any polypeptide or nucleic acid above or throughout this specification, e.g., produced by transduction of a vector of the invention, are an additional feature of the invention.
For long-term, high-yield production of recombinant proteins, stable expression can be used. Host cells transformed with a nucleotide sequence encoding a polypeptide of the invention are optionally cultured under conditions suitable for the expression and recovery of the encoded protein from cell culture. The protein or fragment thereof produced by a recombinant cell may be secreted, membrane-bound, or contained intracellularly, depending on the sequence and/or the vector used. As will be understood by those of skill in the art, expression vectors containing polynucleotides encoding mature proteins of the invention can be designed with signal sequences which direct secretion of the mature polypeptides through a prokaryotic or eukaryotic cell membrane.
Modified Amino Acid Residues
Polypeptides of the invention may contain one or more modified amino acid residues. The presence of modified amino acids may be advantageous in, for example, increasing polypeptide half-life, reducing polypeptide antigenicity or toxicity, increasing polypeptide storage stability, or the like. Amino acid residue(s) are modified, for example, co-translationally or post-translationally during recombinant production or modified by synthetic or chemical means.
Non-limiting examples of a modified amino acid residue include incorporation or other use of acetylated amino acids, glycosylated amino acids, sulfated amino acids, prenylated (e.g., farnesylated, geranylgeranylated) amino acids, PEG modified (e.g., “PEGylated”) amino acids, biotinylated amino acids, carboxylated amino acids, phosphorylated amino acids, etc. References adequate to guide one of skill in the modification of amino acid residues are replete throughout the literature.
The modified amino acid residues may prevent or increase affinity of the polypeptide for another molecule, including, but not limited to, polynucleotide, proteins, carbohydrates, lipids and lipid derivatives, and other organic or synthetic compounds.
Identification of Additional Factors
A transcription factor provided by the present invention can also be used to identify additional endogenous or exogenous molecules that can affect a phenotype or trait of interest. On the one hand, such molecules include organic (small or large molecules) and/or inorganic compounds that affect expression of (i.e., regulate) a particular transcription factor. Alternatively, such molecules include endogenous molecules that are acted upon either at a transcriptional level by a transcription factor of the invention to modify a phenotype as desired. For example, the transcription factors can be employed to identify one or more downstream genes that are subject to a regulatory effect of the transcription factor. In one approach, a transcription factor or transcription factor homolog of the invention is expressed in a host cell, e.g., a transgenic plant cell, tissue or explant, and expression products, either RNA or protein, of likely or random targets are monitored, e.g., by hybridization to a microarray of nucleic acid probes corresponding to genes expressed in a tissue or cell type of interest, by two-dimensional gel electrophoresis of protein products, or by any other method known in the art for assessing expression of gene products at the level of RNA or protein. Alternatively, a transcription factor of the invention can be used to identify promoter sequences (such as binding sites on DNA sequences) involved in the regulation of a downstream target. After identifying a promoter sequence, interactions between the transcription factor and the promoter sequence can be modified by changing specific nucleotides in the promoter sequence or specific amino acids in the transcription factor that interact with the promoter sequence to alter a plant trait. Typically, transcription factor DNA-binding sites are identified by gel shift assays. After identifying the promoter regions, the promoter region sequences can be employed in double-stranded DNA arrays to identify molecules that affect the interactions of the transcription factors with their promoters (Bulyk et al. (1999) Nature Biotechnol. 17: 573–577).
The identified transcription factors are also useful to identify proteins that modify the activity of the transcription factor. Such modification can occur by covalent modification, such as by phosphorylation, or by protein—protein (homo or -heteropolymer) interactions. Any method suitable for detecting protein—protein interactions can be employed. Among the methods that can be employed are co-immunoprecipitation, cross-linking and co-purification through gradients or chromatographic columns, and the two-hybrid yeast system.
The two-hybrid system detects protein interactions in vivo and is described in Chien et al. ((1991) Proc. Natl. Acad. Sci. 88: 9578–9582) and is commercially available from Clontech (Palo Alto, Calif.). In such a system, plasmids are constructed that encode two hybrid proteins: one consists of the DNA-binding domain of a transcription activator protein fused to the TF polypeptide and the other consists of the transcription activator protein's activation domain fused to an unknown protein that is encoded by a cDNA that has been recombined into the plasmid as part of a cDNA library. The DNA-binding domain fusion plasmid and the cDNA library are transformed into a strain of the yeast Saccharomyces cerevisiae that contains a reporter gene (e.g., lacZ) whose regulatory region contains the transcription activator's binding site. Either hybrid protein alone cannot activate transcription of the reporter gene. Interaction of the two hybrid proteins reconstitutes the functional activator protein and results in expression of the reporter gene, which is detected by an assay for the reporter gene product. Then, the library plasmids responsible for reporter gene expression are isolated and sequenced to identify the proteins encoded by the library plasmids. After identifying proteins that interact with the transcription factors, assays for compounds that interfere with the TF protein—protein interactions can be preformed.
Identification of Modulators
In addition to the intracellular molecules described above, extracellular molecules that alter activity or expression of a transcription factor, either directly or indirectly, can be identified. For example, the methods can entail first placing a candidate molecule in contact with a plant or plant cell. The molecule can be introduced by topical administration, such as spraying or soaking of a plant, or incubating a plant in a solution containing the molecule, and then the molecule's effect on the expression or activity of the TF polypeptide or the expression of the polynucleotide monitored. Changes in the expression of the TF polypeptide can be monitored by use of polyclonal or monoclonal antibodies, gel electrophoresis or the like. Changes in the expression of the corresponding polynucleotide sequence can be detected by use of microarrays, Northerns, quantitative PCR, or any other technique for monitoring changes in mRNA expression. These techniques are exemplified in Ausubel et al. (eds.) Current Protocols in Molecular Biology , John Wiley & Sons (1998, and supplements through 2001). Changes in the activity of the transcription factor can be monitored, directly or indirectly, by assaying the function of the transcription factor, for example, by measuring the expression of promoters known to be controlled by the transcription factor (using promoter-reporter constructs), measuring the levels of transcripts using microarrays, Northern blots, quantitative PCR, etc. Such changes in the expression levels can be correlated with modified plant traits and thus identified molecules can be useful for soaking or spraying on fruit, vegetable and grain crops to modify traits in plants.
Essentially any available composition can be tested for modulatory activity of expression or activity of any nucleic acid or polypeptide herein. Thus, available libraries of compounds such as chemicals, polypeptides, nucleic acids and the like can be tested for modulatory activity. Often, potential modulator compounds can be dissolved in aqueous or organic (e.g., DMSO-based) solutions for easy delivery to the cell or plant of interest in which the activity of the modulator is to be tested. Optionally, the assays are designed to screen large modulator composition libraries by automating the assay steps and providing compounds from any convenient source to assays, which are typically run in parallel (e.g., in microtiter formats on micrometer plates in robotic assays).
In one embodiment, high throughput screening methods involve providing a combinatorial library containing a large number of potential compounds (potential modulator compounds). Such “combinatorial chemical libraries” are then screened in one or more assays, as described herein, to identify those library members (particular chemical species or subclasses) that display a desired characteristic activity. The compounds thus identified can serve as target compounds.
A combinatorial chemical library can be, e.g., a collection of diverse chemical compounds generated by chemical synthesis or biological synthesis. For example, a combinatorial chemical library such as a polypeptide library is formed by combining a set of chemical building blocks (e.g., in one example, amino acids) in every possible way for a given compound length (i.e., the number of amino acids in a polypeptide compound of a set length). Exemplary libraries include peptide libraries, nucleic acid libraries, antibody libraries (see, e.g., Vaughn et al. (1996) Nature Biotechnol. 14: 309–314 and PCT/US96/10287), carbohydrate libraries (see, e.g., Liang et al. Science (1996) 274: 1520–1522 and U.S. Pat. No. 5,593,853), peptide nucleic acid libraries (see, e.g., U.S. Pat. No. 5,539,083), and small organic molecule libraries (see, e.g., benzodiazepines, in Baum Chem . & Engineering News Jan. 18, 1993, page 33; isoprenoids, U.S. Pat. No. 5,569,588; thiazolidinones and metathiazanones, U.S. Pat. No. 5,549,974; pyrrolidines, U.S. Pat. Nos. 5,525,735 and 5,519,134; morpholino compounds, U.S. Pat. No. 5,506,337) and the like.
Preparation and screening of combinatorial or other libraries is well known to those of skill in the art. Such combinatorial chemical libraries include, but are not limited to, peptide libraries (see, e.g., U.S. Pat. No. 5,010,175; Furka, (1991) Int. J. Pept. Prot. Res. 37: 487–493; and Houghton et al. (1991) Nature 354: 84–88). Other chemistries for generating chemical diversity libraries can also be used.
In addition, as noted, compound screening equipment for high-throughput screening is generally available, e.g., using any of a number of well known robotic systems that have also been developed for solution phase chemistries useful in assay systems. These systems include automated workstations including an automated synthesis apparatus and robotic systems utilizing robotic arms. Any of the above devices are suitable for use with the present invention, e.g., for high-throughput screening of potential modulators. The nature and implementation of modifications to these devices (if any) so that they can operate as discussed herein will be apparent to persons skilled in the relevant art.
Indeed, entire high-throughput screening systems are commercially available. These systems typically automate entire procedures including all sample and reagent pipetting, liquid dispensing, timed incubations, and final readings of the microplate in detector(s) appropriate for the assay. These configurable systems provide high throughput and rapid start up as well as a high degree of flexibility and customization. Similarly, microfluidic implementations of screening are also commercially available.
The manufacturers of such systems provide detailed protocols the various high throughput. Thus, for example, Zymark Corp. provides technical bulletins describing screening systems for detecting the modulation of gene transcription, ligand binding, and the like. The integrated systems herein, in addition to providing for sequence alignment and, optionally, synthesis of relevant nucleic acids, can include such screening apparatus to identify modulators that have an effect on one or more polynucleotides or polypeptides according to the present invention.
In some assays it is desirable to have positive controls to ensure that the components of the assays are working properly. At least two types of positive controls are appropriate. That is, known transcriptional activators or inhibitors can be incubated with cells or plants, for example, in one sample of the assay, and the resulting increase/decrease in transcription can be detected by measuring the resulting increase in RNA levels and/or protein expression, for example, according to the methods herein. It will be appreciated that modulators can also be combined with transcriptional activators or inhibitors to find modulators that inhibit transcriptional activation or transcriptional repression. Either expression of the nucleic acids and proteins herein or any additional nucleic acids or proteins activated by the nucleic acids or proteins herein, or both, can be monitored.
In an embodiment, the invention provides a method for identifying compositions that modulate the activity or expression of a polynucleotide or polypeptide of the invention. For example, a test compound, whether a small or large molecule, is placed in contact with a cell, plant (or plant tissue or explant), or composition comprising the polynucleotide or polypeptide of interest and a resulting effect on the cell, plant, (or tissue or explant) or composition is evaluated by monitoring, either directly or indirectly, one or more of: expression level of the polynucleotide or polypeptide, activity (or modulation of the activity) of the polynucleotide or polypeptide. In some cases, an alteration in a plant phenotype can be detected following contact of a plant (or plant cell, or tissue or explant) with the putative modulator, e.g., by modulation of expression or activity of a polynucleotide or polypeptide of the invention. Modulation of expression or activity of a polynucleotide or polypeptide of the invention may also be caused by molecular elements in a signal transduction second messenger pathway and such modulation can affect similar elements in the same or another signal transduction second messenger pathway.
Subsequences
Also contemplated are uses of polynucleotides, also referred to herein as oligonucleotides, typically having at least 12 bases, preferably at least 15, more preferably at least 20, 30, or 50 bases, which hybridize under at least highly stringent (or ultra-high stringent or ultra-ultra-high stringent conditions) conditions to a polynucleotide sequence described above. The polynucleotides may be used as probes, primers, sense and antisense agents, and the like, according to methods as noted supra.
Subsequences of the polynucleotides of the invention, including polynucleotide fragments and oligonucleotides are useful as nucleic acid probes and primers. An oligonucleotide suitable for use as a probe or primer is at least about 15 nucleotides in length, more often at least about 18 nucleotides, often at least about 21 nucleotides, frequently at least about 30 nucleotides, or about 40 nucleotides, or more in length. A nucleic acid probe is useful in hybridization protocols, e.g., to identify additional polypeptide homologs of the invention, including protocols for microarray experiments. Primers can be annealed to a complementary target DNA strand by nucleic acid hybridization to form a hybrid between the primer and the target DNA strand, and then extended along the target DNA strand by a DNA polymerase enzyme. Primer pairs can be used for amplification of a nucleic acid sequence, e.g., by the polymerase chain reaction (PCR) or other nucleic-acid amplification methods. See Sambrook, supra, and Ausubel, supra.
In addition, the invention includes an isolated or recombinant polypeptide including a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotides of the invention. For example, such polypeptides, or domains or fragments thereof, can be used as immunogens, e.g., to produce antibodies specific for the polypeptide sequence, or as probes for detecting a sequence of interest. A subsequence can range in size from about 15 amino acids in length up to and including the full length of the polypeptide.
To be encompassed by the present invention, an expressed polypeptide which comprises such a polypeptide subsequence performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide. For example, a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA binding domain that activates transcription, e.g., by binding to a specific DNA promoter region an activation domain, or a domain for protein—protein interactions.
Production of Transgenic Plants
Modification of Traits
The polynucleotides of the invention are favorably employed to produce transgenic plants with various traits, or characteristics, that have been modified in a desirable manner, e.g., to improve the seed characteristics of a plant. For example, alteration of expression levels or patterns (e.g., spatial or temporal expression patterns) of one or more of the transcription factors (or transcription factor homologs) of the invention, as compared with the levels of the same protein found in a wild-type plant, can be used to modify a plant's traits. An illustrative example of trait modification, improved characteristics, by altering expression levels of a particular transcription factor is described further in the Examples and the Sequence Listing.
Arabidopsis as a Model System
Arabidopsis thaliana is the object of rapidly growing attention as a model for genetics and metabolism in plants. Arabidopsis has a small genome, and well-documented studies are available. It is easy to grow in large numbers and mutants defining important genetically controlled mechanisms are either available, or can readily be obtained. Various methods to introduce and express isolated homologous genes are available (see Koncz et al., eds., Methods in Arabidopsis Research (1992) World Scientific, New Jersey, N.J., in “Preface”). Because of its small size, short life cycle, obligate autogamy and high fertility, Arabidopsis is also a choice organism for the isolation of mutants and studies in morphogenetic and development pathways, and control of these pathways by transcription factors (Koncz supra, p. 72). A number of studies introducing transcription factors into A. thaliana have demonstrated the utility of this plant for understanding the mechanisms of gene regulation and trait alteration in plants. (See, for example, Koncz supra, and U.S. Pat. No. 6,417,428).
Homologous Genes Introduced into Transgenic Plants.
Homologous genes that may be derived from any plant, or from any source whether natural, synthetic, semi-synthetic or recombinant, and that share significant sequence identity or similarity to those provided by the present invention, may be introduced into plants, for example, crop plants, to confer desirable or improved traits. Consequently, transgenic plants may be produced that comprise a recombinant expression vector or cassette with a promoter operably linked to one or more sequences homologous to presently disclosed sequences. The promoter may be, for example, a plant or viral promoter.
The invention thus provides for methods for preparing transgenic plants, and for modifying plant traits. These methods include introducing into a plant a recombinant expression vector or cassette comprising a functional promoter operably linked to one or more sequences homologous to presently disclosed sequences. Plants and kits for producing these plants that result from the application of these methods are also encompassed by the present invention.
Transcription Factors of Interest for the Modification of Plant Traits
Currently, the existence of a series of maturity groups for different latitudes represents a major barrier to the introduction of new valuable traits. Any trait (e.g. disease resistance) has to be bred into each of the different maturity groups separately, a laborious and costly exercise. The availability of single strain, which could be grown at any latitude, would therefore greatly increase the potential for introducing new traits to crop species such as soybean and cotton.
For many of the specific effects, traits and utilities listed in Table 4 and Table 6 that may be conferred to plants, one or more transcription factor genes may be used to increase or decrease, advance or delay, or improve or prove deleterious to a given trait. For example, overexpression of a transcription factor gene that naturally occurs in a plant may cause early flowering relative to non-transformed or wild-type plants. By knocking out the gene, or suppressing the gene (with, for example, antisense suppression) the plant may experience delayed flowering. Similarly, overexpressing or suppressing one or more genes can impart significant differences in production of plant products, such as different fatty acid ratios. Thus, suppressing a gene that causes a plant to be more sensitive to cold may improve a plant's tolerance of cold. More than one transcription factor gene may be introduced into a plant, either by transforming the plant with one or more vectors comprising two or more transcription factors, or by selective breeding of plants to yield hybrid crosses that comprise more than one introduced transcription factor.
A listing of specific effects and utilities that the presently disclosed transcription factor genes have on plants, as determined by direct observation and assay analysis, is provided in Table 4. Table 4 shows the polynucleotides identified by SEQ ID NO; Gene ID No. (GD); and if the polynucleotide was tested in a transgenic assay. The first column shows the polynucleotide SEQ ID NO; the second column shows the GID; the third column shows whether the gene was overexpressed (OE) or knocked out (KO) in plant studies; the fourth column shows the category of modified trait resulting from the knock out or overexpression of the polynucleotide in the transgenic plant; and the fifth column (“Experimental Observations”), includes specific observations made with respect to the polynucleotide of the respective first column.





TABLE 4



Traits, trait categories, and effects and utilities that transcription factor genes have on plants.







SEQ ID





NO:
GID
OE/KO
Category
Experimental Observations









1
G2
OE
Flowering time
Late flowering

3
G12
OE/KO
Morphology; altered
Leaf and hypocotyl cell death; knockout seedlings




programmed cell death
germinated in the dark on 1-aminocyclopropane-1-




Growth regulator; altered
carboxylic acid-containing media were more stunted




ethylene sensitivity
than controls




Dev and morph;
Formation of necrotic lesions




Morphology: other

5
G15
OE
Dev and morph; flower
Altered flower morphology



OE
Flowering time
Late flowering

7
G30
OE
Leaf; altered shape
Long cotyledons, petioles and hypocotyls, dark




Leaf; dark green leaves
green, glossy leaves; shade avoidance




Leaf; glossy leaves




Light response; Long




petioles




Light response; Long




hypocotyls




Light response; Long




cotyledons

9
G46
OE
Dev and morph; Size
Increased biomass



OE
Abiotic stress; Drought
Increased tolerance to drought in a soil-based assay

11
G47
OE
Flowering time
Late flowering



OE
Abiotic stress; osmotic stress
Better root growth under osmotic stress



OE
Dev and morph; Architecture
Altered architecture and inflorescence development



OE
Dev and morph; stem
Altered structure of vascular tissues



OE
Abiotic stress; drought
Increased tolerance to drought in a soil-based assay

13
G129
OE
Flowering time
Early flowering




Leaf: altered shape
Leaf shape




Flower: homeotic
Homeotic transformation




transformation

15 and
G131
OE
Dev and morph; size
Small plants

2102


Flowering time
Early flowering



OE
Dev and morph; Leaf
Curled leaves



OE
Dev and morph; Flower
Loss of flower determinacy, terminal flowers



OE
Dev and morph;
Altered inflorescence determinacy




Inflorescence

17
G133
OE
Flower: homeotic
Homeotic transformation




transformation

19
G134
OE
Flower: homeotic
Homeotic transformation




transformation
Increased sensitivity to cold




Abiotic stress; cold




sensitivity

21
G135
OE
Dev and morph; Leaf
Curled leaves



OE
Dev and morph;
Altered inflorescence determinacy; terminal flowers




Inflorescence



OE
Dev and morph; Flower
Loss of flower determinacy,



OE
Flowering time
Early flowering

23
G136
OE
Morphology; altered flower
Altered flower development (tiny petals)




development




Flowering time
Early flowering




Leaf; altered shape
Small, upward curling leaves




Morphology: size
Small plant

25
G137
OE
Flowering time
Early flowering




Inflorescence; Terminal
Terminal flower formation




flowers
Leaf curling




Leaf; altered shape

27
G138
OE
Flowering time
Early flowering

29
G139
OE
Expression; drought
This gene was induced in rosette leaves in response





to drought treatments

31
G140
OE
Flower: homeotic
Homeotic transformation




transformation
Early flowering




Flowering time

33
G142
OE
Flowering time
Early flowering

35
G145
OE
Flowering time
Early flowering




Inflorescence; terminal
Terminal flower




flowers

37
G146
OE
Flowering time
Early flowering

39
G148
OE
Flowering time
Early flowering




Inflorescence; terminal
Terminal flower




flowers

41
G151
OE
Seed; Large seed
Larger seed size than controls

43
G153
OE
Flowering time
Early flowering



OE
Abiotic stress; Nutrient
Altered C/N sensing




uptake

45
G155
OE
Altered sugar sensing
Increased sensitivity to glucose




Flowering time
Early flowering




Abiotic stress; osmotic stress
Increased sensitivity to mannitol




Inflorescence; terminal
Terminal flower




flower

47
G171
OE
Expression; heat
Expression was induced in leaves by heat



OE
Expression; chilling
Expression was induced in leaves by cold



OE
Expression; Fusarium
Expression was induced in leaves by Fusarium

49
G172
OE
Flowering time
Early flowering

51
G173
OE
Flowering time
Late flowering

53
G200
OE
Nutrient; tolerance to low N
Seedlings contained less anthocyanin and were




C/N sensing
greener on high sucrose medium lacking nitrogen,




Leaf; altered shape
and on similar media supplemented with glutamine;




Leaf; light green leaves
mature plants had small, light green pointed leaves;




Flowering time
early flowering

55
G224
OE
Leaf: altered shape
Altered leaf shape




Abiotic stress; cold tolerance
Increased tolerance to cold




Altered sugar sensing
Seedling vigor on high glucose

57
G244
OE
Expression; auxin
Expression was induced by auxin



OE
Expression; drought
Expression was induced by drought



OE
Expression; ABA
Expression was induced by abscisic acid

59
G246
OE
Light response; shade
Shade avoidance




avoidance
Early flowering




Flowering time

61
G253
OE
Size; small plants
Reduced plant size



OE
Dev and morph; Leaf
Heart shaped and dark green leaves



OE
Dev and morph;
Short inflorescence internodes




Inflorescence

63
G268
OE
Plant size; large plants
Increased biomass

65
G287
OE
Dev and morph; Size
Increased biomass

67
G309
OE
Flowering time
Late flowering



OE
Plant size; small plants
Reduced plant size



OE
Leaf altered coloration
Dark green leaves

69
G314
OE
Dev and morph; Size
Increased biomass

71
G319
OE
Plant size; Increased size
Increased size; late flowering; wrinkled, short broad




Flowering time
leaves




Leaf; altered shape

73 and
G324
OE
Flowering time
Late flowering

2103

OE
Size; large plants
Increased biomass

75
G344
OE
Abiotic stress; chilling
More sensitive to chilling in germination assay



OE
Growth regulator; altered
Altered sugar sensing phenotype: more sensitive to




sugar sensing
glucose in a germination assay

77
G351
OE
Altered light response
Leaf orientation and light green coloration

1379
G353
OE
Abiotic stress; osmotic stress
Seedlings were larger and greener on PEG-





containing media

1381
G354
OE
Abiotic stress; drought
Increased tolerance to drought in a soil-based assay

79
G355
OE
Nutrient; Tolerance to low
Enhanced growth under limiting phosphate in root




PO 4
growth assay, and better growth in high NaCl




Abiotic stress; sodium




chloride tolerance

81
G366
OE
Dev and morph; Lethal when
Lethal when overexpressed




overexpressed

83
G370
KO
Abiotic stress; osmotic stress
Short, round leaves; flowers showed a striking




Leaf; altered shape
increase in trichome density on sepals, and carried



OE
Morphology; increased
ectopic trichomes on petals, anthers, and carpels;




trichome density
aerial rosettes occur when a secondary inflorescence




Morphology; altered timing
meristem develops; knockout was more sensitive to




of phase change
osmotic stress in a germination assay and produced





bushy rosettes, small, shiny plants

85
G372
OE
Leaf; altered shape
Altered leaf shape




Flowering time
Late flowering

87
G374
KO
Dev and morph; Embryo
Lethality at early stages of embryo development




lethal

89
G380
OE
Flowering time
Late flowering

91 and
G386
OE
Biochem: misc;
Increased pigment production

2104


Biochemistry: other



OE
Dev and morph; Size
Reduced plant size

93
G416
OE
Flowering time
Early flowering

95
G434
OE
Flowering time
Late flowering

97
G438
OE
Leaf; altered shape
Larger, flatter leaves than those of controls at late




Leaf
stages of development




Plant size; increased size

99
G446
OE
Altered architecture
Altered branching




Leaf; altered shape
Altered leaf shape

101
G468
OE
Leaf; altered shape
Wrinkled leaves

103
G478
OE
Altered light response
Long petioles




Altered sugar sensing
More sensitive to glucose

105
G485
KO
Flowering time
Late flowering



OE
Flowering time
Early flowering

107
G521
OE
Leaf; cell expansion
Cell expansion

109
G549
OE
Dev and morph;
Altered inflorescence determinacy




Inflorescence



OE
Dev and morph; Size
Reduced plant size



OE
Flowering time
Early flowering

111
G550
OE
Morphology; altered flowers
Early flowers were small with poor organ formation,




Abiotic stress; heat tolerance
late flowers were normal; less tolerant to heat stress




Expression; sodium chloride
in a growth assay; high anthocyanin level; G550




Expression; auxin
expression is induced in response to heat, auxin and




Pigment; high anthocyanin
salt stress

113
G571
OE
Hormone sensitivity; altered
This gene is also strongly induced in rosette leaves




ABA response
by ABA, drought, osmotic stress and Erysiphe




Abiotic stress; drought




Abiotic stress; osmotic stress




Expression; Erysiphe

115
G581
OE
Nutrient; tolerance to low N
Overexpressing lines germinated better on plates




Seed; altered coloration
containing low N or plates with low N supplemented




Seed; Large seed
with glutamine, seedlings also had less anthocyanin




Flowering time
accumulation when compared to wild-type controls;




Pigment; Increased
increased seed size, altered seed color; late flowering




anthocyanin

117
G600
OE
Leaf; light green leaves
Small, flat, short and grayish or light green rosette




Flowering time
leaves; early flowering; smaller plants




Plant size; small plants

119 and
G624
OE
Nutrient uptake; tolerance to
Better root growth on media lacking phosphate

2105


low PO 4



OE
Abiotic stress; sodium
Increased tolerance to sodium chloride




chloride tolerance



OE
Increased size
Increased biomass



OE
Flowering time
Late flowering

121
G627
OE
Flowering time
Early flowering

123
G646
OE
Leaf; altered shape
Very narrow downward curled dark green leaves

125 and
G651
OE
Dev and morph; Leaf
Altered leaf shape and gray leaves

2106

OE
Abiotic stress; Cold
Increased sensitivity to cold in a germination assay



OE
Dev and morph; Root
Altered root branching



OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph Flower
Altered flower morphology

127
G652
OE/KO
Leaf biochemistry; ;
Knockout had increase in the leaf glucosinolate




increased glucosinolates
M39480 and seed a-tocopherol, decrease in seed oil;




Seed biochemistry; increased
overexpressor showed delayed senescence




seed prenyl lipids




Seed biochemistry;




decreased seed oil




Delayed senescence

129
G707
OE
Abiotic stress; Nutrient
Altered C/N sensing




uptake



OE
Dev and morph; Leaf
Dark green leaves



OE
Biochem: misc;
Increased pigment production




Biochemistry: other



OE
Flowering time
Late flowering

131
G728
OE
Abiotic stress; cold tolerance
Increased tolerance to cold

133
G730
OE
Dev and morph; Root
Reduced secondary root growth



OE
Dev and morph;
Abaxialization of adaxial surfaces




Morphology: other

135
G738
OE
Flowering time
Late flowering; small seedlings; high anthocyanin




Plant size; small plants
levels in leaf petioles; smaller plants




Pigment; High anthocyanin

137 and
G744
OE
Flowering time
Late flowering

2107

131
G752
OE
Flowering time
Late flowering

141
G807
OE
Abiotic stress; chilling
Seedling vigor was improved in T1 transformants



OE
tolerance
and T2 progeny, seedlings were reproducibly larger,



OE
Expression; heat
grew faster and showed longer hypocotyl and



OE
Expression: auxin
petioles than controls; expression of this gene



OE
Fast growth
moderately upon heat shock and auxin treatment



OE
Light response; long petioles




Light response; long




hypocotyls

143
G811
OE
Leaf; dark green leaves
Dark green leaves




Size; small plants
Reduced size

145
G839
OE
Nutrient; tolerance to low N
Increased tolerance to nitrogen-limited medium




Flowering time
Late flowering

147
G846
OE
Dev and morph Flower
Gamete lethal

149
G852
OE
Flowering time
Late flowering




Size; large plants
Large plant

151
G905
OE
Flowering time
Late flowering




Altered sugar sensing
Seedling vigor on high glucose




Leaf; altered shape
Altered leaf shape

153
G916
OE
Growth regulator; altered
Larger seedlings than wild type in high sucrose,




sugar sensing
seedlings were larger and had less anthocyanin on




Nutrient; tolerance to low N
high sucrose plates that were nitrogen deficient, with




Light response; Long
or without glutamine supplementation;




hypocotyls
disproportionately long hypocotyls and narrow




Morphology; Narrow
cotyledons




cotyledons




Abiotic stress: drought
Increased tolerance to drought in a soil-based assay




tolerance

155
G926
KO
Hormone sensitivity
Reduced sensitivity to ABA



KO
Abiotic stress; Osmotic
Increased tolerance to osmotic stress (salt and




stress
sucrose)



KO
Abiotic stress; Drought
Increased tolerance to drought in a soil-based assay

157
G957
OE
Leaf; altered shape
Wrinkled, curled leaves

159
G961
OE
Dev and morph; Seed
Altered seed development and germination



KO
Seed biochemistry; Seed oil
Increased seed oil

161
G975
OE
Leaf biochemistry; Leaf fatty
Increased wax in leaves




acids




Abiotic stress; Drought
Increased tolerance to drought in a soil-based assay

163
G1011
OE
Morphology; altered flowers
Floral organ abscission was delayed, with stamens,




Leaf; altered shape
petals, and sepals persisting following pollination;




Flowering time
increased trichome density on sepals and ectopic




Morphology; increased
trichomes on carpels; rounded leaves; early




trichome density
flowering

165
G1013
OE
Slow growth
Slow growth rate




Flower alterations
Multiple flower alterations




Altered light response
Light response: leaf orientation




Leaf; altered shape
Altered leaf shape




Altered C/N sensing
C/N sensing: better germination

167
G1017
OE
Abiotic stress; Sodium
Increased tolerance to sodium chloride




chloride tolerance

169
G1033
OE
Premature senescence
Premature leaf senescence




Altered sugar sensing
Increased seedling vigor on sucrose




Abiotic stress; osmotic stress
Increased tolerance to sucrose




tolerance

171 and
G1037
OE
Abiotic stress; Sodium
Increased tolerance to sodium chloride

2108


chloride tolerance

173
G1082
OE
Light response; long
Longer hypocotyls than controls




hypocotyls

175
G1100
OE
Leaf; altered shape
Dark green, pointed leaves, large dark green rosettes;




Leaf; dark green leaves
stunted inflorescence growth and abnormal flowers;




Morphology; altered flowers
slower growth rate; this gene is strongly and




Abiotic stress
specifically induced by drought and salicylic acid




Expression; SA




Expression; drought

177
G1108
OE
Altered sugar sensing
Less sensitive to glucose

179
G1113
OE
Increased plant size
Increased biomass



OE
Flowering time
Late flowering

181
G1128
OE
Leaf; altered shape
Dark green, narrow contorted leaves; premature leaf




Leaf; dark green leaves
and flower senescence; little or no seed development




Morphology; Changes to




flower




Growth rate; altered rate of




senescence




Seed; altered development

183
G1136
OE
Flowering time
Late flowering




Nutrient; sensitivity to low N
Increased sensitivity to nitrogen

185
G1142
OE
Flowering time
Late flowering




Leaf; altered shape
Altered leaf shape

187 and
G1150
OE
Abiotic stress; Nutrient
Altered C/N sensing

2109


uptake



OE
Flowering time
Late flowering



OE
Dev and morph; Size
Increased biomass

189
G1206
OE
Abiotic stress; drought
Increased seedling vigor under drought conditions,




tolerance
seedlings larger and greener than controls

191
G1247
OE
Size; small plant
Altered leaf shape




Leaf; altered shape
Reduced plant size

193
G1274
OE
Abiotic stress; Cold
More tolerant to cold in a germination assay



OE
Abiotic stress; Chilling
More tolerant to chilling in a seedling growth assay



OE
Abiotic stress; Drought
Increased tolerance to drought in a soil-based assay



OE
Dev and morph;
Altered inflorescence architecture




Inflorescence



OE
Abiotic stress; Nutrient
Increased tolerance to low nitrogen




uptake



OE
Abiotic stress; Nutrient
Altered C/N sensing




uptake



OE
Dev and morph; Leaf
Large leaves

195
G1276
OE
Flowering time
Late flowering

197
G1289
OE
Plant size; small
All overexpressing lines showed reduced size

199
G1313
OE
Size; large plant
Increased seedling size

201
G1327
OE
Leaf; dark green leaves
Dark green leaves

203
G1340
OE
Plant size; small
All overexpressing lines showed reduced size

205
G1341
OE
Dev and morph; Leaf
Dark green leaves, leaf curling

207
G1357
OE
Flowering time
Late flowering



OE
Hormone sensitivity
Insensitive to ABA



OE
Abiotic stress; Chilling
More tolerant to chilling stress in a growth assay



OE
Dev and morph; Leaf
Altered leaf shape and dark green leaves



OE
Abiotic stress; Drought
Increased tolerance to drought in a soil-based assay

209
G1361
OE
Flowering time
Late flowering




Leaf; altered shape
Altered leaf shape

211
G1384
OE
Dev and morph; lethal when
Lethal when overexpressed




overexpressed

213
G1389
OE
Leaf; altered shape
Inner rosette leaves were dark green, narrow, and




Leaf; dark green leaves
curled in T1 plants

215
G1412
OE
Abiotic stress; Osmotic
Increased tolerance to osmotic stress




stress



OE
Hormone sensitivity
ABA insensitive

217
G1420
OE
Morphology; long
Long flower organs (sepal and petal), mildly




cotyledons
serrated, narrow, darker green leaves (including




Leaf; altered shape
pedicel); poor growth on glucose; long narrow




Leaf; dark green leaves
cotyledons




Growth regulator; altered




sugar sensing




Morphology; altered flower

219
G1423
OE
Leaf; dark green leaves
Dark green leaf coloration compared to wild-type,




Plant size; small plants
indicating a change in the levels of chlorophyll,





carotenoids, or flavonoids; transformants were





distinctly small

221
G1446
OE
Flowering time
Late flowering

223
G1451
OE
Leaf; large leaves
Large leaf size




Flowering time
Late flowering




Size; large plant
Increased plant size

225
G1452
OE
Flowering time
Late flowering



OE
Dev and morph; Leaf
Altered leaf shape, dark green leaves



OE
Abiotic stress; Osmotic
Better germination on sucrose and salt




stress




Abiotic stress; drought
Increased tolerance to drought in a soil-based assay



OE
Hormone sensitivity
Reduced sensitivity to ABA



OE
Dev and morph; Trichome
Reduced trichome density

227
G1468
OE
Flowering time
Late flowering



OE
Dev and morph; size
Increased biomass



OE
Dev and morph; leaf
Grayish and narrow leaves



OE
Dev and morph;
Slow growth rate




Morphology: other

229
G1474
OE
Flowering time
Late flowering




Inflorescence; altered
Altered inflorescence architecture




architecture
Reduced plant size




Size; small plants

231
G1476
OE
Fast growth
Faster seedling growth rate; rounded, contorted




Leaf; altered shape
leaves

233
G1482
OE
Increased pigment
Increased anthocyanins in leaf

235
G1483
OE
Abiotic stress; Nutrient
Altered C/N sensing




uptake

237
G1493
OE
Altered sugar sensing
Seedling vigor on high glucose




Leaf; altered shape
Altered leaf shape




Flowering time
Late flowering

239
G1507
OE
Expression: altered ABA
This gene is induced by ABA, heat, Fusarium , and




response
salicylic acid




Expression; heat




Expression; Fusarium




Expression: altered response




to SA

241
G1510
OE
Leaf; dark green leaves
Dark green leaves




Altered light response
Long hypocotyls

243
G1535
OE
Slow growth
Slow growth rate




Leaf; altered shape
Altered leaf shape and coloration




Altered sugar sensing
Dark green seedling on high glucose




Altered C/N sensing
C/N sensing: more anthocyanin on nitrogen-limited





media

245
G1538
OE
Flowering time
Early flowering; improved tolerance to salt stress in




Abiotic stress; sodium
a root growth assay; larger seedlings with more




chloride tolerance
secondary root growth than wild-type; longer leaf




Leaf; altered shape
petioles; expression induced in leaves by heat and




Expression; heat
salicylic acid treatments




Expression; SA

247
G1539
OE
Altered trichome structure
Altered trichome structure




Altered cell differentiation
Altered cell differentiation




Flower; altered carpel
Ectopic carpel development




development

249
G1549
OE
Size; small plant
Reduced plant size




Slow growth
Slow growth rate




Leaf; altered shape
Altered leaf shape and coloration

251
G1554
OE
Flowering time
Late flowering



OE
Dev and morph; Leaf
Dark green leaves

253
G1556
OE
Dev and morph; Lethal when
Lethal when overexpressed




overexpressed

255
G1557
OE
Abiotic stress; sodium
Increased tolerance to sodium chloride




chloride tolerance

257
G1585
OE
Dev and morph
Altered cell differentiation




Leaf; altered shape
Altered leaf shape

259
G1591
OE
Flower; altered carpel
Ectopic carpel development




development
Altered cell differentiation




Morphology; altered cell




differentiation

261
G1593
OE
Inflorescence; altered
Altered inflorescence architecture




architecture
Altered leaf shape and coloration




Leaf; altered shape and




coloration

263
G1660
OE
Abiotic stress; sodium
More root growth and seedling vigor in high salt




chloride tolerance

265
G1718
OE
Leaf; altered coloration
Pale gray leaves

267
G1730
OE
Abiotic stress; osmotic stress
Large and green seedlings on mannitol and glucose




tolerance

269
G1743
OE
Inflorescence; altered
Altered inflorescence architecture




architecture
Altered leaf shape, dark green leaves




Leaf; altered shape and




coloration

271
G1753
OE
Altered sugar sensing
Altered inflorescence architecture




Abiotic stress; osmotic stress
Better germination on high sucrose media




tolerance




Inflorescence; altered




architecture

273
G1772
OE
Size; small plant
Reduced plant size

275
G1779
OE
Abiotic stress; chilling
Mature plants have enhanced tolerance to chilling





stress for a long time period

277
G1792
OE
Disease; Erysiphe
Increased resistance to Erysiphe



OE
Disease; Fusarium
Increased resistance to Fusarium



OE
Disease; Botrytis
Increased resistance to Botrytis



OE
Dev and morph; Leaf
Dark green, shiny leaves



OE
Nutrient uptake; tolerance to
Increased tolerance to low nitrogen




low N



OE
Abiotic stress; Drought
Increased tolerance to drought in a soil-based assay

279
G1796
OE
Inflorescence; Short
Flower carpel alterations (thickened club-like




internodes
carpels); short internodes; dark curled leaves




Leaf; altered shape




Leaf; dark green leaves

281
G1797
OE
Flowering time
Early flowering



OE
Dev and morph Flower
Flower organs persisted following fertilization

283
G1798
OE
Flowering time
Early flowering



OE
Dev and morph;
Multiple inflorescence defects




Inflorescence

285
G1808
OE
Abiotic stress; chilling
Mature overexpressing plants were less tolerant to





cold

287
G1816
OE
Trichome; glabrous leaves
Glabrous leaves




Abiotic stress; osmotic stress
Increased tolerance to high glucose




tolerance
Increased root hairs




Root; increased hairs
Increased tolerance to high glucose




Altered sugar sensing
C/N sensing: improved tolerance to low nitrogen




Altered C/N sensing

289
G1823
OE
Flowering time
Early flowering

291
G1825
OE
Flowering time
Early flowering




Leaf; altered shape
Altered leaf shape

293
G1832
OE
Dev and morph; Lethal when
Lethal when overexpressed




overexpressed

295
G1837
OE
Abiotic stress; sodium
More root growth and seedling vigor in high salt




chloride tolerance



OE
Abiotic stress; chilling
Enhanced tolerance, better growth of seedlings




tolerance
under chilling conditions

297
G1840
OE
Dev and morph;
Formation of necrotic lesions




Morphology: other

299
G1846
OE
Leaf; altered shape
Dark green leaves, poorly developed inflorescences




Leaf; dark green leaves

301
G1850
OE
Dev and morph; Lethal when
Lethal when overexpressed




overexpressed

303
G1863
KO
Abiotic stress; sodium
Decreased germination under salt stress




chloride



OE
Leaf; altered shape and
Altered leaf shape and coloration




coloration
Late flowering




Flowering time

305
G1893
OE
Hormone sensitivity; altered
Insensitivity to ABA; rectangular cotyledons;




ABA response; altered
seedlings contain more anthocyanin; leaves were




cotyledons
small with serrated margins




Morphology




Leaf; altered shape

307
G1917
OE
Leaf; altered shape
Leaves are elongated and curled; with frilly, serrated





margins

309
G1923
OE
Abiotic stress; heat
This gene is up-regulated by Fusarium and Erysiphe



OE
Abiotic stress; osmotic stress
infection, as well as auxin, heat and osmotic stress



OE
Expression; Fusarium
treatments



OE
Expression; Erysiphe



OE
Hormone sensitivity; auxin

311
G1928
OE
Abiotic stress; cold tolerance
Increased tolerance to cold

313
G1932
OE
Leaf; altered shape
Leaves were dark green with jagged leaf margins




Leaf; dark green leaves

315
G1938
OE
Leaf; altered shape
Curled, contorted leaves, dark green leaves; slow




Leaf; dark green leaves
growth rate; more sensitive to osmotic stress




Abiotic stress; osmotic stress




Plant size; small plants

317
G1945
OE
Leaf; altered shape
Altered leaf shape




Flowering time
Late flowering

319
G1957
OE
Dev and morph; Lethal when
Lethal due to meristem defects




overexpressed

321
G1968
OE
Nutrient; tolerance to low N
Overexpression resulted in more tolerance to chilling





stress in a growth assay compared to control plants;





overexpressing lines contained more anthocyamns





when grown under low nitrogen, or low nitrogen





plus glutamate, in a germination assay

323
G1983
OE
Leaf; altered shape
Dark green leaves; late flowering; small plants




Leaf; dark green leaves




Flowering time




Size; small plants

325
G1985
OE
Dev and morph; phase
Phase change and floral reversion




change and floral reversion
Aerial rosettes




Dev and morph; aerial




rosettes

327
G1988
OE
Nutrient; Tolerance to low N
Better growth on low nitrogen plus glutamine, better




Nutrient; Tolerance to low
growth on low phosphate; long hypocotyl, long




PO 4
petiole, early flowering




Flowering time




Light response; Long petiole




Light response; Long




hypocotyl

329
G1990
OE
Dev and morph;
Lethal when overexpressed




Morphology: other

331
G1993
OE
Leaf; altered shape
Short petioles and round leaf shape




Size; small plant size
Reduced plant size

333
G1995
OE
Morphology; increased
Increased trichome number on sepals, ectopic




trichome number
trichomes on carpels yield enhanced production of




Nutrient; Tolerance to low N
leaf, flower, and outer ovule epidermis products;




Nutrient; Tolerance to low
slightly less tolerant to low nitrogen and low




PO 4
phosphorus; aerial rosettes occurred when a




Inflorescence; altered aerial
secondary mflorescence meristem developed in a




rosettes
manner comparable to a primary shoot meristem




Morphology; altered timing
during the vegetative phase of growth, with aerial




of phase change
rosette-like structures and floral organs being bract-





like

335
G1998
OE
Flowering time
Late flowering

337
G1999
OE
Flowering time
Late flowering

339
G2035
OE
Abiotic stress; sodium
Increased seedling vigor in high sodium chloride




chloride tolerance

341 and
G2041
OE
Abiotic stress; Sodium
Increased tolerance to sodium chloride

2110


chloride tolerance

343
G2051
OE
Abiotic stress; Cold
Increased tolerance to cold in a germination assay

345
G2060
OE
Abiotic stress; sodium
More root growth and seedling vigor in high salt




chloride tolerance

347
G2063
OE
Abiotic stress; cold tolerance
Increased seedling vigor in cold

349
G2070
OE
Abiotic stress; chilling
Mature overexpressing plants were less tolerant to



OE
Abiotic stress; heat
cold; gene was induced by ABA, cold and heat



OE
Expression; altered ABA




response

351
G2071
OE
Flowering time
Early flowering

353
G2084
OE
Leaf; altered shape
Short petioles, and rounded, slightly dark green





leaves

355
G2085
OE
Leaf; altered shape and
Altered leaf shape, dark green leaves




coloration




Seed; increased size, altered
Increased seed size, altered seed color




color




Trichome; increased density
Increased trichome density

357 and
G2106
OE
Flowering time
Late flowering

2111

359
G2109
OE
Hormone sensitivity; altered
Much less sensitive to ABA in a germination assay




ABA response
than wild-type

361
G2111
OE
Sugar sensing; Sugar sensing
Altered sugar sensing response; decreased growth





and small, pale seedlings on glucose medium

363
G2129
OE
Flowering time
Early flowering

1495
G2133
OE
Abiotic stress; drought
Increased tolerance to drought in a soil-based assay

365
G2142
OE
Abiotic stress; tolerance to
More tolerant to phosphate deprivation in a root




low PO 4
growth assay



OE
Flowering time
Accelerated flowering time

367
G2146
OE
Hormone sensitivity; altered
Insensitive to ABA in a germination assay; late




ABA response
flowering; more branching, short internodes,




Flowering time
inflorescences were shorter and bushier than wild




Inflorescence; Short
type; dark green appearance




internodes




Leaf; dark green leaves

369
G2184
OE
Flowering time
Early flowering

371
G2207
OE
Hormone sensitivity; altered
Increased tolerance to osmotic stress under high salt




ABA response Abiotic
or sucrose and less sensitive to ABA in germination




stress; sodium chloride
assays; late flowering; narrow dark green leaves




tolerance




Abiotic stress; osmotic




tolerance




Flowering time




Leaf; altered shape




Leaf; dark green leaves

373
G2213
OE
Dev and morph;
Lethal when overexpressed




Morphology: other

375
G2226
OE
Inflorescence; altered
Altered inflorescence architecture




architecture
Reduced plant size




Size; small plants
Altered leaf shape, dark green leaves




Leaf; altered shape and




coloration

377
G2227
OE
Size; small plant size
Reduced plant size




Leaf; altered shape
Altered leaf shape

379
G2239
OE
Altered C/N sensing
C/N sensing: Better germination on low nitrogen





with sucrose or sucrose plus glutamine

381
G2251
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Round and dark green leaves



OE
Dev and morph;
Short inflorescence internodes




Inflorescence



OE
Flowering time
Late flowering

383
G2269
OE
Flowering time
Late flowering

385
G2298
OE
Dev and morph; Lethal when
Lethal when overexpressed




overexpressed

387
G2311
OE
Flowering time
Early flowering

389
G2317
OE
Abiotic stress; cold tolerance
Increased tolerance to cold




Abiotic stress; sodium
Increased seedling vigor on high sodium chloride




chloride

391 and
G2319
OE
Abiotic stress; Sodium
Increased tolerance to sodium chloride

2112


chloride tolerance



OE
Flowering time
Late flowering

393
G2334
OE
Dev and morph; Size
Increased biomass



OE
Flowering time
Late flowering



OE
Dev and morph; Leaf
Dark green leaves and altered leaf shape

395
G2371
OE
Leaf; altered coloration
Dark green leaves




Seed; altered coloration
Altered seed coloration

397
G2372
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Dark green leaves



OE
Flowering time
Early flowering



OE
Dev and morph;
Altered inflorescence determinacy and reduced




Inflorescence
fertility

399
G2375
OE
Leaf; altered shape
Small, narrow leaves; plants were distinctly smaller




Plant size; small plants
than wild type

401
G2382
OE
Hormone sensitivity; altered
Insensitive to ABA in germination assays




ABA response

403
G2394
OE
Abiotic stress; sodium
Enhanced germination on high sodium chloride




chloride tolerance

405
G2404
OE
Abiotic stress; sodium
Enhanced root growth on high sodium chloride




chloride tolerance

407
G2432
OE
Light response; altered shade
Shade avoidance; narrow, upward pointing leaves;




avoidance
delayed flowering, infertile flowers; narrow




Leaf; altered shape
cotyledons; poorly developed roots




Flowering time




Morphology; Narrow




cotyledons

409
G2443
OE
Flowering time
Early flowering

411,
G2453
OE
Biochem: misc;
Increased pigment production

2113


Biochemistry: other

and

OE
Abiotic stress; Sodium
Increased tolerance to sodium chloride

2114


chloride tolerance



OE
Dev and morph; Leaf
Altered leaf shape and dark green leaves



OE
Dev and morph; Size
Reduced plant size

413
G2455
OE
Leaf; altered shape
Leaves are narrow and curled downward

415
G2456
OE
Dev and morph; Leaf
Curled and dark green leaves



OE
Biochem: misc;
Increased pigment production




Biochemistry: other



OE
Dev and morph; Size
Reduced plant size

417
G2457
OE
Flower alterations
Multiple flower alterations




Leaf; altered shape
Altered leaf shape




Abiotic stress; sodium
Increased root growth and less bleaching on high




chloride tolerance
sodium chloride

419
G2459
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Curled leaves



OE
Biochem: misc;
Increased pigment production




Biochemistry: other

421
G2467
OE
Premature senescence
Early senescence

423
G2492
OE
Size; small plants
Reduced plant size

425
G2505
OE
Abiotic stress; drought
Increased tolerance to drought in a soil-based assay




tolerance

427
G2515
OE
Flowering time
Early flowering



OE
Dev and morph;
Altered inflorescence determinacy




Inflorescence



OE
Dev and morph Flower
Altered flower morphology



OE
Dev and morph; Size
Reduced size

429
G2525
OE
Abiotic stress; cold
Increased sensitivity to cold




sensitivity

431
G2536
OE
Leaf; large leaf size
Large leaf size




Size; large plant size
Increased plant size




Delayed senescence
Delayed senescence

433
G2543
OE
Abiotic stress; cold
Increased sensitivity to cold




sensitivity

435
G2550
OE
Leaf; altered shape and
Altered leaf shape, dark green leaves




coloration
Altered inflorescence architecture




Inflorescence; altered




architecture

437 and
G2559
OE
Flowering time
Late flowering

2115

439
G2565
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Grayish leaf coloration and altered leaf shape

441
G2567
OE
Abiotic stress; chilling
Enhanced tolerance, better growth under chilling




tolerance
conditions

443
G2570
OE
Dev and morph;
Lethal when overexpressed




Morphology: other

445
G2571
OE
Inflorescence; Branching
Changes in coloration, branching patterns, and leaf




changes
and flower development, branching, sympodial in




Leaf; altered shape
the inflorescence, similar to that shown by tomato





plants

447
G2574
OE
Premature senescence
Premature leaf senescence




Size; small plants
Reduced plant size

449
G2575
OE
Dev and morph; Leaf
Altered leaf shape



OE
Dev and morph;
Altered inflorescence architecture




Inflorescence

451
G2579
OE
Dev and morph; Silique
Altered silique size and shape



OE
Dev and morph Flower
Increased carpel size and infertile



OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Altered leaf shape



OE
Abiotic stress; Chilling
Increased tolerance to chilling in a plate-based





growth assay

453
G2585
OE
Seed; Large seed
Larger seed size than controls

455
G2587
OE
Dev and morph; lethal when
Lethal when overexpressed




overexpressed

457
G2592
OE
Abiotic stress; cold
Increased sensitivity to cold




sensitivity

459
G2597
OE
Abiotic stress; chilling
This gene was induced in leaf tissue following cold





treatments at 4° C.

461
G2603
OE
Abiotic stress; cold
Increased sensitivity to cold




sensitivity

463
G2604
OE
Abiotic stress; Nutrient
Altered C/N sensing




uptake



OE
Flowering time
Late flowering



OE
Dev and morph; Leaf
Altered leaf surface, gray leaves

465
G2616
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph;
Altered inflorescence architecture and flower




Inflorescence
development

467
G2617
OE
Hormone sensitivity; altered
More ABA insensitive than wild-type in a




ABA response
germination assay; faster growth rate for seedlings




Fast growth
and early stage plants; short petioles, short pedicels




Leaf; altered shape
and wrinkled, curled, rounded leaves

469
G2628
OE
Flowering time
Early flowering



OE
Leaf; altered shape
Rounded leaves;



OE
Plant size; small plants
Small plants

471
G2632
OE
Abiotic stress; Chilling
Increased sensitivity to chilling in a growth-based





assay

473
G2633
OE
Flowering time
Early flowering

475
G2636
OE
Leaf; altered shape
Alterations in the pattern of rosette leaf initiation by




Morphology; altered
the shoot meristem; lobed leaves; adventitious




meristem development
shoots on the adaxial surface of lobed cotyledons




Morphology; lobed




cotyledons

477 and
G2639
OE
Dev and morph;
Short inflorescence internodes

2116


Inflorescence



OE
Flowering time
Early flowering



OE
Dev and morph Flower
Altered flower morphology and poorly fertile

479
G2640
OE
Dev and morph Flower
Altered flower morphology and poor fertility



OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Dark green leaves with glossy surfaces



OE
Dev and morph;
Short inflorescence internodes




Inflorescence

481
G2649
OE
Dev and morph;
Short inflorescence internodes




Inflorescence



OE
Dev and morph; Leaf
Dark green, glossy leaf surface and elongated leaf





shape



OE
Dev and morph Flower
Altered flower morphology and poorly fertile



OE
Dev and morph; Size
Reduced plant size

483
G2650
OE
Flowering time
Early flowering; T2 plants developed excessive




Light response; Long
numbers of small axillary rosette leaves, long narrow




petioles
leaves; elongated petioles; long hypocotyls; leaves




Light response; Long
were held in a more upright orientation than controls




hypocotyls
(potential shade avoidance); larger seedlings and




Light response; Upright
mature plants than controls; larger seedlings than




leaves
controls under chilling conditions; increased number




Abiotic stress; chilling
of axillary meristems in the rosettes




tolerance




Size; Increased plant size




Morphology; More




meristems

485
G2655
OE
Dev and morph; Root
Poorly developed and greenish roots

487
G2661
OE
Growth regulator; altered
Better germination on glucose with greener




sugar sensing
cotyledons than wild-type; darker plants




Leaf; dark green leaves

489 and
G2679
OE
Dev and morph;
Enhanced seedling vigor

2117


Morphology: other

491
G2682
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Curled leaves

493
G2686
OE
Leaf; altered shape
Rounded leaves with slightly lobed margins

495
G2690
OE
Leaf; altered shape
Narrow, dark green leaves that roll down at the




Leaf; dark green leaves
margins

497
G2691
OE
Abiotic stress; sodium
Higher germination in high salt




chloride tolerance

499
G2694
OE
Flowering time
Late flowering




Size; increased size
Increased seedling size




Leaf; altered shape and
Altered inflorescence architecture




coloration
Altered leaf shape, dark green leaves




Dev and morph; flower
Multiple flower alterations




alterations
Long petioles and leaf orientation




Inflorescence; altered




inflorescence architecture




Altered light response

501
G2699
OE
Leaf; altered shape and size
Long petioles and large leaves

503
G2702
OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph; Leaf
Altered leaf shape

505
G2717
OE
Abiotic stress; Osmotic
Increased tolerance to osmotic stress (salt and




stress
sucrose)



OE
Abiotic stress; sodium




chloride tolerance



OE
Abiotic stress; drought
Increased tolerance to drought in a soil-based assay




tolerance



OE
Hormone sensitivity; altered
Insensitive to ABA in germination assays




ABA response



OE
Size; increased plant size
Larger seedlings

507
G2718
OE
Dev and morph; Root
Increased root hair density



OE
Dev and morph; Trichome
Reduced trichome density



OE
Abiotic stress; Nutrient
Increased tolerance to low nitrogen




uptake



OE
Biochem: misc;
Reduced pigment production




Biochemistry: other

509
G2723
OE
Flowering time
Late flowering

511
G2741
OE
Flowering time
Late flowering



OE
Dev and morph; Size
Increased biomass

513
G2743
OE
Morphology; altered flower
Delayed flowering; altered flower development




development
(sepals, petals and stamens were reduced in size,




Flowering time
pollen production was poor)

515
G2747
OE
Root; reduced root formation
Long petioles and slightly narrow elongated leaf




Leaf; altered shape
blades, little or no secondary root formation

517
G2754
OE
Dev and morph
Shade avoidance




Flowering time
Early flowering

519
G2757
OE
Size; small plant size
Reduced plant size

521
G2763
OE
Flowering time
Late flowering



OE
Abiotic stress; chilling
More sensitive to chilling temperatures during





growth



OE
Growth regulator; altered
More sensitive to glucose




sugar sensing



OE
Pigment; high anthocyanin
More anthocyanin accumulation in seedlings



OE
Leaf; dark green leaves
Dark green leaves

523
G2765
OE
Slow growth
Retarded growth at early stages

525 and
G2768
OE
Dev and morph; Leaf
Increased leaf size

2118

OE
Dev and morph Flower
Increased petal number, loss of floral determinacy

527 and
G2771
OE
Dev and morph; Leaf
Altered leaf shape and dark green leaves

2119

OE
Abiotic stress; Chilling
Reduced anthocyanins in a chilling growth assay



OE
Flowering time
Late flowering



OE
Dev and morph; Light
Elongated hypocotyl and pale in coloration




response

529
G2776
OE
Abiotic stress; osmotic
Seedlings grown on high sucrose were larger with




tolerance
green cotyledons compared with wild-type seedlings




Growth regulator; altered




sugar sensing

531
G2777
OE
Flowering time
Early flowering

533
G2779
OE
Dev and morph
Pale leaf coloration




Flowering time
Early flowering

535
G2783
OE
Premature senescence
Early senescence




Size; small plant
Reduced plant size

537 and
G2784
OE
Dev and morph;
Altered inflorescence architecture

2120


Inflorescence



OE
Dev and morph;
Slow growth rate




Morphology: other



OE
Dev and morph; Leaf
Dark green and curled leaves



OE
Abiotic stress; Cold
Increase tolerance to cold in a germination assay

539
G2790
OE
Abiotic stress; chilling
Mature overexpressing plants were less tolerant to





cold

541 and
G2802
OE
Flowering time
Early and late flowering

2121

543
G2805
OE
Flowering time
Early flowering

545
G2826
OE
Morphology; increased
Flowers had increased trichome density on sepals




trichome density
and possessed ectopic trichomes on the carpels;




Inflorescence; ectopic aerial
overexpressors developed aerial rosettes at




rosettes
coflorescence nodes, indicating a disruption in phase




Morphology; altered timing
change in the inflorescence




of phase change

547
G2830
OE
Altered C/N sensing
C/N sensing

549
G2832
OE
Flowering time
Early flowering




Leaf; altered coloration
Pale gray leaf color

551
G2834
OE
Slow growth rate
Slow growth rate

553
G2837
OE
Leaf; altered shape and
Altered leaf shape, dark green leaves




coloration

555
G2838
OE
Flowering time
Late flowering




Size; large plant
Increased seedling size




Leaf; altered coloration
Aerial rosettes




Trichome; increased density
Dark green leaves




Flower; multiple alterations
Increased trichome density





Multiple flower alterations

557
G2839
OE
Abiotic stress; osmotic stress
Better germination on high sucrose; increased




tolerance
resistance to osmotic stress; small, contorted leaves



OE
Leaf; altered shape
that are upcurled at margins, short petioles; poorly



OE
Growth regulator: altered
developed flowers with downward-pointing short




sugar sensing
pedicels



OE
Inflorescence; Architectural




change

559
G2846
OE
Leaf; altered shape and
Altered leaf shape, dark green leaves




coloration
Late flowering




Flowering time
Reduced plant size




Size; small plant

561
G2847
OE
Leaf; altered coloration
Dark green leaves




Size; small plant
Reduced plant size

563
G2850
OE
Leaf; altered shape and
Curled, dark green leaves




coloration

565
G2851
OE
Leaf; small leaves
Small, dark green, curled and wrinkled leaves; small




Leaf; altered shape
plants, slow growing




Leaf; dark green leaves




Slow growing

567
G2854
OE
Hormone sensitivity; altered
Better germination on high ABA and sucrose-




ABA response
containing media




Growth regulator; altered




sugar sensing

569
G2859
OE
Leaf; altered shape and
Altered leaf shape and light green leaves




coloration
Inflorescence architecture




Inflorescence; altered
Long hypocotyls, cotyledons; light green plants




architecture




Altered light response

571
G2865
OE
Hormone sensitivity; altered
Insensitive to ABA in germination assays




ABA response

573
G2866
OE
Dev and morph; Leaf
Curled leaves

575
G2869
OE
Dev and morph;
Lethal when overexpressed




Morphology: other

577
G2884
OE
Dev and morph;
Abnormal embryo development




Morphology: other



OE
Dev and morph; Size
Reduced plant size



OE
Dev and morph Flower
Multiple flower defects and low fertility



OE
Dev and morph; Light
Long and green hypocotyls




response

579
G2885
OE
Dev and morph; cell
Altered cell differentiation




differentiation
Decreased tolerance to cold




Abiotic stress; cold tolerance

581
G2887
OE
Dev and morph; Lethal when
Lethal when overexpressed




overexpressed

583
G2888
OE
Leaf; altered shape
Altered leaf shape

585
G2898
OE
Sugar sensing
Better germination on high glucose media

587 and
G2907
OE
Dev and morph; Senescence
Accelerated senescence

2122

589
G2913
OE
Nutrient; tolerance to low N
Less anthocyanin on nitrogen-limited media

591
G2930
OE
Abiotic stress; chilling
Mature plants have enhanced tolerance to chilling




tolerance
stress

593
G2933
OE
Seed; Large seed
Big seeds; larger plants; more tolerant to chilling




Abiotic stress; chilling
stress in growth assays




tolerance

595
G2934
OE
Size; small plant
Reduced plant size

597
G2958
OE
Inflorescence; altered
Altered inflorescence architecture




architecture
Altered leaf shape, dark green leaves




Leaf; altered shape and
Reduced plant size




coloration




Size; small plants

599
G2964
OE
Flowering time
Late flowering




Dev and morph; aerial
Aerial rosettes




rosettes

601
G2967
OE
Flowering time
Early flowering

603
G2969
OE
Hormone sensitivity; altered
Increased tolerance to sucrose and ABA in




ABA response
germination assays

605
G2972
OE
Nutrient; Tolerance to low
Overexpressing lines had more tolerance to low




PO 4
phosphate conditions

607
G2979
OE
Flowering time
Late flowering



OE
Dev and morph; Size
Increased biomass



OE
Dev and morph Flower
Increased flower organ size and number

609
G2981
OE
Nutrient; Tolerance to low N
Greener, larger seedlings on low nitrogen medium





supplemented with glutamine

611
G2982
OE
Abiotic stress; drought
Plants transformed with this gene displayed




tolerance
increased tolerance to dehydration stress in a soil-





based assay

613
G2983
OE
Flower; ectopic carpel
Ectopic carpel formation




formation
Altered cell proliferation




Dev and morph; altered cell
Altered growth pattern, proliferation and root hair




proliferation
density




Altered root
Altered cell differentiation, trichome cell fate




Dev and morph; altered cell




differentiation, trichome cell




fate

615
G2990
OE
Nutrient; tolerance to low N
Altered response to nitrogen deprivation, including





more root growth and more anthocyanin production





in some lines, more bleaching in others when grown





on low nitrogen, indicating this gene is involved in





the response to nutrient limitation

617
G2992
OE
Hormone sensitivity; altered
Enhanced ability to germinate on high NaCl and




ABA response
high ABA; less tolerant to low nitrogen; early




Flowering time
flowering; fewer lateral roots; altered leaf shape;




Abiotic stress; sodium
smaller plants




chloride tolerance




Nutrient; Tolerance to low N




Root; Fewer lateral roots




Leaf; altered shape




Plant size; small plants

619
G2993
OE
Dev and morph; Light
Elongated hypocotyl and altered leaf orientation




response



OE
Dev and morph; Root
Altered root branching



OE
Flowering time
Late flowering



OE
Abiotic stress; Osmotic
Increased sensitivity to osmotic stress




stress



OE
Abiotic stress; Chilling
Increased sensitivity to chilling in a growth assay

621
G2996
OE
Abiotic stress; osmotic stress
Increased sensitivity to mannitol in root growth





inhibition assays, (no secondary root growth)





indicating this gene influences osmotic stress





response

623
G2998
OE
Abiotic stress; sodium
Better germination in high NaCl; late flowering




chloride tolerance

625
G2999
OE
Abiotic stress; sodium
Increased tolerance to high sodium chloride




chloride tolerance



OE
Abiotic stress: drought
Increased tolerance to drought in a soil-based assay




tolerance



OE
Flowering time

627
G3002
OE
Flowering time
Early flowering

629 and
G3003
OE
Flowering time
Late flowering

2123

631
G3008
OE
Leaf size
Large leaf size

633
G3017
OE
Size; small plant size
Reduced plant size

635
G3021
OE
Flowering time
Late flowering




Leaf; altered shape and
Altered inflorescence architecture




coloration
Altered leaf shape, dark green leaves




Inflorescence; altered




architecture

637
G3032
OE
Dev and morph; Light
Altered leaf orientation




response



OE
Flowering time
Early flowering

639
G3044
OE
Flowering time
Early flowering



OE
Leaf; altered shape
Narrow, serrated leaves



OE
Leaf; light green leaves
Pale leaves

641
G3054
OE
Hormone sensitivity; altered
Reduced sensitivity to ABA




ABA response

643
G3055
OE
Hormone sensitivity; altered
Insensitive to ABA in germination assays




ABA response

645
G3059
OE
Dev and morph; Senescence
Accelerated senescence



OE
Dev and morph; Leaf
Dark green leaves and altered leaf shape



OE
Dev and morph;
Altered inflorescence architecture




Inflorescence



OE
Dev and morph;
Altered cotyledon shape




Morphology: other



OE
Dev and morph; Size
Reduced plant size

647
G3060
OE
Flowering time
Some lines flowered early, and others flowered late

649
G3061
OE
Flowering time
Early flowering

651
G3067
OE
Hormone sensitivity; altered
Insensitive to ABA in germination assays




ABA response

653
G3070
OE
Dev and morph; Leaf
Gray leaf coloration

655
G3076
OE
Abiotic stress; Drought
Increased tolerance to drought

657
G3083
OE
Abiotic stress; sodium
Higher germination in high salt




chloride tolerance

659
G3084
OE
Leaf; altered shape
Altered leaf shape

661
G3086
OE
Flowering time
Early flowering




Abiotic stress; heat tolerance
Increased tolerance to heat




Abiotic stress; sodium
Increased tolerance to high sodium chloride




chloride tolerance




Abiotic stress: drought
Increased tolerance to drought in a soil-based assay




tolerance

663
G3091
OE
Dev and morph;
Retarded growth rate




Morphology: other



OE
Dev and morph; Leaf
Altered leaf shape and dark green leaves

665
G3094
OE
Dev and morph; Leaf
Serrated leaves and long petioles



OE
Dev and morph Flower
Altered flower morphology

667
G3095
OE
Dev and morph; Leaf
Altered leaf shape and dark green leaves



OE
Dev and morph;
Slow growth rate




Morphology: other

669
G3111
OE
Dev and morph; Leaf
Altered leaf shape and dark green leaves



OE
Flowering time
Late flowering



OE
Dev and morph; Senescence
Accelerated senescence



















































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































Table 5 shows the polypeptides identified by SEQ ID NO; Gene ID (GID) No; the transcription factor family to which the polypeptide belongs, and conserved domains of the polypeptide. The first column shows the polypeptide SEQ ID NO; the third column shows the transcription factor family to which the polynucleotide belongs; and the fourth column shows the amino acid residue positions of the conserved domain in amino acid (AA) coordinates.





TABLE 5



Gene families and conserved domains








Conserved




Domains

Polypeptide

in Amino Acid

SEQ ID NO:
GID No.
Coordinates
Family








2
G2
129–195, 221–288
AP2

4
G12
27–94
AP2

6
G15
281–357, 383–451
AP2

8
G30
17–35
AP2

10
G46
107–175
AP2

12
G47
11–80
AP2

14
G129
18–73
MADS

16
G131
1–57
MADS

18
G133
1–57
MADS

20
G134
1–57
MADS

22
G135
1–57
MADS

24
G136
18–74
MADS

26
G137
1–57
MADS

28
G138
1–57
MADS

30
G139
1–57
MADS

32
G140
16–72
MADS

34
G142
2–57
MADS

36
G145
1–57
MADS

38
G146
1–57
MADS

40
G148
1–57
MADS

42
G151
2–57
MADS

44
G153
1–57
MADS

46
G155
1–57
MADS

48
G171
1–57
MADS

50
G172
12–68
MADS

52
G173
1–57
MADS

54
G200
12–116
MYB-(R1)R2R3

56
G224
7–114
PMR

58
G244
14–114
MYB-(R1)R2R3

60
G246
57–159
MYB-(R1)R2R3

62
G253
16–116
MYB-(R1)R2R3

64
G268
186–689
AKR

66
G287
293–354
MISC

68
G309
226–506
SCR

70
G314
54–300
SCR

72
G319
12–42
Z-CO-like

74
G324
245–291
RING/C3H2C3

76
G344
166–192
GATA/Zn

78
G351
77–97, 118–140
Z-C2H2

1380
G353
41–61, 84–104
Z-C2H2

1382
G354
42–62, 88–109
Z-C2H2

80
G355
49–69, 94–116
Z-C2H2

82
G366
40–60
Z-C2H2

84
G370
97–117
Z-C2H2

86
G372
141–180
RING/C3HC4

88
G374
35–67, 286–318
Z-ZPF

90
G380
637–677
RING/C3H2C3

92
G386
133–193
HB

94
G416
451–511
HB

96
G434
39–99
HB

98
G438
22–85
HB

100
G446
53–389
ARF

102
G468
86–102, 141–171
IAA

104
G478
186–281
SBP

106
G485
21–116
CAAT

108
G521
7–156
NAC

110
G549
1–395
MISC

112
G550
134–180
Z-Dof

114
G571
160–220, 441–452
bZIP

116
G581
339–396
HLH/MYC

118
G600
115–290
DBP

120
G624
327–406
ABI3/VP-1

122
G627
1–57
MADS

124
G646
55–97
Z-Dof

126
G651
5–31, 162–182, 208–231
Z-C2H2

128
G652
28–49, 137–151, 182–196
Z-CLDSH

130
G707
109–169
HB

132
G728
206–255
GARP

134
G730
169–217
GARP

136
G738
351–393
Z-Dof

138
G744
176–217
RING/C3H2C3

140
G752
439–479
RING/C3H2C3

142
G807
12–76
HS

144
G811
18–108
HS

146
G839
60–185, 290–353
AKR

148
G846
222–531, 679–719,
SWI/SNF



840–923

150
G852
225–593
SCR

152
G905
118–159
RING/C3H2C3

154
G916
293–349
WRKY

156
G926
174–226
CAAT

158
G957
12–182
NAC

160
G961
12–180
NAC

162
G975
4–71
AP2

164
G1011
2–57
MADS

166
G1013
114–170
WRKY

168
G1017
9–382
ARF

170
G1033
52–123
HMG

172
G1037
11–134, 200–248
GARP

174
G1082
1–53, 503–613
BZIPT2

176
G1100
96–137
RING/C3H2C3

178
G1108
363–403
RING/C3H2C3

180
G1113
85–128
RING/C3H2C3

182
G1128
181–247
AT-hook

184
G1136
397–474
HLH/MYC

186
G1142
63–123
HLH/MYC

188
G1150
887–907
PAZ

190
G1206
494–668
ENBP

192
G1247
18–141
MYB-(R1)R2R3

194
G1274
111–164
WRKY

196
G1276
158–224, 250–316
AP2

198
G1289
207–286, 464–493
AKR

200
G1313
32–135
MYB-(R1)R2R3

202
G1327
14–116
MYB-(R1)R2R3

204
G1340
54–142
TH

206
G1341
1–34, 288–398
BZIPT2

208
G1357
16–153
NAC

210
G1361
59–200
NAC

212
G1384
127–194
AP2

214
G1389
30–87
TEO

216
G1412
13–162
NAC

218
G1420
221–280
WRKY

220
G1423
6–62
MADS

222
G1446
1–405
MISC

224
G1451
22–357
ARF

226
G1452
30–177
NAC

228
G1468
95–115, 170–190
Z-C2H2

230
G1474
41–68
Z-C2H2

232
G1476
37–57
Z-C2H2

234
G1482
5–63
Z-CO-like

236
G1483
17–66
Z-CO-like

238
G1493
242–289
GARP

240
G1507
219–247
RING/C3HC4

242
G1510
230–263
GATA/Zn

244
G1535
109–169
HB

246
G1538
66–126
HB

248
G1539
76–136
HB

250
G1549
75–135
HB

252
G1554
238–287
GARP

254
G1556
19–67
GARP

256
G1557
19–67
GARP

258
G1585
55–115
HB

260
G1591
8–68
HB

262
G1593
227–290
HB

264
G1660
362–476
DBP

266
G1718
113–153
RING/C3H2C3

268
G1730
103–144
RING/C3H2C3

270
G1743
94–136
RING/C3H2C3

272
G1753
12–80
AP2

274
G1772
123–176
RING/C3HC4

276
G1779
190–239
GATA/Zn

278
G1792
17–85
AP2

280
G1796
54–121
AP2

282
G1797
1–57
MADS

284
G1798
1–57
MADS

286
G1808
140–200
bZIP

288
G1816
31–81
MYB-related

290
G1823
205–252
GARP

292
G1825
55–103
GARP

294
G1832
67–87, 150–166, 213–233
Z-C2H2

296
G1837
1–53, 398–507
BZIPT2

298
G1840
87–154
AP2

300
G1846
16–83
AP2

302
G1850
56–149
HS

304
G1863
77–186
GRF-like

306
G1893
73–185
Z-C2H2

308
G1917
153–179
GATA/Zn

310
G1923
23–153
NAC

312
G1928
101–121, 178–198
Z-C2H2

314
G1932
9–76
AP2

316
G1938
74–143
PCF

318
G1945
49–71
AT-hook

320
G1957
52–143
ABI3/VP-1

322
G1968
64–84, 368–390
Z-C2H2

324
G1983
71–147
Z-C3H

326
G1985
37–57
Z-C2H2

328
G1988
5–50
Z-CO-like

330
G1990
184–204, 261–283
Z-C2H2

332
G1993
23–43
Z-C2H2

334
G1995
93–113
Z-C2H2

336
G1998
5–71
Z-CO-like

338
G1999
15–55
Z-CO-like

340
G2035
93–259
AKR

342
G2041
670–906, 1090–1175
SWI/SNF

344
G2051
7–158
NAC

346
G2060
204–263
WRKY

348
G2063
7–63
MADS

350
G2070
45–137
bZIP

352
G2071
307–358
bZIP

354
G2084
41–172
RING/C3HC4

356
G2085
214–241
GATA/Zn

358
G2106
56–139, 165–233
AP2

360
G2109
1–57
MADS

362
G2111
1–57
MADS

364
G2129
71–140
bZIP

1496
G2133
11–83
AP2

366
G2142
43–120
HLH/MYC

368
G2146
136–200
HLH/MYC

370
G2184
17–147
NAC

372
G2207
180–227, 546–627
bZIP-NIN

374
G2213
156–205
bZIP-NIN

376
G2226
103–144
RING/C3H2C3

378
G2227
199–239
RING/C3H2C3

380
G2239
128–169
RING/C3H2C3

382
G2251
89–132
RING/C3H2C3

384
G2269
136–177
RING/C3H2C3

386
G2298
4–71
AP2

388
G2311
5–58
MYB-related

390
G2317
48–110
MYB-related

392
G2319
32–120
MYB-related

394
G2334
82–118, 150–194
GRF-like

396
G2371
25–127
ABI3/VP-1

398
G2372
18–378
ARF

400
G2375
51–148
TH

402
G2382
90–177, 246–333
TH

404
G2394
355–395
RING/C3H2C3

406
G2404
319–359
RING/C3H2C3

408
G2432
64–106
Z-Dof

410
G2443
20–86
Z-CO-like

412
G2453
130–176
YABBY

414
G2455
136–153
YABBY

416
G2456
148–195
YABBY

418
G2457
110–127
YABBY

420
G2459
50–97
YABBY

422
G2467
25–118
HS

424
G2492
616–860
ENBP

426
G2505
9–137
NAC

428
G2515
1–57
MADS

430
G2525
196–308
DBP

432
G2536
5–135
NAC

434
G2543
31–91
HB

436
G2550
345–408
HB

438
G2559
60–170
DBP

440
G2565
243–292
GARP

442
G2567
18–384
ARF

444
G2570
235–283
GARP

446
G2571
133–200
AP2

448
G2574
225–284
WRKY

450
G2575
137–192
WRKY

452
G2579
52–119
AP2

454
G2585
103–162
WRKY

456
G2587
108–165
WRKY

458
G2592
119–429
TUBBY

460
G2597
62–200
TUBBY

462
G2603
104–389
TUBBY

464
G2604
34–64, 73–103
Z-LSDlike

466
G2616
79–139
HB

468
G2617
57–77
Z-C2H2

470
G2628
36–105
bZIP

472
G2632
170–221
CAAT

474
G2633
123–490
SCR

476
G2636
14–146
NAC

478
G2639
114–167
SRS

480
G2640
146–189
SRS

482
G2649
112–155
SRS

484
G2650
34–91
TEO

486
G2655
106–180
HLH/MYC

488
G2661
40–100
HLH/MYC

490
G2679
107–177
CPP

492
G2682
67–181
CPP

494
G2686
122–173
WRKY

496
G2690
46–113
AP2

498
G2691
78–145
AP2

500
G2694
1–446
OTHER

502
G2699
54–407
SCR

504
G2702
31–131
MYB-(R1)R2R3

506
G2717
5–58
MYB-related

508
G2718
21–76
MYB-related

510
G2723
12–174
MYB-related

512
G2741
140–205
GARP

514
G2743
201–249
GARP

516
G2747
19–113
ABI3/VP-1

518
G2754
198–393, 554–638
SWI/SNF

520
G2757
35–123, 348–434
TH

522
G2763
140–210
HLH/MYC

524
G2765
124–190
HLH/MYC

526
G2768
288–346
DBP

528
G2771
333–433
HLH/MYC

530
G2776
144–210
HLH/MYC

532
G2777
278–350
HLH/MYC

534
G2779
144–213
HLH/MYC

536
G2783
63–124, 151–235,
ACBF-like



262–318

538
G2784
139–260
DBP

540
G2790
137–200
HLH/MYC

542
G2802
48–196
NAC

544
G2805
2–169
NAC

546
G2826
75–95
Z-C2H2

548
G2830
245–266
Z-C2H2

550
G2832
11–31, 66–86, 317–337
Z-C2H2

552
G2834
246–266, 335–356
Z-C2H2

554
G2837
140–160
Z-C2H2

556
G2838
57–77
Z-C2H2

558
G2839
34–60, 85–113
Z-C2H2

560
G2846
266–329
HLH/MYC

562
G2847
205–268
HLH/MYC

564
G2850
318–381
HLH/MYC

566
G2851
248–309
HLH/MYC

568
G2854
110–250
ACBF-like

570
G2859
145–226
HLH/MYC

572
G2865
86–162
HLH/MYC

574
G2866
84–100, 139–168
IAA

576
G2869
26–409
ARF

578
G2884
228–276
GARP

580
G2885
196–243
GARP

582
G2887
4–180
NAC

584
G2888
41–61, 120–140
Z-C2H2

586
G2898
62–133
HMG

588
G2907
12–120, 854–923
PCGL

590
G2913
43–127
ARID

592
G2930
53–133
HLH/MYC

594
G2933
65–137
HLH/MYC

596
G2934
37–110
HLH/MYC

598
G2958
88–104, 143–172
IAA

600
G2964
41–63, 201–235
Z-C3H

602
G2967
66–88, 358–385
Z-C2H2

604
G2969
128–150
Z-C2H2

606
G2972
8–32, 129–149, 277–294
Z-C2H2

608
G2979
192–211
E2F

610
G2981
155–173
E2F

612
G2982
107–124
E2F

614
G2983
88–148
HB

616
G2990
54–109, 203–263
ZF–HB

618
G2992
29–84, 159–219
ZF–HB

620
G2993
85–138, 221–285
ZF–HB

622
G2996
75–126, 194–254
ZF–HB

624
G2998
74–127, 243–303
ZF–HB

626
G2999
82–131, 201–261
ZF–HB

628
G3002
6–50, 104–168
ZF–HB

630
G3003
131–280
Z-C2H2

632
G3008
10–275
EIL

634
G3017
133–201
HLH/MYC

636
G3021
110–155
HLH/MYC

638
G3032
285–333
GARP

640
G3044
222–311
HLH/MYC

642
G3054
77–96, 149–168
Z-C3H

644
G3055
97–115, 178–197,
Z-C3H



266–287

646
G3059
219–287
Z-C3H

648
G3060
42–61, 219–237
Z-C3H

650
G3061
73–90, 174–193
Z-C2H2

652
G3067
198–219
Z-C2H2

654
G3070
129–150
Z-C2H2

656
G3076
70–100, 182–209
bZIP–ZW2

658
G3083
75–105, 188–215
bZIP–ZW2

660
G3084
94–110, 148–177
IAA

662
G3086
297–376
HLH/MYC

664
G3091
34–131
PLATZ

666
G3094
7–143
PLATZ

668
G3095
16–151
PLATZ

670
G3111
111–152
RING/C3H2C3















































































































































































































































































































































































Examples of some of the utilities that may be desirable in plants, and that may be provided by transforming the plants with the presently disclosed sequences, are listed in Table 6. Many of the transcription factors listed in Table 6 may be operably linked with a specific promoter that causes the transcription factor to be expressed in response to environmental, tissue-specific or temporal signals. For example, G370 induces ectopic trichomes on flowers but also produces small plants. The former may be desirable to produce insect or herbivore resistance, or increased cotton yield, but the latter may be undesirable with respect to yield in that it may reduce biomass. However, by operably linking G370 with a flower-specific promoter, one may achieve the desirable benefits of the gene without affecting overall biomass to a significant degree. For examples of flower specific promoters, see Kaiser et al. (supra). For examples of other tissue-specific, temporal-specific or inducible promoters, see the above discussion under the heading “Vectors, Promoters, and Expression Systems”.
TABLE 6 Genes, traits and utilities that affect plant characteristics Trait Phenotypic Transcription factor Category alteration(s) genes that impact traits Utility Abiotic stress Effect of chilling on plants Increased sensitivity G2632; G2763; G2790; Improved growth G2885; G2993 rate, earlier Increased tolerance G1274; G1357; G1779; planting, yield G1928; G2063; G2567; G2579; G2650; G2771; G2930; G2933 Germination in cold Increased sensitivity G134; G344; G651; Temperature stress G1808; G2070; G2525; response manipulation G2543; G2592; G2993 Earlier planting; improved survival, yield Increased tolerance G224; G728; G807; G1274; G1837; G2051; G2317; G2603; G2784 Drought Increased tolerance G46; G47; G926; Improved survival, G975; G1206; G1274; vigor, appearance, G1357; G1452; G1792; yield, range G2133; G2505; G2717; G2982; G2999; G3076; G3086 Freezing G1206; G2982 Improved survival, vigor, appearance, yield Heat Increased sensitivity G550 Improved germination, Increased tolerance G3086 growth rate, later planting, yield Osmotic stress Increased sensitivity G155; G370 (KO); Abiotic stress response G1863; G1938; G2993; manipulation G2996 Increased tolerance G47; G353; G916; Improved germination G926; G1033; G1412; rate, survival, yield G1452; G1730; G1753; G1816; G2207; G2661; G2717; G2776; G2839; G2854; G2969 Salt tolerance Altered response (one line more G2394 tolerant, one line more sensitive) Increased tolerance G355; G624; G1017; Improved germination 1037; G1538; G1557; rate, survival, yield; G1660; G1837; G2035; extended growth range G2041; G2060; G2207; G2317; G2319; G2394; G2404; G2453; G2457; G2691; G2717; G2992; G2998; G2999; G3083; G3086 Nitrogen stress Sensitivity to N limitation G707; G1136; G1483; G1535; G1968; G1995; G2718; G2990; G2992 Less sensitive to N limitation G153; G200; G581; Improved yield and G839; G916; G1013; nutrient stress G1150; G1274; G1792; tolerance, decreased G1816; G1988; G2239; fertilizer usage G2604; G2718; G2830; G2913; G2981 Phosphate stress Sensitivity to PO 4 limitation G1995 Less sensitive to PO 4 limitation G355; G624; G1988; Improved yield and nutrient G2142; G2972 stress tolerance, decreased fertilizer usage Altered expression Induced by ABA G224; G244; G355; Modification of seed G571; G1037; G1482; development, seed G1507; G2070; G2085 dormancy, cold and dehydration tolerance Altered by auxin G151; G153; G224; Regulation of cell division, G244; G550; G807; growth and maturation, G1037; G1274; G1384; particularly at shoot tips G1482; G1535; G1923 Induced by salicylic acid G140; G224; G374; Resilience to heat or physio- G1037; G1100; G1274; logical conditions that G1507; G1538; G2070 result in high levels of salicylic acid After challenge with Erysiphe G314; G571; G1274; Yield, appearance, survival, G1923; G2070; G2085 extended range After challenge with Fusarium G140; G153; G171; Yield, appearance, survival, G224; G434; G1384; extended range G1507; G1923 Induced by heat G153; G171; G224; Germination, growth rate, G434; G550; G807; later planting G961; G1037; G1384; G1412; G1482; G1507; G1538; G1850; G1923; G2070 Cold G171; G224; G314; Improved growth rate, G1274; G1730; G2070; earlier planting, yield G2085; G2597 Osmotic stress G571; G1274; G1412; Abiotic stress response G1482; G1730; G1923; manipulation G2085 Drought G139; G244; G434; Improved survival, vigor, G571; G1100; G1412 appearance, yield Salt G224; G550; G1037 Improved germination rate, survival, yield; extended growth range Herbicide Glyphosate resistance G2133 Generation of glyphosate resistant plants, and increasing plant resistance to oxidative stress Hormone sensitivity Abscisic acid (ABA) sensitivity Reduced sensitivity or insensitive G12 (KO); G926; Modification of seed to ABA G1357; G1412; G1452; development, improved G1893; G2109; G2146; seed dormancy, cold and G2207; G2382; G2617; dehydration tolerance G2717; G2854; G2865; G2969; G2992; G3054; G3055; G3067 1-Aminocyclopropane-1- G12 Regulation of oxidative stress carboxylate (ACC) sensitivity and programmed cell death, Increased sensitivity to ACC, the delay over-ripening of fruit immediate precursor of ethylene Disease Botrytis Increased resistance or tolerance G1792 Improved yield, appearance, survival, extended range Fusarium Increased resistance or tolerance G1792 Improved yield, appearance, survival, extended range Erysiphe Increased resistance or tolerance G1792 Improved yield, appearance, survival, extended range Growth regulator Altered sugar sensing Decreased tolerance to sugars G155; G344; G478; Alteration of energy balance, G1420; G2111; G2763 photosynthetic rate, Increased tolerance to sugars G224; G905; 0916; carbohydrate accumulation, G1033; G1108; G1493; biomass production, source- G1535; G1753; G1816; sink relationships, senescence; G2661; G2776; G2839; alteration of storage G2854; G2898 compound accumulation in seeds Altered C/N sensing G153; G200; G581; Alteration or control of G707; G916; G1013; assimilate partitioning G1150; G1274; G1483; G1535; G1816; G1988; G2239; G2604; G2830; G2913; G2981 Flowering time Early flowering G129; G131; G135; Faster generation time; G136; G137; G138; synchrony of flowering; G140; G142; G145; additional harvests G146; G148; G153; within a growing season, G155; G172; G200; shortening of breeding G246; G416; G485 programs (OE); G549; G600; G627; G1011; G1037 (KO); G1142 (KO); G1538; G1797; G1798; G1823; G1825; G1988; G2071; G2129; G2142; G2184; G2311; G2372; G2443; G2515; G2628; G2633; G2639; G2650; G2754; G2777; G2779; G2802 (antisense clone); G2805; G2832; G2967; G2992; G3002; G3032; G3044; G3060; G3061; G3086 Late flowering G2; G15; G47; G173; Increased yield or biomass, G309; G319; G324; alleviate risk of transgenic G372; G380; G434; pollen escape, synchrony of G485 (KO); G571 flowering (KO); G581; G624; G707; G738; G744; G752; G839; G852; G905; G1113; G1136; G1142; G1150; G1276; G1357; G1361; G1446; G1451; G1452; G1468; G1474; G1493; G1549; G1554; G1863; G1945; G1983; G1998; G1999; G2106; G2146; G2207; G2251; G2269; G2319; G2334; G2432; G2559; G2604; G2694; G2723; G2741; G2743; G2763; G2771; G2802 (sense clone); G2838; G2846; G2964; G2979; G2993; G2998; G3003; G3021; G3060; G3111 Development and Altered flower structure morphology Stamen G15; G129; G133; Ornamental modification of G1420; G2455; G2694; plant architecture, improved G2768 or reduced fertility to miti- Sepal G129; G134; G140; gate escape of transgenic G1420; G2694; G2979; pollen, improved fruit size, G3094 shape, number or yield Petal G129; G133; G134; G140; G1420; G2768; G3094 Pedicel G1420; G1539; G1591; G2839; G2979; G2983 Carpel G129; G133; G446; G1539; G1591; G1796; G2455; G2579; G2617; G2694; G2768; G2983 Multiple alterations G15; G550; G651; G730; G1013; G1100; G1128; G1420; G1549; G1798; G1825; G1995; G2226; G2457; G2455; G2515; G2575; G2616; G2639; G2640; G2649; G2694; G2743; G2826; G2838; G2859; G2884; G3094 Changes in organ identity G129; G133; G134; G140 Enlarged floral organs G15; G2979 Increase in flower organ number G2768 ;G2979 Terminal flowers G1798; G2515 Flower organs persisting G1011; G1797 following fertilization Siliques G15; G2579; G2884 Broad, large rosettes G1274 Loss of flower determinacy G131; G135; G2768 Reduced fertility G15; G549; G651; G846; G1100; G1798; G2372; G2579; G2616; G2639; G2640; G2649; G2768; G2884 Gamete lethal G846 Altered shoot meristem G438 (KO); G916; development G1585; G1957; G2636; G2650; G2885 Inflorescence architectural Ornamental modification of change plant architecture, Altered inflorescence branching G47; G446; G2571; manipulation of growth and pattern G2146; G2571; G2694; development, increase leaf G2784; G2859 numbers, modulation of Short internodes/bushy G47; G253; G1274; branching patterns to provide inflorescences G1474; G1593; G1743; improved yield or biomass G1753; G1796; G2146; Ornamental modification of G2226; G2550; G2251; flower architecture; timing of G2575; G2616; G2639; flowering; altered plant habit G2640; G2649; G2958; for yield or harvestability G3021 benefit; reduction in pollen Terminal flowers G131; G135; G137; production of genetically G145; G148; G155; modified plants; manipulation G549; G1798; G2372; of seasonality and annual or G2515 perennial habit; manipulation Altered inflorescence G131; G135; G549; of determinate vs. determinacy G2372; G2515 indeterminate growth Aerial rosette development G1985; G1995; G2826; G2838 Downward pedicels G2839 Homeotic transformation G129, G133, G134; G140 Multiple inflorescence alterations G446; G549; G1798; G2616; G2694; G2784; G2839; G3059 Altered branching pattern G47; G438 (KO) Ornamental modification of plant architecture, improved lodging resistance Stern morphology and altered G47 Modulation of lignin content; vascular tissue structure improvement of wood, palatability of fruits and vegetables Apical dominance Reduced apical dominance G47 Ornamental modification of plant architecture; , improved lodging resistance Altered trichome density; development, or structure Ectopic trichomes G370; G2826 Ornamental modification of plant architecture, increased plant product (e.g., diterpenes, cotton) productivity, insect and herbivore resistance Altered trichome development G1539; G2983 Increased trichome number or G370; G1995; G2085; density G2826; G2838 Reduced or no trichomes G1452; G1816; G2718 Root development Decreased root growth or G651; G730; G2655; Modification of root secondary root development G2747; G2992; G2993 architecture and mass Decreased root branching G651; G2993 Influence uptake of water and nutrients Increased root branching G2747; G2992 Improved anchorage Abnormal gravitropic response G2983 Manipulation of root development Increased root hairs G1816; G2718; G2983 Improved yield, stress tolerance; anchorage Altered cotyledon shape G916; G1420; G1893; Ornamental applications G2432; G2636; G2859; G3059 Altered hypocotyl shape, color, G807; G916; G1510; Ornamental applications; development G1988; G2771; G2859; altered light response (see G2884; G2993 “Light Response”, below) Altered seed development, G961 Modification of seed ripening and germination germination properties and performance Slow growth G652; G1013; G1100; Ornamental applications G1468; G1535; G1549; G1779; G1938; G2765; G2784; G2826; G2834; G2851; G3091; G3095 Fast growth G807; G1476; G2617 Appearance, biomass, yield Cell differentiation and G1539; G1585; G1591; Increase in carpel or fruit cell proliferation G2885; G2983 development; improve regeneration of shoots from callus in transformation or micro-propagation systems Cell expansion G521 Control of cell elongation Phase change and floral reversion G370; G1985; G1995; Improved yield, biomass, G2826; G2838 manipulation of seasonality and annual or perennial habit, developmental plasticity in response to environmental stress Senescence Accelerated or premature G652; G1033; G1128; Improvement in response to senescence G1772; G2467; G2574; disease, fruit ripening G2783; G2907; G3059; G3111 Reduced or delayed senescence G571; G652 (KO); G2536 Abnormal embryo development G2884 Embryo lethal when knocked out G374 Herbicide target Gamete lethal G846 Potential to prevent escape of GMO pollen Altered programmed cell death G12 Lethality when overexpressed G366; G1384; G1556; Herbicide target; ablation of G1832; G1850; G1957; specific tissues or organs such G1990; G2213; G2298; as stamen to prevent pollen G2505; G2570; G2587; escape G2869; G2887 Necrosis, formation of necrotic G12; G1840 Disease resistance lesions Plant size Increased plant size or biomass G46; G268; G287; Improved yield, biomass, G314; G319; G324; appearance G438; G624; G852; G1113; G1150; G1451; G1468; G2334; G2536; G2650; G2741; G2979 Large seedlings G1313; G2679; G2694; Increased survival and vigor G2838 of seedlings, yield Dwarfed or more G131; G136; G253; Dwarfism, lodging resistance, compact plants G309; G370; G386; manipulation of gibberellin G549; G550; G600; responses G651; G652; G707; G738; G811; G1011; G1100; G1247; G1289; G1340; G1423; G1474; G1483; G1549; G1554; G1593; G1753; G1772; G1779; G1798; G1938; G1983; G1993; G2085; G2226; G2227; G2251; G2372; G2375; G2453; G2456; G2459; G2492; G2515; G2550; G2565; G2574; G2575; G2579; G2616; G2628; G2640; G2649; G2682; G2702; G2757; G2783; G2839; G2846; G2847; G2850; G2884; G2934; G2958; G2979; G2992; G3017; G3059; G3091; G3111 Leaf morphology Dark green leaves G30; G253; G309; Increased photosynthesis, G707; G811; G957; biomass, appearance, yield; G1100; G1128; G1327; nutritional value G1341; G1357; G1389; G1420; g1423; G1452; G1482; G1510; G1535; G1549; G1554; G1593; G1743; G1792; G1796; G1846; G1863; G1932; G1938; G1983; G2085; G2146; G2207; G2226; G2251; G2334; G2371; G2372; G2453; G2456; G2457; G2459; G2550; G2640; G2649; G2661; G2690; G2694; G2771; G2763; G2784; G2837; G2838; G2846; G2847; G2850; G2851; G2958; G2993; G3021; G3059; G3091; G3095; G3111 Change in leaf shape G30; G129; G131; Ornamental applications G135; G136; G137; G140; G148; G200; G224; G253; G319; G370; G372; G438; G446; G468; G600; G646; G651; G707; G905; G957; G1011; G1013; G1100; G1113; G1128; G1142; G1247; G1341; G1357; G1361; G1389; G1420; G1452; G1468; G1474; G1476; G1493; G1535; G1538; G1549; G1557; G1585; G1593; G1743; G1796; G1798; G1825; G1846; G1863; G1893; G1917; G1932; G1938; G1945; G1983; G1993; G2084; G2085; G2207; G2226; G2227; G2251; G2334; G2375; G2432; G2453; G2455; G2456; G2457; G2536; G2550; G2565; G2575; G2579; G2604; G2617; G2628; G2636; G2639; G2640; G2649; G2682; G2686; G2690; G2694; G2699; G2702; G2747; G2768; G2771; G2784; G2837; G2839; G2846; G2850; G2851; G2859; G2866; G2888; G2958; G2992; G3021; G3044; G3059; G3084; G3091; G3094; G3095; G3111 Increased leaf size and mass G268; G324; G438; Increased yield, ornamental G852; G1113; G1274; applications G1451; G2536; G2699; G2768; G3008 Light green or gray leaves G351; G600; G651; Ornamental applications G1468; G1718; 02565; G2604; G2779; 02859; G3044; G3070 Glossy leaves G30; G370 (KO); Ornamental applications, G975; G1792; G2640; manipulation of wax G2649 composition, amount, or distribution Altered abaxial/adaxial polarity G730 Modification of plant growth and form Seed morphology Altered seed coloration G581; G961; G2085; Appearance G2371 Seed size and shape Altered seed shape G652; G916; G961 Appearance Large seed G151; G581; G2085; G2585; G2933 Leaf biochemistry Increased leaf wax G975 Insect, pathogen resistance Leaf fatty acids G975 Increase in leaf fatty acids Seed biochemistry Seed oil content Increased oil content G961 (KO); G1451 Improved oil yield, increased (KO); G2830 (KO) caloric content of food and animal feed Seed prenyl lipids G652 (KO) Increase in alpha-tocopherol (vitamin E) Light Altered cotyledon G30; G2754; G2859 Increased planting densities response/shade and yield enhancement avoidance Altered hypocotyl G30; G807; G916; G1082; G1510; G1988; G2650; G2754; G2771; G2859; G2884; G2993 Altered leaf orientation G351; G1013; G2650; G2694; G2993; G3032 Altered petiole G478; G807; G1988; G2650; G2694; G2754 Shade avoidance G30; G246; G353; G354; G2432; G2650; G2754 Pigment Increased anthocyanin levels G253; G386; G707; Enhanced health benefits, G1482; G2453; G2456; improved ornamental G2459 appearance, increased stress resistance, attraction of pollinating and seed- dispersing animals Decreased anthocyanin levels G581; G2604; G2718 Abbreviations: N = nitrogen P = phosphate ABA = abscisic acid C/N = carbon/nitrogen balance
Detailed Description of Genes, Traits and Utilities that Affect Plant Characteristics

The following descriptions of traits and utilities associated with the present transcription factors offer a more comprehensive description than that provided in Table 6.
Abiotic Stress, General Considerations
Plant transcription factors can modulate gene expression, and, in turn, be modulated by the environmental experience of a plant. Significant alterations in a plant's environment invariably result in a change in the plant's transcription factor gene expression pattern. Altered transcription factor expression patterns generally result in phenotypic changes in the plant. Transcription factor gene product(s) in transgenic plants then differ(s) in amounts or proportions from that found in wild-type or non-transformed plants, and those transcription factors likely represent polypeptides that are used to alter the response to the environmental change. By way of example, it is well accepted in the art that analytical methods based on altered expression patterns may be used to screen for phenotypic changes in a plant far more effectively than can be achieved using traditional methods.
Abiotic stress: adult stage chilling. Enhanced chilling tolerance produced by modifying expression levels of transcription factors such as G1274, G1357, G1779, G1928, G2063, G2567, G2579, G2650, G2771, G2930, or G2933, for example, in plants may extend the effective growth range of chilling sensitive crop species by allowing earlier planting or later harvest. Improved chilling tolerance may be conferred by increased expression of glycerol-3-phosphate acetyltransferase in chloroplasts (see, for example, Wolter et al. (1992) EMBO J. 4685–4692, and Murata et al. (1992) Nature 356: 710–713).
Chilling tolerance could also serve as a model for understanding how plants adapt to water deficit. Both chilling and water stress share similar signal transduction pathways and tolerance/adaptation mechanisms. For example, acclimation to chilling temperatures can be induced by water stress or treatment with abscisic acid. Genes induced by low temperature include dehydrins (or LEA proteins). Dehydrins are also induced by salinity, abscisic acid, water stress and during the late stages of embryogenesis.
Another large impact of chilling occurs during post-harvest storage. For example, some fruits and vegetables do not store well at low temperatures (for example, bananas, avocados, melons, and tomatoes). The normal ripening process of the tomato is impaired if it is exposed to cool temperatures. Genes conferring resistance to chilling temperatures may enhance tolerance during post-harvest storage.
Abiotic stress: cold germination. The potential utility of presently disclosed transcription factor genes that increase tolerance to cold is to confer better germination and growth in cold conditions. Plants with modified expression levels of G224, G728, G807, G1274, G11837, G2051, G2317, G2603, or G2784 show less sensitivity to germination in cold conditions, indicating a role in regulation of cold responses. These genes might be engineered to manipulate the response to low temperature stress. Genes that would allow germination and seedling vigor in the cold would have highly significant utility in allowing seeds to be planted earlier in the season with a high rate of survival. Transcription factor genes that confer better survival in cooler climates allow a grower to move up planting time in the spring and extend the growing season further into autumn for higher crop yields. Germination of seeds and survival at temperatures significantly below that of the mean temperature required for germination of seeds and survival of non-transformed plants would increase the potential range of a crop plant into regions in which it would otherwise fail to thrive.
Abiotic Stress: Salt and Drought Tolerance
Plants are subject to a range of environmental challenges. Several of these, including salt stress, general osmotic stress, drought stress and freezing stress, have the ability to impact whole plant and cellular water availability. Not surprisingly, then, plant responses to this collection of stresses are related. In a recent review, Zhu notes that (Zhu (2002) Ann. Rev. Plant Biol. 53: 247–273) “most studies on water stress signaling have focused on salt stress primarily because plant responses to salt and drought are closely related and the mechanisms overlap”. Many examples of similar responses (i.e., genetic pathways to this set of stresses have been documented. For example, the CBF transcription factors have been shown to condition resistance to salt, freezing and drought (Kasuga et al. (1999) Nature Biotech. 17: 287–291). The Arabidopsis rd29B gene is induced in response to both salt and dehydration stress, a process that is mediated largely through an ABA signal transduction process (Uno et al. (2000) Proc. Natl. Acad. Sci . USA 97: 11632–11637), resulting in altered activity of transcription factors that bind to an upstream element within the rd29B promoter. In Mesembryanthemum crystallinum (ice plant), Patharker and Cushman have shown that a calcium-dependent protein kinase (McCDPK1) is induced by exposure to both drought and salt stresses (Patharker and Cushman (2000) Plant J. 24: 679–691). The stress-induced kinase was also shown to phosphorylate a transcription factor, presumably altering its activity, although transcript levels of the target transcription factor are not altered in response to salt or drought stress. Similarly, Saijo et al. demonstrated that a rice salt/drought-induced calmodulin-dependent protein kinase (OsCDPK7) conferred increased salt and drought tolerance to rice when overexpressed (Saijo et al. (2000) Plant J. 23: 319–327).
Exposure to dehydration invokes similar survival strategies in plants as does freezing stress (see, for example, Yelenosky (1989) Plant Physiol 89: 444–451) and drought stress induces freezing tolerance (see, for example, Siminovitch et al. (1982) Plant Physiol 69: 250–255; and Guy et al. (1992) Planta 188: 265–270). In addition to the induction of cold-acclimation proteins, strategies that allow plants to survive in low water conditions may include, for example, reduced surface area, or surface oil or wax production.
Consequently, one skilled in the art would expect that some pathways involved in resistance to one of these stresses, and hence regulated by an individual transcription factor, will also be involved in resistance to another of these stresses, regulated by the same or homologous transcription factors. Of course, the overall resistance pathways are related, not identical, and therefore not all transcription factors controlling resistance to one stress will control resistance to the other stresses. Nonetheless, if a transcription factor conditions resistance to one of these stresses, it would be apparent to one skilled in the art to test for resistance to these related stresses. Modifying the expression of a number of presently disclosed transcription factor genes shown to confer increased tolerance to drought, e.g., G46, G47, G926, G975, G1206, G1274, G1357, G1452, G1792, G2133, G2505, G2717, G2982, G2999, G3076, and G3086, and increased tolerance to salt, e.g., G355, G624, G1017, G1037, G1538, G1557, G1660, G1837, G2035, G2041, G2060, G2207, G2317, G2319, G2404, G2453, G2457, G2691, G2717, G2992, G2998, G2999, G3083, and G3086, during germination, the seedling stage, and throughout a plant's life cycle, may thus be used to increase a plant's tolerance to low water conditions and provide the benefits of improved survival, increased yield and an extended geographic and temporal planting range.
Abiotic stress: freezing tolerance and osmotic stress. Modification of the expression of a number of presently disclosed transcription factor genes, G47, G353, G916, G926, G1033, G1206, G1412, G1452, G1730, G1753, G1816, G2207, G2661, G2717, G2776, G2839, G2854, G2969, or G2982, for example, may be used to increase germination rate or growth under adverse osmotic conditions, which could impact survival and yield of seeds and plants. Osmotic stresses may be regulated by specific molecular control mechanisms that include genes controlling water and ion movements, functional and structural stress-induced proteins, signal perception and transduction, and free radical scavenging, and many others (Wang et al. (2001) Acta Hort . (ISHS) 560: 285–292). Instigators of osmotic stress include freezing, drought and high salinity, each of which are discussed in more detail below.
In many ways, freezing, high salt and drought have similar effects on plants, not the least of which is the induction of common polypeptides that respond to these different stresses. For example, freezing is similar to water deficit in that freezing reduces the amount of water available to a plant. Exposure to freezing temperatures may lead to cellular dehydration as water leaves cells and forms ice crystals in intercellular spaces (Buchanan, supra). As with high salt concentration and freezing, the problems for plants caused by low water availability include mechanical stresses caused by the withdrawal of cellular water. Thus, the incorporation of transcription factors that modify a plant's response to osmotic stress into, for example, a crop or ornamental plant, may be useful in reducing damage or loss. Specific effects caused by freezing, high salt and drought are addressed below.
Abiotic stress: heat stress tolerance. The germination of many crops is also sensitive to high temperatures. Presently disclosed transcription factor genes, including, for example, G3086, that provide increased heat tolerance, are generally useful in producing plants that germinate and grow in hot conditions, may find particular use for crops that are planted late in the season, or extend the range of a plant by allowing growth in relatively hot climates.
Nutrient uptake and utilization: nitrogen and phosphorus. Presently disclosed transcription factor genes introduced into plants provide a means to improve uptake of essential nutrients, including nitrogenous compounds, phosphates, potassium, and trace minerals. The enhanced performance of, for example, G153, G200, G581, G839, G916, G1013, G150, G1274, G1792, G1816, G1988, G2239, G2604, G2718, G2830, G2913, and G2981, and other overexpressing lines under low nitrogen conditions or G355, G624, G1988, G2142, and G2972 under low phosphorus conditions indicate that these genes and their homologs could be used to engineer crops that could thrive under conditions of reduced nutrient availability. Phosphorus, in particular, tends to be a limiting nutrient in soils and is generally added as a component in fertilizers. Young plants have a rapid intake of phosphate and sufficient phosphate is important for yield of root crops such as carrot, potato and parsnip.
The effect of these modifications is to increase the seedling germination and range of ornamental and crop plants. The utilities of presently disclosed transcription factor genes conferring tolerance to conditions of low nutrients also include cost savings to the grower by reducing the amounts of fertilizer needed, environmental benefits of reduced fertilizer runoff into watersheds; and improved yield and stress tolerance. In addition, by providing improved nitrogen uptake capability, these genes can be used to alter seed protein amounts and/or composition in such a way that could impact yield as well as the nutritional value and production of various food products.
Decreased herbicide sensitivity. Presently disclosed transcription factor genes, including G2133 and its equivalogs that confer resistance or tolerance to herbicides (e.g., glyphosate) will find use in providing means to increase herbicide applications without detriment to desirable plants. This would allow for the increased use of a particular herbicide in a local environment, with the effect of increased detriment to undesirable species and less harm to transgenic, desirable cultivars.
Knockouts of a number of the presently disclosed transcription factor genes have been shown to be lethal to developing embryos. Thus, these genes are potentially useful as herbicide targets.
Altered expression and hormone sensitivity: abscisic acid and auxin. Altering the expression levels of a number of the presently disclosed transcription factor genes, including G12, G224, G244, G355, G571, G926, G1037, G1357, G1412, G1452, G1482, G1507, G1893, G2070, G2085, G2109, G2146, G2207, G2382, G2617, G2717, G2854, G2865, G2969, G2992, G3054, G3055, or G3067, may be used to reduce a plant's sensitivity to ABA or render a plant insensitive to ABA exposure. ABA plays regulatory roles in a host of physiological processes in all higher as well as in lower plants (Davies et al. (1991) Abscisic Acid: Physiology and Biochemistry . Bios Scientific Publishers, Oxford, UK; Zeevaart et al. (1988) Ann. Rev. Plant Physiol . Plant Mol. Biol. 49: 439–473; Shimizu-Sato et al. (2001) Plant Physiol 127: 1405–1413). ABA mediates stress tolerance responses in higher plants, is a key signal compound that regulates stomatal aperture and, in concert with other plant signaling compounds, is implicated in mediating responses to pathogens and wounding or oxidative damage (for example, see Larkindale et al. (2002) Plant Physiol. 128: 682–695). In seeds, ABA promotes seed development, embryo maturation, synthesis of storage products (proteins and lipids), desiccation tolerance, and is involved in maintenance of dormancy (inhibition of germination), and apoptosis (Zeevaart et al. (1988) Ann. Rev. Plant Physiol. Plant Mol. Biol. 49: 439–473; Davies (1991), supra; Thomas (1993) Plant Cell 5: 1401–1410; and Bethke et al. (1999) Plant Cell 11: 1033–1046). ABA also affects plant architecture, including root growth and morphology and root-to-shoot ratios. ABA action and metabolism is modulated not only by environmental signals but also by endogenous signals generated by metabolic feedback, transport, hormonal cross-talk and developmental stage. Manipulation of ABA levels, and hence by extension the sensitivity to ABA, has been described as a very promising means to improve productivity, performance and architecture in plants Zeevaart (1999) in: Biochemistry and Molecular Biology of Plant Hormones , Hooykaas et al. eds, Elsevier Science pp 189–207; and Cutler et al. (1999) Trends Plant Sci. 4: 472–478).
A number of genes have been shown to be induced by cold acclimation in higher plants, including, for example, G171, G224, G1274, G1730, G2085, and G2597, and the proteins encoded by these genes are thought to play a role in protecting plant cells from injury, including freezing (Nagao et al. (2002) Plant Cell Physiol. 43: S168–S168). Since ABA mediates conversion of apical meristems into dormant buds, altered expression to ABA may increase protection of the buds from mechanical damage during winter. A plant's response to ABA also affects sprouting inhibition during premature warm spells. ABA is also important in protecting plants from drought tolerance. Thus, by affecting ABA sensitivity, introduced transcription factor genes may affect cold sensitivity, yield and survival, and plants with G12 knocked-out or plants overexpressing G926, G1357, G1412, G1452, G1893, G2109, G2146, G2207, G2382, G2617, G2717, G2854, G2865, G2969, G2992, G3054, G3055, and G3067, may have modified ABA responses that influence seed development and dormancy, as well as cold and dehydration tolerance, and survival.
“Auxin” refers to a class of plant hormones, including indoleacetic acid (IAA), having a variety of effects, such as phototropic response through the stimulation of cell elongation, stimulation of secondary growth, and the development of leaf traces and fruit. Specifically, auxin is involved in the regulation of cell division, particularly at shoot tips. Transcription factors genes that regulate a plant's response to auxin thus provide a means for controlling shoot tip development and secondary growth, which in turn can be used to manipulate plant growth and development.
Disease resistance or tolerance: Erysiphe, Fusarium, Botrytis , and other pathogens. A number of the presently disclosed transcription factor genes have been induced to be expressed (e.g., G140, G171, G224, G434, G571, G1100, G1274, G1384, G1507, G1538, G1923, and G2085), or have been shown to provide resistance or tolerance (e.g., G1792) after challenge with more than one pathogen, including fungal pathogens Fusarium oxysporum, Botrytis cinerea and Erysiphe orontii . Modification of the expression levels of one or more transcription factor genes may provide some benefit to the plant to help prevent or overcome infestation. The mechanisms by which the transcription factors work could include changing surface characteristics such as waxes, oils, or cell wall composition and thickness, or by the activation of signal transduction pathways that regulate plant defenses in response to attacks by pathogens (including, for example, reactive oxygen species, anti-fungal proteins, defensins, thionins, glucanases, and chitinases). Another means to combat fungal and other pathogens is by accelerating local cell death or senescence, mechanisms used to impair the spread of pathogenic microorganisms throughout a plant. For instance, the best known example of accelerated cell death is the resistance gene-mediated hypersensitive response, which causes localized cell death at an infection site and initiates a systemic defense response. Because many defenses, signaling molecules, and signal transduction pathways are common to defense against different pathogens and pests, such as fungal, bacterial, oomycete, nematode, and insect, transcription factors that are implicated in defense responses against the fungal pathogens tested may also function in defense against other pathogens and pests.
Growth Regulator: Sugar Sensing.
In addition to their important role as an energy source and structural component of the plant cell, sugars are central regulatory molecules that control several aspects of plant physiology, metabolism and development (Hsieh et al. (1998) Proc. Natl. Acad. Sci. 95: 13965–13970). It is thought that this control is achieved by regulating gene expression and, in higher plants, sugars have been shown to repress or activate plant genes involved in many essential processes such as photosynthesis, glyoxylate metabolism, respiration, starch and sucrose synthesis and degradation, pathogen response, wounding response, cell cycle regulation, pigmentation, flowering and senescence. The mechanisms by which sugars control gene expression are not understood.
Several sugar-sensing mutants have turned out to be allelic to abscisic acid (ABA) and ethylene mutants. ABA is found in all photosynthetic organisms and acts as a key regulator of transpiration, stress responses, embryogenesis, and seed germination. Most ABA effects are related to the compound acting as a signal of decreased water availability, whereby it triggers a reduction in water loss, slows growth, and mediates adaptive responses. However, ABA also influences plant growth and development via interactions with other phytohormones. Physiological and molecular studies indicate that maize and Arabidopsis have almost identical pathways with regard to ABA biosynthesis and signal transduction. For further review, see Finkelstein and Rock ((2002) Abscisic acid biosynthesis and response (In The Arabidopsis Book , Editors: Somerville and Meyerowitz (American Society of Plant Biologists, Rockville, Md.).
This potentially implicates G155, G224, G344, G478, G905, G916, G1033, G1108, G1420, G1493, G1535, G1753, G1816, G2111, G2661, G2763, G2776, G2839, G2854, G2898 and related transcription factors in hormone signaling based on the sucrose sugar sensing phenotype of transgenic lines overexpressing these polypeptides. On the other hand, the sucrose treatment used in these experiments (9.5% w/v) could also be an osmotic stress. Therefore, one could interpret these data as an indication that these transgenic lines overexpressing are more tolerant to osmotic stress. However, it is well known that plant responses to ABA, osmotic and other stress may be linked, and these different treatments may even act in a synergistic manner to increase the degree of a response. For example, Xiong, Ishitani, and Zhu ((1999) Plant Physiol. 119: 205–212) have shown that genetic and molecular studies may be used to show extensive interaction between osmotic stress, temperature stress, and ABA responses in plants. These investigators analyzed the expression of RD29A-LUC in response to various treatment regimes in Arabidopsis . The RD29A promoter contains both the ABA-responsive and the dehydration-responsive element—also termed the C-repeat—and can be activated by osmotic stress, low temperature, or ABA treatment; transcription of the RD29A gene in response to osmotic and cold stresses is mediated by both ABA-dependent and ABA-independent pathways (Xiong, Ishitani, and Zhu (1999) supra). LUC refers to the firefly luciferase coding sequence, which, in this case, was driven by the stress responsive RD29A promoter. The results revealed both positive and negative interactions, depending on the nature and duration of the treatments. Low temperature stress was found to impair osmotic signaling but moderate heat stress strongly enhanced osmotic stress induction, thus acting synergistically with osmotic signaling pathways. In this study, the authors reported that osmotic stress and ABA could act synergistically by showing that the treatments simultaneously induced transgene and endogenous gene expression. Similar results were reported by Bostock and Quatrano ((1992) Plant Physiol. 98: 1356–1363), who found that osmotic stress and ABA act synergistically and induce maize Em gene expression. Ishitani et al (1997) Plant Cell 9: 1935–1949) isolated a group of Arabidopsis single-gene mutations that confer enhanced responses to both osmotic stress and ABA. The nature of the recovery of these mutants from osmotic stress and ABA treatment indicated that although separate signaling pathways exist for osmotic stress and ABA, the pathways share a number of components; these common components may mediate synergistic interactions between osmotic stress and ABA. Thus, contrary to the previously held belief that ABA-dependent and ABA-independent stress signaling pathways act in a parallel manner, our data reveal that these pathways cross talk and converge to activate stress gene expression.
Because sugars are important signaling molecules, the ability to control either the concentration of a signaling sugar or how the plant perceives or responds to a signaling sugar could be used to control plant development, physiology or metabolism. For example, the flux of sucrose (a disaccharide sugar used for systemically transporting carbon and energy in most plants) has been shown to affect gene expression and alter storage compound accumulation in seeds. Manipulation of the sucrose-signaling pathway in seeds may therefore cause seeds to have more protein, oil or carbohydrate, depending on the type of manipulation. Similarly, in tubers, sucrose is converted to starch which is used as an energy store. It is thought that sugar signaling pathways may partially determine the levels of starch synthesized in the tubers. The manipulation of sugar signaling in tubers could lead to tubers with a higher starch content.
Thus, the presently disclosed transcription factor genes that manipulate the sugar signal transduction pathway may lead to altered gene expression to produce plants with desirable traits. In particular, manipulation of sugar signal transduction pathways could be used to alter source-sink relationships in seeds, tubers, roots and other storage organs leading to increase in yield.
Growth regulator: carbon and nitrogen balance. A number of the transcription factor-overexpressing lines, including G153, G200, G581, G707, G916, G1013, G1150, G1274, G1483, G1535, G1816, G1988, G2239, G2604, G2830, G2913, and G2981, may be used to produce plants with altered C/N sensing. These plants may, for example, make less anthocyanin on high sucrose plus glutamine, indicating that these genes can be used to modify carbon and nitrogen status, and hence assimilate partitioning (assimilate partitioning refers to the manner in which an essential element, such as nitrogen, is distributed among different pools inside a plant, generally in a reduced form, for the purpose of transport to various tissues).
Flowering time: early and late flowering. Presently disclosed transcription factor genes that accelerate flowering, which include G129, G131, G135, G136, G137, G138, G140, G142, G145, G146, G148, G153, G155, G172, G200, G246, G416, G485, G549, G600, G627, G1011, G1037, G1142, G1538, G1797, G1798, G1823, G1825, G1988, G2071, G2129, G2142, G2184, G2311, G2372, G2443, G2515, G2628, G2633, G2639, G2650, G2754, G2777, G2779, G2802, G2805, G2832, G2967, G2992, G3002, G3032, G3044, G3060, G3061, and G3086, could have valuable applications in such programs, since they allow much faster generation times. In a number of species, for example, broccoli, cauliflower, where the reproductive parts of the plants constitute the crop and the vegetative tissues are discarded, it would be advantageous to accelerate time to flowering. Accelerating flowering could shorten crop and tree breeding programs. Additionally, in some instances, a faster generation time would allow additional harvests of a crop to be made within a given growing season. A number of Arabidopsis genes have already been shown to accelerate flowering when constitutively expressed. These include LEAFY, APETALA1 and CONSTANS (Mandel et al. (1995) Nature 377: 522–524; Weigel and Nilsson (1995) Nature 377: 495–500; Simon et al. (1996) Nature 384: 59–62).
By regulating the expression of potential flowering using inducible promoters, flowering could be triggered by application of an inducer chemical. This would allow flowering to be synchronized across a crop and facilitate more efficient harvesting. Such inducible systems could also be used to tune the flowering of crop varieties to different latitudes. At present, species such as soybean and cotton are available as a series of maturity groups that are suitable for different latitudes on the basis of their flowering time (which is governed by day-length). A system in which flowering could be chemically controlled would allow a single high-yielding northern maturity group to be grown at any latitude. In southern regions such plants could be grown for longer periods before flowering was induced, thereby increasing yields. In more northern areas, the induction would be used to ensure that the crop flowers prior to the first winter frosts.
In a sizeable number of species, for example, root crops, where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it is advantageous to identify and incorporate transcription factor genes that delay or prevent flowering in order to prevent resources being diverted into reproductive development. For example, G2, G15, G47, G173, G309, G319, G324, G372, G380, G434, G485, G571, G581, G624, G707, G738, G744, G752, G839, G852, G905, G1113, G1136, G1142, G1150, G1276, G1357, G1361, G1446, G1451, G1452, G1468, G1474, G1493, G1549, G1554, G1863, G1945, G1983, G1998, G1999, G2106, G2146, G2207, G2251, G2269, G2319, G2334, G2432, G2559, G2604, G2694, G2723, G2741, G2743, G2763, G2771, G2802, G2838, G2846, G2964, G2979, G2993, G2998, G3003, G3021, G3060, and G3111 have been shown to delay flowering time in plants. Extending vegetative development with presently disclosed transcription factor genes could thus bring about large increases in yields. Prevention of flowering can help maximize vegetative yields and prevent escape of genetically modified organism (GMO) pollen.
Presently disclosed transcription factors that extend flowering time have utility in engineering plants with longer-lasting flowers for the horticulture industry, and for extending the time in which the plant is fertile.
Altered flower structure and inflorescence: aerial rosettes architecture, branching, short internodes, terminal flowers and phase change. Presently disclosed transgenic transcription factors such as G15, G129, G131, G133, G134, G135, G140, G446, G549, G550, G651, G730, G846, G1011, G1013, G1100, G1128, G1274, G1420, G1539, G1549, G1591, G1796, G1797, G1798, G1825, G1995, G2226, G2372, G2455, G2457, G2515, G2575, G2579, G2616, G2617, G2639, G2640, G2649, G2694, G2743, G2768, G2826, G2838, G2839, G2859, G2884, G2979, G2983, and G3094 have been used to create plants with larger flowers or arrangements of flowers that are distinct from wild-type or non-transformed cultivars. This would likely have the most value for the ornamental horticulture industry, where larger flowers or interesting floral configurations are generally preferred and command the highest prices.
Flower structure may have advantageous or deleterious effects on fertility, and could be used, for example, to decrease fertility by the absence, reduction or screening of reproductive components. In fact, plants that overexpress a sizable number of the presently disclosed transcription factor genes, including G15, G549, G651, G846, G1100, G1798, G2372, G2579, G2616, G2639, G2640, G2649, G2768, and G2884, have been shown to possess reduced fertility compared with control plants. These could be desirable traits, as low fertility could be exploited to prevent or minimize the escape of the pollen of genetically modified organisms (GMOs) into the environment.
The alterations in shoot architecture seen in the lines in which the expression G47, G446, G2571, G2146, G2571, G2694, G2784, or G2859, for example, was modified indicates that these genes can be used to manipulate inflorescence branching patterns. This could influence yield and offer the potential for more effective harvesting techniques. For example, a “self pruning” mutation of tomato results in a determinate growth pattern and facilitates mechanical harvesting (Pnueli et al. (2001) Plant Cell 13(12): 2687–702).
Although the fertility of plants overexpressing some of the lines in which the present transcription factors (e.g., G2579) expression levels were poor, siliques of these plants appeared to grow out fairly extensively in many instances, indication that these genes may be producing parthenocarpic effects (fruit development in the absence of seed set), and may have utility in producing seedless fruit.
One interesting application for manipulation of flower structure, for example, by introduced transcription factors could be in the increased production of edible flowers or flower parts, including saffron, which is derived from the stigmas of Crocus sativus.
A number of the presently disclosed transcription factors may affect the timing of phase changes in plants (e.g., G370, G1985, G1995, G2826, and G2838). Since the timing or phase changes generally affects a plant's eventual size, these genes may prove beneficial by providing means for improving yield and biomass.
General development and morphology: shoot meristem and branching patterns. Presently disclosed transcription factor genes, when introduced into plants, may be used to modify branching patterns (e.g., by knocking-out G438, and overexpression of G916, G1585, G1957, G2636, G2650, and G2885), for example, by causing stem bifurcations in developing shoots in which the shoot meristems split to form two or three separate shoots. These transcription factors and their functional equivalogs may thus be used to manipulate branching. This would provide a unique appearance, which may be desirable in ornamental applications, and may be used to modify lateral branching for use in the forestry industry. A reduction in the formation of lateral branches could reduce knot formation. Conversely, increasing the number of lateral branches could provide utility when a plant is used as a view- or windscreen. Transcription factors that cause primary shoots to become ‘kinked’ at each coflorescence node (e.g., G47) may be used to manipulate plant structure and provide for a unique ornamental appearance.
General development and morphology: apical dominance: The modified expression of presently disclosed transcription factors (e.g., G47, and its equivalogs) that reduce apical dominance could be used in ornamental horticulture, for example, to modify plant architecture, for example, to produce a shorter, more bushy stature than wild type. The latter form would have ornamental utility as well as provide increased resistance to lodging.
Development and morphology: trichomes. Several of the presently disclosed transcription factor genes have been used to modify trichome number, density, trichome cell fate or amount of trichome products produced by plants. These include G370, G1452, G1539, G1816, G1995, G2085, G2718, G2826, G2838, and G2983. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity. Thus, by increasing trichome density, size or type, trichome-affecting genes and their homologs would have profound utilities in molecular farming practices and increasing the yield of cotton fibers.
If the effects on trichome patterning reflect a general change in heterochronic processes, trichome-affecting transcription factors or their homologs can be used to modify the way meristems and/or cells develop during different phases of the plant life cycle. In particular, altering the timing of phase changes could afford positive effects on yield and biomass production.
General development and morphology: stem morphology and altered vascular tissue structure. Plants in which expression of transcription factor gene that modify stem morphology or lignin content is modified may be used to affect overall plant architecture and the distribution of lignified fiber cells within the stem.
Modulating lignin content might allow the quality of wood used for furniture or construction to be improved. Lignin is energy rich; increasing lignin composition could therefore be valuable in raising the energy content of wood used for fuel. Conversely, the pulp and paper industries seek wood with a reduced lignin content. Currently, lignin must be removed in a costly process that involves the use of many polluting chemicals. Consequently, lignin is a serious barrier to efficient pulp and paper production (Tzfira et al. (1998) TIBTECH 16: 439–446; Robinson (1999) Nature Biotechnology 17: 27–30). In addition to forest biotechnology applications, changing lignin content by selectively expressing or repressing transcription factors in fruits and vegetables might increase their palatability.
Transcription factors that modify stem structure, including G47 and its equivalogs, may also be used to achieve reduction of higher-order shoot development, resulting in significant plant architecture modification. Overexpression of the genes that encode these transcription factors in woody plants might result in trees that lack side branches, and have fewer knots in the wood. Altering branching patterns could also have applications amongst ornamental and agricultural crops. For example, applications might exist in any species where secondary shoots currently have to be removed manually, or where changes in branching pattern could increase yield or facilitate more efficient harvesting.
General development and morphology: altered root development. By modifying the structure or development of roots by modifying expression levels of one or more of the presently disclosed transcription factor genes, including G651, G730, G1816, G2655, G2718, G2747, G2983, G2992, G2993, and their equivalogs, plants may be produced that have the capacity to thrive in otherwise unproductive soils. For example, grape roots extending further into rocky soils would provide greater anchorage, greater coverage with increased branching, or would remain viable in waterlogged soils, thus increasing the effective planting range of the crop and/or increasing yield and survival. It may be advantageous to manipulate a plant to produce short roots, as when a soil in which the plant will be growing is occasionally flooded, or when pathogenic fungi or disease-causing nematodes are prevalent.
In addition, presently disclosed transcription factors including G1816, G2718, G2983 and their equivalogs, may be used to increase root hair density and thus increase tolerance to abiotic stresses, thereby improving yield and quality.
Development and morphology: cotyledon, hypocotyl. The morphological phenotypes shown by plants overexpressing several of the transcription factor genes in Table 6 indicate that these genes, including those that produce altered cotyledons (e.g., G916, G1420, G1893, G2432, G2636, G2859, and G3059) and hypocotyls (G807, G916, G1510, G1988, G2771, G2859, G2884, G2993), can be used to manipulate light responses such as shade avoidance. As these genes also alter plant architecture, they may find use in the ornamental horticulture industry.
Development and morphology: seed development, ripening and germination rate. A number of the presently disclosed transcription factor genes (e.g., G961) have been shown to modify seed development and germination rate, including when the seeds are in conditions normally unfavorable for germination (e.g., cold, heat or salt stress, or in the presence of ABA), and may, along with functional equivalogs, thus be used to modify and improve germination rates under adverse conditions.
Growth rate and development: fast growth. A number of the presently disclosed transcription factor genes, including G807, G1476, and G2617, could be used to accelerate seedling growth, and thereby allow a crop to become established faster. This would minimize exposure to stress conditions at early stages of growth when the plants are most sensitive. Additionally, it can allow a crop to grow faster than competing weed species.
A number of these transcription factors have also been shown to increase growth rate of mature plants to a significant extent, including more rapid growth and development of reproductive organs. This provides utility for regions with short growing seasons. Accelerating plant growth would also improve early yield or increase biomass at an earlier stage, when such is desirable (for example, in producing vegetable crops or forestry products).
General development and morphology: slow growth rate. A number of the presently disclosed transcription factor genes, including G652, G1013, G1100, G1468, G1535, G1549, G1779, G1938, G2765, G2784, G2826, G2834, G2851, G3091, and G3095, have been shown to have significant effects on retarding plant growth rate and development. These observations have included, for example, delayed growth and development of reproductive organs. Slow growing plants may be highly desirable to ornamental horticulturists, both for providing house plants that display little change in their appearance over time, or outdoor plants for which wild-type or rapid growth is undesirable (e.g., ornamental palm trees). Slow growth may also provide for a prolonged fruiting period, thus extending the harvesting season, particularly in regions with long growing seasons. Slow growth could also provide a prolonged period in which pollen is available for improved self- or cross-fertilization, or cross-fertilization of cultivars that normally flower over non-overlapping time periods. The latter aspect may be particularly useful to plants comprising two or more distinct grafted cultivars (e.g., fruit trees) with normally non-overlapping flowering periods.
General development and morphology: senescence. Presently disclosed transcription factor genes may be used to alter senescence responses in plants. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting has significant value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield. In an experimental setting, tobacco plants engineered to inhibit leaf senescence had a longer photosynthetic lifespan, and produced a 50% increase in dry weight and seed yield (Gan and Amasino (1995) Science 270: 1986–1988). Delayed flower senescence caused by knocking out G652 or overexpressing G571, G2536, for example, may generate plants that retain their blossoms longer and this may be of potential interest to the ornamental horticulture industry, and delayed foliar and fruit senescence could improve post-harvest shelf-life of produce.
Premature senescence caused by, for example, G652, G1033, G1128, G1772, G2467, G2574, G2783, G2907, G3059, G3111 and their equivalogs may be used to improve a plant's response to disease and hasten fruit ripening.
Growth rate and development: lethality and necrosis. Overexpression of transcription factors, for example, G12, G366, G1384, G1556, G1840, G1832, G1840, G1850, G1957, G1990, G2213, G2298, G2505, G2570, G2587, G2869, G2887 and their equivalogs that have a role in regulating cell death may be used to induce lethality in specific tissues or necrosis in response to pathogen attack. For example, if a transcription factor gene inducing lethality or necrosis was specifically active in gametes (e.g., (G846), embryos (e.g., G374 knockouts) or reproductive organs, its expression in these tissues would lead to ablation and subsequent male or female sterility. Alternatively, under pathogen-regulated expression, a necrosis-inducing transcription factor can restrict the spread of a pathogen infection through a plant.
Plant Size: Large Plants and Increased Biomass.
Plants overexpressing G46, G268, G287, G314, G319, G324, G438, G624, G852, G1113, G1150, G1451, G1468, G2334, G2536, G2650, G2741, and G2979, for example, have been shown to be larger than controls. For some ornamental plants, the ability to provide larger varieties with these genes or their equivalogs may be highly desirable. More significantly, crop species overexpressing these genes from diverse species would also produce higher yields on larger cultivars, particularly those in which the vegetative portion of the plant is edible.
Overexpression of these genes can confer increased stress tolerance as well as increased biomass, and the increased biomass appears to be related to the particular mechanism of stress tolerance exhibited by these genes. The decision for a lateral organ to continue growth and expansion versus entering late development phases (growth cessation and senescence) is controlled genetically and hormonally, including regulation at an organ size checkpoint (e.g., Mizukami (1001) Curr Opinion Plant Biol 4: 533–39; Mizukami and Fisher (2000) Proc. Natl. Acad. Sci. 97: 942–47; Hu et al. Plant Cell 15:1591)). Organ size is controlled by the meristematic competence of organ cells, with increased meristematic competence leading to increased organ size (both leaves and stems). Plant hormones can impact plant organ size, with ethylene pathway overexpression leading to reduced organ size. There are also suggestions that auxin plays a determinative role in organ size. Stress responses can impact hormone levels in plant tissues, including ABA and ethylene levels. Thus, overexpression of G1073 appears to alter environmental (e.g., stress) inputs to the organ size checkpoint, thus enhancing organ size
Plant size: large seedlings. Presently disclosed transcription factor genes, that produce large seedlings can be used to produce crops that become established faster. Large seedlings are generally hardier, less vulnerable to stress, and better able to out-compete weed species. Seedlings in which expression of some of the presently disclosed transcription factors, including G1313, G2679, G2694, and G2838, for example, was modified, have been shown to possess larger cotyledons and/or were more developmentally advanced than control plants. Rapid seedling development made possible by manipulating expression of these genes or their equivalogs is likely to reduce loss due to diseases particularly prevalent at the seedling stage (e.g., damping off) and is thus important for survivability of plants germinating in the field or in controlled environments.
Plant size: dwarfed plants. Presently disclosed transcription factor genes, including G131, G136, G253, G309, G370, G386, G549, G550, G600, G651, G652, G707, G738, G811, G1011, G1100, G1247, G1289, G1340, G1423, G1474, G1483, G1549, G1554, G1593, G1753, G1772, G1779, G1798, G1938, G1983, G1993, G2085, G2226, G2227, G2251, G2372, G2375, G2453, G2456, G2459, G2492, G2515, G2550, G2565, G2574, G2575, G2579, G2616, G2628, G2640, G2649, G2682, G2702, G2757, G2783, G2839, G2846, G2847, G2850, G2884, G2934, G2958, G2979, G2992, G3017, G3059, G3091, and G3111 and their equivalogs can be used to decrease plant stature and may produce plants that are more resistant to damage by wind and rain, have improved lodging resistance, or more resistant to heat or low humidity or water deficit. Dwarf plants are also of significant interest to the ornamental horticulture industry, and particularly for home garden applications for which space availability may be limited.
Growth rate and development: Cell proliferation and differentiation. Transcription factors may be used regulate cell proliferation and/or differentiation in plants. Control of these processes could have valuable applications in plant transformation, cell culture or micro-propagation systems, as well as in control of the proliferation of particular useful tissues or cell types. Transcription factors that induce the proliferation of undifferentiated cells, such as G1539, G1585, G1591, G2885, and G2983, can be operably linked with an inducible promoter to promote the formation of callus that can be used for transformation or production of cell suspension cultures. Transcription factors that promote differentiation of shoots could be used in transformation or micro-propagation systems, where regeneration of shoots from callus is currently problematic. In addition, transcription factors that regulate the differentiation of specific tissues could be used to increase the proportion of these tissues in a plant. Transcription factors may promote the differentiation of carpel tissue, and these genes could be applied to commercial species to induce formation of increased numbers of carpels or fruits. A particular application might exist in saffron, one of the world's most expensive spices. Saffron filaments, or threads, are actually the dried stigmas of the saffron flower, Crocus sativus Linneaus . Each flower contains only three stigmas, and more than 75,000 of these flowers are needed to produce just one pound of saffron filaments. An increase in carpel number would increase the quantity of stigmatic tissue and improve yield.
Growth rate and development: cell expansion. Plant growth results from a combination of cell division and cell expansion. Transcription factors may be useful in regulation of cell expansion. Altered regulation of cell expansion (for example, by G521) could affect stem length, an important agronomic characteristic. For instance, short cultivars of wheat contributed to the Green Revolution, because plants that put fewer resources into stem elongation allocate more resources into developing seed and produce higher yield. These plants are also less vulnerable to wind and rain damage. These cultivars were found to be altered in their sensitivity to gibberellins, hormones that regulate stem elongation through control of both cell expansion and cell division. Altered cell expansion in leaves could also produce novel and ornamental plant forms.
Leaf morphology: dark leaves. Color-affecting components in leaves include chlorophylls (generally green), anthocyanins (generally red to blue) and carotenoids (generally yellow to red). Transcription factor genes that increase these pigments in leaves, including G30, G253, G309, G707, G811, G957, G1100, G1128, G1327, G1341, G1357, G1389, G1420, G1423, G1452, G1482, G1510, G1535, G1549, G1554, G1593, G1743, G1792, G1796, G1846, G1863, G1932, G1938, G1983, G2085, G2146, G2207, G2226, G2251, G2334, G2371, G2372, G2453, G2456, G2457, G2459, G2550, G2640, G2649, G2661, G2690, G2694, G2771, G2763, G2784, G2837, G2838, G2846, G2847, G2850, G2851, G2958, G2993, G3021, G3059, G3091, G3095, and G3111, may positively affect a plant's value to the ornamental horticulture industry. Variegated varieties, in particular, would show improved contrast. Other uses that result from overexpression of transcription factor genes include improvements in the nutritional value of foodstuffs. For example, lutein is an important nutraceutical; lutein-rich diets have been shown to help prevent age-related macular degeneration (ARMD), the leading cause of blindness in elderly people. Consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of ARMD.
Enhanced chlorophyll and carotenoid levels could also improve yield in crop plants. Lutein, like other xanthophylls such as zeaxanthin and violaxanthin, is an essential component in the protection of the plant against the damaging effects of excessive light. Specifically, lutein contributes, directly or indirectly, to the rapid rise of non-photochemical quenching in plants exposed to high light. Crop plants engineered to contain higher levels of lutein could therefore have improved photo-protection, leading to less oxidative damage and better growth under high light (e.g., during long summer days, or at higher altitudes or lower latitudes than those at which a non-transformed plant would thrive). Additionally, elevated chlorophyll levels increases photosynthetic capacity.
Leaf morphology: changes in leaf shape. Presently disclosed transcription factors produce marked and diverse effects on leaf development and shape, and include G30 and many others (see Table 6, “Change in leaf shape”). At early stages of growth, transgenic seedlings have developed narrow, upward pointing leaves with long petioles, possibly indicating a disruption in circadian-clock controlled processes or nyctinastic movements. Other transcription factor genes can be used to alter leaf shape in a significant manner from wild-type, some of which may find use in ornamental applications.
Leaf morphology: altered leaf size. Large leaves, such as those produced in plants overexpressing G268, G324, G438, G852, G1113, G1274, G1451, G2536, G2699, G2768, and G3008, generally increase plant biomass. This provides benefit for crops where the vegetative portion of the plant is the marketable portion.
Leaf morphology: light green and gray leaves. Transcription factor genes such as G351, G600, G651, G1468, G1718, G2565, G2604, G2779, G2859, G3044, and G3070 that provide an altered appearance may positively affect a plant's value to the ornamental horticulture industry.
Leaf morphology: glossy leaves. Transcription factor genes such as G30, G370 (when knocked-out), G975, G1792, G2640, G2649 and their equivalogs that induce the formation of glossy leaves generally do so by elevating levels of epidermal wax. Thus, the genes could be used to engineer changes in the composition and amount of leaf surface components, including waxes. The ability to manipulate wax composition, amount, or distribution could modify plant tolerance to drought and low humidity, or resistance to insects or pathogens. Additionally, wax may be a valuable commodity in some species, and altering its accumulation and/or composition could enhance yield.
Seed morphology: altered seed coloration. Presently disclosed transcription factor genes, including G581, G961, G2085, and G2371, have been used to modify seed color, which, along with the equivalogs of these genes, could provide added appeal to seeds or seed products.
Seed morphology: altered seed size and shape. The introduction of presently disclosed transcription factor genes, including G151, G581, G2085, G2585, or G2933, into plants that increase the size of seeds may have a significant impact on yield and appearance, particularly when the product is the seed itself (e.g., in the case of grains, legumes, nuts, etc.). Seed size, in addition to seed coat integrity, thickness and permeability, seed water content and a number of other components including antioxidants and oligosaccharides, also affects affect seed longevity in storage, with larger seeds often being more desirable for prolonged storage.
Transcription factor genes that alter seed shape, including G652, G916, G961 and their equivalogs may have both ornamental applications and improve or broaden the appeal of seed products.
Leaf and seed biochemistry. Overexpression of transcription factors genes, including G975 and its equivalogs, which results in increased leaf wax could be used to manipulate wax composition, amount, or distribution. These transcription factors can improve yield in those plants and crops from which wax is a valuable product. The genes may also be used to modify plant tolerance to drought and/or low humidity or resistance to insects, as well as plant appearance (glossy leaves). The effect of increased wax deposition on leaves of a plant like may improve water use efficiency. Manipulation of these genes may reduce the wax coating on sunflower seeds; this wax fouls the oil extraction system during sunflower seed processing for oil. For the latter purpose or any other where wax reduction is valuable, antisense or co-suppression of the transcription factor genes in a tissue-specific manner would be valuable.
Prenyl lipids play a role in anchoring proteins in membranes or membranous organelles. Thus modifying the prenyl lipid content of seeds and leaves could affect membrane integrity and function. One important group of prenyl lipids, the tocopherols, have both anti-oxidant and vitamin E activity. Transcription factor genes (e.g., a G652 knockout) have been shown to modify the prenyl lipid content of leaves in plants, and these genes and their equivalogs may thus be used to alter prenyl lipid content of leaves.
Overexpression of transcription factors have resulted in plants with altered leaf insoluble sugar content. These transcription factors and their equivalogs that alter plant cell wall composition have several potential applications including altering food digestibility, plant tensile strength, wood quality, pathogen resistance and in pulp production. In particular, hemicellulose is not desirable in paper pulps because of its lack of strength compared with cellulose. Thus modulating the amounts of cellulose vs. hemicellulose in the plant cell wall is desirable for the paper/lumber industry. Increasing the insoluble carbohydrate content in various fruits, vegetables, and other edible consumer products will result in enhanced fiber content. Increased fiber content would not only provide health benefits in food products, but might also increase digestibility of forage crops. In addition, the hemicellulose and pectin content of fruits and berries affects the quality of jam and catsup made from them. Changes in hemicellulose and pectin content could result in a superior consumer product.
A number of the presently disclosed transcription factor genes have been shown to alter the fatty acid composition in plants (e.g., G975), and seeds and leaves in particular. This modification suggests several utilities, including improving the nutritional value of seeds or whole plants. Dietary fatty acids ratios have been shown to have an effect on, for example, bone integrity and remodeling (see, for example, Weiler (2000) Pediatr. Res. 47:5 692–697). The ratio of dietary fatty acids may alter the precursor pools of long-chain polyunsaturated fatty acids that serve as precursors for prostaglandin synthesis. In mammalian connective tissue, prostaglandins serve as important signals regulating the balance between resorption and formation in bone and cartilage. Thus dietary fatty acid ratios altered in seeds may affect the etiology and outcome of bone loss.
Transcription factors that reduce leaf fatty acids, for example, 16:3 fatty acids, may be used to control thylakoid membrane development, including proplastid to chloroplast development. The genes that encode these transcription factors might thus be useful for controlling the transition from proplastid to chromoplast in fruits and vegetables. It may also be desirable to change the expression of these genes to prevent cotyledon greening in Brassica napus or B. campestris to avoid green oil due to early frost.
Transcription factor genes that increase leaf fatty acid production, including G975 and its equivalogs could potentially be used to manipulate seed composition, which is very important for the nutritional value and production of various food products. A number of transcription factor genes are involved in mediating an aspect of the regulatory response to temperature. These genes may be used to alter the expression of desaturases that lead to production of 18:3 and 16:3 fatty acids, the balance of which affects membrane fluidity and mitigates damage to cell membranes and photosynthetic structures at high and low temperatures.
The G652 knockout line had a reproducible increase in the leaf glucosinolate M39480. It also showed a reproducible increase in seed alpha-tocopherol. A number of glucosinolates have been shown to have anti-cancer activity; thus, increasing the levels or composition of these compounds by modifying the expression of transcription factors (e.g., G652), can have a beneficial effect on human diet.
Glucosinolates are undesirable components of the oilseeds used in animal feed since they produce toxic effects. Low-glucosinolate varieties of canola, for example, have been developed to combat this problem. Glucosinolates form part of a plant's natural defense against insects. Modification of glucosinolate composition or quantity by introducing transcription factors that affect these characteristics can therefore afford increased protection from herbivores. Furthermore, in edible crops, tissue specific promoters can be used to ensure that these compounds accumulate specifically in tissues, such as the epidermis, which are not taken for consumption.
Presently disclosed transcription factor genes that modify levels of phytosterols in plants may have at least two utilities. First, phytosterols are an important source of precursors for the manufacture of human steroid hormones. Thus, regulation of transcription factor expression or activity could lead to elevated levels of important human steroid precursors for steroid semi-synthesis. For example, transcription factors that cause elevated levels of campesterol in leaves, or sitosterols and stigmasterols in seed crops, would be useful for this purpose. Phytosterols and their hydrogenated derivatives phytostanols also have proven cholesterol-lowering properties, and transcription factor genes that modify the expression of these compounds in plants would thus provide health benefits.
The composition of seeds, particularly with respect to seed oil amounts and/or composition, is very important for the nutritional and caloric value and production of various food and feed products. Modifying the expression of transcription factor genes that alter seed oil content could be used to improve the heat stability of oils or to improve the nutritional quality of seed oil, by, for example, reducing the number of calories in seed by decreasing oil or fatty acid content, OR increasing the number of calories in animal feeds by increasing fatty acid or seed oil content (e.g., by knocking out G961, G1451, or G2830).
As with seed oils, the composition of seeds, particularly with respect to protein amounts and/or composition, is very important for the nutritional value and production of various food and feed products. Transcription factor genes may be used to modify protein concentrations in seeds, which would modify the caloric content of seeds or provide nutritional benefits, and may be used to prolong storage, increase seed pest or disease resistance, or modify germination rates.
Prenyl lipids play a role in anchoring proteins in membranes or membranous organelles. Thus, presently disclosed transcription factor genes, including G652 and equivalogs, that modify the prenyl lipid content of seeds and leaves (in the case of G652, when this gene is knocked out) could affect membrane integrity and function. Transcription factor genes have been shown to modify the tocopherol composition of plants. α-Tocopherol is better known as vitamin E. Tocopherols such as α- and γ-tocopherol both have anti-oxidant activity.
Light response/shade avoidance: altered cotyledon, hypocotyl, petiole development, altered leaf orientation, constitutive photomorphogenesis, photomorphogenesis in low light. Presently disclosed transcription factor genes, including G30; G246; G351, G353; G354; G478, G807, G916, G1013, G1082, G1510, G1988, G2432; G2650; G2694, G2754, G2771, G2859, G2884, G2993, G3032 and their equivalogs that can modify a plant's response to light may be useful for modifying plant growth or development, for example, photomorphogenesis in poor light, or accelerating flowering time in response to various light intensities, quality or duration to which a non-transformed plant would not similarly respond. Examples of such responses that have been demonstrated include leaf number and arrangement, and early flower bud appearances. Elimination of shading responses may lead to increased planting densities with subsequent yield enhancement. As these genes may also alter plant architecture, they may find use in the ornamental horticulture industry.
Pigment: Increased Anthocyanin Level in Various Plant Organs and Tissues.
G253, G386, G581, G707, G1482, G2453, G2456, G2459, G2604, G2718 and equivalogs can be used to alter anthocyanin levels in one or more tissues, depending on the organ in which these genes are expressed may be used to alter anthocyanin production in numerous plant species. Expression of presently disclosed transcription factor genes that increase flavonoid production in plants, including anthocyanins and condensed tannins, may be used to alter in pigment production for horticultural purposes, and possibly increasing stress resistance. A number of flavonoids have been shown to have antimicrobial activity and could be used to engineer pathogen resistance. Several flavonoid compounds have health promoting effects such as inhibition of tumor growth, prevention of bone loss and prevention of the oxidation of lipids. Increased levels of condensed tannins, in forage legumes would be an important agronomic trait because they prevent pasture bloat by collapsing protein foams within the rumen. For a review on the utilities of flavonoids and their derivatives, refer to Dixon et al. (1999) Trends Plant Sci. 4: 394–400.
Antisense and Co-Suppression
In addition to expression of the nucleic acids of the invention as gene replacement or plant phenotype modification nucleic acids, the nucleic acids are also useful for sense and anti-sense suppression of expression, e.g. to down-regulate expression of a nucleic acid of the invention, e.g. as a further mechanism for modulating plant phenotype. That is, the nucleic acids of the invention, or subsequences or anti-sense sequences thereof, can be used to block expression of naturally occurring homologous nucleic acids. A variety of sense and anti-sense technologies are known in the art, e.g. as set forth in Lichtenstein and Nellen (1997) Antisense Technology: A Practical Approach IRL Press at Oxford University Press, Oxford, U.K. Antisense regulation is also described in Crowley et al. (1985) Cell 43: 633–641; Rosenberg et al. (1985) Nature 313: 703–706; Preiss et al. (1985) Nature 313: 27–32; Melton (1985) Proc. Natl. Acad. Sci. 82: 144–148; Izant and Weintraub (1985) Science 229: 345–352; and Kim and Wold (1985) Cell 42: 129–138. Additional methods for antisense regulation are known in the art. Antisense regulation has been used to reduce or inhibit expression of plant genes in, for example in European Patent Publication No. 271988. Antisense RNA may be used to reduce gene expression to produce a visible or biochemical phenotypic change in a plant (Smith et al. (1988) Nature, 334: 724–726; Smith et al. (1990) Plant Mol. Biol. 14: 369–379). In general, sense or anti-sense sequences are introduced into a cell, where they are optionally amplified, e.g. by transcription. Such sequences include both simple oligonucleotide sequences and catalytic sequences such as ribozymes.
For example, a reduction or elimination of expression (i.e., a “knock-out”) of a transcription factor or transcription factor homolog polypeptide in a transgenic plant, e.g., to modify a plant trait, can be obtained by introducing an antisense construct corresponding to the polypeptide of interest as a cDNA. For antisense suppression, the transcription factor or homolog cDNA is arranged in reverse orientation (with respect to the coding sequence) relative to the promoter sequence in the expression vector. The introduced sequence need not be the full length cDNA or gene, and need not be identical to the cDNA or gene found in the plant type to be transformed. Typically, the antisense sequence need only be capable of hybridizing to the target gene or RNA of interest. Thus, where the introduced sequence is of shorter length, a higher degree of homology to the endogenous transcription factor sequence will be needed for effective antisense suppression. While antisense sequences of various lengths can be utilized, preferably, the introduced antisense sequence in the vector will be at least 30 nucleotides in length, and improved antisense suppression will typically be observed as the length of the antisense sequence increases. Preferably, the length of the antisense sequence in the vector will be greater than 100 nucleotides. Transcription of an antisense construct as described results in the production of RNA molecules that are the reverse complement of mRNA molecules transcribed from the endogenous transcription factor gene in the plant cell.
Suppression of endogenous transcription factor gene expression can also be achieved using RNA interference, or RNAi. RNAi is a post-transcriptional, targeted gene-silencing technique that uses double-stranded RNA (dsRNA) to incite degradation of messenger RNA (mRNA) containing the same sequence as the dsRNA (Constans, (2002) The Scientist 16:36). Small interfering RNAs, or siRNAs are produced in at least two steps: an endogenous ribonuclease cleaves longer dsRNA into shorter, 21–23 nucleotide-long RNAs. The siRNA segments then mediate the degradation of the target mRNA (Zamore, (2001) Nature Struct. Biol., 8:746–50). RNAi has been used for gene function determination in a manner similar to antisense oligonucleotides (Constans, (2002) The Scientist 16:36). Expression vectors that continually express siRNAs in transiently and stably transfected cells have been engineered to express small hairpin RNAs (shRNAs), which get processed in vivo into siRNAs-like molecules capable of carrying out gene-specific silencing (Brummelkamp et al., (2002) Science 296:550–553, and Paddison, et al. (2002) Genes & Dev. 16:948–958). Post-transcriptional gene silencing by double-stranded RNA is discussed in further detail by Hammond et al. (2001) Nature Rev Gen 2: 110–119, Fire et al. (1998) Nature 391: 806–811 and Timmons and Fire (1998) Nature 395: 854. Vectors in which RNA encoded by a transcription factor or transcription factor homolog cDNA is over-expressed can also be used to obtain co-suppression of a corresponding endogenous gene, e.g., in the manner described in U.S. Pat. No. 5,231,020 to Jorgensen. Such co-suppression (also termed sense suppression) does not require that the entire transcription factor cDNA be introduced into the plant cells, nor does it require that the introduced sequence be exactly identical to the endogenous transcription factor gene of interest. However, as with antisense suppression, the suppressive efficiency will be enhanced as specificity of hybridization is increased, e.g., as the introduced sequence is lengthened, and/or as the sequence similarity between the introduced sequence and the endogenous transcription factor gene is increased.
Vectors expressing an untranslatable form of the transcription factor mRNA, e.g., sequences comprising one or more stop codon, or nonsense mutation) can also be used to suppress expression of an endogenous transcription factor, thereby reducing or eliminating its activity and modifying one or more traits. Methods for producing such constructs are described in U.S. Pat. No. 5,583,021. Preferably, such constructs are made by introducing a premature stop codon into the transcription factor gene. Alternatively, a plant trait can be modified by gene silencing using double-stranded RNA (Sharp (1999) Genes and Development 13: 139–141). Another method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens . After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in a transcription factor or transcription factor homolog gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation. Such methods are well known to those of skill in the art (See for example Koncz et al. (1992) Methods in Arabidopsis Research , World Scientific Publishing Co. Pte. Ltd., River Edge, N.J.).
Alternatively, a plant phenotype can be altered by eliminating an endogenous gene, such as a transcription factor or transcription factor homolog, e.g., by homologous recombination (Kempin et al. (1997) Nature 389: 802–803).
A plant trait can also be modified by using the Cre-lox system (for example, as described in U.S. Pat. No. 5,658,772). A plant genome can be modified to include first and second lox sites that are then contacted with a Cre recombinase. If the lox sites are in the same orientation, the intervening DNA sequence between the two sites is excised. If the lox sites are in the opposite orientation, the intervening sequence is inverted.
The polynucleotides and polypeptides of this invention can also be expressed in a plant in the absence of an expression cassette by manipulating the activity or expression level of the endogenous gene by other means, such as, for example, by ectopically expressing a gene by T-DNA activation tagging (Ichikawa et al. (1997) Nature 390 698–701; Kakimoto et al. (1996) Science 274: 982–985). This method entails transforming a plant with a gene tag containing multiple transcriptional enhancers and once the tag has inserted into the genome, expression of a flanking gene coding sequence becomes deregulated. In another example, the transcriptional machinery in a plant can be modified so as to increase transcription levels of a polynucleotide of the invention (See, e.g., PCT Publications WO 96/06166 and WO 98/53057 which describe the modification of the DNA-binding specificity of zinc finger proteins by changing particular amino acids in the DNA-binding motif).
The transgenic plant can also include the machinery necessary for expressing or altering the activity of a polypeptide encoded by an endogenous gene, for example, by altering the phosphorylation state of the polypeptide to maintain it in an activated state.
Transgenic plants (or plant cells, or plant explants, or plant tissues) incorporating the polynucleotides of the invention and/or expressing the polypeptides of the invention can be produced by a variety of well established techniques as described above. Following construction of a vector, most typically an expression cassette, including a polynucleotide, e.g., encoding a transcription factor or transcription factor homolog, of the invention, standard techniques can be used to introduce the polynucleotide into a plant, a plant cell, a plant explant or a plant tissue of interest. Optionally, the plant cell, explant or tissue can be regenerated to produce a transgenic plant.
The plant can be any higher plant, including gymnosperms, monocotyledonous and dicotyledonous plants. Suitable protocols are available for Leguminosae (alfalfa, soybean, clover, etc.), Umbelliferae (carrot, celery, parsnip), Cruciferae (cabbage, radish, rapeseed, broccoli, etc.), Curcurbitaceae (melons and cucumber), Gramineae (wheat, corn, rice, barley, millet, etc.), Solanaceae (potato, tomato, tobacco, peppers, etc.), and various other crops. See protocols described in Ammirato et al., eds., (1984) Handbook of Plant Cell Culture—Crop Species , Macmillan Publ. Co., New York, N.Y.; Shimamoto et al. (1989) Nature 338: 274–276; Fromm et al. (1990) Bio/Technol. 8: 833–839; and Vasil et al. (1990) Bio/Technol. 8: 429–434.
Transformation and regeneration of both monocotyledonous and dicotyledonous plant cells is now routine, and the selection of the most appropriate transformation technique will be determined by the practitioner. The choice of method will vary with the type of plant to be transformed; those skilled in the art will recognize the suitability of particular methods for given plant types. Suitable methods can include, but are not limited to: electroporation of plant protoplasts; liposome-mediated transformation; polyethylene glycol (PEG) mediated transformation; transformation using viruses; micro-injection of plant cells; micro-projectile bombardment of plant cells; vacuum infiltration; and Agrobacterium tumefaciens mediated transformation. Transformation means introducing a nucleotide sequence into a plant in a manner to cause stable or transient expression of the sequence.
Successful examples of the modification of plant characteristics by transformation with cloned sequences which serve to illustrate the current knowledge in this field of technology, and which are herein incorporated by reference, include: U.S. Pat. Nos. 5,571,706; 5,677,175; 5,510,471; 5,750,386; 5,597,945; 5,589,615; 5,750,871; 5,268,526; 5,780,708; 5,538,880; 5,773,269; 5,736,369 and 5,610,042.
Following transformation, plants are preferably selected using a dominant selectable marker incorporated into the transformation vector. Typically, such a marker will confer antibiotic or herbicide resistance on the transformed plants, and selection of transformants can be accomplished by exposing the plants to appropriate concentrations of the antibiotic or herbicide.
After transformed plants are selected and grown to maturity, those plants showing a modified trait are identified. The modified trait can be any of those traits described above. Additionally, to confirm that the modified trait is due to changes in expression levels or activity of the polypeptide or polynucleotide of the invention can be determined by analyzing mRNA expression using Northern blots, RT-PCR or microarrays, or protein expression using immunoblots or Western blots or gel shift assays.
Integrated Systems—Sequence Identity
Additionally, the present invention may be an integrated system, computer or computer readable medium that comprises an instruction set for determining the identity of one or more sequences in a database. In addition, the instruction set can be used to generate or identify sequences that meet any specified criteria. Furthermore, the instruction set may be used to associate or link certain functional benefits, such improved characteristics, with one or more identified sequence.
For example, the instruction set can include, e.g., a sequence comparison or other alignment program, e.g., an available program such as, for example, the Wisconsin Package Version 10.0, such as BLAST, FASTA, PILEUP, FINDPATTERNS or the like (GCG, Madison, Wis.). Public sequence databases such as GenBank, EMBL, Swiss-Prot and PIR or private sequence databases such as PHYTOSEQ sequence database (Incyte Genomics, Palo Alto, Calif.) can be searched.
Alignment of sequences for comparison can be conducted by the local homology algorithm of Smith and Waterman (1981) Adv. Appl. Math. 2: 482–489, by the homology alignment algorithm of Needleman and Wunsch (1970) J. Mol. Biol. 48: 443–453, by the search for similarity method of Pearson and Lipman (1988) Proc. Natl. Acad. Sci. 85: 2444–2448, by computerized implementations of these algorithms. After alignment, sequence comparisons between two (or more) polynucleotides or polypeptides are typically performed by comparing sequences of the two sequences over a comparison window to identify and compare local regions of sequence similarity. The comparison window can be a segment of at least about 20 contiguous positions, usually about 50 to about 200, more usually about 100 to about 150 contiguous positions. A description of the method is provided in Ausubel et al. supra.
A variety of methods for determining sequence relationships can be used, including manual alignment and computer assisted sequence alignment and analysis. This later approach is a preferred approach in the present invention, due to the increased throughput afforded by computer assisted methods. As noted above, a variety of computer programs for performing sequence alignment are available, or can be produced by one of skill.
One example algorithm that is suitable for determining percent sequence identity and sequence similarity is the BLAST algorithm, which is described in Altschul et al. (1990) J. Mol. Biol. 215: 403–410. Software for performing BLAST analyses is publicly available, e.g., through the National Library of Medicine's National Center for Biotechnology Information (ncbi.nlm.nih; see at world wide web (www) National Institutes of Health US government (gov) website). This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is referred to as the neighborhood word score threshold (Altschul et al. supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always >0) and N (penalty score for mismatching residues; always <0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. The BLASTN program (for nucleotide sequences) uses as defaults a wordlength (W) of 11, an expectation (E) of 10, a cutoff of 100, M=5, N=−4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as defaults a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see Henikoff and Henikoff (1992) Proc. Natl. Acad. Sci. 89: 10915–10919). Unless otherwise indicated, “sequence identity” here refers to the % sequence identity generated from a tblastx using the NCBI version of the algorithm at the default settings using gapped alignments with the filter “off” (see, for example, NIH NLM NCBI website at ncbi.nlm.nih, supra).
In addition to calculating percent sequence identity, the BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g. Karlin and Altschul (1993) Proc. Natl. Acad. Sci. 90: 5873–5787). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability by which a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence (and, therefore, in this context, homologous) if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.1, or less than about 0.01, and or even less than about 0.001. An additional example of a useful sequence alignment algorithm is PILEUP. PILEUP creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments. The program can align, e.g., up to 300 sequences of a maximum length of 5,000 letters.
The integrated system, or computer typically includes a user input interface allowing a user to selectively view one or more sequence records corresponding to the one or more character strings, as well as an instruction set which aligns the one or more character strings with each other or with an additional character string to identify one or more region of sequence similarity. The system may include a link of one or more character strings with a particular phenotype or gene function. Typically, the system includes a user readable output element that displays an alignment produced by the alignment instruction set.
The methods of this invention can be implemented in a localized or distributed computing environment. In a distributed environment, the methods may be implemented on a single computer comprising multiple processors or on a multiplicity of computers. The computers can be linked, e.g. through a common bus, but more preferably the computer(s) are nodes on a network. The network can be a generalized or a dedicated local or wide-area network and, in certain preferred embodiments, the computers may be components of an intra-net or an internet.
Thus, the invention provides methods for identifying a sequence similar or homologous to one or more polynucleotides as noted herein, or one or more target polypeptides encoded by the polynucleotides, or otherwise noted herein and may include linking or associating a given plant phenotype or gene function with a sequence. In the methods, a sequence database is provided (locally or across an inter or intra net) and a query is made against the sequence database using the relevant sequences herein and associated plant phenotypes or gene functions.
Any sequence herein can be entered into the database, before or after querying the database. This provides for both expansion of the database and, if done before the querying step, for insertion of control sequences into the database. The control sequences can be detected by the query to ensure the general integrity of both the database and the query. As noted, the query can be performed using a web browser based interface. For example, the database can be a centralized public database such as those noted herein, and the querying can be done from a remote terminal or computer across an internet or intranet.
Any sequence herein can be used to identify a similar, homologous, paralogous, or orthologous sequence in another plant. This provides means for identifying endogenous sequences in other plants that may be useful to alter a trait of progeny plants, which results from crossing two plants of different strain. For example, sequences that encode an ortholog of any of the sequences herein that naturally occur in a plant with a desired trait can be identified using the sequences disclosed herein. The plant is then crossed with a second plant of the same species but which does not have the desired trait to produce progeny which can then be used in further crossing experiments to produce the desired trait in the second plant. Therefore the resulting progeny plant contains no transgenes; expression of the endogenous sequence may also be regulated by treatment with a particular chemical or other means, such as EMR. Some examples of such compounds well known in the art include: ethylene; cytokinins; phenolic compounds, which stimulate the transcription of the genes needed for infection; specific monosaccharides and acidic environments which potentiate vir gene induction; acidic polysaccharides which induce one or more chromosomal genes; and opines; other mechanisms include light or dark treatment (for a review of examples of such treatments, see, Winans (1992) Microbiol. Rev. 56: 12–31; Eyal et al. (1992) Plant Mol. Biol. 19: 589–599; Chrispeels et al. (2000) Plant Mol. Biol. 42: 279–290; Piazza et al. (2002) Plant Physiol. 128: 1077–1086).
Table 7 lists sequences discovered to be orthologous to a number of representative transcription factors of the present invention. The column headings include the transcription factors listed by (a) the SEQ ID NO: of the Arabidopsis sequence that was used to discover the non- Arabidopsis orthologous sequence; (b) the GID sequence identifier of the Arabidopsis sequence; (c) the Sequence Identifier or GenBank Accession Number of the orthologous sequence; (d) the species from which the orthologous sequence is derived; (e) the SEQ ID NO: of the non- Arabidopsis orthologous sequence, and (e) the smallest sum probability pairwise comparison of each orthologous sequence to the similar Arabidopsis sequence determined by BLAST analysis.





TABLE 7



Orthologs of Representative Arabidopsis Transcription Factor Genes








SEQ ID NO:






of


Arabidopsis





Smallest Sum

Sequence




Probability to

Used to




Ortholog,

Discover
GID
Species from Which
Sequence Identifier or
SEQ ID NO: of
When

Ortholog
No.
Ortholog is Derived
Accession Number
Orthologous Sequence
Known










2
G2

Petunia x hybrida

AF132001

1.00E−122

2
G2

Pisum sativum

AF325506

1.00E−121

2
G2

Antirrhinum majus

AY223518

1.00E−120

2
G2

Malus x domestica

AF332215

1.00E−119

2
G2

Lycopersicon

BM412075

1.00E−105




esculentum


2
G2

Picea abies

AF253970

1.00E−103

2
G2

Solanum tuberosum

BQ120583
3.00E−98

2
G2

Oryza sativa

CB649669

1.00E−96



( japonica cultivar-



group)

2
G2

Lactuca sativa

BU000526

1.00E−93

2
G2
Oryza sativa ( indica
CB624207

5.00E−92



cultivar-group)

2
G2

Petunia x hybrida

gi5081555

2.70E−123

2
G2

Pisum sativum

gi13173164

1.50E−117

2
G2

Antirrhinum majus

gi28894443

8.20E−113

2
G2

Malus x domestica

gi21717332

2.00E−111

2
G2

Oryza sativa

gi32483001

6.90E−105



( japonica cultivar-



group)

2
G2

Picea abies

gi11181610

8.20E−102

2
G2

Hordeum vulgare

gi18476518

3.00E−85

2
G2

Zea mays

gi2944040

1.00E−84

2
G2

Hyacinthus

gi5360996

1.90E−79




orientalis


2
G2

Glycine max

gi25898745

2.60E−46

3
G12

Glycine max

BG045111.1
671

3
G12

Glycine max

GLYMA-28NOV01-
672




CLUSTER22720_1

3
G12

Glycine max

GLYMA-28NOV01-
673




CLUSTER22720_2

3
G12

Glycine max

GLYMA-28NOV01-
674




CLUSTER272_47

3
G12

Glycine max

GLYMA-28NOV01-
675




CLUSTER272_51

3
G12

Glycine max

GLYMA-28NOV01-
676




CLUSTER59385_1

3
G12

Glycine max

LIB3093-031-Q1-K1-
677




C10

3
G12

Oryza sativa

ORYSA-22JAN02-
678




CLUSTER160586_1

3
G12

Oryza sativa

ORYSA-22JAN02-
679




CLUSTER76674_1

3
G12

Oryza sativa

ORYSA-22JAN02-
680




CLUSTER76674_4

3
G12

Oryza sativa

OSC102287.C1.p11.fg
681

3
G12

Oryza sativa

OSC15346.C1.p71.fg
682

3
G12

Oryza sativa

OSC32395.C1.p3.fg
683

3
G12

Zea mays

ZEAMA-O8NOV01-
684




CLUSTER88196_1

3
G12

Oryza sativa

Os_S32369
1557

3
G12

Oryza sativa

Os_S80194
1558

3
G12

Glycine max

Gma_S5071803
1632

3
G12

Medicago

Mtr_S5349908
1690




truncatula


3
G12

Lycopersicon

SGN-UNIGENE-49683
1937




esculentum


3
G12

Lycopersicon

SGN-UNIGENE-54594
1938




esculentum


3
G12

Lycopersicon

SGN-UNIGENE-
1939




esculentum

SINGLET-47313

4
G12

Brassica oleracea

BH429963

4.00E−85

4
G12

Gossypium

BQ405872

3.00E−51




arboreum


4
G12

Medicago

BG648561

2.00E−47




truncatula


4
G12

Thellungiella

BM985484

5.00E−47




halophila


4
G12

Brassica napus

CD834636

6.00E−46

4
G12

Glycine max

BG046836

5.00E−40

4
G12

Lupinus albus

CA526351

1.00E−39

4
G12

Oryza sativa

OSJN00057

1.00E−39

4
G12

Oryza sativa

AK061095

1.00E−39



( japonica cultivar-



group)

4
G12

Helianthus

CF089073

2.00E−39




argophyllus


4
G12

Oryza sativa

gi21740879

8.40E−40



( japonica cultivar-



group)

4
G12

Oryza sativa

gi5091503

1.30E−38

4
G12

Glycine max

gi31324058

2.60E−36

4
G12

Lycopersicon

gi27436378

7.30E−22




esculentum


4
G12

Zea mays

gi21908034

7.30E−22

4
G12

Catharanthus

gi8980313

3.40E−20




roseus


4
G12

Stylosanthes hamata

gi4099921

4.80E−19

4
G12

Fagus sylvatica

gi18496063

2.70E−18

4
G12

Hordeum vulgare

gi27960757

8.40E−18

4
G12

Nicotiana tabacum

gi10798644

9.30E−18

6
G15

Brassica napus

BD274518

1.0e−999

6
G15

Glycine max

AX555216

1.00E−131

6
G15

Oryza sativa

AK106306

1.00E−116



( japonica cultivar-



group)

6
G15

Oryza sativa

AX555220

1.00E−116

6
G15

Nuphar advena

CD475882

1.00E−91

6
G15

Zea mays

AY109146

5.00E−89

6
G15

Brassica oleracea

BZ056530

1.00E−88

6
G15

Physcomitrella

BJ188928

3.00E−84



patens subsp. patens

6
G15

Lactuca sativa

BQ864461

1.00E−76

6
G15

Triticum aestivum

BJ312281

6.00E−73

6
G15

Glycine max

gi25898745

1.50E−132

6
G15

Oryza sativa

gi28201307

1.70E−122



( japonica cultivar-



group)

6
G15

Oryza sativa

gi25898752

6.90E−118

6
G15

Brassica napus

gi21069051

1.00E−88

6
G15

Zea mays

gi2652938

7.00E−84

6
G15

Malus x domestica

gi21717332

1.50E−45

6
G15

Picea abies

gi11181612

1.70E−45

6
G15

Pisum sativum

gi13173164

1.90E−45

6
G15

Hordeum vulgare

gi18476518

3.40E−43

6
G15

Antirrhinum majus

gi28894443

9.10E−43

7
G30

Oryza sativa

G3381
2126
5.00E−33

7
G30

Glycine max

AW308784.1
685

7
G30

Glycine max

BG790680.1
686

7
G30

Glycine max

GLYMA-28NOV01-
687




CLUSTER602185_1

7
G30

Glycine max

GLYMA-28NOV01-
688




CLUSTER91218_1

7
G30

Glycine max

LIB5118-009-Q1-PF1-F2
689

7
G30

Oryza sativa

OSC20174.C1.p2.fg
690

7
G30

Zea mays

LIB4756-134-A1-K1-
691




G10

7
G30

Oryza sativa

Os_S102414
1559

7
G30

Glycine max

Gma_S5001644
1633

7
G30

Zea mays

Zm_S11513768
1754

7
G30

Triticum aestivum

Ta_S274849
1834

8
G30

Brassica oleracea

BH517030

1.00E−37

8
G30

Lycopersicon

AI776626

2.00E−35




esculentum


8
G30

Triticum aestivum

BT009060

2.00E−33

8
G30

Sorghum bicolor

BZ337899

1.00E−32

8
G30

Eucalyptus grandis

CB967722

1.00E−31

8
G30

Zea mays

CC349655

1.00E−31

8
G30

Oryza sativa

AP004623

3.00E−31



( japonica cultivar-



group)

8
G30
Oryza sativa ( indica
AAAA01005323

3.00E−31



cultivar-group)

8
G30

Oryza sativa

AP003891

3.00E−31

8
G30

Glycine max

BG790680

4.00E−29

8
G30

Oryza sativa

gi28071302

3.60E−32



( japonica cultivar-



group)

8
G30

Lycopersicon

gi2213783

7.90E−26




esculentum


8
G30

Catharanthus

gi8980313

4.70E−24




roseus


8
G30

Matricaria

gi17385636

1.10E−23




chamomilla


8
G30

Oryza sativa

gi12597874

1.80E−23

8
G30

Mesembryanthemum

gi32401273

3.70E−23




crystallinum


8
G30

Nicotiana tabacum

gi1732406

5.20E−23

8
G30

Nicotiana sylvestris

gi8809571

8.70E−22

8
G30

Cicer arietinum

gi24817250

1.10E−21

8
G30

Glycine max

gi21304712

1.40E−21

9
G46

Glycine max

GLYMA-28NOV01-
692




CLUSTER15812_1

9
G46

Glycine max

OLYMA-28NOV01-
693




CLUSTER15812_2

9
G46

Glycine max

OLYMA-28NOV01-
694




CLUSTER164789_1

9
G46

Glycine max

OLYMA-28NOV01-
695




CLUSTER164789_2

9
G46

Glycine max

GLYMA-28NOV01-
696




CLUSTER2315_2

9
G46

Glycine max

OLYMA-28NOV01-
697




CLUSTER605_217

9
G46

Glycine max

OLYMA-28NOV01-
698




CLUSTER605_219

9
G46

Glycine max

OLYMA-28NOV01-
699




CLUSTER91242_1

9
G46

Oryza sativa

OSC101836.C1.p4.fg
700

9
G46

Zea mays

LIB3062-015-Q1-K1-F11
701

9
G46

Lycopersicon

Les_S5295471
1925




esculentum


9
G46

Lycopersicon

SGN-UNIGENE-44432
1940




esculentum


9
G46

Lycopersicon

SGN-UNIGENE-49046
1941




esculentum


9
G46

Lycopersicon

SGN-UNIGENE-49310
1942




esculentum


10
G46

Brassica oleracea

BH970151

3.00E−83

10
G46

Brassica napus

CD834612

3.00E−70

10
G46

Lycopersicon

AY192370

3.00E−38




esculentum


10
G46

Solanum tuberosum

BG592132

2.00E−37

10
G46

Medicago

BF637755

4.00E−32




truncatula


10
G46

Glycine max

BQ740307

7.00E−31

10
G46

Lotus japonicus

AV423260

4.00E−30

10
G46

Populus tremula x

BU814218

8.00E−30




Populus tremuloides


10
G46

Populus

BU871861

8.00E−30



balsamifera subsp.




trichocarpa


10
G46

Vitis vinifera

BM436925

2.00E−29

10
G46

Brassica oleracea

gi15054374

2.50E−47

10
G46

Lycopersicon

gi28274834

4.20E−38




esculentum


10
G46

Nicotiana tabacum

gi1208497

5.90E−31

10
G46

Nicotiana sylvestris

gi8809575

3.50E−29

10
G46

Oryza sativa

gi14140141

4.50E−29

10
G46

Matricaria

gi17385636

1.80E−28




chamomilla


10
G46

Mesembryanthemum

gi32401273

5.00E−28




crystallinum


10
G46

Oryza sativa

gi31433532

1.00E−27



( japonica cultivar-



group)

10
G46

Catharanthus

gi8980313

1.20E−24




roseus


10
G46

Glycine max

gi21304712

6.80E−22

11
G47

Glycine max

G3643
2225
2.00E−29

11
G47

Oryza sativa

G3644
2227
3.00E−25

11
G47

Brassica rapa

G3645
2229
1.00E−63

11
G47

Brassica oleracea

G3646
2231
2.00E−46

11
G47

Zinnia elegans

G3647
2233
3.00E−33

11
G47

Oryza sativa

G3649
2235
4.00E−23

11
G47

Oryza sativa

G3651
2237
3.00E−20

11
G47

Glycine max

GLYMA-28NOV01-
702




CLUSTER115749_1

11
G47

Oryza sativa

OSC21268.C1.p12.fg
703

11
G47

Hordeum vulgare

Hv_S7318
1718

12
G47

Brassica rapa

BG543936

2.00E−60


subsp. pekinensis

12
G47

Brassica oleracea

BH420519

4.00E−43

12
G47

Zinnia elegans

AU292603

5.00E−30

12
G47

Medicago

BE320193

2.00E−24




truncatula


12
G47
Oryza sativa ( indica
AAAA01000718

2.00E−22



cultivar-group)

12
G47

Oryza sativa

AP003379

2.00E−22

12
G47

Oryza sativa

AC124836

1.00E−20



( japonica cultivar-



group)

12
G47

Zea mays

BZ403609

2.00E−20

12
G47

Solanum tuberosum

BQ513932

7.00E−17

12
G47

Pinus taeda

BQ698717

1.00E−16

12
G47

Oryza sativa

gi20161239

8.50E−24



( japonica cultivar-



group)

12
G47

Oryza sativa

gi14140155

8.30E−17

12
G47

Lycopersicon

gi25992102

2.80E−16




esculentum


12
G47

Glycine max

gi31324058

2.80E−16

12
G47

Zea mays

gi21908034

8.60E−15

12
G47

Brassica napus

gi20303011

2.30E−14

12
G47

Atriplex hortensis

gi8571476

3.70E−14

12
G47

Catharanthus

gi8980313

2.60E−13




roseus


12
G47

Hordeum vulgare

gi19071243

5.40E−13

12
G47

Matricaria

gi17385636

1.40E−12




chamomilla


14
G129

Brassica napus

BNABAG1X

1.00E−133

14
G129

Vitis vinifera

CB969483

3.00E−94

14
G129

Silene latifolia

SLSLM1

1.00E−93

14
G129

Panax ginseng

PGORFGAG2

1.00E−92

14
G129

Gossypium

AY083173

3.00E−92




hirsutum


14
G129

Malus x domestica

MDO251118

5.00E−92

14
G129

Corylus avellana

AF027376

2.00E−91

14
G129

Lycopersicon

TOMTAG1A

3.00E−91




esculentum


14
G129

Betula pendula

BPE252071

2.00E−90

14
G129

Nicotiana tabacum

TOBNAG1A

3.00E−90

14
G129

Brassica napus

gi167126

3.30E−125

14
G129

Populus

gi2981131

1.00E−89



balsamifera subsp.




trichocarpa


14
G129

Silene latifolia

gi602900

2.20E−89

14
G129

Panax ginseng

gi3913005

9.30E−89

14
G129

Malus x domestica

gi16973298

2.50E−88

14
G129

Gossypium

gi19743774

2.50E−88




hirsutum


14
G129

Corylus avellana

gi4103757

6.60E−88

14
G129

Lycopersicon

gi3913004

1.70E−87




esculentum


14
G129

Betula pendula

gi8745072

3.60E−87

14
G129

Helianthus annuus

gi27657747

9.60E−87

16
G131

Brassica oleracea

BOU67452

1.00E−139

16
G131

Brassica oleracea

BOL505845

1.00E−139



var. botrytis

16
G131

Sinapis alba

SAAP1

1.00E−137

16
G131

Brassica rapa

BCA251300

1.00E−109



subsp. pekinensis

16
G131

Pisum sativum

PSA279089

4.00E−97

16
G131

Betula pendula

BPMADS3GN

1.00E−95

16
G131

Populus tremuloides

AF034094

3.00E−92

16
G131

Daucus carota

DCA271147

2.00E−90

16
G131

Malus x domestica

MDAJ759

3.00E−90

16
G131

Heuchera

AY306148

2.00E−88




americana


16
G131

Brassica oleracea

gi1561780

6.20E−131

16
G131

Brassica oleracea

gi23304680

6.20E−131



var. botrytis

16
G131

Sinapis alba

gi1076477

3.90E−129

16
G131

Brassica rapa

gi6469345

8.30E−104



subsp. pekinensis

16
G131

Pisum sativum

gi13446154

2.30E−92

16
G131

Betula pendula

gi1483228

2.10E−91

16
G131

Populus tremuloides

gi28381537

4.00E−88

16
G131

Antirrhinum majus

gi16052

3.30E−86

16
G131

Daucus carota

gi22091473

5.30E−86

16
G131

Heuchera

gi32478021

3.40E−84




americana


18
G133

Brassica oleracea

BOU67453

1.00E−125

18
G133

Brassica napus

AF124814

1.00E−118

18
G133

Petunia x hybrida

PHGP

2.00E−74

18
G133

Antirrhinum majus

AJ559554

8.00E−74

18
G133

Nicotiana tabacum

NTMADSBOX

8.00E−73

18
G133

Vitis vinifera

CB971393

1.00E−71

18
G133

Solanum tuberosum

STPD4

5.00E−70

18
G133

Medicago sativa

ALFMBP

1.00E−69

18
G133

Glycine max

AX478039

1.00E−69

18
G133

Chrysanthemum x

AY173060

2.00E−68




morifolium


18
G133

Brassica oleracea

gi1561782

5.30E−118

18
G133

Brassica napus

gi6841082

1.50E−111

18
G133

Brassica oleracea

gi7446540

1.20E−109



var. botrytis

18
G133

Petunia x hybrida

gi22665

4.00E−72

18
G133

Antirrhinum majus

gi100479

1.70E−71

18
G133

Nicotiana tabacum

gi1370276

1.20E−70

18
G133

Solanum tuberosum

gi431226

1.60E−68

18
G133

Medicago sativa

gi1870206

8.90E−68

18
G133

Chrysanthemum x

gi27804367

1.00E−66



morifolium

18
G133

Gerbera hybrida

gi4218171

2.10E−66

20
G134

Cucumis sativus

AF043255

2.00E−67

20
G134

Malus x domestica

MDO291490

2.00E−66

20
G134

Petunia x hybrida

PHFBP3

6.00E−64

20
G134

Vitis vinifera

CB970125

2.00E−62

20
G134

Lactuca sativa

BU013737

3.00E−62

20
G134

Gerbera hybrida

GHY9726

3.00E−62

20
G134

Betula pendula

BPE488589

3.00E−61

20
G134

Eucalyptus grandis

AF029976

6.00E−61

20
G134

Chrysanthemum x

AY173061

1.00E−60




morifolium


20
G134

Silene latifolia

SLSLM2

3.00E−60

20
G134

Cucumis sativus

gi4105097

1.50E−65

20
G134

Malus x domestica

gi12666533

7.40E−64

20
G134

Petunia x hybrida

gi2129971

1.60E−61

20
G134

Gerbera hybrida

gi4218173

6.90E−61

20
G134

Betula pendula

gi28874430

6.20E−60

20
G134

Chrysanthemum x

gi27804369

2.70E−59




morifolium


20
G134

Eucalyptus grandis

gi3114586

5.60E−59

20
G134

Tulipa gesneriana

gi30172225

2.40E−58

20
G134

Silene latifolia

gi602902

2.40E−58

20
G134

Helianthus annuus

gi27657749

3.10E−58

22
G135

Brassica rapa

BCA251300

1.00E−115



subsp. pekinensis

22
G135

Brassica oleracea

BNADBDA

1.00E−109

22
G135

Brassica oleracea

BOL505847

1.00E−109



var. botrytis

22
G135

Sinapis alba

SAAP1

1.00E−107

22
G135

Malus x domestica

MDAJ759

3.00E−83

22
G135

Pisum sativum

PSA279089

3.00E−83

22
G135

Nicotiana tabacum

AF009127

5.00E−81

22
G135

Betula pendula

BPMADS3GN

8.00E−80

22
G135

Nicotiana sylvestris

AF068726

8.00E−80

22
G135

Populus tremuloides

AF034093

2.00E−79

22
G135

Brassica rapa

gi6469345

2.00E−109



subsp. pekinensis

22
G135

Brassica oleracea

gi1561784

1.70E−103

22
G135

Brassica oleracea

gi23304680

9.60E−103



var. botrytis

22
G135

Sinapis alba

gi1076477

1.20E−102

22
G135

Pisum sativum

gi13446154

8.30E−81

22
G135

Nicotiana tabacum

gi4102113

1.80E−78

22
G135

Betula pendula

gi1483228

1.60E−77

22
G135

Populus tremuloides

gi28381535

1.60E−77

22
G135

Nicotiana sylvestris

gi5070144

2.00E−77

22
G135

Daucus carota

gi22091473

3.00E−76

24
G136

Brassica napus

AY036062

1.00E−126

24
G136

Liquidambar

AF103903

1.00E−89



styraciflua

24
G136

Vitis vinifera

AF265562

1.00E−88

24
G136

Rosa rugosa

AB025643

3.00E−88

24
G136

Medicago

CB066648

4.00E−88




truncatula


24
G136

Malus x domestica

MDO251117

6.00E−88

24
G136

Gossypium

BQ411600

4.00E−86




arboreum


24
G136

Nicotiana tabacum

TOBNAG1A

8.00E−84

24
G136

Panax ginseng

PGORFGAG2

2.00E−83

24
G136

Betula pendula

BPE252071

3.00E−83

24
G136

Brassica napus

gi12655901

1.70E−119

24
G136

Liquidambar

gi5031217

9.60E−87




styraciflua


24
G136

Rosa rugosa

gi6970411

3.70E−85

24
G136

Malus x domestica

gi16973296

9.90E−85

24
G136

Vitis vinifera

gi14279306

1.10E−83

24
G136

Nicotiana tabacum

gi3913007

1.30E−80

24
G136

Betula pendula

gi8745072

2.20E−80

24
G136

Panax ginseng

gi3913005

3.60E−80

24
G136

Corylus avellana

gi4103757

5.80E−80

24
G136

Petunia integrifolia

gi848999

5.80E−80

26
G137

Brassica oleracea

BOL508053

1.00E−130



avar. botrytis

26
G137

Malus domestica

U78947

1.00E−105

26
G137

Malus x domestica

MDAJ1681

1.00E−104

26
G137

Cucumis sativus

AF135962

1.00E−102

26
G137

Vitis vinifera

AF373601

1.00E−100

26
G137

Populus tremuloides

AF185574

1.00E−100

26
G137

Fragaria x

AF484683

6.00E−93




ananassa


26
G137

Gossypium

BG440326

2.00E−90




arboreum


26
G137

Prunus persica

BU048398

4.00E−89

26
G137

Antirrhinum majus

AMDEFH49G

2.00E−87

26
G137

Brassica oleracea

gi23304688

2.10E−123



var. botrytis

26
G137

Malus x domestica

gi3290209

7.70E−101

26
G137

Malus domestica

gi7488622

7.70E−101

26
G137

Cucumis sativus

gi6683777

1.70E−98

26
G137

Populus tremuloides

gi28372802

1.30E−96

26
G137

Vitis vinifera

gi20385584

1.70E−96

26
G137
Fragaria x
gi28628841

1.90E−90




ananassa


26
G137

Antirrhinum majus

gi1239961

2.90E−85

26
G137

Lycopersicon

gi24967143

1.10E−83




esculentum


26
G137

Petunia x hybrida

gi13384048

3.00E−83

28
G138

Brassica oleracea

BOL508052

1.00E−114



var. botrytis

28
G138

Petunia x hybrida

AF335236

2.00E−72

28
G138

Lycopersicon

AY294329

3.00E−72




esculentum


28
G138

Nicotiana tabacum

AF068723

5.00E−72

28
G138

Capsicum annuum

AF129875

1.00E−70

28
G138

Malus domestica

U78947

3.00E−70

28
G138

Malus x domestica

MDAJ1681

1.00E−69

28
G138

Pinus radiata

PRU42399

2.00E−69

28
G138

Antirrhinum majus

AMDEFH49G

2.00E−69

28
G138

Daucus carota

DCA271151

8.00E−69

28
G138

Brassica oleracea

gi23304686

4.20E−109



var. botrytis

28
G138

Petunia x hybrida

gi13384050

5.90E−71

28
G138

Nicotiana tabacum

gi8567991

1.20E−70

28
G138

Lycopersicon

gi31747208

2.60E−70




esculentum


28
G138

Capsicum annuum

gi6651033

4.80E−69

28
G138

Antirrhinum majus

gi1239961

2.10E−68

28
G138

Malus x domestica

gi3290209

4.30E−68

28
G138

Malus domestica

gi7488622

4.30E−68

28
G138

Daucus carota

gi22091481

8.90E−68

28
G138

Pinus radiata

gi1206003

1.50E−67

30
G139

Brassica oleracea

BOL508053

1.00E−115




var. botrytis


30
G139

Populus tremuloides

AF185574

1.00E−100

30
G139

Cucumis sativus

AF135962

4.00E−97

30
G139

Malus domestica

U78947

2.00E−96

30
G139

Malus x domestica

MDAJ1681

5.00E−96

30
G139

Vitis vinifera

AF373601

9.00E−92

30
G139

Populus

BU869391

9.00E−89



balsamifera subsp.




trichocarpa


30
G139

Fragaria x

AF484683

8.00E−88




ananassa


30
G139
Prunus persica
BU048398

1.00E−86

30
G139

Antirrhinum majus

AMDEFH49G

8.00E−85

30
G139

Brassica oleracea

gi23304688

6.00E−110



var. botrytis

30
G139

Populus tremuloides

gi28372802

2.20E−96

30
G139

Cucumis sativus

gi6683777

2.00E−93

30
G139

Malus x domestica

gi3290209

2.60E−93

30
G139

Malus domestica

gi7488622

2.60E−93

30
G139

Vitis vinifera

gi20385584

1.00E−89

30
G139

Fragaria x

gi28628841

6.80E−86




ananassa


30
G139

Antirrhinum majus

gi1239961

1.30E−82

30
G139

Petunia x hybrida

gi13384048

2.00E−79

30
G139

Lycopersicon

gi24967143

2.30E−78




esculentum


32
G140

Brassica napus

CD818823

1.00E−112

32
G140

Liquidambar

AF103903

7.00E−88




styraciflua


32
G140

Vitis vinifera

AF265562

9.00E−88

32
G140

Medicago

CB066648

2.00E−87




truncatula


32
G140

Gossypium

BQ411600

2.00E−85




arboreum


32
G140

Malus x domestica

MDO251117

4.00E−83

32
G140

Rosa rugosa

AB025643

5.00E−83

32
G140

Nicotiana tabacum

TOBNAG1A

1.00E−80

32
G140

Petunia integrifolia

PETFHPP

1.00E−80

32
G140

Citrus sinensis

CB290594

2.00E−80

32
G140

Brassica napus

gi12655901

4.10E−102

32
G140

Liquidambar

gi5031217

6.10E−85



styraciflua

32
G140

Vitis vinifera

gi14279306

8.00E−83

32
G140

Rosa rugosa

gi6970411

3.10E−81

32
G140

Malus x domestica

gi16973296

4.60E−80

32
G140

Nicotiana tabacum

gi3913007

3.70E−78

32
G140

Petunia integrifolia

gi848999

2.00E−77

32
G140

Petunia x hybrida

gi2129972

5.40E−77

32
G140

Panax ginseng

gi3913005

5.40E−77

32
G140

Lycopersicon

gi3913004

8.80E−77




esculentum


34
G142

Brassica oleracea

BOL508409

1.00E−127



var. botrytis

34
G142

Vitis vinifera

AF373602

1.00E−88

34
G142

Malus domestica

MDAJ763

3.00E−84

34
G142

Petunia x hybrida

AB031035

2.00E−77

34
G142

Agapanthus praecox

AB079261

1.00E−76

34
G142

Chrysanthemum x

AY173062

8.00E−75




morifolium


34
G142

Oryza sativa

OSU78782

6.00E−74

34
G142

Oryza sativa

AK069103

6.00E−74



( japonica cultivar-



group)

34
G142

Zea mays

MZEMADSB

3.00E−73

34
G142

Triticum aestivum

AB007505

3.00E−72

34
G142

Brassica oleracea

gi23304710

6.50E−120



var. botrytis

34
G142

Vitis vinifera

gi20385586

3.30E−86

34
G142

Malus domestica

gi3646340

1.20E−81

34
G142

Petunia x hybrida

gi7544096

1.60E−75

34
G142

Agapanthus praecox

gi29467050

1.50E−74

34
G142

Oryza sativa

gi2286109

2.20E−73

34
G142

Chrysanthemum x

gi27804371

4.50E−73




morifolium


34
G142

Zea mays

gi7446515

1.50E−72

34
G142

Lolium perenne

gi28630959

8.40E−72

34
G142

Triticum aestivum

gi3688591

2.20E−71

36
G145

Sinapis alba

SAMADSD

1.00E−127

36
G145

Vitis vinifera

CB980340

5.00E−98

36
G145

Lycopersicon

AY294330

5.00E−97




esculentum


36
G145

Nicotiana sylvestris

AF068722

8.00E−96

36
G145

Pisum sativum

PSAJ3318

1.00E−95

36
G145

Brassica napus

CD814473

2.00E−95

36
G145

Petunia x hybrida

PETTRNSFB

2.00E−95

36
G145

Antirrhinum majus

AMDEFH200

3.00E−95

36
G145

Gossypium

AF538965

5.00E−95




hirsutum


36
G145

Populus tremuloides

AY235222

7.00E−95

36
G145

Sinapis alba

gi1617211

7.30E−121

36
G145

Vitis vinifera

gi20385588

6.00E−94

36
G145

Lycopersicon

gi31747210

2.00E−93




esculentum


36
G145

Pisum sativum

gi3184054

6.90E−93

36
G145

Nicotiana sylvestris

gi5070138

2.30E−92

36
G145

Petunia x hybrida

gi1181186

4.80E−92

36
G145

Populus tremuloides

gi30314024

7.90E−92

36
G145

Antirrhinum majus

gi1239959

1.60E−91

36
G145

Gossypium

gi23194451

2.10E−91




hirsutum


36
G145

Chrysanthemum x

gi27804361

1.50E−88




morifolium


38
G146

Brassica napus

CD818636

1.00E−104

38
G146

Gossypium

AF538966

1.00E−91




hirsutum


38
G146

Gossypium

BG441292

1.00E−90




arboreum


38
G146

Medicago

BI311053

3.00E−89




truncatula


38
G146

Cucumis sativus

AF022377

4.00E−89

38
G146

Vitis vinifera

CB975703

1.00E−88

38
G146

Momordica

AY178837

1.00E−87




charantia


38
G146

Glycine max

AW184799

2.00E−82

38
G146

Phaseolus

CA902463

5.00E−79




coccineus


38
G146

Malus domestica

MDAJ762

7.00E−79

38
G146

Gossypium

gi23194453

8.40E−88




hirsutum


38
G146

Cucumis sativus

gi4103342

1.40E−85

38
G146

Vitis vinifera

gi20385590

2.60E−84

38
G146

Momordica

gi27763670

8.90E−84




charantia


38
G146

Agapanthus praecox

gi29467048

1.30E−75

38
G146

Petunia x hybrida

gi1568513

1.00E−73

38
G146

Hyacinthus

gi21955182

6.60E−72




orientalis


38
G146

Panax ginseng

gi3913005

7.50E−71

38
G146

Lycopersicon

gi24967137

1.60E−70




esculentum


38
G146

Populus

gi2981133

8.70E−70



balsamifera subsp.




trichocarpa


39
G148

Glycine max

GLYMA-28NOV01-
704




CLUSTER24877_1

39
G148

Glycine max

GLYMA-28NOV01-
705




CLUSTER99362_1

39
G148

Oryza sativa

ORYSA-22JAN02-
706




CLUSTER865_1

39
G148

Oryza sativa

OSC101589.C1.p14.fg
707

39
G148

Zea mays

L1B4766-083-R1-K1-A9
708

39
G148

Zea mays

ZEAMA-08NOV01-
709




CLUSTER914_1

39
G148

Zea mays

ZEAMA-08NOV01-
710




CLUSTER914_14

39
G148

Zea mays

ZEAMA-O8NOV01-
711




CLUSTER914_2

39
G148

Zea mays

ZEAMA-O8NOV01-
712




CLUSTER914_3

39
G148

Oryza sativa

Os_S31752
1560

39
G148

Oryza sativa

Os_S63871
1561

39
G148

Oryza sativa

Os_S65486
1562

39
G148

Zea mays

Zm_S11418374
1755


39
G148

Zea mays

Zm_S11418375
1756

39
G148

Triticum aestivum

Ta_S66204
1835

39
G148

Lycopersicon

SGN-UNIGENE−44128
1943




esculentum


39
G148

Lycopersicon

SGN-UNIGENE−
1944




esculentum

SINGLET-42436

40
G148

Brassica oleracea

BOL508409

3.00E−74



var. botrytis

40
G148

Malus domestica

MDAJ763

2.00E−65

40
G148

Vitis vinifera

AF373602

3.00E−64

40
G148

Petunia x hybrida

AB031035

1.00E−59

40
G148

Chrysanthemum x

AY173062

1.00E−58



morifolium

40
G148

Oryza sativa

OSU78782

3.00E−57

40
G148

Oryza sativa

AK069103

3.00E−57



( japonica cultivar-



group)

40
G148

Triticum aestivum

AB007505

1.00E−56

40
G148

Lolium perenne

AY198329

1.00E−55

40
G148

Poa annua

AF372840

5.00E−55

40
G148

Brassica oleracea

gi23304710

1.70E−73



var. botrytis

40
G148

Malus domestica

gi3646340

6.50E−65

40
G148

Vitis vinifera

gi20385586

7.40E−64

40
G148

Petunia x hybrida

gi7544096

7.10E−59

40
G148

Chrysanthemum x

gi27804371

2.20E−57




morifolium


40
G148

Triticum aestivum

gi3688591

3.50E−57

40
G148

Oryza sativa

gi2286109

4.50E−57

40
G148

Lolium perenne

gi28630959

5.20E−56

40
G148

Poa annua

gi13958339

8.40E−56

40
G148

Agapanthus praecox

gi29467050

9.70E−55

42
G151

Brassica napus

BNU22681

1.00E−94

42
G151

Selaginella

AB086021

5.00E−39




remotifolia


42
G151

Lycopodium

AF425598

5.00E−37




annotinum


42
G151

Helianthus annuus

BQ970680

2.00E−36

42
G151

Pinus radiata

PRU42400

9.00E−36

42
G151

Gnetum parvifolium

AB022665

1.00E−35

42
G151

Gnetum gnemon

GGN132215

1.00E−35

42
G151

Pinus resinosa

PRMADS1

2.00E−35

42
G151

Oryza sativa

AY177696

6.00E−35



( japonica cultivar-



group

42
G151

Oryza sativa

OSU78782

8.00E−35

42
G151

Brassica napus

gi3831486

9.40E−96

42
G151

Selaginella

gi29467138

2.20E−39




remotifolia


42
G151

Lycopodium

gi21396795

1.80E−37




annotinum


42
G151

Pinus radiata

gi1206005

1.60E−36

42
G151

Gnetum gnemon

gi5019456

2.60E−36

42
G151

Gnetum parvifolium

gi6092009

2.60E−36

42
G151

Pinus resinosa

gi1702951

3.40E−36

42
G151

Oryza sativa

gi2286109

5.50E−36

42
G151

Zea mays

gi7446514

5.50E−36

42
G151

Lolium perenne

gi28630959

1.10E−35

43
G153

Oryza sativa

G3479
2189
2.00E−59

43
G153

Glycine max

G3484
2191
3.00E−77

43
G153

Glycine max

G3485
2193
9.00E−63

43
G153

Zea mays

G3487
2195
5.00E−63

43
G153

Zea mays

G3488
2197
2.00E−61

43
G153

Zea mays

G3489
2199
6.00E−66

43
G153

Glycine max

GLYMA-28NOV01-
713




CLUSTER393266_1

43
G153

Glycine max

GLYMA-28NOV01-
714




CLUSTER84992_1

43
G153

Oryza sativa

OSC19180.C1.p14.fg
715

43
G153

Zea mays

ZEAMA-O8NOV01-
716




CLUSTER124_1

43
G153

Zea mays

ZEAMA-O8NOV01-
717




CLUSTER226078_2

43
G153

Zea mays

uC_zmf1Mo17202h01
718

43
G153

Glycine max

Gma_S5139103
1634

43
G153

Zea mays

Zm_S11418691
1757

43
G153

Zea mays

Zm_S11433900
1758

43
G153

Lycopersicon

SGN-UNIGENE-
1945




esculentum

SINGLET-362903

43
G153

Lycopersicon

SGN-UNIGENE-
1946




esculentum

SINGLET-8562

44
G153

Antirrhinum majus

AMDEFH125

1.00E−67

44
G153

Zea mays

AF112149

8.00E−63

44
G153

Oryza sativa

AY177696

1.00E−62



( japonica cultivar-



group)

44
G153

Glycine max

AW706936

5.00E−59

44
G153

Medicago

BQ164807

5.00E−59




truncatula


44
G153

Lycopersicon

AW218280

5.00E−56




esculentum


44
G153

Solanum tuberosum

BM405213

2.00E−55

44
G153

Medicago sativa

MSU91964

6.00E−54

44
G153

Triticum aestivum

AX658813

3.00E−49

44
G153

Mesembryanthemum

BE034403

3.00E−48




crystallinum


44
G153

Antirrhinum majus

gi1816459

2.10E−66

44
G153

Oryza sativa

gi30313677

2.90E−62



( japonica cultivar-



group)

44
G153

Zea mays

gi29611976

7.70E−62

44
G153

Medicago sativa

gi1928874

1.30E−52

44
G153

Ipomoea batatas

gi15081463

6.90E−45

44
G153

Oryza sativa

gi7592642

9.10E−43

44
G153

Lolium perenne

gi28630953

8.20E−42

44
G153

Lolium temulentum

gi4204232

1.70E−41

44
G153

Triticum aestivum

gi30721847

2.80E−41

44
G153

Hordeum vulgare

gi9367313

2.80E−41

45
G155

Glycine max

GLYMA-28NOV01-
719




CLUSTER112511_1

45
G155

Glycine max

GLYMA-28NOV01-
720




CLUSTER2290_5

45
G155

Glycine max

GLYMA-28NOV01-
721




CLUSTER24178_1

45
G155

Glycine max

GLYMA-28NOV01-
722




CLUSTER24178_4

45
G155

Glycine max

GLYMA-28NOV01-
723




CLUSTER862_1

45
G155

Glycine max

GLYMA-28NOV01-
724




CLUSTER862_2

45
G155

Glycine max

GLYMA-28NOV01-
725




CLUSTER862_3

45
G155

Glycine max

LIB4127-088-Q1-N1-F3
726

45
G155

Oryza sativa

ORYSA-22JAN02-
727




CLUSTER117_2

45
G155

Oryza sativa

ORYSA-22JAN02-
728




CLUSTER397_1

45
G155

Oryza sativa

OSC101428.C1.p6.fg
729

45
G155

Oryza sativa

OSC22851.C1.p5.fg
730

45
G155

Oryza sativa

OSC23163.C1.p8.fg
731

45
G155

Zea mays

ZEAMA-08NOV01-
732




CLUSTER444_10

45
G155

Zea mays

ZEAMA-08NOV01-
733




CLUSTER444_17

45
G155

Zea mays

ZEAMA-08NOV01-
734




CLUSTER444_19

45
G155

Zea mays

ZEAMA-08NOV01-
735




CLUSTER444_20

45
G155

Zea mays

ZEAMA-08NOV01-
736




CLUSTER444_25

45
G155

Zea mays

ZEAMA-08NOV01-
737




CLUSTER444_29

45
G155

Zea mays

ZEAMA-08NOV01-
738




CLUSTER444_38

45
G155

Zea mays

ZEAMA-08NOV01-
739




CLUSTER49_1

45
G155

Zea mays

ZEAMA-08NOV01-
740




CLUSTER49_2

45
G155

Oryza sativa

Os_S60917
1563

45
G155

Oryza sativa

Os_S65478
1564

45
G155

Oryza sativa

Os_S65480
1565

45
G155

Oryza sativa

Os_S81123
1566

45
G155

Glycine max

Gma_S5001655
1635

45
G155

Hordeum vulgare

Hv_S74097
1719

45
G155

Hordeum vulgare

Hv_S74098
1720

45
G155

Hordeum vulgare

Hv_S74100
1721

45
G155

Zea mays

Zm_S11355421
1759

45
G155

Zea mays

Zm_S11374058
1760

45
G155

Zea mays

Zm_S11418377
1761

45
G155

Zea mays

Zm_S11418747
1762

45
G155

Zea mays

Zm_S11435147
1763

45
G155

Zea mays

Zm_S11527666
1764

45
G155

Triticum aestivum

Ta_S185481
1836

45
G155

Triticum aestivum

Ta_S210240
1837

45
G155

Triticum aestivum

Ta_S66203
1838

45
G155

Lycopersicon

Les_S5931544
1926




esculentum


45
G155

Lycopersicon

SGN-UNIGENE-44980
1947




esculentum


45
G155

Lycopersicon

SGN-UNIGENE-45332
1948




esculentum


45
G155

Lycopersicon

SGN-UNIGENE-
1949




esculentum

SINGLET-329711

45
G155

Lycopersicon

SGN-UNIGENE-
1950




esculentum

SINGLET-457556

45
G155

Lycopersicon

SGN-UNIGENE-
1951




esculentum

SINGLET-477293

45
G155

Lycopersicon

SGN-UNIGENE-
1952




esculentum

SINGLET-67345

46
G155

Brassica oleracea

BOL505844

1.00E−123



var. botrytis

46
G155

Sinapis alba

SAU25695

1.00E−120

46
G155

Brassica napus

GD841921

1.00E−119

46
G155

Betula pendula

BPMADS5GN

3.00E−82

46
G155

Nicotiana sylvestris

AF068725

1.00E−79

46
G155

Nicotiana tabacum

AF385746

2.00E−79

46
G155

Malus x domestica

MDU78948

3.00E−78

46
G155

Capsicum annuum

AF130118

4.00E−78

46
G155

Petunia x hybrida

AF176783

1.00E−77

46
G155

Lycopersicon

AW442282

1.00E−77




esculentum


46
G155

Brassica oleracea

gi23304678

2.00E−116



var. botrytis

46
G155

Sinapis alba

gi1049024

2.40E−113

46
G155

Betula pendula

gi1483232

2.00E−79

46
G155

Nicotiana sylvestris

gi5070142

5.40E−77

46
G155

Nicotiana tabacum

gi27373049

8.80E−77

46
G155

Capsicum annuum

gi14518447

7.90E−76

46
G155

Petunia x hybrida

gi6606306

1.30E−75

46
G155

Malus x domestica

gi3947985

3.40E−75

46
G155
Petunia sp.
gi6731756

7.10E−75

46
G155

Lycopersicon

gi23428887

1.90E−74




esculentum


48
G171

Brassica oleracea

BZ059285

2.00E−86

48
G171

Lotus japonicus

AG231874

1.00E−15

48
G171

Glycine max

BE610209

1.00E−11

48
G171

Medicago

AC135316

3.00E−10




truncatula


48
G171

Zea mays

GC654475

6.00E−10

48
G171
Oryza sativa ( indica
AAAA01000422

1.00E−09



cultivar-group)

48
G171

Oryza sativa

AP002480

1.00E−09

48
G171

Vitis vinifera

CB980345

2.00E−09

48
G171

Oryza sativa

AP006531

9.00E−08



( japonica cultivar-



group)

48
G171

Lycopersicon

BI930829

2.00E−07




esculentum


48
G171

Oryza sativa

gi8096379

4.10E−11

48
G171

Oryza sativa

gi15623935

6.90E−09



( japonica cultivar-



group)

48
G171

Malus x domestica

gi32452884

5.60E−07

48
G171

Petunia x hybrida

gi13384058

5.60E−07

48
G171

Lycopodium

gi30525823

8.10E−07




annotinum


48
G171

Ceratopteris

gi2252482

1.40E−06




richardii


48
G171

Cichorium intybus

gi3986689

3.00E−06

48
G171

Brassica napus

gi3831486

3.60E−06

48
G171

Ipomoea nil

gi27372827

3.80E−06

48
G171

Rosa rugosa

gi9857312

5.30E−06

50
G172

Brassica oleracea

BH583694

9.00E−52

50
G172

Medicago

AC144481

5.00E−09




truncatula


50
G172
Oryza sativa ( indica
AAAA01005416

3.00E−08



cultivar-group)

50
G172

Oryza sativa

AC098572

3.00E−08

50
G172

Lotus japonicus

AP006142

5.00E−08

50
G172

Lotus corniculatus

AP006379

1.00E−06



var. japonicus

50
G172

Lycopersicon

BH014401

9.00E−06




esculentum


50
G172

Zea mays

CC690653

2.00E−05

50
G172

Oryza sativa

AP004784

4.00E−05



( japonica cultivar-



group)

50
G172

Sorghum bicolor

BZ627051

6.00E−05

50
G172

Brassica rapa

gi30523366

5.60E−06

50
G172

Brassica napus

gi17933454

1.00E−05

50
G172

Brassica oleracea

gi30523252

1.00E−05



var. capitata

50
G172

Zea mays

gi1076827

1.10E−05

50
G172

Oryza sativa

gi30313673

2.10E−05



( japonica cultivar-



group)

50
G172

Lolium perenne

gi28630955

6.40E−05

50
G172

Lolium temulentum

gi4204234

6.40E−05

50
G172

Oryza sativa

gi15290141

7.10E−05

50
G172

Raphanus sativus

gi30523250

0.0001

50
G172

Cucumis sativus

gi8216957

0.00014

52
G173

Brassica oleracea

BZ519961

3.00E−20

52
G173

Zea mays

AX540653

8.00E−17

52
G173
Oryza sativa ( indica
AAAA01022896

1.00E−07



cultivar-group)

52
G173

Oryza sativa

AC093312

5.00E−07

52
G173

Medicago

AC135316

3.00E−06




truncatula


52
G173

Glycine max

AW508033

1.00E−05

52
G173

Physcomitrella

PPA419330

3.00E−05




patens


52
G173

Oryza sativa

AP005734

2.00E−04



( japonica cultivar-



group)

52
G173

Triticum aestivum

BJ303010

7.00E−04

52
G173

Lotus japonicus

AG231874

0.001

52
G173

Physcomitrella

gi22474466

1.30E−08




patens


52
G173

Ipomoea batatas

gi13448658

1.40E−08

52
G173

Oryza sativa

gi8096379

1.90E−08

52
G173

Magnolia

gi16549058

1.40E−06




praecocissima


52
G173

Sinapis alba

gi1617211

1.90E−06

52
G173

Gnetum gnemon

gi27151621

1.90E−06

52
G173

Physcomitrella

gi22090622

3.00E−06



patens subsp. patens

52
G173

Cichorium intybus

gi3986689

3.30E−06

52
G173

Nicotiana sylvestris

gi5070144

4.70E−06

52
G173

Papaver nudicaule

gi3170500

4.70E−06

53
G200

Glycine max

GLYMA-28NOV01-
741




CLUSTER46239_2

53
G200

Oryza sativa

1945280
742

53
G200

Oryza sativa

OSC1073.C1.p11.fg
743

53
G200

Oryza sativa

rsicem_4884.y1.abd
744

53
G200

Zea mays

LIB4980-049-R1-K1-C12
745

53
G200

Zea mays

ZEAMA-08NOV01-
746




CLUSTER286_1

53
G200

Oryza sativa

Os_S60479
1567

53
G200

Medicago

Mtr_S5340749
1691




truncatula


53
G200

Zea mays

Zm_S11327053
1765

53
G200

Zea mays

Zm_S11454145
1766

53
G200

Zea mays

Zm_S11529138
1767

53
G200

Zea mays

Zm_S11529143
1768

53
G200

Zea mays

Zm_S11529165
1769

53
G200

Lycopersicon

SGN-UNIGENE-57276
1953




esculentum


53
G200

Lycopersicon

SGN-UNIGENE-
1954




esculentum

SINGLET-385670

54
G200

Populus

BU876112

8.00E−79



balsamifera subsp.




trichocarpa


54
G200

Beta vulgaris

BQ587622

8.00E−73

54
G200

Gossypium

BG441590

6.00E−70




arboreum


54
G200

Oryza sativa

AK061437

1.00E−68



( japonica cultivar-



group)

54
G200

Oryza sativa

OSMYB1308

1.00E−68

54
G200

Poncirus trifoliata

CD576612

3.00E−68

54
G200

Nuphar advena

CD474802

2.00E−66

54
G200

Brassica oleracea

BH976962

4.00E−66

54
G200

Lycopersicon

LETHM6

5.00E−65




esculentum


54
G200

Zea mays

AY107969

5.00E−65

54
G200

Oryza sativa

gi1945281

5.80E−68

54
G200

Antirrhinum majus

gi256828

6.00E−62

54
G200

Oryza sativa

gi33087065

7.50E−62



( japonica cultivar-



group)

54
G200

Lycopersicon

gi1430848

1.00E−59




esculentum


54
G200

Zea mays

gi19072744

8.40E−56

54
G200
Dendrobium sp.
gi28628947

1.60E−53



XMW-2002-1

54
G200

Lotus corniculatus

gi30024598

5.90E−50



var. japonicus

54
G200

Petunia x hybrida

gi20561

1.20E−49

54
G200

Nicotiana tabacum

gi6552389

2.40E−49

54
G200

Glycine max

gi5139802

8.60E−47

56
G224

Brassica oleracea

BH442040

2.00E−38

56
G224
Oryza sativa ( indica
AAAA01016190

4.00E−08



cultivar-group)

56
G224

Oryza sativa

AP005575

5.00E−08



( japonica cultivar-



group)

56
G224

Lotus japonicus

AP004985

9.00E−07

56
G224

Medicago

AC135101

2.00E−06




truncatula


56
G224

Prunus persica

BU040633

6.00E−06

56
G224

Brassica rapa

BQ791286

2.00E−05



subsp. pekinensis

56
G224

Oryza sativa

OSJN00182

2.00E−05

56
G224

Brassica napus

CD832269

2.00E−05

56
G224

Vitis vinifera

CB345908

5.00E−05

56
G224

Oryza sativa

gi8096405

1.40E−06

56
G224

Antirrhinum

gi13161528

3.60E−06




hispanicum


56
G224

Oryza sativa

gi31433564

8.80E−06



( japonica cultivar-



group)

56
G224

Prunus mume

gi29420809

0.0019

56
G224

Prunus dulcis

gi28866899

0.014

56
G224

Lycopersicon

gi9858770

0.91




esculentum


56
G224

Daucus carota

gi20066308

0.99

56
G224

Lotus japonicus

gi28624856

1

58
G244

Brassica oleracea

BH667251

1.00E−120

58
G244

Oryza sativa

AX755614

5.00E−88

58
G244

Hordeum vulgare

BI959020

6.00E−88

58
G244
Oryza sativa ( indica
AAAA01009672

7.00E−79



cultivar-group)

58
G244

Sorghum bicolor

AF474126

6.00E−69

58
G244

Vitis vinifera

CB971781

8.00E−68

58
G244

Lycopersicon

AW624307

3.00E−54




esculentum


58
G244

Solanum tuberosum

BG592600

3.00E−53

58
G244

Mesembryanthemum

BG269414

1.00E−52




crystallinum


58
G244

Populus x

AY129246

2.00E−52




canescens


58
G244

Oryza sativa

gi32487951

2.60E−90



( japonica cultivar-



group)

58
G244

Sorghum bicolor

gi19073322

7.20E−66

58
G244

Oryza sativa

gi1946267

1.20E−55

58
G244

Populus x

gi22795039

3.40E−52




canescens


58
G244

Gossypium

gi13346188

4.30E−52




hirsutum


58
G244

Antirrhinum majus

gi485867

3.20E−51

58
G244

Lotus corniculatus

gi30024600

8.00E−51



var. japonicus

58
G244

Petunia x hybrida

gi20563

1.70E−50

58
G244

Zea mays

gi127582

3.50E−50

58
G244

Boea crassifolia

gi30575840

5.70E−50

60
G246

Lycopersicon

AW624217

3.00E−36




esculentum


60
G246

Brassica napus

CD841933

1.00E−35

60
G246

Oryza sativa

AK107461

1.00E−34



( japonica cultivar-



group)

60
G246

Solanum tuberosum

BQ514458

2.00E−34

60
G246

Oryza sativa

AX699697

2.00E−33

60
G246

Physcomitrella

AX288143

6.00E−33




patens


60
G246

Medicago

BQ165917

3.00E−32




truncatula


60
G246

Zinnia elegans

AU287803

4.00E−32

60
G246

Citrus sinensis

BQ623005

5.00E−32

60
G246

Populus tremula x

POP567345

1.00E−31




Populus tremuloides


60
G246

Oryza sativa

gi6539552

1.50E−35

60
G246

Populus tremula x

gi31980093

8.30E−33




Populus tremuloides


60
G246

Oryza sativa

gi21321780

3.60E−32



( japonica cultivar-



group)

60
G246

Solanum tuberosum

gi9954112

1.00E−25

60
G246

Nicotiana tabacum

gi27529846

2.20E−25

60
G246

Papaver rhoeas

gi7230673

1.10E−22

60
G246

Physcomitrella

gi8745321

1.30E−22




patens


60
G246

Adiantum

gi7677136

3.30E−22




raddianum


60
G246

Hordeum vulgare

gi8745325

1.80E−21

60
G246

Secale cereale

gi7677132

3.80E−21

62
G253

Brassica napus

GD841933

3.00E−26

62
G253

Lycopersicon

AW624217

3.00E−26




esculentum


62
G253

Solanum tuberosum

BQ514458

2.00E−25

62
G253

Medicago

BQ165917

4.00E−25




truncatula


62
G253

Citrus sinensis

BQ623005

6.00E−25

62
G253

Triticum aestivum

BQ838360

2.00E−24

62
G253

Oryza sativa

AK107461

2.00E−24



( japonica cultivar-



group)

62
G253

Physcomitrella

AX288143

4.00E−24




patens


62
G253

Zinnia elegans

AU287803

8.00E−24

62
G253

Papaver rhoeas

AF236059

2.00E−23

62
G253

Oryza sativa

gi21321780

1.50E−28



( japonica cultivar-



group)

62
G253

Oryza sativa

gi6979341

7.10E−27

62
G253

Papaver rhoeas

gi7230673

3.20E−24

62
G253

Populus tremula x

gi31980093

4.10E−24




Populus tremuloides


62
G253

Adiantum

gi7677136

9.70E−23




raddianum


62
G253

Solanum tuberosum

gi9954114

1.10E−22

62
G253

Nicotiana tabacum

gi16326137

2.40E−22

62
G253

Physcomitrella

gi8745321

2.00E−21




patens


62
G253

Craterostigma

gi1002800

7.80E−21




plantagineum


62
G253

Hordeum vulgare

gi8745325

1.00E−20

64
G268

Oryza sativa

AK070193

1.0e−999



( japonica cultivar-



group)

64
G268

Zea mays

AY104480

1.00E−146

64
G268

Oryza sativa

AX654858

1.00E−125

64
G268

Malus domestica

AF220204

1.00E−116

64
G268

Glycine max

CA785038

1.00E−112

64
G268

Solanum tuberosum

BM406566

1.00E−111

64
G268

Lactuca sativa

BQ863563

1.00E−101

64
G268

Lycopersicon

BI936015

1.00E−101




esculentum


64
G268

Medicago

BE203572

1.00E−100




truncatula


64
G268
Oryza sativa ( indica
CB621547

1.00E−100



cultivar-group)

64
G268

Oryza sativa

gi15408875

4.90E−250

64
G268

Oryza sativa

gi20160625

4.90E−250



( japonica cultivar-



group)

64
G268

Malus x domestica

gi6752888

2.40E−113

64
G268

Narcissus

gi18419598

8.90E−52




pseudonarcissus


64
G268

Pinus pinaster

gi20218829

1.70E−09

64
G268

Nicotiana alata

gi1247388

0.00015

64
G268

Bromheadia

gi2108256

0.00025




finlaysoniana


64
G268

Lycopersicon

gi1345539

0.00031




esculentum


64
G268

Antirrhinum majus

gi16029

0.00064

64
G268

Volvox carteri

gi21992

0.0024

66
G287

Vicia faba

VFPTF2

5.00E−99

66
G287

Oryza sativa

AK069464

1.00E−80



( japonica cultivar-



group)

66
G287

Brassica oleracea

BZ074994

6.00E−63

66
G287

Lactuca sativa

BQ869065

1.00E−61

66
G287
Oryza sativa ( indica
CB620939

1.00E−58



cultivar-group)

66
G287

Amborella

CD482217

2.00E−52




trichopoda


66
G287

Solanum tuberosum

BG599712

1.00E−40

66
G287

Medicago

BG648535

2.00E−32




truncatula


66
G287

Triticum aestivum

CD897359

1.00E−29

66
G287

Oryza sativa

AP002536

2.00E−14

66
G287

Vicia faba

gi2104683

5.80E−99

66
G287

Oryza sativa

gi28301944

3.90E−09



( japonica cultivar-



group)

66
G287

Oryza sativa

gi15451572

0.004

66
G287

Lycopersicon

gi13620220

0.47




esculentum


66
G287

Brassica nigra

gi20148766

0.47

66
G287

Nicotiana tabacum

gi119714

0.57

66
G287

Spermatozopsis

gi4584086

0.75




similis


66
G287

Prunus armeniaca

gi2688826

0.99

66
G287

Petunia x hybrida

gi21105740

1

68
G309

Gossypium

AY208992

1.00E−180




hirsutum


68
G309

Vitis vinifera

AF378125

1.00E−176

68
G309

Lycopersicon

AY26908
7
1.00E−175




esculentum


68
G309

Brassica napus

AX081276

1.00E−164

68
G309

Zea mays

ZMA242530

1.00E−158

68
G309

Hordeum vulgare

AF460219

1.00E−157

68
G309

Triticum aestivum

BD074479

1.00E−157

68
G309
Oryza sativa ( indica
AAAA01015042

1.00E−137



cultivar-group)

68
G309

Oryza sativa

AC087797

1.00E−137

68
G309
Zea mays subsp.
AF413202

1.00E−137




mays


68
G309

Vitis vinifera

gi20334379

2.10E−170

68
G309

Calycadenia

gi20257451

1.10E−155




multiglandulosa


68
G309

Brassica napus

gi13170126

2.30E−155

68
G309

Hordeum vulgare

gi18254373

5.30E−153

68
G309

Triticum aestivum

gi5640157

1.10E−152

68
G309

Cariquistia muirii

gi20257447

1.20E−151

68
G309

Argyroxiphium

gi20257461

1.50E−151




kauense


68
G309

Madia sativa

gi20257442

5.00E−151

68
G309

Argyroxiphium

gi20257457

5.00E−151



sandwicense subsp.




macrocephalum


68
G309

Dubautia

gi20257473

1.30E−150




raillardioides


70
G314

Lotus japonicus

AP006085

7.00E−94

70
G314

Medicago

AC137703

1.00E−90




truncatula


70
G314

Oryza sativa

AP003823

8.00E−82

70
G314
Oryza sativa ( indica
AAAA01000614

8.00E−82



cultivar-group)

70
G314

Oryza sativa

AP005149

8.00E−82



( japonica cultivar-



group)

70
G314

Brassica oleracea

BZ432637

1.00E−72

70
G314

Solanum tuberosum

BF052580

1.00E−47

70
G314

Lycopersicon

BG123921

2.00E−47




esculentum


70
G314

Glycine max

BH152995

2.00E−35

70
G314

Zea mays

CC732802

4.00E−22

70
G314

Oryza sativa

gi28564823

7.40E−80



( japonica cultivar-



group)

70
G314

Oryza sativa

gi14719333

1.30E−26

70
G314

Lycopersicon

gi13620224

1.60E−25




esculentum


70
G314

Brassica napus

gi13170126

2.00E−19

70
G314
Zea mays subsp.
gi15866286

7.40E−18




mays


71
G319

Oryza sativa

Os_S76767
1568

71
G319

Zea mays

Zm_S11428226
1770

71
G319

Triticum aestivum

Ta_S281561
1839

71
G319

Lycopersicon

SGN-UNIGENE-50683
1955




esculentum


72
G319

Brassica oleracea

BZ004068

4.00E−37

72
G319

Oryza sativa

AK098865

3.00E−14



( japonica cultivar-



group)

72
G319

Oryza minuta

CB211383

3.00E−14

72
G319

Oryza sativa

AB001888

6.00E−14

72
G319
Oryza sativa ( indica
CA763406

8.00E−14



cultivar-group)

72
G319

Triticum aestivum

AX658811

8.00E−14

72
G319

Sorghum bicolor

CD208331

1.00E−13

72
G319

Zea mays

CD650991

2.00E−13

72
G319

Glycine max

BE058171

4.00E−13

72
G319

Beta vulgaris

BQ589272

7.00E−13

72
G319

Oryza sativa

gi3618320

1.40E−17

72
G319

Oryza sativa

gi23589949

1.90E−11



( japonica cultivar-



group)

72
G319

Malus x domestica

gi4091804

2.20E−10

72
G319

Brassica nigra

gi22854920

5.80E−10

72
G319

Hordeum vulgare

gi21667475

1.90E−09

72
G319

Raphanus sativus

gi3341723

4.90E−09

72
G319

Hordeum vulgare

gi21655156

8.70E−09



subsp. vulgare

72
G319

Brassica napus

gi30984027

1.30E−08

72
G319

Pinus radiata

gi4557093

4.10E−08

72
G319

Ipomoea nil

gi10946337

4.80E−08

74
G324

Oryza sativa

AK068618

1.00E−157



( japonica cultivar-



group)

74
G324

Oryza sativa

AP004141

9.00E−95

74
G324
Oryza sativa ( indica
AAAA01001748

2.00E−94



cultivar-group)

74
G324

Brassica oleracea

BH487952

2.00E−91

74
G324

Brassica napus

CD837146

5.00E−89

74
G324

Lycopersicon

AW979372

5.00E−87




esculentum


74
G324

Phaseolus

CA906556

1.00E−72




coccineus


74
G324

Medicago

CB892723

3.00E−71




truncatula


74
G324

Solanum tuberosum

BG889245

4.00E−70

74
G324

Triticum aestivum

BJ245931

1.00E−69

74
G324

Oryza sativa

gi21742100

2.20E−06



( japonica cultivar-



group)

74
G324

Populus x

gi22795037

0.00055




canescens


74
G324

Oryza sativa

gi15289911

0.0058

74
G324
Rosa hybrid cultivar-
gi15029364

0.084

74
G324

Ipomoea nil

gi11127996

0.087

74
G324
Oryza sativa subsp.
gi7592844

0.088




japonica


74
G324

Lycopersicon

gi4090943

0.17




esculentum


74
G324

Brassica rapa

gi27357054

0.18



subsp. pekinensis

74
G324

Brassica oleracea

gi1418353

0.22

74
G324

Zea diploperennis

gi1076786

0.25

76
G344

Brassica oleracea

BZ490921

2.00E−42

76
G344

Medicago

BE124805

3.00E−40




truncatula


76
G344

Lactuca sativa

BQ865907

2.00E−37

76
G344

Vitis vinifera

BQ800135

5.00E−36

76
G344

Populus tremula x

BU836743

4.00E−33




Populus tremuloides


76
G344

Glycine max

BI470689

9.00E−33

76
G344

Populus

BU876397

1.00E−30



balsamifera subsp.




trichocarpa


76
G344

Lycopersicon

AW030365

1.00E−29




esculentum


76
G344

Zea mays

CC605963

1.00E−29

76
G344

Helianthus annuus

CD855694

2.00E−29

76
G344

Oryza sativa

gi32488102

1.00E−38



( japonica cultivar-



group)

76
G344

Oryza sativa

gi14165317

6.10E−35

76
G344

Nicotiana tabacum

gi12711287

1.70E−27

76
G344

Nicotiana

gi1076609

4.30E−20




plumbaginifolia


76
G344

Atropa belladonna

gi14329812

0.93

76
G344

Glycine max

gi26522778

0.99

76
G344

Ricinus communis

gi112762

1

78
G351

Brassica oleracea

BH552655

2.00E−51

78
G351

Brassica napus

BQ704580

1.00E−47

78
G351

Lotus japonicus

AP004523

6.00E−43

78
G351

Vitis aestivalis

CB288865

6.00E−42

78
G351

Datisca glomerata

AF119050

1.00E−41

78
G351

Petunia x hybrida

PETZFP4

2.00E−39

78
G351

Vitis vinifera

BM437679

2.00E−38

78
G351

Medicago sativa

MSY18788

4.00E−38

78
G351

Glycine max

GMU68763

3.00E−37

78
G351

Lycopersicon

BI421491

3.00E−37




esculentum


78
G351

Petunia x hybrida

gi439493

3.10E−41

78
G351

Oryza sativa

gi28849865

3.00E−35



( japonica cultivar-



group)

78
G351

Datisca glomerata

gi4666360

2.70E−34

78
G351

Brassica rapa

gi2058506

6.70E−32

78
G351

Glycine max

gi1763063

3.70E−30

78
G351

Medicago sativa

gi7228329

3.40E−27

78
G351

Triticum aestivum

gi485814

1.90E−25

78
G351

Oryza sativa

gi12698882

7.70E−25

78
G351

Nicotiana tabacum

gi2981169

9.00E−20

78
G351

Pisum sativum

gi2129892

3.40E−14

79
G355

Glycine max

GLYMA-28NOV01-
747




CLUSTER22443_1

79
G355

Glycine max

GLYMA-28NOV01-
748




CLUSTER22443_2

79
G355

Glycine max

GLYMA-28NOV01-
749




CLUSTER23033_1

79
G355

Glycine max

GLYMA-28NOV01-
750




CLUSTER32665_1

79
G355

Glycine max

GLYMA-28NOV01-
751




CLUSTER32665_2

79
G355

Glycine max

GLYMA-28NOV01-
752




CLUSTER69201_1

79
G355

Oryza sativa

Os_S97951
1569

79
G355

Glycine max

Gma_S5045942
1636

79
G355

Medicago

Mtr_S5425959
1692




truncatula


79
G355

Lycopersicon

SGN-UNIGENE-46187
1956




esculentum


79
G355

Lycopersicon

SGN-UNIGENE-47520
1957




esculentum


79
G355

Lycopersicon

SGN-UNIGENE-
1958




esculentum

SINGLET-6966

80
G355

Brassica oleracea

BH566556

6.00E−59

80
G355

Medicago

AC125368

2.00E−36




truncatula


80
G355

Glycine max

BI967719

4.00E−31

80
G355

Petunia x hybrida

AB006599

6.00E−31

80
G355

Populus tremula x

BU862641

5.00E−29




Populus tremuloides


80
G355

Lycopersicon

BI422808

1.00E−28




esculentum


80
G355

Vitis vinifera

CA818230

4.00E−28

80
G355

Solanum tuberosum

BQ121106

6.00E−27

80
G355

Lotus japonicus

AP006100

1.00E−24

80
G355

Populus

CB239372

2.00E−22



balsamifera subsp.




trichocarpa x





Populus deltoides


80
G355

Petunia x hybrida

gi2346974

6.50E−33

80
G355

Pisum sativum

gi2129892

1.20E−23

80
G355

Oryza sativa

gi29124132

2.70E−20



( japonica cultivar-



group)

80
G355

Oryza sativa

gi18652814

4.00E−17

80
G355

Nicotiana tabacum

gi2981169

1.40E−15

80
G355

Medicago sativa

gi7228329

2.00E−15

80
G355

Datisca glomerata

gi4666360

2.90E−14

80
G355

Triticum aestivum

gi485814

8.60E−14

80
G355

Glycine max

gi1763063

2.10E−13

80
G355

Brassica rapa

gi2058504

1.10E−07

82
G366

Brassica oleracea

BZ046051

8.00E−40

82
G366

Oryza sativa

AP005415

2.00E−10



( japonica cultivar-



group)

82
G366
Oryza sativa ( indica
AAAA01000097

3.00E−10



cultivar-group)

82
G366

Hordeum vulgare

BI960158

4.00E−10

82
G366

Lycopersicon

AW035599

5.00E−10




esculentum


82
G366

Zea mays

CC701812

5.00E−10

82
G366

Medicago

BI308410

8.00E−10




truncatula


82
G366

Vitis vinifera

CB979728

1.00E−09

82
G366

Glycine max

CA784474

1.00E−09

82
G366

Phaseolus

CA902532

1.00E−09




coccineus


82
G366

Oryza sativa

gi29027767

6.20E−12



( japonica cultivar-



group)

82
G366

Sorghum bicolor

gi18390109

5.40E−09

82
G366

Oryza sativa

gi15528588

1.20E−08

82
G366

Petunia x hybrida

gi2346978

2.70E−05

82
G366

Zea ramosa

gi18674684

0.012

82
G366

Triticum aestivum

gi485814

0.09

82
G366

Brassica rapa

gi2058504

0.27

82
G366

Medicago sativa

gi7228329

0.27

82
G366

Glycine max

gi1763063

0.35

82
G366

Pisum sativum

gi2129892

0.44

83
G370

Glycine max

GLYMA-28NOV01-
753




CLUSTER166362_1

83
G370

Glycine max

GLYMA-28NOV01-
754




CLUSTER180202_1

83
G370

Glycine max

GLYMA-28NOV01-
755




CLUSTER726571_1

83
G370

Glycine max

GLYMA-28NOV01-
756




CLUSTER74662_1

83
G370

Glycine max

uC-gmflminsoy032f06b1
757

83
G370

Oryza sativa

ORYSA-22JAN02-
758




CLUSTER173260_2

83
G370

Oryza sativa

ORYSA-22JAN02-
759




CLUSTER200967_1

83
G370

Oryza sativa

OSC100895.C1.p14.fg
760

83
G370

Oryza sativa

OSC23411.C1.p1.fg
761

83
G370

Oryza sativa

OSC2409.C1.p2.fg
762

83
G370

Oryza sativa

OSC25680.C1.p1.fg
763

83
G370

Zea mays

ZEAMA-08NOV01-
764




CLUSTER436044_1

83
G370

Zea mays

ZEAMA-08NOV01-
765




CLUSTER518126_1

83
G370

Oryza sativa

Os_S111189
1570

83
G370

Glycine max

Gma_S5146649
1637

83
G370

Lycopersicon

SGN-UNIGENE-54039
1959




esculentum


83
G370

Lycopersicon

SGN-UNIGENE-54252
1960




esculentum


83
G370

Lycopersicon

SGN-UNIGENE-
1961




esculentum

SINGLET-392715

84
G370

Brassica oleracea

BZ473777

1.00E−98

84
G370

Vitis vinifera

CD714231

5.00E−32

84
G370

Oryza sativa

AK068762

4.00E−31



( japonica cultivar-



group)

84
G370
Oryza sativa ( indica
AAAA01009505

6.00E−30



cultivar-group)

84
G370

Lycopersicon

BG123251

8.00E−30




esculentum


84
G370

Oryza sativa

AC105732

4.00E−26

84
G370

Gossypium

BF272143

3.00E−25




arboreum


84
G370

Hordeum vulgare

BF616974

8.00E−25

84
G370

Sorghum bicolor

BE357942

1.00E−23

84
G370

Zea mays

BH875187

4.00E−23

84
G370

Sorghum bicolor

gi18390109

6.80E−22

84
G370

Oryza sativa

gi15528588

1.10E−09

84
G370

Oryza sativa

gi29027767

1.90E−07



( japonica cultivar-



group)

84
G370

Petunia x hybrida

gi14275902

3.10E−05

84
G370

Glycine max

gi5524682

0.0016

84
G370

Solanum tuberosum

gi13161908

0.0016

84
G370

Medicago sativa

gi7228329

0.0072

84
G370

Datisca glomerata

gi4666360

0.018

84
G370

Zea ramosa

gi18674684

0.05

84
G370

Petroselinum

gi9650824

0.15




crispum


85
G372

Oryza sativa

OSC5500.C1.p4.fg
766

85
G372

Oryza sativa

OSC5501.C1.p10.fg
767

86
G372

Cucurbita pepo

CD726821

1.00E−76

86
G372

Brassica oleracea

BH426533

5.00E−63

86
G372

Medicago

CB892663

6.00E−49




truncatula


86
G372

Glycine max

CA800122

4.00E−48

86
G372

Solanum tuberosum

BG596951

4.00E−45

86
G372

Lactuca sativa

BQ853373

1.00E−38

86
G372

Prunus persica

BU040110

8.00E−38

86
G372

Populus

BI120429

3.00E−36



balsamifera subsp.




trichocarpa


86
G372

Lotus japonicus

AV411356

4.00E−36

86
G372

Helianthus annuus

BU025620

2.00E−35

86
G372

Oryza sativa

gi28564640

3.30E−25



( japonica cultivar-



group)

86
G372

Oryza sativa

gi15289911

6.80E−21

86
G372

Populus x

gi22795037

6.50E−19




canescens


86
G372

Brassica rapa

gi27357054

0.00023



subsp. pekinensis

86
G372

Triticum aestivum

gi32400766

0.00056

86
G372

Cicer arietinum

gi10334499

0.0007

86
G372

Ipomoea nil

gi11127996

0.0015

86
G372

Rosa hybrid cultivar-

gi15029364

0.0015

86
G372

Arabis gemmifera

gi22775495

0.0023

86
G372

Pinus pinaster

gi18129286

0.0041

88
G374

Oryza sativa

AK069434

1.0e−999



( japonica cultivar-



group)

88
G374

Zea mays

AY109632

1.00E−176

88
G374

Brassica napus

CD831794

1.00E−108

88
G374

Lactuca sativa

BQ852311

1.00E−103

88
G374

Helianthus annuus

BQ968130

1.00E−97

88
G374

Medicago

BG646959

2.00E−90




truncatula


88
G374

Solanum tuberosum

BI177742

3.00E−90

88
G374

Lycopersicon

BG128229

9.00E−84




esculentum


88
G374

Hordeum vulgare

BG344938

2.00E−79

88
G374

Beta vulgaris

BQ592292
5
.00E−77

88
G374

Viola cornuta

gi31540598

0.42

88
G374

Oryza sativa

gi29371983

0.83



( japonica cultivar-



group)

90
G380

Oryza sativa

AK071468

1.00E−63



( japonica cultivar-



group)

90
G380

Oryza sativa

AX652813

2.00E−62

90
G380

Medicago

BG581311

2.00E−60




truncatula


90
G380

Solanum tuberosum

BG592404

7.00E−58

90
G380

Lycopersicon

AW625867

1.00E−56




esculentum


90
G380

Lactuca sativa

BQ993132

4.00E−54

90
G380

Gossypium

BQ411449

8.00E−54




arboreum


90
G380

Ipomoea nil

BJ578890

2.00E−53

90
G380

Glycine max

BM143561

8.00E−51

90
G380

Brassica napus

CD827948

4.00E−49

90
G380

Oryza sativa

gi21740878

5.50E−60



( japonica cultivar-



group)

90
G380

Pisum sativum

gi4240031

1.20E−33

90
G380

Glycine max

gi1076498

1.40E−30

90
G380

Oryza sativa

gi8570055

1.10E−25

90
G380

Lotus japonicus

gi1086225

2.30E−25

90
G380

Cicer arietinum

gi10334499

5.00E−16

90
G380
Oryza sativa ( indica
gi29164825

1.40E−10



cultivar-group)

90
G380

Thellungiella

gi20340241

3.30E−08




halophila


90
G380

Triticum aestivum

gi32400766

1.40E−06

90
G380

Zea mays

gi21645888

0.00012

92
G386

Picea abies

AF328842

1.0e−999

92
G386

Oryza sativa

AB101648

1.0e−999



( japonica cultivar-



group)

92
G386

Oryza sativa

AX658854

1.0e−999

92
G386

Zea mays

Zma 13 17898

1.0e−999

92
G386

Malus domestica

AF067961

1.0e−999

92
G386
Phalaenopsis sp.
PSU34743

1.0e−999



SM9108

92
G386
Oryza sativa ( indica
AAAA01007245

1.0e−999



cultivar-group)

92
G386

Gossypium

AF530913

1.00E−179




hirsutum


92
G386

Helianthus annuus

HNNHAHR

1.00E−145

92
G386

Sorghum bicolor

AF466200

1.00E−142

92
G386

Picea abies

gi19070143

1.20E−253

92
G386

Zea mays

gi5531484

3.60E−240

92
G386

Malus x domestica

gi3925363

2.40E−232

92
G386

Oryza sativa

gi32482878

1.00E−218



( japonica cultivar-



group)

92
G386
Phalaenopsis sp.
gi1173622

1.20E−210



SM9108

92
G386
Phalaenopsis sp.
gi2147484

1.20E−210

92
G386

Oryza sativa

gi19072102

3.70E−197

92
G386

Sorghum bicolor

gi18481701

5.00E−177

92
G386

Gossypium

gi22475195

5.20E−159




hirsutum


92
G386

Helianthus annuus

gi1208940

7.30E−121

94
G416

Oryza sativa

AK098855

1.00E−83



( japonica cultivar-



group)

94
G416

Vitis vinifera

CB341898

2.00E−72

94
G416

Zea mays

ZMHOX2AGN

3.00E−64

94
G416

Hedyotis terminalis

CB076461

5.00E−62

94
G416

Petroselinum

PUMPRHPA

5.00E−55




crispum


94
G416

Oryza sativa

CA753304

7.00E−54

94
G416

Hordeum vulgare

BG365916

8.00E−52

94
G416

Sorghum

BF704605

4.00E−48




propinquum


94
G416

Glycine max

BM086637

3.00E−47

94
G416

Lotus japonicus

AP004517

2.00E−44

94
G416

Zea mays

gi1170434

1.30E−78

94
G416

Oryza sativa

gi22830607

1.40E−66



( japonica cultivar-



group)

94
G416

Petroselinum

gi1346791

1.10E−59




crispum


94
G416

Nicotiana tabacum

gi8096269

3.70E−08

94
G416

Lycopersicon

gi9858781

2.70E−05




esculentum


94
G416

Oryza sativa

gi25172773

4.30E−05

94
G416

Cicer arietinum

gi3129939

5.90E−05

94
G416

Medicago sativa

gi1279563

8.10E−05

94
G416

Cucurbita maxima

gi17221648

9.00E−05

94
G416

Raphanus sativus

gi9049359

0.00024

96
G434

Zea mays

ZMA17898

1.00E−126

96
G434

Oryza sativa

AB101648

1.00E−125



( japonica cultivar-



group)

96
G434

Oryza sativa

AX658854

1.00E−124

96
G434

Picea abies

AF328842

1.00E−122

96
G434
Phalaenopsis sp.
PSU34743

1.00E−117



SM9108

96
G434

Malus domestica

AF067961

1.00E−111

96
G434

Gossypium

AF530913

1.00E−103




hirsutum


96
G434
Oryza sativa ( indica
AAAA01007245

1.00E−96



cultivar-group)

96
G434

Helianthus annuus

HNNHAHR

4.00E−82

96
G434

Gossypium

BG444723

7.00E−61




arboreum


96
G434

Oryza sativa

gi31339103

1.90E−123



( japonica cultivar-



group)

96
G434

Zea mays

gi5531484

1.70E−122

96
G434

Oryza sativa

gi19072102

6.70E−115

96
G434
Phalaenopsis sp.
gi1173622

1.80E−114



SM9108

96
G434
Phalaenopsis sp.
gi2147484

1.80E−114

96
G434

Malus x domestica

gi3925363

1.10E−110

96
G434

Picea abies

gi12002853

1.40E−110

96
G434

Gossypium

gi22475195

2.90E−101




hirsutum


96
G434

Sorghum bicolor

gi18481701

6.50E−100

96
G434

Helianthus annuus

gi1208940

5.40E−77

97
G438

Glycine max

GLYMA-28NOV01-
768




CLUSTER24488_2

97
G438

Glycine max

GLYMA-28NOV01-
769




CLUSTER26184_1

97
G438

Glycine max

GLYMA-28NOV01-
770




CLUSTER26184_2

97
G438

Glycine max

GLYMA-28NOV01-
771




CLUSTER26184_4

97
G438

Glycine max

GLYMA-28NOV01-
772




CLUSTER501573_1

97
G438

Glycine max

GLYMA-28NOV01-
773




CLUSTER5380_1

97
G438

Glycine max

GLYMA-28NOV01-
774




CLUSTER5380_2

97
G438

Glycine max

GLYMA-28NOV01-
775




CLUSTER75018_1

97
G438

Glycine max

LIB4164-014-Q1-K1-A6
776

97
G438

Glycine max

LIB4390-042-R1-K2-D5
777

97
G438

Oryza sativa

ORYSA-22JAN02-
778




CLUSTER146698_1

97
G438

Oryza sativa

ORYSA-22JAN02-
779




CLUSTER146698_2

97
G438

Oryza sativa

ORYSA-22JAN02-
780




CLUSTER270598_1

97
G438

Oryza sativa

ORYSA-22JAN02-
781




CLUSTER42938_1

97
G438

Oryza sativa

OSC14111.C1.p2.fg
782

97
G438

Oryza sativa

OSC18647.C1.p19.fg
783

97
G438

Oryza sativa

OSC19346.C1.p14.fg
784

97
G438

Oryza saliva

OSC19540.C1.p1.fg
785

97
G438

Oryza saliva

rsicek_3640.y1.abd
786

97
G438

Oryza sativa

rsicek_9941.y1.abd
787

97
G438

Zea mays

ZEAMA-08NOV01-
788




CLUSTER301_638

97
G438

Zea mays

ZEAMA-08NOV01-
789




CLUSTER301_668

97
G438

Zea mays

ZEAMA-08NOV01-
790




CLUSTER47950_1

97
G438

Zea mays

ZEAMA-08NOV01-
791




CLUSTER495_335

97
G438

Zea mays

ZEAMA-08NOV01-
792




CLUSTER495_404

97
G438

Zea mays

ZEAMA-08NOV01-
793




CLUSTER495_514

97
G438

Zea mays

ZEAMA-08NOV01-
794




CLUSTER745568_1

97
G438

Zea mays

ZEAMA-08NOV01-
795




CLUSTER83671_1

97
G438

Zea mays

uC-zmflteosinte083g09b1
796

97
G438

Zea mays

uC-zmrob73075f05b1
797

97
G438

Oryza sativa

Os_S120953
1571

97
G438

Oryza sativa

Os_S19524
1572

97
G438

Oryza sativa

Os_S23391
1573

97
G438

Oryza sativa

Os_S42908
1574

97
G438

Glycine max

Gma_S4876016
1638

97
G438

Glycine max

Gma_S4999094
1639

97
G438

Glycine max

Gma_S5075763
1640

97
G438

Medicago

Mtr_S5308980
1693




truncatula


97
G438

Medicago

Mtr_S5411708
1694




truncatula


97
G438

Hordeum vulgare

Hv_S197562
1722

97
G438

Hordeum vulgare

Hv_S224721
1723

97
G438

Hordeum vulgare

Hv_S69222
1724

97
G438

Zea mays

Zm_S11477054
1771

97
G438

Zea mays

Zm_S11521524
1772

97
G438

Zea mays

Zm_S11522470
1773

97
G438

Zea mays

Zm_S11523253
1774

97
G438

Zea mays

Zm_S11526859
1775

97
G438

Triticum aestivum

Ta_S130807
1840

97
G438

Triticum aestivum

Ta_S131992
1841

97
G438

Triticum aestivum

Ta_S132089
1842

97
G438

Triticum aestivum

Ta_S25579
1843

97
G438

Triticum aestivum

Ta_S50749
1844

97
G438

Triticum aestivum

Ta_S6425
1845

97
G438

Lycopersicon

Les_S5289520
1927




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-50207
1962




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-50208
1963




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-50279
1964




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-50602
1965




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-50986
1966




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-52670
1967




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-53069
1968




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-54002
1969




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-54245
1970




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-58239
1971




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-58887
1972




esculentum


97
G438

Lycopersicon

SGN-UNIGENE-
1973




esculentum

SINGLET-333165

97
G438

Lycopersicon

SGN-UNIGENE-
1974




esculentum

SINGLET-333872

97
G438

Lycopersicon

SGN-UNIGENE-
1975




esculentum

SINGLET-340997

97
G438

Lycopersicon

SGN-UNIGENE-
1976




esculentum

SINGLET-388971

97
G438

Lycopersicon

SGN-UNIGENE-
1977




esculentum

SINGLET-401049

97
G438

Lycopersicon

SGN-UNIGENE-
1978




esculentum

SINGLET-451497

97
G438

Lycopersicon

SGN-UNIGENE-
1979




esculentum

SINGLET-452871

98
G438

Zinnia elegans

ZEL312053

1.0e−999

98
G438

Oryza sativa

AK102830

1.0e−999



( japonica cultivar-



group)

98
G438

Physcomitrella

AB032182

1.0e−999




patens


98
G438

Zea mays

AY105765

1.0e−999

98
G438

Oryza sativa

AX699680

1.0e−999

98
G438
Oryza sativa ( indica
AAAA01006159

1.00E−165



cultivar-group)

98
G438

Medicago

CB894606

1.00E−128




truncatula


98
G438

Lactuca sativa

BU002601

1.00E−120

98
G438

Poncirus trifoliata

CD574584

1.00E−113

98
G438

Mesernbryanthemum

BE035416

1.00E−106




crystallinum


98
G438

Zinnia elegans

gi18076736

1.0e−999

98
G438

Oryza sativa

gi13384370

1.0e−999

98
G438

Oryza sativa

gi31432701

1.0e−999



( japonica cultivar-



group)

98
G438

Physcomitrella

gi7209912

6.00E−238




patens


98
G438

Ceratopteris

gi3868829

4.20E−35




richardii


98
G438

Sorghum bicolor

gi18481701

5.00E−21

98
G438
Phalaenopsis sp.
gi1173622

1.00E−20



SM9108

98
G438
Phalaenopsis sp.
gi2147484

1.00E−20

98
G438

Picea abies

gi12002853

1.80E−20

98
G438

Zea mays

gi8920427

4.00E−20

100
G446

Oryza sativa

AK103312

1.0e−999



( japonica cultivar-



group)

100
G446

Oryza sativa

AX654320

1.00E−168

100
G446

Mangifera indica

AY255705

1.00E−116

100
G446

Medicago

CB894037

1.00E−114




truncatula


100
G446

Prunus persica

AF467900

1.00E−102

100
G446

Zea mays

AY105215

9.00E−99

100
G446

Beta vulgaris

BQ594500

6.00E−87

100
G446

Populus

AI166599

8.00E−84



balsamifera subsp.




trichocarpa


100
G446
Oryza sativa ( indica
CB631221

8.00E−81



cultivar-group)

100
G446

Pinus pinaster

BX250119

1.00E−80

100
G446

Prunus persica

gi27450533

2.70E−277

100
G446

Oryza sativa

gi19352037

4.80E−203

100
G446

Oryza sativa

gi32489051

5.70E−198



( japonica cultivar-



group)

100
G446

Mangifera indica

gi30027167

5.50E−121

100
G446
Oryza sativa ( indica
gi26251300
2.50E−115



cultivar-group)

100
G446

Mirabilis jalapa

gi23343944

5.10E−20

100
G446

Marchantia

gi25272004

7.40E−11




polymorpha


100
G446

Pisum sativum

gi871511

1.90E−06

100
G446

Lycopersicon

gi1217664

8.30E−06




esculentum


100
G446

Zea mays

gi18697008

9.10E−06

102
G468

Populus tremula x

PTR306827

1.00E−37




Populus tremuloides


102
G468

Glycine max

BU965031

4.00E−33

102
G468

Medicago

BF649039

9.00E−26




truncatula


102
G468

Oryza sativa

AK103865

4.00E−25



( japonica cultivar-



group)

102
G468

Hordeum vulgare

BG301068

2.00E−19

102
G468

Triticum aestivum

BJ228821

5.00E−19

102
G468

Zea mays

BF727992

7.00E−18

102
G468

Helianthus annuus

BU018212

1.00E−17

102
G468

Lycopersicon

BI209073

3.00E−17




esculentum


102
G468

Cycas rumphii

CB089859

3.00E−17

102
G468

Populus tremula x

gi20269055

4.70E−39




Populus tremuloides


102
G468

Oryza sativa

gi8096369

7.90E−26

102
G468

Triticum aestivum

gi32400272

2.70E−20

102
G468
Oryza sativa ( indica
gi30962267

3.40E−20



cultivar-group)

102
G468

Vitis vinifera

gi29465672

6.20E−20

102
G468

Glycine max

gi2388689

8.10E−20

102
G468

Cucumis sativus

gi6136832

1.50E−19

102
G468

Pinus taeda

gi32396293

3.80E−19

102
G468

Mirabilis jalapa

gi23343936

1.00E−18

102
G468

Pisum sativum

gi1352057

1.70E−18

104
G478

Brassica napus

CD839423

1.00E−81

104
G478

Antirrhinum majus

AMA011621

5.00E−75

104
G478

Poncirus trifoliata

CD574568

1.00E−66

104
G478

Brassica oleracea

BH693623

2.00E−58

104
G478

Capsicum annuum

CA516164

7.00E−56

104
G478

Glycine max

AI443033

2.00E−48

104
G478

Medicago

BQ146536

4.00E−46




truncatula


104
G478

Lycopersicon

BI933324

3.00E−45




esculentum


104
G478

Zea mays

ZMA011617

7.00E−44

104
G478

Hedyotis terminalis

CB078220

3.00E−37

104
G478

Antirrhinum majus

gi25458128

7.40E−64

104
G478

Zea mays

gi5931784

2.30E−44

104
G478

Oryza sativa

gi32488855

3.80E−37



( japonica cultivar-



group)

104
G478

Betula pendula

gi30577628

3.00E−28

104
G478

Oryza sativa

gi8468036

4.00E−24

104
G478

Mitochondrion Beta

gi9087308

8.10E−13



vulgaris var.




altissima


104
G478

Lycopersicon

gi7489001

0.00037




esculentum


104
G478

Solanum tuberosum

gi13161908

0.12

104
G478

Glycine max

gi5524682

0.15

104
G478

Triticum aestivum

gi4101568

0.67

105
G485

Oryza sativa

G3394
2135
2.00E−50

105
G485

Oryza sativa

G3395
2137
3.00E−46

105
G485

Oryza sativa

G3396
2139
2.00E−42

105
G485

Oryza sativa

G3397
2141
1.00E−55

105
G485

Oryza sativa

G3398
2143
3.00E−60

105
G485

Oryza sativa

G3429
2145
3.00E−18

105
G485

Zea mays

G3434
2149
1.00E−49

105
G485

Zea mays

G3435
2151
1.00E−57

105
G485

Zea mays

G3436
2153
9.00E−60

105
G485

Zea mays

G3437
2155
3.00E−53

105
G485

Glycine max

G3470
2171
7.00E−46

105
G485

Glycine max

G3471
2173
1.00E−46

105
G485

Glycine max

G3472
2175
3.00E−57

105
G485

Glycine max

G3473
2177
2.00E−53

105
G485

Glycine max

G3474
2179
5.00E−58

105
G485

Glycine max

G3475
2181
5.00E−56

105
G485

Glycine max

G3476
2183
9.00E−57

105
G485

Glycine max

G3477
2185
7.00E−46

105
G485

Glycine max

G3478
2187
3.00E−56

105
G485

Glycine max

GLYMA-28NOV01-
798




CLUSTER24839_1

105
G485

Glycine max

GLYMA-28NOV01-
799




CLUSTER31103_1

105
G485

Glycine max

GLYMA-28NOV01-
800




CLUSTER33504_1

105
G485

Glycine max

GLYMA-28NOV01-
801




CLUSTER33504_3

105
G485

Glycine max

GLYMA-28NOV01-
802




CLUSTER33504_4

105
G485

Glycine max

GLYMA-28NOV01-
803




CLUSTER33504_5

105
G485

Glycine max

GLYMA-28NOV01-
804




CLUSTER33504_6

105
G485

Glycine max

GLYMA-28NOV01-
805




CLUSTER4778_1

105
G485

Glycine max

GLYMA-28NOV01-
806




CLUSTER4778_3

105
G485

Oryza sativa

OSC12630.C1.p5.fg
807

105
G485

Oryza sativa

OSC1404.C1.p3.fg
808

105
0485

Oryza sativa

OSC30077.C1.p6.fg
809

105
G485

Oryza sativa

OSC512.C1.p2.fg
810

105
G485

Oryza sativa

OSC5489.C1.p2.fg
811

105
G485

Oryza sativa

sicef_0681.z1.abd
812

105
G485

Zea mays

LIB3732-044-Q6-K6-C4
813

105
G485

Zea mays

ZEAMA-08NOV01-
814




CLUSTER719_1

105
G485

Zea mays

ZEAMA-08NOV01-
815




CLUSTER719_10

105
0485

Zea mays

ZEAMA-08NOV01-
816




CLUSTER719_2

105
G485

Zea mays

ZEAMA-08NOV01-
817




CLUSTER719_3

105
G485

Zea mays

ZEAMA-08NOV01-
818




CLUSTER719_4

105
G485

Zea mays

ZEAMA-08NOV01-
819




CLUSTER719_5

105
G485

Zea mays

ZEAMA-08NOV01-
820




CLUSTER90408_1

105
G485

Zea mays

ZEAMA-08NOV01-
821




CLUSTER90408_2

105
G485

Glycine max

Gma_S4904793
1641

105
G485

Hordeum vulgare

Hv_S138973
1725

105
G485

Hordeum vulgare

Hv_S17617
1726

105
G485

Zea mays

Zm_S11418173
1776

105
G485

Zea mays

Zm_S11434692
1777

105
G485

Zea mays

Zm_S11509886
1778

105
G485

Triticum aestivum

Ta_S198814
1846

105
G485

Triticum aestivum

Ta_S45374
1847

105
G485

Triticum aestivum

Ta_S50443
1848

105
G485

Triticum aestivum

Ta_S93629
1849

105
G485

Lycopersicon

SGN-UNIGENE-46859
1980




esculentum


105
G485

Lycopersicon

SGN-UNIGENE-47447
1981




esculentum


106
G485

Poncirus trifoliata

CD574709

9.00E−62

106
G485

Solanum tuberosum

BQ505706

4.00E−60

106
G485

Lactuca sativa

BQ996905

2.00E−58

106
G485
Oryza sativa ( indica
AAAA01003638

3.00E−57



cultivar-group)

106
G485

Oryza sativa

AP005193

3.00E−57



(japonica cultivar-



group)

106
G485

Beta vulgaris

BQ592365

9.00E−57

106
G485

Zea mays

CD438068

9.00E−57

106
G485

Physcomitrella

AX288144

3.00E−56




patens


106
G485

Populus

BU880488

1.00E−55



balsamifera subsp.




trichocarpa


106
G485

Glycine max

AX584277

6.00E−55

106
G485

Oryza sativa

gi30409461

4.60E−48



( japonica cultivar-



group)

106
G485

Zea mays

gi115840

9.50E−48

106
G485
Oryza sativa ( indica
gi30349365

1.10E−39



cultivar-group)

106
G485

Oryza sativa

gi15408794

1.60E−38

106
G485

Phaseolus

gi22536010

2.90E−37




coccineus


106
G485

Gossypium

gi28274147

6.30E−35




barbadense


106
G485

Vernonia

gi16902054

2.70E−34




galamensis


106
G485

Glycine max

gi16902050

1.20E−33

106
G485

Argemone mexicana

gi16902056

1.10E−32

106
G485

Triticum aestivum

gi16902058

2.90E−30

108
G521

Brassica oleracea

BH662589

2.00E−78

108
G521

Prunus persica

BU044475

3.00E−71

108
G521

Petunia x hybrida

AF509865

2.00E−67

108
G521

Brassica napus

CD828428

3.00E−67

108
G521

Medicago

AF254124

1.00E−66




truncatula


108
G521

Hordeum vulgare

BJ481205

3.00E−65



subsp. spontaneum

108
G521

Oryza sativa

AK068153

5.00E−65



( japonica cultivar-



group)

108
G521

Hordeum vulgare

BQ469035

1.00E−63

108
G521

Oryza sativa

AX654724

3.00E−63

108
G521
Sorghum
BG241938

7.00E−62




propinquum


108
G521

Petunia x hybrida

gi21105732

7.20E−66

108
G521

Medicago

gi7716952

6.50E−65




truncatula


108
G521

Oryza sativa

gi27452910

1.70E−48



( japonica cultivar-



group)

108
G521

Oryza sativa

gi6730946

4.10E−40

108
G521

Glycine max

gi22597158

1.10E−37

108
G521

Phaseolus vulgaris

gi15148914

4.80E−37

108
G521

Brassica napus

gi31322572

1.00E−36

108
G521

Lycopersicon

gi6175246

4.30E−36




esculentum


108
G521

Solanum tuberosum

gi14485513

5.50E−36

108
G521
Triticum sp.
gi4218535

1.90E−33

110
G549

Brassica oleracea

BOBOFHA

1.0E−999

110
G549

Lycopersicon

AF197934

1.00E−152




esculentum


110
G549

Petunia x hybrida

AF030171

1.00E−150

110
G549

Antirrhinum majus

AMAFLO

1.00E−146



subsp. majus

110
G549

Pisum sativum

AF010190

1.00E−145

110
G549

Eschscholzia

AY188789

1.00E−144



californica subsp.




californica


110
G549

Cucumis sativus

AF059320

1.00E−143

110
G549

Malus x domestica

AB056159

1.00E−139

110
G549

Salix discolor

AY230817

1.00E−138

110
G549

Acacia mangium

AY229890

1.00E−137

110
G549

Brassica oleracea

gi22755

3.40E−201

110
G549

Brassica oleracea

gi487029

3.40E−201



var. botrytis

110
G549

Jonopsidium acaule

gi6003582

1.30E−192

110
G549

Nicotiana tabacum

gi561688

1.30E−144

110
G549

Lycopersicon

gi7658233

2.80E−144




esculentum


110
G549

Petunia x hybrida

gi2625050

8.40E−143

110
G549

Antirrhinum majus

gi100482

1.30E−137

110
G549

Antirrhinum majus

gi166430

1.30E−137



subsp. majus

110
G549

Eschscholzia

gi30313799

1.90E−136



californica subsp.




californica


110
G549

Platanus racemosa

gi8574519

2.20E−135

112
G550

Brassica oleracea

BH930799

1.00E−84

112
G550

Medicago

AC140025

6.00E−82




truncatula


112
G550

Oryza sativa

AP005167

8.00E−74



( japonica cultivar-



group)

112
G550
Oryza sativa ( indica
AAAA01004298

1.00E−73



(cultivar-group)

112
G550

Cucurbita maxima

D45066

7.00E−72

112
G550

Oryza sativa

AX659956

2.00E−67

112
G550

Lycopersicon

BM412713

6.00E−53




esculentum


112
G550

Lactuca sativa

BQ860203

4.00E″52

112
G550

Poncirus trifoliata

CD575555

1.00E−48

112
G550

Glycine max

BM943958

4.00E−45

112
G550

Oryza sativa

gi7242908

2.80E−66

112
G550

Oryza sativa

gi19071625

1.00E−57



( japonica cultivar-



group)

112
G550

Hordeum vulgare

gi21538791

9.10E−43



subsp. vulgare

112
G550

Cucurbita maxima

gi1669341

8.20E−42

112
G550

Dendrobium grex

gi2939325

8.30E−33




Madame Thong-In


112
G550

Hordeum vulgare

gi3777436

2.30E−24

112
G550

Zea mays

gi1346559

1.80E−23

112
G550

Nicotiana tabacum

gi1360078

2.40E−23

112
G550

Pisum sativum

gi6092016

2.40E−23

112
G550

Solanum tuberosum

gi7688355

1.40E−22

114
G571

Oryza sativa

AK103174

1.00E−116



( japonica cultivar-



group)

114
G571

Oryza sativa

AX653996

1.00E−103

114
G571

Phaseolus vulgaris

AF402608

1.00E−100

114
G571

Triticum aestivum

WHTHBP1BC1

1.00E−100

114
G571

Nicotiana tabacum

AF031487

3.00E−98

114
G571

Zea mays

ZMOCSBFB

2.00E−96

114
G571
Oryza sp.
BD261827

1.00E−94

114
G571

Physcomitrella

AX180962

3.00E−93




patens


114
G571

Vicia faba

VFACREBL

7.00E−89

114
G571

Glycine max

BQ611848

7.00E−88

114
G571

Phaseolus vulgaris

gi15148924

1.50E−95

114
G571

Nicotiana tabacum

gi6288682

8.50E−95

114
G571

Zea mays

gi297018

4.80E−92

114
G571

Oryza sativa

gi13872972

7.90E−92

114
G571

Triticum aestivum

gi1076782

1.00E−91

114
G571

Oryza sativa

gi33146487

4.30E−91



( japonica cultivar-



group)

114
G571

Glycine max

gi7488179

9.10E−75

114
G571

Vicia faba

gi100099

1.90E−74

114
G571
Nicotiana sp.
gi19680

1.10E−71

114
G571

Solanum tuberosum

gi7489280

1.20E−70

116
G581

Gerbera hybrida

GHY7709

3.00E−73

116
G581

Lotus uliginosus

AF503362

1.00E−57

116
G581

Brassica oleracea

BZ019501

3.00E−57

116
G581

Gossypium

AF336279

5.00E−54




hirsutum


116
G581

Antirrhinum majus

AMADEL

5.00E−53

116
G581

Oryza sativa

AB021080

7.00E−52

116
G581

Perilla frutescens

AB024050

2.00E−51

116
G581

Petunia x hybrida

AF020545

3.00E−51

116
G581

Populus

BU875274

2.00E−49



balsamifera subsp.




trichocarpa


116
G581

Medicago

BI308638

6.00E−48




truncatula


116
G581

Lotus uliginosus

gi20467247

6.80E−92

116
G581

Gossypium

gi13346182

2.40E−81




hirsutum


116
G581

Perilla frutescens

gi4519199

6.40E−79

116
G581

Antirrhinum majus

gi166428

8.30E−75

116
G581

Zea mays

gi100897

3.50E−69

116
G581

Oryza sativa

gi1086540

1.20E−63

116
G581

Gerbera hybrida

gi3650292

5.20E−63

116
G581

Lotus japonicus

gi20467249

6.70E−61

116
G581

Oryza sativa

gi32488805

8.80E−57



( japonica cultivar-



group)

116
G581

Petunia x hybrida

gi3127045

8.00E−51

118
G600

Brassica oleracea

BZ035190

2.00E−41

118
G600

Helianthus annuus

BQ914741

2.00E−25

118
G600

Medicago

AW688852

3.00E−24




truncatula


118
G600

Glycine max

AW703971

3.00E−22

118
G600

Populus tremula x

BU830207

1.00E−18




Populus tremuloides


118
G600

Vitis vinifera

CB913112

2.00E−17

118
G600

Gossypium

BQ413633

3.00E−15




arboreum


118
G600

Lactuca sativa

BQ859538

3.00E−11

118
G600

Hedyotis

CB086932

4.00E−11




centranthoides


118
G600

Zea mays

CA830375

2.00E−09

118
G600

Oryza sativa

gi31432245

1.30E−20



( japonica cultivar-



group)

118
G600

Oryza sativa

gi11034640

1.50E−06

118
G600

Lycopersicon

gi9858781

4.50E−06




esculentum


118
G600

Medicago sativa

gi3334756

5.50E−06

118
G600

Nicotiana

gi3850821

2.50E−05




plumbaginifolia


118
G600

Cucurbita maxima

gi17221648

7.00E−05

118
G600

Zea mays

gi11340599

9.80E−05

118
G600

Chlamydomonas

gi28207761

0.002




reinhardtii


118
G600

Nicotiana tabacum

gi8096269

0.0023

118
G600

Pisum sativum

gi7440062

0.027

119
G624

Glycine max

Gma_S4875227
1642

120
G624

Zea mays

AY103971

1.00E−56

120
G624

Oryza sativa

AK101356

7.00E−48



( japonica cultivar-



group)

120
G624
Oryza sativa ( indica
CB620477

8.00E−42



cultivar-group)

120
G624

Solanum tuberosum

BQ517157

2.00E−41

120
G624

Sorghum bicolor

BE600697

8.00E−41

120
G624

Hordeum vulgare

BG367957

2.00E−39

120
G624

Lactuca sativa

BQ865528

3.00E−34

120
G624

Lycopersicon

AW737389

6.00E−34




esculentum


120
G624

Zinnia elegans

AU293844

1.00E−31

120
G624

Glycine max

BF070672

3.00E−25

120
G624

Oryza sativa

gi23617202

4.50E−95



( japonica cultivar-



group)

120
G624

Oryza sativa

gi391885

3.90E−13

120
G624

Daucus carota

gi5578746

4.30E−13

120
G624

Mesembryanthemum

gi3219155

7.30E−13




crystrallinum


120
G624

Fagopyrum

gi32469224

1.90E−12




esculentum


120
G624

Solanum tuberosum

gi27528486

4.20E−12

120
G624

Zea mays

gi100922

5.60E−12

120
G624

Hordeum vulgare

gi1730475

6.30E−12



subsp. vulgare

120
G624

Eragrostis tef

gi17906977

6.30E−12

120
G624

Craterostigma

gi2288899

7.10E−12




plantagineum


121
G627

Glycine max

GLYMA-28NOV01-
822




CLUSTER65192_1

121
G627

Glycine max

GLYMA-28NOV01-
823




CLUSTER65192_2

121
G627

Glycine max

GLYMA-28NOV01-
824




CLUSTER495_1

121
G627

Oryza sativa

Os_S65371
1575

121
G627

Medicago

Mtr_S5455444
1695




truncatula


121
G627

Hordeum vulgare

Hv_S12327
1727

121
G627

Triticum aestivum

Ta_S329524
1850

121
G627

Lycopersicon

SGN-UNIGENE-58075
1982



esculentum


121
G627

Populus tremuloides

AF377868

3.00E−60

122
G627

Eucalyptus globulus

AF086642

1.00E−59



subsp. globulus

122
G627

Petunia x hybrida

AF335239

1.00E−58

122
G627

Pimpinella

AF082531

1.00E−58




brachycarpa


122
G627

Populus tremula x

BU896825

3.00E−58




Populus tremuloides


122
G627

Cardamine flexuosa

AY257542

2.00E−57

122
G627

Nicotiana tabacum

NTTOB

3.00E−57

122
G627

Sinapis alba

SAU25696

4.00E−57

122
G627

Brassica rapa

AY257541

7.00E−57



subsp. pekinensis

122
G627

Oryza sativa

AF141965

3.00E−55

122
G627

Populus tremuloides

gi31295609

1.00E−59

122
G627

Eucalyptus globulus

gi4322475

2.70E−59



subsp. globulus

122
G627

Pimpinella

gi3493647

8.20E−58




brachycarpa


122
G627

Petunia x hybrida

gi13384056

1.00E−57

122
G627

Sinapis alba

gi1049022

2.50E−56

122
G627

Nicotiana tabacum

gi1076646

2.50E−56

122
G627

Cardamine flexuosa

gi30171309

2.50E−56

122
G627

Brassica rapa

gi30171307

3.20E−56



subsp. pekinensis

122
G627

Elaeis guineensis

gi6635740

2.00E−54

122
G627

Oryza sativa

gi5295990

5.30E−54

124
G646

Brassica oleracea

BH437513

3.00E−71

124
G646

Oryza sativa

AB028129

2.00−53

124
G646

Brassica napus

CD813699

1.00E−52

124
G646
Oryza sativa ( indica
AAAA01002346

2.00E−51



cultivar-group)

124
G646

Oryza sativa

AK060659

1.00E−50



( japonica cultivar-



group)

124
G646

Populus tremula BU821375


8.00E−49

124
G646

Vitis vinifera

CB910264

1.00E−48

124
G646

Nicotiana tabacum

NTA9594

2.00E−46

124
G646

Glycine max

BE611146

1.00E−44

124
G646

Solanum tuberosum

BG592323

9.00E−43

124
G646

Oryza sativa

gi32482863

4.90E−51


( japonica cultivar-



group)

124
G646

Oryza sativa

gi4996640

4.90E−51

124
G646

Hordeum vulgare

gi20372847

9.50E−50



subsp. vulgare

124
G646

Nicotiana tabacum

gi1360084

2.70E−42

124
G646

Pisum sativum

gi6092016

2.30E−35

124
G646

Triticum aestivum

gi3790264

4.70E−33

124
G646

Solanum tuberosum

gi7688355

3.20E−32

124
G646

Hordeum vulgare

gi3777436

8.50E−32

124
G646

Zea mays

gi2393775

2.00E−31

124
G646

Cucurbita maxima

gi1669341

2.70E−27

125
G646

Glycine max

GLYMA-28NOV01-
825




CLUSTER252329_1

125
G651

Glycine max

GLYMA-28NOV01-
826




CLUSTER28671_1

125
G651

Glycine max

GLYMA-28NOV01-
827




CLUSTER28671_2

125
G651

Glycine max

GLYMA-28NOV01-
828




CLUSTER38144_1

125
G651

Oryza sativa

ORYSA-22JAN02-
829




CLUSTER12764_1

125
G651

Oryza sativa

OSC100181.C1.p3.fg
830

125
G651

Oryza sativa

OSC100807.C1.p14.fg
831

125
G651

Oryza sativa

OSC12064.C1.p26.fg
832

125
G651

Oryza sativa

OSC4083.C1.p1.fg
833

125
G651

Zea mays

LIB4171-012-R1-K1-B10
834

125
G651

Oryza sativa

Os_S113261
1576

125
G651

Zea mays

Zm_S11367031
1779

125
G651

Zea mays

Zm_S11431995
1780

125
G651

Zea mays

Zm_S11525554
1781

125
G651

Lycopersicon

SGN-UNIGENE-50411
1983




esculentum


125
G651

Lycopersicon

SGN-UNIGENE-
1984




esculentum

SINGLET-34777

125
G651

Lycopersicon

SGN-UNIGENE-
1985




esculentum

SINGLET-7514

126
G651

Petunia x hybrida

AB035133

4.00E−44

126
G651

Brassica oleracea

BZ474306

3.00E−40

126
G651

Oryza sativa

AP005072

5.00E−31



( japonica cultivar-



group)

126
G651
Oryza sativa ( indica
AAAA01000406

5.00E−31



cultivar-group)

126
G651

Lactuca sativa

BU015249

9.00E−31

126
G651

Vitis vinifera

CA808162

3.00E−29

126
G651

Oryza sativa

AC037426

3.00E−29

126
G651

Glycine max

BU548087

1.00E−25

126
G651

Zea mays

BZ372896

1.00E−25

126
G651

Solanum tuberosum

BQ508073

3.00E−25

126
G651

Petunia x hybrida

gi2346986

5.50E−36

126
G651

Oryza sativa

gi21740840

1.60E−32



( japonica cultivar-



group)

126
G651

Medicago sativa

gi7228329

1.70E−16

126
G651

Glycine max

gi1763063

1.20E−15

126
G651

Datisca glomerata

gi4666360

3.20E−15

126
G651

Oryza sativa

gi15623826

8.80E−13

126
G651

Brassica rapa

gi2058504

1.20E−12

126
G651

Nicotiana tabacum

gi2981169

6.40E−12

126
G651

Triticum aestivum

gi485814

2.30E−11

126
G651

Pisum sativum

gi2129892

3.40E−10

126
G651

Glycine max

BG362937.1
835

127
G652

Glycine max

GLYMA-28NOV01-
836




CLUSTER3357_10

127
G652

Glycine max

GLYMA-28NOV01-
837




CLUSTER3357_14

127
G652

Glycine max

GLYMA-28NOV01-
838




CLUSTER3357_15

127
G652

Glycine max

GLYMA-28NOV01-
839




CLUSTER3357_8

127
G652

Glycine max

GLYMA-28NOV01-
840




CLUSTER3357_9

127
G652

Glycine max

GLYMA-28NOV01-
841




CLUSTER380953_1

127
G652

Glycine max

GLYMA-28NOV01-
842




CLUSTER4027_1

127
G652

Oryza sativa

ORYSA-22JAN02-
843




CLUSTER51249_2

127
G652

Oryza sativa

ORYSA-22JAN02-
844




CLUSTER7171_1

127
G652

Oryza sativa

OSC100158.C1.p6.fg
845

127
G652

Oryza sativa

OSC101188.C1.p2.fg
846

127
G652

Oryza sativa

OSC101621.C1.p8.fg
847

127
G652

Oryza sativa

OSC19412.C1.p4.fg
848

127
G652

Oryza sativa

OSC20041.C1.p3.fg
849

127
G652

Oryza sativa

OSC21434.C1.p27.fg
850

127
G652

Oryza sativa

OSC24160.C1.p7.fg
851

127
G652

Oryza sativa

OSC24791.C1.p2.fg
852

127
G652

Oryza sativa

uC-osflcyp173f05b1
853

127
G652

Zea mays

ZEAMA-08NOV01-
854




CLUSTER447_18

127
G652

Zea mays

ZEAMA-08NOV01-
855




CLUSTER447_20

127
G652

Zea mays

ZEAMA-08NOV01-
856




CLUSTER447_21

127
G652

Zea mays

ZEAMA-08NOV01-
857




CLUSTER447_23

127
G652

Zea mays

ZEAMA-08NOV01-
858




CLUSTER55_18

127
G652

Zea mays

ZEAMA-08NOV01-
859




CLUSTER55_22

127
G652

Zea mays

ZEAMA-08NOV01-
860




CLUSTER55_44

127
G652

Oryza sativa

Os_S118507
1577

127
G652

Oryza sativa

Os_S42588
1578

127
G652

Oryza sativa

Os_S46064
1579

127
G652

Glycine max

Gma_S4871214
1643

127
G652

Glycine max

Gma_S4965905
1644

127
G652

Glycine max

Gma_S5135351
1645

127
G652

Hordeum vulgare

Hv_S107672
1728

127
G652

Hordeum vulgare

Hv_S142991
1729

127
G652

Hordeum vulgare

Hv_S147464
1730

127
G652

Zea mays

Zm_S11487070
1782

127
G652

Triticum aestivum

Ta_S109795
1851

127
G652

Triticum aestivum

Ta_S2509
1852

127
G652

Triticum aestivum

Ta_S45732
1853

127
G652

Triticum aestivum

Ta_S60357
1854

127
G652

Triticum aestivum

Ta_S75244
1855

127
G652

Lycopersicon

Les_S5162139
1928




esculentum


127
G652

Lycopersicon

SGN-UNIGENE-56979
1986




esculentum


127
G652

Lycopersicon

SGN-UNIGENE-
1987




esculentum

SINGLET-39394

128
G652

Brassica oleracea

BH926980

7.00E−90

128
G652

Nicotiana sylvestris

NSGRP2MR

2.00E−71

128
G652

Zea mays

AI812203

1.00E−64

128
G652

Solanum tuberosum

BM408211

5.00E−64

128
G652

Oryza sativa

AP003879

9.00E−64

128
G652

Oryza sativa

AK101577

9.00E−64



( japonica cultivar-



group)

128
G652

Oryza sativa (
indica

AAAA01000576

1.00E−62



cultivar-group)

128
G652

Triticum aestivum

AB066265

2.00E−62

128
G652

Aegilops speltoides

BQ840577

3.00E−62

128
G652

Pinus pinaster

BX249354

9.00E−61

128
G652

Nicotiana sylvestris

gi121631

1.10E−67

128
G652

Oryza sativa

gi29467522

6.00E−62



( japonica cultivar-



group)

128
G652

Triticum aestivum

gi21322752

2.00E−61

128
G652

Chlamydomonas

gi30527347

2.40E−26




reinhardtii


128
G652

Phaseolus vulgaris

gi121628

6.20E−26

128
G652

Nicotiana tabacum

gi395147

8.70E−25

128
G652

Brassica napus

gi17821

1.80E−23

128
G652

Petunia x hybrida

gi121627

2.20E−23

128
G652
Petunia sp.
gi225181

2.20E−23

128
G652

Oryza sativa

gi15528745

2.50E−22

130
G707

Picea abies

AF328842

1.0e−999

130
G707

Oryza sativa

AB101648

1.0e−999



( japonica cultivar-



group)

130
G707

Oryza sativa

AX658854

1.0e−999

130
G707

Zea mays

ZMA250985

1.0e−999

130
G707

Malus domestica

AF067961

1.0e−999

130
G707
Phalaenopsis sp.
PSU34743

1.0e−999



SM9108

130
G707
Oryza sativa ( indica
AAAA01007245

1.0e−999



cultivar-group)

130
G707

Gossypium

AF530913

1.00E−172




hirsutum


130
G707

Sorghum bicolor

AF466200

1.00E−141

130
G707

Helianthus annuus

HNNHAHR

1.00E−138

130
G707

Picea abies

gi19070143

3.20E−237

130
G707

Oryza sativa

gi31339101

1.00E−231



( japonica cultivar-



group)

130
G707

Malus x domestica

gi3925363

4.10E−230

130
G707
Phalaenopsis sp.
gi1173622

2.40E−200



SM9108

130
G707
Phalaenopsis sp.
gi2147484

2.40E−200

130
G707

Oryza sativa

gi19072102

2.90E−197

130
G707

Zea mays

gi8920421

1.10E−195

130
G707

Sorghum bicolor

gi18481701

5.90E−190

130
G707

Gossypium

gi22475195

4.80E−168




hirsutum


130
G707

Helianthus annuus

gi1208940

3.70E−127

132
G728

Brassica oleracea

BH471284

4.00E−41

132
G728

Oryza sativa

AK105625

1.00E−40



( japonica cultivar-



group)

132
G728

Lycopersicon

AW032021

3.00E−30




esculentum


132
G728

Prunus persica

BU040714
4.00E−28

132
G728

Prunus armeniaca

CB820349
1.00E−27

132
G728

Populus tremula x

BU886442

2.00E−27




Populus tremuloides


132
G728

Triticum aestivum

BU100819

2.00E−27

132
G728

Populus

CB239440

2.00E−26



balsamifera subsp.




trichocarpa x





Populus deltoides


132
G728

Vitis vinifera

CB979887

2.00E−25

132
G728

Triticum

BQ803556

6.00E−25




monococcum


132
G728

Oryza sativa

gi33146555

2.40E−4



( japonica cultivar-



group)

132
G728

Oryza sativa

gi11034542

5.70E−35

132
G728

Nicotiana tabacum

gi4519671

1.00E−11

132
G728

Chlamydomonas

gi5916207

2.80E−11




reinhardtii


132
G728

Zea mays

gi15667625

5.40E−11

132
G728
Mesembryanthemum
gi6942190

1.30E−10




crystallinum


132
G728

Solanum

gi32470629

1.70E−09




bulbocastanum


132
G728

Oryza glaberrima

gi31338862

2.70E−08

132
G728

Oryza sativa (
indica

gi31338860

4.50E−08



cultivar-group)

132
G728

Glycine max

gi23821873

0.064

134
G730

Brassica oleracea

BH976893

2.00E−73

134
G730

Medicago

GA922031

1.00E−41




truncatula


134
G730

Vitis vinifera

CB005926

4.00E−36

134
G730

Lactuca sativa

BU004983

5.00E−34

134
G730

Glycine max

B0363182

2.00E−31

134
G730

Triticum aestivum

CA498340

4.00E−30

134
G730

Solanum tuberosum

BQ115344

1.00E−28

134
G730

Brassica napus

CD817870

3.00E−28

134
G730

Populus tremula x

AI163121

6.00E−27




Populus tremuloides


134
G730

Oryza sativa

AK108408

1.00E−26



( japonica cultivar-



group)

134
G730

Oryza sativa

gi29467563

6.40E−41



( japonica cultivar-



group)

134
G730

Nicotiana tabacum

gi4519671

1.80E−15

134
G730

Mesembryanthemum

gi6942190

5.20E−15




crystallinum


134
G730

Solanum

gi32470629

8.00E−13




bulbocastanum


134
G730

Chlamydomonas

gi5916207

1.90E−12




reinhardtii



134
G730

Oryza sativa

gi11034542

1.70E−08

134
G730

Oryza glaberrima

gi31338862

6.40E−08

134
G730
Oryza sativa ( indica
gi31338860

4.00E−07



cultivar-group)

134
G730

Zea mays

gi15667625

1.00E−05

134
G730

Theophrasta

gi12004107

0.41




americana


136
G738

Medicago

BF636532

5.00E−37




truncatula


136
G738

Brassica oleracea

BH568823

7.00E−36

136
G738

Glycine max

BE555532

1.00E−35

136
G738

Nicotiana tabacum

NTA9594

2.00E−34

136
G738

Lycopersicon

AI894846

7.00E−33




esculentum


136
G738

Capsicum annuum

CA847307

8.00E−32

136
G738

Vitis vinifera

CB910264

1.00E−31

136
G738

Solanum tuberosum

BM113542

6.00E−31

136
G738

Zea mays

CC698952

6.00E−31

136
G738

Oryza sativa

OSJN00155

4.00E−30

136
G738

Nicotiana tabacum

gi3341468

3.90E−35

136
G738

Hordeum vulgare

gi20372847

6.00E−33



subsp. vulgare

136
G738

Oryza sativa

gi32482863

2.50E−31



( japonica cultivar-



group)

136
G738

Oryza sativa

gi4996640

2.50E−31

136
G738

Pisum sativum

gi6092016

8.30E−30

136
G738

Solanum tuberosum

gi7688355

2.10E−27

136
G738

Zea mays

gi2393775

2.70E−27

136
G738

Triticum aestivum

gi3790264

7.10E−27

136
G738

Hordeum vulgare

gi3777436

4.10E−24

136
G738

Cucurbita maxima

gi1669341

2.00E−22

138
G744

Oryza sativa

AK070302

2.00E−63



( japonica cultivar-



group)

138
G744

Lactuca sativa

BQ856329

5.00E−59

138
G744

Vitis vinifera

CA817874

2.00E−57

138
G744
Oryza sativa ( indica
CA767518

5.00E−55



cultivar-group)

138
G744

Brassica oleracea

BH430310

6.00E−54

138
G744

Triticum aestivum

BJ258750

1.00E−51

138
G744

Zea mays

AW257837

1.00E−50

138
G744

Hordeum vulgare

BF628902

2.00E−47



subsp. vulgare

138
G744

Pinus pinaster

BX252059

1.00E−44

138
G744

Brassica napus

AI352966

6.00E−42

138
G744

Oryza sativa

gi18855037

2.40E−35

138
G744

Oryza sativa

gi31433346

8.10E−35



( japonica cultivar-



group)

138
G744

Zea mays

gi18092342

1.20E−13

138
G744

Nicotiana tabacum

gi12003386

5.40E−13

138
G744

Cucumis melo

gi17016985

1.40E−12

138
G744

Hordeum vulgare

gi20152976

8.20E−11



subsp. vulgare

138
G744

Hordeum vulgare

gi2894379

2.00E−09

138
G744

Medicago sativa

gi23451086

5.60E−08

138
G744

Glycine max

gi1076498

1.00E−06

138
G744

Pisum sativum

gi4240031

1.40E−06

140
G752

Brassica oleracea

BZ499460

1.00E−70

140
G752

Medicago

CA920567

2.00E−58




truncatula


140
G752

Poncirus trifoliata

CD573735

7.00E−55

140
G752

Zea mays

CD436549

1.00E−54

140
G752

Oryza sativa

AK066069

6.00E−54



( japonica cultivar-



group)

140
G752

Oryza sativa

AX653661

6.00E−54

140
G752

Lycopersicon

BI923342

2.00E−52




esculentum


140
G752

Hordeum vulgare

AV946923

1.00E−49



subsp. spontaneum

140
G752
Oryza sativa ( indica
CB627816

2.00E−49



cultivar-group)

140
G752

Glycine max

AI438025

5.00E−48

140
G752

Oryza sativa

gi21740878

4.30E−29



( japonica cultivar-



group)

140
G752

Pisum sativum

gi4240031

1.50E−27

140
G752

Glycine max

gi1076498

3.30E−27

140
G752

Lotus japonicus

gi1086225

8.40E−26

140
G752

Oryza sativa

gi8570055

8.70E−23

140
G752

Cicer arietinum

gi10334499

1.10E−13

140
G752
Oryza sativa ( indica
gi29164825

2.60E−07



cultivar-group)

140
G752

Triticum aestivum

gi32400766

1.00E−05

140
G752

Zea mays

gi18092342

1.60E−05

140
G752

Tulipa gesneriana

gi23386073

0.00011

141
G807

Oyrza sativa

G3491
2201
1E−114

141
G807

Glycine max

G3494
2203
1E−116

141
G807

Glycine max

G3495
2205
1E−117

141
G807

Glycine max

G3512
2207
1E−118

141
G807

Glycine max

GLYMA-28NOV01-
861




CLUSTER700_1

141
G807

Glycine max

GLYMA-28NOV01-
862




CLUSTER700_2

141
G807

Glycine max

GLYMA-28NOV01-
863




CLUSTER700_3

141
G807

Oryza sativa

ORYSA-22JAN02-
864




CLUSTER7494_1

141
G807

Oryza sativa

OSC19953.C1.p7.fg
865

141
G807

Zea mays

ZEAMA-08NOV01-
866




CLUSTER20750_1

141
G807

Zea mays

ZEAMA-08NOV01-
867




CLUSTER8272_1

141
G807
Oryza sativa
Os _S33091
1580

141
G807

Hordeum vulgare

Hv_S99692
1731

141
G807

Zea mays

Zm_S11426353
1783

141
G807

Lycopersicon

SGN-UNIGENE-57622
1988




esculentum


141
G807

Lycopersicon

SGN-UNIGENE-
1989




esculentum

SINGLET-334447

142
G807

Lycopersicon

LPHSF8

1.00E−112




peruvianum


142
G807

Oryza sativa

AK106118

1.00E−111



( japonica cultivar-



group)

142
G807

Brassica napus

CD814788

6.00E−96

142
G807

Medicago

AC087771

4.00E−82




truncatula


142
G807

Solanum tuberosum

BG890899

1.00E−80

142
G807
Oryza sativa ( indica
AAAA01005302

6.00E−80



cultivar-group)

142
G807

Oryza sativa

AC120506

5.00E−79

142
G807

Lycopersicon

LEHSF8

4.00E−78




esculentum


142
G807

Glycine max

AW569256

2.00E−76

142
G807
Populus tremula x
BU834690

1.00E−73




Populus tremuloides


142
G807

Oryza sativa

gi29126355

4.80E−101



( japonica cultivar-



group)

142
G807

Lycopersicon

gi100264

1.30E−100




peruvianum


142
G807

Lycopersicon

gi100225

1.60E−100




esculentum


142
G807

Nicotiana tabacum

gi5821138

2.90E−56

142
G807

Phaseolus

gi16118447

1.10E−52




acutifolius


142
G807

Medicago sativa

gi20162459

2.80E−50

142
G807

Glycine max

gi662924

1.90E−49

142
G807

Zea mays

gi2130134

1.40E−48

142
G807

Oryza sativa

gi14209551

1.70E−48

142
G807

Helianthus annuus

gi25052685

1.80E−46

144
G811

Solanum tuberosum

BG889138

8.00E−70

144
G811

Lycopersicon

AW738534

1.00E−64




esculentum


144
G811

Lactuca sativa

BQ854304

3.00E−64

144
G811

Glycine max

BE347442

2.00E−52

144
G811

Euphorbia esula

AW874988

2.00E−50

144
G811

Capsicum annuum

CA514873

4.00E−50

144
G811

Vitis vinifera

CB920522

4.00E−48

144
G811

Oryza sativa

AK106488

1.00E−47



( japonica cultivar-



group)

144
G811

Triticum aestivum

CD909725

7.00E−47

i44
G811

Oryza sativa

AX652911

2.00E−46

i44
G811

Oryza sativa

gi15624016

1.60E−47

144
G811

Oryza sativa

gi20161000

1.60E−47



( japonica cultivar-



group)

144
G811

Lycopersicon

gi100264

3.60E−39




peruvianum


144
G811

Lycopersicon

gi100225

4.70E−39




esculentum


144
G811

Glycine max

gi2129831

5.40E−39

144
G811

Nicotiana tabacum

gi5821138

4.30E−36

144
G811

Medicago sativa

gi20162459

8.80E−36

144
G811

Zea mays

gi2130134

3.90E−35

144
G811

Phaseolus

gi16118447

1.70E−32




acutifolius


144
G811

Helianthus annuus

gi25052685

2.80E−32

145
G839

Glycine max

GLYMA-28NOV01-
868




CLUSTER16158_1

145
G839

Glycine max

GLYMA-28NOV01-
869




CLUSTER66623_1

145
G839

Glycine max

LIB4164-057-R1-N1-A5
870

145
G839

Oryza sativa

ORYSA-22JAN02-
871




CLUSTER117082_1

145
G839

Oryza sativa

OSC2170.C1.p5.fg
872

145
G839

Oryza sativa

OSC28351.C1.p1.fg
873

145
G839

Oryza sativa

rsicek_9913.y1.abd
874

145
G839

Zea mays

ZEAMA-08NOV01-
875




CLUSTER1121_63

145
G839

Zea mays

ZEAMA-08NOV01-
876




CLUSTER7249_1

145
G839

Glycine max

Gma_S4891477
1646

145
G839

Glycine max

Gma_S6669519
1647

145
G839

Zea mays

Zma_S11525703
1784

145
G839

Triticum aestivum

Ta_S276434
1856

145
G839

Lycopersicon

SGN-UNIGENE-53309
1990




esculentum


145
G839

Lycopersicon

SGN-UNIGENE-58458
1991




esculentum


145
G839

Lycopersicon

SGN-UNIGENE-
1992




esculentum

SINGLET-453084

146
G839

Nicotiana tabacum

BD260600

1.00E−176

146
G839

Helianthus annuus

BD260596

1.00E−171

146
G839

Zea mays

AX041006

1.00E−158

146
G839

Triticum aestivum

BD263897

1.00E−154

146
G839

Oryza sativa

AK067198

1.00E−149



( japonica cultivar-



group)

146
G839

Oryza sativa

AX653720

1.00E−137

146
G839

Beta vulgaris

BD260595

1.00E−110

146
G839

Lycopersicon

BD260557

1.00E−110




esculentum


146
G839

Brassica napus

AF527176

7.00E−97

146
G839

Brassica oleracea

BH483537

3.00E−90

146
G839

Zea mays

gi11340603

2.00E−153

146
G839

Triticum aestivum

gi18616497

5.20E−145

146
G839

Oryza sativa

gi22535593

2.30E−144



( japonica cultivar-



group)

146
G839

Oryza sativa

gi18616493

2.80E−131

146
G839

Nicotiana tabacum

gi21552981

2.00E−111

146
G839

Brassica napus

gi22003730

1.80E−94

146
G839

Chlamydomonas

gi30025990

0.14




reinhardtii


146
G839

Nicotiana alata

gi26418416

0.22

146
G839

Pennisetum ciliare

gi549986

0.64

146
G839

Gossypium

gi29837373

0.9




herbaceum


148
G846

Oryza sativa

AK100130

1.0e−999



( japonica cultivar-



group)

148
G846

Oryza sativa

AX652964

1.0e−999

148
G846

Medicago

CB893601

1.00E−102




truncatula


148
G846
Oryza sativa ( indica
AAAA01003006

5.00E−98



cultivar-group)

148
G846

Triticum aestivum

BQ842184

1.00E−92

148
G846

Helianthus annuus

BU028886

5.00E−89

148
G846

Lactuca sativa

BQ869409

8.00E−87

148
G846

Brassica oleracea

BZ039976

6.00E−83

148
G846

Lycopersicon

BE433450

1.00E−74




esculentum


148
G846
Populus tremula x
BU894371

1.00E−70




Populus tremuloides


148
G846

Oryza sativa

gi15289872

3.50E−215

148
G846

Oryza sativa

gi32489674

3.50E−176



( japonica cultivar-



group)

148
G846

Zea mays

gi18463957

1.10E−33

148
G846

Triticum

gi23193487

1.80E−28




monococcum


148
G846

Hordeum vulgare

gi23193479

2.90E−28



subsp. vulgare

148
G846

Hordeum vulgare

gi23193481

3.70E−28

148
G846
Rosa hybrid cultivar-
gi15029364

0.0017

148
G846

Populus x

gi22795037

0.013




canescens


148
G846

Glycine max

gi25172766

0.018

148
G846

Arabis gemmifera

gi22775495

0.022

150
G852

Oryza sativa

AK060833

1.00E−155



( japonica cultivar-



group)

150
G852

Brassica oleracea

BZ426102

1.00E−121

150
G852
Oryza sativa ( indica
CB628520

1.00E−115



cultivar-group)

150
G852

Oryza sativa

AP003747

1.00E−112

150
G852

Medicago

AC137079

1.00E−107




truncatula


150
G852

Zea mays

AY109543

1.00E−105

150
G852

Glycine max

BQ629578

4.00E−89

150
G852

Triticum aestivum

BQ744552

2.00E−88

150
G852

Vitis vinifera

CB980495

2.00E−86

150
G852

Solanum tuberosum

BQ510154

5.00E−86

150
G852

Oryza sativa

gi20161431

5.00E−149



( japonica cultivar-



group)

150
G852

Lilium longiflorum

gi32813435

1.20E−55

150
G852

Oryza sativa

gi14719333

2.20E−54

150
G852

Vitis vinifera

gi20334379

5.20E−53

150
G852

Zea mays

gi10178637

1.10E−52

150
G852

Pisum sativum

gi13365610

7.20E−50

150
G852

Lycopersicon

gi31322802

1.50E−49




esculentum


150
G852

Brassica rapa

gi28143934

5.80E−48



subsp. pekinensis

150
G852

Gossypium

gi29122893

2.00E−47




hirsutum


150
G852

Carlquistia muirii

gi20257447

2.50E−47

152
G905

Brassica oleracea

BZ481426

7.00E−97

152
G905

Medicago

AC136503

2.00E−52




truncatula


152
G905

Cucumis melo

AF499727

3.00E−48

152
G905

Citrus sinensis

CB290516

9.00E−41

152
G905

Poncirus trifoliata

GD576402

4.00E−39

152
G905

Populus

BU878367

2.00E−38



balsamifera subsp.




trichocarpa


152
G905

Capsicum annuum

BM063816

2.00E−37

152
G905

Gossypium

AI730749

5.00E−37




hirsutum


152
G905
Oryza sativa ( indica
AAAA01018235

2.00E−36



cultivar-group)

152
G905

Oryza sativa

AP004164

2.00E−36



( japonica cultivar-



group)

152
G905

Cucumis melo

gi28558782

6.50E−49

152
G905

Oryza sativa

gi21740711

4.70E−30

152
G905

Oryza sativa

gi24756877

4.30E−29



( japonica cultivar-



group)

152
G905

Medicago sativa

gi23451086

6.90E−19

152
G905

Nicotiana tabacum

gi12003386

1.20E−18

152
G905

Hordeum vulgare

gi20152976

5.80E−15



subsp. vulgare

152
G905

Zea mays

gi21645888

1.00E−14

152
G905

Hordeum vulgare

gi2894379

2.00E−12

152
G905

Solanum tuberosum

gi24745601

8.70E−11

152
G905

Thellungiella

gi20340241

1.10E−09




halophila


153
G916

Glycine max

BE021411.1
877

153
G916

Glycine max

BG652320.1
878

153
G916

Glycine max

GLYMA-28NOV01-
879




CLUSTER191657_1

153
G916

Glycine max

GLYMA-28NOV01-
880




CLUSTER3354_1

153
G916

Glycine max

GLYMA-28NOV01-
881




CLUSTER3354_2

153
G916

Glycine max

GLYMA-28NOV01-
882




CLUSTER3354_3

153
G916

Glycine max

GLYMA-28NOV01-
883




CLUSTER3354_4

153
G916

Glycine max

GLYMA-28NOV01-
884




CLUSTER47179_1

153
G916

Oryza sativa

OSC101573.C1.p8.fg
885

153
G916

Oryza sativa

OSC1429.C1.p2.fg
886

153
G916

Oryza sativa

OSC18885.C1.p17.fg
887

153
G916

Oryza sativa

rsicee_8920.y1.abd
888

153
G916

Glycine max

Gma_S4878547
1648

153
G916

Glycine max

Gma_S6668474
1649

153
G916

Hordeum vulgare

Hv_S119532
1732

153
G916

Zea mays

Zm_S11388469
1785

153
G916

Lycopersicon

SGN-UNIGENE-47034
1993




esculentum


153
G916

Lycopersicon

SGN-UNIGENE-47543
1994




esculentum


153
G916

Lycopersicon

SGN-UNIGENE-52279
1995




esculentum


153
G916

Lycopersicon

SGN-UNIGENE-
1996




esculentum

SINGLET-18500

153
G916

Lycopersicon

SGN-UNIGENE-
1997




esculentum

SINGLET-1941

153
G916

Lycopersicon

SGN-UNIGENE-
1998




esculentum

SINGLET-20683

154
G916

Oryza sativa

AX653053

7.00E−97

154
G916

Citrus sinensis

BQ625082

2.00E−81

154
G916

Medicago

CB893379

3.00E−80




truncatula


154
G916

Glycine max

BU926713

5.00E−77

154
G916
Oryza sativa ( indica
AAAA01004053

4.00E−76



cultivar-group)

154
G916

Oryza sativa

AC120986

1.00E−75



( japonica cultivar-



group)

154
G916

Lycopersicon

AI895084

5.00E−71




esculentum


154
G916

Prunus persica

BU047549

1.00E−69

154
G916

Glycine clandestina

BG838724

7.00E−66

154
G916

Brassica oleracea

BH710263

1.00E−58

154
G916

Oryza sativa

gi11320830

1.50E−98

154
G916

Nicotiana tabacum

gi30013667

3.90E−42

154
G916

Oryza sativa

gi20160973

1.50E−33



( japonica cultivar-



group)

154
G916

Avena fatua

gi1159879

2.80E−30

154
G916

Petroselinum

gi11493822

5.30E−27




crispum


154
G916

Pimpinella

gi3420906

2.30E−23




brachycarpa


154
G916

Capsella rubella

gi32454266

1.00E−22

154
G916

Lycopersicon

gi13620227

2.00E−21




esculentum


154
G916

Ipomoea batatas

gi1076685

4.50E−21

154
G916

Avena sativa

gi4894965

9.60E−21

155
G926

Glycine max

GLYMA-28NOV01-
889




CLUSTER3291_14

155
G926

Glycine max

GLYMA-28NOV01-
890




CLUSTER3291_6

155
G926

Oryza sativa

ORYSA-22JAN02-
891




CLUSTER2283_3

155
G926

Oryza sativa

ORYSA-22JAN02-
892




CLUSTER56491_1

155
G926

Oryza sativa

uC-osrocyp029g09a1
893

155
G926

Zea mays

ZEAMA-08NOV01-
894




CLUSTER15892_1

155
G926

Triticum aestivum

Ta_S91478
1857

155
G926

Lycopersicon

SGN-UNIGENE-52928
1999




esculentum


155
G926

Lycopersicon

SGN-UNIGENE-57779
2000




esculentum


155
G926

Lycopersicon

SGN-UNIGENE-
2001




esculentum

SINGLET-15562

156
G926

Prunus dulcis

BU573158

1.00E−56

156
G926

Medicago

BI310587

3.00E−55




truncatula


156
G926

Citrus sinensis

BQ624240

1.00E−47

156
G926

Brassica oleracea

BH443554

4.00E−44

156
G926

Helianthus

CF082573

5.00E−40




paradoxus


156
G926

Brassica napus

BNU33885

2.00E−39

156
G926

Lycopersicon

BF113081

1.00E−37




esculentum


156
G926

Solanum tuberosum

BG886494

3.00E−36

156
G926

Glycine max

AW472517

4.00E−36

156
G926

Gossypium

BQ407583

7.00E−36




arboreum


156
G926

Brassica napus

gi1173616

1.20E−40

156
G926

Oryza sativa

gi27552556

2.60E−36



( japonica cultivar-



group)

156
G926

Oryza sativa

gi2826786

1.30E−27

156
G926

Vitis riparia

gi7141243

7.10E−27

156
G926

Nicotiana tabacum

gi4731314

5.00E−19

156
G926

Vicia faba

gi2104675

0.0075

156
G926

Hordeum vulgare

gi21667471

0.71

156
G926

Phaseolus vulgaris

gi13775107

0.74

156
G926

Solanum tuberosum

gi1096930

0.76

156
G926

Zea mays

gi1839593

0.84

158
G957

Brassica oleracea

BH998192

1.00E−86

158
G957

Populus

BU879250

6.00E−82



balsamifera subsp.




trichocarpa


158
G957

Oryza sativa

AK109860

2.00E−74



( japonica cultivar-



group)

158
G957

Hordeum vulgare

BE060921

1.00E−68

158
G957

Medicago

BF645745

3.00E−68




truncatula


158
G957

Lycopersicon

BF098091

3.00E−67




esculentum


158
G957

Oryza sativa

AB028186

4.00E−66

158
G957
Oryza sativa ( indica
AAAA01001925

1.00E−63



cultivar-group)

158
G957

Populus

CA826386

2.00E−63



balsamifera subsp.




trichocarpa x





Populus deltoides


158
G957

Triticum aestivum

BQ483881

1.00E−60

158
G957

Oryza sativa

gi11875152

5.40E−78

158
G957

Oryza sativa

gi28190666

5.40E−78



( japonica cultivar-



group)

158
G957

Phaseolus vulgaris

gi15148914

8.80E−45

158
G957

Glycine max

gi22597158

7.90E−44

158
G957

Petunia x hybrida

gi1279640

4.40E−43

158
G957

Brassica napus

gi31322568

6.40E−42

158
G957

Lycopersicon

gi6175246

7.40E−41




esculentum


158
G957

Solanum tuberosum

gi14485513

3.50E−40

158
G957

Triticum sp.

gi4218537

8.40E−40

158
G957

Triticum

gi6732160

8.40E−40




monococcum


159
G961

Glycine max

GLYMA-28NOV01-
895




CLUSTER264630_1

159
G961

Glycine max

jC-
896




gmXLIB3563P021ad08d2

159
G961

Oryza sativa

ORYSA-22JAN02-
897




CLUSTER6410_1

159
G961

Oryza sativa

OSC21400.C1.p3.fg
898

159
G961

Zea mays

LIB4743-075-R1-K1-
899




G11

159
G961

Zea mays

ZEAMA-08NOV01-
900




CLUSTER13382_1

159
G961

Zea mays

ZEAMA-08NOV01-
901




CLUSTER13382_2

159
G961

Glycine max

Gma_S5137324
1650

159
G961

Lycopersicon

SGN-UNIGENE-
2002




esculentum

SINGLET-366637

160
G961

Oryza sativa

AK109860

1.00E−88



( japonica cultivar-



group)

160
G961

Brassica oleracea

BZ522709

6.00E−84

160
G961

Populus

BU879250

3.00E−81



balsamifera subsp.




trichocarpa


160
G961

Hordeum vulgare

BE060921

3.00E−72

160
G961

Lycopersicon

BF098091

3.00E−70




esculentum


160
G961

Glycine max

BU547985

4.00E−69

160
G961

Medicago

BF645892

3.00E−67




truncatula


160
G961

Oryza sativa

AP002542

3.00E−66

160
G961
Oryza sativa ( indica
AAAA01001925

3.00E−66



cultivar-group)

160
G961

Triticum aestivum

CD878476

2.00E−63

160
G961

Oryza sativa

gi11875152

5.00E−83

160
G961

Oryza sativa

gi28190666

5.00E−83



( japonica cultivar-



group)

160
G961

Glycine max

gi22597158

1.10E−46

160
G961

Phaseolus vulgaris

gi15148914

1.30E−45

160
G961

Petunia x hybrida

gi1279640

2.00E−45

160
G961
Triticum sp.
gi4218537

3.00E−44

160
G961

Triticum

gi6732160

3.00E−44




monococcum


160
G961

Brassica napus

gi31322582

7.90E−44

160
G961

Zea mays

gi32527660

7.10E−43

160
G961

Lycopersicon

gi6175246

2.80E−41




esculentum


161
G975

Glycine max

AW705973.1
902

161
G975

Glycine max

BE610471.1
903

161
G975

Glycine max

GLYMA-28NOV01-
904




CLUSTER232634_1

161
G975

Glycine max

GLYMA-28NOV01-
905




CLUSTER8245_1

161
G975

Glycine max

GLYMA-28NOV01-
906




CLUSTER84865_1

161
G975

Oryza sativa

ORYSA-22JAN02-
907




CLUSTER256875_1

161
G975

Oryza sativa

OSC33871.C1.p4.fg
908

161
G975

Oryza sativa

rsicek_16488.y1.abd
909

161
G975

Zea mays

BG874224.1
910

161
G975

Zea mays

ZEAMA-08NOV01-
911




CLUSTER277338_1

161
G975

Hordeum vulgare

Hv_S31912
1733

161
G975

Lycopersicon

SGN-UNIGENE-52816
2003




esculentum


161
G975

Lycopersicon

SGN-UNIGENE-
2004




esculentum

SINGLET-14957

161
G975

Lycopersicon

SGN-UNIGENE-
2005




esculentum

SINGLET-330976

161
G975

Lycopersicon

SGN-UNIGENE-
2006




esculentum

SINGLET-335836

162
G975

Brassica napus

D838135

2.00E−91

162
G975

Brassica oleracea

BH477624

2.00E−69

162
G975

Triticum aestivum

CA486875

4.00E−64

162
G975

Oryza sativa

AK061163

3.00E−62



( japonica cultivar-



group)

162
G975

Oryza sativa

AX699685

2.00E−61

162
G975

Rosa chinensis

BI978981

3.00E−60

162
G975

Amborella

CD484088

3.00E−59




trichopoda


162
G975

Hordeum vulgare

BU978490

2.00E−58



subsp. vulgare

162
G975

Vitis aestivalis

CB289393

7.00E−58

162
G975

Lycopersicon

BG642554

1.00E−56




esculentum


162
G975

Oryza sativa

gi32479658

2.20E−30



( japonica cultivar-



group)

162
G975

Lycopersicon

gi18650662

2.20E−25




esculentum


162
G975

Lupinmus polyphyllus

ge131754

2.60E−22

162
G975

Nicotiana tabacum

gi3065895

1.10E−19

162
G975

Atriplex hortensis

gi8571476

1.10E−19

162
G975

Zea mays

gi21908036

1.00E−18

162
G975

Stylosanthes hamata

gi4099914

1.30E−18

162
G975

Hordeum vulgare

gi27960757

1.70E−18

162
G975

Oryza sativa

gi1056106

2.00E−18

162
G975

Nicotiana sylvestris

gi8809573

1.20E−17

163
G1011

Glycine max

GLYMA-28NOV01-
912




CLUSTER36089_1

163
G1011

Glycine max

GLYMA-28NOV01-
913




CLUSTER36089_2

163
G1011

Glycine max

GLYMA-28NOV01-
914




CLUSTER36089_3

163
G1011

Glycine max

GLYMA-28NOV01-
915




CLUSTER36089_4

163
G1011

Glycine max

GLYMA-28NOV01-
916




CLUSTER36089_6

163
G1011

Glycine max

GLYMA-28NOV01-
917




CLUSTER475715_2

163
G1011

Oryza sative

ORYSA-22JAN02-
918




CLUSTER475_3

163
G1011

Oryza sative

OSC101782.C1.p2.fg
919

163
G1011

Zea mays

ZEAMA-08NOV01-
920




CLUSTER48_1

163
G1011

Zea mays

ZEAMA-08NOV01-
921




CLUSTER48_2

163
G1011

Zea mays

ZEAMA-08NOV01-
922




CLUSTER48_4

163
G1011

Zea mays

ZEAMA-08NOV01-
923




CLUSTER48_5

163
G1011

Zea mays

ZEAMA-08NOV01-
924




CLUSTER8143_1

163
G1011

Oryza sativa

Os_S60918
1581

163
G1011

Glycine max

Gma_S5094568
1651

163
G1011

Medicago

Mtr_S5357829
1696




truncatula


163
G1011

Zea mays

Zm_S11418746
1786

163
G1011

Zea mays

Zm_S11527819
1787

163
G1011

Triticum aestivum

Ta_S203038
1858

163
G1011

Triticum aestivum

Ta_S304256
1859

163
G1011

Triticum aestivum

Ta_S424724
1860

163
G1011

Lycopersicon

Les_S5295933
1929




esculentum


163
G1011

Lycopersicon

SGN-UNIGENE-50586
2007




esculentum


163
G1011

Lycopersicon

SGN-UNIGENE-52410
2008




esculentum


163
G1011

Lycopersicon

SGN-UNIGENE-
2009




esculentum

SINGLET-366830

163
G1011

Lycopersicon

SGN-UNIGENE-2010




esculentum

SINGLET-394847

164
G1011

Petunia x hybrida

AF335240

1.00E−58

164
G1011

Sinapis alba

SAU25696

5.00%−58

164
G1011

Brassica rapa

AY257541

1.00E−57



subsp. pekinensis

164
G1011

Lycopersicon

AI486684

1.00E−57




esculentum


164
G1011

Cardamine flexuosa

AY257542

2.00E−57

164
G1011

Vitis vinifera

CA808988

3.00E−57

164
G1011

Populus tremuloides

AF377868

9.00E−57

164
G1011

Pimpinella

AF082531

8.00E−56




brachycarpa


164
G1011

Eucalyptus grandis

AY263808

2.00E−55

164
G1011

Draba nemorosa

AY257543

8.00E−55



var. hebecarpa

164
G1011

Petunia x hybrida

gi13384058

3.90E−58

164
G1011

Sinapis alba

gi1049022

4.50E−57

164
G1011

Brassica rapa

gi30171307

5.70E−57



subsp. pekinensis

164
G1011

Cardamine flexuosa

gi30171309

1.90E−56

164
G1011

Populus tremuloides

gi31295609

1.40E−55

164
G1011

Pimpinella

gi3493647

7.60E−55




brachycarpa


164
G1011

Nicotiana tabacum

gi1076646

1.60E−54

164
G1011

Eucalyptus grandis

gi30575600

1.60E−54

164
G1011

Draba nemorosa

gi30171311

1.10E−53

164
G1011

Eucalyptus

gi30983946

1.10E−53




occidentalis


165
G1013

Oryza sativa

OSC102289.C1.p7.fg
925

166
G1013

Oryza sativa

AK110625

6.00E−36



( japonica cultivar-



group)

166
G1013

Lotus japonicus

AV419754

5.00E−21

166
G1013

Glycine max

BU082967

3.00E−17

166
G1013

Oryza sativa

BI799118

4.00E−17

166
G1013

Amborella

CD483414

6.00E−17




trichopoda


166
G1013

Zea mays

BM269291

1.00E−16

166
G1013

Triticum aestivum

BE445081

1.00E−16

166
G1013

Beta vulgaris

BQ490186

1.00E−16

166
G1013

Prunus persica

BU044499

1.00E−16

166
G1013

Hordeum vulgare

CB868568

1.00E−16

166
G1013

Oryza sativa

gi15289994

2.40E−39

166
G1013

Oryza sativa

gi20160927

2.40E−39



( japonica cultivar-



group)

166
G1013

Nicotiana tabacum

gi14530683

1.30E−18

166
G1013

Solanum tuberosum

gi24745606

1.30E−18

166
G1013

Cucumis sativus

gi7484759

2.90E−17

166
G1013

Capsella rubella

gi13620168

3.00E−17

166
G1013

Ipomoea batatas

gi1076685

7.30E−17

166
G1013

Petroselinum

gi1432058

3.70E−16




crispum


166
G1013

Retama raetam

gi18158619

5.30E−16

166
G1013

Lycopersicon

gi13620227

5.50E−16




esculentum


168
G1017

Oryza sativa

AK100322

1.0e−999



( japonica cultivar-



group)

168
G1017

Oryza sativa

AB071299

1.0e−999

168
G1017
Oryza sativa ( indica
AAAA01008877

1.00E−170



cultivar-group)

168
G1017

Lotus japonicus

AP004505

1.00E−139

168
G1017

Physcomitrella

AX288142

1.00E−130




patens


168
G1017

Zea mays

CC657791

1.00E−104

168
G1017

Brassica oleracea

BH736206

1.00E−101

168
G1017

Medicago

BG646821

3.00E−98




truncatula


168
G1017

Vitis vinifera

CB979491

2.00E−87

168
G1017

Triticum aestivum

BJ258796

5.00E−70

168
G1017

Oryza sativa

gi19352051

1.40E−194

168
G1017

Oryza sativa

gi13384374

7.90E−179



( japonica cultivar-



group)

168
G1017

Mangifera indica

gi30027167

5.50E−73

168
G1017
Oryza sativa ( indica
gi26251300

3.10E−72



cultivar-group)

168
G1017

Prunus persica

gi27450533

5.00E−70

168
G1017

Marchantia

gi25272004

2.60E−11




polymorpha


168
G1017

Mirabilis jalapa

gi23343944

0.00011

168
G1017

Zea mays

gi18697008

0.00015

168
G1017

Pisum sativum

gi1352057

0.01

168
G1017
Populus tremula x
gi20269063

0.1




Populus tremuloides


170
G1033

Brassica napus

CD822227

6.00E−72

170
G1033

Medicago

BI264539

8.00E−60




truncatula


170
G1033

Glycine max

BI967499

3.00E−59

170
G1033

Hedyotis

CB087944

7.00E−58




centranthoides


170
G1033

Phaseolus

CA902244

7.00E−56




coccineus


170
G1033

Pisum sativum

CD860485

5.00E−54

170
G1033

Helianthus annuus

CD856695

5.00E−54

170
G1033

Capsicum annuum

BM063385

9.00E−53

170
G1033

Vitis vinifera

CA818147

2.00E−52

170
G1033

Lycopersicon

AI776807

2.00E−52




esculentum


170
G1033

Ipomoea nil

gi1052956

6.10E−37

170
G1033

Nicotiana tabacum

gi2196548

7.80E−37

170
G1033

Solanum tuberosum

gi2894109

7.00E−36

170
G1033

Pisum sativum

gi436424

1.10E−35

170
G1033

Glycine max

gi123379

3.90E−35

170
G1033
Oryza sativa ( indica
gi23345287

4.50E−34



cultivar-group)

170
G1033

Oryza sativa

gi3885888

4.50E−34

170
G1033

Zea mays

gi2196672

1.50E−33

170
G1033

Vicia faba

gi541981

2.50E−33

170
G1033

Triticum aestivum

gi100791

3.10E−33

171
G1037

Glycine max

GLYMA-28NOV01-
926




CLUSTER33215
_1

171
G1037 Glycine max
GLYMA-28NOV01-
927




CLUSTER35769_1

171
G1037 Glycine max
GLYMA-28NOV01-
928




CLUSTER35769_2

171
G1037 Glycine max
GLYMA-28NOV01-
929




CLUSTER78853_1

171
G1037 Oryza sativa
LIB4309-004-Q1-K1-F10
930

171
G1037 Oryza sativa
ORYSA-22JAN02-
931




CLUSTER7899_1

171
G1037 Oryza sativa
OSC101221.C1.p7.fg
932

171
G1037 Oryza sativa
OSC101630.C1.p9.fg
933

171
G1037 Oryza sativa
OSC101746.C1.p5.fg
934

171
G1037 Oryza sativa
OSC101754.C1.p6.fg
935

171
G1037 Oryza sativa
OSC15521.C1.p1.fg
936

171
G1037 Oryza sativa
OSC22451.C1.p8.fg
937

171
G1037 Oryza sativa
rsicek_16168.y1.abd
938

171
G1037 Zea mays
ZEAMA-08NOV01-
939




CLUSTER1091_1

171
G1037 Zea mays
ZEAMA-08NOV01-
940




CLUSTER586757_1

171
G1037 Zea mays
ZEAMA-08NOV01-
941




CLUSTER724_633

171
G1037 Glycine max
Gma_S4903453
1652

171
G1037

Zea mays

Zm_S11418484
1788

171
G1037

Zea mays

Zm_S11440157
1789

171
G1037

Triticum aestivum

Ta_S103284
1861

171
G1037

Triticum aestivum

Ta_S349182
1862

171
G1037

Lycopersicon

SGN-UNIGENE-
2011




esculentum

SINGLET-68090

172
G1037

Oryza sativa

AK101165

1.00E−100



( japonica cultivar-



group)

172
G1037

Zea mays

AB062095

4.00E−94

172
G1037
Oryza sativa ( indica
CB630542

6.00E−90



cultivar-group)

172
G1037

Brassica oleracea

BH007675

6.00E−87

172
G1037

Solanum tuberosum

BM407041

6.00E−77

172
G1037

Medicago

BG450692

7.00E−71




truncatula


172
G1037

Lactuca sativa

BQ858556

4.00E−62

172
G1037

Stevia rebaudiana

BG523436

1.00E−60

172
G1037

Vitis vinifera

CD800109

2.00E−58

172
G1037

Oryza sativa

AP004552

1.00E−56

172
G1037

Zea mays

gi14189890

1.80E−92

172
G1037

Oryza sativa

gi24308616

3.80E−76



( japonica cultivar-



group)

172
G1037

Oryza glabberrima

gi31338862

5.40E−45

172
G1037
Oryza sativa ( indica
gi31338860

1.80E−44



cultivar-group)

172
G1037

Oryza sativa

gi15289981

1.30E−20

172
G1037

Dianthus

gi13173408

100E−10




caryophyllus


172
G1037

Nicotiana tabacum

gi4519671

6.80E−09

172
G1037

Mesembryanthemum

gi6942190

2.20E−07




crystallinum


172
G1037

Chlamydomonas

gi5916207

3.30E−07



reinhardtii


172
G1037

Solanum

gi32470629

2.60E−06




bulbocastanum


172
G1082

Oryza sativa

AK100643

1.00E−144



( japonica cultivar-



group)

174
G1082

Brassica oleraccea

BH666792

1.00E−90

174
G1082

Solanum tuberosum

BI179090

7.00E−77

174
G1082

Glycine max

CA785487

3.00E−75

174
G1082

Helianthus annuus

BU026443

4.00E−67

174
G1082

Populus

BU883184

2.00E−63



balsamifera subsp.




trichocarpa


174
G1082

Hordeum vulgare

CA030236

3.00E−60



subsp. vulgare

174
G1082

Triticum aestivum

BJ288629

2.00E−55

174
G1082
Oryza sativa ( indica
AAAA01001043

5.00E−54



cultivar-group)

174
G1082

Lactuca sativa

BQ860209

6.00E−54

174
G1082

Oryza sativa

gi32483081

4.10E−79



( japonica cultivar-



group)

174
G1082

Gossypium

gi22858664

2.10E−46




hirsutum


174
G1082

Oryza sativa

gi13124871

1.10E−43

174
G1082

Marsilea

gi22550110

1.10E−15




quadrifolia


174
G1082

Catharanthus

Gi1486263

2.70E−07




roseus


174
G1082

Lupinus

gi28912428

2.50E−05




angustifolius


174
G1082

Glycine max

gi347455

3.10E−05

174
G1082

Vigna unguiculata

gi1076556

3.80E−05

174
G1082

Lycopersicon

gi100210

6.10E−05




esculentum


174
G1082

Nicotiana tabacum

gi296617

7.70E−05

176
G1100

Brassica oleracea

BZ515453

8.00E−61

176
G1100

Medicago

CB892846

1.00E−41




truncatula


176
G1100

Cucumis melo

AF499727

2.00E−41

176
G1100

Solanum tuberosum

BG590574

3.00E−31

176
G1100

Citrus sinensis

B290516

1.00E−30

176
G1100

Zea mays

CC6505834

3.00E−30

176
G1100

Poncirus trifoliata

CD576402

3.00E−30

176
G1100

Gossypium

AI730749

2.00E−29




hirsutum


176
G1100

Oryza sativa

AP005399

1.00E−28



( japonica cultivar-



group)

176
G1100

Glycine max

BQ094213

1.00E−28

176
G1100

Cucumis melo

gi28558782

6.50E−45

176
G1100

Oryza sativa

gi24756877

2.10E−27



( japonica cultivar-



group)

176
G1100

Oryza sativa

gi21740711

3.80E−25

176
G1100

Medicago sativa

gi23451086

7.00E−20

176
G1100

Nicotiana tabacum

gi12003386

3.50E−15

176
G1100

Zea mays

gi21645888

1.80E−12

176
G1100

Hordeum vulgare

gi20152976

2.70E−12



subsp. vulgare

176
G1100

Hordeum vulgare

gi2894379

4.90E−12

176
G1100

Solanum tuberosum

gi24745601

4.80E−10

176
G1100

Cicer arietinum

gi4651204

2.90E−08

178
G1108

Oryza sativa

AK066424

1.00E−113



( japonica cultivar-



group)

178
G1108

Zea mays

BG837939

1.00E−91

178
G1108

Brassica oleracea

BZ486328

1.00E−89

178
G1108

Lactuca sativa

BQ852089

3.00E−80

178
G1108

Triticum aestivum

BJ319065

2.00E−78

178
G1108
Oryza sativa ( indica
CB634885

5.00E−78



cultivar-group)

178
G1108

Lycopersicon

BI921710

1.00E−75




esculentum


178
G1108

Oryza sativa

AX699700

1.00E−73

178
G1108

Hordeum vulgare

AL505242

8.00E−71



subsp. vulgare

178
G1108

Solanum tuberosum

BQ512426

6.00E−69

178
G1108

Oryza sativa

gi15289774

6.00E−78



( japonica cultivar-



group)

178
G1108

Phacelia

gi5002214

1.40E−28




tanacetifolia


178
G1108

Medicago sativa

gi23451086

5.10E−12

178
G1108

Oryza sativa

gi14164470

1.10E−11

178
G1108

Cicer arietinum

gi4651204

2.60E−10

178
G1108

Nicotiana tabacum

gi2003386

1.40E−09

178
G1108

Thellungiella

gi20340241

1.50E−09




halophila


178
G1108

Hordeum vulgare

gi2894379

2.80E−09

178
G1108

Cucumis melo

gi17016985

2.30E−08

178
G1108

Hordeum vulgare

gi20152976

3.10E−08



subsp. vulgare

180
G1113

Brassica napus

AI352907

7.00E−49

180
G1113

Brassica rapa

BG543052

1.00E−26



subsp. pekinensis

180
G1113

Ipomoea nil

BJ574282

2.00E−25

180
G1113

Populus tremula

BU893088

3.00E−25

180
G1113

Lactuca sativa

BQ849490

3.00E−25

180
G1113

Populus tremula x

BU885427

4.00E−25




Populus tremuloides


180
G1113

Gossypium

AI729600

8.00E−24




hirsutum


180
G1113

Populus

CA825344

8.00E−24



balsamifera subsp.




trichocarpa x





Populus deltoides


180
G1113

Lycopersicon

AW034559

1.00E−23




esculentum


180
G1113

Capsicum annuum

CA847343

3.00E−23

180
G1113

Oryza sativa

gi32488512

6.10E−19



( japonica cultivar-



group)

180
G1113

Oryza sative

gi14164467

2.40E−12

180
G1113

Hordeum vulgare

gi20152976

4.90E−12



subsp. vulgare

180
G1113

Thellungiella

gi20340241

1.30E−11




halophila


180
G1113

Medicago sativa

gi23451086

2.60E−11

180
G1113

Cucumis melo

gi28558782

1.80E−10

180
G1113

Glycine max

gi22597166

6.50E−10

180
G1113

Nicotiana tabacum

gi12003386

2.90E−09

180
G1113

Zea mays

gi18092342

1.30E−08

180
G1113

Pisum sativum

gi4240031

2.40E−08

181
G1128

Glycine max

GLYMA-28NOV01-
942




CLUSTER83680_3

181
G1128

Oryza sativa

OSC795.C1.p2.fg
943

181
G1128

Glycine max

Gma_S4918384
1653

181
G1128

Triticum aestivum

Ta_S190134
1863

181
G1128

Brassica oleracea

BH586480

1.00E−82

181
G1128

Glycine max

BU926769

7.00E−60

181
G1128

Oryza sativa

AK068379

3.00E−58



( japonica cultivar-



group)

181
G1128

Gossypium

BG441060

5.00E−53




arboreum


182
G1128

Populus tremula x

BU814921

1.00E−50




Populus tremuloides


181
G1128

Solanum tuberosum

BQ508721

4.00E−48

181
G1128

Hordeum vulgare

BQ765321

3.00E−47

182
G1128

Populus

BI139442

6.00E−47



balsamifera subsp.




trichocarpa


182
G1128

Medicago

BG589060

1.00E−46




truncatula


182
G1128

Zinnia elegans

AU289368

4.00E−45

182
G1128

Oryza sativa

gi12643044

5.20E−51



( japonica cultivar-



group)

182
G1128

Pisum sativum

gi2213534

2.50E−45

182
G1128

Antirrhinum majus

gi4165183

3.40E−38

182
G1128

Vitis vinifera

gi30421132

0.0005

182
G1128

Zea mays

gi9837562

0.0019

182
G1128

Oryza sativa

gi21740825

0.0026

182
G1128

Spinacia oleracea

gi6492266

0.0033

182
G1128

Nicotiana tabacum

gi6492262

0.068

182
G1128

Volvox carteri f.

gi4324621

0.078




nagariensis


182
G1128

Lycopersicon

gi15144504

0.11




esculentum


184
G1136

Phaseolus vulgaris

PVU18348

1.00E−156

184
G1136

Gossypium

CA994239

1.00E−138




raimondii


184
G1136

Oryza sativa

AE017122

1.00E−100



( japonica cultivar-



group)

184
G1136

Oryza sativa

AC060755

1.00E−100

184
G1136

Brassica oleracea

BZ466433

7.00E−86

184
G1136

Zea mays

AF061107

1.00E−80

184
G1136

Lycopersicon

AF011557

2.00E−73




esculentum


184
G1136

Solanum tuberosum

BI434651

2.00E−72

184
G1136
Oryza sativa ( indica
AAAA01004195

6.00E−69



cultivar-group)

184
G1136

Medicago

CB893334

1.00E−68




truncatula


184
G1136

Phaseolus vulgaris

gi1142619

2.00E−155

184
G1136

Oryza sativa

gi12643064

6.80E−128

184
G1136

Oryza sativa

gi31433653

6.80E−128



( japonica cultivar-



group)

184
G1136

Zea mays

gi4321762

2.30E−127

184
G1136

Lycopersicon

gi6175252

6.40E−58




esculentum


184
G1136

Petunia x hybrida

gi10998404

7.40E−36

184
G1136

Perilla frutescens

gi28375728

2.90E−34

184
G1136

Antirrhinum majus

gi166428

6.30E−26

184
G1136

Gossypium

gi13346182

4.60E−25




hirsutum


184
G1136

Gerbera hybrida

gi3650292

6.00E−19

185
G1142

Glycine max

GLYMA-28NOV01-
944




CLUSTER228631_1

185
G1142

Glycine max

GLYMA-28NOV01-
945




CLUSTER228631_2

185
G1142

Oryza sativa

ORYSA-22JAN02-
946




CLUSTER251531_1

185
G1142

Zea mays

ZEAMA-08NOV01-
947




CLUSTER137582_1

185
G1142

Zea mays

Zm_S11428899
1790

185
G1142

Lycopersicon

SGN-UNIGENE-
2012




esculentum

SINGLET-12268

186
G1142

Brassica oleracea

BZ072414

6.00E−71

186
G1142

Brassica napus

CD829735

4.00E−67

186
G1142

Populus tremula x

BU813371

3.00E−63




Populus tremuloides


186
G1142

Glycine max

BQ094663

2.00E−62

186
G1142

Medicago

BF647687

2.00E−60




truncatula


186
G1142

Populus tremula

BU891490

4.00E−55

186
G1142

Lycopersicon

AW622727

4.00E−43




esculentum


186
G1142

Oryza sativa

AK106649

1.00E−38



( japonica cultivar-



group)

186
G1142

Populus tremuloides

CA931085

2.00E−33

186
G1142
Oryza sativa subsp.
AU093196

2.00E−30




japonica


186
G1142

Oryza sativa

gi32129332

1.40E−39



( japonica cultivar-



group)

186
G1142

Oryza sativa gi8470062


2.70E−27

186
G1142

Pennisetum

gi527657

5.20E−07




glaucum


186
G1142

Phyllostachys acuta

gi527661

1.40E−06

186
G1142

Lycopersicon

gi617252

2.10E−06




esculentum


186
G1142

Sorghum bicolor

gi527665

2.00E−05

186
G1142

Oryza australiensis

gi1086526

2.40E−05

186
G1142

Oryza eichingeri

gi1086528

4.00E−05

186
G1142

Gossypium

gi13346180

6.60E−05




hirsutum


186
G1142

Oryza rufipogon

gi1086536

6.70E−05

188
G1150
Oryza sativa ( indica
AAAA01007141

1.00E−174



cultivar-group)

188
G1150

Oryza sativa

AC135597

1.00E−174



( japonica cultivar-



group)

188
G1150

Zea mays

AY109385

1.00E−157

188
G1150

Brassica oleracea

BH475694

1.00E−112

188
G1150

Triticum aestivum

BT008960

1.00E−103

188
G1150

Medicago

BI309506

4.00E−99




truncatula


188
G1150

Oryza sativa

BI118817

2.00E−81

188
G1150

Glycine max

BU761598

2.00E−80

188
G1150

Lycopersicon

BG125123

6.00E−69




esculentum


188
G1150

Solanum tuberosum

BG351593

9.00E−68

188
G1150

Oryza sativa

gi31712081

5.60E−170



( japonica cultivar-



group)

188
G1150

Oryza sativa

gi6539559

2.20E−97

188
G1150

Zea mays

gi8542175

8.40E−36

188
G1150

Pisum sativum

gi15021750

1.90E−05

188
G1150

Vicia faba

gi425682

6.60E−05

188
G1150

Phaseolus vulgaris

gi169349

0.0015

188
G1150

Adiantum capillus-

gi4033606

0.0019




veneris


188
G1150

Medicago

gi2598593

0.007




truncatula


188
G1150

Lycopersicon

gi170454

0.0072




esculentum


188
G1150

Solanum tuberosum

gi688080

0.011

189
G1206

Glycine max

GLYMA-28NOV01-
948




CLUSTER11328_1

189
G1206

Glycine max

GLYMA-28NOV01-
949




CLUSTER11328_2

189
G1206

Glycine max

GLYMA-28NOV01-
950




CLUSTER11328_4

189
G1206

Glycine max

GLYMA-28NOV01-
951




CLUSTER240450_1

189
G1206

Glycine max

GLYMA-28NOV01-
952




CLUSTER48180_5

189
G1206

Oryza sativa

15855173
953

189
G1206

Oryza sativa

AU172577.1
954

189
G1206

Oryza sativa

ORYSA-22JAN02-
955




CLUSTER100143_1

189
G1206

Oryza sativa

OSC101221.C1.p21.fg
956

189
G1206

Oryza sativa

OSC17045.C1.p1.fg
957

189
G1206

Oryza sativa

OSC24705.C1.p12.fg
958

189
G1206

Oryza sativa

OSC773.C1.p2.fg
959

189
G1206

Oryza sativa

rsicem_13769.y1.abd
960

189
G1206

Zea mays

LIB3912-033-Q6-K6-
961




A12

189
G1206

Zea mays

ZEAMA-08NOV01-
962




CLUSTER231432_1

189
G1206

Zea mays

ZEAMA-08NOV01-
963




CLUSTER30971_1

189
G1206

Zea mays

ZEAMA-08NOV01-
964




CLUSTER30971_3

189
G1206

Zea mays

ZEAMA-08NOV01-
965




CLUSTER73342_1

189
G1206

Zea mays

ZEAMA-08NOV01-
966




CLUSTER73342_2

189
G1206

Zea mays

ZEAMA-08NOV01-
967




CLUSTER998548_1

189
G1206

Glycine max

Gma_S4861534
1654

189
G1206

Glycine max

Gma_S4881720
1655

189
G1206

Glycine max

Gma_S5117867
1656

189
G1206

Medicago

Mtr_S5329905
1697




truncatula


189
G1206

Medicago

Mtr_S5408746
1698




truncatula


189
G1206

Zea mays

Zm_S11526858
1791

189
G1206

Lycopersicon

SGN-UNIGENE-48203
2013




esculentum


189
G1206

Lycopersicon

SGN-UNIGENE-
2014




esculentum

SINGLET-385084

189
G1206

Lycopersicon

SGN-UNIGENE-
2015




esculentum

SINGLET-463300

189
G1206

Lycopersicon

SGN-UNIGENE-
2016




esculentum

SINGLET-471325

190
G1206

Oryza sativa

AK059445

1.00E−133



(i japonica cultivar-



group)

190
G1206

Vicia sativa

VSENBP1GN

1.00E−123

190
G1206

Pisum sativum

PSPD3BIPR

1.00E−122

190
G1206

Brassica napus

CD815167

1.00E−109

190
G1206

Brassica oleracea

BH582293

1.00E−107

190
G1206

Mesembryanthemum

CA838861

2.00E−84




crystallinum


190
G1206

Solanum tuberosum

BQ507789

1.00E−74

190
G1206

Zea mays

AY106688

5.00E−74

190
G1206

Zinnia elegans

AU290850

5.00E−71

190
G1206

Medicago

AW775140

5.00E−70




truncatula


190
G1206

Vicia sativa

gi1360637

1.20E−172

190
G1206

Medicago

gi11358945

2.40E−169




truncatula


190
G1206

Pisum sativum

gi2213540

1.50E−166

190
G1206

Oryza sativa

gi21104742

0.083



( japonica cultivar-



group)

190
G1206

Medicago sativa

gi1279563

0.088

190
G1206

Nicotiana tabacum

gi8096269

0.29

190
G1206

Ananas comosus

gi31323668

0.38

190
G1206

Gossypium

gi11291753

0.83




hirsutum


190
G1206

Populus tremuloides

gi9651406

0.83

190
G1206

Cicer arietinum

gi3860319

0.94

192
G1247

Brassica oleracea

BZ440977

3.00E−31

192
G1247

Petunia x hybrida

PHMYBPH33

5.00E−28

192
G1247

Lactuca sativa

BQ996568

6.00E−28

192
G1247

Oryza sativa

AK063951

1.00E−27



( japonica cultivar-



group)

192
G1247

Oryza sativa

OSGAMYB

1.00E−27

192
G1247

Vitis vinifera

CB004589

1.00E−27

192
G1247

Lolium temulentum

AF114162

2.00E−27

192
G1247

Hordeum vulgare

HVRNAGAM1

2.00E−27

192
G1247

Triticum aestivum

AB044084

2.00E−27

192
G1247

Hordeum vulgare

CA013607

2.00E−27



subsp. vulgare

192
G1247

Triticum aestivum

gi8247759

9.60E−31

192
G1247

Oryza sativa

gi32489825

4.40E−29



( japonica cultivar-



group)

192
G1247

Zea mays

gi19072736

2.90E−28

192
G1247

Oryza sativa

gi1707640

1.80E−27

192
G1247

Sorghum bicolor

gi19073322

4.00E−27

192
G1247

Hordeum vulgare

gi1200239

5.50E−27

192
G1247

Petunia x hybrida

gi20565

8.30E−27

192
G1247

Lolium temulentum

gi4877649

1.30E−26

192
G1247

Lycopersicon

gi1430846

2.20E−26




esculentum


192
G1247

Nicotiana tabacum

gi11066263

3.70E−26

193
G1274

Glycine max

GLYMA-28NOV01-
968





CLUSTER16030_1

193
G1274

Glycine max

GLYMA-28NOV01-
969





CLUSTER305171_1

194
G1274

Oryza sativa

OSC100386.C1.p11.fg
970

194
G1274

Oryza sativa

OSC100526.C1.p1.fg
971

194
G1274

Zea mays

ZEAMA-08NOV01-
972




CLUSTER139642_1

194
G1274

Zea mays

ZEAMA-08NOV01-
973




CLUSTER139642_2

194
G1274

Zea mays

ZEAMA-08NOV01-
974




CLUSTER2967_14

194
G1274

Zea mays

ZEAMA-08NOV01-
975




CLUSTER452657_1

194
G1274

Lycopersicon

SGN-UNIGENE-51404
2017




esculentum


194
G1274

Lycopersicon

SGN-UNIGENE-57064
2018




esculentum


194
G1274

Glycine max

BQ742659

1.00E−33

194
G1274

Solanum tuberosum

BQ516647

2.00E−32

194
G1274

Lycopersicon

BI209002

2.00E−32




esculentum


194
G1274

Hordeum vulgare

BE216050

4.00E−31

194
G1274

Capsicum annuum

CA524920

2.00E−30

194
G1274

Stevia rebaudiana

BG525040

3.00E−30

194
G1274

Sorghum bicolor

CD233113

3.00E−29

194
G1274

Zea mays

BM334368

2.00E−28

194
G1274

Hordeum vulgare

BJ478103

3.00E−28



subsp. spontaneum

194
G1274

Hordeum vulgare

BJ456908

3.00E−28



subsp. vulgare

194
G1274

Oryza sativa

gi9558431

1.10E−28

194
G1274

Oryza sativa

gi21104763

4.90E−28



( japonica cultivar-



group)

194
G1274

Nicotiana tabacum

gi29536791

6.00E−23

194
G1274

Capsella rubella

gi32454266

1.70E−22

194
G1274

Solanum tuberosum

gi24745606

8.70E−22

194
G1274
Oryza sativa ( indica
gi23305051

1.40E−21



cultivar-group)

194
G1274

Pimpinella

gi3420906

1.70E−21




brahycarpa


194
G1274

Lycopersicon

gi13620227

3.90E−21




esculentum


194
G1274

Cucumis sativus

gi7484759

5.70E−21

194
G1274

Ipomoea batatas

gi1076685

7.00E−21

195
G1276

Medicago

Mtr_S7094215
1699




truncatula


195
G1276

Lycopersicon

SGN-UNIGENE-
2019




esculentum

SINGLET-449521

195
G1276

Hyacinthus

AF134116

5.00E−68




orientalis


195
G1276

Pisum sativum

AF325506

2.00E−63

195
G1276

Brassica oleracea

BH541466

4.00E−62

195
G1276

Antirrhinum majus

AY223518

9.00E−62

196
G1276

Petunia x hybrida

AF132002

9.00E−62

196
G1276

Zea mays

AF048900

5.00E−61

196
G1276

Malus x domestica

AF332215

2.00E−60

196
G1276

Hordeum vulgare

AY069953

9.00E−60

196
G1276

Glycine max

CA783794

3.00E−59

196
G1276

Lycopersicon

BI933811

3.00E−59




esculentum


196
G1276

Hyacinthus

gi5360996

1.00E−66




orientalis


196
G1276

Pisum sativum

gi13173164

6.60E−65

196
G1276

Antirrhinum majus

gi28894443

2.20E−62

196
G1276

Zea mays

gi2944040

3.60E−62

196
G1276

Hordeum vulgare

gi18476518

1.60E−61

196
G1276

Petunia x hybrida

gi5081557

5.40E−61

196
G1276

Oryza sativa

gi24059986

2.30E−60



( japonica cultivar-



group)

196
G1276

Malus x domestica

gi21717332

7.30E−57

196
G1276

Picea abies

gi11181610

4.00E−56

196
G1276

Fragaria x

gi30314933

1.30E−36




ananassa


198
G1289

Oryza sativa

AK069906

1.0e−999



( japonica cultivar-



group)

198
G1289

Zea mays

AY104480

1.00E−177

198
G1289

Brassica napus

CD842929

1.00E−124

198
G1289

Oryza sativa

CNS08C8Z

1.00E−123

198
G1289
Oryza sativa ( indica
AAAA01003696

1.00E−123



cultivar-group)

198
G1289

Malus domestica

AF220204

1.00E−106

198
G1289

Descurainia sophia

BU238049

1.00E−104

198
G1289

Vitis vinifera

CB974203

1.00E−100

198
G1289

Lactuca sativa

BQ861345

2.00E−95

198
G1289

Glycine max

BI786110

1.00E−90

198
G1289

Oryza sativa

gi15624052

1.60E−176



( japonica cultivar-



group)

198
G1289

Oryza sativa

gi15408875

6.30E−122

198
G1289

Malus x domestica

gi6752888

5.90E−103

198
G1289

Narcissus

gi18419598

3.60E−24




pseudonarcissus


198
G1289

Pinus pinaster

gi20218829

4.70E−09

198
G1289

Lycopersicon

gi15144514

0.0032




esculentum


198
G1289

Prunus persica

gi27450532

0.0035

198
G1289

Ricinus communis

gi2246458

0.0043

198
G1289

Nicotiana tabacum

gi17044043

0.0055

198
G1289

Glycine max

gi1399380

0.013

199
g1313

Glycine max

GLYMA-028NOV01-
976




CLUSTER110578_1

199
G1313

Oryza sativa

ORYSA-22JAN02-
977




CLUSTER1200_1

199
G1313

Oryza sativa

OSC10811.C1.p1.fg
978

199
G1313

Oryza sativa

OSC102408.C1.p25.fg
979

199
G1313

Zea mays

ZEAMA-08NOV01-
980




CLUSTER163873_1

199
G1313

Zea mays

ZEAMA-08NOV01-
981




CLUSTER163873_4

199
G1313

Oryza sativa

Os_S60589
1582

199
G1313

Oryza sativa

Os_S60753
1583

199
G1313

Hordeum vulgare

Hv_S73931
1734

200
G1313

Hordeum vulgare

HVRNAGAM1

4.00E−72

200
G1313

Lolium temulentum

AF114162

7.00E−72

200
G1313

Oryza sativa

AK063951

4.00E−71



( japonica cultivar-



group)

200
G1313

Oryza sativa

AX699725

4.00E−71

200
G1313

Avena sativa

ASA133638

4.00E−71

200
G1313

Vitis vinifera

CB005976

3.00E−66

200
G1313

Hordeum vulgare

CA014662

2.00E−58



subsp. vulgare

200
G1313

Triticum aestivum

BQ245589

8.00E−58

200
G1313

Zea mays

CD439889

3.00E−55

200
G1313

Petunia x hybrida

PHMYBPH33

7.00E−54

200
G1313

Hordeum vulgare

gi13236696

4.00E−72

200
G1313

Oryza sativa

gi18844771

8.40E−72



( japonica cultivar-



group)

200
G1313

Oryza sativa

gi1707640

1.40E−71

200
G1313

Lolium temulentum

gi4877649

2.20E−71

200
G1313

Avena sativa

gi4581969

1.60E−70

200
G1313

Triticum aestivum

gi8247759

1.40E−55

200
G1313

Petunia x hybrida

gi20565

3.80E−53

200
G1313

Nicotiana tabacum

gi11066265

2.60E−52

200
G1313

Lotus corniculatus

gi30024602

2.40E−41



var. japonicus

200
G1313

Glycine max

gi30024604

1.50E−40

202
G1327

Oryza sativa

AX652823

1.00E−52

202
G1327
Oryza sativa ( indica
CB619057

3.00E−52



cultivar-group)

202
G1327

Petunia x hybrida

PHMYBPH22

4.00E−52

202
G1327

Brassica napus

CD834677

1.00E−50

202
G1327

Glycine max

AB029160

6.00E−50

202
G1327

Sorghum bicolor

CD214131

8.00E−50

202
G1327

Triticum aestivum

BJ312394

8.00E−50

202
G1327

Hordeum vulgare

BG343209

8.00E−50

202
G1327

Nicotiana tabacum

AB028650

8.00E−50

202
G1327

Solanum tuberosum

BQ514539

1.00E−49

202
G1327

Petunia x hybrida

gi20561

1.10E−51

202
G1327

Oryza sativa

gi33087073

2.40E−51



( japonica cultivar-



group)

202
G1327

Zea mays

gi127580

6.30E−51

202
G1327

Oryza sativa

gi1946265

1.00E−50

202
G1327

Nicotiana tabacum

gi6552361

1.30E−50

202
G1327

Glycine max

gi5139802

1.20E−49

202
G1327

Vitis labrusca x

gi22266675

3.10E−49




Vitis vinifera


202
G1327

Lycopersicon

gi1370140

4.00E−49




esculentum


202
G1327

Sorghum bicolor

gi19548405

5.10E−49

202
G1327

Populus x

gi22795039

3.60E−48




canescens


204
G1340

Brassica oleracea

BZ504572

5.00E−64

204
G1340

Populus tremula x

BU832210

7.00E−56




Populus tremuloides


204
G1340

Gossypium

BQ407507

1.00E−53




arboreum


204
G1340

Oryza sativa

AC135792

1.00E−51



( japonica cultivar-



group)

204
G1340
Oryza sativa ( indica
AAAA01006150

3.00E−47



cultivar-group)

204
G1340

Lotus corniculatus

AP006423

2.00E−41



var. japonicus

204
G1340

Oryza sativa

OSIG00051

1.00E−40

204
G1340

Solanum tuberosum

BQ510169

3.00E−37

204
G1340

Zea mays

CC658096

1.00E−34

204
G1340

Sorghum bicolor

BZ346856

7.00E−29

204
G1340

Oryza sativa

gi32488407

3.20E−56



( japonica cultivar-



group)

204
G1340

Lycopersicon

gi1345538

0.00087




esculentum


204
G1340

Oryza sativa

gi13786451

0.0023

204
G1340

Glycine max

gi18182309

0.0075

204
G1340

Triticum aestivum

gi5292165

0.0099

204
G1340

Nicotiana tabacum

gi6691123

0.011

204
G1340

Solanum tuberosum

gi688080

0.013

204
G1340

Volvox carteri

gi21992

0.023

204
G1340

Vicia faba

gi425682

0.03

204
G1340

Bromheadia

gi2108258

0.046




finlaysoniana


206
G1341

Gossypium

AY125487

1.0e−999




hirsutum


206
G1341

Oryza sativa

AF268596

1.00E−103

206
G1341

Oryza sativa

AK064970

1.00E−101



( japonica cultivar-



group)

206
G1341

Poncirus trifoliata

CD575941

5.00E−95

206
G1341

Medicago

CF068570

5.00E−90




truncatula


206
G1341
Oryza sativa ( indica
AAAA01000469

1.00E−88



cultivar-group)

206
G1341

Helianthus annuus

CD852109

1.00E−85

206
G1341

Zea mays

BZ968534

2.00E−72

206
G1341

Triticum aestivum

BJ301302

2.00E−69

206
G1341

Brassica napus

CD825286

2.00E−61

206
G1341

Gossypium

gi22858664

3.10E−191




hirsutum


206
G1341

Oryza sativa

gi13124871

1.80E−95

206
G1341

Oryza sativa

gi31433523

1.80E−95



( japonica cultivar-



group)

206
G1341

Marsilea

gi22550110

4.50E−24




quadrifolia


206
G1341

Volvox carteri

gi226743

1.20E−12

206
G1341

Volvox carteri f.

gi6523547

2.10E−12




nagariensis


206
G1341

Lupinus

gi28912428

1.10E−11




angustifolius


206
G1341

Lycoperison

gi7488999

1.30E−10




esculentum


206
G1341

Adiantum capillus-

gi4033606

2.80E−10




veneris


206
G1341

Cicer arietinum

gi3204132

3.50E−10

207
G1357

Glycine max

GLYMA-28NOV01-
982




CLUSTER80398_1

207
G1357

Lycopersicon

SGN-UNIGENE-52387
2020




esculentum


208
G1357

Brassica oleracea

BH590226

3.00E−94

208
G1357

Medicago

BF645605

5.00E−59




truncatula


208
G1357

Sorghum bicolor

BI140703

8.00E−44

208
G1357

Hordeum vulgare

BJ481205

8.00E−44



subsp. spontaneum

208
G1357

Hordeum vulgare

BU967516

8.00E−44



subsp. vulgare

208
G1357

Hordeum vugare

BQ469035

8.00E−44

208
G1357

Petunia x hybrida

AF509874

9.00E−42

208
G1357

Triticum aestivum

BJ257015

9.00E−42

208
G1357

Oryza sativa

AX654515

3.00E−41

208
G1357

Oryza sativa

AK099540

5.00E−41



( japonica cultivar-



group)

208
G1357

Oryza sativa

gi9225018

1.50E−42



( japonica cultivar-



group)

208
G1357

Petunia x hybrida

gi21105751

2.40E−42

208
G1357

Medicago

gi7716952

7.20E−42




truncatula


208
G1357

Oryza sativa

gi6730946

3.50E−41

208
G1357

Glycine max

gi22597158

1.10E−37

208
G1357

Brassica napus

gi31322582

4.30E−36

208
G1357

Phaseolus vulgaris

gi15148914

7.00E−36

208
G1357

Lycopersicon

gi6175246

2.20E−32




esculentum


208
G1357
Triticum sp.
gi4218537

2.80E−32

208
G1357

Triticum

gi6732160

2.80E−32




monococcum


210
G1361

Oryza sativa

AK102794

1.00E−103



( japonica cultivar-



group)

210
G1361

Brassica oleracea

BH541034

7.00E−97

210
G1361

Brassica napus

AF319771

1.00E−96

210
G1361

Hordeum vulgare

BI960052

5.00E−90

210
G1361

Populus tremula x

BI129724

2.00E−88




Populus tremuloides


210
G1361
Oryza sativa ( indica
AAAA01011028

4.00E−86



cultivar-group)

210
G1361

Glycine max

BM527360

4.00E−86

210
G1361

Zea mays

CC340048

3.00E−84

210
G1361

Medicago

BG646679

2.00E−83




truncatula


210
G1361

Sorghum bicolor

CD235886

2.00E−72

210
G1361

Oryza sativa

gi18461166

3.10E−97



( japonica cultivar-



group)

210
G1361

Brassica napus

gi12751304

1.30E−91

210
G1361

Oryza sativa

gi9049470

2.20E−70

210
G1361

Triticum

gi6732156

6.10E−12




monococcum


210
G1361

Lycopersicon

gi6175246

3.40E−11




esculentum


210
G1361

Glycine max

gi22597158

2.90E−10

210
G1361
Triticum sp.
gi4218537

8.10E−10

210
G1361

Phaseolus vulgaris

gi15148914

7.40E−09

210
G1361

Petunia x hybrida

gi1279640

1.00E−08

210
G1361

Medicago

gi7716952

2.20E−08




truncatula


212
G1361

Solanum tuberosum

BQ115095

7.00E−49

212
G1361

Lycopersicon

AF506825

7.00E−49




esculentum


212
G1384

Brassica oleracea

BH517407

1.00E−46

212
G1384

Brassica napus

CD829462

9.00E−46

212
G1384

Prunus persica

BU046010

2.00E−45

212
G1384

Hedyotis

CB083964

2.00E−44




centranthoides


212
G1384

Atriplex hortensis

AF274033

5.00E−44

212
G1384

Oryza sativa

AK105877

6.00E−44



( japonica cultivar-



group)

212
G1384

Oryza sativa

AP004119

6.00E−44

212
G1384

Glycine max

BE807772

1.00E−43

212
G1384

Lycopersicon

gi27436378

1.50E−49




esculentum


212
G1384

Oryza sativa

gi32140997

1.90E−43



( japonica cultivar-



group)

212
G1384

Zea mays

gi21908036

4.50E−41

212
G1384

Atriplex hortensis

gi8571476

4.20E−38

212
G1384

Oryza sativa

gi5091503

3.60E−22

212
G1384

Nicotiana tabacum

gi1208498

1.00E−20

212
G1384

Prunus armeniaca

gi3264767

1.70E−20

212
G1384

Mesembryanthemum

gi32401273

1.50E−19




crystallinum


212
G1384

Solanum tuberosum

gi28268684

5.00E−19

212
G1384

Glycine max

gi31324058

1.00E−18

212
G1384

Brassica oleracea

BH720211

2.00E−96

214
G1389

Medicago

AC144893

8.00E−47




truncatula


214
G1389

Lotus japonicus

AP006146

3.00E−46

214
G1389

Lactuca sativa

BQ996587

3.00E−39

214
G1389

Lupinus albus

LAL426419

8.00E−39

214
G1389

Glycine max

AW760150

2.00E−38

214
G1389

Oryza sativa

AB071805

3.00E−38



( japonica cultivar-



group)

214
G1389
Oryza sativa ( indica
AAAA01005156

3.00E−38



cultivar-group)

214
G1389

Oryza sativa

AP003104

3.00E−38

214
G1389

Gossypium

BG440212

5.00E−38




arboreum


214
G1389

Oryza sativa

gi20975253

3.00E−45



( japonica cultivar-



group)

214
G1389

Lupinus albus

gi20269127

2.80E−44

214
G1389

Oryza sativa

gi14164473

1.20E−43

214
G1389

Lycopersicon

gi12002867

1.90E−39




esculentum


214
G1389

Pueraria montana

gi21624281

6.10E−21



var. lobata

214
G1389

Digitalis purpurea

gi6358561

8.30E−21

214
G1389

Misopates orontium

gi6358605

1.50E−20

214
G1389

Antirrhinum majus

gi6358551

2.80E−20



subsp. linkianum

214
G1389

Antirrhinum

gi32481583

3.40E−20




microphyllum


214
G1389

Antirrhinum

gi6358548

3.40E−20




graniticum


215
G1412

Glycine max

GLYMA-28NOV01-
983




CLUSTER16227_1

215
G1412

Glycine max

GLYMA-28NOV01-
984




CLUSTER16227_2

215
G1412

Glycine max

GLYMA-28NOV01-
985




CLUSTER16227_3

215
G1412

Glycine max

GLYMA-28NOV01-
986




CLUSTER16227_5

215
G1412

Glycine max

GLYMA-28NOV01-
987




CLUSTER444_125

215
G1412

Glycine max

GLYMA-28NOV01-
988




CLUSTER444_267

215
G1412

Glycine max

GLYMA-28NOV01-
989




CLUSTER444_320

215
G1412

Glycine max

GLYMA-28NOV01-
990




CLUSTER444_337

215
G1412

Zea mays

ZEAMA-08NOV01-
991




CLUSTER721_119

215
G1412

Glycine max

Gma_S5050636
1657

215
G1412

Lycopersicon

Les_S5295623
1930




esculentum


215
G1412

Lycopersicon

SGN-UNIGENE-45948
2021




esculentum


215
G1412

Lycopersicon

SGN-UNIGENE-48215
2022




esculentum


216
G1412

Brassica napus

AY245887

1.00E−149

216
G1412

Citrus sinensis

CB290927

1.00E−106

216
G1412

Lycopersicon

AF011555

1.00E−103




esculentum


216
G1412

Mesembryanthemum

BE034140

1.00E−100




crystallinum


216
G1412

Beta vulgaris

BQ586991

5.00E−98

216
G1412

Solanum tuberosum

BQ516602

1.00E−96

216
G1412

Lactuca sativa

BQ854150

2.00E−95

216
G1412

Medicago

Aj498717

4.00E−94




truncatula


216
G1412

Vitis vinifera

CB915147

2.00E−93

216
G1412

Glycine max

BG510868

7.00E−84

216
G1412

Brassica napus

gi31322582

2.00E−141

216
G1412

Lycopersicon

gi6175246

2.70E−98




esculentum


216
G1412

Oryza sativa

gi15528779

1.80E−62

216
G1412

Oryza sativa

gi20161457

8.80E−61



( japonica cultivar-



group)

216
G1412

Petunia x hybrida

gi21105748

2.30E−60

216
G1412

Solanum tuberosum

gi14485513

9.10E−59

216
G1412
Triticum sp.
gi4218535

1.30E−57

216
G1412

Triticum

gi6732158

1.30E−57




monococcum


216
G1412

Phaseolus vulgaris

gi15148914

4.60E−55

216
G1412

Glycine max

gi22597158

1.70E−50

217
G1420

Glycine max

GLYMA-28NOV01-
992




CLUSTER227245_1

217
G1420

Glycine max

GLYMA-28NOV01-
993




CLUSTER90086_1

217
G1420

Zea mays

ZEAMA-08NOV01-
994




CLUSTER22982_1

217
G1420

Zea mays

ZEAMA-08NOV01-
995




CLUSTER453197_1

218
G1420

Brassica rapa

L35779

1.00E−62

218
G1420

Vitis vinifera

BQ799236

2.00E−55

218
G1420

Glycine max

BF425463

1.00E−50

218
G1420

Brassica oleracea

BH451149

2.00E−47

218
G1420

Populus tremula x

BU884581

1.00E−44




Populus tremuloides


218
G1420

Lycopoersicon

AQ034229

3.00E−44




esculentum


218
G1420

Amborella

CD483414

2.00E−42




trichopoda


218
G1420

Oryza sativa

AK108745

8.00E−40



( japonica cultivar-



group)

218
G1420

Medicago

BF645445

2.00E−39




truncatula


218
G1420

Oryza sativa

AX654272

6.00E−39

218
G1420

Oryza sativa

gi11761085

7.40E−41

218
G1420

Oryza sativa

gi22830985

3.90E−35



( japonica cultivar-



group)

218
G1420

Lycopersicon

gi13620227

1.40E−33




esculentum


218
G1420

Nicotiana tabacum

gi14530681

4.40E−32

218
G1420

Capsella rubella

gi32454266

5.70E−32

218
G1420

Pimpinella

gi3420906

5.80E−30




brachycarpa


218
G1420

Petroselinum

gi5917653

2.90E−28




crispum


218
G1420

Avena fatua

gi1159877

5.60E−27

218
G1420

Solanum tuberosum

gi24745606

5.60E−27

218
G1420

Retama raetam

gi18158619

3.10E−24

220
G1423

Brassica oleracea

BH449565

1.00E−53

220
G1423

Lycopersicon

BH449565

2.00E−10




esculentum


220
G1423

Medicago

AC139746

1.00E−08




truncatula


220
G1423

Lotus corniculatus

CB827123

1.00E−08



var. japonicus

220
G1423

Lotus japonicus

AP006142

1.00E−08

220
G1423

Oryza sativa

CNS07YPL
1.00E−07

220
G1423
Oryza sativa ( indica
AAAA01005416

3.00E−07



cultivar-group)

220
G1423

Brassica napus

CD815249

3.00E−07

220
G1423

Sorghum bicoor

BZ627051

7.00E−07

220
G1423

Gossypium

BQ412101

7.00E−07




arboreum


220
G1423

Oryza sativa

gi15290141

3.80E−06

220
G1423

Oryza sativa

ge30313673

4.30E−06



( japonica cultivar-



group)

220
G1423

Brassica oleracea

gi30523252

4.50E−05



var. capitata

220
G1423

Zea mays

gi29372756

4.90E−05

220
G1423

Raphanus sativus

gi30523250

5.10E−05

220
G1423

Ceratopteris

gi1944532

5.90E−05




richardii


220
G1423

Brassica napus

gi17933454

6.00E−05

220
G1423

Brassica rapa

gi30523360

6.00E−05

220
G1423

Petunia x hybrida

gi13384062

6.20E−05

220
G1423

Hordeum vulgare

gi9367234

8.70E−05

222
G1446

Brassica rapa

AC137926

1.00E−113

222
G1446

Brassica oleracea

BH602216

3.00E−66

222
G1446

Lotus japonicus

AP004983

8.00E−37

222
G1446

Medicago

BI309565

2.00E−25




truncatula


222
G1446

Lactuca sativa

VU005471

2.00E−19

222
G1446

Glycine max

BM525749

2.00E−18

222
G1446

Lycopersicon

BI934904

2.00E−18




esculentum


222
G1446

Oryza sativa

AC137991

3.00E−17



( japonica cultivar-



group)

222
G1446
Oryza sativa ( indica
AAAA01014243

4.00E−17



cultivar-group)

222
G1446

Solanum tuberosum

BG594881

2.00E−16

222
G1446

Oryza sativa

gi21104742

0.00012



( japonica cultivar-



group)

222
G1446

Nicotiana tabacum

gi8096269

0.0003

222
G1446

Raphanus sativus

gi9049359

0.0012

222
G1446
Oryza sativa ( indica
gi23345287

0.0016



cultivar-group)

222
G1446

Oryza sativa

gi3885888

0.0016

222
G1446

Boea crassifolia

gi13992713

0.0023

222
G1446

Phaseolus vulgaris

gi1326161

0.0037

222
G1446

Zea mays

gi123378

0.017

222
G1446

Cucurbita maxima

gi17221648

0.023

223
G1451

Lycopersicon

gi135171

0.051




esculentum


223
G1451

Glycine max

GLYMA-28NOV01-
996




CLUSTER34672_1

223
G1451

Glycine max

GLYMA-28NOV01-
997




CLUSTER34672_2

223
G1451

Oryza sativa

ORYSA-22JAN02-
998




CLUSTER11490_1

223
G1451

Oryza sativa

ORYSA-22JAN02-
999




CLUSTER2788_1

223
G1451

Oryza sativa

ORYSA-22JAN02-
1000




CLUSTER294601_1

223
G1451

Oryza sativa

OSC100137.C1.p3.fg
1001

223
G1451

Oryza sativa

OSC100815.C1.p16.fg
1002

223
G1451

Oryza sativa

OSC17225.C1.p4.fg
1003

223
G1451

Oryza sativa

OSC21362.C1.p3.fg
1004

223
G1451

Zea mays

ZEAMA-08NOV01-
1005




CLUSTER16365_1

223
G1451

Zea mays

ZEAMA-08NOV01-
1006




CLUSTER16500_1

223
G1451

Zea mays

ZEAMA-08NOV01-
1007




CLUSTER36166_1

223
G1451

Zea mays

ZEAMA-08NOV01-
1008




CLUSTER9201_1

223
G1451

Oryza sativa

Os_S113016
1584

223
G1451

Oryza sativa

Os_S113019
1585

223
G1451

Oryza sativa

Os_S113021
1586

223
G1451

Glycine max

Gma_S4864163
1658

223
G1451

Glycine max

Gma_S4867879
1659

223
G1451

Medicago

Mtr_S5443860
1700




truncatula


223
G1451

Hordeum vulgare

Hv_S73176
1735

223
G1451

Zea mays

Zm_S11325188
1792

223
G1451

Zea mays

Zm_S11325542
1793

223
G1451

Zea mays

Zm_S11486245
1794

223
G1451

Zea mays

Zm_S11521797
1795

223
G1451

Zea mays

Zm_S11523792
1796

223
G1451

Zea mays

Zm_S11526178
1797

223
G1451

Triticum aestivum

Ta_S132434
1864

223
G1451

Triticum aestivum

Ta_S133054
1865

223
G1451

Triticum aestivum

Ta_S142068
1866

223
G1451

Lycopersicon

Les_S5190833
1931




esculentum


223
G1451

Lycopersicon

SGN-UNIGENE-47770
2023




esculentum


223
G1451

Lycopersicon

SGN-UNIGENE-49633
2024




esculentum


223
G1451

Lycopersicon

SGN-UNIGENE-52521
2025




esculentum


223
G1451

Lycopersicon

SGN-UNIGENE-56226
2026




esculentum


223
G1451

Lycopersicon

SGN-UNIGENE-58048
2027




esculentum


223
G1451

Lycopersicon

SGN-UNIGENE-
2028




esculentum

SINGLET-339069

223
G1451

Lycopersicon

SGN-UNIGENE-
2029




esculentum

SINGLET-360353

223
G1451

Lycopersicon

SGN-UNIGENE-
2030




esculentum

SINGLET-62180

224
G1451

Oryza sativa

AB071298

1.0e−999

224
G1451

Oryza sativa

AK071455

1.0e−999



( japonica cultivar-



group)

224
G1451

Zea mays

AY105215

1.00E−157

224
G1451

Medicago

CB894037

1.00E−110




truncatula


224
G1451

Poncirus trifoliata

CD576399

1.00E−109

224
G1451

Lactuca sativa

BQ862285

1.00E−107

224
G1451

Solanum tuberosum

BG597435

1.00E−107

224
G1451

Triticum aestivum

VJ303602

1.00E−103

224
G1451

Mangifera indica

AY255705

5.00E−99

224
G1451

Populus tremuloides

CA930279

2.00E−92

224
G1451

Oryza sativa

gi32488726

2.20E−247



( japonica cultivar-



group)

224
G1451

Oryza sativa

gi19352049

4.50E−247

224
G1451

Prunus persica

gi27450533

2.20E−159

224
G1451
Oryza sativa ( indica
gi26251300

2.20E−116



cultivar-group)

224
G1451

Mangifera indica

gi30027167

4.10E−115

224
G1451

Mirabilis jalapa

gi23343944

2.90E−28

224
G1451

Marchantia

gi25272004

2.30E−12




polymorpha


224
G1451

Populus tremula x

gi20269053

8.60E−10




Populus tremuloides


224
G1451

Vigna radiata

gi287566

3.80E−06

224
G1451

Glycine max

gi114733

1.40E−05

225
G1452

Glycine max

GLYMA-28NOV01-
982




CLUSTER80398_1

225
G1452

Lycopersicon

SGN-UNIGENE-52387
2020




esculentum


226
G1452

Medicago

BF645605

5.00E−65




truncatula


226
G1452

Sorghum bicolor

BI140703

7.00E−43

226
G1452

Hordeum vulgare

BQ469035

1.00E−42

226
G1452

Hordeum vulgare

BU967516

1.00E−42



subsp. vulgare

226
G1452

Hordeum vulgare

BJ481205

1.00E−42



subsp. spontaneum

226
G1452

Triticum aestivum

BQ620568

3.00E−42

226
G1452
Oryza sativa ( indica
CB630990

3.00E−42



cultivar-group)

226
G1452

Oryza sativa

AX654172

8.00E−42

226
G1452

Oryza sativa

CB657109

1.00E−41



( japonica cultivar-



group)

226
G1452

Lactuca sativa

BQ997138

4.00E−41

226
G1452

Oryza sativa

gi6730946

1.30E−44

226
G1452

Petunia x hybrida

gi21105746

1.20E−41

226
G1452

Oryza sativa

gi27452910

1.30E−44



( japonica cultivar-



group)

226
G1452

Medicago

gi7716952

5.80E−41




truncatula


226
G1452

Glycine max

gi22597158

5.30E−38

226
G1452

Phaseolus vulgaris

gi15148914

7.00E−36

226
G1452

Brassica napus

gi31322578

2.30E−35

226
G1452
Triticum sp.
gi4218537

3.90E−35

226
G1452

Triticum

gi6732160

3.90E−35




monococcum


226
G1452

Lycopersicon

gi6175246

7.20E−34




esculentum


227
G1468

Glycine max

GLYMA-28NOV01-
1009




CLUSTER310714_1

227
G1468

Oryza sativa

OSC100112.C1.p1.fg
1010

227
G1468

Oryza sativa

OSC100807.C1.p13.fg
1011

227
G1468

Oryza sativa

OSC101260.C1.p1.fg
1012

227
G1468

Oryza sativa

Os_S109216
1587

227
G1468

Oryza sativa

Os_S114084
1588

227
G1468

Oryza sativa

Os_S16324
1589

227
G1468

Oryza sativa

Os_S76625
1590

227
G1468

Oryza sativa

Os_S79274
1591

227
G1468

Glycine max

Gma_S5105862
1660

227
G1468

Glycine max

Gma_S5112587
1661

227
G1468

Medicago

Mtr_S5310408
1701




truncatula


227
G1468

Medicago

Mtr_S5369405
1702




truncatula


227
G1468

Hordeum vulgare

Hv_S19455
1736

227
G1468

Hordeum vulgare

Hv_S206003
1737

227
G1468

Hordeum vulgare

Hv_S30762
1738

227
G1468

Zea mays

Zm_S11376904
1798

227
G1468

Zea mays

Zm_S11394555
1799

227
G1468

Zea mays

Zm_S11433720
1800

227
G1468

Zea mays

Zm_S11525354
1801

227
G1468

Triticum aestivum

Ta_S124759
1867

227
G1468

Triticum aestivum

Ta_S126524
1868

227
G1468

Triticum aestivum

Ta_S170737
1869

227
G1468

Triticum aestivum

Ta_S306243
1870

227
G1468

Triticum aestivum

Ta_S318815
1871

227
G1468

Triticum aestivum

Ta_S318948
1872

227
G1468

Triticum aestivum

Ta_S324081
1873

227
G1468

Triticum aestivum

Ta_S327698
1874

227
G1468

Triticum aestivum

Ta_S331818
1875

227
G1468

Triticum aestivum

Ta_S347368
1876

227
G1468

Triticum aestivum

Ta_S51995
1877

227
G1468

Triticum aestivum

Ta_S62789
1878

227
G1468

Lycopersicon

SGN-UNIGENE-58660
2031




esculentum


228
G1468

Brassica oleracea

BH517020

2.00E−98

228
G1468

Petunia x hybrida

AB000453

1.00E−37

228
G1468

Oryza sativa

AK069623

2.00E−27



( japonica cultivar-



group)

228
G1468
Oryza sativa ( indica
AAAA01005957

5.00E−27



cultivar-group)

228
G1468

Oryza sativa

OSJN00060

1.00E−26

228
G1468

Zea mays

CC671589

4.00E−23

228
G1468

Datisca glomerata

AF119050

5.00E−19

228
G1468

Vitis aestivalis

CB289561

1.00E−18

228
G1468

Vitis vinifera

BM437679

2.00E−18

228
G1468

Medicago

AC126007

2.00E−18




truncatula


228
G1468

Petunia x hybrida

gi1786138

1.00E−36

228
G1468

Oryza sativa

gi32482980

2.30E−30



( japonica cultivar-



group)

228
G1468

Datisca glomerata

gi4666360

3.50E−21

228
G1468

Glycine max

gi1763063

6.70E−20

228
G1468

Pisum sativum

gi2129892

6.50E−17

228
G1468

Nicotiana tabacum

gi2981169

1.10E−16

228
G1468

Oryza sativa

gi15623826

5.70E−12

228
G1468

Brassica rapa

gi2058504

8.30E−11

228
G1468

Triticum aestivum

gi485814

2.10E−10

228
G1468

Medicago sativa

gi7228329

3.00E−08

230
G1474

Brassica oleracea

BZ030450

1.00E−78

230
G1474

Medicago

AC135233

1.00E−25




truncatula


230
G1474
Oryza sativa ( indica
AAAA01001411

2.00E−22



cultivar-group)

230
G1474

Oryza sativa

AP005869

6.00E−22



( japonica cultivar-



group)

230
G1474

Hordeum vulgare

CG308988

1.00E−20

230
G1474

Zea mays

CC652748

7.00E−20

230
G1474

Glycine max

BG363109

3.00E−19

230
G1474

Oryza sativa

AC133008

3.00E−19

230
G1474

Helianthus annuus

BQ977193

8.00E−18

230
G1474

Brassica rapa

BZ614339

2.00E−14



subsp. pekinensis

230
G1474

Oryza sativa

gi32489630

5.50E−20



( japonica cultivar-



group)

230
G1474

Zea ramosa

gi18674684

3.40E−12

230
G1474

Petunia x hybrida

gi14275902

2.40E−10

230
G1474

Oryza sativa

gi15528588

3.90E−05

230
G1474

Sorghum bicolor

gi18390109

0.00065

230
G1474

Medicago sativa

gi7228329

0.022

230
G1474

Nicotiana tabacum

gi2981169

0.081

230
G1474

Glycine max

gi1763063

0.11

230
G1474

Brassica rapa

gi2058506

0.17

230
G1474

Pisum sativum

gi2129892

0.21

231
G1476

Oryza sativa

OSC101556.C1.p17.fg
1013

232
G1476

Brassica oleracea

BZ450400

2.00E−51

232
G1476

Lotus japonicus

AP006103

2.00E−11

232
G1476

Lotus corniculatus

CB826842

9.00E−11



var. japonicus

232
G1476

Medicago

AC145202

9.00E−11




truncatula


232
G1476

Oryza sativa (
indica

AAAA01025258

2.00E−10



( japonica cultivar-



group)

232
G1476

Oryza sativa

AP004336

2.00E−10



cultivar-group)

232
G1476

Zea mays

BH871177

4.00E−09

232
G1476

Oryza sativa

AP004020

1.00E−08

232
G1476

Petunia x hybrida

AB035093

1.00E−08

232
G1476

Glycine max

AI973860

9.00E−08

232
G1476

Oryza sativa

gi27261062

2.30E−14



( japonica cultivar-



group)

232
G1476

Petunia x hybrida

gi14275902

2.90E−14

232
G1476

Zea ramosa

gi18674684

1.70E−09

232
G1476

Oryza sativa

gi9558464

1.60E−07

232
G1476

Sorghum bicolor

gi18390109

5.30E−06

232
G1476

Datisca glomerata

gi4666360

0.0047

232
G1476

Pisum sativum

gi2129892

0.0057

232
G1476

Medicago sativa

gi7228329

0.011

232
G1476

Brassica rapa

gi2058504

0.011

232
G1476

Nicotiana tabacum

gi2981169

0.02

233
G1482

Glycine max

GLYMA-28NOV01-
1014




CLUSTER228559_1

233
G1482

Glycine max

GLYMA-28NOV01-
1015




CLUSTER228559_2

233
G1482

Glycine max

GLYMA-28NOV01-
1016




CLUSTER38097_1

233
G1482

Glycine max

GLYMA-28NOV01-
1017




CLUSTER39971_1

233
G1482

Glycine max

GLYMA-28NOV01-
1018




CLUSTER39971_2

233
G1482

Oryza sativa

ORYSA-22JAN02-
1019




CLUSTER17570_1

233
G1482

Oryza sativa

ORYSA-22JAN02-
1020




CLUSTER17570_2

233
G1482

Oryza sativa

ORYSA-22JAN02-
1021




CLUSTER687_1

233
G1482

Oryza sativa

ORYSA-22JAN02-
1022




CLUSTER99743_1

233
G1482

Oryza sativa

OSC101266.C1.p1.fg
1023

233
G1482

Oryza sativa

OSC15654.C1.p3.fg
1024

233
G1482

Zea mays

15631093
1025

233
G1482

Zea mays

ZEAMA-08NOV01-
1026




CLUSTER35072_1

233
G1482

Zea mays

ZEAMA-08NOV01-
1027




CLUSTER35072_2

233
G1482

Zea mays

ZEAMA-08NOV01-
1028




CLUSTER366705_1

233
G1482

Zea mays

ZEAMA-08NOV01-
1029




CLUSTER439033_1

233
G1482

Zea mays

ZEAMA-08NOV01-
1030




CLUSTER439033_2

233
G1482

Oryza sativa

Os_S60490
1592

233
G1482

Medicago

Mtr_S10820905
1703




truncatula


233
G1482

Zea mays

Zm_S11432778
1802

233
G1482

Triticum aestivum

Ta_S288030
1879

233
G1482

Lycopersicon

SGN-UNIGENE-47593
2032




esculentum


234
G1482

Solanum tuberosum

BM406201

1.00E−60

234
G1482

Medicago

CB894280

2.00E−57




truncatula


234
G1482

Robinia

BI678186

1.00E−52




pseudoacacia


234
G1482

Glycine max

BM954087

6.00E−52

234
G1482

Lotus japonicus

BI420251

1.00E−48

234
G1482

Zinnia elegans

AU288043

2.00E−45

234
G1482

Populus tremula

BU892726

2.00E−45

234
G1482

Lycopersicon

BM409788

2.00E−44




esculentum


234
G1482

Oryza sativa

AK071507

1.00E−43



( japonica cultivar-



group)

234
G1482

Oryza sativa

AB001884

5.00E−43

234
G1482

Oryza sativa

gi3618312

1.90E−45

234
G1482

Oryza sativa

gi32488104

2.00E−38



( japonica cultivar-



group)

234
G2482

Brassica nigra

gi11037311

4.90E−18

234
G1482

Raphanus sativus

gi3341723

8.00E−17

234
G1482

Brassica napus

gi30984027

2.70E−15

234
G1482

Malus x domestica

gi4091806

7.40E−15

234
G1482

Ipomoea nil

gi10946337

2.00E−14

234
G1482

Hordeum vulgare

gi21667485

2.90E−13

234
G1482

Hordeum vulgare

gi21655154

1.50E−11



subsp. vulgare

234
G1482

Pinus radiata

gi4557093

3.10E−10

236
G1483

Brassica oleracea

BZ463417

3.00E−57

236
G1483

Sorghum bicolor

CD212257

6.00E−34

236
G1483

Glycine max

CA802403

1.00E−33

236
G1483

Zinnia elegans

AU293301

3.00E−33

236
G1483

Oryza sativa

AB001886

4.00E−33

236
G1483

Oryza sativa

CB658637

4.00E−33



( japonica cultivar-



group)

236
G1483

Lycopersicon

BI935213

4.00E−32




esculentum


236
G1483

Populus tremula x

BU864618

6.00E−32




Populus tremuloides


236
G1483

Beta vulgaris

BQ589815

7.00E−32

236
G1483

Ipomoea nil

BJ559082

2.00E−31

236
G1483

Oryza sativa

gi3618316

7.20E−34

236
G1483

Oryza sativa

gi32488104

6.00E−30



( japonica cultivar-



group)

236
G1483

Brassica nigra

gi22854916

1.20E−12

236
G1483

Malus x domestica

gi4091806

4.70E−12

236
G1483

Brassica napus

gi2303681

7.90E−12

236
G1483

Raphanus sativus

gi3341723

1.40E−11

236
G1483

Hordeum vulgare

gi21667475

2.90E−11

236
G1483

Ipomoea nil

gi10946337

3.50E−09

236
G1483

Hordeum vulgare

gi21655160

1.90E−08



subsp. vulgare

236
G1483

Pinus radiata

gi4557093

4.30E−07

238
G1493

Medicago

CB891281

9.00E−98




truncatula


238
G1493

Zea mays

AB060130

5.00E−95

238
G1493

Brassica napus

CD825309

7.00E−84

238
G1493

Vitis vinifera

CD800109

9.00E−84

238
G1493

Oryza sativa

AK100530

7.00E−81



( japonica cultivar-



group)

238
G1493
Oryza sativa ( indica
CB630542

3.00E−77



cultivar-group)

238
G1493

Brassica oleracea

BH687265

2.00E−74

238
G1493

Glycine max

AW596288

4.00E−70

238
G1493

Poncirus trifoliata

CD574729

6.00E−69

238
G1493

Lactuca sativa

BQ858556

1.00E−66

238
G1493

Zea mays

gi13661174

1.00E−84

238
G1493

Oryza sativa

gi24308616

9.20E−82



( japonica cultivar-



group)

238
G1493
Oryza sativa ( indica
gi31338860

2.20E−42



cultivar-group)

238
G1493

Oryza glaberrima

gi31338862

2.20E−42

238
G1493

Oryza sativa

gi15289981

9.60E−19

238
G1493

Solanum

gi32470629

1.00E−10




bulbocastanum


238
G1493

Chlamydomonas

gi5916207

1.20E−09




reinhardtii


238
G1493

Mesembryanthemum

gi6942190

8.00E−09




crystallinum


238
G1493

Nicotiana tabacum

gi4519671

2.50E−08

238
G1493

Dianthus

gi13173408

1.40E−07




caryophyllus


240
G1507

Glycine max

BE440901

3.00E−61

240
G1507

Triticum aestivum

BQ295376

6.00E−56

240
G1507

Triticum

BF199732

1.00E−54




monococcum


240
G1507

Ipomoea nil

BJ569796

4.00E−54

240
G1507

Oryza sativa

AK068931

3.00E−53



( japonica cultivar-



group)

240
G1507

Zea mays

AY103800

6.00E−53

240
G1507

Lactuca sativa

BQ987329

2.00E−51

240
G1507

Beta vulgaris

BQ591642

4.00E−51

240
G1507

Hordeum vulgare

BU993000

1.00E−49

240
G1507

Medicago

CA991109

7.00E−49




truncatula


240
G1507

Oryza sativa

gi13174240

6.30E−51

240
G1507

Oryza sativa

gi24960749

3.50E−07



( japonica cultivar-



group)

240
G1507

Hordeum vulgare

gi21655156

7.50E−07



subsp. vulgare

240
G1507

Brassica rapa

gi28193631

1.20E−06



subsp. pekinensis

240
G1507

Raphanus sativus

gi3341723

2.10E−06

240
G1507

Brassica nigra

gi11037313

7.30E−06

240
G1507

Brassica napus

gi30984027

9.90E−06

240
G1507

Hordeum vulgare

gi21667485

9.60E−05

240
G1507

Malus x domestica

gi4091806

0.00013

240
G1507

Ipomoea nil

gi10946337

0.00068

241
G1510

Oryza sativa

ORYSA-22JAN02-
1031




CLUSTER159728_1

241
G1510

Oryza sativa

OSC101036.C1.p2.fg
1032

241
G1510

Glycine max

Gma_S5061040
1662

241
G1510

Triticum aestivum

Ta_S206702
1880

241
G1510

Lycopersicon

Les_S5271097
1932




esculentum


241
G1510

Lycopersicon

SGN-UNIGENE-56179
2033




esculentum


242
G1510

Brassica oleracea

BZ493938

8.00E−58

242
G1510

Brassica napus

CB686317

3.00E−31

242
G1510

Vitis vinifera

BM437179

5.00E−23

242
G1510

Glycine max

BF425622

5.00E−23

242
G1510

Oryza sativa

AK099607

7.00E−23



( japonica cultivar-



group)

242
G1510

Sorghum bicolor

CD213245

9.00E−20

242
G1510

Medicago

BQ165696

2.00E−18




truncatula


242
G1510

Populus tremula x

BU863159

5.00E−18




Populus tremuloides


242
G1510

Triticum aestivum

AL816777

4.00E−17

242
G1510

Oryza sativa

AC087597

3.00E−15

242
G1510

Oryza sativa

gi28372691

7.00E−19



( japonica cultivar-



group)

242
G1510

Oryza sativa

gi14165317

5.10E−10

242
G1510

Nicotiana tabacum

gi12711287

3.70E−07

242
G1510

Nicotiana

gi1076609

4.20E−05




plumbaginifolia


242
G1510
Fagopyrum sp.
gi31088153

0.013



C97107

242
G1510

Fagopyrum

gi31088139

0.016




rubifolium


242
G1510

Fagopyrum

gi31088119

0.032




gracilipes


242
G1510
Fagopyrum sp.
gi31088151

0.032



C97106

242
G1510

Fagopyrum

gi31088129

0.032




capillatum


242
G1510

Fagopyrum

gi31088131

0.04




callianthum


244
G1535

Gossypium

AF530914

1.0e−999




hirsutum


244
G1535

Oryza sativa

AB101646

1.0e−999



( japonica cultivar-



group)

244
G1535

Oryza sativa

AX699728

1.0e−999

244
G1535

Zea mays

ZMA250986

1.0e−999

244
G1535

Picea abies

AF172931

1.00E−147

244
G1535
Phalaenopsis sp.
PSU34743

1.00E−147



SM9108

244
G1535
Oryza sativa ( indica
AAAA01007245

1.00E−123



cultivar-group)

244
G1535

Malus domestica

AF067961

1.00E−110

244
G1535

Sorghum bicolor

AF466200

1.00E−106

244
G1535

Helianthus annuus

HNNHAHR

2.00E−97

244
G1535

Gossypium

gi22475197

1.50E−186




hirsutum


244
G1535

Oryza sativa

gi18266646

9.30E−174

244
G1535

Oryza sativa

gi31339099

9.30E−174



( japonica cultivar-



group)

244
G1535
Phalaenopsis sp.
gi1173622

1.10E−146



SM9108

244
G1535
Phalaenopsis sp.
gi2147484

1.10E−146

244
G1535

Picea abies

gi12002853

9.90E−146

244
G1535

Sorghum bicolor

gi18481701

1.60E−141

244
G1535

Zea mays

gi5531484

6.50E−134

244
G1535

Malus x domestica

gi3925363

3.10E−104

244
G1535

Helianthus annuus

gi1208940

1.40E−97

246
G1538

Brassica oleracea

BH555867

3.00E−57

246
G1538

Lycopersicon

AI897201

1.00E−37




esculentum


246
G1538

Solanum tuberosum

BQ506359

3.00E−36

246
G1538

Populus

BI138094

3.00E−36



balsmaifera subsp.




trichocarpa


246
G1538

Glycine max

BE660082

8.00E−36

246
G1538

Brassica napus

CD818428

2.00E−35

246
G1538

Medicago

BE204081

1.00E−34




truncatula


246
G1538

Helianthus

CF087358

1.00E−33




argophyllus


246
G1538

Gossypium

BG447432

3.00E−31




arboreum


246
G1538

Beta vulgaris

BQ584396

4.00E−30

246
G1538

Oryza sativa

gi33146844

2.10E−27



( japonica cultivar-



group)

246
G1538

Lycopersicon

gi1161575

1.10E−21




esculentum


246
G1538

Craterostigma

gi18034441

2.10E−20




plantagineum


246
G1538

Ceratopteris

gi3868841

2.70E−20



richardii

246
G1538

Oryza sativa

gi5006853

3.40E−20

246
G1538

Daucus carota

gi4433048

2.40E−19

246
G1538

Nicotiana tabacum

gi22651698

3.00E−19

246
G1538

Brassica rapa

gi8133126

3.90E−19



subsp. pekinensis

246
G1538

Phaseolus vulgaris

gi15148916

5.00E−19

246
G1538
Physcomitrella
gi21623495

1.30E−18




patens


247
G1539

Glycine max

BE800562.1
1033

247
G1539

Glycine max

uC-gmflminsoy098c11b1
1034

248
G1539

Brassica oleracea

BH725803

3.00E−46

248
G1539

Lactuca sativa

BU006325

1.00E−38

248
G1539

Ipomoea nil

BJ555660

4.00E−35

248
G1539

Medicago

AC137078

9.00E−32




truncatula


248
G1539

Glycine max

BE800562

1.00E−25

248
G1539

Vigna radiata

AF322401

2.00E−25

248
G1539
Oryza sativa ( indica
AAAA01009392

3.00E−24



cultivar-group)

248
G1539

Populus tremula x

BI128104

5.00E−24




Populus tremuloides


248
G1539

Lycopersicon

BG134747

5.00E−24




esculentum


248
G1539

Zea mays

CC337134

2.00E−23

248
G1539

Lycopersicon

gi28070968

3.30E−31




esculentum


248
G1539

Petunia x hybrida

gi22087128

7.10E−27

248
G1539

Oryza sativa

gi21740884

1.30E−25



( japonica cultivar-



group)

248
G1539

Oryza sativa

gi10241438

4.20E−25

248
G1539

Populus tremula x

gi3955021

4.50E−12




Populus tremuloides


248
G1539

Narcissus

gi18419580

0.00089




pseudonarcissus


248
G1539

Zinnia elegans

gi18076738

0.0053

248
G1539

Ceratopteris

gi3868829

0.074




richardii


248
G1539

Juniperus rigida

gi9280017

0.49

248
G1539

Physcomitrella

gi7209912

0.53




patens


250
G1549

Brassica napus

GD842307

2.00E−30

250
G1549

Medicago

AC139525

2.00E−29




truncatula


250
G1549

Brassica oleracea

BZ482689

5.00E−28

250
G1549

Glycine max

BI787228

1.00E−21

250
G1549

Physcomitrella

AB028079

2.00E−21




patens


250
G1549

Ipomoea batatas

BM878740

5.00E−21

250
G1549 Populus
BU881786

7.00E−21



balsamifera subsp.




trichocarpa


250
G1549

Lycopersicon

AI488741

9.00E−21




esculentum


250
G1549

Oryza sativa

AX654801

9.00E−21

250
G1549

Oryza sativa

AK105484

1.00E−20



( japonica cultivar-



group)

250
G1549

Physcomitrella

gi7415628

7.60E−23




patens


250
G1549

Lycopersicon

gi1161575

8.70E−22




esculentum


250
G1549

Daucus carota

gi1435021

8.70E−22

250
G1549

Oryza sativa

gi5006853

8.70E−22

250
G1549

Ceratopteris

gi3868839

2.30E−21




richardii


250
G1549

Glycine max

gi6091551

6.10E−21

250
G1549

Brassica rapa

gi8133126

1.00E−20



subsp. pekinensis

250
G1549

Nicotiana tabacum

gi22651698

1.30E−20

250
G1549

Phaseolus vulgaris

ggi15148916

1.60E−20

250
G1549

Helianthus annuus

gi349379

4.30E−20

252
G1554

Brassica oleracea

BZ436074

1.00E−70

252
G1554

Oryza sativa

AK109808

5.00E−68



( japonica cultivar-



group)

252
G1554

Lycopersicon

AW398166

4.00E−38




pennellii


252
G1554
Oryza sativa ( indica
AAAA01012151

5.00E−34



cultivar-group)

252
G1554

Lycopersicon

BE450553

1.00E−33




esculentum


252
G1554

Solanum tuberosum

BM111266

2.00E−33

252
G1554

Oryza sativa

AP002523

6.00E−33

252
G1554

Populus tremuloides

CA926221

1.00E−31

252
G1554

Medicago

CF068634

2.00E−31




truncatula


252
G1554

Hordeum vulgare

BU988945

8.00E−31



subsp. vulgare

252
G1554

Oryza sativa

gi11034542

4.00E−72

252
G1554

Oryza sativa

gi33146555

5.70E−44



( japonica cultivar-



group)

252
G1554

Nicotiana tabacum

gi4519671

6.20E−13

252
G1554

Zea mays

gi14189890

3.00E−12

252
G1554

Mesembryanthemum

gi6942190

7.70E−11




crystallinum


252
G1554
Chlamydomonas
gi5916207

7.80E−11




reinhardtii


252
G1554

Oryza glaberrima

gi31338862

6.80E−09

252
G1554

Solanum

gi32470629

8.30E−09




bulbocastanum


252
G1554
Oryza sativa ( indica
gi31338860

4.20E−08



cultivar-group)

252
G1554

Nicotiana alata

gi1087017

0.0056

254
G1556

Oryza sativa

AU172823

3.00E−18

254
G1556

Medicago

BE319599

3.00E−18




truncatula


254
G1556

Gossypium

AI730937

4.00E−18




hirsutum


254
G1556

Oryza sativa

AK107297

4.00E−18



( japonica cultivar-



group)

254
G1556

Solanum tuberosum

BG597254

1.00E−17

254
G1556

Lycopersicon

AW219675

1.00E−17




esculentum


254
G1556

Beta vulgaris

BQ585483

4.00E−17

254
G1556

Sorghum bicolor

BG240038

8.00E−17

254
G1556

Hordeum vulgare

BQ465062

1.00E−16

254
G1556

Triticum aestivum

CD913537

1.00E−16

254
G1556

Oryza sativa

gi21741799

2.70E−18



( japonica cultivar-



group)

254
G1556

Solanum

gi32470629

2.30E−17




bulbocastanum


254
G1556

Mesembryanthemum

gi6942190

2.80E−15




crystallinum


254
G1556

Nicotiana tabacum

gi4519671

4.70E−13

254
G1556

Chlamydomonas

gi5916207

2.50E−12




reinhardtii


254
G1556

Zea mays

gi13940496

1.20E−09

254
G1556

Oryza sativa

gi13940500

2.20E−09

254
G1556
Oryza sativa ( indica
gi31338860

1.10E−06



cultivar-group)

254
G1556

Oryza glaberrima

gi31338862

1.10E−06

254
G1556

Lycopersicon

gi22900937

0.96




esculentum


255
G1557

Zea mays

ZEAMA-08NOV01-
1035




CLUSTER70177_1

256
G1557

Gossypium

AI730937

2.00E−24




hirsutum


256
G1557

Beta vulgaris

BQ585483

2.00E−24

256
G1557

Solanum tuberosum

BG597254

3.00E−24

256
G1557
Mesembryanthemum
BE036811

1.00E−23



crystallinum

256
G1557

Medicago

BG646240

1.00E−23




truncatula


256
G1557

Lycopersicon

BG643313

3.00E−23




esculentum


256
G1557

Oryza sativa

AK107297

4.00E−22



( japonica cultivar-



group)

256
G1557

Populus

BU872452

8.00E−22



balsamifera subsp.




trichocarpa


256
G1557

Oryza sativa

AU172823

1.00E−21

256
G1557

Hordeum vulgare

BQ465062

7.00E−21

256
G1557

Oryza sativa

gi21741799

2.90E−23



( japonica cultivar-



group)

256
G1557

Nicotiana tabacum

gi4519671

3.60E−16

256
G1557

Solanum

gi32470629

1.20E−-15




bulbocastanum


256
G1557

Mesembryanthemum

gi6942190

3.60E−15




crystallinum


256
G1557

Chlamydomonas

gi5916207

3.50E−13




reinhardtii


256
G1557

Oryza sativa

gi11034542

3.20E−09

256
G1557
Oryza sativa ( indica
gi31338860

1.80E−07



cultivar-group)

256
G1557

Oryza glaberrima

gi31338862

1.80E−07

256
G1557

Zea mays

gi13661174

1.80E−07

256
G1557

Triticum aestivum

gi100791

0.99

258
G1585

Brassica oleracea

BZ441174

6.00E−57

258
G1585

Ipomoea nil

BJ559013

3.00E−37

258
G1585
Oryza sativa ( indica
AAAA010099O3

2.00E−29



cultivar-group)

258
G1585

Oryza sativa

AP003760

2.00E−29

258
G1585

Zea mays

AQ844430

5.00E−29

258
G1585

Oryza sativa

AC121360

9.00E−29



( japonica cultivar-



group)

258
G1585

Pinus taeda

AW981538

2.00E−24

258
G1585

Hordeum vulgare

BQ467157

3.00E−18



subsp. vulgare

258
G1585

Brassica napus

CD827898

4.00E−18

258
G1585

Triticum aestivum

CD923002

5.00E−18

258
G1585

Oryza sativa

gi20161583

1.50E−47



( japonica cultivar-



group)

258
G1585

Petunia x hybrida

gi22087128

1.50E−13

258
G1585

Populus tremula x

gi3955019

2.70E−12




Populus tremuloides


258
G1585

Lycopersicon

gi28070968

3.90E−12




esculentum


258
G1585

Oryza sativa

gi10241438

3.10E−11

258
G1585

Narcissus

gi18419580

3.00E−05




pseudonarcissus


258
G1585

Prunus armeniaca

gi5031277

0.027

258
G1585

Daucus carota

gi1076569

0.53

258
G1585

Picea abies

gi19070143

0.63

258
G1585 Zea mays
gi8920423

0.71

260
G1591

Brassica oleracea

BZ084919

2.00E−35

260
G1591

Oryza sativa

CNS09S4R

3.00E−35



( japonica cultivar-



group)

260
G1591
Oryza sativa ( indica
AAAA01009392

3.00E−35



cultivar-group)

260
G1591

Zea mays

CG337119

1.00E−33

260
G1591

Hordeum vulgare

BU998450

2.00E−31



subsp. vulgare

260
G1591

Oryza sativa

AC078977

1.00E−29

260
G1591

Ipomoea nil

BJ553325

1.00E−21

260
G1591

Medicago

AC137078

1.00E−21




truncatula


260
G1591

Lactuca sativa

BU006325

2.00E−21

260
G1591

Lycopersicon

BI204369

3.00E−20




esculentum


260
G1591

Oryza sativa

gi8099120

7.50E−31

260
G1591

Petunia x hybrida

gi22087128

4.10E−23

260
G1591

Lycopersicon

gi28070968

8.40E−23




esculentum


260
G1591

Oryza sativa

gi18461215

2.80E−22



( japonica cultivar-



group)

260
G1591

Populus tremula x

gi3955019

3.00E−12




Populus tremuloides


260
G1591

Chlamydomonas

gi16209575

0.00053




reinhardtii


260
G1591

Gossypium

gi5731257

0.0021




hirsutum


260
G1591

Narcissus

gi18419580

0.0022




pseudonarcissus


260
G1591

Dicentra eximia

gi3170468

0.0027

260
G1591

Zinnia elegans

gi18076738

0.012

262
G1593

Solanum tuberosum

AF406697

4.00E−67

262
G1593

Lycopersicon

AF375966

4.00E−67




esculentum


262
G1593

Medicago

AW688195

1.00E−57




truncatula


262
G1593

Oryza sativa

AK070465

4.00E−57



( japonica cultivar-



group)

262
G1593

Citrus sinensis

CB292855

1.00E−56

262
G1593

Glycine max

BQ629874

1.00E−56

262
G1593

Prunus persica

BU043836

6.00E−55

262
G1593

Vitis vinfera

CB343619

3.00E−54

262
G1593

Mesembryanthemum

CA839352

6.00E−54




crystallinum


262
G1593

Malus x domestica

AF053769

1.00E−49

262
G1593

Solanum tuberosum

gi22652115

9.50E−68

262
G1593

Lycopersicon

gi31323447

2.20E−64




esculentum


262
G1593

Malus x domestica

gi7239157

3.00E−52

262
G1593

Gnetum gnemon

gi31746344

5.40E−52

262
G1593

Oryza sativa

gi20219036

6.80E−51



( japonica cultivar-



group)

262
G1593

Hordeum vulgare

gi13752407

1.80E−48

262
G1593

Oryza sativa

gi15408891

3.20E−47

262
G1593
Oryza sativa ( indica
gi19352101

3.20E−47



cultivar-group)

262
G1593

Zea mays

gi19743685

4.90E−37

262
G1593

Dendrobium grex

gi3929314

4.30E−12



Madame Thong-In

263
G1660

Glycine max

GLYMA-28NOV01-
1036




CLUSTER30666_1

263
G1660

Glycine max

uC-
1037




gmflLIB3275P059b07b1

263
G1660

Oryza sativa

ORYSA-22JAN02-
1038




CLUSTER6548_1

263
G1660

Oryza sativa

ORYSA-22JAN02-
1039




CLUSTER93242_1

263
G1660

Oryza sativa

OSC100113.C1.p9.fg
1040

263
G1660

Oryza sativa

OSC101572.C1.p8.fg
1041

263
G1660

Oryza sativa

OSC34319.C1.p4.fg
1042

263
G1660

Zea mays

700167489_FLI
1043

263
G1660

Zea mays

LIB3279-010-H4_FLI
1044

263
G1660

Zea mays

LIB4767-001-R1-M1-D1
1045

263
G1660

Zea mays

ZEAMA-08NOV01-
1046




CLUSTER43109_1

263
G1660

Zea mays

ZEAMA-08NOV01-
1047




CLUSTER64649_1

263
G1660

Oryza sativa

Os_S94670
1593

263
G1660

Zea mays

Zm_S11454293
1803

263
G1660

Zea mays

Zm_S11520265
1804

263
G1660

Triticum aestivum

Ta_S142271
1881

263
G1660

Lycopersicon

SGN-UNIGENE-
2034




esculentum

SINGLET-35095

263
G1660

Lycopersicon

SGN-UNIGENE-
2035




esculentum

SINGLET-53090

264
G1660

Oryza sativa

AK102604

1.00E−109



( japonica cultivar-



group)

264
G1660

Brassica oleracea

BZ431607

1.00E−108

264
G1660

Brassica napus

CD818917

2.00E−95

264
G1660

Oryza sativa

BE040229

2.00E−62

264
G1660

Ipomoea nil

BJ576287

1.00E−54

264
G1660

Lycopersicon

AW443990

7.00E−54




esculentum


264
G1660
Oryza sativa ( indica
AAAA01001098

2.00E−52



cultivar-group)

264
G1660

Zea mays

GB886289

3.00E−50

264
G1660

Hordeum vulgare

BM377843

3.00E−50

264
G1660

Triticum aestivum

BJ238027

6.00E−47

264
G1660

Oryza sativa

gi27452912

7.70E−62



( japonica cultivar-



group)

264
G1660

Zea mays

gi23928441

3.30E−22

264
G1660

Solanum tuberosum

gi1881585

1.60E−17

264
G1660

Lycopersicon

gi4731573

1.20E−16




esculentum


264
G1660

Nicotiana tabacum

gi8096269

0.0017

264
G1660

Cucurbita maxima

gi17221648

0.002

264
G1660
Cicer arietinum
gi7208779

0.0026

264
G1660

Oryza sativa

gi11875196

0.006

264
G1660

Plastid Oenothera

gi13276714

0.0063



elata subsp. hookeri

264
G1660

Oenothera elata

gi23822375

0.0063



subsp. hookeri

266
G1718

Brassica rapa

BG543482

2.00E−77



subsp. pekinensis

266
G1718

Brassica oleracea

BH533325

2.00E−65

266
G1718

Citrus sinensis

CB292402

2.00E−47

266
G1718

Vitis vinifera

CA812994

3.00E−47

266
G1718

Populus tremula

BU889204

4.00E−47

266
G1718

Populus tremula x

BU835838

3.00E−46




Populus tremuloides


266
G1718

Populus tremuloides

CA925038

8.00E−46

266
G1718

Euphorbia esula

BE056347

1.00E−44

266
G1718

Medicago

BG452491

4.00E−44




truncatula


266
G1718

Populus

BU869861

7.00E−44



balsamifera subsp.




trichocarpa


266
G1718

Oryza sativa

gi32129334

1.60E−21



( japonica cultivar-



group)

266
G1718

Oryza sativa

gi6539567

4.30E−20

266
G1718

Cicer arietinum

gi4651204

6.50E−17

266
G1718

Tulipa gesneriana

gi23386073

3.30E−14

266
G1718

Cucumis melo

gi17016985

3.40E−11

266
G1718

Hordeum vulgare

gi20152976

1.40E−08



subsp. vulgare

266
G1718

Thellungiella

gi20340241

1.60E−08




halophila


266
G1718
Oryza sativa ( indica
gi29164825

2.40E−08



cultivar-group)

266
G1718

Medicago sativa

gi23451086

1.30E−07

266
G1718

Lotus japonicus

gi1086225

2.30E−07

267
G1730

Zea mays

LIB5074-010-R1-XP1-
1048




A11

268
G1730

Brassica oleracea

BZ472679

6.00E−67

268
G1730

Medicago

AC126787

1.00E−27




truncatula


268
G1730

Brassica napus

CD814199

4.00E−27

268
G1730

Zea mays

BZ715596

4.00E−21

268
G1730

Oryza sativa

AK108491

5.00E−21



( japonica cultivar-



group)

268
G1730
Oryza sativa ( indica
AAAA01009602

7.00E−21



cultivar-group)

268
G1730

Oryza sativa

AX653298

1.00E−18

268
G1730

Cucumis melo

AF499727

2.00E−18

268
G1730

Solanum tuberosum

B0593372

5.00E−18

268
G1730

Lycopersicon

AW032769

2.00E−17




esculentum


268
G1730

Cucumis melo

gi28558782

6.70E−23

268
G1730

Oryza sativa

gi12643047

1.90E−19

268
G1730

Oryza sativa

gi31433649

1.90E−19



( japonica cultivar-



group)

268
G1730

Nicotiana tabacum

gi12003386

5.10E−17

268
G1730

Zea mays

gi21645888

1.40E−16

268
G1730

Medicago sativa

gi23451086

1.30E−14

268
G1730

Hordeum vulgare

gi20152976

5.70E−14



subsp. vulgare

268
G1730

Hordeum vulgare

gi2894379

1.10E−09

268
G1730
Oryza sativa ( indica
gi29164825

4.10E−09



cultivar-group)

268
G1730

Thellungiella

gi20340241

1.10E−08




halophila


270
G1743

Brassica oleracea

BH985728

1.00E−89

270
G1743

Populus tremula

BU891914

3.00E−61

270
G1743

Populus tremula x

BU885427

3.00E−61




Populus tremuloides


270
G1743

Lycopersicon

AW034559

1.00E−56




esculentum


270
G1743

Ipomoea nil

BJ561648

1.00E−56

270
G1743

Gossypium

GA993166

1.00E−55




hirsutum


270
G1743

Populus

CA825344

2.00E−54



balsamifera subsp.




trichocarpa x





Populus deltoides


270
G1743

Vitis vinifera

GB918599

3.00E−53

270
G1743

Lactuca sativa

BQ849490

4.00E−53

270
G1743

Capsicum annuum

CA847343

2.00E−52

270
G1743

Oryza sativa

gi32488512

1.80E−35



( japonica cultivar-



group)

270
G1743

Oryza sativa

gi6069662

1.30E−18

270
G1743

Thellungiella

gi20340241

1.70E−16




halophila


270
G1743

Zea mays

gi18092342

6.30E−12

270
G1743

Medicago sativa

gi23451086

1.20E−10

270
G1743

Hordeum vulgare

gi20152976

1.50E−10



subsp. vulgare

270
G1743

Nicotiana tabacum

gi12003386

2.20E−09

270
G1743

Cucumis melo

gi28558782

2.20E−09

270
G1743

Hordeum vulgare

gi2894379

2.70E−08

270
G1743

Cicer arietinum

gi4651204

8.50E−08

271
G1753

Glycine max

GLYMA-28NOV01-
1049




CLUSTER91438_1

271
G1753

Oryza sativa

OSC101736.C1.p16.fg
1050

272
G1753

Brassica oleracea

BZ063578

4.00E−78

272
G1753

Lycopersicon

AW030833

4.00E−35




esculentum


272
G1753
Oryza sativa ( indica
AAAA01009545

9.00E−35



cultivar-group)

272
G1753

Oryza sativa

AP005775

1.00E−34



( japonica cultivar-



group)

272
G1753
Poncirus trifoliata
CD576150

1.00E−33

272
G1753

Oryza sativa

AX653721

4.00E−33

272
G1753

Helianthus annuus

BQ976989

5.00E−33

272
G1753

Zea mays

BZ737969

1.00E−32

272
G1753

Nicotiana tabacum

AF211531

4.00E−32

272
G1753

Vitis vinifera

CB035846

1.00E−31

272
G1753

Oryza sativa

gi21742362

1.00E−34



( japonica cultivar-



group)

272
G1753

Oryza sativa

gi14140155

2.70E−34

272
G1753

Nicotiana tabacum

gi12003384

1.90E−33

272
G1753

Brassica napus

gi20303011

5.10E−33

272
01753

Lycopersicon

gi18535580

1.60E−31




esculentum


272
G1753

Prunus avium

gi23495458

1.40E−30

272
G1753

Hordeum vulgare

gi19071243

1.40E−28

272
G1753

Zea mays

gi21908034

1.70E−25

272
G1753

Hordeum vulgare

gi20152903

5.50E−20



subsp. vulgare

272
G1753

Gossypium

gi32481079

1.30E−18




hirsutum


274
G1772

Oryza sativa

AK073139

1.0e−999



( japonica cultivar-



group)

274
G1772

Thellungiella

BM985639

1.00E−134




halophila


274
G1772

Lactuca sativa

BQ996439

1.00E−119

274
G1772

Vitis vinifera

CD008605

6.00E−99

274
G1772

Brassica oleracea

BH998711

6.00E−93

274
G1772

Glycine max

BM887188

1.00E−90

274
G1772

Helianthus annuus

BU026535

9.00E−89

274
G1772

Zea mays

BM661323

1.00E−84

274
G1772
Oryza sativa ( indica
AAAA01003274

4.00E−73



cultivar-group)

274
G1772

Oryza sativa

AC103891

5.00E−73

274
G1772

Oryza sativa

gi20330766

1.30E−191



( japonica cultivar-



group)

274
G1772

Nicotiana

gi1666171

2.20E−34




plumbaginifolia


274
G1772

Brassica rapa

gi27804453

0.16



subsp. pekinensis

274
G1772

Calycanthus

gi8163958

0.64




floridus


274
G1772

Picea glauca

gi1350524

0.66

274
G1772

Hordeum vulgare

gi23954355

0.84



subsp. vulgare

274
G1772

Adiantum capillus-

gi30266733

0.95




veneris


274
G1772

Macrotyloma

gi124034

0.98




axillare


274
G1772

Canavalia lineata

gi543526

1

274
G1772

Glycine max

gi2306979

1

275
G1779

Glycine max

GLYMA-28NOV01-
1051




CLUSTER185518_1

275
G1779

Glycine max

GLYMA-28NOV01-
1052




CLUSTER264928_1

275
G1779

Glycine max

GLYMA-28NOV01-
1053




CLUSTER76652_1

275
G1779

Oryza sativa

OSC21832.C1.p4.fg
1054

275
G1779

Zea mays

ZEAMA-08NOV01-
1055




CLUSTER78309_1

275
G1779

Lycopersicon

SGN-UNIGENE-
2036




esculentum

SINGLET-56681

276
G1779

Brassica oleracea

BH558232

3.00E−36

276
G1779

Vitis vinifera

BM437179

2.00E−26

276
G1779

Glycine max

BF425622

1.00E−24

276
G1779

Oryza sativa

AK099607

5.00E−21



( japonica cultivar-



group)

276
G1779

Sorghum bicolor

CD213245

3.00E−20

276
G1779

Medicago

BQ165696

2.00E−19




truncatula


276
G1779

Populus tremula x

BU863159

2.00E−18




Populus tremuloides


276
G1779

Brassica napus

CB686317

9.00E−18

276
G1779

Poncirus trifoliata

CD576018

3.00E−17

276
G1779

Triticum aestivum

AL816777

2.00E−16

276
G1779

Oryza sativa

gi28564714

1.20E−20



( japonica cultivar-



group)

276
G1779

Oryza sativa

gi5091599

2.80E−08

276
G1779

Nicotiana tabacum

gi12711287

2.90E−07

276
G1779

Nicotiana

gi1076609

3.50E−05




plumbaginifolia


276
G1779

Lycopersicon

gi1418988

0.36




esculentum


276
G1779

Eutrema wasabi

gi23200602

0.55

276
G1779

Amicia glandulosa

gi30313971

0.62

276
G1779

Ipomoea batatas

gi604324

0.8

276
G1779

Triticum aestivum

gi23451222

1

276
G1779
Gnetum gnemon
gi31746346

1

277
G1792

Oryza sativa

G3380
2124
5.00E−29

277
G1792

Oryza sativa

G3383
2128
3.00E−33

277
G1792

Oryza sativa

G3515
2209
7.00E−30

277
G1792

Zea mays

G3516
2211
2.00E−31

277
G1792

Zea mays

G3517
2213
9.00E−33

277
G1792

Glycine max

G3518
2215
9.00E−35

277
G1792

Glycine max

G3519
2217
3.00E−35

277
G1792

Glycine max

G3520
2219
3.00E−36

277
G1792

Glycine max

AW308784.1
685

277
G1792

Glycine max

BG790680.1
686

277
G1792

Glycine max

GLYMA-28NOV01-
687




CLUSTER602185_1

277
G1792

Glycine max

GLYMA-28NOV01-
688




CLUSTER91218_1

277
G1792

Glycine max

LIB5118-009-Q1-PF1-F2
689

277
G1792

Oryza sativa

OSC20174.C1.p2.fg
690

277
G1792

Zea mays

LIB4756-134-A1-K1-
691




G10

277
G1792

Glycine max

Gma_S5001644
1633

277
G1792

Zea mays

Zm_S11513768
1754

278
G1792

Lycopersicon

AI776626

7.00E−35




esculentum


278
G1792

Solanum tuberosum

BQ045702

1.00E−32

278
G1792

Glycine max

BM178875

9.00E−32

278
G1792

Medicago

BF649790

2.00E−31




truncatula


278
G1792

Eucalyptus grandis

CB967722

1.00E−30

278
G1792

Brassica oleracea

BZ020356

1.00E−30

278
G1792
Oryza sativa ( indica
AAAA01002491

4.00E−30



cultivar-group)

278
G1792

Oryza sativa

AE017099

4.00E−30



( japonica cultivar-



group)

278
G1792

Oryza sativa

AC025907

4.00E−30

278
G1792

Sorghum bicolor

BZ337899

4.00E−30

278
G1792

Oryza sativa

gi31432356

1.10E−30



( japonica cultivar-



group)

278
G1792

Lycopersicon

gi23452024

4.90E−26




esculentum


278
G1792

Nicotiana tabacum

gi1732406

2.60E−25

278
G1792

Oryza sativa

gi12597874

4.50E−25

278
G1792

Mesembryanthemum

gi32401273

9.40E−25




crystallinum


278
G1792

Catharanthus

gi8980313

2.20E−23




roseus


278
G1792

Nicotiana sylvestris

gi8809571

2.20E−23

278
G1792

Matricaria

gi17385636

1.40E−21




chamomilla


278
G1792

Glycine max

gi21304712

3.80E−21

278
G1792

Atriplex hortensis

gi8571476

1.30E−20

279
G1796

Glycine max

GLYMA-28NOV01-
1056




CLUSTER50695_1

279
G1796

Oryza sativa

Os_S43212
1594

279
G1796

Zea mays

Zm_S11435953
1805

279
G1796

Triticum aestivum

Ta_S369485
1882

280
G1796

Brassica oleracea

BH503101

2.00E−54

280
G1796

Medicago

AC119414

2.00E−35




truncatula


280
G1796

Oryza sativa

AP004079

2.00E−25

280
G1796
Oryza sativa ( indica
AAAA01000133

2.00E−25



cultivar-group)

280
G1796

Zea mays

CD437690

3.00E−24

280
G1796

Lactuca sativa

BQ987300

4.00E−24

280
G1796

Sorghum bicolor

CD228394

4.00E−24

280
G1796

Lupinus albus

CA411234

6.00E−24

280
G1796

Glycine max

CA800025

8.00E−24

280
G1796

Oryza sativa

AP005845

2.00E−23



( japonica cultivar-



group)

280
G1796

Oryza sativa

gi32487928

6.40E−26



( japonica cultivar-



group)

280
G1796

Zea mays

gi27802487

1.80E−24

280
G1796

Nicotiana tabacum

gi1208496

1.10E−23

280
G1796

Oryza sativa

gi10567106

2.20E−23

280
G1796

Stylosanthes hamata

gi4099914

3.70E−23

280
G1796

Nicotiana sylvestris

gi8809573

1.20E−22

280
G1796

Lycopersicon

gi30526297

2.60E−22




esculentum


280
G1796

Thellungiella

gi20340233

4.20E−22




halophila


280
G1796

Mesembryanthemum

gi32401273

7.80E−21




crystallinum


280
G1796

Solanum tuberosum

gi28268684

1.30E−20

282
G1797

Petunia x hybrida

AF335240

5.00E−52

282
G1797

Lycopersicon

AI486684

7.00E−49




esculentum


282
G1797

Eucalyptus grandis

AY263808

8.00E−47

282
G1797 Eucalyptus
AY273872

7.00E−46




occidentalis


282
G1797

Populus tremuloides

CA925124

8.00E−45

282
G1797

Brassica rapa

AY257541

5.00E−44



subsp. pekinensis

282
G1797

Sinapis alba

SAU25696

5.00E−44

282
G1797

Pimpinella

AF082531

5.00E−44




brachycarpa


282
G1797

Cardamine flexuosa

AY257542

2.00E−43

282
G1797

Nicotiana tabacum

NTTOB

5.00E−43

282
G1797

Petunia x hybrida

gi13384058

4.40E−50

282
G1797

Eucalyptus grandis

gi30575600

8.60E−47

282
G1797

Eucalyptus

gi30983946

6.00E−46




occidentalis


282
G1797

Brassica rapa

gi30171307

4.90E−44



subsp. pekinensis

282
G1797

Populus tremuloides

gi31295609

4.90E−44

282
G1797

Sinapis alba

gi1049022

1.60E−43

282
G1797

Pimpinella

gi3493647

1.60E−43




brachycarpa


282
G1797

Cardamine flexuosa

gi30171309

2.70E−43

282
G1797

Nicotiana tabacum

gi1076646

1.50E−42

282
G1797

Draba nemorosa

gi30171311

1.00E−41



var. hebecarpa

284
G1798

Petunia x hybrida

AF335240

5.00E−53

284
G1798

Lycopersicon

AI486684

3.00E−52




esculentum


284
G1798

Brassica rapa

AY257541

3.00E−48



subsp. pekinensis

284
G1798

Sinapis alba

SAU25696

3.00E−47

284
G1798

Cardamine flexuosa

AY257542

5.00E−47

284
G1798

Pimpinella

AF082531

5.00E−47




brachycarpa


284
G1798

Populus tremuloides

CA925124

1.00E−44

284
G1798

Eucalyptus grandis

AY263807

1.00E−43

284
G1798

Nicotiana tabacum

NTTOB

1.00E−43

284
G1798

Oryza sativa

AK104921

5.00E−43



( japonica cultivar-



group)

284
G1798

Petunia x hybrida

gi13384058

1.30E−52

284
G1798

Brassica rapa

gi30171307

4.60E−48



subsp. pekinensis

284
G1798

Sinapis alba

gi1049022

2.50E−47

284
G1798

Cardamine flexuosa

gi30171309

1.40E−46

284
G1798

Pimpinella

gi3493647

1.40E−46




brachycarpa


284
G1798

Populus tremuloides

gi31295609

2.30E−44

284
G1798

Oryza sativa

gi5295990

6.20E−44

284
G1798

Eucalyptus grandis

gi30575598

1.00E−43

284
G1798

Zea mays

gi12002139

1.30E−43

284
G1798

Nicotiana tabacum

gi1076646

5.60E−43

286
G1808

Brassica oleracea

BH950967

1.00E−85

286
G1808

Lycopersicon

BF051268

2.00E−30




esculentum


286
G1808

Populus

BU870843

2.00E−29



balsamifera subsp.




trichocarpa


286
G1808

Glycine max

BM269595

6.00E−21

286
G1808

Brassica napus

CD833815

3.00E−17

286
G1808

Gossypium

CA992680

6.00E−14




hirsutum


286
G1808

Zinnia elegans

AU294545

4.00E−12

286
G1808

Solanum tuberosum

BQ519273

9.00E−12

286
G1808

Medicago

CA918476

1.00E−11




truncatula


286
G1808

Phaseolus vulgaris

AF350505

2.00E−11

286
G1808

Phaseolus vulgaris

gi13430400

2.90E−14

286
G1808

Phaseolus

gi12829956

6.10E−14




acutifolius


286
G1808

Petroselinum

gi9650828

7.70E−14




crispum


286
G1808

Glycine max

gi22597162

1.30E−13

286
G1808

Nicotiana tabacum

gi16580130

1.10E−12

286
G1808

Capsicum chinense

gi24460973

1.80E−12

286
G1808

Zea mays

gi1060935

2.00E−11

286
G1808

Lycopersicon

gi5901747

8.10E−11




esculentum


286
G1808

Zea perennis

gi27652122

1.30E−10

286
G1808

Oryza sativa

gi14289165

1.40E−10

287
G1816

Oryza sativa

G3392
2131
2.00E−16

287
G1816

Oryza sativa

G3392
2133
2.00E−15

287
G1816

Zea mays

G3431
2147
1.00E−13

287
G1816

Zea mays

G3444
2157
1.00E−13

287
G1816

Glycine max

G3445
2159
5.00E−12

287
G1816

Glycine max

G3446
2161
5.00E−12

287
G1816

Glycine max

G3447
2163
5.00E−12

287
G1816

Glycine max

G3448
2165
1.00E−13

287
G1816

Glycine max

G3449
2167
3.00E−14

287
G1816

Glycine max

G3450
2168
3.00E−22

287
G1816

Glycine max

GLYMA-28NOV01-
1057




CLUSTER31802_1

287
G1816

Glycine max

GLYMA-28NOV01-
1058




CLUSTER586_102

287
G1816

Glycine max

GLYMA-28NOV01-
1059




CLUSTER586_116

287
G1816

Glycine max

GLYMA-28NOV01-
1060




CLUSTER8724_1

287
G1816

Glycine max

GLYMA-28NOV01-
1061




CLUSTER8724_2

287
G1816

Oryza sativa

ORYSA-22JAN02-
1062




CLUSTER30974_2

287
G1816

Oryza sativa

ORYSA-22JAN02-
1063




CLUSTER30974_3

287
G1816

Oryza sativa

OSC20053.C1.p5.fg
1064

287
G1816

Oryza sativa

OSC20055.C1.p5.fg
1065

287
G1816

Zea mays

ZEAMA-08NOV01-
1066




CLUSTER69699_1

287
G1816

Zea mays

ZEAMA-08NOV01-
1067




CLUSTER69699_2

287
G1816

Glycine max

Gma_S4901946
1663

287
G1816

Triticum aestivum

Ta_S45274
1883

288
G1816

Vitis vinifera

BM437313

8.00E−28

288
G1816

Populus

BU872107

2.00E−27



balsamifera subsp.




trichocarpa


288
G1816

Populus tremula x

BU831849

2.00E−27




Populus tremuloides


288
G1816

Vitis aestivalis

CB289238

7.00E−27

288
G1816

Glycine max

AI495284

7.00E−19

288
G1816

Brassica napus

CD843377

6.00E−15

288
G1816

Nuphar advena

CD473522

1.00E−14

288
G1816

Pinus pinaster

AL750151

3.00E−14

288
G1816

Lactuca sativa

BU015255

5.00E−14

288
G1816

Brassica oleracea

BH961028

8.00E−14

288
G1816

Gossypioides kirkii

gi23476295

4.90E−12

288
G1816

Gossypium

gi14269333

2.70E−11




raimondii


288
G1816

Gossypium

gi14269335

2.70E−11




herbaceum


288
G1816

Gossypium

gi14269337

2.70E−11




hirsutum


288
G1816

Solanum tuberosum

gi9954118

1.50E−10

288
G1816

Oryza sativa

gi2605619

2.40E−10

288
G1816

Cucumis sativus

gi20514371

3.10E−10

288
G1816
Zea mays subsp.
gi15042108

4.00E−10




parviglumis


288
G1816

Zea luxurians

gi15042124

4.00E−10

288
G1816

Anthurium

gi29824962

5.20E−10




andraeanum


290
G1823

Zea mays

AB060130

3.00E−81

290
G1823

Oryza sativa

AK065276

8.00E−74



( japonica cultivar-



group)

290
G1823
Oryza sativa ( indica
CB630542

5.00E−68



cultivar-group)

290
G1823

Lactuca sativa

BQ858556

3.00E−64

290
G1823

Brassica oleracea

BH485050

3.00E−58

290
G1823

Solanum tuberosum

BM407041

3.00E−56

290
G1823

Medicago

CB891281

3.00E−54




truncatula


290
G1823

Vitis vinifera

GD800109

3.00E−52

290
G1823

Sorghum bicolor

CD424269

1.00E−51

290
G1823

Stevia rebaudiana

BG523436

1.00E−47

290
G1823

Zea mays

gi15667625

3.10E−79

290
G1823

Oryza sativa

gi24308616

3.70E−78



( japonica cultivar-



group)

290
G1823
Oryza sativa ( indica
gi31338860

4.50E−41



cultivar-group)

290
G1823

Oryza glaberrima

gi31338862

4.50E−41

290
G1823

Oryza sativa

gi15289981

2.40E−19

290
G1823

Chlamydomonas

gi5916207

4.80E−11




reinhardtii


290
G1823

Nicotiana tabacum

gi4519671

1.40E−09

290
G1823

Solanum

gi32470629

6.40E−09




bulbocastanum


290
G1823

Mesembryanthemum

gi6942190

3.80E−08




crystallinum


290
G1823

Dianthus

gi13173408

6.50E−07




caryophyllus


292
G1825

Lycopersicon

BG643313

4.00E−28




esculentum


292
G1825

Medicago

BG646240

3.00E−27




truncatula


292
G1825

Oryza sativa

AK073606

6.00E−27



( japonica cultivar-



group)

292
G1825

Oryza sativa

AU172823

6.00E−27

292
G1825

Gossypium

AI730937

1.00E−25




hirsutum


292
G1825

Triticum aestivum

BJ288732

3.00E−25

292
G1825

Hordeum vulgare

BQ465062

1.00E−24

292
G1825

Lactuca sativa

BQ867305

2.00E−22

292
G1825

Beta vulgaris

BQ585483

3.00E−22

292
G1825

Solanum tuberosum

BQ507198

8.00E−22

292
G1825

Oryza sativa

gi21741799

8.70E−22



( japonica cultivar-



group)

292
G1825

Mesembryanthemum

gi6942190

2.50E−18




crystallinum


292
G1825

Nicotiana tabacum

gi4519671

1.10E−16

292
G1825

Solanum

gi32470629

2.20E−16




bulbocastanum


292
G1825

Chlamydomonas

gi5916207

4.80E−14




reinhardtii


292
G1825

Oryza sativa

gi15289981

1.10E−08

292
G1825

Zea mays

gi14189890

5.10E−08

292
G1825

Oryza glaberrima

gi31338862

1.00E−06

292
G1825
Oryza sativa ( indica
gi31338860

1.40E−06



cultivar-group)

292
G1825

Juglans nigra x

gi20068283

0.13




juglans regia


294
G1832

Brassica oleracea

BZ063396

4.00E−42

294
G1832

Lotus japonicus

AP004945

2.00E−29

294
G1832

Zea mays

BZ413336

2.00E−24

294
G1832

Petunia x hybrida

AB000455

1.00E−23

294
G1832

Oryza sativa

AP003988

8.00E−22



( japonica cultivar-



group)

294
G1832
Oryza sativa ( indica
AAAA01007143

7.00E−21



cultivar-group)

294
G1832

Vitis vinifera

CD010326

2.00E−17

294
G1832

Oryza sativa

OSJN00060

2.00E−16

294
G1832

Thellungiella

BM985806

7.00E−16




halophila


294
G1832

Glycine max

GMU68763

3.00E−15

294
G1832

Petunia x hybrida

gi1786142

1.30E−31

294
G1832

Brassica rapa

gi2058504

7.00E−20

294
G1832

Oryza sativa

gi21740840

2.10E−19



( japonica cultivar-



group)

294
G1832

Glycine max

gi1763063

7.50E−16

294
G1832

Medicago sativa

gi7228329

1.80E−14

294
G1832

Datisca glomerata

gi4666360

3.10E−13

294
G1832

Nicotiana tabacum

gi2981169

4.70E−12

294
G1832

Triticum aestivum

gi485814

7.00E−12

294
G1832

Oryza sativa

gi12698882

1.20E−11

294
G1832

Pisum sativum

gi2129892

4.00E−09

296
G1837

Brassica oleracea

BH675531

5.00E−59

296
G1837

Oryza sativa

AK100643

4.00E−34



( japonica cultivar-



group)

296
G1837

Populus tremuloides

CA925570

1.00E−30

296
G1837

Oryza sativa

AP003238

7.00E−30

296
G1837

Gossypium

AY125487

7.00E−27




hirsutum


296
G1837

Ipomoea batatas

CB329929

1.00E−26

296
G1837

Helianthus annuus

BU026443

6.00E−26

296
G1837

Lactuca sativa

BU007581

7.00E−26

296
G1837

Hordeum vulgare

CA030236

4.00E−25



subsp. vulgare

296
G1837

Hordeum vulgare

BQ470403

3.00E−24

296
G1837

Oryza sativa

gi20146230

2.20E−36



( japonica cultivar-



group)

296
G1837

Gossypium

gi22858664

2.40E−29




hirsutum


296
G1837

Oryza sativa

gi13124871

2.60E−29

296
G1837

Marsilea

gi22550110

1.30E−11




quadrifolia


296
G1837

Lycopersicon

gi15144510

0.00029




esculentum


296
G1837

Zea mays

gi18568274

0.013

296
G1837

Nicotiana tabacum

gi14423763

0.016

296
G1837

Hordeum vulgare

gi20152975

0.024



subsp. vulgare

296
G1837

Cucurbita maxima

gi17221648

0.07

296
G1837

Nicotiana alata

gi7649694

0.089

297
G1840

Triticum aestivum

Ta_S359268
1884

298
G1840

Brassica oleracea

BH560552

2.00E−47

298
G1840

Medicago

AW559374

2.00E−19




truncatula


298
G1840

Vitis vinifera

CB003361

3.00E−19

298
G1840

Vitis aestivalis

CB289747

9.00E−19

298
G1840

Glycine max

AW152963

1.00E−17

298
G1840

Zea mays

CC729672

3.00E−15

298
G1840
Oryza sativa ( indica
AAAA01012262

4.00E−15



cultivar-group)

298
G1840

Oryza sativa

AP004399

4.00E−15



( japonica cultivar-



group)

298
G1840

Hordeum vulgare

BU998389

4.00E−14



subsp. vulgare

298
G1840

Hordeum vulgare

BQ469024

9.00E−14

298
G1840

Oryza sativa

gi20160854

6.80E−14



( japonica cultivar-



group)

298
G1840

Zea mays

gi21908036

3.20E−13

298
G1840

Lycopersicon

gi18535580

2.40E−12




esculentum


298
G1840

Nicotiana tabacum

gi10798644

1.00E−11

298
G1840

Oryza sativa

gi14018047

1.40E−11

298
G1840

Solanum tuberosum

gi28268684

1.60E−11

298
G1840

Thellungiella

gi20340233

2.70E−11




halophila


298
G1840

Narcissus

gi18266198

4.40E−11




pseudonarcissus


298
G1840

Hordeum vulgare

gi27960760

8.20E−11

298
G1840

Fagus sylvatica

gi18496063

1.10E−10

300
G1846

Brassica oleracea

BH486983

1.00E−68

300
G1846

Vitis vinifera

CB971990

4.00E−48

300
G1846

Solanum tuberosum

BG593364

3.00E−45

300
G1846

Medicago

AC137546

5.00E−43




truncatula


300
G1846

Glycine max

BM271306

6.00E−43

300
G1846

Vitis aestivalis

CB288995

2.00E−42

300
G1846

Lycopersicon

AF500012

2.00E−39




esculentum


300
G1846

Beta vulgaris

BQ594833

2.00E−39

300
G1846

Lactuca sativa

BQ988768

3.00E−39

300
G1846

Oryza sativa

AX653721

3.00E−34

300
G1846

Lycopersicon

gi25992102

1.40E−39




esculentum


300
G1846

Oryza sativa

gi14140155

3.90E−35

300
G1846

Oryza sativa

gi21742358

9.30E−34



( japonica cultivar-



group)

300
G1846

Zea mays

gi21908034

1.60E−31

300
G1846

Brassica napus

gi17352283

1.70E−25

300
G1846

Nicotiana tabacum

gi12003382

2.80E−25

300
G1846

Prunus avium

gi23495460

3.70E−23

300
G1846

Hordeum vulgare

gi19071243

2.00E−22

300
G1846

Gossypium

gi32481079

2.30E−21




hirsutum


300
G1846
Fagus sylvatica
gi18496063

2.70E−19

302
G1850

Lotus japonicus

AP006148

6.00E−81

302
G1850

Medicago

BG646618

7.00E−76




truncatula


302
G1850

Oryza sativa

AK106525

2.00E−75



( japonica cultivar-



group)

302
G1850

Glycine max

GMHSF29

7.00E−68

302
G1850

Phaseolus

CA902448

4.00E−66




coccineus


302
G1850

Beta vulgaris

BQ583051

6.00E−66

302
G1850

Brassica napus

CD814489

4.00E−64

302
G1850

Citrus sinensis

CB292708

9.00E−62

302
G1850
Oryza sativa ( indica
AAAA01022757

1.00E−60



cultivar-group)

302
G1850

Triticum aestivum

CD894087

1.00E−55

302
G1850

Glycine max

gi2129829

2.10E−66

302
G1850

Lycopersicon

gi100267

1.50E−49




peruvianum


302
G1850

Nicotiana tabacum

gi5821136

1.10E−48

302
G1850

Oryza sativa

gi32482876

4.60E−48



( japonica cultivar-



group)

302
G1850

Oryza sativa

gi16580739

1.60E−42

302
G1850

Lycopersicon

gi100225

9.00E−38




esculentum


302
G1850

Helianthus annuus

gi25052685

7.80E−35

302
G1850

Zea mays

gi2130134

1.40E−33

302
G1850

Phaseolus

gi16118447

1.80E−33




acutifolius


302
G1850

Medicago sativa

gi20162459

8.50E−32

304
G1683

Brassica oleracea

BH582941

5.00E−61

304
G1683

Oryza sativa

AF201895

2.00E−34

304
G1683

Solanum tuberosum

BM404872

3.00E−34

304
G1683

Medicago

AW981431

1.00E−33




truncatula


304
G1683

Glycine max

BI786182

1.00E−33

304
G1683

Oryza sativa

AK103508

2.00E−33



( japonica cultivar-



group)

304
G1683

Lactuca sativa

BQ852906

4.00E−33

304
G1683

Lycopersicon

AW442227

2.00E−32




esculentum


304
G1683

Hordeum vulgare

CA029723

4.00E−32



subsp. vulgare

304
G1683
Oryza sativa ( indica
AAAA01004865

1.00E−31



cultivar-group)

304
G1683

Oryza sativa

gi32492205

1.90E−43



( japonica cultivar-



group)

304
G1683

Oryza sativa

gi6573149

2.40E−39

304
G1683

Solanum

gi32470630

3.90E−39




bulbocastanum


304
G1683

Sorghum bicolor

gi18390099

1.50E−37

304
G1683

Lycopersicon

gi19171209

0.15




esculentum


304
G1683

Pisum sativum

gi7008009

0.75

304
G1683

Zea mays

gi1061308

0.85

304
G1683

Glycine max

gi2129829

0.98

304
G1683
Oryza sativa ( indica
gi4680184

0.99



cultivar-group)

304
G1683

Brassica rapa

gi12655953

1

305
G1893

Glycine max

AW278047.1
1068

305
G1893

Glycine max

GLYMA-28NOV01-
1069




CLUSTER111370_1

305
G1893

Glycine max

GLYMA-28NOV01-
1070




CLUSTER118579_1

305
G1893

Glycine max

GLYMA-28NOV01-
1071




CLUSTER118579_2

305
G1893

Glycine max

GLYMA-28NOV01-
1072




CLUSTER149196_1

305
G1893

Oryza sativa

LIB4309-019-R1-N1-A5
1073

305
G1893

Oryza sativa

OSC101916.C1.p4.fg
1074

305
G1893

Oryza sativa

OSC102096.C1.p3.fg
1075

305
G1893

Oryza sativa

OSC22542.C1.p1.fg
1076

305
G1893

Oryza sativa

OSC25365.C1.pl.fg
1077

305
G1893

Oryza sativa

OSC33187.C1.p5.fg
1078

305
G1893

Oryza sativa

OSC5704.C1.p2.fg
1079

305
G1893

Zea mays

ZEAMA-08NOV01-
1080




CLUSTER288538_1

305
G1893

Zea mays

ZEAMA-08NOV01-
1081




CLUSTER344060_1

305
G1893

Glycine max

Gma_S5146217
1664

305
G1893

Zea mays

Zm_S11445592
1806

305
G1893

Lycopersicon

SGN-UNIGENE-
2037




esculentum

SINGLET-43508

306
G1893

Oryza sativa

AK071104

1.00E−97



( japonica cultivar-



group)

306
G1893

Medicago

CA921097

4.00E−90




truncatula


306
G1893

Helianthus annuus

CD849311

8.00E−90

306
G1893

Glycine max

BU547066

9.00E−89

306
G1893

Gossypium

BQ415977

8.00E−87




arboreum


306
G1893

Populus tremula x

BU825617

2.00E−85




Populus tremuloides


306
G1893

Triticum turgidum

BF293596

7.00E−83

306
G1893

Eschscholzia

CD476634

7.00E−80




californica


306
G1893

Brassica oleracea

BH503569

1.00E−79

306
G1893
Oryza sativa ( indica
AAAA01004319

5.00E−71



cultivar-group)

306
G1893

Oryza sativa

gi15290024

2.20E−78

306
G1893

Oryza sativa

gi20161636

2.20E−78



( japonica cultivar-



group)

306
G1893

Glycine max

gi18376601

1.30E−76

306
G1893

Lycopersicon

gi15984226

2.80E−25




esculentum


306
G1893

Solanum tuberosum

gi563623

1.90E−14

306
G1893

Zea mays

gi3170601

3.40E−13

306
G1893

Nicotiana tabacum

gi4519673

0.31

306
G1893

Brassica rapa

gi29569129

0.79



subsp. pekinensis

306
G1893

Chloroplast

gi13366059

0.94



Fagopyrum sp.



C97106

306
G1893

Pisum sativum

gi2129892

0.97

308
G1917

Brassica oleracea

BH700529

1.00E−76

308
G1917

Gossypium

BG440881

4.00E−53




arboreum


308
G1917

Brassica napus

CD838237

6.00E−45

308
G1917

Glycine max

AW569177

2.00E−32

308
G1917

Medicago

BE240450

7.00E−28




truncatula


308
G1917

Oryza sativa

AK073464

7.00E−25



( japonica cultivar-



group)

308
G1917

Vitis vinifera

CD009085

1.00E−20

308
G1917
Oryza sativa ( indica
AAAA01000157

6.00E−19



cultivar-group)

308
G1917

Oryza sativa

10A19I

6.00E−19

308
G1917

Physcomitrella

AW497013

4.00E−12




patens


308
G1917

Oryza sativa

gi20521225

1.90E−26



( japonica cultivar-



group)

308
G1917

Oryza sativa

gi5091599

1.30E−13

308
G1917

Nicotiana tabacum

gi12711287

3.20E−07

308
G1917

Nicotiana

gi1076609

7.00E−05




plumbaginifolia


308
G1917

Pisum sativum

gi7008009

0.0064

308
G1917

Glycine max

gi913654

0.018

308
G1917

Medicago sativa

gi166376

0.022

308
G1917

Cicer arietinum

gi3893085

0.061

308
G1917

Silene latifolia

gi1628463

0.075

308
G1917

Lycopersicon

gi19322

0.083




esculentum


310
G1923

Medicago

BF649854

5.00E−60




truncatula


310
G1923

Lycopersicon

BI422020

3.00E−59




esculentum


310
G1923

Oryza sativa

AX654704

7.00E−57

310
G1923

Oryza sativa

AK073539

7.00E−57



( japonica cultivar-



group)

310
G1923

Phaseolus

CA897028

9.00E−49




coccineus


310
G1923

Sorghum bicolor

CB927306

1.00E−48

310
G1923

Glycine max

BU926268

3.00E−48

310
G1923

Vitis vinifera

CA810372

1.00E−42

310
G1923

Zea mays

CC629895

5.00E−42

310
G1923

Brassica napus

CD841307

2.00E−39

310
G1923

Oryza sativa

gi20303588

3.50E−57



( japonica cultivar-



group)

310
G1923

Oryza sativa

gi15528779

1.80E−39

310
G1923

Brassica napus

gi31322568

2.90E−39

310
G1923

Phaseolus vulgaris

gi15148914

1.80E−37

310
G1923

Petunia x hybrida

gi21105748

3.80E−37

310
G1923

Solanum tuberosum

gi14485513

6.10E−37

310
G1923

Lycopersicon

gi6175246

3.40E−36




esculentum


310
G1923

Glycine max

gi22597158

9.00E−36

310
G1923
Triticum sp.
gi4218537

1.00E−34

310
G1923

Triticum

gi6732160

1.00E−34




monococcum


311
G1928

Glycine max

GLYMA-28NOV01-
1082




CLUSTER1575_1

311
G1928

Glycine max

GLYMA-28NOV01-
1083




CLUSTER1575_2

311
G1928

Glycine max

GLYMA-28NOV01-
1084




CLUSTER37555_1

311
G1928

Oryza sativa

ORYSA-22JAN02-
1085




CLUSTER2748_3

311
G1928

Oryza sativa

OSC18621.C1.p15.fg
1086

311
G1928

Oryza sativa

OSC8169.C1.p1.fg
1087

311
G1928

Zea mays

ZEAMA-08NOV01-
1088




CLUSTER31066_1

311
G1928

Zea mays

ZEAMA-08NOV01-
1089




CLUSTER483_4

311
G1928

Glycine max

Gma_S4880916
1665

311
G1928

Medicago

Mtr_S5366182
1704




truncatula


311
G1928

Hordeum vulgare

Hv_S151736
1739

311
G1928

Lycopersicon

SGN-UNIGENE-47862
2038




esculentum


311
G1928

Lycopersicon

SGN-UNIGENE-48122
2039




esculentum


311
G1928

Lycopersicon

SGN-UNIGENE-54189
2040




esculentum


311
G1928

Lycopersicon

SGN-UNIGENE-
2041




esculentum

SINGLET-14800

311
G1928

Lycopersicon

SGN-UNIGENE-
2042




esculentum

SINGLET-332512

312
G1928

Brassica napus

BQ704702

1.00E−125

312
G1928

Oryza sativa

AK105663

1.00E−107



( japonica cultivar-



group)

312
G1928

Solanum tuberosum

STPCP1

1.00E−104

312
G1928

Lycopersicon

BI932742

1.00E−96




esculentum


312
G1928

Triticum aestivum

BT009453

1.00E−91

312
G1928

Brassica oleracea

BH958905

6.00E−88

312
G1928

Oryza sativa

AX699673

5.00E−86

312
G1928

Medicago

BG645203

2.00E−85




truncatula


312
G1928

Prunus persica

BU039744

2.00E−84

312
G1928

Glycine max

CA938031

6.00E−83

312
G1928

Oryza sativa

gi10934090

2.40E−108

312
G1928

Solanum tuberosum

gi563623

6.70E−95

312
G1928

Oryza sativa

gi20160482

1.00E−84



( japonica cultivar-



group)

312
G1928

Lycopersicon

gi9858780

1.70E−82




esculentum


312
G1928

Zea mays

gi3170601

4.20E−78

312
G1928

Glycine max

gi18376601

6.60E−18

312
G1928

Capsella rubella

gi32454266

3.60E−06

312
G1928

Chlamydomonas

gi16209575

5.50E−06




reinhardtii


312
G1928

Petunia x hybrida

gi2346972

0.0062

312
G1928

Nicotiana tabacum

gi100367

0.076

314
G1932

Brassica oleracea

BZ451301

2.00E−45

314
G1932

Populus tremula x

BU831322

3.00E−43




Populus tremuloides


314
G1932

Prunus persica

BU044042

3.00E−39

314
G1932

Lotus corniculatus

AP006411

2.00E−37



var. japonicus

314
G1932

Medicago

CF069961

1.00E−35




truncatula


314
G1932

Populus

CA825154

3.00E−32



balsamifera subsp.




trichocarpa x





Populus deltoides


314
G1932

Helianthus annuus

AJ412445

5.00E−31

314
G1932

Lotus japonicus

AV422273

3.00E−30

314
G1932

Glycine max

BM732568

4.00E−30

314
G1932

Lactuca sativa

BQ873409

8.00E−29

314
G1932

Stylosanthes hamata

gi4099921

5.70E−33

314
G1932

Nicotiana tabacum

gi1208496

2.80E−29

314
G1932

Lycopersicon

gi30526297

5.70E−29




esculentum


314
G1932

Nicotiana sylvestris

gi8809573

6.40E−28

314
G1932

Oryza sativa

gi10567106

2.10E−27

314
G1932

Oryza sativa

gi20160965

2.10E−27



( japonica cultivar-



group)

314
G1932

Thellungiella

gi20340233

5.60E−27




halophila


314
G1932

Solanum tuberosum

gi28268684

9.30E−22

314
G1932

Zea mays

gi27802487

1.30E−20

314
G1932

Prunus armeniaca

gi3264767

3.40E−20

316
G1938

Brassica oleracea

BH464032

1.00E−76

316
G1938

Vitis vinifera

CB972449

6.00E−67

316
G1938

Oryza sativa

AP004672

3.00E−52



( japonica cultivar-



group)

316
G1938

Medicago

BF642346

2.00E−47




truncatula


316
G1938

Lactuca sativa

BQ874162

8.00E−45

316
G1938

Solanum tuberosum

BQ507674

3.00E−43

316
G1938

Glycine max

CA937850

1.00E−42

316
G1938

Zea mays

AX540653

5.00E−42

316
G1938

Beta vulgaris

BQ588349

4.00E−33

316
G1938

Gossypium

BG445379

7.00E−33




arboreum


316
G1938

Gossypium

gi5731257

3.20E−32




hirsutum


316
G1938

Oryza sativa

gi2580440

2.00E−29

316
G1938

Oryza sativa

gi20975251

2.60E−29



( japonica cultivar-



group)

316
G1938

Sophora flavescens

gi21624283

2.20E−05

316
G1938

Pueraria montana

gi21624275

0.0003



var. lobata

316
G1938

Bothriochloa

gi13649873

0.00045




odorata


316
G1938

Capillipedium

gi13649864

0.00058




parvalorum


316
G1938

Linaria vulgaris

gi29788713

0.00059

316
G1938

Antirrhinum

gi31296478

0.0013




cornutum


316
G1938

Populus

gi12061239

0.0016



balsamifera subsp.




trichocarpa


318
G1945

Brassica rapa

BG543096

2.00E−85



subsp. pekinensis

318
G1945

Pisum sativum

CD860359

9.00E−69

318
G1945

Brassica oleracea

BH480897

1.00E−66

318
G1945

Glycine max

GD397129

4.00E−66

318
G1945

Medicago

BG647027

4.00E−66




truncatula


318
G1945
Oryza sativa ( indica
AAAA01000383

7.00E−56



cultivar-group)

318
G1945

Oryza sativa

AP005755

9.00E−56



( japonica cultivar-



group)

318
G1945

Helianthus annuus

BU023570

3.00E−52

318
G1945

Zea mays

BZ412041

7.00E−51

318
G1945

Oryza sativa

AP004020

2.00E−48

318
G1945

Oryza sativa

gi32489626

1.60E−47



( japonica cultivar-



group)

318
G1945

Antirrhinum majus

gi4165183

1.20E−21

318
G1945

Pisum sativum

gi2213534

2.20E−14

318
G1945

Helianthus hirsutus

gi27526446

0.091

318
G1945

Helianthus

gi27526452

0.12




tuberosus


318
G1945

Helianthus niveus

gi27526450

0.12

318
G1945

Helianthus ciliaris

gi14588999

0.2

318
G1945

Helianthus praecox

gi18073228

0.25

318
G1945

Helianthus debilis

gi27526440

0.46

318
G1945

Lycopersicon

gi1345538

0.46




esculentum


320
G1957

Brassica oleracea

BZ468915

1.00E−101

320
G1957
Oryza sativa ( indica
AAAA01011764

1.00E−55



cultivar-group)

320
G1957

Oryza sativa

AX654655

2.00E−55

320
G1957

Oryza sativa

AK106367

4.00E−55



( japonica cultivar-



group)

320
G1957

Lactuca sativa

BU005803

1.00E−52

320
G1957

Lycopersicon

BI934637

4.00E−50




esculentum


320
G1957

Glycine max

AW760132

1.00E−49

320
G1957

Zea mays

CC648948

4.00E−48

320
G1957

Solanum tuberosum

BQ119486

2.00E−45

320
G1957

Prunus dulcis

BU645464

3.00E−39

320
G1957

Oryza sativa

gi21426118

1.40E−55



( japonica cultivar-



group)

320
G1957

Marchantia

gi25272004

1.30E−35




polymorpha


320
G1957

Oryza sativa

gi19352051

2.40E−10

320
G1957

Pisum sativum

gi22335711

3.90E−08

320
G1957

Eragrostis tef

gi17906977

1.10E−07

320
G1957

Mangifera indica

gi31747324

1.60E−07

320
G1957

Hordeum vulgare

gi1730475

3.10E−07



subsp. vulgare

320
G1957

Phaseolus vulgaris

gi1046278

3.10E−07

320
G1957

Prunus persica

gi27450533

3.30E−07

320
G1957

Daucus carota

gi5578746

1.20E−06

321
G1968

Glycine max

GLYMA-28NOV01-
1090




CLUSTER38154_1

321
G1968

Glycine max

uC-gmflminsoy034g08b1
1091

321
G1968

Oryza sativa

OSC102229.C1.p13.fg
1092

321
G1968

Oryza sativa

OSC27802.C1.p1.fg
1093

321
G1968

Lycopersicon

SGN-UNIGENE-
2043




esculentum

SINGLET-336915

322
G1968

Brassica oleracea

BH950957

9.00E−85

322
G1968

Petunia x hybrida

AB003672

2.00E−69

322
G1968
Limnanthes alba
BV007314

7.00E−32

322
G1968

Medicago

CB892199

2.00E−18




truncatula


322
G1968
Oryza sativa ( indica
AAAA01000555

7.00E−16



cultivar-group)

322
G1968

Oryza sativa

AP003840

1.00E−15

322
G1968

Triticum aestivum

CA596598

1.00E−14

322
G1968

Solanum tuberosum

BG096505

3.00E−14

322
G1968

Sorghum bicolor

BZ628830

2.00E−12

322
G1968

Zea mays

CC642714

2.00E−12

322
G1968

Petunia x hybrida

gi1786146

9.60E−58

322
G1968

Oryza sativa

gi22775640

2.30E−19



( japonica cultivar-



group)

322
G1968

Pisum sativum

gi2129892

3.00E−09

322
G1968

Glycine max

gi1763063

5.80E−09

322
G1968

Medicago sativa

gi7228329

1.50E−08

322
G1968

Brassica rapa

gi2058504

1.60E−07

322
G1968

Oryza sativa

gi15623826

1.40E−06

322
G1968

Datisca glomerata

gi4666360

2.40E−05

322
G1968

Triticum aestivum

gi485814

9.80E−05

322
G1968

Nicotiana tabacum

gi2981169

0.012

323
G1983

Glycine max

GLYMA-28NOV01-
1094




CLUSTER30038_1

323
G1983

Glycine max

GLYMA-28NOV01-
1095




CLUSTER30038_2

323
G1983

Oryza sativa

ORYSA-22JAN02-
1096




CLUSTER10519_1

323
G1983

Oryza sativa

Os_S120617
1595

323
G1983

Oryza sativa

Os_S16654
1596

323
G1983

Oryza sativa

Os_S44308
1597

323
G1983

Oryza sativa

Os_S71743
1598

323
G1983

Oryza sativa

Os_S75089
1599

323
G1983

Oryza sativa

Os_S77891
1600

323
G1983

Zea mays

Zm_S11326674
1807

323
G1983

Zea mays

Zm_S11446637
1808

323
G1983

Zea mays

Zm_S11525047
1809

323
G1983

Triticum aestivum

Ta_S119681
1885

323
G1983

Triticum aestivum

Ta_S137934
1886

323
G1983

Triticum aestivum

Ta_S142014
1887

323
G1983

Triticum aestivum

Ta_S142165
1888

323
G1983

Triticum aestivum

Ta_S143760
1889

323
G1983

Triticum aestivum

Ta_S147217
1890

323
G1983

Triticum aestivum

Ta_S147350
1891

323
G1983

Triticum aestivum

Ta_S383385
1892

323
G1983

Triticum aestivum

Ta_S412629
1893

323
G1983

Triticum aestivum

Ta_S66264
1894

323
G1983

Triticum aestivum

Ta_S75983
1895

323
G1983

Triticum aestivum

Ta_S78150
1896

323
G1983

Lycopersicon

SGN-UNIGENE-
2044




esculentum

SINGLET-21519

324
G1983

Brassica oleracea

BZ436393

1.00E−120

324
G1983

Brassica napus

CD837762

1.00E−113

324
G1983

Lactuca sativa

BQ850782

8.00E−64

324
G1983

Solanum tuberosum

BG599598

5.00E−61

324
G1983

Populus tremula x

BU866147

2.00E−60




Populus tremuloides


324
G1983
Oryza sativa ( indica
AAAA01006041

5.00E−58



cultivar-group)

324
G1983

Oryza sativa

AP002746

2.00E−56

324
G1983

Oryza sativa

AK106392

2.00E−56



( japonica cultivar-



group)

324
G1983

Glycine max

AW568218

2.00E−56

324
G1983

Medicago

AC144766

3.00E−54




truncatula


324
G1983

Oryza sativa

gi9988428

2.20E−55

324
G1983

Oryza sativa

gi28273376

1.40E−51



( japonica cultivar-



group)

324
G1983

Brassica oleracea

gi15054380

0.00024

324
G1983

Glycine max

gi18736

0.021

324
G1983

Pinus pinaster

gi18129298

0.49

324
G1983

Atropa belladonna

gi14329820

0.58

324
G1983

Pisum sativum

gi31580860

0.58

324
G1983

Pyrus communis

gi559557

0.78

324
G1983

Zea mays

gi4321762

0.81

324
G1983

Gossypioides kirkii

gi29836513

0.84

325
G1985

Glycine max

GLYMA-28NOV01-
1097




CLUSTER65388_1

326
G1985

Medicago

BE124794

3.00E−13




truncatula


326
G1985

Lotus japonicus

AP006103

5.00E−12

326
G1985

Lotus corniculatus

CB826842

3.00E−11



var. japonicus

326
G1985

Brassica oleracea

BH732988

3.00E−11

326
G1985

Brassica rapa

BZ614339

5.00E−10



subsp. pekinensis

326
G1985

Petunia x hybrida

AB035093

1.00E−09

326
G1985

Glycine max

AI973860

1.00E−09

326
G1985
Oryza sativa ( indica
AAAA01001411

3.00E−09



cultivar-group)

326
G1985

Oryza sativa

AP005869

3.00E−09



( japonica cultivar-



group)

326
G1985

Zea mays

CC652748

4.00E−09

326
G1985

Petunia x hybrida

gi14275902

1.60E−15

326
G1985

Oryza sativa

gi32489630

6.50E−13



( japonica cultivar-



group)

326
G1985

Zea ramosa

gi18674684

2.90E−11

326
G1985

Oryza sativa

gi15528588

2.70E−05

326
G1985

Sorghum bicolor

gi18390109

0.00088

326
G1985

Brassica rapa

gi2058504

0.001

326
G1985

Pisum sativum

gi2129892

0.0076

326
G1985

Datisca glomerata

gi4666360

0.051

326
G1985

Nicotiana tabacum

gi2981169

0.053

326
G1985

Medicago sativa

gi7228329

0.07

327
G1988

Glycine max

GLYMA-28NOV01-
1098




CLUSTER75453_1

327
G1988

Glycine max

GLYMA-28NOV01-
1099




CLUSTER75453_2

327
G1988

Oryza sativa

ORYSA-22JAN02-
1100




CLUSTER153439_2

327
G1988

Zea mays

ZEAMA-08NOV01-
1101




CLUSTER10890_1

327
G1988

Zea mays

ZEAMA-08NOV01-
1102




CLUSTER10890_3

327
G1988

Zea mays

ZEAMA-08NOV01-
1103




CLUSTER201962_1

327
G1988

Zea mays

ZEAMA-08NOV01-
1104




CLUSTER3040_3

327
G1988

Oryza sativa

Os_S91481
1601

327
G1988

Lycopersicon

SGN-UNIGENE-
2045




esculentum

SINGLET-5090

328
G1988

Brassica oleracea

BH478747

5.00E−23

328
G1988

Populus

BU873581

7.00E−22



balsamifera subsp.




trichocarpa


328
G1988

Citrus unshiu

C95300

2.00E−18

328
G1988

Lycopersicon

AW034552

2.00E−18




esculentum


328
G1988
Oryza sativa ( indica
AAAA01000340

1.00E−17



cultivar-group)

328
G1988

Beta vulgaris

BQ594583

1.00E−16

328
G1988

Zea mays

CC655765

2.00E−15

328
G1988

Glycine max

BI469275

8.00E−15

328
G1988

Prunus persica

BU046688

7.00E−14

328
G1988

Vitis vinifera

CD719941

2.00E−13

328
G1988

Malus x domestica

gi4091806

2.60E−07

328
G1988

Brassica napus

gi30984027

1.10E−06

328
G1988

Brassica nigra

gi22854920

1.10E−06

328
G1988

Raphanus sativus

gi3341723

2.70E−06

328
G1988

Oryza sativa

gi32488104

4.80E−06



( japonica cultivar-



group)

328
G1988

Ipomoea nil

gi10946337

5.10E−06

328
G1988

Oryza sativa

gi11094211

2.20E−05

328
G1988

Hordeum vulgare

gi21667475

4.50E−05

328
G1988

Hordeum vulgare

gi21655168

0.00018



subsp. vulgare

328
G1988

Pinus radiata

gi4557093

0.0016

330
G1990

Brassica oleracea

BH009262

2.00E−99

330
G1990

Petunia x hybrida

AB000452

9.00E−44

330
G1990

Populus

BU879483

1.00E−42



balsamifera subsp.




trichocarpa


330
G1990

Lotus japonicus

AP004479

4.00E−39

330
G1990

Medicago

AC126007

7.00E−35




truncatula


330
G1990

Oryza sativa

AP003989

2.00E−32

330
G1990
Oryza sativa ( indica
AAAA01004471

2.00E−32



cultivar-group)

330
G1990

Ipomoea nil

BJ573446

6.00E−32

330
G1990

Pisum sativum

PSZINCFIN

1.00E−31

330
G1990

Populus tremula x

BU887507

3.00E−31




Populus tremuloides


330
G1990

Pisum sativum

gi2129892

3.90E−42

330
G1990

Petunia x hybrida

gi100396

1.30E−40

330
G1990

Oryza sativa

gi32480087

1.40E−23



( japonica cultivar-



group)

330
G1990

Oryza sativa

gi15623820

1.10E−18

330
G1990

Triticum aestivum

gi485814

2.30E−18

330
G1990

Brassica rapa

gi2058506

2.50E−15

330
G1990

Glycine max

gi1763063

3.30E−14

330
G1990

Datisca glomerata

gi4666360

1.80E−08

330
G1990

Medicago sativa

gi7228329

2.20E−06

330
G1990

Nicotiana tabacum

gi2981169

1.40E−05

332
G1993

Lotus japonicus

AP006103

7.00E−12

332
G1993

Medicago

CF069502

1.00E−11




truncatula


332
G1993

Lotus corniculatus

CB826842

3.00E−11



var. japonicus

332
G1993

Petunia x hybrida

A8035093

6.00E−10

332
G1993

Brassica oleracea

BH732988

6.00E−10

332
G1993

Glycine max

AI973860

1.00E−09

332
G1993

Brassica rapa

BZ614339

7.00E−09



subsp. pekinensis

332
G1993
Oryza sativa ( indica
AAAA01001411

3.00E−08



cultivar-group)

332
G1993

Oryza sativa

AP005869

3.00E−08



( japonica cultivar-



group)

332
G1993

Zea mays

BZ735433

5.00E−08

332
G1993

Petunia x hybrida

gi14275902

4.30E−15

332
G1993

Oryza sativa

gi27261062

8.20E−13



( japonica cultivar-



group)

332
G1993

Zea ramosa

gi18674684

6.00E−11

332
G1993

Oryza sativa

gi15528588

3.50E−08

332
G1993

Sorghum bicolor

gi18390109

8.80E−06

332
G1993

Brassica rapa

gi2058504

0.0056

332
G1993

Medicago sativa

gi7228329

0.084

332
G1993

Datisca glomerata

gi4666360

0.12

332
G1993

Nicotiana tabacum

gi2981169

0.14

332
G1993

Pisum sativum

gi2129892

0.38

333
G1995

Glycine max

GLYMA-28NOV01-
753




CLUSTER166362_1

333
G1995

Glycine max

GLYMA-28NOV01-
754




CLUSTER180202_1

333
G1995

Glycine max

GLYMA-28NOV01-
755




CLUSTER726571_1

333
G1995

Glycine max

GLYMA-28NOV01-
756




CLUSTER74662_1

333
G1995

Glycine max

uC-gmflminsoy032f06b1
757

333
G1995

Oryza sativa

ORYSA-22JAN02-
759




CLUSTER200967_1

333
G1995

Oryza sativa

OSC100895.C1.p14.fg
760

333
G1995

Oryza sativa

OSC23411.C1.p1.fg
761

333
G1995

Oryza sativa

OSC2409.C1.p2.fg
762

333
G1995

Oryza sativa

OSC25680.C1.p1.fg
763

333
G1995

Zea mays

ZEAMA-08NOV01-
764




CLUSTER436044_1

333
G1995

Zea mays

ZEAMA-08NOV01-
765




CLUSTER518126_1

333
G1995

Lycopersicon

SGN-UNIGENE-54039
1959




esculentum


333
G1995

Lycopersicon

SGN-UNIGENE-54252
1960




esculentum


333
G1995

Lycopersicon

SGN-UNIGENE-
1961




esculentum

SINGLET-392715

334
G1995

Brassica oleracea

BZ475765

1.00E−68

334
G1995

Lycopersicon

BG123251

3.00E−30




esculentum


334
G1995

Vitis vinifera

CD716644

1.00E−29

334
G1995

Gossypium

BF272143

2.00E−27




arboreum


334
G1995

Oryza sativa

AP005538

4.00E−24



( japonica cultivar-



group)

334
G1995
Oryza sativa ( indica
AAAA01009505

9.00E−23



cultivar-group)

334
G1995

Oryza sativa

AC105732

1.00E−18

334
G1995

Zea mays

BH875187

2.00E−18

334
G1995

Hordeum vulgare

BF616974

3.00E−18

334
G1995

Sorghum bicolor

BE360413

4.00E−17

334
G1995

Sorghum bicolor

gi18390109

4.30E−20

334
G1995

Oryza sativa

gi15528588

5.80E−08

334
G1995

Oryza sativa

gi32482926

1.90E−07



( japonica cultivar-



group)

334
G1995

Petunia x hybrida

gi2346976

1.30E−05

334
G1995

Glycine max

gi1763063

0.0043

334
G1995

Medicago sativa

gi7228329

0.007

334
G1995

Zea ramosa

gi18674684

0.0093

334
G1995

Datisca glomerata

gi4666360

0.082

334
G1995

Brassica rapa

gi2058506

0.098

334
G1995

Triticum aestivum

gi485814

0.24

336
G1998

Oryza sativa

AK071630

3.00E−53



( japonica cultivar-



group)

336
G1998

Oryza sativa

AB001888

3.00E−53

336
G1998

Triticum aestivum

BJ209915

1.00E−30

336
G1998

Hordeum vulgare

BE558327

4.00E−30

336
G1998
Oryza sativa ( indica
AAAA01003074

2.00E−29



cultivar-group)

336
G1998

Zea mays

BZ985999

4.00E−29

336
G1998

Oryza minuta

CB210857

1.00E−28

336
G1998

Lactuca sativa

BU009733

3.00E−28

336
G1998

Medicago

BG644908

1.00E−27




truncatula


336
G1998

Solanum tuberosum

BQ121038

5.00E−27

336
G1998

Oryza sativa

gi3618320

9.60E−58

336
G1998

Brassica nigra

gi22854986

1.70E−25

336
G1998
Raphanus sativus
gi3341723

1.00E−23

336
G1998

Ipomoea nil

gi10946337

1.00E−23

336
G1998

Malus x domestica

gi4091806

1.20E−22

336
G1998

Brassica napus

gi2895184

2.80E−22

336
G1998

Oryza sativa

gi23589949

1.20E−21



( japonica cultivar-



group)

336
G1998

Hordeum vulgare

gi21667475

1.80E−21

336
G1998

Pinus radiata

gi4557093

2.50E−20

336
G1998

Hordeum vulgare

gi21655154

1.60E−18



subsp. vulgare

338
G1999

Medicago

BE316747

3.00E−22




truncatula


338
G1999

Lactuca sativa

BQ995915

1.00E−20

338
G1999

Lycopersicon

AI772841

5.00E−20




esculentum


338
G1999

Brassica oleracea

BH927868

9.00E−20

338
G1999

Mesembryanthemum

CA840421

2.00E−19




crystallinum


338
G1999

Brassica napus

A50832

2.00E−18

338
G1999

Beta vulgaris

BQ489587

1.00E−17

338
G1999

Eschscholzia

CD477071

2.00E−16




californica


338
G1999

Glycine max

CD401749

1.00E−15

338
G1999

Ipomoea nil

BJ554943

3.00E−15

338
G1999

Oryza sativa

gi13702811

1.10E−27

338
G1999

Malus x domestica

gi4091806

2.10E−20

338
G1999

Raphanus sativus

gi3341723

4.80E−19

338
G1999

Brassica nigra

gi22854916

8.80E−18

338
G1999

Oryza sativa

gi23589949

1.30E−17



( japonica cultivar-



group)

338
G1999

Brassica napus

gi2895184

1.30E−17

338
G1999

Hordeum vulgare

gi21667471

2.70E−17

338
G1999

Hordeum vulgare

gi21655168

5.00E−17



subsp. vulgare

338
G1999

Pinus radiata

gi4557093

6.00E−17

338
G1999

Ipomoea nil

gi10946337

2.80E−16

340
G2035

Medicago

AC140035

1.00E−115




truncatula


340
G2035

Brassica oleracea

BZ428219

1.00E−106

340
G2035

Oryza sativa

AK100495

1.00E−102



( japonica cultivar-



group)

340
G2035

Zea mays

AX660947

1.00E−100

340
G2035

Vitis vinifera

CB979115

5.00E−96

340
G2035

Oryza sativa

AG084405

9.00E−95

340
G2035

Glycine max

AW186423

7.00E−92

340
G2035
Oryza sativa ( indica
AAAA01010916

1.00E−89



cultivar-group)

340
G2035

Lycopersicon

BF113841

4.00E−82




esculentum


340
G2035

Lactuca sativa

BQ865775

3.00E−81

340
G2035

Oryza sativa

gi24796797

4.60E−103



( japonica cultivar-



group)

340
G2035

Oryza sativa

gi14140292

3.30E−09

340
G2035

Populus tremula x

gi9955730

4.10E−05




Populus tremuloides


340
G2035

Nicotiana

gi5834502

5.20E−05




paniculata


340
G2035

Solanum tuberosum

gi2225999

0.00011

340
G2035

Samanea saman

gi5081693

0.00029

340
G2035

Chlamydomonas

gi30025990

0.0011




reinhardtii


340
G2035

Vicia faba

gi2293112

0.0045

340
G2035

Triticum aestivum

gi18616499

0.013

340
G2035

Brassica napus

gi22003730

0.022

341
G2041

Glycine max

GLYMA-28NOV01-
1105




CLUSTER244491_1

341
G2041

Glycine max

LIB4280-051-Q1-K1-E4
1106

341
G2041

Oryza sativa

rsicem_7360.y1.abd
1107

341
G2041

Zea mays

Zm_S11428605
1810

341
G2041

Lycopersicon

SGN-UNIGENE-47127
2046




esculentum


341
G2041

Lycopersicon

SGN-UNIGENE-
2047




esculentum

SINGLET-389924

342
G2041

Glycine max

AX196296

1.0e−999

342
G2041
Oryza sativa ( indica
AAAA01023044

1.00E−161



cultivar-group)

342
G2041

Oryza sativa

AP004333

1.00E−161



( japonica cultivar-



group)

342
G2041

Oryza sativa

AC107085

8.00E−90

342
G2041

Lotus corniculatus

AP006426

7.00E−89



var. japonicus

342
G2041

Medicago

BZ286591

9.00E−89




truncatula


342
G2041

Helianthus annuus

CD853758

2.00E−88

342
G2041

Lactuca sativa

BQ853515

6.00E−87

342
G2041

Capsicum annuum

BM067036

3.00E−82

342
G2041

Lycopersicon

BI925244

8.00E−79




esculentum


342
G2041

Oryza sativa

gi33146888

1.50E−152



( japonica cultivar-



group)

342
G2041

Oryza sativa

gi14140291

5.60E−34

342
G2041

Zea mays

gi18463957

1.50E−19

342
G2041

Hordeum vulgare

gi23193481

4.40E−08

342
G2041

Hordeum vulgare

gi23193479

1.40E−07



subsp. vulgare

342
G2041

Triticum

gi23193487

2.60E−07




monococcum


342
G2041

Brassica napus

gi4106378

0.12

342
G2041

Medicago sativa

gi1279563

1

342
G2041

Nicotiana tabacum

gi8096269

1

342
G2041

Triticum aestivum

gi32400814

1

343
G2051

Medicago

Mtr_S5387033
1705




truncatula


343
G2051

Hordeum vulgare

Hv_S171660
1740

344
G2051

Brassica oleracea

BH456374

6.00E−78

344
G2051

Petunia x hybrida

AF509874

4.00E−69

344
G2051

Lycopersicon

AW222093

2.00E−57




esculentum


344
G2051

Oryza sativa

AX654515

1.00E−51

344
G2051

Medicago

BQ165266

7.00E−51




truncatula


344
G2051

Sorghum bicolor

BG933492

8.00E−50

344
G2051

Oryza sativa

AK099540

5.00E−43



( japonica cultivar-



group)

344
G2051

Hordeum vulgare

BJ481205

5.00E−42



subsp. spontaneum

344
G2051

Hordeum vulgare

BQ469035

5.00E−42

344
G2051

Hordeum vulgare

BU967516

5.00E−42



subsp. vulgare

344
G2051

Petunia x hybrida

gi21105751

9.50E−70

344
G2051

Oryza sativa

gi19225018

3.40E−59



( japonica cultivar-



group)

344
G2051

Oryza sativa

gi6730946

1.20E−45

344
G2051

Brassica napus

gi31322582

3.50E−41

344
G2051

Lycopersicon

gi6175246

8.40E−40




esculentum


344
G2051

Phaseolus vulgaris

gi15148914

1.10E−39

344
G2051

Solanum tuberosum

gi14485513

3.30E−38

344
G2051

Medicago

gi7716952

3.30E−38




truncatula


344
G2051

Glycine max

gi22597158

8.70E−38

344
G2051
Triticum sp.
gi4218537

8.70E−38

345
G2060

Oryza sativa

Os_S109370
1602

345
G2060

Medicago

Mtr_S5408429
1706




truncatula


345
G2060

Triticum aestivum

Ta_S317703
1897

346
G2060

Oryza sativa

AX653450

4.00E−49

346
G2060

Brassica oleracea

BH456149

7.00E−48

346
G2060

Lycopersicon

BI422854

1.00E−39




esculentum


346
G2060

Nicotiana tabacum

AY220477

1.00E−36

346
G2060

Citrus sinensis

BQ625082

2.00E−35

346
G2060

Lactuca sativa

BQ870137

3.00E−35

346
G2060

Hordeum vulgare

BM370908

9.00E−35

346
G2060

Medicago

CB893379

2.00E−34




truncatula


346
G2060

Glycine max

CA782643

8.00E−34

346
G2060

Cryptomeria

AU083645

2.00E−33




japonica


346
G2060

Oryza sativa

gi11320830

2.60E−40

346
G2060

Nicotiana tabacum

gi30013667

5.30E−38

346
G2060

Oryza sativa

gi20160973

2.10E−27



( japonica cultivar-



group)

346
G2060

Petroselinum

gi11493822

4.50E−21




crispum


346
G2060

Avena fatua

gi1159879

3.00E−19

346
G2060
Oryza sativa ( indica
gi23305051

2.60E−17



cultivar-group)

346
G2060

Pimpinella

gi3420906

2.60E−16




brachycarpa


346
G2060

Avena sativa

gi4894965

7.10E−16

346
G2060

Ipomoea batatas

gi1076685

1.20E−15

346
G2060

Solanum tuberosum

gi24745606

2.20E−15

348
G2063

Brassica oleracea

BH923036

3.00E−45

348
G2063

Medicago

AC139746

1.00E−18




truncatula


348
G2063

Lotus corniculatus

AP006395

2.00E−18



var. japonicus

348
G2063

Gossypium

BQ403135

2.00E−17




arboreum


348
G2063

Oryza sativa

AP004093

4.00E−17

348
G2063

Oryza sativa

AP004863

4.00E−17



( japonica cultivar-



group)

348
G2063

Lotus japonicus

AP006142

5.00E−16

348
G2063

Sorghum bicolor

BZ627051

8.00E−16

348
G2063

Zea mays

CG690653

5.00E−15

348
G2063

Brassica napus

CD813986

1.00E−14

348
G2063

Oryza sativa

gi15290141

1.90E−17

343
G2063

Antirrhinum majus

gi264223

5.80E−17

348
G2063

Picea abies

gi25307922

2.00E−15

348
G2063

Gossypium

gi19743774

3.20E−15




hirsutum


348
G2063

Malus x domestica

gi16973298

5.30E−15

348
G2063

Phalaenopsis

gi18650789

1.10E−14




equestris


348
G2063

Oryza sativa

gi33090203

1.40E−14



( japonica cultivar-



group)

348
G2063

Petunia x hybrida

gi17827467

1.80E−14

348
G2063

Nicotiana tabacum

gi3913007

1.80E−14

348
G2063

Cucumis sativus

gi13810204

2.30E−14

350
G2070

Brassica oleracea

BH598265

1.00E−44

350
G2070

Populus

BU870843

2.00E−14



balsamifera subsp.




trichocarpa


350
G2070

Lycopersicon

BI922075

1.00E−12




esculentum


350
G2070

Brassica napus

GD833815

1.00E−12

350
G2070

Glycine max

BM269595

6.00E−11

350
G2070

Ipomoea nil

BJ572296

2.00E−10

350
G2070
Oryza sativa ( indica
AAAA01003396

6.00E−10



cultivar-group)

350
G2070

Prunus armeniaca

CB821535

1.00E−09

350
G2070

Nicotiana tabacum

AY045572

1.00E−09

350
G2070

Oryza sativa

AP005785

1.00E−09



( japonica cultivar-



group)

350
G2070

Nicotiana tabacum

gi1658G134

3.00E−12

350
G2070

Phaseolus vulgaris

gi13430400

1.00E−11

350
G2070

Phaseolus

gi12829956

2.70E−11




acutifolius


350
G2070

Petroselinum

gi9650826

1.00E−09




crispum


350
G2070

Hordeum vulgare

gi1869928

1.40E−09

350
G2070

Antirrhinum majus

gi2244742

3.60E−09

350
G2070

Capsicum chinense

gi4457221

9.40E−09

350
G2070

Glycine max

gi1905785

1.00E−08

350
G2070

Oryza sativa

gi18698991

1.10E−08

350
G2070

Zea mays

gi1060935

1.20E−08

352
G2071

Vitis vinifera

VVI237992

4.00E−87

352
G2071

Phaseolus vulgaris

AF369792

3.00E−77

352
G2071

Brassica oleracea

BZ007832

4.00E−75

352
G2071

Nicotiana tabacum

AB063648

2.00E−74

352
G2071

Oryza sativa

AK072062

9.00E−65



( japonica cultivar-



group)

352
G2071

Hordeum vulgare

AY150676

3.00E−58



subsp. vulgare

352
G2071

Oryza sativa

AB023288

1.00E−52

352
G2071
Oryza sativa ( indica
AAAA01010740

4.00E−47



cultivar-group)

352
G2071

Helianthus annuus

AF001453

8.00E−40

352
G2071

Triticum aestivum

AFS19804

6.00E−38

352
G2071

Vitis vinifera

gi7406677

2.30E−87

352
G2071

Hordeum vulgare

gi27469352

1.40E−64



subsp. vulgare

352
G2071

Nicotiana tabacum

gi14571808

1.50E−58

352
G2071

Oryza sativa

gi33087069

3.20E−53



( japonica cultivar-



group)

352
G2071

Oryza sativa

gi5821255

3.20E−53

352
G2071

Phaseolus vulgaris

gi13775111

1.80E−46

352
G2071

Triticum aestivum

gi21693585

2.50E−31

352
G2071

Helianthus annuus

gi2228771

1.10E−30

352
G2071

Zea mays

gi15422222

7.60E−08

352
G2071

Populus x generosa

gi13435335

2.50E−07

354
G2084

Brassica oleracea

BH426816

2.00E−77

354
G2084

Triticum aestivum

CD937226

3.00E−41

354
G2084

Poncirus trifoliata

CD574564

8.00E−41

354
G2084

Vitis vinifera

CB981408

1.00E−40

354
G2084

Oryza sativa

AP003314

5.00E−40

354
G2084
Oryza sativa ( indica
AAAA01013746

5.00E−40



cultivar-group)

354
G2084

Zea mays

CD434392

9.00E−40

354
G2084

Oryza sativa

AK066561

1.00E−39



( japonica cultivar-



group)

354
G2084

Sorghum bicolor

BE356071

1.00E−39

354
G2084

Brassica napus

CD837858

3.00E−37

354
G2084

Oryza sativa

gi11034559

3.20E−40

354
G2084

Oryza sativa

gi21104687

3.20E−40



( japonica cultivar-



group)

354
G2084

Pinus pinaster

gi18129286

0.65

354
G2084

Pinus thunbergii

gi1262717

0.94

354
G2084

Chloroplast Pinus

gi7484547

0.94




thunbergii


354
G2084

Glycine max

gi21439778

0.98

354
G2084

Nicotiana tabacum

gi478508

1

354
G2084

Chloroplast

gi544746

1




Nicotiana sylvestris


354
G2084

Chloroplast

gi544747

1




Nicotiana tabacum


354
G2084

Ipomoea batatas

gi100467

1

355
G2085

Glycine max

GLYMA-28NOV01-
1108




CLUSTER62196_1

355
G2085

Glycine max

GLYMA-28NOV01-
1109




CLUSTER65589_1

355
G2085

Glycine max

GLYMA-28NOV01-
1110




CLUSTER65689_2

355
G2085

Glycine max

jC-
1111




gmXLIB3563P057de07d2

355
G2085

Oryza sativa

OSC102337.C1.p1.fg
1112

355
G2085

Zea mays

ZEAMA-08NOV01-
1113




CLUSTER841917_1

355
G2085

Medicago

Mtr_S10820675
1707




truncatula


356
G2085

Glycine max

BI498544

1.00E−58

356
G2085

Medicago

CA991109

1.00E−54




truncatula


356
G2085

Vitis vinifera

BM437375

1.00E−46

356
G2085

Triticum aestivum

BQ295376

1.00E−44

356
G2085

Triticum

BF199732

9.00E−44




monococcum


356
G2085

Oryza sativa

AK068931

2.00E−43



( japonica cultivar-



group)

356
G2085

Zea mays

AY103800

3.00E−43

356
G2085

Brassica oleracea

BH723453

3.00E−40

356
G2085

Hordeum vulgare

BU993000

5.00E−39

356
G2085

Ipomoea nil

BJ572579

1.00E−38

356
G2085

Oryza sativa

gi13174240

3.90E−42

356
G2085

Oryza sativa

gi24960749

1.80E−07



( japonica cultivar-



group)

356
G2085

Brassica rapa

gi28193631

2.90E−06



subsp. pekinensis

356
G2085

Nicotiana tabacum

gi12711287

0.00075

356
G2085

Hordeum vulgare

gi21655162

0.0033



subsp. vulgare

356
G2085

Nicotiana

gi1076609

0.019




plumbaginifolia


356
G2085

Brassica nigra

gi22854920

0.02

356
G2085

Brassica napus

gi30984027

0.091

356
G2085

Raphanus sativus

gi3341723

0.1

356
G2085

Pisum sativum

gi27530710

0.13

358
G2106

Citrus sinensis

BQ625052

8.00E−92

358
G2106

Oryza sativa

AK106769

3.00E−88



( japonica cultivar-



group)

358
G2106

Zea mays

AY103852

3.00E−74

358
G2106

Brassica napus

GD832004

6.00E−74

358
G2106

Lycopersicon

AW030921

3.00E−70




esculentum


358
G2106

Physcomitrella

BJ178045

5.00E−68



patens subsp. patens

358
G2106

Glycine max

CA783156

5.00E−67

358
G2106

Oryza sativa

AX555220

6.00E−66

358
G2106

Nuphar advena

CD475882

3.00E−65

358
G2106

Lactuca sativa

BQ864461

7.00E−58

358
G2106

Brassica napus

gi21069053

5.10E−65

358
G2106

Oryza sativa

gi25898749

7.40E−64

358
G2106

Glycine max

gi25898747

7.40E−64

358
G2106

Oryza sativa

gi20161013

2.00E−63



( japonica cultivar-



group)

358
G2106

Zea mays

gi2652938

1.10E−62

358
G2106

Antirrhinum majus

gi28894443

1.90E−39

358
G2106

Malus x domestica

gi21717332

2.30E−35

358
G2106

Hordeum vulgare

gi18476518

2.40E−35

358
G2106

Picea abies

gi11181612

4.00E−34

358
G2106

Petunia x hybrida

gigi5081555

7.20E−34

359
G2109

Oryza sativa

uC-osflM202145h04b1
1114

359
G2109

Zea mays

LIB3066-034-Q1-K1-
1115




BI2

359
G2109

Oryza sativa

Os_S114273
1603

359
G2109

Oryza sativa

Os_S29604
1604

359
G2109

Zea mays

Zm_S11374620
1811

359
G2109

Triticum aestivum

Ta_S302577
1898

360
G2109

Brassica napus

CD815586

1.00E−50

360
G2109

Beta vulgaris

BQ583447

5.00E−45

360
G2109

Ceratopteris

BE643398

9.00E−33




richardii


360
G2109

Nicotiana tabacum

AY183721

2.00E−31

360
G2109

Physcomitrella

PPA419329

5.00E−28




patens


360
G2109

Physcomitrella

AB067689

1.00E−22



patens subsp. patens

360
G2109

Brassica oleracea

BH451078

6.00E−20

360
G2109

Zinnia elegans

AU287699

9.00E−17

360
G2109

Oryza sativa

AC120888

1.00E−16



( japonica cultivar-



group)

360
G2109

Oryza sativa

AC108870

1.00E−16

360
G2109

Nicotiana tabacum

gi27802107

1.40E−34

360
G2109

Physcomitrella

gi22474457

8.50E−32




patens


360
G2109

Physcomitrella

gi22090622

9.30E−29



patens subsp. patens

360
G2109
Gerbera hybrid cv.
gi29500904

6.10E−21



‘ Terra Regina ’

360
G2109

Triticum aestivum

gi30721847

1.50E−20

360
G2109

Populus

gi10835358

1.70E−20



balsamifera subsp.




trichocarpa


360
G2109

Zea mays

gi29372764

3.60E−20

360
G2109

Triticum

gi30090030

4.00E−20




monococcum


360
G2109

Oryza sativa

gi31712055

4.20E−20



( japonica cultivar-



group)

360
G2109

Papaver nudicaule

gi3170464

9.70E−20

362
G2111

Glycine max

AW508033

3.00E−35

362
G2111

Brassica oleracea

BH455618

2.00E−32

362
G2111

Medicago

AC137602

2.00E−29




truncatula


362
G2111

Antirrhinum majus

AJ558896

9.00E−27

362
G2111

Gossypium

BE054256

4.00E−25




arboreum


362
G2111

Gossypium

BH023181

9.00E−24




hirsutum


362
G2111

Zea mays

CC654475

5.00E−23

362
G2111

Oryza sativa

AP002070

9.00E−22

362
G2111
Oryza sativa ( indica
AAAA01000422

9.00E−22



cultivar-group)

362
G2111

Lotus japonicus

AP004625

2.00E−18

362
G2111

Oryza sativa

gi8096379

1.80E−23

362
G2111
Gerbera hybrid cv.
gi29500904

7.70E−14



‘ Terra Regina ’

362
G2111

Nicotiana tabacum

gi4102113

1.30E−13

362
G2111

Brassica oleracea

gi23304676

1.60E−13



var. botrytis

362
G2111

Lycopersicon

gi20219014

8.90E−13




esculentum


362
G2111

Sinapis alba

gi1076477

1.10E−12

362
G2111

Oryza sativa

gi21742221

1.80E−12



( japonica cultivar-



group)

362
G2111

Lolium perenne

gi28630953

1.80E−12

362
G2111

Triticum

gi30090030

2.40E−12




monococcum


362
G2111

Triticum aestivum

gi30721847

2.40E−12

364
G2129

Brassica napus

CD833815

8.00E−49

364
G2129

Brassica oleracea

BH972911

4.00E−38

364
G2129

Populus

BU870843

4.00E−26



balsamifera subsp.




trichocarpa


364
G2129

Glycine max

BM269595

3.00E−22

364
G2129

Lycopersicon

BF051268

3.00E−21




esculentum


364
G2129

Beta vulgaris

BQ582406

2.00E−11

364
G2129

Gossypium

BQ407558

4.00E−11




arboreum


364
G2129

Medicago

BG648225

4.00E−10




truncatula


364
G2129

Oryza sativa

AK098869

1.00E−09



( japonica cultivar-



group)

364
G2129
Oryza sativa ( indica
CA759739

1.00E−09



cultivar-group)

364
G2129

Nicotiana tabacum

gi16580130

1.30E−11

364
G2129

Petroselinum

gi9650828

2.70E−11




crispum


364
G2129

Capsicum chinense

gi24460973

4.40E−11

364
G2129

Phaseolus vulgaris

gi13430400

7.20E−11

364
G2129

Lycopersicon

gi5901747

7.20E−11




esculentum


364
G2129

Phaseolus

gi12829956

1.20E−10




acutifolius


364
G2129

Antirrhinum majus

gi2244742

1.20E−10

364
G2129

Zea mays

gi1352613

1.90E−10

364
G2129

Glycine max

gi22597162

3.10E−10

364
G2129

Sorghum bicolor

gi1076760

3.60E−10

365
G2142

Glycine max

GLYMA-28NOV01-
1116




CLUSTER10684_8

365
G2142

Glycine max

GLYMA-28NOV01-
1117




CLUSTER137024_1

365
G2142

Glycine max

GLYMA-28NOV01-
1118




CLUSTER49853_1

365
G2142

Glycine max

GLYMA-28NOV01-
1119




CLUSTER49853_4

365
G2142

Glycine max

LIB3242-451-P1-J1-G8
1120

365
G2142

Glycine max

jC-
1121




gmXLIB3S63P042ag07d1

365
G2142

Oryza sativa

ORYSA-22JAN02-
1122




CLUSTER54709_1

365
G2142

Oryza sativa

ORYSA-22JAN02-
1123




CLUSTER8097_1

365
G2142

Zea mays

700164501H1
1124

365
G2142

Glycine max

Gma_S4891278
1666

365
G2142

Medicago

Mtr_S5397469
1708




truncatula


365
G2142

Zea mays

Zm_S11527973
1812

365
G2142

Triticum aestivum

Ta_S115402
1899

365
G2142

Triticum aestivum

Ta_S146851
1900

365
G2142

Triticum aestivum

Ta_S308126
1901

365
G2142

Lycopersicon

SGN-UNIGENE-48174
2048




esculentum


365
G2142

Lycopersicon

SGN-UNIGENE-50424
2049




esculentum


365
G2142

Lycopersicon

SGN-UNIGENE-56397
2050




esculentum


365
G2142

Lycopersicon

SGN-UNIGENE-56608
2051




esculentum


366
G2142

Brassica napus

CD813318

8.00E−90

366
G2142

Medicago

BF650735

2.00E−59




truncatula


366
G2142

Populus tremula x

BU837621

4.00E−59




Populus tremuloides


366
G2142

Glycine max

BU080678

3.00E−58

366
G2142

Beta vulgaris

BQ594352

4.00E−54

366
G2142

Solanum tuberosum

BF186943

6.00E−53

366
G2142

Lycopersicon

AI490572

1.00E−52




esculentum


366
G2142

Oryza sativa

AK101896

7.00E−48



( japonica cultivar-



group)

366
G2142

Stevia rebaudiana

BG524015

2.00E−44

366
G2142

Hordeum vulgare

BU989763

8.00E−42



subsp. vulgare

366
G2142

Pennisetum

gi527655

3.10E−10




glaucum


366
G2142

Sorghum bicolor

gi527665

3.90E−08

366
G2142

Phyllostachys acuta

gi527661

6.50E−08

366
G2142

Tripsacum australe

gi527663

1.80E−07

366
G2142

Oryza sativa

gi32488806

3.20E−07



( japonica cultivar-



group)

366
G2142

Oryza sativa

gi15451582

3.50E−07

366
G2142

Oryza rufipogon

gi2130061

6.40E−07

366
G2142

Oryza australiensis

gi1086526

1.40E−06

366
G2142

Oryza officinalis

gi1086534

2.90E−06

366
G2142

Oryza

gi1086530

3.80E−06




longistaminata


368
G2146

Brassica rapa

BG543943

8.00E−48



subsp. pekinensis

368
G2146

Brassica oleracea

BH978369

2.00E−42

368
G2146

Lotus japonicus

BI418128

6.00E−29

368
G2146

Glycine max

CD398757

4.00E−28

368
G2146

Poncirus trifoliata

CD575942

2.00E−27

368
G2146

Gossypium

BE054359

8.00E−26




arboreum


368
G2146

Oryza sativa

AK103709

1.00E−25



( japonica cultivar-



group)

368
G2146

Oryza sativa

AF461424

1.00E−25

368
G2146

Ipomoea nil

BJ574783

7.00E−25

368
G2146

Populus tremula x

BU832777

3.00E−24




Populus tremuloides


368
G2146

Oryza sativa

gi24059889

2.90E−23



( japonica cultivar-



group)

368
G2146

Oryza sativa

gi5852091

0.015

368
G2146

Tulipa gesneriana

gi5923912

0.019

368
G2146

Petunia x hybrida

gi3127045

0.85

368
G2146

Gerbera hybrida

gi3650292

0.99

368
G2146

Oryza rufipogon

gi1086538

1

368
G2146

Sorghum bicolor

gi5276671

1

370
G2184

Petunia x hybrida

AF509870

1.00E−113

370
02184

Brassica napus

CD836672

4.00E−92

370
G2184

Lactuca sativa

BQ864249

3.00E−77

370
G2184

Solanum tuberosum

BG350410

9.00E−75

370
02184

Populus tremula x

BU863110

2.00E−71




Populus tremuloides


370
G2184

Brassica oleracea

BH526845

1.00E−65

370
G2184

Medicago

AW736414

1.00E−63




truncatula


370
G2184

Citrus sinensis

CB293271

3.00E−62

370
G2184

Oryza sativa

AK099237

2.00E−57



( japonica cultivar-



group)

370
G2184
Oryza sativa ( indica
CB633792

2.00E−57



cultivar-group)

370
G2184

Petunia x hybrida

gi21105742

1.10E−106

370
G2184

Oryza sativa

gi27452910

7.00E−58



( japonica cultivar-



group)

370
G2184

Medicago

gi7716952

4.60E−52




truncatula


370
G2184

Oryza sativa

gi6730946

8.30E−47

370
G2184

Brassica napus

gi31322578

2.20E−39

370
G2184

Lycopersicon

gi6175246

3.60E−39




esculentum


370
G2184

Phaseolus vulgaris

gi15148914

1.20E−38

370
G2184

Glycine max

gi22597158

3.30E−38

37G
G2184
Triticum sp.
gi4218537

2.90E−37

370
G2184

Triticum

gi6732160

2.90E−37




monococcum


371
G2207

Oryza sativa

Os_S17837
1605

371
G2207

Oryza sativa

Os_S6232
1606

371
G2207

Glycine max

Gma_S5129383
1667

371
G2207

Lycopersicon

SGN-UNIGENE-50991
2052




esculentum


371
G2207

Lycopersicon

SGN-UNIGENE-
2053




esculentum

SINGLET-399437

372
G2207

Oryza sativa

AK100046

1.00E−172



( japonica cultivar-



group)

372
G2207

Oryza sativa

AX654056

1.00E−168

372
G2207

Lotus japonicus

LJA239041

1.00E−148

372
G2207

Pisum sativum

PSA493066

1.00E−130

372
G2207

Brassica oleracea

BZ078380

1.00E−123

372
G2207
Oryza sativa ( indica
AAAA01002068

2.00E−76



cultivar-group)

372
G2207

Brassica nigra

AY061812

7.00E−71

372
G2207

Zea mays

CC644684

6.00E−70

372
G2207

Gossypium

BF269998

4.00E−58




arboreum


372
G2207

Lycopersicon

BI931640

7.00E−55




esculentum


372
G2207

Oryza sativa

gi20503001

1.00E−166



( japonica cultivar-



group)

372
G2207

Lotus japonicus

gi6448579

3.90E−160

372
G2207

Pisum sativum

gi23504759

4.50E−124

372
G2207

Oryza sativa

gi7339715

1.00E−122

372
G2207

Chlamydomonas

gi2190980

4.70E−06




incerta


372
G2207

Chlamydomonas

gi1928929

0.00049




reinhardtii


372
G2207

Bromheadia

gi2108256

0.55




finlaysoniana


372
G2207

Lycopersicon

gi100214

0.73




esculentum


372
G2207

Nicotiana tabacum

gi322758

0.81

372
G2207
Oryza sativa ( indica
gi2407271

0.96



cultivar-group)

374
G2213

Brassica oleracea

BH427528

2.00E−19

374
G2213

Gossypium

AI726978

2.00E−18




hirsutum


374
G2213

Zea mays

AY106664

1.00E−16

374
G2213

Glycine max

BE821577

1.00E−15

374
G2213

Triticum

BQ802007

2.00E−15




monococcum


374
G2213

Oryza sativa

AP004258

7.00E−15



( japonica cultivar-



group)

374
G2213
Oryza sativa ( indica
AAAA01008029

7.00E−15



cultivar-group)

374
G2213

Oryza sativa

AP003933

7.00E−15

374
G2213

Chlamydomonas

BU648678

4.00E−12




reinhardtii


374
G2213

Hordeum vulgare

BQ459689

2.00E−11

374
G2213

Oryza sativa

gi20161719

3.80E−28



( japonica cultivar-



group)

374
G2213

Oryza sativa

gi7339715

1.60E−08

374
G2213

Chlamydomonas

gi1928929

6.90E−07




reinhardtii


374
G2213

Chlamydomonas

gi2190980

2.50E−06




incerta


374
G2213

Pisum sativum

gi23504757

9.10E−05

374
G2213

Lotus japonicus

gi6448579

0.00014

374
G2213

Cicer arietinum

gi6002283

0.26

374
G2213

Allium cepa

gi19979631

0.82

374
G2213

Brassica napus

gi2809204

0.82

376
G2226

Thellungiella

BM985667

3.00E−71




halophila


376
G2226

Brassica napus

CD834580

9.00E−70

376
G2226

Glycine max

CA784214

2.00E−52

376
G2226

Gossypium

BF278307

4.00E−47




arboreum


376
G2226

Gossypium

AI727363

3.00E−46




hirsutum


376
G2226

Medicago

BF648350

7.00E−46




truncatula


376
G2226

Lactuca sativa

BU010488

1.00E−37

376
G2226

Solanum tuberosum

BG599025

5.00E−37

376
G2226

Capsicum annuum

CA525749

2.00E−36

376
G2226

Hevea brasiliensis

CB376421

2.00E−35

376
G2226

Thellungiella

gi20340241

2.30E−69




halophila


376
G2226

Oryza sativa

gi32488512

4.90E−12



( japonica cultivar-



group)

376
G2226

Oryza sativa

gi6069662

2.70E−11

376
G2226

Cucumis melo

gi28558782

1.00E−08

376
G2226

Medicago sativa

gi23451086

1.20E−08

376
G2226

Glycine max

gi22597166

2.70E−08

376
G2226

Hordeum vulgare

gi2894379

5.80E−08

376
G2226

Pisum sativum

gi4240031

2.30E−07

376
G2226

Hordeum vulgare

gi20152976

2.60E−07



subsp. vulgare

376
G2226

Zea mays

gi21645888

2.80E−07

378
G2227

Brassica oleracea

BZ520300

1.00E−123

378
G2227

Medicago

AC144609

2.00E−87




truncatula


378
G2227

Oryza sativa

AC079022

3.00E−81

378
G2227

Oryza sativa

AK067221

7.00E−69



( japonica cultivar-



group)

378
G2227
Oryza sativa ( indica
AAAA01002581

7.00E−69



cultivar-group)

378
G2227

Lycopersicon

AW035988

1.00E−67




esculentum


378
G2227

Lactuca sativa

BQ858069

6.00E−67

378
G2227

Vitis aestivalis

CB288974

9.00E−66

378
G2227

Glycine max

BM188705

5.00E−62

378
G2227

Zea mays

CG429342

1.00E−60

378
G2227

Oryza sativa

gi14719329

1.20E−79

378
G2227

Oryza sativa

gi29893617

6.70E−63



( japonica cultivar-



group)

378
G2227

Cicer arietinum

gi4651204

5.10E−21

378
G2227

Tulipa gesneriana

gi23386073

6.30E−19

378
G2227

Cucumis melo

gi28558782

8.40E−10

378
G2227

Hordeum vulgare

gi20152976

1.40E−07



subsp. vulgare

378
G2227

Thellungiella

gi20340241

9.80E−07




halophila


378
G2227

Glycine max

gi22597166

3.70E−06

378
G2227
Oryza sativa ( indica
gi29164825

8.60E−06



cultivar-group)

378
G2227

Medicago sativa

gi23451086

1.70E−05

379
G2239

Glycine max

GLYMA-28NOV01-
1125




CLUSTER12957_1

379
G2239

Glycine max

GLYMA-28NOV01-
1126




CLUSTER12957_2

379
G2239

Glycine max

GLYMA-28NOV01-
1127




CLUSTER12957_5

379
G2239

Glycine max

GLYMA-28NOV01-
1128




CLUSTER135837_1

379
G2239

Glycine max

uC-
1129




mf1LIB3275P131g04a1

379
G2239

Oryza sativa

ORYSA-22JAN02-
1130




CLUSTER139279_1

379
G2239

Oryza sativa

OSC100308.C1.p4.fg
1131

379
G2239

Oryza sativa

OSC101055.C1.p3.fg
1132

379
G2239

Oryza sativa

OSC102053.C1.p4.fg
1133

379
G2239

Oryza sativa

OSC20507.C1.p2.fg
1134

379
G2239

Zea mays

LIB4828-050-R1-N1-D2
1135

379
G2239

Zea mays

ZEAMA-08NOV01-
1136




CLUSTER495748_1

380
G2239

Brassica oleracea

BH582110

1.00E−120

380
G2239

Cucumis melo

AF499727

3.00E−73

380
G2239

Medicago

AC136503

4.00E−71




truncatula


380
G2239

Poncirus trifoliata

CD576402

8.00E−57

380
G2239

Citrus sinensis

CB290516

1.00E−56

380
G2239
Oryza sativa ( indica
AAAA01004423

4.00E−55



cultivar-group)

380
G2239

Oryza sativa

AP005399

4.00E−55



( japonica cultivar-



group)

380
G2239

Oryza sativa

AX653298

5.00E−51

380
G2239

Gossypium

AI730749

6.00E−51




hirsutum


380
G2239

Capsicum annuum

BM063816

1.00E−49

380
G2239

Cucumis melo

gi28558782

3.30E−70

380
G2239

Oryza sativa

gi21740711

2.10E−38

380
G2239

Oryza sativa

gi24756877

4.00E−33



( japonica cultivar-



group)

380
G2239

Hordeum vulgare

gi20152976

3.50E−21



subsp. vulgare

380
G2239

Nicotiana tabacum

gi12003386

2.20E−20

380
G2239

Medicago sativa

gi23451086

2.20E−17

380
G2239

Zea mays

gi21645888

1.20E−16

380
G2239

Hordeum vulgare

gi2894379

3.20E−16

380
G2239

Solanum tuberosum

gi24745601

4.70E−16

380
G2239

Thellungiella

gi20340241

4.30E−10




halophila


382
G2251

Brassica rapa

BG543052

2.00E−67



subsp. pekinensis

382
G2251

Populus tremula

BU893088

4.00E−48

382
G2251

Populus tremula x

BU894285

3.00E−47




Populus tremuloides


382
G2251

Glycine max

BE440750

5.00E−45

382
G2251

Gossypium

AI729600

2.00E−44




hirsutum


382
G2251

Lycopersicon

AW034559

8.00E−44




esculentum


382
G2251

Capsicum annuum

CA847343

1.00E−43

382
G2251

Lactuca sativa

BQ849490

2.00E−43

382
G2251

Brassica oleracea

BZ013045

4.00E−43

382
G2251

Ipomoea nil

BJ572086

3.00E−42

382
G2251

Oryza sativa

gi32488512

3.50E−29



( japonica cultivar-



group)

382
G2251

Oryza sativa

gi6069662

3.50E−18

382
G2251

Thellungiella

gi20340241

6.20E−12




halophila


382
G2251

Medicago sativa

gi23451086

7.00E−12

382
G2251

Cucumis melo

gi28558782

3.00E−10

382
G2251

Hordeum vulgare

gi20152976

3.10E−10



subsp. vulgare

382
G2251

Zea mays

gi21645888

3.70E−08

382
G2251

Glycine max

gi1076498

1.00E−07

382
G2251

Hordeum vulgare

gi2894379

8.20E−07

382
G2251

Nicotiana tabacum

gi12003386

9.40E−07

384
G2269

Brassica oleracea

BH433947

7.00E−72

384
G2269

Brassica napus

CD838796

1.00E−67

384
G2269

Gossypium

AI727683

8.00E−60




hirsutum


384
G2269

Glycine max

CA939042

8.00E−60

384
G2269

Gossypium

BG444995

1.00E−56




arboreum


384
G2269

Zea mays

CC694850

2.00E−47

384
G2269

Oryza sativa

AG109596

4.00E−47



( japonica cultivar-



group)

384
G2269

Oryza sativa

AC108499

4.00E−47

384
G2269
Oryza sativa (indica
AAAA01000476

7.00E−45



cultivar-group)

384
G2269

Ipomoea nil

BJ568099

1.00E−42

384
G2269

Oryza sativa

gi20160500

2.30E−44



( japonica cultivar-



group)

384
G2269

Gossypium

gi30983938

3.00E−21




barbadense


384
G2269

Oryza sativa

gi5091511

9.50E−16

384
G2269

Nicotiana tabacum

gi12003386

1.10E−12

384
G2269

Cucumis melo

gi28558782

3.20E−12

384
G2269

Hordeum vulgare

gi2894379

4.10E−12

384
G2269

Hordeum vulgare

gi20152976

6.00E−12



subsp. vulgare

384
G2269

Medicago sativa

gi23451086

2.00E−11

384
G2269

Zea mays

gi21645888

4.40E−09

384
G2269

Cicer arietinum

gi4651204

6.60E−09

386
G2298

Brassica oleracea

BH498020

2.00E−69

386
G2298

Beta vulgaris

BQ487577

9.00E−35

386
G2298

Populus tremula x

BU861348

2.00E−34




Populus tremuloides


386
G2298

Lycopersicon

AI489478

1.00E−33




esculentum


386
G2298

Glycine max

BE807652

1.00E−33

386
G2298

Lotus japonicus

AV424732

8.00E−33

386
G2298

Phaseolus vulgaris

BQ481785

1.00E−32

386
G2298

Solanum tuberosum

BI920063

1.00E−32

386
G2298

Populus

CA826218

4.00E−32



balsamifera subsp.




trichocarpa x





Populus deltoides


386
G2298

Brassica napus

CD819945

1.00E−30

386
G2298

Atriplex hortensis

gi8571476

1.20E−21

386
G2298

Oryza sativa

gi21742362

1.80E−21



( japonica cultivar-



group)

386
G2298

Oryza sativa

gi14140155

6.40E−21

386
G2298

Zea mays

gi21908036

1.90E−19

386
G2298

Prunus armeniaca

gi3264767

2.30E−19

386
G2298

Lycopersicon

gi27436378

4.50E−18




esculentum


386
G2298

Glycine max

gi31324058

4.50E−18

386
G2298

Stylosanthes hamata

gi4099914

1.70E−17

386
G2298

Nicotiana tabacum

gi10798644

1.10E−16

386
G2298

Triticum aestivum

gi15488459

1.70E−16

388
G2311

Brassica napus

CD822731

1.00E−57

388
G2311

Gossypium

BE053309

2.00E−51




arboreum


388
G2311

Oryza sativa

OSA495797

1.00E−47



( japonica cultivar-



group)

388
G2311

Zea mays

AF461815

1.00E−45

388
G2311

Glycine max

BU761883

1.00E−44

388
G2311

Populus tremula x

BU827154

2.00E−42




Populus tremuloides


388
G2311

Petroselinum

PCU67132

1.00E−41




crispum


388
G2311

Medicago

AW329290

6.00E−41




truncatula


388
G2311

Ipomoea nil

BJ554376

3.00E−39

388
G2311

Triticum aestivum

CA502640

5.00E−38

388
G2311

Oryza sativa

gi20804653

4.00E−49



( japonica cultivar-



group)

388
G2311

Zea mays

gi18463961

2.90E−46

388
G2311

Petroselinum

gi2224897

2.00E−22




crispum


388
G2311

Fritillaria liliacea

gi15281594

8.70E−11

388
G2311

Triticum aestivum

gi2980891

2.30E−09

388
G2311

Euphorbia esula

gi6752901

5.50E−09

388
G2311

Vicia faba

gi30420970

2.60E−08

388
G2311

Fritillaria agrestis

gi2641211

3.00E−08

388
G2311

Pisum sativum

gi4106696

7.10E−08

388
G2311

Lathyrus aphaca

gi21465093

7.30E−08

389
G2317

Glycine max

GLYMA-28NOV01-
1137




CLUSTER2162_3

389
G2317

Glycine max

GLYMA-28NOV01-
1138




CLUSTER2162_4

389
G2317

Oryza sativa

Os_S96262
1607

389
G2317

Glycine max

Gma_S4915353
1668

389
G2317

Medicago

Mtr_S5363423
1709




truncatula


389
G2317

Hordeum vulgare

Hv_S60108
1741

389
G2317

Lycopersicon

Les_S5190181
1933




esculentum


390
G2317

Medicago

BG453991

5.00E−51




truncatula


390
G2317

Zinnia elegans

AU292197

2.00E−42

390
G2317

Triticum aestivum

BT009406

1.00E−41

390
G2317

Solanum tuberosum

BM110307

1.00E−41

390
G2317

Oryza sativa

AX658868

3.00E−41

390
G2317

Glycine max

BM093706

6.00E−41

390
G2317

Lycopersicon

AI774224

1.00E−39




esculentum


390
G2317

Hordeum vulgare

BJ472642

7.00E−39



subsp. vulgare

390
G2317

Oryza sativa

AK101209

4.00E−37



( japonica cultivar-



group)

390
G2317

Sorghum bicolor

CD219576

7.00E−37

390
G2317

Phaseolus vulgaris

gi21213868

2.60E−36

390
G2317

Oryza sativa

gi21742243

5.50E−36



( japonica cultivar-



group)

390
G2317

Oryza sativa

gi15528628

6.00E−23

390
G2317

Hordeum vulgare

gi12406993

1.10E−07

390
G2317

Hevea brasiliensis

gi12005328

4.90E−07

390
G2317

Antirrhinum majus

gi18874263

1.80E−06

390
G2317

Malus xiaojinensis

gi28629811

3.80E−06

390
G2317

Glycine max

gi19911577

3.90E−06

390
G2317

Zea mays

gi20067661

1.00E−05

390
G2317

Lycopersicon

gi6688529

2.60E−05




esculentum


391
G2319

Glycine max

GLYMA-28NOV01-
1139




CLUSTER33063_1

391
G2319

Glycine max

GLYMA-28NOV01-
1140




CLUSTER33063_2

391
G2319

Glycine max

GLYMA-28NOV01-
1141





CLUSTER33063_4

391
G2319

Glycine max

jC-gmst024169f06b1
1142

391
G2319

Lycopersicon

SGN-UNIGENE-
2054




esculentum

SINGLET-335798

392
G2319

Lycopersicon

BI921951

7.00E−93




esculentum


392
G2319

Triticum aestivum

BT008954

2.00E−71

392
G2319

Gossypium

BQ403368

4.00E−71




arboreum


392
G2319

Zea mays

AX756404

8.00E−70

392
G2319

Mesembryanthemum

GA840558

7.00E−69




crystallinum


392
G2319

Citrus sinensis

CB290239

2.00E−65

392
G2319

Populus tremula x

BU887003

1.00E−62




Populus tremuloides


392
G2319

Glycine max

CD487127

2.00E−58

392
G2319

Hordeum vulgare

AV909036

1.00E−56



subsp. vulgare

392
G2319

Medicago

BF005534

7.00E−55




truncatula


392
G2319

Oryza sativa

gi15528628

2.70E−38

392
G2319

Oryza sativa

gi21742243

1.80E−25



( japonica cultivar-



group)

392
G2319

Phaseolus vulgaris

gi21213868

5.00E−25

392
G2319

Hordeum vulgare

gi12406993

8.80E−08

392
G2319

Hevea brasiliensis

gi12005328

2.50E−06

392
G2319

Glycine max

gi19911577

3.30E−06

392
G2319

Lycopersicon

gi6688529

5.20E−06




esculentum


392
G2319

Solanum tuberosum

gi7705206

9.00E−06

392
G2319

Zea mays

gi20067661

1.90E−05

392
G2319

Malus xiaojinensis

gi28629811

2.00E−05

393
G2334

Lycopersicon

SGN-UNIGENE-57794
2055




esculentum


394
G2334

Brassica oleracea

BZ428330

5.00E−61

394
G2334

Medicago

AW981431

1.00E−30




truncatula


394
G2334

Glycine max

BI786182

3.00E−30

394
G2334

Solanum tuberosum

BE922572

7.00E−30

394
G2334

Oryza sativa

AK110934

7.00E−30



( japonica cultivar-



group)

394
G2334

Amborella

CD483211

3.00E−29




trichopoda


394
G2334

Lycopersicon

AW650563

4.00E−29




esculentum


394
G2334

Oryza sativa

AF201895

6.00E−29

394
G2334

Hordeum vulgare

CA029723

6.00E−29



subsp. vulgare

394
G2334

Zea mays

CA828910

2.00E−28

394
G2334

Oryza sativa

gi6573149

6.20E−37

394
G2334

Oryza sativa

gi24413958

1.80E−35



( japonica cultivar-



group)

394
G2334

Sorghum bicolor

gi18390099

6.00E−33

394
G2334

Solanum

gi32470646

5.90E−32




bulbocastanum


394
G2334

Nicotiana alata

gi1087017

0.79

394
G2334

Petunia x hybrida

gi14522848

0.94

394
G2334

Picea abies

gi10764150

0.98

394
G2334
Oryza sativa ( indica
gi4680183

1



cultivar-group)

394
G2334

Lycopersicon

gi1418988

1




esculentum


394
G2334

Pyrus pyrifolia

gi8698889

1

396
G2371

Brassica oleracea

BH518264

2.00E−83

396
G2371

Brassica napus

CD815763

8.00E−71

396
G2371

Capsicum annuum

BM063508

7.00E−50

396
G2371

Oryza sativa

AX072663

2.00E−45



( japonica cultivar-



group)

396
G2371

Solanum tuberosum

BQ115437

9.00E−45

396
G2371

Lycopersicon

BI921667

3.00E−44




esculentum


396
G2371
Oryza sativa ( indica
AAAA01005658

1.00E−43



cultivar-group)

396
G2371

Triticum aestivum

BE499367

3.00E−43

396
G2371

Glycine max

AW781777

4.00E−43

396
G2371

Lycopersicon

AW617994

6.00E−41




pennellii


396
G2371

Oryza sativa

gi21426118

2.20E−42



( japonica cultivar-



group)

396
G2371

Marchantia

gi25272004

7.20E−32




polymorpha


396
G2371

Oryza sativa

gi19352041

3.20E−07

396
G2371

Mangifera indica

gi31747324

5.40E−06

396
G2371

Prunus persica

gi27450533

7.40E−06

396
G2371

Eragrostis tef

gi17906977

9.80E−06

396
G2371

Hordeum vulgare

gi1730475

1.30E−05



subsp. vulgare

396
G2371

Phaseolus vulgaris

gi1046278

5.70E−05

396
G2371

Mesembryanthemum

gi3219155

0.00012




crystallinum


396
G2371

Zea mays

gi100922

0.00015

398
G2372

Oryza sativa

ABO71299

3.00E−97

398
G2372
Oryza sativa ( indica
AAAA01008877

3.00E−97



cultivar-group)

398
G2372

Oryza sativa

AK100322

2.00E−96



( japonica cultivar-



group)

398
G2372

Physcomitrella

AX288142

5.00E−93




patens


398
G2372

Brassica oleracea

BZ495025

1.00E−87

398
G2372

Lotus japonicus

AP004505

5.00E−77

398
G2372

Gossypium

BG443827

2.00E−68




arboreum


398
G2372

Zea mays

CC657791

2.00E−66

398
G2372

Vitis vinifera

GB979491

7.00E−59

398
G2372

Medicago

BG646821

3.00E−58




truncatula


398
G2372

Oryza sativa

gi13384374

3.10E−97



( japonica cultivar-



group)

398
G2372

Oryza sativa

gi19352051

1.50E−95

398
G2372

Prunus persica

gi27450533

1.60E−50

398
G2372
Oryza sativa ( indica
gi26251300

3.40E−46



cultivar-group)

398
G2372

Mangifera indica

gi30027167

1.10E−42

398
G2372

Marchantia

gi25272004

1.30E−10




polymorpha


398
G2372

Chamaecyparis

gi30421190

0.97



nootkatensis

400
G2375

Brassica oleracea

BH685171

2.00E−84

400
G2375

Medicago

BG648693

1.00E−56




truncatula


400
G2375
Oryza sativa ( indica
AAAA01004311

6.00E−42



cultivar-group)

400
G2375

Nicotiana tabacum

E64987

1.00E−28

400
G2375

Malus x domestica

AU301396

2.00E−28

400
G2375

Pinus taeda

BG040491

7.00E−26

400
G2375

Zea mays

AY109868

3.00E−25

400
G2375

Lycopersicon

BG123901

1.00E−24




esculentum


400
G2375

Solanum tuberosum

BQ118023

1.00E−23

400
G2375

Oryza sativa

OSJN00077

7.00E−23

400
G2375

Oryza sativa

gi21740920

4.60E−48



( japonica cultivar-



group)

400
G2375

Oryza sativa

gi20249

4.90E−15

400
G2375

Nicotiana tabacum

gi170271

2.20E−14

400
G2375
Oryza sativa ( indica
gi27368895

3.40E−12



cultivar-group)

400
G2375

Pisum sativum

gi13646986

1.20E−11

400
G2375

Glycine max

gi18182311

5.40E−07

400
G2375

Cucurbita maxima

gi17221648

3.70E−06

400
G2375

Lycopersicon

gi9858781

0.00023




esculentum


400
G2375

Daucus carota

gi2190187

0.00051

400
G2375

Fagopyrum

gi31088123

0.007




urophyllum


401
G2382

Medicago

Mtr_S7091755
1710




truncatula


401
G2382

Triticum aestivum

Ta_S282850
1902

401
G2382

Lycopersicon

SGN-UNIGENE-47842
2056




esculentum


401
G2382

Lycopersicon

SGN-UNIGENE-57633
2057




esculentum


402
G2382

Brassica oleracea

BH542030

1.00E−104

402
G2382

Brassica napus

CD814588

2.00E−63

402
G2382

Vitis vinifera

CB347529

2.00E−57

402
G2382

Ipomoea nil

BJ575415

4.00E−45

402
G2382

Lycopersicon

AW441337

2.00E−40




esculentum


402
G2382

Medicago

CA921647

6.00E−40




truncatula


402
G2382

Lactuca sativa

BU006964

3.00E−39

402
G2382

Vitis aestivalis

CB289221

1.00E−34

402
G2382

Oryza sativa

AP003453

9.00E−34

402
G2382
Oryza sativa ( indica
AAAA01000618

8.00E−32



cultivar-group)

402
G2382

Oryza sativa

gi15624003

4.00E−33



( japonica cultivar-



group)

402
G2382

Nicotiana tabacum

gi18149189

6.90E−23

402
G2382

Oryza sativa

gi12597883

3.80E−08

402
G2382

Chloroplast

gi16551225

8.60E−07




Panicum





koolauense


402
G2382

Chloroplast

gi16551261

5.90E−05




Paspalum





fimbriatum


402
G2382

Chloroplast

gi16551247

0.00022




Mesosetum chaseae


402
G2382

Chloroplast

gi16551309

0.00027




Thrasya petrosa


402
G2382

Chloroplast

gi16551305

0.00072




Tatianyx arnacites


402
G2382

Chloroplast

gi16551207

0.00078




Altoparadisium





chapadense


402
G2382

Chloroplast

gi16551307

0.0011




Thrasya glaziovii


404
G2394

Oryza sativa

AK071804

1.00E−108



( japonica cultivar-



group)

404
G2394

Zea mays

BG837939

2.00E−85

404
G2394

Oryza sativa

AX699700

3.00E−72

404
02394

Triticum aestivum

BJ319065

7.00E−72

404
G2394
Oryza sativa ( indica
CB634885

3.00E−69



cultivar-group)

404
G2394

Lactuca sativa

BQ852089

4.00E−69

404
G2394

Lycopersicon

BI921710

1.00E−67




esculentum


404
G2394

Hordeum vulgare

AL505242

9.00E−64



subsp. vulgare

404
G2394

Hordeum vulgare

BU991885

3.00E−60

404
G2394

Solanum tuberosum

BQ512426

3.00E−57

404
G2394

Oryza sativa

gi15289774

1.50E−74



( japonica cultivar-



group)

404
G2394

Phacelia

gi5002214

1.50E−24




tanacetifolia


404
G2394

Oryza sativa

gi14164470

1.40E−13

404
G2394

Hordeum vulgare

gi20152976

1.80E−12



subsp. vulgare

404
G2394

Cicer arietinum

gi10334499

6.90E−12

404
G2394

Cucumis melo

gi17016985

8.10E−12

404
G2394

Thellungiella

gi20340241

7.80E−11




halophila


404
G2394

Nicotiana tabacum

gi12003386

8.80E−10

404
G2394

Zea mays

gi21645888

1.10E−09

404
G2394

Hordeum vulgare

gi2894379

2.30E−09

406
G2404

Brassica oleracea

BH418639

3.00E−61

406
G2404

Descurainia sophia

BU238407

1.00E−47

406
G2404

Medicago

BQ148493

3.00E−45




truncatula


406
G2404

Brassica napus

CD815525

3.00E−41

406
G2404

Vitis vinifera

CB983093

1.00E−39

406
G2404

Glycine max

BQ741279

6.00E−39

406
G2404

Nicotiana tabacum

BP129655

3.00E−36

406
G2404

Solanum tuberosum

BQ507078

5.00E−36

406
G2404

Populus tremula x

BI131425

5.00E−33




Populus tremuloides


406
G2404

Oryza sativa

AU101377

2.00E−31

406
G2404

Oryza sativa

gi18565429

3.90E−41



( japonica cultivar-



group)

406
G2404

Pisum sativum

gi4240031

8.40E−29

406
G2404

Lotus japonicus

gi1086225

3.10E−27

406
G2404

Oryza sativa

gi8570055

5.10E−26

406
G2404

Glycine max

gi1076498

1.00E−25

406
G2404

Cicer arietinum

gi10334499

1.80E−14

406
G2404

Thellungiella

gi20340241

9.50E−09




halophila


406
G2404

Cucumis melo

gi17016985

1.20E−07

406
G2404
Oryza sativa ( indica
gi29164825

1.50E−07



cultivar-group)

406
G2404

Hordeum vulgare

gi2894379

4.80E−06

407
G2432

Glycine max

GLYMA-28NOV01-
1143




CLUSTER68229_1

407
G2432

Glycine max

GLYMA-28NOV01-
1144




CLUSTER68229_2

407
G2432

Oryza sativa

OSC2225.C1.p2.fg
1145

407
G2432

Oryza sativa

Os_S60255
1608

407
G2432

Lycopersicon

SGN-UNIGENE-
2058




esculentum

SINGLET-18277

408
G2432

Brassica oleracea

BH959523

7.00E−85

408
G2432

Brassica napus

CD824503

6.00E−76

408
G2432

Populus

BU868493

7.00E−47



balsamifera subsp.




trichocarpa


408
G2432

Lycopersicon

AW648389

7.00E−39




esculentum


408
G2432

Vitis vinifera

CA810654

1.00E−36

408
G2432

Medicago

BE323614

2.00E−35




truncatula


408
G2432

Glycine max

BE474759

3.00E−31

408
G2432

Hordeum vulgare

HVU312330

1.00E−30



subsp. vulgare

408
G2432

Beta vulgaris

BQ584245

2.00E−29

408
G2432
Oryza sativa ( indica
CB622873

3.00E−29



cultivar-group)

408
G2432

Oryza sativa

gi15451553

3.40E−33

408
G2432

Oryza sativa

gi31431991

3.40E−33



( japonica cultivar-



group)

408
G2432

Hordeum vulgare

gi21538791

3.70E−30



subsp. vulgare

408
G2432

Cucurbita maxima

gi1669341

1.10E−28

408
G2432

Dendrobium grex

gi3929325

6.60E−24



Madame Thong-In

408
G2432

Hordeum vulgare

gi3777436

1.20E−22

408
G2432

Zea mays

gi1346559

2.00E−22

408
G2432

Pisum sativum

gi6092016

5.40E−22

408
G2432

Nicotiana tabacum

gi3341468

1.10E−21

408
G2432

Triticum aestivum

gi3790264

1.10E−21

410
G2443

Brassica napus

A50832

1.00E−148

410
G2443

Brassica nigra

AF269127

1.00E−131

410
G2443

Brassica oleracea

BH650127

1.00E−93

410
G2443

Ipomoea nil

AF300700

2.00E−86

410
G2443

Oryza sativa

AB041838

1.00E−65

410
G2443

Malus domestica

AF052585

7.00E−62

410
G2443

Raphanus sativus

AF052690

2.00E−59

410
G2443

Pinus radiata

AF001136

2.00E−58

410
G2443

Medicago

AC127169

7.00E−57




truncatula


410
G2443

Mesembryanthemum

BM300894

2.00E−54




crystallinum


410
G2443

Brassica napus

gi2303681

1.80E−140

410
G2443

Brassica nigra

gi11037308

9.00E−139

410
G2443

Oryza sativa

gi11094203

1.90E−65

410
G2443

Oryza sativa

gi23589949

1.90E−65



( japonica cultivar-



group)

410
G2443

Hordeum vulgare

gi21667475

1.10E−60

410
G2443

Malus x domestica

gi4091804

2.10E−59

410
G2443

Raphanus sativus

gi3341723

1.80E−56

410
G2443

Ipomoea nil

gi10946337

1.90E−51

410
G2443

Hordeum vulgare

gi21655154

6.60E−51



subsp. vulgare

410
G2443

Pinus radiata

gi4557093

2.90E−39

411
G2453

Glycine max

BF070025.1
1146

411
G2453

Oryza sativa

Os_S112425
1609

411
G2453

Zea mays

Zm_S11447234
1813

412
G2453

Chrysanthemum x

AY173066

1.00E−33




morifolium


412
G2453

Lactuca sativa

BQ995044

3.00E−33

412
G2453

Glycine max

CA935182

1.00E−32

412
G2453

Hordeum vulgare

BI949633

2.00E−32

412
G2453

Antirrhinum majus

AJ559762

6.00E−32

412
G2453

Lycopersicon

BI931349

1.00E−31




esculentum


412
G2453

Solanum tuberosum

BQ117114

1.00E−31

412
G2453

Mentha x piperita

AW255624

2.00E−31

412
G2453

Oryza sativa

AU094883

2.00E−31



( japonica cultivar-



group)

412
G2453

Hordeum vulgare

BU968850

2.00E−31



subsp. vulgare

412
G2453

Chrysanthemum x

gi27804377

1.70E−34




morifolium


412
G2453

Zea mays

gi32330681

2.00E−34

412
G2453

Oryza sativa

gi22267600

1.40E−33



( japonica cultivar-



group)

412
G2453

Oryza sativa

gi11280864

3.60E−32

412
G2453

Daucus carota

gi3551257

0.81

412
G2453

Vicia faba

gi2104679

0.82

412
G2453

Brassica rapa

gi29123372

0.9



subsp. pekinensis

412
G2453

Nicotiana tabacum

gi2196548

0.97

412
G2453
Oryza sativa ( indica
gi23345287

1



cultivar-group)

412
G2453
Narcissus
gi18419623

1




pseudonarcissus


414
G2455

Brassica napus

CD830903

3.00E−63

414
G2455

Lycopersicon

BM410884

3.00E−58




esculentum


414
G2455

Glycine max

BU579031

2.00E−56

414
G2455

Populus

BU871780

9.00E−56



balsamifera subsp.




trichocarpa


414
G2455

Populus tremula x

BU864185

2.00E−53




Populus tremuloides


414
G2455

Prunus persica

BU044242

3.00E−50

414
G2455

Cycas rumphii

CB093475

1.00E−48

414
G2455

Nuphar advena

CD472928

9.00E−48

414
G2455

Antirrhinum majus

AJ559762

1.00E−46

414
G2455

Triticum aestivum

BJ264502

5.00E−44

414
G2455

Chrysanthemum x

gi27804377

5.60E−43




morifolium


414
G2455

Zea mays

gi32330677

6.40E−43

414
G2455

Oryza sativa

gi11280864

7.10E−43

414
G2455

Oryza sativa

gi33146736

9.10E−43



( japonica cultivar-



group)

414
G2455

Glycine max

gi5019730

0.16

414
G2455

Brassica rapa

gi29123372

0.2



subsp. pekinensis

414
G2455
Oryza sativa ( indica
gi2570503

0.69



cultivar-group)

416
G2456

Glycine max

CA800830

3.00E−67

416
G2456

Chrysanthemum x

AY173066

5.00E−63




morifolium


416
G2456

Lycopersicon

AW623191

6.00E−62




esculentum


416
G2456

Populus tremula x

BU830431

5.00E−61




Populus tremuloides


416
G2456

Solanum tuberosum

BQ117114

2.00E−58

416
G2456

Zea mays

AY313904

6.00E−58

416
G2456

Lactuca sativa

BQ995044

1.00E−57

416
G2456

Oryza sativa

AK106784

2.00E−57



( japonica cultivar-



group)

416
G2456

Nuphar advena

CD475930

1.00E−52

416
G2456

Populus tremuloides

CA931450

8.00E−50

416
G2456

Oryza sativa

gi22267600

1.20E−61



( japonica cultivar-



group)

416
G2456

Zea mays

gi32330681

8.50E−61

416
G2456

Chrysanthemum x

gi27804377

8.80E−61




morifolium


416
G2456

Oryza sativa

gi11280863

7.90E−37

416
G2456

Brassica rapa

gi29123372

0.027



subsp. pekinensis

416
G2456

Triticum aestivum

gi32400832

0.037

416
G2456

Solanum tuberosum

gi9954112

0.038

416
G2456

Phaseolus vulgaris

gi457750

0.04

416
G2456

Volvox carteri f.

gi6523547

0.22




nagariensis


416
G2456

Capsella rubella

gi8919877

0.45

417
G2457

Glycine max

GLYMA-28NOV01-
1147




CLUSTER62346_1

417
G2457

Oryza sativa

ORYSA-22JAN02-
1148




CLUSTER47850_1

417
G2457

Oryza sativa

ORYSA-22JAN02-
1149




CLUSTER504_1

417
G2457

Oryza sativa

OSC102186.C1.p13.fg
1150

417
G2457

Oryza sativa

OSC29392.C1.p1.fg
1151

417
G2457

Oryza sativa

OSC7136.C1.p1.fg
1152

417
G2457

Zea mays

ZEAMA-08NOV01-
1153




CLUSTER603_35

417
G2457

Zea mays

ZEAMA-08NOV01-
1154




CLUSTER779_19

417
G2457

Zea mays

ZEAMA-08NOV01-
1155




CLUSTER779_20

417
G2457

Zea mays

ZEAMA-08NOV01-
1156




CLUSTER779_24

417
G2457

Zea mays

ZEAMA-08NOV01-
1157




CLUSTER779_9

417
G2457

Hordeum vulgare

Hv_S18520
1742

417
G2457

Zea mays

Zm_S11528333
1814

417
G2457

Triticum aestivum

Ta_S241823
1903

417
G2457

Triticum aestivum

Ta_S417975
1904

418
G2457

Antirrhinum majus

AJ559642

6.00E−55

418
G2457

Lycopersicon

AI483816

7.00E−53




esculentum


418
G2457

Hedyotis

CB088054

9.00E−53




centranthoides


418
G2457

Zea mays

AY104291

1.00E−41

418
G2457

Triticum aestivum

BT008985

5.00E−41

418
G2457

Hordeum vulgare

BQ462679

3.00E−40



subsp. vulgare

418
G2457

Populus

BU880953

5.00E−30



balsamifera subsp.




trichocarpa


418
G2457

Oryza sativa

AK104918

5.00E−30



( japonica cultivar-



group)

418
G2457

Populus tremula x

BU864185

5.00E−30




Populus tremuloides


418
G2457

Prunus persica

BU043103

2.00E−29

418
G2457

Zea mays

gi32330677

8.50E−32

418
G2457

Oryza sativa

gi22267600

9.50E−31



( japonica cultivar-



group)

418
G2457

Oryza sativa

gi11280863

4.70E−30

418
G2457

Chrysanthemum x

gi27804377

1.70E−18




morifolium


418
G2457

Brassica rapa

gi29123372

1.70E−16



subsp. pekinensis

418
G2457

Sesbania rostrata

gi169880

0.3

418
G2457

Lycopersicon

gi4467884

0.45




esculentum


418
G2457

Dianthus

gi2406586

0.63




caryophyllus


418
G2457

Vicia faba

gi4468042

0.81

418
G2457

Pisum sativum

gi100057

0.96

419
G2459

Glycine max

GLYMA-28NOV01-
1147




CLUSTER62346_1

419
G2459

Oryza sativa

LIB4370-016-R1-K1-B3
1158

419
G2459

Oryza sativa

ORYSA-22JAN02-
1148




CLUSTER47850_1

419
G2459

Oryza sativa

ORYSA-22JAN02-
1149




CLUSTER504_1

419
G2459

Oryza sativa

OSC102186.C1.p13.fg
1150

419
G2459

Oryza sativa

OSC29392.C1.pl.fg
1151

419
G2459

Oryza sativa

OSC7136.C1.p1.fg
1152

419
G2459

Oryza sativa

jC-osflLIB3479001b04b1
1159

419
G2459

Zea mays

ZEAMA-08NOV01-
1160




CLUSTER603_34

419
G2459

Zea mays

ZEAMA-08NOV01-
1153




CLUSTER603_35

419
G2459

Triticum aestivum

Ta_S241823
1903

419
G2459

Lycopersicon

SGN-UNIGENE-
2059




esculentum

SINGLET-70658

420
G2459

Brassica napus

CD830187

4.00E−84

420
G2459

Glycine max

CA800542

4.00E−68

420
G2459

Lycopersicon

AI484101

2.00E−66




esculentum


420
G2459

Antirrhinum majus

AJ559762

6.00E−61

420
G2459

Mentha x piperita

AW255624

4.00E−58

420
G2459

Solanum tuberosum

BQ516639

2.00E−56

420
G2459

Ipomoea nil

BJ575190

6.00E−51

420
G2459

Populus

BU880953

2.00E−49



balsamifera subsp.




trichocarpa


420
G2459

Populus tremula x

BU864185

2.00E−48




Populus tremuloides


420
G2459

Nuphar advena

CD474192

1.00E−47

420
G2459

Oryza sativa

gi11280864

1.30E−45

420
G2459

Zea mays

gi32330677

7.30E−44

420
G2459

Oryza sativa

gi22267600

1.20E−43



( japonica cultivar-



group)

420
G2459

Chrysanthemum x

gi27804377

1.20E−26




morifolium


420
G2459

Brassica rapa

gi29123372

0.013



subsp. pekinensis

420
G2459

Nicotiana tabacum

gi4206787

0.68

420
G2459

Narcissus

gi18419623

1




pseudonarcissus


420
G2459

Pinus koraiensis

gi29469765

1

422
G2467

Brassica oleracea

BH657157

7.00E−72

422
G2467

Citrus sinensis

CB291749

8.00E−63

422
G2467

Oryza sativa

AX653477

1.00E−59

422
G2467

Oryza sativa

AK068660

2.00E−59



( japonica cultivar-



group)

422
G2467

Hordeum vulgare

AV833112

5.00E−51



subsp. vulgare

422
G2467

Lycopersicon

LPHSF30

3.00E−50




peruvianum


422
G2467

Medicago

BE319312

2.00E−49




truncatula


422
G2467
Oryza sativa ( indica
AAAA01016817

5.00E−49



cultivar-group)

422
G2467

Zea mays

CC610706

5.00E−49

422
G2467

Helianthus annuus

BQ916240

9.00E−48

422
G2467

Oryza sativa

gi28971956

2.20E−58



( japonica cultivar-



group)

422
G2467

Lycopersicon

gi100265

5.70E−50




peruvianum


422
G2467

Helianthus annuus

gi25052685

7.90E−44

422
G2467

Glycine max

gi2129828

9.10E−43

422
G2467

Oryza sativa

gi16580739

1.70E−42

422
G2467

Phaseolus

gi16118447

6.20E−37




acutifolius


422
G2467

Nicotiana tabacum

gi5821138

1.00E−36

422
G2467

Lycopersicon

gi100225

1.30E−36




esculentum


422
G2467

Medicago sativa

gi20162459

1.10E−35

422
G2467

Pisum sativum

gi3550552

4.50E−34

424
G2492

Oryza sativa

AK073195

1.00E−126



( japonica cultivar-



group)

424
G2492

Pisum sativum

PSPD3BIPR

3.00E−72

424
G2492

Vicia sativa

VSENBP1GN

2.00E−71

424
G2492

Lactuca sativa

BU008717

4.00E−69

424
G2492

Vitis vinifera

CB348303

8.00E−67

424
G2492

Mesembryanthemum

CA838861

9.00E−65




crystallinum


424
G2492

Zea mays

AY106688

2.00E−64

424
G2492

Brassica oleracea

BH582293

1.00E−60

424
G2492

Triphysaria

BM357136

1.00E−60




versicolor


424
G2492

Ipomoea nil

BJ577989

6.00E−59

424
G2492

Medicago

gi11358945

2.50E−153




truncatula


424
G2492

Pisum sativum

gi2213540

3.00E−151

424
G2492

Vicia sativa

gi1360637

1.30E−150

424
G2492

Nicotiana tabacum

gi8096269

7.20E−10

424
G2492

Brassica oleracea

gi15054376

9.90E−08

424
G2492

Oryza sativa

gi6942229

6.40E−06

424
G2492

Zea mays

gi4138732

3.90E−05

424
G2492

Saccharum hybrid

gi21902142

0.00016



cultivar CP72-2086

424
G2492

Oryza sativa

gi29367369

0.00062



( japonica cultivar-



group)

424
G2492

Saccharum hybrid

gi18873729

0.0016



cultivar CP65-357

425
G2505

Glycine max

GLYMA-28NOV01-
1161




CLUSTER81568_1

425
G2505

Zea mays

LIB5116-010-A1-PF1-D6
1162

426
G2505

Populus

BU879250

7.00E−72



balsamifera subsp.




trichocarpa


426
G2505

Medicago

BF645892

5.00E−70




truncatula


426
G2505

Sorghum bicolor

CD230282

6.00E−68

426
G2505

Oryza sativa

AK109860

2.00E−67



( japonica cultivar-



group)

426
G2505

Oryza sativa

AB028186

6.00E−66

426
G2505

Populus

CA826386

2.00E−63



balsamifera subsp.




trichocarpa x





Populus deltoides


426
G2505

Lycopersicon

BF098091

5.00E−62




esculentum


426
G2505

Triticum aestivum

BQ483881

7.00E−62

426
G2505

Hordeum vulgare

BE060921

4.00E−61

426
G2505
Oryza sativa ( indica
AAAA01001925

1.00E−56



cultivar-group)

426
G2505

Oryza sativa

gi11875152

1.70E−66

426
G2505

Oryza sativa

gi28190666

1.70E−66



( japonica cultivar-



group)

426
G2505

Petunia x hybrida

gi1279640

5.80E−48

426
G2505

Glycine max

gi22597158

7.50E−48

426
G2505

Zea mays

gi32527660

4.50E−46

426
G2505

Phaseolus vulgaris

gi15148914

6.00E−46

426
G2505
Triticum sp.
gi4218537

5.40E−45

426
G2505

Triticum

gi6732156

5.40E−45




monococcum


426
G2505

Lycopersicon

gi6175246

1.30E−43




esculentum


426
G2505

Brassica napus

gi31322582

3.10E−42

428
G2515

Betula pendula

BPMADS5GN

1.00E−59

428
G2515

Vitis vinifera

AY275713

1.00E−56

428
G2515

Petunia x hybrida

AF176782

2.00E−55

428
G2515
Petunia sp.
A81451

2.00E−55

428
G2515

Chrysanthemum x

AY173055

2.00E−55




morifolium


428
G2515

Nicotiana tabacum

AF385746

4.00E−55

428
G2515

Capsicum annuum

AF130118

4.00E−55

428
G2515

Eucalyptus globulus

AF306349

1.00E−54

428
G2515

Antirrhinum majus

AY040247

2.00E−54

428
G2515

Daucus carota

DCA271147

3.00E−54

428
G2515

Betula pendula

gi1483232

6.40E−58

428
G2515

Vitis vinifera

gi30526323

8.40E−56

428
G2515

Chrysanthemum x

gi27804357

2.60E−54




morifolium


428
G2515

Capsicum annuum

gi14518447

2.60E−54

428
G2515

Petunia x hybrida

gi6634708

3.30E−54

428
G2515
Petunia sp.
gi6731756

3.30E−54

428
G2515

Nicotiana tabacum

gi27373049

5.30E−54

428
G2515

Eucalyptus globulus

gi11120557

6.80E−54

428
G2515

Nicotiana sylvestris

gi5070142

2.30E−53

428
G2515

Daucus carota

gi22091473

2.90E−53

430
G2525

Oryza sativa

AC145379

3.00E−86



( japonica cultivar-



group)

430
G2525

Solanum tuberosum

BQ115041

6.00E−76

430
G2525

Suaeda maritima

BE231373

2.00E−69



subsp. salsa

430
G2525

Brassica oleracea

BH432132

2.00E−67

430
G2525

Hordeum vulgare

CB878456

3.00E−65



subsp. vulgare

430
G2525

Lactuca sativa

BU013946

1.00E−63

430
G2525

Medicago

BE325206

1.00E−62




truncatula


430
G2525

Gossypium

AI728383

6.00E−60




hirsutum


430
G2525

Zea mays

CC641341

2.00E−58

430
G2525
Oryza sativa ( indica
AAAA01009282

5.00E−58



cultivar-group)

430
G2525

Oryza sativa

gi18873845

1.20E−44

430
G2525

Oryza sativa

gi31433498

1.20E−44



( japonica cultivar-



group)

430
G2525

Spermatozopsis

gi3417441

0.14




similis


430
G2525

Nicotiana tabacum

gi237857

0.3

430
G2525
Oryza sativa ( indica
gi5679839

0.46



cultivar-group)

430
G2525

Chlamydomonas

gi3342148

0.58




reinhardtii


430
G2525

Brassica juncea

gi32527767

0.94

430
G2525

Zea mays

gi100920

0.99

430
G2525

Helianthus praecox

gi18073228

1

430
G2525

Helianthus niveus

gi27526450

1

431
G2536

Glycine max

GLYMA-28NOV01-
1163




CLUSTER325924_1

431
G2536

Glycine max

GLYMA-28NOV01-
1164




CLUSTER325924_2

431
G2536

Glycine max

GLYMA-28NOV01-
1165




CLUSTER325924_3

431
G2536

Glycine max

GLYMA-28NOV01-
1166




CLUSTER90132_1

431
G2536

Glycine max

GLYMA-28NOV01-
1167




CLUSTER90912_1

431
G2536

Oryza sativa

BE039729.1
1168

431
G2536

Oryza sativa

BE039742.1
1169

431
G2536

Oryza sativa

OSC12121.C1.p10.fg
1170

431
G2536

Oryza sativa

OSC8882.C1.p8.fg
1171

431
G2536

Zea mays

ZEAMA-08NOV01-
1172




CLUSTER460898_1

431
G2536

Lycopersicon

SGN-UNIGENE-56029
2060




esculentum


431
G2536

Lycopersicon

SGN-UNIGENE-
2061




esculentum

SINGLET-36423

432
G2536

Populus tremula x

BU815065

5.00E−57




Populus tremuloides


432
G2536

Nicotiana tabacum

AB021178

1.00E−53

432
G2536

Medicago

B0647353

2.00E−52




truncatula


432
G2536

Populus

CA825708

1.00E−48



balsamifera subsp.




trichocarpa x





Populus deltoides


432
G2536

Lycopersicon

AW030267

3.00E−47




esculentum


432
G2536

Brassica oleracea

BZ032987

1.00E−45

432
G2536

Glycine max

BM886486

1.00E−45

432
G2536

Lactuca sativa

BQ875839

4.00E−44

432
G2536

Oryza sativa

AK063703

2.00E−43



( japonica cultivar-



group)

432
G2536

Zea mays

CC673415

2.00E−42

432
G2536

Nicotiana tabacum

gi25457840

1.00E−50

432
G2536

Oryza sativa

gi15290110

6.50E−45

432
G2536

Brassica napus

gi31322582

5.10E−30

432
G2536

Oryza sativa

gi24899399

8.10E−28



( japonica cultivar-



group)

432
G2536

Petunia x hybrida

gi21105734

3.40E−27

432
G2536

Solanum tuberosum

gi14485513

1.20E−26

432
G2536

Glycine max

gi22597158

1.50E−26

432
G2536

Lycopersicon

gi6175246

1.50E−26




esculentum


432
G2536

Phaseolus vulgaris

gi15148914

6.40E−26

432
G2536
Triticum sp.
gi4218535

8.50E−24

434
G2543
Phalaenopsis sp.
PSU34743

1.0e−999



SM9108

434
G2543

Picea abies

AF172931

1.0e−999

434
G2543

Oryza sativa

AB101645

1.00E−173



( japonica cultivar-



group)

434
G2543

Gossypium

AF530914

1.00E−173




hirsutum


434
G2543

Oryza sativa

AB077993

1.00E−172

434
G2543
Oryza sativa ( indica
AAAA01007245

1.00E−170



cultivar-group)

434
G2543

Zea mays

ZMA250987

1.00E−170

434
G2543

Malus domestica

AF067961

1.00E−146

434
G2543

Helianthus annuus

HNNHAHR

1.00E−118

434
G2543

Vitis vinifera

CB002690

2.00E−93

434
G2543
Phalaenopsis sp.
gi1173622

5.10E−175



SM9108

434
G2543
Phalaenopsis sp.
gi2147484

5.10E−175

434
G2543

Picea abies

gi12002853

4.80E−172

434
G2543

Oryza sativa

gi32488795

4.70E−165



( japonica cultivar-



group)

434
G2543

Gossypium

gi22475197

6.00E−165




hirsutum


434
G2543

Sorghum bicolor

gi18481701

7.70E−165

434
G2543

Oryza sativa

gi19072102

5.40E−164

434
G2543

Zea mays

gi8920427

4.40E−162

434
G2543

Malus x domestica

gi3925363

2.10E−139

434
G2543

Helianthus annuus

gi1208940

1.90E−106

435
G2550

Glycine max

GLYMA-28NOV01-
1173




CLUSTER39853_1

435
G2550

Glycine max

GLYMA-28NOV01-
1174




CLUSTER39853_2

435
G2550

Oryza sativa

uC-osroM202005g06b1
1175

435
G2550

Oryza sativa

Os_S107153
1610

435
G2550

Medicago

Mtr_S5360919
1711




truncatula


435
G2550

Medicago

Mtr_S7094331
1712




truncatula


435
G2550

Zea mays

Zm_S11465618
1815

435
G2550

Triticum aestivum

Ta_S146249
1905

436
G2550

Solanum tuberosum

AF406703

9.00E−90

436
G2550

Oryza sativa

AK069994

6.00E−89



( japonica cultivar-



group)

436
G2550

Brassica oleracea

BZ485520

1.00E−82

436
G2550

Lycopersicon

AF375966

2.00E−77




esculentum


436
G2550

Sorghum bicolor

BM324585

5.00E−74

436
G2550

Malus x domestica

AF053769

2.00E−70

436
G2550

Oryzaminuta

CB213421

3.00E−70

436
G2550

Hordeum vulgare

BG344928

5.00E−70

436
G2550

Medicago

AW688195

8.00E−67




truncatula


436
G2550
Oryza sativa ( indica
CB618008

3.00E−66



cultivar-group)

436
G2550

Solanum tuberosum

gi22652127

1.40E−87

436
G2550

Oryza sativa

gi12656811

1.60E−84

436
G2550

Oryza sativa

gi20219036

1.20E−83



( japonica cultivar-



group)

436
G2550

Lycopersicon

gi31323447

4.40E−75




esculentum


436
G2550

Hordeum vulgare

gi13752407

1.70E−72

436
G2550

Malus x domestica

gi7239157

9.40E−72

436
G2550

Gnetum gnemon

gi31746344

5.50E−66

436
G2550
Oryza sativa ( indica
gi19352101

4.00E−49



cultivar-group)

436
G2550

Zea mays

gi19743685

1.90E−42

436
G2550

Helianthus annuus

gi20977644

6.30E−12

438
G2559

Solanum tuberosum

STU72489

1.00E−59

438
G2559

Lycopersicon

AF123265

2.00E−59




esculentum


438
G2559

Antirrhinum majus

AJ559687

3.00E−54

438
G2559

Vitis vinifera

CB344101

2.00E−53

438
G2559

Glycine max

CA799179

3.00E−53

438
G2559

Ipomoea nil

BJ573275

9.00E−53

438
G2559

Citrus sinensis

CB292623

2.00E−52

438
G2559

Gossypium

BQ411282

6.00E−52




arboreum


438
G2559

Oryza sativa

AK105097

8.00E−52



( japonica cultivar-



group)

438
G2559

Medicago

BM779097

1.00E−51




truncatula


438
G2559

Solanum tuberosum

gi1881585

7.10E−59

438
G2559

Lycopersicon

gi4731573

3.90E−58




esculentum


438
G2559

Oryza sativa

gi32489830

1.50E−51



( japonica cultivar-



group)

438
G2559

Lotus japonicus

gi2367429

9.80E−05

438
G2559

Zea mays

gi23928441

0.00023

438
G2559

Plastid Oenothera

gi13276714

0.00077



elata subsp. hookeri

438
G2559

Oenothera elata

gi23822375

0.00077



subsp. hookeri

438
G2559

Nicotiana tabacum

gi8096269

0.0013

438
G2559

Cicer arietinum

gi3129939

0.0014

438
G2559

Solanum berthaultii

gi1216214

0.002

440
G2565

Mesembryanthemum

AF219972

5.00E−67




crystallinum


440
G2565

Nicotiana tabacum

AB017693

1.00E−60

440
G2565

Triticum aestivum

BQ806133

3.00E−55

440
G2565

Oryza sativa

AK109510

6.00E−55



( japonica cultivar-



group)

440
G2565

Beta vulgaris

BQ587750

1.00E−52

440
G2565

Medicago

AW684291

1.00E−51




truncatula


440
G2565

Zea mays

AY107734

2.00E−51

440
G2565

Glycine max

AW507631

2.00E−51

440
G2565

Lycopersicon

AW030183

8.00E−51




esculentum


440
G2565

Hordeum vulgare

BU993345

8.00E−46

440
G2565

Mesembryanthemum

gi6942190

3.50E−66




crystallinum


440
G2565

Nicotiana tabacum

gi4519671

2.80E−57

440
G2565

Oryza sativa

gi29647445

3.90E−42



( japonica cultivar-



group)

440
G2565

Solanum

gi32470629

6.00E−30




bulbocastanum


440
G2565

Chlamydomonas

gi5916207

2.30E−24




reinhardtii


440
G2565

Oryza sativa

gi11034542

1.30E−09

440
G2565

Zea mays

gi15667625

2.60E−08

440
G2565

Oryza glaberrima

gi31338862

2.70E−07

440
G2565
Oryza sativa ( indica
gi31338860

7.40E−07



cultivar-group)

440
G2565

Pachysandra

gi32478053

0.0038



terminalis

441
G2567

Glycine max

GLYMA-28NOV01-
1176




CLUSTER105903_1

441
G2567

Glycine max

GLYMA-28NOV01-
1177




CLUSTER25797_1

441
G2567

Glycine max

GLYMA-28NOV01-
1178




CLUSTER25797_2

441
G2567

Glycine max

GLYMA-28NOV01-
1179




CLUSTER25797_4

441
G2567

Glycine max

GLYMA-28NOV01-
1180




CLUSTER30315_1

441
G2567

Glycine max

GLYMA-28NOV01-
1181




CLUSTER67014_1

441
G2567

Oryza sativa

ORYSA-22JAN02-
1182




CLUSTER14787_2

441
G2567

Oryza sativa

ORYSA-22JAN02-
1183




CLUSTER14787_3

441
G2567

Oryza sativa

OSC100544.C1.p6.fg
1184

441
G2567

Oryza sativa

OSC100702.C1.pl1.fg
1185

441
G2567

Oryza sativa

OSC19346.C1.p18.fg
1186

441
G2567

Zea mays

ZEAMA-08NOV01-
1187




CLUSTER28837_1

441
G2567

Zea mays

ZEAMA-08NOV01-
1188




CLUSTER536_1816

441
G2567

Zea mays

ZEAMA-08NOV01-
1189




CLUSTER536_BYHAN




D_1816

441
G2567

Zea mays

ZEAMA-08NOV01-
1190




CLUSTER64018_1

441
G2567

Zea mays

ZEAMA-08NOV01-
1191




CLUSTER64018_4

441
G2567

Oryza sativa

Os_S45221
1611

441
G2567

Oryza sativa

Os_S99383
1612

441
G2567

Glycine max

Gma_S5079377
1669

441
G2567

Hordeum vulgare

Hv_S31206
1743

441
G2567

Zea mays

Zm_S11521593
1816

441
G2567

Triticum aestivum

Ta_S114520
1906

441
G2567

Lycopersicon

SGN-UNIGENE-
2062




esculentum

SINGLET-357264

441
G2567

Lycopersicon

SGN-UNIGENE-
2063




esculentum

SINGLET-360651

441
G2567

Lycopersicon

SGN-UNIGENE-
2064




esculentum

SINGLET-463424

442
G2567

Oryza sativa

AK100322

1.0e−999



( japonica cultivar-



group)

442
G2567

Oryza sativa

ABO71299

1.0e−999

442
G2567
Oryza sativa ( indica
AAAA01008877

1.00E−172



cultivar-group)

442
G2567

Lotus japonicus

AP004505

1.00E−140

442
G2567

Physcomitrella

AX288142

1.00E−131




patens


442
G2567

Brassica oleracea

BZ016919

1.00E−124

442
G2567

Medicago

BG646821

1.00E−104




truncatula


442
G2567

Brassica napus

CD843527

1.00E−100

442
G2567

Zea mays

GC657791

2.00E−99

442
G2567

Vitis vinifera

CB979491

2.00E−90

442
G2567

Oryza sativa

gi13384374

3.80E−186



( japonica cultivar-



group)

442
G2567

Oryza sativa

gi19352051

3.30E−164

442
G2567

Prunus persica

gi27450533

8.50E−72

442
G2567
Oryza sativa ( indica
gi26251300

4.30E−70



cultivar-group)

442
G2567

Mangifera indica

gi30027167

2.00E−68

442
G2567

Marchantia

gi25272004

5.40E−13




polymorpha


442
G2567

Mirabilis jalapa

gi23343944

3.20E−05

442
G2567

Zea mays

gi18697008

0.014

442
G2567

Cucumis sativus

gi6136830

0.049

442
G2567

Nicotiana tabacum

gi4887014

0.087

444
G2570

Brassica oleracea

BH973299

3.00E−49

444
G2570

Zea mays

ABO60130

3.00E−32

444
G2570

Oryza sativa

AK101165

1.00E−28



( japonica cultivar-



group)

444
G2570
Oryza sativa ( indica
CB630542

1.00E−25



cultivar-group)

444
G2570

Medicago

CB891281

3.00E−22




truncatula


444
G2570

Brassica napus

CD825309

2.00E−20

444
G2570

Solanum tuberosum

BM407041

3.00E−20

444
G2570

Triticum aestivum

BQ743733

2.00E−17

444
G2570

Hordeum vulgare

CA014844

5.00E−17



subsp. vulgare

444
G2570

Stevia rebaudiana

BG525366

9.00E−17

444
G2570

Zea mays

gi13661174

3.10E−31

444
G2570

Oryza sativa

gi24308616

4.60E−31



( japonica cultivar-



group)

444
G2570

Oryza glaberrima

gi31338862

1.90E−23

444
G2570
Oryza sativa ( indica
gi31338860

2.50E−23



cultivar-group)

444
G2570

Oryza sativa

gi15289981

4.10E−16

444
G2570

Dianthus

gi13173408

1.50E−08




caryophyllus


444
G2570

Chlamydomonas

gi5916207

2.40E−07




reinhardtii


444
G2570

Solanum

gi32470629

8.30E−06




bulbocastanum


444
G2570

Nicotiana tabacum

gi4519671

1.50E−05

444
G2570

Mesembryanthemum

gi6942190

0.00022




crystallinum


445
G2571

Glycine max

GLYMA-28NOV01-
1192




CLUSTER96988_1

445
G2571

Glycine max

GLYMA-28NOV01-
1193




CLUSTER96988_2

445
G2571

Oryza sativa

OSC1398.C1.p2.fg
1194

445
G2571

Lycopersicon

SGN-UNIGENE-56732
2065




esculentum


446
G2571

Brassica oleracea

BZ432711

1.00E−103

446
G2571

Glycine max

AW759250

2.00E−48

446
G2571
Oryza sativa ( indica
AAAA01008818

6.00E−48



cultivar-group)

446
G2571

Oryza sativa

AC104284

8.00E−48

446
G2571

Medicago

BQ140137

1.00E−44




truncatula


446
G2571

Zea mays

CC342129

8.00E−41

446
G2571

Lactuca sativa

BQ849477

2.00E−38

446
G2571

Sorghum bicolor

CD236030

3.00E−38

446
G2571

A triplex hortensis

AF274033

3.00E−34

446
G2571

Prunus persica

BU046010

2.00E−33

446
G2571

Oryza sativa

gi19920190

2.60E−36



( japonica cultivar-



group)

446
G2571

Atriplex hortensis

gi8571476

1.90E−35

446
G2571

Lycopersicon

gi27436378

1.30E−34




esculentum


446
G2571

Zea mays

gi21908036

9.10E−27

446
G2571

Oryza sativa

gi5091503

3.90E−26

446
G2571

Solanum tuberosum

gi1688233

1.30E−20

446
G2571

Nicotiana sylvestris

gi8809571

5.50E−20

446
G2571

Nicotiana tabacum

gi1208498

1.50E−19

446
G2571

Glycine max

gi21304712

1.10E−18

446
G2571

Prunus armeniaca

gi3264767

7.70E−18

448
G2574

Brassica oleracea

BZ016708

1.00E−82

448
G2574

Oryza sativa

AX653450

5.00E−80

448
G2574

Nicotiana tabacum

AY220477

3.00E−68

448
G2574
Oryza sativa ( indica
AAAA01001635

2.00E−64



cultivar-group)

448
G2574

Lycopersicon

BI922133

6.00E−56




esculentum


448
G2574

Hordeum vulgare

BM370908

2.00E−47

448
G2574

Medicago

GB893379

4.00E−47




truncatula


448
G2574

Glycine max

BU926713

4.00E−44

448
G2574

Citrus sinensis

BQ625082

4.00E−44

448
G2574

Zea mays

AX660892

6.00E−44

448
G2574

Oryza sativa

gi11320830

4.10E−61

448
G2574

Nicotiana tabacum

gi30013667

5.60E−59

448
G2574

Oryza sativa

gi20160973

4.10E−37



( japonica cultivar-



group)

448
G2574

Petroselinum

gi11493822

6.80E−30




crispum


448
G2574

Avena fatua

gi1159879

5.10E−26

448
G2574

Avena sativa

gi4894965

2.10E−22

448
G2574

Glycine max

gi32493108

2.20E−20

448
G2574

Ipomoea batatas

gi1076685

5.20E−20

448
G2574

Capsella rubella

gi32454266

8.00E−20

448
G2574

Lycopersicon

gi13620227

2.20E−18




esculentum


450
G2575

Lycopersicon

BI205259

1.00E−48




esculentum


450
G2575

Medicago

BI308031

5.00E−45




truncatula


450
G2575

Vitis vinifera

CD010167

1.00E−44

450
G2575

Sorghum bicolor

BE362650

2.00E−43

450
G2575

Oryza sativa

AX653470

2.00E−43

450
G2575

Triticum aestivum

CD883886

7.00E−42

450
G2575

Amborella

CD483414

1.00E−41




trichopoda


450
G2575

Oryza sativa

AK108745

3.00E−41



( japonica cultivar-



group)

450
G2575

Glycine max

GA784851

3.00E−41

450
G2575

Populus tremula x

BU884581

3.00E−38




Populus tremuloides


450
G2575

Oryza sativa

gi11761085

6.50E−45

450
G2575

Oryza sativa

gi22830985

4.30E−36



( japonica cultivar-



group)

450
G2575

Nicotiana tabacum

gi25348323

2.00E−30

450
G2575

Solanum tuberosum

gi24745606

3.00E−28

450
G2575
Oryza sativa ( indica
gi23305051

6.80E−28



cultivar-group)

450
G2575

Lycopersicon

gi13620227

9.90E−27




esculentum


450
G2575

Cucumis sativus

gi7484759

1.70E−26

450
G2575

Pimpinella

gi3420906

1.80E−26




brachycarpa


450
G2575

Avena fatua

gi1159877

1.90E−26

450
G2575

Avena sativa

gi4894965

2.10E−26

451
G2579

Oryza sativa

OSC101983.C1.p6.fg
1195

451
G2579

Zea mays

LIB3076-043-Q1-K1-H9
1196

451
G2579

Zea mays

Zm_S11519895
1817

452
G2579

Brassica oleracea

BZ471318

2.00E−82

452
G2579

Oryza sativa

AP004016

3.00E−34

452
G2579

Oryza sativa

AP004570

3.00E−34



( japonica cultivar-



group)

452
G2579
Oryza sativa ( indica
AAAA01001296

3.00E−34



cultivar-group)

452
G2579

Zea mays

AY178191

1.00E−32

452
G2579

Sorghum bicolor

BZ338708

1.00E−23

452
G2579

Medicago

AC119414

1.00E−21




truncatula


452
G2579

Beta vulgaris

BQ488935

1.00E−19

452
G2579

Lotus japonicus

AV411846

2.00E−19

452
G2579

Glycine max

BE059333

7.00E−19

452
G2579

Zea mays

gi27802487

5.50E−38

452
G2579

Oryza sativa

gi23307521

1.50E−35



( japonica cultivar-



group)

452
G2579

Stylosanthes hamata

gi4099921

1.30E−19

452
G2579

Cicer arietinum

gi24817250

5.50E−19

452
G2579

Nicotiana tabacum

gi4587373

8.70E−19

452
G2579

Oryza sativa

gi12597874

1.90E−18

452
G2579

Lycopersicon

gi2213785

5.90E−18




esculentum


452
G2579

Prunus armeniaca

gi3264767

5.90E−18

452
G2579

Thellungiella

gi20340233

9.90E−18




halophila


452
G2579

Nicotiana sylvestris

gi8809573

1.30E−17

453
G2585

Oryza sativa

OSC102182.C1.p9.fg
1197

453
G2585

Oryza sativa

OSC5912.C1.p4.fg
1198

453
G2585

Zea mays

ZEAMA-08NOV01-
1199




CLUSTER221367_1

454
G2585

Brassica oleracea

BH574869

1.00E−71

454
G2585

Populus tremula x

BU814191

9.00E−23




Populus tremuloides


454
G2585

Glycine max

BQ612521

8.00E−22

454
G2585

Lycopersicon

BG127023

4.00E−21




esculentum


454
G2585

Solanum tuberosum

BQ118881

1.00E−19

454
G2585

Medicago

AW776895

2.00E−19




truncatula


454
G2585

Gossypium

CA992948

2.00E−19




hirsutum


454
G2585

Nicotiana tabacum

AF193771

3.00E−19

454
G2585

Oryza sativa

AC144737

5.00E−18



( japonica cultivar-



group)

454
G2585
Oryza sativa ( indica
AAAA01016983

5.00E−18



cultivar-group)

454
G2585

Nicotiana tabacum

gi11358951

3.30E−22

454
G2585

Oryza sativa

gi18844822

5.30E−17



( japonica cultivar-



group)

454
G2585

Matricaria

gi17385638

6.50E−17




chamomilla


454
G2585

Solanum tuberosum

gi24745606

1.90E−12

454
G2585

Oryza sativa

gi14209559

1.20E−11

454
G2585

Petroselinum

gi11493824

1.70E−11




crispum


454
G2585

Retama raetam

gi18158619

1.10E−09

454
G2585
Oryza sativa ( indica
gi23305051

3.30E−09



cultivar-group)

454
G2585

Solanum dulcamara

gi16588566

8.20E−09

454
G2585

Capsella rubella

gi27817201

1.10E−08

456
G2587

Lycopersicon

BG642706

5.00E−23




esculentum


456
G2587

Solanum tuberosum

BE472874

1.00E−22

456
G2587

Medicago

AW560120

6.00E−22




truncatula


456
G2587

Populus tremula x

BU816132

1.00E−20




Populus tremuloides


456
G2587

Glycine max

BQ612521

3.00E−20

456
G2587

Nicotiana tabacum

AF193770

6.00E−19

456
G2587

Gossypium

GA992948

7.00E−18




hirsutum


456
G2587

Triticum aestivum

BG605176

2.00E−16

456
G2587

Vitis vinifera

CA813725

6.00E−16

456
G2587

Lactuca sativa

BQ875950

8.00E−16

456
G2587

Nicotiana tabacum

gi7406995

5.40E−22

456
G2587

Matricaria

gi17385638

1.10E−16




chamomilla


456
G2587

Oryza sativa

gi18844816

5.90E−16



( japonica cultivar-



group)

456
G2587

Petroselinum

gi11493824

5.20E−13




crispum


456
G2587

Oryza sativa

gi15289994

1.30E−11

456
G2587

Solanum tuberosum

gi24745606

3.00E−11

456
G2587

Capsella rubella

gi27817201

2.90E−09

456
G2587

Solanum dulcamara

gi16588566

5.10E−09

456
G2587

Avena fatua

gi1159879

1.80E−08

456
G2587

Dactylis glomerata

gi11993901

2.20E−08

458
G2592

Oryza sativa

AK103583

1.00E−154



( japonica cultivar-



group)

458
G2592

Lemna paucicostata

AB023895

1.00E−131

458
G2592

Zea mays

AY109994

1.00E−128

458
G2592

Cicer arietinum

CAR400860

1.00E−105

458
G2592

Solanum tuberosum

BG351697

1.00E−104

458
G2592

Lotus japonicus

AP004525

1.00E−103

458
G2592

Medicago

CB894562

1.00E−100




truncatula


458
G2592

Helianthus annuus

BQ970590

3.00E−96

458
G2592

Gossypium

BQ406633

3.00E−93




arboreum


458
G2592

Oryza sativa

AC105318

7.00E−93

458
G2592

Oryza sativa

gi32480039

1.10E−146



( japonica cultivar-



group)

458
G2592

Oryza sativa

gi5777631

1.10E−146

458
G2592

Lemna paucicostata

gi5689214

2.90E−124

458
G2592

Cicer arietinum

gi7635492

7.70E−101

458
G2592

Pyrus communis

gi18252343

2.90E−56

458
G2592

Chloroplast Pinus

gi1262738

1




thunbergii


460
G2597

Brassica oleracea

BH974513

6.00E−35

460
G2597

Vitis vinifera

CB977099

6.00E−35

460
G2597

Glycine max

AW760239

2.00E−33

460
G2597

Helianthus annuus

BQ966485

9.00E−33

460
G2597

Lactuca sativa

BQ853988

1.00E−32

460
G2597

Lotus corniculatus

CB827195

2.00E−32



var. japonicus

460
G2597

Medicago

BI309254

2.00E−31




truncatula


460
G2597

Lycopersicon

AI895129

4.00E−30




esculentum


460
G2597

Solanum tuberosum

BE919865

7.00E−30

460
G2597

Lupinus albus

CA410490

8.00E−29

460
G2597

Oryza sativa

gi23495868

4.20E−39



( japonica cultivar-



group)

460
G2597

Lemna paucicostata

gi5689214

5.90E−35

460
G2597

Oryza sativa

gi5777631

3.60E−33

460
G2597

Cicer arietinum

gi7635492

4.70E−33

460
G2597

Pyrus communis

gi18252343

0.018

462
G2603

Oryza sativa

AK060587

1.00E−116



( japonica cultivar-



group)

462
G2603

Brassica oleracea

BZ462499

3.00E−95

462
G2603

Zea mays

AY106234

9.00E−95

462
G2603

Lemna paucicostata

AB023895

2.00E−92

462
G2603

Cicer arietinum

CAR400860

3.00E−92

462
G2603

Descurainia sophia

BU238485

1.00E−84

462
G2603

Brassica napus

CD819805

2.00E−83

462
G2603

Medicago

BF004440

2.00E−76




truncatula


462
G2603

Vitis vinifera

CB977099

3.00E−75

462
G2603

Triticum aestivum

CA485505

6.00E−73

462
G2603

Oryza sativa

gi23495868

9.40E−112



( japonica cultivar-



group)

462
G2603

Lemna paucicostata

gi5689214

4.80E−91

462
G2603

Cicer arietinum

gi7635492

1.90E−88

462
G2603

Oryza sativa

gi5777631

2.70E−84

462
G2603

Pyrus communis

gi18252343

6.90E−61

464
G2604

Medicago

BF519786

2.00E−74




truncatula


464
G2604

Solanum tuberosum

BQ118459

8.00E−74

464
G2604

Lycopersicon

BF051817

5.00E−73




esculentum


464
G2604

Populus tremula x

BU863675

5.00E−73




Populus tremuloides


464
G2604

Populus tremula

BU892953

5.00E−73

464
G2604

Glycine max

CD487599

3.00E−72

464
G2604

Helianthus

CF079899

2.00E−70




paradoxus


464
G2604

Oryza sativa

CB680709

9.00E−67



( japonica cultivar-



group)

464
G2604

Sorghum

BG488424

6.00E−66




ropinquum


464
G2604

Triticum aestivum

BJ302536

6.00E−66

464
G2604

Oryza sativa

gi29467554

6.50E−65



( japonica cultivar-



group)

464
G2604

Zea mays

gi13509835

3.40E−43

464
G2604

Brassica oleracea

gi17981380

2.40E−35

464
G2604

Triticum aestivum

gi32400832

2.20E−25

464
G2604

Oryza sativa

gi11034561

1.80E−21

464
G2604

Hordeum vulgare

gi23954355

0.067



subsp. vulgare

464
G2604

Phaseolus vulgaris

gi6863082

0.087

464
G2604

Vigna angularis

gi224356

0.18

464
G2604

Gossypium

gi28274033

0.18




barbadense


464
G2604

Lycopersicon

gi575950

0.18




esculentum


466
G2616

Brassica oleracea

BH511341

2.00E−47

466
G2616

Populus tremula x

BD194427

6.00E−37




Populus tremuloides


466
G2616

Lycopersicon

BI930760

1.00E−36




esculentum


466
G2616

Solanum tuberosum

BE472904

4.00E−36

466
G2616

Oryza sativa

AK102788

2.00E−35



( japonica cultivar-



group)

466
G2616

Helianthus annuus

AJ412302

2.00E−35

466
G2616

Prunus persica

BU045784

9.00E−35

466
G2616

Hordeum vulgare

CA032614

2.00E−34



subsp. vulgare

466
G2616

Glycine max

BM520826

4.00E−34

466
G2616

Medicago

BE202697

1.00E−33




truncatula


466
G2616

Populus tremula x

gi3955021

2.60E−38




Populus tremuloides


466
G2616

Oryza sativa

gi15128400

1.30E−36

466
G2616

Oryza sativa

gi21104626

4.80E−19



( japonica cultivar-



group)

466
G2616

Petunia x hybrida

gi22087128

1.80E−11

466
G2616

Lycopersicon

gi28070968

3.20E−11




esculentum


466
G2616

Narcissus

gi18419580

7.20E−11




pseudonarcissus


466
G2616

Ceratopteris

gi3868847

0.00018



richardii

466
G2616

Craterostigma

gi3171739

0.002




plantagineum


466
G2616

Capsella rubella

gi8919876

0.0025

466
G2616

Physcomitrella

gi7415620

0.0068




patens


467
G2617

Oryza sativa

jC-
1200




osroLIB3475060d09b1

468
G2617

Brassica oleracea

BH732988

3.00E−66

468
G2617

Brassica rapa

BZ614339

2.00E−54



subsp. pekinensis

468
G2617

Medicago

AC135233

2.00E−19




truncatula


468
G2617

Oryza sativa

AC133008

4.00E−19

468
G2617

Glycine max

BG363109

4.00E−19

468
G2617

Oryza sativa

AK108829

7.00E−19



( japonica cultivar-



group)

468
G2617
Oryza sativa ( indica
AAAA01006129

7.00E−19



cultivar-group)

468
G2617

Zea mays

BZ735433

2.00E−18

468
G2617

Hordeum vulgare

BF255914

9.00E−16

468
G2617

Helianthus annuus

BQ977193

1.00E−13

468
G2617

Oryza sativa

gi32489630

2.60E−18



( japonica cultivar-



group)

468
G2617

Zea ramosa

gi18674684

6.20E−14

468
G2617

Petunia x hybrida

gi14275902

9.10E−12

468
G2617

Oryza sativa

gi15528588

1.80E−05

468
G2617

Sorghum bicolor

gi18390109

0.00014

468
G2617

Datisca glomerata

gi4666360

0.006

468
G2617

Pisum sativum

gi2129892

0.0097

468
G2617

Triticum aestivum

gi485814

0.02

468
G2617

Brassica rapa

gi2058504

0.021

468
G2617

Glycine max

gi1763063

0.029

470
G2628

Brassica oleracea

BH962006

1.00E−21

470
G2628

Mesembryanthemum

BF479304

4.00E−08




crystallinum


470
G2628

Lycopersicon

BE450859

9.00E−08




esculentum


470
G2628

Nicotiana tabacum

BP130848

3.00E−07

470
G2628

Glycine max

BF595900

3.00E−07

470
G2628

Phaseolus vulgaris

AF350505

8.00E−07

470
G2628

Lotus japonicus

BI417596

1.00E−06

470
G2628

Rosa chinensis

BI977302

1.00E−06

470
G2628
Oryza sativa ( indica
AAAA01003396

1.00E−06



cultivar-group)

470
G2628

Medicago

BE943475

2.00E−06




truncatula


470
G2628

Phaseolus vulgaris

gi13430400

6.40E−10

470
G2628

Phaseolus

gi12829956

1.70E−09



acutifolius

470
G2628

Raphanus sativus

gi1033059

2.70E−08

470
G2628

Sinapis alba

gi2995462

5.00E−08

470
G2628

Petroselinum

gi9650826

1.80E−07




crispum


470
G2628

Brassica napus

gi633154

2.40E−07

470
G2628

Glycine max

gi169961

3.20E−07

470
G2628

Oryza sativa

gi13365774

4.40E−07

470
G2628

Nicotiana tabacum

gi10241920

6.00E−07

470
G2628

Oryza sativa

gi18844791

1.80E−06



( japonica cultivar-



group)

472
G2632

Brassica napus

BNU33885

1.00E−121

472
G2632

Brassica oleracea

BZ466456

2.00E−61

472
G2632

Medicago

BI263541

2.00E−53




truncatula


472
G2632

Citrus sinensis

BQ624240

1.00E−48

472
G2632

Prunus dulcis

BU573158

1.00E−48

472
G2632

Helianthus

CF082573

1.00E−38




paradoxus


472
G2632

Amborella

CD484119

5.00E−36




trichopoda


472
G2632

Oryza sativa

AK069854

8.00E−36



( japonica cultivar-



group)

472
G2632

Lycopersicon

BF113081

1.00E−35




esculentum


472
G2632

Triticum aestivum

BT009512

5.00E−34

472
G2632

Brassica napus

gi1173618

1.10E−115

472
G2632

Oryza sativa

gi27552556

5.30E−38



( japonica cultivar-



group)

472
G2632

Vitis riparia

gi7141243

1.50E−30

472
G2632

Oryza sativa

gi2826786

2.00E−29

472
G2632

Nicotiana tabacum

gi4731314

7.00E−20

472
G2632

Triticum aestivum

gi1076781

0.05

472
G2632

Antirrhinum majus

gi28894445

0.056

472
G2632

Solanum tuberosum

gi7688355

0.27

472
G2632

Vigna unguiculata

gi9857292

0.38

472
G2632

Zea mays

gi829240

0.79

474
G2633

Medicago

AC121232

1.00E−160




truncatula


474
G2633

Oryza sativa

AE017084

1.00E−159



( japonica cultivar-



group)

474
G2633
Oryza sativa ( indica
AAAA01002412

1.00E−159



cultivar-group)

474
G2633

Oryza sativa

AP003747

1.00E−154

474
G2633

Zea mays

AY109543

1.00E−145

474
G2633

Vitis vinifera

CB980495

1.00E−124

474
G2633

Solanum tuberosum

BG595264

1.00E−103

474
G2633

Brassica oleracea

BH591768

1.00E−103

474
G2633

Glycine max

CA800623

1.00E−102

474
G2633

Lycopersicon

BI422927

4.00E−94




esculentum


474
G2633

Oryza sativa

gi20043021

8.30E−152



( japonica cultivar-



group)

474
G2633

Brassica rapa

gi28143934

9.60E−71



subsp. pekinensis

474
G2633

Vitis vinifera

gi20334379

6.90E−61

474
G2633

Lycopersicon

gi31322802

1.10E−60




esculentum


474
G2633

Lilium longiflorum

gi32813435

3.80E−60

474
G2633

Oryza sativa

gi14719333

2.20E−57

474
G2633

Gossypium

gi29122893

7.30E−57




hirsutum


474
G2633

Argyroxiphium

gi20257438

9.40E−57



sandwicense subsp.




macrocephalum


474
G2633

Wilkesia

gi20257432

1.50E−56




gymnoxiphium


474
G2633

Calycadenia

gi20257451

1.50E−56




multiglandulosa


476
G2636

Petunia x hybrida

PHRNANAM

5.00E−80

476
G2636

Lactuca sativa

BQ994853

1.00E−71

476
G2636

Medicago

BI308121

2.00E−70




truncatula


476
G2636

Glycine max

AF532619

1.00E−68

476
G2636

Oryza sativa

AP004679

7.00E−65



( japonica cultivar-



group)

476
G2636

Oryza sativa

AP003542

7.00E−65

476
G2636

Brassica napus

CD830372

6.00E−62

476
G2636

Thellungiella

BM985861

1.00E−61




halophila


476
G2636

Lycopersicon

AI898478

1.00E−61




esculentum


476
G2636

Solanum tuberosum

BQ518471

1.00E−61

476
G2636

Petunia x hybrida

gi1279640

1.60E−75

476
G2636

Glycine max

gi22597158

3.00E−67

476
G2636

Oryza sativa

gi27529810

1.30E−59

476
G2636

Zea mays

gi32527660

5.50E−58

476
G2636

Oryza sativa

gi28411877

7.50E−58



( japonica cultivar-



group)

476
G2636
Triticum sp.
gi4218537

4.20E−54

476
G2636

Triticum

gi6732160

4.20E−54




monococcum


476
G2636

Phaseolus vulgaris

gi15148912

1.70E−50

476
G2636

Lycopersicon

gi6175246

3.00E−44




esculentum


476
G2636

Brassica napus

gi31322568

7.90E−44

478
G2639

Brassica oleracea

BH678678

1.00E−67

478
G2639

Medicago

BE998137

7.00E−47




truncatula


478
G2639

Populus tremula x

BU826518

2.00E−43




Populus tremuloides


478
G2639

Oryza sativa

AK102444

3.00E−41



( japonica cultivar-



group)

478
G2639

Lycopersicon

BI208719

2.00E−38




esculentum


478
G2639

Oryza sativa

AP003683

5.00E−38

478
G2639

Oryza sativa (
indica

AAAA01008013

5.00E−38



cultivar-group)

478
G2639

Hevea brasiliensis

CB376600

1.00E−37

478
G2639

Zea mays

CG628094

4.00E−36

478
G2639

Hordeum vulgare

CA029965

1.00E−33



subsp. vulgare

478
G2639

Oryza sativa

gi15528818

2.20E−41

478
G2639

Oryza sativa

gi20161862

2.20E−41



( japonica cultivar-



group)

478
G2639

Pinus pinaster

gi18129286

0.68

478
G2639

Zea mays

gi7489714

0.74

478
G2639

Triticum aestivum

gi170732

1

478
G2639

Populus tremuloides

gi9651406

1

480
G2640

Brassica oleracea

BH678678

1.00E−107

480
G2640

Populus tremula x

BU826518

1.00E−54




Populus tremuloides


480
G2640

Medicago

BE998137

1.00E−52




truncatula


480
G2640

Oryza sativa

AK102444

1.00E−42



( japonica cultivar-



group)

480
G2640

Hevea brasiliensis

CB376600

2.00E−40

480
G2640

Lycopersicon

BI205918

3.00E−40




esculentum


480
G2640

Oryza sativa

AP003683

4.00E−40

480
G2640
Oryza sativa ( indica
AAAA01008013

4.00E−40



cultivar-group)

480
G2640

Zea mays

CC628094

2.00E−37

480
G2640

Hordeum vulgare

CA029965

7.00E−35



subsp. vulgare

480
G2640

Oryza sativa

gi15528818

1.90E−45

480
G2640

Oryza sativa

gi20161862

1.90E−45



( japonica cultivar-



group)

480
G2640

Zea mays

gi7248461

0.00063

480
G2640

Brassica napus

gi17821

0.0011

480
G2640

Triticum aestivum

gi21322752

0.0012

480
G2640

Chlamydomonas

gi32454910

0.0022




reinhardtii


480
G2640

Phaseolus vulgaris

gi121628

0.0042

480
G2640

Lycopersicon

gi19322

0.0051




esculentum


480
G2640

Nicotiana sylvestris

gi121631

0.0061

480
G2640

Physcomitrella

gi21388660

0.01




patens


482
G2649

Brassica oleracea

BH593456

8.00E−72

482
G2649

Hevea brasiliensis

CB376600

3.00E−71

482
G2649

Oryza sativa

AK102444

9.00E−66



( japonica cultivar-



group)

482
G2649

Lycopersicon

AW216650

9.00E−65




esculentum


482
G2649

Oryza sativa

AP004319

4.00E−62

482
G2649
Oryza sativa ( indica
AAAA01008O13

1.00E−61



cultivar-group)

482
G2649

Glycine max

BG155693

3.00E−54

482
G2649

Zea mays

CC628094

1.00E−51

482
G2649

Hordeum vulgare

CA029965

3.00E−44



subsp. vulgare

482
G2649

Medicago

BG645979

3.00E−36




truncatula


482
G2649

Oryza sativa

gi15528818

5.80E−64

482
G2649

Oryza sativa

gi20161862

5.80E−64



( japonica cultivar-



group)

482
G2649

Nicotiana tabacum

gi395147

0.7

482
G2649

Zea mays

gi100922

0.79

482
G2649

Physcomitrella

gi14597654

0.91




patens


482
G2649

Lycopersicon

gi1345532

0.95




esculentum


482
G2649
Oryza sativa ( indica
gi10241429

0.96



cultivar-group)

482
G2649

Chlamydomonas

gi21218057

0.99




reinhardtii


482
G2649

Pisum sativum

gi3426304

1

483
G2650

Glycine max

GLYMA-28NOV01-
1201




CLUSTER427_60

483
G2650

Oryza sativa

OSC2212.C1.p1.fg
1202

483
G2650

Oryza sativa

uC-osrocyp034e01a1
1203

483
G2650

Zea mays

LIB4740-140-A1-K1-E11
1204

483
G2650

Zea mays

ZEAMA-08NOV01-
1205




CLUSTER112147_1

483
G2650

Zea mays

ZEAMA-08NOV01-
1206




CLUSTER112147_3

483
G2650

Zea mays

ZEAMA-08NOV01-
1207




CLUSTER36738_1

483
G2650

Oryza sativa

Os_S23789
1613

483
G2650

Lycopersicon

SGN-UNIGENE-50042
2066




esculentum


483
G2650

Lycopersicon

SGN-UNIGENE-
2067




esculentum

SINGLET-453738

484
G2650

Brassica oleracea

BH937292

2.00E−40

484
G2650

Lotus japonicus

AG245821

9.00E−31

484
G2650

Glycine max

CA799031

3.00E−29

484
G2650

Oryza sativa

AU096089

5.00E−29

484
G2650

Hordeum vulgare

BG301195

8.00E−29

484
G2650

Zea mays

CC637450

1.00E−28

484
G2650

Lactuca sativa

BU003054

1.00E−28

484
G2650

Oryza sativa

GB680333

1.00E−28



( japonica cultivar-



group)

484
G2650
Oryza sativa ( indica
AAAA01007185

1.00E−28



cultivar-group)

484
G2650

Medicago

BF521010

2.00E−28




truncatula


484
G2650

Oryza sativa

gi20804689

2.90E−30



( japonica cultivar-



group)

484
G2650

Lupinus albus

gi20269127

2.00E−17

484
G2650

Oryza sativa

gi14164473

3.50E−17

484
G2650

Lycopersicon

gi12002867

3.90E−17




esculentum


484
G2650

Pueraria montana

gi21624281

4.00E−17



var. lobata

484
G2650

Arundinella hirta

gi13649854

6.10E−13

484
G2650

Linaria vulgaris

gi29788739

6.80E−12

484
G2650

Digitalis purpurea

gi29788741

6.80E−12

484
G2650

Leymus triticoides

gi23307807

1.30E−11

484
G2650

Linaria canadensis

gi29788737

1.50E−11

486
G2655

Oryza sativa

AK107555

4.00E−66



( japonica cultivar-



group)

486
G2655

Brassica oleracea

BH589305

2.00E−64

486
G2655

Lactuca sativa

BQ995023

1.00E−60

486
G2655

Robinia

BI677665

1.00E−52




pseudoacacia


486
G2655

Glycine max

BE021887

1.00E−49

486
G2655

Medicago

CA920255

5.00E−49




truncatula


486
G2655

Zea mays

GC617212

3.00E−47

486
G2655
Oryza sativa ( indica
AAAA01002332

9.00E−47



cultivar-group)

486
G2655

Cicer arietinum

CAR011013

3.00E−42

486
G2655

Arachis hypogaea

CD038481

3.00E−41

486
G2655

Oryza sativa

gi19920107

2.00E−61



( japonica cultivar-



group)

486
G2655

Cucumis melo

gi28558779

1.70E−50

486
G2655

Cicer arietinum

gi3641870

5.00E−42

486
G2655

Zea mays

gi1420924

9.00E−12

486
G2655

Phaseolus vulgaris

gi1142621

2.50E−11

486
G2655

Petunia x hybrida

gi10998404

6.80E−10

486
G2655

Oryza sativa

gi12643064

2.70E−08

486
G2655

Perilla frutescens

gi28375728

7.90E−08

486
G2655

Tripsacum australe

gi527663

8.90E−08

486
G2655

Mesembryanthemum

gi4206118

2.20E−07




crystallinum


487
G2661

Oryza sativa

OSC5501.C1.p4.fg
1208

488
G2661

Brassica oleracea

BH704648

1.00E−29

488
G2661

Sorghum bicolor

BZ341609

1.00E−20

488
G2661

Oryza sativa

AP003252

2.00E−20

488
G2661

Zea mays

CC336314

2.00E−20

488
G2661
Oryza sativa ( indica
AAAA01005031

5.00E−20



cultivar-group)

488
G2661

Hordeum vulgare

BF263465

3.00E−15

488
G2661

Brassica rapa

AT002234

5.00E−15



subsp. pekinensis

488
G2661

Glycine max

CA783614

7.00E−15

488
G2661

Oryza sativa

AK106119

9.00E−15



( japonica cultivar-



group)

488
G2661

Medicago

AC144431

1.00E−14




truncatula


488
G2661

Oryza sativa

gi20160648

1.60E−22



( japonica cultivar-



group)

488
G2661

Oryza sativa

gi15528743

2.50E−16

488
G2661

Tulipa gesneriana

gi5923912

2.70E−07

488
G2661

Pinus taeda

gi6166283

3.50E−07

488
G2661

Petunia x hybrida

gi3127045

4.20E−07

488
G2661

Glycine max

gi3399777

1.10E−05

488
G2661

Perilla frutescens

gi4519199

2.70E−05

488
G2661

Lycopersicon

gi6175252

5.20E−05




esculentum


488
G2661

Antirrhinum majus

gi166428

7.60E−05

488
G2661

Sorghum bicolor

gi527665

0.00017

490
G2679

Glycine max

BM525733

2.00E−71

490
G2679

Lycopersicon

BI421583

2.00E−60




esculentum


490
G2679

Hordeum vulgare

CD054855

4.00E−55

490
G2679

Brassica oleracea

BZ508263

2.00E−53

490
G2679

Triticum aestivum

CA600208

5.00E−52

490
G2679

Beta vulgaris

BQ583502

3.00E−40

490
G2679

Triticum

BQ800694

4.00E−40




monococcum


490
G2679

Medicago

BI308837

7.00E−39




truncatula


490
G2679
Oryza sativa ( indica
CB626016

7.00E−38



cultivar-group)

490
G2679

Oryza sativa

AK071493

2.00E−37



( japonica cultivar-



group)

490
G2679

Oryza sativa

gi14587305

3.10E−33



( japonica cultivar-



group)

490
G2679

Glycine max

gi4218187

2.60E−30

490
G2679

Zea mays

gi20152907

0.0046

490
G2679

Oryza sativa

gi22535907

0.066

490
G2679
Oryza sativa ( indica
gi29565495

0.066



cultivar-group)

490
G2679

Ambrosia trifida

gi114091

0.085

490
G2679

Aster tripolium

gi28804507

0.17

490
G2679

Helianthus

gi18491030

0.39




tuberosus


490
G2679

Casuarina glauca

gi1223652

0.39

490
G2679

Musa acuminata

gi12006148

0.4

492
G2682

Glycine max

BM525733

4.00E−33

492
G2682

Brassica oleracea

BH435758

1.00E−31

492
G2682

Hordeum vulgare

CD054855

2.00E−31

492
G2682

Oryza sativa

AK071493

1.00E−30



( japonica cultivar-



group)

492
G2682

Lycopersicon

BI421583

5.00E−30




esculentum


492
G2682

Triticum aestivum

CA600208

2.00E−26

492
G2682

Zea mays

AY103668

2.00E−24

492
G2682

Medicago

BI308837

1.00E−23




truncatula


492
G2682
Oryza sativa ( indica
CB626016

2.00E−20



cultivar-group)

492
G2682

Zinnia elegans

AU294482

2.00E−20

492
G2682

Glycine max

gi4218187

5.90E−28

492
G2682

Oryza sativa

gi14587305

2.10E−21



( japonica cultivar-



group)

492
G2682

Ambrosia trifida

gi114091

0.03

492
02682

Zea mays

gi20152909

0.037

492
02682

Vigna angularis

gi350610

0.078

492
G2682

Fritillaria agrestis

gi2754855

0.089

492
G2682

Vitis vinifera

gi7406673

0.16

492
G2682

Amburana acreana

gi25453051

0.24

492
G2682

Hordeum vulgare

gi29786353

0.35



subsp. vulgare

492
G2682

Oryza sativa

gi22535907

0.6

494
G2686

Lycopersicon

BG127023

6.00E−21




esculentum


494
G2686

Solanum tuberosum

BE472874

5.00E−20

494
G2686

Glycine max

BQ612521

2.00E−19

494
G2686

Vitis vinifera

CA813725

4.00E−19

494
G2686

Populus tremula x

BU815822

2.00E−18




Populus tremuloides


494
G2686

Nicotiana tabacum

AF193770

4.00E−18

494
G2686

Lactuca sativa

BQ875950

4.00E−17

494
G2686

Brassica oleracea

BH574869

6.00E−17

494
G2686

Capsicum annuum

CA847397

5.00E−16

494
G2686

Medicago

AW560120

5.00E−16




truncatula


494
G2686

Nicotiana tabacum

gi7406995

3.40E−20

494
G2686

Oryza sativa

gi20303630

1.90E−16



( japonica cultivar-



group)

494
G2686

Matricaria

gi17385638

1.60E−15



chamomilla

494
G2686

Oryza sativa

gi15290030

5.60E−14

494
G2686

Petroselinum

gi11493824

2.10E−12




crispum


494
G2686

Solanum tuberosum

gi24745606

6.20E−12

494
G2686

Capsella rubella

gi27817201

9.20E−09

494
G2686

Avena sativa

gi4894963

4.60E−08

494
G2686

Retama raetam

gi18158619

2.40E−07

494
G2686

Solanum dulcamara

gi16588566

4.20E−07

496
G2690

Brassica oleracea

BH516775

1.00E−105

496
G2690

Oryza sativa

AK065008

5.00E−39



( japonica cultivar-



group)

496
G2690

Oryza sativa

AP002913

5.00E−39

496
G2690
Oryza sativa ( indica
AAAA01002497

1.00E−35



cultivar-group)

496
G2690

Helianthus annuus

BQ971511

4.00E−35

496
G2690

Triticum aestivum

BT009310

2.00E−34

496
G2690

Zea mays

CC616336

3.00E−33

496
G2690

Brassica napus

CB686050

3.00E−33

496
G2690

Hordeum vulgare

BU994579

4.00E−28



subsp. vulgare

496
G2690

Gossypium

BQ405698

7.00E−27




arboreum


496
G2690

Oryza sativa

gi12328553

9.90E−39



( japonica cultivar-



group)

496
G2690

Marchantia

gi25272004

9.70E−15




polymorpha


496
G2690

Solanum tuberosum

gi1688233

3.60E−07

496
G2690

Lycopersicon

gi28274830

4.00E−07




esculentum


496
G2690

Hordeum vulgare

gi1730475

5.00E−07



subsp. vulgare

496
G2690

Eragrostis tef

gi17906977

1.10E−06

496
G2690

Atriplex hortensis

gi8571476

1.20E−06

496
G2690

Daucus carota

gi5578746

1.20E−06

496
G2690

Nicotiana tabacum

gi3065895

1.80E−06

496
G2690

Zea mays

gi21908036

1.90E−06

498
G2691

Brassica oleracea

BH591758

7.00E−29

498
G2691

Beta vulgaris

BQ591872

2.00E−15

498
G2691

Lycopersicon

BD194704

1.00E−14




esculentum


498
G2691

Gossypium

BF279235

7.00E−13




arboreum


498
G2691

Nicotiana tabacum

AF058827

7.00E−13

498
G2691

Brassica rapa

BQ791746

2.00E−12



subsp. pekinensis

498
G2691

Brassica napus

BQ704534

3.00E−11

498
G2691

Medicago

AJ503842

9.00E−11




truncatula


498
G2691

Lactuca sativa

BQ873772

9.00E−11

498
G2691

Helianthus

CF091307

1.00E−10




argophyllus


498
G2691

Lycopersicon

gi2213785

5.00E−19




esculentum


498
G2691

Nicotiana tabacum

gi3065895

5.10E−17

498
G2691

Oryza sativa

gi9049421

3.70E−11

498
G2691

Oryza sativa

gi33087061

1.10E−08



( japonica cultivar-



group)

498
G2691

Thellungiella

gi20340233

2.00E−07




halophila


498
G2691

Narcissus

gi18266198

2.50E−07




pseudonarcissus


498
G2691

Solanum tuberosum

gi28268684

4.00E−07

498
G2691

Prunus armeniaca

gi3264767

3.50E−06

498
G2691

Fagus sylvatica

gi18496063

6.60E−06

498
G2691

Zea mays

gi27802487

1.00E−05

500
G2694

Zea mays

BZ743538

4.4

500
G2694

Sorghum bicolor

CD463350

5.8

500
G2694

Oryza sativa

gi32479831

0.04



( japonica cultivar-



group)

502
G2699

Lotus japonicus

AP006085

1.00E−137

502
G2699

Medicago

AC137703

1.00E−132




truncatula


502
G2699

Oryza sativa

AP003823

1.00E−123

502
G2699

Oryza sativa

AP005149

1.00E−123



( japonica cultivar-



group)

502
G2699
Oryza sativa ( indica
AAAA01000614

1.00E−123



cultivar-group)

502
G2699

Brassica oleracea

BH956190

8.00E−65

502
G2699

Zea mays

CC730365

2.00E−62

502
G2699

Solanum tuberosum

BF052580

9.00E−50

502
G2699

Lycopersicon

B0123921

2.00E−45




esculentum


502
G2699

Glycine max

BE473856

2.00E−33

502
G2699

Oryza sativa

gi28564823

4.10E−118



( japonica cultivar-



group)

502
G2699

Lycopersicon

gi13620224

8.30E−33




esculentum


502
G2699

Brassica napus

gi13170126

2.20E−27

502
G2699

Oryza sativa

gi13937306

3.90E−27

502
G2699

Zea mays

gi5640155

4.00E−27

502
G2699

Lilium longiforum

gi32813435

4.80E−27

502
G2699

Capsella rubella

gi13620166

1.10E−26

502
G2699

Triticum aestivum

gi5640157

1.70E−26

502
G2699

Hordeum vulgare

gi18254373

4.30E−26

502
G2699

Dubautia menziesii

gi20257471

2.90E−25

504
G2702

Glycine max

AW234074

3.00E−52

504
G2702

Triticum aestivum

CA730325

8.00E−47

504
G2702

Medicago

BI311137

2.00E−46




truncatula


504
G2702

Oryza sativa

BP184381

3.00E−46



( japonica cultivar-



group)

504
G2702

Oryza sativa

AX699721

5.00E−46

504
G2702

Hordeum vulgare

BU998112

9.00E−46



subsp. vulgare

504
G2702

Lycopersicon

LETHM1

2.00E−45




esculentum


504
G2702

Eschscholzia

CD480267

3.00E−45




californica


504
G2702

Sorghum bicolor

CD226268

6.00E−45

504
G2702

Triphysaria

BM356984

6.00E−45




versicolor


504
G2702

Oryza sativa

gi20146436

7.50E−48



( japonica cultivar-



group)

504
G2702

Oryza sativa

gi13486737

2.00E−47

504
G2702

Lycopersicon

gi1167486

7.70E−46




esculentum


504
G2702
Dendrobium sp.
gi28628949

6.20E−44



XMW-2002-2

504
G2702

Hordeum vulgare

gi19059

1.60E−43

504
G2702

Gossypium

gi13346194

2.90E−43




hirsutum


504
G2702

Nicotiana tabacum

gi6552389

1.20E−40

504
G2702

Fragaria x

gi15082210

2.50E−40




ananassa


504
G2702

Antirrhinum majus

gi82310

4.40E−40

504
G2702

Petunia x hybrida

gi20561

5.20E−40

505
G2717

Glycine max

GLYMA-28NOV01-
1209




CLUSTER16793_1

505
G2717

Glycine max

GLYMA-28NOV01-
1210





CLUSTER16793_3

505
G2717

Glycine max

GLYMA-28NOV01-
1211




CLUSTER23207_1

505
G2717

Glycine max

GLYMA-28NOV01-
1212




CLUSTER30577_1

505
G2717

Glycine max

GLYMA-28NOV01-
1213




CLUSTER30577_4

505
G2717

Glycine max

LIB3053-003-Q1-N1-A7
1214

505
G2717

Glycine max

jC-gmle01210024c12a1
1215

505
G2717

Oryza sativa

AU075998.1
1216

505
G2717

Oryza sativa

ORYSA-22JAN02-
1217




CLUSTER275001_1

505
G2717

Oryza sativa

ORYSA-22JAN02-
1218




CLUSTER62825_1

505
G2717

Oryza sativa

OSC100863.C1.p7.fg
1219

505
G2717

Oryza sativa

OSC17223.C1.p2.fg
1220

505
G2717

Oryza sativa

OSC21325.C1.p9.fg
1221

505
G2717

Zea mays

LIB3689-236-Q1-K6-H9
1222

505
G2717

Zea mays

LIB4758-055-R2-K1-
1223




G11

505
G2717

Zea mays

ZEAMA-08NOV01-
1224




CLUSTER25294_1

505
G2717

Zea mays

ZEAMA-08NOV01-
1225




CLUSTER25294_2

505
G2717

Zea mays

ZEAMA-08NOV01-
1226




CLUSTER304_164

505
G2717

Zea mays

ZEAMA-08NOV01-
1227




CLUSTER304_172

505
G2717

Oryza sativa

Os_S96374
1614

505
G2717

Glycine max

Gma_S4993926
1670

505
G2717

Hordeum vulgare

Hv_S134310
1744

505
G2717

Zea mays

Zm_S11527070
1818

505
G2717

Triticum aestivum

Ta_S167441
1907

505
G2717

Triticum aestivum

Ta_S275432
1908

505
G2717

Triticum aestivum

Ta_S88094
1909

505
G2717

Lycopersicon

SGN-UNIGENE-51988
2068




esculentum


505
G2717

Lycopersicon

SGN-UNIGENE-
2069




esculentum

SINGLET-393701

506
G2717

Brassica napus

CD814949

7.00E−90

506
G2717

Oryza sativa

AK100618

2.00E−74



( japonica cultivar-



group)

506
G2717

Lycopersicon

BG127613

3.00E−65




esculentum


506
G2717

Vitis vinifera

GB007263

5.00E−63

506
G2717

Ipomoea nil

BJ563043

2.00E−61

506
G2717

Medicago

BF003720

3.00E−61




truncatula


506
G2717

Pennisetum ciliare

BM084769

2.00E−58

506
G2717

Glycine max

BU964889

3.00E−55

506
G2717

Solanum tuberosum

BQ119267

2.00E−54

506
G2717

Hordeum vulgare

BM816006

4.00E−52

506
G2717

Oryza sativa

gi20146249

1.10E−69



( japonica cultivar-



group)

506
G2717

Zea mays

gi18463961

1.80E−39

506
G2717

Petroselinum

gi2224899

7.80E−21




crispum


506
G2717

Nicotiana tabacum

gi1084419

2.20E−14

506
G2717

Triticum aestivum

gi283024

5.10E−14

506
G2717

Fritillaria liliacea

gi15281590

1.10E−13

506
G2717

Lycopersicon

gi3021487

5.40E−13




esculentum


506
G2717

Fritillaria agrestis

gi2641211

2.70E−12

506
G2717

Medicago

gi32966575

3.10E−11




truncatula


506
G2717

Lens culinaris

gi13540405

6.10E−11

507
G2718

Glycine max

GLYMA-28NOV01-
1057




CLUSTER31802_1

507
G2718

Glycine max

GLYMA-28NOV01-
1058




CLUSTER586_102

507
G2718

Glycine max

GLYMA-28NOV01-
1059




CLUSTER586_116

507
G2718

Glycine max

GLYMA-28NOV01-
1060




CLUSTER8724_1

507
G2718

Glycine max

GLYMA-28NOV01-
1061




CLUSTER8724_2

507
G2718

Oryza sativa

ORYSA-22JAN02-
1063




CLUSTER30974_3

507
G2718

Oryza sativa

OSC20053.C1.p5.fg
1064

507
G2718

Oryza sativa

OSC20055.C1.p5.fg
1065

507
G2718

Zea mays

ZEAMA-08NOV01-
1066




CLUSTER69699_1

507
G2718

Zea mays

ZEAMA-08NOV01-
1067




CLUSTER69699_2

507
G2718

Glycine max

Gma_S4901946
1663

507
G2718

Triticum aestivum

Ta_S45274
1883

508
G2718

Brassica oleracea

BH961028

1.00E−24

508
G2718

Populus tremula x

BU831849

3.00E−21




Populus tremuloides


508
G2718

Populus

BU872107

3.00E−21



balsamifera subsp.




trichocarpa


508
G2718

Vitis vinifera

BM437313

6.00E−20

508
G2718

Vitis aestivalis

CB289238

3.00E−19

508
G2718

Glycine max

BI699876

2.00E−18

508
G2718

Pinus pinaster

AL750151

4.00E−16

508
G2718

Hordeum vulgare

AV911235

1.00E−12



subsp. vulgare

508
G2718

Nuphar advena

CD473522

2.00E−12

508
G2718

Oryza sativa

CB684618

4.00E−12



( japonica cultivar-



group)

508
G2718

Solanum tuberosum

gi9954118

7.20E−11

508
G2718

Vitis labrusca x

gi22266671

3.10E−10




Vitis vinifera


508
G2718

Gossypium

gi23476287

3.10E−10




hirsutum


508
G2718

Gossypium

gi23476291

3.10E−10




raimondii


508
G2718

Gossypium

gi23476293

3.10E−10




herbaceum


508
G2718

Gossypioides kirkii

gi23476295

3.10E−10

508
G2718

Fragaria x

gi15082210

5.00E−10




ananassa


508
G2718

Oryza sativa

gi19072770

6.40E−10

508
G2718

Zea luxurians

gi15042120

8.20E−10

508
G2718

Zea mays

gi19548449

8.20E−10

509
G2723

Oryza sativa

rsicek_8958.y1.abd
1228

510
G2723

Brassica rapa

L38243

8.00E−40

510
G2723

Vitis vinifera

CB920052

1.00E−26

510
G2723

Populus tremula

BU890694

2.00E−26

510
G2723

Phaseolus

CA897021

6.00E−25




coccineus


510
G2723

Eucalyptus grandis

CD669972

3.00E−24

510
G2723

Glycine max

B0726181

5.00E−24

510
G2723

Lycopersicon

AJ320067

3.00E−23




esculentum


510
G2723

Lotus japonicus

AP004546

3.00E−23

510
G2723

Solanum tuberosum

BI433702

7.00E−23

510
G2723

Capsicum annuum

BM060888

6.00E−22

510
G2723

Lycopersicon

gi7981380

2.50E−24




esculentum


510
G2723

Oryza sativa

gi20161824

1.10E−21



( japonica cultivar-



group)

510
G2723

Oryza sativa

gi5091605

3.80E−21

510
G2723

Hevea brasiliensis

gi12005328

1.20E−14

510
G2723

Antirrhinum majus

gi18874265

7.30E−14

510
G2723

Glycine max

gi19911577

5.40E−13

510
G2723

Solanum demissum

gi15209176

8.30E−13

510
G2723

Lilium longiflorum

gi31442292

0.0034

510
G2723

Volvox carteri f.

gi4633127

0.012




nagariensis


510
G2723
Oryza sativa ( indica
gi10443488

0.64



cultivar-group)

511
G2741

Glycine max

BG508638.1
1229

511
G2741

Glycine max

GLYMA-28NOV01-
1230




CLUSTER5654_1

511
G2741

Glycine max

GLYMA-28NOV01-
1231




CLUSTER5654_2

511
G2741

Oryza sativa

OSC102289.C1.p18.fg
1232

511
G2741

Oryza sativa

OSC5384.C1.p5.fg
1233

511
G2741

Oryza sativa

rsicen_25533.y1.abd
1234

511
G2741

Oryza sativa

rsicen_8566.y1.abd
1235

511
G2741

Zea mays

ZEAMA-08NOV01-
1236




CLUSTER73638_1

511
G2741

Glycine max

Gma_S4922181
1671

511
G2741

Hordeum vulgare

Hv_S24580
1745

511
G2741

Zea mays

Zm_S11434269
1819

511
G2741

Lycopersicon

SGN-UNIGENE-50878
2070




esculentum


511
G2741

Lycopersicon

SGN-UNIGENE-
2071




esculentum

SINGLET-356106

512
G2741

Oryza sativa

AP003277

2.00E−56

512
G2741

Brassica oleracea

BZ506408

6.00E−48

512
G2741

Zea mays

BZ709707

1.00E−47

512
G2741

Glycine max

CA953428

4.00E−45

512
G2741

Lycopersicon

BE432293

3.00E−39




esculentum


512
G2741

Oryza sativa

AC130607

7.00E−39



( japonica cultivar-



group)

512
G2741

Hordeum vulgare

BE559431

2.00E−37

512
G2741

Oryza minuta

CR210034

2.00E−34

512
G2741
Oryza sativa ( indica
AAAA01011300

5.00E−34



cultivar-group)

512
G2741

Lactuca sativa

BU000462

1.00E−33

512
G2741

Oryza sativa

gi15289981

3.20E−57

512
G2741

Oryza sativa

gi20160613

9.30E−29



( japonica cultivar-



group)

512
G2741

Zea mays

gi13661174

3.00E−25

512
G2741

Oryza glaberrima

gi31338862

2.50E−13

512
G2741
Oryza sativa ( indica
gi31338860

7.60E−13



cultivar-group)

512
G2741

Chlamydomonas

gi5916207

3.20E−11




reinhardtii


512
G2741

Mesembryanthemum

gi6942190

7.90E−11




crystallinum


512
G2741

Nicotiana tabacum

gi4519671

1.20E−09

512
G2741

Solanum

gi32470629

4.30E−09




bulbocastanum


512
G2741

Pisum sativum

gi23504755

0.063

514
G2743

Medicago

CF068634

3.00E−50




truncatula


514
G2743

Oryza sativa

AK064355

2.00E−49



( japonica cultivar-



group)

514
G2743

Solanum tuberosum

BQ513305

2.00E−40

514
G2743

Lycopersicon

AW398166

7.00E−40




pennellii


514
G2743

Populus tremuloides

CA926221

4.00E−38

514
G2743

Brassica oleracea

BH485910

9.00E−37

514
G2743

Lycopersicon

BE450553

3.00E−36




esculentum


514
G2743

Glycine max

BM524732

3.00E−33

514
G2743

Hordeum vulgare

BU988945

1.00E−30



subsp. vulgare

514
G2743

Prunus armeniaca

CR820349

4.00E−27

514
G2743

Oryza sativa

gi11034542

1.50E−49

514
G2743

Oryza sativa

gi31415946

4.50E−41



( japonica cultivar-



group)

514
G2743

Zea mays

gi14189890

4.30E−12

514
G2743

Chlamydomonas

gi5916207

5.00E−12




reinhardtii


514
G2743

Mesembryanthemum

gi6942190

1.80E−10




crystallinum


514
G2743

Solanum

gi32470629

2.40E−09




bulbocastanum


514
G2743

Nicotiana tabacum

gi4519671

4.80E−09

514
G2743

Oryza glaberrima

gi31338862

1.30E−08

514
G2743
Oryza sativa ( indica
gi31338860

8.10E−08



cultivar-group)

514
G2743

Hordeum vulgare

gi12406993

0.079

516
G2747

Brassica oleracea

BH542430

1.00E−110

516
G2747
Oryza sativa ( indica
AAAA01011764

1.00E−51



cultivar-group)

516
G2747

Lycopersicon

BI934304

3.00E−51




esculentum


516
G2747

Oryza sativa

AX654655

7.00E−51

516
G2747

Oryza sativa

AK106367

3.00E−50



( japonica cultivar-



group)

516
G2747

Lactuca sativa

BU005803

1.00E−49

516
G2747

Zea mays

AG144718

9.00E−44

516
G2747

Solanum tuberosum

BQ119486

1.00E−43

516
G2747

Capsicum annuum

BM063508

1.00E−42

516
G2747

Glycine max

AW760132

3.00E−39

516
G2747

Oryza sativa

gi21426118

2.80E−52



( japonica cultivar-



group)

516
G2747

Marchantia

gi25272004

7.10E−39




polymorpha


516
G2747

Oryza sativa

gi19352041

2.30E−09

516
G2747

Prunus persica

gi27450533

7.10E−09

516
G2747

Mangifera indica

gi31747324

3.60E−08

516
G2747

Eragrostis tef

gi17906977

7.70E−08

516
G2747

Hordeum vulgare

gi1730475

1.00E−07



subsp. vulgare

516
G2747

Phaseolus vulgaris

gi1046278

9.40E−07

516
G2747

Pisum sativum

gi22335711

1.20E−06

516
G2747

Zea mays

gi100922

4.00E−06

517
G2754

Glycine max

GLYMA-28NOV01-
1237




CLUSTER34285_1

517
G2754

Glycine max

LIB4167-054-R1-K1-B3
1238

517
G2754

Oryza sativa

AU062733.2
1239

517
G2754

Oryza sativa

LIB4309-004-Q1-K1-B9
1240

517
G2754

Oryza sativa

OSC13803.C1.p1.fg
1241

517
G2754

Zea mays

ZEAMA-08NOV01-
1242




CLUSTER33736_2

517
G2754

Zea mays

ZEAMA-08NOV01-
1243




CLUSTER41889_1

517
G2754

Oryza sativa

Os_S24071
1615

517
G2754

Hordeum vulgare

Hv_S103555
1746

517
G2754

Zea mays

Zm_S11456823
1820

517
G2754

Zea mays

Zm_S11521388
1821

517
G2754

Zea mays

Zm_S11523999
1822

517
G2754

Triticum aestivum

Ta_S210098
1910

517
G2754

Triticum aestivum

Ta_S272733
1911

518
G2754

Zea mays

AF461813

1.0e−999

518
G2754

Oryza sativa

AK100732

1.00E−128



( japonica cultivar-



group)

518
G2754

Chenopodium

AX652119

1.00E−111




amaranticolor


518
G2754
Oryza sativa ( indica
AAAA01000051

2.00E−92



cultivar-group)

518
G2754

Triticum aestivum

GA499811

6.00E−90

518
G2754

Hordeum vulgare

CB882737

4.00E−86



subsp. vulgare

518
G2754

Lactuca sativa

BU006379

7.00E−86

518
G2754

Oryza sativa

AP004006

6.00E−84

518
G2754

Medicago

BQ122721

1.00E−83




truncatula


518
G2754

Nicotiana tabacum

BP129768

6.00E−77

518
G2754

Zea mays

gi18463957

3.30E−251

518
G2754

Oryza sativa

gi12083522

1.70E−132

518
G2754

Oryza sativa

gi21952816

5.30E−86



( japonica cultivar-



group)

518
G2754

Hordeum vulgare

gi23193479

1.20E−83



subsp. vulgare

516
G2754

Hordeum vulgare

gi23193481

5.60E−82

518
G2754

Triticum

gi23193487

1.20E−81




monococcum


518
G2754

Glycine max

gi25172762

3.30E−15

518
G2754

Nicotiana tabacum

gi8096269

0.54

518
G2754

Lycopersicon

gi4731573

0.87




esculentum


518
G2754

Alnus glutinosa

gi2467082

0.9

520
G2757

Brassica napus

CD833979

1.00E−109

520
G2757

Brassica oleracea

BH475285

3.00E−85

520
G2757

Glycine max

AI494758

4.00E−29

520
G2757

Medicago

CB891664

2.00E−27




truncatula


520
G2757

Ipomoea nil

BJ553668

4.00E−26

520
G2757

Oryza sativa

AC084763

1.00E−25

520
G2757

Oryza sativa

AE017120

1.00E−25



( japonica cultivar-



group)

520
G2757
Oryza sativa ( indica
AAAA01000681

1.00E−25



cultivar-group)

520
G2757

Zea mays

BZ420052

5.00E−25

520
G2757

Lycopersicon

BG132323

1.00E−24




esculentum


520
G2757

Oryza sativa

gi12597883

3.90E−39

520
G2757

Oryza sativa

gi31433545

3.90E−39



( japonica cultivar-



group)

520
G2757

Nicotiana tabacum

gi18149189

1.50E−14

520
G2757

Zea mays

gi5731354

0.97

520
G2757

Glycine max

gi18182311

0.99

520
G2757

Zinnia elegans

gi531098

0.99

520
G2757

Chloroplast

gi21309708

1




Phaseolus vulgaris


520
G2757
Phalaenopsis sp.
gi1173628

1



SM9108

520
G2757

Ranunculus ficaria

gi27995073

1

520
G2757

Cucumis melo

gi7595346

1

521
G2763

Hordeum vulgare

Hv_S114962
1747

522
G2763

Brassica oleracea

BH560418

3.00E−75

522
G2763

Thellungiella

BM986046

3.00E−73




halophila


522
G2763

Poncirus trifoliata

CD575942

3.00E−54

522
G2763

Populus tremula x

BU896429

2.00E−50




Populus tremuloides


522
G2763

Ipomoea nil

BJ574783

1.00E−48

522
G2763

Solanum tuberosum

BG351877

2.00E−47

522
G2763

Glycine max

CD415130

8.00E−43

522
G2763

Lycopersicon

BM409924

9.00E−42




esculentum


522
G2763

Medicago

AC135100

9.00E−42




truncatula


522
G2763

Theobroma cacao

CA797288

4.00E−41

522
G2763

Oryza sativa

gi24059889

4.80E−21



( japonica cultivar-



group)

522
G2763

Tulipa gesneriana

gi5923912

0.03

522
G2763

Ananas comosus

gi25044805

0.034

522
G2763

Oryza sativa

gi5852091

0.29

522
G2763

Oryza

gi1086530

0.66



longistaminata

522
G2763

Zea mays

gi1244653

0.76

522
G2763

Oryza rufipogon

gi1086536

0.85

522
G2763
Quercus robur
gi33111961

0.98

522
G2763

Triticum

gi30090030

1




monococcum


522
G2763

Glycine max

gi3399777

1

524
G2765

Oryza sativa

AK106649

4.00E−61



( japonica cultivar-



group)

524
G2765

Lycopersicon

AI488313

5.00E−60




esculentum


524
G2765

Brassica oleracea

BH582059

4.00E−51

524
G2765

Glycine max

BE020519

2.00E−50

524
G2765
Oryza sativa subsp.
AU093196

4.00E−49




japonica


524
G2765

Populus tremula x

BU813371

1.00E−37




Populus tremuloides


524
G2765

Medicago

BF647687

2.00E−37




truncatula


524
G2765

Pinus pinaster

BX252556

1.00E−32

524
G2765

Populus

BU869748

4.00E−32



balsamifera subsp.




trichocarpa


524
G2765

Zea mays

BZ644709

3.00E−31

524
G2765

Oryza sativa

gi32129332

2.30E−30



( japonica cultivar-



group)

524
G2765

Oryza sativa

gi10800070

3.80E−28

524
G2765

Pennisetum

gi527655

8.40E−09




glaucum


524
G2765

Perilla frutescens

gi28375728

1.30E−08

524
G2765

Sorghum bicolor

gi527665

1.40E−08

524
G2765

Oryza australiensis

gi1086526

1.80E−08

524
G2765

Oryza rufipogon

gi1086536

2.30E−08

524
G2765

Phyllostachys acuta

gi527661

3.80E−08

524
G2765

Oryza

gi1086530

4.90E−08




longistaminata


524
G2765

Oryza officinalis

gi1086534

1.00E−07

525
G2768

Glycine max

GLYMA-28NOV01-
1244




CLUSTER48270_1

525
G2768

Glycine max

GLYMA-28NOV01-
1245




CLUSTER48270_2

525
G2768

Glycine max

GLYMA-28NOV01-
1246




CLUSTER48270_6

525
G2768

Glycine max

GLYMA-28NOV01-
1247




CLUSTER777019_1

525
G2768

Glycine max

LIB4165-033-Q1-K1-E7
1248

525
G2768

Glycine max

jC-gmle01810065e09d1
1249

525
G2768

Oryza sativa

ORYSA-22JAN02-
1250




CLUSTER22_6

525
G2768

Oryza sativa

ORYSA-22JAN02-
1251




CLUSTER22_7

525
G2768

Oryza sativa

OSC10851.C1.p4.fg
1252

525
G2768

Oryza sativa

OSC19157.C1.p4.fg
1253

525
G2768

Oryza saziva

OSC32936.C1.p1.fg
1254

525
G2768

Zea mays

ZEAMA-08NOV01-
1255




CLUSTER21189_1

525
G2768

Zea mays

ZEAMA-08NOV01-
1256




CLUSTER21189_3

525
G2768

Zea mays

ZEAMA-08NOV01-
1257




CLUSTER35998_1

525
G2768

Zea mays

ZEAMA-08NOV01-
1258




CLUSTER692790_1

525
G2768

Zea mays

ZEAMA-08NOV01-
1259




CLUSTER697_56

525
G2768

Zea mays

ZEAMA-08NOV01-
1260




CLUSTER697_59

525
G2768

Zea mays

ZEAMA-08NOV01-
1261




CLUSTER697_60

525
G2768

Hordeum vulgare

Hv_S30775
1748

525
G2768

Triticum aestivum

Ta_S127458
1912

525
G2768

Lycopersicon

SGN-UNIGENE-49806
2072




esculentum


525
G2768

Lycopersicon

SGN-UNIGENE-51288
2073




esculentum


525
G2768

Lycopersicon

SGN-UNIGENE-
2074




esculentum

SINGLET-13430

526
G2768

Oryza sativa

AK106568

1.00E−146



( japonica cultivar-



group)

526
G2768

Beta vulgaris

BQ589936

7.00E−72

526
G2768

Brassica oleracea

BZ518223

2.00E−64

526
G2768

Medicago

AC119408

2.00E−56




truncatula


526
G2768

Hordeum vulgare

BQ758728

2.00E−56



subsp. vulgare

526
G2768

Sorghum bicolor

CD222928

9.00E−56

526
G2768

Lycopersicon

BI931469

3.00E−53




esculentum


526
G2768

Oryza sativa

AC087545

2.00E−49

526
G2768
Oryza sativa ( indica
AAAA01000530

2.00E−49



cultivar-group)

526
G2768

Ceratopteris

BE640922

1.00E−35




richardii


526
G2768

Oryza sativa

gi31432245

9.90E−155



( japonica cultivar-



group)

526
G2768

Oryza sativa

gi14028993

2.00E−08

526
G2768

Nicotiana

gi3850823

1.50E−07



plumbaginifolia

526
G2768

Lycopersicon

gi9858779

3.70E−06




esculentum


526
G2768

Cucurbita maxima

gi17221648

1.10E−05

526
G2768

Ipomoea nil

gi11127996

3.80E−05

526
G2768
Rosa hybrid cultivar-
gi15029364

0.0001

526
G2768

Pisum sativum

gi7688063

0.00022

526
G2768

Hordeum vulgare

gi520943

0.00053



subsp. vulgare

526
G2768

Hordeum vulgare

gi629788

0.00053

528
G2771

Glycine max

GA783908

1.00E−35

528
G2771

Brassica oleracea

BZ510921

3.00E−33

528
G2771

Lycopersicon

BE431510

1.00E−30




esculentum


528
G2771

Amborella

CD482068

1.00E−29




trichopoda


528
G2771

Prunus dulcis

BU574318

2.00E−29

528
G2771

Oryza sativa

AK060505

6.00E−28



( japonica cultivar-



group)

528
G2771

Solanum tuberosum

BG591063

4.00E−27

528
G2771

Populus tremula x

BU866069

4.00E−27




Populus tremuloides


528
G2771

Oryza minuta

CB209966

1.00E−26

528
G2771

Sorghum bicolor

BE598711

2.00E−26

528
G2771

Oryza sativa

gi31043851

6.10E−27

528
G2771

Oryza sativa

gi23495742

3.10E−25



( japonica cultivar-



group)

528
G2771

Tulipa gesneriana

gi5923912

1.40E−14

528
G2771

Oryza rufipogon

gi1086538

1.70E−07

528
G2771

Pennisetum

gi527653

1.70E−07




glaucum


528
G2771

Phyllostachys acuta

gi527661

1.70E−07

528
G2771

Sorghum bicolor

gi527665

1.70E−07

528
G2771

Gossypium

gi13346180

1.10E−06




hirsutum


528
G2771

Zea mays

gi100921

1.20E−06

528
G2771

Oryza officinalis

gi1086534

1.20E−06

529
G2776

Glycine max

GLYMA-28NOV01-
1262




CLUSTER10667_1

529
G2776

Glycine max

GLYMA-28NOV01-
1263




CLUSTER249_1

529
G2776

Glycine max

GLYMA-28NOV01-
1264




CLUSTER27648_1

529
G2776

Glycine max

GLYMA-28NOV01-
1265




CLUSTER318_3

529
G2776

Glycine max

GLYMA-28NOV01-
1266




CLUSTER318_5

529
G2776

Glycine max

GLYMA-28NOV01-
1267




CLUSTER362284_1

529
G2776

Glycine max

GLYMA-28NOV01-
1268




CLUSTER38253_1

529
G2776

Glycine max

GLYMA-28NOV01-
1269




CLUSTER92523_2

529
G2776

Glycine max

GLYMA-28NOV01-
1270




CLUSTER92523_4

529
G2776

Oryza sativa

ORYSA-22JAN02-
1271




CLUSTER278119_1

529
G2776

Oryza sativa

OSC21904.C1.p3.fg
1272

529
G2776

Oryza sativa

OSC22826.C1.p11.fg
1273

529
G2776

Oryza sativa

OSC6897.C1.p1.fg
1274

529
G2776

Oryza sativa

OSC9960.C1.p2.fg
1275

529
G2776

Oryza sativa

OSC9961.C1.p3.fg
1276

529
G2776

Zea mays

ZEAMA-08NOV01-
1277




CLUSTER607309_1

529
G2776

Zea mays

ZEAMA-08NOV01-
1278




CLUSTER81320_1

529
G2776

Glycine max

Gma_S4925755
1672

529
G2776

Glycine max

Gma_S5146563
1673

529
G2776

Triticum aestivum

Ta_S121078
1913

529
G2776

Lycopersicon

SGN-UNIGENE-49159
2075




esculentum


529
G2776

Lycopersicon

SGN-UNIGENE-
2076




esculentum

SINGLET-357057

530
G2776

Brassica oleracea

BZ437288

5.00E−48

530
G2776

Glycine max

AF069738

2.00E−47

530
G2776

Lotus japonicus

AI967554

4.00E−46

530
G2776

Lycopersicon

AI896266

1.00E−45




esculentum


530
G2776

Populus tremula x

BU884552

4.00E−43




Populus tremuloides


530
G2776

Medicago

AW775712

4.00E−40




truncatula


530
G2776

Mesembryanthemum

AF097665

2.00E−38




crystallinum


530
G2776

Oryza sativa

AC145380

2.00E−33



( japonica cultivar-



group)

530
G2776
Oryza sativa ( indica
AAAA01000416

2.00E−33



cultivar-group)

530
G2776

Triticum aestivum

BQ483543

2.00E−30

530
G2776

Glycine max

gi3399777

1.40E−48

530
G2776

Mesembryanthemum

gi4206118

7.40E−41




crystallinum


530
G2776

Oryza sativa

gi29788848

4.70E−33



( japonica cultivar-



group)

530
G2776

Oryza sativa

gi18542931

6.60E−24

530
G2776

Phaseolus vulgaris

gi1142619

6.10E−19

530
G2776

Lycopersicon

gi6175252

5.80E−16




esculentum


530
G2776

Zea mays

gi4321762

4.70E−15

530
G2776

Petunia x hybrida

gi10998404

2.30E−14

530
G2776

Pennisetum

gi527653

2.80E−12




glaucum


530
G2776

Phyllostachys acuta

gi527661

7.80E−12

532
G2777

Medicago

BF521311

1.00E−64




truncatula


532
G2777

Brassica napus

CD813382

1.00E−64

532
G2777

Citrus sinensis

CB293518

6.00E−56

532
G2777

Oryza sativa

AK071315

2.00E−53



( japonica cultivar-



group)

532
G2777

Glycine max

AW832440

5.00E−53

532
G2777

Lactuca sativa

BQ870016

1.00E−52

532
G2777

Brassica rapa

BG544499

3.00E−52



subsp. pekinensis

532
G2777

Populus tremula x

BU831324

4.00E−40




Populus tremuloides


532
G2777

Brassica oleracea

BZ432234

4.00E−38

532
G2777

Lycopersicon

BI926824

5.00E−37




esculentum


532
G2777

Phyllostachys acuta

gi527661

1.20E−06

532
G2777

Pennisetum

gi527655

2.00E−06




glaucum


532
G2777

Lycopersicon

gi23600383

2.10E−06




esculentum


532
G2777

Sorghum bicolor

gi527665

5.30E−06

532
G2777

Oryza sativa

gi22758263

6.10E−06



( japonica cultivar-



group)

532
G2777

Tripsacum australe

gi527663

6.80E−06

532
G2777

Phaseolus vulgaris

gi1142621

8.60E−06

532
G2777

Oryza rufipogon

gi1086536

8.70E−06

532
G2777

Oryza australiensis

gi1086526

1.80E−05

532
G2777

Oryza sativa

gi15451582

2.10E−05

534
G2779

Glycine max

BE347561

4.00E−47

534
G2779

Populus tremula x

BU811904

2.00E−43




Populus tremuloides


534
G2779

Medicago

CB066613

1.00E−29




truncatula


534
G2779

Oryza sativa

AK059041

2.00E−29



( japonica cultivar-



group)

534
G2779

Triticum aestivum

BJ211785

5.00E−29

534
G2779

Zea mays

CB604124

1.00E−28

534
G2779

Lycopersicon

AI490119

3.00E−28




esculentum


534
G2779

Lotus corniculatus

GB828026

3.00E−28



var. japonicus

534
G2779

Hordeum vulgare

GA003238

7.00E−28



subsp. vulgare

534
G2779

Capsicum annuum

CA522636

7.00E−28

534
G2779

Oryza sativa

gi20804997

1.20E−30



( japonica cultivar-



group)

534
G2779

Tulipa gesneriana

gi5923912

2.30E−30

534
G2779

Oryza sativa

gi11862964

7.90E−28

534
G2779

Pinus taeda

gi6166283

4.20E−10

534
G2779

Glycine max

gi3399777

3.00E−05

534
G2779

Gossypium

gi13346182

5.30E−05




hirsutum


534
G2779

Sorghum bicolor

gi527665

0.00018

534
G2779

Brassica napus

gi27650307

0.00023

534
G2779

Petunia x hybrida

gi10998404

0.0005

534
G2779

Phaseolus vulgaris

gi1142621

0.00071

536
G2783

Nicotiana

NPL292767

1.00E−127



plumbaginifoia

536
G2783

Zea mays

AY107267

1.00E−125

536
G2783

Oryza sativa

AK101593

1.00E−124



( japonica cultivar-



group)

536
G2783

Gossypium

CA993585

1.00E−113




hirsutum


536
G2783

Beta vulgaris

BVU313097

1.00E−110

536
02783

Medicago

CB893695

1.00E−104




truncatula


536
G2783

Triticum aestivum

BT009299

1.00E−102

536
G2783

Nicotiana tabacum

AF029351

3.00E−97

536
G2783

Glycine max

CA784546

1.00E−96

536
G2783

Gossypium

BF278029

8.00E−94




arboreum


536
G2783

Nicotiana

gi9663767

9.00E−123



plumbaginifolia

536
G2783

Oryza sativa

gi12583812

2.50E−120

536
G2783

Oryza sativa

gi32488785

2.50E−120



( japonica cultivar-



group)

536
G2783

Beta vulgaris

gi30524689

1.60E−107

536
G2783

Nicotiana tabacum

gi2708532

3.70E−94

536
G2783

Sorghum bicolor

gi22208507

2.70E−28

536
G2783

Zea mays

gi23928438

5.10E−25

536
G2783

Solanum tuberosum

gi17432522

7.90E−22

536
G2783

Nicotiana sylvestris

gi100293

5.40E−19

536
G2783

Cucumis sativus

gi7528270

6.70E−18

537
02784

Glycine max

Gma_S5128871
1674

537
G2784

Lycopersicon

SGN-UNIGENE-
2077




esculentum

SINGLET-68213

538
G2784

Brassica oleracea

BH966813

6.00E−65

538
G2784

Mesembryanthemum

BE034373

2.00E−62




crystallinum


538
G2784

Glycine max

BE659785

2.00E−60

538
G2784

Populus tremula

BU821418

1.00E−42

538
G2784

Lycopersicon

AW218420

2.00E−35




esculentum


538
G2784

Solanum tuberosum

BQ115041

2.00E−31

538
G2784

Oryza sativa

OSJN00052

8.00E−31

538
G2784

Zea mays

CC656270

8.00E−29

538
G2784

Pinus taeda

BFS16719

6.00E−27

538
G2784

Medicago

BG589047

2.00E−26




truncatula


538
G2784

Oryza sativa

gi21740764

4.30E−68

538
G2784

Oryza sativa

gi31433498

8.30E−30



( japonica cultivar-



group)

538
G2784

Chlamydomonas

gi18137

0.36




reinhardtii


538
G2784

Lycopersicon

gi170408

0.96




esculentum


538
G2784

Physcomitrella

gi11181645

0.98




patens


538
G2784

Nicotiana tabacum

gi237857

1

540
G2790

Brassica oleracea

BH660922

1.00E−35

540
G2790

Medicago

BQ124408

5.00E−27




truncatula


540
G2790

Oryza sativa

AK072833

6.00E−27



( japonica cultivar-



group)

540
G2790

Populus tremula x

BU895329

9.00E−26




Populus tremuloides


540
G2790

Gossypium

BG440718

1.00E−25




arboreum


540
G2790

Glycine max

BG046947

2.00E−25

540
G2790

Solanum tuberosum

BQ514720

4.00E−25

540
G2790

Gossypium

CA993210

1.00E−24




hirsutum


540
G2790

Lycopersicon

AF096263

2.00E−24




esculentum


540
G2790

Populus

AI166770

4.00E−24



balsamifera subsp.




trichocarpa


540
G2790

Oryza sativa

gi14488370

8.00E−28

540
G2790

Brassica napus

gi11045087

3.10E−26

540
G2790

Lycopersicon

gi5669656

1.00E−25




esculentum


540
G2790

Oryza sativa

gi32480231

2.20E−25



( japonica cultivar-



group)

540
G2790

Glycine max

gi3399777

0.0012

540
G2790
Pennisetum
gi527657

0.002




glaucum


540
G2790

Tulipa gesneriana

gi5923912

0.004

540
G2790

Zea mays

gi18568238

0.009

540
G2790

Phaseolus vulgaris

gi1142621

0.022

540
G2790

Oryza rufipogon

gi1086538

0.037

542
G2802

Oryza sativa

AK106152

1.00E−132



( japonica cultivar-



group)

542
G2802
Oryza sativa ( indica
CB630225

1.00E−102



cultivar-group)

542
G2802

Medicago

BQ148509

7.00E−98




truncatula


542
G2802

Pinus pinaster

BX251486

7.00E−84

542
G2802

Hordeum vulgare

BI960052

1.00E−77

542
G2802

Brassica oleracea

BH656772

3.00E−76

542
G2802

Populus tremula x

BI129724

3.00E−76




Populus tremuloides


542
G2802

Pinus taeda

BF518231

9.00E−74

542
G2802

Glycine max

BM527360

1.00E−71

542
G2802

Triticum aestivum

BJ315410

6.00E−67

542
G2802

Oryza sativa

gi9049470

2.30E−85

542
G2802

Oryza sativa

gi18461166

3.50E−78



( japonica cultivar-



group)

542
G2802

Brassica napus

gi12751304

1.20E−49

542
G2802

Petunia x hybrida

gi21105748

3.70E−09

542
G2802

Triticum

gi6732154

3.10E−08




monococcum


542
G2802

Solanum tuberosum

gi14485513

1.10E−07

542
G2802
Triticum sp.
gi4218535

3.30E−06

542
G2802

Phaseolus vulgaris

gi15148912

9.00E−06

542
G2802

Lycopersicon

gi6175246

3.50E−05




esculentum


542
G2802

Medicago

gi7716952

4.20E−05




truncatula


544
G2805

Brassica oleracea

BZ506570

4.00E−27

544
G2805

Gossypium

CA992724

6.00E−05




hirsutum


544
G2805

Glycine max

CA802497

3.00E−04

544
G2805

Lycopersicon

BE460507

0.002




esculentum


544
G2805

Eschscholzia

CD480753

0.005




californica


544
G2805

Oryza sativa

AK062955

0.005



( japonica cultivar-



group)

544
G2805

Sorghum bicolor

BE363054

0.016

544
G2805

Solanum tuberosum

BM406262

0.016

544
G2805

Gossypium

BG440924

0.035




arboreum


544
G2805

Lactuca sativa

BQ850404

0.079

544
G2805

Oryza sativa

gi21741263

0.00027



( japonica cultivar-



group)

544
G2805

Petunia x hybrida

gi21389179

0.00048

544
G2805

Oryza sativa

gi13129497

0.0027

544
G2805

Glycine max

gi22597158

0.05

544
G2805

Lycopersicon

gi6175246

0.35




esculentum


544
G2805

Phaseolus vulgaris

gi15148914

0.54

544
G2805

Medicago

gi7716952

0.61




truncatula


544
G2805

Theobroma cacao

gi15487902

0.87

544
G2805

Triticum

gi6732156

1




monococcum


544
G2805

Papaver

gi169002

1




somniferum


545
G2826

Glycine max

GLYMA-28NOV01-
753




CLUSTER166362_1

545
G2826

Glycine max

GLYMA-28NOV01-
754




CLUSTER180202_1

545
G2826

Glycine max

GLYMA-28NOV01-
755




CLUSTER726571_1

545
G2826

Glycine max

GLYMA-28NOV01-
756




CLUSTER74662_1

545
G2826

Glycine max

uC-fmflminsoy032f06b1
757

545
G2826

Oryza sativa

ORYSA-22JAN02-
758




CLUSTER173260_2

545
G2826

Oryza sativa

ORYSA-22JAN02-
759




CLUSTER200967_1

545
G2826

Oryza sativa

OSC100895.C1.p14.fg
760

545
G2826

Oryza sativa

OSC22205.C1.p1.fg
1279

545
G2826

Oryza sativa

OSC23411.C1.p1.fg
761

545
G2826

Oryza sativa

OSC2409.C1.p2.fg
762

545
G2826

Oryza sativa

OSC25680.C1.p1.fg
763

545
G2826

Zea mays

ZEAMA-08NOV01-
764




CLUSTER436044_1

545
G2826

Zea mays

ZEAMA-08NOV01-
765




CLUSTER518126_1

545
G2826

Oryza sativa

Os_S106189
1616

545
G2826

Lycopersicon

SGN-UNIGENE-54039
1959




esculentum


545
G2826

Lycopersicon

SGN-UNIGENE-54252
1960




esculentum


545
G2826

Lycopersicon

SGN-UNIGENE-
1961




esculentum

SINGLET-392715

546
G2826

Brassica oleracea

BH725134

7.00E−79

546
G2826
Oryza sativa ( indica
AAAA010048S9

1.00E−37



cultivar-group)

546
G2826

Oryza sativa

AP003214

2.00E−37

546
G2826

Lotus japonicus

BU494379

1.00E−36

546
G2826

Glycine max

BI315690

6.00E−35

546
G2826

Lycopersicon

BG135559

1.00E−33




esculentum


546
G2826

Medicago

BF004070

5.00E−32




truncatula


546
G2826

Beta vulgaris

BQ488216

4.00E−26

546
G2826

Sorghum bicolor

BE358938

1.00E−21

546
G2826

Populus

BU877646

1.00E−20



baldsamifera subsp.




trichocarpa


546
G2826

Oryza sativa

gi15528588

3.00E−35

546
G2826

Sorghum bicolor

gi18390109

8.30E−17

546
G2826

Oryza sativa

gi32482926

9.80E−07



( japonica cultivar-



group)

546
G2826

Petunia x hybrida

gi14275902

1.50E−05

546
G2826

Pisum sativum

gi7008009

0.00016

546
G2826

Zea ramosa

gi18674684

0.0002

546
G2826
Silene latifolia
gi1628463

0.00045

546
G2826

Lycopersicon

gi1345540

0.0006




esculentum


546
G2826

Nicotiana tabacum

gi14516835

0.0017

546
G2826

Rumex obtusifolius

gi20152613

0.0022

547
G2830

Glycine max

GLYMA-28NOV01-
1280




CLUSTER16384_5

548
G2830

Brassica oleracea

BH993354

9.00E−65

548
G2830

Glycine max

BM177052

5.00E−13

548
G2830

Phaseolus

CA902517

3.00E−08




coccineus


548
G2830

Lotus japonicus

AP006108

8.00E−07

548
G2830

Zea mays

BZ652013

5.9

548
G2830

Mesembtyanthemum

BG269090

7.7




crystallinum


548
G2830

Medicago

AC135797

7.7




truncatula


548
G2830

Populus

BI137362

7.7



balsamifera subsp.




trichocarpa


548
G2830
Zea mays subsp.
gi31414978

0.96




mays


548
G2830

Nicotiana tabacum

gi8099397

0.99

550
G2832

Brassica oleracea

BZ004148

4.00E−39

550
G2832

Petunia x hybrida

AB003672

8.00E−36

550
G2832

Limnanthes alba

BV007314

2.00E−20

550
G2832

Medicago

CB892199

6.00E−10




truncatula


550
G2832

Oryza sativa

AK106924

7.00E−10



( japonica cultivar-



group)

550
G2832
Oryza sativa ( indica
AAAA01002475

7.00E−10



cultivar-group)

550
G2832

Oryza sativa

AC037426

7.00E−10

550
G2832

Solanum demissum

AC136471

2.00E−08

550
G2832

Lactuca sativa

BU015249

7.00E−08

550
G2832

Zea mays

CC408365

5.00E−06

550
G2832

Petunia x hybrida

gi1786146

9.30E−50

550
G2832

Oryza sativa

gi32482980

9.10E−11



( japonica cultivar-



group)

550
G2832

Medicago sativa

gi7228329

6.40E−08

55b
G2832

Glycine max

gi1763063

7.20E−06

550
G2832

Pisum sativum

gi2129892

4.10E−05

550
G2832

Nicotiana tabacum

gi2981169

8.90E−05

550
G2832

Triticum aestivum

gi485814

0.00041

550
G2832

Oryza sativa

gi12698882

0.00046

550
G2832

Brassica rapa

gi2058504

0.00055

550
G2832

Datisca glomerata

gi4666360

0.0066

552
G2834
Oryza sativa ( indica
AAAA01001233

1.00E−126



cultivar-group)

552
G2834

Oryza sativa

AK072211

1.00E−126



( japonica cultivar-



group)

552
G2834

Oryza sativa

AP003235

1.00E−126

552
G2834

Brassica oleracea

BZ014527

1.00E−106

552
G2834

Vitis vinifera

BM436747

1.00E−105

552
G2834

Zea mays

AY106636

1.00E−101

552
G2834

Ipomoea batatas

BM878854

3.00E−95

552
G2834

Glycine max

BQ741681

3.00E−94

552
G2834

Solanum tuberosum

BE343020

3.00E−92

552
G2834

Lycopersicon

BE459539

1.00E−88




esculentum


552
G2834

Oryza sativa

gi15408708

2.40E−123

552
G2834

Oryza sativa

gi19571114

2.40E−123



( japonica cultivar-



group)

552
G2834

Lycopersicon

gi15984226

3.40E−75




esculentum


552
G2834

Glycine max

gi18376601

2.30E−29

552
G2834

Solanum tuberosum

gi563623

1.10E−20

552
G2834

Zea mays

gi3170601

1.80E−19

552
G2834

Petunia x hybrida

gi14522848

0.51

552
G2834

Nicotiana tabacum

gi4519673

0.73

552
G2834
Oryza sativa ( indica
gi28195113

0.99



cultivar-group)

552
G2834

Brassica rapa

gi7209506

1

554
G2837

Brassica oleracea

BZ451120

4.00E−55

554
G2837

Vitis vinifera

CD010326

9.00E−13

554
G2837

Glycine max

AQ842019

3.00E−09

554
G2837

Zea mays

BZ806743

3.00E−06

554
G2837
Oryza sativa ( indica
AAAA01007143

8.00E−06



cultivar-group)

554
G2837

Oryza sativa

AP003988

1.00E−05



( japonica cultivar-



group)

554
G2837

Brassica napus

CB686322

4.00E−05

554
G2837

Lotus japonicus

AP004945

9.00E−05

554
G2837

Triticum aestivum

GA708862

2.00E−04

554
G2837

Petunia x hybrida

AB000454

4.00E−04

554
G2837

Petunia x hybrida

gi1786140

2.40E−05

554
G2837

Zea diploperennis

gi1076786

0.00014

554
G2837

Pisum sativum

gi7440062

0.00027

554
G2837

Oryza sativa

gi22002132

0.0003



( japonica cultivar-



group)

554
G2837

Lycopersicon

gi1345540

0.00045




esculentum


554
G2837

Nicotiana tabacum

gi395147

0.0013

554
G2837

Brassica oleracea

gi1418351

0.0017

554
G2837

Medicago sativa

gi1279563

0.002

554
G2837

Cicer arietinum

gi21068672

0.0022

554
G2837

Zea mays

gi18568237

0.005

555
G2838

Glycine max

GLYMA-28NOV01-
753




CLUSTER166362_1

555
G2838

Glycine max

GLYMA-28NOV01-
754




CLUSTER180202_1

555
G2838

Glycine max

GLYMA-28NOV01-
755




CLUSTER726571_1

555
G2838

Glycine max

GLYMA-28NOV01-
756




CLUSTER74662_1

555
G2838

Glycine max

uC-gmflminsoy032f06b1
757

555
G2838

Oryza sativa

ORYSA-22JAN02-
759




CLUSTER200967_1

555
G2838

Oryza sativa

OSC100895.C1.p14.fg
760

555
G2838

Oryza sativa

OSC22205.C1.p1.fg
1279

555
G2838

Oryza sativa

OSC23411.C1.p1.fg
761

555
G2838

Oryza sativa

OSC2409.C1.p2.fg
762

555
G2838

Oryza sativa

OSC25680.C1.p1.fg
763

555
G2838

Zea mays

ZEAMA-08NOV01-
764




CLUSTER436044_1

555
G2838

Zea mays

ZEAMA-08NOV01-
765




CLUSTER518126_1

555
G2838

Lycopersicon

SGN-UNIGENE-54039
1959




esculentum


555
G2838

Lycopersicon

SGN-UNIGENE-54252
1960




esculentum


555
G2838

Lycopersicon

SGN-UNIGENE-
1961




esculentum

SINGLET-392715

556
G2838

Vitis vinifera

CD714231

7.00E−27

556
G2838

Gossypium

BF272143

2.00E−20




arboreum


556
G2838

Brassica oleracea

BZ444318

5.00E−20

556
G2838

Lycopersicon

BG643969

2.00E−16




esculentum


556
G2838

Zea mays

CG712091

1.00E−15

556
G2838

Sorghum bicolor

BE360413

7.00E−14

556
G2838
Oryza sativa ( indica
AAAA01009505

1.00E−12



cultivar-group)

556
G2838

Oryza sativa

AK068762

2.00E−12



( japonica cultivar-



group)

556
G2838

Medicago

BE943078

5.00E−12




truncatula


556
G2838

Glycine max

BG047435

5.00E−12

556
G2838

Sorghum bicolor

gi18390109

1.70E−14

556
G2838

Oryza sativa

gi15528588

5.90E−12

556
G2838

Oryza sativa

gi29027767

2.20E−08



( japonica cultivar-



group)

556
G2838

Petunia x hybrida

gi14275902

8.70E−05

556
G2838

Zea ramosa

gi18674684

0.02

556
G2838

Medicago sativa

gi7228329

0.024

556
G2838

Pisum sativum

gi2129892

0.06

556
G2838

Glycine max

gi1763063

0.095

556
G2838

Datisca glomerata

gi4666360

0.33

556
G2838

Nicotiana tabacum

gi2981169

0.38

557
G2839

Glycine max

GLYMA-28NOV01-
1281




CLUSTER32534_1

557
G2839

Glycine max

GLYMA-28NOV01-
1282




CLUSTER37260_1

557
G2839

Glycine max

GLYMA-28NOV01-
1283




CLUSTER379048_1

557
G2839

Oryza sativa

ORYSA-22JAN02-
1284




CLUSTER172363_1

557
G2839

Oryza sativa

ORYSA-22JAN02-
1285




CLUSTER24561_1

557
G2839

Oryza sativa

ORYSA-22JAN02-
1286




CLUSTER32611_1

557
G2839

Oryza sativa

OSC100424.C1.p49.fg
1287

557
G2839

Oryza sativa

OSC20005.C1.p5.fg
1288

557
G2839

Oryza sativa

OSC20005.C1.p7.fg
1289

557
G2839

Oryza sativa

OSC234.C1.p1.fg
1290

557
G2839

Oryza sativa

OSC5483.C1.p4.fg
1291

557
G2839

Oryza sativa

OSC5499.C1.p15.fg
1292

557
G2839

Zea mays

LIB3732-060-Q1-K6-F2
1293

557
G2839

Zea mays

ZEAMA-08NOV01-
1294




CLUSTER226176_1

557
G2839

Zea mays

ZEAMA-08NOV01-
1295




CLUSTER276871_2

557
G2839

Zea mays

ZEAMA-08NOV01-
1296




CLUSTER49287_1

557
G2839

Zea mays

ZEAMA-08NOV01-
1297




CLUSTER5148_1

557
G2839

Zea mays

ZEAMA-08NOV01-
1298




CLUSTER536916_1

557
G2839

Zea mays

ZEAMA-08NOV01-
1299




CLUSTER72147_1

557
G2839

Oryza sativa

Os_S109163
1617

557
G2839

Glycine max

Gma_S4898433
1675

557
G2839

Glycine max

Gma_S4973977
1676

557
G2839

Medicago

Mtr_S5397852
1713




truncatula


557
G2839

Hordeum vulgare

Hv_S207187
1749

557
G2839

Triticum aestivum

Ta_S111267
1914

557
G2839

Triticum aestivum

Ta_S200273
1915

557
G2839

Triticum aestivum

Ta_S296415
1916

557
G2839

Triticum aestivum

Ta_S379755
1917

557
G2839

Lycopersicon

SGN-UNIGENE-56766
2078




esculentum


558
G2839

Brassica oleracea

BZ083260

1.00E−51

558
G2839

Brassica rapa

BQ790831

6.00E−48



subsp. pekinensis

558
G2839

Brassica napus

CD842269

7.00E−48

558
G2839

Brassica rapa

L46574

7.00E−30

558
G2839

Vitis vinifera

CA818230

4.00E−25

558
G2839

Petunia x hybrida

AB006600

9.00E−25

558
G2839

Glycine max

BU577326

2.00E−23

558
G2839

Solanum tuberosum

BQ121105

4.00E−23

558
G2839

Populus tremula x

BU867080

8.00E−23




Populus tremuloides


558
G2839

Lycopersicon

AI898309

1.00E−22




esculentum


558
G2839

Petunia x hybrida

gi2346976

7.10E−27

558
G2839

Oryza sativa

gi29124132

3.90E−21



( japonica cultivar-



group)

558
G2839

Oryza sativa

gi15623820

1.30E−20

558
G2839

Glycine max

gi1763063

2.40E−16

558
G2839

Nicotiana tabacum

gi2981169

1.20E−15

558
G2839

Datisca glomerata

gi4666360

3.20E−15

558
G2839

Medicago sativa

gi7228329

2.60E−13

558
G2839

Triticum aestivum

gi485814

3.80E−13

558
G2839

Brassica rapa

gi2058506

4.60E−11

558
G2839

Pisum sativum

gi2129892

3.40E−07

560
G2846

Gossypium

CA993210

4.00E−46




hirsutum


560
G2846

Oryza sativa

AK069366

2.00E−41



( japonica cultivar-



group)

560
G2846

Brassica oleracea

BH484306

5.00E−41

560
G2846

Glycine max

BU764909

8.00E−37

560
G2846

Sorghum bicolor

CB926717

4.00E−34

560
G2846

Triticum aestivum

CA600074

1.00E−31

560
G2846

Populus

AI166770

5.00E−29



balsamifera subsp.




trichocarpa


560
G2846

Populus tremula x

BU894578

7.00E−28




Populus tremuloides


560
G2846

Gossypium

BG440718

4.00E−27




arboreum


560
G2846

Solanum tuberosum

BQ514720

2.00E−24

560
G2846

Oryza sativa

gi21741062

6.40E−42



( japonica cultivar-



group)

560
G2846

Brassica napus

gi11045087

1.60E−24

560
G2846

Oryza sativa

gi21740790

1.80E−23

560
G2846

Lycopersicon

gi5669656

2.70E−20




esculentum


560
G2846

Glycine max

gi3399777

3.50E−05

560
G2846

Cucumis melo

gi28558779

0.0011

560
G2846

Mesembryanthemum

gi4206118

0.014




crystallinum


560
G2846

Oryza rufipogon

gi1086536

0.028

560
G2846

Phyllostachys acuta

gi527661

0.039

560
G2846

Tulipa gesneriana

gi5923912

0.045

562
G2847

Glycine max

BU084566

8.00E−55

562
G2847

Populus

AI166770

3.00E−50



balsamifera subsp.




trichocarpa


562
G2847

Brassica napus

CD832456

2.00E−49

562
G2847

Solanum tuberosum

BI175830

1.00E−48

562
G2847

Ipomoea nil

BJ570931

2.00E−46

562
G2847

Oryza sativa

AK072848

4.00E−45



( japonica cultivar-



group)

562
G2847

Gossypium

BG440718

7.00E−43




arboreum


562
G2847

Populus tremula x

BU895329

5.00E−36




Populus tremuloides


562
G2847

Hordeum vulgare

BF620349

6.00E−36

562
G2847

Triticum aestivum

CA642784

5.00E−31

562
G2847

Brassica napus

gi11045087

1.40E−62

562
G2847

Oryza sativa

gi21740790

8.90E−29

562
G2847

Oryza sativa

gi32480231

8.90E−29



( japonica cultivar-



group)

562
G2847

Lycopersicon

gi5669656

1.20E−24




esculentum


562
G2847

Mesembryanthemum

gi4206118

1.20E−05




crystallinum


562
G2847

Glycine max

gi3399777

0.021

562
G2847

Phyllostachys acuta

gi527661

0.066

562
G2847

Hordeum vulgare

gi20372895

0.082



subsp. vulgare

562
G2847

Gossypioides kirkii

gi23476285

0.12

562
G2847

Pennisetum

gi527657

0.14




glaucum


564
G2850

Poncirus trifoliata

CD573726

6.00E−68

564
G2850

Lycopersicon

AI899168

1.00E−45




esculentum


564
G2850

Medicago

AL380393

8.00E−41




truncatula


564
G2850

Brassica napus

CD825720

2.00E−40

564
G2850

Gossypium

BG440718

4.00E−30




arboreum


564
G2850

Populus

AI166770

7.00E−30



balsamifera subsp.




trichocarpa


564
G2850

Populus tremula x

BU895329

4.00E−29




Populus tremuloides


564
G2850

Glycine max

BU084566

1.00E−28

564
G2850

Solanum tuberosum

BQ514720

2.00E−27

564
G2850

Gossypium

CA993210

4.00E−27




hirsutum


564
G2850

Oryza sativa

gi14488370

3.10E−26

564
G2850

Oryza sativa

gi21741062

1.60E−25



( japonica cultivar-



group)

564
G2850

Brassica napus

gi11045087

5.90E−25

564
G2850

Lycopersicon

gi5669656

1.00E−18




esculentum


564
G2850

Pennisetum

gi527657

2.40E−05




glaucum


564
G2850

Glycine max

gi3399777

8.00E−05

564
G2850

Phyllostachys acuta

gi527661

0.0061

564
G2850

Oryza australiensis

gi1086526

0.0077

564
G2850

Oryza officinalis

gi1086534

0.0077

564
G2850

Tripsacum australe

gi527663

0.01

566
G2851

Brassica napus

GD832456

7.00E−93

566
G2851

Glycine max

BU084566

5.00E−67

566
G2851

Populus

AI166770

2.00E−65



balsamifera subsp.




trichocarpa


566
G2851

Brassica oleracea

BH927065

6.00E−56

566
G2851

Solanum tuberosum

BI175830

1.00E−55

566
G2851

Gossypium

BG440718

1.00E−51




arboreum


566
G2851

Ipomoea nil

BJ570931

5.00E−51

566
G2851

Oryza sativa

AK072848

2.00E−50



( japonica cultivar-



group)

566
G2851

Populus tremula x

BU895329

3.00E−42




Populus tremuloides


566
G2851

Hordeum vulgare

BF620349

7.00E−39

566
G2851

Brassica napus

gi11045087

1.60E−162

566
G2851

Oryza sativa

gi21740790

1.10E−27

566
G2851

Oryza sativa

gi32480231

1.10E−27



( japonica cultivar-



group)

566
G2851

Lycopersicon

gi5669656

4.20E−15




esculentum


566
G2851

Phyllostachys acuta

gi527661

0.0081

566
G2851

Glycine max

gi3399777

0.012

566
G2851

Pisum sativum

gi13365610

0.018

566
G2851

Pennisetum

gi527657

0.021




glaucum


566
G2851

Tulipa gesneriana

gi5923912

0.077

566
G2851

Phaseolus vulgaris

gi1142621

0.09

567
G2854

Oryza sativa

ORYSA-22JAN02-
1300




CLUSTER907_3

567
G2854

Oryza sativa

OSC18775.C1.p1.fg
1301

567
G2854

Zea mays

ZEAMA-08NOV01-
1302




CLUSTER13712_1

567
G2854

Oryza sativa

Os_S32676
1618

567
G2854

Oryza sativa

Os_S75860
1619

567
G2854

Glycine max

Gma_S4975207
1677

567
G2854

Hordeum vulgare

Hv_S153237
1750

567
G2854

Hordeum vulgare

Hv_S63965
1751

567
G2854

Zea mays

Zm_S11522955
1823

567
G2854

Zea mays

Zm_S11525357
1824

567
G2854

Triticum aestivum

Ta_S125786
1918

567
G2854

Triticum aestivum

Ta_S152820
1919

567
G2854

Triticum aestivum

Ta_S267457
1920

567
G2854

Lycopersicon

SGN-UNIGENE-44207
2079




esculentum


567
G2854

Lycopersicon

SGN-UNIGENE-56600
2080




esculentum


567
G2854

Lycopersicon

SGN-UNIGENE-
2081




esculentum

SINGLET-17539

567
G2854

Lycopersicon

SGN-UNIGENE-
2082




esculentum

SINGLET-333119

567
G2854

Lycopersicon

SGN-UNIGENE-
2083




esculentum

SINGLET-396174

567
G2854

Lycopersicon

SGN-UNIGENE-
2084




esculentum

SINGLET-49629

568
G2854

Nicotiana

NPL292767

1.00E−143




plumbaginifolia


568
G2854

Oryza sativa

AK101593

1.00E−130



( japonica cultivar-



group)

568
G2854

Zea mays

AY107267

1.00E−128

568
G2854

Beta vulgaris

BVU313097

1.00E−110

568
G2854

Gossypium

CA993585

1.00E−109




hirsutum


568
G2854

Nicotiana tabacum

AF029351

1.00E−104

568
G2854

Brassica napus

CD832069

1.00E−104

568
G2854

Triticum aestivum

BT009299

1.00E−100

568
G2854

Medicago

CB893695

2.00E−97




truncatula


568
G2854

Prunus persica

BU045049

3.00E−92

563
G2854

Nicotiana

gi9663767

7.20E−137




plumbaginifolia


568
G2854

Oryza sativa

gi12583812

6.80E−125

568
G2854

Oryza sativa

gi32488785

6.90E−116



( japonica cultivar-



group)

568
G2854

Beta vulgaris

gi30524689

6.30E−106

568
G2854

Nicotiana tabacum

gi2708532

1.30E−98

568
G2854

Sorghum bicolor

gi22208507

6.30E−25

568
G2854

Zea mays

gi23928438

4.60E−22

568
G2854

Spinacia oleracea

gi133247

4.60E−19

568
G2854
Oryza sativa ( indica
gi4680340

4.60E−19



cultivar-group)

568
G2854

Cucumis sativus

gi7528270

5.60E−19

569
G2859

Glycine max

BE347561.1
1303

569
G2859

Lycopersicon

SGN-UNIGENE-
2085




esculentum

SINGLET-452318

570
G2859

Glycine max

BE347561

2.00E−47

570
G2859

Populus tremula x

BU811904

3.00E−47




Populus tremuloides


570
G2859

Populus tremula

BU889630

8.00E−32

570
G2859

Hordeum vulgare

CA003238

3.00E−30



subsp. vulgare

570
G2859

Sorghum

BG103016

7.00E−30




propinquum


570
G2859

Gossypium

BF273287

1.00E−29




arboreum


570
G2859

Oryza sativa

AK101063

2.00E−29



( japonica cultivar-



group)

570
G2859

Sorghum bicolor

BG048756

2.00E−29

570
G2859

Lycopersicon

AI490119

8.00E−29




esculentum


570
G2859

Medicago

CB066613

1.00E−28




truncatula


570
G2859

Oryza sativa

gi20804997

1.40E−30



( japonica cultivar-



group)

570
G2859

Tulipa gesneriana

gi5923912

4.70E−30

570
G2859

Oryza sativa

gi11862964

6.30E−29

570
G2859

Pinus taeda

gi6166283

2.40E−11

570
G2859

Brassica napus

gi27650307

2.70E−06

570
G2859

Phyllostachys acuta

gi527661

3.30E−05

570
G2859

Gossypium

gi13346182

4.20E−05




hirsutum


570
G2859

Petunia x hybrida

gi10998404

6.00E−05

570
G2859

Mesembryanthemum

gi4206118

0.00012




crystallinum


570
G2859

Glycine max

gi3399777

0.00024

571
G2865

Glycine max

GLYMA-28NOV01-
1304




CLUSTER4111_3

571
G2865

Glycine max

GLYMA-28NOV01-
1305




CLUSTER4111_4

571
G2865

Glycine max

Gma_S5127199
1678

571
G2865

Lycopersicon

SGN-UNIGENE-55990
2086




esculentum


571
G2865

Lycopersicon

SGN-UNIGENE-
2087




esculentum

SINGLET-18331

572
G2865

Brassica oleracea

BH680810

4.00E−39

572
G2865

Glycine max

BE057473

6.00E−24

572
G2865

Brassica rapa

BZ614080

2.00E−17



subsp. pekinensis

572
G2865

Medicago

AL389569

8.00E−14




truncatula


572
G2865

Lotus corniculatus

CB829442

3.00E−12



var. japonicus

572
G2865

Hedyotis

CB086514

2.00E−11




centranthoides


572
G2865

Lycopersicon

AW624871

9.00E−10




esculentum


572
G2865

Triphysaria

BM356795

1.00E−09




versicolor


572
G2865

Beta vulgaris

BQ592169

3.00E−07

572
G2865

Lotus japonicus

AG233200

7.00E−07

572
G2865

Oryza sativa

gi15528806

1.80E−08

572
G2865

Cucumis melo

gi28558779

0.00016

572
G2865

Oryza sativa

gi17385671

0.00017



( japonica cultivar-



group)

572
G2865

Lycopersicon

gi6175252

0.091




esculentum


572
G2865

Cicer arietinum

gi3641870

0.12

572
G2865

Phaseolus vulgaris

gi1142621

0.2

572
G2865

Zea mays

gi4321762

0.27

572
G2865

Prunus dulcis

gi6635842

0.85

572
G2865

Gossypium

gi1000088

0.95




barbadense


572
G2865

Brassica napus

gi27650307

0.97

574
G2866

Populus tremula x

PTR306827

2.00E−38




Populus tremuloides


574
G2866

Glycine max

BU926504

2.00E−32

574
G2866

Medicago

BF649039

9.00E−28




truncatula


574
G2866

Oryza sativa

AK103865

6.00E−25



( japonica cultivar-



group)

574
G2866

Hordeum vulgare

BG301068

5.00E−20

574
G2866

Triticum aestivum

BJ228821

9.00E−20

574
G2866

Helianthus annuus

BU018212

3.00E−18

574
G2866

Zea mays

BF727992

9.00E−18

574
G2866

Oryza sativa

AU056864

1.00E−17

574
G2866

Cycas rumphii

CB089859

7.00E−17

574
G2866

Populus tremula x

gi20269055

8.40E−40




Populus tremuloides


574
G2866

Oryza sativa

gi8096369

4.20E−25

574
G2866
Oryza sativa ( indica
gi30962267

1.40E−19



cultivar-group)

574
G2866

Cucumis sativus

gi6136832

1.80E−19

574
G2866

Triticum aestivum

gi32400272

2.30E−19

574
G2866

Zinnia elegans

gi20257219

4.70E−19

574
G2866

Vitis vinifera

gi29465672

9.10E−19

574
G2866

Vigna radiata

gi11131105

1.30E−18

574
G2866

Pisum sativum

gi1352057

2.70E−18

574
G2866

Antirrhinum majus

gi18071490

4.50E−18

576
G2869

Oryza sativa

AK070026

1.00E−120



( japonica cultivar-



group)

576
G2869

Oryza sativa

AB071300

1.00E−104

576
G2869

Mangifera indica

AY255705

7.00E−84

576
G2869

Zea mays

AY107195

1.00E−79

576
G2869
Oryza sativa ( indica
CB631221

3.00E−78



cultivar-group)

576
G2869

Medicago

BI308096

2.00E−73




truncatula


576
G2869

Triticum aestivum

BQ578824

7.00E−70

576
G2869

Pinus pinaster

BX250119

7.00E−65

576
G2869

Poncirus trifoliata

CD575895

1.00E−63

576
G2869

Solanum tuberosum

BG593647

8.00E−63

576
G2869

Oryza sativa

gi19352039

1.90E−124

576
G2869

Oryza sativa

gi20805236

1.90E−124



( japonica cultivar-



group)

576
G2869
Oryza sativa ( indica
gi26251300

5.50E−120



cultivar-group)

576
G2869

Mangifera indica

gi30027167

1.90E−116

576
G2869

Prunus persica

gi27450533

2.10E−84

576
G2869

Bruguiera

gi24371055

1.80E−53




sexangula


576
G2869

Stevia rebaudiana

gi26324158

1.80E−53

576
G2869

Pisum sativum

gi1235582

2.10E−53

576
G2869

Malus x domestica

gi1732359

2.30E−53

576
G2869

Zea mays

gi7230385

2.30E−53

578
G2884

Oryza sativa

AK100530

1.00E−46



( japonica cultivar-



group)

578
G2884

Zea mays

AB062095

6.00E−46

578
G2884
Oryza sativa ( indica
CB630542

6.00E−45



cultivar-group)

578
G2884

Solanum tuberosum

BM407041

4.00E−38

578
G2884

Medicago

CB891281

1.00E−32




truncatula


578
G2884

Brassica napus

CD825309

5.00E−32

578
G2884

Vitis vinifera

CD800109

8.00E−32

578
G2884

Sorghum bicolor

CD424269

7.00E−31

578
G2884

Stevia rebaudiana

BG523436

7.00E−30

578
G2884

Lactuca sativa

BQ858556

2.00E−29

578
G2884

Zea mays

gi14189890

2.50E−47

578
G2884

Oryza sativa

gi24308616

3.40E−44



( japonica cultivar-



group)

578
G2884

Oryza glabberima

gi31338862

3.90E−35

578
G2884
Oryza sativa ( indica
gi31338860

2.10E−34



cultivar-group)

578
G2884

Oryza sativa

gi15289981

2.70E−13

578
G2884

Nicotiana tabacum

gi4519671

3.70E−06

578
G2884

Chlamydomonas

gi5916207

1.50E−05




reinhardtii


578
G2884

Mesernbryanthemum

gi6942190

0.00012




crystallinum


578
G2884

Solanum

gi32470629

0.00013




bulbocastanum


578
G2884
Dianthus
gi13173408

0.026




caryophyllus


579
G2885

Oryza sativa

ORYSA-22JAN02-
1306




CLUSTER270810_1

579
G2885

Lycopersicon

SGN-UNIGENE-
2088




esculentum

SINGLET-66716

580
G2885

Oryza sativa

AK100530

5.00E−81



( japonica cultivar-



group)

580
G2885

Zea mays

AB062095

4.00E−80

580
G2885
Oryza sativa ( indica
CB630542

8.00E−72



cultivar-group)

580
G2885

Solanum tuberosum

BM407041

3.00E−54

580
G2885

Medicago

CB891281

1.00E−49




truncatula


580
G2885

Vitis vinifera

CD800109

7.00E−46

580
G2885

Stevia rebaudiana

B0523436

2.00E−45

580
G2885

Lactuca sativa

BQ858556

2.00E−45

580
G2885

Glycine max

AW596288

2.00E−42

580
G2885

Sorghum bicolor

CD424269

2.00E−42

580
G2885

Zea mays

gi14189890

6.70E−79

580
G2885

Oryza sativa

gi24308616

9.40E−72



( japonica cultivar-



group)

580
G2885
Oryza sativa ( indica
gi31338860

4.20E−40



cultivar-group)

580
G2885

Oryza glabberima

gi31338862

9.30E−40

580
G2885

Oryza sativa

gi15289981

1.80E−18

580
G2885

Nicotiana tabacum

gi4519671

1.60E−09

580
G2885

Solanum

gi32470629

4.10E−09




bulbocastanum


580
G2885

Chlamydomonas

gi5916207

4.90E−08




reinhardtii


580
G2885

Mesembryanthemum

gi6942190

1.20E−07




crystallinum


580
G2885

Brassica napus

gi10041875

0.00086

582
G2887

Brassica napus

CD828428

9.00E−90

582
G2887

Medicago

AF254124

3.00E−73




truncatula


582
G2887

Petunia x hybrida

AF509865

2.00E−70

582
G2887

Prunus persica

BU044475

4.00E−70

582
G2887

Oryza sativa

AK068153

8.00E−67



( japonica cultivar-



group)

582
G2887

Hordeum vulgare

BJ481205

1.00E−63



subsp. spontaneum

582
G2887

Oryza sativa

AX654724

2.00E−61

582
G2887

Sorghum

BG159075

4.00E−61




propinquum


582
G2887

Brassica oleracea

BH986593

5.00E−61

582
G2887

Solanum tuberosum

BQ118148

6.00E−61

582
G2887

Medicago

gi7716952

2.20E−71




truncatula


582
G2887

Petunia x hybrida

gi21105732

3.30E−70

582
G2887

Oryza sativa

gi27452910

6.50E−49



( japonica cultivar-



group)

582
G2887

Oryza sativa

gi6730946

3.90E−42

582
G2887

Glycine max

gi22597158

1.80E−39

582
G2887

Phaseolus vulgaris

gi15148914

7.80E−37

582
G2887

Brassica napus

gi31322572

7.00E−36

582
G2887

Triticum sp.

gi4218537

1.90E−35

582
G2887

Triticum

gi6732160

1.90E−35




monococcum


582
G2887

Solanum tuberosum

gi14485513

2.40E−35

584
G2888

Oryza sativa

AK106796

8.00E−76



( japonica cultivar-



group)

584
G2888

Brassica oleracea

BH707475

1.00E−72

584
G2888

Zea mays

BZ821684

3.00E−69

584
G2888
Oryza sativa ( indica
AAAA01002232

8.00E−69



cultivar-group)

584
G2888

Physcomitrella

BJ192201

1.00E−67



patens subsp. patens

584
G2888

Glycine max

BI972592

4.00E−67

584
G2888

Medicago

BI265111

1.00E−64




truncatula


584
G2888

Triticum aestivum

BQ806659

3.00E−56

584
G2888

Capsicum annuum

BM063853

6.00E−56

584
G2888

Phaseolus

CA902521

6.00E−56




coccineus


584
G2888

Oryza sativa

gi27357980

2.90E−77



( japonica cultivar-



group)

584
G2888

Solanum tuberosum

gi563623

7.60E−55

584
G2888

Zea mays

gi3170601

3.20E−53

584
G2888

Lycopersicon

gi9858780

1.50E−51




esculentum


584
G2888

Oryza sativa

gi10934090

1.00E−50

584
G2888

Glycine max

gi18376601

3.30E−11

584
G2888

Cucurbita maxima

gi17221648

0.032

584
G2888

Chlorella vulgaris

gi2224373

0.11

584
G2888

Helianthus annuus

gi349267

0.16

584
G2888

Petunia x hybrida

gi14275902

0.17

586
G2898

Medicago

AJ501279

2.00E−41




truncatula


586
G2898

Glycine max

BG651880

2.00E−41

586
G2898

Solanum tuberosum

BQ516260

3.00E−35

586
G2898

Populus tremula

BU816897

8.00E−32

586
G2898

Zinnia elegans

AU292820

4.00E−30

586
G2898

Oryza sativa

AK064663

7.00E−30



( japonica cultivar-



group)

586
G2898

Zea mays

CD999897

5.00E−29

586
G2898

Triticum aestivum

BM135160

2.00E−28

586
G2898

Gossypium

BG446904

6.00E−21




arboreum


586
G2898

Nuphar advena

CD475578

1.00E−19

586
G2898

Vicia faba

gi541981

1.60E−20

586
G2898

Oryza sativa

gi20161572

3.90E−19



( japonica cultivar-



group)

586
G2898

Ipomoea nil

gi1052956

6.30E−19

586
G2898

Solanum tuberosum

gi2894109

1.00E−18

586
G2898

Pisum sativum

gi436424

1.00E−18

586
G2898

Nicotiana tabacum

gi2196548

2.80E−16

586
G2898

Glycine max

gi123379

5.90E−16

586
G2898

Canavalia gladiata

gi1813329

7.50E−16

586
G2898

Narcissus

gi18419623

2.50E−15




pseudonarcissus


586
G2898
Oryza sativa ( indica
gi23345287

2.50E−15



cultivar-group)

587
G2907

Glycine max

GLYMA-28NOV01-
1307




CLUSTER30744_2

587
G2907

Oryza sativa

OSC100568.C1.p1.fg
1308

587
G2907

Oryza sativa

OSC9760.C1.p1.fg
1309

587
G2907

Zea mays

ZEAMA-08NOV01-
1310




CLUSTER298_131

587
G2907

Zea mays

ZEAMA-08NOV01-
1311




CLUSTER298_90

587
G2907

Oryza sativa

Os_S108142
1620

587
G2907

Oryza sativa

Os_S31898
1621

587
G2907

Oryza sativa

Os_S33014
1622

587
G2907

Glycine max

Gma_S5116414
1679

587
G2907

Hordeum vulgare

Hv_S52718
1752

587
G2907

Zea mays

Zm_S11441767
1825

587
G2907

Zea mays

Zm_S11491750
1826

587
G2907

Lycopersicon

Les_S5295643
1934




esculentum


587
G2907

Lycopersicon

SGN-UNIGENE-
2089




esculentum

SINGLET-472560

588
G2907

Oryza sativa

AK105116

1.0e−999



( japonica cultivar-



group)

588
G2907

Brassica napus

AF491304

1.0e−999

588
G2907

Nicotiana tabacum

AF253511

1.0e−999

588
G2907

Lycopersicon

AF096260

1.00E−174




esculentum


588
G2907
Oryza sativa ( indica
AAAA01006749

1.00E−121



cultivar-group)

588
G2907

Brassica oleracea

BZ507433

1.00E−113

588
G2907

Oryza sativa

AC079935

1.00E−106

588
G2907

Hordeum vulgare

BM817419

3.00E−70



subsp. vulgare

588
G2907

Zea mays

BZ998506

4.00E−68

588
G2907

Capsicum annuum

BM063122

5.00E−63

588
G2907

Oryza sativa

gi19920098
2.20E−229



( japonica cultivar-



group)

588
G2907

Brassica napus

gi20127124

3.60E−208

588
G2907

Nicotiana tabacum

gi11612392

2.20E−198

588
G2907

Lycopersicon

gi5669650

4.70E−117




esculentum


588
G2907

Petroselinum

gi1084392

6.00E−43




crispum


588
G2907

Triticum aestivum

gi32400790

4.00E−07

588
G2907

Pennisetum ciliare

gi549986

9.00E−06

588
G2907

Glycine max

gi17645766

8.40E−05

588
G2907

Oryza sativa

gi19070767

0.00016

588
G2907

Chlamydomonas

gi28207761

0.0013




reinhardtii


589
G2913

Glycine max

GLYMA-28NOV01-
1312




CLUSTER38751_1

589
G02913

Zea mays

ZEAMA-08NOV01-
1313




CLUSTER93389_1

589
G2913

Oryza sativa

Os_S107744
1623

590
G2913

Oryza sativa

AK065847

2.00E−57



( japonica cultivar-



group)

590
G2913

Glycine max

CD487091

1.00E−52

590
G2913

Solanum tuberosum

BM404700

4.00E−45

590
G2913

Medicago

BF004903

9.00E−45




truncatula


590
G2913

Citrus sinensis

BQ623105

1.00E−34

590
G2913

Lycopersicon

BF051118

1.00E−34




esculentum


590
G2913
Oryza sativa ( indica
CB634137

1.00E−33



cultivar-group)

590
G2913

Brassica oleracea

BZ021766

4.00E−33

590
G2913

Amborella

CD483238

3.00E−31




trichopoda


590
G2913

Populus tremula

BU889297

1.00E−30

590
G2913

Oryza sativa

gi32490476

2.40E−74



( Japonica cultivar-



group)

590
G2913

Vicia faba

gi541981

1.30E−08

590
G2913

Solanum tuberosum

gi2894109

1.70E−07

590
G2913

Nicotiana tabacum

gi2196548

7.20E−06

590
G2913

Zea mays

gi8920409

4.70E−05

590
G2913
Oryza sativa ( indica
gi21314337

7.90E−05



cultivar-group)

590
G2913

Daucus carota

gi3551257

0.0002

590
G2913

Canavalia gladiata

gi1813329

0.0011

590
G2913

Narcissus

gi18419623

0.0018




pseudonarcissus


590
G2913

Ipomoea nil

gi1085860

0.049

591
G2930

Zea mays

Zm_S11448159
1827

591
G2930

Lycopersicon

Les_S5268274
1935




esculentum


592
G2930

Triticum aestivum

CD872621

1.00E−12

592
G2930

Lycopersicon

BI203387

4.00E−09




esculentum


592
G2930

Populus tremula x

BU884102

8.00E−09




Populus tremuloides


592
G2930

Sorghum bicolor

CD428713

2.00E−08

592
G2930
Oryza sativa ( indica
CB624355

7.00E−08



cultivar-group)

592
G2930

Zinnia elegans

AU288915

1.00E−07

592
G2930

Oryza sativa

CB660906

2.00E−07



( japonica cultivar-



group)

592
G2930

Oryza sativa

AP003683

6.00E−05

592
G2930

Glycine max

BQ611037

6.00E−05

592
G2930

Medicago

BG456206

2.00E−04




truncatula


592
G2930

Oryza sativa

gi15528806

1.40E−09

592
G2930

Oryza sativa

gi29788848

0.016



( japonica cultivar-



group)

592
G2930

Pennisetum

gi527655

0.56




glaucum


592
G2930

Phyllostachys acuta

gi527661

0.61

592
G2930

Zea mays

gi100921

0.7

592
G2930

Sorghum bicolor

gi527667

0.8

592
G2930

Tripsacum australe

gi527663

0.89

592
G2930

Glycine max

gi3399777

0.9

592
G2930

Phaseolus vulgaris

gi1142619

0.97

592
G2930

Catharanthus

gi3954807

1




roseus


593
G2933

Glycine max

GLYMA-28NOV01-
1314




CLUSTER243321_1

593
G2933

Oryza sativa

OSC7496.C1.p10.fg
1315

593
G2933

Zea mays

ZEAMA-08NOV01-
1316




CLUSTER88899_1

593
G2933

Oryza sativa

Os_S39118
1624

593
G2933

Zea mays

Zm_S11445525
1828

593
G2933

Lycopersicon

SGN-UNIGENE-53603
2090




esculentum


594
G2933

Brassica oleracea

BH587081

6.00E−59

594
G2933

Populus tremula x

BU884102

8.00E−37




Populus tremuloides


594
G2933

Lycopersicon

BI205905

6.00E−29




esculentum


594
G2933

Glycine max

BQ611037

4.00E−28

594
G2933

Triticum aestivum

CD872523

4.00E−24

594
G2933

Lupinus albus

CA410291

4.00E−23

594
G2933

Oryza sativa

CB660906

5.00E−23



( japonica cultivar-



group)

594
G2933
Oryza sativa ( indica
CB624355

1.00E−22



cultivar-group)

594
G2933

Medicago

AC125478

6.00E−21




truncatula


594
G2933

Zinnia elegans

AU288915

9.00E−20

594
G2933

Oryza sativa

gi15528806

3.90E−26

594
G2933

Pennisetum

gi527657

8.60E−07



glaucum

594
G2933

Phyllostachys acuta

gi527661

4.10E−05

594
G2933

Sorghum bicolor

gi527667

5.60E−05

594
G2933

Tripsacum australe

gi527663

0.00024

594
G2933

Mesembryanthemum

gi4206118

0.00048




crystallinum


594
G2933

Oryza sativa

gi20521292

0.0012



( japonica cultivar-



group)

594
G2933

Zea mays

gi18542170

0.0014

594
G2933

Oryza australiensis

gi1086526

0.0031

594
G2933

Oryza rufipogon

gi1086538

0.0055

596
G2934

Brassica oleracea

BH680810

3.00E−50

596
G2934

Glycine max

BE057473

1.00E−28

596
G2934

Brassica rapa

BZ614080

9.00E−24



subsp. pekinensis

596
G2934

Medicago

AL389569

4.00E−14




truncatula


596
G2934

Lotus corniculatus

CB829442

5.00E−14



var. japonicus

596
G2934

Hedyotis

CB086514

2.00E−13




centranthoides


596
G2934

Lycopersicon

AW624871

7.00E−13




esculentum


596
G2934

Triphysaria

BM356795

6.00E−12



versicolor

596
G2934

Beta vulgaris

BQ592169

2.00E−10

596
G2934

Triticum aestivum

CD452898

2.00E−08

596
G2934

Oryza sativa

gi15528806

8.90E−13

596
G2934

Oryza sativa

gi17385671

1.00E−05



( japonica cultivar-



group)

596
G2934

Brassica napus

gi27650307

0.0022

596
G2934

Phaseolus vulgaris

gi1142619

0.0024

596
G2934

Cucumis melo

gi28558779

0.0029

596
G2934

Zea mays

gi100874

0.014

596
G2934

Cicer arietinum

gi3641870

0.18

596
G2934

Brassica oleracea

gi12049596

0.26

596
G2934

Pennisetum

gi527655

0.33




glaucum


596
G2934

Phyllostachys acuta

gi527661

0.37

598
G2958

Gossypium

GH1458442

3.00E−39




hirsutum


598
G2958

Prunus persica

BU041850

1.00E−34

598
G2958

Capsicum annuum

BM063701

3.00E−34

598
G2958

Solanum tuberosum

BG890076

4.00E−34

598
G2958

Vitis vinifera

CB921417

1.00E−33

598
G2958

Poncirus trifoliata

CD576407

1.00E−33

598
G2958

Brassica oleracea

BH695204

2.00E−32

598
G2958

Lactuca sativa

BQ991083

2.00E−32

598
G2958

Medicago

BF647616

4.00E−29




truncatula


598
G2958

Cucumis sativus

AB029148

9.00E−29

598
G2958

Gossypium

gi22531416

5.20E−40




hirsutum


598
G2958
Oryza sativa ( indica
gi30962267

1.50E−35



cultivar-group)

598
G2958

Oryza sativa

gi17154533

7.70E−35

598
G2958

Populus tremula x

gi20269059

8.40E−34




Populus tremuloides


598
G2958

Pinus taeda

gi32396301

2.50E−32

598
G2958

Triticum aestivum

gi32400272

3.90E−32

598
G2958

Cucumis sativus

gi6136834

4.10E−32

598
G2958

Vigna radiata

gi11131101

1.10E−30

598
G2958

Nicotiana tabacum

gi4887012

6.00E−30

598
G2958

Solanum tuberosum

gi25989504

1.20E−29

600
G2964

Brassica oleracea

BH549078

2.00E−76

600
G2964

Brassica napus

CD822240

4.00E−67

600
G2964

Zea mays

AY104443

4.00E−60

600
G2964

Solanum tuberosum

BQ505464

6.00E−54

600
G2964

Lotus corniculatus

AP006377

2.00E−50



var. japonicus

600
G2964

Hordeum vulgare

BI957620

2.00E−49

600
G2964

Gossypium

BE053739

2.00E−49




arboreum


600
G2964

Glycine max

AW348431

3.00E−48

600
G2964
Oryza sativa ( indica
CB635890

1.00E−47



cultivar-group)

600
G2964

Oryza sativa

CB660704

1.00E−47



( japonica cultivar-



group)

600
G2964

Oryza sativa

gi5441893

3.20E−47

600
G2964

Oryza sativa

gi32488659

8.60E−47



( japonica cultivar-



group)

600
G2964

Pisum sativum

gi14018368

5.30E−09

600
G2964

Pinus pinaster

gi18129298

9.80E−07

600
G2964

Brassica napus

gi1171040

0.012

600
G2964

Brassica oleracea

gi18266051

0.012

600
G2964

Zea mays

gi459269

0.28

600
G2964

Mimulus guttatus

gi127382

0.79

600
G2964

Glycine max

gi532703

0.81

600
G2964

Hordeum vulgare

gi4456620

0.92

602
G2967

Brassica oleracea

BZ064831

2.00E−51

602
G2967

Petunia x hybrida

AB006606

8.00E−06

602
G2967

Medicago

BG582425

1.00E−05




truncatula


602
G2967

Brassica napus

CB686322

2.00E−04

602
G2967

Vitis vinifera

CB008696

0.004

602
G2967

Vitis aestivalis

CB074763

0.004

602
G2967

Glycine max

BF324612

0.005

602
G2967

Oryza sativa

AY219847

0.006



( japonica cultivar-



group)

602
G2967

Lactuca sativa

BQ868010

0.006

602
G2967

Oryza sativa

CNS08CA5

0.006

602
G2967

Petunia x hybrida

gi2346988

4.40E−10

602
G2967

Medicago sativa

gi7228329

0.00039

602
G2967

Pisum sativum

gi2129892

0.00076

602
G2967

Oryza sativa

gi28849865

0.001



( japonica cultivar-



group)

602
G2967

Brassica rapa

gi2058504

0.0015

602
G2967

Triticum aestivum

gi485814

0.0052

602
G2967

Oryza sativa

gi12698882

0.0094

602
G2967

Datisca glomerata

gi4666360

0.01

602
G2967

Glycine max

gi1763063

0.014

602
G2967

Nicotiana tabacum

gi2981169

0.018

603
G2969

Oryza sativa

OSC100047.C1.p3.fg
1317

603
G2969

Oryza sativa

Os_S108460
1625

603
G2969

Glycine max

Gma_S4895054
1680

603
G2969

Glycine max

Gma_S4932729
1681

603
G2969

Glycine max

Gma_S4971594
1682

603
G2969

Lycopersicon

Les_S5265247
1936




esculentum


603
G2969

Lycopersicon

SGN-UNIGENE-57728
2091




esculentum


604
G2969

Brassica oleracea

BH481194

1.00E−107

604
G2969

Medicago

BI311225

9.00E−76




truncatula


604
G2969

Lactuca sativa

BQ874458

1.00E−70

604
G2969

Prunus persica

BU040439

3.00E−69

604
G2969

Helianthus annuus

BQ967662

2.00E−65

604
G2969

Populus tremuloides

CA932621

7.00E−65

604
G2969
Oryza sativa ( indica
AAAA01007031

1.00E−62



cultivar-group)

604
G2969

Oryza sativa

AC135205

1.00E−62



( japonica cultivar-



group)

604
G2969

Solanum tuberosum

BQ115018

7.00E−62

604
G2969

Glycine max

BE822664

1.00E−61

604
G2969

Oryza sativa

gi29893575

2.90E−62



( japonica cultivar-



group)

604
G2969

Oryza sativa

gi5777616

3.70E−46

604
G2969

Glycine max

gi28542706

0.083

604
G2969

Chlorella vulgaris

gi2224427

0.13

604
G2969

Chloroplast

gi7515285

0.13




Chlorella vulgaris


604
G2969

Petunia x hybrida

gi100396

0.88

604
G2969

Nicotiana tabacum

gi8099397

0.94

604
G2969

Pisum sativum

gi3088648

1

606
G2972

Brassica oleracea

BZ070725

1.00E−13

606
G2972

Oryza sativa

OSJN00040

2.00E−04

606
G2972

Oryza sativa

AK108645

2.00E−04



( japonica cultivar-



group)

606
G2972
Oryza sativa ( indica
AAAA01000197

6.00E−04



cultivar-group)

606
G2972

Brassica napus

CD844215

0.002

606
G2972

Petunia x hybrida

AB000454

0.005

606
G2972

Populus tremula x

BU813541

0.008




Populus tremuloides


606
G2972

Citrus sinensis

CB293113

0.025

606
G2972

Lotus japonicus

AP004523

0.025

606
G2972

Lotus corniculatus

CB828590

0.025



var. japonicus

606
G2972

Glycine max

gi1763063

6.00E−08

606
G2972

Oryza sativa

gi12698882

2.50E−06

606
G2972

Petunia x hybrida

gi439487

4.70E−06

606
G2972

Nicotiana tabacum

gi2981169

4.90E−06

606
G2972

Datisca glomerata

gi4666360

1.20E−05

606
G2972

Medicago sativa

gi7228329

1.30E−05

606
G2972

Triticum aestivum

gi485814

3.50E−05

606
G2972

Oryza sativa

gi29124140

9.50E−05



( japonica cultivar-



group)

606
G2972

Brassica rapa

gi2058506

0.00024

606
G2972

Pisum sativum

gi2129892

0.027

607
G2979

Lycopersicon

SGN-UNIGENE-49425
2092




esculentum


608
G2979

Zea mays

AY107996

2.00E−68

608
G2979

Thellungiella

BI698460

1.00E−60




salsuginea


608
G2979

Vitis vinifera

CB920900

4.00E−45

608
G2979

Helianthus annuus

CD853183

2.00E−41

608
G2979

Medicago

BG450549

3.00E−39




truncatula


608
G2979

Glycine max

BM524804

8.00E−38

608
G2979

Lycopersicon

BI924306

8.00E−37




esculentum


608
G2979

Solanum tuberosum

BE920312

7.00E−32

608
G2979

Eschscholzia

CD478692

9.00E−32




californica


608
G2979

Sorghum bicolor

BG273641

5.00E−28

608
G2979

Nicotiana tabacum

gi6328415

4.40E−10

608
G2979

Physcomitrella

gi26190147

1.00E−09




patens


608
G2979

Triticum

gi13619655

3.90E−09




monococcum


608
G2979
Triticum sp.
gi5763821

3.90E−09

608
G2979

Daucus carota

gi8977833

5.80E−09

608
G2979

Oryza sativa

gi12225043

9.90E−09

608
G2979

Chenopodium

gi11558192

3.00E−08




rubrum


608
G2979

Populus alba

gi27802536

3.10E−08

608
G2979

Oryza sativa

gi32479738

1.10E−07



( japonica cultivar-



group)

608
G2979

Thlaspi

gi22086272

2.90E−07




caerulescens


609
G2981

Glycine max

GLYMA-28NOV01-
1318




CLUSTER28852_1

609
G2981

Glycine max

GLYMA-28NOV01-
1319




CLUSTER28852_2

609
G2981

Glycine max

GLYMA-28NOV01-
1320



CLUSTER28852_4

609
G2981

Glycine max

GLYMA-28NOV01-
1321




CLUSTER28852_5

609
G2981

Glycine max

GLYMA-28NOV01-
1322




CLUSTER28852_6

609
G2981

Glycine max

GLYMA-28NOV01-
1323




CLUSTER28852_8

609
G2981

Glycine max

GLYMA-28NOV01-
1324




CLUSTER28852_9

609
G2981

Glycine max

LIB3242-344-Q1-J1-G7
1325

609
G2981

Glycine max

LIB4392-029-R1-K1-C8
1326

609
G2981

Oryza sativa

ORYSA-22JAN02-
1327




CLUSTER89637_1

609
G2981

Oryza sativa

Os_S104685
1626

609
G2981

Glycine max

Gma_S4882455
1683

609
G2981

Zea mays

Zm_S11334447
1829

609
G2981

Zea mays

Zm_S11524241
1830

609
G2981

Lycopersicon

SGN-UNIGENE-50978
2093




esculentum


610
G2981

Populus tremula x

AY307373

1.00E−123




Populus tremuloides


610
G2981

Oryza sativa

AY224589

1.00E−106



( japonica cultivar-



group)

610
G2981

Zea mays

AY108383

1.00E−105

610
G2981

Poncirus trifoliata

CD573622

1.00E−96

610
G2981

Glycine max

BU579005

8.00E−85

610
G2981

Solanum tuberosum

BM406319

6.00E−79

610
G2981

Lycopersicon

BG134590

2.00E−76




esculentum


610
G2981

Pinus taeda

BG040894

4.00E−74

610
G2981

Marchantia

C96290

2.00E−71




polymorpha


610
G2981

Lactuca sativa

BU012590

4.00E−66

610
G2981

Populus tremula x

gi32187097

8.20E−119




Populus tremuloides


610
G2981

Oryza sativa

gi29371983

2.80E−101



( japonica cultivar-



group)

610
G2981
Triticum sp.
gi11877791

4.10E−47

610
G2981

Triticum

gi13619653

4.10E−47




monococcum


610
G2981

Populus alba

gi27802536

0.0064

610
G2981

Gnetum gnemon

gi5019435

0.037

610
G2981

Nicotiana tabacum

gi6328415

0.069

610
G2981

Oryza sativa

gi12225043

0.071

610
G2981

Physcomitrella

gi26190147

0.099




patens


610
G2981

Chenopodium

gi11558192

0.15




rubrum


611
G2982

Glycine max

GLYMA-28NOV01-
1318




CLUSTER28852_1

611
G2982

Glycine max

GLYMA-28NOV01-
1319




CLUSTER28852_2

611
G2982

Glycine max

GLYMA-28NOV01-
1320




CLUSTER28852_4

611
G2982

Glycine max

GLYMA-28NOV01-
1321




CLUSTER28852_5

611
G2982

Glycine max

GLYMA-28NOV01-
1322




CLUSTER28852_6

611
G2982

Glycine max

GLYMA-28NOV01-
1323




CLUSTER28852_8

611
G2982

Glycine max

LIB3242-344-Q1-J1-G7
1325

611
G2982

Glycine max

LIB4392-029-R1-K1-C8
1326

611
G2982

Oryza sativa

ORYSA-22JAN02-
1327




CLUSTER89637_1

611
G2982

Lycopersicon

SGN-UNIGENE-50978
2093




esculentum


612
G2982

Brassica napus

CD813391

1.00E−79

612
G2982

Populus tremula x

AY307373

2.00E−59




Populus tremuloides


612
G2982

Zea mays

AY108383

6.00E−57

612
G2982

Oryza sativa

AY224551

2.00E−54



( japonica cultivar-



group)

612
G2982

Glycine max

BU579005

8.00E−52

612
G2982

Pinus taeda

BG040894

3.00E−50

612
G2982

Solanum tuberosum

BM406319

2.00E−47

612
G2982

Marchantia

C96290

3.00E−47




polymorpha


612
G2982

Lycopersicon

BM412584

1.00E−42




esculentum


612
G2982
Triticum sp.
TSP271917

9.00E−40

612
G2982

Populus tremula x

gi32187097

1.20E−58




Populus tremuloides


612
G2982

Oryza sativa

gi29367654

6.80E−54



( japonica cultivar-



group)

612
G2982
Triticum sp.
gi11877791

2.00E−40

612
G2982

Triticum

gi13619653

2.00E−40




monococcum


612
G2982

Daucus carota

gi8977833

0.0044

612
G2982

Nicotiana tabacum

gi6328415

0.057

612
G2982

Physcomitrella

gi26190147

0.17




patens


612
G2982

Thlaspi

gi22086272

0.21




caerulescens


612
G2982

Oryza sativa

gi12225043

0.24

612
G2982

Chenopodium

gi11558192

0.25




rubrum


613
G2983

Glycine max

GLYMA-28NOV01-
1328




CLUSTER73435_1

613
G2983

Glycine max

GLYMA-28NOV01-
1329




CLUSTER73435_2

613
G2983

Glycine max

GLYMA-28NOV01-
1330




CLUSTER73435_3

613
G2983

Oryza sativa

OSC101922.C1.p4.fg
1331

613
G2983

Oryza sativa

OSC102287.C1.p18.fg
1332

613
G2983

Oryza sativa

OSC32392.C1.p1.fg
1333

613
G2983

Oryza sativa

Os_S83060
1627

613
G2983

Glycine max

Gma_S4865673
1684

613
G2983

Medicago

Mtr_S5361396
1714




truncatula


613
G2983

Lycopersicon

SGN-UNIGENE-49230
2094




esculentum


614
G2983

Glycine max

AX105305

3.00E−57

614
G2983

Medicago

BG588588

2.00E−55




truncatula


614
G2983

Populus tremula x

BU828082

8.00E−51




Populus tremuloides


614
G2983

Brassica oleracea

BH250068

9.00E−50

614
G2983

Lycopersicon

BG134747

2.00E−48




esculentum


614
G2983

Beta vulgaris

BQ593610

3.00E−47

614
G2983

Lupinus albus

CA410814

3.00E−44

614
G2983

Populus

GA826202

9.00E−36



balsamifera subsp.




trichocarpa x





Populus deltoides


614
G2983

Zea mays

CC347703

1.00E−27

614
G2983

Oryza sativa

OSJN00105

3.00E−27

614
G2983

Oryza sativa

gi10241438

8.10E−35

614
G2983

Oryza sativa

gi21740884

4.10E−31



( japonica cultivar-



group)

614
G2983

Petunia x hybrida

gi22087128

3.60E−22

614
G2983

Lycopersicon

gi28070968

5.80E−22




esculentum


614
G2983

Populus tremula x

gi3955021

4.50E−10




Populus trernuloides


614
G2983

Narcissus

gi18419580

3.90E−05




pseudonarcissus


614
G2983

Ceratopteris

gi3868829

0.025




richardii


614
G2983

Physcomitrella

gi7415618

0.079




patens


614
G2983

Helianthus annuus

gi349379

0.15

614
G2983

Zinnia elegans

gi24417147

0.3

615
G2990

Oryza sativa

OSC4898.C1.p6.fg
1334

615
G2990

Zea mays

LIB3279-221-Q6-K6-B2
1335

615
G2990

Zea mays

ZEAMA-08NOV01-
1336




CLUSTER42733_1

615
G2990

Oryza sativa

Os_S56831
1628

615
G2990

Glycine max

Gma_S4897246
1685

615
G2990

Medicago

Mtr_S5341529
1715




truncatula


615
G2990

Triticum aestivum

Ta_S171947
1921

615
G2990

Lycopersicon

SGN-UNIGENE-49426
2095




esculentum


615
G2990

Lycopersicon

SGN-UNIGENE-52525
2096




esculentum


616
G2990

Brassica oleracea

BH738007

1.00E−100

616
G2990

Medicago

AC139600

3.00E−84




truncatula


616
G2990

Flaveria bidentis

FBI18580

8.00E−81

616
G2990

Glycine max

BF069575

4.00E−59

616
G2990

Solanum tuberosum

BE471989

7.00E−56

616
G2990

Flaveria trinervia

FTR18577

3.00E−51

616
G2990

Populus

AI166342

5.00E−45



balsamifera subsp.




trichocarpa


616
G2990

Vitis vinifera

CB970621

7.00E−45

616
G2990

Oryza sativa

AP005152

2.00E−43



( japonica cultivar-



group)

616
G2990

Zea mays

GC335993

3.00E−42

616
G2990

Flaveria bidentis

gi13277220

1.10E−76

616
G2990

Oryza sativa

gi32480091

2.10E−38



( japonica cultivar-



group)

616
G2990

Flaveria trinervia

gi13277216

1.60E−29

616
G2990

Oryza sativa

gi5091602

3.00E−28

616
G2990

Lactuca sativa

gi29119890

9.00E−20

616
G2990

Bromheadia

gi2108256

4.30E−06




finlaysoniana


616
G2990

Lycopersicon

gi100214

1.20E−05




esculentum


616
G2990

Daucus carota

gi224556

1.70E−05

616
G2990

Nicotiana alata

gi1247388

1.90E−05

616
G2990

Gossypium

gi451544

3.80E−05




barbadense


617
G2992

Oryza sativa

Os_S94181
1629

617
G2992

Zea mays

Zm_S11399262
1831

618
G2992

Brassica oleracea

BZ087784

3.00E−94

618
G2992

Brassica napus

CD817420

4.00E−65

618
G2992

Medicago

AC139600

6.00E−51




truncatula


618
G2992

Flaveria bidentis

FBI18580

6.00E−48

618
G2992

Glycine max

BF069575

1.00E−47

618
G2992

Oryza sativa

AP005152

9.00E−44



( japonica cultivar-



group)

618
G2992

Vitis vinifera

CB970621

3.00E−43

618
G2992

Flaveria trinervia

FTR18577

2.00E−42

618
G2992

Zea mays

CC335993

4.00E−42

618
G2992

Helianthus annuus

BU022145

8.00E−42

618
G2992

Flaveria bidentis

gi13277220

9.50E−52

618
G2992

Flaveria trinervia

gi13277216

4.40E−43

618
G2992

Oryza sativa

gi5091602

4.40E−40

618
G2992

Oryza sativa

gi32480091

4.90E−28



( japonica cultivar-



group)

618
G2992

Lactuca sativa

gi29119890

3.50E−18

618
G2992

Sorghum bicolor

gi671656

0.12

618
G2992

Pisum sativum

gi15021756

0.15

618
G2992

Lycopersicon

gi1345538

0.24




esculentum


618
G2992

Nicotiana tabacum

gi119714

0.67

618
G2992

Pinus taeda

gi1076237

0.67

620
G2993

Glycine max

CA783548

8.00E−56

620
G2993

Oryza sativa

CNS08CD7

2.00E−51



( japonica cultivar-



group)

620
G2993

Brassica oleracea

BH972041

1.00E−49

620
G2993

Medicago

AG139600

4.00E−46




truncatula


620
G2993

Lotus corniculatus

AP006401

2.00E−45



var. japonicus

620
G2993

Flaveria bidentis

FB118579

4.00E−45

620
G2993

Vitis aestivalis

CB289369

5.00E−44

620
G2993

Lotus japonicus

AP004968

5.00E−44

620
G2993

Zea mays

CC613855

1.00E−43

620
G2993
Oryza sativa ( indica
AAAA01010656

1.00E−42



cultivar-group)

620
G2993

Flaveria bidentis

gi13374061

5.60E−43

620
G2993

Oryza sativa

gi5091602

1.20E−40

620
G2993

Oryza sativa

gi32480091

5.00E−35



( japonica cultivar-



group)

620
G2993

Flaveria trinervia

gi13277216

2.50E−32

620
G2993

Lactuca sativa

gi29119890

3.00E−19

620
G2993

Zea mays

gi6016218

0.17

620
G2993

Malus domestica

gi1946218

0.28

620
G2993

Pisum sativum

gi23504755

0.68

620
G2993

Lycopersicon

gi1418988

0.77




esculentum


620
G2993
Zea mays subsp.
gi21953540

0.82




mays


622
G2996

Brassica napus

CD826370

7.00E−88

622
G2996

Flaveria bidentis

FB118579

3.00E−57

622
G2996

Brassica oleracea

BZ009581

1.00E−55

622
G2996

Lotus japonicus

AP004968

1.00E−51

622
G2996

Zea mays

CC673594

5.00E−51

622
G2996

Oryza sativa

AK109528

2.00E−49



( japonica cultivar-



group)

622
G2996
Oryza sativa ( indica
AAAA01010656

2.00E−49



cultivar-group)

622
G2996

Glycine max

AW508242

4.00E−46

622
G2996

Oryza sativa

OSJN00063

7.00E−43

622
G2996

Medicago

AC139600

1.00E−41




truncatula


622
G2996

Flaveria bidentis

gi13374061

1.40E−55

622
G2996

Oryza sativa

gi5091602

4.40E−43

622
G2996

Oryza sativa

gi32480091

1.00E−41



( japonica cultivar-



group)

622
G2996

Flaveria trinervia

gi13277216

9.70E−39

622
G2996

Lactuca sativa

gi29119890

8.30E−17

622
G2996

Glycine max

gi347455

9.40E−13

622
G2996

Phaseolus vulgaris

gi81870

5.70E−12

622
G2996

Lycopersicon

gi100215

7.00E−12




esculentum


622
G2996

Vigna unguiculata

gi791150

1.20E−11

622
G2996

Solanum tuberosum

gi24745586

1.30E−11

624
G2998

Brassica oleracea

BH543781

1.00E−108

624
G2998

Brassica napus

CD825397

1.00E−105

624
G2998

Gossypium

BG443964

3.00E−60




arboreum


624
G2998

Lotus corniculatus

AP006401

2.00E−56



var. japonicus

624
G2998

Oryza sativa

CNSO8CD7

3.00E−48



( japonica cultivar-



group)

624
G2998

Glycine max

CA783548

2.00E−46

624
G2998

Zea mays

CC613855

3.00E−46

624
G2998

Flaveria bidentis

FB118579

4.00E−46

624
G2998

Lotus japonicus

AP004968

1.00E−43

624
G2998
Oryza sativa ( indica
AAAA01000157

9.00E−42



cultivar-group)

624
G2998

Oryza sativa

gi5091602

3.10E−43

624
G2998

Oryza sativa

gi19387257

6.40E−42



( japonica cultivar-



group)

624
G2998

Flaveria bidentis

gi13277218

8.00E−39

624
G2998

Flaveria trinervia

gi13277216

3.40E−38

624
G2998

Lactuca sativa

gi29119890

2.10E−20

624
G2998

Nicotiana alata

gi1247386

0.0026

624
G2998

Sporobolus

gi6478148

0.0038




stapfianus


624
G2998

Cucumis sativus

gi3810890

0.0077

624
G2998

Zea mays

gi15321716

0.0086

624
G2998

Chlamydomonas

gi16209575

0.009




reinhardtii


626
G2999

Brassica oleracea

BH686720

4.00E−68

626
G2999

Lotus corniculatus

AP006401

2.00E−55



var. japonicus

626
G2999

Gossypium

BG443964

1.00E−40




arboreum


626
G2999

Flaveria bidentis

FB118579

2.00E−39

626
G2999

Glycine max

CA783548

2.00E−39

626
G2999

Lotus japonicus

AP004968

2.00E−38

626
G2999

Oryza sativa

AK108246

6.00E−38



( japonica cultivar-



group)

626
G2999

Zea mays

CC629064

2.00E−37

626
G2999
Oryza sativa ( indica
AAAA01010656

3.00E−36



cultivar-group)

626
G2999

Brassica napus

CD835782

4.00E−36

626
G2999

Flaveria bidentis

gi13277220

1.70E−40

626
G2999

Oryza sativa

gi19387257

1.10E−34



( japonica cultivar-



group)

626
G2999

Oryza sativa

gi5091602

5.10E−33

626
G2999

Flaveria trinervia

gi13277216

3.20E−31

626
G2999

Lactuca sativa

gi29119890

1.10E−16

626
G2999

Asarum europaeum

gi8163936

0.92

626
G2999

Mesembryanthemum

gi3342196

0.98




crystallinum


626
G2999

Nicotiana tabacum

gi18448717

0.99

626
G2999

Calycanthus

gi8163940

1




floridus


628
G3002

Brassica oleracea

BZ508225

5.00E−37

628
G3002

Vitis vinifera

CB347557

3.00E−25

628
G3002

Glycine max

BM886058

4.00E−24

628
G3002

Brassica napus

CD818854

1.00E−23

628
G3002

Zea mays

CC675142

3.00E−21

628
G3002

Sorghum bicolor

AF369906

3.00E−20

628
G3002

Lycopersicon

AW092295

4.00E−19




esculentum


628
G3002

Lotus corniculatus

AP006401

6.00E−19



var. japonicus

628
G3002

Oryza sativa

AK111350

1.00E−18



( japonica cultivar-



group)

628
G3002

Oryza sativa

10A19I

1.00E−18

628
G3002

Oryza sativa

gi28301934

1.30E−25



( japonica cultivar-



group)

628
G3002

Oryza sativa

gi5091602

1.90E−23

62S
G3002

Flaveria bidentis

gi13277218

2.90E−22

628
G3002

Flaveria trinervia

gi13277216

9.90E−14

628
G3002

Lactuca sativa

gi29119890

7.40E−09

628
G3002

Picea mariana

gi2982264

0.68

628
G3002

Chloroplast

gi2735522

0.85




Schismus barbatus


628
G3002

Chloroplast

gi2735530

0.95




Rytidosperma





pumilum


628
G3002

Lycopersicon

gi12231300

0.96




esculentum


628
G3002

Zea mays

gi477226

0.98

630
G3003

Glycine max

GMA311808

8.00E−83

630
G3003

Helianthus annuus

CD849311

7.00E−81

630
G3003

Medicago

CA921097

1.00E−79




truncatula


630
G3003

Oryza sativa

AK071104

2.00E−78



( japonica cultivar-



group)

630
G3003

Brassica oleracea

BH986891

8.00E−78

630
G3003

Gossypium

BQ407338

6.00E−75




arboreum


630
G3003

Populus tremula x

BU825617

1.00E−74




Populus tremuloides


630
G3003

Triticum turgidum

BF293596

4.00E−72

630
G3003

Eschscholzia

CD476634

8.00E−72




californica


630
G3003
Oryza sativa ( indica
AAAA01004319

2.00E−66



cultivar-group)

630
G3003

Glycine max

gi18376601

9.80E−78

630
G3003

Oryza sativa

gi15290024

3.50E−73

630
G3003

Oryza sativa

gi20161636

3.50E−73



( japonica cultivar-



group)

630
G3003

Lycopersicon

gi15984226

2.90E−23




esculentum


630
G3003

Solanum tuberosum

gi563623

4.80E−16

630
G3003

Zea mays

gi3170601

1.00E−11

630
G3003
Fragaria x
gi1362039

0.2



ananassa

630
G3003

Nicotiana tabacum

gi4887016

0.91

630
G3003

Vigna radiata

gi11131101

0.97

630
G3003

Betula pendula

gi30577628

1

632
G3008

Brassica oleracea

BZ501815

1.00E−112

632
G3008

Lotus corniculatus

AP006419

1.00E−104



var. japonicus

632
G3008

Medicago

AC125389

4.00E−97




truncatula


632
G3008

Vigna radiata

AF467783

2.00E−92

632
G3008

Lotus japonicus

AP004499

3.00E−91

632
G3008

Cucumis melo

AB063192

2.00E−90

632
G3008

Lycopersicon

AF328784

8.00E−90




esculentum


632
G3008

Nicotiana tabacum

AB015855

1.00E−88

632
G3008

Zea mays

AX077258

7.00E−86

632
G3008
Oryza sativa ( indica
AAAA01009163

3.00E−85



cultivar-group)

632
G3008

Cucumis melo

gi15425735

8.50E−96

632
G3008

Vigna radiata

gi18643339

1.20E−94

632
G3008

Nicotiana tabacum

gi30016896

1.80E−93

632
G3008

Lycopersicon

gi14280042

9.80E−93




esculentum


632
G3008

Oryza sativa

gi17221605

1.30E−90



( japonica cultivar-



group)

632
G3008

Dianthus

gi7739795

2.30E−87




caryophyllus


632
G3008

Zea mays

gi13121846

2.70E−82

632
G3008

Fagus sylvatica

gi10241607

9.50E−80

632
G3008
Rosa hybrid cultivar-
gi20378359

1.00E−59

632
G3008

Cicer arietinum

gi8894550

9.50E−13

634
G3017

Oryza sativa

AK109662

1.00E−39



( japonica cultivar-



group)

634
G3017

Solanum tuberosum

BQ513392

6.00E−37

634
G3017

Glycine max

AW349686

3.00E−36

634
G3017
Oryza sativa ( indica
AAAA01007394

6.00E−32



cultivar-group)

634
G3017

Oryza sativa

AP003983

3.00E−30

634
G3017

Triticum aestivum

CA654295

4.00E−29

634
G3017

Brassica oleracea

BZ043983

2.00E−26

634
G3017

Zea mays

BH779475

6.00E−26

634
G3017

Physcomitrella

BJ164531

8.00E−18



patens subsp. patens

634
G3017

Hordeum vulgare

BU980391

5.00E−14



subsp. vulgare

634
G3017

Oryza sativa

gi13486646

3.90E−35

634
G3017

Oryza sativa

gi20160648

2.00E−15



( japonica cultivar-



group)

634
G3017

Pinus taeda

gi6166283

3.60E−07

634
G3017

Tulipa gesneriana

gi5923912

4.10E−07

634
G3017

Lycopersicon

gi5669656

1.60E−06




esculentum


634
G3017

Antirrhinum majus

gi166428

3.10E−06

634
G3017

Brassica napus

gi11045087

4.10E−06

634
G3017

Petunia x hybrida

gi10998404

7.90E−05

634
G3017

Glycine max

gi3399777

0.00011

634
G3017

Perilla frutescens

gi4519199

0.00035

636
G3021

Brassica napus

CD834815

3.00E−67

636
G3021

Brassica oleracea

BH955481

8.00E−24

636
G3021

Medicago

AC125475

3.00E−22




truncatula


636
G3021

Gossypium

BQ411429

9.00E−12




arboreum


636
G3021

Glycine max

CD394118

2.00E−11

636
G3021

Gossypium

AI727885

2.00E−08




hirsutum


636
G3021

Prunus persica

BU043443

5.00E−08

636
G3021

Solanum tuberosum

BM110947

6.00E−08

636
G3021

Vitis vinifera

CD798414

1.00E−05

636
G3021

Cycas rumphii

CB092407

2.00E−05

636
G3021

Oryza sativa

gi20160528

0.00013



( japonica cultivar-



group)

636
G3021

Oryza sativa

gi15528743

0.0017

636
G3021

Chlamydomonas

gi895614

0.82




reinhardtii


636
G3021

Pinus taeda

gi6166283

0.83

636
G3021

Triticum aestivum

gi100802

0.92

636
G3021

Prunus armeniaca

gi2677828

1

636
G3021

Hordeum vulgare

gi4105117

1

638
G3032

Hordeum vulgare

BF256704

6.00E−14

638
G3032

Brassica oleracea

BH466839

1.00E−13

638
G3032

Oryza sativa

AP004872

2.00E−13



( japonica cultivar-



group)

638
G3032

Chlamydomonas

AF309494

4.00E−13




reinhardtii


638
G3032

Gossypium

AW726892

9.00E−13




arboreum


638
G3032

Oryza sativa

CA757143

4.00E−12

638
G3032

Sorghum

BZ694881

4.00E−12




propinquum


638
G3032
Oryza sativa ( indica
AAAA01004665

1.00E−11



cultivar-group)

638
G3032

Triticum aestivum

CA743799

2.00E−11

638
G3032

Zea mays

ZMEXTENS

4.00E−11

638
G3032

Chlamydomonas

gi12018147

6.50E−16




reinhardtii


638
G3032

Zea mays

gi1076802

8.30E−12

638
G3032

Glycine max

gi99897

1.00E−11

638
G3032

Volvox carteri f.

gi6523547

2.60E−11




nagariensis


638
G3032

Santalum album

gi2429362

1.30E−09

638
G3032

Nicotiana tabacum

gi119714

4.70E−09

638
G3032

Oryza sativa

gi32488576

2.70E−08



( japonica cultivar-



group)

638
G3032

Oryza sativa

gi12083527

3.00E−08

638
G3032

Nicotiana alata

gi2653671

3.40E−08

638
G3032

Zea diploperennis

gi228938

4.70E−08

640
G3044

Lotus corniculatus

CB828026

6.00E−45



var. japonicus

640
G3044

Oryza sativa

AK103853

7.00E−44



( japonica cultivar-



group)

640
G3044

Avicennia marina

BM497415

3.00E−42

640
G3044

Hordeum vulgare

CA015610

3.00E−42



subsp. vulgare

640
G3044

Medicago

B0644829

5.00E−42




truncatula


640
G3044

Triticum aestivum

BJ267378

1.00E−41

640
G3044

Hordeum vulgare

BE455695

7.00E−41

640
G3044

Glycine max

BU765737

3.00E−40

640
G3044

Triticum

BF200249

4.00E−40




monococcum


640
G3044

Solanum tuberosum

BG887287

7.00E−40

640
G3044

Oryza sativa

gi20804997

4.20E−45



( japonica cultivar-



group)

640
G3044

Oryza sativa

gi11862964

1.00E−41

640
G3044

Tulipa gesneriana

gi5923912

2.30E−37

640
G3044

Pinus taeda

gi5923912

2.30e−37

640
G3044

Gossypium

gi13346180

4.60E−05




hirsutum


640
G3044

Oryza rufipogon

gi1086538

8.60E−05

640
G3044

Phyllostachys acuta

gi527661

0.00022

640
G3044

Lycopersicon

gi6175252

0.00027




esculentum


640
G3044

Glycine max

gi3399777

0.00039

640
G3044

Sorghum bicolor

gi527665

0.00052

642
G3054

Brassica oleracea

BZ041399

3.00E−37

642
G3054

Brassica napus

CD836802

3.00E−36

642
G3054

Brassica rapa

BQ791259

4.00E−36



subsp. pekinensis

642
G3054

Oryza sativa

AP004635

9.00E−25

642
G3054
Oryza sativa ( indica
AAAA01000844

9.00E−25



cultivar-group)

642
G3054

Triticum aestivum

CD861617

6.00E−24

642
G3054

Lycopersicon

AW930486

1.00E−22




esculentum


642
G3054

Trifolium

BU576932

1.00E−20




purpureum


642
G3054

Lotus japonicus

BI417981

2.00E−20

642
G3054

Triticum

BQ801216

3.00E−20




monococcum


642
G3054

Oryza sativa

gi29467558

6.40E−20



( japonica cultivar-



group)

642
G3054

Pisum sativum

gi14018368

2.50E−15

642
G3054

Oryza sativa

gi5441893

2.20E−10

642
G3054

Pinus pinaster

gi18129298

3.60E−05

642
G3054

Hordeum vulgare

gi3153151

0.63



subsp. vulgare

642
G3054

Hordeum vulgare

gi7443232

0.63

643
G3055

Glycine max

GLYMA-28NOV01-
1337




CLUSTER35081_1

643
G3055

Glycine max

GLYMA-28NOV01-
1338




CLUSTER35081_2

643
G3055

Oryza sativa

LIB4831-032-R1-N1-E4
1339

643
G3055

Oryza sativa

ORYSA-22JAN02-
1340




CLUSTER3268_1

643
G3055

Zea mays

701166667H1
1341

643
G3055

Zea mays

ZEAMA-08NOV01-
1342




CLUSTER22810_1

643
G3055

Zea mays

ZEAMA-08NOV01-
1343




CLUSTER22810_2

643
G3055

Zea mays

ZEAMA-08NOV-1-
1344




CLUSTER22810_3

643
G3055

Zea mays

ZEAMA-08NOV01-
1345




CLUSTER22810_4

643
G3055

Glycine max

Gma_S5098126
1686

643
G3055

Zea mays

Zm_S11417368
1832

643
G3055

Triticum aestivum

Ta_S159757
1922

643
G3055

Lycopersicon

SGN-UNIGENE-
2097




esculentum

SINGLET-330143

643
G3055

Brassica oleracea

BZ078892

2.00E−95

643
G3055
Oryza sativa ( indica
AAAA01000844

1.00E−80



cultivar-group)

643
G3055

Oryza sativa

AP004635

1.00−80

643
G3055

Triticum

BQ801216

1.00E−71




monococcum


643
G3055

Brassica napus

CD836802

2.00E−71

643
G3055

Sorghum bicolor

BE593461

2.00E−67

643
G3055

Brassica rapa

BQ791259

8.00E−64



subsp. pekinensis

643
G3055

Lycopersicon

AW930486

3.00E−58




esculentum


643
G3055

Hordeum vulgare

BU971385

1.00E−57



subsp. vulgare

643
G3055

Zea mays

CC372422

3.00E−56

643
G3055

Oryza sativa

gi29467558

1.80−99



( japonica cultivar-



group)

643
G3055

Pisum sativum

gi14018368

2.50E−32

643
G3055

Oryza sativa

gi5441893

2.30E−10

643
G3055

Citrus unshiu

gi7024449

1.10E−08

643
G3055

Zea mays

gi22293

1.30E−08

643
G3055

Sorghum bicolor

gi21623

3.70E−08

643
G3055

CIcer arietinum

gi31068672

1.30E−07

643
G3055

Hordeum vulgare

gi1229138

5.70E−07

643
G3055

Lycopersicon

gi1166450

8.50E−07




esculentum


643
G3055

Nicotiana tabacum

gi395147

1.50E−06

646
G3059

Vitis vinifera

CB349575

7.00E−59

646
G3059

Brassica oleracea

BH714891

1.00E−55

646
G3059

Oryza sativa

AK108658

6.00E−50



( japonica cultivar-



group)

646
G3059
Oryza sativa ( indica
AAAA01001811

2.00E−45



cultivar-group)

646
G3059

Oryza sativa

AC098571

2.00E−45

646
G3059

Zea mays

CC169635

1.00E−38

646
G3059

Hordeum vulgare

BI958345

1.00E−36



subsp. vulgare

646
G3059

Populus

AI166416

2.00E−21



balsaifera subsp.




trichocarpa


646
G3059

Sorghum bicolor

CF073535

6.00E−19

646
G3059

Medicago

AW329637

1.00E−12




truncatulaA


646
G3059

Oryza sativa

gi13603429

8.50E−53

646
G3059

Oryza sativa

gi29467558

2.70E−08



( japonica cultivar-



group)

646
G3059

Spinacia oleracea

gi2815305

3.40E−06

646
G3059

Pisum sativum

gi31580864

0.00044

646
G3059

Pinus pinaster

gi18129298

0.0015

646
G3059

Petroselinum

gi1169081

0.016




crispum


646
G3059

Hordeum vulgare

gi1418972

0.027

646
G3059

Nicotiana tabacum

gi1076624

0.047

646
G3059
Triticum sp.
gi100838

0.074

646
G3059

Triticum aestivum

gi1199790

0.074

648
G3060

Brassica oleracea

BH549078

2.00E−88

648
G3060

Brassica napus

CD822240

2.00E−76

648
G3060

Zea mays

AY104443

2.00E−62

648
G3060

Lotus corniculatus

AP006377

9.00E−60



var. japonicus

648
G3060
Oryza sativa ( indica
AAAA010099S9

5.00E−53



cultivar-group)

648
G3060

Oryza sativa

AP000367

5.00E−53

648
G3060

Solanum tuberosum

BQ505464

5.00E−52

648
G3060

Glycine max

AW348431

3.00E−49

648
G3060

Oryza sativa

AP003609

3.00E−48



( japonica cultivar-



group)

648
G3060

Triticum aestivum

BF472969

2.00E−47

648
G3060

Oryza sativa

gi32488659

5.10E−49



( japonica cultivar-



group)

648
G3060

Oryza sativa

gi5441893

2.30E−46

648
G3060

Pisum sativum

gi14018368

7.40E−10

648
G3060

Pinus pinaster

gi18129298

2.90E−07

648
G3060

Lycopersicon

gi1345534

0.00084




esculentum


648
G3060

Gossypium

gi126075

0.11




hirsutum


648
G3060

Zea mays

gi22293

0.11

648
G3060

Petunia x hybrida

gi121627

0.14

648
G3060
Petunia sp.
gi225181

0.14

648
G3060

Phaseolus vulgaris

gi121632

0.28

650
G3061

Oryza sativa

AK072128

2.00E−85



( japonica cultivar-



group)

650
G3061

Prunus persica

BU039744

4.00E−84

650
G3061

Lycopersicon

BE459738

8.00E−84




esculentum


650
G3061

Solanum tuberosum

BG590535

2.00E−82

650
G3061

Zea mays

AX658844

6.00E−82

650
G3061

Triticum aestivum

CD912590

3.00E−81

650
G3061

Robinia

BI677739

7.00E−81




pseudoacacia


650
G3061

Capsicum annuum

BM063853

2.00E−79

650
G3061

Brassica oleracea

BZ520039

2.00E−79

650
G3061

Medicago

BG645203

3.00E−79




truncatula


650
G3061

Oryza sativa

gi21741797

8.90E−80



( japonica cultivar-



group)

650
G3061

Solanum tuberosum

gi563623

1.90E−79

650
G3061

Oryza sativa

gi10934090

6.10E−77

650
G3061

Lycopersicon

gi9858780

1.70E−74




esculentum


650
G3061

Zea mays

gi3170601

1.20E−71

650
G3061

Glycine max

gi18376601

2.30E−19

650
G3061

Triticum aestivum

gi485814

0.67

650
G3061

Petunia x hybrida

gi2346986

0.95

650
G3061

Tsuga canadensis

gi4530513

1

651
G3067

Medicago

Mtr_S5406390
1716




truncatula


651
G3067

Medicago

Mtr_S7093057
1717




truncatula


651
G3067

Zea mays

Zm_S11388015
1833

651
G3067

Lycopersicon

SGN-UNIGENE-56587
2098




esculentum


651
G3067

Lycopersicon

SGN-UNIGENE-
2099




esculentum

SINGLET-360212

652
G3067

Oryza sativa

AK104311

1.00E−127



( japonica cultivar-



group)

652
G3067

Oryza sativa

AP003766

1.00E−105

652
G3067
Oryza sativa ( indica
AAAA01011671

1.00E−102



cultivar-group)

652
G3067

Medicago

AC144502

2.00E−92




truncatula


652
G3067

Lactuca sativa

BQ849595

5.00E−86

652
G3067

Zea mays

CC705079

2.00E−78

652
G3067

Glycine max

BG352898

3.00E−76

652
G3067

Brassica oleracea

BZ503751

1.00E−71

652
G3067

Hedyotis

CB087520

2.00E−71




centranthoides


652
G3067

Hordeum vulgare

CA030376

4.00E−69



subsp. vulgare

652
G3067

Oryza sativa

gi20804915

2.70E−122



( japonica cultivar-



group)

652
G3067

Oryza sativa

gi5777616

3.60E−56

652
G3067

Nicotiana tabacum

gi27529852

0.55

652
G3067

Petunia x hybrida

gi20563

0.72

652
G3067

Lycopersicon

gi122003

0.82




esculentum


652
G3067

Chloroplast

gi21309725

1




Phaseolus vulgaris


652
G3067

Glycine max

gi2129831

1

653
G3070

Triticum aestivum

Ta_S122753
1923

654
G3070

Brassica oleracea

BH986969

2.00E−72

654
G3070

Oryza sativa

AK104311

6.00E−50



( japonica cultivar-



group)

654
G3070

Hordeum vulgare

CA030376

2.00E−42



subsp. vulgare

654
G3070
Oryza sativa ( indica
AAAA01000203

4.00E−42



cultivar-group)

654
G3070

Oryza sativa

AP003406

4.00E−42

654
G3070

Zea mays

CC705079

1.00E−40

654
G3070

Medicago

AC144502

9.00E−40




truncatula


654
G3070

Lactuca sativa

BQ849595

4.00E−38

654
G3070

Hedyotis

CB087520

7.00E−37




centranthoides


654
G3070

Glycine max

BG352898

7.00E−35

654
G3070

Oryza sativa

gi20804915

7.50E−58



( japonica cultivar-



group)

654
G3070

Oryza sativa

gi5777616

1.50E−24

654
G3070

Zea mays

gi1504058

0.88

654
G3070

Chlorella vulgaris

gi2224518

0.97

654
G3070

Chloroplast

gi7520715

0.97




Chlorella vulgaris


654
G3070

Medicago sativa

gi913595

1

654
G3070

Brassica napus

gi2809204

1

655
G3076

Oryza sativa

Os_S95874
1630

655
G3076

Lycopersicon

SGN-UNIGENE-52322
2100




esculentum


656
G3076

Brassica oleracea

BH458827

1.00E−59

656
G3076

Lycopersicon

AI489100

3.00E−52




esculentum


656
G3076

Theobroma cacao

CA796492

6.00E−31

656
G3076
Nicotiana glauca X
TOBTID3

3.00E−25




Nicotiana





langsdorffii


656
G3076

Populus tremula x

BU866131

3.00E−21




Populus tremuloides


656
G3076

Medicago

BQ123004

4.00E−20




truncatula


656
G3076

Zea mays

CC633595

8.00E−18

656
G3076

Oryza sativa

AK106334

1.00E−17



( japonica cultivar-



group)

656
G3076

Oryza sativa

AP003567

4.00E−17

656
G3076
Oryza sativa ( indica
AAAA01001312

4.00E−17



cultivar-group)

656
G3076

Oryza sativa

gi15408613

1.10E−19

656
G3076

Oryza sativa

gi21104797

1.10E−19



( japonica cultivar-



group)

656
G3076

Lycopersicon

gi4959970

4.30E−13




esculentum


656
G3076

Triticum aestivum

gi100809

2.70E−12

656
G3076

Solanum tuberosum

gi13195751

6.90E−12

656
G3076

Zea mays

gi297020

8.80E−12

656
G3076
Nicotiana glauca X
gi688423

1.00E−11




Nicotiana





langsdorffii


656
G3076

Phaseolus vulgaris

gi15148924

1.40E−10

656
G3076

Nicotiana tabacum

gi12230709

1.10E−09

656
G3076

Glycine max

gi7488719

1.40E−08

657
G3083

Oryza sativa

LIB3434-065-P1-K1-B5
1346

657
G3083

Oryza sativa

Os_S54214
1631

657
G3083

Glycine max

Gma_S4880456
1687

657
G3083

Hordeum vulgare

Hv_S60182
1753

657
G3083

Triticum aestivum

Ta_S179586
1924

657
G3083

Lycopersicon

SGN-UNIGENE-
2101




esculentum

SINGLET-306367

658
G3083

Medicago

BQ123004

9.00E−65




truncatula


658
G3083

Arachis hypogaea

CD038559

3.00E−58

658
G3083

Glycine max

BE657440

7.00E−51

658
G3083

Theobroma cacao

CA794948

2.00E−48

658
G3083

Phaseolus

CA899019

8.00E−47




coccineus


658
G3083

Brassica oleracea

BZ028606

3.00E−42

658
G3083

Brassica napus

CD823868

3.00E−42

658
G3083

Populus tremula x

BU866131

5.00E−36




Populus tremuloides


658
G3083
Oryza sativa ( indica
AAAA01006352

2.00E−32



cultivar-group)

658
G3083
Nicotiana glauca X
TOBTID3

1.00E−31




Nicotiana





langsdorffii


658
G3083
Nicotiana glauca X
gi688423

8.80E−36




Nicotiana




langsdorffii

658
G3083

Oryza sativa

gi8570052

1.30E−29

658
G3083

Lycopersicon

gi4959970

3.10E−17




esculentum


658
G3083

Nicotiana tabacum

gi12230709

7.50E−16

658
G3083

Triticum aestivum

gi100809

1.60E−15

658
G3083

Solanum tuberosum

gi13195751

3.00E−14

658
G3083

Zea mays

gi297020

6.00E−14

658
G3083

Phaseolus vulgaris

gi15148926

1.90E−13

658
G3083
Nicotiana sp.
gi19680

7.30E−13

658
G3083

Glycine max

gi7488719

5.10E−11

660
G3084

Brassica napus

CD819850

8.00E−16

660
G3084

Prunus persica

BU043737

2.00E−15

660
G3084

Oryza sativa

BI118786

4.00E−15

660
G3084

Oryza sativa

AK104501

8.00E−15



( japonica cultivar-



group)

660
G3084

Hordeum vulgare

AV933892

1.00E−14



subsp. vulgare

660
G3084

Oryza minuta

GB214244

2.00E−14

660
G3084

Hordeum vulgare

BI954130

2.00E−14

660
G3084

Beta vulgaris

BQ592350

7.00E−14

660
G3084

Ipomoea nil

BJ566577

2.00E−13

660
G3084

Populus tremula x

PTR306828

4.00E−13




Populus tremuloides


660
G3084

Populus tremula x

gi20269057

3.10E−14




Populus tremuloides


660
G3084

Glycine max

gi114733

1.30E−13

660
G3084

Vigna radiata

gi287566

1.60E−13

660
G3084
Oryza sativa ( indica
gi30962267

2.10E−13



cultivar-group)

660
G3084

Oryza sativa

gi17154533

3.30E−13

660
G3084

Pisum sativum

gi1352058

1.10E−12

660
G3084

Gossypium

gi22531416

6.20E−12




hirsutum


660
G3084

Pinus pinaster

gi17976835

7.70E−12

660
G3084

Pinus taeda

gi32396295

7.70E−12

660
G3084

Oryza sativa

gi31126760

1.30E−11



( japonica cultivar-



group)

661
G3086

Glycine max

GLYMA-28NOV01-
1347




CLUSTER427_57

661
G3086

Glycine max

GLYMA-28NOV01-
1348




CLUSTER427_63

661
G3086

Glycine max

Gma_S4882467
1688

661
G3086

Glycine max

Gma_S4911216
1689

662
G3086

Oryza sativa

AK100106

2.00E−42



( japonica cultivar-



group)

662
G3086

Ipomoea nil

BJ575230

2.00E−39

662
G3086

Medicago

CA920438

4.00E−38




truncatula


662
G3086

Glycine max

BU550331

2.00E−37

662
G3086

Lycopersicon

AW037896

2.00E−36




esculentum


662
G3086

Brassica oleracea

BH718970

2.00E−36

662
G3086

Solanum tuberosum

BQ113088

2.00E−36

662
G3086

Hordeum vulgare

CA014136

1.00E−35



subsp. vulgare

662
G3086
Oryza sativa ( indica
CB634806

2.00E−35



cultivar-group)

662
G3086

Zea mays

CA831862

2.00E−35

662
G3086

Pinus taeda

gi6166283

4.50E−42

662
G3086

Oryza sativa

gi19401700

2.10E−36

662
G3086

Oryza sativa

gi20161021

1.60E−29



( japonica cultivar-



group)

662
G3086

Tulipa gesneriana

gi5923912

1.80E−10

662
G3086

Mesembryanthemum

gi4206118

6.00E−05




crystallinum


662
G3086

Glycine max

gi3399777

0.00017

662
G3086

Brassica napus

gi27650307

0.00025

662
G3086

Gossypium

gi13346182

0.00055




hirsutum


662
G3086

Pennisetum

gi527657

0.0017




glaucum


662
G3086

Perilla frutescens

gi4519199

0.0029

664
G3091

Gossypium

CD486670

2.00E−93




hirsutum


664
G3091

Medicago

CB894060

8.00E−90




truncatula


664
G3091

Solanum tuberosum

BM112649

1.00E−85

664
G3091

Lupinus albus

CA411473

1.00E−85

664
G3091

Vitis vinifera

CB004487

2.00E−85

664
G3091

Glycine max

BQ473916

1.00E−83

664
G3091

Prunus persica

BU041659

2.00E−82

664
G3091

Prunus dulcis

BU574304

4.00E−82

664
G3091

Lactuca sativa

BQ871960

5.00E−82

664
G3091

Lycopersicon

AW220091

7.00E−81




esculentum


664
G3091

Oryza sativa

gi32489345

1.00E−66



( japonica cultivar-



group)

664
G3091

Oryza sativa

gi12643058

6.00E−62

664
G3091

Pisum sativum

gi16117799

9.70E−33

666
G3094

Glycine max

BI969147

2.00E−60

666
G3094

Hordeum vulgare

BQ765415

2.00E−54



subsp. vulgare

666
G3094

Pinus pinaster

BX254443

3.00E−54

666
G3094

Pinus taeda

AW011103

3.00E−50

666
G3094

Pisum sativum

AB045222

7.00E−46

666
G3094

Lactuca sativa

BQ875949

7.00E−46

666
G3094
Populus tremula x
BU830426

2.00E−45




Populus tremuloides


666
G3094

Triticum aestivum

BJ270124

4.00E−45

666
G3094

Ipomoea nil

BJ563336

2.00E−44

666
G3094

Sorghum

BF481787

1.00E−43




propinquum


666
G3094

Pisum sativum

gi16117799

8.40E−49

666
G3094

Oryza sativa

gi32489345

2.00E−29



( japonica cultivar-



group)

666
G3094

Oryza sativa

gi12643058

1.00E−27

666
G3094

Welwitschia

gi17402656

1




mirabilis


666
G3094

Chlorella vulgaris

gi2224452

1

666
G3094

Gnetum africanum

gi29648802

1

666
G3094

Pelargonium

gi9621935

1




mollicomum


668
G3095
Populus tremula x
BU812421

2.00E−89




Populus tremuloides


668
G3095

Populus tremula

BU889637

2.00E−78

668
G3095

Oryza sativa

AK067251

1.00E−64



( japonica cultivar-



group)

668
G3095

Vitis vinifera

CA818053

3.00E−62

668
G3095

Populus

BU870747

3.00E−60



balsamifera subsp.




trichocarpa


668
G3095

Gossypium

BG443078

3.00E−57




arboreum


668
G3095

Medicago

BQ123285

6.00E−57




truncatula


668
G3095

Cryptomeria

BP176029

7.00E−57




japonica


668
G3095

Glycine max

CA800006

2.00E−56

668
G3095

Oryza sativa

AX653892

4.00E−56

668
G3095

Oryza sativa

gi32489345

1.60E−57



( japonica cultivar-



group)

668
G3095

Oryza sativa

gi12643058

1.10E−50

668
G3095

Pisum sativum

gi16117799

3.70E−30

668
G3095

Picea mariana

gi2996158

0.97

668
G3095

Ipomoea nil

gi1064830

1

670
G3111

Brassica oleracea

BH673411

6.00E−80

670
G3111

Medicago

AC126786

1.00E−58




truncatula


670
G3111

Glycine max

BU762572

8.00E−56

670
G3111

Lycopersicon

AW443687

1.00E−55




esculentum


670
G3111

Lotus japonicus

BI418846

3.00E−54

670
G3111

Vitis vinofera

CB919606

3.00E−52

670
G3111

Solanum tuberosum

BI175970

1.00E−48

670
G3111

Mesembryanthemum

BE033932

2.00E−48




crystallinum


670
G3111

Lupinus albus

CA411115

3.00E−46

670
G3111

Stevia rebaudiana

BG522187

5.00E−42

670
G3111

Oryza sativa

gi12039329

1.40E−35

670
G3111

Oryza sativa

gi31433314

1.40E−35



( japonica cultivar-



group)

670
G3111

Hordeum vulgare

gi2894379

7.20E−34

670
G3111

Cucumis melo

gi17016985

7.10E−27

670
G3111

Zea mays

gi18092342

6.30E−19

670
G3111

Nicotiana tabacum

gi12003386

3.60E−16

670
G3111

Hordeum vulgare

gi20152976

1.40E−14



subsp. vulgare

670
G3111

Medicago sativa

gi23451086

2.00E−14

670
G3111

Glycine max

gi22597166

3.80E−12

670
G3111

Pisum sativum

gi4240031

1.60E−08
































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































Table 8 lists sequences discovered to be paralogous to a number of transcription factors of the present invention. The columns headings include, from left to right, the Arabidopsis SEQ ID NO; corresponding Arabidopsis Gene ID (GID) numbers; the GID numbers of the paralogs discovered in a database search; and the SEQ ID NOs assigned to the paralogs.





TABLE 8



Arabidopsis Transcription Factor Genes and Paralogs







Arabidopsis


Paralog
Paralog

Transcription Factor

Arabidopsis

GID
Nucleotide SEQ

SEQ ID NO:
TF GID No
No.
ID NO:








3
G12
G1379
1441



G24
1349

7
G30
G1791
1461



G1792
1463



G1795
1465

9
G46
G1004
1425



G1419
1447



G29
1351



G43
1355

11
G47
G2133
1495

39
G148
G142
33

43
G153
G152
1365



G1760
1459



G860
1419

45
G155
G131
15



G135
21

79
G355
G1994
1491

83
G370
G1995
1493



G2826
1531



G2838
1535



G361
1383



G362
1385

97
G438
G1548
1453



G390
1389



G391
1391



G392
1393

105
G485
G1364
1439



G2345
1501



G481
1395



G482
1397

121
G627
G149
1363

125
G651
G1914
1481



G1973
1485

127
G652
G1335
1435

141
G807
G810
1417

145
G839
G1196
1429

153
G916
G184
1369



G186
1371

155
G926
G2632
1517

159
G961
G2535
1507



G957
157

161
G975
G1387
1443



G2583
1515

163
G1011
G154
1367

171
G1037
G722
1409

181
G1128
G1399
1445

185
G1142
G2659
1521

189
G1206
G1207
1431

199
G1313
G1325
1433

207
G1357
G1452
1451



G512
1401

215
G1412
G759
1413



G773
1415

223
G1451
G990
1423

225
G1452
G1357
1437



G512
1401

233
G1482
G1888
1477

277
G1792
G1791
1461



G1795
1465



G30
7

281
G1797
G1798
283

283
G1798
G1797
281

287
G1816
G225
1375



G226
1377



G2718
507



G682
1407

297
G1840
G1749
1457



G1839
1473

303
G1863
G2334
1499

305
G1893
G1976
1489



G3062
1555

311
G1928
G2664
1523

333
G1995
G2826
1531



G2838
555



G361
1383



G362
1385



G370
1387

341
G2041
G2882
1537

371
G2207
G2199
1497

393
G2334
G1863
303

407
G2432
G736
1411

417
G2457
G2459
1505

419
G2459
G2457
417

425
G2505
G2635
1519

431
G2536
GS11
1399

435
G2550
G2546
1509

441
G2567
G1017
167

483
G2650
G617
1405

505
G2717
G204
1373



G2709
1525

507
G2718
G1816
287



G225
1375



G226
1377



G682
1407

511
G2741
G1435
1449

525
G2768
G600
117

529
G2776
G1652
1455

545
G2826
G1995
333



G2838
555



G361
1383



G362
1385



G370
1387

547
G2830
G2562
1511



G2563
1513



G2828
1533

555
G2838
G1995
333



G2826
545



G361
1383



G362
1385



G370
1387

557
G2839
G1889
1479



G1974
1487



G353
1379



G354
1381

567
G2854
G1940
1483

569
G2859
G2779
533

571
G2865
G2934
595

593
G2933
G2928
1539



G2932
1541

607
G2979
G2980
1547

609
G2981
G2982
1551

611
G2982
G2981
1549

615
G2990
G2989
1553

651
G3067
G2966
1545





















































































































































Table 9 lists the gene identification number (GID) and relationships for homologous (found using analyses according to Example IX) and variant sequences for the sequences of the Sequence Listing.





TABLE 9



Similarity relationships found within the Sequence Listing







SEQ ID

DNA or
Species from which


NO:
GID
Protein (PRT)
Sequence is Derived
Relationship









671

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

672

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

673

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

674

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

675

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

676

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

677

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

678

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G12

679

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G12

680

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G12

681

PRT

Oryza sativa

Orthologous to G12

682

PRT

Oryza sativa

Orthologous to G12

683

PRT

Oryza sativa

Orthologous to G12

684

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G12

685

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G30, G1792

686

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G30, G1792

687

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G30, G1792

688

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G30, G1792

689

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G30, G1792

690

PRT

Oryza sativa

Orthologous to G30, G1792

691

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G30, G1792

692

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

693

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

694

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

695

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

696

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

697

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

698

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

699

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G46

700

PRT

Oryza sativa

Orthologous to G46

701

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G46

702

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G47

703

PRT

Oryza sativa

Orthologous to G47

704

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G148

705

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G148

706

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G148

707

PRT

Oryza sativa

Orthologous to G148

708

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

709

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

710

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

711

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

712

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

713

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G153

714

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G153

715

PRT

Oryza sativa

Orthologous to G153

716

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153

717

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153

718

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153

719

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

720

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

721

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

722

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

723

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

724

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

725

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

726

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

727

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G155

728

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G155

729

PRT

Oryza sativa

Orthologous to G155

730

PRT

Oryza sativa

Orthologous to G155

731

PRT

Oryza sativa

Orthologous to G155

732

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

733

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

734

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

735

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

736

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

737

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

738

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

739

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

740

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

741

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G200

742

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G200

743

PRT

Oryza sativa

Orthologous to G200

744

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G200

745

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

746

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

747

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

748

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

749

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

750

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

751

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

752

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

753

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

754

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

755

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

756

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

757

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

758

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G370, G2826

759

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

760

PRT

Oryza sativa

Orthologous to G370, G1995, G2826, G2838

761

PRT

Otyza sativa

Orthologous to G370, G1995, G2826, G2838

762

PRT

Oryza sativa

Orthologous to G370, G1995, G2826, G2838

763

PRT

Oryza sativa

Orthologous to G370, G1995, G2826, G2838

764

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

765

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

766

PRT

Oryza sativa

Orthologous to G372

767

PRT

Oryza sativa

Orthologous to G372

768

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

769

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

770

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

771

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

772

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

773

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

774

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

775

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

776

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

777

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

778

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

779

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

780

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

781

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

782

PRT

Oryza sativa

Orthologous to G438

783

PRT

Oryza sativa

Orthologous to G438

784

PRT

Oryza sativa

Orthologous to G438

785

PRT

Oryza sativa

Orthologous to G438

786

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

787

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

788

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

789

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

790

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

791

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

792

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

793

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

794

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

795

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

796

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

797

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

798

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

799

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

800

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

801

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

802

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

803

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

804

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

805

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

806

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

807

PRT

Oryza sativa

Orthologous to G485

808

PRT

Oryza sativa

Orthologous to G485

809

PRT

Oryza sativa

Orthologous to G485

810

PRT

Oryza sativa

Orthologous to G485

811

PRT

Oryza sativa

Orthologous to G485

812

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485

813

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

814

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

815

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

816

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

817

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

818

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

819

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

820

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

821

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

822

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G627

823

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G627

824

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G627

825

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G651

826

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G651

827

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G651

828

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G651

829

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G651

830

PRT

Oryza sativa

Orthologous to G651

831

PRT

Oryza sativa

Orthologous to G651

832

PRT

Oryza sativa

Orthologous to G651

833

PRT

Oryza sativa

Orthologous to G651

834

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G651

835

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

836

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

837

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

838

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

839

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

840

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

841

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

842

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

843

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G652

844

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G652

845

PRT

Oryza sativa

Orthologous to G652

846

PRT

Oryza sativa

Orthologous to G652

847

PRT

Oryza sativa

Orthologous to G652

848

PRT

Oryza sativa

Orthologous to G652

849

PRT

Oryza sativa

Orthologous to G652

850

PRT

Oryza sativa

Orthologous to G652

851

PRT

Oryza sativa

Orthologous to G652

852

PRT

Oryza sativa

Orthologous to G652

853

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G652

854

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

855

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

856

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

857

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

858

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

859

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

860

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

861

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G807

862

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G807

863

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G807

864

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G807

865

PRT

Oryza sativa

Orthologous to G807

866

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G807

867

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G807

868

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G839

869

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G839

870

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G839

871

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G839

872

PRT

Oryza sativa

Orthologous to G839

873

PRT

Oryza sativa

Orthologous to G839

874

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G839

875

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G839

876

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G839

877

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

878

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

879

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

880

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

881

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

882

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

883

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

884

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

885

PRT

Oryza sativa

Orthologous to G916

886

PRT

Oryza sativa

Orthologous to G916

887

PRT

Oryza sativa

Orthologous to G916

888

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G916

889

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G926

890

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G926

891

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G926

892

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G926

893

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G926

894

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G926

895

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G961

896

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G961

897

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G961

898

PRT

Oryza sativa

Orthologous to G961

899

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G961

900

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G961

901

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G961

902

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G975

903

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G975

904

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G975

905

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G975

906

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G975

907

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G975

908

PRT

Oryza sativa

Orthologous to G975

909

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G975

910

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G975

911

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G975

912

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

913

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

914

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

915

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

916

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

917

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

918

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1011

919

PRT

Oryza sativa

Orthologous to G1011

920

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

921

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

922

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

923

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

924

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

925

PRT

Oryza sativa

Orthologous to G1013

926

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1037

927

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1037

928

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1037

929

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1037

930

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1037

931

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1037

932

PRT

Oryza sativa

Orthologous to G1037

933

PRT

Oryza sativa

Orthologous to G1037

934

PRT

Oryza sativa

Orthologous to G1037

935

PRT

Oryza sativa

Orthologous to G1037

936

PRT

Oryza sativa

Orthologous to G1037

937

PRT

Oryza sativa

Orthologous to G1037

938

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1037

939

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1037

940

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1037

941

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1037

942

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1128

943

PRT

Oryza sativa

Orthologous to G1128

944

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1142

945

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1142

946

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1142

947

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1142

948

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

949

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

950

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

951

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

952

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

953

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1206

954

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1206

955

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1206

956

PRT

Oryza sativa

Orthologous to G1206

957

PRT

Oryza sativa

Orthologous to G1206

958

PRT

Oryza sativa

Orthologous to G1206

959

PRT

Oryza sativa

Orthologous to G1206

960

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1206

961

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

962

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

963

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

964

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

965

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

966

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

967

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

968

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1274

969

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1274

970

PRT

Oryza sativa

Orthologous to G1274

971

PRT

Oryza sativa

Orthologous to G1274

972

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1274

973

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1274

974

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1274

975

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1274

976

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1313

977

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1313

978

PRT

Oryza sativa

Orthologous to G1313

979

PRT

Oryza sativa

Orthologous to G1313

980

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1313

981

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1313

982

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1357, G1452

983

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

984

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

985

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

986

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

987

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

988

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

989

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

990

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

991

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1412

992

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1420

993

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1420

994

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1420

995

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1420

996

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1451

997

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1451

998

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1451

999

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1451

1000

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1451

1001

PRT

Oryza sativa

Orthologous to G1451

1002

PRT

Oryza sativa

Orthologous to G1451

1003

PRT

Oryza sativa

Orthologous to G1451

1004

PRT

Oryza sativa

Orthologous to G1451

1005

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1006

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1007

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1008

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1009

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1468

1010

PRT

Oryza sativa

Orthologous to G1468

1011

PRT

Oryza sativa

Orthologous to G1468

1012

PRT

Oryza sativa

Orthologous to G1468

1013

PRT

Oryza sativa

Orthologous to G1476

1014

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1482

1015

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1482

1016

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1482

1017

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1482

1018

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1482

1019

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1482

1020

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1482

1021

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1482

1022

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1482

1023

PRT

Oryza sativa

Orthologous to G1482

1024

PRT

Oryza sativa

Orthologous to G1482

1025

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1026

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1027

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1028

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1029

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1030

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1031

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1510

1032

PRT

Oryza sativa

Orthologous to G1510

1033

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1539

1034

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1539

1035

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1557

1036

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1660

1037

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1660

1038

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1660

1039

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1660

1040

PRT

Oryza sativa

Orthologous to G1660

1041

PRT

Oryza sativa

Orthologous to G1660

1042

PRT

Oryza sativa

Orthologous to G1660

1043

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1044

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1045

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1046

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1047

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1048

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1730

1049

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1753

1050

PRT

Oryza sativa

Orthologous to G1753

1051

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1779

1052

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1779

1053

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1779

1054

PRT

Oryza sativa

Orthologous to G1779

1055

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1779

1056

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1796

1057

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1816, G2718

1058

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1816, G2718

1059

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1816, G2718

1060

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1816, G2718

1061

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1816, G2718

1062

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1816

1063

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1816, G2718

1064

PRT

Oryza sativa

Orthologous to G1816, G2718

1065

PRT

Oryza sativa

Orthologous to G1816, G2718

1066

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1816, G2718

1067

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1816, G2718

1068

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1893

1069

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1893

1070

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1893

1071

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1893

1072

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1893

1073

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1893

1074

PRT

Oryza sativa

Orthologous to G1893

1075

PRT

Oryza sativa

Orthologous to G1893

1076

PRT

Oryza sativa

Orthologous to G1893

1077

PRT

Oryza sativa

Orthologous to G1893

1078

PRT

Oryza sativa

Orthologous to G1893

1079

PRT

Oryza sativa

Orthologous to G1893

1080

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1893

1081

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1893

1082

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1928

1083

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1928

1084

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1928

1085

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1928

1086

PRT

Oryza sativa

Orthologous to G1928

1087

PRT

Oryza sativa

Orthologous to G1928

1088

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1928

1089

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1928

1090

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1968

1091

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1968

1092

PRT

Oryza sativa

Orthologous to G1968

1093

PRT

Oryza sativa

Orthologous to G1968

1094

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1983

1095

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1983

1096

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1097

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1985

1098

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1988

1099

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1988

1100

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1988

1101

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1988

1102

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1988

1103

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1988

1104

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1988

1105

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2041

1106

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2041

1107

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2041

1108

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2085

1109

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2085

1110

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2085

1111

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2085

1112

PRT

Oryza sativa

Orthologous to G2085

1113

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2085

1114

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2109

1115

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2109

1116

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1117

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1118

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1119

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1120

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1121

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1122

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2142

1123

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2142

1124

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2142

1125

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2239

1126

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2239

1127

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2239

1128

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2239

1129

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2239

1130

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2239

1131

PRT

Oryza sativa

Orthologous to G2239

1132

PRT

Oryza sativa

Orthologous to G2239

1133

PRT

Oryza sativa

Orthologous to G2239

1134

PRT

Oryza sativa

Orthologous to G2239

1135

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2239

1136

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2239

1137

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2317

1138

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2317

1139

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2319

1140

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2319

1141

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2319

1142

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2319

1143

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2432

1144

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2432

1145

PRT

Oryza sativa

Orthologous to G2432

1146

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2453

1147

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2457, G2459

1148

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2457, G2459

1149

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2457, G2459

1150

PRT

Oryza sativa

Orthologous to G2457, G2459

1151

PRT

Oryza sativa

Orthologous to G2457, G2459

1152

PRT

Oryza sativa

Orthologous to G2457, G2459

1153

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2457, G2459

1154

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2457

1155

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2457

1156

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2457

1157

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2457

1158

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2459

1159

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2459

1160

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2459

1161

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2505

1162

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2505

1163

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2536

1164

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2536

1165

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2536

1166

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2536

1167

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2536

1168

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2536

1169

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2536

1170

PRT

Oryza sativa

Orthologous to G2536

1171

PRT

Oryza sativa

Orthologous to G2536

1172

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2536

1173

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2550

1174

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2550

1175

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2550

1176

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1177

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1178

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1179

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1180

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1181

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1182

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2567

1183

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2567

1184

PRT

Oryza sativa

Orthologous to G2567

1185

PRT

Oryza sativa

Orthologous to G2567

1186

PRT

Oryza sativa

Orthologous to G2567

1187

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2567

1188

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2567

1189

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2567

1190

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2567

1191

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2567

1192

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2571

1193

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2571

1194

PRT

Oryza sativa

Orthologous to G2571

1195

PRT

Oryza sativa

Orthologous to G2579

1196

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2579

1197

PRT

Oryza sativa

Orthologous to G2585

1198

PRT

Oryza sativa

Orthologous to G2585

1199

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2585

1200

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2617

1201

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2650

1202

PRT

Oryza sativa

Orthologous to G2650

1203

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2650

1204

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2650

1205

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2650

1206

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2650

1207

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2650

1208

PRT

Oryza sativa

Orthologous to G2661

1209

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1210

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1211

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1212

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1213

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1214

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1215

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1216

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2717

1217

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2717

1218

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2717

1219

PRT

Oryza sativa

Orthologous to G2717

1220

PRT

Oryza sativa

Orthologous to G2717

1221

PRT

Oryza sativa

Orthologous to G2717

1222

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1223

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1224

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1225

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1226

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1227

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1228

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2723

1229

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2741

1230

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2741

1231

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2741

1232

PRT

Oryza sativa

Orthologous to G2741

1233

PRT

Oryza sativa

Orthologous to G2741

1234

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2741

1235

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2741

1236

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2741

1237

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2754

1238

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2754

1239

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2754

1240

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2754

1241

PRT

Oryza sativa

Orthologous to G2754

1242

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2754

1243

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2754

1244

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2768

1245

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2768

1246

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2768

1247

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2768

1248

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2768

1249

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2768

1250

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2768

1251

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2768

1252

PRT

Oryza sativa

Orthologous to G2768

1253

PRT

Oryza sativa

Orthologous to G2768

1254

PRT

Oryza sativa

Orthologous to G2768

1255

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1256

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1257

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1258

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1259

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1260

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1261

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2768

1262

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1263

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1264

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1265

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1266

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1267

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1268

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1269

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1270

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1271

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2776

1272

PRT

Oryza sativa

Orthologous to G2776

1273

PRT

Oryza sativa

Orthologous to G2776

1274

PRT

Oryza sativa

Orthologous to G2776

1275

PRT

Oryza sativa

Orthologous to G2776

1276

PRT

Oryza sativa

Orthologous to G2776

1277

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2776

1278

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2776

1279

PRT

Oryza sativa

Orthologous to G2826, G2838

1280

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2830

1281

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2839

1282

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2839

1283

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2839

1284

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2839

1285

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2839

1286

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2839

1287

PRT

Oryza sativa

Orthologous to G2839

1288

PRT

Oryza sativa

Orthologous to G2839

1289

PRT

Oryza sativa

Orthologous to G2839

1290

PRT

Oryza sativa

Orthologous to G2839

1291

PRT

Oryza sativa

Orthologous to G2839

1292

PRT

Oryza sativa

Orthologous to G2839

1293

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1294

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1295

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1296

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1297

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1298

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1299

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2839

1300

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2854

1301

PRT

Oryza sativa

Orthologous to G2854

1302

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2854

1303

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2859

1304

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2865

1305

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2865

1306

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2885

1307

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2907

1308

PRT

Oryza sativa

Orthologous to G2907

1309

PRT

Oryza sativa

Orthologous to G2907

1310

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2907

1311

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2907

1312

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2913

1313

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2913

1314

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2933

1315

PRT

Oryza sativa

Orthologous to G2933

1316

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2933

1317

PRT

Oryza sativa

Orthologous to G2969

1318

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1319

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1320

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1321

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1322

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1323

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1324

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981

1325

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1326

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981, G2982

1327

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2981, G2982

1328

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2983

1329

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2983

1330

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2983

1331

PRT

Oryza sativa

Orthologous to G2983

1332

PRT

Oryza sativa

Orthologous to G2983

1333

PRT

Oryza sativa

Orthologous to G2983

1334

PRT

Oryza sativa

Orthologous to G2990

1335

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2990

1336

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2990

1337

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3055

1338

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3055

1339

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G3055

1340

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G3055

1341

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3055

1342

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3055

1343

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3055

1344

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3055

1345

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3055

1346

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G3083

1347

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3086

1348

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3086

1349
G24
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G12

1350
G24
PRT

Arabidopsis thaliana

Paralogous to G12

1351
G29
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G46

1352
G29
PRT

Arabidopsis thaliana

Paralogous to G46

1353
G30
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1792

1354
G30
PRT

Arabidopsis thaliana

Paralogous to G1792

1355
G43
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G46

1356
G43
PRT

Arabidopsis thaliana

Paralogous to G46

1357
G131
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G155

1358
G131
PRT

Arabidopsis thaliana

Paralogous to G155

1359
G135
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G155

1360
G135
PRT

Arabidopsis thaliana

Paralogous to G155

1361
G142
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G148

1362
G142
PRT

Arabidopsis thaliana

Paralogous to G148

1363
G149
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G627

1364
G149
PRT

Arabidopsis thaliana

Paralogous to G627

1365
G152
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G153

1366
G152
PRT

Arabidopsis thaliana

Paralogous to G153

1367
G154
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1011

1368
G154
PRT

Arabidopsis thaliana

Paralogous to G1011

1369
G184
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G916

1370
G184
PRT

Arabidopsis thaliana

Paralogous to G916

1371
G186
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G916

1372
G186
PRT

Arabidopsis thaliana

Paralogous to G916

1373
G204
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2717

1374
G204
PRT

Arabidopsis thaliana

Paralogous to G2717

1375
G225
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1816, G2718

1376
G225
PRT

Arabidopsis thaliana

Paralogous to G1816, G2718

1377
G226
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1816, G2718

1378
G226
PRT

Arabidopsis thaliana

Paralogous to G1816, G2718

1379
G353
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2839

1380
G353
PRT

Arabidopsis thaliana

Paralogous to G2839

1381
G354
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2839

1382
G354
PRT

Arabidopsis thaliana

Paralogous to G2839

1383
G361
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G370, G1995, G2826,





G2838

1384
G361
PRT

Arabidopsis thaliana

Paralogous to G370, G1995, G2826, G2838

1385
G362
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G370, G1995, G2826,





G2838

1386
G362
PRT

Arabidopsis thaliana

Paralogous to G370, G1995, G2826, G2838

1387
G370
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1995, G2826, G2838

1388
G370
PRT

Arabidopsis thaliana

Paralogous to G1995, G2826, G2838

1389
G390
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G438

1390
G390
PRT

Arabidopsis thaliana

Paralogous to G438

1391
G391
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G438

1392
G391
PRT

Arabidopsis thaliana

Paralogous to G438

1393
G392
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G438

1394
G392
PRT

Arabidopsis thaliana

Paralogous to G438

1395
G481
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G485

1396
G481
PRT

Arabidopsis thaliana

Paralogous to G485

1397
G482
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G485

1398
G482
PRT

Arabidopsis thaliana

Paralogous to G485

1399
G511
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2536

1400
G511
PRT

Arabidopsis thaliana

Paralogous to G2536

1401
G512
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1357, G1452

1402
G512
PRT

Arabidopsis thaliana

Paralogous to G1357, G1452

1403
G600
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2768

1404
G600
PRT

Arabidopsis thaliana

Paralogous to G2768

1405
G617
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2650

1406
G617
PRT

Arabidopsis thaliana

Paralogous to G2650

1407
G682
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1816, G2718

1408
G682
PRT

Arabidopsis thaliana

Paralogous to G1816, G2718

1409
G722
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1037

1410
G722
PRT

Arabidopsis thaliana

Paralogous to G1037

1411
G736
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2432

1412
G736
PRT

Arabidopsis thaliana

Paralogous to G2432

1413
G759
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1412

1414
G759
PRT

Arabidopsis thaliana

Paralogous to G1412

1415
G773
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1412

1416
G773
PRT

Arabidopsis thaliana

Paralogous to G1412

1417
G810
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G807

1418
G810
PRT

Arabidopsis thaliana

Paralogous to G807

1419
G860
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G153

1420
G860
PRT

Arabidopsis thaliana

Paralogous to G153

1421
G957
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G961

1422
G957
PRT

Arabidopsis thaliana

Paralogous to G961

1423
G990
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1451

1424
G990
PRT

Arabidopsis thaliana

Paralogous to G1451

1425
G1004
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G46

1426
G1004
PRT

Arabidopsis thaliana

Paralogous to G46

1427
G1017
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2567

1428
G1017
PRT

Arabidopsis thaliana

Paralogous to G2567

1429
G1196
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G839

1430
G1196
PRT

Arabidopsis thaliana

Paralogous to G839

1431
G1207
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1206

1432
G1207
PRT

Arabidopsis thaliana

Paralogous to G1206

1433
G1325
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1313

1434
G1325
PRT

Arabidopsis thaliana

Paralogous to G1313

1435
G1335
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G652

1436
G1335
PRT

Arabidopsis thaliana

Paralogous to G652

1437
G1357
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1452

1438
G1357
PRT

Arabidopsis thaliana

Paralogous to G1452

1439
G1364
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G485

1440
G1364
PRT

Arabidopsis thaliana

Paralogous to G485

1441
G1379
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G12

1442
G1379
PRT

Arabidopsis thaliana

Paralogous to G12

1443
G1387
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G975

1444
G1387
PRT

Arabidopsis thaliana

Paralogous to G975

1445
G1399
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1128

1446
G1399
PRT

Arabidopsis thaliana

Paralogous to G1128

1447
G1419
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G46

1448
G1419
PRT

Arabidopsis thaliana

Paralogous to G46

1449
G1435
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2741

1450
G1435
PRT

Arabidopsis thaliana

Paralogous to G2741

1451
G1452
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1357

1452
G1452
PRT

Arabidopsis thaliana

Paralogous to G1357

1453
G1548
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G438

1454
G1548
PRT

Arabidopsis thaliana

Paralogous to G438

1455
G1652
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2776

1456
G1652
PRT

Arabidopsis thaliana

Paralogous to G2776

1457
G1749
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1840

1458
G1749
PRT

Arabidopsis thaliana

Paralogous to G1840

1459
G1760
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G153

1460
G1760
PRT

Arabidopsis thaliana

Paralogous to G153

1461
G1791
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G30, G1792

1462
G1791
PRT

Arabidopsis thaliana

Paralogous to G30, G1792

1463
G1792
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G30

1464
G1792
PRT

Arabidopsis thaliana

Paralogous to G30

1465
G1795
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G30, G1792

1466
G1795
PRT

Arabidopsis thaliana

Paralogous to G30, G1792

1467
G1797
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1798

1468
G1797
PRT

Arabidopsis thaliana

Paralogous to G1798

1469
G1798
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1797

1470
G1798
PRT

Arabidopsis thaliana

Paralogous to G1797

1471
G1816
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2718

1472
G1816
PRT

Arabidopsis thaliana

Paralogous to G2718

1473
G1839
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1840

1474
G1839
PRT

Arabidopsis thaliana

Paralogous to G1840

1475
G1863
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2334

1476
G1863
PRT

Arabidopsis thaliana

Paralogous to G2334

1477
G1888
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1482

1478
G1888
PRT

Arabidopsis thaliana

Paralogous to G1482

1479
G1889
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2839

1480
G1889
PRT

Arabidopsis thaliana

Paralogous to G2839

1481
G1914
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G651

1482
G1914
PRT

Arabidopsis thaliana

Paralogous to G651

1483
G1940
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2854

1484
G1940
PRT

Arabidopsis thaliana

Paralogous to G2854

1485
G1973
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G651

1486
G1973
PRT

Arabidopsis thaliana

Paralogous to G651

1487
G1974
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2839

1488
G1974
PRT

Arabidopsis thaliana

Paralogous to G2839

1489
G1976
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1893

1490
G1976
PRT

Arabidopsis thaliana

Paralogous to G1893

1491
G1994
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G355

1492
G1994
PRT

Arabidopsis thaliana

Paralogous to G355

1493
G1995
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G370, G2826, G2838

1494
G1995
PRT

Arabidopsis thaliana

Paralogous to G370, G2826, G2838

1495
G2133
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G47

1496
G2133
PRT

Arabidopsis thaliana

Paralogous to G47

1497
G2199
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is arab ous to G2207

1498
G2199
PRT

Arabidopsis thaliana

Paralogous to G2207

1499
G2334
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1863

1500
G2334
PRT

Arabidopsis thaliana

Paralogous to G1863

1501
G2345
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G485

1502
G2345
PRT

Arabidopsis thaliana

Paralogous to G485

1503
G2457
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2459

1504
G2457
PRT

Arabidopsis thaliana

Paralogous to G2459

1505
G2459
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2457

1506
G2459
PRT

Arabidopsis thaliana

Paralogous to G2457

1507
G2535
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G961

1508
G2535
PRT

Arabidopsis thaliana

Paralogous to G961

1509
G2546
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2550

1510
G2546
PRT

Arabidopsis thaliana

Paralogous to G2550

1511
G2562
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2830

1512
G2562
PRT

Arabidopsis thaliana

Paralogous to G2830

1513
G2563
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2830

1514
G2563
PRT

Arabidopsis thaliana

Paralogous to G2830

1515
G2583
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G975

1516
G2583
PRT

Arabidopsis thaliana

Paralogous to G975

1517
G2632
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G926

1518
G2632
PRT

Arabidopsis thaliana

Paralogous to G926

1519
G2635
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2505

1520
G2635
PRT

Arabidopsis thaliana

Paralogous to G2505

1521
G2659
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1142

1522
G2659
PRT

Arabidopsis thaliana

Paralogous to G1142

1523
G2664
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1928

1524
G2664
PRT

Arabidopsis thaliana

Paralogous to G1928

1525
G2709
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2717

1526
G2709
PRT

Arabidopsis thaliana

Paralogous to G2717

1527
G2718
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1816

1528
G2718
PRT

Arabidopsis thaliana

Paralogous to G1816

1529
G2779
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2859

1530
G2779
PRT

Arabidopsis thaliana

Paralogous to G2859

1531
G2826
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G370, G1995, G2838

1532
G2826
PRT

Arabidopsis thaliana

Paralogous to G370, G1995, G2838

1533
G2828
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2830

1534
G2828
PRT

Arabidopsis thaliana

Paralogous to G2830

1535
G2838
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G370, G1995, G2826

1536
G2838
PRT

Arabidopsis thaliana

Paralogous to G370, G1995, G2826

1537
G2882
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2041

1538
G2882
PRT

Arabidopsis thaliana

Paralogous to G2041

1539
G2928
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2933

1540
G2928
PRT

Arabidopsis thaliana

Paralogous to G2933

1541
G2932
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2933

1542
G2932
PRT

Arabidopsis thaliana

Paralogous to G2933

1543
G2934
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2865

1544
G2934
PRT

Arabidopsis thaliana

Paralogous to G2865

1545
G2966
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G3067

1546
G2966
PRT

Arabidopsis thaliana

Paralogous to G3067

1547
G2980
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2979

1548
G2980
PRT

Arabidopsis thaliana

Paralogous to G2979

1549
G2981
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2982

1550
G2981
PRT

Arabidopsis thaliana

Paralogous to G2982

1551
G2982
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2981

1552
G2982
PRT

Arabidopsis thaliana

Paralogous to G2981

1553
G2989
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G2990

1554
G2989
PRT

Arabidopsis thaliana

Paralogous to G2990

1555
G3062
DNA

Arabidopsis thaliana

Predicted polypeptide sequence is paralogous to G1893

1556
G3062
PRT

Arabidopsis thaliana

Paralogous to G1893

1557

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G12

1558

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G12

1559

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G30

1560

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G148

1561

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G148

1562

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G148

1563

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G155

1564

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G155

1565

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G155

1566

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G155

1567

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G200

1568

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G319

1569

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G355

1570

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G370

1571

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

1572

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

1573

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

1574

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G438

1575

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G627

1576

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G651

1577

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G652

1578

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G652

1579

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G652

1580

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G807

1581

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1011

1582

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1313

1583

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1313

1584

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1451

1585

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1451

1586

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1451

1587

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1468

1588

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1468

1589

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1468

1590

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1468

1591

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1468

1592

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1482

1593

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1660

1594

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1796

1595

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1596

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1597

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1598

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1599

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1600

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1983

1601

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1988

1602

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2060

1603

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2109

1604

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2109

1605

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2207

1606

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2207

1607

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2317

1608

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2432

1609

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2453

1610

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2550

1611

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2567

1612

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2567

1613

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2650

1614

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2717

1615

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2754

1616

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2826

1617

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2839

1618

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2854

1619

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2854

1620

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2907

1621

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2907

1622

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2907

1623

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2913

1624

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2933

1625

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2969

1626

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2981

1627

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2983

1628

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2990

1629

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2992

1630

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G3076

1631

DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G3083

1632

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G12

1633

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G30, G1792

1634

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G153

1635

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G155

1636

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G355

1637

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G370

1638

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

1639

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

1640

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G438

1641

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485

1642

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G624

1643

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

1644

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

1645

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G652

1646

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G839

1647

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G839

1648

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

1649

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G916

1650

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G961

1651

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1011

1652

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1037

1653

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1128

1654

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

1655

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

1656

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1206

1657

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1412

1658

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1451

1659

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1451

1660

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1468

1661

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1468

1662

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1510

1663

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1816, G2718

1664

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1893

1665

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1928

1666

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2142

1667

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2207

1668

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2317

1669

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2567

1670

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2717

1671

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2741

1672

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1673

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2776

1674

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2784

1675

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2839

1676

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2839

1677

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2854

1678

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2865

1679

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2907

1680

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2969

1681

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2969

1682

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2969

1683

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2981

1684

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2983

1685

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G2990

1686

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3055

1687

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3083

1688

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3086

1689

DNA

Glycine max

Predicted polypeptide sequence is orthologous to G3086

1690

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G12

1691

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G200

1692

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G355

1693

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G438

1694

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G438

1695

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G627

1696

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1011

1697

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1206

1698

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1206

1699

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1276

1700

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1451

1701

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1468

1702

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1468

1703

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1482

1704

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G1928

1705

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2051

1706

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2060

1707

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2085

1708

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2142

1709

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2317

1710

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2382

1711

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2550

1712

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2550

1713

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2839

1714

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2983

1715

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G2990

1716

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G3067

1717

DNA

Medicago truncatula

Predicted polypeptide sequence is orthologous to G3067

1718

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G47

1719

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G155

1720

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G155

1721

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G155

1722

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G438

1723

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G438

1724

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G438

1725

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G485

1726

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G485

1727

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G627

1728

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G652

1729

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G652

1730

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G652

1731

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G807

1732

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G916

1733

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G975

1734

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G1313

1735

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G1451

1736

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G1468

1737

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G1468

1738

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G1468

1739

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G1928

1740

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2051

1741

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2317

1742

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2457

1743

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2567

1744

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2717

1745

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2741

1746

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2754

1747

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2763

1748

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2768

1749

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2839

1750

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2854

1751

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2854

1752

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G2907

1753

DNA

Hordeum vulgare

Predicted polypeptide sequence is orthologous to G3083

1754

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G30, G1792

1755

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

1756

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G148

1757

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153

1758

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153

1759

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

1760

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

1761

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

1762

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

1763

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

1764

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G155

1765

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

1766

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

1767

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

1768

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

1769

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G200

1770

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G319

1771

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

1772

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

1773

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

1774

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

1775

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G438

1776

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

1777

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

1778

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485

1779

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G651

1780

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G651

1781

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G651

1782

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G652

1783

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G807

1784

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G839

1785

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G916

1786

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

1787

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1011

1788

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1037

1789

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1037

1790

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1142

1791

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1206

1792

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1793

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1794

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1795

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1796

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1797

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1451

1798

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1468

1799

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1468

1800

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1468

1801

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1468

1802

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1482

1803

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1804

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1660

1805

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1796

1806

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1893

1807

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1983

1808

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1983

1809

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1983

1810

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2041

1811

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2109

1812

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2142

1813

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2453

1814

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2457

1815

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2550

1816

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2567

1817

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2579

1818

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2717

1819

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2741

1820

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2754

1821

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2754

1822

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2754

1823

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2854

1824

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2854

1825

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2907

1826

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2907

1827

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2930

1828

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2933

1829

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2981

1830

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2981

1831

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G2992

1832

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3055

1833

DNA

Zea mays

Predicted polypeptide sequence is orthologous to G3067

1834

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G30

1835

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G148

1836

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G155

1837

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G155

1838

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G155

1839

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G319

1840

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G438

1841

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G438

1842

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G438

1843

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G438

1844

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G438

1845

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G438

1846

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G485

1847

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G485

1848

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G485

1849

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G485

1850

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G627

1851

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G652

1852

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G652

1853

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G652

1854

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G652

1855

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G652

1856

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G839

1857

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G926

1858

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1011

1859

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1011

1860

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1011

1861

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1037

1862

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1037

1863

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1128

1864

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1451

1865

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1451

1866

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1451

1867

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1868

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1869

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1870

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1871

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1872

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1873

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1874

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1875

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1876

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1877

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1878

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1468

1879

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1482

1880

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1510

1881

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1660

1882

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1796

1883

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1816, G2718

1884

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1840

1885

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1886

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1887

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1888

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1889

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1890

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1891

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1892

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1893

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1894

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1895

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1896

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G1983

1897

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2060

1898

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2109

1899

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2142

1900

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2142

1901

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2142

1902

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2382

1903

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2457, G2459

1904

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2457

1905

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2550

1906

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2567

1907

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2717

1908

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2717

1909

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2717

1910

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2754

1911

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2754

1912

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2768

1913

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2776

1914

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2839

1915

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2839

1916

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2839

1917

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2839

1918

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2854

1919

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2854

1920

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2854

1921

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G2990

1922

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G3055

1923

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G3070

1924

DNA

Triticum aestivum

Predicted polypeptide sequence is orthologous to G3083

1925

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G46

1926

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1927

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1928

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G652

1929

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1011

1930

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1412

1931

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

1932

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1510

1933

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2317

1934

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2907

1935

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2930

1936

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2969

1937

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G12

1938

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G12

1939

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G12

1940

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G46

1941

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G46

1942

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G46

1943

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G148

1944

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G148

1945

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G153

1946

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G153

1947

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1948

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1949

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1950

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1951

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1952

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G155

1953

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G200

1954

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G200

1955

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G319

1956

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G355

1957

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G355

1958

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G355

1959

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

1960

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

1961

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G370, G1995, G2826,





G2838

1962

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1963

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1964

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1965

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1966

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1967

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1968

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1969

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1970

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1971

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1972

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1973

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1974

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1975

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1976

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1977

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1978

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1979

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G438

1980

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G485

1981

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G485

1982

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G627

1983

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G651

1984

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G651

1985

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G651

1986

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G652

1987

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G652

1988

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G807

1989

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G807

1990

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G839

1991

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G839

1992

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G839

1993

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G916

1994

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G916

1995

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G916

1996

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G916

1997

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G916

1998

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G916

1999

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G926

2000

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G926

2001

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G926

2002

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G961

2003

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G975

2004

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G975

2005

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G975

2006

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G975

2007

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1011

2008

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1011

2009

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1011

2010

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1011

2011

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1037

2012

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1142

2013

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1206

2014

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1206

2015

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1206

2016

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1206

2017

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1274

2018

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1274

2019

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1276

2020

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1357, G1452

2021

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1412

2022

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1412

2023

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2024

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2025

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2026

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2027

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2028

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2029

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2030

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1451

2031

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1468

2032

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1482

2033

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1510

2034

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1660

2035

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1660

2036

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1779

2037

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1893

2038

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1928

2039

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1928

2040

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1928

2041

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1928

2042

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1928

2043

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1968

2044

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1983

2045

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G1988

2046

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2041

2047

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2041

2048

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2142

2049

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2142

2050

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2142

2051

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2142

2052

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2207

2053

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2207

2054

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2319

2055

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2334

2056

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2382

2057

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2382

2058

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2432

2059

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2459

2060

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2536

2061

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2536

2062

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2567

2063

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2567

2064

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2567

2065

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2571

2066

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2650

2067

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2650

2068

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2717

2069

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2717

2070

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2741

2071

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2741

2072

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2768

2073

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2768

2074

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2768

2075

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2776

2076

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2776

2077

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2784

2078

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2839

2079

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2854

2080

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2854

2081

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2854

2082

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2854

2083

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2854

2084

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2854

2085

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2859

2086

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2865

2087

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2865

2088

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2885

2089

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2907

2090

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2933

2091

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2969

2092

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2979

2093

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2981, G2982

2094

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2983

2095

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2990

2096

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G2990

2097

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G3055

2098

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G3067

2099

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G3067

2100

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G3076

2101

DNA

Lycopersicon esculentum

Predicted polypeptide sequence is orthologous to G3083

2102
G131_1
DNA

Arabidopsis thaliana

Expression construct P15154 (sequence variant)

2103
G324_1
DNA

Arabidopsis thaliana

Expression construct P3299 (sequence variant)

2104
G386_1
DNA

Arabidopsis thaliana

Expression construct P15647 (sequence variant)

2105
G624_1
DNA

Arabidopsis thaliana

Expression construct P2461.7 (sequence variant)

2106
G651_1
DNA

Arabidopsis thaliana

Expression construct P2812 (sequence variant)

2107
G744_1
DNA

Arabidopsis thaliana

Expression construct P15010 (sequence variant)

2108
G1037_1
DNA

Arabidopsis thaliana

Expression construct P15001 (sequence variant)

2109
G1150_1
DNA

Arabidopsis thaliana

Expression construct P15631 (sequence variant)

2110
G2041_1
DNA

Arabidopsis thaliana

Expression construct P13846 (sequence variant)

2111
G2106_1
DNA

Arabidopsis thaliana

Expression construct P13733 (sequence variant)

2112
G2319_1
DNA

Arabidopsis thaliana

Expression construct P13388 (sequence variant)

2113
G2453_1
DNA

Arabidopsis thaliana

Expression construct P2750 (sequence variant)

2114
G2453_2
DNA

Arabidopsis thaliana

Expression construct P3322 (sequence variant)

2115
G2559_1
DNA

Arabidopsis thaliana

Expression construct P15538 (sequence variant)

2116
G2639_1
DNA

Arabidopsis thaliana

Expression construct P15568 (sequence variant)

2117
G2679_1
DNA

Arabidopsis thaliana

Expression construct P15056 (sequence variant)

2118
G2768_1
DNA

Arabidopsis thaliana

Expression construct P15431 (sequence variant)

2119
G2771_1
DNA

Arabidopsis thaliana

Expression construct P15182 (sequence variant)

2120
G2784_1
DNA

Arabidopsis thaliana

Expression construct P15148 (sequence variant)

2121
G2802_1
DNA

Arabidopsis thaliana

Expression construct P2771 (sequence variant)

2122
G2907_1
DNA

Arabidopsis thaliana

Expression construct P15595 (sequence variant)

2123
G3003_1
DNA

Arabidopsis thaliana

Expression construct P3291 (sequence variant)

2124
G3380
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1795





Member of G1792 clade

2125
G3380
PRT

Oryza sativa

Orthologous to G1795 Member of G1792 clade

2126
G3381
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G30





Member of G1792 clade

2127
G3381
PRT

Oryza sativa

Orthologous to G30 Member of G1792 clade

2128
G3383
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G1792





Member of G1792 clade

2129
G3383
PRT

Oryza sativa

Orthologous to G1792 Member of G1792 clade

2130
G3392
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G682





Member of G1816 and G2718 clade

2131
G3392
PRT

Oryza sativa

Orthologous to G682 Member of G1816 and G2718 clade

2132
G3393
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G682





Member of G1816 and G2718 clade

2133
G3393
PRT

Oryza sativa

Orthologous to G682 Member of G1816 and G2718 clade

2134
G3394
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2135
G3394
PRT

Oryza sativa

Orthologous to G485 Member of G485 clade

2136
G3395
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2137
G3395
PRT

Oryza sativa

Orthologous to G485 Member of G485 clade

2138
G3396
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2139
G3396
PRT

Oryza sativa

Orthologous to G485 Member of G485 clade

2140
G3397
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2141
G3397
PRT

Oryza sativa

Orthologous to G485 Member of G485 clade

2142
G3398
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2143
G3398
PRT

Oryza sativa

Orthologous to G485 Member of G485 clade

2144
G3429
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2145
G3429
PRT

Oryza sativa

Orthologous to G485 Member of G485 clade

2146
G3431
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G682





Member of G1816 and G2718 clade

2147
G3431
PRT

Zea mays

Orthologous to G682 Member of G1816 and G2718 clade

2148
G3434
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2149
G3434
PRT

Zea mays

Orthologous to G485 Member of G485 clade

2150
G3435
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G482





Member of G485 clade

2151
G3435
PRT

Zea mays

Orthologous to G482 Member of G485 clade

2152
G3436
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2153
G3436
PRT

Zea mays

Orthologous to G485 Member of G485 clade

2154
G3437
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2155
G3437
PRT

Zea mays

Orthologous to G485 Member of G485 clade

2156
G3444
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G682





Member of G1816 and G2718 clade

2157
G3444
PRT

Zea mays

Orthologous to G682 Member of G1816 and G2718 clade

2158
G3445
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G225





Member of G1816 and G2718 clade

2159
G3445
PRT

Glycine max

Orthologous to G225 Member of G1816 and G2718 clade

2160
G3446
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G225





Member of G1816 and G2718 clade

2161
G3446
PRT

Glycine max

Orthologous to G225 Member of G1816 and G2718 clade

2162
G3447
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G225





Member of G1816 and G2718 clade

2163
G3447
PRT

Glycine max

Orthologous to G225 Member of G1816 and G2718 clade

2164
G3448
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G225





Member of G1816 and G2718 clade

2165
G3448
PRT

Glycine max

Orthologous to G225 Member of G1816 and G2718 clade

2166
G3449
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G225





Member of G1816 and G2718 clade

2167
G3449
PRT

Glycine max

Orthologous to G225 Member of G1816 and G2718 clade

2168
G3450
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G682





Member of G1816 and G2718 clade

2169
G3450
PRT

Glycine max

Orthologous to G682 Member of G1816 and G2718 clade

2170
G3470
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G482





Member of G485 clade

2171
G3470
PRT

Glycine max

Orthologous to G482 Member of G485 clade

2172
G3471
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G482





Member of G485 clade

2173
G3471
PRT

Glycine max

Orthologous to G482 Member of G485 clade

2174
G3472
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2175
G3472
PRT

Glycine max

Orthologous to G485 Member of G485 clade

2176
G3473
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2177
G3473
PRT

Glycine max

Orthologous to G485 Member of G485 clade

2178
G3474
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2179
G3474
PRT

Glycine max

Orthologous to G485 Member of G485 clade

2180
G3475
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2181
G3475
PRT

Glycine max

Orthologous to G485 Member of G485 clade

2182
G3476
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G482





Member of G485 clade

2183
G3476
PRT

Glycine max

Orthologous to G485 Member of G482 clade

2184
G3477
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G482





Member of G485 clade

2185
G3477
PRT

Glycine max

Orthologous to G485 Member of G482 clade

2186
G3478
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G485





Member of G485 clade

2187
G3478
PRT

Glycine max

Orthologous to G485 Member of G485 clade

2188
G3479
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G153





Member of G153 clade

2189
G3479
PRT

Oryza sativa

Orthologous to G153 Member of G153 clade

2190
G3484
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G153





Member of G153 clade

2191
G3484
PRT

Glycine max

Orthologous to G153 Member of G153 clade

2192
G3485
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G153





Member of G153 clade

2193
G3485
PRT

Glycine max

Orthologous to G153 Member of G153 clade

2194
G3487
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153





Member of G153 clade

2195
G3487
PRT

Zea mays

Orthologous to G153 Memberof G153 clade

2196
G3488
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153





Member of G153 clade

2197
G3488
PRT

Zea mays

Orthologous to G153 Member of G153 clade

2198
G3489
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G153





Member of G153 clade

2199
G3489
PRT

Zea mays

Orthologous to G153 Member of G153 clade

2200
G3491
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G807





Member of G807 clade

2201
G3491
PRT

Oryza sativa

Orthologous to G807 Member of G807 clade

2202
G3494
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G807





Member of G807 clade

2203
G3494
PRT

Glycine max

Orthologous to G807 Member of G807 clade

2204
G3495
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G807





Member of G807 clade

2205
G3495
PRT

Glycine max

Orthologous to G807 Member of G807 clade

2206
G3512
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G807





Member of G807 clade

2207
G3512
PRT

Glycine max

Orthologous to G807 Member of G807 clade

2208
G3515
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G30





Member of G1792 clade

2209
G3515
PRT

Oryza sativa

Orthologous to G30 Member of G1792 clade

2210
G3516
DNA

Zea mays

Predicted polypeptide sequence is orthologous to G1792





Member of G1792 clade

2211
G3516
PRT

Zea mays

Orthologous to G1792 Member of G1792 clade

2212
G3517
DNA

Zea mays

Predicted polypeptide sequence is orthologous to 01791





Member of G1792 clade

2213
G3517
PRT

Zea mays

Orthologous to G1791 Member of G1792 clade

2214
G3518
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1792





Member of G1792 clade

2215
G3518
PRT

Glycine max

Orthologous to G1792 Member of G1792 clade

2216
G3519
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1792





Member of G1792 clade

2217
G3519
PRT

Glycine max

Orthologous to G1792 Member of G1792 clade

2218
G3520
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G1792





Member of G1792 clade

2219
G3520
PRT

Glycine max

Orthologous to G1792 Member of G1792 clade

2220
G3527
DNA

Glycine max


2221
G3527
PRT

Glycine max


2222
G3528
DNA

Glycine max


2223
G3528
PRT

Glycine max


2224
G3643
DNA

Glycine max

Predicted polypeptide sequence is orthologous to G47





Member of G47 and G2133 clade

2225
G3643
PRT

Glycine max

Orthologous to G47 Member of G47 and G2133 clade

2226
G3644
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G47





Member of G47 and G2133 clade

2227
G3644
PRT

Oryza sativa

Orthologous to G47 Member of G47 and G2133 clade

2228
G3645
DNA

Brassica rapa

Predicted polypeptide sequence is orthologous to G47





Member of G47 and G2133 clade

2229
G3645
PRT

Brassica rapa

Orthologous to G47 Member of G47 and G2133 clade

2230
G3646
DNA

Brassica oleracea

Predicted polypeptide sequence is orthologous to G2133





Member of G47 and G2133 clade

2231
G3646
PRT

Brassica oleracea

Orthologous to G2133 Member of G47 and G2133 clade

2232
G3647
DNA

Zinnia elegans

Predicted polypeptide sequence is orthologous to G47





Member of G47 and G2133 clade

2233
G3647
PRT

Zinnia elegans

Orthologous to G47 Member of G47 and G2133 clade

2234
G3649
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G47 and G2133





Member of G47 and G2133 clade

2235
G3649
PRT

Oryza sativa

Orthologous to G47 and G2133 Member of G47 and G2133 clade

2236
G3651
DNA

Oryza sativa

Predicted polypeptide sequence is orthologous to G2133





Member of G47 and G2133 clade

2237
G3651
PRT

Oryza sativa

Orthologous to G2133 Member of G47 and G2133 clade



























































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































































EXAMPLES
The invention, now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present invention and are not intended to limit the invention. It will be recognized by one of skill in the art that a transcription factor that is associated with a particular first trait may also be associated with at least one other, unrelated and inherent second trait which was not predicted by the first trait.
The complete descriptions of the traits associated with each polynucleotide of the invention are fully disclosed in Table 4 and Table 6. The complete description of the transcription factor gene family and identified conserved domains of the polypeptide encoded by the polynucleotide is fully disclosed in Table 5.
Example I
Full Length Gene Identification and Cloning
Putative transcription factor sequences (genomic or ESTs) related to known transcription factors were identified in the Arabidopsis thaliana GenBank database using the tblastn sequence analysis program using default parameters and a P-value cutoff threshold of −4 or −5 or lower, depending on the length of the query sequence. Putative transcription factor sequence hits were then screened to identify those containing particular sequence strings. If the sequence hits contained such sequence strings, the sequences were confirmed as transcription factors.
Alternatively, Arabidopsis thaliana cDNA libraries derived from different tissues or treatments, or genomic libraries were screened to identify novel members of a transcription family using a low stringency hybridization approach. Probes were synthesized using gene specific primers in a standard PCR reaction (annealing temperature 60° C.) and labeled with 32 P dCTP using the High Prime DNA Labeling Kit (Boehringer Mannheim Corp. (now Roche Diagnostics Corp., Indianapolis, Ind.). Purified radiolabelled probes were added to filters immersed in Church hybridization medium (0.5 M NaPO 4 pH 7.0, 7% SDS, 1% w/v bovine serum albumin) and hybridized overnight at 60° C. with shaking. Filters were washed two times for 45 to 60 minutes with 1×SCC, 1% SDS at 60° C.
To identify additional sequence 5′ or 3′ of a partial cDNA sequence in a cDNA library, 5′ and 3′ rapid amplification of cDNA ends (RACE) was performed using the MARATHON cDNA amplification kit (Clontech, Palo Alto, Calif.). Generally, the method entailed first isolating poly(A) mRNA, performing first and second strand cDNA synthesis to generate double stranded cDNA, blunting cDNA ends, followed by ligation of the MARATHON Adaptor to the cDNA to form a library of adaptor-ligated ds cDNA.
Gene-specific primers were designed to be used along with adaptor specific primers for both 5′ and 3′ RACE reactions. Nested primers, rather than single primers, were used to increase PCR specificity. Using 5′ and 3′ RACE reactions, 5′ and 3′ RACE fragments were obtained, sequenced and cloned. The process can be repeated until 5′ and 3′ ends of the full-length gene were identified. Then the full-length cDNA was generated by PCR using primers specific to 5′ and 3′ ends of the gene by end-to-end PCR.
Example II
Construction of Expression Vectors
The sequence was amplified from a genomic or cDNA library using primers specific to sequences upstream and downstream of the coding region. The expression vector was pMEN20 or pMEN65, which are both derived from pMON316 (Sanders et al. (1987) Nucleic Acids Res. 15:1543–1558) and contain the CaMV 35S promoter to express transgenes. To clone the sequence into the vector, both pMEN20 and the amplified DNA fragment were digested separately with SalI and NotI restriction enzymes at 37° C. for 2 hours. The digestion products were subject to electrophoresis in a 0.8% agarose gel and visualized by ethidium bromide staining. The DNA fragments containing the sequence and the linearized plasmid were excised and purified by using a QIAQUICK gel extraction kit (Qiagen, Valencia Calif.). The fragments of interest were ligated at a ratio of 3:1 (vector to insert). Ligation reactions using T4 DNA ligase (New England Biolabs, Beverly Mass.) were carried out at 16° C. for 16 hours. The ligated DNAs were transformed into competent cells of the E. coli strain DH5 alpha by using the heat shock method. The transformations were plated on LB plates containing 50 mg/l kanamycin (Sigma Chemical Co. St. Louis Mo.). Individual colonies were grown overnight in five milliliters of LB broth containing 50 mg/l kanamycin at 37° C. Plasmid DNA was purified by using Qiaquick Mini Prep kits (Qiagen).
Example III
Transformation of Agrobacterium with the Expression Vector
After the plasmid vector containing the gene was constructed, the vector was used to transform Agrobacterium tumefaciens cells expressing the gene products. The stock of Agrobacterium tumefaciens cells for transformation was made as described by Nagel et al. (1990) FEMS Microbiol Letts. 67: 325–328. Agrobacterium strain ABI was grown in 250 ml LB medium (Sigma) overnight at 28° C. with shaking until an absorbance over 1 cm at 600 nm (A 600 ) of 0.5–1.0 was reached. Cells were harvested by centrifugation at 4,000×g for 15 min at 4° C. Cells were then resuspended in 250 μl chilled buffer (1 mM HEPES, pH adjusted to 7.0 with KOH). Cells were centrifuged again as described above and resuspended in 125 μl chilled buffer. Cells were then centrifuged and resuspended two more times in the same HEPES buffer as described above at a volume of 100 μl and 750 μl, respectively. Resuspended cells were then distributed into 40 μl aliquots, quickly frozen in liquid nitrogen, and stored at −80° C.
Agrobacterium cells were transformed with plasmids prepared as described above following the protocol described by Nagel et al. (supra). For each DNA construct to be transformed, 50–100 ng DNA (generally resuspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0) was mixed with 40 μl of Agrobacterium cells. The DNA/cell mixture was then transferred to a chilled cuvette with a 2 mm electrode gap and subject to a 2.5 kV charge dissipated at 25 μF and 200 μF using a Gene Pulser II apparatus (Bio-Rad, Hercules, Calif.). After electroporation, cells were immediately resuspended in 1.0 ml LB and allowed to recover without antibiotic selection for 2–4 hours at 28° C. in a shaking incubator. After recovery, cells were plated onto selective medium of LB broth containing 100 μg/ml spectinomycin (Sigma) and incubated for 24–48 hours at 28° C. Single colonies were then picked and inoculated in fresh medium. The presence of the plasmid construct was verified by PCR amplification and sequence analysis.
Example IV
Transformation of Arabidopsis Plants with Agrobacterium tumefaciens with Expression Vector
After transformation of Agrobacterium tumefaciens with plasmid vectors containing the gene, single Agrobacterium colonies were identified, propagated, and used to transform Arabidopsis plants. Briefly, 500 ml cultures of LB medium containing 50 mg/l kanamycin were inoculated with the colonies and grown at 28° C. with shaking for 2 days until an optical absorbance at 600 nm wavelength over 1 cm (A 600 ) of >2.0 is reached. Cells were then harvested by centrifugation at 4,000×g for 10 min, and resuspended in infiltration medium (½× Murashige and Skoog salts (Sigma), 1× Gamborg's B-5 vitamins (Sigma), 5.0% (w/v) sucrose (Sigma), 0.044 μM benzylamino purine (Sigma), 200 μl/l Silwet L-77 (Lehle Seeds) until an A 600 of 0.8 was reached.
Prior to transformation, Arabidopsis thaliana seeds (ecotype Columbia) were sown at a density of ˜10 plants per 4″ pot onto Pro-Mix BX potting medium (Hummert International) covered with fiberglass mesh (18 mm×16 mm). Plants were grown under continuous illumination (50–75 μE/m 2 /sec) at 22–23° C. with 65–70% relative humidity. After about 4 weeks, primary inflorescence stems (bolts) are cut off to encourage growth of multiple secondary bolts. After flowering of the mature secondary bolts, plants were prepared for transformation by removal of all siliques and opened flowers.
The pots were then immersed upside down in the mixture of Agrobacterium infiltration medium as described above for 30 sec, and placed on their sides to allow draining into a 1′×2′ flat surface covered with plastic wrap. After 24 h, the plastic wrap was removed and pots are turned upright. The immersion procedure was repeated one week later, for a total of two immersions per pot. Seeds were then collected from each transformation pot and analyzed following the protocol described below.
Example V
Identification of Arabidopsis Primary Transformants
Seeds collected from the transformation pots were sterilized essentially as follows. Seeds were dispersed into in a solution containing 0.1% (v/v) Triton X-100 (Sigma) and sterile water and washed by shaking the suspension for 20 min. The wash solution was then drained and replaced with fresh wash solution to wash the seeds for 20 min with shaking. After removal of the ethanol/detergent solution, a solution containing 0.1% (v/v) Triton X-100 and 30% (v/v) bleach (CLOROX; Clorox Corp. Oakland Calif.) was added to the seeds, and the suspension was shaken for 10 min. After removal of the bleach/detergent solution, seeds were then washed five times in sterile distilled water. The seeds were stored in the last wash water at 4° C. for 2 days in the dark before being plated onto antibiotic selection medium (1× Murashige and Skoog salts (pH adjusted to 5.7 with 1M KOH), 1× Gamborg's B-5 vitamins, 0.9% phytagar (Life Technologies), and 50 mg/l kanamycin). Seeds were germinated under continuous illumination (50–75 μE/m 2 /sec) at 22–23° C. After 7–10 days of growth under these conditions, kanamycin resistant primary transformants (T1 generation) were visible and obtained. These seedlings were transferred first to fresh selection plates where the seedlings continued to grow for 3–5 more days, and then to soil (Pro-Mix BX potting medium).
Primary transformants were crossed and progeny seeds (T 2 ) collected; kanamycin resistant seedlings were selected and analyzed. The expression levels of the recombinant polynucleotides in the transformants varies from about a 5% expression level increase to a least a 100% expression level increase. Similar observations are made with respect to polypeptide level expression.
Example VI
Identification of Arabidopsis Plants with Transcription Factor Gene Knockouts
The screening of insertion mutagenized Arabidopsis collections for null mutants in a known target gene was essentially as described in Krysan et al. (1999) Plant Cell 11: 2283–2290. Briefly, gene-specific primers, nested by 5–250 base pairs to each other, were designed from the 5′ and 3′ regions of a known target gene. Similarly, nested sets of primers were also created specific to each of the T-DNA or transposon ends (the “right” and “left” borders). All possible combinations of gene specific and T-DNA/transposon primers were used to detect by PCR an insertion event within or close to the target gene. The amplified DNA fragments were then sequenced which allows the precise determination of the T-DNA/transposon insertion point relative to the target gene. Insertion events within the coding or intervening sequence of the genes were deconvoluted from a pool comprising a plurality of insertion events to a single unique mutant plant for functional characterization. The method is described in more detail in Yu and Adam, U.S. application Ser. No. 09/177,733 filed Oct. 23, 1998.
Example VII
Identification of Modified Phenotypes in Overexpression or Gene Knockout Plants
Experiments were performed to identify those transformants or knockouts that exhibited modified biochemical characteristics. Among the biochemicals that were assayed were insoluble sugars, such as arabinose, fucose, galactose, mannose, rhamnose or xylose or the like; prenyl lipids, such as lutein, beta-carotene, xanthophyll-1, xanthophyll-2, chlorophylls A or B, or alpha-, delta- or gamma-tocopherol or the like; fatty acids, such as 16:0 (palmitic acid), 16:1 (palmitoleic acid), 18:0 (stearic acid), 18:1 (oleic acid), 18:2 (linoleic acid), 20:0, 18:3 (linolenic acid), 20:1 (eicosenoic acid), 20:2, 22:1 (erucic acid) or the like; waxes, such as by altering the levels of C29, C31, or C33 alkanes; sterols, such as brassicasterol, campesterol, stigmasterol, sitosterol or stigmastanol or the like, glucosinolates, protein or oil levels.
Fatty acids were measured using two methods depending on whether the tissue was from leaves or seeds. For leaves, lipids were extracted and esterified with hot methanolic H 2 SO 4 and partitioned into hexane from methanolic brine. For seed fatty acids, seeds were pulverized and extracted in methanol:heptane:toluene:2,2-dimethoxypropane:H 2 SO 4 (39:34:20:5:2) for 90 minutes at 80° C. After cooling to room temperature the upper phase, containing the seed fatty acid esters, was subjected to GC analysis. Fatty acid esters from both seed and leaf tissues were analyzed with a SUPELCO SP-2330 column (Supelco, Bellefonte, Pa.).
Glucosinolates were purified from seeds or leaves by first heating the tissue at 95° C. for 10 minutes. Preheated ethanol:water (50:50) is added and after heating at 95° C. for a further 10 minutes, the extraction solvent is applied to a DEAE SEPHADEX column (Pharmacia) which had been previously equilibrated with 0.5 M pyridine acetate. Desulfoglucosinolates were eluted with 300 ul water and analyzed by reverse phase HPLC monitoring at 226 nm.
For wax alkanes, samples were extracted using an identical method as fatty acids and extracts were analyzed on a HP 5890 GC coupled with a 5973 MSD. Samples were chromatographically isolated on a J&W DB35 mass spectrometer (J&W Scientific Agilent Technologies, Folsom, Calif.).
To measure prenyl lipid levels, seeds or leaves were pulverized with 1 to 2% pyrogallol as an antioxidant. For seeds, extracted samples were filtered and a portion removed for tocopherol and carotenoid/chlorophyll analysis by HPLC. The remaining material was saponified for sterol determination. For leaves, an aliquot was removed and diluted with methanol and chlorophyll A, chlorophyll B, and total carotenoids measured by spectrophotometry by determining optical absorbance at 665.2 nm, 652.5 nm, and 470 nm. An aliquot was removed for tocopherol and carotenoid/chlorophyll composition by HPLC using a Waters μBondapak C18 column (4.6 mm×150 mm). The remaining methanolic solution was saponified with 10% KOH at 80° C. for one hour. The samples were cooled and diluted with a mixture of methanol and water. A solution of 2% methylene chloride in hexane was mixed in and the samples were centrifuged. The aqueous methanol phase was again re-extracted 2% methylene chloride in hexane and, after centrifugation, the two upper phases were combined and evaporated. 2% methylene chloride in hexane was added to the tubes and the samples were then extracted with one ml of water. The upper phase was removed, dried, and resuspended in 400 ul of 2% methylene chloride in hexane and analyzed by gas chromatography using a 50 m DB-5 ms (0.25 mm ID, 0.25 um phase, J&W Scientific).
Insoluble sugar levels were measured by the method essentially described by Reiter et al. (1999), Plant J. 12: 335–345. This method analyzes the neutral sugar composition of cell wall polymers found in Arabidopsis leaves. Soluble sugars were separated from sugar polymers by extracting leaves with hot 70% ethanol. The remaining residue containing the insoluble polysaccharides was then acid hydrolyzed with allose added as an internal standard. Sugar monomers generated by the hydrolysis were then reduced to the corresponding alditols by treatment with NaBH4, then were acetylated to generate the volatile alditol acetates which were then analyzed by GC-FID. Identity of the peaks was determined by comparing the retention times of known sugars converted to the corresponding alditol acetates with the retention times of peaks from wild-type plant extracts. Alditol acetates were analyzed on a Supelco SP-2330 capillary column (30 m×250 μm×0.2 μm) using a temperature program beginning at 180° C. for 2 minutes followed by an increase to 220° C. in 4 minutes. After holding at 220° C. for 10 minutes, the oven temperature is increased to 240° C. in 2 minutes and held at this temperature for 10 minutes and brought back to room temperature.
To identify plants with alterations in total seed oil or protein content, 150 mg of seeds from T2 progeny plants were subjected to analysis by Near Infrared Reflectance Spectroscopy (NIRS) using a Foss NirSystems Model 6500 with a spinning cup transport system. NIRS is a non-destructive analytical method used to determine seed oil and protein composition. Infrared is the region of the electromagnetic spectrum located after the visible region in the direction of longer wavelengths. ‘Near infrared’ owns its name for being the infrared region near to the visible region of the electromagnetic spectrum. For practical purposes, near infrared comprises wavelengths between 800 and 2500 nm. NIRS is applied to organic compounds rich in O—H bonds (such as moisture, carbohydrates, and fats), C—H bonds (such as organic compounds and petroleum derivatives), and N—H bonds (such as proteins and amino acids). The NIRS analytical instruments operate by statistically correlating NIRS signals at several wavelengths with the characteristic or property intended to be measured. All biological substances contain thousands of C—H, O—H, and N—H bonds. Therefore, the exposure to near infrared radiation of a biological sample, such as a seed, results in a complex spectrum which contains qualitative and quantitative information about the physical and chemical composition of that sample.
The numerical value of a specific analyte in the sample, such as protein content or oil content, is mediated by a calibration approach known as chemometrics. Chemometrics applies statistical methods such as multiple linear regression (MLR), partial least squares (PLS), and principle component analysis (PCA) to the spectral data and correlates them with a physical property or other factor, that property or factor is directly determined rather than the analyte concentration itself. The method first provides “wet chemistry” data of the samples required to develop the calibration.
Calibration of NIRS response was performed using data obtained by wet chemical analysis of a population of Arabidopsis ecotypes that were expected to represent diversity of oil and protein levels.
The exact oil composition of each ecotype used in the calibration experiment was performed using gravimetric analysis of oils extracted from seed samples (0.5 g or 1.0 g) by the accelerated solvent extraction method (ASE; Dionex Corp, Sunnyvale, Calif.). The extraction method was validated against certified canola samples (Community Bureau of Reference, Belgium). Seed samples from each ecotype (0.5 g or 1 g) were subjected to accelerated solvent extraction and the resulting extracted oil weights compared to the weight of oil recovered from canola seed that has been certified for oil content (Community Bureau of Reference). The oil calibration equation was based on 57 samples with a range of oil contents from 27.0% to 50.8%. To check the validity of the calibration curve, an additional set of samples was extracted by ASE and predicted using the oil calibration equation. This validation set counted 46 samples, ranging from 27.9% to 47.5% oil, and had a predicted standard error of performance of 0.63%. The wet chemical method for protein was elemental analysis (% N×6.0) using the average of 3 representative samples of 5 mg each validated against certified ground corn (NIST). The instrumentation was an Elementar Vario-EL III elemental analyzer operated in CNS operating mode (Elementar Analysensysteme GmbH, Hanau, Germany).
The protein calibration equation was based on a library of 63 samples with a range of protein contents from 17.4% to 31.2%. An additional set of samples was analyzed for protein by elemental analysis (n=57) and scanned by NIRS in order to validate the protein prediction equation. The protein range of the validation set was from 16.8% to 31.2% and the standard error of prediction was 0.468%.
NIRS analysis of Arabidopsis seed was carried out on between 40–300 mg experimental sample. The oil and protein contents were predicted using the respective calibration equations.
Data obtained from NIRS analysis was analyzed statistically using a nearest-neighbor (N-N) analysis. The N-N analysis allows removal of within-block spatial variability in a fairly flexible fashion, which does not require prior knowledge of the pattern of variability in the chamber. Ideally, all hybrids are grown under identical experimental conditions within a block (rep). In reality, even in many block designs, significant within-block variability exists. Nearest-neighbor procedures are based on assumption that environmental effect of a plot is closely related to that of its neighbors. Nearest-neighbor methods use information from adjacent plots to adjust for within-block heterogeneity and so provide more precise estimates of treatment means and differences. If there is within-plot heterogeneity on a spatial scale that is larger than a single plot and smaller than the entire block, then yields from adjacent plots will be positively correlated. Information from neighboring plots can be used to reduce or remove the unwanted effect of the spatial heterogeneity, and hence improve the estimate of the treatment effect. Data from neighboring plots can also be used to reduce the influence of competition between adjacent plots. The Papadakis N-N analysis can be used with designs to remove within-block variability that would not be removed with the standard split plot analysis ((Papadakis (1973) Inst. d'Amelior. Plantes Thessaloniki (Greece) Bull. Scientif . No. 23; Papadakis (1984) Proc. Acad. Athens 59: 326–342).
Experiments were performed to identify those transformants or knockouts that exhibited modified sugar sensing. For such studies, seeds from transformants were germinated on media containing 5% glucose or 9.4% sucrose which normally partially restrict hypocotyl elongation. Plants with altered sugar sensing may have either longer or shorter hypocotyls than normal plants when grown on this media. Additionally, other plant traits may be varied such as root mass.
Experiments may be performed to identify those transformants or knockouts that exhibited an improved pathogen tolerance. For such studies, the transformants are exposed to biotropic fungal pathogens, such as Erysiphe orontii , and necrotropic fungal pathogens, such as Fusarium oxysporum. Fusarium oxysporum isolates cause vascular wilts and damping off of various annual vegetables, perennials and weeds (Mauch-Mani and Slusarenko (1994) Molec Plant - Microbe Interact. 7: 378–383). For Fusarium oxysporum experiments, plants are grown on Petri dishes and sprayed with a fresh spore suspension of F. oxysporum . The spore suspension is prepared as follows: A plug of fungal hyphae from a plate culture is placed on a fresh potato dextrose agar plate and allowed to spread for one week. Five ml sterile water is then added to the plate, swirled, and pipetted into 50 ml Armstrong Fusarium medium. Spores are grown overnight in Fusarium medium and then sprayed onto plants using a Preval paint sprayer. Plant tissue is harvested and frozen in liquid nitrogen 48 hours post-infection.
Erysiphe orontii is a causal agent of powdery mildew. For Erysiphe orontii experiments, plants are grown approximately 4 weeks in a greenhouse under 12 hour light (20° C., ˜30% relative humidity (rh)). Individual leaves are infected with E. orontii spores from infected plants using a camel's hair brush, and the plants are transferred to a Percival growth chamber (20° C., 80% rh.). Plant tissue is harvested and frozen in liquid nitrogen 7 days post-infection.
Botrytis cinerea is a necrotrophic pathogen. Botrytis cinerea is grown on potato dextrose agar under 12 hour light (20° C., ˜30% relative humidity (rh)). A spore culture is made by spreading 10 ml of sterile water on the fungus plate, swirling and transferring spores to 10 ml of sterile water. The spore inoculum (approx. 105 spores/ml) is then used to spray 10 day-old seedlings grown under sterile conditions on MS (minus sucrose) media. Symptoms are evaluated every day up to approximately 1 week.
Sclerotinia sclerotiorum hyphal cultures are grown in potato dextrose broth. One gram of hyphae is ground, filtered, spun down and resuspended in sterile water. A 1:10 dilution is used to spray 10 day-old seedlings grown aseptically under a 12 hour light/dark regime on MS (minus sucrose) media. Symptoms are evaluated every day up to approximately 1 week.
Pseudomonas syringae pv maculicola (Psm) strain 4326 and pv maculicola strain 4326 was inoculated by hand at two doses. Two inoculation doses allows the differentiation between plants with enhanced susceptibility and plants with enhanced resistance to the pathogen. Plants are grown for 3 weeks in the greenhouse, then transferred to the growth chamber for the remainder of their growth. Psm ES4326 may be hand inoculated with 1 ml syringe on 3 fully-expanded leaves per plant (4½ wk old), using at least 9 plants per overexpressing line at two inoculation doses, OD=0.005 and OD=0.0005. Disease scoring is performed at day 3 post-inoculation with pictures of the plants and leaves taken in parallel.
In some instances, expression patterns of the pathogen-induced genes (such as defense genes) may be monitored by microarray experiments. In these experiments, cDNAs are generated by PCR and resuspended at a final concentration of ˜100 ng/ul in 3×SSC or 150 mM Na-phosphate (Eisen and Brown (1999) Methods Enzymol. 303: 179–205). The cDNAs are spotted on microscope glass slides coated with polylysine. The prepared cDNAs are aliquoted into 384 well plates and spotted on the slides using, for example, an x-y-z gantry (OmniGrid) which may be purchased from GeneMachines (Menlo Park, Calif.) outfitted with quill type pins which may be purchased from Telechem International (Sunnyvale, Calif.). After spotting, the arrays are cured for a minimum of one week at room temperature, rehydrated and blocked following the protocol recommended by Eisen and Brown (1999; supra).
Sample total RNA (10 μg) samples are labeled using fluorescent Cy3 and Cy5 dyes. Labeled samples are resuspended in 4×SSC/0.03% SDS/4 μg salmon sperm DNA/2 μg tRNA/50 mM Na-pyrophosphate, heated for 95° C. for 2.5 minutes, spun down and placed on the array. The array is then covered with a glass coverslip and placed in a sealed chamber. The chamber is then kept in a water bath at 62° C. overnight. The arrays are washed as described in Eisen and Brown (1999, supra) and scanned on a General Scanning 3000 laser scanner. The resulting files are subsequently quantified using IMAGENE, software (BioDiscovery, Los Angeles Calif.).
RT-PCR experiments may be performed to identify those genes induced after exposure to biotropic fungal pathogens, such as Erysiphe orontii , necrotropic fungal pathogens, such as Fusarium oxysporum , bacteria, viruses and salicylic acid, the latter being involved in a nonspecific resistance response in Arabidopsis thaliana . Generally, the gene expression patterns from ground plant leaf tissue is examined.
Reverse transcriptase PCR was conducted using gene specific primers within the coding region for each sequence identified. The primers were designed near the 3′ region of each DNA binding sequence initially identified.
Total RNA from these ground leaf tissues was isolated using the CTAB extraction protocol. Once extracted total RNA was normalized in concentration across all the tissue types to ensure that the PCR reaction for each tissue received the same amount of cDNA template using the 28S band as reference. Poly(A+) RNA was purified using a modified protocol from the Qiagen OLIGOTEX purification kit batch protocol. cDNA was synthesized using standard protocols. After the first strand cDNA synthesis, primers for Actin 2 were used to normalize the concentration of cDNA across the tissue types. Actin 2 is found to be constitutively expressed in fairly equal levels across the tissue types we are investigating.
For RT PCR, cDNA template was mixed with corresponding primers and Taq DNA polymerase. Each reaction consisted of 0.2 μl cDNA template, 2 μl 10× Tricine buffer, 2 μl 10× Tricine buffer and 16.8 μl water, 0.05 μl Primer 1, 0.05 μl, Primer 2, 0.3 μl Taq DNA polymerase and 8.6 μl water.
The 96 well plate is covered with microfilm and set in the thermocycler to start the reaction cycle. By way of illustration, the reaction cycle may comprise the following steps:
Step 1: 93° C. for 3 min;
Step 2: 93° C. for 30 sec;
Step 3: 65° C. for 1 min;
Step 4: 72° C. for 2 min;
Steps 2, 3 and 4 are repeated for 28 cycles;
Step 5: 72° C. for 5 min; and
STEP 6 4° C.
To amplify more products, for example, to identify genes that have very low expression, additional steps may be performed: The following method illustrates a method that may be used in this regard. The PCR plate is placed back in the thermocycler for 8 more cycles of steps 2–4.
Step 2 93° C. for 30 sec;
Step 3 65° C. for 1 min;
Step 4 72° C. for 2 min, repeated for 8 cycles; and
Step 5 4° C.
Eight microliters of PCR product and 1.5 μl of loading dye are loaded on a 1.2% agarose gel for analysis after 28 cycles and 36 cycles. Expression levels of specific transcripts are considered low if they were only detectable after 36 cycles of PCR. Expression levels are considered medium or high depending on the levels of transcript compared with observed transcript levels for an internal control such as actin2. Transcript levels are determined in repeat experiments and compared to transcript levels in control (e.g., non-transformed) plants.
Experiments were performed to identify those transformants or knockouts that exhibited an improved environmental stress tolerance. For such studies, the transformants were exposed to a variety of environmental stresses. Plants were exposed to chilling stress (6 hour exposure to 4–8° C.), heat stress (6 hour exposure to 32–37° C.), high salt stress (6 hour exposure to 200 mM NaCl), drought stress (168 hours after removing water from trays), osmotic stress (6 hour exposure to 3 M mannitol), or nutrient limitation (nitrogen, phosphate, and potassium) (nitrogen: all components of MS medium remained constant except N was reduced to 20 mg/l of NH 4 NO 3 ; phosphate: all components of MS medium except KH2PO 4 , which was replaced by K 2 SO 4 ; potassium: all components of MS medium except removal of KNO 3 and KH 2 PO 4 , which were replaced by NaH 4 PO 4 ).
Experiments were performed to identify those transformants or knockouts that exhibited a modified structure and development characteristics. For such studies, the transformants were observed by eye to identify novel structural or developmental characteristics associated with the ectopic expression of the polynucleotides or polypeptides of the invention.
Flowering time was measured by the number of rosette leaves present when a visible inflorescence of approximately 3 cm is apparent. Rosette and total leaf number on the progeny stem are tightly correlated with the timing of flowering (Koornneef et al. (1991) Mol. Gen. Genet. 229: 57–66). The vernalization response was also measured. For vernalization treatments, seeds were sown to MS agar plates, sealed with micropore tape, and placed in a 4° C. cold room with low light levels for 6–8 weeks. The plates were then transferred to the growth rooms alongside plates containing freshly sown non-vernalized controls. Rosette leaves were counted when a visible inflorescence of approximately 3 cm was apparent.
Modified phenotypes observed for particular overexpressor or knockout plants are provided in Table 4. For a particular overexpressor that shows a less beneficial characteristic, it may be more useful to select a plant with a decreased expression of the particular transcription factor. For a particular knockout that shows a less beneficial characteristic, it may be more useful to select a plant with an increased expression of the particular transcription factor.
The sequences of the Sequence Listing or those in Tables 4–9, or those disclosed here, can be used to prepare transgenic plants and plants with altered traits. The specific transgenic plants listed below are produced from the sequences of the Sequence Listing, as noted. Tables 4 and 6 provide exemplary polynucleotide and polypeptide sequences of the invention.
Example VIII
Examples of Genes that Confer Significant Improvements to Plants
Examples of genes and homologs that confer significant improvements to knockout or overexpressing plants are noted below. Experimental observations made by us with regard to specific genes whose expression has been modified in overexpressing or knock-out plants, and potential applications based on these observations, are also presented.
This example provides experimental evidence for increased biomass and abiotic stress tolerance controlled by the transcription factor polypeptides and polypeptides of the invention.
Salt stress assays are intended to find genes that confer better germination, seedling vigor or growth in high salt. Evaporation from the soil surface causes upward water movement and salt accumulation in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt concentration in the whole soil profile. Plants differ in their tolerance to NaCl depending on their stage of development, therefore seed germination, seedling vigor, and plant growth responses are evaluated.
Osmotic stress assays (including NaCl and mannitol assays) are intended to determine if an osmotic stress phenotype is NaCl-specific or if it is a general osmotic stress related phenotype. Plants tolerant to osmotic stress could also have more tolerance to drought and/or freezing.
Drought assays are intended to find genes that mediate better plant survival after short-term, severe water deprivation. Ion leakage will be measured if needed. Osmotic stress tolerance would also support a drought tolerant phenotype.
Temperature stress assays are intended to find genes that confer better germination, seedling vigor or plant growth under temperature stress (cold, freezing and heat).
Sugar sensing assays are intended to find genes involved in sugar sensing by germinating seeds on high concentrations of sucrose and glucose and looking for degrees of hypocotyl elongation. The germination assay on mannitol controls for responses related to osmotic stress. Sugars are key regulatory molecules that affect diverse processes in higher plants including germination, growth, flowering, senescence, sugar metabolism and photosynthesis. Sucrose is the major transport form of photosynthate and its flux through cells has been shown to affect gene expression and alter storage compound accumulation in seeds (source-sink relationships). Glucose-specific hexose-sensing has also been described in plants and is implicated in cell division and repression of “famine” genes (photosynthetic or glyoxylate cycles).
Germination assays followed modifications of the same basic protocol. Sterile seeds were sown on the conditional media listed below. Plates were incubated at 22° C. under 24-hour light (120–130 μEin/m 2 /s) in a growth chamber. Evaluation of germination and seedling vigor was conducted 3 to 15 days after planting. The basal media was 80% Murashige-Skoog medium (MS)+vitamins.
For salt and osmotic stress germination experiments, the medium was supplemented with 150 mM NaCl or 300 mM mannitol. Growth regulator sensitivity assays were performed in MS media, vitamins, and either 0.3 μM ABA, 9.4% sucrose, or 5% glucose.
Temperature stress cold germination experiments were carried out at 8° C. Heat stress germination experiments were conducted at 32° C. to 37° C. for 6 hours of exposure.
For stress experiments conducted with more mature plants, seeds were germinated and grown for seven days on MS+vitamins+1% sucrose at 22° C. and then transferred to chilling and heat stress conditions. The plants were either exposed to chilling stress (6 hour exposure to 4–8° C.), or heat stress (32° C. was applied for five days, after which the plants were transferred back 22° C. for recovery and evaluated after 5 days relative to controls not exposed to the depressed or elevated temperature).
Results:
G12 (SEQ ID NO: 3)
Published Information
G12 (At4g36900) is on chromosome 4, contig fragment No. 86, GenBank accession number AL161590 (nid=7270623). The gene has been described as RAP2.10 by Okamuro et al., (1997) Proc Natl Acad Sci 94, 7076–7081.
Experimental Observations
G12 was determined to be ubiquitously expressed in plants. The function of G12 was studied using a line homozygous for a T-DNA insertion in the gene. G12 knockout mutant seedlings germinated in the dark on ACC-containing media (an ethylene insensitivity assay) were more severely stunted than the wild-type controls. These results indicate that G12 is involved in the ethylene signal transduction or response pathway, a process in which other proteins of the AP2/EREBP family are implicated. G12 KO mutant plants were wild-type in morphology and development, and in all other physiological and biochemical analyses that were performed.
In addition to the knockout mutant, the function of the gene was analyzed using transgenic plants in which the cDNA clone for G12 was expressed under the control of the 35S promoter. Overexpression of G12 caused seedlings to develop black necrotic tissue patches on cotyledons and seedlings died before the formation of true leaves. Some 35S::G12 overexpressing seedlings exhibited a weaker phenotype characterized by smaller necrotic patches on leaf margins. However, those plants arrested growth and died before flowering. No seed was obtained from any of the G12 overexpressing lines.
Utilities
The overexpression and knockout phenotypes indicate that G112 can have a role in regulating programmed cell death. Such a function could have various applications. The gene, its equivalogs, or its targets could be used to induce cell death in a controlled manner in specific tissues or in response to pathogen attack. For example, if the gene was specifically active in gametes or reproductive organs, it might be used to achieve male or female sterility. Alternatively, in the latter scenario, it might restrict the spread of a pathogen infection through a plant.
G30 (SEQ ID NO: 7)
Published Information
G30 (At1g04370) is part of the BAC clone F19P19, GenBank accession number AC000104 (nid=2341023).
Experimental Observations
Initial experiments were performed with G30 knockout mutant plants. However, these experiments did not uncover the functions of the gene.
In order to characterize the gene further, 35S::G30 overexpressing lines were generated. Morphological analysis of the transgenic plants indicated that G30 could be involved in light regulation: the seedlings had long hypocotyls and elongated cotyledon petioles. In addition, some of the seedlings also had longer roots compared to control plants. At later stages, the plants became darker green, and had glossy leaves, perhaps indicating elevated levels of epidermal wax. The phenotype for G30 overexpression resembled those produced by related AP2 genes.
Utilities
Based on the appearance of 35S::G30 leaves, the gene could be used to engineer changes in the composition and amount of leaf surface components (most likely wax). The ability to manipulate wax composition, amount, or distribution could modify plant tolerance to drought and low humidity, or resistance to insects or pathogens. Additionally, in some species, wax is a valuable commodity and altering its accumulation and/or composition could enhance yield.
The phenotypes of 35S::G30 seedlings indicate that the gene may also be used to manipulate light-regulated developmental processes like shade avoidance. Eliminating shading responses might allow increased planting densities with subsequent yield enhancement.
Additionally, if the dark coloration of 35S::G30 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G46 (SEQ ID NO: 9)
Published Information
G46 was first identified in the sequence of P1 clone MBK20 (GenBank accession number AB010070, gene MBK20.1). No information is available about the function(s) of G46.
Experimental Observations
RT-PCR experiments revealed that G46 was ubiquitously expressed, but was potentially induced by stress conditions such as auxin, heat, salt and Erysiphe.
The function of G46 was studied using a line homozygous for a T-DNA insertion in the gene. G46 knockout mutant plants were indistinguishable from wild-type in all assays performed.
The function of G46 was also analyzed using transgenic plants in which a cDNA clone of the gene was expressed under the control of the 35S promoter. A number of lines were larger than wild-type plants, developed more rapidly, and yielded an increased quantity of seed compared to wild-type controls.
In the physiological analysis, all three 35S::G46 lines (number 32, 35, and 36) tested showed more resistance to severe water deprivation stress. Seedlings were generally larger and greener than the control plants exposed to the same conditions.
35S::G46 plants were also significantly larger and greener in a soil-based drought assay than wild-type control plants.
Utilities
The reduced sensitivity of 35S::G46 lines in the dehydration stress assay indicated that the gene or its equivalogs might be used to engineer crops with increased tolerance to drought, salt, freezing and/or chilling stress, or increased water use efficiency.
Additionally, the increased size and growth rate seen in some of the lines indicated that the gene or its equivalogs can be used to increase crop productivity.
G47 (SEQ ID NO: 11)
Published Information
G47 corresponds to gene T22J18.2 (AAC25505). No information is available about the function(s) of G47.
Experimental Observations
The function of G47 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G47 resulted in a variety of morphological and physiological phenotypic alterations.
35S::G47 plants showed enhanced tolerance to osmotic stress. In a root growth assay on PEG containing media, G47 overexpressing transgenic seedlings were larger and had more root growth compared to the wild-type controls ( FIG. 3A ). Interestingly, G47 expression levels might be altered by environmental conditions, in particular reduced by salt and osmotic stresses. In addition to the phenotype observed in the osmotic stress assay, germination efficiency for the seeds from G47 overexpressors was low.
35S::G47 plants were also significantly larger and greener in a soil-based drought assay than wild-type controls plants.
Overexpression of G47 also produced a substantial delay in flowering time and caused a marked change in shoot architecture. 35S::G47 transformants were small at early stages and switched to flowering more than a week later than wild-type controls (continuous light conditions). Interestingly, the inflorescences from these plants appeared thick and fleshy, had reduced apical dominance, and exhibited reduced internode elongation leading to a short compact stature ( FIG. 3B ). The branching pattern of the stems also appeared abnormal, with the primary shoot becoming ‘kinked’ at each coflorescence node. Additionally, the plants showed slightly reduced fertility and formed rather small siliques that were borne on short pedicels and held vertically, close against the stem.
Additional alterations were detected in the inflorescence stems of 35S::G47 plants. Stem sections from T2-21 and T2-24 plants were of wider diameter, and had large irregular vascular bundles containing a much greater number of xylem vessels than wild type. Furthermore some of the xylem vessels within the bundles appeared narrow and were possibly more lignified than were those of controls.
G47 was expressed at higher levels in rosette leaves, and transcripts can be detected in other tissues (flower, embryo, silique, and germinating seedling), but apparently not in roots.
Utilities
G47 or its equivalogs could potentially be used to manipulate flowering time, to modify plant architecture and stem structure, including development of vascular tissues and lignin content, and to improve plant performance under drought and osmotic stress conditions.
The use of G47 or its equivalogs from tree species could offer the potential for modulating lignin content. This might allow the quality of wood used for furniture or construction to be improved.
G148 (SEQ ID NO: 39)
Published Information
G148 corresponds to AGAMOUS-LIKE 13 (AGL13), and was originally identified based on its conserved MADS domain (Purugganan et al. (1995) Genetics 140: 345–356; Rounsley et al. (1995). Plant Cell 7: 1259–1269). No functional information about G148 is available in the public domain. However, its expression pattern indicated that the gene has a role in ovule development; AGL13 transcript was present in ovules at the time of integument development, but fell following fertilization. Additionally, lower levels of expression were found in anther filaments and style tissue (Rounsley et al. (1995) supra).
Experimental Observations
Homozygotes were analyzed for a transposon insertion (SLAT collection) within G148; these plants showed no obvious macroscopic changes in morphology and exhibited a similar response to wild type in all of the physiological assays performed.
The effects of G148 overexpression were studied by generating transgenic lines in which a G148 genomic clone was expressed from the 35S CaMV promoter. 35S::G148 transformants displayed a range of morphological changes including a severe reduction in overall plant size, leaf curling, accelerated flowering, and terminal flower formation. Such changes indicate that G148 influences the genetic networks controlling various aspects of development including flowering time and meristem determinacy.
Utilities
The morphological changes seen in the overexpression lines demonstrate that G148 could be used to manipulate various aspects of plant development.
The appearance of terminal flowers in 35S::G148 transformants indicated that the gene or its orthologs can modify inflorescence architecture and confer a determinate habit in species where the shoots otherwise show an indeterminate growth pattern. Such changes completely alter the overall plant form, and may, for example, facilitate mechanical harvesting (as already exemplified by the SELF-PRUNING gene, which controls shoot determinacy in tomato, Pnueli L et al. (1998). Development 125: 1979–1989).
Additionally, the accelerated switch to reproductive growth seen in 35S::G148 plants, indicated that the gene can be used to manipulate flowering time in commercial species. Specifically, the gene can accelerate flowering or eliminate any requirement for vernalization. In some instances, a faster cycling time might allow additional harvests of a crop to be made within a given growing season. Shortening generation times can also help speed-up breeding programs, particularly in species such as trees, which grow for many years before flowering.
G151 (SEQ ID NO: 41)
Published Information
G151 corresponds to AGL15, a gene isolated by virtue of its conserved MADS box sequence (Rounsley et al. (1995). Plant Cell 7: 1259–1269) and by its preferential expression in young Brassica napus embryos (Heck et al. (1995) Plant Cell 7:1271–1282). On the basis of AGL15 expression patterns, it has been suggested that this gene might be involved in embryogenesis (Heck et al., 1995; supra, Perry et al. (1996) Plant Cell 8:1977–1989; Perry et al. (1999) Plant Physiol. 120:121–130). In addition, overexpression of AGL15 has been shown to inhibit perianth organ senescence and abscission (Fernandez et al. (2000) Plant Cell 12:183–198). However, G151/AGL15 still remains poorly characterized, and the gene likely has multiple roles.
G151 is expressed preferentially during embryogenesis and accumulates during early seed development (Perry et al., 1996, supra). AGL15-specific antibodies were used to demonstrate that AGL15 accumulates before fertilization in the cytoplasm of cells of the egg apparatus and moves into the nucleus during early stages of development in the suspensor, embryo, and endosperm (Perry et al., 1996; 1999, supra). Relatively high levels of AGL15 are present in the nuclei during embryo morphogenesis and until the seeds start to dry in Brassica , maize, and Arabidopsis . It has also been shown that AGL15 is associated with the chromosomes during mitosis, and gel mobility shift assays were used to demonstrate that the protein binds DNA in a sequence-specific manner (Perry et al., 1996, supra).
AGL15 expression, however, is not restricted to embryonic tissues. It has been found that the AGL15 protein accumulates transiently in the shoot apices of young Arabidopsis and Brassica seedlings, and that promoter activity is associated with the shoot apex and the base of leaf petioles throughout the vegetative phase (Fernandez et al. (2000) supra). In addition, during the reproductive phase, AGL15 accumulates transiently in floral buds (Fernandez et al. (2000) supra). When AGL15 was expressed in Arabidopsis under the control of a strong constitutive promoter, delayed abscission of perianth organs in the flowers was noted, changes in leaf shape occurred, and some age-dependent developmental processes (including the transition to flowering and fruit maturation) were delayed (Fernandez et al. (2000) supra).
Experimental Observations
The function of G151 was analyzed using a line that was homozygous for a T-DNA insertion within the gene. However, these plants displayed no consistent differences to wild type in any of the assays performed. We surmised that this could be due to potential redundancy between G151 and a highly related gene, G858 (AGL18). (G858 and G151 from a monophyletic clade within the Arabidopsis MADS box gene family (Alvarez-Buylla et al. (2000) Plant J. 24:457–466).
RT-PCR experiments also indicated that G151 is expressed ubiquitously, with the highest levels occurring in embryo and silique tissues. These results confirmed and expanded previously published observations describing that AGL15/G151 is preferentially expressed in the developing embryo, and also in germinating seedlings and leaf tissue (Rounsley et al. (1995) supra; Heck et al., (1995) supra; Perry et al., (1996, 1999) supra). In addition, G151 expression appeared to be induced by auxin.
35S::G151 overexpressing lines displayed a wild-type response in all of the physiological assays. Furthermore, although our lines expressed the transgene (determined by RT-PCR), for unknown reasons, we failed to recapitulate the effects on petal and sepal abscission, leaf shape, and flowering time that had been obtained by Fernandez et al. (2000) supra. It is noteworthy that Fernandez et al. attained much more pronounced results, and detected much higher levels of AGL15 protein, in 35S::AGL15 lines that harbored a genomic clone of the gene, rather than a cDNA clone. All of the 35S::G151 lines we created contained a cDNA clone; it is possible that the transcript from this transgene was less stable those in the ‘genomic lines’ of Fernandez et al. (2000). The discrepancy in results might also derive from differences in the lengths of 5′ UTR included in the overexpression construct, or differences in the strengths of different versions of the 35S promoters that were used.
Interestingly, however, in a small number of lines we noted an effect, which was not explicitly mentioned by Fernandez et al. (2000); the seeds were larger than were those of wild type. It is not clear, though, whether this could have been related to delayed seed ripening, which Fernandez et al. documented, or whether it was due to some other aspect of the G151 role in seed or embryo development.
Utilities
Based on the publicly available data, G151 or its equivalogs could likely be used to manipulate age related developmental processes such as flowering time, seed maturation and floral organ retention. The latter trait might be of particular interest to the ornamental plant industry and might allow the ‘campaign life’ of flowers to be extended.
This gene or its equivalogs may also be used to increase seed size and yield. The promoter of G151 might be useful for engineering auxin-inducible expression.
G153 (SEQ ID NO: 43)
Published Information
G153 corresponds to the Arabidopsis ANR1 gene. This locus was identified by Zhang and Forde (1998) as a MADS box gene that is rapidly induced in the roots of nitrogen starved seedlings, following exposure to a nitrate source. Additionally, it was shown that transgenic lines in which an antisense clone of ANR1 is overexpressed show altered sensitivity to nitrate and, unlike wild-type plants, do not exhibit lateral root proliferation in response to nitrate treatments. From these data, it was concluded that ANR1 is a key regulator of nutrient-induced changes in root architecture (Zhang and Forde (1998) Science 279: 407–409).
However, Wang et al. ((2000) Plant Cell 12, 1491–1509) have data that contradicts the results of Zhang and Forde (1998). These authors found that ANR1 is actually repressed, rather than induced, following treatment of nitrogen starved seedlings (grown on 10 mM ammonium succinate as the sole nitrogen source) with 5 mM nitrate.
A phylogenetic analysis of the Arabidopsis MADS box gene family situated ANR1 in same clade as three other MADS box genes: AGL16 (G860), AGL17 (G152) and AGL21 (G1760) (Alvarez-Buylla et al. (2000) Proc Natl Acad Sci U.S.A. 97: 5328–5333). Two of the genes, AGL17 and AGL21 were recently shown to be expressed in specific zones of the root, indicating that different members of the ANR1 clade may play distinct regulatory roles during root development (Burgeff et al. (2002 Planta 214: 365–372).
The ANR1 sequence (GenBank accession AX507709) has also been included in a patent publication (WO0216655A) by Harper et al. (2002).
Experimental Observations
RT-PCR experiments revealed that G153 is up-regulated in leaves in response to heat and Fusarium treatments. Lower levels of induction were also observed following auxin, ABA, and cold treatments, indicating that G153 might have a role in a variety of stress responses.
To further assess the function of the gene, 35S::G153 overexpressing lines were generated and subjected to a suite of assays. Around a third of the lines showed a marked acceleration in the onset of flowering, indicating that the gene might impinge on genetic pathways that regulate flowering time.
In addition to the effects on flowering, 35S::G153 lines displayed an enhanced performance in an assay intended to reveal alterations in C/N sensing. 35S::G153 seedlings contained less anthocyanin and in a number of cases were larger than wild-type controls grown on high sucrose/N-plates. Seedlings were also larger and greener on high sucrose/N-plates that had been supplemented with glutamine. Together, these data indicated that overexpression of G153 may alter the ability to modulate carbon and/or nitrogen uptake and utilization.
It should be noted that a closely related gene, G1760, prior to the C/N sensing assay being implemented. Like 35S::G153 transformants, 35S::G1760 lines also exhibited early flowering, and RT-PCR studies showed G1760 to be predominantly expressed in roots and to be stress responsive. Thus, G1760 and G153 could have similar and/or overlapping functions.
Utilities
The response of G153 expression to different physiological treatments indicates that the gene or its equivalogs could be used to improve resistance to a variety of different stresses. In particular, the enhanced performance of 35S::G153 lines under low nitrogen conditions indicated that G153 might be used to engineer crops that could thrive in environments with reduced nitrogen availability.
The finding that 35S::G153 lines make less anthocyanin on high sucrose media containing glutamine indicated that G153 or its equivalogs might be used to modify carbon and nitrogen status, and hence alter assimilate partitioning.
Given the early flowering seen amongst the 35S::G153 transformants, the gene or its equivalogs might also be applied to manipulate the flowering time of commercial species. In particular, G153 could be used to accelerate flowering, or eliminate any requirement for vernalization.
G155 (SEQ ID NO: 45)
Published Information
G155 corresponds to AGAMOUS-LIKE 8 (AGL8), and was originally identified based on its conserved MADS domain (Mandel et al. (1995) Plant Cell 7: 1763–1771). The gene behaves as an early marker of the switch to reproductive growth; AGL8 RNA is not present during vegetative growth, but accumulates to high levels in the inflorescence apical meristem, as well as in the inflorescence stem and cauline leaves (Mandel et al. supra; Hempel et al. (1997) Development 124: 3845–3853). Additionally, AGL8 RNA is excluded from the young flower primordia that arise on the flanks of the inflorescence meristem (Mandel et al. supra). Such expression patterns indicate that AGL8 could have a role in maintaining the separation between inflorescence and floral meristem identity. Later, AGL8 RNA also accumulates in the walls of the developing carpels (Gu et al. (1998) Development 125: 1509–1517), indicating that it functions in fruit development. This has been confirmed through the study of mutants; the gene was found to have a major role in fruit valve differentiation. Loss of function mutants form siliques that lack coordinated growth, often fail to dehiscence, show premature rupture of the carpel valves, and become overcrowded with seeds (Gu et al. (1998) supra). Based on this phenotype, the gene was renamed FRUITFUL (FUL). Overexpression lines for FUL also show abnormalities in silique shattering and it has been shown that the gene acts as an inhibitor of the SHATTERPROOF (SHP1, SHP2; GIDs G136, G140) genes (Ferrandiz et al. (2000) Science 289: 436–438).
Experimental Observations
To determine whether G155 has functions additional to its known roles in fruit development, overexpression lines were generated and subjected to a battery of assays. 35S::G155 transformants exhibited early flowering and a number of lines also developed terminal flowers, giving a phenotype very similar to that exhibited by the terminal flower 1 mutant (Shannon et al. (1991) Plant Cell 3: 877–892; Bradley et al. (1997) Science 275: 80–83. Alterations in silique development in the transformed lines were not detected. However, given that such effects are thought to occur through inhibition of the SHP genes, it is possible that the G155 expression levels in the 35S::G155 lines were not sufficiently high enough to elicit such effects, compared to the 35S::FUL lines reported by Ferrandiz et al. (2000), supra.
In addition to the changes in morphology, when subjected to physiological assays, 35S::G155 transformants showed increased sensitivity to osmotic stress in germination assays on either glucose or mannitol plates.
Utilities
Based on its published function, G155 can manipulate flower and fruit traits in commercial species. The overexpression data o demonstrated that G155 or its orthologs could be used to manipulate various other aspects of plant development.
The appearance of terminal flowers in 35S::G155 transformants indicated that the gene can modify inflorescence architecture and confer a determinate habit in species where the shoots show an indeterminate growth pattern. Such changes can completely alter the overall plant form, and can facilitate mechanical harvesting (as exemplified by the SELF-PRUNING gene, which controls shoot determinacy in tomato, Pnueli et al. (1998), supra).
Additionally, the rapid switch to reproductive growth seen in 35S::G155 plants, indicated that the gene can manipulate flowering time in commercial species. The gene could be used to accelerate flowering or eliminate any requirement for vernalization. In some instances, a faster cycling time might allow additional harvests of a crop to be made within a given growing season. Shortening generation times could also help speed-up breeding programs, particularly in species such as trees, which grow for many years before flowering.
The results of physiological assays on 35S::G155 plants indicate that the transcription factor can be used to manipulate abiotic stress responses or to modify source/sink relationships or other sugar regulated processes.
G200 (SEQ ID NO: 53)
Published Information
G200 corresponds to gene AT1g08810, and has been described as MYB60 (Kranz et al. (1998) Plant J. 16: 263–276). Expression analysis by reverse Northern blot indicates that G200 is slightly induced only in silique tissue and by IAA, cold and UV treatments (Kranz et al., 1998). No information is available about the function(s) of G200.
Experimental Observations
Analysis of a G200 knockout demonstrated that homozygous G200 knockout plants were phenotypically wild-type. G200 was determined to be ubiquitously expressed in Arabidopsis , contradicting the report by Kranz et al., supra.
Overexpression of G200 produced pleiotropic effects on plant development, causing alterations in overall plant size, coloration, flowering time, leaf shape, flower structure and fertility.
Relatively few 35S::G200 overexpressing lines were obtained; only 10 T1 plants were recovered from 4 separate 300 mg aliquots of T0 seed (a 300 mg aliquot typically yields 15–120 T1 plants), indicating that G200 can be lethal in plants that highly overexpress the gene.
The 35S::G200 lines that were isolated were generally very small, slightly pale in coloration, had rather pointed and/or contorted leaves, and abnormal phyllotaxy. A number of the lines also produced flower buds slightly earlier than wild type. Additionally, the flowers of overexpressing G200 plants were typically smaller and gaped open more widely than those of controls. Interestingly, in an assay intended to determine whether the transgene expression could alter C:N sensing, 35S::G200 seedlings contained less anthocyanins, and in some cases were greener, than wild-type controls grown on high sucrose/N deficient plates. This indicates that some of the growth defects observed in these lines were related to the carbon or nutrient availability in different growth substrates. Seedlings were also greener on high sucrose/nitrogen deficient/glutamine-supplemented plates. These data together indicate that overexpression of G200 may alter a plant's ability to modulate carbon and/or nitrogen uptake and utilization.
Utilities
The enhanced performance of G200 overexpression lines under low nitrogen conditions indicate that the gene could be used to engineer crops that could thrive under conditions of reduced nitrogen availability. Such a trait would afford the following benefits: (1) cost savings to the farmer by reducing the amount of fertilizer needed (2) environmental benefits of reduced fertilizer run-off into watersheds (3) improved yield and stress tolerance.
That 35S::G200 lines make less anthocyanin on high sucrose plus glutamine, indicates G200 might be used to modify carbon and nitrogen status, and hence assimilate partitioning.
G319 (SEQ ID NO: 71)
Published Information
G319 (At1g05290) was identified in the sequence of YAC yUP8H12, GenBank accession number AC000098, released by the Arabidopsis Genome Initiative based on its sequence similarity within the conserved domain to other CONSTANS-like related proteins in Arabidopsis . There is no published or public information about G319.
Experimental Observations
Low levels of G319 expression were detected only in embryo and siliques. No expression of G319 was detected by RT-PCR in any other tissues or conditions tested. The function of G319 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G319 produced plants with short broad leaves and delayed flowering. 35S::G319 plants were wild-type in physiological analyses that were performed.
Utilities
G319 could be used to alter flowering time or produce plants with altered leaf morphology.
The delayed flowering displayed by 35S::G319 transformants indicates that the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases. In some species (for example sugar beet), where the vegetative parts of the plant constitute the crop, it would be advantageous to delay or suppress flowering in order to prevent resources being diverted into reproductive development. Additionally, delaying flowering beyond the normal time of harvest could alleviate the risk of transgenic pollen escape from such crops.
Given the effects of G319 overexpression, it is likely that the activity of the gene (or its orthologs) could be modified to accelerate flowering, or eliminate any requirement for vernalization.
G354 (SEQ ID NO: 1381)
Published Information
G354 was identified in the sequence of BAC clone F12M12, GenBank accession number AL355775, released by the Arabidopsis Genome Initiative. G354 corresponds to ZAT7 (Meissner and Michael (1997) Plant Mol. Biol. 33: 615–624).
Experimental Observations
The highest level of expression of G354 was observed in rosette leaves, embryos, and siliques. Some expression of G354 was also observed in flowers.
The function of this gene was analyzed using transgenic plants in which G353 was expressed under the control of the 35S promoter. 35S::G354 plants had a reduction in flower pedicel length, and downward pointing siliques. This phenotype was very similar to that described for the brevipedicellus (bp) mutant (Koornneef et al. (1983) J. Hered. 74: 265–272) and in overexpression of a related gene G353. Other morphological changes in shoots were also observed in 35S::G354 plants. Many 35S::G354 seedlings had abnormal cotyledons, elongated, thickened hypocotyls, and short roots. The majority of T1 plants had a very extreme phenotype, were tiny, and arrested development without forming inflorescences. T1 plants showing more moderate effects had poor seed yield.
Overexpression of G354 in Arabidopsis resulted in seedlings with an altered response to light. In a germination assay conducted in darkness, G354 seedlings failed to show an etiolation response, as can been seen in FIG. 4 which shows G354 overexpressing and wild-type seedlings germinated on MS plates in the dark. In some cases the phenotype was severe; overexpression of the transgene resulted in reduced open and greenish cotyledons.
G354 overexpressors were also shown to be tolerant to water deprivation in a soil-based drought assay. Closely related paralogs of this gene, G353 and G2839, also showed an osmotic stress tolerance phenotype in a germination assay on media containing high sucrose; one line of 35S::G353 seedlings and several lines of 35S::G2839 were greener and had higher germination rates than controls. Thus, G354 and its paralogs G353 and G2839 appear to influence osmotic stress responses.
Utilities
G354 could be used to alter inflorescence structure, which may have value in production of novel ornamental plants.
G355 (SEQ ID NO: 79)
Published Information
G355 was identified in the sequence of BAC F3G5 (GenBank accession number AC005896; gene At2g37430), released by the Arabidopsis Genome Initiative. G355 is also known as ZAT11 (Meissner and Michael (1997) Plant Mol. Biol. 33:615–624). No information has been published about the function of this gene.
Experimental Observations.
G355 expression was found to be weakly induced in rosette leaves by ABA treatment, drought stress, osmotic stress and infection by Erysiphe . An attempt to determine the function of G355 was analyzed using transgenic plants in which the expression of this gene was knocked out using a T-DNA insertion. However, these plants appeared wild-type in all morphological, physiological and biochemical assays performed.
The function of G355 was then studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. G355 overexpressing lines show more tolerance to salt stress and enhanced growth under limiting phosphate in root growth assays. Seedlings in both assays were larger, greener and had more root growth. 35S::G355 plants were wild-type in morphological analyses that were performed.
Utilities
Based on the increased salt tolerance exhibited by the 35S::G355 lines in physiology assays, this gene might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions. The trait is of particular importance early in the life cycle, since evaporation from the soil surface causes upward water movement, and salt accumulates in the upper soil layer where the seeds are placed. Thus, germination normally takes place at a salt concentration much higher than the mean salt level in the whole soil profile. Increased salt tolerance during the germination stage of a crop plant would therefore enhance survivability and yield.
The response of 35S::G355 seedlings to low phosphate conditions indicates that the gene could be used to manipulate nutrient uptake, or the ability to grow in poor nutrient soils. Phosphorus is a limiting nutrient in plant growth and is often added to soil as fertilizer. Young plants have a rapid intake of phosphate and sufficient phosphate is important for yield of root crops such as carrot, potato and parsnip. Phosphate costs represent a relatively small but significant portion of farmers' operating costs (3–4% of total costs to a corn farmer in the US, higher to a vegetable grower). Plants that are tolerant to phosphate deficiency could represent a cost saving for farmers, especially in areas where soils are very poor. They could also provide environmental benefits by reducing pollution from field runoff.
G370 (SEQ ID NO: 83)
Published Information
G370 was initially described as ZFP8, one of a set of C2H2 zinc finger proteins (Tague and Goodman (1995) Plant Mol. Biol. 28: 267–279). No functional information is available about ZFP8.
Experimental Observations
G370 was shown to be expressed throughout the aerial portions of the plant, but showed no detectable expression in roots. It was not induced by any condition tested. A knockout line homozygous for a T-DNA insertion in G370 was initially used to determine the function of this gene and showed more sensitivity to osmotic stress in a germination assay. Thus, when selectively regulated, the gene could have utility in creating plants with enhanced tolerance to dehydration stresses.
The function of G370 was also studied using overexpressing transgenic plants in which the gene was expressed under the control of the 35S promoter. All 35S::G370 primary transformants were small. Flowers showed a striking increase in trichome density on sepals, and carried ectopic trichomes on petals, anthers, and carpels. The changes in morphology produced by overexpression of G370 are indicative of heterochronic shifts (i.e. cells in various lineages and tissues adopt fates that are normally associated with cells from other developmental stages). For example, trichomes are normally associated with vegetative rather than reproductive organs. Additionally, aerial rosettes occur when a secondary inflorescence meristem develops in a manner comparable to a primary shoot meristem during the vegetative phase of growth.
Utilities
G370 is expressed throughout the aerial portions of the plant, but shows no detectable expression in roots. It was not induced by any condition tested. A line homozygous for a T-DNA insertion in G370 was initially used to determine the function of this gene and showed more sensitivity to osmotic stress in a germination assay and displayed defects in leaf and inflorescence development.
The function of G370 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. All 35S::G370 primary transformants were small and stunted. Flowers showed a striking increase in trichome density on sepals, and carried ectopic trichomes on petals, anthers, and carpels. Because insufficient seed was produced by 35S::G370 plants, no physiological analyses were performed.
G370 is closely related to five other Z-C2H2 genes: G2826, G1995, G361, G362, and G2838, which produced broadly similar phenotypes when overexpressed, such as ectopic trichomes on flowers, aerial rosettes, and various other morphological defects. The changes in morphology produced by overexpression of genes in this clade are suggestive of heterochronic shifts (i.e. cells in various lineages and tissues adopt fates that are normally associated with cells from other developmental stages). For example, trichomes are normally associated with vegetative rather than reproductive organs. Additionally, aerial rosettes occur when a secondary inflorescence meristem develops in a manner comparable to a primary shoot meristem during the vegetative phase of growth.
G372 (SEQ ID NO: 85)
Published Information
G372 was identified in the sequence of BAC clone T6D20, GenBank accession number U90439, released by the Arabidopsis Genome Initiative. There is no other published or public information about the function of G372.
Experimental Observations
As determined by RT-PCR, G372 is highly expressed under all environmental conditions tested. The function of G372 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G372 in Arabidopsis delayed the onset of flowering resulted in plants with increased in leaf size and plant biomass compared to control plants. Leaf size was twice that of the controls in many cases
Utilities
Given the effects of G372 overexpression, the gene or its orthologs could be used to modify leaf size and flowering time. Increasing leaf size in crop plants through the activity of G372 could result in the direct increase in yield in situations where the vegetative tissues are the harvested products. In addition, the increases in leaf surface area attributed to G372 activity could also result in yield increases in fruit bearing or seed bearing crops due to the increase in the photosynthetic capacity of larger leaves. In species such as sugarbeet where the vegetative parts of the plants constitute the crop and the reproductive tissues are discarded, it would be advantageous to delay or prevent flowering. Extending vegetative development can bring about large increases in yields. Additionally, a major concern is the escape of transgenic pollen from GMOs to wild species or so-called organic crops. Systems that prevent or delay vegetative transgenic crops from flowering could be used to mitigate this concern.
G438 (SEQ ID NO: 97)
Published Information
G438 was identified as a homeobox gene (MUP 24.4) within P1 clone MUP 24 (GenBank accession number AB005246). We have also identified G438 as the Arabidopsis REVOLUTA (REV) gene (Ratcliffe et al. (2000) Plant Cell 12: 315–317). Based on its mutant phenotype, REV had previously been identified as having a key role in regulating the relative growth of apical versus non-apical (cambial) meristems (Alvarez, J. (1994). The SPITZEN gene. In Arabidopsis: An atlas of morphology and development , ed., J. Bowman). pp. 188–189. New York: Springer-Verlag); Talbert et al. (1995) Development 121: 2723–2735). The revoluta phenotype is highly pleiotropic but is characterized by a failure in development of all types of apical meristem: lateral shoot meristems in the axils of cauline and rosette leaves are often completely absent, or replaced by a solitary leaf. These effects are most evident in higher order shoots, but in some cases, the primary shoot meristem also fails and terminates growth in a cluster of filamentous structures. Rev floral meristems often fail to complete normal development and form incomplete or abortive filamentous structures. In contrast to apical meristems, structures formed by non-apical meristems, such as leaves, stems and floral organs often become abnormally large and contorted in the rev mutant.
The features of rev mutants are similar to those of the interfascicular fiberless1 (ifl1) mutant. Ifl1 was isolated during screens for mutants lacking normal stem fiber differentiation (Zhong et al. (1997) Plant Cell 9, 2159–2170). Wild-type Arabidopsis plants form interfascicular fibers which become lignified and add support to the inflorescence stem (Aloni et al. (1987) Annu. Rev. Plant Physiol. 38: 179–204; Zhong et al. (1997) supra; Zhong et al. (1999) Plant Cell 11: 2139–2152. In the ifl1 mutant, normal interfascicular fibers are absent and the differentiation of both xylary fibers and vessel elements is disrupted. In additions to these internal features, ifl1 mutants have secondary morphological features very similar to those of rev. Recently the IFL1 gene was cloned by Zhong et al. (1999) supra. We have found that the IFL1 sequence and map position are identical to those of the REV gene cloned by us, demonstrating that REV and IFL1 are in fact, the same gene (Ratcliffe et al. (2000) supra).
It has been suggested that REV promotes the growth of apical meristems (including floral meristems) at the expense of non-apical meristems (Talbert et al. (1995) supra). It is not yet clear, however, whether expression data support such a role: strong expression of REV has been detected in interfascicular regions and developing vascular tissue, but in-situ expression analysis of apical meristems has not yet been reported (Zhong et al. (1999) supra). REV is a group III HD-ZIP protein and shares high sequence similarity (and organization) with the proteins encoded by three other Arabidopsis genes: Athb8, Athb9, and Athb14 (Sessa et al. (1998) Plant Mol. Biol. 38: 609–622). It is possible, therefore, that these genes act together in the same developmental process.
Closely Related Genes from Other Species
Blast searches reveal that the Physcomitrella patens homeobox protein PpHB10 has a relatively high degree of sequence identity to REV. The function of PpHB10 has not been published but it contains 465 conserved amino acid identities to REV across its 880 amino acid sequence. The existence of this similar protein in such a distantly related species suggests that potential orthologs from dicots or monocots would be expected to have a much greater degree of identity to REV over their sequences.
Experimental Observations
G438 was initially identified as MUP24.4, a novel putative homeobox gene within P1 clone MUP24 (GenBank Accession AB005246). Annotation was confirmed by isolation of the G438 cDNA: the cDNA had an in-frame stop codon immediately 5′ to the predicted start codon and comprised 18 exons that had been predicted within the genomic sequence.
Plants homozygous for a T-DNA insertion in the G438 sequence were obtained by PCR based screening of DNA pools from the Jack Collection of insertional mutants (Campisi et al. (1999) Plant Journal 17, 699–707). The T-DNA insertion was located 466 bp downstream of the putative start codon, and was predicted to create a null mutation. The mutation was recessive and produced a revoluta phenotype. The most prominent characteristic was a failure in the development of all types of apical meristem: lateral shoot meristems in the axils of cauline and rosette leaves were often completely absent, or replaced by a solitary leaf. These effects were most evident in higher order shoots, but in some cases, the primary shoot meristem also failed and terminated growth in a cluster of filamentous structures. Overall, the mutant had a dramatic reduction in branching at maturity compared to wild-type plants. The T-DNA insertion mutant also showed delayed senescence, had enlarged revolute leaves, long pendent stems, and exhibited floral meristem defects whereby flowers had enlarged organs, altered organ numbers, or sporadically failed to develop and were replaced by filamentous structures.
The similarity between the phenotype of KOG438 and that described for revoluta raised the possibility that the two genes were allelic. This possibility was strengthened by the fact that the rev mutation mapped to a region of chromosome 5 close to MUP 24 (Talbert et al. (1995) supra. To examine this, we obtained mutants homozygous for the rev-1 and rev-1011 alleles (kindly supplied by E. Meyerowitz) and compared their phenotype to the KOG438 mutant. The features of these mutants were very similar to those of KOG438. The most prominent characteristic of these mutants was a reduction in branching in the inflorescence. Populations of rev-1, KO438, rev-1011, and wild type (Col) plants were grown under continuous light conditions and the inflorescences examined at approximately 5 weeks after sowing. The structures present in axils of the cauline leaves on the primary shoot and the cauline leaves on secondary shoots (i.e. paraclades borne by the primary shoot) were noted (Table 10). Cauline leaf axils either contained a leaf, a shoot or were empty. The total number of visible shoots on the entire plant was also recorded.





TABLE 10



Structures present in axils of the cauline leaves on the primary

shoot and the cauline leaves on secondary shoot of G438

overexpressors



Wild type Columbia (12 plants scored at approx.

5 weeks from sowing)




primary cauline (26 on 12 plants)
secondary cauline (60 on 12 plants):



100% contained shoots
95% contained shoots

0% contained leaves
0% contained leaves

0% empty
5% empty



Mean total number of visible shoots = 45 +/− 8

rev-1 (14 plants scored at approx. 5 weeks from sowing)

primary cauline (32 on 14 plants)
secondary cauline (24 on 14 plants):



25% contained shoots
0% contained shoots

3% contained leaves
0% contained leaves

72% empty
100% empty



Mean total number of visible shoots = 3.5 +/− 0.8

rev-1011 (6 plants scored at approx. 5 weeks from sowing)

primary cauline (10 on 6 plants)
secondary cauline (2 on 6 plants):



10% contained shoots
0% contained shoots

20% contained leaves
0% contained leaves

70% empty
100% empty



Mean total number of visible shoots = 2.2 +/− 1.2

KOG438 (27 plants scored at approx. 5 weeks from sowing)

primary cauline (38 on 27 plants)
secondary cauline (55 on 27 plants):



71% contained shoots
4% contained shoots

3% contained leaves
4% contained leaves

26% empty
92% empty



Mean total number of visible shoots = 3.4 +/− 0.6



















































We concluded that rev-1, rev-1011, and KO438 plants all exhibited a severe reduction in the development of secondary and higher order shoots compared to wild type at this flowering stage. Rev-1 and rev-1011 had a slightly stronger phenotype than KO438 based on the number of cauline leaves bearing shoots. The rev-1 and rev-1011 alleles had been isolated in a Nossen background. A batch of rev-1 were therefore grown alongside wild-type Nossen in continuous light conditions. The wild-type Nossen plants were noted to develop a similar architecture to the wild type Columbia plants in the previous experiment. The plants were examined at approximately 10 weeks after sowing in this second experiment. At this time rev-1 plants had 9.7+/−1.6 and Nossen wild type had 55+/−5 visible shoots. The additional shoots on the rev-1 plants at 10 weeks compared to 5 weeks were mainly axillary shoots that had grown out from the basal rosette. Only 2/61 of these rosette inflorescences had any side shoots.
To check whether G438 was the REVgene we isolated the G438 sequence from rev-1, rev-1011 wild type Columbia and wild type Nossen. The G438 sequence from rev-1 and rev-1011 were found to be identical, indicating that both were the same allele! These sequences exhibited eight single-base changes compared to that from wild type Nossen (and 9 differences compared to wild type Columbia, due to a single base polymorphism between Nossen and Columbia in the 5th intron). Of these eight changes, one was upstream of the putative start codon, four were present in putative introns, and two were present in the 3′ UTR. The final change was a G to A substitution predicted to disrupt the splice site at the junction between the eleventh intron and the twelfth exon. To confirm the intron-exon boundaries, the G438 cDNA sequence was isolated by PCR from cDNA derived from a mixture of tissues. The gene consisted of 18 exons, which were predicted to encode an 842 amino acid homeodomain leucine-zipper protein. The splice-site mutation in G438 from rev-1 was expected to prevent removal of the eleventh intron, resulting in an aberrant transcript.
The above result strongly suggested that G438 was REV gene. We therefore performed a genetic complementation test and crossed homozygotes for the KOG438 with rev-1 and rev-1011 homozygotes, and wild type plants. All 20 F1 plants from the cross to wild type had a wild-type phenotype. Twenty F1 plants from the KOG438 x rev-1 population and 20 F1 plants from the KOG438 x rev-1011 population all exhibited a revoluta phenotype. These data confirmed that G438 was the REV gene.
The T-DNA collection from which KOG438 was derived contained a GUS reporter gene construct. We stained heterozygous KOG438 plants with GUS to see whether a tissue specific expression pattern for G438 would be revealed. GUS staining was not noted in wild type controls at any stage. In seedlings containing KO438, no staining was seen before 3 days after sowing. From 5–8 days after sowing, strong staining was visible in the axils of rosette leaves in positions where secondary shoots were developing. Strong expression was not noted in the primary apex. These expression patterns correlate well with the enhanced deficiencies in axillary shoot development (compared to the primary shoot) in the rev mutant. It is possible that there is an increased requirement for REV in axillary shoots (compared to the primary) to ensure their proper initiation and outgrowth. GUS staining was also noted in the vascular tissue, roots (but not root tips), and in the stigmae and pedicels of flowers. (To verify that GUS staining was due to the T-DNA inserted in G438 and not some other background T-DNA, selfed seed was collected from F1 plants in the KO438 x wt population. In the F2 population, 48 plants were resistant and 17 were sensitive to kanamycin. This 3:1 segregation suggested that the T-DNA was inserted at a single locus, i.e. within G438).
Shortly after we isolated the REV gene, the cloning of INTERFASCICULAR FIBERLESS1 (IFL1) was reported (Zhong et al. (1999) supra). IFL1 was found to be the same gene as REV, but had been studied independently and under a different name (Ratcliffe et al. (2000) supra). The salient feature of the ifl1 mutant was an absence of lignified interfascicular fiber cells in the stem (although it was noted to have features such as enlarged curled leaves). In wild type, these cells can be visualized by phloroglucinol staining, but are absent from the mutant (Zhong et al. (1997) supra). To examine whether these cells were absent, stem sections were cut from revoluta plants (F1 from the KOG438 x rev-1 and KOG438 x rev-1011 crosses) and wild type plants and stained with phloroglucinol. Lignified interfascicular fiber cells could be seen stained purple in the wild type but were absent from the revoluta mutants, confirming that rev has an ifl1 phenotype.
The finding that IFL1 is REVOLUTA might help explain the deficiencies in fiber differentiation in the mutant. Lignified fiber cells are essential in providing support for the plant stem, and are thought to develop in response to the polar auxin flow which originates at the shoot tips (Aloni (1987) supra; Zhong (1999) supra). IFL1 was proposed to act either by regulating polar auxin flow or by regulating the genes involved in the transduction of hormonal signals that trigger fiber differentiation. REVOLUTA is considered to be essential for apical meristem development. Since the auxin stream that induces fiber differentiation derives from shoots, it seems reasonable to suggest that defects in shoot meristem development would alter the polar auxin flow, and as a consequence, influence fiber differentiation. Thus, the interfascicular fiber-less phenotype of the rev mutant may be an indirect effect of the apical meristem deficiencies.
The precise role of REV still remains elusive. It has been suggested that REV promotes the growth of apical meristems (including floral meristems) at the expense of non-apical (cambial) meristems (Talbert et al. (1995) supra). It is not yet clear, however, whether expression data supports such a role: strong expression of REV has been detected in interfascicular regions and developing vascular tissue, but detailed in-situ expression analysis of apical meristems has not yet been reported (Zhong et al. (1999) supra). REV is a group III HD-ZIP protein and shares high sequence similarity (and organization) with the proteins encoded by three other Arabidopsis genes being studied: G392 (Athb8), G390 (Athb9), and G391 (Athb14) (Sessa et al. (1998) supra). It is possible, therefore, that these genes act together in the same developmental process. Supporting this suggestion, Athb8 has a similar expression pattern to REV and is transcribed in the procambial regions of vascular bundles (Baima et al. (1995) supra). Thus, to gain a full understanding of REV function and its contribution to plant architecture, it will be necessary to study the gene in conjunction with the other homologs. To further this aim we are now studying G438 alongside G392. A homozygous population of KOG392 plants has recently been obtained. The KOG392 plants display a wild-type morphology and exhibit a wild-type staining pattern with phloroglucinol. Crosses are now being made to obtain the KOG438; KOG392 double mutant. We are also in the process of producing overexpressors for G438 and G392. It is hoped that these studies will provide a greater understanding of the function of G438 and thereby allow us to engineer plants with a modified stem lignin content or altered patterns of branching.
RT-PCR analyses detected G438 expression at medium to high levels in all tissues and conditions tested. Further expression analysis was possible, however, since the T-DNA insertion contained an enhancer trap construct (Campisi et al. (1999) supra). GUS staining could therefore be used to reveal the expression pattern of genes within which insertions occurred. GUS staining of seedlings homozygous and heterozygous for the G438 T-DNA insertion revealed very strong expression within axillary shoots. This expression data has not yet been confirmed by other methods, but correlates with the marked effects of the rev mutation on outgrowth of higher order shoots.
Utilities
The mutant phenotypes indicate that REV/IFL1 has an important role in determining overall plant architecture and the distribution of lignified fiber cells within the stem. A number of utilities can be envisaged based upon these functions.
(1) Modification of Lignin Composition
Modifying the activity of REVOLUTA orthologs from tree species could offer the potential for modulating lignin content. This might allow the quality of wood used for furniture or construction to be improved.
(2) Modification of Plant Architecture
In Arabidopsis , reduced REV activity results in a reduction of higher-order shoot development. Reducing activity of REV orthologs might generate trees that lack side branches, and have fewer knots in the wood.
G485 (SEQ ID NO: 105)
Published Information
G485 is a member of the Hap3-like subfamily of CCAAT-box binding transcription factors. G485 corresponds to gene At4g14540, annotated by the Arabidopsis Genome Initiative. The gene corresponds to sequence 1042 from Patent Application WO0216655 on stress-regulated genes, transgenic plants and methods of use, in which G485 was reported to be cold responsive in a microarray analysis (Harper et al. (2002) Patent Application WO0216655). No information is available about the function(s) of G485.
Experimental Observations
RT-PCR analyses of the endogenous levels of G485 indicated that this gene is expressed in all tissues and under all conditions tested. Homozygotes for a T-DNA insertion allele of G485 flowered several days later than control plants. G485 was then overexpressed, and gain of function and loss of function studies on G485 revealed opposite effects on flowering time. Under conditions of continuous light, approximately half of the 35S::G485 primary transformants flowered distinctly up to a week earlier than wild-type controls. These effects were observed in each of two independent T1 plantings derived from separate transformation dates. These studies indicate that G485 acts as a floral activator and is also necessary in that role within the plant.
Utilities
Based on the loss of function and gain of function phenotypes, G485 or its orthologs could be used to modify flowering time.
The delayed flowering displayed by G485 knockouts indicated that the gene or its orthologs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
The early flowering effects seen in the G485 overexpressors could be applied to accelerate flowering, or eliminate any requirement for vernalization.
G581 (SEQ ID NO: 115)
Published Information
G581 was first identified as Atmyc1 by Urao et al. (1996) Plant Mol. Biol. 32: 571–576. It has been shown that its transcripts were more abundant in developing seeds than in stems and leaves. G581 contains a Sph box (CATGCATG) in its promoter region that is known as a cis-regulatory element conferring seed-specific expression. No other information regarding G581 function is available in the literature.
Experimental Observations
Using an RT-PCR-based approach, it was determined that G581 was uniformly expressed in all tissues tested, and the expression level was unchanged by all of the environmental conditions or pathogens infections tested.
The function of G581 was first studied by knockout analysis. Homozygous plants containing a T-DNA insertion within the first half of the G581 coding region displayed wild-type morphology at all developmental stages. Furthermore, G581 knockout mutant plants behaved similarly to wild type in all physiological and biochemical assays performed.
The function of G581 was also assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from the 35S CaMV promoter. Overexpression of G581 resulted in plants with alterations in seed coloration, and a mild delay in the onset of flowering. Seeds from 35S::G581 transgenic lines were pale and larger compared to wild-type controls. In addition, G581 overexpressing lines germinated better on plates containing low nitrogen or plates with low nitrogen supplemented with glutamine. Under such conditions, seedlings also had less measurable anthocyanin accumulation when compared to wild-type controls.
Utilities
The enhanced growth of G581 overexpression lines under low nitrogen conditions indicate that the gene could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
G581 could be used to alter anthocyanin production or accumulation. This could enhance the health benefits of foodstuffs, could be used to alter pigment production for horticultural purposes, or possibly increase resistance to a variety of stresses.
Additionally, the delayed flowering displayed by 35S::G581 transformants indicates that the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Given the effects of G581 overexpression, it is likely that the activity of the gene (or its orthologs) could also be modified to accelerate flowering, or eliminate any requirement for vernalization.
Finally, the changes in size and coloration shown by 35S::G581 seeds indicate that the gene might be used to enhance seed traits or yield.
G624 (SEQ ID NO: 119 and SEQ ID NO: 2105)
Published Information
G624 was identified in the sequence of BAC F18E5, GenBank accession number AL022603, released by the Arabidopsis Genome Initiative.
Experimental Observations
Overexpression of G624 produced a moderate delay in the onset of flowering (approximately one week under continuous light conditions). A number of the late flowering 35S::G624 transformants also displayed a marked increase in vegetative biomass compared to controls. No altered phenotypes were detected in any of the physiological assays.
Intriguingly, overexpression lines containing a truncated form of the cDNA (SEQ ID NO: 2105) exhibited wild-type morphology but displayed enhanced tolerance to both high sodium chloride and low phosphate growth conditions. It is possible that this effect represents a dominant negative phenotype.
Utilities
The delayed flowering displayed by 35S::G624 transformants indicated that the gene or its equivalogs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth or an increase in leaf size can significantly increase biomass and result in substantial yield increases.
Based on the increased salt tolerance exhibited by the 35S::G624 lines in physiology assays, this gene or its equivalogs might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
The response of 35S::G624 seedlings to low phosphate conditions indicated that the gene or its equivalogs could be used to manipulate nutrient uptake or the ability to grow in poor nutrient soils.
G627 (SEQ ID NO: 121)
Published Information
G627 corresponds to AGAMOUS-LIKE 19 (AGL19) which was isolated by Alvarez-Buylla et al. (2000) Plant J. 24: 457–466. No genetic characterization of AGL19 has been reported, but it was found to be specifically expressed in the outer layers of the root meristem (lateral root cap and epidermis) and in the central cylinder cells of mature roots (Alvarez-Buylla et al. (2000), supra).
Experimental Observations
RT-PCR expression studies failed to detect G627 in any of the tissue types analyzed. This result partially agrees with the data of Alvarez-Buylla et al. (2000), supra, who found that the gene is expressed only in specific regions of the root. It is possible that such regions were not sufficiently represented, for G627 transcript to be detected in the whole root samples analyzed in expression studies. In later experiments, however, a G627 clone was isolated by high cycle PCR from a cDNA sample derived from mixed tissues, and transgenic lines were generated in which this clone was expressed from a 35S promoter.
A substantial proportion of the 35S::G627 lines flowered markedly earlier than control plants. Such effects were observed in both the T1 and T2 generations and indicate that the gene plays a role in the regulation of flowering time.
Utilities
Given the early flowering seen amongst the 35S::G627 transformants, the gene or its orthologs may be used to manipulate the flowering time of commercial species. In particular, G627 could be used to accelerate flowering, or eliminate any requirement for vernalization.
G651 (SEQ ID NO: 125 and SEQ ID NO: 2106)
Published Information
G651 was identified in the sequence of BAC T7123, GenBank accession number U89959, released by the Arabidopsis Genome Initiative. There is no other published or public information about G651.
Experimental Observations
The function of G651 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Two clones were used to determine the function of G651. One clone, P15159, contained no errors compared with the publicly annotated sequence. Another clone, P2812, lacked a glutamic acid residue at position 203 and was 3′ truncated, lacking the final 8 amino acids (SEQ ID NO: 2106). The conserved domains were present in both clones.
Equivalent morphological effects were observed in 35S::G651 lines containing either of the different overexpression constructs. Overexpression of G651 produced a number of alterations in Arabidopsis growth and development, including changes in leaf morphology, overall size, growth rate, and fertility. Leaves of 35S::G651 plants had a dark grayish appearance, were often contorted and had an abnormal undulated surface texture. It is possible that such features could reflect changes in leaf cuticle composition/deposition or alterations in the histology of the epidermis. 35S::G651 lines were generally small, slow developing, displayed retarded inflorescence outgrowth, and often had poorly developed flowers with multiple non-specific abnormalities.
G651 (P15159) overexpressing lines behave similarly to the wild-type controls in all physiological assays performed. However, in general, overexpression of G651 caused deleterious effects on plant growth. G651 seedlings were small and vitrified. One line also accumulated anthocyanins. G651 (P2812, comprising SEQ ID NO: 2106) overexpressing lines showed an additional effect and exhibited increased sensitivity to cold stress in a germination assay. Furthermore, 35S::G651 lines harboring P2812 displayed little or no secondary root growth.
Utilities
Depending on the basis of the color change seen in 35S::G651 lines, a number of applications could be envisaged. If the phenotype is due to loosening of epidermal cell layers, the gene or its equivalogs might be used to produce fruits, vegetables, and other plant products that can be more easily peeled. If the effects are due to changes in wax composition/accumulation, G651 or its equivalogs might be used to afford protection against pests or abiotic stresses such as drought. If, however, the phenotype is due to changes in pigment levels within the leaf, the gene or its equivalogs might be applied to alter photosynthetic capacity and yield.
The changes in root development seen in 35S::G651 lines indicated that the gene or its equivalogs could be used to manipulate root growth and thereby influence the uptake of water and nutrients.
The altered response to cold germination assays indicated that the gene or its equivalogs might be applied to modify abiotic stress responses.
G652 (SEQ ID NO: 127)
Published Information
G652 (At1g14580) was identified in the sequence of BAC T5E21, GenBank accession number AC010657, released by the Arabidopsis Genome Initiative based on its sequence similarity within the conserved domain to other Zinc CLDSH related proteins in Arabidopsis.
G652 was described in the literature as atGRP2 (de Oliveira et al. (1990) Plant Cell. 2: 427–436). The authors describe atGRP2 as being rich in glycine and not induced by ethylene, abscisic acid, salicylic acid, water stress or drought. Kingsley and Palis (1994) Plant Cell 6: 1522–1523) noted that atGRP2 contains a cold shock domain and two zinc fingers.
Closely Related Genes from Other Species
G652 is glycine rich and shares homology with other GRP proteins found in plants in addition to the cold shock domain and zinc finger domain.
Experimental Observations
G652 appears to be constitutively expressed at medium levels in all tissues and environmental conditions tested as determined by RT-PCR analysis. Expression of G652 was not detected in other tissues. A line homozygous for a T-DNA insertion in G652 was used to determine the function of this gene. The T-DNA insertion of G652 is approximately 75% into the coding sequence of the gene and therefore is likely to result in a null mutation. Plants homozygous for a T-DNA insertions within G652 displayed a spectrum of developmental abnormalities, particularly at the early seedling stage. These phenotypes were variable within the population suggesting that other factors might be influencing the penetrance of the phenotype. For example, seedlings were small and filled with anthocyanins. Almost all the seedlings had defects in cotyledons ranging from unusual shape to fusions. Many seedlings did not survive. Those that did grew slowly. Fertility was reduced compared to controls, senescence delayed, and siliques were often rather short. The reason for this poor fertility was unclear. Many flowers had a reduced number of stamens (4–5 of these organs rather than 6). Interestingly, the absent stamen(s) were usually one or both of the shorter pair. Seeds produced by knockouts of G652 plants were somewhat wrinkled and misshapen.
The G652 knockout line had a reproducible increase in the leaf glucosinolate M39480. It also showed a reproducible increase in seed alpha-tocopherol. A decrease in seed oil as measured by NIR was also observed, but the values were slightly above the cutoff value for statistical significance.
The function of G652 was also studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G652 resulted in plants that were small and slow developing. Many plants died at an early stage of growth. The two lines that were morphologically examined in the T2 generation were small and showed premature senescence of rosette leaves.
Utilities
G652 could be used to manipulate seed tocopherol composition and seed structure and to alter glucosinolate composition in leaves. Tocopherols have anti-oxidant and vitamin E activity. Increases or decreases in specific glucosinolates or total glucosinolate content might be desirable depending upon the particular application. For example: (1) Glucosinolates are undesirable components of the oilseeds used in animal feed, since they produce toxic effects. Low-glucosinolate varieties of canola have been developed to combat this problem. (2) Some glucosinolates have anti-cancer activity; thus, increasing the levels or composition of these compounds might be of interest from a nutraceutical standpoint. (3) Glucosinolates form part of a plants natural defense against insects. Modification of glucosinolate composition or quantity could therefore afford increased protection from predators. Furthermore, in edible crops, tissue specific promoters might be used to ensure that these compounds accumulate specifically in tissues, such as the epidermis, which are not taken for consumption.
Based on the overexpression data, G652 could be used to manipulate plant growth and development. In particular, the accelerated senescence of the 35S::G652 lines indicates that the gene could be used to modify disease responses, or alter the rate of senescence in crops.
G807 (SEQ ID NO: 141)
Published Information
The heat shock transcription factor G807 is referred to in the public literature as the Arabidopsis HSF3, a class-A HSF characterized by an extended HR-A/B oligomerization domain (Nover et al. (1996) Cell Stress 1:215–223). G807 is found in the sequence of the chromosome 5 BAC clone F5E19 (GenBank accession number AL391147.1; nid=9755718), released by the Arabidopsis Genome Initiative. The translation start and stop codons were incorrectly predicted in the BAC annotation. Overexpression of the Arabidopsis HSF3 or HSF3-GUS fusion protein results in the constitutive expression of the heat shock proteins and in an increase in the basal thermotolerance in transgenic plants (Prandl et al. (1998) Mol. Gen. Genet. 258: 269–278).
Experimental Observations
RT-PCR analysis of the endogenous level of G807 transcripts revealed a moderate but constitutive level in all tissue examined. G807 transcript level increased moderately upon heat shock and auxin treatment, but decreased below detectable level following salt treatment. Analysis of a G807 null mutant reveals no apparent morphological, physiological or biochemical changes when compared to control plants.
The function of G807 was analyzed through its ectopic overexpression in Arabidopsis . A number of beneficial phenotypes were observed in the transgenic 35S::G807 overexpressor lines that have not been previously reported in the scientific literature. The seedling vigor was generally improved in primary T1 transformants and in the T2 progenies. Seedlings germinated on agar-MS plate under 12 hr light were reproducibly larger and showed longer hypocotyl than control plants. This phenotype was highly penetrant. The long petiole effect was observed in the primary transformants but was not apparent in any of the T2 progenies characterized.
Physiological analysis of 35S::G807 overexpressor lines revealed increased seedling vigor in a cold germination assay (MS-agar, 8° C., 3–15 days). Germinated seedlings were generally larger and accumulated less anthocyanin than control plants treated under the same conditions. This phenotype was observed in a primary screen using a mixed line population, as well as in repeated treatment with individual lines.
Utilities
Based on published data, G807 might be used to improve heat tolerance.
From the experimental studies performed by us, a number of other potential applications are apparent:
(1) G807 could be used to confer chilling tolerance
The growth of many crops is very sensitive to cool temperatures. A gene that enhances growth under chilling conditions could result in enhanced yields. For example, chilling may lead to yield losses and lower product quality through the delayed ripening of maize. Photoinhibition of photosynthesis (disruption of photosynthesis due to high light intensities) often occurs under clear atmospheric conditions subsequent to cold late summer/autumn nights. Another consequence of poor growth is the rather poor ground cover of maize fields in spring, often resulting in soil erosion, increased occurrence of weeds, and reduced uptake of nutrients. A retarded uptake of mineral nitrogen could lead to increased losses of nitrate into the ground water. Enhanced chilling tolerance could also extend the effective growth range of chilling sensitive crop species by allowing earlier planting or later harvest.
Chilling tolerance could also serve as a model for understanding how plants adapt to water deficit. Both chilling and water stress share similar signal transduction pathways and tolerance/adaptation mechanisms. For example, acclimation to chilling temperatures can be induced by water stress or treatment with abscisic acid. Genes induced by low temperature include dehydrins (or LEA proteins). Dehydrins are also induced by salinity, abscisic acid, water stress and during the late stages of embryogenesis.
Another large impact of chilling occurs during post-harvest storage. For example, some fruits and vegetables do not store well at low temperatures (for example, bananas, avocados, melons, and tomatoes). The normal ripening process of the tomato is impaired if it is exposed to cool temperatures. Genes conferring resistance to chilling temperatures may enhance tolerance during post-harvest storage.
(2) G807 could be used to accelerate seedling growth, and thereby allow a crop to become established faster. This would minimize exposure to stress conditions at early stages of growth, when the plants are most sensitive. Additionally, it might allow a crop to become grow faster than competing weed species.
(3) G807 might be used to manipulate light responses such as shade avoidance.
G839 (SEQ ID NO: 145)
Published Information
G839 was identified by amino acid sequence similarity to plant and mammalian ankyrin-repeat proteins. G839 is found in the sequence of the chromosome 5, TAC clone: K17O22 (GenBank accession number AB019224.1, nid=3869063), released by the Arabidopsis Genome Initiative. G839 has no other distinctive feature besides the presence of a 33-AA repeated ankyrin element known for protein—protein interaction, in the C-terminus of the predicted protein.
The G839 product is closely related to NPR1, a gene that controls the onset of systemic acquired resistance in plant (Cao et al. (1997) Cell 88:57–63; Cao et al. (1998) Proc. Natl. Acad. Sci. 95: 6531–6536). However, no information related to the functional characterization of G839 is currently available from the public literature.
Experimental Observations
RT-PCR studies revealed that G839 is expressed throughout the plant, with the lowest levels in germinating seedlings.
The function of G839 was analyzed through its ectopic overexpression in Arabidopsis; 35S::G839 lines displayed a delay in the onset of flowering (1–7 days), but were otherwise morphologically similar to wild-type control plants. In addition, 35S::G839 lines showed increased vigor and had more secondary root growth than controls when grown on plates containing low nitrogen.
Utilities
Nitrogen is the major nutrient affecting plant growth and development that ultimately impacts yield and stress tolerance. Plants of the G839 overexpressing lines grown under low nitrogen conditions were larger, showed enhanced primary and secondary root growth, and less chlorosis compared to the control plants. In some cases, twice as much root and shoot biomass was observed in the G839 transgenics plants when compared to a comparable wild-type plant, indicating that the gene or its orthologs could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
The delayed flowering in 35S::G839 lines indicated that the gene or its orthologs can manipulate flowering time. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
G916 (SEQ ID NO: 153)
Published Information
G916 corresponds to gene At4g04450, and it has also been described as WRKY42. No information is available about the function(s) of G916.
Experimental Observations
The complete cDNA sequence of G916 was experimentally determined. G916 appears to be expressed at low levels in a range of tissues, and was not significantly induced by any of the conditions tested.
A T-DNA insertion mutant for G916, displayed wild-type morphology. Overexpression of G916 produced a wide spectrum of developmental abnormalities in Arabidopsis . Many of the 35S::G916 seedlings were extremely tiny and showed an apparent lack of shoot organization. Such plants arrested growth and died at very early stages. Other individuals were small and displayed disproportionately long hypocotyls and narrow cotyledons. At later stages, the majority of surviving lines were markedly smaller than wild type, and formed rather weedy inflorescence stems that yielded very few flowers. Additionally, flowers often had poorly developed organs.
In addition, G916 overexpressing lines were larger than control wild-type seedlings in several germination assays. Larger seedlings were observed under conditions of high sucrose. In addition, 35S::G916 seedlings were larger and appeared to have less anthocyanin on high sucrose plates that were nitrogen deficient, with or without glutamine supplementation. The assays monitor the effect of C on N signaling through anthocyanin production. That 35S::G916 seedlings perform better under conditions of high sucrose alone makes it more difficult to interpret the better seedling performance under conditions of low nitrogen. Tissue specific or inducible expression of this gene could aid in sorting out the complex phenotypes caused by the constitutive overexpression of this gene.
G916 is related to two other WRKY genes, G184 and G186. Members of this clade could have redundant function in Arabidopsis . Overexpression of G184 caused a variety of morphological alterations, similar to those of the 35S::G916 seedlings. Similar to the G916 KO mutant, G186 single knockout mutant plants did not show phenotypic alterations in the analyses preformed.
Utilities
The enhanced performance of G916 overexpression lines under low nitrogen conditions indicate that the gene could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
That 35S::G916 lines make less anthocyanin on high sucrose plus glutamine, indicates G916 might be used to modify carbon and nitrogen status, and hence assimilate partitioning.
The results of physiological assays indicate that G916 could be used to alter the sugar signaling in plants. In addition to their important role as an energy source and structural component of the plant cell, sugars are central regulatory molecules that control several aspects of plant physiology, metabolism and development. It is thought that this control is achieved by regulating gene expression and, in higher plants, sugars have been shown to repress or activate plant genes involved in many essential processes such as photosynthesis, glyoxylate metabolism, respiration, starch and sucrose synthesis and degradation, pathogen response, wounding response, cell cycle regulation, pigmentation, flowering and senescence. The mechanisms by which sugars control gene expression are not understood.
Because sugars are important signaling molecules, the ability to control either the concentration of a signaling sugar or how the plant perceives or responds to a signaling sugar could be used to control plant development, physiology or metabolism. For example, the flux of sucrose (a sugar used for systemically transporting carbon and energy in most plants) has been shown to affect gene expression and alter storage compound accumulation in seeds. Manipulation of the sucrose signaling pathway in seeds may therefore cause seeds to have more protein, oil or carbohydrate, depending on the type of manipulation. Similarly, in tubers, sucrose is converted to starch that is used as an energy store. It is thought that sugar-signaling pathways may partially determine the levels of starch synthesized in the tubers. The manipulation of sugar signaling in tubers could lead to tubers with higher starch content.
Thus, manipulating the sugar signal transduction pathway may lead to altered gene expression to produce plants with desirable traits. In particular, manipulation of sugar signal transduction pathways could be used to alter source-sink relationships in seeds, tubers, roots and other storage organs leading to increase in yield.
Additionally, the morphological phenotypes shown by 35S::G916 seedlings indicate that the gene might be used to manipulate light responses such as shade avoidance.
G926 (SEQ ID NO: 155)
Published Information
G926 is equivalent to Hap2a (Y13720), a member of the CCAAT-box binding transcription factor family. The gene was identified by Edwards et al. ((1998) Plant Physiol. 117: 1015–1022). They showed that G926 or AtHap2a was able to functionally complement a Hap2 deficient mutant of yeast indicating that there is functional conservation between these proteins from diverse organisms. In addition, the AtHap2a gene was shown to be ubiquitously expressed in Arabidopsis . No functional information, however, was published for this gene.
Closely Related Genes from Other Species
G926 is most closely related to a Brassica napus protein (AAC49265). Similarity between the two proteins extends beyond the signature motif of the family to a level that would indicate the genes are orthologous. No functional information is available for the Brassica napus protein.
Experimental Observations
Consistent with the published expression pattern (Edwards et al. (1998) supra), G926 was determined to be ubiquitously expressed and transcript levels appeared to be unaltered by any environmental stress-related condition tested. A line homozygous for a T-DNA insertion in G926 was used to determine the function of this gene.
The G926 knockout mutant line was morphologically wild-type. Physiological analysis revealed that in the presumed absence of G926 function, the plants became more tolerant to high osmotic conditions during germination. This osmotic stress tolerance could be related to the plant's apparent insensitivity to the growth hormone ABA. This was the second instance where a member of a CCAAT-box protein complex altered the plants osmotic stress response and ABA sensitivity during germination. G926 and G11820 may function as part of the same complex or as part of the same or parallel signal transduction pathways.
G926 overexpressing plants were significantly greener and larger than wild-type control plants in a soil-based drought assay.
ABA plays an important regulatory role in the initiation and maintenance of seed dormancy. Lopez-Molina, L. et al. ((2001)) Proc. Natl. Acad. Sci. U.S.A. 98:4782–4787) described a bZIP transcription factor, ABI5, that is involved in maintaining seeds in a quiescent state, preventing germination under adverse conditions such as drought stress. It is possible G926 also functions as part of this checkpoint for the germinating seeds and loss of G926 function promotes germination regardless of the osmotic status of the environment.
Utilities
G926 or its equivalogs could be used to improve plant tolerance to drought and salt stress.
G961 (SEQ ID NO: 159)
Published Information
G961 was first identified in the sequence of the BAC clone F19D11, GenBank accession number AC005310, released by the Arabidopsis Genome Initiative.
Closely Related Genes from Other Species
A rice gene, GenBank accession number BAA84803, appears to be a gene that is related to G961.
Experimental Observations, Knockout Plants
The function of this gene was analyzed by knockout analysis. Homozygotes for a T-DNA insertion within G961 exhibited comparable morphology to wild type controls. However, these plants had altered seed oil content.
Experimental Observations, Overexpressors
Gene expression profiling by RT-PCR shows that G961 is primarily expressed in shoots, embryos and siliques at medium levels, and at low levels in flowers. RT-PCR data also indicates an induction of G961 transcript accumulation upon heat treatment.
35S::G961 Arabidopsis lines were generated, with which it was determined that overexpression of G961 produced marked changes in fertility and seed morphology. 35S::G961 transformants appeared wild-type at early stages of development, but following the switch to flowering, the majority of lines exhibited very poor fertility. Seeds from these plants frequently aborted and failed to mature. As a result of such deficiencies, the majority of the lines yielded very few seeds. The seeds that were obtained exhibited some striking differences in morphology compared to wild type controls; seed coloration was dark and white patches were visible on the seed coat, particularly at the tip near the micropyle. In some instances, it appeared as though the seeds might be germinating precociously. Aside from the poor germination efficiency observed for one of the G961 transgenic lines, no consistent differences were observed between G961 transgenics and the controls in the physiology assays.
Utilities
Based on the knock-out and overexpression phenotypes, G961 or its equivalogs might be used to manipulate oil and protein content of seeds. In particular, the changes in morphology and coloration shown by 35S::G961 seeds indicated that the gene or its equivalogs might be used to enhance seed traits or yield.
G975 (SEQ ID NO: 161)
Published Information
After its discovery by us, G975 has appeared in the sequences released by the Arabidopsis Genome Initiative (BAC F9L1, GenBank accession number AC007591).
Closely Related Genes from Other Species
The non-Arabidopsis gene most highly related to G975 (as detected in BLAST searches, Nov. 5, 1999) is represented by L46408 BNAF1258 Mustard flower buds Brassica rapa cDNA clone F1258. The similarity between G975 and the Brassica rapa gene represented by EST L46408 extends beyond the conserved AP2 domain that characterizes the AP2/EREBP family. In fact, this Brassica rapa gene appears to be more closely related to G975 than Arabidopsis G1387, indicating that EST L46408 may represent a true G975 ortholog. The similarity between G975 and Arabidopsis G1387 also extends beyond the conserved AP2 domain.
Experimental Observations
G975 was discovered by us and is a new member of the AP2/EREBP family (EREBP subfamily) of transcription factors. G975 is expressed in flowers and, at lower levels, in shoots, leaves, and siliques. GC-FID and GC-MS analyses of leaves from G975 overexpressing plants have shown that the levels of C29, C31, and C33 alkanes were substantially increased (up to 10-fold) compared to control plants. A number of additional compounds of similar molecular weight, presumably also wax components, also accumulated to significantly higher levels in G975 overexpressing plants. Although total amounts of wax in G975 overexpressing plants have not yet been measured, C29 alkanes constitute close to 50% of the wax content in wild-type plants (Millar et al. (1998) Plant Cell 11: 1889–1902), indicating that a major increase in total wax content occurs in these transgenic plants. However, the transgenic plants had an almost normal phenotype (small morphological differences are detected in leaf appearance), indicating that overexpression of G975 is not deleterious to the plant. It is noteworthy that overexpression of G975 did not cause the dramatic alterations in plant morphology that have been reported for Arabidopsis plants in which the FATTY ACID ELONGATION1 gene was overexpressed (Millar et al. (1998) supra). G975 could specifically regulate the expression of some of the genes involved in wax metabolism. One Arabidopsis AP2 gene was found that is significantly more closely related to G975 than the rest of the members of the AP2/EREBP family. This other gene, G1387, may have a function, and therefore a utility, related to that of G975.
Plants overexpressing G975 were significantly larger and greener than wild-type control plants in a soil-based drought assay.
Utilities
G975 or its equivalogs could be used to improve a plant's tolerance to drought or low water conditions.
G975 or its equivalogs could be used to manipulate wax composition, amount, or distribution, which in turn could modify plant tolerance to drought and/or low humidity or resistance to insects, as well as plant appearance (shiny leaves). A possible application for this gene or its equivalogs might be in reducing the wax coating on sunflower seeds (the wax fouls the oil extraction system during sunflower seed processing for oil). For this purpose, antisense or co-suppression of the gene in a tissue specific manner might be useful.
G975 could also be used to specifically alter wax composition, amount, or distribution in those plants and crops from which wax is a valuable product.
G101 (SEQ ID NO: 163)
Published Information
G1011 was identified in the sequence of P1 clone MTG10 (gene MTG10.20, GenBank accession number BAB10179.1). No information is available about the function(s) of G1011.
Experimental Observations
The complete cDNA sequence of G1011 was determined, and the initial BAC annotation in GenBank was found to be incorrect. The G1011 cDNA sequence has now been confirmed by a number of full-length cDNA sequences, which have recently been deposited in GenBank.
G1011 function was examined via analysis of a T-DNA insertion mutant for the gene. However, plants that were homozygous for this insertion displayed a wild-type phenotype in all assays performed. Additionally, RT-PCR studies on wild-type plants revealed G1011 expression to be ubiquitously expressed at low levels in a range of tissues.
We have now assessed the role of G1011 by analysis of transgenic Arabidopsis lines in which the gene was overexpressed. 35S::G10111 transformants appeared wild-type in the physiology assays, but did displayed a number of interesting developmental changes during the morphological assays. First, around half of the lines were markedly early flowering. Such effects were observed under either inductive (24-hour light) or non-inductive (12-hour light) photoperiodic conditions, indicating that G1011 might have a central role in determining the timing of the floral transition. Interestingly, under 12-hour light conditions, the lines also developed shorter, more rounded leaves than wild type, but this was not seen under continuous light.
As well as the effects on flowering time, many of the 35S::G1011 lines displayed alterations in flower morphology; floral organs often had alterations in shape or number and petals were rather narrow and green. In particular, it was noted that floral organ abscission was somewhat delayed compared to wild-type flowers, with stamens, petals, and sepals persisting following pollination. It is noteworthy that Ferrandiz et al. ((2000) Plant Cell 12, 183–198) reported similar phenotypes as a result of overexpression of another MADS gene, AGL15.
Utilities
Based on the phenotypes observed in morphological assays, G1011 could have a number of applications.
Given its effects on the floral transition, G1011 might be used to manipulate the flowering time of commercial species. In particular, the gene could be use to accelerate flowering or to eliminate any requirement for vernalization.
The effects on flower morphology are also of commercial interest. G1011 might be used to modify flower development, in order to change form of flowers and fruits. This could create attractive new varieties or be used to influence pollination efficiency. The persistence of outer whorl organs following pollination is also of interest; such a trait could be applied to ornamental plants to prolong the life of blooms.
G1013 (SEQ ID NO: 165)
Published Information
G1013 (At5g43290) is a novel member of the WRKY family of transcription factors. No information is available about the function(s) of G1013.
Experimental Observations
RT-PCR analysis was used to look at the endogenous expression of G1013. Expression of the gene was only detected in floral tissues. It does not appear to be induced by any of the conditions tested.
Homozygous plants were analyzed for a T-DNA insertion within G1013 and found that they showed wild-type morphology at all developmental stages.
The effects of G1013 overexpression were studied. In an assay intended to determine whether the transgene expression could alter C:N sensing, 35S::G1013 seedlings contained less anthocyanins than wild-type controls grown on high sucrose/N-plates. Seedlings were also greener than the wild-type controls on high sucrose/N-/Gln plates. These data together indicate that overexpression of G1013 alters a plant's ability to modulate carbon and/or nitrogen uptake and utilization.
G1013 overexpression also had an effect on plant morphology. 35S::G1013 lines exhibited narrow downward curled leaves, which were sometimes held in a more upright orientation than those of wild type at early stages of growth. Plants from the two T2 lines grown under continuous light also flowered late. In addition to the effects on leaf shape, many lines were slightly smaller than controls, and a few showed sporadic defects in flower development.
Utilities
On the basis of the available analytical data, there are several potential applications for G1013:
(1) the gene or its orthologs could be used to alter plant leaf morphology;
(2) the observation that 35S::G1013 lines make less anthocyanin on high sucrose plus glutamine, indicated G1013 or its orthologs might be used to modify carbon and nitrogen status, and hence assimilate partitioning. The enhanced performance of G1013 overexpression lines under low nitrogen conditions indicate that the gene could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
(3) the promoter of G1013 could be used to drive floral specific expression in planta.
G1037 (SEQ ID NO: 171 and SEQ ID NO: 2108)
Published Information
G1037 was identified in the sequence of BAC MUJ8, GenBank accession number AB028621, released by the Arabidopsis Genome Initiative. G1037 has been named ARR12 (Hwang et al. (2002) Plant Physiol. 129: 500–515). G1037 was identified in the sequence of BAC F13D4, GenBank accession number AL031369, released by the Arabidopsis Genome Initiative. This BAC has since been removed from GenBank, and currently the genomic sequence is not present. G1037 corresponds to the TAIR locus AT2G25180. It is cited in the patent publication WO0216655 concerning stress-regulated genes (Harper et al. (2002)).
Closely Related Genes from Other Species
Several genes with strong similarity to G1037 are present in other species. The most closely related are a putative response regulator from maize (AB062095) and a Brassica oleracea gene represented by genomic clone BH007675. No further information is available about these genes.
Experimental Observations
G1037 is a member of the response regulator class of GARP proteins. G1037 was found to be expressed throughout the plant, with highest expression in roots. It may be induced by auxin, ABA, heat, salt, and salicylic acid treatments.
A line homozygous for a T-DNA insertion in G1037 was used to determine the function of this gene. The T-DNA insertion of G1037 was determined to be approximately one third of the way into the coding sequence of the gene, within the conserved GARP domain, and therefore was likely to result in a null mutation. Plants homozygous for the T-DNA insertion showed somewhat inconsistent changes in flowering time. In one experiment, two different populations of G1037 knockout plants were markedly early flowering. However, in a second experiment, only a proportion of the plants showed early flowering, and this phenotype was marginal. It is possible that the effects of a G1037 mutation on flowering time are dependent on environmental conditions. No altered phenotypes of G1037 knockout plants were detected in any of the physiological or biochemical assays.
The function of this gene has also been analyzed using transgenic plants in which G1037 was expressed under the control of the 35S promoter. It should be noted that the clone contained sequence differences (SEQ ID NO: 2108) from the public BAC sequence (SEQ ID NO: 171). Two 35S::G1037 lines showed more tolerance to salt stress in a germination assay. All 35S::G1037 lines showed wild-type morphology.
Because several members of the response regulator class of GARP genes have been implicated in cytokinin signaling, it is possible that the improved seedling growth noted on salt results from changes in hormone response pathways.
Utilities
G1037 or its equivalogs may be useful for alteration of flowering time in crop plants.
G1037 or its equivalogs may be useful for engineering salt tolerance. The salt tolerance of G1037 seedlings may also indicate a general increase in tolerance to osmotic stress, indicating a potential use for G1037 or its equivalogs in engineering drought tolerance.
G1128 (SEQ ID NO: 181)
Published Information
The sequence of G1128 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AB018109, based on its sequence similarity within the conserved domain to other AT-Hook related proteins in Arabidopsis.
Experimental Observations
Gene expression profiling using RT/PCR shows that G1128 is predominantly expressed in roots and flowers. Its expression appears to be not induced by any treatments tested.
Previously, the function of this gene was studied by knockout analysis. Plants homozygous for a T-DNA insertion in G1128 were wild type for all assays performed. It should be pointed out that the functional knockout analysis for AT-Hook proteins has not provided useful information so far, as was the case for G280 and G1945. One of reasons could be that there is functional redundancy among some of AT-Hook proteins. In fact, G1128 protein shares a significant homology to one other AT-Hook protein G1399.
The function of G1128 was also studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1128 in Arabidopsis produced a wide range of morphological changes including stunted growth, and alterations in leaf and flower development. Analysis of G1128 overexpressors reveals no apparent physiological changes when compared to wild-type control plants.
Utilities
Based on the effects of G1128 overexpression, the gene could be used to manipulate plant growth and development. In particular, the accelerated senescence of the 35S::G1128 lines indicates that the gene could be used to modify disease responses, or alter the rate of senescence in crops. Additionally, if the dark coloration of 35S::G1128 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G1142 (SEQ ID NO: 185)
Published Information
The sequence of G1142 was obtained from the Arabidopsis genome sequencing project (within clone T3K9, GenBank accession number AC004261), based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
RT-PCR analysis indicated that G1142 is ubiquitously expressed. The function of this gene was first studied by knockout analysis. Homozygous plants carrying a T-DNA insertion in G1142 flowered slightly earlier than wild-type controls under continuous conditions light. This phenotype was observed in two independently grown populations of KO.G1142 plants. G1142 knock-out plants were otherwise identical to their wild-type counterparts in all physiological and biochemical assays.
The function of G1142 has now been assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from the 35S CaMV promoter. Under continuous light, 35S::G1142 transformants displayed narrow leaves and flowered approximately 5–7 days later than wild-type controls. However, G1142 overexpressing lines behaved similarly to the wild-type controls in all physiological assays performed.
Utilities
Based on the analysis of G1142 knock-out plants as well as 35S::G1142 transgenic lines, G1142 or its orthologs can be used to manipulate flowering time in commercial species.
The delayed flowering displayed by 35S::G1142 transformants indicated that the gene or its orthologs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Given the early flowering seen in the G1142 null mutant, it is likely that the activity of G1142 or its orthologs can accelerate flowering or eliminate any requirement for vernalization.
The changes in leaf shape in 35S::G1142 lines also indicate that the gene or its orthologs can be used to modify plant architecture.
G1206 (SEQ ID NO: 189)
Published Information
G1206 was identified by amino acid sequence similarity to the pea early nodulin gene-binding protein 1 (ENBP1), which binds to an AT-rich sequence motif within the promoter of the early nodulin gene ENOD12 (Christiansen et al., (1996) Plant Mol. Biol. 32:809–821). G1206 is in chromosome 1, BAC F24O1 (GenBank accession AC003113.2 GI:7658296), released by the Arabidopsis Genome Initiative. The translational start and stop codons were correctly predicted. No public information related to the functional characterization of G1206 has been published or made available.
Experimental Observations
An analysis of the endogenous levels of G1206 transcripts by RT-PCR revealed a constitutive expression in all tissues tested. No change in G1206 expression was observed in the biotic and abiotic treatments examined. A line homozygous for a T-DNA insertion in G1206 was used to determine the function of this gene. The characterization of the G1206 null mutant showed no apparent morphological, physiological or biochemical changes when compared to control plants.
The function of G1206 was also analyzed through its ectopic overexpression in plants. Physiological analysis of 35S::G1206 overexpressor lines revealed increased seedling vigor under drought conditions. Seedlings were generally larger and greener than the control plants treated with the same conditions.
Utilities
The reduced sensitivity of 35S::G1206 lines in the dehydration stress assay indicates that the gene might be used to engineer crops with increased tolerance to drought, salt, freezing and chilling stress, or increased water use efficiency.
G1274 (SEQ ID NO: 193)
Published Information
G1274 is a member of the WRKY family of transcription factors. The gene corresponds to WRKY51 (At5g64810). No information is available about the function(s) of G1274.
Experimental Observations
RT-PCR analysis was used to determine the endogenous expression pattern of G1274. Expression of G1274 was detected in leaf, root and flower tissues. The biotic stress related conditions, Erysiphe and SA induced expression of G1274 in leaf tissue. The gene also appeared to be slightly induced by osmotic and cold stress treatments and perhaps by auxin.
The function of G1274 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. G1274 overexpressing lines were more tolerant to growth on low nitrogen containing media. In an assay intended to determine whether the transgene expression could alter C/N sensing, 35S::G1274 seedlings contained less anthocyanins ( FIG. 5A ) than wild-type controls ( FIG. 5B ) grown on high sucrose/N- and high sucrose/N/Gln plates. These data together indicated that overexpression of G1274 may alter a plant's ability to modulate carbon and/or nitrogen uptake and utilization.
G1274 overexpression and wild-type germination were also compared in a cold germination assay, the overexpressors appearing larger and greener ( FIG. 5C ) than the controls ( FIG. 5D ).
FIGS. 6A–6D compare soil-based drought assays for G1274 overexpressors and wild-type control plants, which confirms the results predicted after the performance of the plate-based osmotic stress assays. 35S::G1274 lines fared much better after a period of water deprivation ( FIG. 6A ) than control plants in ( FIG. 6B ). This distinction was particularly evident in the overexpressor plants after once again being watered, said plants almost all fully recovered to a healthy and vigorous state in FIG. 6C . Conversely, none of the wild-type plants seen in FIG. 6D recovered after rewatering, as it was apparently too late for rehydration to rescue these plants.
In addition, 35S::G1274 transgenic plants were more tolerant to chilling compared to the wild-type controls, in both germination as well as seedling growth assays. 35S::G1274 overexpression plants were significantly greener and larger than wild-type control plants in a soil-based drought assay.
Overexpression of G1274 produced alterations in leaf morphology and inflorescence architecture. Four out of eighteen 35S::G1274 primary transformants were slightly small and developed inflorescences that were short, and showed reduced internode elongation, leading to a bushier, more compact stature than in wild-type.
In an experiment using T2 populations, it was observed that the rosette leaves from many of the plants were distinctly broad and appeared to have a greater rosette biomass than in wild type.
A similar inflorescence phenotype was obtained from overexpression of a potentially related WRKY gene, G1275. However, G1275 also caused extreme dwarfing, which was not apparent when G1274 was overexpressed.
Utilities
The phenotypic effects of G1274 overexpression could have several potential applications:
The enhanced performance of 35S::G1274 plants in a soil-based drought assay indicated that the gene or its equivalogs may be used to enhance drought tolerance in plants.
The enhanced performance of 35S::G1274 seedlings under chilling conditions indicates that the gene or its equivalogs might be applied to engineer crops that show better growth under cold conditions.
The morphological phenotype shown by 35S::G1274 lines indicate that the gene or its equivalogs might be used to alter inflorescence architecture, to produce more compact dwarf forms that might afford yield benefits.
The effects on leaf size that were observed as a result of G1274 or equivalog overexpression might also have commercial applications. Increased leaf size, or an extended period of leaf growth, could increase photosynthetic capacity, and biomass, and have a positive effect on yield.
G1276 (SEQ ID NO: 195)
Published Information
G1276 (At5g60120) was identified as part of P1 clone: MGO3 (GenBank accession AB019231).
Experimental Observations
G1276 was found to be expressed ubiquitously in Arabidopsis . The function of this gene was analyzed using transgenic plants in which a G1276 cDNA clone was expressed under the control of the 35S promoter. Overexpression of G1276 in Arabidopsis delayed the onset of flowering by up to 2–3 weeks under continuous light conditions. No consistent differences were observed between the 35S::G1276 transgenics and the wild-type control plants in any of the physiology assays.
It is noteworthy that G1276 is a potential paralog of APETALA2 (G2) and that a number of genes from the G2 clade produced delayed flowering when overexpressed.
Utilities
The delayed flowering displayed by 35S::G1276 transformants indicated that the gene or its equivalogs might be used to manipulate the flowering time of commercial species. Given the effects of G1276 overexpression, it is likely that the activity of the gene or its equivalogs could be modified to accelerate flowering, or eliminate any requirement for vernalization.
G1313 (SEQ ID NO: 199)
Published Information
G1313 (At5g06100) corresponds to AtMYB33. Gocal et al. ((2001) Plant Physiol. 127: 1682–1693) showed that G11313 (AtMYB33) could bind to the GA (gibberellin) response element and activate the barley alpha-amylase promoter in a transient assay in barley aleurone cells. The gene was ubiquitously expressed in Arabidopsis . It was hypothesized that the gene could regulate GA responsive pathways that promote flowering in Arabidopsis . To test this hypothesis, Gocal et al. (supra) analyzed that whether AtMYB33 was capable of binding to the LFY gene promoter. LFY is a floral meristem identity gene that has a GA responsive element in its promoter. AtMYB33 was found to bind to the LFY promoter suggesting that the action of gibberellins on flowering could be mediated through the activity of AtMYB33 (Gocal et al. supra).
Experimental Observations
The complete sequence of G1313 was determined. The function of this gene was analyzed using transgenic plants in which G1313 was expressed under the control of the 35S promoter. 35S::G1313 transgenics were wild-type in response to all physiological stress treatments performed.
Overexpression of G1313 produced an increase in seedling vigor in some of the T1 plants at an early seedling stage under normal growth conditions compared to the wild-type controls; transgenic Arabidopsis seedlings were up to two-fold larger than the wild-type seedlings at early stages of development. Given that gibberellins are known to promote seed germination, the increased seedling vigor may be related to a GA response in seeds. The lack on an effect of G1313 on flowering time may result from the fact that an additional factor is required for the activity of the protein. All assays were performed under continuous light.
Utilities
The increase in seedling vigor in G1313 transgenics plants indicated this gene or its orthologs could be used to increased survivability and vigor of small seedlings under field conditions potentially leading to a greater yield in crops. Published results indicate that the gene might modify a plant's response to the growth regulator gibberellic acid (Gocal et al. supra).
G1357 (SEQ ID NO: 207)
Published Information
G1357 corresponds to gene At3g44290, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G1357.
Experimental Observations
The complete sequence of G1357 was experimentally determined. G1357 expression was not detected in wild-type plants under our experimental conditions. The function of this gene was analyzed using transgenic plants in which G1357 was expressed under the control of the 35S promoter.
35S::G1357 seedlings were more tolerant to chilling stress in a growth assay and insensitive to ABA in a germination assay. Morphologically, overexpression of G1357 in Arabidopsis produced alterations in coloration, leaf shape, and a marked delay in the time to flowering. At the earliest stages, G1357 seedlings appeared normal, but towards the mid-rosette stage, the plants developed a darker green coloration and the leaves became slightly rounder than those of wild-type. Additionally, many lines were also slightly smaller than controls. The majority of lines produced flower buds markedly late, with the most severely affected individuals flowering approximately 1 month later than wild type under continuous light conditions.
In a soil based drought assay, G1357 overexpressing plants were significantly greener and larger than wild-type control plants.
It should be noted that a highly related gene, G1452 (analyzed in phase I) had similar endogenous expression patterns, and produced similar effects on coloration, leaf shape, flowering time, abiotic stress resistance, and ABA sensitivity.
Utilities
The results of physiological assays indicated that G1357 gene or its equivalogs could be used to improve a plant's tolerance to chilling stress and drought.
Enhanced chilling tolerance could also extend the effective growth range of chilling sensitive crop species by allowing earlier planting or later harvest.
The delayed flowering displayed by 35S::G1357 transformants indicated that the gene or its equivalogs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Given the effects of G1357 overexpression, it is likely that the activity of the gene or its equivalogs could be modified to accelerate flowering, or eliminate any requirement for vernalization.
Additionally, if the dark coloration of 35S::G1357 lines reflects an increase in biochemical composition, this gene or its equivalogs might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G1412 (SEQ ID NO: 215)
Published Information
G1412 is a member of the NAC family of transcription factors. G1412 was identified in the sequence of BAC clone F27G19, GenBank accession number AL078467, released by the Arabidopsis Genome Initiative. G1412 also corresponds to gene At4g27410, annotated by the Arabidopsis Genome Initiative, and to sequence 1543 from patent publication WO0216655 A2 on stress-regulated genes, transgenic plants and methods of use. In the latter publication, G1412 was reported to be cold, osmotic and salt responsive in microarray analysis. No information is available about the function(s) of G1412.
Closely Related Genes from Other Species
G1412 is very similar in sequence to LEJA2 from tomato that is regulated by jasmonic acid. The level of sequence homology between these two proteins is significant enough to indicate they could have similar functions in the plant.
Experimental Observations
RT-PCR was used to analyze the endogenous expression pattern of G1412. G1412 appears to be constitutively expressed in all tissues tested. G1412 induction was observed in response to ABA, heat, drought, mannitol and Erysiphe , indicating the gene's expression is regulated by environmental conditions.
A T-DNA insertion mutant for G1412 was analyzed. The mutant displayed a wild-type morphology, and was wild-type in its response to the physiological analyses that were performed.
The effects of G1412 overexpression were also studied; the transformants displayed wild-type morphology. However, the 35S::G1412 transgenics were insensitive to ABA and were more tolerant to osmotic stress in a germination assay on media containing high concentrations of sucrose.
Utilities
The phenotypic effects of G1412 overexpression, such as the increase in seedling vigor observed in a germination assay on high sucrose media and insensitivity to germination on ABA media, indicated that the gene or its equivalogs could be used to engineer plants with increased tolerance to abiotic stresses such as drought, salt, heat or cold.
G1420 (SEQ ID NO: 217)
Published Information
G1420 corresponds to gene AT5g49520, and it has also been described as WRKY48. No information is available about the function(s) of G1420.
Experimental Observations
G1420 is ubiquitously expressed in Arabidopsis and does not appear to be significantly induced by any of the conditions tested.
A T-DNA insertion mutant for G1420 was analyzed, and the mutant was phenotypically wild-type.
We have now generated 35S::G1420 lines. Overexpression of the gene in Arabidopsis produced marked alterations in the morphology of leaves and floral organs. 35S::G1420 seedlings typically displayed rather long narrow cotyledons. Later, the plants formed leaves that were often mildly serrated, narrow, slightly dark green, and rather contorted. Additionally many of the lines showed stunted growth and appeared markedly smaller than controls. Following the switch to reproductive growth, 35S::G1420 transformants developed rather thin spindly inflorescences. Flowers were often borne on particularly long pedicels, and floral organs, especially sepals and petals, were long, narrow and twisted in a comparable manner to the leaves. As a result of the reduced size, and floral abnormalities, the seed yield from most of the lines was very poor.
In addition to the developmental alterations produced as a consequence of G1420 overexpression, the 35S::G1420 seedlings displayed a sugar sensing phenotype in a germination assay on media containing high glucose.
Utilities
The results of physiological assays indicate that G1420 could be used to alter the sugar signaling in plants.
The effects of G1420 on plant development indicate that the gene could be used to manipulate architecture. In particular, the gene could be used to generate novel leaf and flower forms for the ornamental markets. Additionally, if the dark coloration of 35S::G1420 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G1451 (SEQ ID NO: 223)
Published Information
G1451 is ARF8, a member of the ARF class of proteins with a VP1-like N-terminal domain and a C-terminal domain with homology to Aux/IAA proteins. ARF8, like several other ARFs, contains a glutamine-rich central domain that can function as a transcriptional activation domain (Ulmasov et al. (1999a) Proc. Natl. Acad. Sci. 96: 5844–5849). ARF8 was shown to bind to an auxin response element (Ulmasov et al. (1999b) Plant J. 19: 309–319). It was also shown that a truncated version of ARF8 lacking the DNA binding domain but containing the activation domain and the C-terminal domain could activate transcription on an auxin responsive promoter, presumably through interactions with another factor bound to the auxin response element (Ulmasov et al. 1999a, supra). ARF8 is closely related in sequence to ARF6 (Ulmasov et al. 1999b, supra).
Experimental Observations
A line homozygous for a T-DNA insertion in G1451 exhibited a change in seed oil content. RT-PCR studies revealed that G11451 was expressed throughout the plant, with the highest expression in flowers. Transcripts of G1451 were induced in leaves by a variety of stress conditions.
The function of G1451 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1451 produced changes in leaf morphology and a general increase in the vegetative biomass of Arabidopsis plants. At early stages, 35S::G1451 transformants appeared normal. However, towards the end of the rosette phase, leaves became distinctly broader and longer than wild type leaves. Many of the plants showing this phenotype also exhibited a mild delay in the onset of flowering.
Utilities
G1451 or its orthologs can be used to increase plant biomass, thus improving yield. Additionally, the delay in flowering observed in some of the 35S::G1451 lines indicated that the gene might be used to manipulate the timing of reproductive growth.
G1452 (SEQ ID NO: 225)
Published Information
G1452 was identified in the sequence of clones T22O13, F12K2 with accession number AC006233 released by the Arabidopsis Genome Initiative. No information is available about the function(s) of G1452.
Experimental Observations
The function of G1452 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1452 produced changes in leaf development and markedly delayed the onset of flowering. 35S::G1452 plants produced dark green, flat, rounded leaves, and typically formed flower buds between 2 and 14 days later than controls. Additionally, some of the transformants were noted to have low trichome density on leaves and stems. At later stages of life cycle, 35S::G1452 plants developed more slowly and senesced considerably later than wild-type controls. In addition, G1452 overexpressors were more tolerant to osmotic stress, and were insensitive to ABA in separate germination assays.
G1452 expression was not detected in any tissue tested by RT-PCR and was not induced by any environmental stress-related condition tested.
Utilities
On the basis of the analyses performed to date, G1452 or its equivalogs could be use to alter plant growth and development. In addition, G1452 or its equivalogs could be used to alter a plant's response to water deficit conditions and therefore, could be used to engineer plants with enhanced tolerance to drought and salt stress.
G1468 (SEQ ID NO: 227)
Published Information
The genomic sequence of G1468 is located on the Arabidopsis BAC clone T7123 (GenBank accession number U89959).
Experimental Observations
G1468 was predominantly expressed in flowers and embryos.
A line homozygous for a T-DNA insertion in G1468 was used to determine the function of this gene. The T-DNA insertion of G1468 was found to be within the first third of the coding sequence of the gene and therefore was likely to result in a null mutation. Furthermore, its expression level was unaffected by any of the conditions tested. G1468 knockout mutant plants behaved similarly to wild-type plants in all assays performed.
The function of G1468 was also studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1468 produced plants that were very tiny and rather dark in coloration compared to wild type controls at early stages. Severely affected individuals arrested growth early in vegetative development. Plants that survived formed narrow, gray leaves and showed a marked delay in the onset of flowering. Many of the late flowering plants had more axillary rosette leaves compared to controls leading to an increase in vegetative biomass.
Utilities
The alterations in leaf shape, size, and coloration shown by 35S::G1468 transformants indicated that the gene or its equivalogs might be applied to modify plant architecture.
The delayed bolting indicated that gene or its equivalogs might also be used to manipulate flowering time in commercial species. Conversely, it is possible that the activity of G1468 or its equivalogs could be modified to accelerate flowering, or eliminate any requirement for vernalization.
G1476 (SEQ ID NO: 231)
Published Information
G1476 (At5g43540) was identified in the sequence of TAC clone K9D7 (GenBank accession number AB016875) based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about the function of G1476.
Experimental Observations
G1476 is expressed in roots, flowers, embryos and germinating seeds.
The function of G1476 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1476 produced highly deleterious effects on growth and development. At early stages, while on media, 35S::G1476 seedlings appeared to grow more rapidly than controls.
Utilities
Based on the effects of its overexpression, G1476 could be used to regulate plant growth and development.
G1482 (SEQ ID NO: 233)
Published Information
G1482 was identified as a gene in the sequence of BAC F10A5, GenBank accession number AC006434, released by the Arabidopsis Genome Initiative. There is no other published or public information about G1482.
Experimental Observations
The sequence of G1482 was experimentally determined. Homozygous plants harboring a T-DNA insertion in G1482 displayed significantly more root growth on MS control plates as well as on different stresses in three separate experiments.
The function of G1482 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter G1482 overexpression in Arabidopsis resulted in visually high levels of the anthocyanin pigment production throughout the plant.
Utilities
Based on the phenotypes produced when this gene is knocked out, G1482 or its orthologs can be used to manipulate root growth, particularly in response to environmental stresses such as drought and low nutrients.
In addition, G1482 or its orthologs could be used to modulate anthocyanin levels. The potential utilities of genes involved in anthocyanin production include alterations in pigment production for horticultural purposes and increase stress resistance perhaps in combination with other transcription factors. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. In addition, several flavonoid compounds have health promoting effects such as the inhibition of tumor growth and cancer, prevention of bone loss and the prevention of the oxidation of lipids. Given that the phenylpropanoid biosynthetic pathway (from which anthocyanins are produced) feeds into the pathways for the production of a number of other classes of secondary metabolites, such as lignins and tannins, changing the activity of G1482 or its orthologs might also influence the levels of those types of compounds.
G1510 (SEQ ID NO: 241)
Published Information
G1510 was identified in the sequence of P1 clone MPI10, GenBank accession number AB020747, released by the Arabidopsis Genome Initiative. There is no other published or public information about G1510.
Experimental Observations
The 5′ and 3′ ends of G1510 were experimentally determined by RACE. RT-PCR expression analysis showed that G1510 is expressed in all tissues except roots, suggesting that the gene could have a role within green tissues.
The function of this gene was analyzed using transgenic plants in which G1510 was expressed under the control of the 35S promoter. 35S::G1510 plants showed a dramatic change in coloration and were much darker green compared to controls. Green pigmentation also extended into the hypocotyls and roots from these plants, suggesting that the native function of G1510 could be related to plastid differentiation, chlorophyll production, or the regulation of chloroplast number. 35S::G1510 also exhibited disproportionately long hypocotyls, indicating that the gene could influence light-regulated developmental processes.
Utilities
The increased pigmentation indicated that 35S::G1510 plants had altered levels of chlorophylls or carotenoids. As such the gene or its orthologs could have a number of valuable applications.
Enhanced chlorophyll and carotenoid levels could improve yield and nutritional value in crop plants. For instance lutein, like other xanthophylls such as zeaxanthin and violaxanthin, is an essential component in the protection of the plant against the damaging effects of excessive light. Specifically, lutein contributes to the rapid rise of non-photochemical quenching in plants exposed to high light. Crop plants engineered to contain higher levels of lutein could therefore have improved photo-protection, possibly leading to less oxidative damage and better growth under high light. Additionally, elevated chlorophyll levels might increase photosynthetic capacity, and hence yield.
G1510 or its orthologs might be also applied to improve the nutraceutical value of foodstuffs. For example, consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of age-related macular degeneration (ARMD), the leading cause of blindness in elderly people.
G1538 (SEQ ID NO: 245)
Published Information
G1538 encodes a HD-ZIP class I homeodomain protein and corresponds to gene MSN2.9 within P1 clone MSN2 (chromosome 5, GenBank Accession AB018119). No published data are available pertaining to the function of this gene.
Experimental Observations
G1538 function was examined via analysis of a T-DNA insertion mutant for the gene. However, plants that were homozygous for this insertion displayed a wild-type phenotype in all assays performed. Nevertheless, RT-PCR studies on wild-type plants revealed G1538 expression to be induced in leaves by heat and salicylic acid treatments. Under normal physiological conditions, G1538 was expressed at moderately high levels in roots, flowers and siliques, but at rather low levels in leaves, shoot stems, embryos, and germinating seeds.
We have now assessed the role of G1538 by analysis of transgenic Arabidopsis lines in which the gene was overexpressed. The boundaries of G1538 were identified by RACE experiments, a clone was amplified from cDNA derived from mixed tissues, and 35S::G1538 lines were generated. Approximately half of the T1 lines flowered earlier than wild-type controls under continuous light conditions, but this phenotype was not apparent in three of those lines, which were grown under a less inductive 12-hour photoperiod in the T2 generation. Interestingly, though, the plants from all three T2 lines did develop slightly longer leaf petioles than wild type. Such an effect had not been noted among the primary transformants, but it is noteworthy that increased petiole length is sometimes associated with accelerated or delayed flowering.
Given the alterations in flowering time, it is possible, that elevated G1538 activity can accelerate flowering specifically under inductive photoperiodic conditions. However, such effects on flowering time should be further examined by growing larger populations of plants from a number of different lines, under a variety of growth conditions.
Importantly, 35S::G1538 transformants also displayed a pronounced phenotype in the physiological assays: each of three independent T2 lines had improved tolerance to salt stress in a plate-based root growth assay. The 35S::G1538 seedlings were larger and displayed more secondary root growth than wild-type controls subjected to the same treatments.
Utilities
Based on the phenotypes observed in morphological and physiological assays, G1538 might be have a number of utilities.
Given the salt resistance exhibited by 35S::G1538 transformants, the gene might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions.
The early flowering displayed by 35S::G1538 transformants indicates that the gene might be used to accelerate the flowering of commercial species, or to eliminate any requirements for vernalization.
Finally, as noted in our earlier reports, the RT-PCR experiments indicate that the gene and/or its promoter could be useful in designing plants that are more resilient to heat or physiological conditions that result in high levels of salicylic acid. The G1538 promoter might also be applied to create gene expression systems that are heat or salicylic acid inducible.
G1539 (SEQ ID NO: 247)
Published Information
G1539 was identified within a sequence released by the Arabidopsis genome initiative (gene MEB5.20, P1 clone MEB5, Chromosome 3, GenBank accession, AB019230,), as a gene encoding a novel WUSCHEL-like homeodomain protein. No data regarding the function of this gene are available in the public literature.
Experimental Observations
The boundaries of G1539 were determined by RACE experiments, and transgenic lines were generated in which the gene was overexpressed from a 35S promoter. These plants displayed a wild-type response in all of the physiological assays, but showed some striking alterations in morphology compared to controls. 35S::G1539 lines exhibited a spectrum of developmental changes including alterations in leaf shape, phyllotaxy, coloration, growth rate, floral organ abnormalities, and a reduction in overall size. However, the most prominent phenotype was seen in the inflorescence, where strange growths, which took on a carpelloid identity, developed from stems, pedicels and floral organs. Occasionally, on the stems of 35S::G1539 T1 plants, trichomes were positioned at the apex of gland-like structures.
Similar results were previously obtained from overexpression of a related gene, WUSCHEL (G1540), which was found to induce the formation of callus like tissue that later took on a carpelloid identity. WUSCHEL has a key role in the maintenance of stem cell identity within apical meristems, and during the reproductive phase, participates in a feedback loop with the AGAMOUS gene, which induces floral meristems to terminally differentiate into carpels (Mayer et al. (1998) Cell 95: 805–815; Schoof et al (2000) Cell 100: 635–644; Lohmann et al. (2001) Cell 105: 793–803). The similarity between the WUS and G1539 overexpression phenotypes indicated that the genes have similar roles in regulating apical meristem activity.
Two other WUS-like genes, G1591 and G2983, have also yielded similar overexpression phenotypes to G1539.
Utilities
Given its capacity to trigger ectopic carpel development in Arabidopsis , G1539 or its orthologs could be applied to commercial species to induce formation of increased numbers of carpels or fruits. A particular application might exist in saffron, one of the world's most expensive spices. Saffron filaments, or threads, are actually the dried stigmas of the saffron flower, Crocus Sativus Linneaus. Each flower contains only three stigmas, and more than 75,000 of these flowers are needed to produce just one pound of saffron filaments. A gene such as G1539, which increased carpel numbers, could therefore substantially increase yield.
Additionally, the overexpression phenotypes of G1539 indicate that it or its orthologs might be used to regulate meristem activity and stem cell identity. As such, the gene could have applications in the plant cell culture lines, or in transformation or micro-propagation systems, where generation of callus is currently problematic but is required as part of the procedure.
The alterations in trichome development seen in occasional lines indicated that the gene or its orthologs could be used to manipulate the formation of those structures.
G1557 (SEQ ID NO: 255)
Published Information
G1557 was identified in the sequence of chromosome 4, GenBank accession number AL161501, released by the Arabidopsis Genome Initiative. It is not annotated in the public sequence. No functional information is available about G1557.
Experimental Observations
The function of G1557 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. G1557 overexpression in Arabidopsis resulted in increased seedling vigor in response to salt stress in a germination assay.
Utilities
G1557 or its orthologs may be useful for increasing salt tolerance. Salt (and drought) stress signal transduction consists of ionic and osmotic homeostasis signaling pathways. The pathway regulating ion homeostasis in response to salt stress has been reviewed recently by Xiong and Zhu ((2002) Plant Cell Environ. 25: 131–139). The osmotic component of salt stress involves complex plant reactions that are possibly overlapping with drought and/or cold stress responses. Common aspects of drought, cold and salt stress response have been reviewed recently by Xiong et al. (Xiong et al. (2002) Plant Cell 14 Suppl. S165–S183).
G1593 (SEQ ID NO: 261)
Published Information
G1593 was initially identified within a sequence released by the Arabidopsis genome initiative (gene T22O13.1 within clone T22O13, chromosome 2, GenBank accession, AC007290), as a gene encoding a novel homeodomain protein of the BEL1 class. The gene has been designated AGI number At2g27220, but no public data are available regarding its function.
Experimental Observations
The boundaries of G1593 were determined by RACE experiments, and transgenic lines were generated in which the gene was overexpressed from a 35S promoter. These transformants exhibited a wild-type response to physiological assays, but displayed a number of morphological phenotypes. 35S::G1593 lines were dark in coloration, displayed alterations in leaf shape, and formed shorter, more compact inflorescences than controls.
Utilities
The changes in morphology shown by the 35S::G1593 transformants indicate that the gene or its orthologs could be used to manipulate inflorescence architecture and branching patterns in commercial species, to create varieties with more compact forms. In particular, dwarf and compact forms of ornamental plants are extremely popular among consumers. They represent a lucrative market for breeders and growers alike, but currently for many varieties, suitable dwarf breeding lines are either unavailable or difficult to integrate into existing germ-lines. Therefore, currently, many ornamental plants are sprayed with expensive chemical growth regulators to reduce height and increase compactness. Overexpression of a gene with G1593 activity could potentially alleviate this requirement.
Additionally, if the altered coloration of 35S::G1593 plants reflects a change in biochemical composition, the gene or its orthologs might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield. For example, consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of age-related macular degeneration (ARMD), the leading cause of blindness in elderly people.
G1660 (SEQ ID NO: 263)
Published Information
G1660 was identified by amino acid sequence similarity to other DNA-binding proteins. G1660 is found in the sequence of the chromosome 2 BAC clone F504 (GenBank accession number AC005936, nid=g4038029), released by the Arabidopsis Genome Initiative. No information related to the functional characterization of G1660 is currently available from the public literature.
Experimental Observations
The 5′ and 3′ ends of G1660 were experimentally determined by RACE. The function of G1660 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Plants overexpressing G1660 had more root growth and seedling vigor when grown on media containing high salt, compared to wild-type control plants. Morphological analysis of transgenic plants revealed no phenotypic alterations.
Utilities
Based on the increased salt tolerance exhibited by the 35S::G1660 lines in physiology assays, this gene might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
G1730 (SEQ ID NO: 267)
Published Information
G1730 was identified in the sequence of BAC T32F12, GenBank accession number AC005314, released by the Arabidopsis Genome Initiative. There is no other published or public information about G1730.
Experimental Observations
The full-length cDNA clone corresponding to G1730 was isolated from a gene library. Based on RT-PCR experiments, G1730 was highly expressed in all tissues except roots, but was markedly repressed in rosette leaves by cold or osmotic stress.
The function of G1730 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G1730 plants showed wild-type morphology but displayed an enhanced performance compared to controls when subjected to osmotic stress in both mannitol and glucose germination assays. Given the expression profiles of the endogenous gene, and the putative role of RING C3H2C3 proteins in regulation of ubiquitin-dependent protein turnover, G11730 may act as a modulator of factors involved in the response to abiotic stress.
Utilities
The effects of osmotic stress on G1730 expression, and the phenotype seen in 35S::G1730 lines, indicated that the gene or its orthologs can be used to engineer plants with increased tolerance to abiotic stresses such as drought, salt, or cold.
G1753 (SEQ ID NO: 271)
Published Information
G1753 (At2g36450) was identified as part of the chromosome 2 clone F1O11 (GenBank accession AC006919).
Experimental Observations
The function of G1753 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1753 produced changes in Arabidopsis shoot architecture. 35S::G1753 transformants generally displayed reduced internode elongation in the inflorescence. Overall, this gave the plants a shorter, bushier appearance compared to wild type.
Two out of the three G1753 overexpressing lines showed a increase in the germination efficiency on media containing high concentrations of sucrose, indicating the gene is involved in sugar metabolism and/or signaling. In this context, it is striking that expression of the endogenous G1753 in wild-type plants was only detected in siliques, indicating that G1753 could be involved in sugar sensing processes in early seed development.
Utilities
G1753 could be used to create dwarf and compact forms of ornamental plants in horticulture markets. Dwarf and compact forms of ornamental plants are extremely popular among consumers. They represent a lucrative market for breeders and growers alike, but currently for many varieties, suitable dwarf breeding lines are either unavailable or difficult to integrate into existing germ-lines. Therefore, currently, many ornamental plants are sprayed with expensive chemical growth regulators to reduce height and increase compactness. Overexpression of a gene with G1753 activity could potentially alleviate this requirement.
The results of physiological assays indicate that G1753 could be used to alter the sugar signaling in plants.
If the physiological phenotype is related to osmotic stress, the gene could be used to engineer cold and dehydration tolerance.
G1779 (SEQ ID NO: 275)
Published Information
G11779 was identified from the Arabidopsis genomic sequence (GenBank accession number AL049483) based on its sequence similarity within the conserved domain to other GATA related proteins in Arabidopsis.
Experimental Observations
The function of this gene was initially studied by knockout analysis. Plants homozygous for a T-DNA insertion in G1779 were wild type for all assays performed.
Gene expression profiling using RT-PCR showed that G1779 is expressed in all tissues, albeit at higher levels in leaves.
The function of G1779 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1779 resulted in plants that showed enhanced tolerance to chilling stress when grown under low temperatures for an extended period of time. The majority of 35S::G1779 plants were wild type in morphological analyses that were performed.
Utilities
G1779 might be used to improve chilling tolerance.
G1792 (SEQ ID NO: 277)
Published Information
G1792 was identified in the sequence of BAC clone K14B15 (AB025608, gene K14B15.14). No information is available about the function(s) of G1792.
Closely Related Genes from Other Species
G1792 shows sequence similarity, outside of the conserved AP2 domain, with a protein from tomato, represented by EST sequence AI776626 (AI776626 EST257726 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLER19A14, mRNA sequence). No functional information is available about this tomato gene.
Experimental Observations
The function of G11792 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G1792 plants were more tolerant to the fungal pathogens Fusarium oxysporum and Botrytis cinerea : they showed fewer symptoms after inoculation with a low dose of each pathogen. These results were confirmed using individual T2 lines. FIG. 7C shows a G1792 overexpressing line (labeled G1792-12; on left) and wild-type plants (on right) five days after inoculation with Botrytis cinerea , showing the chlorosis and hyphal growth in the latter control plants but not in the former overexpressors. Additional, experiments have confirmed that 35S::G1792 plants also showed increased tolerance to challenge with Erysiphe . Five days after inoculation with Fusarium oxysporum , the G1792 overexpressors, as seen on the left in FIG. 7D , showed little or no chlorosis, as compared with wild-type plants on the right of FIG. 7D .
The effect of G1792 overexpression in increasing tolerance to pathogens received further, incidental confirmation. T2 plants of 35S::G1792 lines 5 and 12 were being grown (for other purposes) in a room that suffered a serious powdery mildew infection. For each line, a pot of 6 plants was present in a flat containing 9 other pots of lines from unrelated genes. In either of the two different flats, the only plants that were free from infection were those from the 35S::G1792 line. This observation indicated that G1792 overexpression might increase resistance to powdery mildew. Interestingly, G1792 was ubiquitously expressed, but appeared to be induced by salicylic acid.
35S::G1792 overexpressing plants showed more tolerance to growth under nitrogen-limiting conditions. In a root growth assay under conditions of limiting N, 35S::G1792 lines were less stunted. In a germination assay that monitors the effect of C on N signaling through anthocyanin production on high sucrose plus and minus glutamine (Hsieh et al. (1998) Proc. Natl. Acad. Sci . U.S.A) 95: 13965–13970), the 35S::G1792 lines made less anthocyanin, showed greater cotyledon expansion and had more root growth on high sucrose medium supplemented with glutamine ( FIG. 7A ) than control plants ( FIG. 7B ), indicating that the gene could be involved in the plants' ability to monitor their carbon and nitrogen status.
35S::G1792 overexpressing plants were larger and greener than wild-type control plants in a soil-based drought assay.
G1792 overexpressing plants showed several mild morphological alterations: leaves were dark green and shiny, and plants bolted, subsequently senesced, slightly later than wild-type controls. Among the T1 plants, additional morphological variation (not reproduced later in the T2 plants) was observed: many showed reductions in size as well as aberrations in leaf shape, phyllotaxy, and flower development.
Utilities
G1792 or its equivalogs could be used to engineer pathogen-resistant plants.
In addition, G1792 or its equivalogs could also be used to improve seedling germination and performance under conditions of limited nitrogen, and plants with enhanced drought tolerance.
G1796 (SEQ ID NO: 279)
Published Information
G1796 (At1g12980) is found in the sequence of BAC clone F3F19, GenBank accession number AC007357 (nid=4662618). G1796 was identified by Banno et al. ((2001) Plant Cell 13: 2609–2618) as ESR1 (Enhancer of Shoot Regeneration) in a screening for Arabidopsis cDNAs that can confer cytokinin-independent shoot formation from root cultures when overexpressed. The authors found enhanced shoot regeneration when a chemically inducible system was used for transient expression of ESR1. Transformation of Arabidopsis plants with a 35S::ESR1 construct using the flower vacuum infiltration method strongly inhibited normal leaf development. Only one transgenic 35S::ESR1 plant was obtained which produced dark green calli suggesting that ESR1 enhances shoot regeneration but interferes with the subsequent differentiation of plant cells.
G1796 was found to be included in patent application WO0200903.
Experimental Observations
The intronless G1796 gene was cloned from genomic DNA for overexpression. The function of the gene was analyzed using transgenic plants in which G1796 was expressed under the control of the 35S promoter. Overexpression of G1796 caused severe growth defects: seedlings were generally distinctly small and formed rather dark curled leaves. The growth arrest at very early seedling stages was also found by Banno et al. (2001) supra. The thickened club-like carpels, and the changes found in the structure of the inflorescences could be related to the function of G1796 in organogenesis, but were not specifically described by Banno et al. (2001) supra.
G01796 was expressed at low level in root, flower and rosette, but not in stems, siliques, embryos or germinating seeds.
Utilities
The use of G11796 for plant regeneration after transformation has been described by Banno et al. (2001) supra.
G1796 might be used to manipulate fruit size and shape.
Additionally, if the dark coloration of 35S::G1796 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G1797 (SEQ ID NO: 281)
Published Information
G1797 was identified within P1 clone MJM18 (chromosome 5, GenBank accession AB025623) as one of a pair of novel, highly related, tandemly arranged MADS box genes (the other gene was G1798). A functional characterization of G1797 remains to be published.
Experimental Observations
To assess the function of G1797, transgenic Arabidopsis lines were analyzed in which the gene was overexpressed from a CaMV promoter. 35S::G1797 transformants were very early flowering, had curled leaves, and retained outer whorl floral organs for a prolonged period following pollination and silique outgrowth. These phenotypes indicated that G1797 might influence genetic pathways that regulate flowering time or floral organ senescence and abscission. However, despite these changes in growth and development, 35S::G1797 lines displayed a wild type response in all of the physiological assays.
It should be noted that accelerated flowering and changes in flower morphology were also observed as a result of overexpression of the putative paralog, G1798, indicating that the two genes have related functions. Two other related genes, G627 and G1011, also produced very similar effects to G1797 and G1798 when overexpressed.
Interestingly, equivalent effects on perianth organs to those described above were obtained by Fernandez et al. ((2000) Plant Cell 12: 183–198) through overexpression of AGAMOUS-LIKE 15 (AGL15). G1797 and AGL15 occupy different clades within the MADS family, but the similarity in phenotype may indicate that they act in common pathways.
Utilities
The accelerated switch to reproductive growth seen in 35S::G1797 plants, indicated that the gene or its equivalogs could be used to manipulate flowering time in commercial species. Specifically, G1797 could be used to accelerate flowering, or eliminate any requirement for vernalization. Conversely, it is possible that the activity of G1797 or its equivalogs could be modified to delay flowering. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
The effects on flower development are also of commercial interest; the persistence of outer whorl organs following pollination in 35S::G1797 lines indicated that the gene or its equivalogs could be applied to ornamental plants to prolong the life of blooms.
G1798 (SEQ ID NO: 283)
Published Information
G1798 was identified within P1 clone MJM18 (chromosome 5, GenBank accession AB025623) as one of a pair of novel, highly related, tandemly arranged MADS box genes (the other gene was G1797). A functional characterization of G1798 remains to be published.
Experimental Observations
To assess the function of G1798, we analyzed transgenic Arabidopsis lines in which the gene was overexpressed from a CaMV promoter. 35S::G1798 transformants were very early flowering, had curled leaves, were very small and displayed severe abnormalities in flower development. As a result of such defects, the plants showed very poor fertility and insufficient seed was obtained to perform physiological assays. Additionally, a number of 35S::G1798 lines displayed terminal flowers, indicating that the gene could influence meristem determinacy.
It should be noted that accelerated flowering and changes in flower development were also observed as a result of overexpression of the putative paralog, G11797, indicating that the two genes have related functions. Interestingly, 35S::G1797 lines exhibited delayed floral organ abscission; such a phenotype might also have been prevalent in 35S::G1798 plants, but could have been masked by the severe sterility of these lines. Two other related genes, G627 and G1011 also produced very similar effects to G1797 and G1798 when overexpressed.
Utilities
The accelerated switch to reproductive growth seen in 35S::G1798 plants, indicated that the gene or its equivalogs could be used to manipulate flowering time in commercial species. Specifically, G1798 could be used to accelerate flowering, or eliminate any requirement for vernalization. Conversely, it is possible that the activity of G1798 or its equivalogs could be modified to delay flowering. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
The effects on flower and inflorescence development are also of commercial interest and indicated that the gene or its equivalogs might be used to manipulate floral traits such as sterility or fruit development, or to produce novel plant architectures.
G1816 (SEQ ID NO: 287)
Published Information
G11816 is a member of the MYB-related class of transcription factors. The gene corresponds to TRIPTYCHON (TRY), and has recently been shown to be involved in the lateral inhibition during epidermal cell specification in the leaf and root (Schellmann et al. (2002) EMBO J. 21: 5036–5046). The model proposes that TRY (G1816) and CPC (G225) function as repressors of trichome and atrichoblast cell fate. TRY loss-of-function mutants form ectopic trichomes on the leaf surface. TRY gain-of-function mutants are glabrous and form ectopic root hairs.
Experimental Observations
The complete sequence of G1816 was determined. The function of the gene was studied using transgenic plants in which G1816 was expressed under the control of the 35S promoter. Consistent with the morphological phenotypes published for the 35S::TRY overexpressors, the transgenic plants were glabrous and form ectopic root hairs. These transgenic lines were also more tolerant to growth under nitrogen-limiting conditions, both in a germination assay as well as a root growth assay on older seedlings. In addition to the nitrogen-limiting tolerance phenotypes observed in these transgenic lines, the 35S::G1816 plants were also insensitive to growth retardation effects of germination on conditions of high glucose, indicating that this gene could play a role in sugar sensing responses in the plant or osmotic stress tolerance. Genes for many sugar-sensing mutants are allelic to genes involved in abscisic acid and ethylene signaling (Rolland et al. (2002) Plant Cell 14: Suppl. S185–S205). Therefore, G1816 could also be involved in hormone signaling pathways.
Utilities
The phenotypic effects of G1816 overexpression, such as the increase in root hair formation and the increase in seedling vigor observed in a germination assay on high glucose media, indicated that the gene or its orthologs can be used to engineer plants with increased tolerance to abiotic stresses such as drought, salt, heat or cold.
In addition, the enhanced performance of G1816 overexpression lines under low nitrogen conditions indicated that the gene or its orthologs could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
The effect of G1816 overexpression on insensitivity to glucose in a germination assay, indicated that the gene or its orthologs could be involved in sugar sensing responses in the plant.
G1816 or its orthologs could also be used to alter anthocyanin production and trichome formation in leaves.
The potential utilities of genes involved in anthocyanin production include alterations in pigment production for horticultural purposes and increase stress resistance perhaps in combination with other transcription factors. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. In addition, several flavonoid compounds have health promoting effects such as the inhibition of tumor growth and cancer, prevention of bone loss and the prevention of the oxidation of lipids.
Given that the phenylpropanoid biosynthetic pathway (from which anthocyanins are produced) feeds into the pathways for the production of a number of other classes of secondary metabolites, such as lignins and tannins, changing the activity of G1816 or its orthologs might also influence the levels of those types of compounds.
G1837 (SEQ ID NO: 295)
Published Information
G1837 (At5g54480) was identified as part of the BAC clone F24B18, GenBank accession number AB026634 (nid=4757390).
Experimental Observations
The function of the gene was analyzed using transgenic plants in which a G1837 genomic clone was expressed under the control of the 35S promoter. Transgenic plants overexpressing G1837 showed increased tolerance to NaCl in the root inhibition assay, and also a potential enhancement of chilling tolerance. Under normal growth conditions, 35S::G1837 lines showed wild-type morphology.
Utilities
G1837 could be used to engineer increased salt stress tolerance.
G1840 (SEQ ID NO: 297)
Published Information
G1840 (At5g67010) was identified as part of TAC clone K8A10 (GenBank accession AB026640).
Experimental Observations
Overexpression of G1840 induced necrosis and death of patches of tissue in aerial part of the plant, indicating that it might be influence pathways of disease response, programmed cell death, or senescence. At early stages of development, 35S::G1840 seedlings appeared normal. However, towards the end of the rosette phase, these plants displayed rather broad flat dark leaves with short petioles. Randomly distributed, brown specks of necrotic tissue became visible on the leaves at around this time. Similar effects were noted in the inflorescence; in severely affected plants, the entire inflorescence tips became brown and withered away without producing seeds. This phenomenon was seen in both primary and secondary inflorescences, and plants with a strong phenotype developed a very short, bushy architecture.
A related AP2 family gene, G1749, was analyzed, and found to produce similar effects to G1840 when overexpressed.
Utilities
The overexpression phenotype indicates G1840 could have a role in regulating programmed cell death. Such a function could have various applications. The gene, its targets, or its equivalogs could be used to induce cell death in a controlled manner in specific tissues or in response to pathogen attack. For example, if the gene was specifically active in gametes or reproductive organs, the gene or its equivalogs might be used to achieve male or female sterility. Alternatively, in the latter scenario, the gene or its equivalogs might restrict the spread of a pathogen infection through a plant.
G1863 (SEQ ID NO: 303)
Published Information
G1863 was identified by amino acid sequence similarity to rice Growth-regulating-factor1 (GRF1), which has a potential role in the regulation of stem growth (Knaap et al. (2000) Plant Physiol. 122: 695–704). G1863, which has also been referred to as Arabidopsis GRL3, is found in the sequence of chromosome II section 199 of 255 (GenBank accession AC006919.5 GI:6598632), released by the Arabidopsis Genome Initiative. No information related to the functional characterization of G1863 is currently available from the public literature.
Experimental Observations
G1863 was found to be ubiquitously expressed, but had lower levels of expression in the stems of shoots than in other tissues. It was also determined that homozygotes for a T-DNA insertion within G1863 showed increased sensitivity to NaCl in germination assays.
35S::G1863 overexpressing transformants displayed a wild-type response in the physiology assays, but did display a number of morphological phenotypes. Plants that overexpress G1863 had larger leaves that had higher levels of chlorophyll per unit area. These plants were dark in coloration, showed changes in leaf shape, and delayed flowering.
Utilities
G1863 or its orthologs could be used to generate salt or drought tolerant crops.
The overexpression data indicate that the gene could have a number of additional applications.
The delayed flowering displayed by 35S::G1863 transformants indicated that the gene or its orthologs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Conversely, the activity of G1863 or its orthologs might be modified to accelerate flowering, or eliminate any requirement for vernalization.
This transcription factor or its orthologs could be used to improve plant productivity through increased biomass or yield and/or improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield. With regard to the former, consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of age-related macular degeneration (ARMD), the leading cause of blindness in elderly people.
The changes in leaf shape shown by 35S::G1863 plants also indicated that the gene or its orthologs could be used to engineer changes in plant form.
G1893 (SEQ ID NO: 305)
Published Information
G1893 (At1g03790) was identified in the sequence of P1 clone MOE17 (GenBank accession number AB025629) based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about the function(s) of G1893.
Experimental Observations
G1893 is expressed ubiquitously at moderately low levels and was weakly induced by cold. The function of G1893 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1893 resulted in seedlings with square cotyledons. True leaves were small with serrated margins. Many initial transformants did not survive past early stages of growth. Those that did survive were small and produced few seeds. These seedlings also contained more anthocyanin.
G1893 is a paralog of G3062 and G1976. Similar to what was seen with overexpression of G1893, overexpression of G3062 produced highly deleterious effects on Arabidopsis growth and development. It was nearly impossible to obtain 35S::G3062 transformants. Only one line survived to maturity, but was markedly smaller than wild-type controls at all stages of development, and yielded few seeds. Overexpression of G1976 also produced alterations in leaf morphology and flower development, as well as causing an extreme decrease in overall plant size and fertility. Severely affected 35S::G1976 lines were tiny, and died at early stages. More moderate phenotypes that were observed were very small plants with dark green leaves with serrated leaf margins. Inflorescences were short and lacked internode elongation. Floral organs were mostly very poorly developed with short pedicels. Fertility of 35S::G1976 transformants was very poor and many plants completely failed to set seed.
Utilities
G1893 or its equivalogs may have a utility in modifying fertility, cotyledon shape or plant architecture.
G1928 (SEQ ID NO: 311)
Published Information
G1928 was identified in the sequence of P1 clone MCP4, GenBank accession number AB028610, released by the Arabidopsis Genome Initiative. There is no other published or public information about the function(s) of G1928.
Experimental Observations
The function of G1928 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Transgenic Arabidopsis plants overexpressing G1928 grew much more vigorously than wild-type plants at 4° C.; the plants were larger, less chlorotic and showed fewer symptoms of stress.
Utilities
Based on the effects of G1928 overexpression, the gene or its orthologs might be used to protect crops against low temperature conditions.
G1968 (SEQ ID NO: 321)
Published Information
G1968 (At1g26610) was identified in the sequence of BAC T1K7 (GenBank accession number AC013427), based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about the function of G1968.
Experimental Observations
The function of G11968 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1968 resulted in plants that were small and slow developing. Flowers were also small and had defects in organ formation. G1968 overexpressing lines contained more anthocyanins when grown under low nitrogen, or low nitrogen plus glutamine, in a germination assay. When grown on control plates, two lines exhibited size segregation. One line was wild type.
Utilities
On the basis of the response of G1968 to low nitrogen media, the gene might be useful in developing plants that are more tolerant to poor nutrient growth conditions.
G1983 (SEQ ID NO: 323)
Published Information
G1983 (At1g30790) was identified in the sequence of BAC F21M11 (GenBank accession number AC003027) based on its sequence similarity within the conserved domain to other C3H related proteins in Arabidopsis . There is no published or public information about the function of G1983.
Experimental Observations
The function of G1983 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1983 resulted in plants that were small, darker green and flowered late. 35S::G1983 plants were wild type in all physiological analyses that were performed.
Utilities
Based on the delayed flowering of G1983 overexpression lines, the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
If the dark coloration of 35S::G1983 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G1985 (SEQ ID NO: 325)
Published Information
G1985 was identified in the sequence of BAC F3L24, GenBank accession number AC011436, released by the Arabidopsis Genome Initiative. There is no other published or public information about G1985.
Experimental Observations
The 5′ and 3′ ends of G1985 were determined by RACE PCR, and the gene function was analyzed via overexpression lines.
Overexpression of G1985 produced a spectrum of alterations in Arabidopsis development. However, the most dramatic effect was observed in the inflorescence, where G1985 appeared to inhibit reproductive development and caused a reversion towards vegetative growth. 35S::G1985 plants were very small, slow developing, formed rather dark curled leaves. The inflorescences from these plants were generally stunted, and carried flowers that often had underdeveloped organs and poor pollen production. Strikingly, however, inflorescences typically showed an increase in vegetative characteristics and often formed small aerial rosettes. Additionally, in some cases, the inflorescence meristem apparently reverted back to initiating leaf primordia once it had entered the phase of flower initiation.
Floral reversion is extremely rare in Arabidopsis , especially under inductive light conditions such as those in which these experiments were performed. In wild-type plants, the shoot meristem, on becoming an inflorescence meristem, usually forms 2–3 single cauline leaf primordia (which develop secondary shoots in their axils) and then initiates floral meristems until senescence occurs. The inflorescence meristem displays a strong commitment to flower formation, and usually never switches back into a phase of leaf production once flower initiation has commenced. However, in some species (e.g. Impatiens ) floral reversion does occur, and is an important means by which the plant achieves developmental plasticity in response to changing environmental conditions (Battey et al. (2002). Curr. Opin. Plant Biol. 5: 62–68; Battey (2000) J. Exp. Bot. 51: 1769–1780).
Utilities
The experimental results obtained with G1985 overexpressors indicate that the gene or its orthologs can modulate the developmental programs, which regulate phase change and developmental plasticity of the shoot meristem. In particular, the gene might be used to manipulate seasonality and influence whether plants display an annual or perennial habit.
G1988 (SEQ ID NO: 327)
Published Information
G1988 (At3g21150) is in P1 clone MSA6 (GenBank accession number AP000604) and was identified based on its sequence similarity within the conserved domain to other CONSTANS-like related proteins in Arabidopsis . There is no published or public information about the function of G1988.
Experimental Observations.
The function of G1988 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Evidence from physiological and morphological assays indicates that G1988 may play a role in developmental processes regulated by light; 35S::G1988 seedlings displayed longer hypocotyls, elongated petioles, and a number of lines flowered early.
When grown on limited phosphate, all lines appeared larger and had more root growth than controls. Seedlings germinated on plates that contained limited nitrogen (supplemented with glutamine) appeared less stressed than controls.
Utilities
Based on the results from physiological assays, G1988 might be used to engineer plants that show enhanced growth and survivability in low nutrient environments.
G1988 could also have a role in modulating developmental processes regulated by light, such as shade avoidance. Eliminating shading responses could lead to increased planting densities with subsequent yield enhancement. The gene might also be useful in manipulating flowering time.
G1995 (SEQ ID NO: 333)
Published Information
G1995 (At3g58070) is in BAC T10K17 (GenBank accession number AL132977) and was identified based on its sequence similarity within the conserved domain to other zinc finger DOF-related proteins in Arabidopsis . There is no published or public information about the function of G1995.
Experimental Observations
The function of G1995 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G1995 resulted in plants that had flowers with increased trichome density on sepals and ectopic trichomes on carpels. The flowers also had rather poor pollen production and many of the lines yielded only relatively small quantities of seed. One line displayed aerial rosette like structures and had floral organs that were converted towards a bract-like identity. In physiological analyses, G1995 overexpressors showed size segregation and a slight increase in sensitivity to nutrient limitation.
Utilities
Based on the results from physiological assays, G1995 might be used to engineer plants that show enhanced growth and survivability in low nutrient environments.
The morphological effects of G1995 overexpression indicate a number of potential applications relating to increasing or changing trichome density:
Trichome glands on the surface of many higher plants produce and secrete exudates that give protection from the elements and pests such as insects, microbes and herbivores. These exudates may physically immobilize insects and spores, may be insecticidal or anti-microbial or they may produce allergens or irritants to protect against herbivores. Trichomes have also been suggested to decrease transpiration by decreasing leaf surface airflow, and by exuding chemicals that protect the leaf from the sun.
Depending on the plant species, varying amounts of diverse secondary biochemicals (often lipophilic terpenes) are produced and exuded or volatilized by trichomes. These exotic secondary biochemicals, which are relatively easy to extract because they are on the surface of the leaf, have been widely used in such products as flavors and aromas, drugs, pesticides and cosmetics. One class of secondary metabolites, the diterpenes, can effect several biological systems such as tumor progression, prostaglandin synthesis and tissue inflammation. In addition, diterpenes can act as insect pheromones, termite allomones, and can exhibit neurotoxic, cytotoxic and antimitotic activities. As a result of this functional diversity, diterpenes have been the target of research several pharmaceutical ventures. In most cases where the metabolic pathways are impossible to engineer, increasing trichome density or size on leaves may be the only way to increase plant productivity.
Thus, the use of G1995 and its homologs to increase trichome density, size or type may therefore have profound utilities in so called molecular farming practices and increasing the yield of cotton fibers.
If the effects on trichome patterning and/or aerial rosette formation reflect a general change in heterochronic processes, G1995 or other clade members, might be used to modify the way meristems and/or cells develop during different phases of the plant life cycle. In particular, altering the timing of phase changes could afford positive effects on yield and biomass production.
G2041 (SEQ ID NO: 341 and SEQ ID NO: 2110)
Published Information
The transcriptional regulator G2041 was identified by amino acid sequence similarity to proteins of the SWI/SNF family of chromatin remodeling factors. G2041 is found in the sequence of the chromosome 3, BAC clone T12K4 (AL138640.1 GI:6899910), released by the Arabidopsis Genome Initiative. No additional public information related to the functional characterization of G2041 is available.
Experimental Observations
The function of G2041 was analyzed through its overexpression in Arabidopsis; 35S::G2041 lines displayed no consistent morphological changes when compared to control plants. However, the overexpression lines were more tolerant to salt stress in a germination assay. It should be noted that since a truncated version of the gene (SEQ ID NO: 2110) was overexpressed, the phenotype obtained could be a dominant negative type effect.
Utilities
The results of physiological assays indicate that G2041 or its equivalogs could be modify abiotic stress responses. Given the salt resistance exhibited by 35S::G2041 transformants, the gene or its equivalogs might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions.
G2051 (SEQ ID NO: 343)
Published Information
G2051 corresponds to AT1G32510, annotated by the Arabidopsis Genome initiative. No information is available about the function(s) of G2051.
Experimental Observations
The function of G2051 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. The phenotypes of the 35S::G2051 transgenic Arabidopsis plants were wild-type in morphology.
G2051 overexpressing lines were more tolerant of chilling stress in a germination assay. Two of the three lines analyzed showed the response.
Utilities
Based on the phenotype observed in 35S::G2051 transgenic plants, the gene or its equivalogs could be engineered to manipulate the response to abiotic stresses, such as cold during germination. For example, a gene that enhanced germination and seedling vigor in the cold would have tremendous utility in allowing seeds to be planted earlier in the season with a higher survival rate.
G2060 (SEQ ID NO: 345)
Published Information
G2060 corresponds to gene AT1g69810, and it has also been described as WRKY36. No information is available about the function(s) of G2060.
Experimental Observations
G2060 is ubiquitously expressed in Arabidopsis , with slightly higher levels of transcript being found in roots than in other samples.
A T-DNA insertion mutant for G2060 was analyzed and shown to display a wild-type morphology, and was also wild-type in its response to the physiological analyses that were performed.
Transgenic plants in which G2060 was expressed under the control of the 35S promoter were then generated. No consistent morphological or developmental alterations were observed as a consequence of G2060 overexpression. However, 35S::G2060 seedlings were more tolerant to salt stress in a root inhibition assay when compared to the wild-type controls.
Utilities
Based on the increased salt tolerance exhibited by the 35S::G2060 lines in physiology assays, this gene might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
G2085 (SEQ ID NO: 355)
Published Information
G2085 was identified in the sequence of BAC T22A6, GenBank accession number AL078637, released by the Arabidopsis Genome Initiative.
G2085 appears to correspond to ZIM (at residue 306 near the carboxyl terminus, leucine is replaced by methionine in ZIM, compared to isolated cDNA, and the genomic sequence released by AGI). Nishii et al. ((2000) Biosci. Biotechnol. Biochem. 64: 1402–1409) isolated ZIM by differential screening of an arrayed normalized cDNA library from inflorescence tissue of Arabidopsis (Takemura et al. (1999) DNA Res. 6: 275–282). In addition to the GATA domain, ZIM contains a basic region with a sequence resembling a nuclear localization signal, and an acidic region. The nuclear localization of ZIM was detected using GFP as a reporter. Based on its expression pattern, zinc finger domain, and nuclear localization, the authors suggest that ZIM is involved in inflorescence and flower development.
Experimental Observations
G2085 was determined to be constitutively expressed throughout the plant, and that its expression is markedly repressed by a variety of stress conditions such as abscisic acid, cold, osmotic stress, and Erysiphe . G2085 was analyzed via a homozygous T-DNA insertion mutant and that line appeared wild-type in all assays.
The complete sequence of G2085 was determined, and G2085 overexpression lines were generated. Many of the plants overexpressing G2085 had small, dark colored, hirsute, inner rosette leaves. Altered seed morphology and increased seed size was also noted. Trichome density was increased.
In each set of primary transformants, many of the lines were smaller than wild type controls. In particular, the adult rosette leaves of around half of the plants from the second set were noted to be small, dark in coloration, and have a rather high trichome density. Additionally, alterations in morphology were observed in the seeds from a small number of T1 lines from each set. From the first set, seeds from 2/18 lines were larger than controls, and in the second set, 4/16 lines had rather pale seeds, and two of these showed seeds that were also large.
Utilities
The promoter of G2085 may also have utility as a promoter that can be down-regulated in response to a variety of stresses.
Based on the overexpression phenotypes, G2085 or its orthologs might be used to manipulate plant growth and development. Based on the increase in seed size of the 35S::G2085 transgenic lines, G2085 could be increasing the size of the embryo and that could enhance seed traits such as seed oil or seed protein content or yield. Additionally, G2085 might be used to modify trichome density. Additionally, if the dark coloration of 35S::G2085 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
The morphological effects of G2085 overexpression indicate a number of potential applications relating to increasing or changing trichome density: Thus, the use of G2085 and its equivalogs to increase trichome density, size or type may therefore have profound utilities in so called molecular farming practices and increase the yield of cotton fibers.
G2133 (SEQ ID NO: 1495)
Published Information
G2133 corresponds to gene F26A9.11 (AAF23336). No information is available about the function(s) of G2133.
Closely Related Genes from Other Species
G2133 does not show extensive sequence similarity with known genes from other plant species outside of the conserved AP2/EREBP domain.
Experimental Observations
The function of G2133 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.
G2133 expression was detected in a variety of tissues: flower, leaf, embryo, and silique samples. Its expression might be altered by several conditions, including auxin treatment, osmotic stress, and Fusarium infection. Overexpression of G2133 caused a variety of alterations in plant growth and development: delayed flowering, altered inflorescence architecture, and a decrease in overall size and fertility.
At early stages, 35S::G2133 transformants were markedly smaller than controls and displayed curled, dark-green leaves. Most of these plants remained in a vegetative phase of development substantially longer than controls, and produced an increased number of leaves before bolting. In the most severely affected plants, bolting occurred more than a month later than in wild type (24-hour light). In addition, the plants displayed a reduction in apical dominance and formed large numbers of shoots simultaneously, from the axils of rosette leaves. These inflorescence stems had short internodes, and carried increased numbers of cauline leaf nodes, giving them a very leafy appearance. The fertility of 35S::G2133 plants was generally very low. In addition, G2133 overexpressing lines were found to be more resistant to the herbicide glyphosate in initial and repeat experiments.
No alterations were detected in 35S::G2133 plants in the biochemical analyses that were performed.
G2133 is a paralog of G47, the latter having been known from earlier studies to confer a drought tolerance phenotype when overexpressed. It was thus not surprising when G2133 was also shown to induce drought tolerance in a number of 35S::G2133 lines challenged in soil-based drought assays. Results with two of these lines are shown in FIGS. 10A and 10B , which compare the recovery of these lines from eight days of drought treatment with that of wild-type controls. After re-watering, all of the plants of both G2133 overexpressor lines became reinvigorated, and all of the control plants died or were severely affected by the drought treatment.
Utilities
G2133 could be used for the generation of glyphosate resistant plants, and to increase plant resistance to oxidative stress.
G2133 can be used to increase the tolerance of plants to drought and likely to other osmotic stresses as well.
G2142 (SEQ ID NO: 365)
Published Information
G2142 was identified by amino acid sequence similarity to other HLH/MYC proteins. G2142 is found in the sequence of the chromosome 1 BAC clone T6L1 (GenBank accession number AC011665, nid=g6358759), released by the Arabidopsis Genome Initiative. No information related to the functional characterization of G2142 is currently available from the public literature.
Experimental Observations
The function of G2142 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. A small number of the 35S::G2142 plants displayed a slight acceleration of flowering compared to controls. Additionally, G2142 overexpressors were more tolerant to phosphate deprivation in a root growth assay, but this effect was rather subtle.
Utilities
The results of physiological assays indicate that G2142 could be used to improve plant performance in conditions of limited phosphate.
G2146 (SEQ ID NO: 367)
Published Information
The sequence of G2146 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AC012393, nid=6143859, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
The function of G2146 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2146 resulted in plants that displayed a mild increase in the time to flowering, darker coloration, and inflorescences that were shorter and bushier than those of wild-type plants.
Utilities
G2146 could be used to generate plants that flower late or have altered leaf coloration and plant architecture. The delayed flowering displayed by 35S::G2146 transformants indicates that the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Additionally, the dark coloration of 35S::G2146 lines may reflect an increase in biochemical composition; the gene may thus be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G2207 (SEQ ID NO: 371)
Published Information
G2207 (At1g20640) was identified as part of the BAC clone F5M15, GenBank accession number AC027665 (nid=8096769).
Experimental Observations
The complete sequence of G2207 was determined. The function of the gene was analyzed using transgenic plants in which a genomic clone for G2207 was expressed under the control of the 35S promoter. In germination assays, 35S::G2207 lines showed increased tolerance to osmotic stress under conditions of high salt or high sucrose and were less sensitive to abscisic acid. All these phenotypes indicate that G2207 is involved in the plant response to dehydration stress. A small number of the lines also showed delayed flowering, indicating that the gene regulates the timing of the floral transition.
The bZIP-NIN gene G2207 does not share significant homology to any of the bZIP genes, for some of which a role in abscisic acid signaling has been reported (ABF1=G2071, ABF2=G3028, ABF3=G570, ABF4=G1058; Choi et al. (2000) J. Biol. Chem. 275: 1723–1730).
Utilities
G2207 appears to affect ABA sensitivity. ABA is one of the key signal molecules in the stress response pathways. G2207 may have a utility in modifying ABA responses such as seed dormancy, seed development, and cold and/or drought tolerances.
In particular, based on the increased tolerance to high levels of salt or sucrose, exhibited by the 35S::G2207 lines in physiology assays, this gene might be used to engineer crops and trees that can flourish in salinified soils, or under drought conditions.
Although the increased sucrose tolerance observed for 35S::G2207 lines is most likely related to a general dehydration stress tolerance, the gene might be involved in sugar sensing. Thus G2207 might also be used to generate crop plants with altered sink source relations.
The late flowering shown by 35S::G2207 lines indicates that the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Additionally, if the dark coloration of 35S::G2207 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G2239 (SEQ ID NO: 379)
Published Information
G2239 was identified in the sequence of BAC T12H1, GenBank accession number AC009177, released by the Arabidopsis Genome Initiative.
G2239 corresponds to ATL6, a member of the ATL (RHE) gene family (Jensen et al. (1998) FEBS Lett. 436: 283–287; Martinez-Garcia et al. (1996) Mol. Gen. Genet. 252: 587–596; Salinas-Mondragon et al. (1999) Plant Mol. Biol. 40: 579–590). Members of the ATL gene family contain, in addition to the RING C3H2C3 domain, an n-terminal transmembrane domain. Fungal elicitors and cycloheximide applications caused rapid induction of ATL6 and ATL2 (G649) expression. However, induced ATL6 expression was found to be more stable than that of ATL2. This difference might be accounted for by the fact that the ATL6 3′ UTR lacks a DST element (an mRNA stability determinant found in SAUR transcripts), whereas this element is found in the 3′ UTR of ATL2. The authors suggest that ATL6 and ATL2 may function in plant-pathogen interactions.
Experimental Observations
The complete sequence of G2239 was determined and its function was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.
G2239 overexpression lines were greener and contained less anthocyanin when grown on low nitrogen media supplemented with sucrose or sucrose plus glutamine. However, the phenotype of 35S::G2239 plants was wild-type in all morphological assays performed.
Utilities
The enhanced performance of G2239 overexpression lines under low nitrogen conditions indicated that the gene or its orthologs could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
The observation that 35S::G2239 lines made less anthocyanin on high sucrose plus glutamine, indicated that G2239 might be used to modify carbon and nitrogen status, and hence assimilate partitioning.
G2317 (SEQ ID NO: 389)
Published Information
G2317 is a novel member of the Myb-related family of transcription factors. G2317 corresponds to gene At1g18330, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G2317.
Experimental Observations
The complete sequence of G2317 was determined. The function of G2317 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2317 plants did not show consistent alterations in morphology or development.
When analyzed in physiological assays, each of three 35S::G2317 lines showed more tolerance to salt stress by displaying more root growth in a root growth assay. G2317 overexpressing lines also displayed larger size and less chlorosis in high salt (150 mM) at the seedling stage when compared to wild-type plants.
One transgenic line showed enhanced performance when germinated under cold conditions.
Utilities
The results of physiological assays indicate that G2317 could be modify abiotic stress responses.
Given the salt resistance exhibited by 35S::G2317 transformants, the gene or its orthologs might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions.
The enhanced performance of a line of 35S::G2317 seedlings under chilling conditions indicated that the gene or its orthologs might be applied to engineer crops that show better growth under cold conditions.
G2319 (SEQ ID NO: 391 and SEQ ID NO: 2112)
Published Information
G2319 corresponds to AT3G09600, annotated by the Arabidopsis Genome initiative. No information is available about the function(s) of G2319.
Experimental Observations
The function of G2319 was analyzed using transgenic plants in which the gene was expressed under the control of the 35S promoter. Two different G2319 constructs were transformed into Arabidopsis.
P13388 contained a truncated version of the gene (SEQ ID NO: 2112) whereas P13446 contained a full-length clone. Transformants harboring P13446 exhibited wild-type morphology and exhibited wild-type phenotypes in response to the physiology assays. A marked delay in the onset of flowering was observed in ten of the eighteen lines transformed with the P13388 construct. Three lines co-transformed with P13388 showed more tolerance to salt stress in a root growth inhibition assay. When the assay was repeated, all thereof these lines repeated the phenotype.
Given that all of the phenotypic effects resulted from overexpression of a truncated version (SEQ ID NO: 2112) of the G2319 product, it might represent a dominant negative phenotype.
Utilities
Based on the phenotypes observed in morphological and physiological assays, G2319 or its equivalogs might be have a number of utilities.
Given the salt resistance exhibited by 35S::G2319 transformants, the gene or its equivalogs might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions.
The late flowering displayed by 35S::G2319 transformants indicated that the gene or its equivalogs might be used to delay the flowering of commercial species.
G2334 (SEQ ID NO: 393)
Published Information
G2334 was identified by amino acid sequence similarity to the rice Growth-regulating-factor1 (GRF1), which has a potential role in the regulation of stem growth in rice (Knapp et al (2000) Plant Physiol. 122: 695–704). It is found in the sequence of chromosome 3, BAC clone F8J2 (AL132969.2 GI:7629988), released by the Arabidopsis Genome Initiative. No information related to the functional characterization of G2334 is currently available from the public literature.
Experimental Observations
The function of G2334 was analyzed through its overexpression in Arabidopsis; 35S::G2334 lines displayed marked delay in the onset of flowering, developed large wrinkled dark green leaves, and had substantially greater vegetative biomass than wild-type controls.
It should be noted that the effects of G2334 overexpression are very similar to those produced by overexpression of a related gene G1863, indicating that the two genes might have overlapping functions.
Utilities
The overexpression data indicate that G2334 could have a number of applications.
The phenotypes displayed by 35S::G2334 transformants indicated that the gene or its equivalogs might be used to increase size or manipulate the flowering time of commercial species. Conversely, the activity of G2334 or its equivalogs might be modified to accelerate flowering, or eliminate any requirement for vernalization.
Additionally, if the altered coloration of 35S::G2334 plants reflects a change in biochemical composition, the gene or its equivalogs might be used to improve the nutraceutical value of foodstuffs, for example, by reducing the risk of ARMD, or increase photosynthetic capacity to improve yield.
The changes in leaf shape shown by 35S::G2334 plants indicated that the gene or its equivalogs could be used to engineer changes in plant form.
G2382 (SEQ ID NO: 401)
Published Information
The sequence of G2382 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AB020746, nid=3985949, based on its sequence similarity within the conserved domain to other triple-helix related proteins in Arabidopsis.
Experimental Observations
The function of G2382 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2382 resulted in plants that were insensitive to ABA treatment in a germination assay.
Utilities
G2382 appears to affect ABA sensitivity. ABA is one of key signal molecules in the stress response pathways. Therefore, G2382 may have a utility in modifying ABA responses such as seed development, seed dormancy, and cold and dehydration tolerance.
G2432 (SEQ ID NO: 407)
Published Information
G2432 (At1g29160) is in the sequence of BAC F28N24 (GenBank accession number AC021043) based on its sequence similarity within the conserved domain to other DOF related proteins in Arabidopsis . There is no published or public information about the function of G2432.
Experimental Observations
The function of G2432 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2432 resulted in very small plants with narrow cotyledons and poorly developed roots. As 35S::G2432 plants matured, tiny, round, vertically oriented leaves with extremely long petioles were observed, and flowering was delayed. Such features often indicate alterations in light regulated development. Many lines senesced without setting seed. The lines that did flower produced inflorescences with infertile flowers. Consequently, no seed was obtained for physiological analysis.
Utilities
The phenotype of plants overexpressing G2432 or its paralog G736 indicate that the former gene or its equivalogs might be used to manipulate developmental processes regulated by light, such as shade avoidance. Eliminating shading responses could lead to increased planting densities with subsequent yield enhancement. The gene might also be useful in modifying flowering time.
G2453 (SEQ ID NO: 411, SEQ ID NO: 2113 and SEQ ID NO: 2114)
Published Information
The YABBY transcription factor G2453 is referenced in the public literature as INNER NO OUTER (INO), and has an established role in the abaxial-adaxial patterning of Arabidopsis ovules (Baker et al. (1997) Genetics 145: 1109–1124; Villanueva et al. (1999) Genes Dev. 13: 3160–3169). In common with other transcription factors from the YABBY gene family, INO has an important function in specifying abaxial cell fate in organs (Baker et al. (1997) supra; Villanueva et al. (1999) supra; Siegfried et al. (1999) Development 126: 4117–4128). G2453 is found in the sequence of the chromosome 1 BAC F28C11 (AC007945.3 GI:6096767), released by the Arabidopsis Genome Initiative.
Experimental Observations
The function of G2453 was analyzed through its ectopic overexpression in Arabidopsis . The sequences that were used to confer the phenotypes disclosed here were SEQ ID NOs: 2113 and SEQ ID NO: 2114, which are both variants of the G2453 sequence. These experiments revealed a number of morphological changes that are consistent with the previously defined role of YABBYs in abaxial cell fate determination in leaves, floral organs and ovules. The plants were generally small, slow developing, and produced rather dark, curled leaves that had a wrinkled surface texture compared to those of wild type. Additionally, an effect was observed which has not been discussed in the public literature: G2453 overexpression resulted in accumulation of high levels of anthocyanins, particularly at early stages of seedling development.
A number of lines in which an antisense version of G2453 was overexpressed were also examined. These lines exhibited wild-type morphology but displayed enhanced tolerance to salt stress in a germination assay.
Utilities
Based on the published data, the gene or its equivalogs could potentially be used to modify leaf and flower development. In particular, the gene might be used to manipulate fruit traits.
The increased pigment levels and dark coloration observed in 35S::G2453 transformants point towards a number of utilities reducing the risk of ARMD by consumption of plants so altered.
Given that the 35S::G2453 plants likely had increased chlorophyll levels, the gene or its equivalogs might also be used to enhance photosynthetic capacity and yield.
G2453 or its equivalogs could also be applied to alter pigment production for horticultural purposes and to increase stress resistance. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. Since the phenylpropanoid biosynthetic pathway (from which anthocyanins are produced) feeds into the pathways for the production of a number of other classes of secondary metabolites, such as lignins and tannins, changing the activity of G2453 or its equivalogs might also influence the levels of those types of compounds.
Given the increased salt tolerance exhibited by the 35S::G2453 antisense lines, the gene or its equivalogs might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
G2457 (SEQ ID NO: 417)
Published Information
G2457 is known in the public literature as CRABS CLAW (CRC), which in common with other members of the YABBY family, plays an important role in specifying abaxial cell fate in leaves and floral organs (Alvarez et al (1999) Development: 126: 2377–2386; Bowman et al. (1999) Development: 126: 2387–2396; Eshed et al. (1999) Cell 99: 199–209; Siegfried (1999) Development 126: 4117–4128). G2457 is found in the sequence of the chromosome 1, BAC F23O10 (GenBank accession number AC018364.5; GI:12325073), released by the Arabidopsis Genome Initiative.
Experimental Observations
The function of G2457 was analyzed through its ectopic overexpression in Arabidopsis; 35S::G2457 lines exhibited a number of morphological changes, which are consistent with the previously defined role of CRC in abaxial cell fate determination in leaves, floral organs, and ovules. These plants showed distinctly narrow and curled leaves and a variety of floral defects.
The overexpression lines revealed an additional potential role for G2457 in the response to abiotic stress which has not previously been recognized in the published literature; all three of the 35S::G2457 lines tested performed better than wild-type controls on plates containing sodium chloride.
Utilities
Based on the effects of G2457 overexpression and the published data, the gene or its orthologs could potentially be used to modify leaf and flower, and fruit development.
Given the increased salt tolerance exhibited by the 35S::G2457 lines, the gene may be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
G2459 (SEQ ID NO: 419)
Published Information
G2459 was identified by amino acid sequence similarity to the Arabidopsis YABBY1. Transcription factors from the YABBY family play an important role in specifying abaxial cell fate in leaves and floral organs (Siegfried et al. (1999) Development 126: 4117–4128). G2459 is found in the sequence of the chromosome 2 clone T9J22 map B68 (AC002505.3 GI:20196938), released by the Arabidopsis Genome Initiative.
Experimental Observations
The function of G2459 was analyzed through its ectopic overexpression in Arabidopsis . The overexpression of G2459 revealed a number of morphological changes, which were consistent with the previously defined role of YABBYs in abaxial cell fate determination in leaves, floral organs and ovules. All the T1 plants were generally small, slow developing, and produced rather dark, curled leaves compared to those of wild type.
Additionally, an effect was observed which has not been discussed in the public literature: G2459 overexpression resulted in accumulation of high levels of anthocyanins, particularly at early stages of seedling development. This effect was exacerbated by stress conditions such as high levels of glucose.
Utilities
Based on the published data, the gene could potentially be used to modify leaf and flower development. In particular, the gene or its equivalogs might be used to manipulate fruit traits.
The increased pigment levels and dark coloration observed in 35S::G2453 transformants point towards a number of utilities reducing the risk of ARMD by consumption of plants so altered. Given that the 35S::G2453 plants likely had increased chlorophyll levels, the gene might also be used to enhance photosynthetic capacity and yield. G2453 or its equivalogs could also be applied to alter pigment production for horticultural purposes and to increase stress resistance. Flavonoids have antimicrobial activity and could be used to engineer pathogen resistance. In addition, several flavonoid compounds have health promoting effects such as the inhibition of tumor growth, prevention of bone loss and the prevention of the oxidation of lipids. Since the phenylpropanoid biosynthetic pathway (from which anthocyanins are produced) feeds into the pathways for the production of a number of other classes of secondary metabolites, such as lignins and tannins, changing the activity of G2453 or its equivalogs might also influence the levels of those types of compounds.
G2505 (SEQ ID NO: 425)
Published Information
G2505 is a novel member of the NAC family of transcription factors. G2505 corresponds to gene At4g10350, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G2505.
Experimental Observations
G2505 was expressed at low or non-detectable levels in most tissue types. Higher levels of transcript were found in roots compared to other tissues. No induction of G2505 expression in leaf tissue was detected in response to environmental stress-related conditions.
The effects of G2505 overexpression (overexpression construct P1533) were analyzed. Despite numerous repeated attempts, 35S::G2505 transformants could not be obtained and it was concluded that overexpression of the gene caused lethality during embryo or early seedling development.
Upon repeating the transformation with a new overexpression construct (P2776), the transformation frequency was very low and the few lines that were obtained were distinctly small and dark in coloration Only two of these lines produced sufficient seed for physiology assays to be performed. However, both of those lines displayed enhanced performance in a severe drought assay.
Utilities
The reduced sensitivity of 35S::G2505 lines in the dehydration stress assay indicated that the gene or its orthologs might be used to engineer crops with increased tolerance to drought, salt, freezing and chilling stress, or increased water use efficiency.
G2536 (SEQ ID NO: 431)
Published Information
G2536 is a novel member of the NAC family of transcription factors. G2536 corresponds to gene At3g44350, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G2536.
Experimental Observations
The complete sequence of G2536 was determined. The function of the gene was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2536 plants did not show consistent alterations in their response to the physiological analyses that were performed.
Overexpression of G2536 produced a striking effect on leaf development and caused both an increase in leaf size as well as an apparent delay in the onset of leaf senescence. At early stages, 35S::G2536 transformants appeared normal, except for a small number of lines, which displayed rather long petioles. However, towards the end of the rosette stage and the start of the inflorescence stage, a marked alteration in rosette morphology was noticeable; leaves were generally larger and broader than in wild type and often exhibited mild serrations on the margins. This effect became increasingly apparent as the plants aged. At the end of the lifecycle, the leaves of 35S::G2536 plants also remained green and showed a delay in senescence compared to wild type. Some of the 35S::G2536 lines showed a mild delay in the onset of flowering (up to about seven days in continuous light).
Utilities
The effects on leaf size and shape that were observed as a result of G2536 overexpression might also have commercial applications. Increased leaf size, or an extended period of leaf growth as a result of G2536 or ortholog expression modification, could increase photosynthetic capacity, and biomass, and have a positive effect on yield. The observed stay green phenotype or delay in the onset of senescence could also result in yield increases by increasing the period of photosynthetic productivity in source tissues.
G2550 (SEQ ID NO: 435)
Published Information
G2550 was initially identified within sequence released by the Arabidopsis genome initiative (gene F1B16.6 within BAC clone F1B16, chromosome I, GenBank accession, AC023754), as a gene encoding a novel homeodomain protein of the BEL1 class. No public data are available pertaining to the function of this gene.
Experimental Observations
The boundaries of G2550 were determined by RACE experiments, and transgenic lines were generated in which the gene was overexpressed from a 35S promoter. These transformants exhibited a wild-type response to physiological assays, but displayed a number of morphological phenotypes. 35S::G2550 lines were dark in coloration, displayed alterations in leaf shape, and formed shorter more compact inflorescences than controls.
Utilities
The changes in morphology shown by the 35S::G2550 transformants indicated that the gene or its orthologs could be used to manipulate inflorescence architecture and branching patterns in commercial species, to create varieties with more compact forms. In particular, dwarf and compact forms of ornamental plants are extremely popular among consumers. They represent a lucrative market for breeders and growers alike, but currently for many varieties, suitable dwarf breeding lines are either unavailable or difficult to integrate into existing germ-lines. Therefore, currently, many ornamental plants are sprayed with expensive chemical growth regulators to reduce height and increase compactness. Overexpression of a gene with G2550 activity could potentially alleviate this requirement.
The altered coloration of 35S::G2550 plants reflects a change in biochemical composition, and thus the gene or its orthologs might be used to improve the nutraceutical value of foodstuffs. For example, consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of age-related macular degeneration (ARMD), the leading cause of blindness in elderly people. Given that the 35S::G2550 plants likely had increased chlorophyll levels, the gene might be used to enhance photosynthetic capacity and yield.
G2567 (SEQ ID NO: 441)
Published Information
G2567 corresponds to ARF16 (Hagen and Guilfoyle (2002) Plant Mol. Biol. 49: 373–385; Liscum and Reed (2002) Plant Mol. Biol. 49: 387–400). G2567 was identified in genomic sequence (BAC accession number AL161576).
Experimental Observations
The complete cDNA sequence of G2567 was determined. The function of this gene was analyzed using transgenic plants in which G2567 was expressed under the control of the 35S promoter. Plants overexpressing G2567 showed enhanced tolerance to chilling stress in a growth assay. These plants were wild-type in morphology.
Utilities
G2567 may be used to engineer crop plants that are more tolerant to chilling stress.
G2571 (SEQ ID NO: 445)
Published Information
G2571(At1g64380) is part of the BAC clone F15H21, GenBank accession number AC066689 (nid=10645388).
Experimental Observations.
The complete sequence of G2571 was determined and the gene was cloned from cDNA for overexpression. The function of the gene was analyzed using transgenic plants in which G2571 was expressed under the control of the 35S promoter. Overexpression of G2571 resulted reduced size, changes in coloration, branching patterns, and leaf and flower development. In particular, some of the lines showed a sympodial-like growth pattern in the inflorescence, similar to that shown by tomato plants. Thus, the gene could have a key role in regulating shoot meristem activity and branching patterns. G2571 overexpressing lines behaved similarly to the wild-type controls in all physiological assays performed.
Utilities
The alterations in shoot architecture seen in 35S::G2571 lines indicate that the gene might be used to manipulate inflorescence branching patterns. This could influence yield and could offer the potential for more effective harvesting techniques. For instance, the self pruning mutation of tomato results in a determinate growth pattern and facilitates mechanical harvesting (Pnueli et al. (1998) Plant Cell 13: 2687–2702.
G2579 (SEQ ID NO: 451)
Published Information
G2579 was identified as part of the clone T28N17 (GenBank accession AC069328).
Experimental Observations
The function of G2579 was analyzed using transgenic plants in which a cDNA clone of the gene was expressed under the control of the 35S promoter. Overexpression of G2579 produced striking changes in leaf shape and flower development. 35S::G2579 transformants were rather small in stature and formed narrow curled leaves with short petioles. The flowers from these plants exhibited markedly wider carpels than those of wild type, which gave rise to somewhat stumpy club-like siliques. Interestingly, although the fertility of these lines was very poor, siliques appeared to grow out fairly extensively in many instances, indicating that the gene might be producing parthenocarpic effects (fruit development in the absence of seed set).
It is perhaps noteworthy that overexpression of G2579 produced similar effects on carpels to overexpression of another member of the AP2 family, G11796.
Additionally, one of three 35S::G2579 lines examined in physiology assays showed increased tolerance to chilling in a plate-based growth assay.
Utilities
Based on the morphological phenotypes associated with the overexpression of G2579, the gene or its equivalogs could be used to engineer plants with altered fertility or altered fruit size and shape. For example, the parthenocarpic effects that were apparently induced indicated that the gene or its equivalogs might be used to aid the production of seedless fruit varieties.
The results of physiological assays indicate that G2579 or its equivalogs could also be used to generate crop plants that have increased abiotic stress tolerance, and in particular, better growth under cold conditions.
G2585 (SEQ ID NO: 453)
Published Information
G2585 corresponds to gene At5g01900, and it has also been described as WRKY62. No information is available about the function(s) of G2585.
Experimental Observations
The complete sequence of G2585 was experimentally determined. The function of the gene was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Seeds from four out of eighteen of the 35S::G2585 primary transformants were larger than those of control plants grown in the same flats. However, in all other respects, G2585 overexpression lines appeared wild-type.
Utilities
G2585 could be used to alter seed size, shape and composition resulting in higher yielding crop plants.
G2617 (SEQ ID NO: 467)
Published Information
G2617 (At5g06070) was identified in the sequence of TAC clone K16F4 (GenBank accession number AP002030) based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about the function of G2617.
Experimental Observations
The function of G2617 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter.
Overexpression of G2617 severely affected plant growth and development and produced changes in seedling growth rate, rosette phyllotaxy, leaf and flower morphology, overall plant size, and seed yield. At early stages, many of the 35S::G2617 T1 plants appeared to develop faster and seemed more advanced than wild-type, but later became small and stunted. However, this effect on seedling development was not observed in the T2 lines.
Utilities
The developmental effects on G2617 overexpression indicated that the gene might be used to modify growth rate and architecture. Accelerated seedling growth would allow a crop to become established faster. This would minimize exposure to stress conditions at early stages of growth, when the plants are most sensitive. Additionally, it might allow a crop to grow faster than competing weed species. Furthermore, the changes in silique shape displayed by the 35S::G2617 plants, indicated that the gene may be used to alter fruit traits.
G2650 (SEQ ID NO: 483)
Published Information
G2650 is in the sequence of BAC T22D6, GenBank accession number AL357612, released by the Arabidopsis Genome Initiative. No information regarding the function of G2650 is available. Experimental Observations.
The full-length cDNA sequence of G2650 was determined, after which the function of this gene was analyzed in transgenic plants in which G2650 was expressed under the control of the 35S promoter. Plants overexpressing G2650 showed a number of effects that indicate that the gene can influence light regulated developmental processes. 35S::G2650 plants displayed long hypocotyls, elongated petioles, and formed narrow leaves that were held in a more upright orientation than those of controls. About half of the T1 lines and all of the T2 lines examined flowered earlier than wild type. Under 12-hour conditions, the T2 plants developed excessive numbers of small axillary rosette leaves, which may eventually result in an overall increase in biomass. G2650 overexpressing lines also showed enhanced tolerance to chilling stress in a growth assay.
Utilities
Based on the overexpression phenotypes, G2650 could have a number of applications.
(1) Manipulation of light regulated development:
G2650 could influence shade avoidance. Eliminating shading responses might allow increased planting densities with subsequent yield enhancement.
(2) The gene might also be useful in manipulating flowering time. In particular, G2650 could be applied to accelerate flowering or eliminate any requirements for vernalization.
(3) Enhanced chilling stress tolerance.
(4) Altered meristem activity. The increased activity of axillary meristems in the rosettes of 35S::G2650 plants indicates that the gene might be used to increase leaf numbers and biomass. Alternatively, the gene might be used to modulate branching patterns.
G2661 (SEQ ID NO: 487)
Published Information
The sequence of G2661 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AL161746, nid=7327833, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
The function of this gene was analyzed using transgenic plants in which G2661 was expressed under the control of the 35S promoter. G2661 overexpressors showed greener cotyledons on media containing high glucose compared to wild-type controls. This result indicated that G2661 may be involved in sugar sensing. Plants overexpressing G2661 were also found to be slightly smaller, darker, and slower developing than control plants in a small number of transgenic lines.
Utilities
The sugar sensing phenotype of G2661 indicated that this gene may be useful for altering source-sink relationships or other sugar regulated processes.
G2691 (SEQ ID NO: 497)
Published Information
G2691 (At1g25470) was identified as part of the BAC clone F2J7, GenBank accession number AC079281 (nid=10092314).
Experimental Observations
The complete sequence of G2691 was determined. The gene was cloned from cDNA for overexpression. The function of the gene was analyzed using transgenic plants in which G2691 was expressed under the control of the 35S promoter. G2691 overexpressing lines were morphologically wild-type. One out of three lines overexpressing G2691 showed increased seedling vigor (manifested by increased expansion of the cotyledons) in germination assays on high salt compared to wild-type controls.
Utilities
G2691 could potentially be used to increase or facilitate seed germination and seedling growth under adverse environmental conditions, in particular salt stress.
G2694 (SEQ ID NO: 499)
Published Information
G2694 (At3g35770) belongs to a set of transcription factors termed OTHER. This group is comprised of regulatory genes that have been shown to bind DNA in a sequence specific manner and/or are presumed to be transcription factors based on their biochemical properties. In addition, the genes in this category do not belong to an established gene family and are, in effect, singletons in the genome. G2694 corresponds to STERILE APETALA (SAP) that was identified by Byzova et al. ((1999) Genes Dev. 13: 1002–1014). A mutation in SAP in Arabidopsis affects several aspects of reproductive growth including inflorescence, floral organ and ovule development. Mutants in the SAP gene fail to make female gametophytic tissue, and megasporogenesis arrests after the first meiotic division. The internode distance in sap mutants is also reduced, petals are narrow, stamens are short and malformed, and petals and stamens are reduced in number. SAP genetically interacts with both AG and AP2 during inflorescence development. While SAP seems to function synergistically with AP2, the authors hypothesize that SAP is a negative regulator of AG (Byzova et al. (1999) supra).
Experimental Observations
The function of this gene was analyzed using transgenic plants in which G2694 was expressed under the control of the 35S promoter. Overexpression of G2694 produced very striking alterations in seedling size, leaf shape, delayed flowering, and changes in inflorescence and flower morphology. Some of the effects observed in the inflorescence were somewhat similar to those seen in sap mutants by Byzova et al. (1999) supra. However, the effects observed during the vegetative phase were not described for sap. No additional phenotypes were observed in physiological assays.
At the earliest stages, 35S::G2694 seedlings appeared larger than wild type. About two weeks after planting, the seedlings displayed markedly long petioles, narrow leaf blades, and had leaves held in a more vertical orientation than in wild type. Such effects indicated that the gene influences light-regulated developmental programs. Leaves were slightly dark in color and developed an extremely curled and twisted morphology as they expanded. In addition to these effects, the plants produced visible flower buds approximately 5 days later than controls under continuous light conditions. Following the switch to flowering, 35S::G2694 transformants formed inflorescences that had a very leafy appearance; typically an increased number of coflorescence nodes, and a higher order of branching was apparent. Changes in flower morphology were also seen; sepals were frequently enlarged and bract-like, petals and stamens were somewhat contorted, pollen production was low, and carpels were wider than in wild type. Such abnormal flowers were of low fertility and fewer siliques set than in control plants. However, siliques that did develop had a wide flattened appearance.
The morphological effects observed in overexpression lines fit with the hypothesis that SAP interacts with genes such as AGAMOUS, APETALA2 and CURLY LEAF. However, given the pleiotropy of the phenotype, such interactions are likely to be complex, and clearly affect many aspects of plant development.
Utilities
Based on the morphological effects of G2694 overexpression, a wide range of potential commercial applications for the gene or its orthologs exist, including:
(1) Modification of light regulated developmental processes such as shade avoidance.
(2) Modification of vegetative growth and flowering time.
The late flowering and excessive vegetative growth shown by 35S::G2694 lines indicated that the gene or its orthologs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
That 35S::G2694 seedlings were larger than wild type at early stages, indicated that the gene could be used to confer a growth advantage at early stages and allow a crop to attain more rapid ground cover. Additionally, if the slightly dark coloration of 35S::G2694 plants reflects enhanced chlorophyll and/or carotenoid levels, this could enhance photosynthetic capacity and thereby lead to yield improvements. Finally, the changes in leaf shape seen in transgenic lines indicated that the gene could be used to produce novel ornamental forms.
(3) Modification of flower and fruit structure
The dark coloration of 35S::G2694 lines may reflect an increase in biochemical composition, and thus G2694 or its orthologs may be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G2717 (SEQ ID NO: 505)
Published Information
G2717 corresponds to gene At1g49950, and it has also been described as Telomere Repeat Binding Factor 1 (TRBF1). No information is available about the function(s) of G2717.
Experimental Observations
The function of the gene was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2717 lines were wild type with respect to their morphology and development. However, the G2717 overexpressors appeared to be more tolerant to osmotic stress in germination assays. Seedlings from all three transgenic lines were larger than wild-type seedlings at the same developmental stage on control media.
In a soil based drought assay, G2717 overexpressing plants were significantly larger and greener than wild-type control plants.
Utilities
Based on the increased salt, osmotic stress and drought tolerance exhibited by the 35S::G2717 plants in physiology assays, this gene or its equivalogs may be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
Since 35S::G2717 seedlings were slightly larger than controls, the gene or its equivalogs could also be used to accelerate the rate of germination and growth of plants.
G2718 (SEQ ID NO: 507)
Published Information
G2718 (AT1G01380) was identified in the BAC clone, F6F3 (GenBank accession AC023628). Two highly related genes, TRY and CPC have been implicated in epidermal cell specification. A lateral inhibition model proposes that TRY (G1816) and CPC (G225) function as repressors of trichome and atrichoblast cell fate (Shellmann et al. (2002) EMBO J. 21: 5036–5046). A comprehensive review on epidermal cell-fate specification has been published recently (Schiefelbein (2003) Curr. Opin. Plant Biol. 6: 74–78).
Experimental Observations
The function of G2718 was studied using plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2718 resulted in a glabrous phenotype. The effect was highly penetrant, being observed in all primary transformants and each of three independent T2 lines. All of the T1 lines showed a very strong phenotype and completely lacked trichomes on leaves and stems. A comparably severe effect was observed in one of the three T2 populations, whereas the other two T2 populations each exhibited a weaker phenotype, indicating that the effect might have become partially silenced between the generations. Trichomes were present in these weaker lines, but at a much lower density than in wild type.
In addition to the effects on trichome density, 35S::G2718 transformants were also generally slightly smaller than wild type controls.
The phenotypic effects above were observed in the 35S::G2718 as well as in all 35S lines from members of the G2718 clade (G225, G226, G1816, and G682). Similarly, 35S::TF lines from the G2718 clade all had increased root hair formation, reduced anthocyanin levels, and showed improved growth under nitrogen limiting conditions, indicating that the genes improve nutrient uptake. It should be noted however, that due to the apparent silencing of the transgene in the T2 generation, only two of three 35S::G2718 lines examined displayed these phenotypes.
Utilities
The phenotypic effects of G2718 overexpression, such as the increase in root hair formation and the increase in seedling vigor observed in a root growth assay on N-limiting media, indicates that the gene or its equivalogs could be used to engineer plants with increased tolerance to abiotic stresses such as nutrient limitation, drought, salt, heat or cold.
The enhanced performance of G2718 overexpression lines under low nitrogen conditions indicates that the gene or its equivalogs could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
G2718 or its equivalogs could also be used to alter anthocyanin production or trichome formation and production of secondary biochemicals (e.g., lipophilic terpenes) by trichomes.
G2723 (SEQ ID NO: 509)
Published Information
G2723 is a member of the Myb-related family of transcription factors. The gene corresponds to gene At1g19490, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G2723.
Experimental Observations
The complete sequence of G2723 was confirmed, and the function of the gene was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G2723 plants did not show consistent alterations in their response to the physiological analyses that were performed.
With respect to the morphology and development of the transgenic plants, overexpression of G2723 produced a moderate delay in the onset of flowering in Arabidopsis by up to approximately two weeks under continuous light conditions. The phenotype was apparent in eleven out of eighteen of the primary transformants and each of three T2 populations that were examined.
Utilities
The delayed flowering displayed by 35S::G2723 transformants indicates that the gene or its orthologs can be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases
Given the effects of G2723 overexpression, it is likely that the activity of the gene or its orthologs can be modified to accelerate flowering or eliminate any requirement for vernalization.
G2741 (SEQ ID NO: 511)
Published Information
G2741 was identified in the sequence of BAC F12A12, GenBank accession number AL133314, released by the Arabidopsis Genome Initiative. No functional information is available about G2741.
Experimental Observations
The function of G2741 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Five of the eighteen 35S::G2741 lines were significantly delayed in flowering and exhibited greater vegetative biomass than wild-type. No altered phenotypes were detected in any of the physiological assays.
It should be noted that G2741 is closely related to G1435, which also produced late flowering plants when overexpressed.
Utilities
The delayed flowering displayed by 35S::G2741 transformants indicated that the gene or its equivalogs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases. Given the effects of G2741 overexpression, it is possible that the activity of the gene or its equivalogs could be modified to accelerate flowering, or eliminate any requirement for vernalization.
G2754 (SEQ ID NO: 517)
Published Information
The transcription regulator G2754 was identified by amino acid sequence similarity to proteins of the SWI/SNF family of chromatin remodeling factors. G2754 is found in the sequence of the chromosome 5, P1 clone MSN2 (GenBank accession number AC005936.1; nid=3702737), released by the Arabidopsis Genome Initiative. No additional public information related to the functional characterization of G2754 is available.
Experimental Observations
The function of G2754 was analyzed through its ectopic overexpression in Arabidopsis; 35S::G2754 seedlings were slightly pale in coloration, displayed long hypocotyls, elongated petioles, and had leaves held in a more upright orientation. Many of the lines also flowered noticeably earlier than controls. Following the switch to flowering, the inflorescences from 35S::G2754 plants had a spindly appearance and exhibited somewhat increased internode elongation compared to wild type. The above effects were observed in ten out of eighteen of the primary transformants and each of three independent T2 lines.
Utilities
The phenotype of plants overexpressing G2754 indicated that the gene or its orthologs might be used to manipulate developmental processes regulated by light, such as shade avoidance. Eliminating shading responses could lead to increased planting densities with subsequent yield enhancement.
The gene or its orthologs might also be useful in modifying flowering time. In particular, G2754 could be applied to accelerate flowering or eliminate any requirements for vernalization.
G2763 (SEQ ID NO: 521)
Published Information
The sequence of G2763 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AB026636, nid=4757392, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
The 5′ and 3′ ends of G2763 were experimentally determined by RACE. The full-length cDNA clone corresponding to G2763 was isolated in-house from the screening of Arabidopsis cDNA libraries.
The function of G2763 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from the 35S CaMV promoter. Overexpression of G2763 resulted in plants that displayed a mild delay in the onset of flowering, compared to wild-type controls. Additionally, a number of the plants were slightly small and appeared dark in coloration, particularly at later stages of development. In the physiological assays, G2763 overexpressors have a sugar sensing phenotype in a germination assay on media containing high glucose. The small seedling phenotype was confirmed in a repeat experiment on all three individual line. In addition, all three lines of G2763 were more sensitive to chilling stress in the chilling growth assay. Seedlings were small and in the case of line 5 had more anthocyanin accumulation.
The sugar sensing phenotype of G2763 overexpressing plants may be related to the late flowering phenotype. Sugars are central regulatory molecules that control several aspects of plant physiology, metabolism, and development, including flowering.
Utilities
The delayed flowering displayed by 35S::G2763 transformants indicates that the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
G2763 might be used to generate crop plants with altered sugar sensing.
G2763 could also be used to generate crop plants enhanced resistance to chilling.
If the dark coloration of 35S::G2763 lines reflects an increase in biochemical composition, the gene might be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G2768 (SEQ ID NO: 525 and SEQ ID NO: 2118)
Published Information
The sequence of G2768 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AB018117 (nid=3702735). It corresponds to At5g47430, annotated by the Arabidopsis Genome Initiative.
Experimental Observations
The function of G2768 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from the 35S CaMV promoter.
The G2768 clone within the overexpression construct (P15431; comprising SEQ ID NO: 2118) encodes a product that lacks 227 amino acids at the carboxy-terminus compared to the full-length wild-type protein. Thus, it is possible that the morphological phenotypes seen in plants transformed with P15431 represent dominant negative type effects.
Overexpression of G2768 produced dramatic changes in flower morphology; flowers from 35S::G2768 transformants were strikingly similar to those of mutants for the MADS-box gene, AGAMOUS (AG, Bowman et al. (1989) Plant Cell 1: 37–52; Bowman et al. (1991a) Development 112: 1–20; Yanofsky et al. (1990) Nature 346: 35–39; Coen and Meyerowitz (1991) Nature 353: 31–37).
In a typical 35S::G2768 flower, a general loss of determinate floral meristem growth was apparent, such that a new flower bud developed within, or in place of, the central carpels. This pattern was then reiterated and the fourth whorl of the secondary flower in turn gave rise to a tertiary flower bud and so on, resulting in a chain of flowers, each emerging from the center of the previous one. The phenotype was observed to varying extents, and in the most severe cases, a central carpel was converted completely into a new flower bud. In other instances, however, the conversion was incomplete; fourth whorl organs developed as a contorted pair of carpel-like structures, which when dissected open, were seen to contain the vestigial floral organs of a secondary flower. Additionally, stamen development was compromised, and in the severest cases, six petals developed in place of stamens in the third whorls. As a result of these changes, 35S::G2768 flowers were infertile and failed to yield seed.
The features described above are identical to those displayed by AG mutants, indicating that G2768 might interact with AG during the development of wild type flowers. In particular, AG is specifically expressed in the third and fourth whorls of developing flowers, where it specifies stamen and carpel identity respectively, and prevents indeterminate growth of the floral meristem (Bowman et al. (1991b) Plant Cell 3: 749–758; Drews et al. (1991) Cell 65: 991–1002): Thus, in wild-type plants, G2768 might have a role in preventing AG expression in first and second floral whorls.
Additionally, G2768 overexpression produced changes in leaf shape, such that those organs became larger and flatter than in wild type.
Utilities
Based on the effects of G2768 overexpression, the gene or its equivalogs could be used to manipulate flower structure and development. A wide range of applications could be envisaged, including the following:
(1) production of larger showier flowers for the ornamental market; (2) the gene or its equivalogs might be used to engineer sterility in trees and grasses to prevent escape of pollen from genetically engineered plants; (3) the sterility of the 35S::G2768 flowers, and failure of seed set, delays senescence, which would increase duration of the flowering period in ornamentals; (4) the gene or its equivalogs could be applied to artichokes to eliminate the choke; (5) the gene or its equivalogs could be applied to roses; increased numbers of petals might be of interest to the rose oil industry.

Given the increase in leaf size observed in 35S::G2768 lines, the gene or its equivalogs may also be applied to increase biomass and yield in crop plants.
G2771 (SEQ ID NO: 527 and SEQ ID NO: 2119)
Published Information
G2771 corresponds to gene AT4G28810, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G2771.
Experimental Observations
The function of G2771 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2771 produced striking alterations in leaf morphology and a marked delay in the onset of flowering. The leaves of 35S::G2771 lines were noticeably narrower, darker, and more curled than were those of controls, particularly at late stages. At early stages, plants grown on plates were observed to show long hypocotyls and were rather pale in coloration, indicating that G2771 might influence light-regulated development. These lines also showed reduced accumulation of anthocyanins when subjected to a chilling growth assays.
It should be noted that the G2771 overexpression construct, contained a truncated clone of the gene (SEQ ID NO: 2119); the above phenotypes might therefore represent dominant negative effects.
Utilities
Based on the morphological effects of overexpression, G2771 or its equivalogs might be used to manipulate leaf shape. In particular, this could be used to create novel forms for the ornamental plant market. This effect might also have other commercial applications. Increased leaf size, or an extended period of leaf growth, could increase photosynthetic capacity, and biomass, and have a positive effect on yield. The dark coloration observed in 35S::G2771 transformants point towards a number of utilities. Consumption of dark green leafy vegetables has been shown in clinical studies to reduce the risk of ARMD.
Additionally, the delayed flowering displayed by 35S::G2771 transformants indicated that the gene or its equivalogs might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
G2771 or its equivalogs could also have a role in modulating developmental processes regulated by light, such as shade avoidance. Eliminating shading responses could allow for increased planting densities with subsequent yield enhancement.
G2771 or its equivalogs might also be used to generate crop plants that have better growth under cold conditions. The growth of many crops is very sensitive to cool temperatures. A gene that enhances growth under chilling conditions could result in enhanced yields.
G2776 (SEQ ID NO: 529)
Published Information
The sequence of G2776 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AL161592, nid=7270751, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
The function of G2776 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from the 35S CaMV promoter. G2776 overexpressors have a sugar sensing or osmotic tolerant phenotype in a germination assay on media containing high sucrose. Seedlings of 35S::G2776 transgenic lines were larger with green cotyledons compared with wild-type seedlings grown on sucrose. The result could indicate that G2776 may be involved in sugar sensing and/or osmotic stress tolerance.
Utilities
The sugar sensing phenotype of G2776 indicates that this gene may be useful for altering source-sink relationships or other sugar regulated processes.
If the phenotype of 35S::G2776 seedlings on sucrose plates reflects a general resistance to osmotic stress conditions, the gene might be used to engineer plants that are more resilient to abiotic stresses such as drought, salt and/or freezing.
G2784 (SEQ ID NO: 537 and SEQ ID NO: 2120)
Published Information
G2784 corresponds to gene AT5G57770, annotated by the Arabidopsis Genome Initiative.
Experimental Observations
The 5′ and 3′ ends of G2784 were determined by RACE. The function of G2784 was analyzed using transgenic plants in which G2784 was expressed under the control of the 35S promoter.
Overexpression of G2784 (SEQ ID NO: 2120) produced marked changes in overall plant architecture. 35S::G2784 transformants, were small, slow developing and exhibited curled dark leaves. Additionally, plants from two of the T2 lines exhibited abnormal inflorescence morphology and produced secondary shoots that grew downwards.
35S::G2784 lines showed more tolerance to cold stress in a germination assay. When the assay was repeated on individual lines, all three lines showed enhanced seeding vigor when germinated under cold conditions.
Utilities
G2784 or its equivalogs could be used to engineer enhanced cold germination in plants. The germination of many crops is very sensitive to cold temperatures. A gene that would enhance germination and seedling vigor in the cold would have tremendous utility in allowing seeds to be planted earlier in the season with a higher survival rate.
The morphological changes exhibited by 35S::G2784 plants indicated that the gene or its equivalogs might be used to manipulate plant architecture. In particular, G2784 could be applied to produce novel leaf and shoot morphologies for the ornamental markets.
Additionally, if the altered coloration of 35S::G2784 plants reflects a change in biochemical composition, the gene or its equivalogs may be used to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity to improve yield.
G2826 (SEQ ID NO: 545)
Published Information
G2826 (At1g68360) is in BAC T22E19 (GenBank accession number AC016447) and was identified based on its sequence similarity within the conserved domain to other zinc finger C2H2-related proteins in Arabidopsis . There is no published or public information about the function of G2826.
Experimental Observations
The function of G2826 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Few primary transformants were produced, indicating that the gene can be lethal when overexpressed at high levels. Overexpression of G2826 resulted in plants that were small and slow developing. Some individuals developed aerial rosettes at coflorescence nodes, indicating a disruption in phase change in the inflorescence. Flowers were also small and had defects in organ formation, pollen production, and yielded few seeds. Strikingly, flowers also displayed increased trichome density on sepals and often possessed ectopic trichomes on the carpels. On dissecting the carpels, trichome-like structures developing from the internal walls were also apparent. Some plants also had floral organs converted towards a bract-like identity. The changes in morphology produced by overexpression of this gene is indicative of heterochronic shifts (i.e., cells in various lineages and tissues adopt fates that are normally associated with cells from other developmental stages). For example, trichomes are normally associated with vegetative rather than reproductive organs. Additionally, aerial rosettes occur when a secondary inflorescence meristem develops in a manner comparable to a primary shoot meristem during the vegetative phase of growth.
Utilities
The morphological effects of G2826 overexpression indicate a number of potential applications relating to increasing or changing trichome density: Thus, the use of G2826 and its homologs to increase trichome density, size or type may therefore have profound utilities in so called molecular farming practices and increasing the yield of cotton fibers.
If the effects on trichome patterning and/or aerial rosette formation reflect a general change in heterochronic processes, G2826 or other clade members might be used to modify the way meristems and/or cells develop during different phases of the plant life cycle. In particular, altering the timing of phase changes could afford positive effects on yield and biomass production.
G2830 (SEQ ID NO: 547)
Published Information
G2830 (At5g42640) was identified in the sequence of P1 clone MFO20, GenBank accession number AB013391, released by the Arabidopsis Genome Initiative. There is no published or public information about the function of G2830.
Experimental Observations
G2830 is expressed at a low level in embryos and siliques as determined by RT-PCR analysis. Expression of G2830 was not detected in other tissues. Previously, a line homozygous for a T-DNA insertion in G2830 was used to determine the function of this gene. G2830 mutant plants showed an increase in seed oil content.
The phenotype of 35S::G2830 lines has now been analyzed. No consistent effects on Arabidopsis growth and development were found in morphological assays. However, a proportion of plants from a single 35S::G2830 T2 line performed better than wild-type plants when germinated on media with low nitrogen supplemented with sucrose or with sucrose plus glutamine.
Utilities
G2830 or its equivalogs may be used to increase seed oil content of plants, which may be used to improve seed oil yield or increase the caloric content of foods.
Because expression of G2830 is embryo and silique specific, its promoter could be useful for targeted gene expression in these tissues. That fact that 35S::G2830 transformants produced less anthocyanin on high sucrose plus glutamine indicated that G2830 might be used to modify carbon and nitrogen status, and hence assimilate partitioning.
G2838 (SEQ ID NO: 555)
Published Information
G2838 was identified in the sequence of BAC F15M7, GenBank accession number AP002543, released by the Arabidopsis Genome Initiative. There is no other published or public information about the function of G2838.
Experimental Observations
The 5′ and 3′ ends of G2838 were determined by RACE PCR. The complete sequence of G2838 was determined. The function of G2838 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Many of the 35S::G2838 seedling transformants had large cotyledons compared to control plants. As plants matured, many lines became rather dark in coloration, showed a delay in the onset of flowering, and an increase in vegetative characteristics in the inflorescence. In some instances, aerial rosettes were seen at coflorescence nodes, and in other cases flowers had shoot like characteristics. Sepals from some flowers had a bract-like appearance and an increase in trichome density. Many lines showed non-specific flower abnormalities; floral organs were often small and contorted and pollen production was poor. As a result, seed yield from many of the lines was rather poor.
Utilities
The morphological effects of G2838 overexpression indicate a number of applications relating to increasing or changing trichome density by modification of the expression of this transcription factor or its equivalogs. Thus, the use of G2838 and its homologs to increase trichome density, size or type may therefore have profound utilities in so-called molecular farming practices and increasing the yield of cotton fibers. Since the mallow family is closely related to the Brassica family in which Arabidopsis is located, genes involved in trichome formation are likely to have homologs that function in cotton.
If the effects on trichome patterning and/or aerial rosette formation reflect a general change in heterochronic processes, G2838 or other clade members, might be used to modify the way meristems and/or cells develop during different phases of the plant life cycle, or manipulate flowering time. In particular, altering the timing of phase changes could afford positive effects on yield and biomass production. The enlarged size of 35S::G2838 seedlings might also be interpreted as a heterochronic shift, and indicated that the gene might be applied to crops in order to expedite ground coverage.
The dark coloration of 35S::G2838 lines might reflect a change in biochemical composition or chlorophyll content. Thus, the gene might be applied to improve the nutraceutical value of foodstuffs, or increase photosynthetic capacity in crop plants, to improve yield.
G2839 (SEQ ID NO: 557)
Published Information
G2839 (At3g46080) was identified in the sequence of BAC F12M12 (GenBank accession number AL355775) based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about the function of G2839.
Experimental Observations
The function of G2839 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Few primary transformants were generated. This indicates that G2839 overexpression can be lethal. T1 lines displayed stunted growth and development, and yielded very few or zero seeds. Inflorescences were poorly developed. In one line, flower pedicels were very short and flowers and siliques were oriented downwards. G2839 overexpressors showed a phenotype in a germination assay on media containing high sucrose: seedlings were green and had high germination rates. Thus, the gene appeared to influence sugar sensing and/or osmotic stress responses.
G2839 is closely related to G354 and G353. Flower phenotypes in which pedicels were very short and flowers and siliques were oriented downwards have been described for G353 and G354 and are also similar to the brevipedicellus mutant (Koornneef et al. (1983) J. Heredity 1983; 74: 265–272; Venglat et al. (2002) Proc. Natl. Acad Sci USA. 99:4730–4735; Douglas et al. (2002) Plant Cell 14:547–558. Interestingly 35S::G353 lines also showed increased resistance to osmotic stress.
Utilities
The phenotypes observed in physiology assays indicate that G2839 might be used to generate crop plants with altered sugar sensing. If the physiological phenotype is related to osmotic stress, the gene could be used to engineer cold and dehydration tolerance.
The morphological phenotype shown by 35S::G2839 lines indicate that the gene might be used to alter inflorescence architecture. In particular, a reduction in pedicel length and a change in the position at which flowers and fruits are held, might influence harvesting or pollination efficiency. Additionally, such changes might produce attractive novel forms for the ornamental markets.
G2854 (SEQ ID NO: 567)
Published Information
The sequence of G2854 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AL161566, nid=7269538, based on its sequence similarity within the conserved domain to other ACBF-like related proteins in Arabidopsis.
Experimental Observations
The 5′ and 3′ ends of G2854 were determined by RACE. The function of G2854 was analyzed using transgenic plants in which G2854 was expressed under the control of the 35S promoter. 35S::G2854 transformants showed increased germination efficiency on sucrose plates compared to wild-type controls.
Utilities
G2854 may be used to generate crop plants with altered sugar sensing.
G2859 (SEQ ID NO: 569)
Published Information
G2859 corresponds to gene AT1G73830, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G2859.
Experimental Observations
The function of G2859 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S:: G2859 transgenic plants produced marked changes in Arabidopsis seedling morphology, coloration, leaf shape, and inflorescence development. 35S::G2859 transformants appeared pale in coloration during all phases of the life cycle. At early stages, the seedlings displayed rather long hypocotyls and long oval shaped cotyledons. Such a phenotype could indicate that G2859 was influencing light regulated developmental programs. Later, the plants formed leaves were rather flat and had mild serrations on the margins. Following the switch to reproductive growth, 35S::G2859 inflorescences became increasingly proliferated and bushy as the plants aged, exhibited very thin stems, long narrow curled cauline leaves, and carried flowers that were rather small and had poorly developed organs.
G2859 overexpressing lines behaved similarly to the wild-type controls in all physiological assays performed.
Utilities
Based on the effects of G2859 overexpression, the gene or its orthologs could be used to modify light-regulated developmental processes such as shade avoidance. Additionally, G2859 could be used to manipulate inflorescence architecture and generate plants with a denser bushier shoot structure.
G2885 (SEQ ID NO: 579)
Published Information
G2885 was identified in the sequence of genomic clone K21L19.7, GenBank accession number AB024029, released by the Arabidopsis Genome Initiative. It is a member of the response regulator class of GARP proteins, and was recently named ARR18 (Hwang et al. (2002) Plant Physiol. 129: 500–515). No functional information is available about G2885.
Experimental Observations
The function of G2885 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2885 produced highly pleiotropic effects on Arabidopsis development, including alterations in meristem initiation and growth, cell differentiation, leaf shape, flower morphology, overall plant size, and rate of senescence. Most strikingly, two T2 lines showed secondary rosettes of leaves developing from the adaxial surface of cotyledons. This phenotype was identical to that seen with overexpression of a truncated ARR1 (G1493) protein lacking its response regulator domain. Callus-like outgrowths were also noted on the stems of two T1 plants, consistent with the observation of disordered cell proliferation in the plants overexpressing a truncated ARR1 (Sakai et al. (2001) Science 294: 1519–1521). ARR1 is known to function in cytokinin signal transduction, and G2885 is therefore also likely to function in cytokinin response. G2885 may be a stronger activator than ARR1, since the ectopic rosettes did not appear in plants expressing an intact ARR1 protein (Sakai et al. (2001) supra; G1493). In physiology assays, 35S::G2885 lines were more sensitive to cold conditions than wild-type plants, indicating that the gene can influence the response to abiotic stress.
Utilities
The overexpression phenotypes of G2885 indicated that the gene regulates meristem activity and stem cell identity. As such, the gene or its orthologs could have applications in cell cultures lines, or in transformation or micro-propagation systems, where regeneration of shoots from callus is currently problematic.
Based on the increased sensitivity to cold exhibited by the 35S::G2885 lines, this gene or its orthologs could be used to engineer cold tolerance. The germination of many crops is very sensitive to cold temperatures. A gene that altered sensitivity to cold would have utility in understanding the sensory transduction pathway for the regulation of growth and development by temperature. Understanding how plants respond to temperature could lead to plants with enhanced germination and seedling vigor in the cold and have utility in allowing seeds to be planted earlier in the growing season with a higher survival rate.
G2907 (SEQ ID NO: 587 and SEQ ID NO: 2122)
Published Information
G2907 has been described as AtSR1 ( Arabidopsis thaliana Signal-Responsive genes; Yang and Poovaiah (2002) J. Biol. Chem. 277: 45049–45058). The authors have shown that the protein contains a functional calmodulin-binding domain in addition to the DNA binding domain. AtSR1 specifically recognizes a novel 6-bp motif (CGCC box). Expression analysis indicates that AtSR1 transcript levels increase in response to different signals and stresses such as ethylene, methyl jasmonate, hydrogen peroxide, heat, cold, UV light and sodium chloride (Yang and Poovaiah (2002) supra). G2907 also corresponds to sequence 2286 from patent publication WO0216655 A2 (2001) on stress-regulated genes, transgenic plants and methods of use. No functional characterization in planta has been published so far.
Experimental Observations
The function of G2907 was analyzed using transgenic plants in which a cDNA clone of the gene was expressed under the control of the 35S promoter. Overexpression of G2907 (SEQ ID NO: 2122) markedly accelerated the onset of leaf senescence in Arabidopsis . This phenotype was apparent in three independent 35S::G2907 T2 lines, and was confirmed when the lines were re-grown for a second time. For unknown reasons, however, the phenotype was not noted in the T1 generation.
35S::G2907 plants were indistinguishable from wild-type controls in all physiology assays performed.
Utilities
G2907 or its equivalogs may have utility in altering senescence-related processes. Although leaf senescence is thought to be an evolutionary adaptation to recycle nutrients, the ability to control senescence in an agricultural setting may have value. For example, a delay in leaf senescence in some maize hybrids is associated with a significant increase in yields and a delay of a few days in the senescence of soybean plants can have a large impact on yield as well. Delayed flower senescence may also generate plants that retain their blossoms longer, this could impact yield and may be of potential interest to the ornamental horticulture industry.
G2913 (SEQ ID NO: 589)
Published Information
G2913 (At1g76110) was identified as part of the BAC clone T23E18 (GenBank accession AC009978).
Experimental Observations
The function of G2913 was analyzed using transgenic plants in which a cDNA clone of the gene was expressed under the control of the 35S promoter. In an assay intended to determine whether the transgene expression could alter carbon and nitrogen sensing, 35S::G2913 seedlings contained less anthocyanins (and in some cases were larger) than wild-type controls grown on high sucrose/N-plates. The transgenic seedlings were also greener on high sucrose/N-/Gln plates.
Utilities
The enhanced performance of G2913 overexpression lines under low nitrogen conditions indicated that the gene or its orthologs can be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
G2930 (SEQ ID NO: 591)
Published Information
The sequence of G2930 was obtained from the Arabidopsis genome sequencing project, GenBank accession number AC016972, nid=6714311, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
The complete sequence of G2930 was determined experimentally. The function of G2930 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G2930 resulted in plants that were more tolerant to chilling stress in a growth assay compared to control plants. However, overexpression of G2930 produced no consistent effects on Arabidopsis morphology.
Utilities
G2930 could be used to generate crop plants that are more tolerant to chilling stress.
G2933 (SEQ ID NO: 593)
Published Information
The sequence of G2933 was obtained from Arabidopsis genomic sequencing project, GenBank accession number AL138655, nid=6899905, based on its sequence similarity within the conserved domain to other bHLH related proteins in Arabidopsis.
Experimental Observations
The function of G2933 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from the 35S CaMV promoter. A small number of G2933 overexpression lines produced larger seeds than wild-type controls. The result indicates that G2933 is involved in the regulation of sink-source relationship in plants. In addition, seedlings of 35S::G2933 transgenic lines showed more tolerance to chilling stress in a growth assay. When the assay was repeated on individual lines, all three lines analyzed showed the phenotype.
Utilities
G2933 might be used to modify sink-source relationship and thereby enhance seed yield.
This gene could also be used to generate crop plants that have better growth under cold conditions. The growth of many crops is very sensitive to cool temperatures. A gene that enhances growth under chilling conditions could result in enhanced yields.
G2969 (SEQ ID NO: 603)
Published Information
G2969 (At2g29660) was identified in the sequence of Arabidopsis thaliana chromosome 2 clone T27A16 (GenBank accession number AC005496) based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about the function of G2969.
Experimental Observations
The function of G2969 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. G2969 overexpressing lines showed increased tolerance to sucrose and ABA in germination assays. The ABA and sucrose insensitivity indicates that the effect of overexpressing G2969 might cause tolerance to osmotic stress. 35S::G2969 plants were wild type in morphological analyses that were performed.
Utilities
G2969 appears to affect ABA sensitivity. ABA is one of key signal molecules in the stress response pathways. Therefore, G2969 may have a utility in modifying ABA responses such as seed dormancy, seed development, and cold and drought tolerances.
G2969 might also be used to generate crop plants with altered sugar sensing.
G2972 (SEQ ID NO: 605)
Published Information
G2972 (At3g29340) was identified in the sequence of P1 clone MUO10 (GenBank accession number AP001309) based on its sequence similarity within the conserved domain to other C2H2 related proteins in Arabidopsis . There is no published or public information about G2972.
Experimental Observations
The function of G2972 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. G2972 overexpressing lines showed more tolerance to growth under low phosphate conditions. 35S::G2972 plants were wild type in morphological analyses that were performed.
Utilities
The response of 35S::G2972 seedlings to low phosphate conditions indicates that the gene could be used to manipulate nutrient uptake, or the ability to grow in poor nutrient soils.
G2979 (SEQ ID NO: 607)
Published Information
The transcription factor G2979 was identified by amino acid sequence similarity to the mammalian E2F proteins. It has been referenced in the public literature both as E2L2 and E2Ff (Kosugi and Ohashi, (2002) J. Biol. Chem. 277: 16553–16558; Mariconti et al. (2002) J. Biol. Chem. 277: 9911–9919). G2979 is found in the sequence of the chromosome 3 BAC T22N4 (AC010676.6 GI:1240872), released by the Arabidopsis Genome Initiative. The G2979 product is thought to function as a repressor and be involved in restricting cell proliferation (Kosugi and Ohashi (2002) supra).
Experimental Observations
The function of G2979 was analyzed through its overexpression in Arabidopsis; 35S::G2979 lines displayed a mild delay in the onset of flowering, a marked increase in vegetative biomass, and increases in floral organ number. Its seems more likely that increased floral organ number and leaf size are related effects, and could both be due to a change in meristem activity, such as increased numbers of cells being allocated to organ primordia, or such cells going through additional rounds of cell division.
Utilities
Based on the substantially increased size of 35S::G2979 organs, the gene or its equivalogs could be used to increase plant biomass, thus improving yield. The increased flower size seen in such plants indicated that G2979 or its equivalogs could be applied to produce desirable flower and fruit traits.
Additionally, the slight delay in flowering observed in some of the 35S::G2979 lines indicated that the gene or its equivalogs might be used to manipulate the timing of reproductive growth. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases. Conversely, it is possible that the activity of G2979 or its equivalogs could be modified to accelerate flowering, or eliminate any requirement for vernalization.
G2981 (SEQ ID NO: 609)
Published Information
G2981 is similar in its amino acid sequence to the mammalian DP2a, a dimerization partner to E2F required for the progression and arrest of the cell cycle in animals and plants. G2981 is in chromosome 5, BAC clone F12E4 (GenBank accession AL162751.1 GI:7378607), released by the Arabidopsis Genome Initiative. No public information related to the functional characterization of G2981 is available.
Experimental Observations
The boundaries of G2981 were determined by RACE (Rapid Amplification of cDNA Ends; a PCR-based method that facilitates the cloning of full-length cDNA sequences when a partial cDNA sequence is known) and its function was analyzed through overexpression in Arabidopsis. 35::G2981 seedlings were larger and appeared to have less anthocyanin on plates that were nitrogen deficient, but which were supplemented with glutamine and high sucrose levels. This assay monitors the effect of carbon on nitrogen signaling through anthocyanin production.
Utilities
The enhanced performance of G2981 overexpression lines under low nitrogen conditions indicate that the gene could be used to engineer crops that could thrive under conditions of reduced nitrogen availability.
That 35S::G2981 lines make less anthocyanin on high sucrose plus glutamine, indicates G2981 might be used to modify carbon and nitrogen status, and hence assimilate partitioning.
G2982 (SEQ ID NO: 611)
Published Information
G2982 is found in the sequence of the chromosome 5, BAC clone T22P11 (GenBank accession AL162971.1 GI:7413630), released by the Arabidopsis Genome Initiative. The gene appears to have a role in cell cycle control (Magyar et al. (2000) FEBS Lett. 486:79–87) and its sequence has recently been included in patent publication WO0185946 A2.
Experimental Observations
The function of G2982 was analyzed through overexpression of a genomic clone in Arabidopsis. 35S::G2982 transformants displayed increased tolerance to dehydration stress. In all other respects, these transgenic lines appeared wild type.
In a soil based drought assay, G2982 overexpressing Arabidopsis plants were significantly greener and larger than wild-type control plants.
Utilities
The response of 35S::G2982 plants to dehydration stress indicated that G2982 or its equivalogs could be used to improve plant tolerance to cold, freezing, drought, and salt conditions.
G2983 (SEQ ID NO: 613)
Published Information
G2983 was initially identified within a sequence released by the Arabidopsis genome initiative (gene F2G19.11 within BAC clone F2G19, Chromosome 1, GenBank accession, AC083835), as a gene encoding a novel WUSCHEL-like homeodomain protein. No data are available regarding the function of this locus.
Experimental Observations
The boundaries of G2983 were initially determined by RACE experiments, and transgenic lines were generated in which the gene was overexpressed from a 35S promoter. These plants displayed some striking alterations in morphology compared to wild type. 35S::G2983 lines exhibited a spectrum of developmental changes including alterations in leaf shape, phyllotaxy, coloration, growth rate, floral organ abnormalities, and a reduction in overall size. However, the most prominent phenotype was seen in the inflorescence, where strange growths developed from stems, pedicels and floral organs. In some cases, such outgrowths showed stigmatic tissue or took on a trichome-like identity.
Similar results from overexpression of a related gene, WUSCHEL had previously been obtained, in which the latter gene was found to induce the formation of callus like outgrowths. WUSCHEL has a key role in the maintenance of stem cell identity within apical meristems, and during the reproductive phase, participates in a feedback loop with the AGAMOUS gene, which induces floral meristems to terminally differentiate into carpels (Mayer et al. (1998) Cell 95: 805–815; Schoof et al (2000) Cell 100: 635–644; Lohmann et al. (2001) Cell 105: 793–803). The similarity between the WUS and G2983 overexpression phenotypes indicated that the genes might have similar roles in regulating apical meristem activity. Two other WUS-like genes, G1539 and G1591, have also yielded similar effects on the inflorescence to G2983.
An additional, potentially related phenotype was observed in the roots of 35S::G2983 lines in physiology experiments. During assays which involved the monitoring of root growth on vertical plates, following inversion of plates, it was noted that 35S::G2983 roots displayed an abnormal gravitropic response; rather than growing downwards, the roots grew in a spiral pattern, and appeared to proliferate and generate an increased number of root hairs.
Utilities
The overexpression phenotypes of G2983 indicated that this transcription factor or its orthologs might be used to regulate meristem activity and stem cell identity. As such, the gene could have applications in the plant cell culture lines, or in transformation or micro-propagation systems, where generation of callus is currently problematic but is required as part of the procedure. Additionally, the effects on root morphology seen in 35S::G2983 plants, indicated that the gene might be used to manipulate root hair development and thereby enhance the ability of crops to survive abiotic stresses such as drought. Finally, the alterations in trichome development seen in occasional lines indicated that the gene could be used to manipulate the formation of those structures.
Given its potential capacity to trigger ectopic carpel development in Arabidopsis , G2983 or its orthologs can be applied to commercial species to induce formation of increased numbers of carpels or fruits. A particular application might exist in saffron, one of the world's most expensive spices. Saffron filaments, or threads, are actually the dried stigmas of the saffron flower, Crocus Sativus Linneaus. Each flower contains only three stigmas, and more than 75,000 of these flowers are needed to produce just one pound of saffron filaments. A gene such as G2983, which increased carpel numbers, could therefore substantially increase yield.
G2990 (SEQ ID NO: 615)
Published Information
G2990 corresponds to gene MKM21.8 within P1 clone MKM21 (GenBank accession AB016S76) derived from chromosome 5. We identified this locus as a novel member of the ZF-HB family and no data regarding its function are currently in the public domain (as of Aug. 5, 2002).
Experimental Observations
The boundaries of G2990 were identified by RACE experiments performed and a full-length clone was then PCR-amplified from cDNA derived from mixed tissue samples. Full-length cDNA sequences for this gene have recently been deposited in GenBank (Accessions AY091034 and AY117347), and the coding sequences are identical to that identified by us.
The function of G2990 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from a 35S CaMV promoter. Under normal growth circumstances, 35S::G2990 transformants displayed wild-type morphology. However, two of three independent T2 populations showed an altered response to nitrogen deprivation in plate-based assays, indicating that the gene might be involved in the response to conditions of nutrient limitation.
Utilities
The data from physiological assays, revealing that G2990 can influence the response to nitrogen deprivation, indicate that the gene might have utility in engineering commercial species that can be successfully cultivated in low nitrogen soils or growth media.
G2992 (SEQ ID NO: 617)
Published Information
G2992 corresponds to gene F24J1.29 within BAC clone F24J1 (GenBank accession AC021046) derived from chromosome 1. No data regarding its function are currently in the public domain.
Experimental Observations
This locus was identified as a member of the ZF-HB family. The boundaries of G2992 were determined by RACE, and a clone was PCR-amplified from cDNA derived from mixed tissue samples. The function of G2992 was then assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from a 35S CaMV promoter. 35S::G2992 T2 populations displayed an enhanced ability to germinate on plates containing high levels of sodium chloride, and on plates containing high levels of ABA. Thus, G2992 can function as part of response pathway to abiotic stress. 35S::G2992 seedlings were also noted to be rather pale in coloration, and appeared more sensitive than wild type to conditions of nitrogen deprivation. Furthermore, 35S::G2992 seedlings also showed altered root morphology; fewer lateral roots were present. Additionally, morphological studies revealed that overexpression of G2992 can accelerate the onset of reproductive development, reduce plant size, and produce changes in leaf shape.
Utilities
Based on the phenotypes observed in morphological and physiological assays, G2992 might be have a number of applications.
Given the salt resistance and ABA insensitivity exhibited by 35S::G2992 transformants, the gene might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
G2992 appears to affect ABA sensitivity; therefore the gene may have a utility in modifying ABA responses such as seed development and dormancy, as well as cold and dehydration tolerance.
The data from physiological assays, revealing that G2992 can influence the response to nitrogen deprivation, indicate that the gene might have utility in engineering commercial species that can be successfully cultivated in low nitrogen soils or growth media.
The early flowering exhibited by 35S::G2992 lines, indicates that the gene might be used to manipulate flowering time in commercial species. In particular, G2992 could be applied to accelerate flowering or eliminate any requirements for vernalization.
Finally, the effects of G2992 overexpression on leaf shape indicate that the gene might be used to modify plant architecture.
G2996 (SEQ ID NO: 621)
Published Information
No data regarding the function of this gene are presently known or available.
Experimental Observations.
This locus was identified as a novel member of the ZF-HB family. The boundaries of G2996 were identified from partial EST sequences in the public databases and were confirmed by RACE experiments. A full-length clone was then PCR-amplified from cDNA derived from mixed tissue samples. The function of G2996 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from a 35S CaMV promoter. Under normal growth conditions, 35S::G2996 transformants displayed wild-type morphology. However, each of three independent T2 populations showed increased sensitivity to mannitol in plate-based root growth inhibition assays, indicating that G2996 can influence osmotic stress responses.
Utilities
The data from physiological assays, revealing that G2996 can influence osmotic stress responses, indicate that the gene might have utility in engineering commercial species that have increased survivability and yield under adverse osmotic conditions.
G2998 (SEQ ID NO: 623)
Published Information
The gene is a member of the ZF-HB family. No data have been presented publicly regarding the function of this gene.
Experimental Observations
The boundaries of G2998 were determined by RACE, and a clone was PCR-amplified from cDNA derived from mixed tissue samples. A full-length cDNA sequence has recently been deposited in GenBank (Accession AY084462), and its coding sequence is identical that identified by us. The function of G2998 was assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from a 35S CaMV promoter. All three of the 35S::G2998 T2 populations analyzed, displayed an enhanced ability to germinate on plates containing high levels of sodium chloride. Thus, G2998 can function as part of response pathway to abiotic stress. Additionally, morphological studies revealed that overexpression of G2998 can produce a delay in the onset of reproductive development, indicating that the gene can have a role in determining flowering time in Arabidopsis.
Utilities
Based on the increased salt tolerance exhibited by the 35S::G2998 lines in physiology assays, this gene might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
The delayed flowering displayed by 35S::G2998 transformants indicates that the gene might be used to manipulate the flowering time of commercial species. In particular, an extension of vegetative growth can significantly increase biomass and result in substantial yield increases.
Given the effects of G2998 overexpression, it is likely that the activity of the gene (or its orthologs) could be modified to accelerate flowering, or eliminate any requirement for vernalization.
G2999 (SEQ ID NO: 625)
Published Information
G2999 was identified within a sequence released by the Arabidopsis Genome Initiative (Chromosome 2, GenBank accession AC006439).
Experimental Observations
The boundaries of G2999 were determined by RACE experiments and a full-length clone was PCR-amplified out of cDNA derived from mixed tissues. The function of G2999 was then assessed by analysis of transgenic Arabidopsis lines in which the cDNA was constitutively expressed from a 35S CaMV promoter. 35S::G2999 transformants displayed wild-type morphology, but two of three T2 lines showed increased tolerance to salt stress in the physiology assays. Root growth assays with G2999 overexpressing seedlings and controls in a high sodium chloride medium showed that a majority of 35S::G2999 Arabidopsis seedlings appeared larger, greener, and had more root growth than the control seedlings on the right ( FIG. 8C , four control seedling are on the right). G2998, a paralogous Arabidopsis sequence, also showed a salt phenotype and performed similarly in the plate-based salt stress assay ( FIG. 8B ). Thus, G2998 and G2999 could act in the same pathways, and have a role in the response to abiotic stress.
Utilities
Given the salt resistance exhibited by 35S::G2999 transformants, the gene might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions.
G3070 (SEQ ID NO: 653)
Published Information
G3070 was identified in the sequence of BAC T23J18, GenBank accession number AC011661, released by the Arabidopsis Genome Initiative. There is no other published or public information about the function of G3070.
Experimental Observations
The 5′ end of G3070 was determined by RACE PCR. The function of G3070 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. 35S::G3070 transformants had leaves with distinctive steely gray coloration at all stages of the life cycle. In all other respects, however, the plants appeared morphologically normal. This dramatic change in leaf color might have arisen from a variety of possible causes, including a change in the level of pigments, alterations in wax accumulation/composition at the leaf surface, or by a change in the histology of the leaves. Alterations in cell shape or changes in the adhesion of epidermis to underlying cell layers have been found to result in coloration changes (Cornish and Zeevart (1986) Plant Physiol 81: 1017–1021; Glover et al. (1998) Development 125: 3497–3508; Heys et al. (1997) Planta 202: 85–92). There was no consistent difference in physiological assays between 35S::G3070 transformants and wild-type seedlings.
Utilities
Depending on the basis of the color change seen in 35S::G3070 lines, a number of applications could be envisaged.
If the phenotype is due to loosening of epidermal cell layers, the gene or its equivalogs might be used to produce fruits, vegetables, and other plant products, which can be more easily peeled.
If the effects are due to changes in wax composition and/or accumulation, G3070 or its equivalogs might be used to afford protection against pests or abiotic stresses such as drought.
If the phenotype is due to changes in pigment levels within the leaf, the gene or its equivalogs may be applied to alter photosynthetic capacity and yield.
G3076 (SEQ ID NO: 655)
Published Information
G3076 (At4g18650) was identified as part of the BAC clone F28A21 (GenBank accession AL035526).
Experimental Observations
The function of G3076 was studied using plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G3076 produced no consistent alterations in Arabidopsis growth and development. However, G3076 overexpressing lines showed more tolerance to a severe drought stress treatment.
Utilities
The reduced sensitivity of 35S::G3076 lines in the dehydration assay indicated that the gene or its equivalogs might be used to engineer crops with increased water use efficiency or increased tolerance to stresses such as drought, salt, freezing and/or chilling stress.
G3083 (SEQ ID NO: 657)
Published Information
G3083 (At3g14880) is part of BAC clone K15M2, GenBank accession number AP000370 (nid=5541653).
Experimental Observations
The 5′- and 3′-ends of G3083 were determined by RACE and the function of the gene was assessed by analysis of transgenic Arabidopsis lines in which a genomic clone was constitutively expressed from a 35S promoter. In the physiological analysis, two out of the three 35S::G3083 lines tested, displayed an enhanced ability to germinate on plates containing high levels of sodium chloride. Thus, G3083 can function as part of a response pathway to abiotic stress. 35S::G3083 plants were indistinguishable from wild-type controls in the morphological analysis.
Utilities
Based on the increased salt tolerance exhibited by the 35S::G3083 lines in physiology assays, this gene might be used to engineer salt tolerant crops and trees that can flourish in salinified soils, or under drought conditions.
G3086 (SEQ ID NO: 661)
Published Information
G3086 corresponds to gene AT1G51140, annotated by the Arabidopsis Genome Initiative. No information is available about the function(s) of G3086.
Experimental Observations
The function of G3086 was studied using transgenic plants in which the gene was expressed under the control of the 35S promoter. Overexpression of G3086 in Arabidopsis produced a pronounced acceleration in the onset of flowering. 35S::G3086 transformants produced visible flower buds 5–7 days early (in inductive 24-hour light conditions), and were markedly smaller than wild-type controls.
G3086 overexpressing lines were larger and more tolerant of heat stress. FIG. 9A shows the effects of a heat assay on Arabidopsis wild-type and G3086-overexpressing plants. The overexpressors on the left were generally larger, paler, and exhibited earlier bolting than the wild type plants seen on the right of this plate.
35S::G3086 transformants were also larger and displayed more root growth when grown under high salt conditions. G3086 overexpressors, as exemplified by the eight seedlings on the right of FIG. 9B , were larger, greener, and had more root growth than control plants, as exemplified by the four seedlings on the right in FIG. 9B .
Utilities
Based on the phenotypes observed in morphological and physiological assays, G3086 might be have a number of utilities.
Given the salt resistance exhibited by 35S::G3086 transformants, the gene or its orthologs might be used to engineer salt tolerant crops and trees that can flourish in saline soils, or under drought conditions.
Based on the response of 35S::G3086 lines to heat stress, the gene or its orthologs might be used to engineer crop plants with increased tolerance to abiotic stresses such as high temperatures, a stress that often occurs simultaneously with other environmental stress conditions such as drought or salt stress.
The early flowering displayed by 35S::G3086 transformants indicated that the gene or its orthologs might be used to accelerate the flowering of commercial species, or to eliminate any requirements for vernalization.
Example IX
Identification of Homologous Sequences
This example describes identification of genes that are orthologous to Arabidopsis thaliana transcription factors from a computer homology search.
Homologous sequences, including those of paralogs and orthologs from Arabidopsis and other plant species, were identified using database sequence search tools, such as the Basic Local Alignment Search Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403–410; and Altschul et al. (1997) Nucleic Acid Res. 25: 3389–3402). The tblastx sequence analysis programs were employed using the BLOSUM-62 scoring matrix (Henikoff and Henikoff (1992) Proc. Natl. Acad. Sci. 89: 10915–10919). The entire NCBI GenBank database was filtered for sequences from all plants except Arabidopsis thaliana by selecting all entries in the NCBI GenBank database associated with NCBI taxonomic ID 33090 (Viridiplantae; all plants) and excluding entries associated with taxonomic ID 3701 ( Arabidopsis thaliana ).
These sequences are compared to sequences representing genes of the Sequence Listing, for example, SEQ ID NO: 2N−1, wherein N=1–335, using the Washington University TBLASTX algorithm (version 2.0a19MP) at the default settings using gapped alignments with the filter “off”. For each of these gene sequences, individual comparisons were ordered by probability score (P-value), where the score reflects the probability that a particular alignment occurred by chance. For example, a score of 3.6e-40 is 3.6×10–40. In addition to P-values, comparisons were also scored by percentage identity. Percentage identity reflects the degree to which two segments of DNA or protein are identical over a particular length. Examples of sequences so identified are presented in Tables 7, 8 and 9. Paralogous or orthologous sequences were readily identified from proprietary databases and in GenBank. The percent sequence identity among these sequences can be as low as 47%, or even lower sequence identity.
Candidate paralogous sequences were identified among Arabidopsis transcription factors through alignment, identity, and phylogenic relationships. A list of paralogs is shown in Table 8. Candidate orthologous sequences were identified from proprietary unigene sets of plant gene sequences in Zea mays, Glycine max and Oryza sativa based on significant homology to Arabidopsis transcription factors. These candidates were reciprocally compared to the set of Arabidopsis transcription factors. If the candidate showed maximal similarity in the protein domain to the eliciting transcription factor or to a paralog of the eliciting transcription factor, then it was considered to be an ortholog. Identified non- Arabidopsis sequences that were shown in this manner to be orthologous to the Arabidopsis sequences are provided in Tables 7 and 9.
Example X
Screen of Plant cDNA library for Sequence Encoding a Transcription Factor DNA Binding Domain that Binds to a Transcription Factor Binding Promoter Element and Demonstration of Protein Transcription Regulation Activity
The “one-hybrid” strategy (Li and Herskowitz (1993) Science 262: 1870–1874) is used to screen for plant cDNA clones encoding a polypeptide comprising a transcription factor DNA binding domain, a conserved domain. In brief, yeast strains are constructed that contain a lacZ reporter gene with either wild-type or mutant transcription factor binding promoter element sequences in place of the normal UAS (upstream activator sequence) of the GAL4 promoter. Yeast reporter strains are constructed that carry transcription factor binding promoter element sequences as UAS elements are operably linked upstream (5′) of a lacZ reporter gene with a minimal GAL4 promoter. The strains are transformed with a plant expression library that contains random cDNA inserts fused to the GAL4 activation domain (GAL4-ACT) and screened for blue colony formation on X-gal-treated filters (X-gal: 5-bromo-4-chloro-3-indolyl-β-D-galactoside; Invitrogen Corporation, Carlsbad Calif.). Alternatively, the strains are transformed with a cDNA polynucleotide encoding a known transcription factor DNA binding domain polypeptide sequence.
Yeast strains carrying these reporter constructs produce low levels of beta-galactosidase and form white colonies on filters containing X-gal. The reporter strains carrying wild-type transcription factor binding promoter element sequences are transformed with a polynucleotide that encodes a polypeptide comprising a plant transcription factor DNA binding domain operably linked to the acidic activator domain of the yeast GAL4 transcription factor, “GAL4-ACT”. The clones that contain a polynucleotide encoding a transcription factor DNA binding domain operably linked to GAL4-ACT can bind upstream of the lacZ reporter genes carrying the wild-type transcription factor binding promoter element sequence, activate transcription of the lacZ gene and result in yeast forming blue colonies on X-gal-treated filters.
Upon screening about 2×10 6 yeast transformants, positive cDNA clones are isolated; i.e., clones that cause yeast strains carrying lacZ reporters operably linked to wild-type transcription factor binding promoter elements to form blue colonies on X-gal-treated filters. The cDNA clones do not cause a yeast strain carrying a mutant type transcription factor binding promoter elements fused to LacZ to turn blue. Thus, a polynucleotide encoding transcription factor DNA binding domain, a conserved domain, is shown to activate transcription of a gene.
Example XI
Gel Shift Assays
The presence of a transcription factor comprising a DNA binding domain which binds to a DNA transcription factor binding element is evaluated using the following gel shift assay. The transcription factor is recombinantly expressed and isolated from E. coli or isolated from plant material. Total soluble protein, including transcription factor, (40 ng) is incubated at room temperature in 10 μl of 1× binding buffer (15 mM HEPES (pH 7.9), 1 mM EDTA, 30 mM KCl, 5% glycerol, 5% bovine serum albumin, 1 mM DTT) plus 50 ng poly(dl-dC):poly(dl-dC) (Pharmacia, Piscataway N.J.) with or without 100 ng competitor DNA. After 10 minutes incubation, probe DNA comprising a DNA transcription factor binding element (1 ng) that has been 32 P-labeled by end-filling (Sambrook et al. (1989) supra) is added and the mixture incubated for an additional 10 minutes. Samples are loaded onto polyacrylamide gels (4% w/v) and fractionated by electrophoresis at 150V for 2 h (Sambrook et al. supra). The degree of transcription factor-probe DNA binding is visualized using autoradiography. Probes and competitor DNAs are prepared from oligonucleotide inserts ligated into the BamHI site of pUC118 (Vieira et al. (1987) Methods Enzymol. 153: 3–11). Orientation and concatenation number of the inserts are determined by dideoxy DNA sequence analysis (Sambrook et al. supra). Inserts are recovered after restriction digestion with EcoRI and HindIII and fractionation on polyacrylamide gels (12% w/v) (Sambrook et al. supra).
Example XII
Introduction of Polynucleotides into Dicotyledonous Plants
Any of the transcription factor sequences of the invention listed in the Sequence Listing, and paralogous, and orthologous sequences, may be recombined into pMEN20 or pMEN65 expression vectors and then are transformed into a plant for the purpose of modifying plant traits. The cloning vector may be introduced into a variety of cereal plants by means well known in the art such as, for example, direct DNA transfer or Agrobacterium tumefaciens -mediated transformation. It is now routine to produce transgenic plants using most dicot plants (see Weissbach and Weissbach, (1989) supra; Gelvin et al. (1990) supra; Herrera-Estrella et al. (1983) supra; Bevan (1984) supra; and Klee (1985) supra). Methods for analysis of traits are routine in the art and examples are disclosed above.
Example XIII
Transformation of Cereal Plants with an Expression Vector
Cereal plants such as, but not limited to, corn, wheat, rice, sorghum, or barley, may also be transformed with the present polynucleotide sequences in pMEN20 or pMEN65 expression vectors for the purpose of modifying plant traits. For example, pMEN020 may be modified to replace the NptII coding region with the BAR gene of Streptomyces hygroscopicus that confers resistance to phosphinothricin. The KpnI and BglII sites of the Bar gene are removed by site-directed mutagenesis with silent codon changes.
The cloning vector may be introduced into a variety of cereal plants by means well known in the art such as, for example, direct DNA transfer or Agrobacterium tumefaciens -mediated transformation. It is now routine to produce transgenic plants of most cereal crops (Vasil (1994) Plant Mol. Biol. 25: 925–937) such as corn, wheat, rice, sorghum (Cassas et al. (1993) Proc. Natl. Acad. Sci. 90: 11212–11216, and barley (Wan and Lemeaux (1994) Plant Physiol. 104:37–48. DNA transfer methods such as the microprojectile can be used for corn (Fromm et al. (1990) Bio/Technol. 8: 833–839); Gordon-Kamm et al. (1990) Plant Cell 2: 603–618; Ishida (1990) Nature Biotechnol. 14:745–750), wheat (Vasil et al. (1992) Bio/Technol. 10:667–674; Vasil et al. (1993) Bio/Technol. 11:1553–1558; Weeks et al. (1993) Plant Physiol. 102:1077–1084), rice (Christou (1991) Bio/Technol. 9:957–962; Hiei et al. (1994) Plant J. 6:271–282; Aldemita and Hodges (1996) Planta 199:612–617; and Hiei et al. (1997) Plant Mol. Biol. 35:205–218). For most cereal plants, embryogenic cells derived from immature scutellum tissues are the preferred cellular targets for transformation (Hiei et al. (1997) Plant Mol. Biol. 35:205–218; Vasil (1994) Plant Mol. Biol. 25: 925–937).
Vectors according to the present invention may be transformed into corn embryogenic cells derived from immature scutellar tissue by using microprojectile bombardment, with the A188XB73 genotype as the preferred genotype (Fromm et al. (1990) Bio/Technol. 8: 833–839; Gordon-Kamm et al. (1990) Plant Cell 2: 603–618). After microprojectile bombardment the tissues are selected on phosphinothricin to identify the transgenic embryogenic cells (Gordon-Kamm et al. (1990) Plant Cell 2: 603–618). Transgenic plants are regenerated by standard corn regeneration techniques (Fromm et al. (1990) Bio/Technol. 8: 833–839; Gordon-Kamm et al. (1990) Plant Cell 2: 603–618).
The plasmids prepared as described above can also be used to produce transgenic wheat and rice plants (Christou (1991) Bio/Technol. 9:957–962; Hiei et al. (1994) Plant J. 6:271–282; Aldemita and Hodges (1996) Planta 199:612–617; and Hiei et al. (1997) Plant Mol. Biol. 35:205–218) that coordinately express genes of interest by following standard transformation protocols known to those skilled in the art for rice and wheat (Vasil et al. (1992) Bio/Technol. 10:667–674; Vasil et al. (1993) Bio/Technol. 11:1553–1558; and Weeks et al. (1993) Plant Physiol. 102:1077–1084), where the bar gene is used as the selectable marker.
Example XIV
Identification of Orthologous and Paralogous Sequences
Orthologs to Arabidopsis genes may identified by several methods, including hybridization, amplification, or bioinformatically. This example describes how one may identify homologs to the Arabidopsis AP2 family transcription factor CBF1 (polynucleotide SEQ ID NO: 2238, encoded polypeptide SEQ ID NO: 2239), which confers tolerance to abiotic stresses (Thomashow et al. (2002) U.S. Pat. No. 6,417,428), and an example to confirm the function of homologous sequences. In this example, orthologs to CBF1 were found in canola ( Brassica napus ) using polymerase chain reaction (PCR).
Degenerate primers were designed for regions of AP2 binding domain and outside of the AP2 (carboxyl terminal domain):








Mol 368
5′- CAY CCN ATH TAY MGN GGN GT -3′


(reverse)
(SEQ ID NO: 2246)





Mol 378
5′- GGN ARN ARC ATN CCY TCN GCC -3′


(forward)
(SEQ ID NO: 2247)








(Y: C/T, N: A/C/G/T, H: A/C/T, M: A/C, R: A/G)

















Primer Mol 368 is in the AP2 binding domain of CBF1 (amino acid sequence: His-Pro-Ile-Tyr-Arg-Gly-Val) while primer Mol 378 is outside the AP2 domain (carboxyl terminal domain) (amino acid sequence: Met-Ala-Glu-Gly-Met-Leu-Leu-Pro).
The genomic DNA isolated from B. napus was PCR-amplified by using these primers following these conditions: an initial denaturation step of 2 min at 93° C.; 35 cycles of 93° C. for 1 min, 55° C. for 1 min, and 72° C. for 1 min; and a final incubation of 7 min at 72° C. at the end of cycling.
The PCR products were separated by electrophoresis on a 1.2% agarose gel and transferred to nylon membrane and hybridized with the AT CBF1 probe prepared from Arabidopsis genomic DNA by PCR amplification. The hybridized products were visualized by colorimetric detection system (Boehringer Mannheim) and the corresponding bands from a similar agarose gel were isolated using the Qiagen Extraction Kit (Qiagen). The DNA fragments were ligated into the TA clone vector from TOPO TA Cloning Kit (Invitrogen) and transformed into E. coli strain TOP10 (Invitrogen).
Seven colonies were picked and the inserts were sequenced on an ABI 377 machine from both strands of sense and antisense after plasmid DNA isolation. The DNA sequence was edited by sequencer and aligned with the AtCBF1 by GCG software and NCBI blast searching.
The nucleic acid sequence and amino acid sequence of one canola ortholog found in this manner (bnCBF1; polynucleotide SEQ ID NO: 2244 and polypeptide SEQ ID NO: 2245) identified by this process is shown in the Sequence Listing.
The aligned amino acid sequences show that the bnCBF1 gene has 88% identity with the Arabidopsis sequence in the AP2 domain region and 85% identity with the Arabidopsis sequence outside the AP2 domain when aligned for two insertion sequences that are outside the AP2 domain.
Similarly, paralogous sequences to Arabidopsis genes, such as CBF1, may also be identified.
Two paralogs of CBF1 from Arabidopsis thaliana : CBF2 and CBF3. CBF2 and CBF3 have been cloned and sequenced as described below. The sequences of the DNA SEQ ID NO: 2240 and 2242 and encoded proteins SEQ ID NO: 2241 and 2243 are set forth in the Sequence Listing.
A lambda cDNA library prepared from RNA isolated from Arabidopsis thaliana ecotype Columbia (Lin and Thomashow (1992) Plant Physiol. 99: 519–525) was screened for recombinant clones that carried inserts related to the CBF1 gene (Stockinger et al. (1997) Proc. Natl. Acad. Sci. 94:1035–1040). CBF1 was 32 P-radiolabeled by random priming (Sambrook et al. supra) and used to screen the library by the plaque-lift technique using standard stringent hybridization and wash conditions (Hajela et al. (1990) Plant Physiol. 93:1246–1252; Sambrook et al. supra) 6× SSPE buffer, 60° C. for hybridization and 0.1× SSPE buffer and 60° C. for washes). Twelve positively hybridizing clones were obtained and the DNA sequences of the cDNA inserts were determined. The results indicated that the clones fell into three classes. One class carried inserts corresponding to CBF1. The two other classes carried sequences corresponding to two different homologs of CBF1, designated CBF2 and CBF3. The nucleic acid sequences and predicted protein coding sequences for Arabidopsis CBF1, CBF2 and CBF3 are listed in the Sequence Listing (SEQ ID NOs:2238, 2240, 2242 and SEQ ID NOs: 2239, 2241, and 2243, respectively). The nucleic acid sequences and predicted protein coding sequence for Brassica napus CBF ortholog is listed in the Sequence Listing (SEQ ID NOs: 2244 and 2245, respectively).
A comparison of the nucleic acid sequences of Arabidopsis CBF1, CBF2 and CBF3 indicate that they are 83 to 85% identical as shown in Table 11.








TABLE 11





Percent identity a







DNA b
Polypeptide










cbf1/cbf2
85
86


cbf1/cbf3
83
84


cbf2/cbf3
84
85





a Percent identity was determined using the Clustal algorithm from the Megalign program (DNASTAR, Inc.).


b Comparisons of the nucleic acid sequences of the open reading frames are shown.


























Similarly, the amino acid sequences of the three CBF polypeptides range from 84 to 86% identity. An alignment of the three amino acid sequences reveals that most of the differences in amino acid sequence occur in the acidic C-terminal half of the polypeptide. This region of CBF1 serves as an activation domain in both yeast and Arabidopsis (not shown).
Residues 47 to 106 of CBF1 correspond to the AP2 domain of the protein, a DNA binding motif that to date, has only been found in plant proteins. A comparison of the AP2 domains of CBF1, CBF2 and CBF3 indicates that there are a few differences in amino acid sequence. These differences in amino acid sequence might have an effect on DNA binding specificity.
Example XV
Transformation of Canola with a Plasmid Containing CBF1, CBF2, or CBF3
After identifying homologous genes to CBF1, canola was transformed with a plasmid containing the Arabidopsis CBF1, CBF2, or CBF3 genes cloned into the vector pGA643 (An (1987) Methods Enzymol. 253: 292). In these constructs the CBF genes were expressed constitutively under the CaMV 35S promoter. In addition, the CBF1 gene was cloned under the control of the Arabidopsis COR15 promoter in the same vector pGA643. Each construct was transformed into Agrobacterium strain GV3101. Transformed Agrobacteria were grown for 2 days in minimal AB medium containing appropriate antibiotics.
Spring canola ( B. napus cv. Westar) was transformed using the protocol of Moloney et al. ((1989) Plant Cell Reports 8: 238) with some modifications as described. Briefly, seeds were sterilized and plated on half strength MS medium, containing 1% sucrose. Plates were incubated at 24° C. under 60–80 μE/m 2 s light using a 16 hour light/8 hour dark photoperiod. Cotyledons from 4–5 day old seedlings were collected, the petioles cut and dipped into the Agrobacterium solution. The dipped cotyledons were placed on co-cultivation medium at a density of 20 cotyledons/plate and incubated as described above for 3 days. Explants were transferred to the same media, but containing 300 mg/l timentin (SmithKline Beecham, Pa.) and thinned to 10 cotyledons/plate. After 7 days explants were transferred to Selection/Regeneration medium. Transfers were continued every 2–3 weeks (2 or 3 times) until shoots had developed. Shoots were transferred to Shoot-Elongation medium every 2–3 weeks. Healthy looking shoots were transferred to rooting medium. Once good roots had developed, the plants were placed into moist potting soil.
The transformed plants were then analyzed for the presence of the NPTII gene/kanamycin resistance by ELISA, using the ELISA NPTII kit from 5Prime-3Prime Inc. (Boulder, Colo.). Approximately 70% of the screened plants were NPTII positive. Only those plants were further analyzed.
From Northern blot analysis of the plants that were transformed with the constitutively expressing constructs, showed expression of the CBF genes and all CBF genes were capable of inducing the Brassica napus cold-regulated gene BN115 (homolog of the Arabidopsis COR15 gene). Most of the transgenic plants appear to exhibit a normal growth phenotype. As expected, the transgenic plants are more freezing tolerant than the wild-type plants. Using the electrolyte leakage of leaves test, the control showed a 50% leakage at −2 to −3° C. Spring canola transformed with either CBF1 or CBF2 showed a 50% leakage at −6 to −7° C. Spring canola transformed with CBF3 shows a 50% leakage at about −10 to −15° C. Winter canola transformed with CBF3 may show a 50% leakage at about −16 to −20° C. Furthermore, if the spring or winter canola are cold acclimated the transformed plants may exhibit a further increase in freezing tolerance of at least −2° C.
To test salinity tolerance of the transformed plants, plants were watered with 150 mM NaCl. Plants overexpressing CBF1, CBF2 or CBF3 grew better compared with plants that had not been transformed with CBF1, CBF2 or CBF3.
These results demonstrate that homologs of Arabidopsis transcription factors can be identified and shown to confer similar functions in non-Arabidopsis plant species.
Example XVI
Cloning of Transcription Factor Promoters
Promoters are isolated from transcription factor genes that have gene expression patterns useful for a range of applications, as determined by methods well known in the art (including transcript profile analysis with cDNA or oligonucleotide microarrays, Northern blot analysis, semi-quantitative or quantitative RT-PCR). Interesting gene expression profiles are revealed by determining transcript abundance for a selected transcription factor gene after exposure of plants to a range of different experimental conditions, and in a range of different tissue or organ types, or developmental stages. Experimental conditions to which plants are exposed for this purpose includes cold, heat, drought, osmotic challenge, varied hormone concentrations (ABA, GA, auxin, cytokinin, salicylic acid, brassinosteroid), pathogen and pest challenge. The tissue types and developmental stages include stem, root, flower, rosette leaves, cauline leaves, siliques, germinating seed, and meristematic tissue. The set of expression levels provides a pattern that is determined by the regulatory elements of the gene promoter.
Transcription factor promoters for the genes disclosed herein are obtained by cloning 1.5 kb to 2.0 kb of genomic sequence immediately upstream of the translation start codon for the coding sequence of the encoded transcription factor protein. This region includes the 5′-UTR of the transcription factor gene, which can comprise regulatory elements. The 1.5 kb to 2.0 kb region is cloned through PCR methods, using primers that include one in the 3′ direction located at the translation start codon (including appropriate adaptor sequence), and one in the 5′ direction located from 1.5 kb to 2.0 kb upstream of the translation start codon (including appropriate adaptor sequence). The desired fragments are PCR-amplified from Arabidopsis Col-0 genomic DNA using high-fidelity Taq DNA polymerase to minimize the incorporation of point mutation(s). The cloning primers incorporate two rare restriction sites, such as Not1 and Sfi1, found at low frequency throughout the Arabidopsis genome. Additional restriction sites are used in the instances where a Not1 or Sfi1 restriction site is present within the promoter.
The 1.5–2.0 kb fragment upstream from the translation start codon, including the 5′-untranslated region of the transcription factor, is cloned in a binary transformation vector immediately upstream of a suitable reporter gene, or a transactivator gene that is capable of programming expression of a reporter gene in a second gene construct. Reporter genes used include green fluorescent protein (and related fluorescent protein color variants), beta-glucuronidase, and luciferase. Suitable transactivator genes include LexA-GAL4, along with a transactivatable reporter in a second binary plasmid (as disclosed in U.S. patent application Ser. No. 09/958,131, incorporated herein by reference). The binary plasmid(s) is transferred into Agrobacterium and the structure of the plasmid confirmed by PCR. These strains are introduced into Arabidopsis plants as described in other examples, and gene expression patterns determined according to standard methods know to one skilled in the art for monitoring GFP fluorescence, beta-glucuronidase activity, or luminescence.
The promoter region for G1753 is obtained from Arabidopsis chromosome 2 clone F1011 (AC006919), gene At2g36450, from position 43906–45410 of the genomic clone. The complement of this sequence is the promoter oriented in the 5′-3′ direction, with the translation start codon for G1753 the complement of positions 43903–43905.
The present invention is not limited by the specific embodiments described herein. The invention now being fully described, it will be apparent to one of ordinary skill in the art that many changes and modifications can be made thereto without departing from the spirit or scope of the appended claims. Modifications that become apparent from the foregoing description and accompanying figures fall within the scope of the claims.
All references, publications, patent documents, web pages, and other documents cited or mentioned herein are hereby incorporated by reference in their entirety for all purposes. Although the invention has been described with reference to specific embodiments and examples, it should be understood that one of ordinary skill can make various modifications without departing from the spirit of the invention. The scope of the invention is not limited to the specific embodiments and examples provided.