(en)An improved stereoselective process for the preparation of 6α-fluoropregnane derivatives of formula (I), comprising the reaction of 3-benzoyloxy Δ3,5-pregnane derivatives of formula (II), with an electrophilic fluorination agent in a substantially water-free reaction mixture and in the presence of a salt of a strong acid with a nitrogenous base.

1.ApplicationNumber: US-53791603-A
1.PublishNumber: US-2006252926-A1
2.Date Publish: 20061109
3.Inventor: MACDONALD PETER
ROSSETTO PIERLUIGI

4.Inventor Harmonized: MACDONALD PETER(CH)
ROSSETTO PIERLUIGI(CH)

5.Country: US
6.Claims:
(en)An improved stereoselective process for the preparation of 6α-fluoropregnane derivatives of formula (I), comprising the reaction of 3-benzoyloxy Δ3,5-pregnane derivatives of formula (II), with an electrophilic fluorination agent in a substantially water-free reaction mixture and in the presence of a salt of a strong acid with a nitrogenous base.

7.Description:
(en)The present invention refers to an improved stereoselective process for the preparation of 6α-fluoropregnanes, comprising the fluorination with an electrophilic fluorination agent in the 6-position of selected pregnane derivatives in the presence of an amine salt of a strong acid under substantially water-free reaction conditions.
Fluorstereoids represent useful antiinflammatory compounds and it is known that stereoisomers have different pharmacological efficiencies. It is also known that 6α-fluoropregnane derivatives have in general a higher efficiency than corresponding 6β-fluoro analogues. Several processes have been developed for obtaining 6α-fluoropregnanes, but all these processes suffer from poor stereoselectivity. There is a need for a process giving a higher ratio of 6α- to 6β-stereoisomers for a more economic industrial-scale manufacture.
F. La Loggia et al. describe in US Patent Application Publication 2002/0062021 a process for the fluorination of pregnanes having the formula


wherein R is chosen from H, OH, and an alkyl group with from 1 to 4 carbon atoms and R′ is an alkyl group with from 1 to 4 carbon atoms, using electrophilic fluorination agents. Own investigations have shown that this process leads to reaction mixtures containing 6α/6βratios of not higher than 90:10. Partial elimination of 6β-isomers could sometimes be obtained, but with an accompanying loss of product, by filtration of undissolved products from the reaction mixture. Pure 6α-fluoro derivatives can therefore only be obtained after further purification and/or isomerisation steps (see U.S. Pat. No. 6,369,218), both of which inevitably lead to additional costs and significant losses of product.

J.-Y. Godard et al. disclose in U.S. Pat. No. 5,478,957 a process for the introduction of a 6α-fluoro function into an androstane enol benzoate derivative (3-benzoyloxy-9β,11β-epoxy-16α-methyl-17β-methoxycarbonyl-Δ1,3,5-androstatrien-17α-ol) using an electrophilic fluorination agent, preferably Selectfluor®, in a water-containing solvent. We have found that the crude product (6α-fluoro-9β,11β-epoxy-16α-methyl-17β-methoxycarbonyl-Δ1,4-androstadien-17α-ol-3-one) obtained in Stage D contains at least 4% of 6β-isomer, as well as significant amounts of 6-hydroxylated by-product. Application of the reaction conditions described in U.S. Pat. No. 5,478,957 to an enol benzoate of a pregnane gave even more (6.5%) 6β-isomer formation, as well as a significant amount of 6-hydroxylated by-product. The purity of 6α-fluoropregnanes that could be obtained using the conditions of U.S. Pat. No. 5,478,957 is thus unsatisfactory, and further purification would be necessary in order to obtain products suitable for pharmaceutical formulations. Even when such purifications are practicable, considerable amounts of the desired 6α-isomer are inevitably lost during the purification steps. It is also to be noted that, although the conversion of a pregnane into its corresponding androstane derivative through side-chain removal is relatively efficient (see, for example, Stage A of Example 1 in U.S. Pat. No. 5,478,957), the reverse (reconstruction of the pregnane side-chain) constitutes a difficult multi-step operation. The products of U.S. Pat. No. 5,478,957 are therefore not at all useful for obtaining valuable 6α-fluorinated pregnanes such as diflorasone, flumethasone, difluprednate, fluocinolone acetonide, fluocinonide, flunisolide, and others.
WO 02/100878 A1 describes a process for the preparation of flumethasone, where 9β,11-epoxy-16α-methyl-3,17,21-trihydroxy-pregna-1,3,5-triene-20-one-21-acetate-3-benzoate is used as an intermediate for fluorination in 6-position with an electrophilic fluorination agent.
It has surprisingly now been found that the stereoselectivity of electrophilic 6α-fluorinations of pregnanes can be greatly enhanced, and that the formation of side-products can be substantially suppressed, if 3-benzoyloxy-Δ3,5-pregnane derivatives are selected as substrates and the fluorination is carried out in a substantially water-free reaction mixture in the presence of a salt of a strong acid with a nitrogenous base. Under these conditions 6α/6β ratios of up to 99:1 can be obtained in reaction mixtures, with negligible formation of 6-hydroxylated, 4-fluorinated, or other by-products. Moreover, according to the fluorination process of the invention, higher chemical yields are obtained and, due to the high purity of the crude products, fewer purification operations are necessary in order to obtain useful pharmaceutical products.
One object of the invention is a process for the preparation of 6α-fluoro compounds of formula I,


wherein

R 2 is hydrogen, C 1 -C 8 alkyl or C 3 -C 8 cycloalkyl; and
R 3 is hydrogen, C 1 -C 8 alkyl, or R 4 —C(O)—O— where R 4 is C 1 -C 8 alkyl or C 1 -C 8 hydroxyalkyl;
comprising the fluorination of pregnane derivatives in the 6-position with an electrophilic fluorination agent, in an inert solvent and at ambient temperatures, characterised in that (1) a compound of formula II


wherein

R 1 is phenyl or phenyl substituted with halogen, hydroxy, amino, mono- or di-C 1 -C 8 alkylamino, C 1 -C 8 alkyl, C 1 -C 8 alkoxy and/or C 1 -C 8 carbalkoxy; and R 2 and R 3 have the meanings given before;
is reacted with an electrophilic fluorination agent (2) in the presence of a salt of a strong acid with a nitrogenous base under (3) substantial water-free reaction conditions.
R 2 in formula I means preferably C 1 -C 4 alkyl and may be methyl, ethyl, n- or i-propyl, and the isomers of butyl, pentyl, hexyl, heptyl and octyl. R 2 in formula I as C 3 -C 8 cycloalkyl is preferably C 3 -C 6 cycloalkyl, and more preferably C 4 -C 6 cycloalkyl. Cycloalkyl can be for example cyclopropyl, cyclobutyl, cyclopentyl, cydohexyl, cycloheptyl and cyclooctyl. Preferred cycloalkyls are cyclopentyl and cyclohexyl.
R 2 in formula I is most preferably C 1 -C 8 alkyl and particularly preferred C 1 -C 4 alkyl.
R 3 is as alkyl preferably C 1 -C 6 alkyl, more preferably C 1 -C 4 alkyl, and mostly preferred C 1 -C 2 alkyl. Examples for alkyl are methyl, ethyl, n- or i-propyl, and the isomers of butyl, pentyl, hexyl, heptyl and octyl. R 3 is as alkyl especially preferred methyl or ethyl.
R 4 in the residue R 4 —C(O)—O— may have as alkyl the same preferred meanings as given before for R 3 . R 4 is as alkyl mostly preferred methyl or ethyl. R 4 as hydroxyalkyl may contain 1 to 4 and more preferably 1 or 2 carbon atoms. Examples are hydroxymethyl and hydroxyethyl.
In a preferred embodiment, R 3 is selected from the group of hydrogen, methyl and acetyloxy.
Inert solvents for this reaction are well known and may be selected from the group of polar and aprotic solvents. Examples are nitriles (acetonitril), N-dialkylated carboxylic acid amides (dimethyl formamide, diethyl formamide) or N-alkylated cyclic carboxylic acid amides (N-methyl pyrrolidone, N-ethyl pyrrolidone), ethers (tetrahydrofurane, dioxane) and carboxylic esters (ethylacetate, methylbenzoate).
Ambient temperatures may mean a temperature range from −20 to 50° C., preferably −10 to 40° C., and most preferably 0 to 30° C.
Preferred substituents for R 1 as phenyl are fluorine, chlorine, hydroxy, dimethylamino, methyl, ethyl, methoxy, ethoxy and methoxycarbonyl. Examples for substituted phenyl are 4-flurophenyl, 2,4-difluorophenyl, 2,4,6-trifluorophenyl, 4-chlorophenyl, 2,4-dichlorophenyl, 2- or 4-hydroxyphenyl, 4-methylphenyl, 4-ethylphenyl, 2,4-dimethylphenyl, 2,4-diethylphenyl, 2,4,6-trimethylphenyl, 4-methoxyphenyl, 4-ethoxyphenyl, 2,4-dimethoxyphenyl, 2,4-diethoxyphenyl, 2,4,6-trimethoxyphenyl, 2-methyl-4-fluorophenyl, 2-methyl-4chlorophenyl, 2- or 3- or 4-methoxycarbonylphenyl.
In a preferred embodiment R 1 is phenyl.
Suitable electrophilic fluorination agents are well known in the art and, in part, commercially available. These compounds include can for example N-fluorosulfonamides, N-fluoropyridinium salts, N-fluorobis(trifluoromethanesulfonyl)imides, N-alkyl-N′-fluoro-1,4-diazoniabicyclo[2.2.2]octane salts, N-fluoro-N′-hydroxy-1,4-diazoniabicyclo[2.2.2]octane salts, and perchloryl fluoride.
Fluorinating agents are preferably selected from N-F quarternary salts due to their commercial availability and improved safety with respect to older reagants such as perchloryl fluoride. Examples of preferred fluorination agents are 1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2,2,2]octane-bistetrafluoroborate (Selectfluor®) and 1-fluoro-4-hydroxy,1,4-diazoniabicyclo[2,2,2]octane-bistetrafluoroborate (Accufluor®).
The electrophilic fluorination agent may be used in excess without detriment, but since the excess reagent must be then destroyed after the reaction it is very desirable to avoid this additional process step. According to the process of the invention, substantially equimolar amounts of the fluorination agent and compounds of formula II are used and lead to completeness of the fluorination. Substantially equimolar amounts are therefore especially preferred. The molar ratio of compounds of formula II to fluorinating agent is preferably 1:1 to 1:0.95 and especially preferred is a molar ratio of 1:1.
Amine salts with an anion of a strong acid may correspond to formula III,

HB+A− (III),

wherein HB+ is the cation of an aliphatic, aromatic, cyclic aliphatic or cyclic aromatic nitrogenous base, and A− is the anion of a strong organic or inorganic acid.

The cation HB+ may have more than one amine group, for example 1 to 4 or 1 to 2 amine groups and anions A− are present in a number according to their positive charges.
The cation can be derived from ammonia, primary, secondary or tertiary amines, whereby tertiary amines are preferred. The amine contains preferably in total 1 to 24, more preferably 1 to 16 and mostly preferred 1 to 8 carbon atoms. The amines may contain 1 to 4, and more preferably one or two, nitrogen atoms.
The N-atoms of the amine may be substituted with C 1 -C 12 alkyl, preferably C 1 -C 6 alkyl and most preferably C 1 -C 4 alkyl, C 3 -C 8 cycloalkyl, preferably C 5 -C 6 cycloalkyl, C 4 -C 10 heterocycloalkyl, preferably C 4 -C 8 heterocycloalkyl, C 6 -C 10 aryl, C 7 -C 10 aralkyl, C 5 -C 10 heteroaryl or C 5 -C 10 heteroaralkyl. The N-atom of the amines can be part of an aliphatic or aromatic mono-cyclic or polycyclic aliphatic or aromatic ring (cyclic amines) and said N-atoms can be substituted with one residue as mentioned above. Alkyl groups at the N-atoms may be substituted, for example with C 3 -C 8 cycloalkyl, C 4 -C 10 heterocycloalkyl, C 1 -C 6 alkyl, C 1 -C 6 alkoxy or hydroxyl. cycloalkyl, heterocycloalkyl, aryl, heteroaryl, aralkyl and heteroaralkyl as well as rings of cyclic amines can be substituted for example with C 1 -C 6 alkyl, C 1 -C 6 alkoxy or hydroxyl.
Examples for amines, from which the cation HB+ is derived, are mono-, di- and preferably trialkylamines like methyl-, ethyl-, propyl-, butyl, -octyl-, dimethyl-, diethyl-, dipropyl-, dibutyl-, methyl-ethyl-, methyl-propyl-, methyl-butyl-, trimethyl-, triethyl-, tripropyl-, tributyl-, methyl-diethyl-, dimethyl-ethyl-amine. Other examples for amines, from which the cation A+ is derived, are cycloalkyl-, heterocycloalkyl-, aryl-, aralkyl-, heteroaryl and heteroaralkyl-amines, preferably tertiary amines, like cyclohexyl-, cyclohexyl-methyl-, cyclohexyl-dimethyl-, tetrahydrofuranyl-, tetrahydrofuranyl-methyl, tetrahydrofuranyl-dimethyl-, phenyl-, phenyl-methyl-, phenyl-ethyl-, phenyl-butyl-, phenyl-dimethyl-, phenyl-diethyl-, benzyl-, benzyl-methyl-, benzyl-ethyl-, benzyl-isopropyl-, benzyl-butyl-, benzyl-dimethyl-, benzyl-diethyl-, furanyl-, furanyl-methyl-, furanyl-dimethyl-, thiophenyl-, thiophenyl-methyl-, thiophenyl-dimethyl-, furanylmethyl-, furanylmethyl-dimethyl-amine. Examples for amines with substituted residues are ethanolamine, diethanolamine, triethanolamine, and N,N-dimethyl-ethanolamine.
Examples for aliphatic, cyclic or aromatic amines and for polyamines having more than one amino group are pyrrolidine, piperidine, N-methyl-pyrrolidine, N-methyl-piperidine, N-methyl-morpholine, dimethylamino-N-methyl-piperidine, N,N′-diazabicycloheptane-, N,N′-diazabicyclononane, 2H-pyrrole, imidazole, pyrazole, pyridine, pyrazine, pyrimidine, pyridazine, 3H-indole, 1H-indazole, purine isoquinoline, quinoline, phthalazine, naphthydrine, quinoxaline, quinazoline, pteridine, acridine, phenanthroline, phenazine, imidazoline, triazine, 2-piccoline, lutidine, benzimidazole, methylimidazole, pyrazole, 4-dimethylaminopyridine, 4-pyrrolidinopyridine, 1,3,5-triazine and 4-methylaminopyridine.
Preferred amines are selected from the group of cyclic, polycyclic and aromatic amines and aromatic amines are especially preferred. Preferred aromatic amines are pyridine and pyrimidine.
The anion may be derived from inorganic or organic acids and the anion is preferably selected from acids which do not cause side reactions like halogenations to avoid contamination of the desired product with impurities. Examples for anions of inorganic acids are halides, hydrogen sulphate, sulphate, mono- or di-hydrogen phosphate, and phosphate. Examples for anions of organic acids are carboxylates, sulfonates, phosphinates and phosphonates. The organic residues of the organic anions may be substituted, for example with halogen and especially fluorine, hydroxy, C 1 -C 8 alkyl or C 1 -C 8 alkoxy. Specific examples are formiate, acetate, trifluoracetate, oxalate, malonate, benzoate, fluorinated benzoates, methylsulfonate, trifluormethylsulfonate, phenylsulfonate, p-toluylsulfonate, fluorphenylsulfonate, methylphosphonate, phenylphosphonate. Especially preferred are sulfonates like methylsulfonates.
A particularly preferred amine salt is pyridine methylsulfonate.
The amount of amine salts can vary in a wide range and may be from 0.1 to 100 percent by weight, preferably 1 to 100 percent by weight, more preferably 5 to 100 percent by weight and mostly preferred 10 to 100 percent by weight, referred to the amount of compounds of formula II. It was found that a range of 50 to less than 100 percent by weight, for example 50 to 90 percent by weight, is useful in carrying out the process according to the invention.
The amine salts of formula III can be added to the reaction per se as pre-formed salts or the amine salts can be formed in situ in adding an amine and a strong acid to the reaction mixture, which is a preferred embodiment for carrying out the process according to the invention.
Substantial water-free reaction conditions in the context of the invention means that no water is added to the reaction mixture. Solvents and chemicals must not be dried and the presence of traces of water does not affect the reaction.
Another object of the invention are compounds of formula II as valuable intermediates for the process according to the invention,


wherein R 1 , R 2 and R 3 have the meanings given before, with the proviso that R 1 is not phenyl, when R 2 and R 3 both are methyl. R 1 , R 2 and R 3 includes preferred embodiments given before.

Compounds of formula II are obtained in known manner and known or analogous processes through esterification or transesterification of 9,11β-epoxy-Δ4-pregnane-17β-ol-21-hydroxy-3,20-diones with carboxylic acids or derivatives thereof like carboxylic acid halides, anhydrides or esters. The preparation is advantageously carried out in two steps, since usually two different carboxylic acid residues must be introduced. In a first reaction step, the 21-hydroxy group can be selectively esterified for example with carboxylic add anhydrides like acetic anhydride. In a second reaction step is formed the 3-enol ester with benzene carboxylic acid halides (bromides or chlorides). More details regarding this reaction are given in the examples. The compounds of formula II are obtained in high yields and purity and the crude reaction product can directly be used after separation from the reaction mixture for the fluorination according to the process of the instant invention. The crude reaction product can contain benzoic acid alkyl esters, which are formed during preparation through the addition of alkanols, for example methanol, ethanol, propanol or butanol. The amounts may be up to 30 percent by weight and preferably 0.1 to 20 percent by weight, referred to compounds of formula II. It may be advantageous to add said benzoic add alkyl esters to the reaction mixture, when isolated and purified compounds of formula II are used in the fluorination process according to the invention.
The process of the invention may carried out by dissolving or suspending compounds of formula II in a suitable solvent and cooling the solution to temperatures below 20° C. and preferably about 10° C. The amine salt, or approximately equivalent amounts of an amine and a strong acid, are then added, followed by the addition of the electrophilic fluorination agent. The fluorination is an exothermal reaction and care must be taken at this stage that the temperature does not exceed values which would effect and degrade compounds of formulae I and II. The fluorination agent is added preferably drop-wise or in portions and the reaction mixture is preferably cooled during this operation. The reaction mixture is stirred after the addition of the fluorination agent and the temperature may be increased to ambient values, preferably room temperature. The reaction progress can be controlled by the chromatographic determination of the starting material of formula I. The reaction is terminated when presence of the starting material can no longer be detected. The reaction time may be from 0.5 to 8 hours. The desired compounds of formula II can be isolated from the reaction mixture in known manner, for example in removing the solvent and filtering or extracting the resulting suspension, followed by re-crystallisation from a suitable solvent, for example from an alkanol.
The process according to the invention provides various advantages over prior art methods for the preparation of compounds of formula I, which are
a) a very high 6α/6β ratio of even higher than 99:1 in the crude reaction product;
b) reduced amounts of reaction by-products;
c) high chemical yields and short reaction times;
d) reduction of purification steps to obtain the desired 6α-fluorosteroid;
e) possibility of industrial scale manufacture;
The process according to the invention is very useful for the manufacture of fluorinated steorids, which are used as pharmaceutical effective compounds. Specific examples for such compounds are flumethasone, diflorasone, fluodinolone, difluprednate, and their derivatives.
The following examples illustrate the invention.
A) Preparation of Compounds of Formula II


EXAMPLE A1
Preparation of 9β,11-epoxy-16α-methyl-3,17,21-trihydroxy-pregna-1,3,5-triene-20-one-21-acetate-3-benzoate









To a solution of 110 grams of 9β,11β-epoxy-17,21-dihydroxy-16β-methyl-pregna-1,4-diene-3,20-dione-21-acetate in 275 grams of pyridine at 75° C. in a nitrogen atmosphere are added 66 grams of benzoyl chloride. The reaction mixture is then held at said temperature for 180 minutes, cooled to 30° C. and diluted with 55 grams of methanol. After stirring for 30 minutes at 45° C. the solution is added to a cold mixture of 160 grams of 85% phosphoric acid, 1100 grams of water, and 1100 grams of dichloromethane. After stirring for 30 minutes the organic phase is separated and again washed with 1100 grams of water. After separation, the organic phase is diluted with 11 grams of pyridine and evaporated under reduced pressure to an oil consisting of the title compound, which is directly used in the subsequent step. Triturafion of the oily residue with diisopropyl ether gives the title compound as a pale tan crystalline powder.
EXAMPLE A2
Preparation of 9β,11β-epoxy-16α-methyl-3,17,21-trihydroxy-pregna-1,3,5-triene-20-one-21-acetate-3-benzoate









To a solution of 108 grams of 9β,11β-epoxy-17,21-dihydroxy-16α-methyl-pregna-1,4-diene-3,20-dione-21-acetate in 216 grams of pyridine at 75° C. in a nitrogen atmosphere are added 64.8 grams of benzoyl chloride. The reaction mixture is then held at said temperature for 180 minutes, cooled to 30° C. and diluted with 54 grams of methanol. After stirring for 30 minutes at 45° C. the solution is added to a cold mixture of 126 grams of 85% phosphoric acid, 1080 grams of water, and 1080 grams of dichloromethane. After stirring for 30 minutes the organic phase is separated and again washed with 1080 grams of water. After separation, the organic phase is diluted with 10.8 grams of pyridine and evaporated under reduced pressure to an oil consisting of the title compound, which is directly used in the subsequent step.
EXAMPLE A3
Preparation of 9β,11β-epoxy-3,17,21-trihydroxy-pregna-1,3,5-triene-20-one-21-acetate-3-benzoate









To a solution of 50 grams of 9β,11β-epoxy-17,21-dihydroxy-pregna-1,4-diene-,3,20dione-21-acetate in 135 grams of pyridine at 75° C. in a nitrogen atmosphere are added 30 grams of benzoyl chloride. The reaction mixture is then held at said temperature for 180 minutes, cooled to 30° C. and diluted with 25 grams of methanol. After stirring for 30 minutes at 45° C. the solution is added to a cold mixture of 78 grams of 85% phosphoric acid, 500 grams of water, and 500 grams of dichioromethane. After stirring for 30 minutes the organic phase is sparated and again washed with 500 grams of water. After separation, the organic phase is diluted with 5 grams of pyridine and evaporated under reduced pressure to a crystalline residue consisting of the title compound, which is directly used in the subsequent step.
EXAMPLE A4
Preparation of 9β,11β-epoxy-3,16α,17,21-tetrahydroxy-pregna-1,3,5-triene-20-one-16,21-diacetate-3-benzoate









To a solution of 36 grams of 9β,11β-epoxy-16α,17,21-trihydroxy-pregna-1,4-diene-3,20-dione-16,21-diacetate in 72 grams of pyridine at 75° C. in a nitrogen atmosphere are added 21.6 grams of benzoyl chloride. The reaction mixture is then hold at said temperature for 180 minutes, cooled to 30° C. and diluted with 18 grams of methanol. After stirring for 30 minutes at 45° C. the solution is added to a cold mixture of 42 grams of 85% phosphoric acid, 360 grams of water, and 360 grams of dichloromethane. After stirring for 30 minutes the organic phase is separated and again washed with 360 grams of water. After separation, the organic phase is diluted with 3.6 grams of pyridine and evaporated under reduced pressure to an oil consisting of the title compound, which is directly used in the subsequent step.
EXAMPLE A5
Preparation of 9β,11β-epoxy-3,17,21-trihydroxy-16α-methyl-pregna-1,3,5-triene-20-one-3-benzoate-21-pivalate









To a solution of 40 grams of 9β,11β-epoxy-17,21-dihydroxy-16α-methyl-pregna-1,4-diene-3,20-dione-21-pivalate in 100 grams of pyridine at 75° C. in a nitrogen atmosphere are added 24 grams of benzoyl chloride. The reaction mixture is then held at said temperature for 300 minutes, cooled to 30° C. and diluted with 20 grams of methanol. After stirring for 30 minutes at 45° C. the solution is added to a cold mixture of 60 grams of 85% phosphoric acid, 400 grams of water, and 400 grams of dichloromethane. After stirring for 30 minutes the organic phase is separated and again washed with 400 grams of water. After separation, the organic phase is diluted with 4 grams of pyridine and evaporated under reduced pressure to an oil consisting of the title compound, which is directly used in the subsequent step
EXAMPLE A6
Preparation of 9β,11β-epoxy-16α-methyl-3,17,21-trihydroxy-pregna-1,3,5-triene-20-one-21-acetate-3-toluate









To a solution of 110 grams of 9β,11β-epoxy-17,21-dihydroxy-16α-methyl-pregna-1,4-diene-3,20-dione-21-acetate in 220 grams of pyridine at 75° C. in a nitrogen atmosphere are added 64.8 grams of p-methyl benzoyl chloride. The reaction mixture is then held at said temperature for 240 minutes, cooled to 30° C. and diluted with 55 grams of methanol. After stirring for 30 minutes at 45° C. the solution is added to a cold mixture of 129 grams of 85% phosphoric acid, 1100 grams of water, and 1100 grams of dichloromethane. After stirring for 30 minutes the organic phase is separated and again washed with 1100 grams of water. After separation, the organic phase is diluted with 11 grams of pyridine and evaporated under reduced pressure to an oil consisting of the title compound, which is directly used in the subsequent step.
B) Preparation of Compounds of Formula I
Example B1
Preparation of 9β,11β-epoxy-6α-fluoro-17,21-dihydroxy-16β-methyl-pregna-1,4-diene-3,20-dione-21-acetate
To a solution of the oily residue according to Example A1 in 957 grams of acetonitrile at 0° C. are added 22 grams of pyridine and 25.5 grams of methanesulfonic acid, followed by 95.7 grams of Selectfluor® added at such a rate that the temperature of the mixture does not exceed 5° C. The reaction mixture is then stirred at room temperature until HPLC analysis shows no starting compound remained. The HPLC analysis shows also that the resulting title compound contains only 1.0% of 6β-epimer(6α/6βratio is 99:1). The reaction mixture is diluted with 550 grams of water and then evoporated under reduced pressure to remove acetonitrile. Extraction of the resulting aqueous suspension using a mixture of dichloromethane and methanol (5:1 v/v) and subsequent crystallisation from methanol of the residue gives 91 grams of the pure title compound (HPLC analysis shows 0.69% of 6β-isomer).
EXAMPLE B2
Preparation of 9β,11β-epoxy-6α-fluoro-17,21-dihydroxy-16α-methyl-pregna-1,4-diene-3,20-dione-21-acetate









To a solution of the oily residue according to Example A2 in 940 grams of acetonitrile at 0° C. are added 21.6 grams of pyridine and 25 grams of methanesulfonic acid, followed by 94 grams of Selectfluor® added at such a rate that the temperature of the mixture does not exceed 5° C. The reaction mixture is then stirred at room temperature until HPLC analysis shows no starting compound remained. HPLC analysis shows also that the resulting title compound contains only 1.0% of 6β-epimer (6α/6β ratio is 99:1). The reaction mixture is diluted with 1080 grams of water and then evaporated under reduced pressure to remove acetonitrile. Extraction of the resulting aqueous suspension using a mixture of dichloromethane and methanol (5:1 v/v) and subsequent crystallisation from methanol of the residue gives 88 grams of the pure title compound.
EXAMPLE B3
Preparation of 9β,11β-epoxy-6α-fluoro-17,21-dihydroxy-pregna-1,4-diene-3,20-dione-21-acetate









To a solution of the oily residue according to Example A3 in 400 grams of acetonitrile at 0° C. are added 10 grams of pyridine and 11.8 grams of methanesulfonic acid, followed by 44.5 grams of Selectifluor® added at such a rate that the temperature of the mixture does not exceed 5° C. The reaction mixture is then stirred at room temperature until HPLC analysis shows no starting compound remained. HPLC analysis shows also that the resulting title compound contains less than 1.0% of 6β-epimer. The reaction mixture is diluted with 500 grams of water and then evaporated under reduced pressure to remove acetonitrile. Extraction of the resulting aqueous suspension using a mixture of dichloromethane and methanol (4:1 v/v) and subsequent crystallisation from methanol of the residue gives 40.5 grams of the pure title compound (HPLC analysis shows 0.95% of 6β-isomer).
EXAMPLE B4
Preparation of 9β,11β-epoxyo-6α-fluoro-16α,17,21-trihydroxy-pregna-1,4-diene-3,20-dione-16,21-acetate









To a mixture of 4.61 grams of methanesulfonic acid in 156 grams of acetonitrile, 4 grams of pyridine, and 3 grams of methyl benzoate was added at 0-5° C. 20 grams of crystalline 9β,11β-epoxy-3,16α,17,21-tetrahydroxy-pregna-1,3,5-triene-20-one-16,21-diacetate-3-benzoate obtained according to Example A4, followed by 12.6 grams of Selectlluor® added at such a rate that the temperature of the mixture does not exceed 5° C. The reaction mixture is then stirred at room temperature until HPLC analysis shows no starting compound remained. HPLC analysis shows also that the resulting title compound contains only 1.2% of 6β-epimer (6α/6β ratio is 99:1). The reaction mixture is diluted with 200 grams of water and then evaporated under reduced pressure to remove acetonitrile. Extraction of the resulting aqueous suspension using dichloromethane and subsequent crystallisation from diisopropyl ether of the residue gives 16.5 grams of the pure title compound (6β-epimer content 1.0%).
EXAMPLE B5
Preparation of 9β,11β-epoxy-6α-fluoro-17,21-dihydroxy-16α-methyl-pregna-1,4-diene-3,20-dione-21-pivalate









To a solution of the oily residue according to Example A5 in 400 grams of acetonitrile at 0° C. are added 4 grams of pyridine and 4.6 grams of methanesulfonic acid, followed by 31 grams of Selectfluor® added at such a rate that the temperature of the mixture does not exceed 5° C. The reaction mixture is then stirred at room temperature until HPLC analysis shows no starting compound remained. HPLC analysis shows also that the resulting title compound contains only 1.7% of 6β-epimer (6α/6β ratio is 98.3:1.7). The reaction mixture is diluted with 400 grams of water and then evaporated under reduced pressure to remove acetonitrile. Extraction of the resulting aqueous suspension using a mixture of dichloromethane and methanol (5:1 v/v) and subsequent crystallisation from methanol of the residue gives 29.5 grams of the pure title compound (HPLC analysis shows 0.35% of 6β-isomer).
EXAMPLE B6
Preparation of 9β,11β-epoxy-6α-fluoro-17,21-dihydroxy-16α-methyl-pregna-1,4-diene-3,20-dione-21-acetate









To a solution of the oily residue according to Example A6 in 960 grams of acetonitrile at 0° C. are added 22 grams of pyridine and 25.5 grams of methanesulfonic acid, followed by 95.7 grams of Selectfluor® added at such a rate that the temperature of the mixture does not exceed 5° C. The reaction mixture is then stirred at room temperature until HPLC analysis shows no starting compound remained. HPLC analysis shows also that the resulting title compound contains ca. 1.0% of 6β-epimer (6α/6βratio is 99:1). The reaction mixture is diluted with 1100 grams of water and then evaporated under reduced pressure to remove acetonitrile. Extraction of the resulting aqueous suspension using a mixture of dichloromethane and methanol (5:1 v/v) and subsequent crystallisation from methanol of the residue gives 87 grams of the pure title compound.