(en)The invention relates to a recombinant protein fabricated in a baculovirus system, of which the essential constitutive polypeptide sequence is that of a C-terminal fragment of 19 kilodalton (p19) of the surface protein 1 (protein MSP-1) of the merozoite parasite of the Plasmodium type, particularly Plasmodium falciparum, which is infectious for humans, said C-terminal fragment remaining normally anchored at the surface of the parasite at the end of its penetration phase into human erythrocytes, in the occurrence of an infectious cycle. Said recombinant protein is applicable to the production of vaccines against malaria.

1.ApplicationNumber: US-12503199-A
1.PublishNumber: US-6958235-B1
2.Date Publish: 20051025
3.Inventor: LONGACRE-ANDRE SHIRLEY
ROTH CHARLES
NATO FARIDABANO
BARNWELL JOHN W.
MENDIS KAMINI

4.Inventor Harmonized: LONGACRE-ANDRE SHIRLEY(FR)
ROTH CHARLES(FR)
NATO FARIDABANO(FR)
BARNWELL JOHN W(US)
MENDIS KAMINI(LK)

5.Country: US
6.Claims:
(en)The invention relates to a recombinant protein fabricated in a baculovirus system, of which the essential constitutive polypeptide sequence is that of a C-terminal fragment of 19 kilodalton (p19) of the surface protein 1 (protein MSP-1) of the merozoite parasite of the Plasmodium type, particularly Plasmodium falciparum, which is infectious for humans, said C-terminal fragment remaining normally anchored at the surface of the parasite at the end of its penetration phase into human erythrocytes, in the occurrence of an infectious cycle. Said recombinant protein is applicable to the production of vaccines against malaria.

7.Description:
(en)BACKGROUND OF THE INVENTION
1. Field of the Invention
The invention relates to novel active principles for vaccines derived from the major surface protein in merozoite forms of a Plasmodium which is infectious for mammals, especially humans, more generally termed MSP-1.
2. Discussion of the Background
MSP-1 has already been the subject of a number of studies. It is synthesised in the schizont stage of Plasmodium type parasites, in particular Plasmodium falciparum, and is expressed in the form of one of the major surface constituents of merozoites both in the hepatic stage and in the erthrocytic stage of malaria (1, 2, 3, 4). Because of the protein's predominant character and conservation in all known Plasmodium species, it has been suggested that it could be a candidate for constituting anti-material vaccines (5,6).
The same is true for fragments of that protein, particularly the natural cleavage products which are observed to form, for example during invasion by the parasite into erythrocytes of the infected host. Among such cleavage products are the C-terminal fragment with a molecular weight of 42 kDe (7, 8) which is itself cleaved once more into an N-terminal fragment with a conventional apparent molecular weight of 33 kDa and into a C-terminal fragment with a conventional apparent molecular weight of 19 kDa (9) which remains normally fixed to the parasite membrane after the modifications carried out on it, via glycosylphosphatidylinositol (GPI) groups (10, 11).
It is also found at the early ring stage of the intraerythrocytic development cycle (15, 18), whereby the observation was made that the 19 kDa fragment could play a role which is not yet known, but which is doubtless essential in re-invasive processes. This formed the basis for hypotheses formed in the past that that protein could constitute a particularly effective target for possible vaccines.
It should be understood that the references frequently made below to the p42 and p19 proteins from a certain type of Plasmodium are understood to refer to the corresponding C-terminal cleavage products of the MPS-1 protein of that Plasmodium or, by extension, to products containing substantially the same amino acid sequences, obtained by genetic recombination or by chemical synthesis using conventional techniques, for example using the “Applied System” synthesiser, or by “Merrifield” type solid phase synthesis. For convenience, references to “recombinant p42” and “recombinant p19” refer to “p42” and “p19” obtained by techniques comprising at least one genetic engineering step.
Faced with the difficulty of obtaining large quantities of parasites for P. falciparum and the impossibility of cultivating P. vivax in vitro, it has become clear that the only means of producing an anti-malaria vaccine is to resort to techniques which use recombinant proteins or peptides. However, MSP-1 is very difficult to produce whole because of it large size of about 200 kDa, a fact which has led researchers to study the C-terminal portion, the (still unknown) function of which is probably the more important.
Recombinant proteins concerning the C-terminal portion of the P. falciparum MSP-1 which have been produced and tested in the monkey (12, 40, 41) are:
a p19 fused with a glutathlone-S-transferase produced in E. coli (40); a p42 fused with a glutathione-S-transferase produced in E. coli (12); a p19 fused with a polypeptide from a tetanic anatoxin and carrying auxiliary T cell epitopes produced in S. cerevisiae (12); a p42 product in a baculovirus system (41).

A composition containing a p19 protein fused with a glutathione-S-transferase produced in E. coli combined with alum or liposomes did not exhibit a protective effect in any of six vaccinated Aotus nancymal monkeys (40).
A composition containing a p42 protein fused with a glutathione-S-transferase produced in E. coli combined with a Freund complete adjuvant did not exhibit a protective effect in two types of Aotus monkeys ( A. nancymal and A. vociferans ) when administered to them. The p19 protein produced in S. cerevisiae exhibited a protective effect in two A. nancymal type Aotus monkeys (12). In contrast, there was no protective effect in two A. vociferans type Aotus monkeys.
Some researchers (Chang et al.) have also reported immunisation tests carried out in the rabbit using a recombinant p42 protein produced in a baculovirus system and containing one amino acid sequence in common with P. falciparum (18). Thus these latter authors indicate that in the rabbit that recombinant p42 behaves substantially in the same way as the entire recombinant MSP-1 protein (gp195). This p42 protein in combination with a Freund complete adjuvant has been the subject matter of a vaccination test in a non-human primate susceptible to infection by P. falciparum, Aotus, lemurinus grisemembra (40). The results showed that 2 of 3 animals were completely protected and the third, while exhibiting a parasitemia which resembled that of the control, had a longer latent period. It is nevertheless risky to conclude to a protective nature in man of the antibodies thus induced against the parasites themselves. It should be remembered that there are currently no very satisfactory experimental models in the primate for P. vivax and P. falciparum. The Saimiri model, developed for P. falciparum and P. vivax, and the Aotus model for P. falciparum, are artificial systems requiring the parasite strains to be adapted and often requiring splenectomy of the animals to obtain significant parasitemia. As a result, the vaccination results from such models can only have a limited predictive value for man.
In any event, what the real vaccination rate would be which could possibly be obtained with such recombinant proteins is also questionable, bearing in mind the discovery—reported below—of the presence in p42s, from Plasmodiums of the same species, and more particularly in the corresponding p33s, of hypervariable regions which would in many cases render uncertain the immunoprotective efficacy of antibodies induced in individuals vaccinated with a p42 form a Plasmodium strain against an infection by other strains of the same species (13).
It can even be assumed that the high polymorphism of the N-terminal portion of p42 plays a significant role in immune escape, often observed for that type of parasite.
The aim of the present invention is to produce vaccinating recombinant proteins which can escape these difficulties, the protective effect of which is verifiable in genuinely significant experimental models or even directly in man.
More particularly, the invention provides vaccinating compositions against a Plasmodium type parasite which is infectious for man, containing as an active principle a recombinant protein which may or may not be glycosylated, whose essential constituent polypeptide sequence is:
either that of a 19 kilodalton (p19) C-terminal fragment of the surface protein 1 of the merozoite form (MSP-1 protein) of a Plasmodium type parasite which is infectious for man, said C-terminal fragment remaining normally anchored to the parasite surface at the end of its penetration phase into human erythrocytes in the event of an infectious cycle; or that of a portion of that fragment which is also capable of inducing an immune response which can inhibit in vivo parasilemia due to the corresponding parasite; or that of an immunologically equivalent peptide of said p19 fragment or said portion of that fragment; and

said recombinant protein further comprises conformational epitopes which are unstable in a reducing medium and which preferably constitute the majority of the epitopes recognised by human antiserums formed against the corresponding Plasmodium.





BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1A illustrates the nucleotide and amino acid sequences of the synthetic gene (Bac 19) and the “native gene” (PF19) of P. falciparum described by Chang et al;
FIG. 1B illustrates the nucleotide and amino acid sequences of the synthetic gene (Bac 19) and the “native gene” (PF19) of the Uganda Palo Alto isolate of P. falciparum;
FIG. 1C illustrates the PfMSP1 P19 A recombinant protein sequence before cutting out the signal;
FIG. 1D illustrates the PfMSP1 P19 A recombinant protein after cutting out the signal sequence;
FIG. 2A is an immunoblot using SDS-PAGE of the soluble recombinant PfMSP1 P19 antigen purified by immunoaffinity of the presence (reduced) or absence (non-reduced) of β-mercaptoethanol;
FIG. 2B is an immunoblot with human antiserum of recombinant purified MSP-1 P19 from P. vivax and P. cynomoigi under non-reduced (NR), reduced only in the charging medium (R) and irreversibly reduced (IR) conditions;
FIG. 3A is an immunoblot of the soluble PvMSP1 P42 recombinant antigen in the presence of protein fractions derived from merzoites of P. falciparum and separately isoelectric focusing in the presence (reduced) or absence (nonreduced) of β-mercaptoethanol;
FIG. 3B is a graph illustrating the results of an ELISA inhibition technique of P. vivax MPS-1 P42 and P12 antigens by the antiserum of individuals with an acquired immunity to P. vivax;
FIG. 4 are nucleotide sequences. The underlines oligonucleotides originate from P. vivax and are used as primers in a PCR reaction. The lower portion of FIG. 4 illustrates the percent identity between two isolates of P. vivax and P. cynomoigi;
FIG. 5 are curves illustrating the variation in the measured parasitemia as the number of parasited red blood cells per microliter of blood as the function of time passed after infection. Curve A corresponds to the average values observed in three vaccinated monkeys and curve B corresponds to the average values in five controls;
FIG. 6A is a graph illustrating the parasitemia observed in non-vaccinated control animals as a function of time after injection;
FIG. 6B is a graph illustrating the parasitemia observed in control animals which contained a saline solution also contain Freunds adjuvant as a function of time after injection;
FIG. 6C is a superposition of FIGS. 6A and 6B ;
FIG. 6D is a graph illustrating parasitemia at the end of vaccination with p42 as a function of time;
FIG. 6E is a graph illustrating parasitemia in animals vaccinated with p19 alone as a function of time;
FIG. 6F is a graph illustrating parasitemia in animals with a mixture of P42 and P19 as a function of time;
FIG. 6G is the data obtained to produce the graphs in FIGS. 6A to 6 F;
FIG. 7A is an immunoblot illustrating the in vivo response of monkeys to injections of p19 with Freunds adjuvant (1), with alum (2) and in the form of liposomes (3);
FIG. 7B is an immunoblot illustrating the in vitro responses of a squirrel monkey after three injections with p19 with Freunds adjuvant, with alum and in the form of liposomes;
FIG. 8A is a graph illustrating the percent parasitemia versus days post infection of six monkeys which were immunized with recombinant MSP-1(p19) six months earlier;
FIG. 8B is a graph illustrating the percent parasitemia versus days post infection of six monkeys that were immunized with normal saline and an adjuvant;
FIG. 8C is a graph illustrating the percent parasitemia versus days post infection of monkeys that were used as controls;
FIG. 8D is the data obtained to produce the graphs of FIGS. 8A to 8 C;
FIG. 9A is a graph illustrating the percent parasitemia versus days post infection of 2 macaques immunized with recombinant p19 and alum;
FIG. 9B is a graph illustrating the percent parasitemia versus days post infection of 2 macaques immunized with recombinant p19 and alum;
FIG. 9C is a graph illustrating the percent parasitemia versus days post infection of a macaque immunized with p19;
FIG. 9D is a graph illustrating the percent parasitemia versus days post infection of 3 control macaques immunized with physiological water and alum;
FIG. 9E is the data obtained to generate the graphs in FIGS. 9A to 9 D;
FIG. 10A is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with MPS-1 p19 and alum;
FIG. 10B is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with MSP-1 p19 and Freunds;
FIG. 10C is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with MSP-1 p19 with liposomes;
FIG. 10D is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with alum as the control;
FIG. 10E is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with Freunds as the control;
FIG. 10F is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with liposomes as the control;
FIG. 10G is a graph illustrating the percent parasitemia versus days post infection in a squirrel monkey immunized with physiological water as the control.



DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS
The presence of such conformational epitopes could play an important role in the protective efficacy of the active principle of the vaccines. They are particularly found in the active principles which exhibit the other characteristics defined above, when they are produced in a baculovirus system. If needs be, it is mentioned below that the expression “baculovirus vector system” means the ensemble constituted by the baculovirus type vector itself and the cell lines, in particular cells of insects transfectable by a baculovirus modified by a sequence to be transferred to these cell lines resulting in expression of that transferred sequence. Preferred examples of these two partners in the baculovirus system have been described in the article by Longacre et al. (19). The same system was used in the examples below. It goes without saying, of course, that variations in the baculovirus and in the cells which can be infected by the baculovirus can be used in place of those selected.
In particular, the recombinant protein is recognised by human antiserums formed against the corresponding Plasmodium or against a homologous Plasmodium when it is in its non reduced state or in a reduced non irreversible state, but it not recognised or is only recognised to a slight extent by these same antiserums when it is irreversibly reduced.
The unstable character of these conformational epitopes in a reducing medium can be demonstrated by the test described below in the examples, in particular in the presence of β-mercaptoethanol. Similarly, the examples below describe the experimental conditions applicable to obtain irreversible reduction of the proteins of the invention.
From this viewpoint, the recombinant protein produced by Longacre et al. (14) can be used in such compositions. It should be remembered that S. Longacre et al. succeeded in producing a recombinant p19 from the MSP-1 of P. vivax in a baculovirus vector system containing a nucleotide sequence coding for the p19 of Plasmodium vivax, in particular by transfecting cultures of insect cells [ Spodoptera frugiperda (Sf9) line] with baculovirus vectors containing, under the control of the polyhedrin promoter, a sequence coding for the peptide sequences defined below, with the sequences being placed in the following order in the baculovirus vector used;
a 35 base pair 5′ terminal fragment of the polyhedrin signal sequence, in which the methionine codon for initiating expression of this protein had been mutated (to ATT); a 5′-terminal nucleotide fragment coding for a 32 amino acid peptide corresponding to the N-terminal portion of MSP-1, including the MSP-1 signal peptide; either a nucleotide sequence coding for p19, or a sequence coding for the p42 of the MSP-1 protein of Plasmodium vivax, depending on the case, these sequences also being provided with (“anchored” forms) or deprived of (soluble forms) 3′ end regions of these nucleotide sequences, whose end C-terminal expression products are reputed to play an essential role in anchoring the final p19 protein to the parasite membrane; 2 TAA stop codons.

For p42, the sequences derived from the C-terminal region of MSP-1 extend consequently from amino acid Asp 1325 to amino acid Leu 1726 (anchored form) or to amino acid Ser 1705 (soluble form) and for p19, the sequences extend from amino acid IIe 1602 to amino acid Leu 1726 (anchored form) or to amino acid Ser 1705 (soluble form) it being understood that the complete amino acid sequences of p42 and p19, whose initial and terminal amino acids have been indicated above follow from the gene of the Belem isolate of P. vivax which has been sequenced (20).
Similar results were obtained using, in the same vector systems, nucleotide sequences coding for the p42 and p19 of Plasmodium cynomoigi. The interest in P. cynomoigi is twofold; it is a parasitic species very close to P. vivax which is infectious for the macaque. It can also infect man. Further, access to the natural hosts of P. cynomoigi, rhesus monkeys and toque macaques, is also possible, to test the efficacy of the protection of MSP-1 from P. cynomoigi in natural systems. The rhesus monkey is considered to be one of the most representative species for immune reactions in man.
In particular, excellent results have been obtained in vaccination tests carried out using the toque macaque with two recombinant polypeptides: soluble p42 and, in particular, soluble p19 derived from P. cynomoigi, respectively produced in a baculovirus system and purified on an affinity column with monoclonal antibodies recognising the corresponding regions of the native MSP-1 protein. The following observations were made: six monkeys immunised with only p19 (three monkeys) and the p19 and p42 together (three monkeys) all exhibited practically sterile immunity after challenge infection. The results obtained in the three monkeys immunised with p42 were less significant. Two of them were as above, but since the third exhibited a lower parasitemia than the controls immunised with a PBS buffer in the presence of Freund adjuvant (3 monkeys) or not immunised (3 monkeys), it was less clear.
A second challenge infection showed that the monkeys which had received p19 alone were protected for at least six months. A second vaccination test with p19 in combination with alum in this system (toque macaque P. cynomoigi ) exhibited significant protection for 2 of the 3 monkeys. This is the first time that MSP-1 or another recombinant antigen has demonstrated a protective effect in the presence of alum (42).
The particularly effective test results carried out with the macaque with recombinant polypeptides produced in a baculovirus system using a recombinant p19 from P. cynomoigi showed that recombinant polypeptides respectively containing recombinant p19s from other Plasmodiums must behave in the same manner. They are more meaningful for malaria in man than the results from tests carried out with P. vivax or P. falciparum in their “artifical hosts”.
Baculovirus recombinant proteins derived from a C-terminal MSP-1 portion (p19) have a very significant antimalarial protective effect in a natural system, which constitutes the most representative model for evaluating the protective effect of MSP-1 for man.
The protective effect obtained can be further improved if the p19 form is deprived of the hypervariable region of the N-terminal portion of p42, the effect of which can be deleterious in natural situations in which the vaccinated subject is confronted by a great deal of polymorphism. Further, p19 appears to possess specific epitopes which are not present in p42.
The 19 kDa C-terminal fragment, the sequence of which is present in the active principle of the vaccine, can be limited to the sequence for the p19 itself, in the absence of any polypeptide sequence normally upstream of the p19 sequence in the corresponding MSP-1 protein. Clearly, though, the essential constituent polypeptide sequence of the active principle can also comprise a polypeptide sequence for the C-terminal side belonging to the 33 kDa (p33) N-terminal fragment still associated with the p19 in the corresponding p42, before natural cleavage of the latter, if the presence of this fragment does not modify the immunological properties of the active principle of the vaccine. As will be seen below, in particular in the description of the examples, the C-terminal sequences of the p33 in various strains of the same species of Plasmodium (see the C-terminal portion of the peptide sequences of “region III” in FIG. 4 (SEQ ID NOS:11-14) also have a degree of homology or substantial conservation of the sequence, for example of the order of at least 80%, in different varieties of Plasmodiums which are infectious for man, such that they do not fundamentally modify the vaccinating properties of the active principle (the sequence of which corresponds to region IV in FIG. 4 ), in particular using the hypothesis which follows from this figure; that the presumed cleavage site between the p19 and region III of the p33 is located between the leucine and asparagine residues in a particularly well conserved region (LNVQTQ) (SEQ ID NO:15).
Normally the C-terminal polypeptide sequence of the p33, when it is present, comprises less than 50 amino acid residues, or even less than 35, preferably less than 10 amino acid residues.
In contrast, the essential constituent polypeptide sequence of the active principle of the vaccine need not comprise all of the sequence coding for p19, naturally providing that the latter retains the ability to induce antibodies which protect against the parasite. In particular, the molecular weight of the “fragment portion” is 10 to 25 kDa, in particular 10 to 15 kDa. Preferably, this polypeptide fragment portion contains at least one of the two EGF (Epidermal Growth Factor) regions.
Clearly, the skilled person could distinguish between active fragments and those which would no longer be so, in particular experimentally by producing modified vectors containing inserts with different lengths originating from the p19, respectively isolated from the fragments obtained from the sequence coding for p19, by reaction with appropriate restriction enzymes, or by exonucleolytic enzymes which would be kept in contact with the fragment coding for p19 for differing periods; the capacity of the expression products from these inserts in the corresponding eukaryotic cells, in particular in insect cells, transformed by the corresponding modified vectors, to exert a protective effect can then be tested, in particular under the experimental conditions which are described below in the examples. In particular, the expression products of these inserts must be able to inhibit a parasitemia induced in vivo by the corresponding whole parasite.
Thus, the invention includes all vaccinating compositions in which the essential constituent polypeptide sequence of the active principle is constituted by a peptide which can induce a cellular and/or humoral type immunological response equivalent to that produced by p19 or a fragment as defined above, provided that the addition, deletion or substitution in the sequence of certain amino acids by others would not cause a large modification of the capacity of the modified peptide—hereinafter termed the “immunologically equivalent peptide”—to inhibit said parasitemia.
The p19 fragment can naturally also be associated at the N-terminal side or the C-terminal side or via a peptide bond to a further plasmoidal protein fragment having a vaccinating potential (such as Duffy binding protein from P. vivax (29) or EBA-175 from P. falciparum (30) and (31), one region of which is specifically rich in cysteine), provided that its capacity to inhibit parasitemia normally introduced in vivo by the corresponding parasite is not altered but is amplified.
Upstream of the N-terminal end of p19, the fragment coding for p19 or a portion thereof can also contain a peptide sequence which is different again, for example a C-terminal fragment of the signal peptide used, such as that for the MSP-1 protein. This sequence preferably comprises less than 50 amino acids, for example 10 to 40 amino acids.
These observations pertain in similar fashion to the p19s from other Plasmodium, in particular P. falciparum, the dominant species of the parasites, responsible for one of the most serious forms of malaria.
However, the techniques summarised above for producing a recombinant p19 from P. vivax or P. cynomoigi in a baculovirus system are difficult to transpose unchanged to producing a recombinant p19 of P. falciparum in a satisfactory yield, if only to obtain appreciable quantities which will allow immunoprotective tests to be carried out.
The invention also provides a process which overcomes this problem to a large extent. It also becomes possible to obtain much higher yields of P. falciparum p19—and other Plasmodiums where similar difficulties are encountered—using a synthetic nucleotide sequence substituting the natural nucleotide sequence coding for the p19 of Plasmodium falciparum in an expression vector of a baculovirus system, this synthetic nucleotide sequence coding for the same p19, but being characterized by a higher proportion of G and C nucleotides than in the natural nucleotide sequence.
In other words, the invention follows from the discovery that expression of a nucleotide sequence coding for a p19 in a baculovirus system is apparently linked to an improved compatibility of successive codons in the nucleotide sequence to express with the “cellular machinery” of the host cells transformable by the baculovirus, in the manner of that observed for the natural nucleotide sequences normally contained in these baculovirus and expressed in the infected host cells; hence the poor expression, or even total absence of expression of a native P. falciparum nucleotide sequence; hence also a possible explanation of the more effective expression observed by Longacre et al. (14) for the p19 of P. vivax in a baculovirus system and, as the inventors have also shown, of the P. cynomoigi sequence from corresponding native p19 nucleotide sequences, because of their relatively much higher amounts of G and C nucleotides than those of the native nucleotide sequences coding for the p19 of P. falciparum.
The invention thus more generally provides a recombinant baculovirus type modified vector containing, under the control of a promoter contained in said vector and able to be recognised by cells transfectable by said vector, a first nucleotide sequence coding for a signal peptide exploitable by a baculovirus system, characterized by a second nucleotide sequence downstream of the first, also under the control of the promoter and coding for the peptide sequence;
either of a 19 kilodalton (p19) C-terminal fragment of the surface protein 1 of the merozoite form (MSP-1protein) of a Plasmodium type parasite other than Plasmodium vivax which is infectious for man, said C-terminal fragment remaining normally anchored to the parasite surface at the end of its penetration phase into human erythrocytes in the event of an infectious cycle; or of a portion of that peptide fragment provided that the expression product from the second sequence in a baculovirus system is also capable of inducing an immune response which can inhibit in vivo parasitemia due to the corresponding parasite; or of an immunologically equivalent peptide of said C-terminal peptide fragment (p19) or said peptide fragment portion by addition, deletion or substitution of amino acids not resulting in a large modification of the capacity of said immunologically equivalent peptide to induce a cellular and/or humoral type immunological response similar to that produced by said p19 peptide fragment or said portion of said fragment; and

said nucleotide sequence having, if necessary, a G and C nucleotide content in the range 40% to 60%, preferably at least 50%, of the totality of the nucleotides from which it is constituted. This sequence can be obtained by constructing a synthetic gene in which the natural codons have been changed for codons which are rich in G/C without modifying their translation (maintaining the peptide sequence).


The nucleotide sequence, provided by a synthetic DNA, may have at least 10% of modified codons with respect of the natural gene sequence or cDNA while retaining the characteristics of the natural translated sequence, i.e., maintaining the amino acid sequence.
It is not excluded that this G and C nucleotide content could be further increased provided that the modifications resulting therefrom as to the amino acid sequence of the recombinant peptide—or immunologically equivalent peptide—produced do not result in a loss of immunological properties, or protective properties, of the recombinant proteins formed, in particular in the tests which will be described below.
These observations naturally apply to other Plasmodium which are infectious for man, in particular those where the native nucleotide sequences coding for corresponding p19s would have T and A nucleotide contents which are poorly compatible with effective expression in a baculovirus system.
The sequence coding for the signal used can be that normally associated with the native sequence of the Plasmodium concerned. But it can also originate from another Plasmodium, for example P. vivax or P. cynomoigi or another organism if it can be recognised as a signal in a baculovirus system.
The sequence coding for p19 or a fragment thereof in the vector under consideration is, in one case, deprived of the anchoring sequence of the native protein to the parasite from which it originates, in which case the expressed protein is generally excreted into the culture medium (soluble form). It is also remarkable in this respect that under the conditions of the invention, the soluble and anchored forms of the recombinant proteins produced, in particular when they are from P. falciparum or P. cynomoigi or P. vivax, tend to form oligomers, this property possibly being at the origin of the increased immunogenicity of the recombinant proteins formed.
The invention also concerns vectors in which the coding sequence contains the terminal 3′end sequence coding for the hydrophobic C′-terminal end sequence of the p19 which is normally implicated in the induction of anchoring the native protein to the cell membrane of the host in which it is expressed. This 3′-terminal end region can also be heterologous as regards the sequence coding for the soluble p19 portion, for example corresponding to the 3′-terminal sequence from P. vivax or from another organism when it codes for a sequence which anchors the whole of the recombinant protein produced to the cell membrane of the host of the baculovirus system used. An example of such anchoring sequences is the GPI of the CD59 antigen which can be expressed in the cells of Spodoptera frugiperda (32) type insects or the GPI of a CD14 human protein (33).
The invention also, naturally , concerns recombinant proteins, these proteins comprising conformational epitopes recognised by human serums formed against the corresponding Plasmodium.
In general, the invention also concerns any recombinant protein of the type indicated above, provided that it comprises conformational epitopes such as those produced in the baculovirus system, in particular those which are unstable in a reducing medium.
The invention also, naturally, concerns said recombinant proteins, whether they are in their soluble form or in the form provided with an anchoring region, in particular to cellular hosts used in the baculovirus system.
The invention also encompasses oligomers spontaneously produced in the baculovirus systems used or produced is posteriori, using conventional protein oligomerisation techniques. The most commonly used technique involves glutaraldehyde. However, any conventional system for bridging between the respective amine and carboxyl functions in proteins can be used. As an example, any of the techniques described in European patent application EP-A-0 602 079 can be used.
The term “oligomer” means a molecule containing 2 to 50 monomer units, each of the monomer units containing p19 or a fragment thereof, as defined above, capable of forming an aggregate. The invention also encompasses any conjugation product between a p19 or a p19 fragment as defined above, and a carrier molecule—for example a polylysine-alanine—for use in producing vaccines, via bonds which are covalent or otherwise. The vaccinating compositions using them also form part of the invention.
The invention still further concerns vaccine compositions using these oligomeric or conjugated recombinant proteins, including proteins from Plasmodium vivax, these observations also extending to oligomers of these recombinant proteins.
The invention also encompasses compositions in which the recombinant proteins defined above are associated with an adjuvant, for example an alum. Recombinant proteins containing the C-terminal end region allowing them to anchor to the membrane of the cells in which they are produced are advantageously used in combination with lipids which can form liposomes appropriate to the production of vaccines. Without being limiting, lipids described, for example, in the publication entitled “Les liposomes aspects technologique, biologique et pharmacologique” [Liposomes: technological, biological and pharmacological aspects] by J. Delattre et al., INSERM, 1993, can be used.
The presence of the anchoring region in the recombinant protein, whether it is a homologous or heterologous anchoring region as regards the vaccinating portion proper, encourages the production of cytophilic antibodies, in particular IgG 2a and IgG 2b type in the mouse which could have a particularly high protective activity, so that associating the active principles of the vaccines so constituted with adjuvants other than the lipids used to constitute the liposome forms could be dispensed with. This amounts to a major advantage, since liposomes can be lyophilised under conditions which enable them to be stored and transported, without the need for chains of cold storage means.
Other characteristics of the invention will become clear from the following description of examples of recombinant proteins of the invention and the conditions under which they can be produced. These examples are not intended to limit the scope of the invention.
Description of the Construction of PfMSP1 p19 S (soluble) (soluble p19 from P. falciparum )
The recombinant construction PfMSP1 p19 S contains the DNA corresponding to 8 base pairs of the leader sequence and the first 32 amino acids of the MSP-1 of Plasmodium vivax from Met 1 to Asp 32 (Belem isolate; Del Portillo et al., 1991, P.N.A.S., 86, 4030) followed by GluPhe due to the EcoR1 site connecting the two fragments. This followed by the synthetic gene described in FIG. 1 , coding the Plasmodium falciparum MSP1 p19 from Asn 1613 to Ser 1705 (Uganda-Palo Alto isolate; Chang et al., 1988, Exp. Parasitol. 67. 1). The construction is terminated by two TAA stop codons. This construction gave rise to a recombinant protein which was secreted in the culture supernatant from infected cells.
In the same manner and for comparison, a recombinant construction was produced under conditions which were similar to those used to produce the p19 above, but working with a coding sequence consisting of a direct copy of the corresponding DNA of the P. falciparum strain (FUP) described by Chang et al., Exp. Parasit. 67,1; 1989. The natural gene copy (from asparagine 1613 to serine 1705) was formed from the native gene by PCR.
FIG. 1A shows the sequences of both the synthetic gene (Bac19) (SEQ ID NO.1) and the “native gene” (PF19) (SEQ ID NO:3).
It can be seen that 57 codons of the 93 codons of the native sequence coding for the p19 from P. falciparum were modified (the third nucleotide in 55 of them and the first and third nucleotides in the other 2 codons). New codons were added to the 5′ end to introduce the peptide signal under the conditions indicated above and to introduce an EcoRl site for cloning, and similarly two stop codons were added which were not present in the P. falciparum p19 to obtain expression termination signals. The individual letters placed above successive codons correspond to the respective successive amino acids. Asterisks (*) show the stop codons. Vertical lines indicate the nucleotides which are the same in the two sequences.
Description of the PfMSP1 p19 A construction (anchored GPI) (anchored p19 of P. falciparum )
The PfMSP1 p19 A construction had the characteristics of that above except that the synthetic sequence ( FIG. 1B ) (SEQ ID NOS;4and 6) codes for the MSP1 p19 of Plasmodium falciparum (Uganda-Palo Alto isolate) from Asn 1613 to Ile 1726 followed by two TAA stop codons. This construction gave rise to a recombinant protein which was anchored in the plasma membrane of infected cells by a glycosyl phosphatidyl inositol (GPI) type structure.
FIG. 1C represents the PfMSP1 p19 S recombinant protein sequence before cutting out the signal sequence (SEQ ID NO:7).
FIG. 1D represents the PfMSP1 p19 S recombinant protein sequence after cutting out the signal sequence (SEQ ID NO:9).
The amino acids underlined in FIGS. 1C and 1D originate from the EcoR1 site used to join the nucleotide sequences derived from the N-terminal portion of the MSP-1 of P. vivax (with signal sequence) and the MSP-1 p19 of P. falciparum.
FIG. 2 —The soluble recombinant PfMSP1 p19 antigen purified by immunoaffinity was analysed by immunoblot using SDS-PAGE in the presence (reduced) or absence (non reduced) of β-mercaptoethanol. Samples were charged onto gel after heating to 95° C. in the presence of 2% SDS. Under these conditions only covalent type bonds (disulphide bridges) can resist disaggregation. The left hand blot was revealed with a monoclonal antibody which reacted with a linear epitope of natural p19. The right hand blot was revealed with a mixture of 13 human antisera originating from subjects with acquired immunity to malaria due to Plasmodium falciparum. These results show that the recombinant baculovirus molecule can reproduce conformational epitopes in the form of a polymer the majority of which are recognised by human antiserum.
FIG. 2 B: Immunoblot analysis with human antiserum of recombinant purified MSP-1 p19 from P. vivax and P. cynomoigi under non reduced (NR), reduced only in the charging medium (R) and irreversibly reduced (IR) conditions
This work was based on the idea that the baculovirus expression system correctly reproduced the conformational epitopes present in vivo on the C-terminal portion of MSP-1 in large amounts. The best means of measuring this property (which may be the only possible means in the absence of native purified proteins corresponding to p19) was to study the reactivity of the recombinant proteins with the antiserum of individuals exposed to malaria, this reflecting the native proteins as “seen” by the human immune system.
Thus soluble recombinant PvMSP-1 p19 and PcMSP-1 p19 antigens purified by immunoaffinity were analysed by immunoblot using SDS-PAGE (15%) in the presence (reduced) or absence (non reduced) of DTT. Samples were charged onto gel after heating to 95° C. in the presence of 2% SDS. The irreversible reduction was carried out as follows: the protein was resuspended in 0.2 M Tris-HCl, pH 8.4, 100 mM DTT, 1.0% SDS and heated for 30 minutes at 70° C. After diluting with water, acrylamide was added to a final concentration of 2 M and the mixture was incubated under nitrogen in the dark, for 1 hour at 37° C. the immunoblot was revealed with a mixture of 25 human antisera originating from subjects with an acquired immunity to malaria due to Plasmodium vivax. V and C respectively designate proteins derived from the MSP-1 of P. vivax and P. cynomoigi. It should be noted that irreversibly reduced recombinant proteins exhibited no reactivity with human antiserum while non irreversibly reduced proteins or non reduced proteins exhibited good reactivity. (The non reduced Pv MSP-1 p19 was a little weak since in its glycosylated state it does not bind well to nitro-cellulose paper). These results show that recognition of baculovirus MSP-1 p19 molecules by human antiserum is largely if not completely dependent on conformational epitopes sensitive to reduction which are reproduced in this system.
FIG. 3 —The soluble PvMSP1 p42 recombinant antigen (Longacre et al., 1994, op. Cit.) was incubated for 5 hours at 37° C. in the presence of protein fractions derived from merozoites of P. falciparum and separated by isolectrofocussing. The samples were then analysed by immunoblot in the presence (reduced) or absence (non reduced) of β-mercaptoethanol, isolectrofocussing fractions 5 to 12, and two total merozoite extracts made in the presence (Tex) or absence (T) of detergent, were analysed. The immunoblot was revealed with monoclonal antibodies specific for MSP1 p42 and p19 of P. vivax. The results suggest that there is a proteolytic activity in the P. falciparum merozoites which can be extracted with detergent. Digestion of p42 in certain fractions appear to cause polymerisation of the digestion products (p19); this polymerisation is probably linked to the formation of disulphide bridges since in the presence of β-mercaptoethanol, the high molecular weight forms disappear in favour of a molecule of about 19 kDa (Tex-R). The p19 polymerisation observed in these experiments could thus be an intrinsic property of this molecule in vivo.
FIG. 3 B: The differential contribution of p42 and p19 antigens to the P. vivax anti-MSP-1 human response
Recognition of P. vivax MSP-1 p42 and p19 antigens by the antiserum of individuals with an acquired immunity to P. vivax was compared using the ELISA inhibition technique as follows: a mixture of 25 human antisera originating from subjects with an acquired immunity to malaria due to P. vivax was diluted to 1:5000 and incubated for 4 hours at ambient temperature either alone, or in the presence of a 1 mM purified P. vivax recombinant p42 or p19. This mixture was transferred to a microliter well which had been coated for 18 hours at 4° C. with 500 ng.ml −1 of purified absorbed recombinant p42 or p19, and incubated for 30 minutes at ambient temperature. After washing with PBS containing 0.1% of Tween 20, a goat anti-mouse IgG conjugated with peroxidase was added and the mixture was incubated for 1 hour at 37° C. The enzymatic activity was revealed by reading the optical density at 492 nm. The percentage inhibition was calculated based on values of 100% of antiserum activity with the coated antiserum on the microtitre plate in the absence of a competing antigen. Statistical data were calculated using a Statview program. Each bar represents the average percentage inhibition of a pair of competing/absorbed antigens based on 4 to 12 determinations; the vertical lines correspond to a 95% confidence interval. Asterisks (*) designate the antigens produced in the presence of tunicamycin, thus with no N-glycosylation. The important parameters of these measurements were the dilution of the antiserum by 1:5000 which is in the region which is sensitive to ELISA curves and the competing antigen concentrations of 1 mM which includes competition by low affinity epitopes. Thus these data reflect the maximum resemblance between the two compared antigens. The results show that the majority, if not all of the p42 epitopes recognised by the human antiserum are present on the p19 since in the presence of the latter, the reactivity of the human antiserum against p42 is inhibited as much as by the p42 antigen itself. In contrast, however, about 20% of the p19 epitopes recognised by human antiserum were not or were not accessible on the p42, since the reactivity of the human antiserum against the p19 was much less inhibited by p42 than by p19 itself. Such specific epitopes of p19 can be constituted or revealed only after cleaving the p42 into p19 and p33. These results were not affected by glycosylation showing that the effect is really due to a difference between the peptide components of p19 and p42 and not to a difference in glycosylation. These results underline the fact that p19 has a distinct immunological identity to p42.
Description of the PcMSP1 p19 S (Soluble) Construction (Soluble p19 of P. cynomolgi )
The DNA used for the above construction was obtained from a clone of the Plasmodium cynomolgi ceylonesis strain (22-23). This strain had been maintained by successive passages through its natural host ( Macaca sinica ) and cyclic transmissions via mosquitoes (27).
Blood parasites in the mature schizont stage were obtained from infected monkeys when the parasitemia had attained a level of 5%. They were then purified using the methods described in (25). The DNA was then extracted as described in (26).
A 1200 base pair fragment was produced using a PCR reaction using the oligonucleotides underlined in FIG. 4 originating from P. vivax. The 5′ oligonucleotide comprised of EcoRI restriction site and the 3′ oligonucleotide comprised two synthetic TAA stop codons followed by a BgIII restriction site. This fragment was introduced by ligation and via these EcoRI and BgIII sites into the pVLSV 200 plasmid already containing the signal sequence for the MSP-1 protein of P. vivax (19). The new plasmid (pVLSV 200 C 42 ) was used to analyse the DNA sequences.
The P. cynomolgi and the corresponding P.vivax sequences were aligned (SEQ ID NOS:11-14). The black arrows designate the presumed primary and secondary cleavage sites. They were determined by analogy with known sites in P. falciparum (27, 28). The vertical lines and horizontal arrows localise the limits of the four regions which were studied. Region 4 corresponded to the sequence coding for the P. cynomolgi p19. Glycosylation sites are boxed and the preserved cysteines are underlined. The lower portion of FIG. 4 shows the percentage identity between the two isolates of P. vivax and P. cynomolgi.
The recombinant construction PcMSP1 p19 S contains the DNA corresponding to 8 base pairs of the leader sequence and the first 32 amino acids of the MSP-1 of Plasmodium vivax from Met 1 to Asp 32 (Belem isolate; Del Portillo et al. 1991, P. N. A. S., 88, 4030) followed by GluPhe, due to the EcoR1 site, connecting the two fragments. This is followed by the sequence coding for the Plasmodium cynomolgi MSP1 p19 from Lys 276 to Ser 380 (Ceylon strain). The construction was terminated by two TAA stop codons. This construction gave rise to a recombinant protein which was secreted in the culture supernatant of infected cells.
Purification of Recombinant PfMSP1p19 Protein by Immunoaffinity Chromatography with a Monoclonal Antibody Specifically Recognising the p19 of Plasmodium falciparum
The chromatographic resin was prepared by binding 70 mg of a monoclonal antibody (obtained from a G17.12 hybridoma deposited at the CNCM [National Collection of Microorganism Cultures] (Paris, France) on the 14 th Feb. 1997, registration number I-1846; this G17.12 hybridoma was constructed from X63 Ag8 653 myeloma producing IgG 2a/k recognising the P. falciparum p19) to 3 g of activated CNBr-Sepharose 4B (Pharmacia) using standard methods detailed in the procedure employed by Pharmacia. The culture supernatants containing the soluble PfMSP1p19 were batch incubated with the chromatographic resin for 16 hours at 4° C. The column was washed once with 20 volumes of 0.05% NP40, 0.5 M of NaCl, PBS; once with 5 volumes of PBS and once with 2 volumes of 10 mM of sodium phosphate, pH 6.8. Elution was carried out with 30 ml of 0.2 M glycine, pH 2.2. The eluate was neutralised with 1 M sodium phosphate, pH 7.7 then concentrated by ultrafiltration and dialysed against PBS. To purify the anchored PfMSP1p19, all of the washing and elution solution contained a supplemental 0.1% of 3-(dimethyl-dodecylammonio)-propane sulphonate (Fluka).
Recombinant Plasmodium vivax (p42 and p19) MSP1 Vaccination Test in the Squirrel Monkey Saimiri solureus
This vaccination test was carried out on male non splenectomised 2 to 3 year old Saimiri sclureus boliviensis monkeys. Three monkeys were injected 3 times intramuscularly at 3 week intervals with a mixture of about 50 to 100 μg each of recombinant soluble PvMSP1 p42 and p19 (19), purified by immunoaffinity. Complete and incomplete Freund adjuvant was used as follows: 1 st injection: 1:1 FCA/FIA; 2 nd injection: 1:4 FCA/FIA; 3 rd injection: FIA. These adjuvant compositions were then mixed 1:1 with the antigen in PBS. Five control monkeys received the glutathione-S-transferase (GST) antigen produced in E. coli using the same protocol. The challenge infection was carried out injecting 2×10 6 red blood cells infected with an adapted Plasmodium vivax strain (Belem) 2.5 weeks after the final injection. The protection was evaluated by determining parasitemia daily in all animals by examining smears stained with Glemsa.
The curves in FIG. 5 show the variation in the measured parsitemia as the number of parasited red blood cells per microliter of blood (up the ordinate, logarithmic scale) as a function of the time passed after infection (in days). Curve A corresponds to the average values observed in the three vaccinated monkeys; curve B corresponds to the average values in the five control monkeys.
An examination of the Figure shows that the effect of the vaccination was to greatly reduce the parasitmisa.
Recombinant Plasmodium cynomolgi (p42 and p19) MSP1 Vaccination Test in the Toque Macaque Macaca sinica
Fifteen captured monkeys were used as follows: (1) 3 animals injected with 100 μg of soluble PcMSP1 p42 ; (2) 3 animals injected with 35 μg (1 st injection) or 50 μg (2 nd and 3 rd injections) of soluble PcMSP1 p42 ; (3) 3 animals injected with a mixture of PcMSP1 p42 and p19 ; (4) 3 animals injected with adjuvant plus PBS; (5) 3 animals not injected. Complete and Incomplete Freund adjuvant was used in the protocol described above. Injections were intramuscular at 4 week intervals. The challenge infection was made by injecting 2×10 5 red blood cells infected with Plasmodium cynomolgi 4 weeks after the last injection. Protection was evaluated by determining parasitemia daily in all animals by examining the parasitemia with Glemsa. Parsitemia were classified as negative only after counting 400 smear fields. The parasitemia were expressed as a percentage of parasitised red blood cells.
FIGS. 6A-6G show the results obtained. Each of them shows parasitemia (expressed as the percentage of parasitised red blood cells along the ordinate on a logarithmic scale) observed in the challenge animals as a function of the time after infection (in days along the abscissa).
The results relate to:
In FIG. 6A ; non vaccinated control animals; FIG. 6B relates to animals which received a saline solution also containing Freund adjuvant; In FIG. 6C is a superposition of FIGS. 6A and 6B , with the aim of highlighting the relative results resulting from administration of Freund adjuvant to the animals (the variations are clearly not significant); FIG. 6D provides the results obtained at the end of vaccination with p42; FIG. 6E concerns animals vaccinated with p19 alone; finally, FIG. 6F concerns animals vaccinated with a mixture of p19 and p42.

The p42 certainly induced a certain level of protection. However, as shown in FIGS. 6E and 6F , the protection conferred by the recombinant p19 of the invention was considerably better.
The hypothesis can be formulated that the improved protection resulting from secondary cleavage of p42 which is accompanied by revealing free cysteine which, as a result, forms intermolecular bridges giving rise to p19 multimers which are highly characteristic of this form in recombinant proteins of the three species tested.
The numbers used to produce graphs (6A-6F) are given in FIG. 6 G.
P. cynomolgi Torque Macaque Vaccination Test; Second Challenge Infection of Monkeys Vaccinated with p19 Alone and Controls ( FIG. 8 )
Six months later, with no other vaccination, the 3 macaques which received the p19 MSP-1 alone with FCA/FIA ( FIG. 6E ) and the 3 macaques which received a saline solution containing Freund adjuvant ( FIG. 6B ) and 2 new unaffected unvaccinated monkeys underwent a new challenge infection by injecting 1×10 6 red blood cells infected with Plasmodium cynomolgi. Protection was evaluated by determining parasitemia daily in all animals by examining Giemsa smears. The parasitemia were classified negative only after counting 400 smear fields. The parasitemia were expressed as the percentage of parastised red blood cells (the figures used to produce graphs 8 A-C are given in FIG. 8 D). The six immunised animals which underwent challenge infection six months earlier had no detectable parasitemia except for 1 animal in each group which exhibited a parasitemia of 0.008% for 1 day (FIGS. 8 A and 8 B). The two unaffected controls exhibited a conventional parasitemia with a maximum of 0.8% and for 21 days (FIG. 8 C). Thus the 3 animals vaccinated with the MSP-1 p19 were also protected for six months later than the 3 controls which exhibited a complete conventional infection after the first challenge infection, despite the absence of or a very slight parasmitemia after the first challenge infection. These results suggest that the protection period for p19 is at least six months.
Vaccination Test with p19 in Association with Alum in the P. cynomolgi Toque Macaque System ( FIG. 9 )
The previous positive protection results were obtained using complete (FCA) or incomplete (FIA) Freund adjuvant. However, the only adjuvant which is currently allowed in man is alum. For this reason, we carried out a vaccination test with P. cynomolgi MSP-1 p19 in the toque macaque in the presence of alum as the adjuvant. Six captured macaques were used as follows: (1) 3 animals injected with 4 doses of 50 mg of recombinant P. cynomolgi MSP-1 p19 with 20 mg of alum; (2) 3 animals injected 4 times with physiological water and 10 mg of alum. The injections were intramuscular at 4 week intervals. The challenge infection was made by injecting 2×10 5 red blood cells infected with P. cynomolgi 4 weeks after the last injection. Protection was evaluated by daily determination of parasitemia in all animals by examining Giemsa smears. The parasitemia were classified negative only after counting 400 smear fields. Parasitemia were expressed as the percentage of parasitised red blood cells. The results of this experiment were as follows. 2 of the 3 macaques immunised with recombinant p19 with alum had about 30 times less total parasitemia during the infection period ( FIGS. 9A and 9B ) then the 3 control macaques immunised with physiological water and alum ( FIG. 9D ) after the challenge infection. The third macaque immunised with p19 ( FIG. 9C ) was not very different from the controls. For the vaccination test using Plasmodium cynomolgi p19 in the toque macaque, macaca sinica, described in FIG. 9 , the data used to produce the graphs ( 9 A- 9 D) are given in (FIG. 9 E). While the results are a little less spectacular than the preceding results ( FIGS. 6 , 8 ), this is the first time that significant protection has been observed for recombinant MSP-1 with alum.
FIG. 10 : Vaccination Test with a Recombinant Plasmodium falciparum p19 in the Squirrel Monkey
Twenty Saimiri sciureus guyanensis (squirrel monkeys) of about 3 years old raised in captivity were used as follows: (1) 4 animals injected with 50 mg of soluble Pf MSP-1 p19 in the presence of Freund adjuvant as follows: 1 st injection: 1:1 FCA/FIA; 2 nd injection: 1:4 FCA/FIA; 3 rd injection: FIA. These adjuvant compositions were then mixed with 1:1 antigen in PBS; (2) 2 control animals received Freund adjuvant as described for (1) with only PBS; (3) 4 animals injected with 50 mg of soluble Pf MSP-1 p19 in the presence of 10 mg of alum (Alu-Gel-S, Serva); (4) 2 control animals received 10 mg of alum with only PBS; (5) 4 animals injected with about 50-100 MG of GPI anchored Pf MSP-1 p19 reconstitute into liposomes as follows: 300 mmoles of cholesterol and 300 mmoles of phosphatidyl choline were vacuum dried and resuspended in 330 mM of N-octylglucoside in PBS with 1.4 mg of Pf MSP-1 p19, GPI. This solution had been dialysed against PBS with adsorbent Bio-Beads SM-2 (Bio-Rad) and the liposomes formed were concentrated by centrifuging and resuspended in PBS The 1 st injection was made with fresh liposomes kept at 4° C. and the 2 nd and 3 rd injections were made with liposomes which had been frozen for preservation; (6) 2 animals injected with control liposomes made in the same way, in the absence of the p19, GPI antigen as described for (5); (7) 2 animals injected with physiological water. Three intramuscular injections were made at 4 week intervals. The challenge infection was made by injecting 1×10 6 red blood cells infected with Plasmodium falciparum. Protection was evaluated by determining parasitemia daily in all animals by examining the Giemsa smears. Parsitemia were expressed as the percentage of parasitised red blood cells. The results of this vaccination test are shown in FIGS. 10 , A-G.
The groups immunised with p19 in Freund adjuvant or liposome demonstrated similar parasitemia to the control groups after a challenge infection (one animal (number 29) vaccinated with p19 in Freund adjuvant died several days after challenge infection for reasons independent of vaccination (cardiac arrest”. Irregularities in administration of the antigen in these 2 groups (poor Freund emulsion, congealed liposomes) did not allow the significance of these results to be completely evaluated. In the alum group, 2 animals showed total parasitemia for the duration of the infection about 4 times less than the controls, 1 animal about 3 times less and 1 animal was similar to the controls. This experiment was a little difficult to interpret due to the variability in the controls, probably due to the strain of parasite used for the challenge infection which would not have been quite adapted to the non splenectomised Saimiri model developed only recently in Cayenne. However, the real effect with alum, although imperfect, is encouraging in that our antigens seem to be the only recombinant P. falciparum MSP-1 versions which currently have shown a certain effectiveness in combination with alum.
Vaccination Test with a Recombinant Plasmodium falciparum p19 in the Squirrel Monkey (Same Test as for FIG. 10 )
Monkeys bred in captivity were injected intramuscularly with 1 ml of inoculum twice at week 4 intervals as follows: (1) 4 animals injected with 50 μg of soluble PfMSP1p19 in the presence of Freund adjuvant as follows: 1 st injection: 1:1 FCA/FIA; 2 nd injection: 1:4 FCA/FIA, and mixed then 1:1 with the antigen in PBS; (2) 4 animals injected with 50 μg of soluble PfMSPp19 in the presence of 10 mg of alum; (3) 4 animals injected with about 50 μg of GPI anchored PfMSP1p19 reconstituted into liposomes composed of 1:1 molar cholesterol and phosphatidyl choline. The animals were bled 17 days after the second injection.
Red cells from a squirrel monkey with 30% parasitemia due to P. falciparum (with the mature forms in the majority) were washed with PBS and the residue was diluted 8 times in the presence of 2% SDS and 2% dithiothreitol and heated to 95° before being charged onto a polyacrylamide gel of 7.5% (separation gel) and 4% (stacking gel). After transfer to nitrocellulose, immunoblot analysis was carried out with antisera as follows: (1) pool of antisera of 4 monkeys vaccinated with soluble PfMSP1p19 in Freund adjuvant, twentieth dilution; (2) pool of antisera of 4 monkeys vaccinated with soluble PfMSP1p19 in alum adjuvant, twentieth dilution; (3) pool of antisera of 4 monkeys vaccinated with anchored PfMSP1p19 in liposomes, twentieth dilution; (4) monoclonal antibody, which reacts with a linear epitope of PfMSP1p19, 50 mg/ml; (5) SHI90 antisera pool originating from about twenty monkeys repeatedly infected with P. falciparum and which had become unaffectable by any subsequent infection with P. falciparum, five hundredth dilution; (6) antiserum pool of unaffected monkeys (never exposed to P. falciparum ), twentieth dilution.
The results show that the 3 antiserum pools of monkeys vaccinated with PfMSP1p19 reacted strongly and specifically with very high molecular weight complexes (diffuse in the stacking gel) and present in parasite extracts containing more mature forms. These results support the hypothesis that a specific aggregate of PfMSP1p19 is present in vivo comprising epitopes which are reproduced in recombinant PfMSP1p19 molecules synthesised in the baculovirus system, in particular olgiomeric forms thereof.
FIG. 7 also illustrated these results. It shows immunoblots produced on gel. The first three gel tracks illustrate the in vivo response of monkeys to injections of p19 [(1) with Freund adjuvant, (2) with alum, (3) in the form of a liposome] and in particular the existence of high molecular weight complexes supporting the hypothesis of in vivo aggregation of p19 in the form of an oligomer, specific to the maturation stage (when p42 is cut into p19 and p33).
This vaccination test also comprises a third injection identical to the previous injections. The injection with Freund adjuvant contained only FIA.
There were two animal controls for each group, namely: 2 control animals injected with PBS and Freund adjuvant; 2 control animals injected with PBS and alum; 2 control animals injected with liposomes without protein; and two control animals injected with PBS without adjuvant Protection was evaluated as described above.
FIG. 7 B: The data for this Figure were derived from the squirrel monkey P. falciparum I vaccination test ( FIG. 10 below). The numbers correspond to the individual monkeys noted in FIG. 10 . The techniques and methods for this Figure were the same as for FIG. 7 except that the individual antiserum for each monkey was tested after three injections the day of the proof injection and the SHI antiserum was diluted by 1:250. The results show that the antiserum for 4 monkeys vaccinated with p19 and alum reacted strongly and specifically with very high molecular weight complexes while the monkeys of other groups vaccinated with p19 and Freund adjuvant or liposomes showed only a little reactivity with these complexes. Since the monkeys vaccinated with p19 and alum were also the best protected, this reactivity with the high molecular weight complexes appeared to indicate a protective effect, despite one monkey in the group not being protected with respect to the controls and that another was only partially protected.
The invention also, of course, concerns other applications, for example those described below with respect to certain of the examples, although these are not limiting in character.
Therapy
The recombinant molecule PfMSP1p19 can be used to produce specific antibodies which can possibly be used by passive transfer for therapeutics for severe malaria due to P. falciparum when there is a risk of death.
Diagnostics
The recombinant molecules PvMSP1p42, PvMSP1p19 and PfMSPp19 derived from baculovirus can and have been used to produce specific murine monoclonal antibodies. These antibodies, in combination with polyclonal anti-MSP1p19 antisera originating from another species such as the rabbit or goat can form the basis of a semi-quantitative diagnostic test for malaria which can distinguish between malaria due to P. falciparum, which can be fatal, and malaria due to P. vivax, which is generally not fatal. The principle of this test is to trap and quantify any MSP-1 molecule containing the p19 portion in the blood.
In this context, the advantages of the MSP1p19 molecule are as follows:
(i) It is both extremely well conserved in the same species and sufficiently divergent between different species to enable specific species reactants to be produced. No cross reaction has been observed between antibodies derived from PfMSP1p19 and PvMSP1p19; (ii) the function of MSP1p19, while not known with precision, seems to be sufficiently important that this molecule does not vary significantly or is deleted without lethal effect for the parasite; (iii) it is a major antigen found in all merozolites and thus it must in principle be detectable even at low parasitemia and proportionally to the parasitemia; (iv) since the recombinant MSP1p19 molecules derived from baculovirus appear to reproduce more of the native structure of MSP1p19, the antibodies produced against these proteins will be well adapted to diagnostic use.

The microorganisms identified below have been deposited under Rule 6.1 of the Treaty of Budapest of 1 st Feb. 1996, in the Collection Nationale de Cultures de Microorganisms (C.N.C.M.) of Institut Pasteur at 28, rv du Dr. Roux 75724, Paris Cedex 15 under the following registration numbers:











Identification reference
Registration numbers





PvMSP1p19A
I-1659


PvMSP1p19S
I-1660


PfMSP1p19A
I-1661


PfMSP1p19S
I-1662


PcMSP1p19S
I-1663





















The invention also concerns the use of these antibodies, preferably fixed to a solid support (for example for affinity chromatography) for the purification of type p19 peptides initially contained in a mixture.
Purification means brining this mixture into contact with an antibody, dissociating the antigen-antibody complex and recovering the purified p19 type peptide.
The invention also concerns vaccine compositions, also comprising mixtures of proteins or fragment, in particular mixtures of the type:
P. falciparum p19 and P. vivax p19; P. falciparum p19 and P. falciparum p42, the latter if necessary being deprived of its most hypervariable regions; P. vivax p19 and P. vivax p42, the latter if necessary being deprived of its most hypervariable regions; P. falciparium p19 and P. falciparum p42, the latter if necessary being deprived of its most hypervariable regions, and P. vivax p190 and P. vivax p42, the latter if necessary being deprived of its most hypervariable regions.

In the present case, the most hypervariable regions are defined as regions I or region II and all or part of region III, the portion of region III which is preferably deleted being that which is juxtaposed to region II (the conserved portion being located to the side of the C-terminal of p33, close to the p19). Regions II and III are illustrated in FIG. 4 .
The invention is not limited to the production of human vaccine. It is also applicable to the production of veterinary vaccine compositions using the corresponding proteins or antigens derived from parasites which are infectious for mammals and products under the same conditions. It is known that infections of the same type, babesiosis, also appear in cattle, dogs and horses. One of the antigens of the Babesia species has a high conformational homology (in particular in the two EFG-like and cysteine-rich domains) and functional homology with a protein portion of MSP-1 [(36), (37) and (38)].
Examples of veterinary vaccines using a soluble antigen against such parasites have been described (39).
It goes without saying that the p19s used in these mixtures can also be modified as described in the foregoing when considered in isolation.
REFERENCES


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The invention also concerns hybridomas secreting specific antibodies selectively recognising the p19 of a MSP-1 protein in the merozoite form of a Plasmodium type parasite which is infectious for man other than Plasmodium vivax and which does not recognise Plasmodium vivax.
In particular, these hybridomas secrete monoclonal antibodies which do not recognise Plasmodim vivax and which specifically recognise Plasmodium falciparum p19.
The invention also concerns a hybridoma characterized in that it produces a specific antibody which specifically recognises the p19 of P. vivax and the p19 of P. cynomolgi. A F10-3 hybridoma has been constructed from the X63 Ag8 653 myeloma producing IgG 2b/k recognising the p42 glycoprotein of Plasmodium vivax.